LOCUS       DASSGX010000001         3038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_544551, whole genome shotgun sequence.
ACCESSION   DASSGX010000001 DASSGX010000000
VERSION     DASSGX010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544551"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..479)
                     /gene="rpsG"
                     /locus_tag="VFI12_00005"
     CDS             complement(9..479)
                     /gene="rpsG"
                     /locus_tag="VFI12_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HET7054806.1"
                     /translation="MPRRAKIVRRPLVPDARYNSEMVTRFTNKIMQRGKKGLAERILY
                     GALDIIAQRSGRNPLEVFEQAVHNATPVIEVKPRRVGGATYQVPVQIDQQRRVSLAIR
                     WLLTAAKARSGKSMQEKLAGELLDAANGTGATIKRREDTHRMAEANRAFAHYRY"
     gene            complement(646..1077)
                     /gene="rpsL"
                     /locus_tag="VFI12_00010"
     CDS             complement(646..1077)
                     /gene="rpsL"
                     /locus_tag="VFI12_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003225781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HET7054807.1"
                     /translation="MPTINQLVRKGRKSVKKKTKAPALGYTYASVGRYAGRQRAGSSA
                     PQKRGVCTQVKTMTPKKPNSALRKIARVRLSNQMEVTAYIPGEGHNLQEHSVVLVRGG
                     RVKDLPGVRYHIVRGTLDARGVENRKQARSKYGTKAPKKAK"
     gene            1321..2166
                     /locus_tag="VFI12_00015"
     CDS             1321..2166
                     /locus_tag="VFI12_00015"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7054808.1"
                     /translation="MPDLLQDWFRVWEDRPGTWVIEEPLHSEEVKSYLVLGRDRAALI
                     DTGMGVGDIRRLVEQITSLPISVIQSHAHNDHIGGAWQFDDVGIHPSEADDLAAGQSA
                     ERLTRWFAESEMSGPLPPGFDPVGYSIPGKSPNRTYDDGDIIDLGGRTLEVIHAPGHS
                     RGGLVLLDRANRDLYSTDVVYLRSLYLLNPDSSVSDYVATLERLAALVPNLDRLYPAH
                     GPSPISPDLIPAMLDGMREIALGREPDQIEHEPAIEGGNGSHINRSALHIHEFGPYRV
                     LVAIA"
     gene            complement(2171..2701)
                     /locus_tag="VFI12_00020"
     CDS             complement(2171..2701)
                     /locus_tag="VFI12_00020"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7054809.1"
                     /translation="MKAVEAEGVAGRTLLDIGGGVGAISHELLRSGLAHATSIDAAGA
                     YVAAARSEAERQGHADRLMLQQGDFVLLADDIPAVDIVTLDRVICCYPDMPALVGSSV
                     AHARQVYGLVYPRDTWWTRLGVRMVNLYFRARRHTFRTFVHSPESVDALVTAHGFRIR
                     FRQRTSVWQVVVYARP"
BASE COUNT          542 a          974 c          927 g          595 t
ORIGIN      
        1 atgtccgcct agtagcggta atgcgcgaag gcgcggttgg cctcggccat gcggtgcgta
       61 tcctcgcgcc gcttgatcgt ggcgcccgtg ccgttggcgg catcaagcaa ttctccggcc
      121 agcttctcct gcatcgactt accggagcgc gccttggcgg cggtgagcag ccagcggatc
      181 gcgagcgaaa cgcgacgctg ctggtcgatc tgaaccggaa cctggtaggt cgcgccaccc
      241 acgcggcggg gcttgacctc gatgaccggc gtcgcgttgt ggacggcctg ctcgaagacc
      301 tcgagcggat tgcggccgct gcgctgcgca atgatgtcga gcgctccgta gaggatccgc
      361 tcggccaggc ccttcttacc ccgctgcatg atcttgttgg tgaaccgggt caccatctcg
      421 ctgttgtacc gggcatcggg gaccagcggc cgtcgaacaa tctttgcgcg tcgtggcatg
      481 gcgtcgttcg cctccaaagc gacccggact tcacgttccg ggaatgatca aaacttccaa
      541 aagttcgtga cacacgaaaa agcggtcaac gctcaccgtt tgagacgtca agccgctttg
      601 tcgtgcattc tcagttccgc aagtgcggag aggtgtcgag ggggcctact tggccttctt
      661 cggcgccttg gtaccgtact tcgaccgagc ctgtttccga ttctcaacgc cacgggcatc
      721 cagggtgcca cggacgatgt ggtaacgaac gccgggcaag tccttgactc gcccaccgcg
      781 taccagcacc accgagtgct cctgaaggtt gtgcccctcg cctgggatat acgccgtcac
      841 ttccatttga ttggacaagc gaacccgggc aattttccgc agcgcggaat tcggcttttt
      901 gggcgtcatc gtcttgacct gggtgcagac accgcgcttt tgcggagcgc tgctcccggc
      961 ccgttgccga ccggcatacc gacccacgga tgcataggtg tatccgaggg ctggcgcctt
     1021 cgtcttcttc ttgacgctct ttcggccttt acggaccaac tgattaatcg ttggcacgac
     1081 aaaactcctt aaaggacaga tggcttgtgt tcccgtgctc tgcgtgccgc actcgctcgc
     1141 agctgcggat ccatttgccc tgctcgtaga cccacaaaac ccgcgtcctt gccagtcagt
     1201 gggacacgca gcaatgtccg cccgtcgggc ggacacaagg ggtaataata cgcgctgcga
     1261 gattggtcgt caaggctgga tggatatgtc gggccgtctg gccgtaagga gcgagcgcgc
     1321 atgccggatc tgctgcaaga ctggtttcgg gtttgggaag accggcccgg aacatgggtg
     1381 atcgaggagc cgctccacag cgaagaagtc aagtcgtacc tcgtcctggg acgtgatcgg
     1441 gctgctctga tcgacaccgg gatgggcgtc ggcgacattc gccgactggt cgagcaaatc
     1501 acctcactgc cgatatccgt catccagagc catgcccaca atgaccatat cggcggcgcc
     1561 tggcaattcg acgacgtcgg catccacccg tcggaagcgg acgatcttgc ggccggtcag
     1621 tctgcggagc gcctcacgcg ctggttcgcg gagtccgaga tgagcggtcc gctcccgccg
     1681 ggcttcgatc ccgttggcta ttcaattccc ggcaaatcgc cgaaccggac ctatgacgat
     1741 ggagacatca tcgatctcgg gggccgaacg ctcgaagtaa tccatgcgcc gggtcactcc
     1801 cgtggtgggc tggtgctcct ggaccgagcc aaccgggacc tgtactccac ggacgtcgtc
     1861 tacctccggt cgctttatct gctcaacccg gattcgtcgg tttccgacta tgtcgccacc
     1921 ctggagcggc tggcggcact cgttcccaac ctcgatcggc tgtatcccgc gcatggtccg
     1981 agcccgatct cgccggatct gattccggct atgctcgatg gcatgcggga aatcgccctg
     2041 gggcgcgagc cggatcagat cgagcacgaa ccggccattg agggcggcaa cgggagccac
     2101 ataaatcggt ctgccttgca tattcacgag ttcggcccat accgggtgct cgtcgcgatt
     2161 gcgtgacgat tcagggtcga gcgtagacca cgacctgcca gacgctcgta cgttgccgaa
     2221 aacggattcg gaacccgtgt gcagtcacga gggcgtcgac cgactccggc gagtggacga
     2281 acgtccggaa ggtgtggcgg cgagcccgga aatacaggtt gaccattcgg actcccaatc
     2341 gggtccacca ggtgtcgcgc gggtagacca acccgtaaac ctgcctcgca tgcgccacgg
     2401 aagagccgac gagcgccggc atgtcgggat aacagcagat cacccggtcg agagtaacga
     2461 tgtcgaccgc cggaatgtcg tcggcgagca ggacgaagtc gccctgctga agcatcagac
     2521 gatccgcgtg accctgccgc tcggcttcgg accgcgcagc cgcgacatat gcgccggcgg
     2581 catcgatcga cgtcgcgtgt gcgaggcccg accgaaggag ctcgtgcgag atcgctccca
     2641 ccccaccgcc gatatcgagt agcgtccggc ccgctacgcc ttctgcttcc accgccttca
     2701 acagtgcgcg cgtagtcctc gacggtccgc gtcggcggta ttggcgaagg tttatggccg
     2761 cgattttctt gccgaactcg gcatccaggg actgcgcccg gcaacagtcc atcttgatcc
     2821 tcccactctg gcaattggcc cgctatggcc ccgcatgatt tcgaaccgag attgcttcca
     2881 aagaatacga aacgggacgg gcgcactgcc cgtcccgttt cgattttgca cgctgttcgg
     2941 cgctggacta gttgatgtcc tgcgcctcca gatcctcgac tgtcgcgacc gagtacccgt
     3001 ccgcgccatc gccgctcacg tcatcggtcg ggagcgcg
//
LOCUS       DASSGX010000002         3242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_979986, whole genome shotgun sequence.
ACCESSION   DASSGX010000002 DASSGX010000000
VERSION     DASSGX010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_979986"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..1110)
                     /locus_tag="VFI12_00025"
     CDS             complement(61..1110)
                     /locus_tag="VFI12_00025"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HET7054810.1"
                     /translation="MAQVQEQARVSADAGDVASAGSTQKTYIDAIHDTLQQEMRADER
                     LVILGEDVGRRGGVFRVTAGLLDEFGSERVIDTPLAESAIIGTAIGMSLNGLRPIAEI
                     QFLDFIHSAIDQIMSEAARIRYRSNNDFSCPIVIRVPYGGGVHGALYHSQSIEAIFVH
                     TPGLKVVAPATPADAAGLLRAAIHDEDPVLYLEHKKMYRAIKGDVPGSDYEVPIGSAR
                     IVQDGTDATVITYGMMVHEATKAAAIVSEETGASIEIVDLRSLWPLDEAVILESAKKT
                     GKVLVVTEANEPCSVASEVAAMVGEDAFAWLDAPVMRLAAPDVPAMPFSPPLEHAFLI
                     DADKIGDALRRLISY"
     gene            complement(1189..2307)
                     /locus_tag="VFI12_00030"
     CDS             complement(1189..2307)
                     /locus_tag="VFI12_00030"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HET7054811.1"
                     /translation="MTTDATKSPAKAVQGAFDPPFTHGYLPPQSGDFQAATLSAHVQP
                     SSKGDAGLSPDLLRDMFRTLSLARALDERMWQLNRSGKAAFIVSGQGHEGAQIGAGFA
                     LERGKDFIVPYYRDLALCLSFGLTPTDVMMAALAKAPDPASGGRQMPAHYGSARLRII
                     TSSSPVSTQIPHAVGIAWAAKVRGTDEVALTCFGEGGSSKGDFHESCNFAGIHKLPVI
                     FFCENNQYAISVPVSEEVAIEHVADRAAGYGFPGVVVDGTDVLAVYQAVKGAADRARN
                     GEGATLIEAKVYRFQPHTSDDDDRTYRDPEEVKAWKGHDPLALFERRLRECGVLDDDL
                     VKHIRTEVMRDVNEATDAADAAPYPDPSTFDRHVYAER"
     gene            2573..>3242
                     /locus_tag="VFI12_00035"
     CDS             2573..>3242
                     /locus_tag="VFI12_00035"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7054812.1"
                     /translation="MSMPLTFELSEEQRALRDTIREFAEAEIAPHAAEWDQSHTFPIE
                     TVRKLGELGAMGVAFPEEYGGLGMGAVAQAVVVEELARVDSSIAVTVGADVSIAGEPI
                     LRFGTEVQKQRWLVPLARGETLGAFASTEPDSGSDVRSAKTTARRENGCWVINGTKAF
                     TTNAGTEMSAFVIATAQTAFDEGRKLSTFVIPQGTPGYEPKKPYHKMGWHGSDTRELV
                     FTDVR"
BASE COUNT          603 a         1057 c         1025 g          557 t
ORIGIN      
        1 ctctcgccaa gctgcggcat ggtaatggtg gtcagcgcct tggtcggcat cgcaccctcc
       61 ctagtagctg atcaaccggc gcagcgcgtc gccgatcttg tccgcatcga tcaggaaggc
      121 gtgctccagg ggaggactga agggcatcgc cggcacatcg ggcgcggcca ggcgcatcac
      181 aggagcgtcg agccaggcaa aggcatcctc gccgaccatc gccgcaactt cgctggcgac
      241 agagcacggc tcgttcgcct cggtgacgac cagcaccttg ccggtcttct tcgccgactc
      301 gaggattact gcctcatcga gcggccaaag ggagcgaaga tcgacgatct cgatgctcgc
      361 gccggtctcc tcagatacga tcgcggccgc ctttgtcgcc tcgtggacca tcatcccgta
      421 ggtgatgacc gtcgcatcgg tcccgtcctg gacgatccgc gccgaaccga tcggcacctc
      481 gtaatcgctg ccggggacgt cgcccttgat cgcccggtac atctttttgt gctcgaggta
      541 gaggaccgga tcctcatcat gaattgcggc ccgaagcagg ccggcggcat cggccggtgt
      601 ggccggcgcg acgaccttga gcccgggcgt gtggacgaaa atggcttcga tgctttgcga
      661 gtggtaaagc gcgccgtgca ctcccccgcc atagggcacg cggattacga tcggacaaga
      721 gaagtcgtta tttgagcggt accggattct ggctgcctcg ctcatgatct ggtcgatagc
      781 ggagtggatg aaatcgagga actggatttc ggcaatcggg cggaggccat tcaatgacat
      841 tccgatcgca gtgccgatga tcgccgattc cgccagtgga gtgtcgatca cccgctccga
      901 gccgaactca tcgagcaggc cggccgttac ccggaagacg ccgccccgcc ggcccacatc
      961 ctcgccgagg ataaccagcc gttcgtccgc tcgcatctcc tgctgaagcg tgtcgtggat
     1021 ggcgtcgatg tacgtcttct gcgttgagcc ggctgacgcg acgtcgcccg catcggccga
     1081 caccctagcc tgttcctgta cctgggccat ttcacatcct cacgatgacg ggcaaggcga
     1141 cgaatcgccg ctgcgttcga aagcgacgcg aagtgatcga atccatggct accgctccgc
     1201 gtagacgtgc cgatcgaacg tcgatggatc ggggtacggc gccgcgtcgg ccgcgtcggt
     1261 cgcctcattg acgtcgcgca tcacctcggt tcggatgtgc ttgaccaggt catcgtcgag
     1321 cacgccgcac tcccggaggc gacgctcgaa gagtgcgagc ggatcgtgcc ccttccacgc
     1381 cttgacctct tccgggtcgc ggtaggtgcg gtcgtcatcg tccgaagtgt gcggctggaa
     1441 ccggtagacc ttggcttcga tcagcgtggc cccctcgccg ttgcgagcgc ggtctgccgc
     1501 tcccttgacc gcctggtaga cggccaggac gtcggtgcca tcgacgacga cgcccgggaa
     1561 gccgtatccc gcagcgcggt cggcgacgtg ctcgatcgcc acttcctccg aaaccggcac
     1621 cgagatggcg tactggttgt tctcgcagaa gaagatgacc ggcaacttgt ggatcccggc
     1681 gaagttgcac gactcgtgga aatcgccctt ggacgagccg ccctcgccga aacaggtcag
     1741 cgcgacctca tcggtcccgc gcaccttcgc cgcccaggca atgccgaccg cgtggggaat
     1801 ctgcgtcgag acggggctcg agctggtgat gatccggagc cgagccgagc catagtgggc
     1861 tggcatctgt ctgccgccgg aagccggatc cggcgccttg gccaacgccg ccatcatcac
     1921 gtcggtcggt gtcaagccga acgacagaca cagggccagg tcgcggtaat acgggacgat
     1981 gaaatccttg ccgcgctcaa gagcgaaccc ggcgccgatc tgcgcgccct catgcccctg
     2041 cccggagacg atgaacgccg ccttgccgga ccggttgagt tgccacatcc gctcatcaag
     2101 cgcccgggcg agcgacagcg tcctgaacat gtcgcgcagg agatcaggcg acaaaccggc
     2161 atcacccttc gacgacggtt gaacgtgcgc agagagagta gccgcctgga aatcgccgct
     2221 ttgaggggga agataaccgt gggtgaaggg gggatcaaat gcgccctgga cagctttggc
     2281 cggggatttg gttgcatcgg tcgtcattga ccgcacctcg cagacttgag agtcggccac
     2341 cttgcttgcc aaaccggccg aatacatgac gtctcgtctt cgatcatagc gcaattacac
     2401 aagaccgccg gttcgccagg aacctgtcgg ctggattcac cgcgagacgt ccgtacctgt
     2461 cgatttcggg taacggaatt tgcggaacgt aacaaagcag gcgatatact cggaaccaga
     2521 ggtccgcaac ggcgcggccc gatggtccac gattcgagac cgggtgacga ccatgtccat
     2581 gcccctcacg ttcgagctca gcgaagagca acgcgccctc cgcgacacga tccgcgaatt
     2641 cgccgaagcg gaaatcgcgc ctcacgcggc cgagtgggac caatcccaca ccttcccgat
     2701 cgagacggtc cggaagctcg gtgagcttgg cgcaatgggc gtcgccttcc cggaggaata
     2761 cggcgggctc gggatgggcg cggtggccca agcggtcgtg gtcgaggagc tggcccgggt
     2821 cgactcgtcg atcgcggtga ccgtcggcgc cgatgtctcg atcgccggcg agccgattct
     2881 ccggttcggt accgaagtgc aaaagcagcg ctggcttgtc ccgcttgccc gaggcgagac
     2941 cctcggcgca ttcgccagca ccgaaccgga ttccggatcg gacgtccgca gcgcgaagac
     3001 caccgcccgc cgcgaaaacg gctgttgggt catcaacggg acgaaggcgt tcacgaccaa
     3061 cgccggaacc gaaatgagcg ccttcgtgat cgcgacggcg cagaccgcct tcgacgaagg
     3121 tcgcaagctc agcacgttcg tcatccccca aggcacaccc ggctacgagc ccaagaagcc
     3181 atatcacaag atgggctggc acggctccga cacgcgggag ctcgtcttca ccgacgttcg
     3241 cg
//
LOCUS       DASSGX010000003         2021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1306499, whole genome shotgun sequence.
ACCESSION   DASSGX010000003 DASSGX010000000
VERSION     DASSGX010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306499"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VFI12_00040"
     CDS             complement(<1..188)
                     /locus_tag="VFI12_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HET7054813.1"
                     /translation="MADRLPPQNLEAEQSVLGSLLIDRDAIIQVAPILKHDDFYQPSH
                     GTIYQAITELYARQEPTDV"
     gene            complement(269..754)
                     /gene="rplI"
                     /locus_tag="VFI12_00045"
     CDS             complement(269..754)
                     /gene="rplI"
                     /locus_tag="VFI12_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HET7054814.1"
                     /translation="MKIILRKDVEKLGEAGSLATVKDGYARNYLIPQGMAIAATPGEL
                     KLWEHNQAVKTRKVERQERELQSFADKISGQTLTFEARAGEGGRLFGSVTAADIAEKL
                     SSAVGEEIDRRKVVLSEPIRSTGEHTVTVNVIGKLRPQVKVIVNGVVDEADEDTAEAT
                     A"
     gene            complement(878..1702)
                     /gene="rlmB"
                     /locus_tag="VFI12_00050"
     CDS             complement(878..1702)
                     /gene="rlmB"
                     /locus_tag="VFI12_00050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HET7054815.1"
                     /translation="MRTDRKAPPGEKRAKPRRPSRDEGKDRIYGRNAVVATLDGKRHV
                     HGILLAEGVGQDARIGTIREIAERRRIPIQTVPVQGIENIVGKVNHQGVLATTDRYGY
                     ASLRSVMDRPGTILVLDHITDPQNLGTLLRAARAFDIAGVVIPSDRSASVTPAVVNAS
                     AGAVETLPVAQVVNIARAIGEIREHGRWVAGLDTGADSQSIYTTEIPLPVALVLGSEG
                     RGISRQVRAACDLVISIPISDQVESLNVATAGSITLFELARRMFGAHSGRVSSGSN"
     gene            complement(1659..>2021)
                     /locus_tag="VFI12_00055"
     CDS             complement(1659..>2021)
                     /locus_tag="VFI12_00055"
                     /inference="COORDINATES: protein motif:HMM:NF020752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR domain-containing protein"
                     /protein_id="HET7054816.1"
                     /translation="RADFEAAMDDDFDTPGAVAAIFNLARAINRSAGDVGSADTLVAA
                     RETLQGLLEVLGIDPDADADSAVMGAAPFVELLLQIRDQLRAGKHWVEADAIRNGLNE
                     LGIRIEDGPEGATWRKES"
BASE COUNT          332 a          645 c          648 g          396 t
ORIGIN      
        1 acgtccgtcg gctcctgtcg cgcatagagc tcggtgattg cctggtagat ggttccgtga
       61 gagggttggt agaagtcgtc gtgcttgaga attggcgcca cctggatgat ggcgtcgcga
      121 tcgatcagca agctgccgag cactgattgc tcggcttcga ggttctgtgg tggaagtcgg
      181 tcagccattg gcccctaccc tggaagcgtc ctgccaagac gggaagcagg atcccagcgt
      241 cacctggcgg ggtcaggctg tcaaaagtct atgcggtcgc ttccgcggtg tcctcgtcgg
      301 cctcgtcaac gacgccattg acgatgacct tgacctgcgg ccgcagcttt ccgatgacgt
      361 tgacggtgac ggtgtgctcg ccggtcgacc ggatgggctc ggacaggaca accttgcggc
      421 gatcgatctc ctcgccgacg gcagacgaga gtttctccgc gatatcggcg gcggtgacgg
      481 agccaaacag acggccaccc tcgccggcac gggcctcgaa ggtcagagtc tggccggaga
      541 tcttatcggc gaaagattgc agctcgcgct cctggcgctc gaccttgcgg gtcttgaccg
      601 cctggttgtg ctcccacagc ttgagctcgc cgggcgtcgc cgcgatagcc atgccctgcg
      661 ggatcaggta atttcgggcg tatccgtcct tgacagtggc caaggagcct gcctcgccga
      721 gtttctcaac gtccttgcgc agaatgattt tcatcgtgag cggaaccttc caatgcccgg
      781 gggctgggtt tggcgccgat tctgcgccag atttgagaca caacacgaga cggaccgcgg
      841 tccggccaac caattctagc ggcagaaccg gttgaaatca attcgagccg ctcgaaaccc
      901 gacccgaatg agcgccgaac atgcgccgcg cgagctcgaa cagggtgatc gacccggcag
      961 tggcgacgtt cagcgattcc acctggtcgc tgattgggat cgagatgacc aggtcgcacg
     1021 cggctcgcac ctgccggctg atgccgcgcc cttccgatcc aaggaccagg gccaccggca
     1081 gcgggatctc ggtcgtgtag atcgactggc tgtctgcgcc ggtgtcgagc ccagccaccc
     1141 agcggccgtg ctcgcggatc tcgccgatcg cacgggcgat gttcacgacc tgggcgaccg
     1201 gcagtgtttc caccgcgccg gccgatgcat tgacgaccgc cggtgtgacc gaggctgacc
     1261 ggtccgacgg gatgaccacg cctgcgatgt cgaacgcccg ggccgcgcgc agcaatgtcc
     1321 cgaggttctg cggatcggtg atgtggtcca gcacgaggat cgttcccggc cggtccatca
     1381 cgctgcgcag tgacgcatac ccgtagcggt cggtcgtggc gagcacgccc tggtgattga
     1441 ccttcccgac gatattctcg atcccctgga ccggaacggt ctggatcggg attcggcgtc
     1501 gttccgcaat ctcgcggatg gttccaatgc gcgcatcctg accgacgcct tcggcgagca
     1561 ggatgccgtg gacgtgtcgc ttgccgtcca gtgtggcgac gacggcattg cgaccataga
     1621 tgcggtcctt gccctcgtct cggctcggcc ggcggggttt agctctcttt tcgccaggtg
     1681 gcgccttccg gtccgtcctc aatgcgtatc cctaactcat tcagtccatt tcgaatcgcg
     1741 tcggcttcga cccaatgctt gcccgcccgg agttgatcgc gaatctgcag tagcagctcg
     1801 acgaatggcg ctgcgcccat gactgccgaa tcggcatcgg cgtccgggtc gattccgagc
     1861 acctcaagca gaccctgcag cgtttcccgg gcggctacca gtgtgtccgc ggatcccacg
     1921 tccccggccg agcgattaat ggcccgggcg aggttgaaga ttgcggcgac ggcccccggc
     1981 gtgtcaaagt cgtcgtccat tgccgcttcg aaatcggccc t
//
LOCUS       DASSGX010000004         3091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1197755, whole genome shotgun sequence.
ACCESSION   DASSGX010000004 DASSGX010000000
VERSION     DASSGX010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1197755"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..1844
                     /locus_tag="VFI12_00060"
     CDS             132..1844
                     /locus_tag="VFI12_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HET7054817.1"
                     /translation="MTEIDRREYDASFGPTTGDRIRLADTNLFIEVEEDRCVGGDEAI
                     FGGGKTIRESMGQSMATRAEGTPDLVITGAVVLDHWGVIKADVGIRDGRITALGKAGN
                     PETMDGVHPDLVIGPSTEVAAGHGMILTAGAVDTHVHYIDPDELVEALGAGITTVAGG
                     GTGPTEGSKATLATPGSWWLERMLESFDPWPLNVLFLARGNTVSHAALWEQLRGGAAG
                     FKVHEDWGSTPAAVDAALTVAEEAGVQVAIHSDTLNEAGFVEDLLAAANGRTFHAYHT
                     EGAGGGHAPDIIRIAGEPNVLPSSTNPTRPFTRNTVAEHLDMVMVAHQLNPNVPNDLA
                     FAESRVRPTTIAAEDVLQDMGAISVMSSDAQAMGRIGEVIIRTWQTAHQMKKLRGALP
                     GDGRADNHRARRYVAKYTICPAIVHGMEKEVGSVEPGKLADLVLWHPAFFGVRPATVF
                     KGGMVALAAVGDPNASVPTPQPVLPRLGFNAHSPAAAATSVSFVSQAALDDGIADRLT
                     RVQRPLVAIQNVRGRTKADLPENTALPRIEVDSDTYSVRIDGELIEHEPATELPMAQR
                     YFLF"
     gene            1847..2569
                     /locus_tag="VFI12_00065"
     CDS             1847..2569
                     /locus_tag="VFI12_00065"
                     /inference="COORDINATES: protein motif:HMM:NF013858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HET7054818.1"
                     /translation="MADPAITFDHRPSHSARSALLLLADGRLPAGGHAHSGGLEAAAA
                     LEGVRDPATLESFLRGRAATAGMVAGAFAAASCAAFEHRGVSNLVQLDTELNARIPSP
                     TLRAVSRRLGRNMLRTGRAIWPHPGLDDVAADLDRSPHHPVALGAVAAAAGLSPDEVA
                     AAAMHDAVAGPATAAVRLLGLDPLAVHAILGKLGAELDALAANAAAMCDRPAPQLPAF
                     SSPVLDLLSEAHATWEVRLFAS"
     gene            2559..>3091
                     /gene="ureG"
                     /locus_tag="VFI12_00070"
     CDS             2559..>3091
                     /gene="ureG"
                     /locus_tag="VFI12_00070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HET7054819.1"
                     /translation="MHPEPTHPNGNTPHVHPDRLSSLPRVSGKPLRIGIGGPIGSGKT
                     ALVAALCRALGSELNLAVVTNDIYTTEDADFLLRNGVLPAERVRAVETGCCPHTAIRD
                     DISANLDAIEDLEDHLPDLELILIESGGDNLTATFSRGLVHYQIFVLDVAGGDKVPRK
                     GGPGVSTADLLVINKTDL"
BASE COUNT          508 a         1099 c         1008 g          476 t
ORIGIN      
        1 cagacgcctc aacgtccttg ccggcggttc ggtgcgattt gagcccggcg cggtcgaaga
       61 ggtagagctg attccaatcg gcgggaagcg catcgttgcg ggcttgcgcg gggagtgcgg
      121 agggaagctc gatgaccgag attgatcgcc gcgaatacga tgcctccttc ggcccgacga
      181 cgggcgaccg catccggctg gcggacacca acctcttcat cgaggtcgag gaagaccgct
      241 gcgtcggtgg agacgaggcc atcttcggcg gcggcaagac gattcgcgag tccatggggc
      301 aatccatggc aacccgcgcc gagggcacgc cggacctggt catcactggc gccgtcgtac
      361 tcgaccactg gggtgtcatc aaggccgatg tcggaatccg cgacggccgg atcacggcgc
      421 tgggcaaggc gggcaatccc gaaaccatgg atggcgtcca tcctgatctg gtcattgggc
      481 cgtccaccga agttgcggcc ggccacggca tgatcctgac ggccggggca gttgatacgc
      541 acgtccacta cattgacccg gacgagctcg tcgaggcact gggagcgggc atcactaccg
      601 tcgccggtgg cggcaccggg ccaaccgagg ggtcaaaggc gaccctggcc acacctggtt
      661 cctggtggct cgagcgaatg ctggagtcgt tcgatccgtg gccgctcaat gtcctgttcc
      721 tggcccgggg caatacggtc tcgcacgccg cgctctggga gcagttgcgc ggcggagcgg
      781 ccggcttcaa ggtccacgag gactggggct cgacaccggc ggccgtcgac gccgccctga
      841 ccgttgccga agaggccggc gtgcaggtgg cgatccatag tgacacgctc aacgaggcgg
      901 gattcgtcga ggacctgctc gccgccgcca atggccgtac ctttcacgcg taccacaccg
      961 aaggcgccgg cggtggccat gccccggaca tcatccggat cgccggggaa cccaacgtgc
     1021 tgccctcgtc caccaacccg acccgcccct ttacccgcaa caccgtagcc gagcatctcg
     1081 acatggtcat ggtggcgcac cagctcaacc cgaacgtgcc gaacgacctt gcctttgccg
     1141 aaagccgggt gcgcccgacc accattgccg cggaggacgt cctccaggat atgggcgcaa
     1201 tttcggtcat gtcctccgac gcgcaggcca tgggccgcat cggcgaagtc atcatccgga
     1261 cctggcaaac cgcccatcag atgaagaaac tgcggggcgc gctgccgggc gatggccgcg
     1321 ccgacaacca ccgggcgcgg cgatacgtcg ccaagtacac gatctgtccg gcgatcgtcc
     1381 acgggatgga gaaggaggtt ggctcggtcg agccgggcaa actcgcggac ctcgtactct
     1441 ggcacccggc attcttcggc gtccgcccgg caacggtgtt caagggcggc atggttgccc
     1501 tcgccgccgt gggagacccc aatgcctccg tgccgactcc ccaacccgtc ctgccccggc
     1561 tgggcttcaa cgcgcacagt ccggctgccg cggcaaccag cgtctccttc gtgtctcagg
     1621 ccgcgctcga cgacgggatc gccgaccgac tgacccgggt gcagcgcccg ctggtcgcca
     1681 tccagaacgt gcggggacgg accaaggccg acctgccgga gaacactgcg ctgcccagga
     1741 ttgaggtcga ctccgacacc tacagcgtcc gaatcgacgg cgagctgatc gagcacgagc
     1801 cggcaaccga gctgccaatg gcgcagcgct acttcctttt ctgacgatgg cagacccagc
     1861 gattaccttc gaccatcggc ccagtcattc ggcacgctcg gccctcttgc tactcgccga
     1921 cgggcgactg ccggccggcg gccatgcgca ctcgggaggg ctcgaggccg cggccgccct
     1981 ggaaggggtg cgcgacccgg cgaccctgga gtcgttcttg cgcggacggg cggcgaccgc
     2041 cggcatggtg gccggcgcct tcgcggccgc cagttgcgct gcgttcgagc atcgcggtgt
     2101 cagcaatctc gtccaactgg acacggagct caacgcgcgg atcccatcgc cgacgcttcg
     2161 cgccgtttcc cgccgccttg gccggaacat gctccggacc ggacgcgcca tctggccgca
     2221 cccgggactg gacgatgtcg ccgccgacct ggaccggagc ccgcatcacc cggttgccct
     2281 cggcgcggtg gccgccgccg ccgggttgtc gccggacgag gtcgcggcag ccgccatgca
     2341 cgacgccgtc gccggaccgg ccaccgccgc ggttcgcctg cttggcctcg acccgcttgc
     2401 cgtccacgca atcctgggca agctcggtgc ggagctggac gcactggccg ccaacgccgc
     2461 cgcgatgtgc gatcgtccgg cgccgcagct gcccgcgttc agttcgccgg tgctcgatct
     2521 gctctccgaa gcgcacgcga catgggaggt acgtctcttt gcatcctgaa ccaacgcatc
     2581 ccaacggaaa caccccgcac gtccacccgg accggctgtc gtcccttccg cgtgtctctg
     2641 gcaagccgct gcgcatcggg atcggtgggc cgatcggctc cggcaaaacg gcgctggtcg
     2701 cggccctttg ccgagcgctc ggctccgagc tgaatctggc agtcgtcacc aacgacatct
     2761 acaccacgga agacgccgat ttcctgctcc gcaacggcgt ccttcctgcc gagcgggtgc
     2821 gtgccgtgga aacgggctgc tgcccgcaca ccgccatccg cgacgacatc tccgccaatc
     2881 tcgacgccat cgaggacctc gaagaccacc tgccggatct tgagctcatc ctcatcgaga
     2941 gcggcggcga caacctgacc gcgaccttca gccgggggct ggtgcactac cagatcttcg
     3001 tgctcgacgt cgccggtggc gacaaggtgc cgcggaaggg cggaccgggc gtgtccacgg
     3061 ccgacctgct ggttatcaac aagacggacc t
//
LOCUS       DASSGX010000005         3057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_327307, whole genome shotgun sequence.
ACCESSION   DASSGX010000005 DASSGX010000000
VERSION     DASSGX010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327307"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VFI12_00075"
     CDS             <1..279
                     /locus_tag="VFI12_00075"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET7054820.1"
                     /translation="CPQETRMNPVLWIVFGAIAGWIAGRLYGDDRPEGCVTNIVLGIG
                     GALLGGAIYTLITGRDFTAGFNLPSLIVAILGALLLILILRRLGRGRH"
     gene            454..1512
                     /locus_tag="VFI12_00080"
     CDS             454..1512
                     /locus_tag="VFI12_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HET7054821.1"
                     /translation="MIRPLSTLFGFFSRDLGIDLGTANTLVYVRGKGIVISEPSVVAI
                     DTKTKRALAVGVEGKKMVGKTPESIIAVRPLKDGVIADFDTVEMMLHYFIRKVHTQRM
                     MAPHPRVVVGIPSGVTEVEKRAAKDAALSAGAREAYTIEEPMAAAIGAGLPINEPVGS
                     MIVDIGGGTTEVAVISLGGIVINHSIRVAGDEIDDAIIQYARRDHNLVIGERTAENAK
                     IAAASAYPLEEEIRVVLRGRDLLTGLPKSVEISSVELREAISPPVNTIVELVKTAIEE
                     TPPELVADIMEQGITLAGGGALLHGLDRRLQNELKMPVIRAEDPLTCVARGTGRVAEA
                     LHLYERALAGGRELRRAS"
     gene            1573..2388
                     /gene="mreC"
                     /locus_tag="VFI12_00085"
     CDS             1573..2388
                     /gene="mreC"
                     /locus_tag="VFI12_00085"
                     /inference="COORDINATES: protein motif:HMM:NF016011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HET7054822.1"
                     /translation="MSPRHTALLILLFVVTSVAFIILDDQSALNPLKTGLRDLANPVT
                     GFVFDRTEKPKPPEGEWEAKYHDLEDKYAALDAEYQRTKMVADQVQALQAMLNLQQTN
                     PEVTFLPARVISVDPTGVNKIIVINRGSEDGVAVGMAVTDPNYYVGVVVKVDDTSAQV
                     RLAIDSTHTVGVELESSHATGVAYGMWQQGGRMEIRYVDRNVEIKPDEKVVTTCAPDI
                     RTANMPCGLIIGKASGQPTIDSQGDTQTVQVIPAADFEDLAFVTVITSQGTGQ"
     gene            2393..>3057
                     /locus_tag="VFI12_00090"
     CDS             2393..>3057
                     /locus_tag="VFI12_00090"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HET7054823.1"
                     /translation="MTRAIDLRLGLAVAAARVTTAGLRRTGRGGGTAAPGLIADFVDP
                     GILGKIGRRLERGVVVVAGTNGKTTTSRMIADVLEADGSRVVHNRSGSNLVRGVAAAF
                     AEQTSWTGQPDGDVGVVESDEAAFNAIVERLQPKVILLNNLFRDQLDRYGELDAIARK
                     WTAALSRLPATTTVVVNADDPTLAEISRDIPAKRITFGMDERRYLLAQLPHASDSAVC
                     RRCG"
BASE COUNT          542 a          988 c         1003 g          524 t
ORIGIN      
        1 tgcccacagg agacccgcat gaatcccgtc ctctggatcg tcttcggcgc gatcgccggc
       61 tggatcgccg gccggctcta tggcgacgac cggccggagg gctgcgtgac caacatcgtg
      121 cttggaatcg gcggagcgct cctcggcggc gcgatctata ccctgatcac cggccgggac
      181 ttcaccgccg gcttcaattt gccaagcttg atcgtcgcga tactcggcgc gctgctgctg
      241 attctcatcc tgcgacgcct tggtcggggc cggcactagg ccgcggccga ggtcggtcgc
      301 tgtgctatac tccggtgcgg tttccggccg cgttatgtcg cactaggtgg cgttaatcct
      361 gccctcctcc ggacagttcg ctcgaccctg caattcgcgg catatccgtc ggtaatcgcc
      421 ccgacgcttg acgcctggga ggtcccctct ctagtgattc gcccgctatc gacacttttc
      481 ggcttcttca gccgagatct tggcattgac ctgggcacag ccaacacgct ggtctatgta
      541 cgtggcaagg ggatcgtcat ctcagagccg tccgtcgtgg ccatcgatac caagacgaaa
      601 cgcgcacttg ccgtcggtgt cgaaggcaag aagatggttg gcaagacccc cgaaagcatc
      661 atcgccgtgc gtccgctcaa ggacggggtg attgccgact tcgacacggt cgagatgatg
      721 ttgcactact tcatccgcaa ggtgcacacg caacggatga tggcgccgca cccgcgcgtc
      781 gtggtcggca tcccgagcgg cgtgactgaa gtcgagaagc gcgcggccaa ggacgcggcc
      841 ctttcggccg gcgcccgcga ggcctacacg atcgaggagc caatggcggc cgcgatcggc
      901 gccggtttgc cgatcaacga gccggtcggc agcatgatcg tcgacatcgg cggcggcacc
      961 accgaagtgg ccgttatctc gctcggcggc atcgtcatca accactcgat ccgcgtcgcg
     1021 ggggacgaga tcgatgatgc gatcatccag tacgcccggc gggaccacaa cctggtcatc
     1081 ggcgaacgca cggcggagaa cgccaagatc gcggcggcat cggcttaccc gctcgaggaa
     1141 gagatccggg tcgtgcttcg cggtcgggac ttgctgaccg ggctgccgaa gtcggttgag
     1201 atcagctccg tcgagctgcg ggaggcgatt tcgccgcccg tcaacacgat cgtcgaactg
     1261 gtcaaaaccg cgattgagga gacgccaccg gagttggtgg cggacatcat ggagcagggc
     1321 attacgttgg ccggcggcgg cgccttgctg catgggttgg accggcggct tcagaatgag
     1381 ctgaagatgc cggtgatccg ggccgaggat ccgctgacct gcgtcgcgcg cggaacggga
     1441 cgggtggcgg aagcgcttca tctgtatgag cgcgcgttgg ccggtggccg ggaactgcgc
     1501 cgggccagtt aggggtcgtc ccgaccgacc cataggcact attcccgcgt ttgaggctga
     1561 agcatgtcat cgttgagtcc gcgacacacc gcgctgttga ttttgctgtt tgtcgtgacc
     1621 agcgttgcct tcatcatcct cgacgatcag tccgctctta acccgctcaa gaccggcttg
     1681 cgcgatctcg ctaacccggt cactggcttc gtcttcgacc gcaccgagaa gccgaagccg
     1741 cccgaaggcg agtgggaggc gaagtaccac gatcttgagg acaagtacgc cgccctcgat
     1801 gccgaatacc agcgcaccaa gatggtcgcc gaccaggtcc aggcgctcca ggcgatgctg
     1861 aacctgcaac agacgaaccc cgaggtcacc ttcctgcccg cccgcgtgat ctccgtcgac
     1921 ccgaccggcg tcaacaagat catcgtgatc aaccggggct cggaggacgg cgtcgcggtc
     1981 ggcatggcgg taacggatcc gaactactac gtcggtgtgg tggtcaaggt ggacgacact
     2041 tcggcccagg tacggctcgc gatcgactcg acccacacag ttggcgtcga gctggagagc
     2101 agccacgcga ctggcgtggc ctacggcatg tggcagcagg gtggccggat ggagatccgc
     2161 tacgtcgacc gcaatgtcga aatcaagccg gacgaaaagg tggttaccac ctgcgcgccg
     2221 gatatccgga ccgcgaacat gccgtgcgga ctgatcatcg gcaaggcctc gggccaaccc
     2281 accatcgaca gccagggcga cacgcaaacg gtgcaggtga tcccagcggc ggatttcgag
     2341 gatctggcgt tcgtgacggt gatcacctcg caaggcacgg ggcagtgaaa cggtgaccag
     2401 agcgattgat ctccgtctcg gattggccgt cgcggcggcg cgcgtgacga ccgccggcct
     2461 gcgccgcacc ggtcgcgggg gtgggacagc cgcgccggga ctgatcgccg atttcgtcga
     2521 tcccggcatc ctgggcaaga tcggccgccg cctggagcgc ggggtcgtgg tcgtggccgg
     2581 cacgaacggc aagacgacca cctcccggat gatcgccgac gtgctcgagg ccgatggctc
     2641 ccgcgtggtc cacaaccgct caggctcgaa cctcgtccgg ggcgtcgcgg ccgcatttgc
     2701 cgagcagacc tcgtggaccg ggcagccgga cggcgacgtc ggcgttgtcg aatcggacga
     2761 ggccgccttc aacgcgatcg tagagcggct ccagccgaag gtgatcctgc tcaacaacct
     2821 cttccgcgac caactcgacc gctacggcga gctggatgcg atcgctcgca aatggacggc
     2881 ggcgctgtcg cggttgccag ccacgacgac cgtagtcgtc aacgcggacg acccgaccct
     2941 ggccgagatc tcgcgcgaca tcccggcgaa acgcatcacc ttcgggatgg acgagcgccg
     3001 gtatctcctg gcgcaactgc cgcatgcctc cgattcggcc gtttgtcgac gctgcgg
//
LOCUS       DASSGX010000006         2263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1524609, whole genome shotgun sequence.
ACCESSION   DASSGX010000006 DASSGX010000000
VERSION     DASSGX010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1524609"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2202
                     /locus_tag="VFI12_00095"
     CDS             <1..2202
                     /locus_tag="VFI12_00095"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7054824.1"
                     /translation="VETGWKQVGSLYLAQTNDRLIQNRRAAAMARLFGIESHEISMDE
                     VRGIWPMMRTDDLVGALLIPADGRTEPASTARALATGAKLHGATVFEGVRVTELLHDG
                     RRATGVRTTTGDISAETVLLCGGMWMRQFGLQHGIDLPLYPVEHHYVVTNPVPGLHEM
                     MPCTRDHDGALYVRSEGTQMVIGAFQPGAKPWAVDQVPADFTFDLLEPDWVSFGTAIR
                     AANQRFPGFDEFGFDRFVNGPESFTPDSHAIVGPVVGWDQLYVCAGFNSFGIAGAGGM
                     GKAIAEWIIAGEEPMDLWEVDSRRFAGYQNEPRYLMDRVSEVLGHHYELAWPTAEFHS
                     GRGLKKSPLHERLAAAGACFGQRMGWERPLWFGEPGTEPVFDQSWGRAPWFDNWAREH
                     RAAREAVALFDQSSFGKLRVTGRDALQLLNQVSANEIDVSPGRLVYTAWLNRHGRFVS
                     DLTVVRHGPDEFSVMTAAVQPVHDRDWLERQARAIGADVCIEDVTDQTTVIGVMGPNS
                     RALLNRVTDADLSNDAFPFASVRSIEIGGGPVLAQRVTFVGELGWELHIPAAGAVTVF
                     DALVEAGSDLGLTLAGTMAMNSLRMEKSYVSWSHDVSRDDTPTEAELTFAVAWDKPGG
                     FLGRDALREQRASGIHRRLVTFLLDDSEALLWGHEPILRDGVPVGFTTSGSYAHTLSA
                     AIGMGYVRSNGPITREWVEAGRYEIEIAGTRYAAKPHWRASYDPSRSRILA"
BASE COUNT          353 a          747 c          792 g          371 t
ORIGIN      
        1 gtcgagaccg gctggaaaca ggtcggctcg ctctaccttg cacagacaaa cgaccgcctg
       61 atccagaacc gacgcgcggc ggcgatggcc cgcctcttcg gcatcgagtc gcacgagatc
      121 tcgatggacg aggttcgcgg gatctggccg atgatgcgga ccgacgacct cgtcggcgcg
      181 ctcctgatcc ccgcggacgg caggaccgag ccggcgtcga cggcccgggc attggccacc
      241 ggggcgaagc tgcacggcgc gacggtgttc gagggcgtcc gcgtcaccga actgctccac
      301 gacggccggc gcgcgaccgg cgttcgcacg accaccggcg acatctcggc tgagacggtc
      361 ctgctctgcg gcggaatgtg gatgcgccag ttcgggctgc agcacgggat cgatctgccg
      421 ctctacccgg tcgagcacca ctacgtcgtc accaacccgg tccccgggct gcacgagatg
      481 atgccctgca cgcgtgacca cgacggcgcg ctgtatgtca ggtccgaggg gacgcagatg
      541 gtgatcggcg cgttccagcc gggcgccaag ccgtgggcgg tcgaccaggt gccggccgat
      601 ttcaccttcg acctgctgga gccggactgg gtgtcgttcg gcacagcgat ccgcgcggcg
      661 aaccagcgct tcccgggctt cgacgagttc ggcttcgacc gcttcgtgaa cggaccggag
      721 agcttcaccc cggacagcca cgcgatcgtc gggccggtcg tcggttggga ccaactctac
      781 gtctgcgccg ggttcaactc gttcgggatc gccggcgcgg gcgggatggg caaggcaatc
      841 gccgagtgga tcatcgccgg cgaggagccg atggacctct gggaggtcga cagccgccgc
      901 ttcgccggat atcagaacga gccgcgctac ctgatggatc gcgtctcgga ggtcctcggc
      961 caccactacg agctcgcctg gccgacggcg gagttccact ccgggcgagg gctgaagaag
     1021 tcgccacttc acgagcgatt ggcggccgcc ggcgcgtgct ttgggcagcg gatgggctgg
     1081 gagcggccac tctggttcgg cgagccggga actgagccgg tattcgacca gtcgtggggc
     1141 cgggcgccgt ggttcgacaa ctgggcgcgg gagcaccggg cggcgcgaga ggcggtcgcg
     1201 ctcttcgacc aatcgtcctt cggcaagctg cgcgtgaccg gtcgtgacgc gctccaactg
     1261 ttgaaccagg tctcggcgaa cgagatcgat gtatcgccag gtcgcctcgt ctataccgcc
     1321 tggctgaacc gccacggccg cttcgtcagt gatttgacgg tcgtgcgaca cggtccggac
     1381 gagttctcgg tcatgaccgc cgccgtacag ccagtccacg accgcgattg gctcgagcgg
     1441 caggcccggg cgataggcgc ggacgtctgc atcgaagatg tcacggacca aacgaccgtg
     1501 atcggcgtga tggggccgaa ctcgcgcgcg ttgttgaatc gcgtgaccga tgcggatctg
     1561 tcgaacgacg catttccgtt cgcttcggtt cggtcgatcg agattggggg aggccccgtc
     1621 cttgcccagc gggtcacctt cgtcggcgag cttggctggg agctgcacat cccggctgcc
     1681 ggcgccgtca cggttttcga tgcgttggtt gaggctgggt ccgacctcgg gctgacgctc
     1741 gccggcacca tggcgatgaa ctcgctgcgg atggagaaga gctacgtctc gtggagccat
     1801 gacgtctccc gggacgacac gccaaccgag gccgaactca ccttcgcggt cgcgtgggac
     1861 aagccgggtg gcttcctcgg tcgcgatgcc ctgcgggagc agcgcgcatc gggcatccac
     1921 cgccgcctgg tgacattcct gctcgacgat tcggaggcgc tgctctgggg tcacgagccg
     1981 atccttcggg atggcgttcc ggtcggattc acgacctccg gcagctacgc ccacacgctc
     2041 agtgcggcga tcgggatggg atacgtgcgg tcgaacgggc cgatcacccg cgagtgggtc
     2101 gaagccggcc gctacgagat cgagatcgcc ggcacgcgct acgccgccaa gccgcactgg
     2161 cgcgcctcat atgacccgtc gcggagccgc atcctcgcgt agcgcagggt gtgaaccctg
     2221 ccgcgcgacc gacgacatgt ttgggacgca ccggcctgtc tcc
//
LOCUS       DASSGX010000007         3049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1307113, whole genome shotgun sequence.
ACCESSION   DASSGX010000007 DASSGX010000000
VERSION     DASSGX010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3049
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307113"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(122..877)
                     /locus_tag="VFI12_00100"
     CDS             complement(122..877)
                     /locus_tag="VFI12_00100"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET7054825.1"
                     /translation="MTAPDPLGKEAIEHGTIRGLPRRIVRRLILGLFFPLTRMRLKQL
                     ERVPKEGGVLVVANHIHNADPILLNAAYPRPIHFMAKKEVFDVPVLPWILRWVGAFPV
                     DRGKSDRGAIRRALAALNCGIAVAMFPEGTRSRVFALQKAHPGAGLLALTADVPVQPV
                     AITGTERLPFNGAKGSAAGELRREPGHPGVQILFGEPFRIPREVDGRRVTAEEATEIM
                     MREIARLLPEDYRGVYTDPPTSETPKRPADNAL"
     gene            complement(874..1551)
                     /gene="cmk"
                     /locus_tag="VFI12_00105"
     CDS             complement(874..1551)
                     /gene="cmk"
                     /locus_tag="VFI12_00105"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HET7054826.1"
                     /translation="MVALDGPAASGKTTVAAHLADRLGAVFLDTGLLYRAATLMAQRM
                     GLTAADELRISMLIDGGAVSIRPATVADGRRLDVALNGEDVTTMLRAPEIDAAVSAIS
                     ALPSVRAALMPLQRSFARDQRVIMAGRDIASVIFPDAAVKVYLDASLEERARRRWLEL
                     VVNAPMLTQNEVESELRRRDEIDSTRETAPLQVAEEAVIVQTDFKSIQQVVNEVAAIV
                     ERAWGVA"
     gene            complement(1466..2572)
                     /locus_tag="VFI12_00110"
     CDS             complement(1466..2572)
                     /locus_tag="VFI12_00110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HET7054827.1"
                     /translation="MERLQRILASRGVASRRAAEKLIQEGRVSVNGVRVTELGTKADP
                     RRDEIRVDNQVILAQPLRYLILNKPSGYITTVKDERDRWTVMDLINVPERVFPVGRLD
                     RDTEGLLLFTNDGDVANRVMHPRYGLAKEYQVLTRTRPTPQQLERIRRGIVIDGRRII
                     PQEVRLLRESADGIIITITVQEGIFHLVRRIMDVAGIEVERLRRARIGPLSISGLRIG
                     EWRDLTTGEQGTLFEALRLGEDQAAREADRPIRIRSSGAQPPANPLWKRPKRPGDEPE
                     PERKPNVPRYRKPSRTEREAAERGEPIAERQVRPESERDEQPTRERSRRPADRTERPA
                     RPERPQRGRPRRAGGEREDNRRGSSGRPPRRRVP"
     gene            complement(2607..>3049)
                     /locus_tag="VFI12_00115"
     CDS             complement(2607..>3049)
                     /locus_tag="VFI12_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7054828.1"
                     /translation="ATQGVVGLKTGSTEQAGGNLVLAKRERGGNLMIAVVLGSDLSYD
                     AQGMVEVDSRWDDMQAILGSVEGTFAWLNPESNNDMPGLMNEMAAWQVTLKDDSGILV
                     DRTQRSDVRYQLELMPQGPAKSDAGRVLFFAGPDLIAERPLIFR"
BASE COUNT          454 a         1043 c         1018 g          534 t
ORIGIN      
        1 cgatcgtccc tgacaggaac ggggttcgct ggggagtcgc ggccgtgccc ccgcgcggcc
       61 atacccgttc ggtatcaggc aatgggccgg tcgtgcaggg tgggtttgcg gtagcaaagt
      121 cctacagtgc gttgtccgca ggtcgtttcg gcgtctcact cgtcggcggg tcggtgtaga
      181 cgccgcggta gtcctcgggg aggaggcgcg cgatctcccg catcatgatt tcggtcgcct
      241 cctcggcggt gacgcggcgg ccgtcgacct cgcgcgggat ccggaacggc tcgccgaaga
      301 ggatctggac acccgggtgg ccgggctcgc gcctcagctc gccggccgcg ctgcccttcg
      361 cgccgttgaa cggcaatcgc tcggtgccgg tgatcgcgac gggctggacc ggcacgtcgg
      421 ccgtcagcgc cagcaagcct gcccccgggt gcgccttctg cagcgcgaac acgcgactcc
      481 gcgtcccctc cgggaacatg gcaaccgcga tgccgcaatt cagcgccgcc agtgcccgcc
      541 tgatcgcccc acggtcgctc ttgccgcgat cgaccggaaa cgccccgacc cagcgcagga
      601 tccagggcaa aaccggcacg tcgaagactt ccttcttcgc catgaagtgg atcgggcgcg
      661 ggtacgcagc gttgagcagg atcgggtcgg cgttatgaat gtggttcgcg accacgagga
      721 caccgccctc cttcggcacg cgctcgagct gcttgagacg catccgggtc agcgggaaga
      781 agagtccgag gatcagtcgc cgaacgatcc ggcgtggcag gccccggatc gtaccgtgct
      841 cgatcgcttc cttgccaagc gggtcaggtg ccgtcatgcg acgccccacg cgcgctcaac
      901 gatcgccgcg acctcgttga ccacctgctg gatcgacttg aagtcggtct ggacgatcac
      961 tgcctcctcc gcaacctgga gcggcgcggt ctcgcgggtg ctgtcgatct catcgcgtcg
     1021 tcgcagttcg gattccactt cgttctgggt cagcatcggc gcgttgacaa ccagctccag
     1081 ccagcggcgc cgggcacgct cttcgaggct ggcgtcgagg tagaccttga ccgccgcgtc
     1141 ggggaagatg accgaggcga tgtcccggcc ggccatgatc acccgctgat ccctggcaaa
     1201 cgagcgctgg agcggcatca gcgccgcgcg gacggacggg agcgcggata tcgccgagac
     1261 tgcggcatca atctccggcg cgcgaagcat ggtcgtaaca tcctcgccat tgagcgcgac
     1321 atccaggcgg cggccatccg cgacggtggc cggccggatc gagactgctc cgccgtcgat
     1381 cagcattgaa atccgcagct cgtccgcggc ggtcaggccc atccgctggg ccatcagcgt
     1441 ggcagcgcga taaaggagtc cggtatcaag gaacacggcg ccgaggcggt cggccagatg
     1501 agccgcgacg gttgtcttcc cgctcgccgc cggcccgtcg agggcgacca cgttgcggcc
     1561 gttccggtcg tgctggtcgc tccgtccggt cggctggccg ccggctgcgc tctcgtgtcg
     1621 gttgctcatc tcgctcactt tcggggcgca cctgccgctc cgcgatcggc tcgccgcgct
     1681 cggccgcttc ccgctcggtc cggctcggct tgcgatagcg cgggacattc ggttttcgtt
     1741 ccggttccgg ctcgtccccg ggccgtttcg gtcgcttcca gagcggattg gcgggaggct
     1801 gtgcgccgct ggaccgaatc cgaatcggcc gatcggcctc gcgcgctgcc tggtcctccc
     1861 cgagacggag tgcttcgaat agcgtcccct gctcgcccgt cgtcaaatct cgccattcgc
     1921 cgatccgcaa tccggaaatc gagagcgggc cgatccgcgc tcgccgcaac cgctcgacct
     1981 cgatcccggc gacatccatg atccgccgga ccaggtgaaa gatgccctcc tggacggtga
     2041 tcgtgatgat gatgccatcg gccgactccc ggagcagtcg aacttcctgg ggaatgatcc
     2101 gccggccgtc gatcacgatc ccgcggcgga tccgctcgag ctgctgcggc gtcggccggg
     2161 tccgggtcag gacctgatac tccttggcca gcccatagcg cgggtgcatc acccggttcg
     2221 ccacgtcgcc gtcgttcgtg aagagcagca gcccctcggt gtcgcggtcg agccggccga
     2281 ccgggaatac ccgttcgggc acattgatca ggtccattac ggtccagcga tcgcgctcgt
     2341 ccttgacggt ggtgatgtag ccgctcggct tgttgaggat caggtaccgg agcggctggg
     2401 cgaggatgac ctggttgtcg acccggatct cgtcgcgccg ggggtcggct ttcgtgccaa
     2461 gctcggtcac gcgcacgccg ttcaccgaga cgcggccctc ctggatcagt ttctctgcgg
     2521 cgcggcgcga tgcgacgccc cgcgaggcga ggatgcgctg caatcgttcc aaaggggtgc
     2581 tccccggcga gtcgtaattc gcgatgctag cggaaaatca gtgggcgctc ggcgatcagg
     2641 tccggcccgg cgaagaatag cacgcgcccg gcatcgctct tcgccggacc ctgtggcata
     2701 agctccagct ggtagcgaac atcagaccgc tgggtgcggt cgacgaggat gccagagtcg
     2761 tccttaagcg tgacctgcca cgcagccatc tcgttcatca gacccggcat gtcattgttc
     2821 gactcgggat taagccaggc gaaggtgccc tcgaccgagc cgagaatcgc ctgcatatcg
     2881 tcccagcgac tgtcgacctc caccatgccc tgcgcgtcgt agctgaggtc gctgccgagg
     2941 acgacggcga tcatcaggtt gccgccgcgc tctcgcttgg cgaggaccag gttgccgccc
     3001 gcctgctcgg tcgaaccggt cttcaggccg acgacgccct gagtggcgt
//
LOCUS       DASSGX010000008         2170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1289, whole genome shotgun sequence.
ACCESSION   DASSGX010000008 DASSGX010000000
VERSION     DASSGX010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1289"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VFI12_00120"
     CDS             complement(<1..263)
                     /locus_tag="VFI12_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7054829.1"
                     /translation="MVSTPLRSTKDLIDRKKVQELTDLEERRLNANTEVSTQMYERAK
                     THMVGGVPSSYQSRDPWPIYLTRGKGSKVWDVDDTEYVDFHNG"
     gene            complement(564..1115)
                     /locus_tag="VFI12_00125"
     CDS             complement(564..1115)
                     /locus_tag="VFI12_00125"
                     /inference="COORDINATES: protein motif:HMM:NF040769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HET7054830.1"
                     /translation="MTTTNLHTAPDLAFTNADGAEIHLSDFWESAPNGIVLVFLRHFG
                     CIFCRDHSTQLREHYAEFQDRGWQVVAIGQGTPARAAKFRADNDLPYPILGDRSLDSY
                     RAYGLTHGGMGGLLQPKSWPAGVRSMLHGHRPGRPDGGDINQNPGTFLIDGSGRIHRA
                     RIGQHAGDFPSAREILGWIDSQN"
     gene            complement(1112..1567)
                     /gene="rpiB"
                     /locus_tag="VFI12_00130"
     CDS             complement(1112..1567)
                     /gene="rpiB"
                     /locus_tag="VFI12_00130"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HET7054831.1"
                     /translation="MRLAIGGDHAGFPLKRPVIDALRQWGHEVVDLGTHSTEPVDFPD
                     ITQAVCAQLIDGQADRAILVCGTGVGACIAANKIPGIRAALCHDVYSAHQSVEHDDVN
                     VLCLGAQIVGLPLALDLIRTFLDARFSDEEHFRRRVRKLAELERRNSTR"
     gene            1639..>2170
                     /gene="tsaA"
                     /locus_tag="VFI12_00135"
     CDS             1639..>2170
                     /gene="tsaA"
                     /locus_tag="VFI12_00135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HET7054832.1"
                     /translation="MAEHKRWFRAQQIGVVHRADQAGPNEFLDPAAESVIRIDPRWEA
                     GLAGIEEFSHLVVLFQLDRAERRRTAGEPMRPEGRAEVPAVGFFATRTPRRPNPIGIG
                     CPKLIRRDGGDLHMSGLDAWDGTPVLDIKGYYPRDEVRPDATVPEWLTALWRQHDSER
                     APGSVSSLPQPGTVVAE"
BASE COUNT          363 a          651 c          773 g          383 t
ORIGIN      
        1 aagccgttgt ggaagtcgac gtactccgta tcgtcaacgt cccaaacctt cgagcctttg
       61 ccgcgcgtaa ggtagatcgg ccatggatcg cgtgactggt aggacgacgg cacgccgccg
      121 accatgtgcg tcttggcgcg ctcatacatc tgggtcgaga cctcggtgtt ggcgttgagg
      181 cgccgctcct cgaggtcggt taattcctga acttttttgc gatcaatcag gtctttggtt
      241 gagcgcagcg gtgtgctaac cacgaaaaat cactcctggt atgccaataa cggtcgaggg
      301 ttctactgcc gggttggacg tggaggagga cggtcgaccg gcagggcgcg ccaatccccg
      361 aacgacatct cgatggaggc gatggcaagg tccaccagat ggcgatagag atcgaggaat
      421 cggtttgctt ggtgcgcctg ggacagccgt tgcgctgtca tgtatcagcc cgtttcggtc
      481 aggttgccgg acgcgatagc gcctccagcg gcaatctcgg cttgatgttg ggactgatcc
      541 taccagagcc gcgcggcatt tgcttagttc tgcgaatcga tccagcccag aatctcccgc
      601 gcggacggga aatctccggc atgctggccg atccgggcgc ggtggatgcg tccggagccg
      661 tcgatcagga aggtgcccgg gttctggttg atgtcgccgc catccggccg tcccgggcga
      721 tggccatgga gcatcgagcg gacgccggcc ggccatgatt tcggctggag caggccgccc
      781 atgccgccgt gggtcaggcc gtacgcgcgg taggaatcga gcgaccggtc gccgaggata
      841 gggtagggca ggtcgttgtc ggctcggaac ttcgctgcgc gggccggcgt gccctgcccg
      901 atcgcgacca cttgccagcc gcgatcctgg aattcggcgt aatgctcccg gagctgggtg
      961 gaatggtcgc ggcagaagat gcacccgaag tggcgcagga agacgagcac gatcccgttc
     1021 ggcgccgatt cccagaagtc ggagaggtga atctcggcgc cgtcggcatt ggtgaaggcg
     1081 aggtcgggcg cggtgtgcag gttggtcgtg gtcatcgggt ggagttccgt cgctcgagct
     1141 cggcgagctt ccggacacga cggcggaaat gctcctcgtc gctgaagcgg gcatcgagga
     1201 acgtacgaat caggtcgagc gcgagcggca gcccgacgat ctgcgcgccg aggcagagca
     1261 cgttgacgtc gtcatgttcg acggattgat gcgcggagta gacgtcgtga caaagggcgg
     1321 cgcggatgcc cgggatcttg ttggcggcga tgcaggcgcc gacgcccgtg ccgcaaacaa
     1381 ggatcgcgcg gtccgcctgc ccgtcgatca gttgcgcgca aacggcttgg gtgatgtccg
     1441 ggaaatcgac cggctccgtc gaatgcgtgc cgaggtcgac gacctcatgt ccccattgcc
     1501 ggagcgcgtc gatgactggt ctcttcagtg gaaacccggc gtggtcgccg ccgattgcca
     1561 accgcatgcc cctgcttgcc tccgaaacga cgtgtatccc tgctgatgag catacttccc
     1621 gcgcggaaag gacggagcat ggccgagcac aagcgctggt ttcgggcgca acagatcggg
     1681 gtggttcatc gcgcggatca ggccggcccg aatgagtttc ttgatccggc ggcggagtcg
     1741 gtgatccgga tcgacccgcg ctgggaggct gggctggccg ggatcgagga gtttagccac
     1801 ctcgtcgtcc tcttccagct cgaccgggcc gaacggcggc gcaccgccgg cgaaccgatg
     1861 cggccggagg ggcgggccga ggtgcccgcg gtcggcttct tcgcgacccg cacgccgcgc
     1921 cggccgaacc cgatcggaat cggctgcccg aagctgatcc gcagggacgg cggcgacctg
     1981 cacatgtcgg ggctggacgc ctgggacggc acgccggtgc tcgacatcaa gggctactac
     2041 ccgcgagatg aagtgcgccc ggacgccacc gtgccggagt ggttgacggc gctctggcgg
     2101 cagcacgact cggagcgggc gcctggatcg gtctcgtcgc tgccacagcc tggaacggtc
     2161 gtggccgagc
//
LOCUS       DASSGX010000009         2945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1198575, whole genome shotgun sequence.
ACCESSION   DASSGX010000009 DASSGX010000000
VERSION     DASSGX010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1198575"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..1325
                     /locus_tag="VFI12_00140"
     CDS             45..1325
                     /locus_tag="VFI12_00140"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HET7054833.1"
                     /translation="MTMDRARSFLTREVALRLLASIALALILWALVTLRQDPETSRAF
                     NDIPVQALSLDASLVIVNEVEPVRVELSGPRSDVVPIDAGALVANIDFSTVEQPGVYE
                     LPVSVPTPKGIWSSEVSPATASVVIERSESEPFRLAPVVTDLDENSLRTVTVSPEVDQ
                     VTVTGPTSLIDSIHEVVLPVQVSGGTQTFQDVYVPEARDQSGEVIEGVSIEPAAVNAT
                     VRVSARGKSVAVLANITGTPATGFEVADRTVNPQFVIVDGEEGALDSLVALSTEPIDV
                     SGADASFNRKVGIVGLPDGVQILQPSDAKVEVLVQITQRGVHQSLPSQQVTVVGVGPG
                     LVASVSPDEITIEVVAPDDALASLDSKTLQVVVDASGLTAGTYAVQPSVIMPPRVQWV
                     TTFPAEVTLTLSPAPGTAESSPNPTASGTPESAP"
     gene            complement(1364..2041)
                     /locus_tag="VFI12_00145"
     CDS             complement(1364..2041)
                     /locus_tag="VFI12_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7054834.1"
                     /translation="MAGVPGAIIIGGSMRPTNIQRTTCRILIADDESLIRLDLKEMLT
                     HLGYDVVAEVGDGGAAIDLSRKLRPDLVIMDIKMPEVDGIAAAEILTREQIAPVVLLT
                     AYSDQALVERARDAGVVGYVVKPFREAELLPVIELSLTRFAEFTALRKEVGDLRDALE
                     TRKVVERAKGVLMEVHGLPEADAFRRIRKTSMDSRKSMKEVAEAILLTYEMDISATPA
                     GDATDGM"
     gene            complement(2103..2176)
                     /locus_tag="VFI12_00150"
     tRNA            complement(2103..2176)
                     /locus_tag="VFI12_00150"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2140..2142),aa:Pro,seq:ggg)
     gene            complement(2339..2719)
                     /gene="rplL"
                     /locus_tag="VFI12_00155"
     CDS             complement(2339..2719)
                     /gene="rplL"
                     /locus_tag="VFI12_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017150119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HET7054835.1"
                     /translation="MSNAALIEQLEKMTVLEINDLVKELEDRWGVSAAAPVAVGGAVA
                     AGGGAEAAPAEEQTEFDVILTDIGANKIQVIKAVRELTSLGLKEAKDLVEAAPKAVKE
                     AVSKEEAQAAKAKLEEAGAKADVK"
BASE COUNT          547 a          912 c          903 g          583 t
ORIGIN      
        1 cgggccaagg aatccgcgca ttccggagct cagggaggcg tgtgatgaca atggaccgcg
       61 cccgttcgtt cctgactcga gaagttgcgc tcaggctgtt ggcgtcgatc gcgctcgcgc
      121 tgattctctg ggcactcgtc acgctgcggc aggatcctga aacctcccga gcgttcaacg
      181 acattccggt acaagcattg tcactcgacg cttcattggt catcgtcaat gaggtcgaac
      241 ccgtgcgagt tgagctgtcg gggccccggt ccgacgtcgt cccgatcgac gcgggcgcat
      301 tggtcgccaa tatcgatttc agcacagtgg aacaacctgg cgtctacgaa ctgcccgttt
      361 cggtgccgac gcccaaggga atctggagtt cggaagtttc accggcgacc gcctcagtcg
      421 tcatcgagcg gtccgaatcg gaaccattcc gcctggcgcc ggtagtcacc gacctcgatg
      481 aaaacagctt gcggacggtg accgtctctc ccgaggtgga ccaggtcacc gtcaccgggc
      541 caacctcgct gatcgattcg atccacgagg tggtcttgcc cgtgcaggta tccggcggca
      601 cgcagacctt ccaggatgtc tatgtcccag aagcgcgcga tcagtccggc gaagtgatcg
      661 aaggcgtgtc gatcgagccc gctgctgtga atgccactgt ccgagtgtcg gcgcgcggaa
      721 agagcgttgc ggtgctggcg aacattaccg gcacgccagc aacaggcttc gaggtggcgg
      781 atcgcacggt caatccccag ttcgtgatcg tcgatggcga agaaggtgcg ctcgactcgc
      841 tcgtcgcact atcgaccgag ccaatcgatg tgagtggcgc ggatgcgagc ttcaatcgaa
      901 aagtcggcat cgttgggctg ccggatggcg ttcagatcct gcaaccgtcg gacgcgaagg
      961 tcgaggtgct cgtgcagatc acccagcgcg gggtgcatca atcgttgccc tctcagcagg
     1021 tgacagtggt tggagttggc ccgggacttg tcgcgtctgt gtcaccggac gagattacga
     1081 tcgaggtcgt tgcgcccgac gatgcgctgg ccagtctcga ttcgaaaacg ctgcaggtgg
     1141 tggtggacgc cagcgggctg acagccggca catacgcggt gcagccgtcg gtcatcatgc
     1201 ctccgcgcgt ccaatgggta acgaccttcc cggcagaggt aacgttgact ctttcgccag
     1261 cgccgggtac ggccgagtcg tccccgaatc caacagcttc cggaacgccg gagagcgctc
     1321 cctgaggaag cgctcccgac aacgagagtt ctagactggg gttctacatt ccgtcggtgg
     1381 cgtcaccggc tggagtggcc gatatatcca tctcgtaggt cagcaggatt gcctcggcga
     1441 cttctttcat cgatttgcgc gagtccatgc tggttttacg gatgcggcga aaagcatcgg
     1501 cctccggaag cccatggacc tccatcaaaa cacccttggc ccgctcgact accttgcggg
     1561 tttcgagcgc atcccgtaga tcgccgactt cttttcgcaa cgcggtgaat tcagcaaacc
     1621 gggtgagcga aagctcgatc accggcagga gctcagcttc gcggaatggc ttgacgacat
     1681 atccaacgac accggcatcg cgcgcgcgct cgaccagcgc ttggtcggag taggccgtga
     1741 gcagcacgac tggtgcaatc tgctcgcgcg tcagaatctc ggcggctgcg atgccgtcga
     1801 cttcaggcat cttgatatcc atgatgacca gatcgggcct gagcttgcga cttaggtcga
     1861 tggccgcccc gccgtcgccg acttccgcga ccacgtcgta ccccaggtgg gtgagcatct
     1921 ctttcagatc gagccgtatg agcgattcat cgtcggcaat gagaatacgg caggttgttc
     1981 gttggatatt cgtcggtcgc atcgatccac cgatgatgat cgcaccaggc acgccggcca
     2041 ccgatggcca cgttggaggt ggaagccaaa gtcgtagggt tttcgccgca acagcgagac
     2101 ggtcggggtg agaggattcg aacctccgac cactggtacc ccataccagt gcgctaccgg
     2161 actgcgccac accccgtgac gcaccattat aacgcggacg cgccaattca cctactagcc
     2221 tcgagcggga ctgagctgaa tcgtagccgc caattcgaat cgtcagccac aggggcgtcc
     2281 cggagacgac aacgggacgg ccgcattggc cgtcccattt tcgctgctcg caaggaggtt
     2341 acttgacgtc ggctttggcg ccggcctctt ccagtttcgc cttggcggcc tgagcctctt
     2401 ccttggatac ggcttccttg accgccttcg gggcggcttc gaccagatcc ttggcttcct
     2461 tcagtcccag actggtgagc tcgcggacgg ctttgatgac ctgaatcttg ttcgcgccga
     2521 tatcggtcag gataacgtcg aattcggtct gctcctcggc cggtgcggcc tcggcgccac
     2581 caccggcggc gaccgcgccc ccgacagcga ccggggccgc ggcggaaacg ccccagcgat
     2641 cctccagctc cttgaccagg tcattgatct cgagaacggt catcttctcg agttgttcga
     2701 tcaatgcagc attgctcatt gttgactcct gttgttggga cgatccattc gccccaggct
     2761 ctgacgaaaa acgacaaaac gaatcgaact agtcggcggc catggcttca ctgccgagct
     2821 ggccggcacg agcgttgagc acgtacacgg cggaacggct cgggccgctg agcacggcca
     2881 gcgtgcgggc caacggcgat tgcagcagtc ccaggagctt gccttgaagc tgttccctgg
     2941 acggc
//
LOCUS       DASSGX010000010         2375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_219350, whole genome shotgun sequence.
ACCESSION   DASSGX010000010 DASSGX010000000
VERSION     DASSGX010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219350"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(262..489)
                     /locus_tag="VFI12_00160"
     CDS             complement(262..489)
                     /locus_tag="VFI12_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7054836.1"
                     /translation="MANRTKVTQQKRARERAKQERQQEKTRRREEAKTRKANEPHLSG
                     DEDPDIAGIIPGPQPSPWGDEDLIDTEKLEK"
     gene            666..2057
                     /locus_tag="VFI12_00165"
     CDS             666..2057
                     /locus_tag="VFI12_00165"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET7054837.1"
                     /translation="MQTPIPAHRREAVAQLKALLGSRASDVLSVREHHSHGESYHPPA
                     APDVVCFPSSLDEVVAIVRISEQCGIPVVPFGAGTSLEGHVQALRGGICIDCRELNRV
                     VRIGVDDLDATVEAGVTRLQLNKALRNTGLMFPIDPGADATIGGMTATRASGTTAVRY
                     GTMRENVLGVTAVLADGRVIRTGTRARKSSAGYDLTRLLVGSEGTLGIITDVTVRLHP
                     VPEAVAAAVCAFDSIKGAVDTVIATIQLGVPVARIELLDDVQMDAVNRYSKTNYAVAP
                     TLLFEFHGDSDRHVSDQAAAVEALARERGGREFDWATRPEDRERLWHARHTVHFAALG
                     LRPGCRSWATDVCVPISRLSDCILETKAEHADAPFPVCLVGHAGDGNFHVIYLLDPAS
                     EEERAVAQRLNDRMVARALSMGGTCSGEHGVGYGKMKYLEAEHGEGLGVMRAIKRALD
                     PDNRMNPGKIVDI"
     gene            complement(2088..>2375)
                     /locus_tag="VFI12_00170"
     CDS             complement(2088..>2375)
                     /locus_tag="VFI12_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7054838.1"
                     /translation="DLIEIVVDTRTLEGSLNMIGESGIEIGAAAGARLLERRGPRPDL
                     AADPALPDDTRIWAALQDISGGAWGGSVYDADAILRVIEAGKRAVNRSSPE"
BASE COUNT          390 a          771 c          777 g          437 t
ORIGIN      
        1 gcagaaatga gtatctgtat gcctaccgat ggggacgatt gatgcggaag aactgatctc
       61 tcggcgcgac aggcagtaga cagcaggcgc caggcgtgga attccggtct cctccgggcc
      121 ggggtaccgt ttcagcttgc agcgctggtc tggtgaaatt ctctcctgct gtctactggc
      181 tgtcgcgctc atctcaccag gcgcgaagtt cttcggggaa tcgcccgaac cgcgtcagcc
      241 ggacgctgtc gtcagaaacc tttacttctc cagcttttcg gtatcgatca gatcctcatc
      301 tccccacggg ctcggctgag gtcccgggat gatgccggcg atgtccggat cttcgtcgcc
      361 ggacagatgc ggctcgttcg ccttgcgagt cttggcttcc tcacgacgac gggtcttctc
      421 ctgctggcgc tcctgcttgg cgcgttcgcg cgcgcgcttc tgttgtgtca ccttcgtgcg
      481 gttcgccatg gatatctccc tatgtcagtc gcggcgtgcg cggtacagca aaaaggccgg
      541 ttggcgaccg gccctggcgg aatcgagggg ctatgcaggc tgtcgcctcg gtcatcaact
      601 tgattctagc tgatccgccc gtccggtggt ggcgtttctt ggaaaagagt acaaatgacc
      661 gatcaatgca gacaccgatc ccggcccatc gccgcgaagc ggtggcgcag ctcaaggcgc
      721 tgctcggctc gcgcgccagc gatgtcctct cggtacgcga acatcacagc cacggcgagt
      781 cctatcaccc accggcggcg cccgatgtcg tctgctttcc ctcatcgctc gacgaggtcg
      841 tcgcgatcgt ccgaatcagc gaacaatgcg gcatcccggt ggtgccgttt ggcgccggca
      901 cgtccctcga agggcacgtt caggcgctcc gcggcggcat ctgcatcgac tgccgcgagt
      961 tgaaccgggt cgtgcgaatt ggcgtggacg atctcgacgc caccgtcgaa gcgggcgtca
     1021 cgcgactgca actgaacaag gcgctgcgca ataccgggct gatgtttccc atcgatccgg
     1081 gcgcggacgc aacaatcggc ggcatgacgg cgacccgagc atcgggcacg accgccgtgc
     1141 gctacggcac gatgcgcgag aacgtcctcg gcgtgacggc agtgctcgcc gacggacgcg
     1201 tgattcgcac cggcacacgg gcgcgcaagt cgtctgccgg atacgatttg acccgcttgc
     1261 tggtcggttc cgaaggcacc ctcggcatca tcaccgacgt aacggtccgg ttgcaccctg
     1321 tgccggaggc ggttgcggcg gcagtgtgcg cattcgactc gatcaaaggg gcggtcgaca
     1381 cggtcatcgc cacgatccag ctcggggtgc cggtcgctcg tatcgaactg ctcgatgacg
     1441 tgcagatgga tgcggtgaac cgatactcga agaccaacta cgcggtcgcg ccgacgctgc
     1501 tgttcgagtt ccacggtgac tcggatcgac atgtctccga tcaggcagcc gcggtcgagg
     1561 cgctcgcgcg cgagcgaggc ggccgggaat tcgactgggc gacgcgcccc gaggatcgcg
     1621 aacggctgtg gcacgcccgg cacaccgtgc attttgccgc gctcggactg cggccgggat
     1681 gccgctcctg ggcgaccgac gtctgcgtgc cgatctcgcg cttgtcggat tgcatcctcg
     1741 aaaccaaggc cgagcacgcc gacgcgccgt ttccggtgtg cctcgtggga cacgcggggg
     1801 acggcaattt tcacgtgatt tatctcctcg atcccgccag cgaggaggaa cgggcggtgg
     1861 cgcagcggct gaacgaccgg atggtcgcgc gggcgctgtc gatgggcggc acgtgcagcg
     1921 gcgagcacgg cgtcggctac ggcaagatga aatatctgga ggccgagcac ggcgagggat
     1981 tgggcgtcat gcgggcgatc aagcgcgcgc tcgatcccga taaccggatg aatcccggca
     2041 agatcgtgga catctgatcg atcgatcaga cctccgcgcc ggtgcgctta ctccggggat
     2101 gaccggttga cggcgcgctt ccctgcctcg atcacccgca ggatcgcatc ggcatcgtag
     2161 accgatccgc cccacgcacc gccgctgatg tcctgcagcg ccgcccagat gcgcgtgtcg
     2221 tcgggcagcg ccggatccgc ggcgagatcc ggcctcggtc cgcgccgctc cagcaggcgc
     2281 gctcctgcgg cagcgccgat ctcgatcccg gactcaccga tcatgttcaa cgatccctcg
     2341 agcgttcggg tatcgacgac gatctcgatg aggtc
//
LOCUS       DASSGX010000011         2231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_110490, whole genome shotgun sequence.
ACCESSION   DASSGX010000011 DASSGX010000000
VERSION     DASSGX010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110490"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..576)
                     /locus_tag="VFI12_00175"
     CDS             complement(121..576)
                     /locus_tag="VFI12_00175"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7054839.1"
                     /translation="MNGVKKVDHIAIAVKSIAESRPWFESVYGARYLGQKENVDQQYI
                     VAYFLMGESLITMLEGTTPESFVTQHIEKRGEGIQHMGVEVDDLDAFLANAESLGARV
                     SNQRTIEGIRKEALISPKSAFGIILQPIEWLGDLKTEPHHERIIKAGGL"
     gene            839..2140
                     /locus_tag="VFI12_00180"
     CDS             839..2140
                     /locus_tag="VFI12_00180"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HET7054840.1"
                     /translation="MAVRVLCTGDIHIGRSASKVPDAPRCSQAWTAIVDLAIAERVDL
                     LAISGDIVDKENKSYEAIGPLQDGLRRLNEAGIDTVAIAGNHDHDVLPRLSGFAGTDR
                     FHLLGSDGEWERWTLLRNGTPRLQVCGWSFPKEHVRENPLHTFMDGADDGVPVLGLLH
                     AEVGNTRSLYAPVSFDDLRAHRVDFWLLGHIHAPRQFPDTNGCRALYPGSPFAMDPGE
                     PDAHGVWMLTIEPGRPMVPEMIAISPVRYSQLNVDVTGIDDRDGFLERLTSELLATGR
                     AGDGDHSAAHLLAVSCRIRLTGSSTAHRLIPIWATQARQDIDPYPAGRVSVFIDEITT
                     DVRPHIPLDQLVRMNDALGATARLIAAMDATVPEPPYADLIRRTAAELSSVHTHPGYA
                     AIRSGDQTDAPGRLTEDDARDLLRTEGWRLLSALLDQRDVA"
BASE COUNT          379 a          705 c          694 g          453 t
ORIGIN      
        1 gcagatcgcc tcttcgaggc tggggtgcgc ggcataggtc gcattgctag acgacgctac
       61 tgttcttctt cgcgccctgc ttctcgactg ctcgccttct cgactgctcg ccttctcgac
      121 tcacaagccg ccggctttga tgatgcgctc gtggtggggc tcggtcttga ggtcgccgag
      181 ccattcgatg ggctggagga tgatgccgaa ggcgcttttt ggcgagatca gcgcctcctt
      241 gcggatgcct tcgatcgtgc gctgattgga gacgcgggcg ccgagcgatt cggcgttggc
      301 aagaaaggcg tcgagatcgt ccacttccac gcccatgtgc tggatgccct caccccgctt
      361 ttcgatgtgc tgggtgacga aggactccgg cgtggtgccc tcgagcatcg tgatcagcga
      421 ttcgcccatc aagaagtacg cgacgatgta ctgctggtcg acgttctcct tttggccgag
      481 atagcgcgcg ccgtagaccg actcgaacca gggccgagat tcggcgatgg acttaaccgc
      541 gatcgcaatg tgatcgacct tcttgacgcc attcatgcag gtagcactcc ccgagacgtg
      601 agacgtgaga cgtgagacgt gagggagttt agccgggtta cgcctggcga ctgttccggt
      661 tctcctgaac tccacaacca gtaacttatc tcttattgcg attcccatcc ctgcgtccgt
      721 atcgtgggtt cgaccccgcg tgtcgtgtcc gacgcagacc gcagcggccg ctcgcctgcg
      781 cgttcgtctg tgctcgcgct cctgtctcag cgggccactg ttggccaagg atgaggtaat
      841 ggcagttcgc gttctctgta ctggcgacat ccacatcggg cgaagcgcca gcaaggtccc
      901 cgacgcgccc cgttgctcgc aggcctggac ggcgatcgtt gatctcgcca tcgccgagcg
      961 ggtcgatctc ctcgcgatca gcggcgacat cgtcgacaag gagaacaagt cgtacgaggc
     1021 gatcgggcca ttgcaggacg ggctgcgccg gctgaatgag gccggtatag acacagtggc
     1081 gatcgcgggg aaccatgatc atgatgtgct ccctcgcctt tccggatttg ccgggaccga
     1141 ccggtttcac ctgcttggat cggacggtga gtgggagcgt tggacgctgt tgcgcaacgg
     1201 aactccgcgg ctgcaggttt gcggctggtc gttcccgaag gagcacgtcc gcgaaaaccc
     1261 actccatacc ttcatggacg gggcggacga cggcgtcccg gtactggggc ttctccatgc
     1321 cgaggtcggc aacacgcgga gcctctatgc gcccgtgtcc ttcgatgact tgcgggcgca
     1381 ccgcgtcgac ttctggttgc tcggccatat ccacgcgcca cggcaatttc ccgacacgaa
     1441 cggctgccgt gcgctgtatc ccggatctcc attcgcgatg gatcccggcg aacccgacgc
     1501 tcacggcgtc tggatgctga caattgagcc cggccggccc atggtgccgg aaatgatcgc
     1561 gatctccccg gttcgctact cccaactcaa cgtcgacgta accgggatcg acgacagaga
     1621 tggcttcctg gagcgtctga catccgaact tcttgccact gggagggctg gggacggcga
     1681 tcactcggcg gcacacttgt tggcggtcag ttgtcgaatt cgcctcaccg gcagcagtac
     1741 tgcgcaccgg ctgatcccga tctgggcaac gcaggcgcgc caagacattg atccctaccc
     1801 cgccgggagg gtgtccgtct tcatcgatga aatcacgacc gatgtccggc cgcacatccc
     1861 gctcgatcag ctcgtccgca tgaatgatgc gctcggggcg acagcccggc tgatcgccgc
     1921 aatggatgcg acggttccgg agccgccgta tgcggacctc atccgccgaa ccgccgccga
     1981 gttgagctcg gtccacacac accccggata tgcggcgatt cgttccggag accagactga
     2041 cgctccaggc cgccttaccg aggacgatgc gcgcgatctg ctgcgaaccg aaggatggcg
     2101 gctcctgtcc gcgttgctcg accagaggga tgttgcatga cccctgaaat gacgcagacc
     2161 agccggcttc cgctccttgt tgagcgagtc gccgttgagc ggctcgacgg cattgttgga
     2221 cggttcgaaa t
//
LOCUS       DASSGX010000012         2524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_655123, whole genome shotgun sequence.
ACCESSION   DASSGX010000012 DASSGX010000000
VERSION     DASSGX010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655123"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /locus_tag="VFI12_00185"
     CDS             <1..1065
                     /locus_tag="VFI12_00185"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7054841.1"
                     /translation="PVLAAAIDRHVVIAIREDPIQVGVRADFADIPGEFAHVLPDLAH
                     DWRNPGQSTASGDYLRGAIAALQTRGQAVRTGLELVVAGDLPYGVGISSSAALCVALV
                     LALARETPAPSEVVLIAQEAEHRAGSPCGTMDQSASVYGGLIRYEGADNSVRTIAADL
                     AGHQFVVVNSGVVRTLAGSAYPERVRETQAALAILQGRWREDLPALGAIASADLVAAL
                     AVLESAGAAKLVNRVRHVVTEGQRVRDAERAIAGSDWAEVGRLMTASGHSSASDYDMS
                     HPQVERVVSLCLDVPGVLGARMMGGGQGGSALVLARSESVDALRDRLDRDYFNELRNP
                     PGVRMIPCTFAPGAGPMEPA"
     gene            complement(1062..1388)
                     /locus_tag="VFI12_00190"
     CDS             complement(1062..1388)
                     /locus_tag="VFI12_00190"
                     /inference="COORDINATES: protein motif:HMM:NF025442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HET7054842.1"
                     /translation="MADRQLESVSIHASQRLAQRNLSTRDIEYVLNHGRRIRNGKAVF
                     ICLGRRDIPAADQRESSVTRLEGTVLVLDPMTGSHLTTAYRNRRSGIRDVKRKQKHAL
                     PSRYSR"
     gene            complement(1577..>2524)
                     /gene="moaA"
                     /locus_tag="VFI12_00195"
     CDS             complement(1577..>2524)
                     /gene="moaA"
                     /locus_tag="VFI12_00195"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HET7054843.1"
                     /translation="YLRISLTDRCNFRCVYCMPEMGMKFQPREEILTDDELIKVVGAF
                     AGLGFTKFRLTGGEPTIRPHLVDIVRAIKQFPGVEEVTMTTNALLLGRMADGLADAGL
                     DRINVSLDTLNPDRFKAMTRGGRFDMVWEGLLAAERAGITPIKINTVVVRGQNEHEVD
                     ELARLTIDRPWQMRFLEIMPMEGVGMVYDEGLVTSEETQTRLEHEFGPLEHVASPPGD
                     PARVWRIPGSAGTIGFISPISEPFCSTCNRVRLSADGKLRLCLLRADEVDLRDIVRNG
                     GTTEELTSRIRSAIWRKPWGHGIAEGDFNIGRGMSQIGG"
BASE COUNT          421 a          757 c          869 g          477 t
ORIGIN      
        1 ccggtgctgg cggcggcgat tgaccgacac gtcgtgattg ctatcagaga agatcccatc
       61 caggtcggcg tcagggccga cttcgcagat atccccgggg aattcgcaca cgtcttgcct
      121 gatctcgcgc acgactggcg aaacccggga caatcgaccg cgtcaggcga ttacctgcgt
      181 ggggcaatcg ctgcgctcca gactcgcggc caagccgtac gaaccggtct tgagctggtt
      241 gtggcgggtg atcttccata cggtgtcgga atcagcagct cagctgcgct ttgtgtcgct
      301 ctggtgttgg cgctcgcacg tgaaacgccg gcgccaagcg aggtcgtctt gatcgcgcag
      361 gaagcagagc accgggcagg atcgccatgc ggaaccatgg accaatctgc cagcgtctac
      421 ggaggcctca tccggtacga gggcgcggac aatagcgtgc ggacgatcgc ggcggacctc
      481 gccggtcacc agttcgtcgt cgtcaactcg ggcgtggttc ggacgctggc cggctcggcg
      541 tatcctgagc gggtccggga gacgcaagca gcgttggcga tcctgcaggg ccggtggcgc
      601 gaggatttgc ccgcccttgg ggcaatcgca tctgcggacc tggtggcggc gctggccgtg
      661 ctggaatcgg cgggcgccgc gaagctggta aaccgcgtgc gccacgtcgt gacggaaggg
      721 cagcgggtgc gcgatgccga gcgggccatc gccggatcag attgggccga agttggacgg
      781 ttgatgaccg ccagcggcca ctcatcggca tcggactacg acatgagtca tccgcaggtc
      841 gagcgcgtcg tctcgctgtg cctggacgtg ccgggcgtcc tcggggcgcg gatgatgggc
      901 ggaggtcagg gtggatcggc gctggttctc gctcgcagcg agtccgtgga tgcgctgcgt
      961 gaccgactgg accgcgacta cttcaatgaa cttcggaacc cgcccggcgt gcgcatgatc
     1021 ccgtgcacat ttgcgcccgg cgccggaccg atggagccag cctagcggga atagcgggat
     1081 ggcaaggcgt gcttctgctt gcgcttgacg tcccggatgc ccgatcgccg gtttcggtag
     1141 gcggtcgtca ggtggctacc ggtcataggg tcgaggacga ggaccgtgcc ctcgagccgg
     1201 gtcacgctgc tctcgcgctg gtctgcggcg gggatgtccc ggcgacccag gcagatgaag
     1261 acggctttgc cgttgcgaat gcgtctccca tggttgagca catactcgat gtcgcgcgtt
     1321 gacaggttgc gctgcgcgag tcgctgggat gcgtggatgg agacggattc aagttgacga
     1381 tcggccatcg acaaagtcct tacacaatat ctcctgacgt caattgtttc ggatgtacgt
     1441 acagtgtgtc cgtatgtcgc cgatttgtca atagggtcga tgctgcccca ttggtaatcc
     1501 aacgaatggg ccagccggga ggttggcata tgctcaagat atatgcagat ttacccagga
     1561 atcgagtgtg cgcaggctac ccgccgattt gcgacatgcc ccgaccgatg ttgaaatcgc
     1621 cctcggcgat gccgtggccc cacggcttcc gccagatcgc ggatcggatc cggctggtca
     1681 attcctcggt tgtgccgccg ttccggacga tgtcgcgcag gtcgacctcg tcggcccgga
     1741 gcaggcagag tcgcagcttg ccgtctgcgc tcaggcggac ccggttgcag gtgctgcaga
     1801 agggctcgga gatcggcgag atgaagccga tcgtgccggc tgatcccggg atgcgccaga
     1861 cccgggctgg atcgccgggc ggagaggcaa cgtgctccag cggtccgaac tcatgctcca
     1921 gccgcgtctg ggtttcctcg gacgtgacca gaccctcgtc gtagaccatg ccgacgcctt
     1981 ccatcggcat gatctcgagg aagcgcatct gccaggggcg gtcaatggtc aggcgagcca
     2041 gttcgtctac ctcgtgctcg ttctggccac gcacgacaac ggtgttgatc ttgatcggcg
     2101 tgatcccggc ccgctctgcg gcgaggaggc cttcccagac catgtcgaaa cggccgccgc
     2161 gggtcatcgc cttgaaccgg tcggggttca gcgtgtcgag gctgacgttg attcggtcga
     2221 gtcccgcgtc ggccagcccg tccgccatgc gaccgagcag cagtgcgttc gtggtcatgg
     2281 tgacctcctc gacgccgggg aactgcttga tcgcccggac gatgtcgacg aggtgtgggc
     2341 ggatggtcgg ctcgccgcca gtcagccgga atttggtgaa gccgagcccc gcgaacgcgc
     2401 cgacgacctt gatgagctcg tcatcggtca gaatttcctc gcgcggctga aatttcatgc
     2461 ccatttccgg catgcagtag acgcagcgga agttacagcg atcggtcagg gagatgcgca
     2521 ggta
//
LOCUS       DASSGX010000013         2648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_655186, whole genome shotgun sequence.
ACCESSION   DASSGX010000013 DASSGX010000000
VERSION     DASSGX010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655186"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1892)
                     /gene="ppsA"
                     /locus_tag="VFI12_00200"
     CDS             complement(<1..1892)
                     /gene="ppsA"
                     /locus_tag="VFI12_00200"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: protein motif:HMM:NF005057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HET7054844.1"
                     /translation="MIDWFEGLSRGDVARVGGKNASLGEMVRTLGAEGIKVPPGFATT
                     ADAYWSFIDGNNYRDEIGALILDWTEGRMSLAEAGSAVRQLVLQGNWPEQLAASITES
                     YRELCRRTGQKGVAVAVRSSATAEDLPDASFAGQQETFLNIRGEADLLIACRRCYASL
                     FTDRAISYRQTKGFDHRKVALSIGVQQMVRSDLGGSGVMFSIDTETGFDKVVLINAAW
                     GLGENVVQGAVDPDEYVVFKPFLGNPALTPIIEKARGEKAIKMIYAAGERPTRNVPTS
                     KAERAAFVLSDAEILKLARWATTIEKHYGCPMDMEWARDGVTDEIFIVQARPETVQSR
                     RTEGEMRSFTISQKGRTLASGIAIGDAVVTGQVCLIEDVKDIDKFVDGAVLVTSTTDP
                     DWVPVMKRAAAIVTDHGGRTSHAAIVSRELGLPAIVGTGNATHVLHSGQDVTVSCAEG
                     DEGFVYEGTATFETSTLDVTQLPKTRTHLMLNLANPGAAFRWWRLPADGVGLARMEFV
                     IANAIRVHPMALLKFEELKDEAAKAEIAKLTEGYADKAEYFVDRLARGLARIAALAYP
                     QPVIVRMSDFKTNEYAGLVGGRQFEPAEENPMLGFRGASRYYSPKYREGFALECRAIE
                     RLRQSFG"
     gene            2045..>2648
                     /locus_tag="VFI12_00205"
     CDS             2045..>2648
                     /locus_tag="VFI12_00205"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HET7054845.1"
                     /translation="MSENSRHALVGAIGGTYISLAIMDIDEYTVANFALLNSADFASP
                     MEAIARYLKSLPRVPDKVGLSIAGLVNGESARMTHLPWSFDWNDIRAVTRASHICFVN
                     EFEALALATPTLSSYDVLPVARGAIKRTANRAVVSAGTGLGAAALVWTGSRWQALSGA
                     SRFASFPAPQGHEFDIRTIVAHEGFVEAGQILTGHGMVTLY"
BASE COUNT          481 a          870 c          802 g          495 t
ORIGIN      
        1 ccgaatgact ggcgcaggcg ctctatggct cggcactcga gcgcaaagcc ctcgcgatac
       61 ttgggcgagt agtagcggga cgccccgcga aaccccagca tcggattctc ctccgccggt
      121 tcgaactggc gtccgcccac gagaccggcg tactcgttgg tcttgaagtc gctcatccgg
      181 acgatgaccg gctgcgggta ggcgagcgcg gcaatccggg ccaagccgcg cgccagccgg
      241 tcgacgaagt attcggcctt gtcggcgtag ccttcggtga gctttgcgat ctcggccttg
      301 gcggcctcat ccttgagctc ctcgaatttg agcaatgcca tcggatggac gcggatggca
      361 ttcgcaatga cgaattccat acgagcgagg ccgaccccgt cggccggaag ccgccaccag
      421 cggaaggccg caccaggatt ggcgaggttc aacatcaggt gagtgcgtgt cttgggtaac
      481 tgggtcacat cgagagtcga cgtctcgaac gtggctgtgc cctcgtacac gaacccttca
      541 tcgccttcgg cacaggagac ggtcacgtcc tggccgctgt gcagcacatg ggtagcgttg
      601 ccggtcccaa cgatcgccgg cagcccgagt tcccggctca cgatcgcggc gtgcgaggta
      661 cggccgccat gatcagtgac gatcgcggca gcccgcttca ttaccggcac ccaatcgggg
      721 tccgtcgtgg acgtcacgag aaccgcacca tcgacgaact tgtcgatatc cttgacgtct
      781 tcgatcagac acacctggcc ggtgacgacg gcatcgccga tggcgatccc cgaggcgagt
      841 gtccggccct tctgcgaaat cgtgaagctg cgcatctcac cctcggtgcg gcgcgactgc
      901 accgtctcgg ggcgagcctg cacaatgaaa atctcatcgg tcacgccatc gcgcgcccat
      961 tccatgtcca ttgggcagcc atagtgcttt tcgattgtcg tcgcccatcg ggcgagcttc
     1021 agaatctccg cgtcggaaag gacgaaggcc gcacgctccg ccttggacgt cggaacgttg
     1081 cgcgtcggcc tctcgcctgc cgcgtagatc atcttgatgg ccttttcgcc gcgtgccttc
     1141 tcgatgatcg gtgttagggc cgggttgccg agaaacggct tgaagaccac gtattcgtcc
     1201 gggtcgacgg cgccctgcac gacgttctca cccaggcccc aggcggcgtt gatcagcacc
     1261 actttgtcga agccggtctc ggtatcgatc gagaacatca caccggaccc accgaggtcg
     1321 gagcgcacca tctgctgcac gccgatcgac agcgccacct tgcggtggtc gaaacccttg
     1381 gtctgccggt aactgatggc ccggtcggtg aagagcgacg cgtagcaacg gcggcaggcg
     1441 atcagcagat cggcctcacc ccggatgttg agaaaggtct cctgctgacc agcgaagctt
     1501 gcgtccggaa ggtcttcggc agtcgcgctc gaacgcaccg cgacggcaac gcccttctgc
     1561 cccgtccgac ggcagagctc gcgatacgat tcggtgatcg acgcggcgag ctgttccggc
     1621 caattcccct gcagcaccag ctggcgtact gcgctgccgg cttccgcaag gctcatccgg
     1681 ccctcggtcc aatcgaggat caaagcaccg atctcgtcgc gatagttatt gccgtcgatg
     1741 aacgaccaat aggcgtcggc cgtggtcgca aagccggggg gcaccttgat gccttcagcg
     1801 ccaagggtcc gcaccatctc tccgagcgaa gcgttcttgc cgccgacgcg tgccacatcg
     1861 ccacggctca ggccttcaaa ccaatcgatc atggggagtt tcttgagcat tccaaacctc
     1921 tccgccgcct tggcgacatt gcgtgccgga acactggcgt gccgccgact gcgggacttt
     1981 gatcggcatc aaatggagat cgcgtgggtc cggcgatagt accgctgcaa cacggaggtg
     2041 caggatgagc gaaaattcac gtcatgcctt ggtcggcgcg atcgggggga cctatatcag
     2101 cctcgcgatc atggatatcg acgagtacac cgtcgcgaat ttcgctctcc tcaacagcgc
     2161 tgacttcgcg agtccaatgg aagcgattgc acggtatctg aagtcgcttc cgcgcgtgcc
     2221 cgacaaggtc ggtctttcga tcgccgggct ggtcaacggc gaaagcgcaa gaatgaccca
     2281 tctgccatgg tcattcgact ggaacgacat ccgggccgta acgcgcgcca gccacatctg
     2341 cttcgtcaac gaattcgagg ccctggcact cgccaccccg accctgagca gctacgatgt
     2401 tctgccggtc gcgcgcggcg cgatcaagcg aaccgccaat cgtgccgttg tcagtgccgg
     2461 cactggtctc ggcgccgctg ccctggtctg gaccggatcg cgctggcaag cgctctctgg
     2521 cgccagccgc tttgcctcct tcccggcccc ccagggccat gaattcgaca tccggaccat
     2581 cgtggctcat gaaggcttcg tcgaagctgg gcagatcctg actggccacg gcatggtgac
     2641 gctataca
//
LOCUS       DASSGX010000014         2153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_328596, whole genome shotgun sequence.
ACCESSION   DASSGX010000014 DASSGX010000000
VERSION     DASSGX010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328596"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2153)
                     /locus_tag="VFI12_00210"
     CDS             complement(<1..>2153)
                     /locus_tag="VFI12_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7054846.1"
                     /translation="SMSETAHSIATPDTAALPGSLPGVSAPARERARELAERAWFPDA
                     LGLIGVLLVTAVLAWHRLYLENGLAYLDVATFYMPWYAHLGEAVRSLDIPGWNPYVFS
                     GTPFAGDPQSGWWYFPAMAIFGLFDPVHAYQIFIIFHLAFAGVTTYILCRMFGLQAIP
                     SLLAGAAYQSGPFVSHVSCCLIHVQLATWIPMALIGVETTVRAKHANRRAVGWVITGF
                     AMSQMIAGWPGQGMYNGCLLTGGYLAFRILLSHENGVLGWRDRIVRLIGDSAGVLSFA
                     LLLAAAGILPRIDIVSRTNVAWGEYDGFETDSYASGWQIYTVLDRLFTDNNGYRSLLF
                     YLGAPVIILMFIGVILSWRRQWVKFMTAVTILTSIMTLKPTFIHQIVFLLPRYEALHN
                     HVPSRILAVQWIAPVILAATALDTLMRSPSRDRILKAVGIAFGIWTGVALLLQVGNRP
                     VPIAVLITAVMTAGVVCLFAIVTTPESDRYGRFVPRAQAGLAILLLLLVIGDPAGRRL
                     VATLETGQTMSEVIRIPTGPVSRAAVRENDASADPGGAGEFLQEKRDAGEYFRYFGYD
                     NVLQQAGEGYPSTYRENFGNPTALAILVNARAMRLHLNDVQGYNPVQLRNYVTYLNDM
                     NDAVQNYHDAQILPGGLNSPLLNILNARYIIIPNEAVGMRPRDDIMLLVASYPEVFRN
                     DTVRVLENPNAMPRAWIAHDVRTAPPELIPDMIKA"
BASE COUNT          406 a          683 c          723 g          341 t
ORIGIN      
        1 cggccttgat catgtccggg atcagctcgg gcggtgcggt gcggacatcg tgcgcgatcc
       61 aggctcgggg catcgcgttc ggattttcaa gcacgcgcac cgtgtcattc cggaagacct
      121 ccggatacga cgccaccagc agcatgatgt cgtcccgggg ccgcatcccg accgcttcgt
      181 tcgggatgat gatgtatcgc gcgttaagga tgttgagcag cggcgaattc aggccgccgg
      241 gcaggatctg cgcgtcgtgg tagttctgga ccgcgtcgtt catgtcattc aggtaggtga
      301 cgtagttgcg gagctggacc gggttgtacc cctgcacgtc gttcaggtgc aggcgcatcg
      361 cccgggcatt gacgagaatc gcgagtgcgg tcgggttccc gaagttctcg cggtaggtcg
      421 agggatatcc ttcgccggcc tgctggagga cgttgtcgta gccgaagtag cggaagtatt
      481 cgccggcatc ccgcttctcc tgcaggaact ctcccgcccc gcccggatcg gccgacgcgt
      541 cattttctcg caccgcagcc cggctgaccg gcccggtcgg aatccggatc acctcggaca
      601 tcgtctgccc ggtctcgagg gtcgcgacga gccgccgccc ggccggatcg ccgattacga
      661 gcagcagcag caagatcgcg agtccggcct gcgcgcgagg cacgaaccga ccatagcgat
      721 cactctctgg cgtcgtgacg atcgcgaaga ggcagacgac cccggccgtc atgactgccg
      781 tgatcagcac ggcgatcggg acgggccggt tcccgacctg gagcagcagc gccaccccgg
      841 tccagatgcc gaacgcgatc ccgaccgcct tcaggattcg gtcccgcgag ggagagcgca
      901 tcagcgtgtc gagcgcggtc gccgcgagga tcacgggagc aatccactgg accgcgagga
      961 tccggctcgg cacatggttg tgcagcgcct cgtaccgcgg cagcaggaag acgatctggt
     1021 ggatgaaggt cggcttgagc gtcatgatcg aggtcagtat cgtgaccgcc gtcatgaact
     1081 tgacccactg ccgccgccag ctcaggatca cgccgatgaa catcaggatg atgactggcg
     1141 cgccgaggta gaagagcagc gatcggtaac cgttgttgtc ggtgaagagc cggtcgagca
     1201 ccgtgtagat ctgccagccc gaggcgtagc tgtccgtctc gaaaccgtcg tactcgcccc
     1261 aggcgacatt cgtgcggctg acgatgtcga tccggggcag gattccggcc gccgccagga
     1321 gcagcgcgaa cgagagcacg cccgcgctgt cgccgatcag ccggacgatg cggtcccgcc
     1381 agccgagcac gccattctcg tgcgagagca ggatccggaa ggcgaggtag ccgccggtca
     1441 ggaggcagcc gttgtacatc ccctgccccg gccacccggc gatcatctgg ctcatcgcga
     1501 aaccggtgat gacccagccg acggcccgac gattggcatg cttggcccgg accgtcgttt
     1561 ccacgccgat tagcgccatc gggatccagg tcgcgagttg gacatggatc agacagcaac
     1621 tcacatgcga cacgaacggg cccgattggt acgccgcacc cgccagcaga ctcgggatcg
     1681 cctgcaagcc gaacatccgg cagaggatgt aggttgtcac gccggcgaac gcaaggtgga
     1741 agatgatgaa gatctgatag gcatggaccg ggtcgaacaa cccgaagatg gccatcgccg
     1801 ggaaatacca ccagccggat tgcggatcac cggcaaatgg cgtgcccgag aaaacgtacg
     1861 ggttccagcc cgggatatcg agcgagcgga ccgcctcgcc aaggtgcgcg taccagggca
     1921 tgtagaaggt ggcgacgtcg aggtaggcca agccgttctc gagatacagc cggtgccagg
     1981 cgagcaccgc cgtgaccagc agcacgccga tcaagccgag ggcatcgggg aaccaggcgc
     2041 gctccgccag ctcccgcgcg cgctcccggg ccggcgccga aacgcccggc aatgatccgg
     2101 gcagcgcggc cgtgtctgga gttgcgatcg aatgtgcggt ttcggacatc gaa
//
LOCUS       DASSGX010000015         2825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_763918, whole genome shotgun sequence.
ACCESSION   DASSGX010000015 DASSGX010000000
VERSION     DASSGX010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_763918"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VFI12_00215"
     CDS             <1..541
                     /locus_tag="VFI12_00215"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7054847.1"
                     /translation="QVPPDFKRIALSRPLGIPTPIIIAVGLGLVFAWLLRYSRVGRML
                     YAIGSNPDAARPAGIQTNRVVFGAYVACGVLCGVGGVLWGARFGSIDARAASGMELQV
                     VAAVVLGGVNIFGGSGTIVGAMLGAVLLGTIQNALTILRLNQFWIQAISGGAILLAVT
                     VDLLITRRLRRSLTLRRAT"
     gene            538..1548
                     /locus_tag="VFI12_00220"
     CDS             538..1548
                     /locus_tag="VFI12_00220"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7054848.1"
                     /translation="MRPGRHWLTWEILLLAMLLALIATGWATSGVFGQGSTVTLIITN
                     SMEKALMALPMTLIIISGEIDLSVASTLGLASAVIGKTWAAEWPLWLCIAAALVVGAL
                     CGLLNGLLVTKLGLPSLVVTLGTLALYRGLAYVILGSQAVSDFPGGFASFGFKSIPGT
                     RLPWTVLVFIPLLIVLLILLHRSWIGRQLYAAGSNAEAARFAAVRVNQLKLWLFVLSG
                     VVSAIAGVIFTARVSSSRADNATGFELDVVAAVLLGGVSIFGGSGTLIGVVLSLAVIG
                     TLRQVLALNSSNAELQSIAIGSLLILSVLGPNLARSAAGLRTGRQFAGMRPRGAAPAD
                     QV"
     gene            1620..2732
                     /gene="rhaS"
                     /locus_tag="VFI12_00225"
     CDS             1620..2732
                     /gene="rhaS"
                     /locus_tag="VFI12_00225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0033296 - rhamnose binding [Evidence IEA];
                     GO_process: GO:0015762 - rhamnose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnose ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7054849.1"
                     /translation="MKNQHLFTRLAIAMLLLLSAMTGLGASSHALAQAADCATGATGG
                     AASPEASPSPGGATGGASPAAMAGINMAFVPKDIVNNYFAASFKGAQEAATETGGTVQ
                     QVGPDTPDAAGQVQFIQTLTQQGVQAIATSANDPNALAPALKDARAKGIKVVSYDSDV
                     AQDARDIFVNTANSEDIGRIQIQIISRLIGCSGEIAILSAASTATNQNTWIDFMKDEL
                     TKPGYENVKLVETVYGDDKPQLSYDKTVELLTAYPDLKGIVSPTTVGLAAAASALEAE
                     GRTDVQLTGLGLPNEMRQYIKSGTVKEMALWNPIDMYYLTYYAAAALVSGEITGAEGD
                     TFTAGHLGQYTVGANGVVLLGKPFIFTADNIDQFDF"
BASE COUNT          456 a          922 c          964 g          483 t
ORIGIN      
        1 gcaagtcccg cccgatttca agcggatcgc cctctcgcgg ccgttgggca ttccgacgcc
       61 gatcatcatc gcggtcgggc tcgggctggt ctttgcctgg ctgctgcgct actcgcgggt
      121 cgggcggatg ctctacgcga tcggcagcaa cccggatgcg gcgcggccgg ccggcatcca
      181 gaccaaccgg gttgtcttcg gcgcgtacgt tgcctgcggg gtcctctgcg gagtcggggg
      241 cgtgctctgg ggggcgcggt tcggctcgat cgatgcccgg gcggcgagcg gcatggagct
      301 gcaggtcgtg gcggcggtcg tcctcggcgg ggtgaacatc ttcggcgggt cggggacgat
      361 cgtcggggcg atgctcggcg cggtgctgct cgggacgatc cagaacgcgc tgacgatcct
      421 gcggctgaac cagttctgga ttcaggcaat ctccggcggg gcgatcctgc tcgcggtcac
      481 ggtcgatttg ctgatcaccc ggcgactgcg gcgctccctg accctgaggc gggcgacatg
      541 agaccgggac gacactggct gacctgggaa atcctgctgc tcgccatgct cctggcgctg
      601 atcgccaccg gctgggcgac gtccggagtc ttcgggcaag gctcgacggt gacgctgatt
      661 atcaccaaca gcatggaaaa ggcgctgatg gccctgccga tgacactgat catcatctcg
      721 ggcgagatcg acctttccgt ggcgtcgacg ctgggcctgg cgagcgcggt gatcggcaag
      781 acctgggcgg cggagtggcc actttggctc tgcatcgccg cggcgctggt cgtcggggcg
      841 ttgtgcgggc tgctgaacgg cctgctggtg acgaagctcg ggctgccgtc gctggtggtc
      901 acgctcggca cgctggcgct ctatcgcggt ctggcgtacg tgattttggg cagccaggcg
      961 gtcagcgatt tcccgggcgg attcgcctcg ttcggcttca agtcgattcc cggcacccgc
     1021 ttgccgtgga cggtgctcgt cttcatcccg ttgctgattg tgctgctgat cctgctccac
     1081 cggagctgga tcgggcggca gctctatgcg gccggcagca atgccgaagc ggcgcgattc
     1141 gcggcggtgc gggtcaacca gctcaaactc tggctgttcg tgctgagcgg cgtggtctcg
     1201 gcgatagccg gcgtgatctt cacggcgcgg gtctcgagca gccgggccga caacgcgacc
     1261 ggcttcgagc tggacgtggt agcggcggta ttgctcggag gggtgagcat tttcggcgga
     1321 agcggtacgc tgatcggcgt cgtcttgtct ttggccgtga tcggcacgct ccgccaggtg
     1381 cttgccctca attcctcgaa tgcggagctg cagagcatcg ccatcggaag cctgctgatt
     1441 ctctccgtgc tcggtccgaa cctcgcgaga agcgcggccg ggctccgcac gggtcgtcaa
     1501 ttcgccggaa tgcggccgcg cggggcagcc cccgccgacc aggtctagaa cggagggaag
     1561 tgcgcaacag gcaggttgag aaacgatcga tgacgatcat gacccgcgag gagggtttga
     1621 tgaagaacca gcacctgttt acccgtctgg caatcgcaat gctcctcttg ctctccgcca
     1681 tgaccggtct cggcgcgagc agccacgcgc tggcccaggc ggccgactgc gcgaccggtg
     1741 caaccggcgg cgcggccagc ccggaggcgt ccccatcgcc gggcggcgca accggcggcg
     1801 cgtcgccggc agccatggcc ggcatcaaca tggccttcgt ccccaaggac atcgtcaaca
     1861 actacttcgc cgcctccttc aagggcgcgc aggaagccgc gaccgagacc ggcggcacgg
     1921 ttcagcaagt cggcccggac accccggacg cagccggcca ggtccagttc atccagacgc
     1981 tgacgcagca gggcgtgcag gcgatcgcca cgtcggcgaa cgacccgaat gcgctcgcgc
     2041 cggccctcaa ggatgcccgc gccaagggaa tcaaggtcgt cagctacgac tcggacgttg
     2101 cccaggacgc gcgcgacatc ttcgtcaaca cggccaatag cgaggacatt ggccgcatcc
     2161 agatccagat catctcccgg ctgatcggtt gctccggcga gatcgcgatt ctctcggccg
     2221 ccagcaccgc gaccaaccag aacacctgga tcgacttcat gaaggatgag ctgaccaagc
     2281 cgggctacga gaacgtcaag ctcgtcgaaa ccgtctacgg cgacgacaag ccgcaactca
     2341 gctacgacaa gacggtcgag ctcctgaccg cctacccgga cctgaagggc atcgtctcgc
     2401 cgacgacggt cggtctggcc gccgcggcat ccgcgctcga ggcggagggc cggacggatg
     2461 tccagctcac cgggcttggc ctgccgaacg agatgcggca gtacatcaag agcggcaccg
     2521 tcaaggagat ggcgctctgg aacccaatcg acatgtacta cctgacctac tacgccgcgg
     2581 cggcgctggt cagcggcgag atcaccggcg ctgagggcga caccttcacg gctggtcacc
     2641 ttgggcagta cacggtcggc gcgaacggcg tcgtcctgct cggcaagccg ttcatcttca
     2701 ccgcggacaa catcgaccag ttcgacttct agctgcgagc acggacgtag cgcggtcccc
     2761 ttgtggaccg ccaatgaaca ccgtggccgg cgcaaatttg cgccggccac ggtgttcatt
     2821 ggcgg
//
LOCUS       DASSGX010000016         4264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_328837, whole genome shotgun sequence.
ACCESSION   DASSGX010000016 DASSGX010000000
VERSION     DASSGX010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328837"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1004
                     /locus_tag="VFI12_00230"
     CDS             <1..1004
                     /locus_tag="VFI12_00230"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HET7054850.1"
                     /translation="YAQGGILRERFIPRLMAAEAKSLIDAERDNFDPFAVWRTFMANE
                     KPGGHGDRAHAAGALDMAVWDAVAKIAEQPLYRLLADRYRRGVVDERVFVYAAGGYYQ
                     PGKDLRALQDEMHGYLDMGYSVVKMKIGGDSLAEDLRRIEAVIAVIGSGSRLAVDANG
                     RFDLPTAIAYAEALAPYGLFWYEEAGDPLDYVLQAELAHHYDGPMATGENLFSMPDAR
                     NLIRYGGMRPDRDWLQFDCALSYGLVEYLRTLEMLDAHGWSVRRVIPHGGHQMSLNMA
                     AGLGLGGNESYPGIFQPFGGFADGIAVEDGYVRLPEIPGIGFEAKAELIGLMRTLAA"
     gene            complement(1007..2524)
                     /locus_tag="VFI12_00235"
     CDS             complement(1007..2524)
                     /locus_tag="VFI12_00235"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET7054851.1"
                     /translation="MRIDKNAPVPIARQIERAIGDLIASGEFSPGQRLPTEMELCNRL
                     GISRTPIRQALGQLVAQGLLVRQAGRGTFVAINGAPAASNADSPEDVTVTVPEQRWCW
                     PLQQAALAWNAEHPDRQVRLRFKMVGQAELRGRLMQAVAQGRATDLALLDSVWVAEFA
                     ERGYLQPLASINPAVTSVLTADIVAPLLAEHTVHGELIALPADADFALLWYRKDWFAA
                     EGLAPPRTWDELLACARHFQRADVRDRYDLGLYPLTFAGGSVAGEATAYQLLPILWSA
                     GADVIANHHVVLNSRATRSAVTFIANLVQRHGIASRGVVTATWNDPPLAMAAGMAAMS
                     FGGSYENALIRAAAGWNEAEFLARIGFVPIPAGPGGSQATVLGGLSYGIFHQSRQPEL
                     ALGLLERAFAPDILRVFCAQTGQNPPTVSATRALAAETEPFLHATAQLLPYARSRWPI
                     AEYARVSAQLVRMFESAILAEASPEDAVARASAIIAGITGLPERTRHRPPAWPAA"
     gene            2947..>4264
                     /locus_tag="VFI12_00240"
     CDS             2947..>4264
                     /locus_tag="VFI12_00240"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7054852.1"
                     /translation="MGSDATLHTLAGQLRSGRIDRRRFMQGAAALGATATAISSALRA
                     MPTVAQGTTEVTFWTTYVANDLANVQAVVDGYNAQAQGNQVKLVQIPPAQVTDTTTLM
                     TAVRGGTGPDLYLFDRFIVAQRAGDGLLQDLSSLGADQLMGNYVPFAAAESQFNGKVY
                     CLPFDTDVRALYYNKGMLGADADKLDPANGPATWDTVAEVANKLNQQDGNGNYSQMGF
                     VPWVNQGWHYTYGFAFGGSFMDYSACSVTPDDPPIVDAFKWVQDYCVALDADKVNAFG
                     SPSMQPGADPAQHPFHIGKLGMQITGDWEIGQMELYAKDVDYGITWMPVPKTGDNSFT
                     WAGGWSVVIPQGAKNVDAAWQAMQWICGPDGGRIYTQKSSHVPVYAALYEEKDLFSER
                     HQFFLKLIPTAKNRPPLPVGLKYWDELSAAWQKDYLGQGQPADLLKA"
BASE COUNT          739 a         1300 c         1536 g          689 t
ORIGIN      
        1 gttatgccca gggtggaatc ctgcgtgagc gtttcatccc gcggctgatg gcggccgaag
       61 ccaaatcgct gattgacgcg gagcgggaca atttcgatcc gttcgccgtc tggcgcacct
      121 tcatggccaa cgagaaaccg ggcggccatg gcgaccgggc gcacgcggcc ggcgcgctcg
      181 acatggcggt ctgggatgcg gtggccaaga tcgcggagca gccgctgtat cgcttgctgg
      241 ccgatcgcta tcgccgtggc gtggttgacg agcgggtgtt cgtctatgcg gccggcggct
      301 attaccagcc gggcaaggat ctgcgcgcgc tgcaggatga gatgcacggc tacctcgaca
      361 tggggtactc ggttgtcaag atgaagatcg gcggcgattc gcttgccgag gatctgcgcc
      421 ggatcgaggc cgtgatcgcg gtgatcggat ccgggagcag gctggccgtc gatgccaatg
      481 gccgcttcga cttgccgacc gcgatcgcct acgccgaggc gctcgcgccc tatggcctgt
      541 tctggtatga ggaggccggc gatccgctcg attacgtgct ccaggcggaa ctcgcgcacc
      601 actatgacgg gccgatggcg accggcgaga atctcttctc gatgccggat gcgcgcaatc
      661 tgatccgata cggcgggatg cggccggatc gcgactggct gcagttcgat tgcgccctct
      721 cctacgggct ggtcgagtac ctgcgcacgc tcgagatgct cgatgcgcat ggctggagcg
      781 tccgccgggt gattccgcac ggcgggcatc agatgtcgct caacatggcg gccggactcg
      841 gattaggcgg caacgagtcg tacccgggca tcttccagcc gttcggcggg tttgccgacg
      901 ggattgcggt cgaggacggc tatgtgcggc tgccggagat tcccggcatc ggtttcgaag
      961 caaaggccga gttgatcggg ctgatgcgca ccctggcggc gtagggtcac gcggcgggcc
     1021 aagcgggggg gcggtggcgg gtgcgctcgg ggaggccggt gatgccggcg atgatggcgg
     1081 aggcccgggc gacggcgtcc tccgggcttg cctcggccag gatcgcgctc tcgaacatgc
     1141 ggacgagctg ggccgagacc cgggcgtact ccgcgattgg ccagcgggag cgcgcgtagg
     1201 gcaggagctg ggcggtggcg tggaggaagg gctcggtttc ggcggccaag gcgcgggtcg
     1261 cggagacggt cggcgggttc tgcccggtct gggcgcagaa gacgcgcagg atgtcaggtg
     1321 cgaaggcccg ctcgagcagg ccgagcgcca gttcaggttg ccgggactgg tggaagatgc
     1381 cgtagctcag gccgccgagc acggttgcct ggctgccgcc cgggccggcc gggatcggca
     1441 cgaagccgat ccgcgcgaga aattcggcct cgttccagcc cgccgccgcc cggattaggg
     1501 cgttctcata gctgccgccg aacgacatgg cggccatgcc ggcggccatg gccaacggcg
     1561 gatcgttcca ggtggcggtc acgacgccgc gcgacgcgat gccatgcctc tggacgaggt
     1621 tggcgatgaa ggtgacggcg ctgcgggtcg cgcgggagtt gagcacgacg tggtggttgg
     1681 cgatcacgtc cgctccggcg gaccagagga tcggcaagag ctgatacgca gtcgcctcgc
     1741 cggccacgct gcccccggcg aaggtcaggg gatacaggcc gaggtcgtag cggtcgcgca
     1801 cgtctgcgcg ctggaaatgg cgagcgcagg cgagcaactc gtcccaggtc cggggtggcg
     1861 ccagcccttc ggcggcgaac cagtctttgc gataccagag cagggcgaaa tcggcatcgg
     1921 ccggcagcgc aatgagctcg ccgtggacgg tgtgctcggc caacagtgga gcgacgatgt
     1981 cggcggtgag gacgctggtg accgccgggt tgatcgacgc gagcggctgg agatagccgc
     2041 gctcggcgaa ctccgccacc cagaccgaat cgagcagggc gaggtcggtc gcgcggccct
     2101 gggctacggc ctgcatgagt cgcccgcgca gctcagcttg tccgaccatc ttgaaccgga
     2161 ggcggacctg gcgatcggga tgctcggcat tccaggcgag tgcggcctgc tggagcggcc
     2221 agcaccagcg ctgctcgggg acggtgacgg tgacgtcctc gggggaatcg gcgttggagg
     2281 cggccggagc gccattgatc gcgacaaacg tgccgcgccc ggcttggcga acgaggagtc
     2341 cctgggcgac gagctggccg agggcttggc ggatcggagt ccggctgatg cccaggcggt
     2401 tgcagagctc catttcggtc ggcaggcgtt ggcccgggga aaactcgcca ctggcgatca
     2461 gatcgccgat agcccgctcg atctggcggg caatgggaac gggcgcattt ttgtcgattc
     2521 gcatggtggg caccgtatgt cctgcgccac gggacacgat tgccaggacg gagcgcactc
     2581 gagcccggtg aatggatagt acatttggaa cacgattatg ggaagcaccc tcgctctgaa
     2641 accagggaca ggccgtcaga gcagaccgat atgcctgcga cgggtgcccg gccacgaggc
     2701 gccgtaggcg gaatgtagca ttggcatcac gtggcgggca gcaggcattg ccaaattggc
     2761 aataacaaat agtgccaaag tattgacttt gccgttgtcc gaacatattg tcaggatcac
     2821 cgattgtccg tgccgctatc gaataccggg caccgagcag tcggcggaac ctggtggcag
     2881 gttcttcgga ttgtcgagtc accggaatct cggcgaggcc gtctccggtg cgcacggagg
     2941 acaggaatgg gcagcgacgc tacgcttcat accctcgccg gacaattgcg aagtggccga
     3001 atcgaccgac gtcgattcat gcagggagcc gccgcgctcg gcgcaacggc gaccgcgatc
     3061 agcagcgcgc tccgggcgat gccgaccgtc gcgcagggca caaccgaagt caccttctgg
     3121 acaacgtacg tcgccaacga ccttgccaat gtgcaggcgg ttgtcgacgg gtacaacgcg
     3181 caggcgcagg gcaaccaggt caagctggtc cagatcccgc cggcgcaggt gaccgacacg
     3241 acgacgctga tgacggcggt ccgcggcggg accggcccgg atctctacct cttcgaccgc
     3301 ttcatcgtgg cccaacgggc cggcgatggg ttgttgcagg atttgagctc cctcggcgcc
     3361 gatcagttga tgggcaatta cgtcccgttc gccgcggccg agtcgcagtt caatggcaag
     3421 gtctactgcc tgccgttcga caccgacgtg cgcgccctct actacaacaa gggcatgctc
     3481 ggggcggatg ccgacaaact cgacccggcc aatggcccag cgacgtggga tacggtcgcc
     3541 gaggtcgcga acaaactgaa ccagcaggac ggcaacggca actacagcca gatgggcttc
     3601 gtgccgtggg tcaaccaagg ctggcattac acctacggct tcgccttcgg cggcagcttc
     3661 atggattaca gcgcctgctc cgtgacgccc gatgatccgc cgatcgtcga cgccttcaag
     3721 tgggtgcagg actactgcgt ggcgcttgac gccgacaaag tcaacgcctt cggctcgccg
     3781 tcgatgcaac caggggccga cccggcgcag cacccgttcc acatcggcaa gctcgggatg
     3841 cagattacgg gcgactggga aatcggccag atggagctgt atgccaagga cgtcgactac
     3901 ggcatcacct ggatgccggt gccgaagacg ggcgacaact ccttcacgtg ggccggcggc
     3961 tggtcggtcg tcatcccgca gggcgcgaag aatgtcgacg cggcgtggca ggcgatgcag
     4021 tggatctgcg ggccggacgg cggccgcatc tatacgcaga agagcagcca cgtgccggtt
     4081 tacgcggccc tctacgagga gaaggatctc ttctccgagc ggcaccagtt cttcctcaag
     4141 ttgatcccga cggccaagaa ccggccgccg ctgccggtcg gcctgaagta ctgggacgag
     4201 ctgagtgcgg cctggcagaa ggactacctg ggtcagggcc agccggccga tctgctcaag
     4261 gccg
//
LOCUS       DASSGX010000017         3052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1526125, whole genome shotgun sequence.
ACCESSION   DASSGX010000017 DASSGX010000000
VERSION     DASSGX010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1526125"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VFI12_00245"
     CDS             complement(<1..548)
                     /locus_tag="VFI12_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7054853.1"
                     /translation="MSLRDRTFQTRRMTRLTGGVPEPHIEAAPPLTPAPPAPVPDAAP
                     QPVERPQADRPRVMLSREALELKHALHDRLVREIDPSRLTQNLSPEDARRAVEDAVLE
                     LLAKESANISRTDEMILMREIADEIVGFGPIEPLLQDETVTEVMVNSPDRVFFEKEGV
                     LFLSDRSFRDDDHIMRLIDKIVS"
     gene            complement(555..2036)
                     /locus_tag="VFI12_00250"
     CDS             complement(555..2036)
                     /locus_tag="VFI12_00250"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7054854.1"
                     /translation="MTSPATRVLIVDDNEKTRDNLIDLLRFDDIDVVGESTFGAAAYT
                     WAEQLDVDLVVVSIEEPVARSLRTVEALSTGARAWPVVGVSTQGDRDTMRKAMVSGVR
                     DFLTMPVSAEEMRTTILNILNVDRARRSKVEHGDVSRPLGTVITVAGFKGGIGKSTVS
                     SNVSVALAQQTQQHVALLDLDLQFGDAAVMLDVVPTGTIEQVAKEADRMDPQMIQSLL
                     VTHASRLKLLAAPSTPEAADEITGDTVRQTIELLSATNDFIVVDTAPHLDELSATAMD
                     LSTIVLVVVIPEVPCIRRTKAALTLMQSWGYSRDKVKLVVNRSHRRGEVSVAEIEQVL
                     DYPVYAQVPEDRSVARAISVGTPVAMSAPKSGAGSAFNDIARVLAGVPKPQQRLGLLR
                     RPGKITSQPLRQGRAAEARPQAQPALGIGRSSAGAAQAVQGESDFAGFSWVESWPGAA
                     APPSGTGTSVERNPWAITTNVGDAPDMARAANSHAVVASSSQE"
     gene            complement(2071..>3052)
                     /locus_tag="VFI12_00255"
     CDS             complement(2071..>3052)
                     /locus_tag="VFI12_00255"
                     /inference="COORDINATES: protein motif:HMM:NF018095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HET7054855.1"
                     /translation="QDVEVLAVSQALTPEELGVEGQAVTPTPTVDGTDAVSVGEPVAR
                     PGAKSVTLAVTPEQAQRLLLAAQTVKNEKGSLRLAMRAPGDTTIANLPPAQLGNIPVG
                     DLLGDVDKPMVPSDVIITHAEFTERVISSGNVLEFKATVKNVSDHTIKSVKDARPGFE
                     YTQGVAYDALGFFPKEDTYRIGLSFAGAYPNPFPYRWGFGGNLEPGQSVEITGSVRLT
                     EATAATKYWLGIIQEPNTVTQDGVNVADVTVIQADAATVSADSADLRADPNGTAAVVG
                     EVAKGDTVEIVQARSAWFKVRAGKTEGWIEVSAVTVPPTGDQSASTDDGN"
BASE COUNT          471 a         1002 c         1013 g          566 t
ORIGIN      
        1 gagacgatct tgtcaatcag ccgcatgatg tggtcgtcgt cccggaacga gcgatcggat
       61 aggaagagga cgccctcctt ctcgaagaag acccgatcgg ggctattgac catcacctcg
      121 gttacggtct catcctggag cagtggctca atcgggccga acccgacgat ttcgtcggcg
      181 atctcgcgca tcaggatcat ttcgtccgtg cgcgatatat tggcgctttc cttggccagc
      241 aattcgagca cggcatcctc aaccgcgcgc cgggcatcct ccgggctaag gttctgggtc
      301 agccgggacg ggtcgatctc gcgaacgagc cggtcgtgca gcgcgtgctt gagttccagc
      361 gcctcgcggc tgagcatcac gcgtggccga tcagcctgcg ggcgctcgac cggctggggt
      421 gccgcatccg gcacaggggc aggtggggcc ggagtcagtg ggggcgcggc ctcaatgtgc
      481 ggttcaggaa ctccgccggt gagccgggtc atgcgtcggg tctggaaggt tcggtcgcgc
      541 agagacatgg ctgcttactc ctggctcgac gatgcaacaa cggcatgcga gttggcagct
      601 ctcgccatat caggcgcgtc accgacgttt gtggtgattg cccacgggtt gcgctcaacc
      661 gatgtcccgg ttccgctggg aggtgcggcc gctcccggcc agctctcaac ccacgagaac
      721 ccggcgaagt ccgactcgcc ctgcacggcc tgagcggctc cggctgatga ccgcccgatt
      781 cccagtgccg gctgcgcctg gggtcgcgct tcggccgcgc gaccctggcg gagcggctgg
      841 gatgtgatct tgcctggtcg ccgcaggagc cccagccgct gctgcggctt cgggacgccg
      901 gccaggaccc gcgcgatgtc gttgaacgcg ctccccgccc cactctttgg cgccgacatc
      961 gcgaccggcg tcccgaccga gatggcccgc gccaccgatc gatcttcggg aacttgcgcg
     1021 tagaccgggt aatcgagcac ctgctcgatc tccgcgaccg aaacttcgcc gcgccggtgc
     1081 gaccggttaa cgaccagttt gactttgtcg cgcgagtatc cccacgactg catcaacgtc
     1141 agcgccgcct tggtccggcg aatgcacggc acttcgggaa tgactaccac cagcacgatc
     1201 gtcgacaggt ccatggccgt cgccgagagc tcgtccaggt gcggcgccgt gtcgaccaca
     1261 atgaagtcgt tggtcgcgga cagcagctcg attgtttgcc ggaccgtgtc gccggtgatc
     1321 tcgtcggccg cttcgggcgt cgagggcgcc gccagcagct tcaatcgcga ggcatgcgtg
     1381 acgagcaagc tttggatcat ctggggatcc atccggtcgg cctctttggc cacctgctcg
     1441 atcgtaccgg ttggcacgac gtcgagcatg accgcggcat cgccgaactg gagatcgagg
     1501 tccagcaatg cgacgtgctg ctgtgtttgc tgcgccaacg ccacggagac attgctcgaa
     1561 acggtcgatt tgccgatccc gcccttgaat ccggcgaccg tgatgaccgt gccgagcgga
     1621 cgggatacgt ccccgtgctc gaccttcgat cgccgcgctc gatccacgtt caggatgttc
     1681 aggatcgtgg tccgcatctc ctcggccgag accggcatgg tgaggaagtc gcgcacaccg
     1741 gaaaccatcg ccttccgcat cgtgtcgcgg tcgccctggg tcgagactcc gactacgggc
     1801 caggcgcggg cgccggtcga gagcgcctcg accgtgcgga gcgagcgcgc caccggctcc
     1861 tcgatcgaga ccacgaccag gtcgacgtcg agctgctccg cccacgtgta ggcggccgct
     1921 ccgaaggtgc tctcgcccac gacgtcgatg tcgtcgaagc ggagcagatc aatcaggttg
     1981 tcgcgcgtct tctcgttgtc gtcgacgatc agaactctgg ttgctggcga ggtcattgct
     2041 cggactcccg gttacggtgg cggctggaca ctagttgccg tcgtcggtgg aggcggactg
     2101 gtccccggtt ggcgggaccg tcacggccga tacttcgatc cagccctccg tcttgccagc
     2161 gcggaccttg aaccaggcgc tcctggcctg gacgatctcg acggtgtcgc ctttcgcgac
     2221 ctcgccgaca acggcggcgg ttccgttcgg gtcggcgcgg agatcagccg aatcggcgct
     2281 gactgtcgcc gcatccgcct gaatgacggt cacgtccgcg acgttcacgc cgtcctgggt
     2341 gaccgtattc ggttcctgga tgatgccaag ccagtacttc gtggcggccg tcgcctcggt
     2401 caggcggaca gatccggtga tctctaccga ctggccgggc tcaaggttgc cgccgaagcc
     2461 ccagcggtac gggaatgggt tcggataggc gccggcgaag ctcaatccga tccggtaggt
     2521 gtcttccttc gggaagaagc cgagcgcgtc gtaggccacg ccctgggtgt actcgaatcc
     2581 cggtcgcgcg tccttgacgc tcttgatggt gtggtcggag acgttcttga ccgtcgcctt
     2641 gaactcgagg acgttgccag aggagatcac gcgctcggta aactcggcat gggtgatgat
     2701 cacgtcggac ggcaccatcg gcttgtcgac atcaccgagg aggtcgccga ccgggatgtt
     2761 gccgagctgg gccggcggca gattggcaat cgtcgtgtcg ccgggggccc gcatcgccag
     2821 tcgcaggctg cccttctcgt tcttgaccgt ctgggcggcc agcaggaggc gctgggcctg
     2881 ctcgggggtc accgcgagcg tcacgctctt ggcgccggga cgtgcaaccg gctctccaac
     2941 cgagaccgcg tcggttccat caacagtcgg cgtcggcgtg acggcctgtc cctcgacacc
     3001 gagctcttcc ggcgtcagcg cctgggagac agccagcacc tcgacgtcct gg
//
LOCUS       DASSGX010000018         3740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1308537, whole genome shotgun sequence.
ACCESSION   DASSGX010000018 DASSGX010000000
VERSION     DASSGX010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1308537"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2429
                     /locus_tag="VFI12_00260"
     CDS             <1..2429
                     /locus_tag="VFI12_00260"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET7054856.1"
                     /translation="SVVISKDLAADLKLTVGESFTAYVSGQPAELTVAAIAKNSALLG
                     FGSNPDGTTNNSAFAMPLPAAQSLLNHPGEINAIYLTQVGGVEQTRKAVESVHDKLVP
                     LLNGTGLGINPGKADFIAAAEGISSGFTSIFIVLGLFSIAAGILLIILIFTMLAAERR
                     PEMGMARAVGQRRLQLIQQFIAEGAGYALLAGIVGAALGVLATVLLAALLNSLLGDSF
                     PIRAKVTGTSLVSAYALGVFITFLTVVGASWKVSRLNIVAAVRDIPDVQRSKRRWRTL
                     IWASLLLLGGIALTLGGTSSGKAFPFYTGISLLPFGIALILIFFGVPGRLIFSLVGVY
                     LLFIWLIPDSVSSKIFGEYSGDIEMFFVSGIFLVIGSTILIVQNTSTLLRGVSALGGL
                     FKNRLASIKLAVAYPGQSRGRTGMAIAMFSLIIFALVMMATMSQNLTAAFLNKDALAG
                     WDVQADTNTGQPVADFPAKVAQAPINQSDIQVIGKLVHPAGYLAPMQQAGPDPGPAGG
                     YTIFEMDQTFLNTTKWKFQYRAAGYGSDQAVVDAMKSDSTLVVVDSIPITGNDFGPPA
                     SLELPAFKADGKSFQPIPISIANADGSGRTNVTVIGIIDPSLSYYQGMFGSGPTLDPV
                     IGNGGTERYYIKTAPGADTVAIARAIEGSVITSGVRVTSIQKALEDAQRITSGFLYMI
                     QGFMGLGLIVGLAAVGVIAFRSVVERRQQIGMLRAIGFQKAMVSTVFLVETAYVVILG
                     IIAGTIMGLILARNLLASDTEALDVQFHAPYGLITVILLGTIAIALLMAYVPSRQAST
                     ISPADALRYE"
     gene            complement(2420..3601)
                     /gene="hemW"
                     /locus_tag="VFI12_00265"
     CDS             complement(2420..3601)
                     /gene="hemW"
                     /locus_tag="VFI12_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HET7054857.1"
                     /translation="MAPLPDNAPLGIYLHVPFCTHICPYCDFNTYAGKSDLIPRYVEA
                     MARDLGIEADRLGRRPVTSIFFGGGTPSLLAPDHVQRLLGVIGDRFDLGPDVEASLEA
                     NPNGLTRDYLADLRSAGINRLSIGLQTTDRRGLRRLGRMHEAADAEQAVIAAVASGFQ
                     RLSLDLIFGWPGQTLDSWRNDLETVTSWAGGAVSHLSLYSLIVEPGTPMADAVQRGVV
                     SVLDDDATADLYELAMEFLGAKGWLHYEVANWARSEGQESVHNRIYWRNGEYLGIGAG
                     AHGRIGNRRVMRHLLPMTYITAIGAGDSAESNGEELTDEVQRGETMMLGLRLLREGVT
                     DVDFTRRHGRTLNEAFGPTIARFQTQGLLDRDDRGVRLTHRGLLLANDVCAAFLGGRY
                     S"
BASE COUNT          634 a         1260 c         1150 g          696 t
ORIGIN      
        1 gatcggtcgt gatcagcaag gatctggctg cggacctcaa attgaccgtc ggcgaatcct
       61 tcaccgccta tgtcagcggc caaccggcgg agttgaccgt cgcggcgatt gcgaagaaca
      121 gcgcgctcct tggcttcggc tcgaatccgg acggaacgac gaacaactcg gccttcgcaa
      181 tgcccttgcc cgccgcccaa tcgctgctca atcaccccgg tgagatcaac gccatttacc
      241 tgacccaggt cggcggcgtc gagcagacga ggaaagcggt cgaaagtgtc cacgacaagc
      301 tcgtcccgct cctgaatggc accgggctcg gcatcaatcc aggcaaagcc gacttcatcg
      361 ccgccgccga gggaatcagc tcaggcttca cgagcatctt catcgtgctc ggcctcttct
      421 cgatcgcggc cggcatcctg ctgatcatcc ttatcttcac gatgctcgcg gctgagcgcc
      481 ggccggagat gggcatggcc cgcgcggtcg gccagcgccg gctgcaactg atccagcaat
      541 tcatcgccga gggcgccggg tacgcgctcc tggccggcat cgtcggcgcc gcacttggcg
      601 tgctggcgac ggtcctgctg gccgcgctcc tcaactcgct tctgggcgac tcattcccga
      661 tccgggccaa ggtcaccggc acgtctctgg tttcggccta tgcgctcggc gtcttcatca
      721 ccttcctgac ggtcgttggg gcctcgtgga aagtgagccg gctcaacatc gtcgcggccg
      781 tgcgagacat cccggacgtt cagcgctcga agcgtcgctg gcgaaccctg atctgggcct
      841 ccctgctttt gcttggggga atcgccctca cgcttggcgg caccagctcc ggcaaggcct
      901 tcccgttcta caccggcatc agcctgcttc cgttcggcat cgcgctgatc ctgatcttct
      961 tcggcgtgcc gggccgcctg atcttctcgc tcgtcggtgt gtacctgctc ttcatttggc
     1021 tgatcccgga tagcgtgtcc tcgaagatct tcggcgagta cagcggcgac atcgagatgt
     1081 tcttcgtgtc gggcatcttc ctcgtgatcg gctcgacgat cctgatcgtc cagaatacga
     1141 gcacgctcct gcgcggcgtg tcggcgctgg gcggtctctt caagaaccgg ctggcatcga
     1201 tcaagcttgc cgtcgcctac ccgggccagt cgcgcggccg gaccggcatg gcgatcgcca
     1261 tgttcagcct gatcatcttc gcgctggtga tgatggcgac catgtcccag aacctgaccg
     1321 ccgccttcct caacaaggac gcgctcgccg gctgggacgt ccaggccgac accaacaccg
     1381 ggcagccggt cgcggatttc ccggccaagg tcgcccaggc gccgatcaat cagtcggaca
     1441 tccaggtcat cggcaagctg gttcacccgg ccgggtatct cgcgccgatg cagcaggccg
     1501 ggccggatcc cgggccggcc ggcggttaca ccatcttcga gatggatcag acgttcctca
     1561 atacgacgaa gtggaagttc cagtaccgcg ccgccggcta tggctcagat caggctgtcg
     1621 tcgacgccat gaaatcggac tccacgctgg tcgtggtcga ttcgatcccg atcaccggaa
     1681 acgacttcgg gccgccggcg tcgctcgagc taccggcgtt taaagcggac gggaagtcgt
     1741 tccagccgat tccgatctcg atcgcgaacg cggatggcag cggccgcacc aacgtgaccg
     1801 tcatcggcat catcgatccg tccctctcct actaccaggg gatgttcgga tccgggccga
     1861 ccctggatcc ggtgatcggc aacggcggca ccgagcgcta ctacatcaag acagcaccgg
     1921 gcgctgacac ggtcgcgatc gcgcgggcga tcgaaggcag cgtcatcacg agcggtgtcc
     1981 gcgtgacttc gatccagaag gcgctcgagg atgcccagcg gatcacgagc ggcttcctct
     2041 acatgatcca gggcttcatg ggcctcggcc tgatcgttgg attggcggcc gtcggcgtga
     2101 tcgcattccg gtcggttgtc gagcgacgcc agcagatcgg catgctccgg gcgatcggct
     2161 tccagaaggc gatggtgtcg accgtcttcc tggtcgagac cgcctatgtc gtcatcctcg
     2221 gcatcatcgc aggtacgatc atgggcctga tcctggccag gaacctgctc gcgtcggaca
     2281 ccgaggcgct ggacgtccag ttccatgcgc cgtacgggct gatcaccgtc atcctgctcg
     2341 gcacgattgc gatcgcgctg ctgatggcgt acgtgccatc gcggcaggca tcgacgatct
     2401 cgccggccga cgcgctccgc tacgagtagc gtccgccgag gaatgccgcg cagacgtcgt
     2461 tcgcgagcaa taacccacga tgcgtcaggc ggactcctcg atcatcccga tcgaggagtc
     2521 cctgcgtctg aaaccgcgcg atcgtcgggc caaatgcctc attcagcgtc cgcccgtgcc
     2581 gtcgcgtgaa gtcgacatcg gtcacgcctt cgcggagcag ccggagaccc agcatcatcg
     2641 tctcgccccg ttgcacttca tcggtcagct cttcgccgtt cgactcggct gaatccccgg
     2701 ccccgatcgc ggtgatgtag gtcatcggca acaggtgacg catcacccgg cgattgccga
     2761 tccggccgtg tgctccggcc ccaatcccga ggtactcgcc gttacgccag tagatccggt
     2821 tgtggaccga ctcctgcccc tcggaccggg cccagttggc cacctcgtag tggagccagc
     2881 ctttcgctcc caggaactcc attgccagct catacaggtc ggcagtggcg tcatcgtcga
     2941 gtaccgagac gacgccccgc tgcacggcat ccgccatcgg cgttcccggc tcgacgatca
     3001 gcgagtagag cgacaggtgc gagacggccc cgccggccca cgacgtgacc gtctccaggt
     3061 cgtttcgcca ggaatcaagc gtctgtccgg gccagccgaa gatcaggtcc aggctgagcc
     3121 gctggaatcc ggaagcaacg gccgcgatta ccgcctgttc ggcatccgcc gcctcgtgca
     3181 tccggccgag gcggcgcaat ccgcgccggt cggtggtttg caatccgatg ctgaggcggt
     3241 tgatgccggc cgagcgaagg tcagccaaat agtcgcgggt caaaccgttc ggattcgcct
     3301 cgaggctggc ctcgacatcc ggcccgaggt caaatcgatc cccgatgacc ccgagtagcc
     3361 gctgtacatg atctggagcc agcagcgatg gcgttccgcc tccgaagaaa atcgacgtga
     3421 ctggccggcg accgagccgg tccgcttcga tcccgaggtc gcgagccatc gcctcgacat
     3481 agcgcggtat caggtccgac ttcccggcgt aggtattgaa atcgcagtac gggcagatat
     3541 gggtgcagaa cggcacgtgc aggtagatgc caaggggggc gttgtcgggg agcggggcca
     3601 cggtgagatc ctgatcagcg ggacagtcct gtcgtcacgg accagtctag gcggaatcgc
     3661 gctgttcgtc cgccgattcc agcttcacgt gtttcaagcg caggtaatcg gtcatcccca
     3721 caacttccga ctgcgacccg
//
LOCUS       DASSGX010000019         2003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1090853, whole genome shotgun sequence.
ACCESSION   DASSGX010000019 DASSGX010000000
VERSION     DASSGX010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090853"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..860
                     /locus_tag="VFI12_00270"
     CDS             <1..860
                     /locus_tag="VFI12_00270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7054858.1"
                     /translation="EVFGLAGESGCGKSTVALALLRLLQPPAIITGGDVHFQGHDILA
                     MNDVQLQRFRWDQISMVFQSAMNALNPVMTVGEQLIDAIRAHDPGRSRKAARERAKEL
                     LEIVGIDAERVDAYPHEFSGGMRQRAMIGMALALDPPMMILDEPTTALDVVVQKEIMQ
                     QIEDLKKNLGFSILFITHDLSLLVEFSDRIAIMYAGQIVELAPAAELFANPLHPYTMG
                     LMSSFPTLTGDKKRLTGIPGSPPDMACPPSGCRFHPRCPKFQPRHAEVVPELREITPG
                     HWVACHLYT"
     gene            869..1873
                     /locus_tag="VFI12_00275"
     CDS             869..1873
                     /locus_tag="VFI12_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7054859.1"
                     /translation="MADPVPTTEVPLLDVRHLSKQFPIGGLIHSINLHALRDVSFSVK
                     RGEVISVVGESGSGKSTTARAVARLIAPTAGEIVFNGVDMLKKEPRRPSLQYRGEVQM
                     VFQDPFGSLNPVHSIGHHLTRPLQIHKKARGRGETREAMHELLRTVGLEPAATYASKF
                     PHQLSGGQRQRVAIARALAPDPKLLLADEPISMLDVSIRMGILNLMERLKHDRGLAYL
                     YITHDLASARYIGDRTHVMYAGRMVEGAPSEELVSRPAHPYTKLLLSAVPDPRGERKR
                     VEARGEVPSLVEPPPGCPFAARCPHVMPVCREVMPGAVDVAPGHWVRCHLYGSGAAHA
                     "
BASE COUNT          329 a          705 c          620 g          349 t
ORIGIN      
        1 gcgaggtctt tggtctggcc ggcgaatccg gctgcggcaa gtcgacggtc gccctcgcgc
       61 tgctgcggtt gctgcagccc ccggctatca tcaccggcgg tgatgtccac ttccagggtc
      121 acgacatcct cgcgatgaac gacgtccaac tgcaacgctt tcgctgggac cagatttcga
      181 tggtcttcca gagcgccatg aacgcgctca atccggtcat gacggtcggc gagcaactga
      241 tcgatgcgat ccgcgcgcac gatccgggcc gctcgcgcaa ggctgcccgg gaacgcgcga
      301 aagagctgct cgagatcgtc ggcatcgacg ccgagcgcgt ggatgcctac ccgcacgagt
      361 tctccggcgg tatgcgccaa cgcgccatga tcggcatggc gctcgccctg gacccgccga
      421 tgatgatcct cgacgagccg acgaccgccc tcgacgtggt cgtgcaaaag gagatcatgc
      481 agcagatcga ggatctcaag aagaaccttg ggttctcgat cctcttcatc acccacgacc
      541 tttcgctgct ggtcgagttt tccgaccgga tcgcgatcat gtacgccggc cagattgtcg
      601 agctggcccc ggccgcggag ttgttcgcca atcccctgca cccctacacg atggggctga
      661 tgtcctcgtt cccgaccctg accggcgaca agaagcgcct gaccgggata cccggcagcc
      721 cgccggatat ggcctgcccg ccctccgggt gtcgcttcca tccccgctgc ccgaagttcc
      781 agccacggca tgcggaggtg gtgccggaac tccgcgagat cacacccggg cactgggtcg
      841 cgtgccacct ctatacatag gaagcgccat ggccgatcca gtccccacaa ccgaagttcc
      901 gctgctcgac gtccggcacc tgtccaagca attcccgatc ggcgggctga tccactcgat
      961 caacctgcac gcgctccggg atgtctcgtt cagcgtgaag cgaggcgaag tcatctccgt
     1021 cgtcggcgaa tccggcagcg gcaagagcac gaccgcccgg gccgtcgccc gcctgatcgc
     1081 accgaccgcg ggcgaaatcg tcttcaacgg cgtggacatg ctcaagaagg agccgcgccg
     1141 accgtccctg cagtaccgcg gcgaggtcca gatggtcttc caggacccat tcggctcgct
     1201 caaccccgtc cattcgatcg gccatcacct gactcgcccg ctccagatcc ataagaaggc
     1261 gcgcggccgg ggcgagacgc gggaggcaat gcacgagctg ctccggaccg ttggactgga
     1321 accggccgcg acctacgcct ccaaattccc gcatcaactc tcgggcgggc agcgccagcg
     1381 tgtcgccatc gcccgcgccc tggcgcctga cccgaagttg ctcctggcgg atgagccgat
     1441 ttcgatgctc gacgtctcga tccggatggg cattctcaac ctgatggagc ggctcaagca
     1501 tgatcgtggc ctcgcctacc tctacatcac ccacgacctc gccagcgctc gctatatcgg
     1561 ggatcgcacg cacgtcatgt acgccgggcg gatggtcgag ggcgcgccga gcgaggagct
     1621 tgtctcgcgg ccggcccacc cttacacgaa gttgctgctc tcggccgtgc cggacccgcg
     1681 aggggagcgg aaacgggtcg aggcccgggg tgaagttccg tcgctggtcg agccgccgcc
     1741 gggctgcccc ttcgccgccc gctgcccgca cgtgatgccg gtctgccgcg aggtgatgcc
     1801 cggcgcggtc gatgtcgcgc ccggccactg ggtgcggtgc cacctctacg gctccggcgc
     1861 cgcgcatgcc tgaacgccat tggattgttg atatgagagg agaccgttcg tgacgatcgc
     1921 gcttggtatt gtcggttgcg gagatgtcgc cttccgcacc tatttcccgg gcttcgcccc
     1981 gatcgtcgcc gatggctccg ctc
//
LOCUS       DASSGX010000020         3598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1417715, whole genome shotgun sequence.
ACCESSION   DASSGX010000020 DASSGX010000000
VERSION     DASSGX010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1417715"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..279
                     /locus_tag="VFI12_00280"
     CDS             85..279
                     /locus_tag="VFI12_00280"
                     /inference="COORDINATES: protein motif:HMM:NF016828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HET7054860.1"
                     /translation="MLQFVGSYISTLLSKFRNDDEEGQTLVEYGLLLALIAIIVIVAL
                     IFLGPIVSRIFQNVGTSLQV"
     gene            528..1397
                     /gene="cpaB"
                     /locus_tag="VFI12_00285"
     CDS             528..1397
                     /gene="cpaB"
                     /locus_tag="VFI12_00285"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HET7054861.1"
                     /translation="MILVGVLLAVLAFIAIVVFLNQGPGAPQQEVQATVLVAKEDIAI
                     GQPVTPDMVQTKLVPPTGVSGTALTDPSQVGGRPAVVAVPAGAQVSKETFGEGLLVVN
                     IPGQLKPGEKAVTLQLDRTTGADFLVKPGDVIDVVIAQDVQVLQPSSDSTAQNPRFEP
                     VTGLSAARTVKTVLQNKRVLYVSQSRATQTAATPSPGAEQPAAAQPQLTDTVLIVFAG
                     TDQDAELIKFAQRDAVEASGARLFSVTIRATQDTDNERTTGITIDRLVDDYGLPVPGI
                     VEQVGGGKTKPGR"
     gene            1426..2646
                     /locus_tag="VFI12_00290"
     CDS             1426..2646
                     /locus_tag="VFI12_00290"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7054862.1"
                     /translation="MIKVLIVDDIPETRDHLTKLLATEQEIDVVATAGSGEEAIRLAM
                     EVRPDVIVMDINMPGMDGVAAAEFISQRLPTSPIIMMSVHGEAEQLRRAIAAGAREFL
                     VKPFSADEFSTTIHSVFGKELARREQLQVVIPPTPGGQSENIEDHQVIAMFSPKGGAG
                     RTTIATNLALAIQRETKSRVCLIDANLQFGDIGVLLNLNPKNRSMLDAVEGGEPDADI
                     INSVVIDHSTGIRVLLAPPAPEGADLVTPAYLRKMVDQLRVNHDYVIVDLPAALTDHS
                     LGVMDAADQIVVLSALEITTIKNVRLFLEVADQLEYDRSKIRLVINRSDTAQGIRIGD
                     VEASIRRSIDGTIVSDGRLAVLAVNRGVPFVVSHPDSALSRDIAKLARTLAGEPAANK
                     STEKSAKRGLFARR"
     gene            2663..>3598
                     /locus_tag="VFI12_00295"
     CDS             2663..>3598
                     /locus_tag="VFI12_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HET7054863.1"
                     /translation="MSLLKRIERVNRPAGEGEPETPSSSPNVPATTTPTAPAGPPSPP
                     AGNGARPPIAAAPSAPTTTSRPFLGNLPARESFREAKFRVQNRLINELDPKLDLTNQV
                     EVRRQIEDLFGKIADEEGLALTRAERVRMLEQITDEILGLGPLEPLLRDETLTEVMVN
                     GPQQVYVEREGRLELTNVTFQNDEHVMKIIQRIIAPIGRRVDESSPMVDARLADGSRV
                     NAIIPPLSLVGPVITIRKFSATPYTVDDLVRFGTATPEMFEFLEACVKARLNVFVSGG
                     TGSGKTTMLNILSSFIPDDERIVTIEDAAELQLRQE"
BASE COUNT          647 a         1239 c         1101 g          611 t
ORIGIN      
        1 ggataggacc atgaggccat acacgacctc tgcagcgttc cgtacgctgc ccgcaaatct
       61 tcgaggaaag gaggttttgc caatatgctt cagttcgtcg gttcttacat cagcactctt
      121 ctctcaaagt tccgtaacga cgacgaggaa ggccagaccc tggttgagta cgggcttctg
      181 ctcgccctca tcgcgatcat cgtgatcgtg gccctgatct tccttggccc gatcgtgagc
      241 cggatcttcc agaacgtcgg caccagcctg caggtctaac ctcaggtcgt ttcgacacca
      301 cagagccccg cgtcaactcg gcgcggggct ctgtcatgcc cgggtcgtgt cgggcatttc
      361 tgggcgccgg gtagtacttc ggcatagccc acaagtccgc tcgaacgctg ctggacactg
      421 cacctagact gttgaccgca gtcacaacgg ctgcatcatt ccctggcctt atcgaccacc
      481 cattccgagc tctaaggggt acatctgtga agcggtctaa tcgcctggtg atcctggttg
      541 gtgtgctgct cgccgtcctc gcgttcatcg cgattgttgt cttcttgaac caggggccgg
      601 gcgctccgca gcaggaagtg caggccaccg tcctggtcgc caaggaagac atcgcgatcg
      661 ggcagccggt cacgcctgac atggtgcaga cgaagctcgt cccgccgacc ggcgtctcgg
      721 gtaccgccct caccgatccc tcgcaggtcg gcggccgtcc cgccgttgtg gccgttccgg
      781 ctggggccca ggtgagcaag gaaacgttcg gcgaaggact gctcgtcgtg aatatccccg
      841 ggcagctgaa gcccggcgag aaggccgtga cgctgcagct cgaccgcacg accggcgccg
      901 acttcctggt gaagccaggg gacgtgatcg acgttgtcat cgctcaggac gtgcaagtcc
      961 tgcagccgtc gtccgactca accgctcaga atccgcgttt cgagccggtc accgggctgt
     1021 cggccgcgcg caccgtgaag acggtgctcc agaacaagcg cgtgctgtac gtgagccagt
     1081 cgcgggctac ccagaccgcg gcaacgccaa gtccgggagc agagcagccc gctgccgctc
     1141 agccgcagct gaccgatacc gtgctgatcg tcttcgccgg caccgaccag gacgccgagc
     1201 tgatcaagtt cgctcagcgc gatgcggtcg aggcatccgg ggctcgcctg ttctcggtca
     1261 cgatccgcgc gacccaggac acggataacg agcgcacgac cggtatcacc atcgaccgcc
     1321 tggtcgacga ctatggcctc ccggtgccgg gcatcgtgga gcaggtcggc ggcggcaaga
     1381 ccaagccggg tcgctgagca accccctgac gctggagtcc tgaccgtgat caaggtcctg
     1441 atcgtcgacg acatccccga gacgcgtgac cacttgacca agctcctcgc caccgagcag
     1501 gagatcgacg tggtggccac cgccggctcc ggggaggagg cgatcaggct cgccatggag
     1561 gtgcgccccg acgtcatcgt catggacatc aacatgccgg gcatggatgg ggtggccgcc
     1621 gccgagttca tctcgcagcg gctgcccacc tcgccgatca tcatgatgag cgtccacggc
     1681 gaggccgagc agctccgtcg cgccattgcg gccggggctc gcgagttcct cgtcaagccg
     1741 ttctcggccg atgagttcag caccaccatc cacagcgtct tcggcaagga gctggcgcgc
     1801 cgcgagcagc tgcaggtcgt cattccgccc accccgggcg gccagtccga gaacatcgag
     1861 gaccatcagg tcatcgccat gttctcgccg aagggcggcg ccggccgaac gacgatcgcc
     1921 accaacctgg cgctggccat tcagcgcgag acgaagagcc gggtgtgcct gatcgacgcg
     1981 aacctgcagt tcggcgacat cggtgtattg ctcaacctca accccaagaa tcgctcgatg
     2041 ctcgatgcgg tcgaaggtgg cgagccggat gcggacatca tcaattcggt ggtgatcgac
     2101 cattccacgg gaatccgggt gctgctcgcg cctccggcgc ctgagggagc ggacctcgtg
     2161 actcccgcct acctgcggaa gatggtcgat cagctccgag tcaaccacga ctacgtcatc
     2221 gtcgacctgc ctgcggcgct gaccgaccac agcctggggg tcatggacgc ggccgatcag
     2281 atcgtagtgc tctccgctct cgagatcacc accatcaaga acgtccgcct gttcctggag
     2341 gttgccgacc aactcgagta cgatcggtcg aagattcgtc tcgtgatcaa ccgctccgac
     2401 actgcccagg ggatccggat cggcgacgtg gaggcttcca tccgccgctc gatcgacggc
     2461 accatcgttt ccgatggccg ccttgccgtg ctcgccgtga atcgcggcgt tccgttcgtc
     2521 gtatcccacc cggatagcgc gctgtcgcgc gatatcgcca agctcgctcg tacgctcgcg
     2581 ggagagcccg ccgccaacaa gtccacagag aagtcagcca agcgcggact cttcgcacgc
     2641 cgctaagggg aaggaactca ccatgtcatt gctgaagcga atcgagcgag tcaaccgacc
     2701 cgctggagaa ggggagcccg agacgccgtc gtcgtcgccc aacgtgccgg ccacgacgac
     2761 gccgactgct cccgccggac cgccctcgcc gcccgccggc aacggcgcgc gtccgccgat
     2821 cgcggctgcg ccatcggctc cgacgacgac cagccggccg ttcctgggca accttcccgc
     2881 gcgcgagtcg ttccgcgagg ccaagttccg tgtccagaac cgcctgatca acgagctcga
     2941 cccgaagctc gacctgacca accaggtcga ggtgcgtcgc cagatcgagg acctcttcgg
     3001 caagatcgcc gacgaggagg gtctggccct gacccgcgcc gagcgggtgc ggatgctcga
     3061 gcagatcacc gacgagatcc tcggcctcgg gccgctcgag ccgctgctcc gtgacgagac
     3121 cctcaccgaa gtgatggtca acggaccaca gcaggtctac gtcgagcgcg aaggccgcct
     3181 ggagctgacc aacgtcacgt tccagaacga cgagcacgtg atgaagatca tccagcgcat
     3241 cattgcgccg atcggacgtc gcgtcgacga gtcgagcccc atggtcgacg cccgcctggc
     3301 agatggttcc cgcgtgaacg cgatcattcc gccgctctcg ctggtcggcc cggtcatcac
     3361 cattcgaaag ttctcggcca cgccgtacac cgtggacgac ctcgtccgct tcggcaccgc
     3421 caccccggag atgttcgagt tcctggaggc ctgcgtgaag gctcgcctca atgtcttcgt
     3481 ctcgggcggc accggctccg gcaagacgac gatgctcaac atcctctcgt ccttcattcc
     3541 tgacgacgag cgaatcgtca ccatcgagga cgccgccgag cttcagctcc ggcaggag
//
LOCUS       DASSGX010000021         2242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1090994, whole genome shotgun sequence.
ACCESSION   DASSGX010000021 DASSGX010000000
VERSION     DASSGX010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090994"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VFI12_00300"
     CDS             complement(<1..244)
                     /locus_tag="VFI12_00300"
                     /inference="COORDINATES: protein motif:HMM:NF024843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HET7054864.1"
                     /translation="MGDQIRTCRNCGAVFPFTEGEQAFYLERGYQPPAYCPDCRDLRR
                     ATARQSDLAPRGVHRQLFAAICSECGAPTEVPFQPRP"
     gene            412..1545
                     /gene="sucC"
                     /locus_tag="VFI12_00305"
     CDS             412..1545
                     /gene="sucC"
                     /locus_tag="VFI12_00305"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HET7054865.1"
                     /translation="MNIHEYQAADLLARYGIPTNPGSVATTPDEVAQAAKAAGGTVVV
                     KAQVHTGGRGKAGGVKLAKSPDEARAVGEQILGMDIRGHTVHKVLVAPGVSIAKEFYL
                     GVVLDRPNRKLVVMASAEGGVEIEIVAKERPDKIKTASVDPYLGLHAYQARQLGFELG
                     LEADKVGGFADIVLKLAKLYQELDATLAEINPLILTGDGTWMALDSKVSFDDNALFRH
                     SQFEELRDMNEEQATEVEARRSGISFVKLDGNIGCIVNGAGLAMATMDAVKLHGGEPA
                     NFLDVGGGASADQVAKAFSLVTADPNVKAILINIFGGITRGDIVAQGITDAMSRVGVT
                     VPIVVRLSGTNAEAGREILDANGLHAVATMDEAAAKVVAEAAA"
     gene            1577..>2242
                     /gene="sucD"
                     /locus_tag="VFI12_00310"
     CDS             1577..>2242
                     /gene="sucD"
                     /locus_tag="VFI12_00310"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HET7054866.1"
                     /translation="MSILVDKSTRLLVQGITGREGSFHTTQMVEYGTNVVAGVTPGGR
                     GKTVADAPVFNTVAEAVAETQPNVSIIYVPAKFAPDAIYEAVDNGIPFVICITEGIPV
                     MDMVPVYAYVKAKGARLLGPNCPGIITPGQAKVGIMPGFIHTPGPVGLVSRSGTLTYE
                     VVDALTKAGLGQTTAIGIGGDPIIGTSFVDALALFQADPETTAIVMIGEIGGNDEETA
                     AEYI"
BASE COUNT          429 a          698 c          760 g          355 t
ORIGIN      
        1 ccggtcgtgg ctggaacggg acctcggtcg gcgcgccgca ctcggagcaa atggcggcga
       61 agagctggcg atgcacgccg cgcggcgcca gatcgctctg gcgggccgtg gcccggcgga
      121 ggtcccggca gtccgggcaa taggcgggtg gctggtagcc gcgctccagg tagaaagcct
      181 gttcgccctc cgtaaatgga aatactgccc cgcagttccg gcaggtgcgg atctggtcgc
      241 ccacggaaga caactccggt tcaccgtcga acccggctcg gcgtacgtcg catcgcgagt
      301 tgattcgcat caagccgtac tgcgcattgt gctacattgc ggatcgttat tggcggcacg
      361 gcgcaccatt tgcccgccag caggccacca ttccgaggag agcccagcag catgaatatc
      421 cacgagtacc aggcggcaga tttgctcgcc cggtatggga tccccacgaa tccgggaagt
      481 gtcgcgacga cgccagacga agtcgcgcag gccgcgaagg cggccggcgg cacggttgtc
      541 gtcaaggcgc aggtccacac cggcggtcgc ggcaaggccg gcggcgtcaa gctggcgaag
      601 tcgccggacg aagcgcgagc cgtgggtgag cagatcctgg ggatggatat tcgcggacat
      661 accgtgcaca aggtgctggt tgcgccgggc gtctcgatcg cgaaggagtt ctacctcggc
      721 gtcgtgctcg atcgcccgaa tcggaagctg gtcgtgatgg cttcggccga gggcggcgtc
      781 gagattgaga tcgtggccaa ggagaggccg gacaagatca agacggcctc tgtcgaccca
      841 taccttggcc ttcatgcata ccaggcgcgc cagctcggct tcgagctcgg actggaagcg
      901 gacaaggtcg gcggattcgc cgacatcgtc ctcaagctcg ccaagctcta ccaggagctg
      961 gatgcgacgc ttgcggagat caacccgcta atcctgaccg gtgacggtac atggatggca
     1021 ctggactcca aggtcagctt tgacgacaac gccctcttcc ggcactcgca gttcgaggag
     1081 ctacgagaca tgaatgagga gcaggccacc gaggtcgaag cccgccgttc cgggatctcc
     1141 ttcgtcaagc tcgatggcaa catcggctgc atcgtcaacg gcgccgggct ggcaatggcg
     1201 acaatggacg ccgtcaagct ccacggcggc gagccggcca acttcctcga cgtcggcggt
     1261 ggcgccagcg ccgaccaggt ggcgaaggcg ttcagcctgg tcaccgccga cccgaacgtc
     1321 aaggcaatcc tgatcaacat cttcggcggc atcacccggg gcgacatcgt cgcgcagggc
     1381 atcaccgacg cgatgagccg ggtcggtgtc acggtcccga tcgtggttcg cctctcgggc
     1441 acgaacgcgg aagcgggacg tgagattctg gatgcgaacg gcctgcacgc cgtcgcgacg
     1501 atggacgaag cagcggcgaa ggtcgttgcc gaggcagccg catagcgacg gtcgggattg
     1561 accggggagc agatttgtga gcattctcgt cgacaagagc acgcggctac tggtgcaagg
     1621 catcaccggc cgcgagggat cgttccacac cacgcagatg gtcgaatacg gcacgaacgt
     1681 ggtcgccggc gtcaccccgg gcggccgggg caaaaccgtt gccgatgcgc cggtctttaa
     1741 tacggtggcc gaggcggtcg cagagacgca gccgaacgtc tcgatcatct atgtaccggc
     1801 caagttcgcg cccgacgcga tctatgaggc ggtcgacaac ggcatcccat tcgtgatctg
     1861 catcaccgag ggcataccgg taatggacat ggtgccggtc tacgcctacg tcaaggcgaa
     1921 gggcgcgcgg ttgctcggcc cgaactgtcc cggcatcatc accccggggc aagccaaagt
     1981 cggaatcatg cccggcttca tccacacgcc ggggccggtc gggcttgtct cgcggtccgg
     2041 cacgttgacc tacgaggtcg tggacgcgct gacgaaggcc ggtctcggcc agacgacggc
     2101 gatcggaatc ggcggcgatc cgatcatcgg cacctcattc gtggatgcgt tggccctttt
     2161 ccaggccgac cccgagacga cggcgatcgt gatgatcggc gagatcggcg gaaacgacga
     2221 ggagacagca gccgaataca tc
//
LOCUS       DASSGX010000022         5963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_112018, whole genome shotgun sequence.
ACCESSION   DASSGX010000022 DASSGX010000000
VERSION     DASSGX010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5963
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112018"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..157)
                     /locus_tag="VFI12_00315"
     CDS             complement(<1..157)
                     /locus_tag="VFI12_00315"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET7054867.1"
                     /translation="MRILITGGAGYIGSVAVEQSLAAGHEVVVVDNLWRGHRAAIPDE
                     VEFRECDL"
     gene            271..1527
                     /gene="glyA"
                     /locus_tag="VFI12_00320"
     CDS             271..1527
                     /gene="glyA"
                     /locus_tag="VFI12_00320"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HET7054868.1"
                     /translation="MVTTTDVLTDPEVAASIRREEIRQRDGIELIASENFVSRNVMAA
                     VGSVLTNKYAEGYPGRRYYGGCEFIDEIETLAIERAKQLFGVEHVNVQPHSGANANLG
                     AYLAIAEPGSKILGLSLSEGGHLTHGHPVNFSGRLFEAHFYTLDHATGRIDYDNAEKV
                     AKEIRPAIIIAGASAYSRHIDYARFRQIADEVGAYLVADIAHPAGLVAAGLHPSPVGH
                     AHITTTTTHKTLRGPRGGMIMTSEELGPAVDKSVFPGMQGGPLEHVIAGKAVAFREAL
                     QPSFRAYAAAVIENAQALSDELASLGMSIVSGGTDTHLMLVDITPTGMSGKKAERILD
                     SVGITANKNAIPNDPRPPMQTSGIRLGSPAVTTRGYGVAEMRQVARMIVDTLANPEDE
                     AVLSRVRDDVAELSRRFPHPGVDSSI"
     gene            1549..2649
                     /locus_tag="VFI12_00325"
     CDS             1549..2649
                     /locus_tag="VFI12_00325"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HET7054869.1"
                     /translation="MDRPLIAAATFLTALAISAILVRQTIPFARARGLLDQVGGRRVN
                     PNRLPRIAGPAILIAFLAGMTMTWILGVDRFADERNRILLLIPASILLVGVMIVDDIR
                     GIRPLPKLLLQIGVALIVVGPYAFDRTWGIAINQLNLPVQGTVQFGPAVAIVFSLFWV
                     VGMMNTINLIDGLDGLAGSLTLVACAILFLHTYFWPRDDPQFTISLLPAALGGAVLGF
                     LLYNWHPARIILGDAGAYFLGLALAVTAIIGGAKIATTLLALGLPILDVAWVAISRVQ
                     RGSSVVQADRGHLHHRLLDLGWSQARIVASVAGASLAFGIASLLLPNRELKLAAIAIV
                     GLVLIGTVVIVATVDEDGNRKLGTRLPGARSR"
     gene            complement(2910..3101)
                     /locus_tag="VFI12_00330"
     CDS             complement(2910..3101)
                     /locus_tag="VFI12_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7054870.1"
                     /translation="MNEQQQSNSSVVVFLAGVVVGGVGGGIAGWLLGGHVAPLLTSLI
                     DLLDRDSKTQTVRFEALQQ"
     gene            complement(3123..3470)
                     /gene="rsfS"
                     /locus_tag="VFI12_00335"
     CDS             complement(3123..3470)
                     /gene="rsfS"
                     /locus_tag="VFI12_00335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HET7054871.1"
                     /translation="MAEARALALHIADILTETPASDTVVLDISGRSSFADFFVICSGE
                     NERQLRAVANAVREHLSDEGRLPRRSEGEFISGWIVLDYADVVVHIFDVDQRDFYRLE
                     ELWSDAVTVLAIQ"
     gene            complement(3553..3627)
                     /locus_tag="VFI12_00340"
     tRNA            complement(3553..3627)
                     /locus_tag="VFI12_00340"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3593..3595),aa:Ile,seq:cat)
     gene            complement(3688..4605)
                     /locus_tag="VFI12_00345"
     CDS             complement(3688..4605)
                     /locus_tag="VFI12_00345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET7054872.1"
                     /translation="MDTAHTQLHRFAWISIAAAVITIVLKFAAWWVTDSVGLLSDALE
                     SCVNLVGAIAALIALWYAGQEPDEEHAYGHAKAEYFSSGLEGMLILIAAVSIIYSAAQ
                     RLQSPAEVESAGLGVSIAVAASIVNLIAARWIQRAGEHHDSIALEAGARHLLTDVLTS
                     AGVIIGIVLIQFTGWTRIDPIVALLVAANIIRVGFDLMKRSMLGLLDTALPPDELGNI
                     ERVLEQYRQPGKIEMHALRTRQAGQRRFGSVHILVPGNWTVHNGHHLVECIERDIRAA
                     VPGIVMITHLESLDDPRSWDDKRLPPLGE"
     gene            complement(4664..5500)
                     /gene="panC"
                     /locus_tag="VFI12_00350"
     CDS             complement(4664..5500)
                     /gene="panC"
                     /locus_tag="VFI12_00350"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF014611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HET7054873.1"
                     /translation="MTSGRIVTTPAQLRAAMGGQPVGLVPTMGALHEGHLSLIRQSAR
                     DNPLTVVSVFVNPTQFGSATDLAAYPRVLESDAARAFTVGADIIYAPAAEIVYPPGFS
                     TTVTVAALTDRWEGASRPGHFAGVATVVAILLNTVRPARSYFGEKDFQQLAVIRRMHA
                     DLLLPGEIIGRPTVRDRDGLALSSRNARLSPDERAAAAAIPQALYTMRDAADAGETDS
                     GRLVARGRMIIDAQPLLTLDYLAIVDEQTLEPLGVLRSGSRALVAVVARDTRLIDNLA
                     IA"
     gene            complement(5497..>5963)
                     /locus_tag="VFI12_00355"
     CDS             complement(5497..>5963)
                     /locus_tag="VFI12_00355"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF014593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HET7054874.1"
                     /translation="ITRIGIPVVAHIGLLPQTAGLDGGYRVQGKDEAAVRQLMADAQA
                     VVAAGAFAVVIELVGAELAGRITASTPIPTIGIGAGVECDGQVLVAPDMLGMDERFRP
                     RFLKTYSDLSGTITAAFSAYASDVRTRAYPDAAHSFKTPPAALAVLDEDDIE"
BASE COUNT         1032 a         1975 c         1916 g         1040 t
ORIGIN      
        1 gcagatcgca ctcgcggaac tcgacctcgt ccgggatcgc cgcccggtgc ccgcgccaaa
       61 ggttatccac caccacgacc tcgtgcccgg cggcgagcga ctgctcgacc gcgacgctcc
      121 cgatgtaccc cgcgccgccc gtaatcagaa ttcgcacgta tccctcgctg cctgcaacat
      181 cggccgaatg ccgctgtctg gatggtagac tagcgcgtaa tctacaccct tcatttcgcg
      241 cccgttccca cgcagaaaag gactcccggc atggttacga ctactgacgt tttgaccgat
      301 ccggaggtcg ccgcttcgat ccggcgggaa gagatccggc agcgtgacgg catcgaattg
      361 atcgcctccg agaacttcgt cagccgcaac gtcatggcgg cggtcggctc ggtcctgacc
      421 aacaagtacg ccgaaggcta ccccggacgg cgctattacg gcggctgcga gttcatcgac
      481 gagatcgaga ctctggcgat cgagcgtgcg aagcaactct tcggcgtcga gcacgtcaat
      541 gtccagccgc actcgggcgc gaatgcgaac ctcggcgcat acctcgccat cgccgagccg
      601 ggtagcaaga ttctcggact cagcctttcc gaaggcgggc acctgactca tggccacccc
      661 gtcaacttca gcgggcggct cttcgaggct catttctaca cgctggatca cgcaaccggc
      721 cggatcgact acgacaacgc ggagaaggtg gcgaaggaga ttcgcccggc gatcatcatc
      781 gccggcgcca gcgcatacag ccgccacatc gattacgccc gcttccgcca gatcgcggac
      841 gaggtcggcg cgtacctggt cgctgatatc gcgcacccgg ccggtctcgt tgccgccgga
      901 ctgcacccgt cgccggtggg ccacgcccac atcacgacta ccacgacgca caagacattg
      961 cggggacccc gtggcggcat gatcatgacc tccgaagaac tcggccccgc ggtcgacaag
     1021 agtgtcttcc ccggcatgca gggtggtccg ctcgagcacg tcatcgccgg caaggcggtc
     1081 gccttccgcg aggcgctcca gccgtcattc agggcctatg ccgctgccgt gatcgagaat
     1141 gcgcaggcgc tctcggacga gctggcgtcg ctcggcatgt cgatcgtctc gggcggcacc
     1201 gacacgcatc tgatgctggt cgatatcacg ccaaccggca tgagtggcaa gaaggccgag
     1261 cgcattctcg attcggttgg catcacggcc aacaagaacg ccatcccgaa cgacccgcgc
     1321 ccaccgatgc agacgtccgg catccggctc ggctcgccgg cggtcacgac gcgaggctat
     1381 ggcgtcgccg agatgcggca ggtcgcgcgg atgatcgtcg acacgctcgc caacccggag
     1441 gacgaggcgg tgctgagccg ggttcgcgac gacgtggcgg aactttcgcg ccgattcccg
     1501 cacccgggcg tcgattcgtc catctagcgc tcgcgtcaac ccatcgccat ggatcgaccg
     1561 ctgattgccg ctgccacgtt tctgacggcg ctcgcgatca gcgcgatcct cgtgcgccag
     1621 acgatcccct tcgcccgcgc gcgcggtttg ctcgatcagg ttggcggccg gcgagtcaac
     1681 ccgaaccggt tgccgcgcat cgcggggccg gccatcctga tcgcattcct ggcgggcatg
     1741 acgatgacgt ggatcctcgg cgtcgatcgc ttcgccgacg agcgcaaccg gatcctgctg
     1801 ctgattcctg cgtcgatcct gctcgtcggc gtgatgatcg tcgatgacat tcgcggcatc
     1861 cggcccctac ccaagcttct gcttcagatc ggcgtcgccc tgatcgtcgt cggcccgtat
     1921 gccttcgacc ggacgtgggg catcgccatc aaccagctca acctgccggt gcaggggacg
     1981 gtccagttcg gcccggccgt cgcgatcgtc ttcagcctct tctgggtcgt cggcatgatg
     2041 aacacgatca acctgatcga cgggctggac ggtctcgccg gttccctgac gcttgtggcc
     2101 tgcgcgatcc tctttctgca cacctatttc tggccgcgcg acgacccgca attcacgatc
     2161 tcgctgctgc cggcggcgtt gggcggggcg gtcctcggct tcctgctcta caactggcat
     2221 ccggcgcgga tcatcctcgg cgacgccggc gcgtatttcc tcgggcttgc gctggcggtc
     2281 acggcgatca tcggcggcgc caagatcgcg accacgctac tcgcgctcgg cctgcccatc
     2341 ctcgatgtcg cctgggtggc gatcagccgg gtgcaacgcg gctcgtcagt cgtgcaggcg
     2401 gatcgcgggc acctgcatca ccggttgctc gatctcggat ggagccaggc gcgaattgtc
     2461 gcttcggtcg caggcgcatc gctcgcgttc gggatcgctt cattgctctt gccgaaccgc
     2521 gagctgaagc tggccgcgat cgcgatcgtc ggcctggtgc tgatcggaac ggtcgtcatc
     2581 gtggccacgg tcgacgaaga cggcaaccgc aaactcggca cgcgattacc cggcgcccgg
     2641 agccgttgag aatcacccgg atgcaactca ttggctactg gggatcggca acatagcgcg
     2701 gggttaccac gccccgcaat cgcccgcagg gcgatgatcg ttcccgaccg gacggattcc
     2761 gaagttcgaa ccgggcctca caaggaggac cgtcgtggcc tgcggccaca gcggggcatg
     2821 gtaaccccgc gctacgtttc gggttccgat tatcgagtgg gttcggcaaa ctccgtgaag
     2881 ccctggcggg atcacggcag ggtcgctggc tactgctgca gcgcctcgaa acggacggtt
     2941 tgggtcttgc tgtcgcggtc gagcagatcg atcaggctgg tcaggagcgg agcgacatgg
     3001 ccgccgagca gccaaccggc gatgccaccg ccgacaccgc ctacgaccac gccggccagg
     3061 aagaccacaa ccgaggagtt ggattgctgt tgctcattca tccggctcgt ctccgctgat
     3121 gcctactgga tcgccaatac cgtcactgca tccgaccaaa gctcttccag ccggtagaag
     3181 tcgcgctgat cgacatcgaa gatgtgaacg acgacatcgg cgtagtcgag gacgatccat
     3241 cccgagatga attcgccctc gctgcgccgt gggaggcgcc cctcgtcgga aagatgctcc
     3301 cgcaccgcgt tcgcgactgc ccgcaattgc cgttcgttct cacccgaaca tatcacgaaa
     3361 aaatcggcaa aggaggatcg tccgctgata tccaggacga cggtgtcaga cgcgggcgtc
     3421 tcggtcagga tgtcggcaat gtgcaatgcg agcgctctgg cttcagccac gcgctcgtcg
     3481 tgtgatgtgg gatgttgatc gatttgcggc tccaaacaaa ctccgtggat gacagcgtgt
     3541 gcgactcgcc gatggtaggc ccggcaggat tcgaacctgc gaccctgggg ttatgagccc
     3601 cgtgctctgc cactgagcta caggcccaag gaccggctag aaggatacta gcacgcgtga
     3661 tcgaacgggc agaatgcacc agtgccgcta ttcgccaagt ggtggcagtc gcttgtcatc
     3721 ccaggagcgc gggtcgtcca gggattcgag gtgcgtgatc atcacgatgc cgggcacggc
     3781 ggcgcggata tcccgttcga tgcactcgac caggtggtgg ccgttgtgga ccgtccagtt
     3841 ccccggcacc aggatatgca cggaaccgaa cctgcgctga cccgcttgcc gcgttcgaag
     3901 cgcgtgcatt tctatcttgc ctggctgccg gtattgttcg agtacccgct cgatgttccc
     3961 caactcgtca ggggggaggg cggtatcgag tagcccgagc atcgaccgct tcatcaggtc
     4021 gaacccgacc cggataatgt tcgccgcgac cagcaacgcg acgatcgggt cgattcgggt
     4081 ccagccggtg aactggatca gcacgatccc gatgatcacg ccggcgctgg tcaggacatc
     4141 tgtgagcaag tgtcgcgcgc ctgcctcgag cgcgatcgag tcgtggtgct cgccggcgcg
     4201 ctggatccag cgcgcggcaa tcaaattcac gatcgacgcc gcaactgcga tcgatacacc
     4261 gagcccggct gattcgacct cggccggtga ttgcaggcgt tgcgcggcgg agtagatgat
     4321 gctgaccgcc gcgatcagga tcagcattcc ctccagcccg ctgctgaagt actccgcctt
     4381 ggcgtggccg taggcatgct cctcgtccgg ttcctgcccg gcgtaccaga gcgcgatcaa
     4441 cgccgcgatc gcgccgacga ggttgacgca tgactccagc gcgtccgaca ggagtccgac
     4501 cgagtcggtc acccaccagg cagcgaattt gagcacgatc gtgatcaccg ctgccgcgat
     4561 cgaaatccat gcgaagcggt gcagctgggt atgggccgtg tccatgtcgg aacggtacca
     4621 atcccggtcc ggtttttgcc atggacgcgc gactattccc tcttcaggca atggccagat
     4681 tgtcgatcag gcgcgtgtcc cgcgcgacga ccgcgaccag ggcgcgactg cctgaccgga
     4741 gtacgccgag cggctcaagc gtctgttcat cgacgatcgc caggtaatcg agcgtaagca
     4801 atggctgtgc gtcgatgatc atccggccac gcgcgacgag tcgaccgctg tcggtctcgc
     4861 cggcatcggc cgcatcccgc atcgtgtaca gggcttgtgg aatagctgct gctgccgcgc
     4921 gctcgtcggg agagaggcgg gcattgcggg agctgagggc cagtccatca cggtctcgca
     4981 cggtcgggcg gccgatgatc tcacccggca acagcagatc ggcgtgcatg cgccggatga
     5041 ccgccagctg ctggaaatcc ttctcgccga agtaggacct tgccggacgg acggtgttga
     5101 gcaggattgc gacgacggtc gcgacacccg cgaaatgacc gggccgggag gcgccctccc
     5161 agcggtcggt cagggcggcg accgtcacgg tcgtgctgaa cccgggcgga taaacgatct
     5221 cggcggctgg cgcatagatg atgtccgcgc ccacggtgaa ggcccgcgcg gcgtccgatt
     5281 cgagaacgcg gggataggca gcaaggtcgg tcgcgctgcc gaactgggtc ggattgacga
     5341 agacgctgac gaccgtcagt gggttatccc gggcggactg ccggatcagc gacaggtgcc
     5401 cttcgtgcag tgcgcccatc gtcggaacga ggccgaccgg ctgcccgccc atcgccgcgc
     5461 gcagctgggc aggagtagtg acgatccggc cgctggtcac tcgatgtcgt cctcatccag
     5521 cacagccagc gcggccggcg gagtcttgaa tgagtgggcg gcatcgggat aggcgcgggt
     5581 tcgcacgtca ctggcgtagg cggagaaggc ggcggtgatc gtcccgctca ggtcggagta
     5641 ggttttgagg aagcgcggcc ggaatcgctc gtccattcca agcatgtccg gcgcgaccag
     5701 cacctgcccg tcgcactcga cgcccgcgcc gatgccaatc gtcgggatgg gcgtggaagc
     5761 cgtgatccgg ccggcgagct ccgcgccgac caactcgatc acgacggcaa acgccccggc
     5821 cgcgaccact gcctgcgcat cagccatcaa ttggcgaacg gccgcctcgt ccttgccctg
     5881 gacccggtag ccgccgtcga gcccggctgt ctggggcagc aggccgatgt gggcgacgac
     5941 cgggatgcct atgcgggtga tcg
//
LOCUS       DASSGX010000023         2379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1309604, whole genome shotgun sequence.
ACCESSION   DASSGX010000023 DASSGX010000000
VERSION     DASSGX010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309604"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="VFI12_00360"
     CDS             <1..705
                     /locus_tag="VFI12_00360"
                     /inference="COORDINATES: protein motif:HMM:NF037182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HET7054875.1"
                     /translation="TQRVEAEAKRLFSGARVTRWDQDVARRRGGNAAILQAIEKREVD
                     IIIGTQLIAKGLDLPYVTTVGITNADIGLHFPDFRSGERTFQLITQMAGRAGRRTPGS
                     SVIVQTYSPDHYVLRAAANHDVEQFYEHEVRFRDEFRYPPFVRMIRYLVRGATDDDCA
                     MESDHMVRALGRHARAVGAEIEIVGPAPAFISRVRGEQQWHVIVKAAPNGIERMLNGL
                     PHPPGWAVDVDPVSLL"
     gene            762..1289
                     /gene="def"
                     /locus_tag="VFI12_00365"
     CDS             762..1289
                     /gene="def"
                     /locus_tag="VFI12_00365"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET7054876.1"
                     /translation="MAILEIIKEGDPRLRQKAHRVKNPEPGLKKLAQDMHETMDAAPG
                     VGLAGPQIGVLRRIIVVHLPEDYIEEGSPELRMTLLNPEIIKGHGEEIGLEGCLSIPG
                     WVGDVPRMTQITVKGMDLEGRSIRVKAEDFFARVLQHEIDHLDGILFVDRMAKDAKLM
                     PDREEEEDVVGAATA"
     gene            complement(1290..1946)
                     /gene="gmk"
                     /locus_tag="VFI12_00370"
     CDS             complement(1290..1946)
                     /gene="gmk"
                     /locus_tag="VFI12_00370"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HET7054877.1"
                     /translation="MVTVTDQQVLAGDQFIAELRGRRRPRVFIISGPSGVGKDSVIDR
                     LREIESDTYFAVTATTRPKRHGEVDGIHYFFLDHEQFANWVADGEFMEFATVYEQRYG
                     VPKPPVRAALQRGQDVVIKIDVQGAATIRSLIPDSIGIFIAPESMDSLYLRLSARKSD
                     DPAALARRFEEARIELLRAQEFDYIVFNETNGLLRAVDEISAIIRAVRAEIRQPEIAI
                     "
     gene            1998..>2379
                     /locus_tag="VFI12_00375"
     CDS             1998..>2379
                     /locus_tag="VFI12_00375"
                     /inference="COORDINATES: protein motif:HMM:NF017633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HET7054878.1"
                     /translation="MFDALTTAALADQLGHDIEYGRVQRLGLISRQAVWFEIYANRRR
                     TYLIASAENQAPAVYLTDDAPVSDRQLITPLLLLLRKYARGARIVAIQQPPLERVITL
                     SFSSRRTPVTDAEEQATVDVAIADD"
BASE COUNT          452 a          735 c          775 g          417 t
ORIGIN      
        1 acccagcggg tcgaagccga ggccaagcga ctcttctccg gtgcacgcgt gacccggtgg
       61 gatcaggacg tcgcccgccg gcggggcggg aacgcggcga tcctgcaggc gatcgagaag
      121 cgggaggtcg acatcatcat cggcacgcaa ttgatcgcga agggactgga cttgccctac
      181 gtgacgacgg tcgggattac gaatgcggac atcggcttgc acttccccga tttccggtcg
      241 ggcgagcgga cgttccagct catcacgcag atggccgggc gggccggacg gcgcactccg
      301 ggcagcagcg tgatcgtcca gacgtactcg ccggatcatt acgtgctacg ggcagcggcg
      361 aaccacgatg tcgagcagtt ctatgagcac gaggttcgct tccgggatga atttcggtac
      421 ccgccctttg tgcgcatgat ccgctacctg gtccggggcg cgaccgatga cgattgcgcg
      481 atggaatccg atcatatggt tcgcgcgctc ggcagacacg cgcgggcagt tggcgcggag
      541 atcgagatcg tcggtccggc gcccgccttc atctcccgtg tccgcggcga gcaacagtgg
      601 cacgtgatcg tgaaggccgc gccgaacggc atcgagcgca tgcttaacgg gttaccgcat
      661 cctcccggat gggcagtgga cgtcgatccc gtgtccctgc tttagcctgg cttcggtgca
      721 tactcagcaa tacaggacag gttgacgacg aaagcggaca aatggcgatt ctggagatca
      781 tcaaagaggg cgatccacgg ttgcggcaga aggcgcaccg ggtgaagaac ccggagccag
      841 gactgaaaaa gctcgcccag gacatgcacg agacgatgga cgcggctccc ggagtgggtc
      901 tggctgggcc gcagatcggc gtcctgcgcc ggatcatcgt cgtccactta cccgaggatt
      961 acatcgagga gggctccccc gagctgcgga tgacgctgct caaccccgag atcatcaagg
     1021 gtcacggcga ggagatcggg ctcgagggct gcctgagcat ccccggctgg gtcggcgacg
     1081 tcccgcgcat gacgcagatt acagtcaagg ggatggatct cgaaggccgc agcatccgcg
     1141 tcaaagccga agacttcttc gcgcgggtgc tccagcatga gatcgaccac ctcgacggca
     1201 tcctgttcgt cgaccggatg gccaaagacg cgaagctcat gccggaccgt gaggaggaag
     1261 aggacgtggt cggggccgcg acggcctgat cagatcgcga tctcaggctg acgaatttcg
     1321 gcgcggacgg cgcggatgat cgcgctgatc tcatccaccg cgcgcaacaa gccattcgtt
     1381 tcgttgaaga cgatgtagtc gaactcttgc gcgcgcaaca gctcgatgcg cgcctcctcg
     1441 aagcggcggg ccagcgccgc ggggtcgtcg gacttgcgcg cgctcaaccg taaatagagg
     1501 ctgtccattg attccggcgc aatgaagatg ccgattgagt cggggatcaa cgaccgaatc
     1561 gtggccgcgc cctggacgtc gatcttgatg accacatcct ggccgcgctg caacgcagca
     1621 cggacgggcg gcttcggcac gccgtagcgt tgctcgtaaa cggtggcgaa ttccatgaac
     1681 tcgccgtcgg ccacccaatt cgcgaattgc tcgtgatcca ggaagaaata atgaatcccg
     1741 tcgacttcgc cgtggcgttt aggccgtgtc gttgcggtga cggcgaagta ggtgtcggac
     1801 tcgatttcgc gcagccggtc gattacggaa tccttgccaa cgccggatgg cccggagatg
     1861 atgaagacgc gtggccggcg gcggccgcgc agctcagcga taaactgatc acctgcaagc
     1921 acttgctgat cggtgaccgt caccattctg ttcacctggc gcaatgacga tcgattcact
     1981 gggacgatgt tgacctcatg tttgatgcgc ttaccacggc ggccctggcg gaccagctcg
     2041 gccacgatat cgagtacggc cgtgttcagc ggctcggatt gatctcccgc caggctgtct
     2101 ggttcgagat ctacgccaat cgccgccgca cgtacctgat cgcctccgcc gagaaccagg
     2161 cgcctgcagt ctacctgacc gacgacgcgc cggtgtcgga tcggcagctc atcacgccgc
     2221 tcctcctgct cctgcgcaag tatgcccggg gcgcgcgaat cgtcgcgatt cagcagccgc
     2281 cactggagcg ggtgatcacg ctgtcctttt cgtcgcgccg caccccggta accgatgcgg
     2341 aagagcaggc gaccgtcgac gtcgctattg ccgacgacg
//
LOCUS       DASSGX010000024         3756 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_438822, whole genome shotgun sequence.
ACCESSION   DASSGX010000024 DASSGX010000000
VERSION     DASSGX010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438822"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..1552
                     /locus_tag="VFI12_00380"
     CDS             107..1552
                     /locus_tag="VFI12_00380"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HET7054879.1"
                     /translation="MRQSREVMLGVDSSTQSTKVVAVDLETGEIVSEGRAPHTGADTQ
                     DPTDWWSALATAVNVAVTDEMEVRGIAVAGQQHGFVTLDADGNPVRPAPLWNNVASAP
                     DAARLNDAVDFAEAVGSRLVASFTITKLAHLAHTAPAELDRIAAICLPHDYLNLRLTG
                     NLATDRSEASGSGWWSSDTGVRRDLLALTAGPAFAERVRVPNVLGPQAIAGTLTASAA
                     AALGLPAGIPVGPGAGDNAGAALGIGATTAELCISLGTSGVATAVSDSPTNDATGEVA
                     GFADASGKYLPLTCMLNCTRVVGTIADMFGKSVVEALDIAGMLEPGADRLLLLPYLSG
                     ERTPDLPYATGIASGLTATNLRAETWIRAAVDGVAAGLAYCQEALARVDVCKEIVTLV
                     GGGSRHATWQQAIADATGLPVQVRGGGEHVARGAAVQIAAIVRDEPVAHLAEAWRPDV
                     IAHATPRAELRGAFRLSERRELIAAMRAGSA"
     gene            complement(1553..3058)
                     /gene="xylB"
                     /locus_tag="VFI12_00385"
     CDS             complement(1553..3058)
                     /gene="xylB"
                     /locus_tag="VFI12_00385"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HET7054880.1"
                     /translation="MSYLGIDLGTTSLKAVLVSVSGEVLREGTASYPMQHPYPGAAVQ
                     DPEHWWRAARDAVQQIQNGLGADINTIGLSGQMHGTVCLNSMRQLIRPALIWPDTRGT
                     VTAAELTAVVGEMRLADAIGTPLASGFQGVTVAWLRQHEPTTFRSIATVLLPKDYLRY
                     RLTGELATDPSDAAGTGMLDVHTRDWSDLMLDTVGLDRGKVPAIRPSGQIAGRLTSEA
                     AGQLGLPVGTPVVTGGGDAPLAALASGVADGRSLLATLSSGAQVIAFTDFPAVDSRLR
                     THTFAAPLDPTQGENGWYVMGATMVAGMALTWLKENIFQSNSDQAIQSMVQRASEVSP
                     GSNGLLFAPYLTGERTPHLDSRARAAFLGLTADHDRRHLTRAVMEGVVFALRDALDVV
                     RSLSRTGHDVVLAGGGSRSPLWRQIMADIFGMPVRPSLVVDQSAVGAALLAASCDMGT
                     PASQLGKEWARFDRSVEPVPANMARYEELRGIFRDIYPAHAAHFHRLAEFD"
     gene            complement(3055..>3756)
                     /locus_tag="VFI12_00390"
     CDS             complement(3055..>3756)
                     /locus_tag="VFI12_00390"
                     /inference="COORDINATES: protein motif:HMM:NF014801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HET7054881.1"
                     /translation="GQGFTQGAHVVPDQYYGLGAIYTAGASYEWARKAVGGGRSHDEL
                     IAGARSVPAGSLGVMFLPHLRLAMFPKLDSRARGAFIGLTTDVTSDALIRSVLEGVAM
                     EGRAGFGPLARYARLPELTDIKVIGGTSRNQLLIEIKASVMNARHHVLEIEEATALGA
                     AMLAGMAAGVYRSADDAIACVRSAARIVEPDADVVALYDTLFEDIYRHLYGALQPMHH
                     RLFDLFISQSEDGSA"
BASE COUNT          650 a         1326 c         1224 g          556 t
ORIGIN      
        1 atcctggata tcgccccgcc attatcgatg attggcggat gcgcgggctg cctcgctccg
       61 gcaaaatagc ggttgaaatc gcagcggtat caggcggggc tcacgaatgc gtcaatcacg
      121 ggaagtcatg ctcggtgtcg actcgagcac gcagtcgacg aaggtcgtcg cggtcgatct
      181 cgaaactgga gagatcgtct cagagggccg cgctccgcac accggcgcgg atacgcagga
      241 cccaacagac tggtggtcgg ctctcgccac ggcggtcaat gtcgcggtga ccgacgagat
      301 ggaagtgcgc ggcattgcgg tcgccggcca gcagcacggc ttcgtcacgc tcgacgccga
      361 cgggaatccg gtccggccgg cgccgctctg gaacaacgtt gcctcggcgc cggacgccgc
      421 gcggctgaat gacgcggtgg acttcgcgga agcagtgggc tcccgcctcg tcgcatcctt
      481 cacgatcacg aaactggcgc acctggctca caccgccccg gctgagctcg atcgcatcgc
      541 ggccatctgc ctgccgcacg actatctgaa tttgcgactc accggaaatc tcgcgacaga
      601 tcggagcgag gcgtcgggca gcggctggtg gtcatccgac accggcgtcc gccgtgatct
      661 tcttgcactt accgccggcc ccgcattcgc cgagcgggtc cgcgtgccca acgtgctcgg
      721 cccgcaggca atcgccggga cactgaccgc ttcggccgcg gcggcgctcg ggttaccagc
      781 cgggatcccg gtcggaccgg gcgcggggga caatgccggc gcggccctcg ggatcggcgc
      841 gacgacagcc gagctctgca tcagcctcgg caccagcggc gtcgccaccg ccgtctccga
      901 ctcaccgacg aacgatgcaa ccggcgaggt cgcgggcttt gccgacgcgt ccggcaaata
      961 cctgccgctg acctgcatgc tgaactgcac gcgcgtcgtc ggcacgatcg ccgacatgtt
     1021 cggaaaatcc gtggtcgagg cgctggacat cgccggcatg ctcgagcccg gcgccgatcg
     1081 attgctgctg ctcccctacc tgagcggaga acgcacgccg gatctcccct acgcgactgg
     1141 catcgcctcc gggctcacgg ccaccaacct gcgagccgaa acatggatca gggcggcggt
     1201 cgacggcgtc gccgccggcc ttgcctattg tcaggaggcg cttgcccggg tcgatgtctg
     1261 caaggagatc gtgacgttgg tcggcggcgg ctcccgccac gcgacctggc agcaggccat
     1321 cgccgatgcg accggactac cggtccaggt ccggggcggc ggcgagcatg ttgcccgggg
     1381 cgcggcggtc cagatcgccg ccatcgtccg ggacgagcca gtcgcccatc tagccgaagc
     1441 gtggcggccc gacgtgatcg cccacgccac gccccgagcc gagcttcgcg gggccttccg
     1501 gctcagcgag cgacgcgagc tgatcgcggc gatgagggcc ggctcggcct gatcagtcga
     1561 attcggccag gcggtggaaa tgggccgcgt gggccgggta gatgtcccgg aagatgccgc
     1621 gcaattcctc gtaccgggcc atgttggccg gaaccggctc gaccgaccgg tcgaatcgcg
     1681 cccactcctt gccgagctgg ctcgccggcg tgcccatatc gcatgacgcc gccaataatg
     1741 ccgcgccaac cgccgactgg tcgacgacca gggagggccg caccggcatc ccgaagatat
     1801 cggccatgat ctgccgccag agcgggctgc gcgagccgcc accggccagc acgacgtcgt
     1861 gcccggtgcg cgacagtgat cgcacgacat cgagcgcgtc gcgcaacgcg aagaccacac
     1921 cctccatgac cgcccgggtg aggtgccggc ggtcatgatc cgccgtcagc cccagaaatg
     1981 ccgcccgcgc ccgcgaatcg aggtgcggtg tgcgctctcc cgtcaggtac ggcgcaaaga
     2041 gcagaccatt cgaccccggc gagacctccg atgcacgctg caccatggac tggatcgcct
     2101 gatcggagtt cgactggaag atgttctcct tcagccaggt cagcgccatt cccgccacca
     2161 tcgtcgcgcc catcacgtac cagccgttct cgccctgggt cggatcgagc ggcgcggcaa
     2221 aggtatgcgt gcgcagccgg gaatcgaccg cggggaagtc cgtgaacgca atcacctgcg
     2281 cgcctgagct gagcgtcgcc agcagcgacc ggccatcggc aacaccggaa gcaagcgcag
     2341 caagcggagc atcgcccccg ccggtgacga ccggggtgcc gaccggcaac ccgagctgcc
     2401 ccgccgcctc ggacgtgagc cggccggcga tctggccaga cgggcgaatg gccgggacct
     2461 tgccgcgatc cagcccgacg gtatcgagca tcagatcaga ccagtcccgt gtgtgcacgt
     2521 ccagcatgcc cgttccggcc gcatcgctcg ggtcggtggc caactcgccc gtcagccgat
     2581 accgcaggta gtccttgggc aagagcaccg tcgcgatcga ccggaaggtt gtcggctcat
     2641 gctgccggag ccaggcgacc gtgacaccct gaaacccgga ggcgagcggc gtgccgatcg
     2701 cgtccgctag tcgcatctcg ccgaccaccg cggtcagctc agccgccgtg accgtccccc
     2761 gcgtatctgg ccagatcagc gccggccgga tgagctgccg catcgagttg aggcagaccg
     2821 tgccatgcat ctgccccgac agcccgatcg tattgatgtc ggcgcccaaa ccgttctgaa
     2881 tttgctgcac ggcgtcgcgg gccgcccgcc accaatgctc tgggtcctgc accgcggcgc
     2941 ccggatacgg gtgttgcatc ggatagctgg ccgtgccttc gcgcagcacc tcgccagaca
     3001 cgctgacgag gacggccttg agcgacgtcg tgccgagatc gatacccagg tagctcatgc
     3061 cgatccatcc tcgctttgtg agatgaacaa gtcgaacaaa cgatgatgca ttggctgcaa
     3121 cgctccgtag aggtgccggt aaatgtcctc gaacaaggtg tcgtagagcg caacgacgtc
     3181 cgcgtcgggc tcgacaatgc gggccgccga ccgaacacaa gcgattgcgt catcggccga
     3241 acgataaacg cccgcggcca taccggccaa catggcggcg ccaagcgccg tcgcctcctc
     3301 gatctccagc acatggtggc gggcattcat gaccgaggcc ttgatctcga tcaggagctg
     3361 gttgcgcgat gtgccgccga tcaccttgat atcggtcagc tccggcagcc gcgcataccg
     3421 ggcgagcgga ccgaacccgg cccgtccctc catggccacg ccttcgagca ccgagcggat
     3481 gagcgcatcg gaggtaacat cggtcgtcag cccgataaag gcgccgcgcg cccgcgaatc
     3541 gagcttcggg aacattgcca aacggaggtg cggcaagaac atgaccccga gcgagccggc
     3601 cggtaccgac cgcgccccgg cgatcaattc atcgtggctc cggccgccac cgaccgcctt
     3661 gcgcgcccac tcgtagctcg caccggcggt atagatcgca ccgagcccgt agtactgatc
     3721 cggcacgacg tgcgcgccct gcgtgaagcc ctggcc
//
LOCUS       DASSGX010000025         5404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1309909, whole genome shotgun sequence.
ACCESSION   DASSGX010000025 DASSGX010000000
VERSION     DASSGX010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5404
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1309909"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="VFI12_00395"
     CDS             <1..669
                     /locus_tag="VFI12_00395"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET7054882.1"
                     /translation="PIWPILCRLRSIAELAAAEIHRVSEQNQQSVDVVIETPIGPITV
                     RLDTGKAPVTASNFLSYADRGFYDGGRFHRTVRLDNQSNAGLKIDVTDLGIADTGGTK
                     PLPNDQIPIEVIQGGINPARKAEQGPPILLERTSVTGLSHRDGTISMGRTTADTAVSD
                     FFICINDQPELDFGGRRNVDGQGFAAFGQVIAGMDFVRAIQTAPSEGQSLTPPVPITR
                     VSRA"
     gene            complement(682..1551)
                     /locus_tag="VFI12_00400"
     CDS             complement(682..1551)
                     /locus_tag="VFI12_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7054883.1"
                     /translation="MTPWNAKDIDGGRPIGFDPGKTGRVLLWTAIVTFCGAAFASIIV
                     ILSGGFGDTEAKILLSSFSIAAYSLAGLIATARFGRRPAFLAPLGLGSAALGLVLTLA
                     LIWTEADSEALWRITLSVMVITGAIAHANLLLGNQERRDPAALILKGTLAVNALLTAL
                     IVIPILAGSEPDGGYWKLLAVLAILLVLGTLVVPIVRKMSDAGDRPTDTAPPVASSDG
                     AGQLEMRYRGRQFRIEVDTDGGSAPAVRAWAVGGAEERPILLNQDTTPADAHAALANA
                     VQQITRAVDSGMS"
     gene            complement(1672..2652)
                     /locus_tag="VFI12_00405"
     CDS             complement(1672..2652)
                     /locus_tag="VFI12_00405"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HET7054884.1"
                     /translation="MKPFAYTSAESVAEAIAILAQEGGNGTRPLAGGTDLLTLMKSEI
                     ETPTHLVDIKRASDLSDAIEATDDGVMLGALTTLAQIERHEVLRERFPAVAQAAGLAA
                     TPQLRNMATIAGNLLQRPRCWYYRSDKVECWLKGGADCPARDGENQLHALFPADPCVA
                     VHPSDLASALLAFDAGLTVRGPGGERRIALDDFFAMPENDRRTENLLRADELIIAVHL
                     PAPDSVVRSVYLKAMDRNVWAFALVGVALAARLEGTRIAAARLVLSGVAPIPWRVPAA
                     EELLVGRDLGSIDAEQMADAALADAQPLRLNGYKVPLAKALIKRALAEIG"
     gene            complement(2649..4955)
                     /locus_tag="VFI12_00410"
     CDS             complement(2649..4955)
                     /locus_tag="VFI12_00410"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET7054885.1"
                     /translation="MSGFVVEKTGGGSGADGQYQVKQPTPVEPWGAGAELRYVGQRHT
                     RVEGVEKVTGSARFAYDVQLPGQLVGRVVRSPHPHARVTRIDATKAEALPGVHAVVSS
                     ANTREIPWYADSFVFDPTVRFIGEEVAAIAAETEEIAGDALRLIEVEYELLPFVTDLE
                     TALAPDAPKVRESGNIADEPTIYERGDVESGFREAEVTVDQIYTTQCALHNSFEPHGC
                     TASWEGDNLTLWDSTQSIFTVRDQVAKALDMPSHRVRVIKQFMGGGFGAKQIAWKQDV
                     IASLLSKASGRPVQLMLDREAENLAVGNRNPTHQRVRLGSKRDGTLTAISVEIHQQAG
                     AHMTGGEASDVSAMYQTLYRCPNVRTDQTIVYTNTGPAVAFRAPGHVESAAALECAMD
                     ELARALEMDPSELRLRNYSERDQQKDQPYTSPESLSRCSEQATRAFGWQDYRKPEPDG
                     PKRRGIGFAAHDWGGGGQPPGYAWVKLNPDGTADVVTGTQDIGTGTRTGLAQVAAEEL
                     GMPIEDVRLHLGDTAYGPYAPVSSGSATQATIGPAIRAAAFDAKRQLFAAAAVYLETQ
                     PVALSIHDGKLFVAGRSGQDGVPVGDVMSAIAPVMVLGQGAREPNPEGKTIRTVGAQC
                     IEVEVDIETGDVQVLRVVASHDCGRIVNPTMVDSQIIGAVTQGIGFALTEERVVDPRL
                     GIVLNANLEEYKVPTVADIPLIEHAAVDVPDVEANSTGAKGIGEPPLVPTAPAIANAV
                     FDAIGIRFREFPLSRRRVLDALAGGEAR"
     gene            complement(4952..>5404)
                     /locus_tag="VFI12_00415"
     CDS             complement(4952..>5404)
                     /locus_tag="VFI12_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET7054886.1"
                     /translation="GVEQAISVDARRTLLDTLRSDLRLTGTKKVCNMGDCGACTVLMD
                     GCAVYSCLVLAADLAGREITTIEGLDRHGELDPVQRAFIADDAFQCGFCTPGQIMSLR
                     ALLDENPDPTDEEIERAVTGNLCRCGAYRNILAAGRQAAELQREGATP"
BASE COUNT          856 a         1987 c         1661 g          900 t
ORIGIN      
        1 ccgatctggc cgatactgtg ccggttgcgg agcatcgcag aactagcagc agcggagatt
       61 caccgggtga gtgagcagaa ccagcaatct gtcgatgtcg tcattgagac gccgataggt
      121 cccattaccg ttcgattgga tacggggaaa gcaccggtca cggccagtaa cttcctctcc
      181 tacgccgatc gcggcttcta tgacggcggt cgctttcacc gcacggtccg tctcgacaac
      241 cagtcgaacg ccggcttgaa gatcgatgtc accgacctcg gcattgccga taccggcgga
      301 acgaagccgc ttccgaacga ccagatcccg atcgaggtca tccagggcgg catcaacccg
      361 gcccgcaagg ccgagcaagg cccgccgatc ctgctcgagc gaaccagcgt gaccggttta
      421 tcccatcgcg acggcacgat ctcgatgggc cggaccaccg ccgataccgc cgtctccgac
      481 ttcttcatct gcatcaacga ccagcccgag ctcgacttcg gcgggcgccg caacgtcgac
      541 ggtcagggct tcgccgcctt cggccaggtc atcgccggca tggactttgt ccgcgccatc
      601 caaaccgccc ccagcgaagg ccaatccctc actccgcccg tcccaatcac ccgggtcagt
      661 cgggcgtaat caacagggca ttcacgacat tccggaatca actgcccggg tgatttgctg
      721 gacggcgttg gccagggccg cgtgcgcgtc ggccggagtc gtgtcctggt tgagcaggat
      781 cgggcgctcc tcggccccgc cgaccgccca cgcccggacc gcgggcgcgg acccgccgtc
      841 ggtgtcgact tcgatccgga actggcggcc ccggtagcgc atctcgagtt ggccggcgcc
      901 atcgctcgac gcgaccggtg gagcagtgtc cgtcggcctg tcgccggcat cgctcatctt
      961 ccgcacgatg ggtacgacca acgtgccgag caccagcaag atcgccagca ccgcgagaag
     1021 cttccagtac ccgccatccg gctcgctgcc ggccaggatc gggatcacga tcagcgcggt
     1081 cagcagcgcg ttgaccgcca gcgtcccctt caggatcagc gcggccggat ccctccgctc
     1141 ctgattgccg agcaggagat tcgcgtgggc gatcgccccg gtgatgacca tgacgctgag
     1201 cgtgatccgc cagagcgcct cgctgtcggc ctcggtccag atcagggcca gcgtcagcac
     1261 cagcccaagg gccgcgctgc cgagcccgag cggagccagg aacgccggtc tccgcccaaa
     1321 tcgcgcggtc gcgatcaggc cggccaggct gtaagccgcg atcgagaacg aactgagcag
     1381 gatcttggcc tcggtatcac cgaacccacc cgagaggatg acgataatgg aagcgaacgc
     1441 cgctccacaa aaggtgacga tcgcggtcca gagcagaacc cggccagtct tgcccgggtc
     1501 gaacccgatc ggccgtccac cgtcgatgtc cttcgcattc cagggtgtca tgttgggcgc
     1561 tcatcccgca gtaagccgac gttctgcgcc caatctattc cagtgcaggc aattccggaa
     1621 tgcccttcgg gtcatgcagc gcgaaagccg ccgctcacag cctctgacac gtcatccaat
     1681 ttcagccagc gctcgcttga tcaatgcctt cgccagcggg accttgtacc cattcaatcg
     1741 gagcggctgg gcgtcagcga gcgcggcgtc ggccatctgc tcggcgtcaa tgctcccgag
     1801 atcccggccg accagaagtt cctcggccgc cggaacgcgc cacgggatcg gcgccactcc
     1861 gcttagcacc agtcgcgcgg cggcgatccg cgtcccctcg agccgcgccg cgagcgccac
     1921 cccgaccagg gcgaaggccc agacgttgcg gtccatcgcc ttcaggtaga cgctccgcac
     1981 gaccgagtca ggcgccggca aatggacggc gataatcaac tcatccgccc gcaggagatt
     2041 ctcggtgcgc cggtcattct ctggcattgc gaagaaatcg tcgagcgcta ttcgccgctc
     2101 cccacccggc ccgcgcacgg tcagcccggc atcgaacgcg agcagggccg acgccaggtc
     2161 cgacggatgc accgccacgc acggatcagc cgggaaaagc gcatggagct ggttctcgcc
     2221 gtcgcgcgcc gggcaatcag ccccgccctt gagccagcac tcgaccttgt cgctccggta
     2281 ataccagcag cgtggccgct ggagcagatt cccggcgatc gtggccatgt tccggagttg
     2341 gggcgtcgcc gccaacccag ccgcctgcgc gacggccggg aaccgttcgc gtagcacctc
     2401 gtgccgctcg atctgcgcca gcgtcgtcag cgctccgagc atcacgccgt cgtccgtcgc
     2461 ctcgatcgcg tcggagagat ccgatgcccg cttgatgtcg accaggtgcg tcggcgtctc
     2521 gatctccgac ttcatcagcg tcagcagatc ggtgccgccg gccagcggtc gcgtcccgtt
     2581 cccaccctcc tgggcgagga tggcgatcgc ttcggccacg ctctcagcgc tcgtgtaggc
     2641 aaacggcttc atcgcgcctc ccctcccgcg agcgcatcca gtacccgccg gcgcgagagc
     2701 ggaaactccc ggaaccggat cccgatcgca tcgaagacgg cattcgcgat cgcgggcgcg
     2761 gttgggacca gcggcggctc gccgatcccc ttcgccccgg tcgagttcgc ctcgacgtcc
     2821 gggacatcga ccgccgcatg ctcgatcagc gggatatcgg ccacggtcgg caccttgtac
     2881 tcctcgagat tggcgttgag gacgattccg aggcgcggat cgacgacccg ctcctccgtc
     2941 agcgcgaagc cgatcccctg cgtcacggcg ccgatgatct ggctgtcgac catcgtcggg
     3001 ttcacgatcc ttccgcagtc gtgcgacgcc accacccgca gcacctggac atcgccggtc
     3061 tcgatatcga cctcgacctc gatgcattgc gcgccgacgg tgcgaatcgt tttaccctcc
     3121 gggttcggct cgcgcgcgcc ctgccccagc accatcaccg gcgcgatcgc cgacatcacg
     3181 tcgccgaccg gcaccccatc ctgtccggac cttcctgcta cgaagagctt gccgtcgtgg
     3241 atgctgagcg cgaccggttg cgtctcaagg tagaccgcgg ccgccgcaaa gagttgccgc
     3301 tttgcgtcga acgccgccgc ccggatcgcg ggaccgattg tcgcctgcgt cgcgctgccg
     3361 gagctgaccg gcgcatacgg cccgtatgcc gtgtcaccga ggtggaggcg cacatcctcg
     3421 atcggcatcc ccagttcctc ggccgcgacc tgcgccaggc cggtccgcgt gccagtgcca
     3481 atgtcctgcg tgccggtcac gacgtcggcc gtgccatccg ggttcagctt gacccacgcg
     3541 tagcccggcg gttgcccgcc gccgccccag tcgtgcgccg cgaagccgat cccacgccgc
     3601 ttcggcccat ccggctccgg tttgcgatag tcctgccagc cgaacgcccg cgtcgcctgt
     3661 tcggagcaac ggctcaggct ctccggcgag gtgtatggct gatccttctg ctggtcgcgc
     3721 tcggaatagt tgcgcagccg cagttcagag gggtccatct ccagcgcccg ggccagctcg
     3781 tccatagcgc actcgagcgc ggcggccgac tcgacgtgcc ccggcgcccg gaatgcgacc
     3841 gccgggcccg tgttggtgta gacgatcgtc tggtccgtac gcacgttcgg gcagcgatag
     3901 agcgtctggt acatcgcgct cacgtcgctc gcctcaccgc cggtcatgtg ggcgccggcc
     3961 tgctgatgga tctcgaccga gatcgccgtc agtgtgccgt cccgcttcga cccgagccgg
     4021 actcgctgat gcgtcggatt gcgattgccg actgccaggt tctcggcctc gcggtcgagc
     4081 atcaactgaa ccggccggcc cgacgctttc gagaggagcg acgcgatcac gtcctgcttc
     4141 caggcaatct gcttggcccc gaagccgccg cccatgaact gcttgatcac gcgaacgcga
     4201 tgggacggca tgtcgagcgc cttcgcgacc tgatcccgca ctgtgaagat cgactgcgtc
     4261 gagtcccaga gcgtcaggtt gtctccctcc cagctcgccg tgcaaccgtg cggctcaaaa
     4321 ctgttgtgaa gcgcgcactg cgtcgtgtag atctggtcga ctgtgacttc tgcctcgcgg
     4381 aagccgctct ccacatcgcc ccgctcatag atggtcggtt catcagcgat gttgccgctc
     4441 tcccgcacct tgggcgcatc cggcgccagc gccgtctcca aatcggtcac gaacggcagc
     4501 agctcgtact cgacctcaat cagccgcagg gcatccccgg cgatctcctc ggtctccgcc
     4561 gcgatcgccg cgacctcctc gccgatgaac cgcaccgtcg ggtcgaagac gaagctgtcg
     4621 gcgtaccacg ggatctcccg cgtgttcgcc gagctgacca cggcgtggac cccgggcagc
     4681 gcttccgcct tcgtcgcatc gatccgcgtg acccgggcgt gcgggtgcgg gctgcgcacg
     4741 acccggccga ccagttgccc cggcagctgc acgtcgtagg cgaaccgggc gctgccggtc
     4801 accttctcga cgccctcgac ccgcgtgtgc cgctgcccga cgtatcgcag ctctgccccg
     4861 gcgccccacg gctcgaccgg tgtcggctgc ttgacctgat attgcccgtc cgcacccgat
     4921 ccgccgccgg tcttctcgac gacgaagccg ctcatggcgt cgctccttcc cgctggagct
     4981 cggccgcctg ccgcccggcc gcgaggatgt tccggtacgc cccgcagcgg cagaggttcc
     5041 cggtcaccgc ccgctcgatc tcctcgtcgg tcgggtccgg attctcgtcg agcagcgcgc
     5101 gcaggctcat gatctggccc ggcgtgcaga atccacactg gaacgcatcg tcggcgatga
     5161 aggcccgctg cacaggatcc agttcgccat gccggtcgag cccctcgatc gtcgtgattt
     5221 cccgcccggc caggtccgcc gccaggacga ggcaggagta cacagcgcac ccatccatca
     5281 ggacggtgca ggcgccgcaa tcgcccatat tgcagacctt cttcgtgccg gtcaggcgca
     5341 ggtcggagcg cagcgtgtcg agcagcgtcc gccgcgcgtc gactgagatc gcctgctcga
     5401 cccc
//
LOCUS       DASSGX010000026         2778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_983530, whole genome shotgun sequence.
ACCESSION   DASSGX010000026 DASSGX010000000
VERSION     DASSGX010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983530"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VFI12_00420"
     CDS             complement(<1..546)
                     /locus_tag="VFI12_00420"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HET7054887.1"
                     /translation="MTKLPHRVLWRLAALFILVGACAMRPGGAMAHARLTDSSPVSGS
                     ALADMPGEIVLTFSEPVVPSSVNLALTRADGARIPLSGMQPAGDDHRVSIGIDQAAPG
                     QGTFQLAWSVRSASDGHDSAGIIAFTVGTGRAPVGISSTGAERDPWWQIGLRAVWLLA
                     LALVAAGWIGSVALRERLNRAI"
     gene            545..1294
                     /locus_tag="VFI12_00425"
     CDS             545..1294
                     /locus_tag="VFI12_00425"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HET7054888.1"
                     /translation="MVVSYCGAQAGPRVVHPLRRTSQIRMPPGHDENRDDDVEARRWW
                     SRLRPNPTHPVATDGHPEVEPGEVIREGERVRLRRHTVENRPAFQRWYADDDIARLLR
                     HDLRPLTPVQSLVYFDTVILPSSARGLSLAIHDRETDELIGTTGLTDVDQRISKSCYF
                     RILIGESRYWNHGYGTEATRMMMSEAFERHGLESVKLEVFDYNHRAIAAYERVGFHMV
                     GEHTEWPEASGGSLHVLEMRLDKADFLAEAL"
     gene            1471..2292
                     /locus_tag="VFI12_00430"
     CDS             1471..2292
                     /locus_tag="VFI12_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HET7054889.1"
                     /translation="MGKTINRLRAERDVPTHDSVSVDIVIPVYNEERALPVSIPTLCR
                     YLEMYVPYQWSVTIADNASIDRTLEVANELAAADSRVNVLHLDQKGRGRALKAAWLAS
                     EADIVSYMDVDLSTNLSSFMPLIAPLATGHSEVAIGSRLSKGAVVTRQWKREVISRTY
                     NLMIKLAFRNQFSDAQCGFKALTREAAQALLPKVENNQWFFDTELLLLAEERDLRIHE
                     VPVDWVEDLDTRVKIVSTVMEDVKGLWRVRRQRLQRRHASLARKIGIENTALSAD"
BASE COUNT          506 a          855 c          920 g          497 t
ORIGIN      
        1 tattgcccgg ttaagccgtt cgcggagcgc aacggagccg atccagccgg ctgcgacaag
       61 cgccagggcg agcagccaga cagctcgcag gccaatttgc caccagggat cgcgctcggc
      121 tccggtcgag cttatcccga cgggtgctcg cccggtcccg accgtgaagg cgatgatgcc
      181 ggcggaatcg tgaccatcgc tcgcggaccg caccgaccag gcgagctgga acgtgccctg
      241 gcctggcgcc gcctgatcaa tgccgatcga aacgcggtgg tcgtccccgg cgggctgcat
      301 cccggacagc ggaatgcggg cgccatccgc tcgcgtgagg gcgaggttca ccgaggacgg
      361 gacgaccggt tccgaaaagg tcaggacaat ctcgccgggc atatccgcca gcgcgctgcc
      421 gctgacgggc gacgagtcgg tcagccgggc gtgcgccatt gcgcctcccg gccgcatcgc
      481 gcaggcgccg acgaggatga agagcgccgc aagacgccac aaaacccggt gaggcaactt
      541 ggtcatggtg gtatcgtact gtggcgcgca ggccggtccg cgcgtcgttc acccgctccg
      601 ccgcacgagc cagatccgca tgccgccagg acacgatgaa aaccgggacg atgacgtcga
      661 agcgcgacgc tggtggagtc gcctccgccc gaatccgacc cacccggtcg cgaccgacgg
      721 ccacccggaa gtcgaacccg gcgaggtcat ccgcgagggg gagcgagtcc ggctccgcag
      781 acacacggtc gagaaccggc cggcgttcca gcgctggtat gccgacgacg acattgcgcg
      841 gctgctccgc catgatctcc gcccgctgac gccggtccag tcgctcgtct acttcgatac
      901 cgtcatcttg ccgagctccg cgcgcggcct ctcgcttgcg atccacgacc gggagacgga
      961 cgagctgatc gggaccacgg ggttgaccga cgtcgatcag cgcatctcga agagctgtta
     1021 cttccggatc ctgatcggcg agtcgagata ctggaatcat gggtacggga ccgaggcgac
     1081 gcggatgatg atgtccgagg cgttcgagcg gcacgggctg gaatcagtca agctcgaggt
     1141 cttcgactat aaccaccggg ccatcgccgc atacgagcgc gtgggcttcc acatggtcgg
     1201 cgagcatacc gaatggcccg aggcgagcgg gggaagcctg cacgtgctcg agatgcgcct
     1261 cgataaggcg gacttcctgg ccgaggcgct ctgagtatcg ggactcttcg gctagtccgc
     1321 aggaatcgca cttccggtgt ttggcgtaga caatagtggc gcgcgattgc gggtattctt
     1381 gtacctgcgg tgcaagtccg ccgaacacgg ggggttgaga agctcccggt cgtaccaggc
     1441 tatgtctcct ggcactggag ccgcgaagag atggggaaaa ctatcaatcg tttgcgcgcg
     1501 gagcgcgatg tgccgacgca cgactcggtc agcgtcgaca tcgtgattcc ggtgtacaac
     1561 gaagaacgcg ccctgcccgt ctcgattccc accctgtgtc gctacctcga gatgtacgtg
     1621 ccgtaccagt ggtcggtcac gattgccgac aatgcatcga tcgaccgcac gctggaggta
     1681 gccaacgagc tggccgccgc cgattcccgg gtcaacgtgc tccaccttga ccaaaagggc
     1741 cggggccgcg cgctcaaggc ggcctggctg gcatccgaag ccgacatcgt ctcatacatg
     1801 gacgtcgacc tctcgaccaa cctcagctcc ttcatgccgc tgatcgcgcc gctggcgacc
     1861 ggtcattccg aggtagcgat cggctctcgc ctgagcaagg gcgccgtcgt cacccggcag
     1921 tggaagcgcg aggtgatctc gcggacgtac aacctgatga tcaagctggc gttccggaac
     1981 cagttctcgg acgcgcagtg cgggttcaag gcgctgaccc gggaagccgc gcaggcgctg
     2041 ctgcccaagg tcgagaataa ccagtggttc ttcgacaccg agctgcttct gctggcggag
     2101 gagcgcgacc tgcggattca cgaggtgccg gtggactggg tcgaggatct tgacacgcgg
     2161 gtcaagatcg tctcgaccgt gatggaggac gtcaaggggc tctggcgcgt gcgccggcaa
     2221 cggttgcagc gcagacatgc gtcgctggcc cgcaaaatcg ggatcgagaa caccgcgctc
     2281 tctgcagatt aggtgagatt ttgttgtctg gttgcttgac gctccgacac aaatccgtct
     2341 aggattcgaa cgtcgtattg tcccgctgga tgctgactgg aacggagtct gaacactcaa
     2401 tcaggtccgt tgcaaacggt gggtgcccgc accatcgcat ggctgaattg ttgcgtcggt
     2461 gtgtggaata tgaggagggg ttgtgtctga aaatgagcgt cggctcgcgg atcggctgga
     2521 tacccgggac ctgttgctac gtggattcag tgctattaca ttgattctgg ttgcattcct
     2581 cgctctgacc gttctgtaga catcgacgtc cagaataaat cctcggcccg catcatcgag
     2641 atgtgggccg ttttgctgcc cggcgagtcg tcattggcgg tcatcgcgcc atggtcgggg
     2701 caggctcgcg atgatggccg ggtcgacccg tagctggagc agatgttgct tgccgcacat
     2761 cggacagttg gttgcgat
//
LOCUS       DASSGX010000027         2003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_983549, whole genome shotgun sequence.
ACCESSION   DASSGX010000027 DASSGX010000000
VERSION     DASSGX010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983549"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..1535
                     /locus_tag="VFI12_00435"
     CDS             117..1535
                     /locus_tag="VFI12_00435"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET7054890.1"
                     /translation="MTAPCDLSMIEARQRMQDGSLTAVALMESVLDRMPAIPTEYHPY
                     VVIRDRESLMADAAAADHARASGESTGLLHGIPIGIKDIVDVCGFPTKCGSRSMEDCA
                     PAIEDAPLVAGFKAAGGIILGKTVTQEFAAGTISAPSRNPWDLDRIPGGSSGGSAAAI
                     AAGMALMTIGTDTGGSIRCPASVCGVTGLKPTYGTVSRRGIYPLAWSLDTAGPIARTV
                     EDCAIALDTIAGHDPRDPGSAPVRHGSAAAEIGQDIRGLRIGLPRAFFYDKLEPELSD
                     IFDQAAQCLRELGADVIEVDWDLALEARLVSMVINRAETSAVHDDRVRTHPDLVGDEW
                     RLRVKAGLLLPARNYITAHQARIVVRQSVANYFNQNRLDAMIAPATAGTAARADDPFL
                     TYTDGSREHVLSGYTRMTMPTNATGQPAISVPAGFTSAGLPIGMQIIGRPFAEARICR
                     IGHAYEQATRWIDRRPPLTQGA"
     gene            1537..1752
                     /locus_tag="VFI12_00440"
     CDS             1537..1752
                     /locus_tag="VFI12_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7054891.1"
                     /translation="MDQQDFGSVARRGAQARANLVSALRECSELADAVETFHDDELLE
                     VLAHLDSLRFVMAESGQILAGVIRGFD"
BASE COUNT          350 a          658 c          663 g          332 t
ORIGIN      
        1 tcggtgatgg agcggcttca attgctgctc gacgcgaccg gtcagtttgg ccatttggtc
       61 gacaacaccg ccgaattgga tgttcgattc aacccggcgt gggaggtgga gcaggcatga
      121 ctgcgccatg tgacctctcc atgatcgaag cccggcagcg gatgcaggac ggctccctta
      181 ccgccgtcgc cctgatggaa tcagtgctgg atcggatgcc ggccatcccg accgagtacc
      241 acccgtacgt cgtcatccgg gaccgtgaat cacttatggc cgacgcggcc gcggccgacc
      301 atgctcgggc gagcggcgag tcgaccggcc tgctgcatgg aatcccgatc gggatcaagg
      361 acatcgtcga cgtgtgcggg tttccgacca agtgcggttc ccgctcgatg gaggattgcg
      421 caccggcgat tgaggatgcg ccgctggtgg ccggattcaa ggccgccggc gggatcatcc
      481 tcggcaagac agtcacgcag gagttcgcgg ccggcacgat cagcgcgccc tcccggaacc
      541 cgtgggacct cgaccgtatc ccgggtggca gctccggcgg atcggcggca gccatcgcgg
      601 ccggcatggc gctgatgaca atcggcaccg atacaggtgg cagcatccgc tgtccggcct
      661 cggtctgcgg cgtcaccgga ctcaagccaa cctatggcac agtcagccgg cgcgggatct
      721 atcccctcgc gtggtcgctc gacacggccg ggccgatcgc gcggaccgtc gaggactgcg
      781 ccattgccct cgacaccatc gccggtcatg atccgcgtga tcccggctcc gcgccggtgc
      841 gtcacggcag cgcagcggcc gagatcgggc aggacattcg cggactcagg atcggcctgc
      901 cgcgggcgtt cttctacgac aagctcgagc cggagttgtc ggacatcttt gatcaggccg
      961 cccagtgcct gcgcgagctc ggcgcggacg tgatcgaggt tgattgggac ctggctctcg
     1021 aggcgcggtt ggtttcgatg gtaatcaacc gggccgagac gtcggcagtc cacgatgatc
     1081 gagtccggac gcacccggat ctcgtcggcg acgagtggcg actacgggtc aaggccggtc
     1141 tgctcttgcc ggcccgcaac tacatcacgg cgcaccaggc ccgcatcgtc gtccggcagt
     1201 cggtcgcgaa ttatttcaac cagaatcggc tcgacgcgat gatcgcgccg gcaacggccg
     1261 gcaccgctgc gcgcgccgac gatccattcc tgacctacac cgacggcagt cgcgagcacg
     1321 tcctgagcgg ctacacgcga atgacgatgc cgaccaatgc gacgggacag ccggcaatct
     1381 cggtcccggc cggcttcacg agcgccggct tgccgatcgg gatgcagatc atcggtcgac
     1441 ccttcgccga agcgcggatt tgccggatcg gccacgccta cgagcaggcg acgcgatgga
     1501 tcgatcgccg gccgccgctt acccagggag cctgagatgg atcagcagga tttcggaagc
     1561 gtcgcccgtc gcggcgcgca ggcacgggcc aacctcgttt cagcgcttcg tgagtgcagc
     1621 gagctggccg atgcggtcga gacgttccat gacgacgaac tgctcgaggt cctcgcgcac
     1681 ctcgacagcc tgcgattcgt gatggcggaa tctggccaaa tcctggccgg cgtgatccgc
     1741 ggtttcgact agggcggtgc ccggaagaag taccggttcc gaacatgtag ccccgggcgg
     1801 ctcgcccggc cgtgtcacgc caaaccgggg cgagccgccc gtggcgacat tggcaaggca
     1861 ggcacgtttt ctgaaacccg ctttaaagca gaggccgcaa ataatgtcac agtccgccgg
     1921 cgactcacgg ccccggctca ccaatggaag acgtcagatg gatttcgccg gctgcattgg
     1981 gtaattggaa cccgaaccgt gcc
//
LOCUS       DASSGX010000028         2558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1310644, whole genome shotgun sequence.
ACCESSION   DASSGX010000028 DASSGX010000000
VERSION     DASSGX010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1310644"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            323..>2558
                     /locus_tag="VFI12_00445"
     CDS             323..>2558
                     /locus_tag="VFI12_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7054892.1"
                     /translation="MDTTLRHDPAPPPLDLPAAPVVAPATDLMSHALVISGRPMIWTA
                     FRSKATEEALALARLLRWCLDIPGTHIGIDPRGLAQIMRGIALQGEAPPLTPTDDELL
                     ALAGAPKEGGPSWPTDGPHFASHWVAMLTGLRRVAGTSGSWWLDDADRVWWFEATTAS
                     LLAYAREIDVLPDETEMLPIMRRHEFCLFGRLNSDDLLQQLHMLFTAAPEPSRSDALK
                     QLAQFTSFVARSEDASALYWSWQLLNGSGVRESAINTPLLTLWNYWPRSRRFAPEPKA
                     GDLWESSPLATGDMAVFLQGAWEHAETMFTLARDVHGVSFSEPEVDRVTCLAPVMFVA
                     SPALPPMQPEDYLARNGRSILPLVRTCLGSDDARSQGTACGVLEGELLTLRREIKYQN
                     QVMPRYLVLPAASAALPSREAEDDLNRIAQILQYIEYDTASAARRLVSDIEPLADRQS
                     IWAGSLRDLSALLDEGLDLIQSVSPSDLQRIYERCCTFSLALGRLQARFARSSSGVNT
                     LVRTFQGEIAASEEYLQGRLTVTSVTGLGLQDLLPALLSAFPYQRTRRLLEETKNQTD
                     ALVDGMERLRQLTETVLREAERLGKARLAIMSGLLTGLVPALALMIALPGFIPDAQLR
                     RDTYPQWLEGRLGLTALAVGFQSVALIMLVVSLVVLGLYAMARLSARPGVLSPYVAKA
                     QQFTKAVAEAEANCREARLREHIDENDLSGWAAVAANDLAAAPLLDALWKDVYQANER
                     LSWPR"
BASE COUNT          388 a          879 c          857 g          434 t
ORIGIN      
        1 agatcggtca ggagaccggt gatcgcctca gagtcgggcc acatgtgctc cacgtccccc
       61 gcgtggtagc gcgaccgccg cgatgacggc cgcgcaatcc gcatccattc cggtgcgaca
      121 tggtacaggg cgctgggcgt caccgcacgg ctcgggatca tcgccaaggc gggtattgtg
      181 tcgagacgtg ccgggtgata ggatcatgca atctgttgca tgtcgttgcc ccgaggccac
      241 cggaccgagc gcgctggcgg cgcgggtgtc tccgggagag agaaacgagg cccgatgaac
      301 tgaaccgggt ggagcccctc ctatggacac gacgctgcgc cacgaccctg cccctcctcc
      361 gctcgatctc ccggctgcgc ccgtggtcgc gccagccacc gatctgatga gtcacgccct
      421 ggtgatctcg gggcgtccga tgatctggac cgcgtttcgc tccaaggcga ccgaggaggc
      481 gctcgcgctg gctcgtctcc tccgctggtg cctcgacatt cctggcacgc acatcggtat
      541 cgatccacgc ggtctcgcgc agatcatgcg cggcatcgcc ttgcagggcg aggcgccgcc
      601 actcacgccc acagatgacg agctgttggc cctggccggc gcgccgaagg agggagggcc
      661 gtcctggccg accgatgggc cgcatttcgc ttcccattgg gtggcgatgc tgaccgggct
      721 gcgccgcgtg gcaggaacgt ctggctcctg gtggctcgat gacgccgacc gggtctggtg
      781 gttcgaggcg acgacggcca gcctccttgc ctacgcccgg gagatcgacg tcctgccgga
      841 cgaaaccgaa atgctgccca tcatgcggcg ccacgagttc tgcctcttcg gcagactgaa
      901 cagcgacgac ctcctccagc aattgcacat gctcttcacc gcggcgccgg aaccgagccg
      961 gtccgacgcg ttgaaacagc tcgcgcagtt cacatcattc gtcgcccgct ccgaggacgc
     1021 cagcgcgctc tactggagct ggcagttgct caatggctcg ggggtgcgcg agagcgcgat
     1081 caacacgccg ctgctgaccc tctggaatta ctggccgcgc agtcgccgct tcgcgccgga
     1141 gccgaaggcc ggcgacctct gggaatcgtc tccgctcgcc acgggtgaca tggcggtctt
     1201 cctgcaaggc gcctgggaac atgccgagac gatgttcacc ttggcccgtg acgtgcacgg
     1261 cgtctcgttc tcggagccgg aggtcgaccg cgtcacctgc ctggcgccgg tgatgttcgt
     1321 cgccagcccc gccctcccgc cgatgcagcc cgaggattac ctggcgcgca acgggcgatc
     1381 gatcctcccg ctcgtcagaa cctgcctcgg ctccgatgac gcccgctccc aagggaccgc
     1441 ctgcggcgtg ctggaggggg aattgctgac cctgcgccgc gagatcaagt atcagaacca
     1501 ggtcatgccg cgctacctcg tcctgccggc ggcgtccgcc gccttgccct cgcgcgaggc
     1561 ggaagacgat ctcaaccgca tcgcccagat cctgcagtac atcgaatacg ataccgcctc
     1621 ggctgcgcgc cgcctcgtct cggacatcga gccgctggcc gaccggcaat cgatttgggc
     1681 cggttcgctg cgcgatctga gcgcgctcct ggacgagggg ttggacctga tacagtcggt
     1741 ctcgcccagt gacctgcagc gcatctacga gcgttgctgc acgttctccc tcgccctggg
     1801 gcggttgcag gcgcgctttg cccgctccag cagcggcgtg aacaccctag tgaggacgtt
     1861 tcaaggggag atcgccgcgt cggaggagta cctgcagggc cgtctgaccg tgacgtcggt
     1921 gacagggctg gggctgcagg atctgctgcc cgcgctcctg agcgcgtttc cctatcaacg
     1981 gacgcggcgg ctcctcgagg agacgaagaa ccagaccgat gcgctcgtcg acgggatgga
     2041 gcgcctgcgc caactgacgg agacggtgct gcgggaggcg gagcggctgg gcaaagcgcg
     2101 gctggccatc atgtccgggc tcctcaccgg cctcgtgccc gcgctggcgc tcatgatcgc
     2161 cctgcccggc ttcatcccgg acgcgcaact gcggcgcgac acctatcctc agtggttgga
     2221 ggggcgcctc ggcctcaccg cgctcgcggt cgggttccag tcggtggcgc tcatcatgct
     2281 cgtggtctct ctcgtcgtcc ttggtctgta cgcaatggcg cggctcagcg cgcggccggg
     2341 cgtactctcc ccgtatgttg ccaaggcgca gcagttcacc aaggccgtcg ccgaggcgga
     2401 agcgaactgc agggaggcgc gtctgcgcga gcacatcgac gagaacgacc tctcaggctg
     2461 ggccgcagtg gcagccaatg atctggcggc ggcgccgctg ctggacgcgc tctggaagga
     2521 tgtctatcag gcgaacgagc ggctgtcgtg gccgcggc
//
LOCUS       DASSGX010000029         2648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1092179, whole genome shotgun sequence.
ACCESSION   DASSGX010000029 DASSGX010000000
VERSION     DASSGX010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092179"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /locus_tag="VFI12_00450"
     CDS             <1..563
                     /locus_tag="VFI12_00450"
                     /inference="COORDINATES: protein motif:HMM:NF014047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HET7054893.1"
                     /translation="MRTTFIVGYPGETEEQFERLLAFVEAEEFDHVGVFTYSPEPGTV
                     AADLPNHIPSAIAEERRGRIMEAQQKISYRKNRAMVGSTLQVLIEGVGEIENEDGSSE
                     PVAVGRARRHAPEVDGLVFVPGTVPVGEMIDVTITDASPYDLWGAPAGMERLVAESRP
                     VIRRPSHLSKVRRRSRPVPMAKLTGT"
     gene            complement(514..1533)
                     /locus_tag="VFI12_00455"
     CDS             complement(514..1533)
                     /locus_tag="VFI12_00455"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7054894.1"
                     /translation="MAIRVGITGQEPVQNQDAVPEKPSTLARKVSERFRREDPHYIRH
                     FISIMVARVSSRLARSLPVGLRYFLADRLGDLLYLISPGYRRNVVSNLRHVHSGSGLP
                     QPTKRDVHNIFRVSARNWADLLVVPKRSTDQFRSDVRVTPGSLERLDAALARGKGCIL
                     ITAHLGAFDFMGHYLHAVGYKLTIVTGRTTARIVFDGVTYIRQSNGLPLVEATPSGVR
                     RAIQAVRQGECAVIVMDRDFFQNGIRAKFFGEETTLPPGAIRIARDTGASIVPIYAKR
                     VAEGHEVTIHEPFTIERTPDLQHDLEGGMGKVVDSLETALSRVPDQWVMFQSVWPSGP
                     GDSAV"
     gene            complement(1572..2225)
                     /locus_tag="VFI12_00460"
     CDS             complement(1572..2225)
                     /locus_tag="VFI12_00460"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET7054895.1"
                     /translation="MANLITIGRLVLLFVVIGMIYWGSAGAILVAILLVALVFAGDGI
                     DGYVARRTNSTSQFGAVFDIAGDRIVENALWVIFAERQLIPVWIPLVVLTRGFIVDGI
                     RSISYAEGMTAFGERNMMRSSFTRWLTAGRFMRTLFGWTKAFGFLFLTGLEGYRHRNA
                     PDSLIHQMYDWDPLRWAGWACVVVAVALTIVRGLPVIYDATALWSKPTAAVQDATRE"
     gene            complement(2271..>2648)
                     /locus_tag="VFI12_00465"
     CDS             complement(2271..>2648)
                     /locus_tag="VFI12_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7054896.1"
                     /translation="PRIDQQRLRDVSAVFANPAPEVRPLMMGGLAAVASLILLSILVL
                     VKSGSMPGLIPLHLNAEGTTTLFGTKGSVWRLPFFALLTTIMAFGLGWWLRTRDAFAV
                     QYLVVGALMIHCLIWVGAINLLW"
BASE COUNT          510 a          836 c          862 g          440 t
ORIGIN      
        1 cgatgcgcac gacattcatc gtcgggtatc ccggcgagac cgaagagcag ttcgagcgat
       61 tgctcgcatt cgtcgaggcc gaggagttcg atcacgtcgg cgtcttcacc tattccccgg
      121 agccaggcac ggtcgcggcc gacctgccga atcacatccc ctctgcgatt gcggaggagc
      181 gccgcggccg catcatggag gcccagcaga agatctccta tcgcaagaat cgggcgatgg
      241 ttggttcgac gctacaggtc ctgatcgaag gcgtcggtga aatcgaaaac gaggacggat
      301 cgagcgagcc agtggcggtc ggtcgcgctc gccgccatgc gccggaggtc gacgggctgg
      361 tcttcgtgcc ggggactgtt ccggtgggcg agatgatcga cgtgacaatc actgatgcga
      421 gtccgtacga tctctggggc gcgccggcgg ggatggagcg actcgtggct gaatcccgcc
      481 cagtgatccg ccgtccgtcg cacctgtcga aggtcagacg gcggagtcgc ccggtcccga
      541 tggccaaact gactggaaca tgacccactg atcggggacc cgcgatagcg cggtttccaa
      601 cgagtcgacg actttgccca tgccgccttc gaggtcgtgt tggaggtccg gcgtccgctc
      661 gatcgtgaac ggctcgtgga tcgtaacctc gtggccctcg gcaacccgct tcgcgtagat
      721 cgggacgatt gacgccccgg tgtcccgcgc gattcggata gcgccgggcg gcagcgtcgt
      781 ctcctcgccg aaaaatttgg cccggatgcc attctggaag aagtcgcgat ccatgacgat
      841 cacggcgcac tccccttgcc tgaccgcctg gatcgcgcgg cgcacgcctg atggtgtcgc
      901 ctcgaccagc ggcagcccgt tcgactggcg aatataggtc acgccgtcga atacgatccg
      961 ggccgtggtt cgcccggtca cgatcgtcag cttgtaccct actgcgtgca ggtagtgacc
     1021 catgaagtcg aacgcgccca ggtgagccgt gatcaggatg cagcccttgc cgcgcgcgag
     1081 cgctgcgtcg agtcgttcga gcgaccccgg cgtcacgcgc acgtcgctgc ggaactggtc
     1141 cgtgctgcgt tttggcacta ccagcaagtc cgcccaattg cgggccgaga cacggaagat
     1201 gttgtgcaca tcgcgcttcg tcggctgggg aagtccgctg ccggaatgca cgtgacggag
     1261 attcgacacc acgttccgcc ggtatccggg gcttatcagg tagagaagat cgccgaggcg
     1321 gtcggcaagg aaatagcgaa ggccgaccgg gagggaccgc gccagccgtg agctgacccg
     1381 cgcaaccatg atgctgatga aatggcgaat gtaatgcggg tcctctcggc ggaaccgctc
     1441 gctgactttg cgcgccagcg tcgacggctt ctcaggcacg gcgtcctggt tctgtactgg
     1501 ttcctggccg gttatgccca ctcggatggc catggtgctc gttcattctg cagtccgttc
     1561 cagccagccg gtcattctcg ggtcgcgtcc tgaaccgcag ccgtcggttt gctccagagc
     1621 gcagtcgcat cataaatcac cgggaggcca cgaacgatgg tcagggcaac cgctaccacc
     1681 acgcatgccc aaccggccca gcgaagcggg tcccagtcgt acatctggtg aatcagggaa
     1741 tccggcgcgt tgcgatgccg gtagccttcg aggccggtga gaaacaagaa cccgaacgcc
     1801 ttggtccagc cgaagagggt ccgcatgaat cgcccggccg tcagccaccg cgtgaaggac
     1861 gagcgcatca tgttccgctc gccgaatgcg gtcattcctt ccgcatagga gatggaacga
     1921 atgccgtcga cgatgaagcc gcgcgtcagc acgaccagcg ggatccagac cgggatgagt
     1981 tgccgctcgg caaagatcac ccagagggcg ttctcaacga tccggtcgcc ggcgatgtcg
     2041 aacacggcgc cgaactggga ggtggaattt gttctccgcg ccacgtaccc gtcgatgccg
     2101 tctcccgcga agaccagggc gaccaggaga atcgcaacga ggatggcgcc ggcgctgccc
     2161 caatagatca tgccgatgac gacgaacagc aagacaagcc gcccgatcgt gatcaggtta
     2221 gccacccact cctcctgccc gatctaccgg ggccacgggc accgcacgcg ctaccacaga
     2281 agattgatcg ccccgaccca gatcaggcag tgaatcatga gcgcgccgac gacgaggtat
     2341 tgaacggcga aggcatcccg ggtgcggagc caccagccga ggccgaaggc catgatcgtc
     2401 gtcaggagcg cgaagaacgg gagccgccag accgaaccct tcgtgccgaa gagggtcgtc
     2461 gtgccttccg cgttcagatg caacgggatc aggccgggca tggagcccga cttcacgagc
     2521 acaaggatcg agagcaggat cagagaggcg actgccgcga gcccgcccat cattaatggg
     2581 cgaacctcag gcgcgggatt ggcaaagacc gcgctgacgt ctcggaggcg ctgctggtcg
     2641 atccgcgg
//
LOCUS       DASSGX010000030         2424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_222211, whole genome shotgun sequence.
ACCESSION   DASSGX010000030 DASSGX010000000
VERSION     DASSGX010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222211"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VFI12_00470"
     CDS             <1..598
                     /locus_tag="VFI12_00470"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7054897.1"
                     /translation="GLIAALIAAVIGVVIGSISGFFGGWLDAVLSRFTDVFLIMPAFF
                     LIIIVVATLGNSLFYVMVVIGLTTWPGNARLMRAQVLTLRERQYIQSLTALGESRWRI
                     LARHIIPNGIQPIIANSTLLIAAAILTEAGLAFLGLGDPNKASWGRMINDGRPSILTA
                     WWPSVFAGLAMIVTVLAFYLIGDGIAAAIASGGERDRE"
     gene            595..1581
                     /locus_tag="VFI12_00475"
     CDS             595..1581
                     /locus_tag="VFI12_00475"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7054898.1"
                     /translation="MSAPLLRLDRLDVDYRTSSGNISAVQGVSLEVPKGTTVGLVGES
                     GSGKSTIAAAILRLLPRNATVSPQSIFFDGVDLGTLSDKELSQKWRWREMAMVFQRSL
                     SALSPVHRIGAQLDDVLRINEPELSKHQRAERLEHLLKTVQLPERVLRSYAFELSGGM
                     MQRTMIAFGLLCSPPFLIFDEATTALDVITQAQILREVNRLQQEFDLTAVVISHDVGV
                     INATSNVVAVLYAGQLMEVAPRQRFFTAPAHPYSKALIESVPRVSGTSGRLTAIPGSL
                     PDLKHPPPGCRFAPRCTFAMPECSAAIPPLYQLEDGHLVRCVLYKDNPVEAP"
     gene            1593..>2424
                     /locus_tag="VFI12_00480"
     CDS             1593..>2424
                     /locus_tag="VFI12_00480"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7054899.1"
                     /translation="MLTVTDLRCLFPTQRTLNDRMNGRHPAVHAVDAVSFTLDRAEVL
                     AVVGESGCGKSTTGRMLVRLIEPTGGSIDFDGEDVTRLSGDALKQFRRKAQIIAQNPF
                     EAFDPRQTVQASLEQPLRIFGIGQPSERVGLIEAALEKAGLTPAADFLPRLPHELSGG
                     QLQRIATVRVTLLEPRFIVADEPVSMLDVSVRADILNHFLDLRDETGLAILFITHDIA
                     VARYVASRIAVMYLGMFVETGTTEDVIDQPKHPYTRALLSNTLSVEGDRAGGDPIEIR
                     G"
BASE COUNT          410 a          837 c          730 g          447 t
ORIGIN      
        1 cgggctcatc gccgcattga tcgcggcggt gatcggcgtg gtcatcggca gcatctccgg
       61 cttcttcggt ggctggctcg atgcggtgct cagccgcttc accgatgtct ttctcatcat
      121 gccggccttc tttctgatca tcatcgtggt ggcgaccctg ggcaacagcc tcttctacgt
      181 tatggtcgtg atcggcctga cgacctggcc gggcaatgcc cggctgatgc gcgcgcaagt
      241 gctgaccctg cgcgagcggc aatacatcca gtcgctgacg gcgctgggag aaagccgctg
      301 gcgcattctg gcgcggcata tcatcccgaa cggcatccaa ccgatcatcg ccaacagcac
      361 cttgctgatc gccgcggcga tcctgaccga agccggcctg gcgttcctcg ggttgggcga
      421 tccgaacaaa gcctcctggg gtcgcatgat caacgacggc cgcccctcga ttctgaccgc
      481 ctggtggccg tcggtctttg ccgggctggc gatgatcgtc accgtgctcg ccttctatct
      541 gatcggggac ggaatcgccg cggcgatcgc ctcgggcggg gagcgcgacc gcgaatgagc
      601 gcgccattgc tacggctcga ccggctcgat gtcgactacc gcaccagcag cggcaacatc
      661 agcgcggtgc agggcgtgtc gctcgaggtg cccaaaggca cgacggttgg tctggttggc
      721 gagagcggtt cgggcaaatc gaccatcgcc gccgcaattc tgcgcctgct gccgagaaac
      781 gcgactgtct cgccgcagtc gatctttttc gacggcgtcg atctggggac gctctccgac
      841 aaggagctca gccagaaatg gcgctggcgc gagatggcga tggtcttcca acgctcgctc
      901 tccgccctca gcccggtgca ccgcatcggc gcccagctcg acgatgtgct gcgtatcaac
      961 gagccggagc tctccaaaca ccaacgcgcc gagcggctgg agcacctgtt gaaaaccgtg
     1021 cagctccccg agcgggtgct gcgctcctac gccttcgagc tctccggcgg catgatgcaa
     1081 cgcaccatga tcgccttcgg tctcctgtgc agtccgccct ttctgatctt cgacgaagcc
     1141 accaccgcgc tcgatgtcat cacccaggcg cagatcctgc gcgaagtcaa ccggctgcaa
     1201 caggaattcg atctgaccgc cgtggtcatt tcgcacgatg tcggggtgat caacgccacc
     1261 tcgaatgtcg tcgcggtgct ctacgccggg cagctcatgg aagtcgcgcc gcggcaacgc
     1321 ttcttcaccg ccccggccca tccctattca aaagcgctga tcgaatcggt gccgcgcgtc
     1381 tcgggcacgt ccggccggct aaccgctatt cccgggtcgc tgccggacct gaaacatccg
     1441 ccgcccggtt gccgtttcgc accccgctgc acattcgcca tgccggagtg ctccgcggcc
     1501 atcccgccgc tctaccagtt ggaagacggt cacctcgttc gctgcgtgct ctataaagac
     1561 aacccggtag aagcgccatg agcgaccagc ccatgctcac cgtcaccgac ctgcgttgcc
     1621 tctttcccac gcagcgcacg ctgaatgacc gtatgaacgg gcggcatccc gccgttcacg
     1681 cggtcgatgc agtctccttc acgctcgatc gcgccgaggt tctggcggtg gtgggcgaaa
     1741 gcggctgcgg caaatcgacc accggccgca tgctggtgcg tctgatcgaa ccgaccggcg
     1801 gctccatcga cttcgatggg gaagacgtca ccaggctgag cggtgacgca ctgaagcaat
     1861 tccgccgcaa ggcgcagatc atcgcccaga accccttcga ggcattcgat ccgcggcaaa
     1921 cggtgcaggc ttcgctcgag cagccattac ggattttcgg tatcgggcaa ccgtccgagc
     1981 gggtcggcct gattgaagcc gcgctggaaa aagccgggct cacccccgcc gccgatttcc
     2041 tgccgcggct tccgcacgag ctctccggcg gccagcttca gcgcatcgcg accgtgcggg
     2101 taaccctgct ggaaccgaga ttcatcgtgg ccgacgaacc ggtgtcgatg ctcgatgtct
     2161 cggtgcgggc cgatatcctc aatcatttcc tcgatctgcg cgacgagacc gggctggcga
     2221 tcctcttcat cacgcacgat atcgccgtgg cgcgctatgt cgcctcccgc atcgcggtga
     2281 tgtacctggg gatgttcgtc gaaaccggca cgaccgagga cgtcatcgac cagccgaaac
     2341 acccctacac ccgcgcgctc ctttccaaca ccctgagcgt cgaaggggac cgggccggcg
     2401 gtgaccccat cgaaatccgg ggcg
//
LOCUS       DASSGX010000031         2531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_875279, whole genome shotgun sequence.
ACCESSION   DASSGX010000031 DASSGX010000000
VERSION     DASSGX010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875279"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="VFI12_00485"
     CDS             <1..649
                     /locus_tag="VFI12_00485"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7054900.1"
                     /translation="FGEGEPLYGPITTADRYYAPEVEQYNQFDLDKAKSLVAELGWAA
                     NGDGWLEKNGTVLEFNLVVQAESLNQQFGQVLQDQLKQIGMKVNVVSLDRGTFFGDLG
                     SENGVDSHLFYWAWPVPADILQIFVSTATMGGPNWSYASVPAVDDAIDAWHTAANEDE
                     LRAAAKQLQLAVAENLPIVSLVNRNSVWVNRKTVHGYLPHQWNLYPYYNDVWLEQ"
     gene            1178..2098
                     /locus_tag="VFI12_00490"
     CDS             1178..2098
                     /locus_tag="VFI12_00490"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7054901.1"
                     /translation="MATYIIRRSLRMAIILVLASIAVFYALRIAPGDPSGTQLNAAAT
                     QQMREAYRERQGLNAPIWKQYFVYMGNLAQGDFGKSLVTGKGMIDLLKEYGKNSLILG
                     LTAFVLTHLLGVPLGIIAAARRNSIIDGVISSFSVLGMGIPNFWLALLLILLFSSKLH
                     WLPSAGCCGVNYLIMPAIVLAAEGTAVTVRMMRSSMLEQLGQDFVRTHRSKGLSEWTI
                     IGRRVSRTALIPVVSLAGLRLGWIVGYSLIVETIFRWPGVGYLLVDAVIRRDYPVAQF
                     FSLLLVVVVVVSNLLADLAYGVVDPRIRTR"
     gene            2095..>2531
                     /locus_tag="VFI12_00495"
     CDS             2095..>2531
                     /locus_tag="VFI12_00495"
                     /inference="COORDINATES: protein motif:HMM:NF024313.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7054902.1"
                     /translation="MIAAETTDAGLQTSDIRRAPRFVGFGRRLVRNPQALIGLVICLV
                     YFVIAAIGPALTSHDYETQNLMGTYLRPLSAGHILGTDDLGRDLYARLATGIRISLLV
                     GFGVTAIAMLVGIFFGILAGYFRGWVDMVVSGVVEFTWSFPLIL"
BASE COUNT          406 a          792 c          844 g          489 t
ORIGIN      
        1 cttcggcgag ggcgagccgc tctatggccc gatcacgacg gccgaccgct actacgcgcc
       61 cgaggtggag cagtacaacc agttcgacct ggacaaggcg aaatcgctgg tcgccgaact
      121 gggctgggcc gcgaatggcg atggctggct tgagaagaac ggcacggtcc tcgagttcaa
      181 tctcgtcgtc caggcggagt cgctcaacca gcagttcggc caggtgctcc aggaccagct
      241 caagcagatc gggatgaagg tcaacgtcgt ctcgctcgac cgcggcacct tcttcggcga
      301 tctcggctcg gagaacggcg tcgattcgca cctcttctac tgggcgtggc cggttccggc
      361 ggatatcctc cagatcttcg tcagcaccgc cacgatgggc gggccgaact ggagctacgc
      421 gagcgtgccg gccgtggacg atgcgatcga cgcctggcac acggccgcca acgaagatga
      481 gctgcgggcc gcggccaagc aattgcaact ggcggtggcg gagaacctgc cgatcgtctc
      541 gctggtcaac cggaacagcg tctgggtcaa ccggaagaca gtccacggct atctgccgca
      601 ccagtggaac ttgtatccgt attacaacga tgtgtggttg gagcagtagc gaggcgagaa
      661 ggcgagcagt cgggaggcga gcaggcgagt agctaccgaa ctcccctctt ccgtcagaaa
      721 gaagggcggg agaggggctg gaggtgagag ttactgcctg gttcggtgat cggtctcccg
      781 gcgtcgcccg gattgaaatc cgggcccagg gtccggatgt ccctccggga cagggtgtcg
      841 tttgcgccat gaaatggcac tcgtcgccgg cgcggcgcct gtcggtttca accgacatcc
      901 gtacgctagc ccgggaattc attcccgggc catgccggaa cgccggttga tacgcaatgg
      961 ccgcactggg gcccgaattc gcgtcgttcg gcgataccat cgtggcctac ggccgccgcg
     1021 gggcatggta cccccgtgct acgttttggc attcgtcctc gtgtcacgag ctcccaggct
     1081 cggagaggat ccatgtatta agcccggggg ttcaaccccg ggaaccaaac ccggcccggt
     1141 ggaatcgggg gcggttggtt tgtgcggagg gcattgattg gcgacatata tcatccgccg
     1201 gtcattgcgg atggcgatca tcctggtcct ggcctcgatc gccgtcttct atgcgctgcg
     1261 aatcgccccc ggcgacccaa gcggcacgca gcttaatgcg gccgcgacgc agcagatgcg
     1321 cgaggcctac cgtgagcggc aggggctgaa cgcgccgatc tggaagcaat acttcgtcta
     1381 catgggcaac ctcgcccagg gtgacttcgg gaaatcgctc gtcaccggca agggcatgat
     1441 cgacctgctc aaggagtacg gcaagaacag cctgatcctc gggctgaccg ccttcgtcct
     1501 gacccatttg ctcggcgttc cgctcgggat catcgccgcc gcccggcgca actcgatcat
     1561 cgacggcgtc atctccagct tcagcgtgct cgggatgggc atccccaact tctggctggc
     1621 gctgctcctg atcctgcttt tttcctcgaa gctgcactgg ctgccgtcgg ccggctgctg
     1681 cggcgtcaat tacctgatca tgccggcgat cgtcctcgcg gccgagggga cggccgtcac
     1741 ggtccggatg atgcgctcct cgatgctcga gcagctcggg caggacttcg tgcggacgca
     1801 tcgatccaag gggttgagcg aatggacgat catcggccgc cgcgtctcgc gcacggcgct
     1861 gatcccggtc gtctcgctgg ccgggctgcg gcttggctgg atcgtcggct actcgctgat
     1921 cgtcgagacg atcttccgct ggccgggcgt cgggtatctg ctggtcgatg ccgtcatccg
     1981 gcgcgactac ccggtggcgc agttcttctc gttgctcctg gtggtggtcg tcgtggtctc
     2041 gaatctgctg gccgatctgg cctacggcgt ggttgacccg cgcatccgga cacgatgatc
     2101 gcggcggaga cgacagacgc cgggctccag acgtcggaca tcaggcgggc gcctcgattc
     2161 gtcggtttcg ggcggcggct ggttcgcaat ccacaggcat tgatcggcct ggtgatctgc
     2221 ctcgtctatt tcgtgatcgc ggcgatcggc ccggcgctga ccagccatga ttacgagacg
     2281 cagaacctga tgggcaccta tctgcgcccg ctcagcgccg gtcacatcct cggcaccgac
     2341 gacctcgggc gggacctcta cgcgcggctg gcgaccggga tccggatctc gctgctggtc
     2401 gggtttggcg tgaccgcgat cgcgatgctg gttgggatct tcttcgggat cctggccggg
     2461 tacttccggg gctgggtcga tatggtcgtc agcggtgtgg tcgagttcac ctggagcttc
     2521 ccgctgatcc t
//
LOCUS       DASSGX010000032         3224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_658396, whole genome shotgun sequence.
ACCESSION   DASSGX010000032 DASSGX010000000
VERSION     DASSGX010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658396"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1373
                     /locus_tag="VFI12_00500"
     CDS             <1..1373
                     /locus_tag="VFI12_00500"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7054903.1"
                     /translation="DVVMEEVEQILDLGIDVRFNAEVGKDISFKDMVDSFDSVLISAG
                     CQTAQGLGIPGEELEGVVSGLQFLEDVNLGVKDVWVGKHCVTVGGGFTSMDCVRTVMR
                     MGAETSIMTYRRTIQEIPVEELELDEAEIEGVEVMYLITPIEVIGENGKAVGLKCQKN
                     QLGAPDKQGRRRPEPIPGSEFVIPCDMILVAIGQRQDSSFLGDLLPNRDRRGVPFLDQ
                     NLRTELPNVWASGDYVVNPTNFISSIGEGKRVADLIDQQLRGTKPRVREMELTRVPTE
                     FVATPNPLMAEGIAEWSLTAMSRRLVWGDDYAGVGRQVMPSLAVGDRGIGTLDTTLEV
                     EVGMTKPIGFEEAKRCLQCQLNIFIDGNRCILCNGCVDACPHRCIEMISPERIYAIDN
                     DVELAEMARAELGPFAAAMVIDERSCIRCGICVDWCPTECLTMDHFRVTPPETRESVD
                     LAIVAD"
     gene            complement(1455..2342)
                     /locus_tag="VFI12_00505"
     CDS             complement(1455..2342)
                     /locus_tag="VFI12_00505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7054904.1"
                     /translation="MPDSDWLASQFEDHRDRLRAVAYRVLGSRSEAEDAVQEAWLRLS
                     RTDTSDVENFGGWLTTVVSRISLDMLRSRGSRREDRLGPQDFDGIVNDSVESEPERAA
                     VLADSVGPALLVVLETLTPAERLAFVLHDVFGVPYEEIAPIIERNPAAARQLASRARR
                     RVRGASMVTQSADQVRRYAIVDAFLAAARDGDFDALIGFLDPNVVLRADQTAVEMGTA
                     PEIRGPREVAGRFVGGAKVARTAIVDGAAGAVWMSGKQPRIAFRFTFMADLIAAIEIV
                     ADPARLATLDIALRHPARD"
     gene            2427..2771
                     /locus_tag="VFI12_00510"
     CDS             2427..2771
                     /locus_tag="VFI12_00510"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7054905.1"
                     /translation="MTSGLKTIIYPVSDLAGAKARFTTLFGVEPYIDQPYYVAFNVDG
                     NDVGLDPNGHAKGMTGALPYWHVADIRATIDQLVAAGADPQQEPTDVGGGNLIATVTD
                     TDGNTIGLMQAA"
     gene            2805..3221
                     /gene="tsaA"
                     /locus_tag="VFI12_00515"
     CDS             2805..3221
                     /gene="tsaA"
                     /locus_tag="VFI12_00515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HET7054906.1"
                     /translation="MSDPEYLVRPVGWVASPLTDRSAAPRQGDEGAPTAQIVIDARFR
                     EAAQDLRPGDKILLLTWLHLGDRTTLAVHPRGDKSRPITGVFSTRLPDRPNPIGLHAV
                     RIESIAEGAITVRNLVAVDGTPVLDIKPVLRDPRER"
BASE COUNT          597 a         1067 c         1010 g          550 t
ORIGIN      
        1 gtgacgtcgt catggaagag gtcgagcaaa tcctcgatct cggcatcgac gtccgtttca
       61 acgcggaggt cggcaaggac atctccttta aggacatggt cgatagcttc gactctgtcc
      121 tgatctcggc cggttgtcag accgcgcagg gcctcggcat cccgggcgag gagctcgaag
      181 gcgtcgtctc cggtctccag ttcctcgaag acgtcaacct cggcgtcaag gacgtctggg
      241 tcggcaagca ctgcgtcact gtcggcggtg gcttcacctc gatggactgc gtccgcaccg
      301 ttatgcggat gggcgccgag acctcgatca tgacctaccg ccggacgatc caggagatcc
      361 cggtcgaaga gctcgagctc gacgaggccg agatcgaggg cgtcgaggtc atgtacctga
      421 tcaccccgat cgaagtgatc ggcgagaacg gcaaggccgt cgggctcaag tgccagaaga
      481 accagctcgg cgcgcccgac aagcaaggcc gtcggcggcc ggaaccgatt cccggctccg
      541 agttcgtgat cccgtgcgac atgatcctcg tcgccattgg ccagaggcag gacagcagct
      601 tcctcggcga tctgctcccg aaccgggatc gccgcggcgt gccgttcctc gaccagaacc
      661 tgcgcaccga gctgccaaac gtttgggcgt ctggcgacta cgtcgtcaat ccgaccaact
      721 tcatctcctc gatcggcgag ggcaagcgag ttgccgacct gatcgaccag cagctccggg
      781 gcacgaagcc gcgcgtccgc gagatggagc tgacgcgcgt cccgactgag tttgtcgcga
      841 cgccgaaccc gctgatggca gagggtattg ccgagtggag cctgacggcg atgagccgcc
      901 gcctcgtctg gggcgacgac tacgccggcg tcggccggca agtgatgccg tcgctcgcgg
      961 tcggggatcg cggtattggc acgctcgata cgaccctcga agtcgaagtc ggcatgacca
     1021 agccgatcgg gttcgaggag gccaagcgct gtctccagtg ccagctcaac atcttcatcg
     1081 acggcaaccg ctgcatcctc tgtaacggct gcgtcgatgc ctgcccacac cgctgcatcg
     1141 agatgatctc gccggagcgg atctacgcga tcgacaacga cgtcgagttg gcggagatgg
     1201 cccgcgccga gctcggaccg ttcgctgccg cgatggtgat cgacgagcga agctgcatcc
     1261 gctgcggcat ctgcgtcgac tggtgcccga ccgagtgcct gacgatggat cacttccgcg
     1321 tcacgccgcc ggaaacgcgc gaaagcgtcg acctcgcgat cgtcgccgac tgagccggta
     1381 agcgccggcc aagcacggca ggagcatccc tcggaccaat atgaggtccg agggatttcc
     1441 gttcctgatg gatttcagtc gcgtgccggg tgtcgaagag cgatatcaag cgtcgcgagg
     1501 cgcgccggat ccgcaacgat ctcgatcgcc gcgatcaaat cagccatgaa cgtgaagcga
     1561 aatgcgatgc gaggttgttt gccggacatc cagaccgcgc ccgccgcgcc atcgacgatc
     1621 gcggttctgg cgaccttggc tcctccgacg aaccggcctg cgacctccct gggaccgcga
     1681 atctccggcg ccgtccccat ctcgacggct gtctggtcag cgcgcaagac cacgttcgga
     1741 tccagaaagc cgatcagagc gtcgaaatca ccgtctcgcg ctgccgcgag gaaggcatcg
     1801 acgatggcgt accgacggac ctgatcggcg ctctgcgtca ccatcgacgc gccgcgtact
     1861 cgccggcggg cgcgactggc gagctgcctg gcggcggctg gattacgttc gatgatgggc
     1921 gcgatctcct cgtacggtac gccgaagacg tcgtgcagga cgaaagcaag ccgttcggcc
     1981 ggtgtcagag tttccaacac cacgagcaac gccggtccga ccgaatcggc cagcaccgcc
     2041 gcgcgctccg gttcgctctc aacgctgtcg ttgacgatcc cgtcgaaatc ctgcggtccc
     2101 agccgatcct cccggcgcga tccacgcgag cgcaacatgt cgagcgatat gcgggacacc
     2161 accgtcgtca gccagccgcc gaagttctca acgtcgctgg tatcggtccg gctcaaccgc
     2221 agccatgcct cctgcacagc gtcctctgct tcgctgcggg agcccagcac ccgataggca
     2281 acggcccgca ggcgatcccg gtgatcttcg aattggctcg ccaaccagtc gctatccggc
     2341 attggtcaca ctttccgctc acggtgcgtc atagagttga cggccgaact tcggtcgatg
     2401 tgacatcgca tgatgaggag tgtttcatga catccggtct caagacgatc atctatccgg
     2461 tttctgatct tgccggcgca aaggctcgct tcacgacgct attcggcgtc gagccgtata
     2521 tcgaccagcc atattacgtc gcgttcaacg tcgatgggaa cgacgtcggt ctcgacccga
     2581 acggtcacgc caagggcatg accggcgcgc tcccctactg gcacgtggcc gacatcaggg
     2641 ccaccattga ccagctcgtc gccgccggcg cggacccgca gcaggagccg accgacgtag
     2701 ggggcggcaa tttgatcgcg acggtcacag acacggacgg taatacgatc ggcctcatgc
     2761 aggcggcctg aggtcgcgtt tggcgatagc agagggagca gagcgtgagc gatccggaat
     2821 atctcgtgcg gcctgtcggt tgggtcgcgt ccccgctgac cgaccgttcc gccgctccca
     2881 ggcagggaga cgagggcgct ccaacggcgc aaatcgtgat cgacgcccgg tttcgcgagg
     2941 ccgcacaaga tcttcgccct ggcgacaaga tcctgctcct tacctggctg cacctcggtg
     3001 accgcacgac gcttgccgtc catccgcgcg gcgacaagag tcggccgatc accggggtct
     3061 tctcaacgag attgcccgat cgcccaaacc cgatcggact gcacgccgtc aggatcgaat
     3121 cgattgcgga gggcgcgatc actgtccgca acctcgtggc ggtcgacggg acaccggtcc
     3181 tcgacatcaa accggtactc cgcgatccgc gcgagcgatg aacg
//
LOCUS       DASSGX010000033         4202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_114173, whole genome shotgun sequence.
ACCESSION   DASSGX010000033 DASSGX010000000
VERSION     DASSGX010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4202
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114173"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..722
                     /locus_tag="VFI12_00520"
     CDS             234..722
                     /locus_tag="VFI12_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7054907.1"
                     /translation="MMRRALAVTALVCSIGMSSVAVAHDATPTAMGDPDITFVLDEHN
                     HNVTNIDGGVDGPSPGDAIIWGPNPLFDEANENDTGATTQGACTIFAENGDCLLVETI
                     LFPDGSTLELQGIQPGQAIPSTRTIVAGSGMYLGAKGTVTVAPTDDLTVWTKTFEIWL
                     DD"
     gene            898..3357
                     /locus_tag="VFI12_00525"
     CDS             898..3357
                     /locus_tag="VFI12_00525"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET7054908.1"
                     /translation="MSTATTVEYTHCFCALCISRCGAIARTEDGQFVALEPDPAHPTG
                     KALCAKGRAAPELVYHPERLRYPLKRTTPKSDPDPGWERISWGEALDLTATKLRRIAA
                     ERGPESVVFGAVSSSTSAISDALPWIERLRHAFGSPNMCMSMELCGWGRFAAIYTYGI
                     PIGLPGTAMPDLEHAGCMLFWGYNPSSARISHATAAVAALKRGARLIVVDPREVGLAN
                     RADLWLRPRPGSDGALALGIAHAMLANDWFDQEFVRRWTNGPLLVRADSGRLLRTSDL
                     AVDGDPATFVAWDERRGAPIQYDSATRRYADANAQPALDGDDEIETLNGPVRCRPVFA
                     LVREMLSRYDPKTVEAITWVPAERIEAAARMLWDARPVAYFAWAGVEQHTNTTQCSRA
                     LSLLYALTGSFGSRGGNLQFTAVPSAPVAGNELLPPEQRTRTLGLRDRPLGPATAGFV
                     TSEELYRAVLEDEPYPVRGLVGFGTNLLLSHSDTLRGREALRALDFFVHIDLFMTPTA
                     ELADVVLPASTPFEREALKIGFESSAEAQSFVQLRRAIVPPIGEARADTEIVFDLACR
                     LGLGAHFWDGDIDAAYRHQLGPSGISLEALRAYPEGVRMPLETRYRAFADEVDGGVKG
                     FATPTGLAEIYSERFLEYGYLPLPEFEEPLVSQRSRPELAERFPLVLTCTHNPHFCES
                     QHRGIARLRKLSRDPEVELHPDAAADRGIDAGDWVTIETPNGSVRARVRFNGSLDPRV
                     SVGQHGWWQGCAEIDAPSYDPFSADGANLNLIVSSAAVDPVSGSVPHRSYVCEIRGLG
                     PGSRAVLEQPAVSSQQSALDL"
     gene            complement(3428..>4202)
                     /locus_tag="VFI12_00530"
     CDS             complement(3428..>4202)
                     /locus_tag="VFI12_00530"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF009395.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET7054909.1"
                     /translation="PRTTVGWKGLINDPRGDGSYAVNEGLRIARGLLLDVLGLGLPAG
                     CEFLDPISPQFIADAVSWAAIGARTTESQVHRNLSSGLSMPVGFKNGTSGDIQIAIDA
                     IAAASWPHHFMSVTEQGLAAIVQTRGNRDTHVILRGGRSGPNYDGASVESVLQRLRDA
                     KLPPRVMIDASHGNSNKDYSRQPAVASDVAGQIASGQTGVVGLMLESFLVDGRQELDW
                     ANGMTYGQSVTDSCMGWDMTRSVLDDLATAVRARREIRS"
BASE COUNT          675 a         1298 c         1457 g          772 t
ORIGIN      
        1 atttgtgtgc cgcggcacga tcgcgagtca caacctcttc gagtgaggat ggttcacctt
       61 cgccgccccc gacgccctcg tcgacgtggg gacggagacc cccttcaccg cgaccgatgt
      121 ggcgcgtttt tctgcggcgg tggcggtggt ttcccgcagg aatactcgga atgacagcga
      181 ggtgctagga tgcgggaatc gatccgacgt ttcaggaatc ggaggcagtt tcgatgatgc
      241 gccgtgctct ggcagttacc gcgctcgttt gctcgattgg catgagttct gtggccgtgg
      301 cgcacgatgc gaccccgacg gcgatgggcg atccggatat caccttcgtg ctggacgagc
      361 acaaccataa cgtgacgaat atcgatggtg gggtggacgg accgtcacca ggggacgcca
      421 tcatctgggg accgaatcct ctcttcgacg aagcaaacga gaacgacacc ggcgccacca
      481 cccagggggc gtgcacgatc ttcgccgaga acggcgactg cctgttggtc gagacgatcc
      541 tcttcccgga cggcagcacg ctggagctgc aaggaatcca gccggggcag gccatcccct
      601 cgacccgtac catcgtggcc ggaagcggga tgtatctcgg cgcgaagggg acagtgaccg
      661 tggcgccgac cgatgatctg acggtctgga ccaagacctt cgaaatctgg ctcgacgact
      721 gatccggtgc gccgcgtcat tccgggtggt cgatgtccgc ccgggcatgc cgcggcgtcc
      781 atgccctacg atcgtcccag gtcccaggca gctgatgcat ggacaagcga acgggagtag
      841 gatgggccaa cgctcgtcct actcattctg ctcccgtctt gactcaggat gccgccgatg
      901 tccactgcta ccaccgtcga atacacgcat tgcttttgcg cgctctgcat ctcccgctgc
      961 ggggcgattg cccgtacgga agacggacag ttcgtggcgc tcgagccgga tccggcgcat
     1021 ccaacgggga aggcgctctg cgccaagggg cgtgccgcgc ccgagttggt ctatcacccg
     1081 gaacgcctgc gctacccgtt gaagcgaacc actccgaagt ccgatccgga tcccggctgg
     1141 gagcggatca gctggggcga ggcgctcgat ctgaccgcga ccaaactgcg gcgtatcgcg
     1201 gccgagcgcg ggccggaaag tgtggtgttc ggcgccgtct cttcgtcgac ttcggcaatt
     1261 tccgatgcgc ttccctggat cgagcgcctg cggcacgcat tcggctcgcc gaacatgtgc
     1321 atgtcgatgg agctctgcgg gtgggggcgg tttgccgcta tctataccta tgggataccg
     1381 atcggactgc cgggaacggc aatgcccgat ctggagcacg cgggctgcat gctcttttgg
     1441 ggatacaacc ccagtagcgc gcgcatttcc catgccaccg ccgcggtggc tgctctcaaa
     1501 cggggagcgc gtctcattgt ggtcgatccg cgcgaagtgg gacttgccaa ccgggccgat
     1561 ctctggctgc ggccccgccc gggtagcgat ggggcgctgg cgcttggcat cgcccacgcc
     1621 atgctcgcca acgactggtt cgaccaggag ttcgtgcggc gatggaccaa cggcccacta
     1681 ttggtgcgtg ccgattccgg acgattgctg cgcacgagcg accttgcagt ggacggcgac
     1741 cccgcgacgt tcgtcgcgtg ggatgagcgc aggggagctc cgatccagta cgactccgcc
     1801 acccgccggt atgcggatgc aaacgcccag cccgcgctcg atggcgacga cgagatcgaa
     1861 acgctgaacg ggccggtgag gtgccgcccg gtcttcgcgc tcgtgcgcga gatgctgagc
     1921 cgctacgacc ctaagacggt cgaggccatc acctgggtgc cggccgagcg gatcgaggcg
     1981 gcggcgcgga tgctgtggga cgcgcggccc gtcgcctact ttgcctgggc gggggtggag
     2041 cagcatacga acacgacgca atgctcgcgg gcgctgagct tgctctatgc cctgaccggc
     2101 agcttcggca gtcgtggggg caatctccag ttcacggcgg tgccctcggc gccggttgcc
     2161 ggcaacgagc tgctgccgcc cgagcagcgc acccgaactc ttggattgcg ggaccgtccg
     2221 ctgggaccgg ccacagcggg gttcgtgacc tcagaggagc tgtaccgggc ggttctggaa
     2281 gacgagccct atccagtgcg ggggttggtg ggattcggca cgaatctgct gttgtcgcac
     2341 tcggatacgc tgcgcggtcg cgaggcgctg cgggcgctcg atttcttcgt gcatatcgat
     2401 ctcttcatga ccccgacagc agagctggcc gatgtggtgt tgccggcgtc cacaccgttc
     2461 gagcgtgagg cgctcaaaat cggtttcgag tcgagcgccg aggcgcagtc gttcgtccaa
     2521 ttgcggcgcg ccatcgtgcc accgattggg gaagcgcgcg cggatacgga gatcgtgttc
     2581 gatctggcgt gccggttggg attgggcgcg catttctggg atggcgatat cgacgccgct
     2641 tatcggcatc agctcggtcc gtccggcatc tcgctggaag cgctgcgggc gtatcccgaa
     2701 ggcgttcgca tgccactgga gacgcgttat cgcgcattcg cggatgaggt cgatggcggg
     2761 gtgaagggct ttgccacgcc gaccggattg gccgagatct attcggagcg tttcctggag
     2821 tatggatacc tgccgctccc ggaattcgag gaaccgttgg tcagccagcg gtcgcggccg
     2881 gagctggcgg aacggtttcc gctggtgttg acctgcacgc acaacccgca cttttgcgaa
     2941 tcgcagcacc ggggcatcgc acgcttgcgc aagctgtcgc gcgatcccga ggtggaactg
     3001 catccagacg cggcggccga tcgcgggatc gatgcgggcg actgggtgac gatcgaaact
     3061 ccgaacggtt cggtgcgggc gcgcgtccgg ttcaatggat cgctggatcc gcgggtatcg
     3121 gtggggcagc atggctggtg gcaggggtgc gcggagatcg atgcgccgag ctatgacccg
     3181 ttcagtgccg atggcgcgaa tctgaatctg atcgtcagca gcgccgcggt cgaccccgtg
     3241 agcgggtcgg tgccacatcg gtcgtatgtt tgcgagattc ggggcttggg ccctgggtcc
     3301 cgagccgttt tggagcagcc agcggtcagc agtcagcagt cagcactgga cctttgacac
     3361 ttggcgcttg accatcgccg gcacagggcc ggcggctacg ttcagctccg gactccggac
     3421 tccgggttca ggagcgaatc tcgcggcggg cgcggacggc ggtggcgaga tcgtcgagaa
     3481 cgctgcgggt catgtcccag cccatgcagc tgtcagtcac gctctggccg taggtcatgc
     3541 cgttggccca gtcgagctcc tggcggccgt cgacgaggaa gctttcgagc atgagcccga
     3601 cgacgccggt ttgtccggat gcgatctggc cggcgacatc gctggccacg gccggttggc
     3661 ggctgtagtc cttgttgcta ttgccgtggc tggcgtcgat catcacccgc ggcggcagct
     3721 tcgcgtctcg caggcgctgc aataccgatt ccacgctggc gccgtcgtaa ttcgggccgg
     3781 agcggccccc gcgcaggatc acgtgggtat cgcgattgcc gcgggtttgc acgatggcgg
     3841 ccaagccctg ttcggtcacg ctcatgaagt ggtgcggcca ggatgcggcc gcgatggcat
     3901 cgatcgcgat ctggatatcg ccgctggtgc cgttcttgaa tccgaccggc atggagaggc
     3961 cggatgagag attgcggtgc acctggctct cggtggtgcg ggcgccaatg gcggcccagc
     4021 tcaccgcatc ggcgatgaac tggggtgaga tcgggtcgag gaactcgcac ccggcgggca
     4081 ggccgagtcc cagcacgtcg agcagcagac cgcgagcgat gcgcaatccc tcattgacgg
     4141 catagctacc gtcgccgcgc ggatcgttga tcaagccctt ccagccgacg gtcgtgcgcg
     4201 gc
//
LOCUS       DASSGX010000034         2004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1093204, whole genome shotgun sequence.
ACCESSION   DASSGX010000034 DASSGX010000000
VERSION     DASSGX010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1093204"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..1466)
                     /locus_tag="VFI12_00535"
     CDS             complement(42..1466)
                     /locus_tag="VFI12_00535"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7054910.1"
                     /translation="MTRAHDAVIIGSGPNGLAAGVTLAQAGRSVLILEAREQIGGGSR
                     TQELTLPGFRHDTCSAIHPLAVASPFFRSLPLADYGLEMIQPPLALAHPFDDGTAAVL
                     ARSVDETVASLGSDANAYRKLFDPLIRDWPILANELLGPLRIPPRHPIALARFGLVGL
                     RSARGLAMSRFEGRNAPALFAGMAAHSMLKLDQLTTAAFGLTLALFGHAVGWPIPRGG
                     SQAIADALAGHFRMLGGEILTGQLVESITGFDDANTILFDVTPRQFLSLAGDRVPGRY
                     RRQLRHYRYGPGVFKMDWALDGPVPWTAPECRRAGTMHLGGTFEEIAAGERDANEGRH
                     SERPLVLVAQPSLFDPTRAPEGKHTLWAYCHVPSGSDQDMTGAIEAQIERFAPGFREL
                     ILARSVTGPSALERDNPNYVGGDINGGVQDIRQLYTRPAVRLDPYSTPNKRLFICSSS
                     TPPGGGVHGMCGYHAARSVLRRET"
     gene            complement(1463..>2004)
                     /locus_tag="VFI12_00540"
     CDS             complement(1463..>2004)
                     /locus_tag="VFI12_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7054911.1"
                     /translation="WKQHFGELWPGKNRFFGPITALEPGELAVISIEMPADTTLSTGV
                     ILVDATPTGFTLITPKGHMLCGWLQFSAFAGGGATVAQVEILIRASDPLFEIGMVLGG
                     HRRENHFWEQTLRNLAAHFGVRAEPETSVTCEDPQYKWENAKNIWHNGAIRNGLIRLA
                     AIPRRAVDLLSRRGAERTP"
BASE COUNT          332 a          619 c          742 g          311 t
ORIGIN      
        1 ggcaatatct agtcttggtc cgtgtctcct caatcctcaa ctcacgtctc acgtctcaag
       61 actgaccgcg cggcgtgata gccgcacatg ccgtggacac cgccgccggg cggggtggac
      121 gaggagcaga tgaagaggcg cttgttgggc gtcgaatagg ggtcgaggcg gacagccggg
      181 cgggtgtaga gctggcggat gtcctggacg ccgccgttga tgtcgccgcc gacgtagttc
      241 gggttgtccc tttccaacgc gctcggcccc gtcacggagc gggcgaggat cagctcccgg
      301 aagccggggg cgaagcgctc gatctgggcc tcgatcgcgc cggtcatgtc ctggtcggag
      361 ccgctgggga cgtggcagta ggcccagagg gtgtgtttgc cctccggagc gcgagtcggg
      421 tcgaagaggc tcggctgggc gacgaggacg agcggccgct ccgagtggcg cccttcgttg
      481 gcatcgcgtt caccggcggc gatctcctcg aacgtcccgc cgaggtgcat ggtcccggcg
      541 cggcggcatt cgggcgcggt ccacggtact gggccatcga gggcccaatc catcttgaag
      601 acgccgggtc cgtaccgata atgccgtaac tggcgccggt agcggccggg aacgcggtca
      661 ccggcgaggg aaaggaactg gcgcggcgtg acgtcgaaca ggatcgtgtt cgcatcatcg
      721 aagccggtaa ttgattcgac caactggccg gtcaggatct cgccgccaag catgcggaag
      781 tgcccggcga gggcgtcggc gatggcctgc gagccgccgc gcgggatcgg ccagccgacg
      841 gcgtggccga aaagcgcgag tgtcaggcca aaggcggcgg tcgtgagctg gtcgagcttg
      901 agcatcgagt gggcggccat gccggcgaag agggcaggcg cattccgccc ctcgaagcgg
      961 gacatcgcca gtcccctggc cgaacgcaat ccgaccaggc cgaagcgggc gagcgcaatc
     1021 gggtggcgcg gcggaatccg aagcgggccg agcagctcat tggccaggat cggccagtcc
     1081 ctgatcagcg gatcgaagag cttccggtac gcgttcgcat cagagccgag cgaggcgacc
     1141 gtctcatcca ccgagcgggc aagcacggcg gccgtgccgt cgtcgaatgg atgcgccagc
     1201 gcgagcggcg gctggatcat ctccaggccg taatcggcga gggggagcga ccggaagaag
     1261 ggtgacgcaa cggccagcgg atgaatcgcc gagcaggtgt catggcgaaa accggggagc
     1321 gtgagctcct gggttcggct gccgccgccg atctgctccc gggcctcgag aatcaggacg
     1381 gagcggccgg cttgggcgag ggtgacgccg gcggccagcc catttggccc ggagccgatg
     1441 atgacggcgt cgtgcgccct ggtcacggtg tcctttctgc cccgcggcgg gaaagcagat
     1501 cgacggccct gcgcgggatt gccgccagcc ggatcagccc gttccgaatc gccccattgt
     1561 gccagatgtt tttcgcgttc tcccacttat attgggggtc ctcgcaggtg acgctggttt
     1621 ccggctcggc gcggacgccg aaatgcgcgg cgaggttgcg cagggtctgt tcccagaagt
     1681 gattctcgcg gcggtggccg cccaggacca tcccaatctc aaacagggga tcgctggcgc
     1741 ggatcaggat ctcgacctgc gccaccgtgg cgccgccccc agcgaaggcc gagaactgaa
     1801 gccagccgca gagcatgtgg cccttcggcg taatcagggt gaagccggtc ggggtggcgt
     1861 cgacgaggat cacgccggtc gagagggtgg tatcggcggg catctcgatg ctgatcacgg
     1921 ccagctcgcc cggctcgagc gcggtgatcg ggccgaagaa gcggttcttt cccggccaga
     1981 gctcgccgaa gtgctgcttc cagg
//
LOCUS       DASSGX010000035         2107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_984688, whole genome shotgun sequence.
ACCESSION   DASSGX010000035 DASSGX010000000
VERSION     DASSGX010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_984688"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(222..1067)
                     /locus_tag="VFI12_00545"
     CDS             complement(222..1067)
                     /locus_tag="VFI12_00545"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET7054912.1"
                     /translation="MRLRLSLAAVAIALGSLAYYRLRHVNPYRPRLERIEIPLPPGAG
                     GLDGLRIGFVTDTHVGPFTSANDLRRGLALFDDEPLDLLLLGGDYVSESNLYAPAMAD
                     AIGSLAARAHHGAFAVMGNHDLPLGVDRVRAELERVGVRVLRNEHAIVEWHGAQLAVV
                     GIDDTVVGQADPNCAFAGIPARVPRLSLWHEADFAEQAAQHGAFAQLSGHTHGGQVRL
                     PLIGAIWLPPDGKRRDMGCFDVEGMTLYVARGLGVYRPPVRLCCPPEVTLVTLRSSPA
                     QPAMR"
     gene            complement(1071..1994)
                     /gene="menA"
                     /locus_tag="VFI12_00550"
     CDS             complement(1071..1994)
                     /gene="menA"
                     /locus_tag="VFI12_00550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HET7054913.1"
                     /translation="MSAPALPAPGSLGAWLRAVRYHSFTASIIPILTGSALALIDRQF
                     SWPLVIAMLLAAVACHAGANMANDYFDFRKGVDNERNLGPHKVIQLGLLTANQVRNGF
                     SAAFALATLIGVYIVLETDWKIFVLAFVSLGAAFFYTAGSKALGYVALGEVTVFIFMG
                     PVMVCGAYYVLTGAVSWASFLLSIPIGAISAATLHANNMRDIEHDRAAGKITLATILG
                     RRRAATEYLLWIVVSYLAVLLTVLVDRVLWPVLLVLVTVLTAIRLIELARTGADAASL
                     NRLLRKTAGLHLRFGSLLVAGLLIRAALDRL"
BASE COUNT          430 a          640 c          720 g          317 t
ORIGIN      
        1 gaccagagat ggcaacgacc ctggcgcggg aatcggtatc gtatccctcc agatgccatt
       61 tgccgattcc gcaaccgatg acgccgatac cgacggtttc cattgtgttg tgcgttcctc
      121 aaaagcgaag gccggattca atgcgccatc ctggcttcat tgccgggagg tctgccgcga
      181 agtttagagg gatcgcgtct cgcatctcac cgggcatcgc gtcatcgcat cgccggttgc
      241 gccgggctgg agcgaagcgt tacgagggtc acctccggag ggcagcataa tcggaccggc
      301 ggccggtaga ccccgagacc tcgggcgacg tagagcgtca tcccctcgac atcgaagcag
      361 cccatatcgc gccgcttgcc atcgggtggc agccagatgg cgccgatcag cgggagccgg
      421 acctggccac cgtgggtgtg cccggagagc tgggcaaacg ccccatgctg agcggcttgc
      481 tcagcgaaat cggcttcgtg ccagagcgaa agcctgggca cgcgggcggg gatgccggcg
      541 aacgcacagt tcggatcggc ctggccgaca accgtgtcgt cgattcccac gactgccagc
      601 tgagcgccat gccattcgac gatggcgtgt tcgttgcgca gcacccgcac gccaactcgt
      661 tcgagctcgg cgcgcactcg gtcgacaccg agcggcaggt cgtgattgcc cataaccgcg
      721 aaggcgccat ggtgtgcgcg agctgccaga gagccgatcg catcggccat ggcgggggca
      781 tagagattcg attcggagac gtagtcgccg ccgagcagca ggagatcgag tggttcgtcg
      841 tcgaagagcg ccaatccccg acggagatcg ttggccgagg tgaagggacc cacgtgggta
      901 tcggtgacga agccgatgcg caggccatcg agccctccgg cgcccggtgg gaggggaatc
      961 tcgatgcgct ccaaccgcgg acgataggga ttcacatgac ggagccggta ataggcgagc
     1021 gatccaagcg cgattgcgac cgcggcgagc gagagacgga ggcgcatcgg ctagagccgg
     1081 tcgagcgcgg cgcggatcaa caatccggcg accagcagac tgccgaatcg aaggtgcaat
     1141 cccgcggtct tgcgcagtag ccggttgaga ctggccgcat cggcgcccgt gcgggccagc
     1201 tcgatcagcc ggatcgcggt gaggaccgtc acgagcacca gcagcaccgg ccagaggacg
     1261 cggtcgacca ggacggtcag caacacagcg agataggaca ccacgatcca gagcaggtat
     1321 tcggtggccg cgcgccggcg tccgaggatg gtcgcgaggg tgatcttgcc ggcggcgcgg
     1381 tcgtgctcga tatcacgcat gttgttggca tgaagcgtcg ccgcggagat ggcgccgatc
     1441 gggatcgaga gcaggaacga tgcccaactc actgcgccag tcaggacata gtaggcgccg
     1501 cagaccatga ccggtcccat gaagatgaag accgtcactt cgccgagcgc gacatagccc
     1561 aacgccttcg agccggcagt atagaaaaat gccgcaccga ggctgacgaa cgccaacacg
     1621 aagattttcc aatcggtctc caggacgatg tagacgccga tgagcgttgc cagcgcgaag
     1681 gccgcgctga acccattgcg gacctgatta gcggtgagca atccgagctg gatgactttg
     1741 tgcggtccga ggttgcgctc gttgtcgacg ccttttcgga aatcgaaata gtcgttggcc
     1801 atattggcgc cggcgtgaca ggcgaccgcg gcgagcagca tggcaatgac cagcggccag
     1861 gagaactgcc ggtcgatgag ggcgagtgcg gatccggtga ggatgggaat gatcgaggcg
     1921 gtgaacgagt ggtagcggac cgcccgcagc caggcgccca gcgagccagg cgcgggcaac
     1981 gcgggcgcgc tcatcgcgcc tgacgccatg gcaccagcag ccgttcgatc agatcgagca
     2041 ccagaaccag cacccagccg aggacgccgg tgacgaccag cccgacatac atcttcttga
     2101 tcgagag
//
LOCUS       DASSGX010000036         2109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_876547, whole genome shotgun sequence.
ACCESSION   DASSGX010000036 DASSGX010000000
VERSION     DASSGX010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876547"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1864)
                     /locus_tag="VFI12_00555"
     CDS             complement(<1..1864)
                     /locus_tag="VFI12_00555"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET7054914.1"
                     /translation="MTLDIRSNSDVAWTPTQAYRDRSRLLALMSQHGVETVDDWRRWA
                     AEDIGRYWDTVVRDLCLDFSAPYEQVVDLGNGAPWAEWFVGGSFNYVANALDRQAAGP
                     MRDKLALIWEGDDGSQRTVNFAELLAETNQVANALKGLGISKGDIVGIFMPMAPETVS
                     AVLACGKIGAIYTPLFSGFGAEAVASRLRDCDARLLITADGFFRRGSTIAMKPVADEA
                     AETSPAVEHVLVLRRAGNDVAMAPGRDIWWHDVVPAASTECETLETKANDPYMVIHTS
                     GTTGRPKGAVHVHAGFPIKAAHDLAYCFDLHEDDVLFWYTDLGWMMGPWAIGGGLMLG
                     ATLMLFEGTPDYPHPDRIWEIVERHGVTILGIAPTAIRALIPKGDEWPARHDLSSLRV
                     LGSTGETWNPDPWRWYFDKIGGGKLPIVNYSGGTETGGGIVGCDTISPIKSCCFTGPV
                     VGMDADVVDDQGNPVRCEVGELVIRQPWVGMTRGFWRDPERYLDAYWSRMPGLWVHGD
                     WAEIDEDGFWFIRGRSDDTLKVAGKRVGPAEVESAAVAHNNVQEAAAIGIPHAVKGEA
                     VVVLCVLKGAPGASAQLAEDIRQTVSHHLGSALRPESVHFVTELPRTRNAKIMRR"
BASE COUNT          376 a          725 c          643 g          365 t
ORIGIN      
        1 cccggcgcat gatcttggcg ttgcgggtgc gcggcagttc cgtaacgaag tggaccgatt
       61 ccggccgaag cgcagaaccg aggtggtgac tgacggtttg ccggatatcc tccgcgagtt
      121 gggcggatgc gccgggtgcg cctttcagta cgcagagcac gacgaccgcc tcgcctttta
      181 ccgcatgcgg aatcccgatg gcggccgctt cttgcacgtt gttgtgcgca acagcagccg
      241 attcgacctc ggccggtccc acccgcttgc cggcaacctt gagcgtatcg tctgaccggc
      301 cccggatgaa ccagaacccg tcttcgtcga tctcggccca gtcgccgtgc acccacagcc
      361 ccggcattcg cgaccaatag gcatcgagat aacgctcggg atcgcgccag aatccacgcg
      421 tcatcccgac ccacggctgg cgaatgacca gctctccgac ctcacaccgg accgggttcc
      481 cctggtcgtc aacgacgtcg gcgtccatgc cgaccaccgg gcctgtgaag cagcacgact
      541 tgatcggcga gatggtgtcg cacccgacga tgccgccgcc tgtttcagtg ccacccgagt
      601 aattgacaat cggcagcttc ccaccgccga tcttgtcgaa gtaccagcgc catgggtccg
      661 gattccaggt ctcgccggtt gagccaagca cccgaagcga cgagagatcg tgccgggcgg
      721 gccactcgtc acctttcggg ataagcgcgc ggatcgcggt cggcgcgata ccgagaatcg
      781 tcacgccgtg ccgctcgacg atttcccaga tccggtccgg gtgcgggtag tccggcgtgc
      841 cttcgaacag catcagcgtc gcgccgagca tcaacccgcc gccgatcgcc catggcccca
      901 tcatccagcc gagatccgta taccaaaaga ggacgtcgtc ctcgtgcaga tcgaagcagt
      961 aggcaaggtc gtgtgcggcc ttgattggga atccggcgtg gacatggacc gcgcccttcg
     1021 gccggcccgt cgtccccgag gtgtggatga ccatgtatgg atcgttcgcc ttggtctcga
     1081 gcgtctcgca ctcggtcgag gcggccggga cgacatcatg ccaccagatg tcccgacccg
     1141 gcgccattgc gacgtcattg ccagcgcgcc ggagcacaag cacgtgctca accgccggcg
     1201 acgtctccgc cgcctcatcg gcgaccggct tcatggcgat cgtgctgccg cgccggaaga
     1261 agccatcggc ggtgatcagc aacctggcgt cgcaatccct caaacgggac gcgaccgcct
     1321 ccgccccgaa tcccgaaaag agcggcgtgt agatcgcccc gatcttcccg caggcgagaa
     1381 cggcggatac cgtctccggc gccatcggca tgaagatccc gacgatgtcg cccttgctga
     1441 ttccaagccc cttgagcgcg tttgcgacct ggttggtctc cgcgagtagc tcggcgaagt
     1501 tcacggtgcg ctgtgagccg tcatcgcctt cccagatcag ggccagcttg tcccgcatcg
     1561 gccccgccgc ctgtcggtcg agcgcgttgg cgacgtaatt gaacgacccg ccgacgaacc
     1621 actccgccca cggcgcaccg ttgccgagat cgacgacctg ctcatacggg gccgaaaaat
     1681 cgaggcagag atcccgcacg accgtgtccc agtatcggcc gatatcctcg gccgcccacc
     1741 ggcgccagtc gtcgactgtc tcgactccgt gctgtgacat cagcgcgagc agcctgctcc
     1801 ggtcgcggta ggcttgtgtc ggcgtccacg cgacgtcgga gttcgatcga atatcgagcg
     1861 tcatgtcctg aggatccccc gcggtgcgcc gcatgtccat cgtgcctgca atactgactg
     1921 ggacggggaa tgtggtcaag gccgtttggc gtgtccgaat aacgtatcaa ggagcagcag
     1981 gtggaccgac tgaaattctg gaagcggaac cagccggagc agccatcggt cgagccgatc
     2041 ccgaccatca agcggttcaa tatccagccg cggaccgaca tcggcacgct cggcctcccg
     2101 tctgatccg
//
LOCUS       DASSGX010000037         2394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_876715, whole genome shotgun sequence.
ACCESSION   DASSGX010000037 DASSGX010000000
VERSION     DASSGX010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876715"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="VFI12_00560"
     CDS             complement(<1..587)
                     /locus_tag="VFI12_00560"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HET7054915.1"
                     /translation="MQSKITTLAEAVARIPDGGRLALGGNTIHRGPGAAVHEIVRQGK
                     RGLDVIKTAGAYDIDLLCATGCATSVSAGYIGYETVLGLAPSYRAAVESGAVEAREHA
                     CYTVISGLRAAIQGVPFMPVAGLLGSDLLAARDFRTVTDPYTGEEVVAIQAIRPDVAI
                     IHVQEADERGNARIVGTHFEDVLMAQAADVVILTA"
     gene            678..2285
                     /locus_tag="VFI12_00565"
     CDS             678..2285
                     /locus_tag="VFI12_00565"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HET7054916.1"
                     /translation="MYPPEVYKSYLTPLRFLQRSADVFREKVAIIHRDQRITYPEFAT
                     RVNRLASALRAAGIEHGDRVAVLLPNIPQMLEAHFGVPLSGGIMVAINTRLKTDEIAY
                     ILNHSSAKALIVDSELAPLVEPVLRDIPALESMVTVHDIDAGIHLDGPDYEAFLATGS
                     PEPLDWPIDDEDQVVSINYTSGTTGKPKGVMYTHRGAYLNSLGELLETRMSSTSNYLW
                     TLPMFHCNGWCYPWAVTAIGGTHVCLRRFDPGEAWDLIRTHTVTHLCGAPTVLISLAN
                     HLSAPTEPLPHRIIFSVAAAPPSPAIIAQMESLGIEIVHVYGLTEVYGPYTVCEWQEQ
                     WDALPLDERSRTKARQGVGYLVADDVRVVDDNMIDVPADGLTIGEVIMRGNNTMKGYF
                     NDDEATERAFRGGWFHSGDLGVMHPDGYLELRDRAKDIIISGGENISTIEVEQVIAAH
                     PAVLEVAVIGIPDDYWGEIPKAFVVLREGATATEQEIIDHCKQRLAGFKCPKAVQFGE
                     LPKTSTGKVQKFALREKEWAGHAGRVH"
BASE COUNT          431 a          825 c          745 g          393 t
ORIGIN      
        1 tcggccgtca gaatcacgac gtcggccgcc tgcgccatca gcacgtcctc gaagtgcgtg
       61 ccgacgatcc gggcattgcc acgctcgtcc gcctcctgca cgtggatgat cgccacgtcg
      121 ggccggatcg cctggatcgc gacgacctcc tcgccggtgt acggatcggt cacggtccga
      181 aaatcgcgtg ccgcgagcag atcggatccg agcagccccg cgaccggcat gaaggggacg
      241 ccctggatcg ccgcgcgcaa cccgctgata accgtgtaac aggcatgctc ccgagcctcg
      301 accgcgccag attcgaccgc cgcgcggtag gatggggcca gcccgaggac cgtctcgtac
      361 ccgatgtagc cggccgaaac cgatgtcgcg cagcccgttg cgcagagcag gtcgatgtcg
      421 tatgcgccgg ccgtcttgat cacgtccaat ccgcgcttcc cctgccggac gatctcgtgc
      481 accgccgcgc cgggtccccg gtggatcgtg ttgccgccga gcgcgagccg tcccccatca
      541 ggaatccggg cgacggcctc ggccagcgtc gtgatcttcg attgcatggc gaacctcccg
      601 cgcgtgccgt gcgcgtcagc aactatcata gcgggaccta tcgtcaccgg cccgccagac
      661 gtttcgggaa ccctgccatg tatccaccag aggtttacaa gtcctatttg accccgctcc
      721 ggttcctgca acgctcggcg gatgtcttcc gcgagaaggt cgcgatcatc caccgcgacc
      781 agcgaatcac ctacccggaa ttcgccacgc gggtcaaccg gctggcgtcg gccttgcgcg
      841 cggccggaat cgagcatggc gaccgcgtcg ccgtactgtt accgaacatc ccgcaaatgc
      901 tcgaggcgca cttcggcgtt ccgctctcgg gcgggatcat ggtcgcgatc aatacgcgcc
      961 tcaagaccga cgaaatcgcc tatatcctca accactccag cgcgaaggcg ttgatcgtcg
     1021 acagcgagct cgctccgctc gtcgaaccgg tgctccgcga catcccggca ctggaatcga
     1081 tggtgacggt ccacgacatc gacgccggga ttcacctgga cggtcccgac tacgaggcct
     1141 tcctcgcgac cggctcgccg gagccgctcg attggccgat cgacgacgag gatcaggtcg
     1201 tctcgatcaa ctacacgagc gggaccaccg gcaagccgaa aggcgtcatg tacacccatc
     1261 gcggcgcgta cctgaactcg ctcggcgagc tgctcgaaac gcggatgtcc agcacgagca
     1321 attacctttg gacactgccg atgtttcact gcaacggttg gtgttacccg tgggctgtga
     1381 ccgcgatcgg cggcacccac gtctgcctgc gcagattcga cccgggcgag gcgtgggacc
     1441 tgatccgcac gcacaccgtg acgcacctgt gcggcgcgcc gaccgtgctg atctcgctcg
     1501 ccaatcatct gtcagccccg acggagccac taccgcaccg gatcatcttc tccgtcgccg
     1561 ccgcgccgcc gtcgcccgcg atcatcgccc aaatggagtc gctcgggatt gagatcgtcc
     1621 acgtctatgg cctgaccgag gtctacggcc catacaccgt ctgtgagtgg caggagcaat
     1681 gggatgcgct gccgctcgat gagcggagcc gcaccaaggc gcggcaaggg gtcggttacc
     1741 tggtcgccga cgatgtccgt gtcgtggacg acaacatgat cgacgtgcct gcggacggcc
     1801 tgacaattgg cgaagtgatc atgcgcggca acaacacgat gaaaggctat ttcaacgacg
     1861 acgaggcaac cgagcgcgcc ttccgcggcg gctggttcca ctccggcgac cttggcgtga
     1921 tgcaccccga cggctacctc gagctgcggg accgggccaa ggacatcatc atcagcggcg
     1981 gcgagaacat ctcgacgatc gaggtcgagc aggtgatcgc cgcccatccg gccgtcctcg
     2041 aagtcgcggt gatcggcatc ccggacgact actggggcga gatcccgaag gcattcgtcg
     2101 tcctgcggga gggcgcaacc gcgaccgagc aggagatcat cgaccactgc aagcagcggc
     2161 tggccggctt caaatgcccg aaggcggtcc aattcggcga gttgccgaag acttcgaccg
     2221 gcaaggtcca gaagttcgcg ctgcgcgaga aggaatgggc cgggcacgcc ggccgggtac
     2281 actagcgcgc gacacgggaa ctactgcatg cgacaccttc acattctctc ggttctgatg
     2341 ctcctgatcg gcgtggcagt caccgcggcc ggcgtcgcgc tcgagcttgg cgcg
//
LOCUS       DASSGX010000038         5399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_223604, whole genome shotgun sequence.
ACCESSION   DASSGX010000038 DASSGX010000000
VERSION     DASSGX010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5399
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223604"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..898
                     /gene="lepB"
                     /locus_tag="VFI12_00570"
     CDS             221..898
                     /gene="lepB"
                     /locus_tag="VFI12_00570"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET7054917.1"
                     /translation="MAEPHEQQSQAAPEGSEISRHSLWSELFKTVMMALIIFLTARLL
                     ILPYQVDGRSMAPNLENRDRVLVNRAVYMHLNLEALIGWIPGIDTSDTDYYPFHSPER
                     GDIVVVYPPQYSSEPYIKRTIAVAGDVVDIHDGLVYVNGQVLTEPYIDGAITDCEQPT
                     YCRGYVVPDGKIFVLGDNRQHSYDSRAFGPVSLSNVIGKAWFANWPADRFGPIPHYDY
                     DDKNARQ"
     gene            complement(985..1758)
                     /locus_tag="VFI12_00575"
     CDS             complement(985..1758)
                     /locus_tag="VFI12_00575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7054918.1"
                     /translation="MLGTVLTGKVAIVTGAGSGLGQATAIALAAEGAHVCVTELPDRI
                     DRAGETLSTIIHAGGHGHVRELDVRSLNSIDECVSSLIGETSRLDILVNNAGINIRKP
                     ALEVTETDWDTVLDVNLKGAFFMAQAAGRQMVSQNPLGGAIINISSIMGLGGYWDRVA
                     YCASKAALINMGRVLALEWGPHHVRVNAVCPNFVITPLTEPLFAANPEFTEDVMRRTP
                     IGRLPTPAEIAAAVVYLASPAAASVTGQALAVDGGWTAH"
     gene            2131..3222
                     /locus_tag="VFI12_00580"
     CDS             2131..3222
                     /locus_tag="VFI12_00580"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET7054919.1"
                     /translation="MGQLGKGLNRKEVRSLVENITRRGFLGGGASFTALAAMGGLRPG
                     VTAAKALNNLGILPDDLPAPKYKAATIEVGAASTWVAHGIETSKFFGKLLGVEVEPFD
                     GQFDPANQLSAFQTVSQGTYDFVCVHPAASDALIDGSDAVIAKGWPLIVMDTRLIQDP
                     DAFSKYGHLTFLEPDNVYMGFTVANELFKAIGGEGEVIHTQGQLAHTGAQGRAQGFKQ
                     ALEAYPNIKVVDEQPGDWQVEKVATLWQDLLQRFPNVKGGFFHSDDMALAARAVIDAA
                     GKRDQVKIVGVDGLKNACEAIIKGDMTASVINPSGRDHGGAIWAGYLTVSKTDMSTAP
                     IPKFIRMDGGPITIDNAPGYIWLGDNMQY"
     gene            3264..4883
                     /locus_tag="VFI12_00585"
     CDS             3264..4883
                     /locus_tag="VFI12_00585"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HET7054920.1"
                     /translation="MAEAPGTLNIEQDVSSSSLSSPTSGLLPGEGEVSPSPGPSPDDE
                     SVVLELRHVVKRFGGAVALSGVDFTLRRGEIHGLLGENGAGKSTLMKILSGVHSPDEG
                     EIILEGQPVKFHSPAGAKALGIGMIYQELTTMPSLTVAENVFLGRQYTNRFGLIDWGR
                     MRTDAAGALHALGIDIDITQRMGQLSLGNQQLVEIARIILSGAKIIVLDEPTSALSGP
                     ESERLFGLMRQLKARGTSMIFISHFIEDVMAVSDRITILKNSRKVAELENTGLTKRKL
                     VELMIGRDATELAESYEGGTQLPPRVDGETALRVESLSVPGEFHDITFDVKHGEILGM
                     FGYLGGGMTEVARALFGRLKPSRGTIVLDGEVVRPTTPIKAKRLGIAYLTENRRATLF
                     PRHEIYKNITLAHLGHLVPPLFSARNEMAVADEHIRLTGVRPANSRMRAGHLSGGNQQ
                     KVVLAKWLTRRPKVLILNEPTRGMDVGAKREVLDLIRTLKAQGVAIILMSTEPETVMA
                     ESDRIIVMSKGKITAEFADQEVTKEQLLVHA"
     gene            5039..>5399
                     /locus_tag="VFI12_00590"
     CDS             5039..>5399
                     /locus_tag="VFI12_00590"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7054921.1"
                     /translation="MAPLITLIVEVIFFSFASDVFLTRSNFQNVVSQVGPVAVAATGI
                     TFVLLCAEIDLSIASISVFCGIVATYIFSNDWHGLGQWGILVAAAAGAAIGLINGFFI
                     CYVGIPSFMMTLAMLTIA"
BASE COUNT         1021 a         1633 c         1757 g          988 t
ORIGIN      
        1 gaattcggtc ccggtttgtg cgcggagacc ttgccgggaa cgggtcagat gggcaagata
       61 ggggaatgtt tttgctgccc gaaagggcca ttctgccgat ctggccccat cctgccaagt
      121 ggcgaggcga cgctccggga atccgggctg gtcgcgtggt cgttcagaaa gtgcaggatg
      181 gcgcatcgcc ggcggcccgt aaccggagag tttgtcgcta gtggctgaac cccacgaaca
      241 gcagagtcag gcagcccccg aggggagcga gatctcgcgc cactcgttgt ggtcggagct
      301 gttcaagacc gtgatgatgg cgctgatcat cttcctgacg gcgcgcttgc tgatcctgcc
      361 gtaccaggtc gatggccgga gcatggcccc gaacctcgaa aaccgggacc gggtgctggt
      421 caaccgggcg gtctacatgc acctcaacct ggaggcgctg atcggctgga ttccgggcat
      481 tgacaccagt gacaccgact attacccgtt tcactcgccg gagcggggcg acatcgtcgt
      541 ggtctatccc ccgcagtact catcggagcc ttacatcaag cggacgatcg cggtcgccgg
      601 agacgtggtc gacattcatg acgggctggt ctacgtcaac ggccaggtgc tcaccgagcc
      661 gtacattgac ggcgcgatca ccgattgcga gcaaccgacc tattgccgcg ggtacgtcgt
      721 cccggacggg aagattttcg tcctcgggga caatcggcaa cacagctatg attcccgcgc
      781 cttcggtccg gtgtcgctca gcaacgtgat cggcaaagcc tggttcgcca attggccggc
      841 ggaccgattc ggccccatcc ctcactacga ctacgacgac aagaacgcca ggcaatagca
      901 gagccgtctt gacgtgttgc tcagcccggg catttgacgc cgggcggcgt tggcacgtcc
      961 ctgggggtgg gacctccgcc ttgcctagtg ggcggtccag ccgccgtcga cggccagggc
     1021 ttggccggtg actgaggcgg cggcggggct ggcaaggtag acgacagcgg ccgcgatctc
     1081 cgcgggcgtc ggcaggcggc cgatcggcgt ccggcgcatg acgtcctcgg tgaattccgg
     1141 gttggcggcg aagagcggct ccgtcagcgg cgtgatcacg aagttcggac agacggcgtt
     1201 gacccggaca tggtgcgggc cccattcgag cgcgaggacc cggcccatgt tgatcagggc
     1261 ggccttgctc gcgcagtagg cgacgcggtc ccaatagccg ccgagtccca tgatgctcga
     1321 gatattgatg atcgctccgc ccaaggggtt ctggctgacc atctggcgac cggcggcttg
     1381 tgccatgaaa aacgcgccct tcaggttgac gtcgaggacc gtgtcccagt ccgtttccgt
     1441 gacctcgagg gctggtttgc ggatgttgat gcccgcgtta ttgaccagaa tgtcgagtcg
     1501 cgacgtctcc ccaatcaggc tgctgacgca ctcatcgatg gagttgagtg agcgcacgtc
     1561 gagctcgcgg acgtgcccat gtccaccggc atggatgatc gtcgagagcg tttcgccggc
     1621 gcggtcgatc cgatcgggca gctccgtgac acagacgtga gctccctcgg cggcaagcgc
     1681 gatcgccgtc gcttgcccca gcccggagcc cgccccggtc acaatcgcca ccttccccgt
     1741 caatacggtc cccaacacgc ttcctcccct gttcacccgg gattgtatga ggcgaggcgg
     1801 cgagcaggcg aggagcgagt agccactagc cagtagtcgg tagccagttc ggacaacgaa
     1861 gaacggaaaa cggaaagcga gagacgaaag accagagacc aaaaaccaaa gacccggccc
     1921 gcgtcctgtt gaccggttcc cgggcatatg ctagtttgcc taacgtttcg acaagatagg
     1981 gcgcgaggag atcagggccg gaggcctcga cctcatgttc accaaccttg ggccacgtga
     2041 atgccgcatc tgccggcatt cccgggtaat aacgacaatc gcggcgtggt gccgcggttg
     2101 tggaccaata accgacggag gaagatcggg atgggccagc tgggaaaggg actgaaccgc
     2161 aaagaggttc gctcactcgt agagaacatc acgcgccggg gcttcctcgg tggtggtgca
     2221 tcattcacag cgctggcggc tatgggcggt ctgcgcccgg gcgtgacagc ggcgaaggcg
     2281 ctcaacaatc tcggcatcct gccggacgat ctcccggcgc cgaagtacaa ggcagctacc
     2341 atcgaggtcg gcgcggcctc cacatgggtc gcacacggca tcgagacgtc gaagttcttc
     2401 ggcaagttgc tcggcgtcga ggtggagcca ttcgatggtc agttcgaccc ggccaaccag
     2461 ctctcggcct tccaaacggt gtcgcagggt acctacgact tcgtgtgcgt ccacccggca
     2521 gcctcggatg cgttgattga tggctccgac gcagtcatcg ccaagggctg gccactgatc
     2581 gtcatggata cgcgcctgat ccaggacccg gacgcgttca gcaagtacgg ccacctgaca
     2641 ttccttgagc cggacaacgt ctacatgggc ttcacggtcg ccaatgagct cttcaaggcg
     2701 atcggcggcg aaggcgaggt gatccacacc cagggtcagc tcgcacacac cggcgcccag
     2761 ggccgcgctc agggattcaa gcaggcgctc gaggcctatc cgaatatcaa ggtcgtcgat
     2821 gagcagccgg gcgactggca ggtcgagaag gtggccacgc tctggcagga cctcctccag
     2881 cgcttcccga atgtcaaggg cggcttcttc cactcggacg acatggcgct ggcggctcgg
     2941 gcggttatcg acgcggctgg caagcgtgac caggtcaaga tcgtgggcgt ggacgggctg
     3001 aagaatgcct gcgaggcgat catcaagggt gacatgaccg cttcggtcat caacccgtcg
     3061 ggccgcgatc acgggggagc catctgggcc ggatacctga ctgtatccaa gacggacatg
     3121 tcgaccgcgc cgattccgaa gttcattcgc atggacggtg gtccgatcac gatcgataac
     3181 gcgcccgggt acatctggct cggcgacaac atgcagtact agaaggcccc gatcgagggc
     3241 ggcgaagaca agggaacgga ttgatggcgg aagcacccgg aacgctcaac atcgagcagg
     3301 atgtgtcgtc ctcctccctc tcttcaccga cctctggcct tctgccgggc gagggcgaag
     3361 tatcgccttc gcccggtcct tctcccgacg acgagtcggt cgtcctcgag ttgcgccatg
     3421 tcgtaaagcg attcggcggt gcagtcgcgc tcagcggtgt cgatttcacg ttgaggcgcg
     3481 gcgagattca cggcttgctt ggtgagaacg gcgccggcaa gagcacgctc atgaagatcc
     3541 tgagcggcgt gcactcgccg gatgagggcg agatcatcct cgagggccag cccgtcaagt
     3601 tccattcccc ggccggggcg aaggcgctcg gcatcgggat gatctaccag gaactgacga
     3661 cgatgccatc gctgacggtc gctgaaaacg tctttctggg tcgtcagtac acgaaccggt
     3721 tcggcctgat cgattggggc cgcatgcgca ctgatgcagc cggagcattg catgccctcg
     3781 gcatcgatat cgacatcacc cagcgcatgg gccagctttc gctgggaaat cagcagttgg
     3841 tcgaaatcgc ccggatcatt ctttcggggg ccaagatcat cgtgctcgac gagccgacct
     3901 ccgcgctttc cggacccgaa tcggagcgcc tgtttggcct gatgcggcag ctcaaggcgc
     3961 gtggcacaag catgatcttc atcagccatt tcatcgaaga cgtgatggca gtttcggacc
     4021 ggatcacaat tctcaagaac tcccgaaagg tcgcggagct tgagaatacc ggtttgacca
     4081 agcgcaagct ggtcgaattg atgatcgggc gcgatgcgac tgagttggcg gagagctacg
     4141 aaggcggcac gcagctcccg ccccgggtcg acggcgaaac ggcactccgg gttgagtcgc
     4201 tttccgtgcc gggcgaattt cacgacatca cgttcgacgt caagcacggc gaaattctcg
     4261 gcatgttcgg gtacctcggt ggcggcatga cagaggtcgc gcgggcgctg tttggtcgcc
     4321 tgaagccatc acgagggacg attgttctgg acggcgaggt ggtcaggcca acgacgccga
     4381 tcaaggcgaa gcggctcggg atcgcctacc tgaccgagaa ccgccgggcg acgctcttcc
     4441 cgcgccatga gatctacaag aacatcacgc tcgcgcatct ggggcatctg gtgccgccgc
     4501 tgttttcagc acgcaacgaa atggccgtgg ccgacgagca catcaggctc actggtgtcc
     4561 gaccggcgaa ctcgcggatg cgggccgggc acctttcggg cgggaaccag cagaaggtgg
     4621 tgctggccaa gtggctcacc aggcgaccga aggtgctgat cctgaatgaa ccgacacgcg
     4681 gcatggatgt tggcgccaag cgcgaggtac tcgatttgat cagaacgctc aaggcccagg
     4741 gagtcgcgat catcctgatg tcgaccgaac cggaaaccgt gatggccgaa tccgaccgca
     4801 tcatcgtcat gtccaagggc aagatcactg ccgagttcgc ggatcaggaa gtgaccaagg
     4861 agcaattgct ggtccacgcc tgatatgcgg gtgcgtatgc tggcgcgccc tgtcggttca
     4921 catggttggg gttgggagga acacgatgag tgcggatgcg atcacagttc caggtggcat
     4981 acctggaccg cgggcgggca acccgctcct gcggtgggcg gcccgcaacg cgcgtatcgt
     5041 ggcccccctg atcacgctga tcgtggaggt catcttcttc tcgttcgcgt ctgacgtctt
     5101 cctgacccgg tccaatttcc agaatgtggt gtcgcaggta ggcccggtgg ccgttgcggc
     5161 cacgggaatc acgttcgtcc tgctctgcgc ggagatcgac ctcagcattg cgtcgatctc
     5221 ggtcttttgc gggatagtcg cgacctacat tttcagtaac gactggcacg gtctcgggca
     5281 gtggggaatc ctggttgcgg ccgcggccgg cgcggcgatc ggcctgatca acgggttttt
     5341 catctgctac gtcggcattc cgtcgttcat gatgacgctg gccatgctga cgatcgcca
//
LOCUS       DASSGX010000039         3356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_550952, whole genome shotgun sequence.
ACCESSION   DASSGX010000039 DASSGX010000000
VERSION     DASSGX010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550952"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(245..1696)
                     /locus_tag="VFI12_00595"
     CDS             complement(245..1696)
                     /locus_tag="VFI12_00595"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET7054922.1"
                     /translation="MTSISDARLARILTGPVTRRGALRGLGSAGLVAGLAEATRDRAG
                     AAEIGVDAGAPVEPEAGSWKPWVLTSGDQFRLPPPDNAETGAELDELEEWAAHREADL
                     DRIAYWDTGSPAYRWNNIAVQYLLDNKVVGLRAARSLALLNIAIADATIAAWDSKYAH
                     NRPRPVVVRPTLATAVVTPNSPAYPSEHAVTAAAAATVLGYLFPADADRFSGLAVEAG
                     RTRIEAGTDYPSDVAVGKVLGRKVAELAIDHVRDDGSDAEWTGTVPDEPGKWTGDKPY
                     EPMGGTWQTWVLTSGDQFRPGPPPAPDSVQMAKDLAELREYERTGLSNLTAAYWEYYG
                     GRASFELYSNQTSLKLFESRLDANPPRAARVYALVQAAMYDVFVAAWDAKYTYWAMRP
                     NQIDPTVVTVFTTPSHPSYPAAHASLAGAMETILGALFPQDAEYFTRMADAESWSRLW
                     AGIHFRSDIEAGRELGHNVGNAVVERASGDGAD"
     gene            complement(1891..>3356)
                     /locus_tag="VFI12_00600"
     CDS             complement(1891..>3356)
                     /locus_tag="VFI12_00600"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET7054923.1"
                     /translation="AEAVGGVESREETIDLVGSLVDQSLLRKQADAGGEPRYLTLETV
                     REYGLEQLVLAGEEAETRQRHADYYVALLESITPSPRWPATPARVRLIDAERDNLRAV
                     LAWLDAVGDAEHLLHMILRLWVLWATFGQVSEGRRWFSRILEHGDALSPFVRGMALGN
                     AGTLATFQGDGERALRLIEEGLELVETVADPTLDDRFEIALMMRQYGFTLLTLGHYEE
                     AESWVERSLTTFRELGSEENVAGSRHMLASAAYGRGNLTRAQELYEATEAQARATGSA
                     GYIASALDGRALVSCARGDYAQAANAIDEAFAQGALAGIYAGPPRRLAIVAVVAIGAG
                     EYESAARWFGAADIVAQMHGTPFALPDGPQFEGAIATVRAALGEDRFVAAWTAGRALT
                     HEEAVSQARSFLAASYLVPDSAGGDAPDPGKEHVLTHREMEVLRLVADGRSDRDIADT
                     LFIGPSTVRTHLTSIFAKLDVNSRTAAVAAARRRGIL"
BASE COUNT          592 a         1084 c         1091 g          589 t
ORIGIN      
        1 cttggcaggc ggcgatcgat ccgatcaatc gtggccagtt catgcgccgt tcgcccctcc
       61 caacatgaag cagaattcac tatgccggtg acggtcgatg cgagatccgg cacaggctac
      121 ccggctcccg gtgaaagtcg aatagcgagc accgagggaa gggtctgcag gtaccaagcg
      181 cagagcggcg agggcagcct gacggccacc cccgtcgatc gtgatcccct agcgctgccg
      241 gagttcagtc tgcgccgtcg ccggatgccc tttcgacgac ggcgttgccg acattgtgac
      301 caagttcgcg gccggcctcg atatcggatc ggaagtggat tccagcccaa aggcgcgacc
      361 aactctcggc gtccgccatg cgagtgaaat attcggcatc ctgggggaag agcgcaccga
      421 gtatcgtctc catcgctccg gccagactgg cgtgggcggc ggggtaactg gggtggctcg
      481 gcgtcgtgaa gacggttacc acggttgggt cgatctgatt tgggcgcatc gcccaatacg
      541 tgtatttcgc gtcccaggcg gcgacgaaga cgtcatacat agcagcctgc accagggcgt
      601 aaacgcgggc agcgcgcggc gggttggcgt caaggcgcga ctcgaacagc ttcaggctgg
      661 tctgattcga gtacaactcg aaggaagcgc gtccgccgta atactcccaa taggcagcgg
      721 tcaggttgga caggccggtg cgctcgtatt cgcgtagctc ggccaaatcc ttcgccatct
      781 ggacggagtc gggagccggc ggtggtccgg gccggaactg gtcgcccgac gtcaataccc
      841 acgtctgcca ggttccgccc attggctcgt agggcttgtc gccggtccac ttgcccggct
      901 catccggtac ggtcccggtc cactcggcgt cggagccatc atccctgacg tgatcaatcg
      961 ccaattcggc gaccttccgg ccgaggacct ttcccaccgc cacatcgctg ggatagtctg
     1021 tgccggcctc gatgcgagta cggccggcct cgaccgccag gccagaaaat ctgtcggcat
     1081 cggcggggaa gaggtagccg aggaccgttg ccgcggcagc agccgtgacg gcgtgctcgg
     1141 agggatacgc agggctgttg ggcgtcacca cagcggtcgc cagggtcggc cgcaccacga
     1201 ccggccgggg ccgattgtgg gcatacttgc tgtcccaggc ggcgatcgtc gcgtcggcga
     1261 tggcgatatt gagcagggcc agggaacggg cggcgcgcag gccgacgacc ttgttgtcaa
     1321 gcaggtactg gaccgcgatg ttgttccagc ggtaggccgg cgagccggtg tcccagtagg
     1381 cgatgcggtc caggtcggct tcccggtggg cagcccattc ctccaattca tccagttcag
     1441 ccccggtttc cgcgttatcc ggagggggaa gccggaactg gtcgccggag gtcagcaccc
     1501 acggcttcca cgagccggcc tccggctcga ccggcgcgcc ggcgtcaacg ccgatttccg
     1561 ctgcgccggc gcggtcgcgg gtggcctcgg ccaagccagc gacaagaccg gcactgccca
     1621 ggccgcggag tgctccgcga cgcgtgacgg ggccggtcag gattcgagca agtcgggcat
     1681 cgctgatcga ggtcatggga gtcatcctgt cctttcatgg atcggccaaa cctctcctca
     1741 cgtgctggag gtgcggaacc tgtctggcaa ccaataggtg gccagattag gtcgcggctt
     1801 gacggagcac atcagcggaa tcgaggaatt tcccggggat tggcggtgga tggaccccac
     1861 gaaaagtggg atgagtggct tcgacgctcg tcagagaatg ccacggcgac gggcggcggc
     1921 gactgcagcc gtgcgcgagt tgacgtcgag tttggcgaag atgctggtca ggtgagtacg
     1981 gaccgttgag gggccaatga agagtgtgtc ggcgatgtcc cgatcgctgc ggccgtcagc
     2041 gaccagccgc aagacctcca tctcgcgatg ggtcaggacg tgttcctttc ctggatccgg
     2101 cgcgtcgccg ccggccgagt ccggaaccag atacgatgct gccaggaaag aacgtgcttg
     2161 ggagacggct tcttcatggg ttagcgcccg ccccgcagtc cacgccgcga cgaaccggtc
     2221 ctcccccagg gcggcgcgga ctgtggcgat tgccccttcg aactggggcc cgtcgggcaa
     2281 ggcaaatggc gtgccgtgca tctgagcgac gatgtccgcg gcgccaaacc atcgagcggc
     2341 cgattcatat tccccggctc cgattgccac gacagcgacg attgccaatc gtcttggtgg
     2401 cccagcataa attccggcga gtgctccttg cgcaaacgct tcgtcgatag cgtttgctgc
     2461 ttgggcgtaa tctccgcgag cacacgatac gagcgcacgg ccatcgagcg cactggcaat
     2521 gtaccccgcg ctgcccgtcg cgcgggcctg agcctcggtc gcttcgtaca gctcctgcgc
     2581 acgcgtcagg ttccctcgtc catatgcggc cgaggcgagc atatgccggg atccggcgac
     2641 gttctcctcg ctgccaagct cccggaaggt cgtcagggac cgttctaccc aggactccgc
     2701 ctcctcgtaa tggcccagag tcagcagggt gaatccatat tgacgcatca tgagagctat
     2761 ttcgaagcgg tcgtccaggg ttggatcggc aacggtttca accagttcga gtccctcctc
     2821 gatcaaccgc agcgctcgct cgccgtcacc ctggaacgtg gcaagcgtgc cggcattacc
     2881 gagcgccatt cctcgaacga atggggagag ggcatcgccg tgttccaaga tccgtgaaaa
     2941 ccagcgacgg ccctcgctca cttggccgaa ggtggcccag agcacccaca gccgcaagat
     3001 catgtgcagc aaatgctcag catctccgac cgcgtccagc caggccagaa ctgcccgaag
     3061 attgtcgcgc tcggcatcga tcaggcgaac ccgcgctggt gtcgccggcc agcgcggtga
     3121 tggcgttatt gattccagca gcgcaacgta gtaatcggcg tgccgctgcc gggtttcggc
     3181 ctcctcgccc gccagaacca actgctccag gccgtattcc cggacagtct ccagcgtcag
     3241 gtagcgcggc tcgccgccgg catccgcttg tttgcgcaac aggctctgat caaccagtga
     3301 cccgaccagg tcgatcgtct cctcccggct ctccacccca ccgacagcct cagccg
//
LOCUS       DASSGX010000040         2688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_333470, whole genome shotgun sequence.
ACCESSION   DASSGX010000040 DASSGX010000000
VERSION     DASSGX010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333470"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="VFI12_00605"
     CDS             <1..684
                     /locus_tag="VFI12_00605"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET7054924.1"
                     /translation="WAACPRWATFQSADLVDAVEQLGGQWAVVFVDVQGSGRGGRRLA
                     STLLRMTREHLASGLTAETAVLAVHQHLHTLRQGKVGASIHVCEVDAINAQTAIAGLG
                     MLSVATSAGDEWKTNAIETTPAGFESTVEVATARCDFSAGTRIVLANDGVAHQARDLS
                     SLLTDPQEQLDPEFNAECVLLRAIQRDSGRPRSDMAVAVIEHRMADPGNRILGANVSV
                     PVKRHGSDL"
     gene            684..1064
                     /locus_tag="VFI12_00610"
     CDS             684..1064
                     /locus_tag="VFI12_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7054925.1"
                     /translation="MSAPESLGRISVVGPCASGKSTVVRALRMHGYDAHVTSQEHSAI
                     PTLWSRQGPAVLIALQVDLESVRLRRRNPRWSRDVWQAQQIRLADAFAHADLVTDTSD
                     KNANEVVRDVLEFLESRGEREESE"
     gene            1071..2687
                     /locus_tag="VFI12_00615"
     CDS             1071..2687
                     /locus_tag="VFI12_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7054926.1"
                     /translation="MTSDVRFGARSRIWRIALVALLVLGIVLGGRAERSRSADDADLR
                     LAGPALGPETLDPAQMRDLSSVTLLRQIYRGLVYFDDELNPVPELAESFDISGDGLTY
                     TFKLRPGVTFHDGSPITADDVAFSLARAVNPNTVEGDTSLLAGPTFLSDIVGFPEVYS
                     GSTDALRGIDVLSDESISITLVAPSATFLTRLASVPAAIVDRHQVETDSTWWARPIGS
                     GPYRVDRWDPGEQLLLSRYDDFVLGRPKVKTISFRLGSRALQSFNLYQAGEIDVDSVS
                     LFDVDRVTDPNGDYFDQVVRTPQFAFGYIALRTDTAPLDDSKIREALQLAFPRDKFAA
                     ITFNGKVDVANGMISNGMLGQDWAGAMPAADLDAARAAIRQSRYGSADKVPPIRIYAS
                     GALASEVMRDVASEDLGLTIEVFDLEWYDFLDRIENSQVPAYELYWAADYPDPEAILQ
                     PLWGTGRTGNYTDYSNTDVDKLLAAAATETDPGRRASQYAQAEELILADHVVIPTYMD
                     VQYTVIKPNVHGVVITPIGILRLETATIDQ"
BASE COUNT          489 a          854 c          867 g          478 t
ORIGIN      
        1 tgggcagcct gcccgcgctg ggccacgttt cagagcgccg atctggtcga cgcggtcgag
       61 cagctcggcg ggcaatgggc cgtcgtgttt gtcgacgttc agggttcagg ccgtggcgga
      121 cgccggctgg caagcacgct gctccggatg acgcgcgagc atctggcgtc aggcctgacg
      181 gccgaaaccg ccgtactcgc cgttcatcag cacctccata cgctgcgtca gggcaaggtc
      241 ggggcgtcga tccatgtctg cgaggtcgac gcgatcaacg cgcagaccgc gatcgcgggg
      301 ctgggcatgc tgagcgtagc gacatccgca ggcgacgaat ggaagaccaa cgcgatcgag
      361 acaacgcccg ccgggttcga gagcactgtc gaggttgcga cggcgaggtg cgacttcagc
      421 gccggcacac gaatcgtcct ggcgaatgac ggcgtggcgc atcaggcgcg cgacctttca
      481 tcgctgctca ccgatccgca ggagcagttg gaccctgaat tcaacgccga gtgtgtcttg
      541 ctcagggcaa tccagcggga ttccgggcgg ccgcgctcgg acatggccgt tgccgtgatc
      601 gagcaccgca tggctgatcc tggcaatcgg atcctcggcg cgaacgtttc agttccggtc
      661 aaacgacacg ggagcgatct gtgatgagcg ctccggaatc cctcggccgc atatctgtgg
      721 tcgggccgtg cgcgtccgga aaatcgaccg tggtccgggc gctgcgcatg cacggctacg
      781 acgcccatgt cacgtcgcag gagcacagcg caatcccgac gctctggagc aggcagggtc
      841 cagcggtact gatcgcactc caggtcgatc ttgaatcggt gcgcctgcgc cggcggaacc
      901 cgcgctggag ccgagacgtc tggcaagcgc agcaaatccg gctggcggat gccttcgcac
      961 acgcggacct ggtcaccgac acttcagaca agaacgccaa cgaagtcgtg cgggacgtcc
     1021 tcgaattcct cgaatcccgc ggcgaacgcg aagaaagcga gtaggcagcg gtgacgtccg
     1081 acgttcgctt cggcgcccga tcccggatct ggcgtattgc cctggtggca ctgctcgtcc
     1141 tgggcatcgt gcttggcggc cgcgccgaac gctcgcgttc cgctgacgac gcggacctcc
     1201 gcctggcggg accggccctc ggaccggaga cactcgatcc cgctcagatg cgagaccttt
     1261 cgtccgtgac cttgttgcga cagatctacc ggggcctggt ctacttcgat gacgagctga
     1321 acccggtccc cgagcttgcc gaatcgttcg atatctccgg cgacggattg acgtacacgt
     1381 tcaagcttcg gccgggcgtc acgtttcacg acggcagccc gattaccgcg gatgacgtcg
     1441 ccttttcgct ggcgcgggca gtgaatccaa acacggtcga aggcgatact tcgctgcttg
     1501 ccggcccgac atttctgtca gacatcgtcg ggttccccga ggtctatagc ggctcgaccg
     1561 acgcgttacg cggaatcgac gtgctgtcgg atgagtctat ttcgatcaca ctggtggcgc
     1621 cgagcgcgac cttcctgacg cggctggcgt ccgtacccgc tgcaatcgtc gaccgacacc
     1681 aagtggagac cgattcaacc tggtgggccc ggccgatcgg atcggggccg taccgcgtcg
     1741 atcggtggga ccccggcgag cagctcttgt tgagccgcta cgacgacttc gtgcttgggc
     1801 ggccgaaggt caagaccatc tcgttccgcc tcgggtcgcg cgcgctccag tcgttcaacc
     1861 tctatcaagc cggtgagatc gatgtcgact cagtgtcgct ctttgatgtc gatagggtca
     1921 ctgacccgaa cggtgattac tttgaccagg tcgtgcggac gccacaattc gccttcggct
     1981 acatcgcgct ccgaacggat accgcgccgc tcgacgattc gaagatccgg gaagcgctcc
     2041 agctcgcgtt tccacgcgac aagttcgcgg ccatcacctt caacgggaag gtcgatgtcg
     2101 cgaatggcat gatctcaaac ggcatgctcg gccaggactg ggcgggggcc atgcccgcgg
     2161 ctgatctcga cgcggcccgc gcggcgatcc ggcaatcgcg ctatggatca gccgacaagg
     2221 tcccgccgat tcgaatctac gcatcaggcg cgttggcatc cgaggtaatg cgagacgtcg
     2281 cgagcgagga ccttggcctg acgatcgagg tatttgacct cgagtggtac gacttcctcg
     2341 atcggatcga aaactcgcag gtgccagcat atgagctcta ttgggcagca gactacccag
     2401 acccggaggc gatcctccag cccctctggg ggacaggtag aaccgggaat tacaccgact
     2461 actcgaacac cgacgtcgac aagttgctgg ctgcggctgc gacagagacc gacccgggcc
     2521 gtcgagcgtc gcagtatgcc caggcggagg agttgatcct cgcggatcac gtcgtgattc
     2581 cgacgtacat ggacgtgcag tacacggtga tcaagccgaa cgtgcacggc gtcgtgatca
     2641 cgccgatcgg gatcctgcgg ctggagacgg cgacgatcga ccaatgac
//
LOCUS       DASSGX010000041         4999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1203997, whole genome shotgun sequence.
ACCESSION   DASSGX010000041 DASSGX010000000
VERSION     DASSGX010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4999
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1203997"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..691
                     /locus_tag="VFI12_00620"
     CDS             137..691
                     /locus_tag="VFI12_00620"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET7054927.1"
                     /translation="MADYSLISARSRPILFGALLLGLAGCGGDDMESSSLIPWPDKRA
                     TGAEPGQAAPNFVLETSDGTPLTLASLTGTQPLVVNFLATWCANCMEEMGLLVDLHES
                     GVPVLGVNLREEPETVQGLMDRTGAGFPILLDRTGKVTRAYKVVNLPATVVLAPDGRI
                     ATITRGPISADGIRESVAAALDRG"
     gene            693..1616
                     /locus_tag="VFI12_00625"
     CDS             693..1616
                     /locus_tag="VFI12_00625"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HET7054928.1"
                     /translation="MATMHSPVSVPRRRSVWLATAQALGLVGTALAAILLLAGVGGAA
                     LGEPELTGRMLTLAIPALLAGMLSILSPCSLPIVLGYFSVMFQQQPRRIGSITIVFLL
                     GVGTTMSVLGASFTALGSIAIQYQESMTRAGGVLIIAFGLLTLRGRGFGGLHLTGRTG
                     AGLGAAYLFGLMFALGWTACVGPILGSILTLLLADAATTGGAISLISGAALSQVYVLG
                     MGLPILVVVLGLMHGGNGREIGRTLRGRTLTVSLGGRIVSMPPATLLSGAMLIGLGAL
                     MLTGTMTRLSQQLGSSALSQWVVKLEAWIPT"
     gene            1660..2802
                     /locus_tag="VFI12_00630"
     CDS             1660..2802
                     /locus_tag="VFI12_00630"
                     /inference="COORDINATES: protein motif:HMM:NF006689.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HET7054929.1"
                     /translation="MSMYDLVISGGTLIDPAQGIHDRRDVAFAGGQVAAVAPCIPADQ
                     VRQTVNANGMLVVPGLVDAHVHIFEGVSHYGINADQTCLAHGATTVVDAGSAGADTFD
                     GFRKYVIEASETRILAHVNISSMGMISRQIGELDDIKWASVSKALAAIEKHRDVVLGV
                     KVRLTRESLVGAGAGLKPLFLAREAADAAGLPIMVHPQNSWAESIDQVVEVMRAGDVL
                     THMYHGDPHGILDEDGKIRPSIQAARDRGVIFDVGHGEGSFVWEIAEQALAQEFAPTT
                     ISSDLHKYNVGGPVYDLITTVAKFLHLGLTLDDALAKVTAVPARVIGLEGRAGTLAVG
                     AFGDAVVLEPQEGRFDLADSRRVSRTANLRLVPRAVIKDGRVRAGS"
     gene            3225..4931
                     /locus_tag="VFI12_00635"
     CDS             3225..4931
                     /locus_tag="VFI12_00635"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7054930.1"
                     /translation="MSGQIDKVTGSTVRSIDELMLEWEHGRLTRRSVLRRAAALGLTI
                     PALAGLTARAGSGRASAAVLNLFQEDPASGTAGGTLKIATIGEAPHLDEHQSTAEIIA
                     LIGYCAYEGLFTYDATYQAIPELVAEHTVSEDGLTHTMKLRQGVKFHNGDELKAADAI
                     ASVERWGRISGVGKRLMEKTTGITAADDYTLEWKLSEPYGTILIALAHNTQACTIHPK
                     SILDAAGDEPMTDAAQYIGTGPYKLVEWKRDSVMRFERFADYQSAGEAAQGYGGKKYA
                     YADKIEFYPVPDEAARVAGLQAGDYHIGLDIGNDQYEVLKDYDGVIAEILTPTNWDVF
                     FLNWESPMMKNLAMRQAVQLAMDPTPQLQSGRGGGDFIRLDPGLMMQQTAWYTKAGEE
                     HYNVYDPEAAKAKLQEAGYAGEPLRFMTTQEYSYMYGEAIVAKQQLEDIGITVDFRVT
                     DWATVLENRAKKDAWEMFGTGHGFVPDPSQVSYVGQMNIYPGWWSSESSLALAAELLA
                     ESDFDTRFPIWEKIQTAHYTEIPAIKIGDSSNVSFRSTALGGWDTQFERGPKFWNFWI
                     KG"
BASE COUNT          841 a         1675 c         1642 g          841 t
ORIGIN      
        1 accgtcacgc acctggccgg tccattcgtg gtcgggccgg ggcagaccta cgttcagcgc
       61 tttccgaatc ccggcatctt cccgatcaac tgcgccgtca acccggagga gtcgatcatc
      121 gtgaaagtcg agtgagatgg ccgattactc gttgatcagt gcgcggtccc ggccgatctt
      181 gttcggcgcg ctcctgctcg ggctggccgg gtgcggcggg gacgatatgg aatcgtccag
      241 cctgatcccg tggccggaca agcgagcgac cggcgccgag cccggccagg ccgcgccgaa
      301 cttcgtcctc gagacctcgg acgggacgcc gctcacgctg gcctcactga ccggcacgca
      361 gccgctggtc gtcaacttcc tggccacctg gtgcgccaac tgcatggagg agatgggcct
      421 gctcgtcgac ctccacgaga gcggggtccc ggtgcttggt gtcaacctgc gcgaggagcc
      481 ggagacggtg caggggttga tggatcgaac cggagccggc ttcccgattc tgctcgaccg
      541 caccggtaag gtgacccgcg cctacaaggt ggtcaatttg ccggccacgg tcgtgctggc
      601 gccggacggc cggatcgcca cgatcacgcg cggcccgatc tccgcggatg gcatccggga
      661 gtcggtcgcc gccgcgctgg acagaggctg agatggccac gatgcattct cctgtctctg
      721 tgccccggcg tcggtccgtc tggctcgcga ccgcgcaggc gctcggcctg gtcgggacgg
      781 cactcgccgc gatcctgctc ctggccggag tcggcggggc ggcgctcggt gagccggagc
      841 tgaccggccg gatgctgaca ctggcgattc cggccctgct tgcggggatg ctctcgatcc
      901 tctcgccctg ctcgctgccg atcgtgctcg gctacttctc ggtcatgttc cagcagcagc
      961 cacggcggat cgggtcgatc acgatcgtct tcctgctcgg cgtcggcacg acgatgagcg
     1021 tcctcggcgc gagcttcacc gcgctcggct cgatcgccat ccagtaccag gagtcgatga
     1081 cccgggccgg cggcgtgctg atcatcgctt tcgggctatt aacgttgcgc gggcgcggtt
     1141 tcggcgggct ccacctgacg ggccggaccg gggccgggct cggcgcagcc tatctcttcg
     1201 gtttgatgtt cgcgctcggc tggaccgcct gcgtcggccc gatcctcggc tcgatcctga
     1261 ccctgttgct ggcggatgcc gccacgacgg gcggggcgat ctcgctcatc tcgggtgcgg
     1321 cgctctcgca ggtctatgtc ctggggatgg gcctgccaat cctggtcgtg gtccttggcc
     1381 tgatgcacgg cggcaatgga cgagagatcg gccggacact ccggggtcgg accctgaccg
     1441 tgtcgctcgg tggccggata gtttcgatgc cgccggcgac actcctgagc ggcgcgatgc
     1501 tgatcggact cggtgcgctg atgctgaccg gaacgatgac ccggctcagt cagcaactcg
     1561 gttccagcgc cctctcgcaa tgggtcgtca agctcgaagc ctggatcccg acctgaccgg
     1621 aaccggcgcg ccgcgataga gtgccgcgga gggggagcaa tgtccatgta tgacctcgtg
     1681 atttccggcg gcacgcttat cgatccggcg cagggtattc atgaccggcg cgacgtcgcc
     1741 ttcgccggcg gccaagtcgc ggcggtcgcg ccctgcatcc ctgccgatca ggtgcggcag
     1801 acggtcaacg ccaacggcat gctggtcgtg ccgggcctgg tggacgcgca cgtccatatc
     1861 ttcgagggcg tctcgcacta tggaatcaac gccgaccaga cctgccttgc ccacggcgcg
     1921 acgaccgtcg tcgatgccgg atcggccggg gcggacacct tcgacggctt tcggaagtac
     1981 gtgatcgagg cgagcgagac ccgcatcctc gcccacgtca acatctcgtc gatgggcatg
     2041 atctcgcgcc agatcggcga gctggacgac atcaagtggg cgagcgtgtc gaaggcgctg
     2101 gccgcgattg agaagcaccg cgacgtcgtc ctcggtgtca aggtgcgcct gacccgggag
     2161 tcgctcgtcg gcgccggggc cgggctgaag ccactcttcc tggcccggga ggcggccgat
     2221 gcggccggtc tgccgatcat ggtccacccg cagaactcgt gggcggagtc gatcgatcag
     2281 gtggtcgagg tcatgcgcgc cggggacgtc ctgacgcaca tgtaccacgg tgacccccac
     2341 ggcatcctcg atgaggacgg caagatccgg ccgtcgatcc aggctgcgcg cgatcgcggc
     2401 gtgatcttcg atgtcggcca cggcgagggc tccttcgtct gggagattgc cgagcaggcg
     2461 ctcgcccagg agttcgcgcc gaccacgatc tcgtccgacc tccacaagta caacgtcggc
     2521 ggcccggtct acgacctgat cacgaccgtc gcgaagttcc tgcacctcgg cctgacgctc
     2581 gacgacgccc tcgcgaaggt gaccgccgtg ccggccaggg tgatcggcct cgagggcagg
     2641 gccggcacac tcgccgtcgg cgccttcggc gatgcggtcg tcctcgaacc ccaggagggc
     2701 cgcttcgacc tggcggactc gcgccgcgtc tcccgcaccg ccaacctccg cctggtcccc
     2761 agagcggtca tcaaggacgg ccgagtacgg gcgggaagtt gagggtggag ggaatgttgt
     2821 aggggcagat ggcatctgcc cgtgcctctc cccaagaccc gttcgcatgc accggcgcgg
     2881 gtcctgatcc gtggacccgg acccgcgctt tccggacggc ggcgggcaga tgccatctgc
     2941 cactacaaac ctccacgcct ccacgccccg tcttctcgac ttcgcacctc cacgcctcct
     3001 cgcctcctcg cctcctcgcc tccgtcaact gtcgacaatc cctgtttcca aatttctcaa
     3061 catcgggtag catgccgaca aggcaacgga cgaagcgaat gtgcgagcag aacggcggca
     3121 tacgatcgag acgcgatata cgtgtcgacg atcatgaacc gccaaaatct ggtcaccatt
     3181 gcttcccgcg ttgcgccgtt cgcagtcagg ttggagggac aacgatgtcg ggtcagatag
     3241 acaaggtgac gggttcgacc gtgcggtcga tcgacgagct gatgctcgaa tgggagcatg
     3301 gccgcctcac tcgtcgttca gtactccggc gcgccgccgc gctcggattg acgattccgg
     3361 ccctcgccgg gctcacggcg cgcgcgggca gcgggcgagc gtccgccgcg gtgctcaacc
     3421 tcttccagga ggatccggcg tccggcacgg cgggcggcac gctcaagatc gccacgatcg
     3481 gcgaagcgcc gcatctcgac gagcaccagt cgacagccga gatcatcgcg ctgatcggct
     3541 actgcgcgta cgagggcctc ttcacgtacg acgcgaccta ccaggcgatc cccgaactgg
     3601 tcgccgagca caccgtcagc gaagacggcc tgacccatac catgaagctc cgccagggcg
     3661 tcaaattcca caatggcgat gagttgaaag cggcggacgc gatcgcctcg gtcgagcgct
     3721 ggggtcggat cagcggcgtc ggcaagcgcc tcatggagaa gacgaccgga atcaccgcag
     3781 ccgacgatta cacgctcgag tggaagttgt ctgagcccta cggcacgatc ctgatcgcgc
     3841 ttgcccacaa cacccaggcc tgcacgatcc acccgaagtc gatcctcgat gccgccggcg
     3901 atgagccgat gaccgacgcc gcccagtaca tcggcaccgg cccctacaaa ttggtcgagt
     3961 ggaagcggga ctcggtcatg cgcttcgagc ggttcgccga ttatcagtcg gcgggcgaag
     4021 cggcgcaggg gtacggcggc aaaaagtacg cctacgccga caagatcgag ttctacccgg
     4081 tgccggatga agcggctcgc gtggcgggtc tgcaggccgg tgactatcac atcggactgg
     4141 acatcggcaa tgaccagtac gaagtcctca aggactacga cggcgtcatc gccgagatcc
     4201 tgactccgac caactgggac gtcttcttcc tgaactggga atcgccgatg atgaaaaacc
     4261 tggccatgcg ccaggcggtc cagctggcaa tggacccgac cccgcagctg cagtctggcc
     4321 gcggcggcgg cgacttcatc cggctcgacc cgggcctgat gatgcagcaa acggcctggt
     4381 acaccaaggc cggcgaagag cactacaacg tctacgaccc ggaggcagcc aaggccaagc
     4441 tgcaggaggc cgggtacgcc ggtgaaccac tccggttcat gacgacccag gagtactcct
     4501 acatgtacgg cgaggcgatc gtcgccaagc agcagttgga ggacatcggc atcacggtcg
     4561 acttccgggt caccgactgg gcgacggtcc tcgagaaccg ggccaagaag gatgcctggg
     4621 agatgttcgg caccgggcac ggcttcgtgc cggatccgag ccaggtctcc tacgtgggcc
     4681 agatgaacat ctatccgggc tggtggagct cggagagcag cctcgccctg gccgcggaac
     4741 tgctcgccga atccgacttc gacacccggt tccccatctg ggagaagatc cagacggctc
     4801 actacacgga gatcccggcc atcaagatcg gcgactccag caacgtctcg ttccgatcca
     4861 cagcgctggg cggttgggac acgcagtttg agcgtggtcc gaagttctgg aacttctgga
     4921 tcaagggata gcttcccggt ccttgcggcc attcggggcg tgccatacca cggcacgccc
     4981 cgaatagtta ccagtctcg
//
LOCUS       DASSGX010000042         2551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1531428, whole genome shotgun sequence.
ACCESSION   DASSGX010000042 DASSGX010000000
VERSION     DASSGX010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1531428"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            634..2412
                     /locus_tag="VFI12_00640"
     CDS             634..2412
                     /locus_tag="VFI12_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7054931.1"
                     /translation="MAKPRNPRPQDEPKVTGVDFASEVRTGEDTRQRGWFWHWNELHT
                     EYEPLLKHSGIGLITSYIVWTDRREHSPYRGYAFPSLQSQAAFSGSDRAELITINRIL
                     VALDLLEIRKEMVLRVDAQGHKWRVPHNLYRVKDRSGDPHLTARDVTRVLELAEERKD
                     VYRHIRHILTSSFVPISRTNVWHQILAELRPTPLWQRLSAQALAEEARFSARSKAGHK
                     SRKSADEAGADPERPVNARTVQPVEDVELDADFFIPGSEDTQATATVVSDQGSASVEM
                     TVATSVAPTNDGCQTSVAASNQGFAKDRKSSDGESNEGQPTSVAPSNSMYDQSIQTTR
                     TRKGNSGQGIEEAVTRDSEAVTGIVTTAGVALSQVGSGPGNGDAPANGPDRAAALTAF
                     VEANGRTPTAAEERLLGGIADEAGSEHGWAIVTAAIYEAVDSGSAYVAPKRIREIVRR
                     WLRDGLPEDIAGAPGPEIGIPAAVEHDSPATDDDLDGLTENRPFWVAEAGIGSVQLWA
                     AVVDLVAQRGDARPAEIHDYLKPAVLLERIGPAALRLGVPHDLARQRIERRWRTALED
                     AFAQLLGGAGWELEIEVMGTPRRRSA"
BASE COUNT          465 a          841 c          845 g          400 t
ORIGIN      
        1 cgcgtggctg ctccctaaga tgggccgagg cgatccccgc tccaaacgac agtccgtgaa
       61 aatagacggc gtctcatttt cacgatcgct gggtcaggac cggggcgcga ttcaccgtgg
      121 cggaatcaat cgccagaatt gtgtagattt agggggacac ggccgccgca gtccagcatc
      181 gagactggag ccgggggacc agggaggaat tgatgccaga cgcgaccgga acgatgggga
      241 gccggatcgg cgccaaccga tcagcgcttc gcatcccggc aaacgaaaag gcgatcaacg
      301 cgccaacgtc gatcgcctgc tctgctccgg tccaaagaac cgtcgtttgt tcgtcgtttc
      361 ccggttgagc gcgaacggtc cgtcgccaaa cagctgctcg cctcgccggt tctgatcaat
      421 cgccatcgtc cccgccaagg gagggtttcg tgtacccggg cgacctgttg tgcattctag
      481 tcacgccggt ttcggcgtgt caagcggttg cggtgtgtcg cggcggaatc cgccgggcat
      541 tcccggctgc cgcatgccac cacgcccgca cgaccgcgcc ccggggccag tcggcgatcg
      601 ccaggaccgc gcggcgatgg ggagggccgg ccgatggcta agccgcgcaa tccccggcca
      661 caggacgagc cgaaggtcac cggcgtcgac ttcgccagcg aggtccgcac cggcgaggac
      721 actcgccagc gcggttggtt ctggcactgg aacgagctcc acaccgagta cgagccgctg
      781 ctcaagcact ccgggatcgg cctgatcacc tcctacatcg tctggaccga ccgccgcgag
      841 cactcgccct accgcggcta cgccttcccg agcctccaat cgcaggccgc cttctcgggg
      901 tcggaccggg ctgagctgat cacgatcaac cggatcctgg tcgcgctcga cctgctcgag
      961 atccgcaagg agatggtcct ccgggtcgat gcgcagggcc acaaatggcg cgtgccacac
     1021 aacctctacc gggtcaagga ccgctccggc gatccgcatc tgaccgcgcg ggacgtgacc
     1081 cgcgtgctcg agctggctga ggagcgcaag gatgtctacc gccacatccg ccacatcctg
     1141 accagcagct tcgtgccaat cagccgcacc aacgtctggc accagatcct cgccgagctc
     1201 cggccgaccc cgctctggca gcgcctctcg gcccaggcct tggcggaaga agcccgcttc
     1261 tcggcccgga gcaaggccgg ccacaagtcc cgcaagtccg cggacgaggc cggcgccgat
     1321 ccggagcgac ccgtcaacgc acgcacggtc cagccggtcg aggatgtcga gctggacgcc
     1381 gatttcttca tcccgggtag cgaggatacg caagcgacag cgactgtcgt tagcgatcag
     1441 ggttcagcat cggtcgaaat gaccgtggcg acctcagttg caccaaccaa cgatggttgc
     1501 caaaccagtg ttgcagcgag caatcaaggt ttcgccaaag atcgcaaaag ctcggatgga
     1561 gagtccaacg aaggtcagcc gacctcagtt gctccttcca actcgatgta tgaccaatca
     1621 atacaaacaa cgaggacgag aaaagggaat agcggacagg ggatagaaga ggctgtgaca
     1681 cgcgattccg aggctgtcac aggtattgtg acaacggcgg gggtggcgtt gtcacaggtc
     1741 ggatccgggc cgggaaatgg cgatgcgccg gcgaatgggc cggatcgcgc ggccgccctg
     1801 accgccttcg tcgaggccaa cggacgcacg cctacggccg ccgaagagcg gttgctcggc
     1861 gggatcgccg atgaggcggg aagcgagcac ggctgggcga tcgtgaccgc ggcgatctac
     1921 gaggcggtcg attccggcag cgcctatgtc gccccgaaac gaatccggga aattgtccgg
     1981 cgctggctgc gggacgggtt gccggaggac atcgccgggg cgccgggacc ggagatcggg
     2041 atacctgccg cggtcgagca cgattcacca gcgaccgatg atgacctgga tggcctgacg
     2101 gagaaccggc cattctgggt ggccgaggcc gggatcggtt ccgtccagct ctgggccgcc
     2161 gtcgtcgacc tggtcgcgca gcggggcgat gctcgtccgg ccgagatcca tgattacctc
     2221 aagccggctg tcctgctgga gcggatcggt ccggctgcgc tgcgcctcgg tgtcccccac
     2281 gaccttgccc gccagcgcat cgagcgccgc tggcgcaccg ccctcgagga cgccttcgcc
     2341 cagctcctcg gcggcgcggg ttgggagctc gagatcgagg tgatgggtac cccgaggcgc
     2401 cgatcggcgt agaacagggc gcattcttcc tatgcaccgg caccctgaac gtagcgcggt
     2461 ccccccgtgg gccgcatcat gcctctccgg acccgataga tggtgccgga tcggtgacga
     2521 cggttggttc ccgtggacgg tcgtggttgg t
//
LOCUS       DASSGX010000043         2823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1204590, whole genome shotgun sequence.
ACCESSION   DASSGX010000043 DASSGX010000000
VERSION     DASSGX010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1204590"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VFI12_00645"
     CDS             <1..483
                     /locus_tag="VFI12_00645"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HET7054932.1"
                     /translation="SFSSSYFQTPGRFNFLDVDGRRVVMDYCHNVAGLESMADFVKRL
                     GAPHAVGMIAMPGDRLDTDIEAFGDLAARTFDRLVIREDTNRRGRKDGEIAEILRQTA
                     LSAGLTDDRVHVILDEIDAVNAAVELAERDDLVVLLVDKPAAAWRELEKRVSLRGELV
                     "
     gene            complement(480..2042)
                     /locus_tag="VFI12_00650"
     CDS             complement(480..2042)
                     /locus_tag="VFI12_00650"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HET7054933.1"
                     /translation="MKLVEIRDLDGPNLFMLEPAIKVEIQLEDGEAQSEIARRLDSDY
                     SDLRAAAESFLARAGHASVVVSRRMDSPGHISIAFSWRSRAKALDVAGQMAAAMLSVV
                     DDNIPGCITELTVSGGVADMPECLPDSQRSAIAIAVTGTNGKTTTTRLTAHLMQSAGY
                     RTGWNSSSGIYIDGEEIEAGDYSGPSGARRILDDPSVQAAVLETARGGILLRGLGYAH
                     NDVSVFTNVSADHLGLQGVLTLETMAEVKAVVCKVTRPGGVAVVNADDPLVMAATNAI
                     AAPRTLVTQRPDNLLVMEHMTAGGTTIIADEREIVVRRGETVAARFILDEIPMTYAGR
                     ARHMVENAMCAIGAALGGGLGSAQIRSGLRTFRNDAAGNLGRMNIYRSNDVTFVLDFA
                     HNEAGITHLVDFGRRLLDGDGRLISIIGTAGDRTEASLKAIGRIAAEASDIVIAHGTV
                     KYLRGRTLDELLGLYRSGVELHPETEYHESETELTGLEDAITLARPGDVIVMMAHEFL
                     PQLTSRLKEIAD"
     gene            complement(2093..>2823)
                     /locus_tag="VFI12_00655"
     CDS             complement(2093..>2823)
                     /locus_tag="VFI12_00655"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HET7054934.1"
                     /translation="LLGGLGLLLPLFPPVRKLLAQVGDFDPNDPVHMLGTGIFLGALG
                     FLGGQLISAPAPDEVGSFDVGPIVAQGIFFVALALIAVGYHIDRTWPQAVKRLGLVRP
                     TWVGFGMAVAAMFVGFVTSGVASVLTRATNEQYANEINRSLEVMTTSSPSYIAAPLIG
                     LSAGIGEELLFRGALQPRYGIILTSLLFALLHSQYGFSFITLGTFLLGCIFGILAKRY
                     GTTHAIIAHALYNTIAVILSALAD"
BASE COUNT          522 a          876 c          916 g          509 t
ORIGIN      
        1 agcttctcgt ccagctattt ccagacgccc ggccgcttca atttcctgga tgtcgatggc
       61 cggcgcgtcg tgatggacta ctgccacaac gtggccgggc tcgaatcgat ggccgacttc
      121 gtgaaacgac tcggcgcgcc gcatgcagtc ggcatgatcg cgatgccggg agatcggctc
      181 gataccgata ttgaagcgtt tggcgacctt gcggcgcgga cgttcgaccg gctcgtgatt
      241 cgtgaggaca cgaaccgccg cgggcgaaag gacggcgaga tcgccgagat actccgccag
      301 accgcactgt cggccggact cacggacgat cgcgtccacg tgatcctcga cgagatcgat
      361 gccgtcaacg ccgcggtgga gctggccgag cgcgacgacc tggtagtgct gctggtcgac
      421 aagccggccg cagcctggcg cgagctcgaa aagcgggttt ccttgcgcgg cgaactcgtc
      481 tagtctgcga tttccttgag ccgtgacgtc aattgcggca ggaactcgtg cgccatcatg
      541 acgatcacgt cgccgggtcg ggcgagcgtg atcgcgtcct cgagtcctgt caattcggtt
      601 tcgctctcgt ggtactcggt ttccgggtga agttccacgc ctgaacggta gaggcccaga
      661 agctcatcca gggtccggcc ccgcaggtat ttgaccgtgc cgtgggcgat gacgatgtcg
      721 gaagcctcgg cggcgatccg gccgattgcc ttgagcgatg cctcggtacg atcgccggcc
      781 gtgccgatga ttgagatcag tcgcccgtca ccatccagta atcgccggcc gaagtcgacg
      841 aggtgcgtga taccggcttc gttgtgggcg aaatcgagga cgaacgtgac atcgttgctt
      901 cggtagatgt tcatgcggcc gaggttgcca gccgcgtcgt tccggaacgt gcgcagtcca
      961 gatcggattt gggcggaacc gagcccgccc ccaagtgcgg cgccgatcgc gcacatcgcg
     1021 ttctcgacca tgtgccgggc tcgacccgcg taggtcatcg ggatctcgtc gaggatgaac
     1081 ctggcggcca cggtctcgcc gcgccgcacg acgatttctc gctcgtccgc gatgatcgtc
     1141 gtgccaccgg ctgtcatgtg ctccatgacg agcagattgt ccgggcgctg tgtgacgagc
     1201 gttcgcggcg cggcgatggc attggtagcc gccatgacga ggggatcgtc ggcattgacg
     1261 accgcgacgc ctccgggccg ggtgaccttg cagaccaccg ccttgacttc ggccatggtc
     1321 tcgagcgtca ggacgccctg gaggccgagg tggtcggcgc tgacattcgt gaagaccgag
     1381 acgtcgttgt gcgcataccc cagtccccgg agcaggatgc cgccgcgcgc agtctcgagg
     1441 accgccgcct gcaccgaagg gtcatcgagg atgcgacgcg ccccggacgg gccggagtag
     1501 tcacccgctt cgatctcttc gccgtcgatg tagattccgc tcgacgagtt ccacccggtg
     1561 cgatagcccg cggattgcat gaggtgcgcg gtgagccgtg tcgtggtcgt cttgccgttc
     1621 gtaccggtaa cggctatcgc gattgccgac cgttggctat caggcaggca ctcgggcatg
     1681 tcggcaaccc cgcccgacac cgtcaattcg gtgatgcaac cagggatgtt gtcgtcaaca
     1741 acgctgagca tcgcagccgc catttgcccc gcgacgtcca acgccttagc acgacttcgc
     1801 caggaaaacg cgatcgagat gtggcccggc gaatccattc gccgagaaac gacaaccgat
     1861 gcgtgcccgg cgcgtgccag gaaggattct gccgccgcgc ggagatcgga atagtctgaa
     1921 tcgagacgcc gcgcaatctc gctctgtgcc tcgccgtcct caagctggat ctcgactttg
     1981 atggcgggtt cgagcatgaa caggttcggc ccatccaggt cccggatttc gactagcttc
     2041 atccctgtcc cgcgatcagc tcagtaccgg tcagcgcgtg gcggcgattg tactagtcgg
     2101 ccaacgcgga gaggataacg gcgatcgtgt tgtagagcgc gtgggcgata atcgcgtggg
     2161 tcgtaccgta ccgcttggcg aggatgccga agatgcagcc gagcaggaat gtcccgagcg
     2221 tgatgaatga gaagccgtat tggctgtgca gcagcgcgaa cagtagcgac gtcaggatga
     2281 tgccatagcg cggctgcagc gccccgcgaa acagcagctc ttcccctata ccagccgaca
     2341 aaccgattag tggcgccgcg atgtatgagg ggctcgacgt cgtcatgact tcgagcgatc
     2401 ggttgatctc gttcgcgtac tgctcgttcg tggctcgcgt cagtaccgac gcaactccgg
     2461 acgtgacaaa ccccacgaac atcgccgcga ctgccatgcc aaatccaacc cacgtcggac
     2521 ggaccaggcc gaggcgcttg actgcctggg gccaggttcg atcgatgtga tatccgacgg
     2581 caatgagagc aagcgccaca aaaaagatgc cctgcgcgac gatcggcccg acatcgaagc
     2641 tgccgacctc atcgggcgcc ggcgccgaga tcagttgccc gccaaggaaa cccagcgcgc
     2701 caagaaagat gccggtcccg agcatgtgga ccgggtcatt cgggtcaaaa tcgccgactt
     2761 gcgccagcag tttgcgaacc gggggaaaca ggggcaagag aaggccgagt ccgccgagga
     2821 gca
//
LOCUS       DASSGX010000044         2026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_770255, whole genome shotgun sequence.
ACCESSION   DASSGX010000044 DASSGX010000000
VERSION     DASSGX010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770255"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="VFI12_00660"
     CDS             <1..755
                     /locus_tag="VFI12_00660"
                     /inference="COORDINATES: protein motif:HMM:NF012341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HET7054935.1"
                     /translation="VAMEQISAVYQAAVEICREKYGMRMLVADEGGLAPDFASVDEAL
                     GDAVAAIERAGLRRGIDIALCLDVAASHFAFDSRYRLDGHLLSSAEMIEVIAGWADSY
                     PILSIEDGLAEDDWDAWPKLRRRLRNQVMVLGDDFLTTNPDRIRRAVKTRAANALLLK
                     VNQIGTLTEAAEALRLARDANWAVVVSARSGETEDDWLTDLAIGWGGDQLKVGSVTRS
                     ERLAKWNRLLTIADDTGWTLCPWPNSTPVRML"
     gene            766..1023
                     /locus_tag="VFI12_00665"
     CDS             766..1023
                     /locus_tag="VFI12_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7054936.1"
                     /translation="MEESSVIELSESGAAVARARDELLGILMKSAHTSHQPQELIRQV
                     AGNRDRDAYRAALMSLLASGVVERADGWTIRLSSGRISSDS"
     gene            1028..>2026
                     /gene="dgt"
                     /locus_tag="VFI12_00670"
     CDS             1028..>2026
                     /gene="dgt"
                     /locus_tag="VFI12_00670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HET7054937.1"
                     /translation="MRYRRRHDSTVKPASADSRSEARRDRDRIIYSIEFQRLVGVTQV
                     VSPTERFPVHNRLTHSLKVAQVGRSIAEQLLYRYRDIGIARHLDPDVVDAACLAHDLG
                     HPPFGHNTERELDTLLRTPGAADTAIEDGFEGNAQSFRVITKTAIRYQEVEQKPGLNL
                     TAASLSALLKYPWGQRENGARRHKWGYYASEREDFAFARTECGFPLESERRSLEATIM
                     NLADDISYAVHDVEDFVRAGLIPTAVFTPESNEQAWFQTKCAETTPHLSSAAVEQVLG
                     FVNLFRYKGRHTDLAMLSEFRSESVNHFITSLRIKLTNQGPEMEVPPDVRAEIDVLQ"
BASE COUNT          383 a          684 c          612 g          347 t
ORIGIN      
        1 acgtcgcgat ggagcaaatc agcgccgtct accaggccgc cgtcgagatc tgccgcgaga
       61 agtacgggat gcgcatgctc gtcgccgacg agggtggcct cgcgccggat ttcgcctcgg
      121 tcgacgaggc gctcggtgac gcggtggcgg caatcgaacg agccggcctg cgccggggga
      181 tcgacatcgc cctctgtctc gacgtcgccg ccagccactt cgcgttcgac agccggtacc
      241 gactcgacgg ccatctgctc tcgagcgccg agatgatcga ggtcatcgcc ggctgggccg
      301 attcctatcc gatcctgagc atcgaggacg gcttggccga ggacgactgg gatgcctggc
      361 cgaagctccg gaggcggctc cgcaaccagg tcatggtcct cggcgacgac ttcctgacca
      421 ccaacccgga ccgcatccgc cgcgcagtca agaccagggc cgcgaacgcg ctgctgctca
      481 aggtcaacca gatcggcacc ctgaccgagg ccgccgaagc gctccgcctg gcccgggatg
      541 cgaactgggc cgtcgtcgtc agcgcccgct ccggcgagac cgaggacgac tggctgaccg
      601 acctcgccat cggctggggc ggcgatcagc tcaaggtcgg ctccgtgacc agatccgagc
      661 gcctcgccaa gtggaaccgc ctcctgacca tcgccgacga taccggctgg accctctgcc
      721 catggccgaa tagcacaccc gttcgaatgc tatgattgct ggtacatgga ggagagctcg
      781 gtgattgaat tgagtgaatc cggagcagcg gtcgcgcgag cccgtgacga gctccttggc
      841 attttgatga aatctgccca cacctcgcat cagccccaag agctcatccg ccaggttgcc
      901 ggcaacaggg atcgcgatgc ctatcgcgcc gccctgatgt cgctccttgc aagtggcgtc
      961 gtcgagcgtg cagacggatg gacaatccgg ctcagcagcg gtcgcatttc ctccgactcc
     1021 tagctccatg cggtatcgcc gccggcacga ctcaacagtc aagccagctt cagccgactc
     1081 ccgttccgaa gcgcgccggg accgggaccg cattatctac tccatagaat tccagcggtt
     1141 agtcggcgtg acccaggtcg tgtcaccaac tgaacgcttt ccggttcaca atcggctgac
     1201 ccattcgctc aaggtggcac aggttggccg ctcaatcgcc gagcaactcc tgtatcgcta
     1261 ccgggacatc ggcatcgcgc gccatctcga tcctgacgtc gtcgacgccg cctgtctggc
     1321 ccatgatctc ggccatcccc cttttgggca caacaccgag cgcgagctcg acacgctcct
     1381 ccgtacacca ggcgcggcgg ataccgcgat cgaggacggg ttcgagggaa acgcgcagag
     1441 ttttcgggtc attacgaaga ccgccattcg gtatcaggaa gtcgagcaga agccgggcct
     1501 gaatctgaca gccgccagcc ttagtgccct cctcaagtac ccatggggcc agcgagaaaa
     1561 cggtgctcga cggcacaagt ggggctatta cgcgtcggag cgggaagatt tcgccttcgc
     1621 ccggactgag tgcggatttc cgctggaaag cgagcgccgg tcactcgaag ccacgatcat
     1681 gaacctcgcg gacgacattt cgtacgccgt gcatgacgtc gaagacttcg tgcgcgccgg
     1741 gctgatcccg acggctgtct tcacccctga gtcgaacgag caagcctggt tccagacaaa
     1801 gtgcgccgaa acgacaccgc acctctcatc cgccgccgtc gagcaagtac tcggcttcgt
     1861 caacctcttc cggtacaagg gtcggcatac ggacttggcc atgctctccg agttccggtc
     1921 ggagtcggtc aaccacttca tcacgtccct gcgaatcaag ctgaccaatc agggcccaga
     1981 gatggaggtg ccgccggacg tccgggccga gatcgacgtg cttcag
//
LOCUS       DASSGX010000045         5017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_661531, whole genome shotgun sequence.
ACCESSION   DASSGX010000045 DASSGX010000000
VERSION     DASSGX010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661531"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VFI12_00675"
     CDS             complement(<1..306)
                     /locus_tag="VFI12_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET7054938.1"
                     /translation="MFSDAFANGLWIVPVLAILILVHELGHFFAARACGVKVEEFGIG
                     IPPRLFGWTRNGVIWSINAIPFGGFVRVKGEDGANMDSDSMNAKPPLQRAFFLAAGAG
                     "
     gene            complement(406..2277)
                     /locus_tag="VFI12_00680"
     CDS             complement(406..2277)
                     /locus_tag="VFI12_00680"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7054939.1"
                     /translation="MRTIRISPWSTLLALLLIAILVGACGGSSTSKEPTSTPAPMPTR
                     AITPTSVASPIASPAASPLASPVGSPIASPMASPVGSPVALTAGGSTMTRTEFKSALL
                     SAFPMDAPQKQGGTVVLGSNGDISTTNPLLASDSTTINLLGQVFEPLLGASPIDGRPV
                     PALADSWTVSDDGLIYTFKLNQDAKWQDGVDVTADDVAFSFDAVLDPNLNSQYRSQVR
                     EVVKSYRVVDADTFEITATDRFVTFLYNAPASVFIVPKHIWGSVPFEQWSFDGGSTGE
                     DLSRIVGTGPFLFKAWEPGQRVTLAKNPNYYDTVPSIDEFAMVVQPETDTLVRSLESG
                     DVDVVEILPAPDTATIQNTSGLKVEIYDIYQITYFVMNMDGKRVPAFSDQRVRQALYQ
                     ALDRQSITNNIFLGFGEPAVGTQPPLSPSYDPSRMQPSYAYDPSAAKQLLADAGWTDT
                     NGNKTVDKDGHDLKLTITYVGGDATVDSMLAYMQEAWKQIGVDVELQNISGDLLQQQL
                     YDGSFDMSLLAISLTPDGSQGLLFTCNATTTGFNFGAYCNQDYDALDAQQLREFDPAR
                     RAQMQVQLSEMIWRDLPIGPIRFGVARTGYSTTLHNFFPNGYGFLWSLPFVWVDSGS"
     gene            2545..3657
                     /gene="ald"
                     /locus_tag="VFI12_00685"
     CDS             2545..3657
                     /gene="ald"
                     /locus_tag="VFI12_00685"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HET7054940.1"
                     /translation="MIVGIPREIKDRENRVSTTPAGVGEYVARGHTVLVECDAGVGSG
                     FANNEYERAGATMVTAHDAVFAQADMIVKVKEPIPSEYDLLRENQLLFTYLHLAAEEE
                     LTHALMRRKVKAVAYETVQLGTGSLPLLTPMSEIAGRMSIQVGAHYLERTYGGEGLLL
                     GGVAGVPGAEVVVVGGGVVGTNAAQMALGMGANVTIIDKNVDRLRYLDQVLHGRCHTL
                     ASNARNLHDKIALADFVVGGVLLPGAKAPKLVTREMIESMKPGSVVVDVAIDQGGCFE
                     TSKPTTHSDPVYDVKGVIHYCVTNMPGAVARTSTLALSNVTLPYGLALADMGLVDAAR
                     AFPELAKGINVLNGVVTYKAVADAFGLPSEPWDVVA"
     gene            3719..4639
                     /locus_tag="VFI12_00690"
     CDS             3719..4639
                     /locus_tag="VFI12_00690"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HET7054941.1"
                     /translation="MDEDLKSAVQQFLTALEADRGFAVNTIAAYRNDLEQFCAYVQNP
                     PQEDHIPAIAQWSELTEGHLSTYILHLRGREYAASTIARKTAALKSFSAHLVDRGVTR
                     VDAAEKLSAPRVEKYTPRSISPAEIDQLLAQTARAAAENRPEGLRDQAMLETLYSTGM
                     RVSELVALNLPDIDLERRVAKCTGKGGRQRSVPLRTSAINALRNYLEHGRPRIAPNEE
                     QALFVNHRGSRLTRQGFWLILKAYARQAGIQDITPHTLRHSFATHALRHGYELRDVQQ
                     MLGHVSISTTQVYRRIAVPTPAVEPAASGE"
     gene            4664..>5017
                     /locus_tag="VFI12_00695"
     CDS             4664..>5017
                     /locus_tag="VFI12_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HET7054942.1"
                     /translation="MSQSVRAASMDKIVALAKRRGFVFPGSDIYGGLANTWDYGPLGT
                     ELKNNVKQLWWRTFVQLRRDMVGLDAGILMHPRVWEASGHVVNFNDPLVDCKTCKSRF
                     RADHLIEEKLGIHAEG"
BASE COUNT          928 a         1549 c         1667 g          873 t
ORIGIN      
        1 gccggcgccg gcggccagga agaaggcgcg ctggagcgga ggcttggcgt tcatggagtc
       61 ggaatccatg ttcgcgccgt cttcgccctt gacccgcacg aatccgccga acgggatggc
      121 gttgatcgac cagatcacgc cgttccgcgt ccagccgaag aggcggggcg ggatgccgat
      181 gccgaattcc tcgaccttga cgccgcaggc gcgtgcggcg aagaagtggc cgagttcgtg
      241 gacgagaatc agaatcgcga ggacggggac aatccagagc ccattcgcga aggcgtcaga
      301 gaacaatcag ggagctcctt tgcgaccaga ccgaaacggc gcgagcggtc gcgtgctggt
      361 tcaatcctac catccagccg cgcgttgaac ggccgaaaag agttgtcatg atcccgaatc
      421 gacccagacg aatggcaggc tccagaggaa cccgtagccg ttcgggaaga agttgtggag
      481 ggtcgtgctg tagccggtcc gggccacgcc gaaccggatc ggtccaatcg gcaggtcccg
      541 ccagatcatc tccgagagct ggacctgcat ctgcgcgcgc ctggccgggt cgaactcccg
      601 caattgctgg gcgtcgagcg catcatagtc ctgattgcaa tacgccccga agttgaatcc
      661 ggttgtcgtt gcattgcagg taaagaggag gccctggctc ccgtccggcg tcaggctgat
      721 agccagcagg gacatgtcga agctcccgtc atagagctgt tgttggagca ggtcgccgct
      781 gatgttctgc agctcgacgt cgacgccgat ctgcttccag gcttcctgca tgtaggccag
      841 catcgaatcg accgtcgcat caccaccgac ataggtgatc gtcagcttga ggtcgtgccc
      901 gtccttgtcg accgtcttgt tgccgtttgt gtcggtccag ccggcatcgg ccagcagctg
      961 cttcgcggca ctcgggtcat acgcgtacga gggttgcatc cgggatgggt cgtaggacgg
     1021 cgagagcggc ggctgcgtgc cgacggccgg ctcgccaaag ccgaggaaga tgttgttggt
     1081 gatcgattgg cggtcgagcg cctggtacag cgcctggcgg acgcgctgat cggagaaggc
     1141 cggcactcgc ttgccgtcca tgttcatcac gaagtaggtg atctggtaaa tgtcgtagat
     1201 ttcgaccttg agtcctgacg tgttctgaat cgtggccgta tccggcgccg gcaggatctc
     1261 gaccacgtcg acgtccccgg actccagcga gcgcaccagg gtgtctgtct ccggctgcac
     1321 gaccattgcg aactcatcga tgcttggcac cgtatcgtag tagttggggt tcttcgcgag
     1381 cgtcacccgc tggccaggct cccacgcctt gaacaggaac gggccggttc cgacgatccg
     1441 ggacaggtcc tcgccggtgc tgccgccgtc gaagctccac tgctcgaacg gtaccgaccc
     1501 ccagatgtgc ttcggcacga tgaaaaccga ggcgggcgcg ttatagagga acgtcacgaa
     1561 ccggtctgtc gccgtgatct cgaacgtgtc ggcatccacc acgcggtagg acttgacgac
     1621 ctcgcgcacc tgcgaccggt actggctatt caggttcggg tcgagtaccg catcgaagct
     1681 gaaggcgacg tcgtcggccg tgacgtcgac accatcctgc cactttgcgt cctggttcag
     1741 cttgaacgtg tagatcaatc cgtcgtcact gacggtccag ctatcggcga gggccgggac
     1801 cggtcgccca tcgatggggc tcgcgccaag gagcggctcg aagacctgcc cgagcaggtt
     1861 gatcgtcgtc gaatcgctgg ccaggagcgg gttggtcgtg ctgatatcgc cgttcgaccc
     1921 gaggacgacg gtgccgccct gcttctgcgg cgcgtccatc gggaacgccg acaacagcgc
     1981 gcttttgaac tccgtgcggg tcatggtcga gccgccggcg gtcaatgcga ccggcgagcc
     2041 gaccggcgac gccatgggag acgcgatggg cgatccgacg ggtgaggcaa gcggcgatgc
     2101 ggccgggctg gcgatgggcg aggctacgga cgtcggcgtg attgcccggg tcggcatcgg
     2161 cgccggcgtc gaggtcggct ccttggacgt cgaactgcct ccgcacgcgc cgacgaggat
     2221 agcgatcagc agcaaagcga gcagcgtgct ccagggggat atgcgaattg tgcgcaaagt
     2281 cgttccttgc ccagtcggcg caaacggatc aattcaacct gtcaggggaa ctgtaccgga
     2341 gtcggagtcc cgatccgaaa tcgagccgat tccagccggt gctggcagag cattatgtta
     2401 gacttcggca ctgctggatc aacacgcttc agggtcgaaa accattcacg caggtcacga
     2461 ctttagcatg ctcggaatgc agcgaaactg ctccgagtct cgatctatgc gcccgctcaa
     2521 cgctatgcgc gaggtagcaa ccgcatgatt gtcggcatcc ccagggaaat caaggatcgc
     2581 gagaatcggg tctccacgac gcctgccggg gtcggggagt acgtggctcg cggccacacc
     2641 gtgcttgtcg aatgcgatgc cggcgtcggc agcgggttcg ccaataacga gtacgagcgc
     2701 gcgggcgcga cgatggtcac cgcccacgac gccgtcttcg cccaggcgga catgatcgtc
     2761 aaggtcaaag agccgatccc gagcgagtac gacctgctcc gcgagaatca actgctgttc
     2821 acctatctgc acctggccgc cgaagaggag ctgacgcacg cgctgatgcg gcgcaaggtg
     2881 aaggccgtcg cgtatgagac agtccagctc ggcaccggct cgctcccgct gctgacgccg
     2941 atgagcgaga ttgccggccg gatgtcgatc caggtcgggg cccactatct cgagcggacc
     3001 tacggtggtg agggcctgct gctcggcggc gtcgcgggtg tgccgggcgc ggaggtcgtg
     3061 gtcgtcggtg gcggcgtggt cggcaccaat gccgcgcaga tggcgctcgg aatgggcgcc
     3121 aacgtgacga tcatcgacaa gaacgtggac aggttgcgct acctcgatca ggtgctgcac
     3181 ggccgctgcc acacgctggc ctcgaatgcg cggaatctgc acgacaagat cgcgctggcc
     3241 gacttcgttg tcggcggcgt gctcctgccg ggcgcgaagg cgccgaagct ggtcacacgc
     3301 gagatgattg agagcatgaa gccgggatcg gtcgtggtcg atgtggcgat cgaccagggc
     3361 ggctgcttcg agacttcgaa gccgaccacc catagcgatc ctgtctatga cgtcaaaggc
     3421 gttattcact attgcgtgac aaatatgccg ggcgcggtgg ctcgaacgag tacactcgcg
     3481 ctttcgaatg tgaccctgcc gtatgggctg gcgttggccg acatgggcct cgttgatgcg
     3541 gcacgggcat ttccggaact tgcgaagggc atcaatgtcc tgaacggcgt cgtgacctat
     3601 aaggccgtcg cggacgcgtt cggcctcccg tccgaaccgt gggacgtagt ggcctaagca
     3661 aatcagaccc tccggacaga ccatcctgtg cttccggggg tgaaggggaa agggatacgt
     3721 ggacgaagat ttgaagtccg ctgttcaaca gtttttgacc gcgctggagg cggaccgcgg
     3781 tttcgcggta aatacgatcg cggcctatcg caatgacctg gagcagttct gcgcctatgt
     3841 gcagaacccg ccccaggaag accatatccc ggcgatcgcg cagtggagcg agttgacgga
     3901 ggggcatctc agcacctaca tcctccacct ccgcggccgg gagtatgcgg cgtcgacgat
     3961 cgcccgcaag acggcggcac tcaagtcgtt cagcgcccac ctcgtcgacc ggggcgtgac
     4021 ccgggtcgac gcggccgaga agctgagcgc gccgcgggtc gagaaataca cgccccgctc
     4081 gatcagcccg gccgagatcg accaactgct ggcccaaacc gcgcgcgccg cggccgagaa
     4141 ccggccggaa gggctgcgcg accaggcgat gctcgagacg ctctattcaa cggggatgcg
     4201 cgtcagcgag ctggttgcgc tcaacctgcc cgacatcgac ctcgagcgcc gggtggcgaa
     4261 gtgtaccggc aagggcggcc ggcagcggtc ggtgccgctg cggaccagcg cgatcaacgc
     4321 gctgcgcaac tacctcgagc atggccggcc ccggatcgcg ccgaacgagg agcaggcgct
     4381 tttcgtcaat catcgcggca gccggctgac ccggcagggc ttctggctga tcctcaaggc
     4441 ctacgcgcgc caggcgggga tccaggacat tacgccgcac accctgcgcc attcgttcgc
     4501 gacccatgcg ctgcgccacg ggtacgagct gcgtgatgtg cagcagatgc tcggccatgt
     4561 cagcatttcg accacgcagg tctaccgccg catcgcagtg ccgacccctg ccgtggaacc
     4621 ggccgcctca ggcgaataac taattcggag aaacgctcag cagatgagtc agtcggtacg
     4681 tgctgcgtca atggacaaga tcgtcgcgct cgccaagcgg cgcgggttcg tcttcccggg
     4741 ctccgatatc tacggcggcc tggccaatac ctgggattac gggccgctcg gcaccgagct
     4801 caagaacaac gtcaagcagc tctggtggcg aaccttcgtg cagcttcggc gcgacatggt
     4861 tgggcttgat gccggcatcc tgatgcatcc gcgcgtctgg gaggcctccg gccacgtcgt
     4921 caacttcaac gacccgctgg tcgactgcaa gacctgcaag agccggttcc gggccgacca
     4981 cctgatcgag gaaaagctgg gtattcacgc cgagggc
//
LOCUS       DASSGX010000046         3911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_8477, whole genome shotgun sequence.
ACCESSION   DASSGX010000046 DASSGX010000000
VERSION     DASSGX010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8477"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="VFI12_00700"
     CDS             <1..608
                     /locus_tag="VFI12_00700"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HET7054943.1"
                     /translation="STGLHGQWRHAALDRLPGEGSRAVPQNIELKVGSSAAQFAAIRD
                     RLSQRTCCSCETLIQVDTYFRVPYGRLKLREIESTIGKTAELIQYDRPDRAGVRTSTY
                     QRVDVNPEVKLALLAALGELVTVRKRRTVAIWHSTRIHLDEVDGLGLFIELETVLDEG
                     ERSTERGRAEYDDVVEWLDLASLEPIPGSYSDLLIEKGQHA"
     gene            605..1528
                     /locus_tag="VFI12_00705"
     CDS             605..1528
                     /locus_tag="VFI12_00705"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET7054944.1"
                     /translation="MKVGVIIPLHAPQTGPVVTAPTYAEIMAVAQTAEREGLDSAWIT
                     DHLLFRMPPDYVTRASHEAFTIWAGLAEATSRIELGALVLCTAFRNPAIMTKEAISLD
                     AVANHRVILGLGCGWHEPEFKAFGIPFTHRVSMFEEAMSIIHPLLRQGEVTFDGRFYR
                     AEDCVLAPPDDRPGGIPIMIASKQPRMMELTARFADQWNTAWWGDVDGYLEAAAKQDA
                     ACEKIGRDPATLVKTAGVMIDFMNDVPPADSYDPARIVKGPVEHVAGKLRAYADAGCA
                     HVIGFLVGLTPEKTAMFAEAARKAGLVGANA"
     gene            complement(1967..2221)
                     /locus_tag="VFI12_00710"
     CDS             complement(1967..2221)
                     /locus_tag="VFI12_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7054945.1"
                     /translation="MSDVSMDQPGIPVQTAHLAHSEDERPPLCPTCNKRLVILATQSA
                     RAESGEYIRQQLWGCPRGHATAVRRHGSFLPVEILAELVG"
     gene            complement(2500..3531)
                     /locus_tag="VFI12_00715"
     CDS             complement(2500..3531)
                     /locus_tag="VFI12_00715"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7054946.1"
                     /translation="MELALYRITRRGLIKVGAGTAGLAVLGSRLGQTSAQESYKITLI
                     QGVAGDEFYVSMGCGAQAAATETGSQLTIQGTEKWDATLQIPLLNSVVQSKPDAILIA
                     PNDRTALIQPIQDALNAGIAVFTVDTLIEADIAIANIASDNVQGGKMAAQQLAKLINE
                     TGSVYLQNTVPGASTTDQRQQGFEDEIKNFPNIKYLGVDFNNDDPTTAASQTAAKLQA
                     NPDLAGIFGTNLFSAEGAATAVKEAGKQGQIKIVGFDAGPKQVQDLKEGVVDVLIGQH
                     PYDIGYKGVMMAVEYLKTKQAPTEKTVATGYTVVTKDNLDDPDVKKYLYVADCSEIPA
                     STPEASPTS"
     gene            complement(3644..>3911)
                     /locus_tag="VFI12_00720"
     CDS             complement(3644..>3911)
                     /locus_tag="VFI12_00720"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7054947.1"
                     /translation="ARFGTANASTHSTDNLNAISAVVIGGTSLFGGMGSIIGTVIGAF
                     IPATLRNGLVIGGINPFYQQVLVGGILLLAVYFDQRRRKAEERM"
BASE COUNT          658 a         1233 c         1297 g          723 t
ORIGIN      
        1 actcgaccgg cctccacggt cagtggcgtc acgcagcgtt ggatagactg ccgggggagg
       61 gaagtcgggc agtgccgcag aacatcgagc tcaaggtcgg gtccagcgcc gcgcaattcg
      121 ccgccatccg cgaccggctg agtcagcgaa cctgttgctc ctgcgagacc ctcatccagg
      181 tcgataccta tttccgggta ccgtacggcc ggctaaagct gcgcgaaatc gagtccacca
      241 tcggcaagac cgccgagctg atccagtacg accggccgga ccgtgccgga gtgcgtacca
      301 gcacgtacca gcgcgttgat gtcaaccccg aggtgaagct ggcgctcctg gcggctctcg
      361 gcgagctcgt gaccgtgcga aagcggcgga ccgtcgcgat ctggcattcc acccggattc
      421 atctcgacga ggtcgatggc ctcgggctgt tcatcgagct ggagacagtc ctggacgaag
      481 gtgaacgcag caccgagcgc ggccgggcgg aatacgacga cgtggtcgag tggctcgatc
      541 ttgcgagcct ggagccaatc cccggctcat acagcgattt gttgatcgag aaaggacagc
      601 atgcatgaaa gtcggcgtca tcatccccct tcacgccccg cagaccgggc cggtcgtgac
      661 cgcgcccacc tacgcggaga tcatggcagt cgcgcagaca gccgagcggg aggggttgga
      721 ttcggcctgg atcacggacc atctcctctt ccggatgccg ccggattacg tgacccgcgc
      781 cagccatgaa gccttcacga tctgggccgg tttggcggag gcaaccagcc ggatcgagct
      841 gggcgccctc gtcctttgca ccgccttccg caacccggcg atcatgacca aggaggccat
      901 ctcgctcgac gccgtggcta accaccgggt catactcggc ctcggttgcg ggtggcacga
      961 gccggagttc aaggcgttcg gcatcccctt cacgcaccgc gtcagcatgt tcgaggaggc
     1021 gatgtcgatc atccacccgc tgttgcggca aggcgaggtg accttcgatg gccggttcta
     1081 ccgggccgag gattgcgtcc tggcgccgcc ggatgaccgg ccgggcggga tcccgatcat
     1141 gatcgcgtca aagcagcccc ggatgatgga gctgaccgcc cgctttgccg accagtggaa
     1201 cacggcgtgg tggggcgacg tcgacggcta tctcgaggcc gccgcgaagc aggacgccgc
     1261 ctgcgagaag attggacgcg acccggccac gctggtcaag acggccggcg tcatgatcga
     1321 tttcatgaac gacgtgccgc cggccgactc ctacgatccg gcccggatcg tcaaggggcc
     1381 ggtcgagcac gtcgccggga agctgcgagc gtacgcggac gccgggtgcg cccacgtcat
     1441 cggcttcctg gtcggcctga ctcccgagaa gaccgcaatg ttcgccgaag cggcccgcaa
     1501 agccggcctg gtcggtgcca acgcgtgaac cgccaggatg gcgcagctgg gggcctggtc
     1561 cgactccgga atccggacga tcggcatcgg ccggagctaa aggtccgggc tgaaccacga
     1621 aaagcccatt agaggagctg aacgggcgtg ccgatttcga agaggattgt cgacggctcc
     1681 ccgggctgaa ggccggggtt gagcaacaat gagtcggctg acgccgactc gcgtcgaacg
     1741 cgcctatttc agtcccttca gtggacttgt tgtcatgtcg cccgggccgt tagccccggg
     1801 cggcaccgga tggccggttg gaaattcgga cacatttccg ggagcgatca ccacctccgg
     1861 gcgattgctg gactgccgcg ctgtgccacg gattgatgcc ggatagcaaa cgagcccggc
     1921 agatcgatct gccgggctcc tccttgattt cgatttcgga ttggtatcag ccaacgagct
     1981 ccgcgaggat ctcgacgggc aggaatgagc cgtgacgacg gacggcggtc gcgtggccac
     2041 ggggacatcc ccagagctgc tgacggatgt attcgccgct ctcggcccgc gcggattgcg
     2101 tggcgaggat cacaagccgc ttattgcatg tcgggcacag cggtggccgc tcatcctcgc
     2161 tgtgggcaag gtgcgcggtt tgcacgggga ttcctggttg gtccattgac acgtcgctca
     2221 ctggaagcgg ctttccttct gtctacgctc gcatgacggg agggcgcgtg tttcttcgcg
     2281 ttcgcgtcga ctacccgccg gggcgcgttg ccgttgctgg tttatcctgc tggcatcgcc
     2341 cggtttcggc aatgcgtccc taccgccagt tcgtcccgtc ccaaatcggg aaggtatcga
     2401 gccgtggcac gacgttcggg cgatgtatac cgcgctacga atacggggcg gccttccggt
     2461 aaggaagacc gccccgtagt gcaggcgaat cgccgcgact tatgaggtcg gcgaagcttc
     2521 cggcgtgctg gccgggatct ccgagcagtc ggcgacgtag aggtacttct tgacgtccgg
     2581 atcgtccagg ttgtccttgg tcacgacggt gtagccggtc gcgacggtct tctccgtcgg
     2641 cgcctgcttg gtcttcaggt actcgaccgc catcatgacg cccttgtagc cgatgtcgta
     2701 cgggtgctgg ccgatgagga cgtcgacgac gccttccttc aggtcctgga cctgcttcgg
     2761 gccggcatcg aagccgacga tcttgatctg gccctgcttg cccgcttcct tgacggccgt
     2821 ggctgcgccc tcggcgctga acaggttggt gccgaagatg ccggccagat ccgggttggc
     2881 ctggagcttg gccgcggtct gcgaggcagc cgtcgtcggg tcgtcgttgt tgaagtcgac
     2941 gccgaggtac ttgatgttcg ggaagttctt gatctcgtcc tcgaagccct gctgccgctg
     3001 gtcggtcgtc gaggcgcccg ggaccgtgtt ctggaggtag acagagccgg tctcgttgat
     3061 cagcttggcg agctgctgcg ccgccatctt cccgccctgg acattgtctg aggcgatgtt
     3121 ggcgattgcg atgtcggcct cgatcagcgt gtcgaccgtg aagacggcga tgcctgcgtt
     3181 gagcgcgtcc tggatcggct ggatcagggc ggtgcggtcg ttcggggcaa tcaggatcgc
     3241 gtccggcttg gactggacga ccgagttgag gagcgggatc tggagcgtgg catcccactt
     3301 ctccgtgccc tggatcgtca gctgggagcc ggtctcagta gcggctgcct gcgcgccgca
     3361 tcccatcgag acatagaact cgtcgccggc gacaccctgg atcagggtga tcttatagct
     3421 ctcctgggcc gaagtctggc ccaaccggct gcccaaaacg gccaacccgg cggtgcctgc
     3481 tccaaccttg atcaagccac gtcgcgtgat tcgatacaac gccaattcca cggctcactc
     3541 tcctcgtcga tcctggtgcg cggggcgcac ccaaatccgt gccagcacaa atgcctggaa
     3601 tgtcatcgta gcaagaacgt agtttcggct gcaatggagg ctgctacatc cgctcctcag
     3661 ccttgcgccg gcgctggtcg aaatacacgg ccaggagcag aatcccgccg accaggacct
     3721 gctggtaaaa cgggttgatc ccgccgatca ccaggccatt ccggagcgtg gcggggatga
     3781 acgcgccgat caccgtgccg ataatcgagc ccatcccgcc gaagaggctt gtcccgccga
     3841 tcacgaccgc cgagatcgcg ttcaggttgt cggtggagtg cgtcgaggca ttggcggtgc
     3901 cgaaccgggc g
//
LOCUS       DASSGX010000047         2988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1532916, whole genome shotgun sequence.
ACCESSION   DASSGX010000047 DASSGX010000000
VERSION     DASSGX010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1532916"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(211..750)
                     /locus_tag="VFI12_00725"
     CDS             complement(211..750)
                     /locus_tag="VFI12_00725"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7054948.1"
                     /translation="MSEPIFETERLNVRRYEPGDAPAMFEVYRNPDVWRYLAGGEPYA
                     SVEQTRERLEQHIQWYEDDTPYGLWAVVVRDTGELIGSVLLALLEGGPEVEVGYHFGT
                     FAWGNGYATEVTRGAIRYGFDVLGLQQICGVVFAENVASQHVLEKAGLAYRGLRSVFG
                     FDGVMYYTIDRPDPGNRPG"
     gene            complement(859..1713)
                     /locus_tag="VFI12_00730"
     CDS             complement(859..1713)
                     /locus_tag="VFI12_00730"
                     /inference="COORDINATES: protein motif:HMM:NF021098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC and DUF2382 domain-containing protein"
                     /protein_id="HET7054949.1"
                     /translation="METPMTQYQFRAGDDVIAADGDKVGSLADIHGNYLVVEKGFFFP
                     RDYYIPFNSVANYDPGDGKIYLSVTKDAALNSGWDQKPDFTDETYATGTTAATGAYAG
                     DTTREAFETDRAAAGASGVTGGMYGNDLTETTRDMATNRTTELTDKDHIRVPVHEEEL
                     IATKTPRQAGQVRVSKDVVEEERAIDVPVTEERVRVTRRAVDQAADTGDVAFQEESFE
                     VPVRTEDVGVDKVVRVAEEIDIDKETVQDTKRVSGTVRKERVNVDESGTDASIYADKG
                     KIDDTAST"
     gene            1933..>2988
                     /locus_tag="VFI12_00735"
     CDS             1933..>2988
                     /locus_tag="VFI12_00735"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7054950.1"
                     /translation="MWNPKESFADAEPVVYWTSQSRPVPVQPPLASDGVADLVVVGGG
                     FTGLWAAIEAKQRDPGRDVVLLEMERVAFGASGRNGGFIEPSITHGLENGLARYSPEE
                     MQELIQLGNENYAEIAEFIRAHQIDCDLQEHGVVWAATEPHLAEAIPEIARLHTEWGQ
                     RTDALTAGELRAEVNSPFYLGGVWMRDEGGLVDPARLAWGLLRVALDLGVRVHEGTRV
                     SELVDAGSHVAVRTTGGTLLARKVLHATSAYRGVIPEIRRYVVPVYDYVLMSEPLSEA
                     QRAAIGWRTRFGLSDGDNQFVYHRMTADGRILYGGWDAVYHRGGRVHPSLDQRDASHE
                     FLAERFFRTFPQLEGLRF"
BASE COUNT          509 a          900 c         1019 g          560 t
ORIGIN      
        1 agggaatcct cggcgacggg agtgccggcg atcgcggctg ttccgaatcg caaaccggtg
       61 atcggcaagc ctgcaagact gacggtcccg gccagaaatg cgcgtcgatg catggtagtc
      121 gctcctgctc gtcaacgaat acgacacgaa cgacgatatg gggcggactt tcccggcgcg
      181 agaggcagga ttcccgattg ggccgggaac ttatcccggg cgattaccgg gatcggggcg
      241 atcgatggtg tagtacatga cgccgtcgaa gccgaagacc gaacggaggc cacgatacgc
      301 caggccggct ttttcgagga cgtgctgcga ggcgacgttt tccgcgaaga cgacgccgca
      361 gatttgttgc agcccgagga cgtcgaaacc gtagcggatc gcgccccgcg tgacctcggt
      421 ggcgtagcca ttgccccacg cgaacgtgcc aaagtggtag ccgacctcga cctcggggcc
      481 gccttcgagc agagccagca ggacggagcc gatcagttcg ccggtgtcgc gtacgacaac
      541 agcccagagc ccatatgggg tgtcatcctc gtaccattgg atatgctgct ccaggcgctc
      601 gcgggtctgt tccaccgacg cgtacggctc gccgccggca aggtatcgcc agacgtccgg
      661 attccggtag acctcgaaca tggctggagc gtcgcccggc tcataccggc ggacgttcag
      721 ccgctccgtt tcgaagattg gctcgctcat gcattcccgc tccctgaacg ggaggaacaa
      781 tatgcgattg gcgatcacaa cacgaaaggc ggcgccggat caccggcgcc gccgttctga
      841 aggtgaggaa gggaaggtct acgtgctggc ggtatcgtcg atcttgccct tgtcggcgta
      901 gatcgaggcg tcggttccgg attcgtcgac attgacccgc tccttgcgga ccgtgccgga
      961 gacacgcttg gtgtcctgga cggtttcctt gtcgatgtcg atctcttcgg ccacgcgcac
     1021 gaccttgtcg acgccgacgt cctccgtgcg gaccggtact tcgaagctct cttcctggaa
     1081 agcaacgtcg ccggtgtcgg ccgcctggtc gacagcgcgg cgcgtgacgc ggacgcgctc
     1141 ctcggtgacc ggaacgtcaa ttgcgcgctc ctcttcgacc acgtccttgg atacgcggac
     1201 ctggccggcc tggcgcgggg tcttggtggc gatcaactct tcctcgtgga ccggcacgcg
     1261 gatgtggtcc ttatcggtga gctcggtcgt gcgattggtc gccatgtcgc gggtggtttc
     1321 ggtcaggtca ttgccataca tgccgcccgt cacgccggac gctccggcgg cggcgcggtc
     1381 ggtttcgaac gcctcgcggg tcgtgtcgcc agcgtaggcg ccggtggccg cagttgtgcc
     1441 ggtggcatac gtctcatcgg tgaagtccgg cttctggtcc cagccactgt tgagggccgc
     1501 atccttcgtc accgagagat agatcttgcc gtcacccgga tcgtagttgg cgacgctgtt
     1561 gaacgggatg tagtagtcgc gcgggaagaa aaagcccttc tccacgacca ggtaatttcc
     1621 gtgaatgtca gccaggcttc cgaccttatc gccgtcagcg gcgatgacat cgtcaccggc
     1681 gcggaactgg tattgggtca tcggggtttc cataaatata gcctccttcg aagtcccgtt
     1741 catggacatg ccacgaacgt agggtaggac cttagcaccg tggttcggag aagcactgtg
     1801 actggactac cctgatcggc ccgcagccgg cgcccggcgt agccttggtg acggtcctga
     1861 acgccatcgc gcttgactgc gcggcaaacg tgccggtcaa cgaccggaag cggcgtcgga
     1921 taccatgccg ccatgtggaa cccgaaggag tcattcgccg atgccgagcc ggtcgtgtac
     1981 tggacgagcc agtcgcgccc tgtgccggtg cagccgccat tagcatcgga tggggtggcg
     2041 gatctcgtcg tcgtcggtgg cggcttcacc gggttgtggg cggcaatcga ggcgaagcag
     2101 cgggatccgg gtcgcgacgt cgtgttgctc gagatggagc gggtcgcatt cggggcgagt
     2161 ggccggaatg gcggctttat cgagccatcg atcacgcacg gcctcgagaa cggacttgca
     2221 cggtactcgc cggaagagat gcaggagctg atccagctcg ggaacgagaa ctacgccgaa
     2281 atcgccgagt tcatccgcgc gcaccagatc gactgcgatt tacaggagca tggagttgtg
     2341 tgggcggcga ccgagccgca cctcgccgag gcaatccccg agatcgccag gctgcacacc
     2401 gaatgggggc aacgaaccga cgccttgacc gccggggaac tgcgggcaga ggtcaattcg
     2461 ccgttctacc tcggcggtgt ctggatgcgg gacgagggcg gtctggtcga tccggcgcga
     2521 ctggcgtggg gattgttgcg agtggcgctg gatctgggcg tgcgggtcca tgaggggacg
     2581 cgggtctcgg aactggtcga tgccggcagc catgttgcgg tccggacaac cggtggcacg
     2641 ctgctcgcgc gaaaggtgtt gcacgcgacc agcgcctacc ggggtgtgat tccggagatc
     2701 cggcgatacg tcgtgccggt ttacgactac gtgttgatgt ccgagccgct gtcggaggcg
     2761 caacgggcgg cgatcggctg gcggacgcgg ttcggcctga gtgacggcga caaccagttc
     2821 gtctaccacc ggatgacggc ggacggccgc atcctctatg gcggatggga tgcggtctat
     2881 caccggggcg gccgagtgca tccatcgctc gaccagcggg atgcctcgca tgaatttctc
     2941 gccgagcgct tcttccggac cttcccgcag ctcgaagggt tgcggttc
//
LOCUS       DASSGX010000048         2890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_552652, whole genome shotgun sequence.
ACCESSION   DASSGX010000048 DASSGX010000000
VERSION     DASSGX010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552652"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..1183
                     /locus_tag="VFI12_00740"
     CDS             92..1183
                     /locus_tag="VFI12_00740"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF003645.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HET7054951.1"
                     /translation="MIDLAYRAAKPLLFRIDPERVHDRVIAALGLISRSPGALRILEH
                     FGEPDDPRLMVEIAGIRLPGPIGIAAGLDKNAVAYPALHALGWDFIEVGTITLKPQPG
                     NPKPRVFRLAADHALINRMGFPGQGADAIAMHLVERRAAGVPIGCNIGPNKASVESGL
                     DAVIADCRLLASRFASLATYLVVNISSPNTARLRELQGKEALRELMSEVKAAIPVDRP
                     TPLFVKIAPDLTDAEISDVVGVVQEVGVSGIVATNTTIARPDSLAGPAKTESGGLSGA
                     PLTKRSLAVVGQIVRESGGSVPIIAAGGIASGADAIAALRAGASAVQIYTGMIYEGPG
                     LARRIKCEIVTEMDRIGAPSLADLRVAGA"
     gene            complement(1122..1988)
                     /gene="fdhD"
                     /locus_tag="VFI12_00745"
     CDS             complement(1122..1988)
                     /gene="fdhD"
                     /locus_tag="VFI12_00745"
                     /inference="COORDINATES: protein motif:HMM:NF014671.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HET7054952.1"
                     /translation="MRVTAVAGDRSRQRSDWLATEEPLEIRVTDATTLDPVLTHVTMR
                     TPGADFELAAGFLLAEGIVTRFEQIRQMEFCVGPAATAIQQYNIVSVEVTDPDLTNRS
                     EAHIRTAVSACGVCGKASLDELSLRGVEPVSSDVQISQEVIEKLPQLLRERQALFEST
                     GGLHGAALITVSGDVLAVREDVGRHNAVDKLLGWALLNPKPAEDRAILMVSGRLSYEL
                     AQKAAVGRIPVLAGVSAPSSLAVELAREFGLTLIGFLRGDSFNIYAGQGRIRLRQPAD
                     LQATERRSGPSR"
     gene            complement(2020..>2890)
                     /locus_tag="VFI12_00750"
     CDS             complement(2020..>2890)
                     /locus_tag="VFI12_00750"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HET7054953.1"
                     /translation="VLVAMGGNFREAMPDPGYVDRALSSIPLRVHIDIMLSNQMLVDP
                     ADTVVVLPAMTRYEIPGGITQTSTERRIMFSPEIEGPRIGEARPEWQLFTELAGRVRP
                     DLAERVAFSGTQAIREEIARVVPAYAGIETLRKTGDQVQYGGPHLAAGWNFPTANGRG
                     SFSVVDLPDAAIPEGTFALATRRGKQFNTMVHEKSDAMTGAASRDAVFMHEADARALG
                     LADGDPVMLRNEFGEMTGRVYFSRIQPRNVQVYWPEGNVLLDHRRRSPQSHVPHYNAF
                     VTIEPAAVAHAAD"
BASE COUNT          476 a         1005 c          914 g          495 t
ORIGIN      
        1 gatgttgcgg cgttcctcta cagctggacc gatatcggca ctgccgtcac cgtcatcccg
       61 tagggcaatc acgccgtggt atcgtcacgg cgtgatcgac ctcgcctacc gcgccgccaa
      121 accgctgctc tttcggatcg atcccgaacg agtccacgac cgcgtcattg ccgccctcgg
      181 cctgatcagc agatcgccgg gggcacttcg catcctcgag catttcggcg agccggacga
      241 cccccggctc atggtcgaga ttgccgggat acgactcccc ggaccgatcg ggatcgctgc
      301 cggcctggac aagaacgcgg tcgcctaccc cgccctgcac gcgctcggct gggatttcat
      361 cgaagtcggc actatcacgc tgaaaccgca accgggcaat ccgaagccgc gcgtctttcg
      421 cctcgcggcc gaccacgcgc tgatcaaccg catgggcttc cccgggcagg gcgcggatgc
      481 gatcgcgatg cacctggtcg agcggcgagc cgccggtgtt ccgatcggct gcaacatcgg
      541 gccgaacaag gcgagcgtcg agtccgggct ggacgcggtg attgccgatt gccggctgct
      601 ggcgagccgc ttcgcctccc ttgccaccta cctcgtcgtc aacatctcgt cgcccaatac
      661 ggcccggttg cgcgaattgc agggcaaaga ggcgctccgg gagctgatgt ctgaggtcaa
      721 ggcggcaatc cctgtcgatc ggccgacgcc gctcttcgtc aagattgcgc ccgacctgac
      781 cgatgccgag atcagcgatg tcgtgggtgt cgtccaggag gtcggcgtca gtggcatcgt
      841 ggcgacaaac acgactatcg cgcggccgga ttcgcttgcg ggtcccgcaa agacagagtc
      901 cggcggcctg agcggcgccc cgctgacaaa gcggtcgctt gcggtggtgg gtcagatcgt
      961 ccgcgagtcg ggaggctccg tgccgatcat cgcggccggc gggatcgctt ccggagccga
     1021 cgcgatcgct gcgctccggg ccggggcctc ggccgtacag atatacacgg gcatgatcta
     1081 cgagggtcca ggactggccc ggagaatcaa gtgcgagatc gtcaccgaga tggaccggat
     1141 cggcgctccg tcgcttgcag atctgcgggt tgccggagcc tgatcctccc ctgccccgcg
     1201 tagatgttga atgagtcgcc gcgcaagaag ccaatcagtg tcagtccaaa ttctcgggcc
     1261 aactctacgg ccaggctgct cggcgcggag acaccggcca gcaccgggat ccggccgacc
     1321 gccgccttct gcgccagttc gtagctcagc cgcccactca ccatgaggat cgcccggtct
     1381 tccgccggct tcggattcag cagcgcccat ccgagcagct tgtcgaccgc attatgtcgc
     1441 cccacgtcct cacgaacggc cagcacgtcg cccgataccg tgatcagcgc cgcgccatgt
     1501 aacccaccgg tactctcgaa tagcgcctgc cgttcccgga gcaactgggg caacttttca
     1561 atcacctcct ggctgatctg cacatcgctc gagaccggct cgacgccgcg cagcgacaat
     1621 tcgtcgagcg aggctttgcc gcagacgcca caggcactca cggcggtccg gatatgggct
     1681 tcggaccgat tagtaaggtc cggatcagtt acctccacac tgacgatgtt gtactgctgg
     1741 atcgcggtcg cagccggacc gacgcagaat tccatctgcc ggatctgctc gaatcgcgtg
     1801 acgatcccct cagccagcag gaatcccgcc gccagttcga aatccgctcc cggcgttcgc
     1861 atcgtgacgt gcgtcaggac cgggtcgagc gtcgtggcgt cggtcacccg gatctcgagc
     1921 ggctcctcgg ttgcgagcca gtccgagcgc tgccgggagc gatcgccggc caccgcggtg
     1981 acgcgcatcc gcgtccggct cgcctttcgc ccgtccacgt cagtcggccg cgtgggcgac
     2041 cgccgccggc tcgattgtca cgaaggcgtt gtaatgcggc acatgcgatt ggggcgagcg
     2101 tcgccggtgg tccagcagca cgttgccttc cggccagtag acctgcacgt tccgcggctg
     2161 gattcgcgag aagtagaccc ggccagtcat ctccccgaat tcgttgcgca gcatgaccgg
     2221 atcgccgtcc gccagcccga gcgcccgggc atccgcctcg tgcatgaaga cggcgtcccg
     2281 ggacgccgcg ccggtcatcg cgtctgattt ctcgtggacc atcgtattga actgcttgcc
     2341 tcgccgcgtg gcgagcgcga acgtgccctc gggaatcgcg gcatccggga ggtcaacgac
     2401 gctgaacgag ccgcgcccgt tggcggtcgg gaagttccac ccggcggcga ggtgcggccc
     2461 gccgtactgg acctgatcgc cggtcttccg gagcgtctcg ataccggcat acgccggcac
     2521 gacgcgcgcg atctcctcgc gaatcgcctg tgttccggag aacgcgaccc gttcggccaa
     2581 gtccggccgg actcgccccg ccagctcggt gaagagctgc cactccggcc gcgcctcgcc
     2641 gatgcgcgga ccctcgatct ccgggctaaa catgatccgt cgctcggtcg aggtctgggt
     2701 gatcccgccc ggaatctcat accgggtcat tgccggcagc accacgaccg tgtcggccgg
     2761 atcgaccagc atctggttcg agagcatgat gtcgatgtga acgcgcagcg ggatgctcga
     2821 aagggcacgg tccacatatc cgggatcggg catcgcctca cggaaattgc cgcccatcgc
     2881 gaccaggaca
//
LOCUS       DASSGX010000049         2284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_661847, whole genome shotgun sequence.
ACCESSION   DASSGX010000049 DASSGX010000000
VERSION     DASSGX010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661847"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="VFI12_00755"
     CDS             complement(<1..713)
                     /locus_tag="VFI12_00755"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HET7054954.1"
                     /translation="MVCASETVSNLVWLDRGLASCADVGGKGASLSRLAALGAPVPPA
                     FALTTHAYAQFAASLGLPCRASEIADGELPRLRAMIADAAIPAPILAAITDGYHTLRA
                     LGGPEVAFAVRSSATAEDSEAFSFAGLHDTVLDVRTLPALVTAVKQCWASLWSERAVA
                     YRRAGGLATDDAAIAVVVQLMVRSDVSFVVFTADPVHGRDDHLVIDATWGIGEAVVSG
                     LVVPDHIVVGPDGRVVEYV"
     gene            complement(932..1780)
                     /locus_tag="VFI12_00760"
     CDS             complement(932..1780)
                     /locus_tag="VFI12_00760"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7054955.1"
                     /translation="MQPSLSVAEPFSSPGGRGKTSRATITSRPAGFFADLWSVAYRSL
                     RALPREPELLIPSLVIPVFFFIVNLGALQAFAERGIPGLDYRAFQLPTAIVFAVTGVS
                     RAHAVVTDIQSGYFDRLLMSPVRRLPLLLGMMAADFAQIIILSLPVIALGMIVGVRFE
                     TGLPGFLLFLLLSGCWGLAFTGFGYAIALKTGSAGAVNSSFILFFPFAFLTTATLPLE
                     ALTGWMASIARFNPMTYLLAGLRSLLYGGWQPDLLAQAVTAILGVGVVSIGLALAALR
                     SRVTRG"
     gene            complement(1786..>2284)
                     /locus_tag="VFI12_00765"
     CDS             complement(1786..>2284)
                     /locus_tag="VFI12_00765"
                     /inference="COORDINATES: protein motif:HMM:NF025109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4162 domain-containing protein"
                     /protein_id="HET7054956.1"
                     /translation="LIHNPEVLFLDEPTTGLDPASRASIWAEVQRLNQELGMTIFLTT
                     QYLEEADALADRVGIIDHGKIIAEGTPAALKRAIGADVVAVHVDDVARAVPVVAAVPG
                     VDGVETRGEELLAATANGPAAVSPIAVRLAEAGVRVRALTMREPTLDDVFLTLTGQRL
                     EGEAA"
BASE COUNT          416 a          769 c          799 g          300 t
ORIGIN      
        1 atgacgtact cgacgacgcg accatctgga ccgaccacga tgtgatcggg gaccacgagt
       61 cccgacacca ccgcttcgcc gatcccccac gtcgcgtcga tgacgaggtg gtcgtcgcga
      121 ccgtggaccg ggtcggcggt gaagacgacg aaggagacgt cgctccggac cataagctgg
      181 acgacgacag cgattgccgc gtcgtcggtc gctaacccgc cagcccgacg gtacgccacc
      241 gctcgttccg accagaggga cgcccagcac tgtttgaccg cggtcaccag cgccggcagg
      301 gtgcgcacat cgaggacggt gtcgtgcaga ccggcgaagg agaaggcctc cgaatcttcg
      361 gccgtcgccg acgagcggac ggcgaacgcc acctccgggc cgcccagggc tcggagcgtg
      421 tggtagccgt cggtgatcgc cgccaggatc ggggcgggaa tcgccgcgtc ggcaatcatt
      481 gcccgcaggc gaggaagttc tccgtcggcg atttcgctgg cccgacacgg caggccgagc
      541 gacgccgcga actgagcgta ggcgtgggtc gtcaacgcga atgccggcgg gacgggcgcg
      601 ccgagcgcgg cgagccggct caggctggcg cccttgccgc cgacatccgc acagctcgcg
      661 agcccccgat cgagccagac gaggttcgag accgtctccg atgcgcaaac catgcaaacg
      721 tctttctatg atggacgcgg accttgcgca ttttgggacc acggcgaccg aaatgcaatc
      781 ccgcgccgct ctccctctgc cgagaaaggt ttggggtcac ggtggcgctt ggtcgggcca
      841 tgcgctgggg cgagagacga ggcaacaggg cacggcacgg cgcgccgtgc cgtgccctgc
      901 cagattgccc agccggttgc agtcgccggc gctagcctcg cgtcacccgg ctccgcagcg
      961 cggccagggc gagaccgatg ctgacgacgc cgacgcccag gatcgccgtg acagcctgcg
     1021 ccagcaggtc cggctgccag ccgccataga ggagtgagcg aagcccggcc agcagatagg
     1081 tcatggggtt gaagcgggcg atgctcgcca tccacccggt cagcgcctcc agcggcagcg
     1141 tcgccgtggt gaggaaggcg aaggggaaga agaggatgaa actggagttg actgccccgg
     1201 ccgaccccgt cttcagtgca atcgcatagc cgaatccggt gaaggcgagt ccccagcacc
     1261 cggacagcag caggaagagc aggaaccccg gcagaccggt ttcgaagcgg acgccgacga
     1321 tcatgccgag ggcgatgacc gggagcgaca ggatgatgat ctgcgcgaag tcggccgcca
     1381 tcatgccgag cagcagcggc agccgccgca ccggactcat caacaggcga tcgaaatagc
     1441 cggactggat gtcggtcacc accgcatgcg cgcgagagac cccggtcacg gcgaagacga
     1501 tcgcggtcgg gagctggaac gcccggtagt cgagcccggg gataccgcgc tcggcgaacg
     1561 cctgcaaggc gccgagattg acgatgaaga agaagaccgg gatcaccagc gacgggatca
     1621 acagctccgg ctcgcgcggc agcgcccgca gcgaacggta ggcaaccgac cagagatcgg
     1681 cgaagaatcc ggcgggccgg gaggtgatcg tcgccctgga tgtcttcccg cgcccgccgg
     1741 gcgaggaaaa cggctctgcg acagatagcg aaggttgcat cgcactcacg cggcttctcc
     1801 ttccaggcgt tgcccggtaa gcgtcaggaa tacgtcatcg agcgtcggct cgcgcatcgt
     1861 gagggcgcgc acgcgcaccc cggcctcggc cagccggacg gcgatcgggg agaccgcggc
     1921 aggcccgttc gcggtcgccg ccagcagctc ctccccacgg gtctcgacac cgtcgacacc
     1981 ggggacggcc gccaccacgg gtacggcccg cgccacgtcg tcgacatgca cggcgacgac
     2041 atccgcgccg atggcccgtt tcagcgccgc gggcgtcccc tcggcgatga tcttgccgtg
     2101 gtcgatgatc ccgacccggt cggccagggc gtcggcctcc tccaggtact gggtcgtcag
     2161 gaagatcgtc atccccagct cctggttcaa ccgctgcacc tcggcccaga tgctggcgcg
     2221 ggatgccggg tcgagcccgg tcgtcggctc gtcgaggaag aggacttcgg ggttgtggat
     2281 cagg
//
LOCUS       DASSGX010000050         3462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1096602, whole genome shotgun sequence.
ACCESSION   DASSGX010000050 DASSGX010000000
VERSION     DASSGX010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1096602"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="VFI12_00770"
     CDS             <1..792
                     /locus_tag="VFI12_00770"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HET7054957.1"
                     /translation="SGQPFDLILAYNLMDEEWSFRTDRLDGVAPGCTVVALAGKAIAQ
                     TMDIPTIHDGTQIVLAVEPERGPAQRLLSAAGMLGLTGANNDGVGVVVNNLNQLPSSA
                     SGLPVQVLTRRLLEHGTAAEAARWVESIPHAVGQHYLIADSTETISLEGSGAGVDRVP
                     VADNYVHANHPLASAGSRPDTDRIEFLSNTHVRYDRAQSLVDAAADQAGLERILEDRE
                     APISCERKEGFMTFGGISIGLTTPPTMRVTAGPPHESAWIDVSWN"
     gene            896..>3462
                     /locus_tag="VFI12_00775"
     CDS             896..>3462
                     /locus_tag="VFI12_00775"
                     /inference="COORDINATES: protein motif:HMM:NF023260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3352 domain-containing protein"
                     /protein_id="HET7054958.1"
                     /translation="MSIAPRPLTRAWTLAVVLTLMLGLMPLPGGTTSAQDTRTTVLDG
                     SIWDLIPGNATGLIAVNTDTSSEQWRMATDLVGNAGLTQVLTGLFDQVIADAGISPAN
                     FDPAQSSILGGTIAVAAWGDANDPFGSVAVYVDATNPERAYRSIAAQFLTENDPDAIE
                     VEIPGGQLTTDRYGSGAIMLLDGVVVVTEADVAQVIANLVTGAGPTLADFKPFTQVID
                     AQSGDQLIRAYANGPAATRAFAGGFSPYPGYGGLGYQELALFGALAAPVTGTYTSVGI
                     SATDGGFLISSAQLPAPVIGPELPKGRPSDGLVSEVSSTADLFVSGRNLGQNSRFSQI
                     IDMIVISAATSSGAYPDQPTIDLRGWAYDEIERRTGIDLRADLLDQINGDYVMAGALD
                     SVDQLSDVNVVVRTDLDDAETVADTVGKLATLLRVATSDLADGADQLELTESRVGEDT
                     LYTLAAREGGSTVTYSFGVVDSQLLAAMGDGMSLLTESDSGSLADDASYQDAIAKLPE
                     QDGAPFYLDLHDLVTVAGLTFIDAPAGWGLPAGQQIPLGGFQSIAGMTYAKGGLARTD
                     LLLSVTGNAPGSIGTPSSGGSWAPYPGPSNIAIADATAIDVGGRRIHSVAPDGNHAVV
                     RGEGKGETCVVALDGSSAETCTDEIAGIDVKHIAWSPDGTLVAVTENAAQYGVDSDIW
                     IIDAGSGDVRNLTDDDYTGAMPLDIGKADVPDPLQVDVSPIFTPDGAAVVFARSNWGT
                     AEMPDTDLYRVSIDGGEPERITPFGNELFTAWYGAIWVEDSTLLISYGSGDRDEPKNG
                     LWTVDVDSGHLEQLWHGKEQQYVVSPVAQRADGSVVVVFPISLSQDPISSDVCSAAVL
                     "
BASE COUNT          594 a         1133 c         1126 g          609 t
ORIGIN      
        1 tcgggccagc cgttcgacct gatccttgcc tacaacctga tggacgagga gtggagcttc
       61 cggacggatc ggctcgatgg cgtcgcgcct ggttgcacgg tggtcgcgct cgccggaaaa
      121 gcgatcgcgc aaaccatgga catcccgacg atccacgacg gcacgcagat tgtgctcgcg
      181 gtcgaaccgg agcgcggccc ggcgcagcgt ttgctctctg ccgccggcat gctcgggctc
      241 accggagcga acaatgacgg cgtcggcgtg gtcgtcaaca acctgaacca gcttccgtcg
      301 tcagcgagtg gactgccggt gcaggtcttg acccgccgct tactggagca tgggaccgcc
      361 gctgaggccg cacgctgggt cgaatcgatc ccgcacgcgg tcggccagca ttacctgatc
      421 gccgattcga ctgagacgat cagtctcgag gggtccggcg ccggcgtaga tcgggtcccg
      481 gtggcagaca actatgtcca tgccaatcat ccgctcgcga gcgcaggcag ccggccggat
      541 acggaccgga tcgagttcct ctcgaacacg cacgttcgct acgaccgcgc gcagtcgttg
      601 gtcgacgcgg cagcggacca ggcggggctc gagcggatcc tcgaggatcg cgaagcgccg
      661 atcagctgcg agcgcaagga aggcttcatg accttcggcg gcatcagtat cggcctgaca
      721 acgccgccga cgatgcgcgt cacggcgggt cccccgcatg aatcggcctg gatcgacgtc
      781 tcctggaatt gacattcccg ggttccaccg acaggatgga actcttgcta acgcatgagc
      841 gtttcaggcg tgagcgcgat gctcacgcgt ctgccgacat gctccggagg acccgatgtc
      901 gatcgcacct cgcccgctga ctcgcgcctg gacgctcgcc gtcgtcctca cgctcatgct
      961 cggcctgatg cccctgccag gaggtacgac gtccgcgcaa gatacccgga cgaccgtgct
     1021 ggacgggtcg atctgggacc tgattcccgg caacgcgacc ggcctgatcg cggtcaacac
     1081 cgacacctcg tccgagcagt ggcggatggc caccgatctg gtagggaacg ctgggctgac
     1141 acaggtcctg accggcctgt tcgaccaggt catcgccgac gcgggcatct cgccggctaa
     1201 cttcgacccc gcgcaatcct cgatcctcgg cggcacgatc gcggtcgccg cgtggggcga
     1261 tgcgaatgac ccattcggca gtgttgccgt gtacgtcgat gcgactaatc ccgaacgggc
     1321 ataccgatcc atcgccgccc agttcttgac tgagaacgat ccggatgcga tcgaggtcga
     1381 gatcccgggc ggccagctga ccacagacag gtatggatcg ggcgcgatca tgctgcttga
     1441 cggcgtcgtg gttgtcacag aggctgacgt cgcccaggtc atcgccaatc tcgtgaccgg
     1501 cgccggaccg accctcgctg acttcaagcc gttcactcag gtgatcgatg cgcaatccgg
     1561 cgaccagctg atccgcgcct acgcgaatgg tccggccgcg acccgggcgt tcgccggggg
     1621 attctcgccg taccccggtt acggtggcct tggttaccag gaactagccc tgtttggagc
     1681 ccttgcggcg cccgtgaccg ggacctacac cagcgtcggc atttcggcga ccgacggcgg
     1741 cttcctgatc agctccgcgc aacttcctgc cccggtgatc ggaccggagt tgccgaaggg
     1801 acgcccgtcg gatggcctcg tttcggaggt gtccagcacc gccgacctct tcgtcagcgg
     1861 gcgcaatctc ggtcagaatt cgcggtttag ccagatcatc gacatgatcg tgatcagcgc
     1921 ggccacctcg agcggtgcat acccggatca gccgaccatc gacctgcgcg gctgggcgta
     1981 cgatgagatc gagcggcgga ccggcatcga tctgcgcgcc gacctgctcg accagatcaa
     2041 cggcgactac gtgatggccg gcgctctcga ctcggttgat cagctctcgg acgtcaatgt
     2101 cgtggtccga accgacctcg acgatgcgga aactgtggcg gatacggtcg gcaagctggc
     2161 cacgctgctg cgcgtcgcga cctccgacct ggcggacggt gccgaccagc tggaactcac
     2221 cgaatcccgg gtcggcgagg acacgctcta cacgcttgct gcccgggagg gcgggagtac
     2281 cgtcacatac tcgttcgggg tcgttgacag ccaactcctg gcagcgatgg gcgacgggat
     2341 gagcttgctg accgagagcg actccggatc gctggcagac gatgccagct atcaggatgc
     2401 gatcgcaaag ctcccggagc aggacggcgc gcccttctac ctcgacctgc acgacctggt
     2461 cactgtcgcc ggcctcacct tcatcgacgc gccggccggc tggggcctcc ccgccggcca
     2521 gcagatcccg ctcggcgggt tccagtcgat tgccggtatg acctacgcga agggcggtct
     2581 ggcccgcacc gatttgctcc tctcggtcac cgggaatgcg cccggttcaa tcggcacgcc
     2641 atcgtcgggc ggttcgtggg ctccgtatcc cggcccgtcg aatatcgcaa ttgccgacgc
     2701 gacggctatc gatgtcggcg ggcggcggat ccactctgtc gcgcccgacg ggaaccacgc
     2761 ggtcgtcagg ggcgaaggca agggagagac ttgtgtcgtt gctcttgatg gctccagcgc
     2821 tgaaacctgc acggacgaga tcgctgggat cgacgtcaaa cacatcgcct ggtctccgga
     2881 tggcacgttg gtcgccgtca ccgagaacgc ggcccagtac ggggtggaca gcgatatctg
     2941 gatcattgac gccggtagcg gggatgttcg gaacctgaca gacgacgact ataccggcgc
     3001 gatgccgctc gacatcggaa aggcggatgt gccggatccg ctgcaggtcg atgtcagccc
     3061 gatctttacg ccggatggcg cggcggtggt ctttgctcgg tcaaactggg gcaccgccga
     3121 aatgccggac accgacctct accgggtgag cattgacggc ggcgagccgg agcgaatcac
     3181 tccgttcggc aacgagctct tcaccgcctg gtatggtgca atctgggttg aggattcgac
     3241 gctcctgatc tcgtacggtt cgggcgacag ggatgagccc aagaacggcc tctggaccgt
     3301 tgacgtggac tccggccacc tggagcaact ctggcacggt aaggagcagc agtacgtggt
     3361 ctctcctgtc gcgcagcggg cggacggatc ggtggtcgtc gtcttcccga tcagtcttag
     3421 ccaagacccc atatcgtcgg atgtctgctc tgcggctgtc ct
//
LOCUS       DASSGX010000051         5594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_117800, whole genome shotgun sequence.
ACCESSION   DASSGX010000051 DASSGX010000000
VERSION     DASSGX010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5594
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117800"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VFI12_00780"
     CDS             complement(<1..317)
                     /locus_tag="VFI12_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7054959.1"
                     /translation="MAHLIGRNLVSRRAIIGGALVAGAFGVPGSTGRRAARAARATSP
                     ETWRTWLLDSPDELRPPDPGDPAPAERDELIALQSRRSVETGVTVSRWGDGPAVLPWS
                     GIAL"
     gene            complement(340..936)
                     /locus_tag="VFI12_00785"
     CDS             complement(340..936)
                     /locus_tag="VFI12_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7054960.1"
                     /translation="MSCRVSGAGELYAASGAGVNLKMMLDGDGQPTVPLREGFGFDSY
                     YAQCVIEDNADRFAMDTFAMGRVVVEPHTFFMAMYANDISLVSVQDMGNGAKTAKLTG
                     DLGCLTEVGTATGKVGSRESKEPAFFEIEAIDGGHGGGTVGDKFSFTVYFDPKQAPVN
                     HGIFGPKPTFTGELIAGEVTIEPLVGKPLLAPSGTPSS"
     gene            complement(1023..1658)
                     /locus_tag="VFI12_00790"
     CDS             complement(1023..1658)
                     /locus_tag="VFI12_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7054961.1"
                     /translation="MFNRRHVDELQQVAGISRRAALVRGGATGALATVFATLGRGRES
                     LAQNATPMVAPAGWKAMHVEVSHVPHDPVSITVAGSGPPQRGDYFYIDAPIYAKGDEA
                     GSQIGTYQCFGAWTAAADDTSAENQRLTTVHYILQDGAIMGLINEAGADPNSHIGAVQ
                     GGSGAYAGAIGNFRQLSRTGTPIGVATPGGTPAAAPNIVDAVFDLLLPGQG"
     gene            2074..2568
                     /locus_tag="VFI12_00795"
     CDS             2074..2568
                     /locus_tag="VFI12_00795"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7054962.1"
                     /translation="METTSQQPISYMIRPMSDDAAHAIAGWKYPPPYNFYDAIDDPED
                     LDELLNPDRRRGRYFEAVDAAGVLVGFFEYKREVKPLEIGLGMRPDLTGRGLGLDFVR
                     AGMDFARTEFGAKSLSLAVAIFNERARTVYERAGFRPAGRYLHRIMGTDHEFLRMICE
                     PVSS"
     gene            2801..3565
                     /locus_tag="VFI12_00800"
     CDS             2801..3565
                     /locus_tag="VFI12_00800"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7054963.1"
                     /translation="MAFPDLTAFRPERLLLAPMLRSDEAELMDAPAQDEAELAANLRD
                     IRRVNRLLGGTAIVLHHLPAIVSAISPARPVTLLDLATGSGDIPLAISRWAKRRGLAL
                     TITASDYSDAILAAARQQLAAHPKIMLARYDARAVPLPDRSVDVVLCSLSLHHFPPDD
                     AVHVLREMNRLARHGFILNDLRRGRLGYAAAWIAAHATTRNRLTRNDAPLSIRRAYTP
                     AELGDLLHRAGIDDAEVTTHPWFRMAAVKITANGDG"
     gene            3558..4769
                     /locus_tag="VFI12_00805"
     CDS             3558..4769
                     /locus_tag="VFI12_00805"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET7054964.1"
                     /translation="MVERDAEAIVVGGGPAGSTIAADLASAGHRVLLLDKATFPRHKP
                     CSDYLNAGGAQILAEMGVLDEVMRAGAHRIEGMTIHAPGGNQFTANFAKAEPGRAAIG
                     LSRRCLDQLLLERARAAGATVVERAHVRDLVHEKGYVAGVEATIDGTTERIRAPLIIG
                     ADGRHSVVARALKLTAPLRWPRKTGLATHYRGIAGLDRFGEMHIVGHSLYAGLAPIEH
                     GMANLTIVLPSAAVEQRSGSIDELFADTLDRMPALAAQLDGAERVGGIRGVGSMGQRA
                     RRTTGDGFLLVGDAASFLDPFPGEGIYEALRAARMAAPVVSTALKTGDTSARALEPYR
                     IARRRAFTAKRQVCWIVQGFVNAPPLMNYVTDRLVEREELGLTLSGVLGNFRPATQAL
                     SPLFLARLLRP"
     gene            4786..5268
                     /locus_tag="VFI12_00810"
     CDS             4786..5268
                     /locus_tag="VFI12_00810"
                     /inference="COORDINATES: protein motif:HMM:NF015331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET7054965.1"
                     /translation="MQTANTVAMAGDLDRIVALAADIERWPVILPHYRWVTLLDGGGD
                     RKTVEMAARRGRFPVKWRAIQEIEHNGGPPIIWFTHIGGVTKGMEVAWTFEQNSDHVA
                     VTIDHEFAPPWPVVGNAVAERIIGPHFVEAIAGQTLGTIKAIVEDRDHRFDPDGRSRR
                     "
     gene            5265..>5594
                     /locus_tag="VFI12_00815"
     CDS             5265..>5594
                     /locus_tag="VFI12_00815"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HET7054966.1"
                     /translation="MKQRVAITGIGAITPIGSGVDELWAGVRAGRSSIRAIGRFNPDY
                     SASKVAGEVDFDPLQHMAPKRARRLDRFSQFALVSSQMALADAGMSAHEAACAGAGIY
                     IGSALGGV"
BASE COUNT          907 a         1994 c         1768 g          925 t
ORIGIN      
        1 agcgcgatcc cgctccaggg caacacggcg ggcccatccc cccacctcga aaccgtaacg
       61 ccggtctcga ccgagcgcct ggattgcagc gcgatcagct catcccgctc ggccggagcc
      121 ggatcgcccg gatccggcgg tcgcagctca tcgggcgaat cgagcagcca cgtccgccac
      181 gtctcgggag acgtcgcgcg tgccgctcgc gccgctcgcc ggccagtgct gcctggcacg
      241 ccgaacgcac cggcaacgag cgcgccgccg atgatcgcgc gccggctgac caggtttcgg
      301 ccaatcagat gggccattgc cgttctccgg gaccgccggt caggacgacg gggtgcccga
      361 gggcgcgagc aagggcttgc cgaccagggg ctcgatcgtc acctcgccgg cgatcagctc
      421 cccggtgaag gtcggcttcg ggccgaagat cccgtggttg accggcgcct gcttcgggtc
      481 gaagtagacg gtgaaggaga atttgtcgcc taccgttcct ccgccatgcc cgccatcgat
      541 tgcctcaatc tcgaagaagg ccggctcctt gctctcccgc gagccgacct tcccggtggc
      601 ggtgccgacc tccgtcaggc agccgagatc gccggtcagc ttggccgtct tcgccccgtt
      661 gcccatgtcc tgcacgctga ccaggctgat gtcgttcgcg tacatcgcca tgaaaaaggt
      721 gtgcggctcg accaccaccc gccccatcgc gaacgtatcc atcgcgaacc ggtccgcgtt
      781 gtcctcgatc acgcactggg cgtaatacga gtcgaatccg aacccctccc ggagcgggac
      841 agtcggctgc ccgtcaccgt cgagcatcat cttcaggttc acgccggcgc cggacgcggc
      901 gtagagctcg ccagcgccgg acacccggca agacatcgca atgctactga ttaatgccga
      961 tcccccggca atcaggcggc gacgatgaac tggctggatc aatgccctgt tcatggccgc
     1021 ccctagccct gcccgggcag gagcagatcg aagacagcat ccacgatgtt cggcgcagcc
     1081 gccggagtcc cacccggggt ggccaccccg ataggagtgc cggttcggct gagctgcctg
     1141 aagttcccga tcgcgccggc gtaggcgccg gaaccgcctt gcacggcccc gatatggcta
     1201 ttggggtcgg ctccagcttc attgatcagg cccatgatcg cgccatcctg gaggatgtaa
     1261 tggacggtcg tcagccgctg gttctcggcg gatgtgtcgt ccgcagcggc agtccaggcg
     1321 ccgaagcact ggtaggtgcc gatttgcgat cccgcctcat cgcctttcgc gtagatgggc
     1381 gcatcgatgt agaagtagtc tccgcgctgg ggcggcccac tgccggcaac cgtaatgctg
     1441 accgggtcgt gcgggacgtg gctgacctcc acgtgcatgg ctttccaccc ggccggcgcg
     1501 accatcggcg tcgcgttttg ggcgagggat tcccggcccc ggccaagcgt cgcgaaaact
     1561 gtggccaagg cgccggtcgc accaccccgc acgagcgcgg cccgtcggga aattccggcc
     1621 acctgctgca attcatcgac gtgccttcgg ttgaacatgg tgattcctcc gcttctctta
     1681 catcgacgcc ggtactacgc ggaatggagc ggtccatgat tgccggatca gccaccgggt
     1741 tgcatcccca gaaacgcgcc gatcgtgatc aggcaagtga tcccacgatg cgaaaacgcc
     1801 gcctcgcatc tatgcgcgac gcaagcggct gacctgattt cagattccgg gccgggtgaa
     1861 gatcggattg aagggcgctc atggcgaccc tccctcgtag cacgatctgt ttcgcggtca
     1921 cgttggattg gagttgttct tcgtctcgta aagtatcaga cagtgcccgc gtgtcaatcc
     1981 tgtccggaca cggaaacgcc ccgatcagac gcgttttgct agcgtgggct acggtatgtc
     2041 ggagaagcgg ggcgcgggca ggcgaggagc gcgatggaga cgacgagcca gcagccgatc
     2101 agttacatga tccggccgat gagcgacgat gccgcgcacg ccatcgccgg ttggaaatac
     2161 ccgccgccct acaacttcta cgacgcgatc gacgatcccg aggacctgga tgagctgctc
     2221 aacccggacc gccgccgggg tcgttacttc gaagcggtcg atgccgccgg cgtcctggtc
     2281 ggcttctttg agtacaagcg cgaggtcaag ccgctcgaga tcgggctcgg gatgcggccg
     2341 gatctgacgg ggcgcggcct cgggctcgac ttcgtccggg ccggcatgga cttcgcccgc
     2401 accgaattcg gcgcgaagtc actgtcattg gccgtcgcca tcttcaacga gcgcgcccgc
     2461 acggtctacg agcgcgccgg cttccggccc gccggacgct acctccaccg catcatgggc
     2521 accgaccacg aattcctgcg catgatctgt gagcccgtat cgagttgacg gatgaccgat
     2581 tgtgtaggga tttgcggggg ctaatccgcg ctgccggtgg acccgggcac gagcattccc
     2641 atgcaaccgg gcgttcggga gcgcgcgggc agatgccatc tgcccctacc ctcgttcctt
     2701 ccaacctcca tccgctacca acccttcgct gtcgcctgtg taacgattcg agccgtaccg
     2761 gaacggtacg ctgattgcca tccgaaggag catgcaccgg atggcctttc ccgacctgac
     2821 cgcgttccga cccgagcgac ttctgctcgc cccgatgctt cggagcgacg aggctgagct
     2881 gatggatgcc cctgcccagg acgaggccga gctggccgcg aacctgcgcg acatccgccg
     2941 cgtcaatcgc ctcctcggcg gcaccgcgat cgtcctgcac cacctgcctg caatcgtgag
     3001 cgccatttcg cccgcccggc cggttaccct gctcgacctt gcgaccggct ccggcgacat
     3061 cccgctcgcc atctcccgct gggccaagcg gcgcggcctc gccctgacca tcaccgcgag
     3121 cgactactcg gacgcaatcc tggcggccgc ccggcagcag ctcgccgccc acccaaagat
     3181 catgctcgcc cgctacgatg cccgtgccgt gccgctgccc gacaggtcgg tcgatgtcgt
     3241 cctctgctcg ctctcgctgc accacttccc acccgacgat gccgtgcacg tcctgcgcga
     3301 gatgaatcgg ctcgcccggc acggcttcat cctgaacgac ctccgccggg gccggctcgg
     3361 ctacgcggcc gcctggatcg ccgcccatgc caccacccgc aaccgcctga cccgcaacga
     3421 tgccccgctc tcgattcgcc gcgcctacac cccggccgaa ctgggtgatc tgctccaccg
     3481 cgccgggatc gacgacgccg aggtcactac ccatccctgg ttccggatgg cggcggtcaa
     3541 gatcacggcc aacggcgatg gttgagcggg acgccgaggc aatcgtggtg ggcggcggtc
     3601 cggccggcag cacaatcgcg gccgacctcg catcggccgg tcatcgcgtg ctgttgctcg
     3661 acaaggcgac gttcccgcgc cacaagccct gctccgatta cctgaatgcc ggcggggcgc
     3721 agatcctggc cgagatgggc gtgctcgacg aggtcatgcg agcgggagcg caccgtatcg
     3781 aggggatgac gatccatgcg ccaggcggca atcaattcac cgccaatttc gcgaaggccg
     3841 aacccggccg agccgcaatc ggcctttctc gtcgctgcct cgatcagctc ctgctcgagc
     3901 gggccagggc cgccggcgcg accgtggtgg agcgcgcgca cgttcgggac ctcgtccacg
     3961 aaaagggata tgtcgccggc gtcgaggcca cgatcgacgg cacgaccgag cgcatccgtg
     4021 ccccgctcat catcggcgcc gatggccggc actcggtcgt cgcccgcgcg ttaaagctga
     4081 ccgcgcccct ccgctggccc cgcaagaccg gcctcgccac ccattaccgg ggcatcgccg
     4141 gtctcgatcg cttcggcgag atgcacatcg tcggccactc gctctacgcc ggacttgccc
     4201 cgatcgagca cgggatggcc aacctgacga tcgtcctccc cagcgcggcg gtcgaacaac
     4261 gctccggctc gatcgatgag ctttttgccg ataccctcga ccgcatgccg gccctcgctg
     4321 cgcagctgga cggagccgag cgagtcggcg gcatccgggg cgttgggtcg atgggtcagc
     4381 gcgcccgtcg caccactggc gacgggttcc ttttggtcgg cgacgcagcc tcattcctgg
     4441 atccgttccc cggtgaaggc atctacgagg cactccgcgc cgcccggatg gccgcgccgg
     4501 tcgtcagcac cgccctcaag accggcgaca cttcggcccg tgcccttgag ccgtaccgga
     4561 tcgcccgccg ccgcgccttc acggccaagc ggcaggtctg ctggatcgtc cagggcttcg
     4621 tcaatgcgcc gccgctgatg aattacgtaa ccgatcgact cgtcgagcgc gaagaactgg
     4681 gtctgaccct cagcggcgtg ctcggcaatt tccggccagc cacgcaggcc ctctcgcccc
     4741 tcttcctcgc ccggctactg cggccatagc aacgacagga gcgccatgca aaccgccaac
     4801 accgtagcaa tggccggcga cctcgaccgc atcgtcgcgc tcgcggcgga catcgagcgc
     4861 tggccggtca tcctccccca ctaccgttgg gtcacgctgc tcgacggcgg cggggatcgg
     4921 aagacggtcg agatggctgc ccggcggggt cggttcccgg tcaaatggcg cgcaatccag
     4981 gagattgagc acaacggtgg cccgcccatc atctggttca cccacatcgg cggcgtgacg
     5041 aaggggatgg aggtcgcctg gaccttcgag cagaactccg atcacgtggc cgtcacgatc
     5101 gaccatgagt tcgcgccgcc ctggccggtc gtcggcaatg ccgtcgccga acggatcatc
     5161 ggtccgcatt tcgtcgaggc gatcgccggc cagacgctcg gcacgatcaa ggcgatcgtc
     5221 gaggaccggg atcaccgctt cgacccggat gggcggtcac gacgatgaaa cagcgggtcg
     5281 ccatcaccgg catcggtgcg atcaccccga tcggcagcgg cgtcgacgag ctctgggcgg
     5341 gtgtccgagc cggaagatcg tcgatccggg cgatcggccg gttcaatccc gactattcgg
     5401 cgagcaaggt ggccggcgag gtcgatttcg atccgctcca gcacatggcg ccgaagcgcg
     5461 cccgccgcct ggatcgcttc tcgcaattcg cgctggtctc gagccagatg gcactagccg
     5521 atgccggcat gtcggctcac gaggcggcct gcgccggggc aggcatctac atcggctcgg
     5581 cgctcggcgg cgtg
//
LOCUS       DASSGX010000052         4759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_553049, whole genome shotgun sequence.
ACCESSION   DASSGX010000052 DASSGX010000000
VERSION     DASSGX010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553049"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="VFI12_00820"
     CDS             <1..865
                     /locus_tag="VFI12_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7054967.1"
                     /translation="GPGPAQLFCEVVGFGAMLLTRYQVYPWLIGLTAAAIVVGLLVLA
                     IVGRTTLAAATDIYGEYPHLFIPLGLLIIPIGIAATGLQYLVIEYPPGKQIFEVMNKS
                     PGARLAAALTVGGFQQLAGLIVIGPTVIVLVSDIQAGRTPSLRRAYEVVLAGIGRLAA
                     AVLGPVVIVGLLALSVVGIPWAVTRAVRWFFVAQAAIVDRATPKEARARSARAVSGHW
                     WRTVSIVFFLTVLGNVAGPLIGIYLLVFDARSVEFVNLVSSLVYAALLPLSVVGYTQL
                     YQQLERGHWRG"
     gene            complement(902..1744)
                     /gene="prmC"
                     /locus_tag="VFI12_00825"
     CDS             complement(902..1744)
                     /gene="prmC"
                     /locus_tag="VFI12_00825"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HET7054968.1"
                     /translation="MATINEAIRDVQRQFAESGIDTARLDAELLVGHALGMSRTQLLA
                     NLRDPISDDLAPAVHILAARRLAGEPVAYILGRKEFLGHDFIVTPAVLSPRPETELLV
                     EWAVRWLLNRPEATVVDIGTGSGAIAIGVALNTPPTVSITAIDVSDDALGVARANADR
                     LCPGRIAFQTGNLLSGVQRRFDLVLANLPYLRPDQITGNRDLSAEPRLALDGGRLGLD
                     LIERLVEELPRHLAVGGGVALEIDPSQGHVVAQRLAAALPGATVTIENDLAGLDRFVT
                     AVRG"
     gene            complement(1757..2857)
                     /gene="prfA"
                     /locus_tag="VFI12_00830"
     CDS             complement(1757..2857)
                     /gene="prfA"
                     /locus_tag="VFI12_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003202249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HET7054969.1"
                     /translation="MSAFLSTLAELKRRYDELDHQMADPAVLSDPGKLADLGRERAEL
                     EEVVSVYNELQDSERELKDAEELADDSDPELSGLADDEIRRLKALREQYVSRLRSLLV
                     PKDPNDEKNVIVEIRAGTGGEEAALFAADLYRMYVRYAEKHRWKIDVLSSTETDNGGF
                     REIIFEVSGKGAYSHLRYESGVHRVQRVPATESQGRIHTSTASVAVLPEAEDVDIKVN
                     DNELRIDVYRSTGHGGQSVNTTDSAVRITHLPTGLVVTCQDEKSQLKNKLKAMTVLRS
                     RLYEIEQEKLSAERGDARRSQIGSGERSEKIRTYNFPQDRITDHRIKVNASNLPSIMD
                     GNIDVFVNELRAADEAERLRDAGLEDSPLAAD"
     gene            complement(2917..3591)
                     /gene="thpR"
                     /locus_tag="VFI12_00835"
     CDS             complement(2917..3591)
                     /gene="thpR"
                     /locus_tag="VFI12_00835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HET7054970.1"
                     /translation="MPRDRRKFTKERPLDPDSVEVQWRLFVATDFPESHRVLLDEFLA
                     IVSEADVPIRWVGANAAHMTLHFIGEVPIETAELLRLGFSSAAGSTHPFSLHVDGAGA
                     FPNLDKPQIVWLGLAGDVASLRRLHRASETFLLGFDIVTEQREFKPHITLGRARQALQ
                     SPEINALVRLMRSDEVQQKLAALAEPFRVDHLTLYRSHLSHEGASYEPLATARLSGRG
                     KALGRL"
     gene            3642..4505
                     /gene="pheA"
                     /locus_tag="VFI12_00840"
     CDS             3642..4505
                     /gene="pheA"
                     /locus_tag="VFI12_00840"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HET7054971.1"
                     /translation="MVTAFLGPRGTFSEDAALSYAGPDGELLPMTSIPALVAAVETGL
                     ADNAVLPIENSIEGAISTTLDLLIHETELSIVDELVVRVRLYLITVPGASLSDITVIS
                     SHANPLGQSRRFLERCLPKATQIASISTAAAVREMMEAGDPSRAAIGTLSAATMFGGQ
                     ILARDIQDAQTNVTRFVVLADRKTKPTGDDKTSIAFLLKANVPGALFRVLRPFAEAGI
                     QLTKLESRPSRSELLEYVFLCDFFGHRDDPVIARALDAMAEECSMLKVFGSYPRFHLE
                     SMTFKSGDDGQ"
     gene            4502..>4759
                     /locus_tag="VFI12_00845"
     CDS             4502..>4759
                     /locus_tag="VFI12_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7054972.1"
                     /translation="MTSPLAIADSILTAYLRAGLDQLPGVRLTDGRDGFVVVETARIG
                     SLPEHTIHPDVGIVVRDRGVVTLQSAVRPDELDRPAVWLRVS"
BASE COUNT          757 a         1513 c         1600 g          889 t
ORIGIN      
        1 cggccccgga ccggcccaac tcttctgcga ggtggtcggg ttcggcgcga tgctgttgac
       61 gcgctatcag gtctatccgt ggctgatcgg gctcactgcc gccgccatcg ttgtcgggct
      121 gcttgtcctg gcgattgtgg ggcgcacgac cctcgccgcc gccacggaca tctacggcga
      181 atacccgcac ctgttcattc cgctgggcct gcttatcatc ccgatcggca tcgcggccac
      241 tgggttgcag tacctggtga tcgagtatcc acccggcaag cagatcttcg aggtcatgaa
      301 caagagcccg ggcgcccggt tggcagccgc tttgaccgtc ggcggattcc agcagcttgc
      361 gggcctgatc gtcattggcc caaccgtgat cgtgctggtg tccgatatcc aggccgggcg
      421 cacgcccagc ctcaggcgcg cgtacgaggt tgtcctcgcc ggaattggcc ggctcgcggc
      481 tgctgtcctg ggcccagtcg tgattgtcgg cctgctggcg ctctccgtgg ttggcatccc
      541 gtgggcagtc acgcgcgcgg tccgctggtt cttcgtggcg caggccgcga tcgtcgaccg
      601 ggcaacgccg aaagaagcaa gggccaggag cgcccgggcc gtttccgggc actggtggcg
      661 cactgtcagt atcgttttct tcctgacggt gctcggcaat gttgccgggc ccctgatcgg
      721 gatttacttg ttggtgttcg atgcgcgatc ggtcgagttc gtcaacctgg tcagcagcct
      781 ggtctacgcg gcgctgcttc cgctgtcagt cgttggctac acccagctct accagcagct
      841 ggagcgcggc cattggaggg ggtgacgcgc gagacgcata ccaggagcgc cccgctccgc
      901 atcacccccg aaccgccgtc acgaaccggt caagacccgc cagatcgttc tcaatcgtga
      961 ctgtcgcacc gggaagggca gcggccagtc tctgcgcgac aacatgtccc tgggatggat
     1021 cgatctccag tgcgaccccg ccgcccacag cgaggtgcct gggcagctcc tcgacgaggc
     1081 gctcgatcag atcgagcccc agtcgcccgc catcgagcgc cagccgcggc tcagccgaca
     1141 ggtcccgatt gccagtgatc tggtcgggcc ggaggtacgg gaggttggcg aggaccaagt
     1201 cgaaccgtct ctgaacgccg gacaggagat tgccggtctg aaatgcgatg cggcccgggc
     1261 agagccgatc ggcattcgct cgcgccacgc ccaatgcatc gtccgagacg tcgatcgcgg
     1321 tgatcgagac ggtcggcggc gtgttcaggg cgacgccgat cgcgatcgcg ccactgccgg
     1381 ttccgatatc gacgacggtc gcctccgggc gattcaagag ccaccggacg gcccactcga
     1441 cgagcaactc ggtctcgggt cgcggcgaca ggaccgccgg ggtgacgatg aagtcgtgcc
     1501 caaggaactc cttgcggccg aggatgtagg ccaccggctc cccggcgagt cgacgggcgg
     1561 ccaggatatg aaccgccggc gccaggtcgt ccgaaatggg gtcgcggagg ttcgcgagga
     1621 gctgtgtccg gctcataccg agcgcgtggc cgacaagcag ctccgcgtcg aggcgtgcgg
     1681 tgtcgattcc cgactcggca aattgccgct gcacgtcgcg aattgcctcg ttgatagtcg
     1741 ccaaatcagg agtcgattag tcagcggcga gcgggctgtc ttcgaggccg gcgtcgcgta
     1801 gccgctcagc ctcgtccgcc gcccggagct cgttcacgaa gacgtcgatg ttgccgtcca
     1861 tgatcgaggg caggttgctc gcgttgacct tgatccggtg gtcggtgatc cggtcttgcg
     1921 ggaagttgta ggtgcggatc ttttcgctgc gctcgccgct gccaatctgc gagcggcggg
     1981 catcgccgcg ctcggcggac agcttctcct gctcgatttc gtagaggcgg ctacggagga
     2041 cggtcatcgc cttgagcttg ttcttgagct ggctcttctc gtcctggcag gtcacgacca
     2101 gcccggtcgg caggtgcgtg atgcggacgg ctgagtcggt cgtattgacg ctctggccgc
     2161 cgtgaccggt cgagcggtag acgtcgatcc ggagctcatt gtcgttgacc ttgatgtcga
     2221 catcctcggc ctcgggcagc accgccacgc tggcggtcga ggtgtggatc cggccctggc
     2281 tttcggtggc cgggactcgc tgcacacgat ggacgccgct ctcgtagcgg aggtggctat
     2341 acgcgccctt gccggagacc tcgaagatga tctcccggaa cccgccgttg tcggtctcag
     2401 tgctggagag gacgtcgatc ttccagcggt gcttctcggc gtagcggaca tacatccggt
     2461 agaggtcggc cgcaaagagg gccgcttctt cgccgccggt gccggcccgg atctcgacga
     2521 tcacgttctt ctcgtcgttc gggtccttgg gcacgagcag ggatcgcagg cgcgagacgt
     2581 actgctcgcg cagcgctttc agccgccgga tctcgtcatc cgcaagaccg gagagctccg
     2641 gatcggagtc gtcggccagc tcttcggcat ccttgagctc ccgctcgctg tcctgcagct
     2701 cgttgtagac ggagacgact tcctcgagct cggcccgttc ccgtccgagg tcggcaagtt
     2761 tgccggggtc gctgaggacg gccggatcgg ccatttggtg gtcaagttcg tcgtagcggc
     2821 gcttcagctc cgccagggtc gagagaaatg cgctcatggc tcagtgattt cttggtgata
     2881 gggcgtacga cctggcgcac gcgcagatca cgctaattat agtcgaccga gtgccttgcc
     2941 tcgtccggaa aggcgggcag tggccagtgg ttcgtaagac gcaccctcgt gcgagaggtg
     3001 gctgcggtag agggtaaggt ggtcgacacg gaacggttcg gcaagcgcgg ccagcttctg
     3061 ttgtacctca tcggaccgca tcagccgtac aagcgcgttg atttccggcg attgcaatgc
     3121 ctggcgggcg cggccgagcg tgatgtgcgg cttgaattcg cgctgctcgg tcacgatgtc
     3181 gaagccgagc aggaaggtct cgctagccct gtgcaaccgg cggagcgacg cgacatcacc
     3241 ggccaggccg agccagacga tctgcggctt gtcgaggttc gggaaggccc cggcgccatc
     3301 cacgtgcagc gagaagggat gggtgctgcc ggccgccgag ctgaagccca gccggagcaa
     3361 ttcggcggtc tcgatcggca cctcaccgat gaagtgcaac gtcatgtgcg cggcgttcgc
     3421 accgacccag cgaatcggca catcggcctc gctaacgatc gcaaggaatt cgtcgagcag
     3481 gacgcgatgg ctttccggga agtcggtggc gacgaacagt cgccattgca cttcgaccga
     3541 atccgggtcg agtggtcgct ctttcgtgaa tttgcgtcgg tctcgcggca tgataggcag
     3601 tatacgtagg agagacacgc gaacgagatg gtgacgatac gatggtgacc gcattcctgg
     3661 gtccgcgcgg gactttcagc gaggatgccg cgctcagcta tgcggggcct gatggcgagc
     3721 tgctgccaat gaccagcatc ccggcgctgg tcgccgcggt cgagacgggc ctggcggaca
     3781 acgccgtgct gccgatcgag aattcgatcg agggggcgat cagcaccacc ctcgatctgc
     3841 tgatccacga aaccgagctg agcatcgtcg acgagctggt ggtgcgcgtc cgcctctacc
     3901 tgatcaccgt gccgggcgcg tcgctgagcg atatcaccgt gatctcgtcc cacgccaacc
     3961 ccctgggcca gagtcgccgc ttcctggagc gctgcctgcc gaaggccacc cagatcgcct
     4021 ccatcagcac ggctgcggcg gtgcgggaga tgatggaagc gggcgacccg tcgcgggccg
     4081 cgatcgggac attgagcgcg gcaaccatgt tcggcggaca gatcctcgcc cgggacatcc
     4141 aggacgccca gacgaacgtg acccgcttcg tcgtccttgc cgatcgcaag acgaagccaa
     4201 ccggcgacga caaaacatcg atcgcgttcc tgctcaaggc caacgtgccg ggcgcgttgt
     4261 tccgcgtcct gcggccgttc gctgaggcgg gcatccagtt gaccaagctg gagagccggc
     4321 cgtcgcgcag cgagctactc gaatacgtct tcctctgcga ctttttcggg caccgggacg
     4381 atcctgtcat cgcgcgcgcg ctcgatgcca tggccgagga gtgctcgatg ctcaaggtgt
     4441 tcggaagcta tccccgcttc cacctcgagt cgatgacctt caagtcaggg gacgacgggc
     4501 agtgacctct cccctcgcca tcgccgattc catcctgacc gcgtatctcc gggctggttt
     4561 agaccaattg ccgggggtgc gattgaccga cggccgcgat gggttcgtag tagtcgagac
     4621 ggcccgaatc ggctcgctgc cggaacacac cattcatccc gacgtcggca tcgtcgtgcg
     4681 ggatcgcggc gtggtgaccc tgcagtcggc ggtccggccg gatgagttgg atcggccggc
     4741 cgtctggctg cgcgtgtcg
//
LOCUS       DASSGX010000053         2465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1534144, whole genome shotgun sequence.
ACCESSION   DASSGX010000053 DASSGX010000000
VERSION     DASSGX010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1534144"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="VFI12_00850"
     CDS             <1..786
                     /locus_tag="VFI12_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET7054973.1"
                     /translation="AAYERTRAMLVDEGFAPAETAMLVEAFIPGVEVAVEGLMTDGEL
                     TVLALFDKPDPLDGPYFEETIYVTPSRLPDGTQRAIAETTSRMAAALGIRHGPVHAEL
                     RINEEDIWPIEVAGRSIGGMCSTILEFGAGMPLEEIILRHAVGGDLPSTRRSGQAVGV
                     MMIPIPGEGVLREVGGIAEACQVPGITGIEITAPLNQPIQPLPEGASYLGFIFATCTE
                     PAEVESALKQAHARLDIRIAPMLKLRVEGGELREQKSGVRPKT"
     gene            complement(797..1108)
                     /locus_tag="VFI12_00855"
     CDS             complement(797..1108)
                     /locus_tag="VFI12_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HET7054974.1"
                     /translation="MTETTPRMVNCVKLGRPAPGLERPPFPGELGQRIYENISAEAYD
                     LWKPQMTILINHYGLNPADPEARVFLRQQMEDFFFGPGAQMPEGWVAPDADVPARPRK
                     K"
     gene            1179..2069
                     /locus_tag="VFI12_00860"
     CDS             1179..2069
                     /locus_tag="VFI12_00860"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HET7054975.1"
                     /translation="MSNPNATTRWRCLFFGMPCAFSSPVLAALVRPDVELVGVVTPQT
                     APDPEPYRLIRGLRSRATIMLSARAGHFEAPRYLVRDIANPRLHAELAALAPDLIIVA
                     CYPRLIPQPIRDAARIAALNIHPSLLPRHRGPDPLFWTFHAGDNISGITIHHLSGRFD
                     AGDILLQDTTSIHADESLSDLEQRLARLAANQVGRLISGLPSLPLPRPQNPALATSEP
                     LPTESDRTIDATWSITRARRFIAGVAGSHGPLFWQDDGKSKAVSGLAEPRSGQELALA
                     DGVLNVALRSFGGADRASHF"
BASE COUNT          423 a          858 c          756 g          428 t
ORIGIN      
        1 gctgcctacg agcgcacccg agcgatgctg gtcgacgaag ggttcgcccc ggccgagacg
       61 gcgatgctgg ttgaagcgtt catcccgggc gtcgaagtgg ccgtcgaggg gttgatgacc
      121 gacggcgaat tgaccgtact cgccctcttc gacaagccag acccgttgga cggcccctat
      181 ttcgaggaga caatctacgt taccccctcg cgcttgccgg acgggacgca gcgagccatc
      241 gccgagacga ccagccggat ggccgccgcg ctcggcatcc gccacggacc ggtccacgcc
      301 gaattgcgaa tcaatgaaga ggatatctgg ccgatcgagg tcgccggccg gtcgatcggc
      361 gggatgtgct cgaccatcct cgaattcggg gcggggatgc cgctcgagga gatcatcttg
      421 cgccacgccg tcggcggtga cctcccgtcc acccggcgca gcggacaagc ggtcggcgtg
      481 atgatgatcc cgatccccgg cgaaggggtg ctgcgggagg tcggcgggat tgcggaagcg
      541 tgccaggtgc ccggcatcac cgggatcgag atcaccgcgc cgctcaacca gccgatccag
      601 cccctccccg aaggcgccag ctacctcggc ttcatcttcg ccacgtgcac ggagcccgcc
      661 gaagtcgaat ccgccctgaa gcaggcccac gcccggctcg acatccgcat cgcgccgatg
      721 ctcaagttga gggtggaggg tggagagttg agggaacaga agtcaggagt caggcccaaa
      781 acctgaaacc caagacctac ttcttccggg gccgagccgg gacatcggcg tccggcgcga
      841 cccagccttc gggcatctgg gcgcccggac cgaagaagaa atcctccatc tgctgacgca
      901 ggaagacgcg ggcctcggga tcggccgggt ttaggccgta gtggttgatc aggatcgtca
      961 tctgcggctt ccagagatcg tacgcttcgg cggatatatt ctcgtagatg cgttggccga
     1021 gctcgcccgg aaacggcggt cgctccagcc cgggcgccgg gcgtccgagc ttgacgcaat
     1081 tcaccatccg cggggtagtc tcagtcattg atccgacctc agtttgtggg cgcgtcgatc
     1141 cgtcctgatc gccgtatcgt ccggcacgga gccgccgcat gtcaaacccg aacgcaacaa
     1201 cccggtggcg atgcctcttc ttcgggatgc cgtgcgcctt ctcatcgcca gtcctggcgg
     1261 ccctcgtccg gcctgacgtc gagctggtcg gagtcgtcac gccgcagacg gcgcccgacc
     1321 ccgaaccgta cagattaatt cgcggcctgc gctcccgggc gacaattatg ctttccgcgc
     1381 gcgctgggca tttcgaggcg ccccgctacc tcgtccggga catcgccaat ccgcgcctcc
     1441 acgccgaact ggccgcgctc gcgcccgacc tgatcatcgt cgcctgctac ccgcgactga
     1501 ttccgcagcc gatcagggac gcagcccgga tcgcggcgct caacatccac ccgtcgctcc
     1561 tgccccgcca tcgcggaccg gatccgctct tctggacctt ccacgccggc gacaatatct
     1621 ccggcatcac gattcaccac ctctccggcc ggttcgatgc cggcgacatt ctcctccagg
     1681 acacgaccag tatccatgcc gacgagtcgc tctccgacct ggaacagcgc ctggctcgcc
     1741 tcgccgcaaa tcaggtcggc cgcttgatct ccggcctgcc gtccctcccg ttgccccggc
     1801 cgcagaatcc ggcgcttgcc acctccgagc cgttgcccac tgaatcggat cgtacgatcg
     1861 atgcgacgtg gtccataacg cgagcgcgtc ggttcatcgc cggtgtcgcg ggcagccacg
     1921 gaccgctctt ctggcaagac gatggcaaat ccaaagccgt ctccgggctc gcggagccac
     1981 ggtcaggaca ggaactcgcg ctcgccgatg gcgtcctgaa tgtagccttg cgatcgtttg
     2041 ggggtgctga tcgggctagc catttttgac aggccgccaa agctgtgaga taacccgggg
     2101 caatcgctca aagaaatggc atctaaaggc gctttacgct gatcgctggc tacgatcaat
     2161 gccgggtccc aaagcgagca cgtaccccga tccgtcgagg gatggaccgg gcgacccgca
     2221 ccgcatgcgc gtgctggctg gtcgaatcga gaacgcgcga ttcgctcact gcttgaatcg
     2281 ccaccttgcg tcagtcggat caggcccggt cgacccgcct ccgaccatgt cctgatcggg
     2341 gtgacgcgaa ggaacggccc atcatggctt ttctgcctgt cactcccggc acgggcaatt
     2401 tccgtgtccc caaaatgctc ctggccgccc tgatcgtggc cagcctgctc gtcccggtga
     2461 tctcc
//
LOCUS       DASSGX010000054         3263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_9740, whole genome shotgun sequence.
ACCESSION   DASSGX010000054 DASSGX010000000
VERSION     DASSGX010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9740"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..896
                     /locus_tag="VFI12_00865"
     CDS             <1..896
                     /locus_tag="VFI12_00865"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HET7054976.1"
                     /translation="TQDATPARIVQMMVGRALSDLFQKEASEAIGKPVLEVRNLSRAR
                     AKGDTSSTSIHDINFSIRAGEIVGLAGLVGSGRTELARSVFGADRIDSGEIFVDGTRA
                     TLKHPLDAIELGIGLVPEDRKQQALVLDLPVRENVSLANLDAITRAGFIRRSTERSIT
                     QRFIDALQIRTPGMEQKVVNLSGGNQQKVVLAKWLALNPKLLIVDEPTRGIDVGAKAE
                     VHLLLSRLAQQGVGILMISSELPEILGMSDRILVMREGRIAGELDRADATQEAIMSLA
                     TGLEHVRAAATDQVDSAVRPA"
     gene            917..1267
                     /locus_tag="VFI12_00870"
     CDS             917..1267
                     /locus_tag="VFI12_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7054977.1"
                     /translation="MAGSVTGNVIPAPGGMVSPQAPGMRPDRTTIPTHVDESIPPAAV
                     KVRPLTQYGTAEELMDVFRRKPMAQLEPSDFEQAPATAEEMRKANPADLMPIRPLPPL
                     GSGELIPEIGPGEF"
     gene            1304..2323
                     /locus_tag="VFI12_00875"
     CDS             1304..2323
                     /locus_tag="VFI12_00875"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7054978.1"
                     /translation="MSASSAAPASISEEQRGIDVPALLGKFAPVIFLLLVMAFFSFFE
                     PGFRTTNNFLNIIRAVSFFGILGVGMTFVILTAGIDLSVGSALAFCGIVCASAAKGSR
                     GLLTGKDEGGIYVLYALLAAIGVGLLIGLIHGIFIAYLNVPAFIVTLGGLGAWRGATL
                     LWSNGQPISNFSKDFTHWGKGSVGSVPIPVIVFVGMGLIAHIVLKYTRYGRWVYALGG
                     NAEAARLSGLNTKMIITSVYVISGFCAGVAGFLLTSRLNSAEQVAGQSYELQAIAAVV
                     IGGTSLFGGAGGVIGTLIGAVLIGVLNNGLVQMNVSAYYQPIVVGIIIVLSVYVDQVV
                     KSRRR"
     gene            2420..>3263
                     /locus_tag="VFI12_00880"
     CDS             2420..>3263
                     /locus_tag="VFI12_00880"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET7054979.1"
                     /translation="MSKSISEVTERRTTRRTVIKTAAIAGLAAPFMSMPSMAGAQTTY
                     KLAFSVPGLNFPFFVHMMDLAKKHVQEKYPDVEFIELDGQENGAPSSTKQSNDIDAII
                     AQKVNALVISPNDVAALAPAVQSAVDAGMIVVTVDRNVTDVQTLAHVGADNVEGGREQ
                     GRYLIEILPDGGDVIELQGQPGAAPAIDRHKGLDEIISAQDKVKIVVSQTAQFARAQA
                     VTVLEAALQTEEAKNVAAVVCANDDMAFGASEALSDAGKTVPVLGFDALPEALQAIQA
                     GTMAA"
BASE COUNT          579 a         1069 c         1049 g          566 t
ORIGIN      
        1 tgacccagga cgcgacaccc gcccggatcg tccagatgat ggtcggccgc gcgctctcgg
       61 acctgttcca gaaggaggcc agcgaggcga tcggcaagcc ggttctcgaa gtccgtaacc
      121 tgtcccgcgc ccgcgccaaa ggcgacacgt catcaacctc aatccacgac atcaacttct
      181 cgatccgcgc cggggagatc gtcgggctgg ccgggctggt cggctccggg cggaccgagc
      241 tggcgcgctc ggtcttcggc gccgaccgga tcgactccgg cgagattttc gtcgacggca
      301 cgcgcgccac actcaagcac ccgttggacg cgatcgagct cggcatcggc ctcgtaccgg
      361 aagaccggaa gcaacaggcg ctcgttcttg acctgccggt tcgcgagaac gtcagcctcg
      421 ccaatttgga cgccatcacg cgcgccggtt tcatccgccg ctcgaccgag cgctcgatca
      481 cgcagcgctt catcgatgcg ctccagatcc gcacgccggg gatggagcag aaggtggtca
      541 acctcagcgg cggtaatcag caaaaagtag tgctggccaa gtggctggcg ctcaacccga
      601 agctgctgat cgtcgacgag ccgacgcgcg gcatcgacgt cggcgccaag gcggaagtgc
      661 acttgctcct gagtcgcctc gcgcagcagg gcgtcggaat ccttatgatt tcatccgagt
      721 tgccggagat cctcggcatg agcgatcgca tcctggttat gcgggagggc cggatcgccg
      781 gggagctcga tcgggccgat gccacgcagg aagcgatcat gagcctcgcc accggcctcg
      841 aacacgtccg ggcagccgcg acagaccagg tagattccgc ggttcgcccc gcgtagcaga
      901 agggttggga caccagatgg caggatcagt aacaggcaac gtcattcccg cgccgggcgg
      961 gatggtttca ccgcaggcgc cgggcatgcg gcccgaccgg accacgattc cgacgcacgt
     1021 cgacgagtcg attccgccgg ccgcggtcaa ggtccggccg ctgacgcagt acggcacggc
     1081 tgaggagttg atggatgtct tccggcggaa gccgatggcc cagctcgagc cgtctgactt
     1141 cgagcaagcg ccggccaccg ccgaggagat gcggaaagcg aacccggccg acctgatgcc
     1201 gatccggccg ctgcccccac ttgggtccgg tgagctgatc ccggaaatcg gccccggcga
     1261 gttctaacgc ggtttcgatc aggtcacggg agagcaaaac ggcatgagcg catcgagcgc
     1321 ggcgccagca tcaatcagcg aggagcagcg cgggatcgat gtaccggccc tgctcggcaa
     1381 gttcgctccg gtcatcttcc tcctgctggt gatggccttc ttctcgttct tcgagcccgg
     1441 tttccggacg acgaacaact tcctgaacat catccgcgcc gtttccttct tcgggatcct
     1501 cggggtcggc atgaccttcg tcatcctgac ggccggcatc gacctctcgg tgggctcggc
     1561 cctggccttt tgcggcatcg tctgcgcctc ggcggccaaa ggctcccgcg gactgctgac
     1621 cggcaaggac gagggcggca tctatgtcct ctacgccttg ctggccgcga tcggcgtcgg
     1681 actcctgatc ggtctgatcc acggcatctt catcgcgtat ttgaacgtgc cggccttcat
     1741 cgtcacgctt ggcggccttg gtgcctggcg cggggcaacg ctgctctggt cgaacggcca
     1801 gccgatcagc aacttctcga aggatttcac ccactggggc aaaggctcgg tcggctcggt
     1861 gccgatcccg gtcattgtct ttgtcgggat gggcctgatc gcgcacatcg tgctcaaata
     1921 cacccggtat ggccgctggg tctacgcact cggcggaaat gccgaggccg cgcgcctctc
     1981 cggcctgaac acgaaaatga tcatcaccag cgtctacgtg attagcggtt tctgcgccgg
     2041 tgtggcgggc ttcctgctga cctcgcgcct caactcggcg gaacaggttg ccgggcaatc
     2101 ctacgagctg caggcgattg ccgcggtggt gatcggcggg accagcctct tcggcggggc
     2161 gggcggcgtc attggcaccc tgatcggcgc agtcctgatc ggcgtgctga acaacggcct
     2221 ggtccagatg aacgtctcag cctactacca gcccatcgtt gtcggtatca tcatcgtgct
     2281 cagcgtgtac gtggaccagg tagtaaaatc ccggcgtcgc taacgccgtg gacccgcatg
     2341 gtcctgccaa actcggccta acgtggctcg ctccgcatcg tgcgcgggcg aatcgtgact
     2401 cattgaggag gaaagcgctg tgtcgaagtc gatctcagag gtcaccgaac ggcggacaac
     2461 gcgccgaacc gtgatcaaga ctgccgcgat cgccggcttg gccgctccat tcatgtccat
     2521 gccgagcatg gccggagccc agaccacgta caagctggcc ttctcggtgc cgggcctcaa
     2581 tttccccttc ttcgtccaca tgatggacct cgccaagaag cacgtgcagg agaagtatcc
     2641 agacgtcgag ttcatcgagc tggacggcca ggagaacggc gcgccgagct ccaccaagca
     2701 atccaatgac atcgacgcga tcatcgccca gaaggtgaac gcgcttgtta tcagcccgaa
     2761 cgatgtcgcc gcgctcgcgc cggcggttca gtcggcggtc gatgccggca tgatcgtcgt
     2821 aacggtcgac cggaatgtca ctgatgtcca gacgttggcc cacgtcggcg cggataacgt
     2881 cgagggtggt cgcgagcagg gtcgctacct gatcgagatc ctgccggacg gcggtgacgt
     2941 catcgagctg cagggccagc ccggcgcagc gccggccatc gaccgccaca agggtctcga
     3001 cgagatcatc tcggcgcagg acaaagtcaa gatcgtcgtc agccagaccg cgcagttcgc
     3061 gcgcgcccag gcggtgaccg tgctcgaggc tgccctccag acggaagagg ccaagaacgt
     3121 ggccgcggtc gtctgcgcga acgacgacat ggccttcggc gcgagcgagg cgctttcgga
     3181 cgccggcaag acggttccgg tgcttggttt cgacgccctg ccggaagctc tgcaggcgat
     3241 ccaggccggt acgatggccg cca
//
LOCUS       DASSGX010000055         3819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_444947, whole genome shotgun sequence.
ACCESSION   DASSGX010000055 DASSGX010000000
VERSION     DASSGX010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444947"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="VFI12_00885"
     CDS             complement(<1..562)
                     /locus_tag="VFI12_00885"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HET7054980.1"
                     /translation="MNEQAGRWYELTTWADSEAIESVAELFARYGYNEGVSIEEPFLQ
                     EQDGDNLRIDISRPAVIRTYIPAESYDPATVDRIRDGLWHLGQIRSVGQLTVTERSEE
                     DWASAWKEHYRPLRASERVVIRPPWFDYEAKPDDIVLVLDPGMAFGTGMHPTTRLSLY
                     QIEKYVQPDQSVFDVGTGSGILAIAAA"
     gene            complement(559..1533)
                     /locus_tag="VFI12_00890"
     CDS             complement(559..1533)
                     /locus_tag="VFI12_00890"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HET7054981.1"
                     /translation="MDVILPVAGLGTRLRPQTWSKPKPLVSLAGKTILEHVLDRVLPT
                     HPEKIVFITGYLGHHIEAWAKENIKIPVAFVEQTEMRGQTDAIIRARDISRDDALILF
                     PDMLFEADFSIIEQSDADVIMFTKEVEDPSALGIAVVENGRIVKLIEKPKEVISNLAV
                     IGIYYIRSMTALYEAIDEQMERGIKTKNEYFIADAIQIMIDKGAKVISAPVTLWEDCG
                     NAQSLLSTNRILLARDEDRIETRGNSLVIHPSMVDASAVLERSIIGPFASVGENCRIS
                     DSLVSDSIIEDGASVECSQLTGSIVGFGASVRGRAASVNVGDVSTINL"
     gene            complement(1653..1726)
                     /locus_tag="VFI12_00895"
     tRNA            complement(1653..1726)
                     /locus_tag="VFI12_00895"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1690..1692),aa:Glu,seq:ttc)
     gene            complement(1765..3249)
                     /locus_tag="VFI12_00900"
     CDS             complement(1765..3249)
                     /locus_tag="VFI12_00900"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HET7054982.1"
                     /translation="MTGAFVAAAVMIGFLYGWAIVPLIAAAMLLVWVGRGWTGAAATL
                     VILGAVAGAWRSEALLHHPLDPNWESDSERTGQIASAVVDDGRTQQFVVRLEDGRRLC
                     VRSFSRADLGRGDQLRMSVEPDSQGSVSEGYGASLRSRSCDWSATLNRVTVLKRGSGA
                     LRLVDGVRSAITRQLVGWVPGDRGALLAGLVIGDDSLISNETKDAFKRTGTLHVVAIS
                     GSNLTLLASLLMVSMAWSTRRRMIDIIALGIIWLYVLVGGAGPPTVRAGILATVAAGS
                     RAVGRPAELLSLSMQIAAIQALVWPASVLGLSYRLSTAAIFGVLIATGGRSFRGFWGA
                     VKLVFLTTLIVNLFLLPILPAQSRPSMVQSLVANTLVAPLISLAFVLGVLAVVLGALA
                     PVAGESLAVLAGEINSATIDIVRWVAGWNWLPDILRWSGSDAPRAALVAGATMVLLAT
                     SSDFRRAVRDLRERLPHVDESTGMLVLGSGLGACLSVAVIALFR"
     gene            complement(3246..>3819)
                     /locus_tag="VFI12_00905"
     CDS             complement(3246..>3819)
                     /locus_tag="VFI12_00905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HET7054983.1"
                     /translation="FNLTPVLAVLIGCVTMFVGIGSSLIISQRASDGDIVITSDPRAF
                     EIVVEVRGEVLSPGVYRLPQDARLADLLSAAGGTTGDADLAPHNLARRLNDAEVVIID
                     RAGLATPIVSGTDTEMTAPISISARININTATATQLETLQGIGPALAQRIIDDRTHNG
                     PYRDVAELTRVSGISEKLLAEIRNQITVGP"
BASE COUNT          742 a         1251 c         1160 g          666 t
ORIGIN      
        1 tggccgcggc aatcgccagg atccccgatc cggttccgac gtcgaagacg gattgatccg
       61 gctggacata cttctcgatc tgatacaggc tcagccgcgt ggttgggtgc attccggtgc
      121 cgaacgccat tccggggtcg agcacgagca cgatatcgtc cggcttggcc tcgtagtcga
      181 accacggcgg ccggatcacg acccgctcgc tggcccgcag cggtcggtag tgctccttcc
      241 aggcagacgc ccagtcctct tccgaacgct cggtaacggt cagttgccca actgagcgaa
      301 tctggccgag atgccagagc ccatcccgaa ttcggtcgac cgtcgccggg tcgtagctct
      361 cggccgggat ataggtgcgg ataaccgcag gcctcgagat atcgatgcgc aggttatcgc
      421 cgtcctgctc ctgaaggaac ggctcctcga tcgagacgcc ctcgttgtaa ccgtaccgcg
      481 cgaagagctc cgcgacggac tcgatcgcct cgctatcggc ccaggtcgtc agctcatacc
      541 accggcccgc ctgttcgttc acagattgat tgtgctcacg tcgccgacgt tgaccgacgc
      601 cgcccgtccg cgaacactcg cgccgaaccc gacgatcgag ccggtaagct gcgagcattc
      661 gacgctggcg ccatcctcga tgattgaatc cgagacgaga gagtcggaga tccggcagtt
      721 ctcaccgacg cttgcgaatg ggccgatgat cgagcgttcg agcacggctg atgcgtcgac
      781 catcgaggga tggatgacca gactgttgcc gcgcgtttcg attcggtctt cgtcccgcgc
      841 caagaggatc cggttggtcg agaggagcga ctgtgcgttt ccgcagtcct cccaaagcgt
      901 gaccggcgct gagatcacct tcgcgccctt gtcgatcatg atctggatcg catcggcaat
      961 gaagtactcg ttcttcgtct tgatgccccg ctccatctgc tcgtcgatcg cctcgtagag
     1021 cgccgtcatg gagcggatgt agtagatgcc gatcaccgcc aggttcgaga tcacttcttt
     1081 cggcttctcg atcagcttga ctatcctgcc gttctcgacg accgcgatgc cgagcgccga
     1141 tgggtcctcg acctccttcg tgaacatgat cacgtcggcg tcagactgct caatgatcga
     1201 gaaatcggcc tcgaagagca tgtctgggaa gaggatcagc gcgtcatcgc gggatatgtc
     1261 gcgagcacgg atgatggcgt cggtctgacc acgcatctct gtctgctcga cgaacgcgac
     1321 cgggatcttg atgttctcct tcgcccacgc ttcgatgtgg tgcccaagat aacccgtgat
     1381 gaagacgatc ttctctggat gcgtcggcag gacgcgatcg agcacgtgtt cgaggattgt
     1441 cttgccggcc aaagagacga gcggcttggg cttgctccag gtttgcggtc gcagtctcgt
     1501 accaagcccc gcgacgggaa gaatgacgtc catggggtgt gtgcctcacg tgcaacgaac
     1561 cagccgattc tgcgtgatcc tagcacgcgc atcggggtcg aaagccgcac cgcggcacag
     1621 tttgcgagga gaagcgggtg gggacgaaac ggtaccccca gccggattcg aaccggcgat
     1681 cttcaccttg aaagggtgac gtcctgggcc gctagactat gggggcgcca gccgacgaaa
     1741 ggtaccgcct gcgcagcctg attgtcaacg aaacagggcg atcaccgcga ccgacaggca
     1801 ggctccaagc cccgatccaa gcacgagcat tccggtggat tcatccacgt gcgggagtcg
     1861 ctcacggagg tctctcaccg cccttcgaaa gtcgctgctc gtcgcaagaa gtaccatggt
     1921 tgcgcccgca acgagcgcag cccgaggtgc atccgatcca ctccaccgca ggatgtccgg
     1981 cagccagttc catccagcaa cccaccgcac gatgtcgatc gtcgcgctat tgatttctcc
     2041 cgccagcacg gccaacgact ccccggcaac cggcgccagc gcaccgagaa cgaccgccaa
     2101 caccccaagc acaaacgcga ggctgatcaa cggcgcgacc aatgtgttcg ccaccagcga
     2161 ctggaccatc gaggggcgac tctgcgccgg caggatcggc aagagaaaca ggttgacgat
     2221 cagagtggtc aggaacacca gcttcaccgc tccccagaat ccccgaaatg agcgtccgcc
     2281 cgtagcgatc aacacgccga agatcgccgc cgtcgaaagc cggtaggaca ggccgagcac
     2341 cgaggccggc cagaccagag cctggatcgc ggcgatttgc atcgacaggg acaacagctc
     2401 ggccggccgg ccgactgccc gcgaacccgc cgcgaccgtc gccagaatgc ccgcccggac
     2461 ggtcggcggt cctgctcctc cgacgagcac gtagagccag atgatgccga gcgcgattat
     2521 gtcgatcatt cgccggcgag tggaccacgc catcgagacc atcaggagcg acgcgagcag
     2581 ggtcagattg gaaccactga tcgcgaccac gtgcaatgtg ccggttcgct tgaacgcatc
     2641 cttcgtctca ttcgaaatca gcgaatcgtc gccgatgacc agcccggcga ggagcgcccc
     2701 acggtcaccc gggacccaac cgaccagttg acgtgtgatc gcgctccgta ctccgtcaac
     2761 caaccggagt gcgcccgatc cccgcttgag gacggttacc cggttcaacg ttgcactcca
     2821 gtcgcagctt ctcgaccgca aggacgcacc gtagccctcg gaaacgctcc cctggctgtc
     2881 tggttcgacg ctcatgcgca attgatcgcc ccgtcccaga tcggcccggg aaaaggagcg
     2941 gacgcaaagc cgccggccat cctcgagccg cacgacaaac tgctgcgtcc ggccgtcgtc
     3001 gacgaccgcg cttgcgatct ggccggtcct ttcgctatcc gattcccaat tcggatcgag
     3061 cggatgatgc aggagcgcct cggatcgcca cgcaccggct accgcgccaa gaatgacgag
     3121 cgtggccgcg gcgccggtcc acccccggcc gacccacacc aggagcatcg cggccgcgat
     3181 gagcggaacg atcgcccagc cgtagaggaa accgatcatg acagccgcgg cgacgaaggc
     3241 accggtcacg gcccgacggt gatctggttc cggatttcag ccaggagctt ctccgagata
     3301 ccagagacgc gtgtcagttc cgcgacgtcc cggtacggcc cgttatgggt ccgatcatcg
     3361 ataatccgct gggccagggc cggtccgatg ccttgcaggg tttcaagctg cgtcgcggtc
     3421 gccgtattga tgttgattct ggcgctgatc gatatcggtg cggtcatctc ggtgtcagtg
     3481 ccggaaacga ttggtgtcgc caacccggcg cgatcgatga tcacgacctc cgcatcgttc
     3541 aaccgccgag cgagattgtg tggggcaagg tcggcgtcgc cggtcgtgcc gcccgccgcc
     3601 gagaggaggt cggccaatcg ggcgtcctgt ggcagccggt acacgccagg ggacagtact
     3661 tcgccccgga cctcgaccac gatctcgaat gccctgggat cggacgtgat gacaatgtcg
     3721 ccgtcactcg ctcgttggct gataatcaga ctggagccga tgccgacgaa catggtgaca
     3781 cagccgatga ggacggccag cacgggcgtc agattgaac
//
LOCUS       DASSGX010000056         5579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1315973, whole genome shotgun sequence.
ACCESSION   DASSGX010000056 DASSGX010000000
VERSION     DASSGX010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5579
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1315973"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1420
                     /locus_tag="VFI12_00910"
     CDS             <1..1420
                     /locus_tag="VFI12_00910"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HET7054984.1"
                     /translation="TGKTGVAFVITGPGVTNIATALGQAYTDSSPVLLIASNNPRATI
                     DGMRGSLHDLKDQLGVLRSVTKSTMRVTAAQDAGNAVLGSLAIAQTDRPLPVAVEFPL
                     DVLDEQAAVAPGALVPAGARRQPDGGVVAEAAARLMSAKRTVIYCGGGAVSSAAPEAI
                     ASIAEALNAPVITSIQGKGAIPADHPNYAGSAWGADNAADELIQQADCMLIFGSRMGA
                     QATYDFKMRFPREVIRVDIDPVELTLNVNPTLAIQADSALAATAIAEQLKGHEHHEGG
                     YSPEQIATARKSAEAGSYHAERREWPDAIRAALPRDGILVTDMTQMAYVATATYPVYE
                     PRTFIFPNGYGTLGFGLPAGIGAKIGRPDAAVVSVVGDGGFQYSMGELAVAIQERIGL
                     PIVIFNDNTYSAVKEAQKWERDERYNAVDLVGNPDFQKLAAAYGIPAVKVTAPGDLTS
                     EIRTALGRDLPTIIEVPTEQWV"
     gene            complement(1389..3350)
                     /locus_tag="VFI12_00915"
     CDS             complement(1389..3350)
                     /locus_tag="VFI12_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7054985.1"
                     /translation="MSRFAFRRDWVLPVLAVVALLLWAWYRLDFFNLSRPVVGPAGTI
                     ERLPNALASVDHPFHAARFGMFLDALSHGHVPHWVFSHQGGYPAEFYPFGSSVVDLLV
                     WCLTLGQMSIPMVHTWAVAIVFGLPALGFVGTARLARLPLWIAPLALACHLCVRGWWW
                     SGSSMELIEWGLVTNVLAAALTFLALVAIATGLDRHSPRWMMVGAGLIAWAEFTNPRS
                     LIAIGTIAVAGLIVWLLQSRGERVAFGWLLAPFVLGIAGTAPLLISLIRYNSLYYFVH
                     YSGYANLREWLDSSVQAVSGPIFVVALAGLTIAIGGNRSPAESLIGWTTVVYGALTAW
                     LVEVDWPARWTEQLETTRLMPFQRLLMIALAAIAVGRLAQWLIPRWSAVAIAGLTALV
                     PILYVLGPPAFIPESDRGLVRIGTMAAPGIADLKAAVDVADTSTPPDTAILILGTTVS
                     WHDQMWATLWSERLFFYDDWLWYWQREHVGDYNPNTEHAYPVDSSTINRAYLSTHGIG
                     AIVLTGEAKGAAADASFLKLVRTGIYDVYQVTGGTTTATLSGANVLSRIDDESIVVSG
                     IGAGGTVTVRENWFPRWRATVGGEDVPVVHRPDGYMDVPVPAGSHALVLRYQATALDW
                     ITRAVAFLAAVIAIAILVRARPTAPSEPR"
     gene            complement(3347..3697)
                     /locus_tag="VFI12_00920"
     CDS             complement(3347..3697)
                     /locus_tag="VFI12_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7054986.1"
                     /translation="MMHEQPTAKHRDLRLLWCALVVMIGAALMLLSVPTTLGVKVPET
                     RIPGLDPSLIDTSSHEHVWRVWWRARDDYLTVHPQWILSAGLLVLLVVFVIGVLAAIW
                     FALSPVAEADDAAE"
     gene            complement(3694..5190)
                     /locus_tag="VFI12_00925"
     CDS             complement(3694..5190)
                     /locus_tag="VFI12_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7054987.1"
                     /translation="MSRSRAIALSGIRAWPAHRKYIVVLLLLFAVKQAFNVIVFPPFT
                     GHDEVAHYAYIRTVADEHRVPVIPDLAEWRAAWQEHKTTFPGDYLPLDLYKYQRYVLD
                     WCCSASDRPEFATQPPTAMSWAGETYPNGWQYTANHPPLYYAVMAPIYKATDSLSPES
                     QQYVLRAAAIPIGLLVVLLTYLIAGLLFPGDRFMMTVPPTFVAFQTQVSYEAAMVNND
                     ILLIGLFTLLIYLLIRGVRCGFTLQSSAAIGLVLGLGLLAKASMLTAAPLVAIAIVFG
                     VGIRHVRRWVTLGATSAVVAALVCWPWYVFLYRTYGNLSGLAQIKALQYQWTYRFQTP
                     PTIFDELFSLNFARMRWKETWGEFGWRLIHLDGWLLAVIGIPLLIMAVAAIVSLVVAS
                     VRRLSADSVNRPTLDRAQVRGLWLLLTVCVIAYGAMLQFGTDFSLTQARYYFNAIAAV
                     AILIAFGLRQVVPARYHSTAIVVFLLFFVTVNVLIYTQYVLPYWYLAS"
     gene            complement(5290..>5579)
                     /locus_tag="VFI12_00930"
     CDS             complement(5290..>5579)
                     /locus_tag="VFI12_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7054988.1"
                     /translation="IGGFTIRAPRAYPSYVMGYEKHLEKVKDFVYSLENIQIIGRYGT
                     FRYNNTDHSIETGLLAAKNILGEHHDLDEVNAEKEYHEMKRVEPALAGSRR"
BASE COUNT         1126 a         1832 c         1753 g          868 t
ORIGIN      
        1 cacgggcaag acaggcgtcg ccttcgtcat caccggaccc ggcgtcacca atattgcgac
       61 cgcgctgggg caggcctaca ccgattcctc gcccgtcctc ctgattgcct cgaacaatcc
      121 gcgcgcgacg atcgacggga tgcgcggctc gcttcacgac ctgaaggacc agctcggcgt
      181 gctccgctcg gtcaccaagt cgaccatgcg ggtcacggcc gcccaggacg ccggcaacgc
      241 agtcctcggc tcgctcgcga tcgcccagac cgatcgacca cttcccgttg cggtcgagtt
      301 cccgctcgac gtgctcgatg agcaggccgc ggtcgcgccc ggcgcactgg tgccggccgg
      361 cgcccgacgc cagcctgacg ggggcgtcgt tgccgaagcg gcagcgcggt taatgtcggc
      421 caagcggacg gtcatctatt gcggtggcgg cgccgtctca tcggccgccc cagaggcgat
      481 tgcctcgatt gccgaggcgc tcaatgcgcc ggtcattacg tcaatccagg gcaagggcgc
      541 gatcccggcc gaccacccga actacgccgg cagcgcgtgg ggcgcggaca acgcggccga
      601 cgagctgatt cagcaagccg attgcatgct gatctttggt tcgcggatgg gtgcgcaggc
      661 gacgtacgac ttcaagatga ggttcccgag agaggtgatc cgcgtcgaca tcgatccggt
      721 cgaactgacc ctgaatgtga acccgacgct cgccatccag gccgattccg ccctggccgc
      781 gaccgcaatc gccgagcagc tcaagggaca cgagcatcac gagggcgggt acagccccga
      841 gcagatcgcg actgcccgca agagcgccga ggccggctcc tatcacgccg agcggcgcga
      901 gtggccggat gccatccggg ccgccttgcc gcgggacggg attctcgtca ccgacatgac
      961 gcagatggcc tacgtggcga ccgcgaccta cccggtctat gagccacgca ccttcatctt
     1021 cccgaacggc tacggcacgc tcggtttcgg actcccggcc ggaatcggcg cgaagatcgg
     1081 ccggccggat gcagcggtcg tctcagtggt cggcgacggt ggcttccagt actcaatggg
     1141 cgaactggcc gtggcgatcc aggagcggat cggcctgccg atcgtgatct tcaacgacaa
     1201 cacctactcg gcggtcaagg aagcgcaaaa gtgggaacgc gacgagcgat acaacgccgt
     1261 cgacctcgtc ggcaacccgg atttccagaa gctggccgct gcctacggca ttccggcggt
     1321 caaggtgacc gcgcccggcg acctgacgtc ggagattcgg acggcgctcg gccgcgacct
     1381 cccgacgatc atcgaggttc cgacggagca gtgggtctag cgcggacgag aatcgcgatc
     1441 gcgatcaccg ccgccaggaa cgcgaccgcg cgggttatcc agtccagcgc ggtcgcctga
     1501 tatcgcagca ccagcgcatg ggagccggcc ggcacgggta cgtccatgta gccgtccggc
     1561 cggtgcacga cgggcacgtc ttcgccgccg acggtagccc gccagcgcgg gaaccagttc
     1621 tcgcggaccg tgaccgtccc tcccgctccg atcccgctga cgacgatcga ttcgtcgtcg
     1681 atgcgggaca acacattcgc accgcttaag gttgcggtgg tcgtcccacc ggtcacctgg
     1741 tacacgtcgt agatccctgt tcgcaccaat ttcaggaagg atgcgtccgc cgcagcgccc
     1801 ttggcttcgc cagtcaatac aatcgcgcca atcccgtgtg tcgaaaggta ggcccggttg
     1861 attgtcgagc tgtcgacggg ataggcatgc tcggtattcg ggttgtagtc gccaacgtgc
     1921 tcgcgctgcc agtaccagag ccagtcgtcg tagaagaaga gccgttcgct ccagagcgtc
     1981 gcccacatct gatcgtgcca ggagaccgtc gtaccgagga tcaggattgc cgtgtcaggc
     2041 ggagtcgacg tgtcggcaac atcgactgcc gccttaaggt cggcgattcc aggcgcggcc
     2101 atcgtcccga tgcgaacaag cccgcgatcc gattcgggga tgaacgcggg tgggcccagg
     2161 acgtacagga tcggtaccag cgccgtcaga ccggcaatcg cgaccgctga ccagcgcgga
     2221 atgagccatt gcgcaagccg gcctaccgca atcgctgcca gagcgatcat cagcagccgc
     2281 tggaacggca tcagccgggt tgtctcgagc tgctcggtcc agcgcgcggg ccaatcaacc
     2341 tcgaccagcc atgcggtcaa tgcgccgtag acaacggtcg tccaaccgat caacgattcg
     2401 gcaggcgagc ggttaccgcc gatggcgatg gtcaggccgg caagcgccac gacgaagatc
     2461 ggaccagaga ccgcctgcac cgacgaatca agccactccc gcagattcgc gtatccgctg
     2521 taatggacga agtaatagag gctgttgtag cgaatcagcg agatcagcag cggcgctgtg
     2581 ccggcgatcc caaggacaaa gggcgcgagc agccagccga atgcgacgcg ctcacctctt
     2641 gattggagca gccagacgat cagcccggcg acggcgatcg tcccgatagc gatcaacgac
     2701 cgcgggttcg tgaactccgc ccatgcgatc aatccagcgc cgaccatcat ccagcgcggg
     2761 ctgtggcgat ccaacccggt cgcgattgcg accaatgcca ggaaggtcag ggcggcggcc
     2821 agcacgttcg tgaccagccc ccactcgatc agctccatcg agcttccgga ccaccaccag
     2881 ccccgcacac agaggtggca ggccaatgcc agcggcgcga tccatagcgg caagcgcgcc
     2941 aggcgggcgg tccccacaaa gccgagcgcc ggcaggccaa agacgatcgc gacggcccag
     3001 gtatgaacca tcgggatgga catctgcccc agcgtcaggc accagaccag caggtccacg
     3061 acgctgctgc cgaacgggta aaactcggcc ggatacccac cctggtggct aaagacccag
     3121 tgcggcacat gtccgtgact cagcgcatcc aggaacatgc cgaagcgggc cgcgtggaac
     3181 gggtgatcga ccgatgcgag cgcgttcggc agtcgctcga tcgtgccggc cggcccgacg
     3241 accggtcgag acagattaaa gaaatcgagc cgataccacg cccaaagcag cagggcgact
     3301 acggcaagga ccgggaggac ccagtcgcgt cggaaagcga accggctcat tcggccgcgt
     3361 cgtccgcctc cgcaacgggc gacagcgcga accagatcgc ggcgaggacc ccgatcacga
     3421 acactaccag caagaccagc agccccgcac tcaggatcca ctggggatgg acggtgagat
     3481 agtcgtcccg cgcccgccac cagacccgcc agacatgctc atggctcgac gtgtcgatca
     3541 gcgatgggtc gagaccggga atgcgggtct ccggcacttt cacaccgagg gtggtcggga
     3601 cgctgagcag catcagggct gcgccgatca tcacaacgag cgcgcaccag agcaaccgaa
     3661 gatctctgtg cttcgcggtt ggctgctcgt gcatcatgac gccaggtacc agtagggcag
     3721 cacgtactgg gtgtagatca gcacattgac agtcacgaag aacagcagga aaacgacgat
     3781 cgccgtggag tggtagcgag ccggcacgac ctgtcgcagc ccgaatgcaa tcaggatcgc
     3841 gaccgccgcg atcgcgttga agtagtaccg cgcctgcgtc aacgaaaagt cggtaccgaa
     3901 ctggagcatc gcgccatacg cgatcacgca gaccgtcagc agcagccaca ggccgcgcac
     3961 ttgcgcccga tcgagtgtgg gtctattcac gctgtcagcg gagaggcgtc gaacgctcgc
     4021 cacgaccagg gacacgattg cggcaacagc catgatcaag agcggaatgc cgatcacggc
     4081 gagcagccaa ccatcgaggt ggatcaggcg ccacccgaac tcgccccacg tctctttcca
     4141 gcgcatcctg gcgaagttca ggctgaagag ctcatcgaaa atcgtcggcg gcgtctggaa
     4201 ccggtacgtc cactggtact gcagcgcctt gatctgtgcc aggccgctca ggttgccgta
     4261 ggtccggtac aagaacacat accacggcca gcagaccagc gccgcaacca cagctgacgt
     4321 ggcgccgagc gtcacccacc gtcgtacatg gcgaatcccg accccgaaga cgatcgcgat
     4381 ggcaaccagc ggcgcggcgg tcagcatcga tgccttcgcc agcaagccga gacccagcac
     4441 gaggccgatc gccgcgctcg attgcagcgt gaacccgcac cggacgcctc gtatcagcag
     4501 ataaatcagg agtgtgaaca gtccgatcag caggatgtcg ttattgacca tcgcggcctc
     4561 atacgagacc tgcgtctgga acgcgacgaa ggtcggcggg acggtcatca tgaagcggtc
     4621 gcccgggaag agcaggccgg caatcaggta agtgagcaag acgaccaaca acccgatcgg
     4681 aattgcggct gcgcgcagca cgtactgctg gctctcgggg gagagtgaat cagtcgcctt
     4741 atagatcggc gccatcactg catagtaaag cggcggatga tttgcggtgt actgccagcc
     4801 gttcggatac gtttcccctg cccaggacat tgcggtaggc ggctgcgttg caaactcggg
     4861 ccggtccgac gcgctgcaac accagtcaag tacataccgc tggtacttgt aaaggtccaa
     4921 cggcaagtag tcgcccggaa acgtagtctt gtgctcctgc caagcagcgc gccattcggc
     4981 gagatcaggg atcaccggaa cccggtgttc gtccgcgacc gtgcggatgt acgcgtagtg
     5041 cgcgacttcg tcgtgcccag tgaatggcgg gaagacgatg acgttgaatg cctgcttgac
     5101 cgcgaagagg agcagcagga ccacaatgta cttccgatgc gccggccagg cgcggatgcc
     5161 gctcaacgcg attgcacgcg acctgctcac tgcgagcgtg cctgttgccg gcgctatcgt
     5221 tgcttgatca agcacttcag cacgctccgc caacaggcac tccccgtctc agattcgccg
     5281 tactgctgcc taccgccggc tgccggccag cgcgggctcg acccgcttca tctcgtgata
     5341 ttccttctcg gcattgacct catcgaggtc atggtgctcg ccgaggatgt tcttggccgc
     5401 caggagaccg gtctcgatcg agtggtcggt gttgttgtac cggaatgtcc cgtaccggcc
     5461 gatgatctgg atattctcga ggctgtagac gaaatctttc accttctcga ggtgcttctc
     5521 atagcccatc acgtacgagg ggtacgcccg cggcgcgcgg atagtgaagc cgccgatca
//
LOCUS       DASSGX010000057         3082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1425370, whole genome shotgun sequence.
ACCESSION   DASSGX010000057 DASSGX010000000
VERSION     DASSGX010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1425370"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(234..950)
                     /locus_tag="VFI12_00935"
     CDS             complement(234..950)
                     /locus_tag="VFI12_00935"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET7054989.1"
                     /translation="MRVAVLSDIHGFSLALETVRADIAEQGPFDEIVVAGDLCVVGPD
                     PQGVVDTIRAEGWTAIQGNTDFDLVEAAEWATGIEQLGYWVEHLTEDAMDFVAELPFS
                     HRIMPPGGTSPAHELLIVHANPHNLTDVLDPRAMDRELREIIGGEDAGTIVFGHIHIA
                     YIRHVAGTMLVDVSAVGNPKDGDLRPRYGILNWDANTQRWTAEIRRLPYPLEATEAQI
                     RASDLPDPEALIDSLKRASY"
     gene            1145..>3082
                     /locus_tag="VFI12_00940"
     CDS             1145..>3082
                     /locus_tag="VFI12_00940"
                     /inference="COORDINATES: protein motif:HMM:NF026288.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7054990.1"
                     /translation="MPQPLTPLIGRGSELAGVRDLLDRPDVHLVTLTGPGGAGKTRLS
                     IEMGYLERPNFSDGVVFVPLANLTSSNAMLGAIAQALNIREGSGESLAGAIQAVAADL
                     ELLLILDNLEQIDNPVPALTLLFDNAPAIRILATSRSALRIRGEHEVQVEPFATPNPA
                     KMPGLDDLVLNPAVALFCDRASAVRPTFVLNQDNAADVVEICRRLDGLPLAIELAAAR
                     SKLLTPSQMLPRLANRLQLLTGGARDLPERQQTMRDAVSWSYDLLPDREQRLFRRLAV
                     FAGGATIDAINAVAGEGGEDLFAELAALVDHSLVRAFEGSDGDARYAMLLTIRDYAAE
                     LLADTGEESTIRGRLITHYVALSDTIKSELVGAGSAHWLKVVDAEAPNLRAGHRWAIE
                     HGDVSSAQQIASALPRYWEVQGNYTEGRSWLAAALAVDANPTVERAHALIGLATLARR
                     QGDYDAATSAYQEGLAIFREHDDQSGVATTLNNLGVVAQDKGDYDRARELLAEAQRYF
                     ASIKDRPRNASALNNLGLVARRRGDLKGAVDLYEQSLAIWTELGDQLRRALCMNNLGV
                     VAYALGDNQTAENRYRDALTVYRQREDRSGAAQTLHNLAEILRDRGDYPEAISAWQES
                     LALRSVQGDRVGIAECLTGLAR"
BASE COUNT          529 a         1037 c          984 g          532 t
ORIGIN      
        1 ggggcggctt gccccgcatc gcccacaggg cgatgaccgg tgtcggccaa tcacgttgcc
       61 gggccgagac cggccactgg acgacatagt cgtggcctgc ggccacagcg gagcgagccg
      121 ctccgcgcta cgtttaccag cgctgcaccc gccggattca tcagcgagga aataatcgtc
      181 ccgcgggcga tagcggggcg tggtaatccc gcgctacgtg tcgctctaca gcattaatac
      241 gaagctctct tgagggaatc aatcagggcc tcggggtcgg gcaggtcgct ggcgcggatt
      301 tgcgcttcgg ttgcttcgag tgggtatggc aagcgccgga tttcggctgt ccaccgctgc
      361 gtgttcgcgt cccagttcag gatgccgtag cgaggccgga gatcgccgtc cttcggattg
      421 ccgaccgccg agacgtcaac cagcatcgtg ccggcgacgt ggcgaatgta ggcgatatgg
      481 atatggccga agacgatcgt cccggcgtcc tcgccgccga tgatctcgcg gagctcgcgg
      541 tccatcgcgc gcggatcgag cacgtcggtc aggttgtgcg ggtttgcatg gacgattagc
      601 agctcgtgcg cgggactcgt cccgcccggc ggcatgatcc ggtggctgaa gggcagctca
      661 gcgacgaaat ccatcgcgtc ctcggtcagg tgctcgaccc agtagccgag ctgttcgatc
      721 ccggtcgccc actccgctgc ctcgaccagg tcgaaatcgg tgttgccctg aatcgcggtc
      781 cagccctcgg cccggatcgt gtcgactacg ccctggggat cagggccgac gacacagagg
      841 tcgccggcga cgacgatctc gtcgaagggt ccctgttctg ctatatccgc gcgcacagtc
      901 tccagcgcca ggctgaaccc gtgaatatca gacagtaccg cgacccgcat ccagccctcc
      961 gctcgccatg tttcgctccc agccacaggg agcaagaccc tgctgcattg atcctacatt
     1021 gaagcggtga tttgacgata ggtaggcggc ggggcatgat caagccgact ggtgcattcg
     1081 caactcgaaa gacggtgtac ccccgaccgt gtgcgcgacg accaaacccc ggccgatctt
     1141 cagcatgccg cagcccctga cgcctctgat cgggcgcgga tccgaacttg ccggcgtgcg
     1201 ggatctgctc gatcgcccgg atgttcacct cgtcacgctg accggtccgg gcggggctgg
     1261 caagacgcgg ctctcgattg aaatgggcta cctcgagcgg cccaacttca gcgatggcgt
     1321 cgtcttcgtg ccactcgcga acctgacttc atcgaatgcg atgctcggcg ccatcgccca
     1381 ggccctgaac atccgcgagg ggtccggcga gtcgctggcc ggcgcgatcc aggccgtcgc
     1441 ggccgacctg gagctgctgc tgatcctcga caacctcgaa cagatcgata acccggtccc
     1501 ggcactgacg ttgctcttcg acaacgcgcc ggcaatccgg attctggcca cgagccggtc
     1561 tgccctccgg atccggggtg agcatgaggt gcaggtcgag ccattcgcga caccgaaccc
     1621 cgcgaaaatg ccggggctgg acgatttagt cctgaacccg gccgtcgcgc tcttctgtga
     1681 ccgagcctct gccgttcgcc caactttcgt gctcaaccag gacaacgcgg cggacgtggt
     1741 cgaaatttgc cggcgcctgg atggcctgcc actcgcaatc gagctcgccg cggcgcgatc
     1801 caagctgctg actccatctc agatgctgcc ccgcctcgct aaccggctcc agctcttgac
     1861 cggcggcgcc cgggacctcc ccgaacggca gcagaccatg cgtgatgccg tctcatggag
     1921 ctatgaccta ctcccggaca gggagcagcg gctcttccgg cgcttggcgg tctttgccgg
     1981 cggagccacc atcgatgcca tcaatgcggt tgccggcgag ggtggtgagg acctcttcgc
     2041 cgagttggcc gcgctcgtcg accacagtct ggtgcgcgca ttcgaggggt ccgacggaga
     2101 tgcccgatac gccatgctcc tgacgattcg cgactatgct gccgagctgc tcgcggatac
     2161 cggtgaggaa tccacgattc gcggtcgcct gatcactcat tatgtcgcgc tgagcgacac
     2221 aatcaagagc gagctggttg gcgccggctc ggcccactgg ctcaaggtcg tggacgctga
     2281 ggctccgaac ctgcgcgctg gtcatcgctg ggccatcgag cacggcgacg tgagttccgc
     2341 ccagcagatc gccagtgccc tgccccgcta ttgggaggtc cagggcaact acaccgaagg
     2401 ccggtcctgg ctcgctgcgg cgctagccgt cgatgccaat ccgaccgtcg agcgggcgca
     2461 tgccctgatc ggcctcgcca cgctggcccg ccgccagggc gattacgacg cggcgacatc
     2521 ggcctatcag gaaggtctgg cgatcttccg ggaacacgac gaccagtccg gcgtggcgac
     2581 cacgctcaac aacctcggcg tcgtcgcgca ggacaagggc gattacgatc gcgcccggga
     2641 gttgctcgcg gaagcacagc gctacttcgc atctatcaaa gaccggccac gcaatgcctc
     2701 ggccctgaat aaccttggat tggtcgcgcg gcgacgaggc gatctgaagg gtgcggtcga
     2761 cctctacgag cagagcctcg ccatctggac cgagctcggc gatcagcttc gccgcgcgct
     2821 ctgcatgaac aatctcggtg tggtcgccta cgccctcggt gacaaccaga cagccgaaaa
     2881 ccgctatcgc gacgcgctga ccgtctaccg gcaacgcgag gaccgcagcg gggcggcaca
     2941 aaccctgcac aacctggccg aaatcctgcg cgatcggggc gattacccgg aggcgatctc
     3001 ggcctggcaa gaaagcctgg cgcttcgctc ggtgcagggc gaccgggttg gcatcgccga
     3061 gtgcctgacc ggcctggccc gc
//
LOCUS       DASSGX010000058         2799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_445750, whole genome shotgun sequence.
ACCESSION   DASSGX010000058 DASSGX010000000
VERSION     DASSGX010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445750"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VFI12_00945"
     CDS             complement(<1..482)
                     /locus_tag="VFI12_00945"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET7054991.1"
                     /translation="MAQKLTFGVVVRPQHWPWADQISEWPKLEAMGFDSIWLNDHFHS
                     LGDTIDNPAFEASTTLAALALSTSRVRIGVLTYGNTHRNPAILAKQIVTVDQMSGGRV
                     IFGIGTGWHESEHAAFGIALPSAGERVRRLEETLEMFRMLEQNDRTTYHGRYYTLEDA
                     P"
     gene            complement(485..1345)
                     /locus_tag="VFI12_00950"
     CDS             complement(485..1345)
                     /locus_tag="VFI12_00950"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET7054992.1"
                     /translation="MAHPLTFGVVIEPQYWDWASNVELWKEAEALGFESIWLDDHFYP
                     VGDSNDREGFEASTTLAALALTTTKPKIGVLTYGNTHRNPAILYKEMVTVDHMSGGGR
                     LIFGIGAGWNEPEHRAYGIQFPSAGDRVAMLDEALDLIHEFEKNDRTTFHGRYYSAED
                     APFVPKPVNGHIPLLIGGKRPKMLRVIAKHADYWDSGLEADELKIALQTIRQHAAELG
                     RDPDSITASTPVWRHQMSDREFADRVRAFYAAGVRQMLIQHPGDRKGIETIPGLMEHV
                     VPELRAELEG"
     gene            complement(1376..1996)
                     /locus_tag="VFI12_00955"
     CDS             complement(1376..1996)
                     /locus_tag="VFI12_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7054993.1"
                     /translation="MIRQCSRWFPATLLVLAIVIVFGILTADNTLGTHDRLMDRIGMD
                     LPSLRHVRLWTMPLATFVQSEPGVAWSMLLLVGLPMIALEFLAGSWRALVTFLLSDWL
                     TAPVTVLILWALSGLGDAGASRLLADPDTGSSAAAHGAIAAAIMLLPPKLAAIGMAIL
                     IGITAILMAHYRLDTSIAHVLGTAVGAGFGWLWARQRQRNPAYHDA"
     gene            complement(1993..>2799)
                     /locus_tag="VFI12_00960"
     CDS             complement(1993..>2799)
                     /locus_tag="VFI12_00960"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HET7054994.1"
                     /translation="EASFQQIVTEFLDFCDANAWRAAFHQVGPEHLPEYRSAGLVSLK
                     IGEDAIVDLRDWSLSGSVMKPLRGALNRVQRAGHTICFHAPPLPNGLVAELRDVSCEW
                     LRLPGRRERGFTLGQWDDAYIRRSPVFTVTSSSGRIVAFINLVRDGVPGEGTFDLMRH
                     RPDAPNGAMDALLVRLAEHLHDQGCTRLSLGMVPFADVGTDEEDPVLERALRLLVERM
                     ERFFSYRGLRLYKEKFHPIWEPRYLVYQSGVALPQIVLAIIQLTERESPG"
BASE COUNT          495 a          973 c          872 g          459 t
ORIGIN      
        1 ggcgcatcct cgagcgtgta ataccgcccg tggtaggtcg tccggtcgtt ctgctccagc
       61 atccggaaca tctcgagcgt ctcctcgagc cgccgcaccc gctccccggc cgagggcaac
      121 gcgatcccaa atgccgcgtg ctccgattcg tgccagccgg tcccgatccc gaagatcacg
      181 cggccgccgc tcatctggtc gaccgtcacg atctgcttgg ccaggatcgc cgggtttcgg
      241 tgcgtgttgc cgtaggtcag cacgccgatc cggacccggc tagtcgagag cgccagcgcc
      301 gccagcgtgg tcgaggcctc gaaggccggg ttgtcgatcg tgtcgccgag cgagtggaaa
      361 tggtcgttga gccagatcga gtcgaagccc atcgcctcca gcttcggcca ctccgagatc
      421 tggtccgccc acggccagtg ctgcggccgg accactacgc cgaaggtcaa cttctgtgcc
      481 acgctcagcc ctccagctcg gcacgcagct cggggacgac gtgctccatc agccccggga
      541 tcgtctcgat ccccttgcga tcgcccggat gctggatcag catttgccgc acaccggccg
      601 cgtagaacgc ccgcacccgg tccgcgaact ccctgtcgga catttgatgc cgccagaccg
      661 gcgtcgaggc agtgatcgaa tcgggatcgc gcccgagctc ggcagcgtgc tggcgaatcg
      721 tctggagggc gatcttcagc tcgtctgctt ccagtccgct gtcccagtaa tcggcgtgtt
      781 tcgcgatcac gcggagcatc ttcggccgct tgccgccgat cagaagcggg atgtgcccgt
      841 tgaccggctt cggcacgaag ggcgcgtcct cggccgagta atagcgaccg tggaaggttg
      901 tgcggtcgtt cttctcgaac tcgtggatca gatcgagcgc ctcgtcgagc atcgcaaccc
      961 ggtccccggc gctcggaaac tgaatgccgt acgcgcgatg ctccggctca ttccagcccg
     1021 cgccgatacc aaagatcagc cgcccaccac cgctcatgtg gtcgacggtc accatctcct
     1081 tgtagaggat cgccgggttc cggtgcgtgt tgccataggt gaggacgccg atcttcggct
     1141 tggtcgtcgt cagggcgagc gcggcgagcg tcgttgacgc ttcgaacccc tcgcggtcgt
     1201 tgctatcgcc caccgggtag aagtgatcat cgagccagat cgactcaaag ccaagtgctt
     1261 ccgcttcctt ccacagctca acattcgatg cccagtccca gtactgcggc tcaatcacta
     1321 caccaaaggt caatgggtgt gccacctgcg cctcccggcc acttgcccga atcagtcagg
     1381 cgtcatggta cgcggggttg cgttgtcgct gccgggccca gagccagccg aatccggcgc
     1441 cgaccgcggt cccgaggaca tgcgcgatcg aggtgtcgag ccggtagtgg gccatcagaa
     1501 tggccgtgat cccgatcagg atggccatcc cgatcgccgc cagcttcggc ggcaggagca
     1561 tgattgccgc cgcgatcgct ccatgcgcgg cagccgagga gccggtatcg ggatcagcca
     1621 gcagccgact ggcccccgca tcgccgaggc cggacagcgc ccacaggatc agcacggtca
     1681 ctggcgcggt cagccagtcg ctgagcagga aggtcaccag cgcccgccag ctcccggcca
     1741 ggaactcgag cgcgatcatc ggcaacccga ccaggagcag catcgaccac gccacgccgg
     1801 gctcggactg cacgaaggtc gcgagcggca tagtccagag ccggacgtgc cgcagcgatg
     1861 gcaggtccat cccgatccgg tccatcagcc ggtcgtgggt acccagcgtg ttgtccgcgg
     1921 tcaggatccc gaagacgatc acgatcgcga gcacgagcag ggtcgccggg aaccagcggc
     1981 tgcactgccg tatcaacccg gcgattcgcg ctcggtaagc tggatgatcg ccagcacgat
     2041 ctgcggcagg gccactccgg actggtaaac gaggtaccgc ggttcccata tcgggtgaaa
     2101 tttctccttg tagaggcgca atccgcggta cgagaagaac cgctccatcc gctccaccag
     2161 taaccgcaaa gcgcgctcca gtaccgggtc ttcctcgtcc gtgccgacat cggcaaacgg
     2221 gaccatcccg agcgacaacc gggtgcatcc ctgatcgtgg agatgctcgg ccagccggac
     2281 cagcagcgcg tccatggcgc cgttcggggc gtccggccgg tgccgcatca ggtcgaaggt
     2341 gccctcgccc ggcacgccgt cccggaccag attgatgaac gccacgatcc ggcccgaaga
     2401 tgaggtcacg gtgaacacgg ggctacgccg gatgtaggcg tcgtcccact gcccgagcgt
     2461 gaagccacgc tcgcgtcggc ccggcagccg caaccactcg caggagacgt cccgcagctc
     2521 ggccaccaag ccattcggaa gcggcggcgc gtggaagcag atcgtatggc ccgcgcgctg
     2581 gacacgattg agcgcccccc gcaacggttt catcacactg ccgctcagac tccaatccct
     2641 caggtcgacg atcgcgtcct caccgatctt gagtgaaacg agacccgccg accggtattc
     2701 cggcaagtgc tccggcccga cctggtggaa cgccgcccgc caagcattgg catcgcagaa
     2761 gtccaggaac tcggtcacga tctgctggaa tgacgcctc
//
LOCUS       DASSGX010000059         3631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1207158, whole genome shotgun sequence.
ACCESSION   DASSGX010000059 DASSGX010000000
VERSION     DASSGX010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207158"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /gene="miaA"
                     /locus_tag="VFI12_00965"
     CDS             <1..738
                     /gene="miaA"
                     /locus_tag="VFI12_00965"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HET7054995.1"
                     /translation="YGFVPAHEAYSVGRYIEDAARAVASAEAEGRLPIFVGGTGLYFK
                     ALLEGLSPIPAIPPDIRTRWRTEARRIGAAALHVVLRARDPEMAERLRPSDPHRIVRA
                     LEVHEATGRSLSEWQRISGKGVLEEAETARLVLLPDRADLYRRCEERFDAMLEQGALD
                     EVAALRELRLDAGLPVMTAHGMPHLTAALAGKLDLATAAARSKQDTRHYAKRQLTWIR
                     RNMKSWTLLKTQELENIHGQIDIIVNV"
     gene            850..2616
                     /locus_tag="VFI12_00970"
     CDS             850..2616
                     /locus_tag="VFI12_00970"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF006581.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HET7054996.1"
                     /translation="MARPMTGAEMVITALQDQGVEFIFGYPGGAVLPIYDEIFQQENV
                     KHVLVRQEGGAVHAAEGYARSTGRVGVVLVTSGPGATNAVTGLTDALMDSIPVVCITG
                     QVPTHLIGSDAFQECDTVGITRSCTKHNWLVKDVNDLARILHEAFYVARTGRPGPVVV
                     DIPKDVQFATGNYVGPSNIAHKTYKPRVKPETAGVREAIELMATAKKPVFYTGGGVIN
                     SGPKASQLLRELVRLTGFPITSTLMGLGAYPASDKQWLGMLGMHGTYESNMAMHDCDV
                     MIAVGARFDDRITGRIDAFSPSSKIIHIDIDPSSINKNVHAHVPIIGDVAYVLEDMVG
                     LWKQRAPQVDKTAHKAWWKQIDQWRTRKSLSYKASADVIMPQYAIERLYALTKDRDVY
                     ITTDVGQHQMWAAQFFRFEEPNRMMTSGGLGTMGYGLPAAIGAQIAHPKSLVVSVSGD
                     ASILMNIQEMSTAVQYRLPVKAFILNNQYMGMVRQWQQLLHGNRISESYTEALPDFVK
                     LAEAYGGIGIRCDRPADLDDAIEEMIRTPKPVLFDCRVAALANCFPMIPSGKAHNEML
                     LGEEVSDEEIGSVIGERGKVLV"
     gene            2700..2969
                     /locus_tag="VFI12_00975"
     CDS             2700..2969
                     /locus_tag="VFI12_00975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HET7054997.1"
                     /translation="METTRLSSKGQVIIPKAVREARGWEPGTELVVEEHADGVLLRSR
                     PAFAATRIDDVAGCLKRHGPPKSVREMDEAVAAEARRRWRKFEAQ"
     gene            2966..3364
                     /locus_tag="VFI12_00980"
     CDS             2966..3364
                     /locus_tag="VFI12_00980"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HET7054998.1"
                     /translation="MIAIDTNVLVRFLVGDDPEQLERAAALVRRNEIFVGLTVLLETE
                     WVLRSAYGATRDEIVVAFRGVAGLRNVNVEAPAILMDAINALEQGMDFADALHLAGAA
                     HASAAAFATFDEDLRKRAGALGRQVRVTGL"
     gene            3376..>3631
                     /locus_tag="VFI12_00985"
     CDS             3376..>3631
                     /locus_tag="VFI12_00985"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HET7054999.1"
                     /translation="MHLTIANKLYSSWSLRPWLVMTAFGIPFEETVIPMYQPDSKARM
                     RAASPTGKMPCLVDGDVTVWESLAIIEHIAERFPEKPIWPT"
BASE COUNT          659 a         1149 c         1200 g          623 t
ORIGIN      
        1 tacgggttcg tgccggcgca cgaggcctat tccgtcggcc gttacatcga ggatgcagcc
       61 cgcgccgtcg cttccgccga ggcggagggg cggctgccga tcttcgtcgg cggcactggg
      121 ctctacttca aggcgctgct cgaagggctt tcgccgatcc ccgccattcc gcctgacatt
      181 cgcacgcgct ggcgcaccga ggcgcggcgc atcggggctg cggcgctgca cgtggtgctg
      241 cgcgcgcgtg atcccgagat ggccgagcgg ctgcggccga gcgatccgca ccggatcgtg
      301 cgggcgctcg aggtacacga ggccacgggc cgttccctca gcgaatggca gcgcatctca
      361 ggcaagggcg tgctggagga ggccgagacg gctcgcctgg tgctgctgcc ggatcgcgcc
      421 gacctctacc gccgctgcga ggagcggttc gacgccatgc tggagcaggg ggccctcgac
      481 gaggtggctg cattgaggga gctgcggctc gatgccggcc tgcccgtcat gaccgctcac
      541 ggcatgccgc atctcacggc agcgctcgcc gggaagctcg atctcgccac cgctgcagca
      601 cggtcgaaac aggacacgcg acactatgcc aagcggcagc tgacatggat cagacgcaat
      661 atgaagtcct ggacgctgct caagacgcaa gaattggaaa atattcacgg acaaatcgac
      721 ataattgtaa atgtctgatt gttgaccatc actcatggcg gagctagggt gcagcgctgg
      781 ctctgggggg gaaagccttc cgccagccgc acatctcgac atcatcgaca gctcttggga
      841 gtacgaggca tggcacgccc gatgacgggc gccgaaatgg tcatcacggc gctacaggac
      901 cagggagttg agttcatctt tggctatccg ggtggcgccg tactcccgat ctacgacgag
      961 atcttccagc aggaaaacgt caagcacgtg ctcgtgcggc aggagggtgg ggcggttcat
     1021 gccgccgagg gctacgcccg ctcgacaggc cgcgtcggcg tggtgctggt cacctccggt
     1081 cccggcgcca cgaacgccgt cacgggcctg accgatgcgc taatggattc gatcccggtc
     1141 gtctgcatta cggggcaggt gccgacccac ctcatcggct ccgacgcctt tcaggagtgc
     1201 gatacggtcg gcatcacccg gtcctgcacc aagcacaact ggctggtgaa agacgtcaac
     1261 gaccttgccc gtattctgca cgaggccttc tatgtcgcca ggaccggccg tcccggtccc
     1321 gtggtggtcg acattccgaa ggacgttcag ttcgcgaccg gcaactatgt cggcccgtcg
     1381 aatatcgccc ataagaccta caagccccgg gtgaaaccgg agaccgcggg cgtgcgggaa
     1441 gcgatcgagc tgatggcgac ggccaagaag ccggtcttct acacgggcgg cggcgtcatc
     1501 aactccggcc cgaaggccag ccagctcctg cgcgagctgg tccggctgac ggggttcccg
     1561 atcacctcga cgctgatggg gctcggcgcc tatccggcat ccgacaagca gtggctgggc
     1621 atgctcggca tgcacggcac ctacgagtcc aacatggcga tgcacgattg cgacgtgatg
     1681 atcgccgtcg gcgcgcgttt tgacgaccgg attaccggcc gcatcgatgc cttctctccg
     1741 agctcgaaga ttatccacat cgatatcgat ccgtcctcga tcaacaagaa cgtgcacgcg
     1801 cacgtgccga tcatcggcga cgtcgcgtac gtcctggagg acatggtcgg gctttggaaa
     1861 cagcgcgccc cgcaggtcga caagacggcg cacaaggcct ggtggaagca gatcgaccag
     1921 tggcggacgc gcaaatcgct gagctacaag gcctccgccg atgtgatcat gccgcagtat
     1981 gccatcgagc ggctctacgc gctgaccaag gaccgcgacg tctacatcac caccgacgtc
     2041 gggcagcacc agatgtgggc ggcccagttc ttccggttcg aggagccgaa ccgcatgatg
     2101 acgagcggcg gtctcgggac gatgggctac ggcctgccgg ccgccatcgg cgcccagatc
     2161 gcgcatccga aatcgctcgt cgtcagcgtc tcgggtgatg cctcgatcct gatgaacatc
     2221 caggagatgt cgacggccgt tcagtaccgc ttgccggtca aggcattcat cctcaacaac
     2281 cagtacatgg gcatggtccg gcagtggcag cagcttctgc acggcaaccg gatctccgag
     2341 agctacaccg aggccctgcc ggacttcgtc aagctggccg aggcctatgg cggcatcggc
     2401 atccgctgcg acaggcccgc ggatctcgac gatgcgatcg aggagatgat ccgcacgccc
     2461 aagcccgtgc tgttcgactg ccgcgtcgcc gccctcgcca actgcttccc gatgatcccg
     2521 tcaggcaagg cgcacaacga gatgctgctc ggcgaggagg tgtccgacga ggagatcggc
     2581 agcgtgatcg gagagagggg caaggtgctg gtgtaggtgc gagccaccga atccggttga
     2641 ccgtggcccg gacctcttct acgtcttact ctcgtcttac caacgtgagg agtggcgaga
     2701 tggaaacgac tcgcctgtcg agcaagggcc aggtgatcat accgaaggca gtgcgtgagg
     2761 cgcgcggctg ggagcccggg accgagctcg tcgtggagga gcatgccgac ggcgtgttgc
     2821 ttcgatcccg tcctgcattt gccgcgacac gtatcgatga tgtcgccggc tgcctgaagc
     2881 gccacggccc gccgaagtcc gtacgggaga tggacgaggc ggttgcggcg gaagcgcgcc
     2941 gaaggtggag gaagttcgag gcgcaatgat cgccatcgac acgaacgttc tcgtccgatt
     3001 cctcgttggc gacgatcccg agcagctcga acgtgcggca gccttggtga ggcgaaacga
     3061 gatcttcgtt gggctgaccg tgctcctcga gacggaatgg gtgctccgca gcgcttacgg
     3121 tgctacacgc gatgagatag tcgtcgcgtt ccggggcgta gccggactcc ggaatgtgaa
     3181 tgtggaggcg ccagccattc tcatggacgc catcaatgcg ctcgagcagg gcatggattt
     3241 cgccgacgcc ttgcaccttg ccggcgccgc tcacgcgagc gcggctgcgt tcgcaacgtt
     3301 cgacgaggat ctccgcaagc gggcgggtgc gctcggtcgg caggttcggg ttacagggct
     3361 ctgaggtctc gtcccatgca cctcaccatc gccaacaagc tctactcctc ctggtccctc
     3421 cgcccgtggc tggtgatgac ggcgttcggc attccgttcg aggagacggt gatcccgatg
     3481 taccagcccg attcgaaggc acgcatgcgc gcggcctcgc cgacgggcaa gatgccgtgc
     3541 cttgtcgatg gggacgtgac ggtctgggag agcctcgcga tcatcgagca tatcgccgag
     3601 cggttccccg agaagccgat ctggccgacg g
//
LOCUS       DASSGX010000060         3159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_10900, whole genome shotgun sequence.
ACCESSION   DASSGX010000060 DASSGX010000000
VERSION     DASSGX010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10900"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..1212)
                     /locus_tag="VFI12_00990"
     CDS             complement(25..1212)
                     /locus_tag="VFI12_00990"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7055000.1"
                     /translation="MLSPRSQRSIEQIAAGLAKIAIESSVTGVYAEAVSLLGRLPAVA
                     TVAVVRPDDQGLLTVTFQHGEPIEPALSASCARALHQQWIASHARDGDVTGPAPFLLP
                     SRDDVMLVPMVSGDVLLGGFILVGRSGIPPAVGSAERVAAAAVGTVTAQAIDNLLTRQ
                     RAALAVSEAERDAAQLQLRRSVGRELHDGPTQELALAGITLDRLIRTLGEEQAISADA
                     RQARDLIDKAVAGMRTVVGKLRVQDKPAASATGPLRALIAEMAPTTPSMELNINEVSG
                     VRLAPHVERAMIGIVREALHNVRKHARADEVRLEVRRIDDHVEIAVVDDGVGFAGGSP
                     DGHFGLEQIRELAEETGGQIEIGSAPGTGTSVRARIPMDTAGIRPDGQDSGSPGSPVP
                     RQK"
     gene            1494..2279
                     /locus_tag="VFI12_00995"
     CDS             1494..2279
                     /locus_tag="VFI12_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7055001.1"
                     /translation="MTQITFLGTGTSSGIPLIGCTCDVCRSADPRDKRTRTSAVIRHE
                     GRVFLVDTATELRIQALAAGLDHIDAVLFTHAHADHTGGFDDLRRFNELAQRHLPIYA
                     GPATAEILRERFAYAFTDVFPFYGGKPDLTLHVVDEPFTFDGIEITPLPVWHGQMRVF
                     GYRIGDVAYVTDAKDVPAETRELMTGLDILVLNALRERPHPTHLSVAQAVEIICELKP
                     RRAYLVHLSHELGHEAASRLLPSNVEVAYDGLTVSTDESSRPG"
     gene            2345..2728
                     /locus_tag="VFI12_01000"
     CDS             2345..2728
                     /locus_tag="VFI12_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055002.1"
                     /translation="MAGFIGFVVGLVATIAFLVAGAPLASRRIAANSAYGLRSADTDG
                     DDQIWHLANAAIGRDLIMTAGVNLLLTIVALVYWGDDSVQSAMTVSILLISIAGAIIA
                     VGRGLTTARSLGKAKTAFPPGMRRF"
     gene            2828..>3159
                     /locus_tag="VFI12_01005"
     CDS             2828..>3159
                     /locus_tag="VFI12_01005"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET7055003.1"
                     /translation="MKIAQVTPYDFAHPGGVAEHIQHLKTELNRAGHQVTIIAPRSRK
                     GGLEVGEDFYGVGRTVAIPGNKSRVRLTFDITLYSAVKELLHREAFDVIHLHEPLTPV
                     LPYMVLLNS"
BASE COUNT          515 a         1071 c         1039 g          534 t
ORIGIN      
        1 atctgaaatg acccttcggt cacctcattt ctggcgcggt accgggctgc cgggtgaacc
       61 agagtcctgc ccatccgggc gaatgccggc cgtgtccatc gggatgcggg cgcggaccga
      121 cgtccctgtg ccgggcgcgg atccgatctc gatctggccg ccggtctcct cggccagctc
      181 ccggatttgc tcgaggccaa aatggccgtc cgggctgccg cccgcgaacc cgaccccgtc
      241 gtcgacgacc gcgatctcga cgtggtcgtc gattcgccgc acttcgaggc ggacctcgtc
      301 ggcccgggca tgcttgcgga cgttgtggag cgcctcgcgg acgatgccga tcatggcgcg
      361 ctcgacgtgc ggcgccaggc gaacgccgga cacctcgttg atgttgagct ccatgcttgg
      421 tgtggtcggc gccatttcgg caatcagggc ccggagcggc ccggtggcgc tggcggccgg
      481 cttgtcctgg acgcgcagct tgccgaccac ggtgcgcatg cctgccacgg ccttgtcgat
      541 caggtctcgc gcctggcgag cgtcggcgga aatcgcctgt tcctcgccga gggtccggat
      601 caggcgatcg agcgtgatcc cggccagggc gagctcctgc gtcggcccat cgtgcagctc
      661 ccggccaacc gagcgacgaa gctggagttg agctgcgtcc cgctcggctt cggaaacggc
      721 gagtgccgcc cgttgccggg tcaggaggtt gtcaatcgcc tgggcggtga cggtgccgac
      781 cgcggccgcg gccactcgct cggccgatcc gaccgccgga ggaatcccgc tccggccgac
      841 caggatgaac ccgccgagca ggacatcgcc gctgaccatc ggcacgagca tgacgtcgtc
      901 ccggctcggc aacaggaagg gcgcggggcc ggtcacatcg ccatcgcgcg cgtgggaggc
      961 aatccactgc tgatgcaggg ctctcgcgca agaggcagac aaggcaggtt caatgggctc
     1021 gccatgctgg aatgtgacgg tcagcagccc ctggtcatcc ggccggacga ccgcgacggt
     1081 cgcgacggcc gggagccgcc caagcaggga tacggcttcg gcgtaaacac ccgtgacaga
     1141 ggactcgatc gcgatcttgg ccaagccggc tgcgatctgc tcaatgctgc gctgcgaccg
     1201 cggcgaaagc accgtcggcg cctggggaag ccggccggcc gggggaagcg gctcagattg
     1261 gcgtagcgac ccctgtgcaa tcatcggacg accttcactc ccgcgcggtt ggcgccgcgc
     1321 ccacaagccc tgcggtcatg gtagggatcg ggacatccgg cgagaaggcg cgggatatgc
     1381 cttttggtcc caaaggagga taggccggtc cgcaacggta cgccggaacc ggtggcgccg
     1441 ggaggtccgt ctatactgcg ttgcgacgtt cgggactgca tttgaaggat tccatgaccc
     1501 agatcacctt cctcggcacc ggcacttcga gtggcatccc cctgatcggt tgcacctgcg
     1561 atgtctgtcg ctccgccgat ccgcgagaca agcggacgcg cacgtcagcc gtcatccggc
     1621 atgagggtcg ggtctttctg gtcgataccg cgaccgaatt gcgcatccag gcgctcgccg
     1681 ccgggctgga ccacatcgat gctgtgctct tcacgcacgc ccatgccgac cacaccggtg
     1741 ggttcgacga tctgcgccgg ttcaacgagc tggcccagag gcatctccct atctatgccg
     1801 gaccggcaac ggcggaaatc ctccgggaac ggttcgccta tgcgttcacg gacgtcttcc
     1861 cgttctacgg cggcaaaccg gacctgacac tgcacgtggt cgacgagccg ttcaccttcg
     1921 acgggatcga gatcacgccg ctcccggtct ggcacgggca gatgcgggta ttcggctacc
     1981 ggatcggcga cgttgcctac gtgaccgacg ccaaggatgt cccggccgaa acacgcgagc
     2041 tgatgaccgg gctcgatatc ctcgtgctga acgcgctccg ggaacggccg cacccgacgc
     2101 acctcagtgt tgcccaggcg gtcgaaatta tctgcgagct caagccccgg cgtgcctacc
     2161 tggttcacct cagccacgag ctgggccacg aagcagcctc ccgcctgctt ccgtcaaacg
     2221 tcgaggttgc ctatgacggc ctgacggtga gcaccgacga gtccagccgg ccgggctgat
     2281 gatgaggaaa gtcccttcgg cgtggtagcc tcaccggagt ggaacagccg aaggggactg
     2341 gggaatggct gggttcattg gatttgtcgt cgggctggtc gcgacgattg catttctcgt
     2401 ggccggtgcg ccgctggcat cccggcgcat cgccgccaac tctgcctatg gcctgcggtc
     2461 ggccgatacc gacggggacg accagatctg gcacctcgcc aacgccgcga tcgggcggga
     2521 tttgatcatg acggccggcg tcaatctcct gctcacgatc gtcgcgctcg tctactgggg
     2581 cgacgactcg gtccagagcg cgatgaccgt ctcgatcctg ctgatctcga ttgccggcgc
     2641 gatcattgcc gtcgggcggg gcctgaccac ggcgcgatcg ctcggcaagg cgaaaacggc
     2701 cttcccgccc gggatgcgca ggttctgatc aggtgaccag aagcatccgc tatcctatgc
     2761 gcgtgaagca ccgtgccgat ccgccgcatc cgcgttccac ttcgtccatg ccccggagtc
     2821 cacgcacgtg aagatcgcgc aagtcactcc ctacgacttc gcgcaccccg ggggcgtggc
     2881 cgagcacatc cagcacctca agaccgagtt gaaccgggct gggcaccagg tcacgatcat
     2941 cgccccgcgc tcccgcaagg gcgggctgga ggtcggcgag gatttctacg gcgtgggccg
     3001 gaccgtcgcg atccccggca acaagtcccg cgtgcggcta accttcgaca ttaccctcta
     3061 cagcgccgtc aaggagctgc tgcatcgaga ggcgttcgac gtcatccatt tgcacgagcc
     3121 gctgacgccg gtactgccct acatggtgct gctcaactc
//
LOCUS       DASSGX010000061         2609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1207739, whole genome shotgun sequence.
ACCESSION   DASSGX010000061 DASSGX010000000
VERSION     DASSGX010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207739"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="VFI12_01010"
     CDS             complement(<1..580)
                     /locus_tag="VFI12_01010"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7055004.1"
                     /translation="MVAAARPATLPLPRTPLIGRESILAEIRALLLRPDIPLVTLTGP
                     GGVGKTRLALAVAESLLDDVDAVHFIRLATLRDPALVLPAIAQVLDVREVADRPLSEL
                     IQAAIDDRTVLLVLDNFEQVVDTANDLAPLLANCRNLTLLVTSRIVLHLSGEQAFPVP
                     PLTMITPAERATADDVTRSEAGTLFVTRATAAN"
     gene            complement(699..1556)
                     /locus_tag="VFI12_01015"
     CDS             complement(699..1556)
                     /locus_tag="VFI12_01015"
                     /inference="COORDINATES: protein motif:HMM:NF012995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HET7055005.1"
                     /translation="MTEGLRMTDPAPQQWVDRYLTLLGELAAAPSLPALSALVRAQFR
                     AVTFENVTSLLRRAAHPDGPVPPLDPDLLLANWEAGRGGGVCYEISAMFHRLIHALGY
                     DAELVLAQISIPDGHQALQVQLNGSRYLVDVGTGSPIFRPIPLIGTTEFRHAGLTYRF
                     RADDEPGHWRQERLIDGDWKLSCRYDLGPLDLDRQYIAYQHHQTPGATWVLDKIRLIR
                     CEDDAVYSLTGPELTTITTAGKRTDHLSDFTEYTQLAAETFGLPNLPINKAVDARPDF
                     IPIELRVKS"
     gene            1780..>2609
                     /locus_tag="VFI12_01020"
     CDS             1780..>2609
                     /locus_tag="VFI12_01020"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7055006.1"
                     /translation="MMTRGKRGATLIVAALLLSTMLAGFASNSAAQADRPTLRFAVNA
                     ADLATLDPHYASGTQDRTVVDMIFNGLVRYTPGNSATMEPDLATAIPEPEMKDGKQVW
                     TFTLRDGVMCHPSAQSEAYALTADDVVASLQKSANPDTSAFAGDYKGMTVAKVDDKTV
                     AITMDTPISPTLFLPKVANYAGGFIVCMQAYDALGADAFKTAPVGTGPFMFKSYTPQN
                     SVELVANDQYFRGAPKLAGVSVRYIADSSAREFGLAGGDLDLIAGLPEAQWVDKINAD
                     G"
BASE COUNT          435 a          753 c          965 g          456 t
ORIGIN      
        1 ggttggcggc agtggcgcgg gtgacgaaga gcgtgccggc ctcggagcgg gtgacatcat
       61 cggcagtcgc gcgctcggcc ggggtgatca tggtcagcgg cgggaccggg aaggcctgct
      121 cgccggagag gtggagcacg atccggctcg tgaccagcaa tgtgaggttg cggcagttgg
      181 cgaggagcgg ggcgaggtca ttggcggtgt cgaccacctg ctcgaagtta tcgagcacga
      241 gcagcacggt gcggtcgtcg atcgcggcct ggatcagctc ggacagcggg cggtcggcga
      301 cttcgcgaac atcgaggacc tgggcgatgg ccggcaggac aagggccggg tcgcgcaggg
      361 tggcgaggcg gatgaaatgg accgcatcga cgtcatcgag cagggattcc gcgacggcga
      421 gggcgagacg ggtcttgcca acgccaccgg ggccggtcag ggtgacgagc gggatgtcgg
      481 gccggagcag gagcgcgcga atctcggcca ggatcgattc ccggccgatc aggggggtgc
      541 gcgggagtgg caatgttgcg ggacgcgcgg cggcaaccat gacagggctc cgtcgagagg
      601 cggacgtgca ttgctgatga tagccgggcg aggttggttt ttggtctttg gtctctcgtc
      661 tttcgttttc cgtgttcggt tttccgttcc ctcaaccctt aactcttaac cctcaactcg
      721 atgggaatga agtcgggacg ggcgtctaca gccttgttga tgggcaaatt ggggaggccg
      781 aaggtttcgg cggcgagttg ggtgtattcg gtgaagtcgc tgaggtggtc ggtgcgcttg
      841 ccggcggtgg tgatggtggt cagctcgggg ccggtcaggg aatagaccgc gtcgtcctcg
      901 cagcggatga gccggatctt gtcgagcacc caggtagcgc ccggggtctg gtgatgctgg
      961 taggcgatgt actggcggtc aaggtcgagc ggaccgaggt cgtaccggca ggagagtttc
     1021 cagtcgccat cgatcaaccg ctcctgccgc cagtggccgg gttcgtcgtc ggcgcggaag
     1081 cgatacgtga ggccggcatg gcggaattcg gtcgtgccga tcagcgggat gggccggaag
     1141 atgggcgagc cggtgccgac atcgacgaga tagcgcgatc cgttgagctg gacctggagc
     1201 gcctggtggc cgtccgggat gctgatctgg gcgaggacga gctcggcgtc atagccgagg
     1261 gcgtggatca ggcggtggaa catggccgag atctcgtagc agactccgcc cccgcggccg
     1321 gcctcccagt tggcgagcag cagatcggga tcgagcggcg ggaccggccc gtccgggtgg
     1381 gcggcgcgac gcaggagcga ggtgacattc tcgaaggtga cagcgcggaa ttgcgcccgg
     1441 accagtgcgg aaagggcggg aagcgacggc gcggccgcca gttcgccgag cagcgtgagg
     1501 tagcgatcga cccactgctg cggcgcggga tcggtcatgc ggagtccttc agtcattcgt
     1561 gtcaccggtt cgtgtatcga gtcgtcgcga gcgcgtcgcc gaactatacc cgggcgcggt
     1621 ggcgacgccg gggtttgtgc catctcgtca ggagcggcta ctatggcact tgcccctggc
     1681 actcacggat gcatctcacg agcaggcatc cattttttcg acggcgtcgt cggaagggga
     1741 ggttgaccat cccggggctt gtaccgcgca aggaggtcga tgatgactcg cggcaaacga
     1801 ggcgccacac tgatcgtggc ggcgctcctt ctctcgacga tgctggccgg gtttgccagc
     1861 aatagcgccg cccaggccga tcggccgacg ctgcggtttg ccgtcaatgc ggcagatctg
     1921 gcgacgctcg atccccacta cgcctccggc acccaggacc ggaccgtcgt cgacatgatc
     1981 ttcaacggcc ttgtccgcta tacgccgggc aactccgcca ccatggagcc ggatctggcg
     2041 acggcgatcc ccgagccgga gatgaaggac ggcaaacagg tctggacctt cacactgcgg
     2101 gacggtgtga tgtgccaccc gagcgcgcag tccgaggcgt atgcgctgac ggccgatgac
     2161 gtcgtcgcct ccctccagaa gtcggccaac ccggatacct ccgcttttgc cggtgactac
     2221 aagggcatga cggtcgcgaa ggtcgatgac aagaccgttg cgatcacgat ggacacgccg
     2281 atttcgccga cgctcttcct gccgaaggtg gccaactatg ccggtggctt catcgtctgc
     2341 atgcaggcgt atgacgcgct cggcgccgat gcgttcaaga cggctccggt cggcacgggc
     2401 ccgttcatgt tcaagagcta taccccgcag aacagtgtcg agctggttgc caatgaccag
     2461 tacttccgcg gagcgccgaa gctggccggt gtcagcgtcc gctacatcgc ggattcctcg
     2521 gcccgcgagt tcggcctggc cggtggcgac ctcgatctga tcgcgggtct gcccgaggcg
     2581 cagtgggtgg acaagatcaa tgccgacgg
//
LOCUS       DASSGX010000062         2896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_11428, whole genome shotgun sequence.
ACCESSION   DASSGX010000062 DASSGX010000000
VERSION     DASSGX010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11428"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1124
                     /gene="gndA"
                     /locus_tag="VFI12_01025"
     CDS             <1..1124
                     /gene="gndA"
                     /locus_tag="VFI12_01025"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF006765.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HET7055007.1"
                     /translation="DTIRRGKELDGTGLRYMGMGVSGGEEGARYGPSLMPGGPKEAYD
                     LVEPVLTKIAAQVKDGPCVSYIGPDGAGHYVKMVHNGIEYGDMQLIAETYDIMKRALG
                     MSDAEMAEVFGRWNEGKLSSFLIEITAKVLSTIDPITGQPLVEKILDAAEQKGTGRWT
                     SQNALDLGTPIPVIDAAVFARSISSFKAKRVGASKLLTGPSVTVEVSGDDKKRLIGVL
                     EDALYVAKISSYAQGMALLRAASDAYEYGLNLAELARIWTGGCIIRAKLLEPIMTAFR
                     NNPALDNLLIAPEFVDTVNSATNACREAVILARKHGLPCPAMSAALDYIDSYRTERLP
                     ANLIQAQRDFFGAHTYKRIDTAGVFHADWDTGKAEEIKG"
     gene            1188..2813
                     /gene="zwf"
                     /locus_tag="VFI12_01030"
     CDS             1188..2813
                     /gene="zwf"
                     /locus_tag="VFI12_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HET7055008.1"
                     /translation="MSDSRVITASGGTLTAERARHEPASKTYNPLRLGLQSERNPQAC
                     AMVIFGVSGDLTSRKLMPALYDLYVSVPLPPGFSIVGISRRDWSDDAFRAEMRDAVKE
                     HATRPFTDEAWDSFARGLFYVQGDFDDSGLYDQLRDRLETVDTERGTNGNHLYYLATP
                     PSFYAEIAAGLGNAGLAKRQEIYSNPRAQGWNRIVIEKPFGHDLKSAQHLNAAINAVF
                     AEPQIYRIDHYLGKETVQNVLAFRFANILFEPVWNRHYVDQIQITVAESLGIENRAAY
                     YEEAGALRDMVQSHMLQLLSVVAMEPPAFFNGDALRDEKVKVLRSVVPPDGDRVAMDV
                     VRGQYTDGYVAGTEVVGYRDEEKVNPDSRTETFVALKLEIDNWRWNGVPFYLRTGKRM
                     PRRVTEIAIQFKSVPHLMFKLTEGQTLPPNVLTMRIQPDEGISLRVAAKVPGAGTKLQ
                     PVQMDFDYGASFGQAGPDAYVRLLLDAMLGDPTLFARDDEIDTAWSLMQPILDVWADQ
                     PVTMQIPSYEAGTWGPVEAELLLANDRRTWRRP"
BASE COUNT          552 a          970 c          908 g          466 t
ORIGIN      
        1 aggacaccat ccgccgcggc aaggagctcg acggcaccgg cctccgctac atgggcatgg
       61 gcgtcagcgg cggcgaggag ggcgcgcgct acgggccgag cctgatgccg ggcgggccga
      121 aggaggcgta cgacctggtc gaaccggtgc tgaccaagat cgccgcgcag gtcaaggacg
      181 gcccttgcgt gagctacatc gggccggacg gcgcgggcca ctacgtcaag atggtccaca
      241 acgggatcga gtacggcgac atgcagttga tcgctgagac ctacgacatc atgaagcggg
      301 cgctcggcat gagcgacgcc gagatggccg aggtcttcgg gcgctggaac gagggcaagc
      361 tctcctcctt cctgatcgag atcaccgcga aggtcctctc gacgatcgac ccgatcaccg
      421 gccagccgct ggtcgagaag atcctcgacg cggccgagca gaagggcacc ggccgctgga
      481 ccagccagaa cgcgctcgac ctcggcacgc cgatcccggt cattgacgcg gccgttttcg
      541 cccgctcgat ctcgtcgttc aaggcgaagc gggtcggcgc gagcaagctc ctgaccggtc
      601 ccagcgtcac ggtcgaggtc agcggcgacg acaagaagcg gctgatcggc gtgcttgaag
      661 acgccctcta cgtcgccaag atctcgtcct acgcgcaggg catggcgctg ctgcgggcgg
      721 cgtccgatgc ctatgagtac gggctaaacc tggccgagct ggcccggatc tggaccggcg
      781 gctgcatcat ccgggcgaag ctgctcgaac cgatcatgac cgcttttcgg aacaacccgg
      841 cgctcgacaa cctgctcatc gcgcccgagt tcgtggatac tgtcaactct gcgacgaatg
      901 cctgccgcga agcagtcatt ctggctcgga agcacggcct gccgtgtccg gccatgagcg
      961 ctgcgttgga ttacatcgac agttaccgca ccgagcggct cccggccaac ctgatccagg
     1021 cgcaacggga cttcttcggg gcgcacacgt acaagcggat cgatactgcc ggcgtcttcc
     1081 atgccgattg ggacacgggc aaggcggagg agatcaaggg gtagcaaccg tttatgggcg
     1141 caacgaccac ccggcgccga cgtcgagtgc aggaaatgga gtctcgtatg tccgactccc
     1201 gcgtcatcac agccagcggc ggcaccctca ccgccgagcg cgcccggcac gagccggcga
     1261 gcaagacata taacccgctc cggctcggcc tgcaatcgga acgcaatccg caggcctgcg
     1321 cgatggtcat cttcggcgtc tccggcgatt tgacctcgcg caagctgatg cctgccctgt
     1381 acgacctcta cgtctccgtg ccgctcccgc ccggcttctc gatcgtcggc atttcccggc
     1441 gcgactggag cgacgacgcc ttccgcgccg agatgcggga cgccgtgaag gagcacgcga
     1501 cgcgaccctt caccgacgag gcgtgggact ccttcgcgcg cggtcttttc tatgtgcagg
     1561 gtgatttcga cgattcgggc ctctacgacc agcttcgaga ccgtctcgaa acggttgaca
     1621 ccgagcgtgg gacgaacggc aaccacctgt attacctggc tacgccgccc agcttctatg
     1681 ccgagattgc agccggcctc ggcaatgccg ggctcgccaa gcggcaggag atttacagca
     1741 acccgcgcgc ccagggctgg aaccggatcg tcatagagaa accattcggt cacgatctga
     1801 agagcgcgca gcatttaaac gcggccatca acgccgtctt cgccgagccg cagatttacc
     1861 ggatcgatca ctaccttggc aaggaaaccg tccagaacgt cctcgctttc cgcttcgcca
     1921 atatcctgtt cgagccagtc tggaatcggc attatgtcga tcagattcag attacggtag
     1981 ccgaatcgct cggcatcgaa aaccgggccg cctactacga ggaggcgggc gcgctgcggg
     2041 acatggtcca gagccacatg ctccagcttc tctcggttgt cgcaatggag ccgcccgcct
     2101 tcttcaacgg cgacgcgctc cgcgacgaaa aggtcaaggt attgcgctcg gtcgtgccgc
     2161 ccgacggcga tcgcgtcgcc atggacgtgg tccgcggcca gtacaccgac ggctacgtgg
     2221 ccggcaccga agttgtcggg tatcgcgacg aagaaaaggt caacccggat tcgcggaccg
     2281 aaaccttcgt cgcgctcaag ctcgaaatcg acaactggcg ctggaacggc gtgcccttct
     2341 acctgcgcac cggcaaacgc atgccccggc gcgtcacgga gatcgcgatc cagttcaaga
     2401 gcgtgccgca cctgatgttc aagctgaccg agggccagac gctcccgccc aacgtgctga
     2461 cgatgcggat ccagcccgat gagggaattt cgctccgggt cgcggccaag gtgccgggcg
     2521 ccggaaccaa gctccaacca gtccagatgg acttcgacta cggcgcctcg tttggccagg
     2581 ccgggccgga cgcctatgtc cgcctcctgc tcgacgcgat gctcggcgac ccgacgctct
     2641 tcgcccggga cgacgagatc gacaccgcct ggtcgctcat gcaaccgatc ctcgacgtct
     2701 gggccgatca gcccgtgacg atgcagatcc cctcctatga ggcaggtacg tggggaccgg
     2761 tcgaggccga gctgctgctc gccaacgacc ggcggacctg gcggcgacca tgacggatct
     2821 cctgcaacca gcgccactga cgcgtaatga acactgggaa tcgaaccaga tcagcccgaa
     2881 cgtgatcggc cgcgtc
//
LOCUS       DASSGX010000063         2693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_664469, whole genome shotgun sequence.
ACCESSION   DASSGX010000063 DASSGX010000000
VERSION     DASSGX010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664469"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VFI12_01035"
     CDS             <1..355
                     /locus_tag="VFI12_01035"
                     /inference="COORDINATES: protein motif:HMM:NF012779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HET7055009.1"
                     /translation="QNCRHLHLARHLSEKLEPCGDVCDICTTGPKIEASRRVIPKRRD
                     DPYFQALSQWRLEKAREMVVPAFVIASDRLLDRLAMDRPRSRAALLNTPGIGTIKANQ
                     YGEDILAIIRSVGAD"
     gene            complement(426..1679)
                     /locus_tag="VFI12_01040"
     CDS             complement(426..1679)
                     /locus_tag="VFI12_01040"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET7055010.1"
                     /translation="MTGAIEKARSRATEFVRARLDDVITLTEELVAAPSENLPGDETR
                     PAEVVRQWLPRLGLPEATTLGALPHRPNLLVTIDSGKPGPKLGICGHLDTKPVGDAAA
                     EWKTDPFTAMVIGERMYGLGTTDMKGAVAAMLVGGAAFNAVKNEVGGSLTLILTADEE
                     YGSTYGAEYLARAKALDVDGIVLGEPSGVHEDWEALRIVSRGISCFKVRVFGTQIHSS
                     ISDQFPVVNAVEHMARLYCGFRERFRPRYPEHPHCAGGPTLNIGVKTVGGVGFGVNAG
                     VAEFWSDVRTTPGMDLTTFQHDVEWALDEAATEIPGVRYEVEYPPHLTWLEPTEVPAD
                     HPLTLACLDAGRRVLDREIPLAAFPGASDAYPFQFIGGIPTIASFGPGWLTPAHGPNE
                     WVSISSLTEAAEIYAQLAITYCAGA"
     gene            complement(1684..2613)
                     /locus_tag="VFI12_01045"
     CDS             complement(1684..2613)
                     /locus_tag="VFI12_01045"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HET7055011.1"
                     /translation="MDKSARFRSVDCLTIIEQRIPALTGASRQVAEAIMRDPWAVLGM
                     TIYDVAEMSNVSLPSVTRFCRAVGFGGFRELVQGIAQSLGRIDSKDFESLHQSDHHDG
                     GIKALAESIVNRQVEALQTTLHALDYEAIERATQMIAEARRVVILGHGAAQVPALGLS
                     VKLMWGGVAAFEAAPDMFSNIVITSQPGDVVIGVSHQGRTRDTIELLRLARKFGANTI
                     GITTVPNSPLAEVSDVSLAVLSPDVARAGTFLIAFDALMVVADILAATVSDRKWNGDP
                     PNRSDVVEWIERNLRVGPSTFVDTRHARRVPNG"
BASE COUNT          475 a          927 c          846 g          445 t
ORIGIN      
        1 ccaaaactgc cgccacctcc acctcgcccg gcacctgagc gagaagctcg agccgtgcgg
       61 cgatgtctgc gacatctgca cgaccgggcc gaagattgag gcgtcgcgcc gggtgattcc
      121 gaagcggcgg gatgatccct acttccaggc gctctcgcag tggcggctcg aaaaggcgcg
      181 cgaaatggtc gtgccggcct tcgtcatcgc gtcggaccgg ctgctcgacc ggctcgcgat
      241 ggaccggcca agatcccgcg ccgccctgct caacacgccc ggcatcggca cgatcaaagc
      301 caaccagtac ggtgaggaca tcctggccat catccgcagc gtcggtgcgg actgacggtg
      361 cccattcgaa cgtagcgcgg ggttaccatg ccccgctccg cgccccgtaa aggcggcgga
      421 atgtttcagg cgcctgcgca ataggtgatc gcgagctgtg cgtagatctc agctgcctcc
      481 gtaaggctgg agatcgagac ccactcgttc ggaccgtgcg ccggggtcaa ccagccaggc
      541 ccgaacgatg cgatcgtcgg gatgccgccg atgaactgga acgggtacgc atccgacgcg
      601 cccggaaagg cggcgagcgg gatctcccgg tccagcaccc ggcgtcccgc gtccaggcag
      661 gcgagcgtca gcgggtgatc cgccggcacc tctgtcggct ccagccaggt aaggtgcggc
      721 gggtattcga cttcgtagcg gacaccggga atttccgtcg cggcctcatc gagcgcccat
      781 tcgacgtcgt gctggaacgt agtcaggtcc atgcccggcg tcgtgcgcac gtcggaccag
      841 aactcggcca cgccggcgtt gaccccgaac ccgaccccgc cgaccgtctt gacgccgata
      901 ttcagcgtcg gtccgcccgc gcaatgcgga tgttcggggt accgcggccg gaagcgctcc
      961 cggaacccgc agtagagccg ggccatatgc tcgaccgcat tcacgaccgg gaattggtcc
     1021 gagatcgacg agtggatctg cgtgccgaaa acgcgcacct tgaagcagga gatgccgcgc
     1081 gatacgatcc ggagcgcctc ccaatcctcg tgcacgccgg acggttcgcc gagcacgatc
     1141 ccgtcgacat caagcgcctt cgcgcgcgcg agatactcgg cgccgtaggt gctgccatac
     1201 tcctcgtcgg ccgtcaaaat cagcgtcagc gagccgccga cctcattctt gaccgcgttg
     1261 aaggcagccc cgccgaccag cattgccgcg accgcgccct tcatatctgt cgtgccgaga
     1321 ccgtacatcc gctcgccgat gaccatggct gtgaatggat ctgttttcca ctccgcggcc
     1381 gcgtcgccga ccggcttcgt atcgaggtgg ccgcagatgc cgagcttcgg accgggtttg
     1441 ccagaatcga tcgtgaccag cagattcggc cggtgcggca aggcgccgag cgtggttgcc
     1501 tcgggcaatc cgaggcgcgg cagccattgc cggacgactt cggccggtcg agtttcgtcg
     1561 cccggcagat tctcgctcgg agcggcgacc agctcctcgg tcagggtgat cacgtcgtcc
     1621 agccgcgccc ggacgaactc cgtcgcgcgt gaccgggcct tctcgatcgc tccggtcatg
     1681 cccctatcca ttcggcacgc gccgggcgtg ccgcgtatcc acgaatgtcg acggtccgac
     1741 ccgcaagttg cgctcgatcc actccacgac atcgctgcgg ttcggtggat caccgttcca
     1801 tttccggtcg gaaacggtag ccgccagaat atcggccacc accataagcg catcaaaggc
     1861 gatcaggaag gttcccgccc gggccacatc cggcgagagg acagcgagcg acacgtcgct
     1921 cacctcggcc agcggcgagt tcgggacggt cgtgatgccg atcgtgttcg cgccaaactt
     1981 gcgggcaagg cgcagcagct cgatcgtgtc gcgcgtccgt ccctggtgcg agacgccgat
     2041 cacgacgtcg cccggttgcg aggtgatcac gatgttcgag aacatgtccg gcgccgcctc
     2101 gaaggccgcg accccacccc acatcagctt gaccgaaagc cccaacgctg ggacctgcgc
     2161 ggcaccgtgc ccgaggatca cgacccggcg ggcctcggcg atcatctgcg tcgcccgctc
     2221 gatcgcctca tagtcgagcg cgtgaagcgt ggtctgcaac gcctcgacct gacggttgac
     2281 gatcgactcg gcaagcgcct tgattccgcc gtcatggtgg tcgctctggt gcaacgactc
     2341 gaaatccttg gagtcgatcc gtccaagcga ctgcgcaatg ccctgcacca gctcccggaa
     2401 gcctccgaat ccgaccgccc ggcagaagcg ggtcacggag ggcaggctga cattgctcat
     2461 ctcggcgacg tcgtagatcg tcatgccgag cacggcccat gggtcgcgca tgatcgcctc
     2521 ggcgacctgg cgcgatgcac cggtcagcgc cggaattcgt tgctcgatga tcgtgaggca
     2581 atcgacagag cgaaatcggg ccgacttgtc catgcctgtc cctcacccga aaggcggtta
     2641 cgcggaaaga aagaaacttt cgcgtattct acggtccgct ttcagaccgt caa
//
LOCUS       DASSGX010000064         2372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_446648, whole genome shotgun sequence.
ACCESSION   DASSGX010000064 DASSGX010000000
VERSION     DASSGX010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446648"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      complement(164..247)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(737..1519)
                     /locus_tag="VFI12_01050"
     CDS             complement(737..1519)
                     /locus_tag="VFI12_01050"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HET7055012.1"
                     /translation="MTETTGRTTQPHEWYQDLLGAYALDALLPDESAALEAHLATCAE
                     CQAELRRLRFGANAYALVAEERSPVPELRDRLWAAISAGPNVASEPEPVAAPVAPPIS
                     IERAHAARRLPAPPLWSRIAAVLAVILIAGLLAWNISLRSEESGGNGTVVAQLAAAPD
                     AADQATGGEARYLTDKQVLLISLHDLPALPQGEVYQLWLIQGSTVLPSVTFQPGPEAT
                     TTVAVAANPDTFDTLAITREPGPIGSAAPTTKPFVTGVVRRD"
     gene            complement(1577..2182)
                     /locus_tag="VFI12_01055"
     CDS             complement(1577..2182)
                     /locus_tag="VFI12_01055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7055013.1"
                     /translation="MSTPTVGTDTDSALIGRVGGGDGAALMALYDRHNRAAFGLAYRI
                     LGDAATAEEVVQDAFLSLWRNAKSFDTSRGGVRTWLLTIVHNRAIDRIRAAKSRGSTV
                     ELEVADFAGIADDPWDQVTDRLDGAEVREAVADLPPDQRDAIELAYFQGLTHQEIAAR
                     MQLPLGTVKGRLRLGLRKLATALSPPEAIPAERDRAGPDDP"
BASE COUNT          429 a          807 c          785 g          351 t
ORIGIN      
        1 ggaaggagac cagatgcccg gggaaggacg gcgaccggaa tcatcccgaa ggcgaagacc
       61 aatcaaccgc cgaggcgccg atacgacgcc gttccatcgt gtgccgcgga acaacggaca
      121 ggccgcccgg accacgcacg cgcgcacgca aaaaccggct cctctcgaag gcggccggtt
      181 tcaccacttg ctccacatcg cccaggtgac caggtacagg cgatggatca gcgcatccgt
      241 tgtccggtgg aaagtgatcc cggtaccgaa gggtcatccc ccggccccag gaccaatctc
      301 ctaatcgcca cgatatggcc ggcggcgcgc aagagcatcc ggtcgggcta ggaagatcag
      361 gctgttaggg gtggtacctc ggtactgagg agagcagaca ggaggcgagc aggcgagcag
      421 ttggggcacg gcggcgcgga cacgaaatgt agcgtcgcat ggtaatgcga catcccgaaa
      481 tcgcagctca gcctcggaga ggctttctgg ctgagcccgg ggttgatgat cgttgtcggt
      541 ctatctcggt aacggcgccc tgtcccttag ggacatccgg accctggctc tggatttcaa
      601 tccggagcgg cgccgaaccg cccggtaccg attcaatccg gcaaccacca taacgcccgg
      661 gaatcacgga ggccaacacg gaaaacggaa aacggaaaac gaaagaccag agacgaaaga
      721 ccaaaaacca cacacctcag tcgcgccgca cgactcccgt cacgaagggt ttcgtcgtcg
      781 gcgcggcgga gccgatgggg ccgggctcgc gagtgatggc aagggtgtcg aaggtgtccg
      841 ggttggcggc aacggcaacc gtcgtcgtcg cctccgggcc cggttggaag gtgacggacg
      901 gcagcaccgt actgccctgg atcagccaga gctggtagac ctcaccctgc ggcagagccg
      961 gcagatcgtg cagcgagatc agcagcacct gcttgtccgt gaggtagcgc gcctcaccac
     1021 cggtcgcctg gtcggccgcg tccggcgcgg ccgcgagctg ggcaactacc gtgccattcc
     1081 cgccgctttc ctcgctccgc agcgagatgt tccaggccag cagcccggcg atgagaatga
     1141 ccgcgagcac cgccgcgatc cgggaccaga gcgggggtgc gggtaaccgc cgcgccgcgt
     1201 gggcccgctc gatcgagatc ggtggcgcga ccggagcggc gaccggctcc ggttcggacg
     1261 cgacattggg tccggccgag atcgccgccc acagccggtc ccgcagctcc ggcactgggg
     1321 accgctcctc ggcgaccagg gcgtaggcgt tcgcgccgaa ccgcaaccgc cgcagctccg
     1381 cctggcattc cgcgcaggtc gccaggtgcg cctcgagcgc cgccgactcg tccggcaaga
     1441 gcgcatcgag ggcataggcg cccaggagat cctggtacca ctcgtgcggt tgggttgtgc
     1501 gccctgtcgt ctcggtcatg ccaacgatcg tttcgtgcct gtcgatactc cgatgtgccc
     1561 gggttcctta catctattac ggatcatccg gccctgcccg atcacgctcg gccggaattg
     1621 cctccggcgg cgagagagcc gtcgccagct tgcgcaaccc cagccgcagc cggcccttga
     1681 ctgtccccag cgggagctgc atccgcgcgg cgatctcctg gtgcgtcagc ccctggaaat
     1741 aggccagctc gatcgcgtca cgctggtccg gcggcaggtc ggcgaccgcc tcccgcacct
     1801 cagcgccgtc gagccggtcc gtcacctgat cccacggatc atcggcgatg ccggcgaaat
     1861 cggcgacctc cagctcgacc gtgctgcccc ggctcttcgc ggcccggatc cggtcgatcg
     1921 cccggttgtg gacgatcgtc agtagccagg tgcggacgcc gccgcggctg gtatcgaacg
     1981 atttcgcgtt gcgccaaagg gagagaaagg catcctgcac gacctcctcg gccgtggcgg
     2041 cgtcgccgag gatacgatag gcgaggccga aggcggcgcg attgtggcgg tcgtagagcg
     2101 ccatcagggc agcgccgtct ccaccgccga cgcggccgat caacgcgcta tcggtgtccg
     2161 tcccgaccgt cggagtgctc acccgggcct tccccgcgct gccatatttc gctcgggcgc
     2221 aatcgtaccg cacctcgcgg cgcgatccgt agcgcgcatg aaggtgcccg gcggagcctc
     2281 tgcgatcatg cgttcgatcc cagtagcggg atgtggagga cgacagaaca tggcagcaga
     2341 agccccggca cacggatccg acatcacatc ac
//
LOCUS       DASSGX010000065         5389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_991260, whole genome shotgun sequence.
ACCESSION   DASSGX010000065 DASSGX010000000
VERSION     DASSGX010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5389
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_991260"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..980)
                     /locus_tag="VFI12_01060"
     CDS             complement(<1..980)
                     /locus_tag="VFI12_01060"
                     /inference="COORDINATES: protein motif:HMM:NF025009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HET7055014.1"
                     /translation="MSQRRRDTLPKKRNAAGGSASQRRISRREREERQRRQLYIGLAI
                     AGILSIVILAGFALNDYWFKPRAVLASVNGTEIRRRDYWKVRSVELVNQVNQYNQFSQ
                     LVDPSQQQQYLTLAQQAAAELDTVWGSTSINDATLTRMVEDQVYLQSLKDMGLSISDQ
                     DVQDYIWQQFEPQNAPIFTPTPAPTLIPQRAEWATQTETALKAETETPPAEVAGSPVA
                     GTPGSTQATPIGSPAAGTPVAEGSAGALATPVMNASPIETIATPIAGQGTPGTPAASP
                     AGSPVSPATPIGEASPTAEPATPNPTEAIQTAEANYGNYRDAVFGVAHLSR"
     gene            complement(1081..1512)
                     /locus_tag="VFI12_01065"
     CDS             complement(1081..1512)
                     /locus_tag="VFI12_01065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HET7055015.1"
                     /translation="MYAKAQVIGNLGRDPETRYTKSGAMNVSFSVAASRRFNDASGTL
                     QERTTWFRVTAWGKLAETLDRLAQDGALTKGQRVFVAGRLEADEYTGNDGQKRTSLEI
                     NADDVLLMSPRGEGSGQGGYGASRSSQQPAAVAEDVDDLPF"
     gene            complement(1545..2168)
                     /gene="rpsF"
                     /locus_tag="VFI12_01070"
     CDS             complement(1545..2168)
                     /gene="rpsF"
                     /locus_tag="VFI12_01070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HET7055016.1"
                     /translation="MSTEYKRIRDYELMVIFHPELSEEDLVSESGKVEEHITGVSGEI
                     RLINREAPWGRRRLAYPIRNGSRDIRDGVYVLYYFSAESGQIEEIEREIKLQDRIIRY
                     LLTQQTVPTMEPAPAEGEGEEGGEAEIAAAPEADSTATGAPVEGAPTETAATADTTSE
                     EPTPAPAEAGTESETPAEVDAESESPAEADAESETPAEADAESETPA"
     gene            complement(2301..3497)
                     /gene="cysS"
                     /locus_tag="VFI12_01075"
     CDS             complement(2301..3497)
                     /gene="cysS"
                     /locus_tag="VFI12_01075"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HET7055017.1"
                     /translation="MNLYNSATRSVEPLTVVDNHVGIYVCGVTPYDTTHAGHAFTFLT
                     FDILVRYLRSLGHDVTYVQNVTDIDDDILRKAKEVGLTWDELGRRETEKYLDDMRQLN
                     ALPFDHYLRATETVPLMVSLIEALVAEGHAYEANGSVYFSVATDPEFGKLSRIPRGEM
                     LAVANERGNNPDDPNKRDPLDFVLWQAAKPGEPTWNSPWGPGRPGWHIECSAMSMSVL
                     GDTVDIHGGGADLIFPHHECEIAQSENATGVKPFTRYWMHVAMVEYQGEKMSKSLGNL
                     VIASNVLQDFTADAFRLYLFSHHYRDAWEYVDAEIEPWADLARDMCEAVDTPSYSIGE
                     MLDSSVYRTRFTAAMDNDLDTPAAIEQLRELTSEILAAEDTDISPAQRELRQLAGILG
                     LTLSDA"
     gene            3605..4303
                     /locus_tag="VFI12_01080"
     CDS             3605..4303
                     /locus_tag="VFI12_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055018.1"
                     /translation="MPAQSSRLFHRPRRVNALIAGFTLTLLAACGGPDLATPTDNSPT
                     PTAIPTAARPTAAVGRTTGASCESGTLRVKDLPEIEKRWRDGLSMARGRAAKWKEDAV
                     LIELGISCELFEPGFRWQATFFSRDAQAYYRSDTTEVIPVNTDPDAIIPLPDSEINFA
                     RLHEVLMTSPDVDANGDDPITALDVRVSTQLQPIGPPGVPTGAAIYHVAVRTRGMVLE
                     LYIDAVQGQIYQFQ"
     gene            4313..5125
                     /gene="otsB"
                     /locus_tag="VFI12_01085"
     CDS             4313..5125
                     /gene="otsB"
                     /locus_tag="VFI12_01085"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HET7055019.1"
                     /translation="MRAADRLLELAEMGRMAIYADIDGTICPIAATPSAAILYPGAAA
                     ALDAIRRGGVRVVAISGRAAEDARRLVGLPDIDYAGNHGFELMTASGRVISEDVAAAS
                     LSVEAALGEVTALAAALPDGILIEDKAYTASIHYRLTKDPGASAVVLKAVLSEIAERH
                     GLLLTEGRLVFELRPRLDINKGVFVTNDVRLHGIKTAAFLGDDITDLDGFRALRDLTG
                     AGELLASVSIGVQAPESPARLLAESDILVDSVAGMVVELQRFADALAKWSRA"
     gene            5122..>5389
                     /locus_tag="VFI12_01090"
     CDS             5122..>5389
                     /locus_tag="VFI12_01090"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HET7055020.1"
                     /translation="MMRSAGLLIGLLLISLPVIAIAKPAVPSWRDTHPWSTEVATVQV
                     GDQELQAEIADTSALQTRGLGYRDGLAPGTGMIFVFDGASVHTFW"
BASE COUNT          918 a         1696 c         1720 g         1055 t
ORIGIN      
        1 cgcgataggt gcgcgacacc gaagaccgcg tctcgataat tgccgtaatt cgcctcggca
       61 gtctgtattg cttcggtcgg attcggcgta gccggttcag cagtcggcga ggcctcgcca
      121 atcggcgtcg ctggagagac cggtgaccct gccggcgagg cggctggagt gcccggcgtg
      181 ccctggcccg cgatcggggt tgcaatggtt tcgatcgggc tggcgttcat caccggggtc
      241 gccaacgcgc cggcgctgcc ctccgcgacg ggcgtgccgg cggcaggcga gccgatcggc
      301 gtcgcctggg ttgatccggg agtgccggca accggtgagc ccgcaacctc ggccggcggt
      361 gtctcggtct cggccttgag agccgtctcc gtttgcgttg cccattcggc tcgctgcggg
      421 atcagcgtcg gcgccggggt cggcgtaaag atcggcgcgt tctgcggctc gaactgttgc
      481 cagatgtagt cctggacgtc ctgatcggaa atcgacagac ccatatcttt caggctctgg
      541 agatagacct gatcctcgac catgcgggtc agggtggcgt cgttgatact ggttgagccc
      601 cagaccgtgt ccagctcggc ggcagcctgt tgcgcaagcg tcaggtattg ctgctgctgg
      661 ctcggatcga cgagctggga gaactggttg tactggttga cctggttgac gagctctacc
      721 gagcggacct tccagtaatc gcgccggcga atctcggtgc cgttgaccga tgcgaggacc
      781 gcgcgcggct tgaaccagta gtcgttaagc gcaaacccgg cgaggatcac gatcgagagg
      841 atgccagcaa tcgcgaggcc gatatagagt tgtcgtcgtt gtcgctcttc gcgctcgcgt
      901 ctggagatcc ggcgctggct ggcggagccg cccgcggcat ttcgtttctt cgggagcgta
      961 tcccgccggc gttgcgacat aaacctcaaa ctctccagac cacgacgagg gcaagagcgc
     1021 aaagcgccgc tgaatcacgg gcgaaccgat cgccgcgcca tgattcagcg gcaagtgagt
     1081 ctagaagggc agatcgtcca cgtcttcggc tacggccgcc ggctgctggg acgaccggct
     1141 cgcgccgtat ccgccctgac cgctcccctc accccgtgga gacatcagca aaacatcatc
     1201 ggcattaatt tcaagcgacg tgcgcttctg accgtcgttg ccggtatatt catctgcctc
     1261 gagccggcca gcgacgaata ctcgctgccc cttggtcaat gctccatcct gcgccagacg
     1321 gtcgagcgtt tcagccaact tgccccaggc ggttacccgg aaccaggtgg tgcgctcctg
     1381 caaggtgccg gaggcgtcat tgaatctgcg cgatgcggca accgagaacg acacgttcat
     1441 cgcgccagac ttggtgtatc gcgtttcggg atctctcccc agattaccga tgacttgtgc
     1501 cttcgcgtac atatcatctc cttcgatgaa gcagaccccg gttcttaggc gggcgtctca
     1561 gattcggcgt cggcctccgc aggcgtctca gattcggcgt cggcctccgc aggcgactca
     1621 gattcggcgt cgacctccgc gggcgtctca gattcagtgc cagcctccgc gggcgccgga
     1681 gtgggctctt cgctcgtggt gtccgccgtt gctgcagtct cggtcggtgc tccttcgacc
     1741 ggcgcaccgg ttgccgtgga atcagcctcc ggcgctgctg caatctctgc ctcgccaccc
     1801 tcttcgcctt cgccttcagc cggcgccggc tccatggtcg gtaccgtctg ttgcgtcagc
     1861 aaataacgga tgatccggtc ctgcagcttg atctcgcgct cgatctcttc gatttgcccg
     1921 gactccgccg agaaatagta gagcacgtag acaccatcgc ggatgtcgcg actgccattg
     1981 cgaatcggat aggcgagccg gcgccggccc catggcgctt cgcggttgat caggcggatc
     2041 tcgccgctga caccggtgat gtgctcttcg accttgccgc tctcgctgac caggtcttcc
     2101 tccgaaagct cggggtggaa gatcaccatc aactcgtagt cacggatgcg cttgtactcg
     2161 gtggacataa aaggacgagg ctccttcctc tggagtcgtc cccggccggc aatccggacg
     2221 acggggacag aaaggctcag gtattgaacc cacaaatcgg gcgagcctgc cgactcgccc
     2281 gactgcttat tctaccacga tcaggcgtcg gaaagcgtca aaccgaggat gcccgcgagt
     2341 tgccgtaact cgcgttgcgc cggggaaata tccgtgtctt cggccgccag aatctcgctc
     2401 gtgagctcgc gtagttgctc gatcgccgcc ggggtgtcga ggtcgttgtc catggccgca
     2461 gtgaagcgcg tccgatagac cgacgagtcg agcatctcac cgatcgaata cgacggggtg
     2521 tcgaccgcct cgcacatgtc ccgggcgaga tcggcccagg gctcaatttc agcgtccaca
     2581 tattcccagg catcgcgata gtgatgcgag aagaggtaca ggcggaacgc atcggctgtg
     2641 aagtcctgga gcacgttgct ggcgatcacg aggttgccga gcgacttgct catcttctcg
     2701 ccctggtact cgaccatcgc cacgtgcatc cagtagcgtg tgaacggctt gacgccggtc
     2761 gcgttctcgc tttgcgcgat ctcgcattca tgatgaggaa agatcaggtc ggcgccgcca
     2821 ccgtggatat cgaccgtgtc accgagaaca ctcatcgaca tcgcgctgca ctcgatgtgc
     2881 cagcccggcc ggcccggtcc ccagggtgaa ttccaggtcg gctcgcccgg tttggcggct
     2941 tgccagagga cgaaatcaag cggatctcgc ttgttcgggt catccgggtt gttgccacgc
     3001 tcgttcgcga cggccagcat ctcgccccgg ggaatccgcg atagcttgcc gaactccggg
     3061 tcggtggcaa ccgagaaata gacgctgcca ttcgcctcat aggcgtggcc ctcggcgacc
     3121 agcgcctcga tcagtgagac catgagcggg accgtctccg tcgcccggag atagtgatcg
     3181 aacggcagcg cgttgagctg ccgcatgtca tccaggtact tttcggtttc gcgccgcccc
     3241 agctcgtccc aggtcaagcc gacctctttc gccttgcgca agatgtcgtc gtcgatgtcg
     3301 gtgacgttct ggacgtatgt cacgtcgtga ccgagggagc gtaagtatcg aaccaggatg
     3361 tcgaaggtca gaaaggtgaa tgcatgaccg gcgtgggtgg tgtcgtacgg ggttactccg
     3421 cagacgtaga tgccgacgtg gttgtcgacg acggtcagcg gctcaaccga gcgcgtagct
     3481 gagttataga gattcatcgg gctcctgctt gcgcatcccg caaactgcgc ggtcgattct
     3541 cgattaccct gtgtgcacag gcatcacgtc agggcgtgag tataccgatc tcaagaggaa
     3601 tttcgtgccg gctcaatcca gtcgtctctt tcaccgcccg cgccgcgtca atgcgctgat
     3661 cgcggggttc acgctgacgt tgcttgccgc atgcggtggt cccgacctgg cgactccgac
     3721 cgacaattcg ccaacaccga ccgccatacc aaccgctgcc cgtccgactg ccgccgtggg
     3781 ccggacaacc ggcgcgtcat gcgaatcggg cacgttgcgc gtcaaggatt tgccggagat
     3841 tgagaagcga tggcgggatg gcctatcgat ggcgcggggc cgagccgcca aatggaagga
     3901 agatgccgtc ctgattgagc tcggtatctc gtgcgagctc ttcgagccgg gattccggtg
     3961 gcaggcgacc ttcttctcgc gggacgcgca ggcctattac cggtcggaca cgacggaagt
     4021 gatccccgtc aacaccgacc ctgacgcgat catcccgctg cccgacagcg agatcaattt
     4081 cgcgcgcttg catgaggtgc tgatgacgtc gccggacgtc gacgccaacg gcgacgaccc
     4141 gattaccgcg ctcgatgtcc gggtcagcac gcagctccaa ccgattggac cgcccggcgt
     4201 gccaaccggc gccgccatct atcatgtggc ggtgcgcacg cgtgggatgg tgctcgagct
     4261 gtatatcgat gcggtgcagg ggcagattta tcagttccag taggagtcag aggtgcgggc
     4321 tgctgaccga ttgcttgagc tagccgaaat gggccgtatg gccatttacg ccgacatcga
     4381 cggtacgatc tgtccgatcg cggccacccc gtcggcggca atcctctacc cgggcgcggc
     4441 ggcagctctg gacgcgattc gccggggtgg cgtccgggtc gtggcgatca gcgggcgggc
     4501 cgccgaggat gcccgaaggc tcgttgggct cccggacatc gattacgcgg gcaatcacgg
     4561 cttcgagctg atgaccgctt ccgggcgggt catctccgag gacgtggcag ccgcgtctct
     4621 gagcgtggag gccgcactgg gcgaggttac cgcccttgcc gccgccctgc cagatggcat
     4681 cctgatcgag gacaaggcat acaccgcctc gatccactac cggctgacga aggaccctgg
     4741 cgcctcggct gtcgtgctca aggcggtgct gtcggaaatc gcggagcggc atggattgct
     4801 cctgaccgag ggtcgacttg tcttcgagct gcgaccgcgg ctggacatta acaagggagt
     4861 cttcgtcacg aacgatgtgc gactgcacgg catcaaaacc gccgccttcc tcggggacga
     4921 catcaccgac ctcgacggat ttcgggcgct ccgggacctg actggagccg gcgaattgct
     4981 cgcaagcgta tcgatcggag ttcaggcgcc ggagagcccc gctcggctcc tggccgagtc
     5041 ggatatcctg gtggatagcg tggccggcat ggtcgttgag ctgcagcgct ttgccgatgc
     5101 actcgcgaaa tggagtcgcg catgatgcgc agcgctggcc tgctgatcgg actgttgctc
     5161 atctcgcttc ccgtgatcgc gatcgcgaaa ccggcagtcc cctcatggcg ggatacccac
     5221 ccctggtcaa ccgaagtggc gaccgtgcag gtcggcgacc aggagctgca ggcggagatc
     5281 gccgatacat ccgccttgca aacgcgcggc ctcgggtacc gcgatggtct cgcaccgggg
     5341 acgggcatga tcttcgtctt cgacggggca tccgttcata ccttctgga
//
LOCUS       DASSGX010000066         3756 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_882690, whole genome shotgun sequence.
ACCESSION   DASSGX010000066 DASSGX010000000
VERSION     DASSGX010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882690"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..302
                     /locus_tag="VFI12_01095"
     CDS             114..302
                     /locus_tag="VFI12_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055021.1"
                     /translation="MALSVLMVEDNPLNQELARDLLEAAGLAVEYHESDAAHYIDPPA
                     VLSARQWIDETLAQPATR"
     gene            complement(315..869)
                     /locus_tag="VFI12_01100"
     CDS             complement(315..869)
                     /locus_tag="VFI12_01100"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HET7055022.1"
                     /translation="MSERERMTWTIYDSPVGPLTLVAGPEGIRNIRFAGDAAPPAAAT
                     RGALPVLVEQLDSYFAGELRTFDLDLDLRGNPLQLLVWSQLLEIPYGATTTYGELAGR
                     INGDVYPEGLEPYKRARVVGAALGRNPVPVVVPCHRVIGADGSLVGFGGGLGRKRKLL
                     ELEGVVLEGRAGRSAADNLQLGLL"
     gene            939..2372
                     /locus_tag="VFI12_01105"
     CDS             939..2372
                     /locus_tag="VFI12_01105"
                     /inference="COORDINATES: protein motif:HMM:NF025761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HET7055023.1"
                     /translation="MLRAVFSIADRGYPARMILSAYRPTSSTYDEAFSPDGALRPGYE
                     ALLEALVEAGIRDTQDALRRRLSSMDVDFGDDESTFEVDPLPRLVSSAEWDRLSAGLA
                     QRHEAINAFIGDAYGDRSLIDHGVMPDRVLEESVYYEPMMEGLGVVAQLAGPDLIRDA
                     TGEMLVLEDNVRTPSGIAYAAASRSALRSAGQNFVEPSMLDPSLAYGLLHAVLRDANP
                     GGGDPSIVMLSDGPRSKAWFEHREICSRLNIPVVATGQLESRRKRLFALVGGEQLEVQ
                     VLYNRSSDESLRASDGELSRLGSLLKDPLEAGNVRCVNSFGAGIADDKAAHCYVEEMI
                     RFYLRERPLLRSVPSFDLGDPDQRATVMDRLDEFVIKPRWSFGGQGIVLGPRASELEL
                     QEIRARVERMPSGYVAQQMIEFSVHPTAVGDRLEPRHVDLRAFVATLNGKYTALPVAL
                     TRFAGGAGDLVVNSTQGGGAKDTWLLR"
     gene            2369..3154
                     /locus_tag="VFI12_01110"
     CDS             2369..3154
                     /locus_tag="VFI12_01110"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET7055024.1"
                     /translation="MRATGDTPLIGISASEIRPAQTIRQIQEGEPLSREVALGLEYVS
                     AVIGAGGLAVVLPPAGDCLAGALLDRIEGLCLSGGPDLDPSAYGASPHRCAGPADPTL
                     DRFEIDLAREAVQRDLPLLAVCRGMQVFNVSRGGTLIQHLPDCEWVTIDHRQEKAGNL
                     PSHGVHLDPASRISEVVGENTIAVNTFHHQGIASLGEDLEAVGWDDDGLVEAIEIPDR
                     RFALGVQWHAELMAGHEAGATLFSRLVESAREYRRNHPERVHS"
     gene            3151..>3756
                     /locus_tag="VFI12_01115"
     CDS             3151..>3756
                     /locus_tag="VFI12_01115"
                     /inference="COORDINATES: protein motif:HMM:NF016032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HET7055025.1"
                     /translation="MSDPAPPPPAWSEWKQRERAEYTIGLEEEVMLLSDDFSLANRVE
                     AVMPRLPELVAIQASRETQNSVIELCTEVHTDVGGAVEQLRSLRTELTDTLAALHLRG
                     ACAGTHPFAVWEEIAVTSGDRQELVHGSMRELARREPTFALHIHVGLADPELAIDVYN
                     SMRVHLPLLLALSANSPYWQGRDTGLASARTPLFQAFPRVGI"
BASE COUNT          627 a         1258 c         1238 g          633 t
ORIGIN      
        1 tgcagcgtct gcgcgtccgc gcggctcgac gcaggatctg cgcgaaacag ccggcgtgcg
       61 acaaaatgcc gcagtgcttc tcgcaggttg gcgcgttagg atcgaaggtg agaatggcgc
      121 tatcggtgct gatggtcgaa gacaacccgc tgaatcagga gctcgcccgc gacctgctcg
      181 aggccgccgg actggccgtc gagtaccacg agtccgatgc cgcccactac atcgatccgc
      241 ccgccgttct ctcggcccgg caatggatcg acgagaccct cgcccagccg gccactcgtt
      301 gaaggcgcga gcgctcagag cagaccgagc tgcaggttgt cggcggccga tcggccggca
      361 cggccttcga ggacgacccc ctccagctcc agcaacttgc gcttccgccc cagcccaccg
      421 ccgaacccga ccagcgaacc gtcggccccg atcacccggt gacatgggac cacgaccggg
      481 accgggttgc ggccgagcgc cgcgccgacg acccgcgccc gcttgtaggg ctcgaggccc
      541 tcgggataga catcgccatt gatccgcccg gccagctcgc cgtaggtggt cgtggcgccg
      601 taggggatct cgagcaactg actccagacg agcagctgca gcgggttgcc gcgcaggtcg
      661 aggtcgaggt cgaaggtccg cagctcgccc gcgaagtagg agtcgagctg ctcgacgagc
      721 accggcaacg cacccctggt ggccgcggca ggtggagccg cgtccccggc gaagcggatg
      781 ttcctgatcc cctccggccc ggcgaccagg gtcagcggcc cgacggggct gtcgtagatc
      841 gtccaggtca tccgctcgcg ctcgctcatc aggtccatgc atctaagacg ttggagcggg
      901 cgaaaacgtg agattggcag gctcagccgg agaccgaatt gctccgagct gttttctcaa
      961 tcgccgaccg tgggtatccg gcaaggatga tcctcagcgc ctataggccg acatcgtcaa
     1021 cctatgacga ggcgttttcc ccggacgggg ctctgcggcc gggctatgaa gccctgctcg
     1081 aggcccttgt cgaggccggc atccgcgaca cccaggatgc gcttcgacgc cggttgtcat
     1141 cgatggacgt cgatttcggg gacgacgaga gcaccttcga ggtcgatccg ctccctcgcc
     1201 tggtttcatc ggccgagtgg gaccgtctga gcgccggcct tgcgcaacgg cacgaggcga
     1261 tcaacgcttt catcggggac gcctatgggg accgctcgtt gatcgatcac ggtgtgatgc
     1321 ccgaccgcgt gctggaggaa tcggtctact acgaaccgat gatggaaggg ctgggtgtcg
     1381 tcgcgcaact cgccggcccc gacctgatcc gcgacgctac gggcgagatg ctcgtactcg
     1441 aagacaacgt acgaactcct tccggcatcg cctatgcggc ggccagtcgg agtgccctcc
     1501 gcagcgccgg gcagaacttc gtcgaaccgt ccatgctcga cccgtcgctg gcctacggac
     1561 tcctccacgc cgtacttcgg gacgcgaatc ccggcggcgg cgatccctcg attgtcatgc
     1621 tcagcgacgg accacggagc aaggcctggt tcgagcatcg ggagatctgc agccggttga
     1681 atatcccggt cgtggcaaca ggccagctcg aatcccgaag gaagcgccta ttcgcgctgg
     1741 tcggcggcga gcagctcgag gttcaagtgc tctacaaccg ctccagcgac gaaagcctgc
     1801 gcgcctcgga cggcgagctc tcccggctcg ggtcgctgct gaaagatccc ctggaagccg
     1861 gcaacgtgcg ttgcgtcaac agcttcggcg ccggcatcgc cgatgacaag gcagcccatt
     1921 gctacgtcga ggagatgatc cgcttctacc tgcgcgaacg gccactgctt cgttccgtgc
     1981 ccagcttcga cctcggagat ccggatcagc gggctaccgt catggaccgc ctcgatgagt
     2041 tcgtgatcaa gccccgatgg tcgttcggcg gccagggaat cgtgctcggc ccccgggcct
     2101 cggagctcga gttgcaggag atcagagctc gggtcgaacg catgccctcc ggctacgtgg
     2161 ctcagcagat gatcgagttc tcggtccatc cgaccgccgt tggagaccgg ctcgaaccgc
     2221 gccacgtcga tctccgggcc ttcgtggcaa cgctgaatgg caagtacacc gctcttccgg
     2281 tcgccctgac ccgtttcgcc ggcggcgccg gagatctggt cgtcaacagc actcagggtg
     2341 gcggtgccaa ggacacctgg ttgctgagat gagggcgacc ggagatacgc cgctgatcgg
     2401 aatctcggcg tccgagatac ggcccgctca gacgatccgc cagatccagg agggcgaacc
     2461 cttgagccgc gaagtcgctc tgggtctgga gtacgtgagc gcggtcatcg gggccggtgg
     2521 gctggcggtc gtcctgccgc cggccggcga ttgcctcgcc ggcgcccttc tcgatcggat
     2581 cgaaggcctg tgtctttcgg gcggcccgga tctcgacccg tcggcctacg gtgcatcccc
     2641 ccaccgatgc gccggtccgg ccgatccgac tctcgatcga ttcgagatcg acctcgctcg
     2701 ggaagcggtc cagcgggacc tgcctctgtt ggccgtctgc cgcggaatgc aggtattcaa
     2761 cgtgagccgt ggaggcacgc tgattcagca tctgccggac tgtgaatggg tcaccatcga
     2821 tcatcgccag gagaaagccg gcaatctccc cagccacggc gttcacctgg atccggcgag
     2881 ccggatctcg gaggtggtgg gcgagaacac gatcgccgtc aataccttcc accaccaggg
     2941 gatcgcctcg ctgggcgaag atctcgaagc ggtcggctgg gacgacgacg ggctcgtgga
     3001 ggcaatcgag atcccggatc gccgctttgc attgggcgtc caatggcacg ccgagttgat
     3061 ggccggccac gaggccgggg cgaccctgtt ctcgcggctc gtcgaatccg cccgcgagta
     3121 ccgtcgcaat catccggagc gggtgcactc gtgagcgatc ccgcccctcc cccgccggcc
     3181 tggtctgagt ggaagcagcg ggagcgcgct gagtacacca tcgggctcga ggaggaagtc
     3241 atgctgttga gcgacgactt ctcgctggcg aaccgggttg aggccgtgat gcctcgcctg
     3301 cccgagctgg tggccatcca ggccagtcga gagactcaga actcggtgat cgagctctgc
     3361 accgaggtgc acaccgacgt cggcggcgcc gtcgagcagc tgcgaagcct gcggaccgaa
     3421 ctgacggaca cgctcgccgc gttgcacctg agaggcgcgt gtgccggcac ccatccgttc
     3481 gcggtctggg aagagatcgc ggttaccagc ggcgatcgtc aagagctcgt ccacggatcg
     3541 atgcgtgagc tggcccggcg ggagcccacc ttcgccctgc atatccacgt cggcctcgcg
     3601 gatcccgagc ttgcgatcga tgtctacaac agcatgcggg tgcatttgcc cctgctgctt
     3661 gcgctctccg cgaattcgcc gtattggcag gggcgggaca ccggcctggc ctcggcgcgc
     3721 acaccgctct tccaggcatt tccgcgggtc gggatc
//
LOCUS       DASSGX010000067         2401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_773906, whole genome shotgun sequence.
ACCESSION   DASSGX010000067 DASSGX010000000
VERSION     DASSGX010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_773906"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="VFI12_01120"
     CDS             complement(<1..926)
                     /locus_tag="VFI12_01120"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7055026.1"
                     /translation="MTSSGAASSVSSQRGLIRLPSAVQFAGPMSGALGALILLSIYNA
                     FFTTNFLTLATLRVNLTQVSTIVLVAVGMTLVIATGGIDLSVGSLMAISGALAPLIFL
                     SDRGPLSSDWVAVPIAIVVPILATGILGLFNGMLVTRFDIQPIVATLVLFLGGRGIAK
                     ALLSGQLREFHKPSFDYIGTGRPLGIPVQVILMLTVVAVAAWAVRSTAFGRYVLATGG
                     NEAAARLAGVPIDRTRLAVYGITGLLSGLAGLIVISLNSSTDPNLVGMNMELDAIAAA
                     AVGGTVLTGGRATVVGTLVGALLVHQIRYTLLS"
     gene            complement(923..>2401)
                     /locus_tag="VFI12_01125"
     CDS             complement(923..>2401)
                     /locus_tag="VFI12_01125"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HET7055027.1"
                     /translation="LDGARLHVGQGEVHAIVGQNGAGKSTLMKVLNGAYRRDGGQIVL
                     DGRPVDFASPLQAQRNGVSTIYQEINLVPYRSVAENIFMGREPRRFGLINWPAMNRQA
                     EELLRQLNVHIDVRQPLMTYNVALQQMVAIARAVSFQSKVVIMDEPTSSLDDREVATL
                     FDVIRNLKATGVSVIYISHRMDELHQVCDRITIMRDGRTIDERLMANITKLEIVALML
                     GKDLGEVRQEGATGFHERKVAADTAPLLMANHVRRPRMLQDASVTVRPGEIVGLAGLL
                     GSGRTELARVIFGADRMESGEIAYAGKPGAFGSPADAIRAGIGLCSEDRKAEGIIPYL
                     SVRENLTLAALPLLTENGIVDADKQRAIVDRFIARLGIKTSNPDQPIRELSGGNQQKV
                     LLARWLCLNPKLLILDEPTRGIDVGAKAEIQALIDELADTGLGVLMISSEIEEITEGS
                     DRVTVLSEGVTVAELDKGQISQDAIMRAMAHGYGPGAEEPSA"
BASE COUNT          433 a          780 c          789 g          399 t
ORIGIN      
        1 gagaggagcg tgtagcgaat ctggtggacg agcagcgcgc cgaccagggt gccaacgacc
       61 gtggcccggc caccggtcag gaccgtgccg ccgacagccg ccgccgcgat cgcgtccagc
      121 tccatgttca tgccgaccag gttgggatcg gtcgacgagt tgagcgagat gacgatgagc
      181 ccggccaagc ccgaaaggag gccggtaatg ccatagaccg ccagccgggt gcggtcgatc
      241 gggacaccgg ccagccgcgc ggccgcttcg ttcccgccgg tcgccagcac gtagcggccg
      301 aacgcggtcg agcgcactgc ccaggccgca accgcaacca cggtcagcat caggatcacc
      361 tgaaccggga tgccgagcgg tcggccggta ccgatgtagt cgaagctggg cttgtgaaat
      421 tcgcggagct gcccggagag cagcgccttg gcaatgcccc gtccgcccag gaagagcacg
      481 agcgtagcga cgatcggctg gatgtcgaac ctggtgacga gcatcccgtt gaagagaccg
      541 aggattcccg tcgccaggat cgggaccacg atcgcgatcg ggacggccac ccagtccgag
      601 ctcagcggtc cccggtcgct caggaagatc aatggagcca gcgcgccgct gatcgccatc
      661 agcgagccaa cggagaggtc aatcccgccg gtggcgatga ccagcgtcat cccaaccgca
      721 accaggacga tcgtcgaaac ctgcgtcaga ttgacccgca gcgtggcgag cgtcaggaag
      781 ttggtcgtga agaaggcgtt gtagatcgag agcaggatca acgcgccgag cgcgccggac
      841 attggcccgg cgaactgcac ggccgaggga agtcgaatca gaccgcgttg ggaagagacg
      901 gagctagccg caccggagct ggtcatgcgc tcggctcctc cgcgcccggc ccatagccgt
      961 gtgccatcgc ccgcatgatc gcgtcctggc tgatctgccc cttgtcgagc tcggcgacgg
     1021 tgacgccctc gctcaggacg gtcacgcggt cgctgccttc cgtgatttcc tcaatttcgg
     1081 atgagatcat caggacgccg aggccggtat cggctagctc gtcgatcaat gcctggatct
     1141 cggcctttgc gccgacatcg atgccccggg tcggttcatc aaggatcagc agcttcggat
     1201 tcaggcacag ccagcgcgcc agcagcactt tctgctggtt tccgcccgag agctcccgga
     1261 tgggctgatc ggggttcgag gtcttgatcc cgagccgcgc gatgaaccga tcaacgatgg
     1321 cgcgctgctt gtccgcgtcg acgatgccgt tctcggtcag gaggggcagg gcggccagcg
     1381 tcaggttctc ccggaccgac aggtagggga tgatcccctc ggccttacgg tcctcagagc
     1441 agagcccgat cccggcccga attgcgtcgg ccgggctgcc aaatgcgccg ggcttgccgg
     1501 cgtacgcgat ctcgccagac tccattcgat cggcgccgaa gatgacgcgg gccaactccg
     1561 tccgtccgga cccgagcagc ccagccaggc cgacgatctc gccgggccga accgtgacgg
     1621 aggcatcctg gagcatgcgc ggtcgccgga catggttagc catgagcagg ggcgccgtat
     1681 cggcggcaac tttccgctcg tggaaaccgg ttgcgccctc ctgccgcacc tcgccgagat
     1741 ccttgccaag catcagcgcg acgatctcaa gcttcgttat gttggccatc agccgctcgt
     1801 cgatcgtgcg gccatcccgc atgatcgtga tccggtcgca gacctgatgc aactcgtcca
     1861 tccggtgcga gatgtagatg acactgacgc cggtggcttt cagattccga atgacgtcga
     1921 acagggtcgc gacctcgcgg tcgtcgagcg atgaggtcgg ctcatccatg atcacgacct
     1981 tgctctgaaa agagacggcg cgggcgatcg ccaccatctg ctggagggct acgttgtagg
     2041 tcatcaacgg ctggcgaaca tcgatgtgga cgttgagttg gcggagcagc tcctccgcct
     2101 gccggttcat cgccggccag ttgatcaggc cgaaccggcg cggctcccgg cccatgaaga
     2161 tgttctcggc cactgaccgg taggggacaa ggttgatttc ctggtagatc gtgctgacgc
     2221 cattgcgttg ggcctggagc ggtgaggcaa aatcgaccgg cctgccgtcg aggacgatct
     2281 ggcccccatc ccggcgatac gctccgttca gcaccttcat cagggtcgac ttgccggcgc
     2341 cgttctggcc cacgatcgcg tggacttcgc cttggcccac atggagccgg gcgccatcaa
     2401 g
//
LOCUS       DASSGX010000068         3928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1426977, whole genome shotgun sequence.
ACCESSION   DASSGX010000068 DASSGX010000000
VERSION     DASSGX010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1426977"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VFI12_01130"
     CDS             <1..285
                     /locus_tag="VFI12_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7055028.1"
                     /translation="DTQCGFKAFTRFASQEITRRARLYADHETITGARVTAFDVEMLV
                     IARKLGLKITEIPVIWTYGTQSKVNPITDTVTNLRDIASIKLNALKHAYD"
     gene            435..1148
                     /locus_tag="VFI12_01135"
     CDS             435..1148
                     /locus_tag="VFI12_01135"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HET7055029.1"
                     /translation="MEGDMREFPLAEILQFISLGSRSGILEIVRRDGVYGIIFTSGTI
                     TGLTADGWTLANELRESQLLPDNILTGLLAVNGNIQDLRAAILTGGYMTADEWTAFVS
                     RQVERLLYALFDVRDGKFRFRQSAPPEGQWLPVRVSADRAVLEGTRWSEAWARASEAI
                     PSRQSRFARTGRVPRANVRVTPTQWRVFVSTNEPGTLTQIAARSILSEVDVIESLQYL
                     LDLNLVTRVDTGGIATAIR"
     gene            1442..2431
                     /locus_tag="VFI12_01140"
     CDS             1442..2431
                     /locus_tag="VFI12_01140"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET7055030.1"
                     /translation="MNRLVERAEAINNDAAGVREELEGFLEQFALESGMAGGTLRQAT
                     FAGGVIDIESMRADVVTMALNASKAAILTSSLVSVRDSIDNAARHFASDAALTVSLDA
                     TFDHFQAAIIEAREDERRKLAREIHDGPAQVLTNAVYLVQIMEQIVKRNPEAVEEELG
                     RLREHLKEGGTEIRRFMSDLRPIALKDYGLAIYLERYVDNYARFFGRRAKCTIPGTLP
                     VLTEDQEFMIFRIVQEALQNVHKHGGNDASVDISISMDDGDLVVRVSDDGVGFDPALV
                     SPKLSSGAGLLGMRDRANVSRARLNIESRLGSGTVITLSLPVDAQSDLGEEPS"
     gene            2455..3159
                     /locus_tag="VFI12_01145"
     CDS             2455..3159
                     /locus_tag="VFI12_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7055031.1"
                     /translation="MNRIQVLIVDDHPLFRRGVRWGLEAEPDILVVGEATDGQMAVQQ
                     ADLLVPDVVLIDINMPQMNGLEVTRIIRRRHPQTGVIVLTMHEDDEQLFHAIRVGAAA
                     YCTKDAQQEEIIKLIRRVARGEYLINENVLQRPFVASRVLDQFRELSQMETGADGVFS
                     PLTPREVEILDCVAQGNSNKEIARILNISDQTVKNHITSILRKLAVNDRTQAVIYALR
                     HGWIRLTDDSSAALNN"
     gene            complement(3221..>3928)
                     /locus_tag="VFI12_01150"
     CDS             complement(3221..>3928)
                     /locus_tag="VFI12_01150"
                     /inference="COORDINATES: protein motif:HMM:NF020260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055032.1"
                     /translation="RVSFAIPFAPPAAPVQPTLMPSPAANSIRRDADEPAHTTPPAGS
                     RSVPMLRVLGQVGASYIIAEGPDGLYLIDQHAAHERVMYEKIRSQLVSQTADQQPLLD
                     PMVIELDPHEAAVMERSLAELNQIGFEIEPFGPQSFTLRTIPALMAGVDIRERMHLIL
                     RELADGGVGESWLDSVAISAACHTSIRAGQVLSIAEMRELVNQLEQTEHPRACGHGRP
                     TMLLMSQIDLEKQFARR"
BASE COUNT          777 a         1238 c         1202 g          711 t
ORIGIN      
        1 gacacccaat gcggcttcaa ggcattcacg cgcttcgcca gccaggagat cacgcgccgg
       61 gcccggctgt acgcggacca cgaaacaatc accggcgccc gggtcaccgc cttcgacgtt
      121 gaaatgctcg tcatcgcgcg caagcttggg ctgaagatca ccgagattcc ggtgatctgg
      181 acctacggca cgcagagcaa ggtcaacccg atcaccgata cggtcacgaa cctcagggac
      241 atcgcgtcca tcaaactgaa cgcgctcaag cacgcctacg actagcacga acaggcccga
      301 accgcgcgtc ctgttttcgg ccaggactcg catgaccaaa aggttatgga gcggggcaat
      361 tccgcccggt acccttgaat cgcctggcaa atgccggccc tgttgaaccg tctgaacacc
      421 gtgggaatgc gaacgtggaa ggcgacatgc gggagttccc gctcgcagag atcttgcagt
      481 tcatctccct cggatcgcga tccggcatcc tcgaaattgt ccgcagggac ggggtctacg
      541 gcatcatctt cacgtcgggg acgatcacag gcctgaccgc ggacggttgg accctcgcca
      601 acgagcttcg ggagtcccag ctccttccgg acaacatcct gacggggctg ctggcggtca
      661 acggcaatat ccaggacctc cgcgccgcca tcctcacggg cggatacatg accgccgatg
      721 aatggaccgc gttcgtctct cgccaggtgg aacgcctgct ctatgcgctt ttcgatgtcc
      781 gggatggcaa gtttcgcttc cgccagtcag cgccgcccga gggccaatgg ctccccgtgc
      841 gcgtgtccgc ggaccgcgcc gtcctcgagg gcactcgctg gagcgaagca tgggcccgcg
      901 cgtcggaggc tatcccctca cgccagagcc gattcgcgcg aaccggccgc gttccgcgcg
      961 ccaatgtccg ggtgacgccg acccaatggc gcgtattcgt ctccacgaac gaaccgggca
     1021 cactgacgca aatcgccgcc cgttcgatcc tctccgaagt ggacgtgatt gaatcgctcc
     1081 agtacctgct cgacctgaac ctggtgacac gggtcgatac gggcgggatc gcaacggcaa
     1141 tccgctaaga cccctcgaca ataagacaca ggtttggtac tgtacgtacg cattgttcgt
     1201 tctacttacg aacgtgggat acgggacttt ttggttacct tgcggcgtga acggccgccg
     1261 ggccgaaggt accgttctct catacccgcc ctcggtcctc ccgtagcgac cgtcgtgacc
     1321 agcgttggag tccatgagga cgcgctcaat cgtgacgtca gctgaaatgc aatcaacgct
     1381 tcgagagctc gtggaaatcc tgggcgaggc agagtccttc gcgcgcggag cactggtcga
     1441 gatgaatcga ctggttgaac gtgcggaagc aatcaacaac gacgccgccg gtgtccgcga
     1501 agagctcgag gggttcctcg aacaattcgc gctggaatcc ggaatggcgg gtggcacgct
     1561 caggcaagca acctttgccg gcggcgtgat cgacatcgaa tcaatgcgtg ccgatgtcgt
     1621 gacgatggcg ctcaacgcat ccaaggcggc aatcttgacc agcagcctgg tgtcagtacg
     1681 agattcaatc gacaacgcgg cgcgccactt tgccagcgac gccgccctga ctgtctcact
     1741 cgatgcgacg ttcgatcatt tccaggcagc gatcatcgag gcacgcgagg acgagcggcg
     1801 aaagttggcg cgggaaatac acgacggacc ggcgcaggtc ctcacgaatg cggtctacct
     1861 cgtccaaatc atggagcaga tcgtcaagcg aaatccggaa gccgtcgagg aagagctcgg
     1921 ccggctccgg gagcatctca aggaaggcgg gacggagatc cggcgattca tgtctgatct
     1981 ccgccccatc gcgctcaagg attacggtct cgccatctac ctggagcgat atgtcgacaa
     2041 ctacgcgcgg ttctttggtc gccgggccaa atgcacgatc cctggtaccc tgcccgtcct
     2101 gaccgaagat caggagttca tgatcttccg aattgtccag gaggcgctcc agaacgtgca
     2161 caagcacggt ggcaatgacg cttcggtcga catcagcatt agcatggacg acggcgacct
     2221 ggtcgtgcgc gtctccgacg acggggttgg attcgatcca gccctcgttt cacccaagct
     2281 cagctcgggc gccggcctcc ttgggatgcg ggaccgcgcg aacgtatcgc gcgcccggct
     2341 gaacatcgag agccgtcttg gttccggcac ggtcattaca ctctcgcttc ccgtagacgc
     2401 ccagtcggac ctgggagagg agccctcata atggatgagc cgaggagaag ttcaatgaat
     2461 cgcatccagg tgttgatcgt cgatgatcat cccctgttcc gccgcggcgt ccgctgggga
     2521 ctggaggcgg agccggacat cctcgtcgtg ggcgaggcaa ccgatggcca gatggcggtg
     2581 caacaagccg acttgctcgt gccagatgtc gtcctgatcg acatcaacat gccgcagatg
     2641 aacggcctcg aggtaacccg gatcattcgt cgccgtcatc cgcaaaccgg cgtgatcgtc
     2701 ctcaccatgc acgaggacga tgagcaactc ttccacgcga tccgcgtcgg cgcggccgcc
     2761 tactgcacga aggacgcgca gcaggaagag atcatcaagc tgattcgtcg ggttgcgcgc
     2821 ggcgagtacc tgatcaatga gaatgtgctg cagcggccgt tcgtcgcatc ccgggtgctc
     2881 gaccagttcc gcgagttgtc gcaaatggaa accggcgctg acggcgtctt ctcgccgctc
     2941 acgccgcgcg aggttgagat cctcgactgc gttgcccagg gaaacagcaa caaggaaatc
     3001 gcgcggatcc tcaacatctc ggatcagacg gtcaagaacc acattacgtc aattcttcgc
     3061 aagttggcgg tcaacgaccg cacccaggct gtcatctacg cgctccggca cgggtggatt
     3121 cgcctgacgg acgactcgtc cgccgcgctg aacaactgag ctcacttccg cgattggctg
     3181 ggagaaacgg atcggtgttg caccgatccg tttctcgttg ctaccgtcga gcgaattgct
     3241 tttcgagatc gatttggctc atcaggagca tcgtcggccg gccatggccg caagcgcggg
     3301 gatgctccgt ctgctccaac tgattgacga gctcccgcat ctcggcaatc gaaagcacct
     3361 ggccggcccg gatcgatgtg tggcaggcgg cgctgattgc gaccgaatcg agccacgact
     3421 cccccactcc gccgtcggca agctcgcgca ggatcaggtg cattcgctct cgaatgtcga
     3481 cgccggccat gagcgcgggg atcgtccgga gcgtgaatga ctgcggcccg aaaggctcga
     3541 tctcgaatcc gatctggttc agctcggcga gcgagcgctc cataacggcc gcctcgtgcg
     3601 gatcgagctc gatcaccatc gggtcgagta gcggctgttg atcggccgtc tgcgagacga
     3661 gctgcgaacg gatcttctcg tacatcaccc gttcgtgcgc ggcatgttga tcgatcaggt
     3721 acagcccatc cggcccctcc gcgatgatgt acgacgcgcc gacctgcccg agcacacgga
     3781 gcatcggcac cgatcgcgag ccagcgggag gggttgtgtg tgcgggttca tccgcgtcgc
     3841 gacgtatcga gttggcggcg ggtgacggca tgagcgtcgg ctgaaccggc gcggccggcg
     3901 gagcgaatgg gatcgcgaat gagactct
//
LOCUS       DASSGX010000069         4839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_12625, whole genome shotgun sequence.
ACCESSION   DASSGX010000069 DASSGX010000000
VERSION     DASSGX010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4839
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12625"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VFI12_01155"
     CDS             complement(<1..627)
                     /locus_tag="VFI12_01155"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HET7055033.1"
                     /translation="MASTTTEERPIGEFQAISLDGVGVVEYTQDDATTLKIEAEPEAM
                     ALISTEVVDGVLRIQTQLLTVKAIHLKHPPVYTVTGPTLNALDLRGAGRAKIGRLKAD
                     QLVVTVDGVGDVRMTNQTLGTCRIKIAGAGHIQAAGIATVQEISITGTGSFSGDQFVG
                     ETAKVNIAGAGRAKVHATQDLAVDIGGVGSVTYVGDPRITPNVGGLGRV"
     gene            complement(662..1390)
                     /locus_tag="VFI12_01160"
     CDS             complement(662..1390)
                     /locus_tag="VFI12_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055034.1"
                     /translation="MKFTPIATTTVLAAAGGIAFTSVVRPWYHRWGASDEEVMRPMPL
                     DERIPGPTMQTTMAITINARPEAIWPWLVQIGDPPRAGYYSYTWIEKAVGLDIQNAGR
                     ILPRFQSVTVGQALDGNGTMVVQAVEPGKSLVLGPPDSIDYLKCTWAFGLYPIDARST
                     RLVTRVRARWSYRQTLCTTPPWAWPMWLLIDPGAFIMERKMLKEIKGLAEAHSPILQS
                     GVVITEPAMARMETDAAVDAVVPG"
     gene            1584..2960
                     /locus_tag="VFI12_01165"
     CDS             1584..2960
                     /locus_tag="VFI12_01165"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 family protein"
                     /protein_id="HET7055035.1"
                     /translation="MRIRETVRRAFASFLALPTAVIACFGLLAIGTYWLDRSGWSWLS
                     PIRDFMRDHVFGSEQSTANLLATIAGGVITITSITFSLLLLALQQSAGALTHDVFDQF
                     LRRRANQVYFGFFVGLTLYSLIVLISVDPPFNPVIGASLALVLTIVALYILLLLIYAC
                     ISQMRPNEIVRAIHDMTLKARDRQQPLIMRTRRSATLSQMPATVVRASRDGFVSGIDL
                     DAIAAGLDQARGSVEVSYLQSIGSYVAFQDPIAEIRGERGEELAALGDCVAGATAIQQ
                     DRDVDTDPAYGIDQLATIAWRSISTSQQNPSPGIAAVNALHDLLARWSTDDDPKVDDP
                     PLPVVYPDNVVGSALGALESLTVVASESMQHQTYAAILNAIAVLFERLCPAERQRAED
                     LVLRSLSGLGDQILTRALDDALAALAESLDEGGALDTARAVRVARNQLAESIGRLNSR
                     STRVPQSM"
     gene            complement(2971..3195)
                     /locus_tag="VFI12_01170"
     CDS             complement(2971..3195)
                     /locus_tag="VFI12_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055036.1"
                     /translation="MTDPDVQRELEANKQRARRINEQAKEQNRSPDDPTSLIDDAYQA
                     FVEPPLLSDDLDPQEAERRAIQNDEAARET"
     gene            complement(3279..3617)
                     /locus_tag="VFI12_01175"
     CDS             complement(3279..3617)
                     /locus_tag="VFI12_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004111837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HET7055037.1"
                     /translation="MLMTTTSTLDGMPIQRYLGVVSGEAILGANIMKDLFASVRDIVG
                     GRSGAYEAELIRAKETALREMESNAAAMGANAIIGIDLDYETLGSGNSMLMVCVSGTA
                     VIVNQAGRTA"
     gene            complement(3673..4494)
                     /locus_tag="VFI12_01180"
     CDS             complement(3673..4494)
                     /locus_tag="VFI12_01180"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7055038.1"
                     /translation="MTEASARRPADVWASGDAYEPYVGRWSRIVARDFVAWLAVPPGS
                     RWLDVGCGTGAISQSILDIAQPAGIVAVDPSEAYTAYARRRFEGAPVEVRIGDARALP
                     VADGTFDAVVCGLMLNFVPAADQPKAAAGMVRAARPGGTVGAYVWDYAGEMQFMRHFW
                     DAAVALDPENEDLDEGPRFPICQPEPLAALYRDAGLANVEVRAIDIPTIFRDFDDYWT
                     PFLGGQGAAPAYAMALTEEHRAALRDRIRAGLPFAPDGSIHLIARVWAVRGAVVE"
     gene            4655..>4839
                     /locus_tag="VFI12_01185"
     CDS             4655..>4839
                     /locus_tag="VFI12_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055039.1"
                     /translation="MSSAPEPAVAGFTLLATKLYVPKWRSGLVSRPRLVERLCQGAER
                     TLTLVTAPAGFGKSTLLA"
BASE COUNT          702 a         1622 c         1652 g          863 t
ORIGIN      
        1 gacgcggccc aggccgccga cgtttggggt gattctgggg tcgccgacgt aggtgacgga
       61 gccgacgccg ccgatgtcga ccgccaggtc ctgggtggcg tggaccttgg cccggccggc
      121 gcccgcgatg ttgaccttgg ccgtctcccc gacgaactgg tcgccggaga agctgccggt
      181 accggtgatg ctgatctcct ggaccgtcgc gatcccggcc gcctggatgt ggcccgcgcc
      241 ggcgatcttg atccggcagg tcccgagcgt ctggttggtc attcgcacgt ccccgactcc
      301 atcgacggtg accacgagct gatcggcctt gaggcggccg atcttcgccc ggcccgcgcc
      361 acgcagatcg agcgcgttca gggtcggccc ggtgacggtg tagaccggcg gatgcttgag
      421 gtggatcgcc ttgaccgtca gcagctgagt ctggatccga agcacgccat cgacaacctc
      481 ggtcgagatc agcgccatgg cttccggctc ggcttcgatc ttgagggttg tcgcgtcgtc
      541 ctgcgtgtac tcgacgacgc cgaccccatc gagtgagatt gcctggaact cgccgattgg
      601 tcgttcttct gtggtcgtgg atgccatggg ttcctccgtt cgcggggtag cgggcgccgg
      661 ctcatccggg gacgacggcg tcgacggctg cgtctgtctc cattctagcc atggccggtt
      721 cggtgatgac cacgccggat tgcaggatcg gtgaatgcgc ctcggccagc cccttgatct
      781 ccttcagcat cttccgctcc atgatgaagg cgccggggtc gatcagcagc cacatcggcc
      841 aagcccacgg cggcgtggtg cacagggtct ggcggtagct ccagcgcgcc cggacccgcg
      901 tgaccagccg ggtcgagcgc gcgtcgatcg ggtagagtcc gaaggcccac gtgcatttga
      961 ggtagtcgat cgaatcgggt gggccgagga cgagcgattt cccgggctcg accgcctgca
     1021 cgaccatcgt gccgttcccg tccagcgcct gcccgaccgt gaccgactgg aatcggggca
     1081 ggatccggcc ggcgttctgg atgtcgaggc cgaccgcctt ctcgatccac gtgtagctgt
     1141 agtagccggc gcggggcgga tcgccgatct ggaccagcca cggccagatt gcctccggcc
     1201 gggcattgat cgtgatcgcc atcgtggtct gcatggtcgg gcccgggatg cgctcgtcga
     1261 ggggcatcgg ccgcatgact tcctcgtcgg acgctcccca gcggtggtac cagggccgga
     1321 cgaccgaggt gaaggcgatt ccccccgcag cggccaggac ggtcgtcgtg gcaatcggcg
     1381 tgaacttcat cgcagcctcc tcccggctgc ttcgctcgat ggagcagcct tgctgcacga
     1441 tatggcccat cgagaccggc cggtgaccta cttcgtgccg cgagcatcac aggattgcaa
     1501 cggtccggcg tagcgctttg ctcgcggagc gatcgatcca tcggcaagac tggcagcggg
     1561 atcgaagacg tggggaggaa tcgatgcgga tccgggagac ggtacggcga gcgttcgcct
     1621 cgttcctggc gctgcccacg gcggtcatcg cctgcttcgg actccttgcg atcggcacct
     1681 attggctcga tcgatcgggc tggagctggc tttcgccgat ccgtgatttc atgcgggacc
     1741 acgtcttcgg gagcgagcag tcgaccgcga acctgctggc gacgattgcc ggcggcgtga
     1801 tcacgatcac ctcgatcacc ttttccctgc tcctgctggc gctccagcaa tcggccggcg
     1861 cgctgacgca cgacgttttc gaccagttcc tgcgccgccg ggcgaaccag gtctatttcg
     1921 gtttcttcgt cggcctcacg ctctactcgc tgatcgtcct gatctcggtc gatccgcctt
     1981 tcaacccagt catcggcgcg agccttgcgc ttgtcctgac gattgtcgcg ctctacatcc
     2041 tgctcctgct gatctatgca tgcatcagcc agatgcggcc gaacgagatc gtccgcgcca
     2101 tccatgacat gacgctcaag gcccgcgatc gccagcaacc cctgatcatg cggaccaggc
     2161 gctccgccac cctctcgcag atgccggcga cggtggtgcg ggcatcgcgc gacggcttcg
     2221 tgagcgggat cgacctcgac gcgatcgcgg ccgggttgga tcaggcgcgg ggctcggttg
     2281 aggtcagcta cttgcagtcg atcggctcgt acgtcgcctt ccaggatccg atcgccgaga
     2341 tccggggcga gcgcggcgag gagttggcgg cactcggaga ctgcgtggcc ggcgcgaccg
     2401 ccatccagca ggaccgggac gtcgacaccg acccggccta cggcatcgac cagctcgcga
     2461 cgatcgcgtg gcgctcgatc tcgacctcgc agcagaatcc gtcgccgggg atcgccgcgg
     2521 tcaacgcgct gcacgacctg ctcgcccggt ggagcacgga cgatgacccg aaagtggatg
     2581 atccgcccct gccggtggtc tacccggaca atgtcgtggg ttcggcgctc ggggcgctgg
     2641 aatcactgac ggtcgtggca tcggagtcga tgcagcacca gacgtatgcg gcgatcctga
     2701 acgcgatcgc ggtcctgttc gagcgattgt gcccggccga gcggcagcgg gcggaggatc
     2761 tggtgttgcg gtcactctcg gggctgggcg atcagatcct gacccgagcc ctggacgatg
     2821 cccttgccgc gctcgcggag tcgctggacg agggtggagc gctggatacg gcgcgggcgg
     2881 tccgggtcgc ccggaatcag ctggcggaga gtatcggccg cctcaattcc cgctccaccc
     2941 gcgtgcccca atcaatgtag ggacacggaa ttatgtctct cgcgcggcct cgtcgttctg
     3001 gatggcgcgg cgctcggctt cctgcgggtc gaggtcgtcg ctgaggagcg gcggctcgac
     3061 gaaggcctgg taggcgtcgt cgatcagcga cgtcgggtcg tccggcgacc ggttctgctc
     3121 cttcgcctgc tcgttgatac ggcgtgctcg ctgcttgttg gcctccagct cgcgctgcac
     3181 atcgggatcg gtcattgtcc tgcctccttc gtatggttcg gcaatgcggc catgctaacg
     3241 aggctggctg gggaatccgt ggcgaccggc tacatggctc aggcggtgcg gccggcctgg
     3301 ttgacgatga cggcggtgcc gctgacgcag accatcagca tgctgttgcc ggagccgagc
     3361 gtctcgtagt cgaggtcgat gccgatgatc gcgttcgcgc ccatggcggc ggcgttcgac
     3421 tccatctcgc gcagggcggt ctccttggcc cggatcagct ccgcctcgta cgcgccggag
     3481 cggccgccga cgatgtcgcg cacgctcgcg aagaggtcct tcatgatgtt cgcgcccagg
     3541 atggcttcgc cggagacgac gccgaggtag cgctggatgg gcatcccgtc gagtgtgctc
     3601 gtggtggtca tcaacatcgg tggctccttc ccttcctgtc tatgcatcga ttgcagggcg
     3661 atccgggcac gcttattcga cgacggcgcc gcgtacggcc cagacccggg cgatcaggtg
     3721 aatcgatccg tccggtgcga aggggaggcc ggcccggatg cgatcccgca gggccgcgcg
     3781 gtgctcctca gtcagggcca tcgcatacgc cggggccgcg ccctggccgc cgagaaaggg
     3841 cgtccagtaa tcgtcgaagt cgcggaagat ggtcgggatg tcgatcgcgc ggacctcgac
     3901 gttcgccagg ccggcgtcgc gatagagcgc ggcgagcggc tcgggctggc agatcgggaa
     3961 gcgcggcccc tcgtcgagat cctcgttctc cgggtcgagg gcaacggccg cgtcccagaa
     4021 gtggcgcatg aactgcatct cgccggcgta gtcccagaca tacgcgccga ccgtcccgcc
     4081 cggccgcgcg gcccggacca tcccggcggc ggctttcggc tgatcggccg ccggcacgaa
     4141 gttgagcatc agcccgcaga cgacggcgtc gaacgttccg tccgctactg gcaaggcccg
     4201 ggcatcgccg atcctgacct cgaccggcgc gccttcgaag cggcggcggg cgtaggcggt
     4261 gtaggcctcg gagggatcga ccgcgacaat gcccgccggc tgggcgatgt cgaggatcga
     4321 ctggctgatc gctccggtgc cgcagccgac atcgagccac cggctgcccg gcgggaccgc
     4381 gagccaggcg acgaaatccc gcgccacgat ccggctccag cggccgacat acggctcgta
     4441 ggcatcgccg ctcgcccaca cgtcagccgg tcgccgcgca ctcgcctccg tcatcgcact
     4501 ccctctcccc gatctcgcga agatgacgtc tcctgttgcc cttccttgac gattatgcgc
     4561 ggtatcggca agatgaccgc gcgtagcgtg cagaacgagc ccgaacggag ccggtgcccc
     4621 ggctgcgggt gcgtgtcgat ggagcaaggt ccccgtgagc agcgcgccag aaccagccgt
     4681 cgccggattc acgctcctcg cgaccaagct ctacgtcccc aaatggcgtt cgggcctggt
     4741 ctcgcggccc cgtttggtcg agcgactgtg ccagggcgcc gagcgcacgc tgacccttgt
     4801 caccgcgccg gctggcttcg gcaagtcgac cctgctcgc
//
LOCUS       DASSGX010000070         3139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_12948, whole genome shotgun sequence.
ACCESSION   DASSGX010000070 DASSGX010000000
VERSION     DASSGX010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12948"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /gene="hslV"
                     /locus_tag="VFI12_01190"
     CDS             <1..605
                     /gene="hslV"
                     /locus_tag="VFI12_01190"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HET7055040.1"
                     /translation="RPHATTIIAVLREDGVAMAGDGQVTFGEVVMKHGARKIRTLQDG
                     TILAGFAGAVADALTLIEKFEAQLKAWDGNMRRAAVELAKEWRTDRYLRRLEAQLLIA
                     EVGSLLLLSGDGDVIEPDDGIAAIGSGAPYATAAARALSRHSELGAKDVAMAAMEIAA
                     DLCIYTNDHFSMEWIPADGTESGETTESDAEAEIERVTNG"
     gene            598..2076
                     /gene="hslU"
                     /locus_tag="VFI12_01195"
     CDS             598..2076
                     /gene="hslU"
                     /locus_tag="VFI12_01195"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HET7055041.1"
                     /translation="MAEKAPVQAGGAPGTRSTSAQNAPKLENLTPAKMVAELDKYIVG
                     QVDAKRAVAVALRNRYRRQLLSEDLRREVHPKNILMIGPTGVGKTEVARRVARIVDAP
                     FIKVEATKFTEVGYVGRDVESIVRDLVEVAVSNLHSKRLEQVNDEAANLASQRLVDLI
                     ADQTILKRAARPRRVRGAENGDAATDELAERRKARERKRIVRMLAEKQLEDETIEIEI
                     EENGFGGEYGSSDFGDSAPSEDSYDSVQDMVDIFASMLPRRSFRRRVPVREARRILTQ
                     QEANRLIDFDAVVDEAIQRAEDAGVVFLDEIDKTIPSNSEYGADVSAEGVQRDLLPIV
                     EGSVVMTRYGPVRTDHVLFIAAGSFHQAKPSDLIPELQGRFPIRVELQSLTEDDLLRI
                     LTEPENALTKQYSALLATEGVELVFQKDGLKEMARLATLVNARNEDIGARRLQTIIEK
                     VVEEISFDADQYVGQSLTIDAPFVLERVGEVAQDDDLSNFIL"
     gene            2212..>3139
                     /locus_tag="VFI12_01200"
     CDS             2212..>3139
                     /locus_tag="VFI12_01200"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7055042.1"
                     /translation="MLAFAIKRILWLIPTLLVVTLLTYFIMELTPGSPFDLANANGIT
                     PEMIARLEHQYGLDKPWYERYVIYVGNAVRGNFGESYSYRPQQVSDIIGRTLPTSLWL
                     GTMATLFAVILGMILGILAAVNQNGIIDYVTVTIAILFYSMPNFVMGFILILLFAIWL
                     PDRGIDLGFRPSGWEGPKDWILPTIALGAAPLATIARYTRSSMIDVIRSDYVRTARAK
                     GLAQRKVILKHVLKNALIPVVTLIGPIFAIVGTGSFFVEKIFNVPGMGKFYIESMQTK
                     DQTMILAVVLIFGVFLAVMNIMVDIVYGLIDPR"
BASE COUNT          623 a          972 c          991 g          553 t
ORIGIN      
        1 tgcgtcccca tgccaccacg atcatcgccg tcctccgcga ggacggcgtc gccatggccg
       61 gcgacggcca ggtgacgttc ggtgaagtgg tcatgaaaca cggcgcccgc aagatccgga
      121 cgctgcagga cggaacgatc ctggcgggat tcgccggcgc ggttgccgac gccctgactc
      181 tgatcgaaaa gttcgaagcg cagctcaagg cgtgggacgg caatatgcgc cgcgcggctg
      241 tcgagctggc caaggagtgg cgcactgatc gctatctgcg gcggctcgaa gcccaactcc
      301 tgatcgccga ggtcggctcc ctgctgttac tctccggcga cggggacgtg atcgagccgg
      361 atgacgggat cgccgcgatc ggctccggtg cgccatacgc gacggcggcg gcacgagcgc
      421 tttcgcggca ttctgagttg ggcgcgaagg atgtcgcgat ggcggcgatg gagattgccg
      481 cggacctgtg catctatacc aacgaccact tcagcatgga gtggattccc gccgacggca
      541 ccgagtccgg cgaaacgacg gaatcggacg ccgaggctga gatagaacga gttaccaatg
      601 gctgagaaag cacctgtcca ggcaggcggc gcaccgggta cgcgctcgac cagcgcgcaa
      661 aacgcaccga agctggagaa cctgacgccg gcaaaaatgg tggccgaact cgacaaatac
      721 atagtcgggc aggttgacgc caagcgtgcc gtcgccgttg cgctccgcaa ccgataccgg
      781 cgccagctcc tctccgaaga cttgcggcgc gaggtgcatc ccaagaacat cctgatgatc
      841 ggcccaaccg gcgttggcaa gaccgaggtc gcgcgccgtg tggcaaggat cgtcgatgcc
      901 ccgttcatca aggtcgaagc gacgaagttc accgaagtcg gctatgtggg acgcgatgtc
      961 gagtcgatcg tccgcgacct ggtcgaagtc gcggtcagca acctgcatag caaacgactg
     1021 gagcaagtga atgacgaggc ggccaacctt gcgtcgcagc gtcttgtcga tctcatcgcc
     1081 gatcagacga tcctgaaacg ggcggctcgt ccgcgccgcg tacgaggcgc cgagaacggc
     1141 gatgccgcaa ccgacgaatt ggccgagcgc cgcaaggcca gggagcggaa gcggatcgtg
     1201 cggatgctgg ccgagaagca gctcgaggac gagacgatcg aaatcgagat cgaggaaaac
     1261 gggttcggcg gcgagtatgg cagctccgat ttcggagact ccgcaccgtc cgaagactcg
     1321 tacgattccg tgcaggatat ggtcgacatc ttcgcgagca tgctgccgcg ccgatcgttc
     1381 cggcgccgcg taccggtgcg cgaagctcgt cggatcctga cccagcagga ggccaaccgc
     1441 ctgattgact tcgacgcggt ggtggacgag gcaattcagc gggcagagga cgccggcgtg
     1501 gtcttcctcg acgagatcga caagacgatt ccctcgaact ccgaatacgg cgccgacgtg
     1561 tcagccgaag gggttcagcg cgatctgctc ccgatcgtcg agggctccgt cgtgatgacc
     1621 cggtatgggc cggtgcgcac ggatcatgtc ctcttcatcg cggctggctc gttccaccag
     1681 gcgaagccgt cggacctcat tcccgagctc cagggccgct tcccgatccg ggtcgaactg
     1741 cagagcttga ccgaggacga cctgctccgg atcctgaccg agccagaaaa cgcgctgaca
     1801 aagcagtaca gcgcgttgct tgcgaccgag ggcgtcgagc tcgtcttcca gaaggacggg
     1861 ctcaaggaga tggccaggct cgcgacgctg gtcaacgcgc gcaacgagga tatcggcgca
     1921 cggcgcctgc agaccatcat cgaaaaggtg gtcgaggaga tctccttcga tgccgatcag
     1981 tacgttggac aatcgctgac catcgatgcc ccattcgtgc tcgagcgcgt gggcgaagta
     2041 gcacaggatg acgatttgag caacttcatt ctctaggccg gagatgcgcc accacctgcc
     2101 ggtttatgtg ttgacacccc gattgagcgc gactatattg ctgccgccta tacaagagcg
     2161 caaagagcag tcgggcgagg accgtagccg ccgcccaccg gggtaagttg aatgctcgct
     2221 ttcgcaatca aacgcatcct ttggctgatc cccaccctgc tcgtggtgac gttgctgacg
     2281 tacttcatca tggagctgac gccgggcagc ccgttcgacc ttgcgaacgc caacggcatc
     2341 acgccggaga tgatcgcgcg cctggagcac cagtacggcc tcgacaagcc atggtacgag
     2401 cggtacgtga tctacgtggg gaatgccgtc cggggcaact tcggcgagtc gtattcctac
     2461 cggccgcagc aggtgagcga catcatcggt cgcacgctgc cgacgtcgct ctggcttggc
     2521 acgatggcca ccttgttcgc ggtgattctg ggcatgatcc tcggtatcct ggccgcggtg
     2581 aaccagaacg gcatcatcga ctatgtcacg gtcaccatcg cgatcctgtt ctacagcatg
     2641 cccaatttcg tgatgggctt catcctgatc ttgttattcg cgatctggtt accggatcgc
     2701 ggcatcgacc tcggctttcg gccgtccgga tgggagggcc cgaaggactg gattctgccg
     2761 accatcgccc tcggagcggc cccgctggca accattgccc ggtacactcg ctcgagcatg
     2821 atcgacgtga tccgctccga ctacgtgcgc accgcccgcg ccaagggtct ggcccagcgc
     2881 aaggtcatcc tcaagcatgt gctcaagaac gcgctgatcc cggtcgtaac gcttatcggc
     2941 ccaatcttcg cgatcgtcgg caccggctcc ttcttcgtcg agaaaatctt caacgtgccg
     3001 ggcatgggca agttctatat cgaaagcatg cagaccaagg accagaccat gatcctggcc
     3061 gtcgtgctca tcttcggagt cttcctggcg gtgatgaaca tcatggtcga catcgtgtac
     3121 gggctgatcg acccgcgaa
//
LOCUS       DASSGX010000071         2585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_883737, whole genome shotgun sequence.
ACCESSION   DASSGX010000071 DASSGX010000000
VERSION     DASSGX010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883737"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..690)
                     /gene="leuD"
                     /locus_tag="VFI12_01205"
     CDS             complement(79..690)
                     /gene="leuD"
                     /locus_tag="VFI12_01205"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF002458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HET7055043.1"
                     /translation="MQAVRKITGIVAPLDRSDVDTDQIIPASYLKRVERTGFGEGLFA
                     SWRQSDPDFVLNKKEFQNAKILVAGPNFGTGSSREHAVWALMDYGIQAVVSPRFGDIF
                     RNNSTKTGLIPVQVDQDVADLLLKAASEEPGLQMTIDVANRTLSVPEIGVEVEFPMDD
                     FTQHRLLNGLDDIGLTLQHQDDIAAFEAHRPGFLPVTIGGEAR"
     gene            complement(694..2103)
                     /gene="leuC"
                     /locus_tag="VFI12_01210"
     CDS             complement(694..2103)
                     /gene="leuC"
                     /locus_tag="VFI12_01210"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF004016.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HET7055044.1"
                     /translation="MTTQPRTLADKVWEQHVVRSAPGEPDLLYIDLHLVHEVTSPQAF
                     DGLRMAGRPVRRPDLTIATADHNVPTININLPIVDPVSAKQVAVLSKNCAEFGIRLHP
                     MGDPGQGIVHVIGPEFGLTQPGMSIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQT
                     LPQMRPKTMSIRVDGDLPAGSTAKDIILAIIGKIGTGGGIGSIVEYRGSTIQALSMEG
                     RMTICNMSIEAGAKAGMIAPDDITYAYVEGRPHAPKGAAWERALDAWRALPTDDNATF
                     DHEVTLDGTKIAPHVSWGTNPGQVISIDSSVPNPDQFDDPGAREAAARALAYMDLRAG
                     TPMRDIPVDTIFIGSCTNSRIEDLRLAAGVVEGRHVRNGMRAMVVPGSMRVKAQAEQE
                     GLDRIFLKAGFEWRAAGCSMCLGMNPDQLSPGERCASTSNRNFEGRQGKGGRTHLVSP
                     AVAAATAIVGTFATPADLG"
     gene            complement(2120..>2585)
                     /locus_tag="VFI12_01215"
     CDS             complement(2120..>2585)
                     /locus_tag="VFI12_01215"
                     /inference="COORDINATES: protein motif:HMM:NF020091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HET7055045.1"
                     /translation="LADRKKHVTAADLEALVSHQLETQGHAISLVQWKVDIASGEPAT
                     ATVVLAHNGQQLVGSAEGNGPVEAILRAVDAATGIDAELEYFNVEAIGPEKDAQGTVN
                     VRIRHDDELYTGRGVAPDIVEASARAYVAALSRLEQKQPSTVIAGSSASRWT"
BASE COUNT          423 a          830 c          841 g          491 t
ORIGIN      
        1 tgacatgctg aagcagggcg ggccaggcga gctcgaaccg atccaggttg tccggcaact
       61 cgacgacgga gacgcggatc accgggcctc accgccgatc gtcaccggca gaaagccagg
      121 acggtgcgcc tcgaatgctg cgatgtcgtc ctgatgctgg agcgtaaggc cgatgtcgtc
      181 gaggccattc agcagccggt gctgcgtgaa gtcgtccatc gggaactcga cctcgacgcc
      241 gatctccggg accgagagtg tccgatttgc gacgtcgatc gtcatctgca agcccggctc
      301 ttcacttgcc gccttgagca ggagatccgc gacgtcctgg tcgacttgaa ccgggatcag
      361 gccggtcttg gtcgagttgt ttcggaaaat gtcgccgaag cggggcgaga cgacggcttg
      421 gatgccgtag tccatcagcg cccagaccgc gtgctcgcgc gaggaaccag tgccgaagtt
      481 cggaccggca acgaggatct tcgcgttctg gaattccttt ttgttgagga cgaagtccgg
      541 gtcgctttgt cgccaggatg cgaagagtcc ctccccgaaa ccggtccgct cgacccgctt
      601 caagtacgaa gcggggatga tttggtcggt gtcgacgtca ctccggtcga gtggagcgac
      661 tatgccggta atcttccgaa ctgcctgcat gtcctacccc aagtccgccg gcgtcgcgaa
      721 cgtgccgaca atggccgtcg ccgcggccac ggccggagac acgaggtgcg tgcgaccgcc
      781 cttgccttgc cggccctcga aattccgatt actggtcgaa gcgcagcgct cgccgggcga
      841 gagctggtcc gggttcatac cgagacacat cgagcacccg gctgcgcgcc attcgaagcc
      901 ggcctttagg aagatgcggt ccagcccctc ctgctctgcc tgcgccttga ctcgcatcga
      961 tcccggtacg accatcgccc gcatcccgtt ccggacatgc cggccctcta cgacaccggc
     1021 ggcgaggcgc aagtcttcaa tccggctgtt cgtgcaggag ccgatgaaga tcgtgtcgac
     1081 cgggatgtcg cgcatgggtg tgccggcccg caaatccatg tacgcgagcg cccgggcggc
     1141 cgcctcgcgt gcaccgggat catcgaattg gtccgggttc ggcacggagc tatcgatcga
     1201 gatcacctgg cccggattcg tgccccacga gacgtgcggg gcgattttcg tgccgtcgag
     1261 cgtgacctcg tgatcgaagg tcgcattgtc gtcggtgggc aaggctcgcc aggcgtcgag
     1321 cgcccgctcc cacgccgcgc ccttcggcgc gtgcggccgt ccctcgacgt aggcgtatgt
     1381 aatatcgtcc ggcgcgatca tcccggcctt cgcgcctgcc tcgatcgaca tgttgcagat
     1441 cgtcatccgg ccctccatcg agagcgcctg gatggtcgag ccgcggtact cgacgatcga
     1501 gccgatgcca ccgccggtcc cgatcttgcc gatgatcgcg aggatgatgt ccttcgcggt
     1561 cgatccggcc ggaagatccc catcgacccg gatcgacatg gtcttcgggc gcatctgggg
     1621 cagggtctga gtggcgagga cgtgttcgac ctcgcttgtc ccgatcccga acgcaagcgc
     1681 gccgaatgcg ccgtgcgtcg aggtgtgcga gtcaccgcaa acgatcgaca tgcccggctg
     1741 ggtcagaccg aactcggggc cgatcacgtg cacgataccc tgtccagggt cgcccattgg
     1801 gtgcagccgg attccgaatt ccgcgcagtt cttcgaaagg accgcgacct gtttggccga
     1861 gactggatcg acaattggga gattgatgtt gatcgtgggc acattgtgat cggctgttgc
     1921 gatcgtcaaa tcgggtcgtc gaacgggtcg accggccatc cgcagcccat caaacgcctg
     1981 cggcgacgtc acttcgtgca ccagatgcag gtcgatgtag agcaaatccg gctcgccggg
     2041 cgcggagcgg acgacgtgct gctcccaaac cttgtcggcg agtgttcgcg gttgagtggt
     2101 cattctgtat ctctgtctct taggtccagc gcgatgccga tgatccggca atgacagtcg
     2161 aaggctgctt ctgctcgagg cgactcagcg cggcgacgta ggcccgcgcg ctcgcctcga
     2221 cgatgtcggg cgcaactcca cgtccggtgt agagttcgtc gtcgtggcgg atgcggacat
     2281 tgaccgtgcc ctgggcgtcc ttctcggggc cgatcgcctc gacgttgaaa tattcgagct
     2341 cggcgtcgat cccggtggcg gcatcgaccg cgcgcaggat cgcctcgaca ggcccgttac
     2401 cctcggcact gccgacaagc tgttggccgt tgtgagccag cacgaccgtg gcggtcgccg
     2461 gttcgccgct cgcgatatcg accttccact gcacgaggga gatcgcgtgg ccctgcgtct
     2521 cgagctggtg gctgaccagc gcctcgaggt ccgccgctgt cacgtgcttc ttgcggtcgg
     2581 ccagg
//
LOCUS       DASSGX010000072         4147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1209337, whole genome shotgun sequence.
ACCESSION   DASSGX010000072 DASSGX010000000
VERSION     DASSGX010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1209337"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VFI12_01220"
     CDS             <1..432
                     /locus_tag="VFI12_01220"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HET7055046.1"
                     /translation="DAAFSNAIYATIAPFEPDLIVCGGFMKRLVVSPDWEGRILNIHP
                     CLIPESNAAGAGFYGGRVHEAVIACGATMSGATVHVVDNEYDHGPVVMKETVPVLPGD
                     TATDLAARVFALECDLYPRAIAAYLSQHPGLIRRSRVDDRR"
     gene            511..1599
                     /gene="apbC"
                     /locus_tag="VFI12_01225"
     CDS             511..1599
                     /gene="apbC"
                     /locus_tag="VFI12_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HET7055047.1"
                     /translation="MPATNLTKEQILDLLRPIEDPSLNQSIVDLGMVKTAESTGNGIT
                     IKLEQLTSLQPHKEQIEAAIREALADVGEPTITWSFRVRASGQGRSDAQAIKGVKNVI
                     AIASGKGGVGKSTVAVNLAVAMAKTGASVGLLDTDVYGPSIPIMMGTSEKPLMRNNKM
                     VPIEAHGVKLMSIGFILEPDKALIWRGPLVAQLITQFLNDVDWGDLDYLVLDLPPGTG
                     DVQLTLVQRIPISGAVIVTTPQEVAMADARKGLRMFEEVKSPVLGIIENMSYFQCPDC
                     GSRHAIFGEGGGQRLAEEGDVELLGQIPIETIVREGSDTGMPVVASDPDSLTGTAFAR
                     AAERVAMKLAEDAVAKPRKPVLMFKSVR"
     gene            1596..2582
                     /locus_tag="VFI12_01230"
     CDS             1596..2582
                     /locus_tag="VFI12_01230"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7055048.1"
                     /translation="MSEKVIPVSREFSGETDLARLVAMLHAVATADKDGMTVSPDDYR
                     AEWVGDEPGWVRSLRVWEAGDRFVAAFGNWHQPEDTLDRAYGELDVHPDWREPVFVDE
                     VVAASIDAVSELADRPVEHRLNAFLSQEWKQAALERAGYAAERYFFRMSAPITGAIRN
                     PQIAEGFSIRPMAGDAEVDGWVAAFNGAFAGHYDPPTHTAAEKRNSMTAAGYLPEADL
                     VLIDGDNRIIGVGRNSREILEGGQDKGWINSVAILPEYRGKGLGRALLLSSMAALQEA
                     GFTRAHLHADSENESGALQLYESAGFTVDSRIAVYMRMVIPADNLQQIPAIP"
     gene            complement(2691..3158)
                     /locus_tag="VFI12_01235"
     CDS             complement(2691..3158)
                     /locus_tag="VFI12_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055049.1"
                     /translation="MLGGTSLADHSLVGAWLLTLPTGIALSYFGADGTALISWPACEQ
                     GADGLVRYTTPGLGTWERVDERKGYVNVVQVLSDAEGHFLGSRSLHCLPVVSEDGESF
                     EDDGRAIRVYVRDGANALATILGKDGDSAPMSGVRMRPGSPGFPGQSGSIAEQ"
     gene            complement(3460..>4147)
                     /locus_tag="VFI12_01240"
     CDS             complement(3460..>4147)
                     /locus_tag="VFI12_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET7055050.1"
                     /translation="WRRERGNVTNITVPLNALGLVVCDRGDHRAAVALLAEGLTRWEQ
                     DGGGNREVLAEWLAAVARLAACRGRPGTAGRLYGAAEALFDAIGVLLVVPPRSLYLRH
                     VDALRSTLGTEAFAATWAAGRALPLEQAIEEARAVTVDHVAAGAAPPAGAPDVNPALT
                     PREQEVLRLLARWMTDREIADALFISHRTVNAHVASILAKLGVASRREAAMRSREFGL
                     ASDSPGNPGA"
BASE COUNT          725 a         1353 c         1389 g          680 t
ORIGIN      
        1 gatgccgcgt tcagcaacgc gatctacgcc actatcgcgc cgttcgagcc ggacctgatc
       61 gtctgcggcg ggttcatgaa gcggctggtc gtgtctccgg actgggaggg acggatcctt
      121 aacattcatc cgtgcctgat cccggagtcg aacgcggccg gcgccgggtt ctacggcggt
      181 cgcgtgcatg aagcggtgat cgcgtgcggc gcgacaatgt ccggggcgac ggtccacgtc
      241 gtcgacaacg agtacgacca cgggccagtc gtgatgaagg agacggtgcc ggtgctgccc
      301 ggcgataccg cgaccgatct tgccgcccgg gtcttcgcac tcgagtgcga cctctatccc
      361 cgggcgattg cggcatatct atcgcagcat cccggcctga tccggcgctc ccgggttgat
      421 gatcggcggt aaacttacca cgacagtcag gattgtcacg ggcattccgc ccgtctccag
      481 ataccatcac actcgcgagg aaacgccttc atgcccgcca ctaatctcac caaggagcag
      541 atcctcgatc tgctgcgccc aatcgaggac ccatcgctga accagagcat cgtcgacctt
      601 ggcatggtca agacggctga atcgaccggc aacggcatca cgatcaagct tgagcagttg
      661 acctcactcc agccgcacaa ggagcagatc gaagccgcga tccgcgaggc gctggctgac
      721 gtgggtgagc cgacgatcac ctggagcttc cgcgtccgag ccagcggtca gggccgctcc
      781 gacgcgcagg cgatcaaggg cgtcaagaac gtgatcgcga ttgcttcggg caagggcggc
      841 gtcggcaagt cgaccgtcgc ggtcaacctg gcggttgcaa tggccaagac cggcgcatcg
      901 gtcggcctgc tcgataccga tgtttacggg ccgagcatcc cgatcatgat gggcacctcc
      961 gagaagccgc tgatgcggaa caacaagatg gtgcccatcg aagcgcatgg cgtgaagctg
     1021 atgtcgatcg gcttcatcct cgagccggac aaggcgctga tctggcgcgg tccgctcgtg
     1081 gcgcagctca tcacgcagtt cctgaacgac gtcgactggg gcgatctcga ctacctggtc
     1141 ctcgacctgc cgccgggcac gggcgatgtc cagctcaccc tcgtccagcg gatcccgatc
     1201 tcgggcgcgg tgatcgtgac gacgccgcag gaggtcgcga tggccgacgc ccgcaagggt
     1261 ctgcggatgt tcgaagaggt gaagtcgccg gtcctgggca tcatcgagaa catgagctac
     1321 ttccagtgcc cggattgcgg cagccggcac gctattttcg gcgagggcgg cggtcagcgg
     1381 ttggccgagg agggcgacgt cgagttgctc ggccagatcc cgatcgagac gatcgtgcgg
     1441 gagggcagcg ataccggcat gccggtcgtc gccagcgatc cagactccct gaccggcacg
     1501 gccttcgccc gcgcggctga gcgggtggca atgaagctgg cggaggacgc ggtcgcgaag
     1561 ccgcgtaaac cggtcctgat gttcaagtcg gtccgatgag cgagaaggtc atcccggtat
     1621 cccgcgaatt tagtggcgag accgacctcg cgcggttagt cgcaatgctg cacgccgtcg
     1681 caaccgcgga caaggacggc atgaccgtat ccccggacga ctatcgcgcg gagtgggtcg
     1741 gtgacgagcc gggttgggta cggagccttc gggtctggga ggccggcgat cggttcgtgg
     1801 ccgcgttcgg caattggcac cagccggagg acacgttgga tcgcgcctac ggcgaactcg
     1861 atgtccaccc cgattggagg gagccggtct tcgtcgacga agtcgtcgcg gcgtcgatcg
     1921 atgcggtctc cgagctggcc gaccggccgg tggagcaccg gctcaatgcg tttttgagcc
     1981 aggagtggaa gcaggcagcg ctcgaacggg ccggctacgc agccgagcgt tactttttcc
     2041 ggatgagcgc gccgattacc ggagccatcc ggaacccgca gatcgccgag ggcttctcaa
     2101 ttcggccgat ggccggcgat gcggaagtcg atggctgggt cgcggcgttc aatggcgcct
     2161 ttgccggcca ctatgacccg ccgacccaca cggcggcgga gaagcgaaac tcgatgactg
     2221 ccgccgggta tctgccggag gccgatcttg ttctgatcga cggcgacaat cggatcatcg
     2281 gtgtcggtcg caactcgcgg gagatcctcg agggcgggca ggacaaggga tggatcaact
     2341 cggtcgcgat cctgcccgaa taccggggca agggactcgg ccgcgcgctg ctgttgtcgt
     2401 cgatggccgc tctccaagag gccggcttca cgcgagcgca cttgcacgcc gactcggaga
     2461 acgagagcgg cgcgctccag ctctacgagt ctgccggctt caccgtcgat agccgcatcg
     2521 ctgtttacat gcggatggtc atccccgccg acaacctcca gcaaattccc gcaatcccat
     2581 agtaagcagc gcgcttccaa gcgagaaagt ggcgattcct aggtcacacc gaagcggttc
     2641 ctgcctcgcc aggaaccgct tcgcgtctcg tgtagacgat gaagtgcgcg tcactgttct
     2701 gcgatcgaac cggactgccc cggaaatccg ggtgatcccg gccgcatgcg tactcccgac
     2761 atcggggccg agtctccgtc cttgccaagg atggtggcaa gggcgttggc gccgtcgcgg
     2821 acgtagaccc ggattgcccg accgtcgtcc tcgaacgact ccccgtcttc gctgacaaca
     2881 ggaagacagt gcagcgagcg ggaaccgagg aaatggccct ccgcatccga gaggacctgg
     2941 acgacgttga cgtatccctt gcgctcgtcc acgcgttccc aggtgccgag ccccggggtc
     3001 gtgtaccgaa ccaagccgtc tgccccctgc tcgcaggctg gccaggagat cagagcagtt
     3061 ccgtccgcac cgaagtacga gagtgcgatc ccggttggga gagtcaggag ccaggcgcca
     3121 accagggaat gatcggcaag gctcgtgccg cccaacatgg ccccccgggc cggtcctgcc
     3181 agagccgacc cgacgggagc tgcaaggccc aggctgagcc cacttgcccc caaacctgaa
     3241 atcgcaacgc gacgagatac cggtgctggc gatcgctgcg tgaacatgtc ggcgtctcct
     3301 tgccgatcgt cgccgcggtc actggccccg cctgggttcg tccgtggcga tggagccgat
     3361 cctgcggcca ggcagtgggg ctccgcatct gcagccggca cctatttggg aagggttttg
     3421 gacggccgaa tcagtattcg ctacctacgc tcgaactcct cacgcaccag ggttccccgg
     3481 tgagtctgac gcaagtccga actctcggct gcgcatggcc gcctcgcgcc gggacgctac
     3541 gcccagcttc gccaggatgc tcgccacgtg ggcgttgacc gtgcggtggc tgatgaagag
     3601 agcgtcggcg atctcgcggt cggtcatcca ccgcgccaac aggcgcagca cttcctgctc
     3661 gcgaggggtc agagccggat tgacgtctgg cgcaccggct gggggcgcgg ccccggcagc
     3721 gacgtgatcg accgtcactg cccgcgcttc ttcgatggcc tgctccaggg gcagcgctcg
     3781 gcctgcggcc caggttgcgg caaaagcctc agtgcccaag gtgctccgga gggcgtcgac
     3841 gtgccgaagg tagagggagc ggggaggcac gaccagcagg actccgatcg cgtcgaacag
     3901 cgcctccgcc gcgccgtaca ggcggccggc ggtccccgga cgcccgcgac aagccgccag
     3961 ccgggccacg gcggcgagcc actcggccag gacttcccgg ttcccgccgc catcctgctc
     4021 ccaccgggtc aggccctctg ccagcagggc gaccgctgct cggtggtccc cgcggtcgca
     4081 gacgacgagg ccgagggcgt tgaggggaac ggtgatgttt gtgacgttgc cgcgctcccg
     4141 ccgccag
//
LOCUS       DASSGX010000073         2096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_557582, whole genome shotgun sequence.
ACCESSION   DASSGX010000073 DASSGX010000000
VERSION     DASSGX010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557582"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VFI12_01245"
     CDS             complement(<1..590)
                     /locus_tag="VFI12_01245"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7055051.1"
                     /translation="MRPFLAMVKANLKMTVRNRQALFWNLAFPAIFILIFGAVFNNDS
                     GVNYDVGISGADSQYRQSVVQALDSIDGIKVHVGDETKELKALKDNDRDYVLVFSEPV
                     AGQLGAPVQISYDQTQGPSAQVGINVLSQVLMSVAGGENAVPITTQPIETLNIKYIDF
                     FIPGILGMALMNSGIIGLSTAFVTYREKGILRRIKVT"
     gene            complement(602..1612)
                     /locus_tag="VFI12_01250"
     CDS             complement(602..1612)
                     /locus_tag="VFI12_01250"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7055052.1"
                     /translation="MTSARQPMIDIHDLVKRYGDFTAVDGIDLSVAYGEIFGILGPNG
                     AGKTTTLEMIEGLREPDRGSITVDGIDAVRESDRVRPIIGVQLQTTALFEYLNASELI
                     ELFAGLYESEASAGRIDELLGMVDLREKAKSQVNTLSGGQRQRLSIALALVNQPKVVF
                     LDEPTTGLDPGARRDLWKMIQGIRAGGMTVVLTTHYMEEAEVLCDRVAVMDRGRVIAC
                     DTPGALIASLGIEATVRARITGGSLDLPDLDALPAVLGASIEGDRIALQTRDVQSTLI
                     ALLRLVEARGVTLAEMTSQQSTLEDVFLSLTGRTYDDTSNEQAEAAAPAAAGGRRRRR
                     GL"
     gene            1760..>2096
                     /locus_tag="VFI12_01255"
     CDS             1760..>2096
                     /locus_tag="VFI12_01255"
                     /inference="COORDINATES: protein motif:HMM:NF019236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HET7055053.1"
                     /translation="MRLAASHHNRYRAGLAVLLLLVLALPLSPLAPREVAAAGTYYVA
                     TTGNDATGTGTLANPWRTIQKAADSIGPGSTVLIQPGTYAEAAEIAITATAASPVIFR
                     GNGAGVVIDA"
BASE COUNT          352 a          728 c          639 g          377 t
ORIGIN      
        1 gtcaccttga tccgccgcag gatccccttc tcccggtacg tcacgaacgc ggtcgaaagg
       61 ccgatgatcc ccgagttcat cagcgccatg ccgaggatgc ccgggatgaa gaagtcgatg
      121 tacttgatgt tgagcgtctc gatcggctgg gtcgtgatcg gcacagcgtt ctcgccgccc
      181 gccacgctca tcagcacctg cgagagtacg ttgatcccga cttgcgcgct cggcccctgg
      241 gtctggtcgt acgaaatctg gaccggcgcg ccgagctgac ccgccaccgg ctcgctgaag
      301 acgaggacat agtcgcggtc gttgtccttg agcgccttca actctttcgt ctcatcaccg
      361 acatggacct tgatcccgtc gatcgaatcg agcgcctgga caaccgactg ccggtactgg
      421 gaatcggcgc cgctgatccc gacgtcgtag ttgaccccgg aatcgttgtt gaacaccgcc
      481 ccgaagatca ggatgaagat ggccgggaag gcgaggttcc agaagagggc ctggcggttc
      541 cggaccgtca tcttcaggtt cgccttgacc atggcaagaa acgggcgcat gaaattcctt
      601 cctataagcc gcgtcgccgg cgccgcccgc ccgcggcggc aggcgcggct gcctcggcct
      661 gttcattact ggtgtcgtcg tatgtcctgc ccgtcaggct gaggaagaca tcctcgagcg
      721 tcgattgctg gctcgtcatc tcggccagcg tcacgccccg cgcctcgacc agccgcaaca
      781 acgcgatcag cgtcgattgc acatcccgcg tctgcagcgc gatccggtcg ccttcgatcg
      841 atgcgccgag caccgccggc agcgcgtcga ggtcgggcag gtccagcgac ccgccggtga
      901 tccgtgcccg caccgtcgcc tcgatgccga gtgacgcgat cagcgcgccg ggcgtgtcgc
      961 aggcgatcac gcggccgcgg tccatgaccg cgacccgatc gcagagcact tccgcctctt
     1021 ccatgtagtg cgtcgtcagc acgacggtca tcccgcccgc ccgaatcccc tggatcatct
     1081 tccagaggtc acggcgcgcc cccggatcga gcccggtcgt cggctcgtcg aggaagacca
     1141 ctttcggctg gttaaccagc gccagcgcga tcgagagccg ttgccgctgc ccgcccgaga
     1201 gcgtattgac ctgcgacttc gccttctcgc ggagatcgac catcccgagc agctcgtcga
     1261 ttcgccccgc cgacgcctcg ctttcgtaga ggccggcgaa gagctcgatc agctcggaag
     1321 cgttcaggta ctcgaagagc gccgtcgtct ggagctggac gccgatgatc ggccggaccc
     1381 ggtcgctctc acggacggca tcgatcccgt cgaccgtaat cgagccgcgg tccggctcgc
     1441 gcaacccctc gatcatctcg agcgtggtcg tcttgccggc gccgttcggg ccgaggatgc
     1501 cgaagatctc gccgtacgcg acggagagat cgatcccgtc gacggccgtg aaatcgccgt
     1561 accgcttgac gaggtcgtgg atgtcgatca tcggttgtcg ggctgaagtc aacgtgtgtc
     1621 tcccattatg gccgttcaat gctactaccg atccatctcc ggacaggacc caagcggcac
     1681 taccgtcctg tccaatcccg gccgatttcc ggcagaactg cactgccggg cttggctcga
     1741 tcccggccgg aggcattgcg tgcgattagc tgcatcacat cacaacaggt accgcgccgg
     1801 gctggctgtg ctcctgctcc tcgtgctggc gctaccgctc tcgccgcttg ccccgcgcga
     1861 ggtcgcagcg gccggtacgt attacgtcgc cacaaccggg aacgatgcaa ccggaaccgg
     1921 cacgctcgcc aatccatggc gcacgattca gaaagccgcc gattccatcg gacccggttc
     1981 gacggtcctg atccagcctg gaacctacgc ggaagcagcc gagatcgcga tcaccgccac
     2041 cgccgccagc ccggtcatct tccggggcaa cggcgcgggg gttgtgatcg atgccg
//
LOCUS       DASSGX010000074         3284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_13757, whole genome shotgun sequence.
ACCESSION   DASSGX010000074 DASSGX010000000
VERSION     DASSGX010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13757"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            313..612
                     /locus_tag="VFI12_01260"
     CDS             313..612
                     /locus_tag="VFI12_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055054.1"
                     /translation="MAFRKQPQAAADDEFVAESISPGCPMLIRAAAHHPTRPNALLMR
                     CALGWSLHDELDYERCGATGAVQDCWRVHPERTPVVALPGSEDALDVKSRQHAAD"
     gene            725..2101
                     /locus_tag="VFI12_01265"
     CDS             725..2101
                     /locus_tag="VFI12_01265"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HET7055055.1"
                     /translation="MRRLVTVGFAWLIALVLVATPMMTAPLSSVSAQTGVGTPIADDP
                     PADETPVDGPSEEASPTPEVTEPVVEPPATDVPSDTPDLEGTPIEASPVDEPTEGATA
                     TPTATTTPDFTSAAVLSDITITVKCATSPETVRVTNNGADVIQLTSFSTFIDLTADEP
                     FALNRNLKPGTTAIFQSGQGAQYGTVLTTSYLFTNSAYDAEGVKIGTSVGEAVQMCDP
                     KPLPPPPPVGKLSDLKVTLSCTTDPETIRVTNNGAGWITLKGIATYIDPIADEPFAIM
                     PRRVLKPGQTAIYQSGPGAKYGTILTTQSIFTNAAYEKDGVRISTDVGKLYKACPAKP
                     VPPERWIEVNLSAQYLTAWVGNTKVNGTYVSTGKPGFETPTGTFRILYRYWTQTMAGC
                     IQGECYYVPDVPWVQYFTNYGHALHGAYWHNDFGTTRSHGCVNLPLPFAEWLWYWATY
                     GTRVWIHY"
     gene            complement(2243..3115)
                     /locus_tag="VFI12_01270"
     CDS             complement(2243..3115)
                     /locus_tag="VFI12_01270"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET7055056.1"
                     /translation="MDDGQANRLLGYPPDARLLIINADDYGMCHAVNDAVLRTIRDGV
                     VTSTSLMTPCPWAPHAMHLLRDHPQLAFGVHLTLVSEFSPYRWGPVASRDRVPSLVDE
                     AGHLVIYDHIPDLLERARLDEVEIEFRAQIDVVLAAGLLPTHLDWHCIADGGRDDIFE
                     LTLRLAREFGLAMRIHDPAHAARCRTLGLPANDHGVLDSYHLDPAEKPVTFVELLRRL
                     PAGLSEWAAHPSLGDTEAQAIEPETWQIRRADLDFFTSQTARDVIAEEGITLLNYRAL
                     QPYWSARIDNNAQT"
BASE COUNT          625 a         1043 c         1049 g          567 t
ORIGIN      
        1 gatcgcctca cgtccaccct gcggcggctc gcaagccggg actaggggac cggcgacgaa
       61 gtgcgctgca ctcccctata ctccggagag ggcgtcgcat tggggcgacc ccgtgtaaac
      121 ccgctggttg gcacgctgca ccgcaatttg ttcggccact ccatcgcgga gtacctgtcg
      181 aagaccactg caattgccgt cctgaatcgc tttcgacctg ggaacgaagc cgtatctcgc
      241 tcgatccgca ctgcttgatg gaattggtcc cgcgccgcgc agcgtgccat tgcccgggga
      301 aaggcaagcg caatggcatt tcgcaagcaa ccacaggctg cggcggacga cgagttcgtc
      361 gcggaatcaa tcagtcccgg ctgtcccatg ctgatccggg cggcggccca tcatccgacc
      421 agaccgaacg cgctcctgat gcggtgcgcg cttggttggt cgctccatga tgagctggac
      481 tacgagcggt gcggcgcaac cggcgctgtg caggactgct ggcgcgtgca tcccgagcgc
      541 accccggtcg tcgcccttcc gggctcggag gacgccctcg acgtgaagtc caggcaacac
      601 gccgccgact gaacctattg acctcgttaa ccatggtcac taggatgaaa caaattcccg
      661 cgtagacgcg tattaatatc gacaggaatt gggaagcgct tcggttgggt ttggggaggg
      721 ttcgatgcgt cgtctggtca ctgtgggctt tgcatggctc atagcactgg ttcttgttgc
      781 cactccgatg atgacggcgc cgctttccag cgtctccgcc cagaccgggg tcggcactcc
      841 gatcgcggat gatccaccgg cggacgagac gccggtcgac ggtccttctg aggaagcttc
      901 accgacaccc gaggtcaccg aaccggttgt tgagccgccg gcgaccgacg taccgagcga
      961 tacaccggac ctcgaaggca ccccaatcga ggcatcgccg gtcgacgagc cgaccgaggg
     1021 ggccacggcc actccgacgg caactacgac gcccgacttc acgagtgcgg cggtcctctc
     1081 agacatcacg atcacagtca agtgcgccac gtctccggaa accgtccggg tcacgaataa
     1141 cggcgcggat gtcatccaac tgacgagctt cagcacgttc atcgacctga ccgccgacga
     1201 gccatttgcg ctcaaccgga acttgaagcc cggcacgacg gcaatcttcc agtctggtca
     1261 gggcgcgcag tacggcactg tcctgaccac gagctatcta ttcacgaaca gcgcctatga
     1321 cgccgaagga gtcaagatcg ggacgtcggt cggcgaggct gtccagatgt gcgatccgaa
     1381 gccgctcccg cccccgccgc cggttggcaa gctgagcgac ctcaaggtca cgctgagttg
     1441 cacgacagac ccggagacga tccgcgtcac caacaacggc gccggctgga tcacgctcaa
     1501 gggcatcgcg acctacatcg acccgatcgc cgacgagccg ttcgcgatca tgccgcggcg
     1561 cgtcctgaag cccggccaga cggccatcta ccagtcgggc ccgggggcca agtacggcac
     1621 gattctgacg acccagtcaa tcttcacgaa cgcggcctat gagaaggatg gcgtgcggat
     1681 ctcgacggac gtcggcaagc tctacaaggc ctgcccggcg aagccggtcc cgccggaacg
     1741 gtggatcgag gtcaacctga gcgcgcagta cctgacggcg tgggttggca atacaaaggt
     1801 caacggcacc tacgtgagca ccggcaagcc gggcttcgag acaccgaccg gcacgttccg
     1861 tatcctttac cggtactgga cgcagacgat ggccggctgc atccagggcg agtgctacta
     1921 cgtgccggat gtgccgtggg tccagtactt tacgaactac ggccacgcac tgcacggggc
     1981 gtactggcac aacgacttcg gcacgacacg aagtcacggg tgcgtaaact tgccgctgcc
     2041 ctttgccgaa tggctctggt actgggccac gtacggcacc agagtctgga tccattacta
     2101 ggtcgttggt agatggcggt tggtcgatgg agttgatccg ccggggccat tgacccgcca
     2161 ccaacatcgc gtaaactcgc acgattgggc cgggagaaac ccccggccca atcgtattgg
     2221 ttgggtcaga tggcatctac ccctacgttt gcgcgttgtt gtcgattcgg gcggaccagt
     2281 agggttggag ggcgcgatag ttcagcaggg taatgccttc ctcggcgatg acgtcccgcg
     2341 cggtctggga cgtgaagaaa tcgaggtccg cccgtcggat ttgccaggtc tcgggctcga
     2401 tcgcctgcgc ctctgtgtca ccgaggctgg gatgggccgc ccactcgctg agcccggccg
     2461 gcaaacgccg taacagctca acaaacgtca ccggcttctc cgctgggtcg aggtggtagc
     2521 tatccagcac gccgtggtca ttcgccggga gaccgagggt tcggcatcgc gcggcgtggg
     2581 ccggatcgtg gatccgcatc gccagaccga actcccgcgc cagccgcagc gtcagctcga
     2641 aaatgtcgtc ccggccaccg tcggcgatac agtgccagtc gagatgcgtc ggcaaaagtc
     2701 cggcagccag gaccacatcg atttgggccc ggaactcgat ctcgacctcg tccagccggg
     2761 cacgctccag caaatccggt atgtggtcgt agatgacgag gtggcccgcc tcgtcgacca
     2821 gcgacggcac gcggtcccgg gaggcgactg gcccccaccg gtatggtgaa aattcggaaa
     2881 cgagcgtgag gtgaacgccg aatgccagct gcgggtgatc gcgcagcaag tgcatcgcgt
     2941 gcggagccca gggacagggc gtcatcagcg aggtggacgt aacgacgccg tcccgtatcg
     3001 tgcgcagaac agcatcgttc accgcgtggc acatgccgta gtcgtcggca ttgatgatca
     3061 gcaggcgggc atccggtgga tagccgagca gccgattcgc ctgaccgtcg tccatcggtt
     3121 tcactgtggg atcagaagcg acgggcggcc ttttgggcct cggcttcgga gcggggcgcg
     3181 tgttccttga tgtactgctc gacctcggcg ggtgtgaact gccgcagatc catcgcggcg
     3241 ttcatcggcc gctccgggaa gtagaggaag aaggtgaagc ggat
//
LOCUS       DASSGX010000075         3615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1210371, whole genome shotgun sequence.
ACCESSION   DASSGX010000075 DASSGX010000000
VERSION     DASSGX010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1210371"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VFI12_01275"
     CDS             <1..360
                     /locus_tag="VFI12_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055057.1"
                     /translation="VVDRVHAALTPRDERISVDVVVLGKRPAGQVAVTSRIVVAAAEI
                     LRSLSHSPAADASSTDANAAIAMGLPAVCIGLTTGGNAHRLDEYIDLAPVKTGLAQLI
                     LLTLAVSEDLARGRLSP"
     gene            363..3110
                     /locus_tag="VFI12_01280"
     CDS             363..3110
                     /locus_tag="VFI12_01280"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HET7055058.1"
                     /translation="MILSSDDALYPAGIWAVVGSDTDRPEGVLRIVLFDGAIPVEDRS
                     GTPAEVLDFIDMHCGLGLEFAAVSSGSWLARYVGDDAALDLSAFVALDIWSGVIGIRP
                     RLVASTCAAVADRMCEVLARLAALDPGVLLRSDAYLGIRRNGEQRLIRSALAHSQAMH
                     GPASAKPPELAFLTNREKPERLKRIKGADRVEPAEIAASFDIGGPISKRFDAWEPRGQ
                     QREMAVAVAEELRDGGELIAEAGTGTGKSLAYLVPALTNAVLTGEQVVVATNTRVLQD
                     QLAGKDAPLAMAAVREAVPGAEPRVQVLKGRANYLCLRRWFAEMQQKPMPATDEEASF
                     RARANIWLNLTESGEQADLPMERGHAAIFNRVSAEGDACDASRCQFQQRNQCFLYRAR
                     RAADAAHVVITNHALLLTDVVQEGEALPEARHLIIDEAHHLEDQATNSFEVAVSARLI
                     NQVLNAISGDARRGQPGLLVEIGALAHNRQLLSLSSDQLDRVLKAIESIETAIDVVRR
                     ESASFFAELANVVEHAGERSRDYSTRLRVTAALRHGSVWETVERRWDALNIALATAVS
                     HIAILQALIPDVAASAARQDVDLEQLGVRVEDVENRLGVFRYELTEIGMNVNDAIHNP
                     NADQVYWLEMRQGERRDTTLHSAPLQLGDYLRRHLYSRLDSLVLTSATMTTGGDSRFI
                     RRRLGLEDADEITLSSPFDYESNAMILIPTDLPEPNQPGFDLAAHEAIYATAVAAGGR
                     TLVLFTSIYAMNQARAALGDRLRDAGLQLVTQHEDGSAEQLAERLRSFDRTVVFGAGA
                     FWEGVDVPGPALSALVIAKLPFPVPTDPIHAARSETFDNGWTEYTMPQTILKLRQGFG
                     RLIRTQTDRGVCLILDRRLISKRYGAQIVNALPNATREYGTISQIGPTVARFLNDGVA
                     P"
     gene            3107..>3615
                     /locus_tag="VFI12_01285"
     CDS             3107..>3615
                     /locus_tag="VFI12_01285"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
                     /protein_id="HET7055059.1"
                     /translation="MTASTVDRNLALELARVTEAAAIMAARWMGRGDKNAADQAAVDA
                     MRSVLNSLQMDGIVVIGEGAKDEAPMLFVGEEVGAGGAPHVDIAVDPIDGTRLLSLGM
                     PNAIAVVAVAERGTMFASPDISYMNKIAVGAEAAGFVDIKAPVQENLRRIAAAKGVTI
                     GDLTVVVLDR"
BASE COUNT          621 a         1165 c         1219 g          610 t
ORIGIN      
        1 gtggtcgacc gggtacacgc tgccctgacg ccgcgagacg aacgaatctc ggtcgacgtt
       61 gtggtgctcg gcaagcggcc ggccgggcaa gttgccgtga ccagccggat cgttgtcgcg
      121 gcggccgaaa tcctgcgctc gctgtcgcat tcgcccgcgg ccgacgcctc atccaccgat
      181 gccaatgcgg cgatcgccat gggcctgccg gccgtttgca tcggcttgac gaccggcggt
      241 aatgcgcacc gcctcgacga gtacatcgac ctcgccccgg tcaagaccgg cctcgcccag
      301 ctcatcttgc tcacgctggc cgtctcggaa gatctggccc ggggacgcct ctcgccataa
      361 gcatgatcct ctcatcggat gacgcgctgt atcccgcagg aatctgggcc gtcgtcggca
      421 gcgacacgga ccggcccgaa ggcgtgctcc gaatcgtgct gttcgatggc gcaatcccgg
      481 tcgaagaccg atccggcact ccggcggaag ttctcgactt catcgacatg cattgcgggc
      541 tcggcctcga gttcgcagcc gtgtcgagcg gctcgtggct ggcgcgctac gtcggggacg
      601 atgcggcgct cgacctgtcg gcgttcgtcg cgctcgatat ctggtcgggc gtcattggga
      661 tccgaccccg gctcgtcgcg tcgacctgcg cggcggtggc ggaccggatg tgcgaggtgc
      721 tggcgcgact cgcggcgctc gatcccggcg tgctactccg atccgatgcc tatctcggca
      781 ttcggcggaa cggcgagcaa cggctgatcc ggagtgccct cgcccatagc caggcgatgc
      841 acggtccggc atccgccaag ccacccgagc tcgccttcct gaccaatcgc gagaagccgg
      901 agcggctcaa gcggataaag ggcgctgatc gggtcgaacc ggccgagatt gccgcgtcgt
      961 ttgatatcgg cggtccgatc tcgaagcggt tcgatgcctg ggagccccgc ggccagcagc
     1021 gcgagatggc ggtcgcggtc gccgaggaac tgcgggacgg cggcgagctg attgctgagg
     1081 cgggaaccgg caccggcaag tcgctcgcct acctggtccc ggcgctcacg aacgcggtcc
     1141 tgaccggcga gcaggtcgtc gttgcgacga acactcgcgt gttacaggat caattggccg
     1201 gcaaggatgc accactcgcg atggcagcgg tgcgggaggc ggttccaggg gccgagccgc
     1261 gcgtccaggt gctgaagggc cgcgcgaact acctctgtct gcgccgctgg tttgccgaaa
     1321 tgcagcagaa gccgatgccg gcgaccgacg aggaggcgtc attccgggcg agagccaaca
     1381 tttggctgaa cctcaccgag tcaggcgagc aggcagacct gccgatggag cgcgggcacg
     1441 cagcgatctt caaccgggtg tccgccgagg gcgatgcctg cgacgccagc cggtgccagt
     1501 tccagcaacg gaaccagtgc ttcctgtacc gggcacgccg agccgccgat gccgcacacg
     1561 ttgtcataac gaatcatgcc ttgctgctga ccgacgtggt gcaggaaggg gaggcgctcc
     1621 ccgaagcgcg gcacctgatc atcgatgagg cgcatcatct cgaggaccag gcgacgaaca
     1681 gtttcgaggt cgcggtctcg gcacggctca tcaaccaggt gctgaacgcg atctcgggcg
     1741 atgcgcgacg gggacagccg ggcctgctcg tcgaaatcgg cgcactcgcg cacaaccggc
     1801 aactcctgtc gctctcgagt gatcagcttg atcgcgtcct gaaggcgatc gagtcgattg
     1861 aaacagcgat cgacgtggtc cgccgcgaat cggcgtcatt cttcgctgaa cttgccaacg
     1921 tggtcgagca cgccggagag cggtcgcgcg attactccac gcggcttcgc gtgactgcag
     1981 ccctgcgcca cggttcggtc tgggagacgg tcgagcggcg ttgggatgcg ctcaacattg
     2041 cgcttgcgac cgccgtctcg cacattgcga tcctgcaggc gctcataccg gatgttgcgg
     2101 cgtcagccgc gcggcaggac gtcgacctcg agcagctcgg tgtgcgcgtc gaggatgtcg
     2161 agaaccgcct cggcgtcttc cggtacgagc tgacagagat cggaatgaac gtcaatgacg
     2221 cgatccacaa cccgaatgcc gatcaggttt actggctgga aatgcgtcag ggcgaacgcc
     2281 gggacacgac cctgcattcc gcgcccttgc agctcggcga ttatctgcgc cggcatctct
     2341 actcccgact cgattcgttg gtcctgacga gcgcgacgat gacgaccggc ggcgattccc
     2401 gcttcatccg gcggcgcctt ggccttgagg acgccgacga gatcacgctt tcctcgccgt
     2461 tcgactacga gtcgaatgcc atgatcctga taccgaccga cctgccggag ccgaaccagc
     2521 cagggttcga cctggcggcg cacgaagcga tctatgccac agccgtcgcg gcaggtgggc
     2581 gtacgcttgt cctgttcact tcgatctacg cgatgaacca ggcgcgggcc gcgctgggtg
     2641 accgcctgag agacgctgga ctgcaactcg tcacgcagca tgaggatgga tcggctgagc
     2701 aattggccga gcggttgcgc tccttcgatc ggaccgtcgt gttcggtgcg ggggcgttct
     2761 gggagggcgt ggatgtgccc ggcccggccc tgagtgcgct tgtgatcgcc aagttgccgt
     2821 tcccggtccc gaccgacccg atccatgcgg cgaggtcgga gaccttcgac aacggctgga
     2881 ctgaatacac catgccgcag acgatcctca agctgcggca gggcttcggc cgcctgatcc
     2941 gcacccagac ggaccgcggc gtctgcctga tcctggaccg gcggcttatc tccaaacgat
     3001 atggggcgca aatcgtgaat gcactgccta acgcgacaag ggaatacggc acaatctcgc
     3061 aaatcggtcc gacggtcgca cgatttctga atgacggagt ggcgccatga cagccagcac
     3121 ggtcgatcgt aacctcgcgc tggaacttgc ccgggtgacc gaggcggcgg cgatcatggc
     3181 cgcccgctgg atgggccggg gcgacaagaa cgcggccgat caggctgccg tggacgcgat
     3241 gcgcagcgtc cttaactcac tccaaatgga cggcatcgtc gtgatcggcg aaggcgccaa
     3301 ggacgaggcg ccgatgttgt tcgtcggcga ggaagtcggc gccgggggcg cgccacacgt
     3361 ggacatcgcg gtcgacccga tcgatggcac gcggctgctt tcgctcggta tgccgaacgc
     3421 gatcgcggtc gtggccgtgg ccgagcgggg cacgatgttc gcctcgccgg acatcagcta
     3481 catgaacaag atcgcggtag gcgcggaagc ggccggcttt gtcgatatca aggccccggt
     3541 tcaggagaac ctgcgccgga tagccgccgc gaaaggcgtc acgatcggcg atctgaccgt
     3601 tgtcgtgctg gaccg
//
LOCUS       DASSGX010000076         2078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_14718, whole genome shotgun sequence.
ACCESSION   DASSGX010000076 DASSGX010000000
VERSION     DASSGX010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14718"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VFI12_01290"
     CDS             complement(<1..312)
                     /locus_tag="VFI12_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET7055060.1"
                     /translation="MSIEDDVALLERVPTLRLLGAAPLRMLAIGSEQRDFVRGDVLFN
                     QGDDADAGFVVQRGAFRVDDGAGAEMIAGPGTLIGELALVVPMKRPSSAVALEHSSVI
                     RV"
     gene            complement(376..1062)
                     /locus_tag="VFI12_01295"
     CDS             complement(376..1062)
                     /locus_tag="VFI12_01295"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7055061.1"
                     /translation="MANARKILIVDDDSDLRDTLVEQLSLHEEFEASAVDTGAKGASA
                     AKANAPDLVLMDVGLPDTDGREVVRSLRKGGFKAPIIMLTGHDTDSDTILGLESGAND
                     YVAKPFRFAVLLARIRAQLRQHEASEDAVFSVGPYSFRPGSKMLTAANARKVRLTEKE
                     TAILRFLYRAGQMPVSRETLLQEVWGYNSGVTTHTLETHIYRLRQKIEKDAANPEILV
                     TEAGGYKLVP"
     gene            1241..1825
                     /locus_tag="VFI12_01300"
     CDS             1241..1825
                     /locus_tag="VFI12_01300"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HET7055062.1"
                     /translation="MKSTSRRCMKKQISSASYPKSKPLATIRVRAAAGNPCRGWLTAG
                     PLTIPVALGRGGIRANKREGDGGTPKGQFRPRQLWWRGDRHCRPRTHLPVRTIASTDA
                     WCEDPADRHYNRPIRRGEGQGGDRLTRADHLYDFIVEIDHNTRPRIPGRGSAVFLHLA
                     RDNFGPTAGCVAMTRAAMLQLLRRLGPRTRIMIG"
     gene            1832..>2078
                     /locus_tag="VFI12_01305"
     CDS             1832..>2078
                     /locus_tag="VFI12_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055063.1"
                     /translation="MDKDRIAAASSMLVKHWREGTKIEALEGPLRPRTRAEGYAIQAA
                     LESQSKARLFGWKIAATSEAGQKHINVAGPMAGRIMSD"
BASE COUNT          389 a          651 c          695 g          343 t
ORIGIN      
        1 gacgcggatg accgaggagt gctccagcgc gaccgcgctc gagggacgct tcatcggcac
       61 gaccagagcg agctcgccga tcagagtacc gggaccggcg atcatctcgg cgccggcgcc
      121 gtcatcgacc cggaatgcgc cgcgctggac cacgaagccg gcgtcggcgt cgtcgccctg
      181 gttgaacagg acgtcgccgc gcacgaagtc gcgctgctcg gagccgatcg ccagcatgcg
      241 caacggcgcg gcccccaata gacgcaaggt cgggacacgc tcgagcagcg ctacgtcgtc
      301 ttcgatcgac attcaggtcc gaggcccacg ggacccgcgc gctccgaaat gattcgcggg
      361 gtctcgatca gcgtatcacg gcaccagctt gtagccaccg gcttccgtca ccaggatttc
      421 cgggttggcg gcatccttct cgatcttctg gcggaggcgg tagatgtggg tttccagcgt
      481 gtgggtggtg acgccggaat tgtagcccca gacctcctgc agcagggtct cgcgcgagac
      541 cggcatctgg ccggcccggt acaggaagcg caggatggcg gtttccttct cggtcagccg
      601 caccttgcgc gcattggccg cggtcagcat cttcgagccg ggccggaagg agtagggacc
      661 gaccgagaac acggcgtcct cgctggcctc atgctggcgc agctgggcgc gaatgcgcgc
      721 caacagcacc gcgaagcgga agggctttgc gacatagtcg ttggccccgg attccaaacc
      781 caagatggta tcggagtcgg tgtcatggcc ggtcagcatg atgatcggag ccttgaagcc
      841 gcccttgcgc agggagcgca cgacctcgcg gccgtcggtg tcgggcaggc ccacatccat
      901 caggacgagg tcgggggcat tggcctttgc agcgctcgcg cccttggcgc cggtgtcgac
      961 ggccgaggct tcgaactctt cgtgcagcga taattgctcc accagcgtgt cgcgcagatc
     1021 ggaatcgtca tccacgatca ggatcttgcg agcattggcc atggggtcat ccttttgggg
     1081 gtcgtaccgg acgcatgcgc atgcgcccag ccgcaatgga tagtcagtga gaggtcgccg
     1141 gatttattgt tgtacgtgtt gaagtagtgg gctgttaccg attctcaagc cagaaccact
     1201 ctaactcaga ataacaggtc attttgatga atgtcctgta atgaaatcga catcgcgccg
     1261 ttgcatgaaa aagcaaatta gttcagctag ttatccgaag agtaaaccgc tcgcgacgat
     1321 ccgggtgagg gcggcggccg ggaatccgtg ccggggctgg ctgaccgccg ggccgctcac
     1381 catcccggtc gcgttgggcc gcggcggcat ccgtgccaac aagcgcgaag gcgacggcgg
     1441 cacgccgaag ggccaattcc ggccgcggca attgtggtgg cggggggacc ggcactgccg
     1501 gccgcggacg catctgccgg tccgcaccat cgcatcaacc gatgcctggt gcgaggaccc
     1561 tgccgatcgc cactacaacc gcccgatccg gcgcggcgag gggcagggcg gtgaccggct
     1621 cacgcgcgcc gaccacctct atgacttcat cgtcgagatc gaccacaaca ccaggccacg
     1681 cattcccggc cgcggcagcg ccgtgttcct gcatctggcc cgcgacaatt tcgggccgac
     1741 ggccggttgc gtcgccatga cgcgtgcggc gatgctgcaa ttgctgcgca ggctgggacc
     1801 acgaacgaga atcatgatcg ggtgaaggac gatggacaag gatcggatcg cagccgcgtc
     1861 gagcatgctg gtcaagcatt ggcgcgaggg caccaagatc gaggcgctgg aaggtccatt
     1921 gcgtccgcgc acgcgggccg agggctacgc aatccaggcc gcgctcgaat cgcagtcgaa
     1981 agcaagattg ttcggctgga agatcgcggc aacgagcgag gccggacaaa agcacatcaa
     2041 tgtcgccggt cccatggccg gccgcatcat gagcgaca
//
LOCUS       DASSGX010000077         3137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_449603, whole genome shotgun sequence.
ACCESSION   DASSGX010000077 DASSGX010000000
VERSION     DASSGX010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449603"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VFI12_01310"
     CDS             complement(<1..411)
                     /locus_tag="VFI12_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HET7055064.1"
                     /translation="MSSSESGVGNRIWVFDTTLRDGEQSPGATMTVEEKLEMADALVA
                     LGVDIIEAGFPAASPGDFEAVRMIAKRARGAVIAGLARANQNDIERAATAVKEAEQSR
                     VHTFIATSDIHLEHKLRMTREQVLERTAAMVALAK"
     gene            complement(445..1551)
                     /gene="ilvC"
                     /locus_tag="VFI12_01315"
     CDS             complement(445..1551)
                     /gene="ilvC"
                     /locus_tag="VFI12_01315"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF004017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HET7055065.1"
                     /translation="MQAGSSGSPFEFGNRVANGRPKHREGAFVAATLYYDQDASLDPL
                     VGKTIAIIGYGSQGHAHAQNLRDSGMKVIIGLAEGSKSRAKAEADGFEVFSVADAAKK
                     ADLIMILAPDQNQGKIYEESIKDNLAPGKTLLFAHGFAIRFGFVSPPKDVNVVMVAPK
                     APGHRFRELFQEGAGVPGLVAVEQDATGNAKEIALAYAKGLGLTRAGVIETTFKEECE
                     TDLFGEQAVLCGGMTALIQAGYETLVRAGYQPEIAYFECLHEVKLIVDQIYENGISYM
                     HYSVSDTAEYGSYYAGPQIVDEHVRQSMQSLLDRIQDGTFAKQWMAENESGREHFLAM
                     RAESQNSQIEQVGKGLRAMMPWLHKGKTPVDISA"
     gene            complement(1563..2126)
                     /gene="ilvN"
                     /locus_tag="VFI12_01320"
     CDS             complement(1563..2126)
                     /gene="ilvN"
                     /locus_tag="VFI12_01320"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HET7055066.1"
                     /translation="MESTKPRTQRHTLVVLVEDHPGVLNRVVSLMRRRSFNIHTITVG
                     ASEQPGVSRMTLVVEGDAQAVEQAGKQLYKLLEVLKVTDITDTNSISHELVLVKVATK
                     PQNRQEVMLAGQIYGAKFVDATPSSLIMEVTGSPERIDSFLNMVKPYGIRELVRTGPV
                     AMSRGGGTVQIRDGRIDRATGVPAAAD"
     gene            complement(2132..>3137)
                     /locus_tag="VFI12_01325"
     CDS             complement(2132..>3137)
                     /locus_tag="VFI12_01325"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET7055067.1"
                     /translation="VERTGIPVIFTLLGQGSFPESHRLSLGLMGMHGHREVNMALKEC
                     DLLVNIGARFDDRATGKVSTFLPNARVVHVDIDPAEIGKNIPTDVPVVGDAREALKLL
                     LGEVNERDHSDWLAWIMSQKDTALREALNDRPEWPEPYTIIKEIANVTKGEAIYCTDV
                     GQHQMWAAQHLGLDHPKRWLTSGGLGTMGYGLPAAIGAKMGRPDLEVWTIIGDGGFQM
                     SSNELATCVQEGLDINIAVINNGYLGMVRQWQDLFHQKNYSEVQILGPDFVKLAEAYG
                     CTGIRVTRDDEIVPAIEKARSINGPVLIEFVVEPEANVWPIVPPGASNADMMLNRGEE
                     "
BASE COUNT          511 a         1036 c          967 g          623 t
ORIGIN      
        1 cttggccagc gccaccatcg ccgccgtgcg ttccagcacc tgttcgcgag tcatccgcag
       61 cttgtgctcg agatggatgt ccgacgtggc gataaaggtg tggacgcggg actgttcggc
      121 ctccttgacg gcggtcgcgg cccgctcgat gtcgttctgg tttgcgcgag ccagtccggc
      181 gatcaccgcg cccctggccc gcttggcgat catgcggacc gcctcgaagt cgccgggcga
      241 ggcagccggg aaaccagctt cgatgatgtc cactccgagg gcaacgaggg catcggccat
      301 ctcgagcttc tcttcgaccg tcatcgtcgc gccgggcgac tgctcgccgt cgcgcaatgt
      361 ggtgtcgaag acccagatgc ggttacctac gccactctcc gaactggaca tcactgaact
      421 ccttttcgcc ctgcctgcct gaacctacgc ggaaatgtcg acgggggtct tgcccttgtg
      481 caaccacggc atcatcgcgc ggagcccctt gccgacctgc tcgatctgac tgttctgcga
      541 ttcggcgcgc atcgccagga agtgctcgcg gccggactcg ttctcggcca tccactgctt
      601 cgcgaatgtg ccgtcctgga tccggtcgag aagcgactgc atgctctgcc gaacgtgctc
      661 atcgacgatc tgggggccag cgtagtaaga cccatactcg gccgtgtcgc tgaccgagta
      721 gtgcatgtag gagatgccgt tttcgtagat ctggtccacg atcagcttca cctcatgcaa
      781 gcactcgaag tacgcgattt ccggctggta gccggccctg accagcgtct cgtagccagc
      841 ctggatcagc gcggtcatgc cgccgcagag gaccgcctgt tcgccgaaga ggtcggtctc
      901 gcactcttcc ttgaacgtgg tctcgatgac gccggcgcgg gtcagcccga gccccttggc
      961 ataggccagc gcgatctcct tggcgttgcc ggtcgcatcc tgctcgaccg cgacgaggcc
     1021 cggcacgccg gcgccctcct ggaagagctc ccggaagcga tggccgggcg ccttcggggc
     1081 gaccatgacc acgttgacgt ccttcggcgg cgagacgaag ccgaaccgga tcgcgaagcc
     1141 gtgcgcgaac agcagcgtct tgcccggcgc gaggttgtcc ttgatcgact cctcgtagat
     1201 cttgccctgg ttctggtcgg gcgcgaggat catgatcagg tcggccttct tcgcggcatc
     1261 ggcgaccgag aagacttcga agccatctgc ctcggccttg gcccggcttt tgctgccctc
     1321 ggcgagaccg atgatgacct tcatgccgct gtcacgcagg ttttgcgcgt gggcatggcc
     1381 ctggctaccg tagccaatga tcgcgatcgt cttgccgacg agcggatcaa ggctggcgtc
     1441 ctggtcgtaa taaagggtgg ctgccacgaa agctccttct ctgtgctttg gtcgcccgtt
     1501 cgcgacccgg tttccaaatt caaacgggga gccgctgctc cccgcctgca ttctcgcaaa
     1561 tctcaatcgg ccgcggcagg tacgccggtg gcccggtcga tccggccgtc acgaatctgt
     1621 acggtgccgc ctccccggga catcgccacc gggccggttc ggaccagctc gcggattccg
     1681 tacggcttga ccatgttcag aaacgagtcg atccgctccg gcgaaccggt gacttccatg
     1741 atcagcgacg acggtgtggc atcgacaaac ttcgccccgt agatctggcc ggccagcatc
     1801 acctcctggc ggttctgcgg cttggtcgcg accttgacca ggaccagctc gtggctgatc
     1861 gagttcgtgt cggtgatgtc ggtgaccttc agcacctcca gcaacttgta gagctgcttg
     1921 ccggcctgct cgacggcctg ggcatcgccc tcgacgacga gcgtcatccg cgacacgcca
     1981 ggctgctccg atgcaccgac ggtgatggta tggatgttga aggagcgtcg ccgcatcagg
     2041 gaaaccaccc ggttcaggac gccgggatga tcctcaacca gcacgacgag cgtgtgccgc
     2101 tgtgtcctcg gtttcgtcga ttccattgcc gttactcctc gccccggttc agcatcatgt
     2161 cggcattgct cgcgcccggc ggcacgatcg gccagacgtt cgcttccggc tcgaccacaa
     2221 attcgatcag gaccggcccg ttgatcgacc gcgccttctc gatcgccggc acgatctcgt
     2281 cgtcccgcgt cacgcggata ccggtacagc catacgcttc ggccagctta acgaagtccg
     2341 gaccgaggat ctggacttcc gaatagttct tctggtggaa tagatcctgc cattggcgaa
     2401 ccataccgag gtagccgttg ttgatcacgg cgatattgat gtcgagccct tcctgaacgc
     2461 acgtcgcgag ctcgttcgac gacatctgga atccgccatc gccgatgatc gtccagacct
     2521 cgaggtccgg ccggcccatc ttggcgccga tcgcggccgg caggccgtag cccatcgtgc
     2581 cgagtccgcc ggaggtcagc cagcgcttcg gatgatcgag gccgaggtgt tgggcggccc
     2641 acatctgatg ctgcccgacg tcggtgcagt aaatcgcctc gcccttcgtg acattggcga
     2701 tctccttgat gatcgtgtac ggctccggcc attcggggcg gtcattcagc gcctctcgca
     2761 acgcagtgtc cttttgggac atgatccagg cgagccagtc gctatgatcg cgctcgttga
     2821 cctcgccgag caagagcttc agcgcctcgc gggcgtcgcc cacgaccgga acatcggtgg
     2881 gaatgttctt gccgatctcg gcgggatcaa tatcgacgtg gaccacgcgc gcattcggca
     2941 ggaaggtcga aaccttcccg gtggcgcggt cgtcgaaccg ggcgccgatg ttcaccagca
     3001 ggtcgcactc cttgagcgcc atgttgacct cgcggtggcc atgcatgccc atcaggccga
     3061 gcgagagccg gtgcgactcc gggaacgagc cttggccaag gagcgtgaag ataaccggga
     3121 tgccggtccg ctcgacg
//
LOCUS       DASSGX010000078         5433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_449941, whole genome shotgun sequence.
ACCESSION   DASSGX010000078 DASSGX010000000
VERSION     DASSGX010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5433
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449941"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            455..736
                     /locus_tag="VFI12_01330"
     CDS             455..736
                     /locus_tag="VFI12_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055068.1"
                     /translation="MRARTAVSDSPRFRVELHEGRTITVLGFLPNGFAHHSTLAPYVT
                     RLVEQGVTGWLVLVDTESEGPVARRLVRPRPRPHQLLRARPPRSPVPRD"
     gene            1519..2316
                     /locus_tag="VFI12_01335"
     CDS             1519..2316
                     /locus_tag="VFI12_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055069.1"
                     /translation="MRPTLVSWPRLRLITFACWLLLTLVAAAATGYAVWHVTGTTPHR
                     ERAGGAPSTDTGLVEATATRTPTLRVIRLTPDPSFGTIAGPTTTAPSARSEHGSNPRS
                     VTPTQSGPAVPSPSATPAAPELVTNGSFEAGASAWYLEDDAGPVAAPTTVDGTMVLQI
                     PSGGGYADQHVAAAAGETYQLIGSGRITVEGDAGTLGVVYRDAAGTRLTALEPPPMVF
                     TRTRFRAKRLTFTVPAGVVDVLVYAYKEPGSGQFEADAISVRHVEEP"
     gene            complement(2629..3165)
                     /locus_tag="VFI12_01340"
     CDS             complement(2629..3165)
                     /locus_tag="VFI12_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055070.1"
                     /translation="MLPSAIAWPERPARRVAPVFRLLPASLNKIFYHAHRRTEMPCAR
                     GIAVYAKLIVWRLGAEYRDPSGYERFLRGFAPDALPQLRPFGMLDGFIIRLTHSTVLT
                     VNLYETADGAYSAWHTVVSQPEYGKAGDLEVLFHLICRGDDLPLAGEIFGTDSDGDSG
                     AEDPADTGASDQPVSVER"
     gene            complement(3137..3355)
                     /locus_tag="VFI12_01345"
     CDS             complement(3137..3355)
                     /locus_tag="VFI12_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055071.1"
                     /translation="MLTGSLVLLGISLLWLAVIIIHGLRCNARERAAEQPREPVTPIQ
                     RPVACGDVLHPPPDLYRPGHAAERHRVA"
     gene            4480..5205
                     /locus_tag="VFI12_01350"
     CDS             4480..5205
                     /locus_tag="VFI12_01350"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET7055072.1"
                     /translation="MSAPRGRPRPGVNRLVALLPPDSRERLLAHAQPVQLGLGHVVWP
                     QDHSITALFLPTTAVFSLVVRMDDGRGVEAAPVGFEGAVGTALLLGESRSPCEVVIQG
                     AGHALRIPAPTALRLAAEDPAIRDVLLRSAQVQMVQASRSAACNRLHPVEERLARWLL
                     HMHDWIWAEELHLTHEFLAIMLGVRRASVTMAAGILQQAGLIGYQRGAITVLDRTKLE
                     DVACEDYFAVRDAFERLLPLPPG"
BASE COUNT          902 a         1857 c         1766 g          908 t
ORIGIN      
        1 ctggccggca gcgcggcata gccccagtcg taccagcgag cgaccgcggt aaccgatgcc
       61 cctgttcgat gggaacgagg atcgtccggt gccggtttgg ataccggtcc tgctgataaa
      121 tcaatgtcaa agacgagatc catgatgccc ccgggaccgt agccgaccgc gtgtggctcc
      181 gagcataggt cggacaagcc gaaccgggag ggggcggcta ggactaccga cggggtcgga
      241 ccggtacctt cgtcatgccg ggcgtggaag tagcacggcg ttgcgggtac aggccgatcg
      301 gggagtagcg ttccaggtcc cgcgggtctc ccgcggccaa cggattcgcc gcccgcggtc
      361 gtgtggacgc ctgcgttcag tacaaaggtc ccgccatttc tccccgccga tcacccatac
      421 cggctagccc acccggcgca tacgctcggt cccgatgaga gcgcgaaccg ccgtatcgga
      481 tagcccccgc ttccgcgttg agctccatga gggtcgaacc atcacggtgc tcgggttctt
      541 gccgaacggc tttgcccacc actcgaccct cgcgccctac gtcacccgtc tggtcgagca
      601 gggcgtgacc ggctggctgg tgctggtcga cacggagagc gagggcccgg ttgcccgccg
      661 gctggtgcga ccccggcccc ggccgcacca gctgctccgg gctcggccgc cgcgcagccc
      721 ggtcccgcgc gactaagggc gcggcaacgg ctcagcgcgg agcggctgct tgggcgggcg
      781 atgcggcgag acatggcggg tgccccatcc ccgcccagca catgcccggc tcgcatagcg
      841 gcgatggcgg ccgcgccggt ccggggacag gacgtgccga ccacctccgc aggcgcaact
      901 ccgccatatc gcctccctac cccgattccc catgaggatg ctcctttgca ccatggaggg
      961 attgccccag tgcctaaccg cgctggggtc aagattagtc gtagcacgac acgggtgggc
     1021 atcgcggatg cgcggcacgc tgctatgtca tgttcgatac cggacgcggg ccaggtggcc
     1081 cccttgcgct cgggtgttgg ctgggcacgg cggcaggggc gcgagtcacg accacttccg
     1141 gcaccgttac cgaccgcgcc agcccggcac attcggcaga cggtcaccgg actgttcgtg
     1201 gcagctggtc aaagtcgccg atccacccag ttcccagggc cggcattgac tcccgccgat
     1261 aggatgggaa ggcggggagc agtcgagacg agtcgcgtca ctgatcccag gcaatcatca
     1321 gccccgggcg ggccacaaca ccgcccgggg tttcttgtac ccgattccta ttccgatatg
     1381 cccagatttc aatacgatag cggcggtgca ggccgcgggt gcggccggca gcggttacct
     1441 cgctcacggg ctgcctgggc cactcggcag cccgtgagtg ttttgttctt ccgaactggt
     1501 gtccgagcag ggaacactgt gcgacccacg cttgtcagct ggccccgtct ccgcctcatc
     1561 accttcgcct gttggctcct gctgaccctc gtcgccgccg ctgccaccgg gtatgccgtt
     1621 tggcacgtga cgggtacgac tccccatcgc gagcgcgctg gcggcgcacc gtcaaccgac
     1681 accggactcg tggaggcgac cgccacacgt accccgaccc tgcgggtcat tcggctcacg
     1741 cccgacccgt cctttgggac gatcgcgggg ccaaccacga cggcaccttc agccaggagc
     1801 gagcatggat cgaatccgcg ctcagtgaca cccacgcaga gcggtcccgc tgtgccgagc
     1861 ccgtctgcca cccccgccgc gcccgagctg gttactaatg gcagcttcga ggccggcgcg
     1921 tctgcctggt acctcgagga cgacgcgggc ccggtcgccg cacccacgac ggtcgatggg
     1981 acgatggtgc tccagatccc gagcggcgga ggctacgccg atcagcacgt cgctgccgcc
     2041 gccggcgaaa cctatcagtt gatcgggagc ggccggatta cggtcgaggg cgacgcgggg
     2101 acgctgggcg tggtctaccg ggatgcggcg ggtactcggc tgacagcgct cgaaccgcca
     2161 ccgatggtgt tcacccggac gcggttccgg gcgaaacggc tgacctttac ggttccggca
     2221 ggcgtcgtcg acgtgctggt ctatgcgtac aaggagcctg gttccggcca gttcgaggca
     2281 gacgcgatct ccgttcgcca cgtggaggag ccgtagcgga cagggtggat ggggtagcgc
     2341 ctccgcccgc tgttctcccg ctctaatcct ctgccatttg cccggatggt gtcgtgtttg
     2401 cagccgacaa cacagtctgc ttgcaaccgc ggtatccggc cagcaaagcg ccacggtccg
     2461 gtccaggatc gcgccacccg atggcgcccg gcggcatggc cgggtgcagt ggcgaccaga
     2521 acgtccgcgt agtggaagcg ggcgcgacga gccggaagat cgcgatggcg gcagcctaga
     2581 aagtacccag ggagctcaat ccggctcgag cgaaccgggg catacggttc accgctcgac
     2641 agagaccggc tggtcgctgg cgccagtgtc cgccggatcc tcggcgcccg aatcaccatc
     2701 ggagtccgtg ccgaatatct ccccggctaa gggcagatca tccccccggc aaatgaggtg
     2761 gaagagcacc tcaagatcgc cggccttccc gtactcgggc tgactgacga cggtgtgcca
     2821 ggccgaatag gcgccgtcgg ccgtttcgta caagttgacc gtcagcaccg tgctgtgcgt
     2881 cagccgaatg atgaagccat cgagcatgcc gaacgggcgc aattggggaa gcgcatccgg
     2941 cgcgaagccg cgaaggaacc gctcatatcc cgacgggtca cggtattcag cacccagtcg
     3001 ccaaacaatc aatttcgcgt acacggcgat gccccttgcg catggcattt cggttcgacg
     3061 gtgcgcatga tagaaaattt tgttcagcga tgcgggaagg agccgaaaca ccggcgcgac
     3121 cctgcgggcc ggacgctcag gccacgcgat ggcgctcggc agcatgaccg gggcgataca
     3181 gatcgggcgg tgggtggagc acatcgccgc aggcgaccgg gcgctggatg ggcgtcaccg
     3241 gttcgcgggg ttgctcggcc gcccgctccc gcgcattgca gcgcagtccg tggatgataa
     3301 tgacagccag ccagagcagc gaaatgccaa ggagcacgag tgagccggta agcatggcct
     3361 acaaccaaac gacttggatg ggcgcgattg ccacatgccc gctcggcggc gcccctcaac
     3421 tccgtcgttc gcggtaaggg tagtttgctt agttccgcaa tcacagctta caaaagtacc
     3481 agtcgtggcg caccgcaact tgcgtcgcca aagctggcgg atcgttggcc gctcacctgt
     3541 tctcttgccg gaaggcgtag gatgggattg tctccactcg ttcccaactc ggcctcctca
     3601 ctgctccggc acctcgttgg cgccacgagc gcagccgtcg ggttggagcg ggaagggaac
     3661 aacggtcacc cggcccgttg cggcggattc ccccgggtga ccgctccccc tcgcccgtgc
     3721 acatcccgtt cgaatcgttc actgtagatt ccgggacggt cactcggacg ggcgggaggg
     3781 ggatccttcc cggccgagtg actctcctca tcctggccac caggacgacc gcttcgggaa
     3841 cccgaccgaa ctgacctgcc tccggccgga ggaagcgcag atgcgctccc ctcggacggt
     3901 cgtgcccggc gcacatgccc ctcggtacgg caccgtaccg actagaggat tggactcgga
     3961 tagagttgcc atgactcgtg ggaggatgcc cgtcgagctg ccgcggacac aacacgttgg
     4021 cagccctcat cgtgcacccc ctttgccatt gaggacagcc ccccaatccg ggttccaggc
     4081 aacgtgacag ccagcgttgc cctcctccct cgtgtcaccc ctcctccttg actctgttcg
     4141 tacactaggc aaagcaccag gtgatgcatc ccacgaggtg cttcgcggtt ggctggcgtg
     4201 gagttcgcca tccctcgcgt ggtgatccgc ctggtgcgtt gcccggggtg ctactccgct
     4261 cgcaccagat ctcgccactt gactacggga tcgccgtgcg tttcgcggat cgacgaaccc
     4321 aagcggcgta ccgtgtcgag gcaatccctt tgcatggtgc acagcttctg gtattgaagg
     4381 tgggtaggtg gtaggctcgc ctctgatcgt agccgcacgc ccatcccaca cggccgctcc
     4441 cgggtcctcg gacgcgtgga gcagctcagg agctccgcca tgtccgcacc acgcggccgc
     4501 ccccgcccgg gcgtcaaccg cctcgtcgcc cttctgccac ccgattcccg ggaacgcctc
     4561 ctcgcccacg cccagccggt ccagcttggc cttgggcacg tcgtgtggcc gcaagatcat
     4621 tcgatcaccg cgctctttct gccaacgacg gcagttttct cactggtcgt gcgcatggac
     4681 gacggccgcg gcgtggaggc ggccccggtc ggcttcgaag gcgcggtcgg cacggccctg
     4741 ctcctcggcg aatcacggag cccatgcgaa gttgtcatcc aaggcgcagg ccacgccctg
     4801 cggatcccgg cacccaccgc gctccgtctc gccgccgagg acccagcaat ccgggacgtg
     4861 ctcttgcgct cggcccaggt tcagatggtc caggcgtccc gcagtgccgc ctgcaaccgg
     4921 ctgcacccgg tggaggagcg gctggcccgc tggctgctcc atatgcacga ctggatctgg
     4981 gcggaggagc tgcacctgac ccacgaattt ctcgcgatca tgctcggcgt gcgacgggcg
     5041 agcgtgacca tggccgccgg catcctgcag caggcggggc tgatcgggta tcagcggggc
     5101 gcgatcacgg tcctcgaccg gaccaagctg gaagacgtcg cctgcgagga ctatttcgcg
     5161 gtacgggacg cattcgagcg gctactacca cttcccccgg ggtgaaaccg gctcaaaatc
     5221 tccgccaccc ggccaaagga cgagcgtgcg tccgagcggc tattcccctt ccggtacggt
     5281 ggcaggacac ggcgttcaga ggcggtaagc gcagttcagg ctacgctgcg tgtcagcaac
     5341 ggtagtccgg cgttcaggtc tgggtatcac aaactgttga atcagaccac ggcagtcact
     5401 aggctgagcc gctcatgctg gaggggattc cgc
//
LOCUS       DASSGX010000079         6854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1102912, whole genome shotgun sequence.
ACCESSION   DASSGX010000079 DASSGX010000000
VERSION     DASSGX010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6854
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1102912"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="VFI12_01355"
     CDS             <1..669
                     /locus_tag="VFI12_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055073.1"
                     /translation="AYYWSSVDPDTHPDYLGKLQAMAEAVHDSDGLWIAPAAPGFDAR
                     LIGGERTIDRKDGQTLTTEMKTALQSKPDAIGLISWNEFSENSQIEPSCEYGSQSLAV
                     IAEIEDGTLATDIPTACPEGTPTTTTATAKSNGTVVAQAVTVTAVPTRAPTMSPTPWP
                     TSKVSTGFVDPVRPDANSDYDSSSPAATDRTIGRMARIGMLISIVMFLVALAIVRARH
                     SRAL"
     gene            721..1842
                     /locus_tag="VFI12_01360"
     CDS             721..1842
                     /locus_tag="VFI12_01360"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET7055074.1"
                     /translation="MSVAFFVILVQFFVRRDDHTVSAPEQLTGAAMFRVNTERTGAGV
                     SANGTPKPKLAWKFQAGSEVNGSPVVMDGILFVTGNDNVVYAFDADSGKPLWNASIGS
                     GINENGIISSPAVANGTVYVGGWDGIVHAFDAATGQVRWQFHADGRMVSSPVVTNGVL
                     IVGTNGGTVHAINAATGDRVWDAALGTRVLSSPAVTGGLVIIGSGKSLLALDIASGRV
                     HWLYRTDGEVSSTPAIANGVAYFGSADNSLYAVDVSRGRVRWSFKTGGDVQSSPAVAG
                     GIVFVGSADGNVYAVNAETGAEQWQFATGNIVDSSPTVAGGTVYVGSVDGNLYAIDAV
                     AGTERWRFETNGPIISSPTVIGNRLFVGSGDLFLYALTG"
     gene            1987..2727
                     /locus_tag="VFI12_01365"
     CDS             1987..2727
                     /locus_tag="VFI12_01365"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET7055075.1"
                     /translation="MTDSVRSGPLPRYYQLREIIRERIMSGEWAPGSLIPSERELCER
                     YGISRMTARQSITELVNEGLLYREQGKGTYVGQPKIAQQLLRLTGFTEDIHSRAQRPG
                     AKVLSAEMWPADEATAARLRVKVGQPVYRLRRLRLADSRPLALETSCVTFIGCERLLD
                     RDLERDSLYRLLTDTYDQPPLEAEQELEADLAGEEESRLLEVPVGSPVLRTRRLTTTR
                     RNQPVEYATSVYRGDKYRFHTRMVRDLS"
     gene            2823..4166
                     /locus_tag="VFI12_01370"
     CDS             2823..4166
                     /locus_tag="VFI12_01370"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7055076.1"
                     /translation="MTNSDTTGNRLHMTRLTRRRFAQGLAATPFALGAATRGFRTAYA
                     QDKVKLVVLTHWGTAEQKDPLEKIFAEYGQTNPNVQIELQTVAFDDLLNRITTGQLGG
                     DAPDIYHFYNLWLPDFVGSELLSATPDDVSSDVSAAYGAGTVGGASYNGKVWGYPTEV
                     NDYQLIYNKKAFEEAGVSAAPVTFAEFRDTAQKLTKKDSGGKVTQAGFMFLSGWDSGV
                     VHPWTSLLWSNGGQYVADDYSKALFNSQAGIDTLQLETDIVNDGSAILAKLEDADFEA
                     GRVGMTIMANWWGATLRASNLGIDNVGVAPIPHGDGGSSVALQYEWLWGVGNTSQRSE
                     DAWTFLKWLNSPRDGAEASSPMGEFLTSALNAIPGRTSDQTAHADLLSDPFVKPFVDA
                     FATSRSEPIIPGAQEIKTGLQKQIEAAWAGQESPTDALNKAAEDADRILKEKAAS"
     gene            4191..5165
                     /locus_tag="VFI12_01375"
     CDS             4191..5165
                     /locus_tag="VFI12_01375"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET7055077.1"
                     /translation="MPPQNLINRFGVEMGAVTTAEQVLVRPGRVRTRRRVSRHSMAGY
                     AFVAPALILLIVFALGPFLFTIWVSVHSWNMLTPLTTMPFQGTGNYSYLLRDDALFRE
                     TFGNTVYFAIGNVVVSVVLALSVALILNGPIRLRAFWRAAFFMPYVTAPVAIAIVWRN
                     MLDSNWGLVNGLLARAGLPEQPFLDSLSQAMPSLIGVSVWHEVGYYMIIFLAGLQAIP
                     YDLYEAAKLDGASTWRLFWSITLPLLRPTLLFVVVVITLGSLQVFDLPFILTQGGPVN
                     STNTLVMYMYETAFKFLRMGRATAMAVMLFGVIFTMTMIQLRLLRERE"
     gene            5162..6007
                     /locus_tag="VFI12_01380"
     CDS             5162..6007
                     /locus_tag="VFI12_01380"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET7055078.1"
                     /translation="MNSSYAIRPTITTGLKYLFLIIGATVMLLPFAWMILSSMMTANE
                     ILTRPLTWFPAHPGFDAYRGLRNAIPLERMYLNSMIVTTLTTLGILVTSSLAGYGFAK
                     FQFPGRDILFLIVLATMMIPFFVVLIPIFYMISKFGWINSYPGLIVPNIATAFGIFLM
                     RQYMLSLPDEVLDAARVDGASEFEIYWRIVLPLSTPVIGALGILAFVYQWNNFLWPLV
                     VARSDSMWTVPVGLNSLRVYASSAQVINMQMAGATLGIVPLVVVFLALQRYFVRGIAL
                     TGMKG"
     gene            6075..>6854
                     /locus_tag="VFI12_01385"
     CDS             6075..>6854
                     /locus_tag="VFI12_01385"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7055079.1"
                     /translation="MTKDERSRTNSEPLIRGFRSGDEAGLLATWNTSLWADPISAMTW
                     RAKVLLDPNFDPEGCLIAEVDGDVRGFLLSLVRRVPFFGQGYEPEQAWITAFGVAPGW
                     EERGLGSALLDAALERLQGLGRTSVALSPYVPNYFTPGADVVAYARGIEFLTKRGFEV
                     IERPISMRAELTGFRIPDEIQERAAALRDEGIEIRPATPADIVPVLDFIPRHFTWDWH
                     REASGIFNDLFAGDPRNVGMMVAMQDGEALGYAEHRGERFGP"
BASE COUNT         1273 a         2172 c         2123 g         1286 t
ORIGIN      
        1 gcctattact ggtcgtccgt cgacccggac acgcatcccg actacctggg caagctgcag
       61 gcgatggccg aggcggttca tgacagcgac gggctctgga tcgcgcccgc cgcgccgggt
      121 ttcgacgcgc gcctcatcgg gggggagcgc acgatcgacc gcaaggatgg gcagacgctc
      181 acgacggaga tgaagacggc gctccagtcg aagcccgatg cgatcggcct gatcagttgg
      241 aatgagttca gcgagaactc gcagatcgag ccgagttgcg agtacggctc acagtccctg
      301 gcggtgatcg cagagatcga ggatggaacg ctcgcgaccg atatcccaac cgcttgcccc
      361 gaaggcacgc caacgacaac gactgcaaca gcgaaatcca acggcaccgt cgtggcccag
      421 gccgtgaccg tcacggctgt cccaacccgc gcgccaacga tgagcccgac cccctggccc
      481 acctccaagg tttcgaccgg gttcgtggat cctgttcggc cggatgccaa ttcggactac
      541 gattccagct ccccggccgc caccgaccgc acgattgggc gaatggcccg gatcggcatg
      601 ctgatctcga ttgtgatgtt ccttgtggca ctcgccattg tccgcgcgcg gcattcccgg
      661 gctttgtagg cagccatgca ccgttcaagg tctcgcgttt tcctgacgac gggcatcgcg
      721 gtgagcgtgg cattcttcgt catcctggtt cagtttttcg tgcgacggga cgaccacacc
      781 gtctccgcac cggaacaatt gaccggcgcg gcaatgttcc gggtgaacac agaacgaacc
      841 ggcgccggcg tatcagccaa tggcacacca aagcccaaat tggcatggaa attccaggcc
      901 ggcagtgagg tgaacggatc acccgtcgtc atggacggga tactcttcgt caccggcaac
      961 gacaacgtcg tgtatgcctt cgatgccgat tcgggaaaac cgctctggaa cgcctcgatc
     1021 gggtcaggaa tcaacgaaaa cggaatcatc tcttcgcctg cggttgcgaa tggcacggtc
     1081 tatgtcggcg gctgggatgg gatcgtgcac gccttcgacg cggcgacggg gcaagtccgc
     1141 tggcagtttc acgccgacgg acggatggtc tcctctccgg tcgtgacgaa tggcgtcttg
     1201 atcgtcggaa ccaacggcgg cacggtgcac gctatcaatg ctgcgactgg cgatcgcgtc
     1261 tgggatgcgg cgctcgggac gcgggtgctt tcctctccag ccgtcaccgg tgggttggtg
     1321 atcatcggaa gcggcaagag cctcctggcg ctggatattg ccagtggccg agtgcattgg
     1381 ctctaccgga cagacggcga ggtcagctcg actccggcaa tagcgaacgg tgtcgcgtac
     1441 ttcgggagcg ccgacaactc gctctatgcg gtcgacgtgt cacggggacg agtgcggtgg
     1501 tccttcaaga ccggcgggga cgttcagtcg agtcctgcgg tcgcgggcgg gatcgtgttc
     1561 gtagggagtg ccgacggcaa cgtctacgca gtcaatgctg aaaccggcgc cgagcaatgg
     1621 caattcgcga ccgggaacat cgtcgactcg tccccgaccg tggccggcgg tacggtctac
     1681 gtcggaagcg tggacgggaa tctctacgcg atcgacgcag tggcgggcac cgagcgctgg
     1741 cgcttcgaaa cgaacggacc aatcatctca tcgcccaccg tcatcgggaa ccggctcttt
     1801 gtcggcagcg gcgacctctt cctgtacgcc ttgaccggct gaaatctccc gagccaacgg
     1861 gattgacaac gcgtccaccg gcgctatgct gtgtgtactg gtacagacaa cctgatgacc
     1921 ggaggtccga aacacctcca cgaagttctt atcggcagtg tgttttcttt ccgcgctgga
     1981 ttggcgatga ccgacagtgt ccggagcggt ccgctccccc ggtactacca actcagggag
     2041 atcattcgcg agcgcatcat gagcggcgaa tgggcgcccg gcagtctcat cccatccgaa
     2101 cgggagctgt gcgaacgcta cggtatcagc cggatgaccg cgcgccaatc aatcaccgaa
     2161 ctcgtcaacg aagggttgct ctaccgcgag cagggcaaag gcacgtatgt cggccagccc
     2221 aagatcgccc agcagctcct tcgactaacc ggcttcactg aggacatcca ctcccgggca
     2281 caacgaccgg gagcaaaagt cttgtccgca gagatgtggc cggcggacga agcgaccgcg
     2341 gcgcggctgc gggtgaaggt cggtcaaccc gtgtaccgcc tgcgtcgcct ccgcctcgcc
     2401 gactccaggc cgctggctct cgagacgtcc tgcgttacgt tcatcggatg tgagcgactg
     2461 ctcgatcgtg acctcgagcg cgattccctg taccggttgc tcactgacac gtacgaccag
     2521 ccacccctcg aggcggagca ggagctcgag gcggacctgg ccggcgagga ggaatcgcgc
     2581 ttgctggaag tgccagtcgg gagtcctgtg ctacgcaccc gacgacttac gacaacccgg
     2641 cgcaaccaac ccgtggaata cgcaacctcg gtgtaccggg gcgacaagta ccggttccat
     2701 acgcgcatgg tgcgcgatct cagctagccg acgggcggaa aggaggacgg tcgcaaacaa
     2761 cctgatcacg gaaagtcaga tttgctgtcc tcggttcaat gatgtctcac caggagctca
     2821 gtatgacgaa cagcgacacg accggtaacc gcttgcacat gacccgtttg acccgccgcc
     2881 gcttcgccca ggggttggcg gccactccgt ttgcgctcgg cgccgcgacc cgcggcttcc
     2941 gtaccgcata cgctcaggac aaggtcaaac tcgtcgtcct cacccactgg ggcaccgccg
     3001 agcagaaaga tccgctcgag aagatcttcg ccgagtatgg ccagaccaac ccgaacgtgc
     3061 agattgagct gcagacggtt gctttcgacg atctgctgaa ccggatcacg accggacaac
     3121 ttggtggcga tgcgccagac atctaccact tttacaacct gtggctgccc gacttcgtcg
     3181 gcagcgagct cctgtccgca acgccggacg acgtatcgtc cgacgtgagc gctgcctacg
     3241 gagcgggcac cgtcggcgga gccagctaca acggcaaggt ctggggctac ccgaccgagg
     3301 tcaacgacta ccagctcatc tacaacaaga aggcgttcga ggaggcggga gtctccgccg
     3361 cgcccgttac cttcgccgag ttccgggata cggcccagaa gctgaccaaa aaggattcgg
     3421 gcggcaaggt aacgcaagcc gggttcatgt tcctgtccgg ttgggattcg ggcgtagtcc
     3481 atccatggac gtcgctgctt tggtcgaatg gcggtcagta tgtcgccgac gactacagca
     3541 aggccctctt taactcgcag gccggcatcg atacgctcca gctcgagacc gacatcgtca
     3601 acgacggttc ggcaatcctg gccaagctcg aggacgccga cttcgaggct gggcgggtcg
     3661 gcatgacgat catggccaac tggtggggcg cgacgctccg cgccagtaac ctcgggatcg
     3721 acaacgtcgg agtcgccccg atcccgcacg gcgatggggg atcgtccgtc gcgctccagt
     3781 acgagtggct ctggggcgtc ggcaacacga gtcaacgctc cgaggatgcc tggaccttcc
     3841 tgaagtggct gaacagcccg cgagatggcg ctgaggcgtc gtcgccgatg ggcgagtttc
     3901 tgacgagcgc attgaatgcg atccccggcc gcacgtcgga ccaaaccgcg catgctgacc
     3961 tgctcagcga tccgttcgtg aagccgttcg tcgacgcgtt cgcgacctcc cggagcgagc
     4021 cgatcatccc cggcgcgcag gagatcaaga cagggctgca gaagcagatc gaagcggcct
     4081 gggccggtca ggaatcgccg actgacgccc tcaacaaggc tgcggaagat gccgaccgga
     4141 tcctgaagga gaaggcggcg tcgtaacgcg ctccggggcg gcatggacac gtgccgcccc
     4201 agaatctgat caatcggttt ggggtcgaga tgggtgcggt gaccacggcg gaacaggtgc
     4261 ttgtgcgacc gggcagggtg aggacgaggc gacgggttag ccgccactcc atggccggat
     4321 atgccttcgt tgcgccagcc ttgatcctgc tgatcgtgtt cgcgctcggc ccctttctct
     4381 tcacgatctg ggtgagcgtg cactcctgga acatgctgac cccgctcacc acgatgccgt
     4441 tccaggggac cgggaactac tcctaccttc tcagagacga cgccctcttc cgcgagacgt
     4501 tcggcaacac ggtttacttc gcaattggta acgtcgtcgt atctgtcgtg ctcgccttga
     4561 gcgtcgcctt gatcctgaac ggaccgatca ggctgcgggc gttctggcga gcagctttct
     4621 tcatgcccta cgtcaccgcg ccggtggcca ttgccatcgt ctggcgaaac atgctcgatt
     4681 cgaactgggg actggtaaac gggctcctgg cccgggccgg cctgcccgag caaccgttcc
     4741 tggacagcct gagccaggcc atgccgagcc tgatcggcgt gtccgtctgg cacgaggtcg
     4801 gctactacat gatcatcttc ctcgccgggc tccaggcaat tccatacgat ctctatgaag
     4861 cggccaagct cgatggcgcg agtacctggc ggctcttctg gtcgatcacc ttgccgctgc
     4921 tgcggccgac gctcctcttc gtggtcgtcg ttatcacgct cgggtcgctg caagtattcg
     4981 acctgccctt catcctcacc cagggcggtc cggtgaactc gaccaatacg ctggtcatgt
     5041 acatgtacga gacagcgttc aagttcctgc gcatgggtcg tgctacggcg atggccgtca
     5101 tgctcttcgg tgtcatcttc acgatgacga tgatccagct ccgcctgctg agggaacggg
     5161 aatgaacagc agctacgcca ttcgcccaac catcacgacc ggattgaaat acctcttcct
     5221 catcatcggc gcgaccgtga tgttgcttcc attcgcctgg atgattctct cctcgatgat
     5281 gacagcgaac gaaatcctga ctcgaccgct gacctggttc cctgcacacc ccggcttcga
     5341 cgcctatcgc ggtctgcgta atgcgatccc gctcgagcgg atgtatctga acagcatgat
     5401 tgtcacgacg ctgacgacgc tcggcattct cgtgaccagc tcgttggcgg gctacggttt
     5461 cgccaaattc cagtttccgg gccgggacat tctcttcctg atcgttctgg ccacgatgat
     5521 gattccgttc tttgtcgtcc tgattccgat cttctacatg attagcaagt tcgggtggat
     5581 caatagctat cccggcctga tcgtgccgaa tatcgcgacc gcgttcggca tctttttgat
     5641 gcgacagtac atgctcagcc tgcccgacga ggtgctcgac gccgcccgcg tcgacggcgc
     5701 atcggagttc gagatttact ggcggatcgt cctgccgctg agcacgccgg taatcggggc
     5761 gctcgggatc ctcgccttcg tctaccagtg gaacaatttc ctctggccgc tggtcgtcgc
     5821 tcgctcggac tcaatgtgga cggtgccggt cggcctcaac agcctgcgcg tttatgccag
     5881 cagcgcccag gtgatcaaca tgcagatggc cggcgcgacg ctcggaatcg tgccgctcgt
     5941 cgtcgtcttc ctggcgctgc aacgctactt tgtgcgcggc atcgccctca ccggcatgaa
     6001 gggctaatcg agtcgtgagt cgtgagacgt gagtcgtgag caatcggacc acggaaacgg
     6061 aaaacaagga acgaatgacc aaagacgaga gatcaaggac caattcagag ccgctcattc
     6121 gcgggttccg atcgggagat gaggcagggt tgcttgcgac ctggaacacc tcactctggg
     6181 cggatcccat cagcgccatg acctggcggg ccaaggttct actcgacccg aatttcgatc
     6241 cggagggctg cctgattgcc gaggttgatg gcgacgttcg cgggttcctg ctttcgcttg
     6301 tacgccgggt ccccttcttc gggcagggat acgagccaga gcaggcctgg attacggcgt
     6361 tcggcgtggc gcctggctgg gaggagcgcg gcctcggcag tgcgctcctc gacgccgcgc
     6421 tcgaacgcct ccagggcctc gggcgtacca gcgtcgccct ctctccctat gtgccgaact
     6481 acttcacgcc cggcgccgac gtggttgcct acgcgcgggg catcgagttc ctgacgaagc
     6541 gcggattcga ggtcatcgag cgtccgatca gcatgcgggc cgaattgacc ggcttccgca
     6601 tccccgacga gatccaggag cgagcggctg ccctgcgaga tgaaggcatc gagatccggc
     6661 ccgccacgcc ggccgatatc gtgccggtgc tcgatttcat cccgcgccac ttcacgtggg
     6721 actggcatcg ggaggcgagc ggcatcttca atgacctctt cgccggcgac ccgcgcaacg
     6781 tcggaatgat ggtcgcgatg caggacggcg aggcgctcgg ctacgcggag caccgcggcg
     6841 agcgtttcgg cccc
//
LOCUS       DASSGX010000080         2423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1539281, whole genome shotgun sequence.
ACCESSION   DASSGX010000080 DASSGX010000000
VERSION     DASSGX010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1539281"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VFI12_01390"
     CDS             complement(<1..253)
                     /locus_tag="VFI12_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055080.1"
                     /translation="MRAAELRCRNDWGQDQWEAKVEGDTVLGVVMNDAPLARPSRSDR
                     DTRPRPDIDIPDIQVPAADVTASPMVPVSRMDDGPEPSET"
     gene            765..2135
                     /gene="miaB"
                     /locus_tag="VFI12_01395"
     CDS             765..2135
                     /gene="miaB"
                     /locus_tag="VFI12_01395"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HET7055081.1"
                     /translation="MGKAIIPLHPANQTAQVMGRVENPNGKRFHIWTIGCQMNEADSA
                     KVAAMLQEVGYAPTETELDADVIVLNSCVVRQAAEDKVAGKLGSLARIKRDRPDVQIV
                     LTGCMVTKQQEQLKTRFPHVDLFFDPSDFDKLQILIPELSDLHDDLGQIPHYYLPEAG
                     DSRVSAYVPIIYGCNFLCSYCIVPYRRGKERSRPLADIVLEVEGLVRQGVKDVTLLGQ
                     TVNAYGHDLPEETTLAALLRAVDAIPGLDRLRFLTSHPKYMTDELIDTMAGLPSICEH
                     LNLPVQAGDNEVLKRMRRTYTADLWRDRIAYARSKMPGLTVATDIIVGFPGERADQFQ
                     NTLDLLEEVGCDKVHVAMYSPRPGTLSARWEDDIPHDEKHRRHQAVEKLQERILTERN
                     SHRVGEVYEVLIDTQQKGRWTGRTRGNLLVHVDSHLNLLGRNVPVRITESSPWYLIGT
                     VDGAAV"
     gene            2183..>2423
                     /locus_tag="VFI12_01400"
     CDS             2183..>2423
                     /locus_tag="VFI12_01400"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7055082.1"
                     /translation="MSDTKQATRRQLIEDARLAAIEGRWGNALTINQQLIDKTPRDAA
                     AFNRVGKAQLELGNVQEAIDAYTSSLKIDPANMIAR"
BASE COUNT          496 a          765 c          723 g          439 t
ORIGIN      
        1 ctgtttcgga tggttccggc ccgtcatcca tgcgagaaac cggcaccatg ggacttgcag
       61 tcacatcggc ggcggggacc tgaatgtcgg gaatatcgat atcgggtcgc ggccgtgtgt
      121 cgcggtcgga ccgtgagggt cgcgcgaggg gagcatcatt catgacgaca ccgagaaccg
      181 tgtcaccctc gaccttcgct tcccactggt cctggcccca gtcgttgcga cagcgcaact
      241 cggcagcgcg caccaaaatc cggactgatg acgactcctg ccgttcggga tgcgtacacc
      301 agccttgttt gccgtctcgt gccgcgtcgt agtaacggca ggacccacac tcacgcttgg
      361 ccaagacgga tcccccgagc cccttcaccg cgatctgaag cgcagcgcca gacggcggaa
      421 aacaggcgtc gactcatggt caacggcccg aaatgctgtc caaccacggg taccgtatct
      481 agcgctgacg cccattgtag cgagccctca tgcgtctgtc gagacaattc ggcgttctat
      541 tttcataccg ataaaaagga gtttctacta cattctcgta tccattgggc acgcggcaga
      601 tgagtttcct gtcgaagcac ttgaggcggc cgttccgtga ctggttgaca catcacggcg
      661 gtcggcaggg gcctgccact cgcacggtgt acacttgccg gtcgtgacga cggcggacgc
      721 cgatctcgtc ctgtttcctg tgcattaccg aaccgagagc atttgtgggc aaagccatca
      781 tcccgctcca cccggcgaac cagaccgctc aagtgatggg tcgagtcgaa aatccgaatg
      841 gcaagcggtt ccatatctgg acgatcggtt gccagatgaa cgaggccgac agtgccaagg
      901 tcgcggccat gctccaggag gtcggctatg cgccgaccga aaccgagctg gacgccgacg
      961 tcatcgtgct caattcgtgc gtcgtgcgac aagcagccga ggacaaggtc gccggcaagc
     1021 tcggctcgct ggcccgaatc aagcgtgatc gacccgatgt ccagatcgtg ctgaccggct
     1081 gcatggtgac caagcagcag gagcagctca agactcgctt cccgcacgtc gatctcttct
     1141 ttgatccgtc cgacttcgac aagctccaga tcctcatccc ggaactgtcg gacttgcacg
     1201 acgatctcgg ccagatcccg cactattacc tgccggaagc gggcgatagc cgggtatcgg
     1261 cctacgtgcc gatcatctac ggctgtaact tcctctgctc gtactgcatc gtgccctatc
     1321 ggcgcggcaa ggagcgcagc cggccactcg ccgatattgt gctcgaggtc gaagggctcg
     1381 tgcggcaggg agtcaaagat gtgacgttgc ttggccagac agtcaacgca tacggtcacg
     1441 accttcccga agagaccacg ctggccgcgc tgcttcgcgc tgtcgacgcg attccgggac
     1501 ttgaccggct ccggttcctt acctcacatc ccaagtacat gaccgacgag ctgatcgaca
     1561 cgatggccgg gttaccgtcg atctgcgagc acctcaacct gccggtgcag gccggtgaca
     1621 atgaggtcct gaagcggatg cgccgcacgt atacggccga cctatggcgg gatcggatcg
     1681 catatgcccg ttcgaagatg ccgggcctga cggtcgcgac cgacatcatc gtgggcttcc
     1741 cgggcgagcg ggccgaccag ttccagaaca ccctcgactt gctcgaggag gtcggctgcg
     1801 acaaggtcca cgtcgcgatg tactcgccgc gtccgggcac actctccgcg cgctgggagg
     1861 atgacatccc gcacgacgag aagcaccgcc ggcatcaggc cgttgaaaaa ctgcaggagc
     1921 gcatcctgac cgaacgcaac agccaccggg tcggcgaggt gtacgaggtc ctgatcgaca
     1981 cacaacaaaa agggcgctgg accggacgga cgcgcgggaa tctgctcgtc catgtggact
     2041 cgcacctgaa tctgctcggc cggaatgtac cggtccggat taccgaatcc tctccctggt
     2101 atttgattgg caccgttgac ggcgcggccg tctgactaac ttgcaaacct gctgacgacg
     2161 tcagcggaaa ggattctcga acgtgagtga tacgaagcaa gccacccggc ggcaactgat
     2221 cgaagatgcc cgcctggccg cgattgaagg ccgatggggc aatgccctga cgatcaacca
     2281 gcagttgatc gacaagaccc cgcgcgacgc cgccgcgttc aatcgagttg gcaaggcgca
     2341 actggagctg ggcaatgttc aggaggcaat cgacgcctac acctcgagtc tgaagatcga
     2401 cccggcgaac atgatcgcac gcc
//
LOCUS       DASSGX010000081         5847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_15376, whole genome shotgun sequence.
ACCESSION   DASSGX010000081 DASSGX010000000
VERSION     DASSGX010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5847
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15376"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VFI12_01405"
     CDS             complement(<1..210)
                     /locus_tag="VFI12_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055083.1"
                     /translation="MIEPPASTTGTLAQPPARRPMSAWSLFLVLILFYVVFRARIVVE
                     IGLLAFLYATIIERPVVRLERRGIPR"
     gene            520..2475
                     /locus_tag="VFI12_01410"
     CDS             520..2475
                     /locus_tag="VFI12_01410"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7055084.1"
                     /translation="MMSRVSTRVRFVLAMMLLLVPVIAIAAIILDESFKRSQEQIITS
                     EFATADVVSQSVSELIRGQQQVLGVLAETEAIRSLDKQKDQASAQMDEYRLSRQSVNG
                     LFLLKNDLTVVAQTGGIEISSLTGSGFKAAAEAAVQGGQPTVSDVILLPSGEVKVIAI
                     VVPVFANADASASEAPSGALGAFYSVERLIAGFQPPAGFSTGSNIAVALVSSAGSIVS
                     VPGGTSDPSQIFQDGPQLETAIKSALANQRTRATFKDPSGLSRVTVAVPIDMPGNDWA
                     VLVSAPETTSYGPNRMLIERAVIAAIGVVVLSFLLAILLAEWLARPFRLLTSQAKALS
                     TGAVVLDLEPVGPTDAATLSRTIREMADRLRAQIRDTEAAREEIARQAETLRDLLRRT
                     VRLQEDERRRIASDIHDAVSPLITGALYQAQAVRMARSGSNGNGHEGNGHHADQLAAA
                     KDDDSLQEVSELLERAMRELHDVIFDLRPPDLDDIGLEAAIQRHVDQVNRSGLPCTLE
                     VIGEERRLSPEVRLAIYRIVQEALHNSIRHARADESFVRIEWLPERLRVTVQDDGSGF
                     INESTERRAGLGLMSMRERAGSIGASLDIVSRPGTGTAIVVERMNDGLDLTADTPAAE
                     PQFDDQPEPGDSVEEDTADAGEEHALL"
     gene            2472..3107
                     /locus_tag="VFI12_01415"
     CDS             2472..3107
                     /locus_tag="VFI12_01415"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7055085.1"
                     /translation="MISVLLVDDHPVVVEGLRKLLTQTGEIVISAEANDAATGIKLAK
                     QIQPDVVLLDLRMPGATGIQAVRRMREQEVRSAVIILTSYGDQAYVRQAMEAGADGYL
                     LKSTPPDGLIQSIRNAARGRKPLSPELIDGVLADFGGLAREQTRRQADLTDEDREILR
                     RASEGATNKEIGFAMGRSEIAIKKRLQSIFAKLGAVDRAHAVAEAIRRGLI"
     gene            complement(3693..4811)
                     /locus_tag="VFI12_01420"
     CDS             complement(3693..4811)
                     /locus_tag="VFI12_01420"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET7055086.1"
                     /translation="MNRRERWVLTIGLTIGMLVTLMIAGTGLVSLGGGARAQDATATA
                     EPAADVQPFTSAVDVVKKVSPAVVTVVNEQRVGLQNGSSSLQPVGSGTGFIIDTDGHI
                     VTNWHVVDGGTQFQVVFADGTTKDAKLIGSDEISDLAVVQVSGTMPGTVPLGDSDAIE
                     VGEPVLAIGSPLGNFQNTVTEGIVSATNRDFPDGGQSQSQYNNLIQHDAAINPGNSGG
                     PLVNFKGEVIGVNTLGISVDQQGQPIQGLFFAIPSNSVKQVTDELIKNGSVTYPYFGI
                     RYQTNSAAAAAQLNLSTDQGVVVIEAPSGGPAADAGIEAGDVITKVGDYELDAQTTFS
                     EALFHYKPGDKIDVTFKRGDKDMTVTVTLGDRSKSLSQ"
     gene            4970..5728
                     /locus_tag="VFI12_01425"
     CDS             4970..5728
                     /locus_tag="VFI12_01425"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET7055087.1"
                     /translation="MTTAATDPMRELSRLFARTEVVDLSAVLEEGAPIWPTHPPLIIH
                     RTVTHERHGYFTNSIFMPEHIGTHCDAPAHAVPELMDQTIDRYPIDQMIGPAAVIDVS
                     DRNLQAGEMLTAADIRAWETEHSRIQPGEIVLINFGWLARHWRTDEQARYYVTNQPGL
                     DESAVKLLYERGIKALGSDNTNVETPIRDGVMLNPGYAHKKYFLPNGVPLIESLANLD
                     RLPPRCWFFALPLKIKGDSGSPIRPIALIPRVED"
BASE COUNT         1027 a         2122 c         1727 g          971 t
ORIGIN      
        1 cctcggaatc ccgcgccgct ccagccgtac gaccggccgc tcgatgatcg tcgcgtacag
       61 gaacgccagc agcccgatct cgacgacgat ccgggcccgg aacacgacgt agaagaggat
      121 caggacgagg aacaggctcc acgccgacat cggccgccgc gcgggcggct gggccaatgt
      181 gccggttgtg ctggcaggcg gctcgatcat ggcggcgccc cgggcaaggc cagccctcgc
      241 cccatcgcgg aaatcatacg cccggccgca ttcgccgatt tgacgcaggt ttacacaatt
      301 tgggacagat ggatactgcg gcgggcgcat acgtataggc gtggcctctc gagcatcacg
      361 cactattagc acggcgtcac agtctgcaga tgcacagggt atgctggatc gtgctggcat
      421 tgccgagggt gcccagcccg tgacgcagga gcacgtgaac acagctaatc tcgggcgttc
      481 cacactgcgt ctggcccttg ccgctggacg caccaaatca tgatgagtcg cgtttcgaca
      541 cgagtgcgct tcgtcctcgc gatgatgttg ctccttgtcc cggtcatcgc aattgccgcg
      601 atcatcctcg acgagagctt caagcgcagc caggaacaaa tcatcaccag cgagttcgcc
      661 actgcggacg tcgtctcgca gagcgtctcc gagttgatcc ggggacagca gcaggtgctc
      721 ggtgtccttg ccgagaccga agcgatccgc tccctcgaca agcaaaagga ccaggcgtcc
      781 gcccagatgg acgaataccg gctcagtcgc cagtcggtca acgggctctt cctgctcaag
      841 aatgacctca ccgtcgtcgc ccagaccggc ggcatcgaga tctcgtcgct gacgggatcg
      901 gggttcaagg ccgccgccga agccgccgtc cagggcggcc agccgaccgt gtccgacgtg
      961 atcctgctgc cctccgggga agtcaaggtc atcgcgatcg tcgtcccggt cttcgcgaat
     1021 gccgacgcgt cagcgtcgga agctccgtcg ggcgcgctcg gcgccttcta ctctgtcgag
     1081 cgcctgatcg ccgggttcca gccaccggcc ggcttctcca ccggttcgaa tatcgcggtc
     1141 gcgctggtca gctccgcggg cagcatcgtc agcgttcccg gcggcacgag cgacccgagc
     1201 cagatcttcc aggacggccc tcagctcgag acggcaatca aatccgcgct cgcgaaccag
     1261 cgcacccggg ccaccttcaa ggaccccagc ggcctcagcc gcgtcaccgt cgccgtcccg
     1321 atcgatatgc ccggcaatga ctgggctgtc ctcgtctccg ctccggaaac cacgtcctat
     1381 ggcccgaacc ggatgctgat cgagcgcgcc gtgatcgccg caatcggtgt cgtggtcctt
     1441 agcttcctgc tcgccattct gctcgccgag tggctggctc gcccgttccg cctccttacc
     1501 agccaggcca aggccctcag taccggagcg gtcgtgctcg acctcgaacc cgtcggcccg
     1561 accgatgccg ccaccctcag ccgcaccatc cgcgagatgg ccgatcgcct ccgcgcccag
     1621 atccgcgaca ccgaggccgc gcgcgaggag atcgcccgcc aggccgagac cctgcgcgat
     1681 ctgctccgcc gcaccgtgcg cctccaggaa gacgagcgcc gccgaatcgc ctccgacatc
     1741 cacgacgccg tctcgcccct catcaccggc gcgctttacc aggcccaggc cgtccgcatg
     1801 gcgcggtccg gcagcaacgg caatgggcac gagggcaacg gccatcacgc cgaccagctc
     1861 gccgcggcca aggatgacga cagcctgcag gaggtcagcg agctgctcga gcgggccatg
     1921 cgcgagctgc acgacgtcat cttcgatctg cgtccgcccg atctcgacga tatcggcctc
     1981 gaagccgcca tccagcgcca tgtcgaccag gtcaaccgta gcggcctgcc ctgcacgctc
     2041 gaagtgatcg gcgaggagcg tcgcctctcg cccgaggtcc gccttgccat ctaccggatc
     2101 gtccaggaag ccctccacaa ctccatccgc cacgcccgcg ccgacgaatc gttcgttcgc
     2161 atcgagtggc tgccggagcg gctccgcgtc actgtccagg atgacggctc cggcttcatc
     2221 aacgagagca ctgagcgccg cgcgggtctc ggtctgatga gcatgcgcga gcgggccggc
     2281 tcgatcggcg cctcgctcga catcgtctcc cgccccggca ccggcaccgc catcgtcgtc
     2341 gagcgcatga acgacgggct tgacctgacc gccgataccc ccgccgccga gccgcaattt
     2401 gacgatcagc ccgagccggg tgatagtgtc gaggaagata ctgccgacgc cggtgaggaa
     2461 cacgcgctcc tgtgatttcc gtcctgctcg ttgatgatca tccagtcgtg gtcgaaggtt
     2521 tgcgcaagtt gttgacgcag accggtgaga tcgtgatctc cgccgaggcc aatgacgccg
     2581 ccaccggcat caagctcgcc aagcaaatcc agcccgatgt cgtcctgctc gacctccgca
     2641 tgcccggcgc caccggcatc caggccgtcc gccgcatgcg cgagcaggaa gtccgctccg
     2701 ccgtcatcat cctgacctcg tacggcgacc aggcctacgt tcgccaggcg atggaggccg
     2761 gcgccgatgg ttacctgctc aagagcaccc cgccggacgg cctgatccag tccatccgca
     2821 acgccgcccg gggccgcaaa ccgctcagcc ccgagctgat cgacggcgtc ctggccgact
     2881 tcggcggtct tgcccgggag cagacccgcc gccaggccga cctgaccgac gaggatcgcg
     2941 aaatcctgcg ccgcgcctcc gagggcgcaa ccaacaagga aatcggcttc gccatgggtc
     3001 gcagcgagat cgccatcaag aagcgactcc agagcatctt cgccaaactg ggcgccgtcg
     3061 atcgcgccca cgccgtcgcc gaagccatcc gccgcggcct gatctaaccc aaccttcgcc
     3121 ccccaaccca actgaacgct cggcgccgcc cggggataga tgcccgtgct aaggaacgac
     3181 aagcccgcta aaggggctga caagcggctc ccgacaaatg tagccccggg cggcctcgcc
     3241 cggccgtgtc ccacggagcc cgctccacgg tgaccgagcc acctccaccg ccccggactg
     3301 aagaccgggg ctacgggtaa taaggcggct gatggtagtt gcgagcttga ttcggttgtc
     3361 cgtcgatacc gccccggatt gaaatccggc tctgatatcc ggatgtcggc tgaggccgac
     3421 cggtgcggag ccgtaccgag aagactcgtg tccccatgct ctgcgtcccg aagggacatc
     3481 cggaccctgg ctcgggaatt catttcggag cggcgcggga catccggttg ggcggtcgga
     3541 acccaaatgt cagccggctt cagccgggtt cagatttcag cccgggtgtt tatgcccggg
     3601 caaccgctag ccatcttccc gcgtccacaa aaaaacgggg gccggcccat gtcggaccgg
     3661 ccccggatct atgatgaacg cccgcggggc gattactggc tgagtgactt cgaccggtca
     3721 ccgagcgtga ccgtcaccgt catatccttg tcaccgcgct tgaatgtcac gtcgatcttg
     3781 tcgcccggct tgtagtggaa cagcgcctct gagaaggtgg tctgcgcgtc caactcgtaa
     3841 tcgccgacct tcgtgatcac gtcgcccgcc tcgatgcccg cgtcggccgc cggaccgccc
     3901 gagggcgcct cgatcacgac cacgccctgg tccgtcgaca ggttgagctg cgccgccgcc
     3961 gccgccgagt tggtctggta gcggatgccg aagtacgggt aggtcaccga gccgttcttg
     4021 atcagctcat ccgtcacctg cttgacacta ttcgacggaa tcgcgaagaa caagccctgg
     4081 atcggctggc cctgctgatc gaccgagatg ccgagcgtgt tcacgccgat cacctcgccc
     4141 ttgaagttga ccagcggtcc gcccgaattg cccgggttga tcgccgcgtc atgctggatc
     4201 aggttgttgt actggctctg gctctgcccg ccgtccggga agtcccggtt cgtcgcgctg
     4261 acaatgccct cggtcaccgt gttctggaag ttgcccagcg gggacccgat cgccagcacc
     4321 ggctcgccga cctcgatcgc atcggaatcg ccgagcggca ccgtgcccgg catcgtgccg
     4381 ctgacctgga ccaccgccag atcgctgatc tcgtccgacc cgatcagctt tgcgtccttg
     4441 gtcgtgccgt ccgcgaagac gacctggaac tgcgtgccgc catcgacgac atgccagttc
     4501 gtcacgatgt gcccgtccgt gtcgatgatg aacccggtcc cggatcccac cggctgcagc
     4561 gacgacgatc cgttctgcag gcccacccgc tgctcattga cgaccgtcac gactgccggg
     4621 ctcaccttct tgaccacgtc gaccgccgag gtgaacggtt gcacatctgc cgccggctcg
     4681 gccgtcgccg tcgcatcctg cgcccgggcg ccgccgccga gactgacgag gccggtcccc
     4741 gcgatcatca gtgtgacgag cattccgatg gtcagaccga tggtcagcac ccaccgctcg
     4801 cgtcgattca tgtcgaactg cctttctgta tgtacggcgg tgggacaatg ggagtcatgc
     4861 acgccggtga gcgtgccaat tcacacgctc cacaccacgt ttgacggata tccgccacca
     4921 tccgatagtg acgcccgatc atgaacggtg aatgaagagg tgcgacccaa tgaccactgc
     4981 cgctacggac ccgatgcgcg aactttcccg cctcttcgcg cgcacagaag tggttgatct
     5041 ctcggccgtc ctcgaagagg gcgcgccgat ctggccaacc catccgccat tgataatcca
     5101 tcgcaccgtg acccatgaac gccacggcta cttcactaac tcaatcttca tgcccgagca
     5161 cattggcacc cactgcgacg cccccgccca cgccgtcccc gagctgatgg accagaccat
     5221 cgaccggtac ccaatcgacc agatgatcgg ccccgccgcc gtcatcgacg tcagcgaccg
     5281 caacctgcag gccggtgaga tgctcactgc cgctgacatc agggcctggg agaccgagca
     5341 cagccggatt cagcccggcg agatcgtcct catcaacttc ggctggctgg cgcggcactg
     5401 gcggaccgac gagcaggccc gctactacgt caccaaccag cccggtctcg acgaatcggc
     5461 cgtcaagctc ctctacgagc gcggcatcaa ggctctcggc tccgacaaca ccaacgtcga
     5521 gacgcccatc cgcgacggcg tcatgctcaa ccccggctac gcccacaaga aatacttcct
     5581 gcccaacggc gtccccctca ttgaaagcct cgccaacctc gatcgcctcc cgccccgctg
     5641 ctggttcttc gccctccccc ttaagatcaa gggcgactcc ggatctccga tccgccccat
     5701 agccctgatt ccccgggttg aggattgagg gaacgcgagg ccgggaatac cttgccggcc
     5761 ccgcacgcgc gggccgaatg tcgccgcgtg atagccttcc gctcatcggt gctcgcgctc
     5821 cgtgggatgt gccattgctg aaccggt
//
LOCUS       DASSGX010000082         2427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_668094, whole genome shotgun sequence.
ACCESSION   DASSGX010000082 DASSGX010000000
VERSION     DASSGX010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668094"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VFI12_01430"
     CDS             complement(<1..394)
                     /locus_tag="VFI12_01430"
                     /inference="COORDINATES: protein motif:HMM:NF017856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HET7055088.1"
                     /translation="MRRLGLGERVLKTALAAGHSWQIAMLVPGNEHPYLAPISAVLLM
                     QLTIAQSIDLAIQRNIGIAIGVVVAVAAFAVFGVHAWSVGLVVLIALAAGIQLGLRQQ
                     AVQQIAVTALIVLLAGSVDGTFEYAVRRI"
     gene            complement(472..1554)
                     /locus_tag="VFI12_01435"
     CDS             complement(472..1554)
                     /locus_tag="VFI12_01435"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET7055089.1"
                     /translation="MISVNRLDAAFELLERFVGEGVPGAGLVVAERGEIVGEHYTGMA
                     RPDRPAAADTLWPLASISKLYAAAACMAVIEEGRIALGTRVATIFPEFTQGGRDKITL
                     RHLLTHTSGLPYESEQMADRLAAHLSLEELLRESYEGDLLFAPGTNQAYSDYGIGLAG
                     LMCATVMGMSFPDLVRSRVIDPGHLLDTFMPPPESEYHRIAHIEGPLAEGTDGAMYNS
                     AHARNLAHPAFGTIASARDLLRFGLFFDPNREPTLFSRVATRVMITDQTGYDFPDAVT
                     EPMSPAVRAWGIGFMLKGRMGFPELVSPATFGHPGATGCMVLVDPTYDVAFAFVSNLH
                     LNTGYDAWTDRLMMITNVVMASMTRG"
     gene            1830..>2427
                     /locus_tag="VFI12_01440"
     CDS             1830..>2427
                     /locus_tag="VFI12_01440"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HET7055090.1"
                     /translation="MTDLFAYTDEMRRLMDRAVAHAEARISHEVEPLQPPPDASVLNQ
                     ALAGAVTAEGIGPDAAFDLFTDVIAPNCLADDNPRFLAFVAHPPSTAAQIMDMALSAS
                     SIFGTSWLEAAGATAAENQALRWLADLAGMPASAGGVFLSGATVANFNALAAARACWR
                     ARNPGNDRRLGVAMTAEAHASAKMVARVLDFEIVPVEVD"
BASE COUNT          405 a          763 c          884 g          375 t
ORIGIN      
        1 caatccgccg gaccgcgtac tcgaaggtgc cgtcgacgct gccggccagc aggacgatta
       61 gcgccgtgac cgcgatttgc tggacggcct gttgccggag gccgagctgg atcccggcag
      121 cgagggcgat cagcacgacg agtccgaccg accaggcatg cacgccgaaa acggcgaagg
      181 cggcgaccgc gaccacgacg ccgatcgcga tgccgatgtt gcgctggatg gcgaggtcga
      241 tcgactgcgc gatcgtgagc tgcatcagca ggacggcgct gatcggcgcg aggtaggggt
      301 gctcgttccc cggtacgagc atcgcgatct gccaggaatg gcccgccgcc agcgccgtct
      361 tgaggacgcg ctcgcccagc ccaagccggc gcatgtggat gatgagctgc gacaggagca
      421 gcccgttctc gggatccggc cggggagcag ggggaatccg gttcagtggc gttaccctct
      481 ggtcatgctt gccatgacaa cgttcgtgat catcatcagc cggtcagtcc aggcgtcgta
      541 gccggtattg agatgcaagt tggagacgaa ggcgaaggcg acgtcgtagg tcggatcgac
      601 cagaaccatg cagccggtcg cgccgggatg gccgaatgtg gcgggggaga ccagctccgg
      661 aaagcccatc cgccccttga gcatgaaccc gatgccccag gcgcggacgg cgggcgacat
      721 tggctcggtg acggcgtcgg gaaagtcata cccggtctgg tcggtgatca tgacccgggt
      781 ggctacacgc gagaagagcg taggctcccg gttcgggtcg aagaacaggc cgaagcggag
      841 caggtcccgc gcgctggcga tcgtcccgaa ggcgggatga gcgaggttgc gggcatgggc
      901 ggagttgtac atcgcgccgt cggtaccctc cgcgagcggc ccctcgatgt gggcgatccg
      961 gtggtattcg ctttcgggcg ggggcatgaa ggtgtcgagc aggtggccgg gatcgatcac
     1021 gcggctgcgg acgaggtcgg ggaacgacat gcccatcacc gtcgcgcaca tcaggccggc
     1081 caggccgatc ccgtagtcgc tgtaggcctg gttcgtgccc ggcgcgaaga gcaggtcgcc
     1141 ctcgtacgac tcgcgcagca gctcttcgag ggagaggtgc gcggcgaggc ggtcggccat
     1201 ctgctccgac tcatagggta gaccggaggt gtgcgtcagc aggtggcgga gggtgatctt
     1261 gtcccggccg ccctgcgtga attcgggaaa gatcgtcgcg acccgggtgc cgagcgcgat
     1321 ccggccctcc tcgatgactg ccatgcaggc cgccgcggcg tacagtttcg agatcgaggc
     1381 gagcggccag agcgtgtcgg cggcggccgg gcgatccggc cgggccatgc cggtgtagtg
     1441 ctcgccgaca atctcgcccc gctcggcgac tacgagaccc gcgcccggga ccccttcgcc
     1501 cacgaaccgc tccagcagct cgaaggccgc atccaaccga ttgaccgaaa tcattgcccc
     1561 gcccatcacg ccagttcgat agcagcgcaa ttatcgatga gcggcgcggc aatgcgagcg
     1621 gcttctccgc gtggacggca ggcaacggcg tgattaggat tcggcagtac ggcgattcat
     1681 aatgggttgt tgccgcatcg ttccagatac cgcacggccc agtcggtttc aaccgacttc
     1741 cgttggtgag acgggggttt caacccccgg cggcgcggcg tatccggatc aacacggcaa
     1801 ccatcagatc gaatgacggc ggagcgcgga tgacggacct gtttgcgtac acggacgaaa
     1861 tgcggcggct gatggaccgg gccgttgccc atgccgaggc ccggatcagc cacgaggtcg
     1921 agccgctcca gccgccgccg gacgcgtccg tgctgaacca ggcgctcgcg ggggcagtca
     1981 cggccgaggg gatcggcccg gacgcggcgt tcgacctctt caccgacgtc atcgcgccga
     2041 actgcctggc ggacgacaat ccgcgctttc tggccttcgt cgcgcatccg ccctcgacgg
     2101 ctgcccagat catggacatg gcgctctcgg cctcgtcgat cttcggcacg tcgtggctgg
     2161 aggcggccgg ggcgaccgcc gccgagaacc aggcgctgcg ctggctggcc gatttggccg
     2221 ggatgccggc gagcgcgggc ggcgttttcc tgagtggggc gacggtcgcc aacttcaatg
     2281 cgctggcggc ggcacgggcc tgctggcggg cgaggaatcc cgggaacgat cgccggctcg
     2341 gggtcgcgat gaccgccgag gcgcacgcgt cggcgaagat ggtcgcccgg gtgctggatt
     2401 tcgagatcgt gccggtggaa gtggacg
//
LOCUS       DASSGX010000083         3862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1211273, whole genome shotgun sequence.
ACCESSION   DASSGX010000083 DASSGX010000000
VERSION     DASSGX010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211273"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VFI12_01445"
     CDS             <1..283
                     /locus_tag="VFI12_01445"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET7055091.1"
                     /translation="EWVTITVTDEGIGIAPEHVDTVFERFRRVGGDPRVRGMGLGLYL
                     SRHLVEAQGGLISVTSPGPGLGASFSVTLLMAREWADGDDGGNADQGEA"
     gene            280..1023
                     /locus_tag="VFI12_01450"
     CDS             280..1023
                     /locus_tag="VFI12_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7055092.1"
                     /translation="MRKHVVLVVDDEPAIVRLVRAKMQADGRAVISANSGEEALKVLE
                     DERPDLIVLDVMMPGIDGFETLRRIRTGAFSSQVPVIMLTARASDADRLKGLNAGADD
                     YMSKPFNPDELAARVTAVLRRTAGAVTTAGHGPLRYPGEPPVEIDLDRRRVLVGEEEA
                     KLSRTEWELLTQLAANAGRVMMHGELLSRIWGPEFRDEVHYLRTWVSRLRAKLEPGPE
                     SPTLITTFPGLGYRLEAPPSDLGHVSADQ"
     gene            1126..1791
                     /locus_tag="VFI12_01455"
     CDS             1126..1791
                     /locus_tag="VFI12_01455"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7055093.1"
                     /translation="MLVDHPVAGVDSFIPAGSGWSSVVRSRKQSPQLNVPDHIVNSST
                     NGIAGPNDMIVAPLHPKHSVRSVGGNTSIASGKLPHQSEGDVNMRLRTVSPDAPRVAR
                     ILIVARPALSRALSSTLAAAGYEVHRTPDPASAVEAARRLRPQLAIVAKEVPGSTGMK
                     VAHLLHASQSTLPVILLGGASGEPDAPAIVAVPTDVTPAALLTEIAQQIPQTSEEDDP
                     NAG"
     gene            1781..3100
                     /locus_tag="VFI12_01460"
     CDS             1781..3100
                     /locus_tag="VFI12_01460"
                     /inference="COORDINATES: protein motif:HMM:NF014594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HET7055094.1"
                     /translation="MPAEAPHTSRALQFVSADQAVELVRSGDRVYLHEAAMAPLELME
                     ALVRRAPELRDVETVSIHTEGPAPHVAPGLEGHIRHNALFVGSNVRQAVNEGRADYTP
                     VFLSDIPALFHGTDLPLDVAFVQVSPPDRHGFCRLGVSVSVARAAVDNARTVIGLVNP
                     RVPLTLGNSAVHISRFAALVETDRPLPTLAPGPISEISEQIGQHVASLIPNRATLQLG
                     IGAIPDAVLSCLTNHEDLGIHTEMFSDGLLALAERGVITNRYKATWRGRVVTSFALGS
                     QELYNFIDGNPFVEFHSSDIVNDTREIRRNPNVVAINSALEIDLTGQVVADSIGEYIY
                     SGIGGQMDFVRGARLSPGGKAVISLPSTAKGGAISRIVPTIKPGSGVVTTRGHVEYVA
                     TEYGVVDLAGQSLRRRAELLISIAHPDTRADLRAAAQNRNYFIPAHA"
     gene            3229..3738
                     /locus_tag="VFI12_01465"
     CDS             3229..3738
                     /locus_tag="VFI12_01465"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7055095.1"
                     /translation="MFTRIVVPLDGSVLAESALPKAAELTRLTGAELTLVRVIDFMTL
                     EKSGAYGLALEYAPPREILDVEMEEADSYLRDVSAKLRASGFAVSTEILRGRVARVLA
                     DFGQPGDVVVMASHGRGGLSRWFLGSVAEDLVRRSTVPVMLIRAQQPPKQAEPGTVVV
                     PASTSAVLV"
BASE COUNT          655 a         1266 c         1246 g          695 t
ORIGIN      
        1 cgagtgggtc acgatcacgg tgactgacga gggcatcggg atcgcgccgg aacatgtcga
       61 cactgtcttc gagcggtttc gacgggtcgg gggcgatcct cgcgtgcggg gcatgggcct
      121 cgggctctac ctcagccgcc acctcgtcga ggcgcagggc gggctcatct cggtcacctc
      181 gcccgggcct ggcctcggag cgagcttctc ggtgacgttg ctgatggcca gggaatgggc
      241 ggatggcgat gatggcggta acgccgacca gggtgaggca tgagaaagca cgtcgtactc
      301 gtggtagacg atgaaccggc gatcgtgcgg ctggtccggg ccaagatgca ggcggacggc
      361 cgcgcggtga tttcggccaa cagtggcgaa gaagcgctca aggtactgga agatgagcgg
      421 ccggatctga tcgtcctcga tgtgatgatg cccggcatcg acggattcga gacgctgcgc
      481 cggatccgta ccggtgcatt ttccagccag gtgccggtaa tcatgctgac cgcgcgcgcc
      541 agcgatgccg atcgcctcaa agggctcaat gcaggcgcgg acgattacat gtccaagccg
      601 ttcaacccgg acgagctcgc cgcccgtgtc acggcggtgc ttcgtcgcac ggcgggggcc
      661 gtcacaaccg ccggtcacgg ccccttgcgc tacccgggcg agccgccggt cgagatcgat
      721 ctcgaccggc gccgcgtgct cgtcggcgag gaagaggcca agctcagccg cactgaatgg
      781 gagcttctga cccagttggc cgcgaacgcc ggccgcgtga tgatgcacgg tgaattgcta
      841 tcccgcatct ggggacccga gttccgggac gaggtgcatt acctgcggac ctgggtgagc
      901 cggcttcgcg caaagctcga gccagggccg gaatcgccaa cactgatcac cacattcccg
      961 ggcctgggct atcgcctgga agctcctcct tccgacctgg gtcacgtctc cgccgatcaa
     1021 tagcagcctg cgcaaatctg acacggattt gttatgccgc cgtccctcgt gcggcctccc
     1081 tgtcatgcct cgggtcctat cgtagagcca tgcaaccgga actcgatgtt ggttgatcat
     1141 ccggtggcag gcgtcgacag tttcattccc gccgggtcag gctggagttc agtcgtgaga
     1201 tctcgaaaac agagcccaca actgaacgtg cccgaccata tcgtgaattc gtcgacgaat
     1261 ggcatcgcag gacctaatga catgatcgtt gcaccactcc atccaaagca ttcggtccga
     1321 tcggtcggag gtaacacgag cattgcatcg ggaaagctcc cacatcaatc ggaaggcgac
     1381 gtgaatatgc gcctcaggac ggtgagtccg gatgcgcccc gcgtggcccg gatcctgatc
     1441 gtggcgaggc ccgccctcag ccgggcgctg tcctcgacgc tggccgcggc cggctacgaa
     1501 gtgcaccgca cgcccgatcc ggcaagcgcg gttgaggctg cgcggagatt gcgaccgcag
     1561 ttggcgatcg ttgccaagga agtccccggt tcgaccggca tgaaggtcgc gcaccttctg
     1621 cacgccagcc agtcgacctt gcctgttatc ctgcttggcg gcgcttctgg ggagcccgat
     1681 gcgcctgcga tcgtcgcagt cccgaccgac gtcacgccgg ccgcgctgct cacggaaatt
     1741 gcgcagcaaa tcccgcaaac gtctgaggaa gatgacccga atgccggctg aggcgcccca
     1801 cacgtcacgc gcactccaat tcgtaagcgc agatcaggcc gtggagctcg tacgatcagg
     1861 cgatcgcgtt tacctgcacg aagctgcgat ggctccgctc gagttgatgg aggcattggt
     1921 ccgccgcgct ccggagttgc gggacgtcga aaccgtctcg attcacaccg aggggccagc
     1981 gccgcacgtc gctccgggac tcgagggcca tattcggcac aacgcgctct tcgttggctc
     2041 caatgtgcgc caggccgtga atgaggggcg ggccgactac acgccggtgt tcctctcgga
     2101 cattccggcg ctgttccacg ggaccgactt gccgcttgat gtcgccttcg tccaggtttc
     2161 accgcccgac cggcatggct tctgccgtct cggcgtctcc gtgtccgtgg cacgcgcggc
     2221 agtggacaac gcgcggacgg tcatcgggct ggtcaacccg cgcgtgccgc tcacgctcgg
     2281 gaattccgcg gtccacatca gccggttcgc ggctctggtc gaaaccgatc ggcccttgcc
     2341 gaccctggcc cccggcccga tcagcgagat ctccgagcaa attggacagc acgtcgcctc
     2401 gctgatcccg aaccgggcga cgctccagct cggcatcggc gccattcccg acgccgtcct
     2461 ctcctgcctg acgaaccacg aggacctcgg gatccatacc gagatgttct ccgacggctt
     2521 gctcgcgctg gccgagcgtg gcgtgatcac gaaccgctac aaggcaacct ggcgcggccg
     2581 tgtcgtcacc tcgttcgcgt taggcagcca ggaactctac aacttcatcg acggcaaccc
     2641 gttcgtcgag ttccactcct ccgacatcgt caacgacaca cgcgagatcc ggcgcaaccc
     2701 gaatgtggtc gcgatcaact ccgcgctcga gatcgacctg accggccagg tcgtggccga
     2761 ctcgatcggc gaatatatct acagtggtat cggcgggcag atggacttcg tccgcggcgc
     2821 gcgcctctcc cccggcggca aggcggtcat cagccttccg tcgacggcga aaggcggcgc
     2881 aatctcgcgg atcgtaccga cgatcaagcc cggctccggc gtcgtcacca ctcgcgggca
     2941 tgtcgagtac gtcgcgacgg agtatggcgt tgtcgatctg gccgggcaga gcctgcgccg
     3001 ccgcgccgag ctgctgatct cgatcgcgca ccccgatacc cgcgccgacc tgcgcgcagc
     3061 cgcccagaac cgcaactact tcattccggc gcacgcctga caccagcgca cagacgcatg
     3121 ttgcgatttt gtgatgccca tgccggcact gtgatcgcct ctgtcatgcg cggagatgca
     3181 cgataggcac aggattgatc tgtgcatctc acagttgcgg aggatctcat gtttacccga
     3241 atcgttgttc ccctcgacgg ctccgttttg gccgaatcgg cgcttcccaa ggcggccgaa
     3301 ctgacgaggc tcaccggcgc cgagctaacg ctcgtgcgcg tgatcgactt catgaccctg
     3361 gagaaatccg gcgcgtatgg gttggcgctt gaatatgccc cgccgcggga gatcctcgac
     3421 gttgagatgg aggaagccga ttcttacctg cgcgacgtgt ccgcgaagct gcgcgcgtcc
     3481 ggtttcgcgg tgtcgaccga aatcttgcgt ggacgggtcg cgcgcgtact ggccgatttc
     3541 ggccaaccgg gcgatgtggt cgtgatggcg tcgcacgggc ggggcgggtt gtcccgctgg
     3601 ttcctgggca gcgtcgcgga ggacctggtt cggcggtcga cggtgccggt catgttgatc
     3661 cgcgcccagc agcccccgaa gcaggcggag ccgggcacag tcgtcgtgcc ggcctcgacc
     3721 tcagcggtgc tggtctagcc tattcctcca cctgcctcga acatgagcgg aggtgcgatc
     3781 gtgaatctgg ttgtaggcga ccaagccagc cggcgggcca gccggtcggt tgcatggcgg
     3841 gaccagttcg ttgcgtggac ct
//
LOCUS       DASSGX010000084         3981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1321391, whole genome shotgun sequence.
ACCESSION   DASSGX010000084 DASSGX010000000
VERSION     DASSGX010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1321391"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..1895)
                     /locus_tag="VFI12_01470"
     CDS             complement(63..1895)
                     /locus_tag="VFI12_01470"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HET7055096.1"
                     /translation="MATTARRQTETRYTFEQFTAIRRYQPTLSFSPDGREIAYVVNTS
                     GQYNIWRQSSEGGFPYQLTLFTDQTPREIAWSPDGQTIAFAADRDGDEYKQIYTIPAR
                     GGRVSAQTEGPRVRHELAPEAWSPDGTSLLIAGNDRSETDQDVMIRDLATGETRRVVD
                     YGGMYFPISWAPDGKSVTVIDFKSNTNMDLYVLTVDNEEMQHLTPHEGEIQYLPGPWA
                     ADGSGFWVLTDEGREFTGLAFWDLKAGMRWVDTPDADVENLALSGDGRYLAWIVNQEG
                     YSTLKVRDLRSNQDMALPDMPKGVIEALAMSRSGGMIGFLLAEATHPFEVYVLDLEHS
                     RLTRLTESMLGGIDEADLTAPELIHYPTHDGREIPAWLYRPEGEGPFPVILSIHGGPE
                     WQERPTYNYAGFYQYLLTRGIGIMAPNVRGSTGYGKSYQKLIHHDWGGAELGDFKAAA
                     DYLKGLDWVDGDRLGVYGGSFGGFATLSCVTRLPEYWAAAVDIVGPSNLVTFAKAVPP
                     TWRRFMDQWVGNPETEVDFLMERSPITYVDQVTAPLFVIQGANDPRVVKGESDQIVER
                     LRARGVEVKYDVYEDEGHGFTRRVNELKAMSDTAAFFEQYLLES"
     gene            complement(2334..>3981)
                     /locus_tag="VFI12_01475"
     CDS             complement(2334..>3981)
                     /locus_tag="VFI12_01475"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET7055097.1"
                     /translation="RDIPDVQPSKRSKKALLWGSLMLLGGALLAMSGHGAEKAFPFYA
                     GMSLLPFGVALFARFFGAPSRPVFSAVGAWLLFLWLLPEGPSEKLWGNLDGGMEMFFL
                     SGIFMVVGATVLLVQNLDVLLAGVSRLGRLFKSQTPAVRTAIAYPGASRGRTGMAIAM
                     FSLIIFSLVTVSTMNENYTALFLGDEAAAGWDVRVDATNANPMGDFRQALDQNGVDTS
                     AFTEIGTVTTPAPFASRVRMAGTDDWKSFEVLGMDGSFIQASTLTFGQRAEGYESDRA
                     IIEALLTQPNVAVVDVSALGGNGDIGGEADRFQLNGVTADDKTFAPATVDVINPATGQ
                     PATLTIIGVIDSKIGTLSGLYANQATIDTIYPTAVMTSYYVALTDDSQAEAVAKQIES
                     TMLSYGVQAASIDDELAEGQKQSANFLHIVEGFMGLGLIVGIAAVGVISFRSVVERRQ
                     QIGVLRAIGFQRRMVSLSFLIETGFIVGMGVISGTVLGLALARSLVTSDEAANGSQFL
                     VPWPMISVILIVTLAVALLMAWIPSRQAARIAPAEALRYE"
BASE COUNT          696 a         1368 c         1216 g          701 t
ORIGIN      
        1 gaacctcacc cgggcaattg cgttcagatc ctcgccgctc gtctcaactc acgactcacg
       61 tctcacgact ccagcaggta ctgctcaaag aatgcggccg tgtcgctcat ggccttgagc
      121 tcattgacgc gccgggtgaa gccgtgacct tcgtcctcgt agacgtcgta cttgacctcg
      181 acgccgcgcg cccgcagtcg ctcgacgatc tggtcactct cgcctttgac gacgcgcggg
      241 tcgttcgcgc cctggatcac gaagagcggc gccgtgacct gatcgacgta ggtgatcggc
      301 gagcgctcca tcaggaagtc gacttcggtc tcgggattcc cgacccactg atccatgaac
      361 cggcgccagg tcggcgggac tgccttggcg aaggtcacca ggttcgacgg cccgacgatg
      421 tccaccgccg ctgcccagta ttcgggtagc cgggtcacgc aggaaagggt cgcgaagccg
      481 ccgaacgacc cgccgtagac gccgagccgg tcgccgtcga cccagtcgag ccccttcagg
      541 tagtcggcgg ccgccttgaa atcgcccagc tccgctccgc cccagtcatg gtggatcagc
      601 ttctggtagc tcttgccgta gccggtcgag ccgcgcacgt tcggcgccat aatgccgatc
      661 ccccgtgtca gcaggtattg gtagaaaccg gcgtagttgt acgtcggccg ttcctgccat
      721 tccgggccgc catgaatcga gaggatgacc ggaaacggac cctccccctc gggccggtac
      781 aaccaggccg ggatctcgcg cccatcatgc gtcgggtagt ggatcaactc gggcgcggtc
      841 aggtccgcct cgtcgatgcc gccgagcatg ctttccgtca atcgcgtcag ccggctgtgt
      901 tcgagatcca gcacgtagac ctcgaacgga tgcgtcgcct ccgccagcag gaacccgatc
      961 atgccgcccg agcgcgacat cgccagggcc tcaatcacgc ccttgggcat atccggcagc
     1021 gccatgtcct ggttcgagcg caggtcccgg accttgagcg ttgaatagcc ttcctggttg
     1081 acgatccacg ccaggtagcg tccgtcgccc gagagcgcga ggttctcgac gtcggcatcc
     1141 ggcgtgtcga cccagcgcat gcccgccttc aggtcccaga aggcaagacc ggtgaactcg
     1201 cgtccttcgt ccgtcagcac ccagaagccg gagccatccg ctgcccacgg accgggcagg
     1261 tactggatct cgccctcatg cggggtcagg tgctgcattt cctcgttgtc gacagtcagg
     1321 acgtagaggt ccatgttcgt gttcgacttg aagtcgatca ccgtcaccga tttcccgtcc
     1381 ggggcccacg agatcgggaa gtacattccg ccgtagtcga cgacccggcg tgtctcgccg
     1441 gtcgccaggt cgcggatcat cacatcctga tccgtctcac tgcggtcgtt gccggcgatc
     1501 aggagcgagg ttccatccgg cgaccatgcc tccggggcga gctcgtgacg aacgcgaggc
     1561 ccctccgtct gcgccgaaac tcgcccgccc cgggctggaa tcgtgtagat ctgcttgtac
     1621 tcgtcaccgt cgcgatcggc cgcgaatgcg atcgtctgcc cgtccggcga ccacgcgatc
     1681 tcgcgcggag tctggtcggt gaagagcgtc aactggtacg gaaacccgcc ctcgctgctc
     1741 tgccgccaga tgttgtattg gccggaggtg ttgaccacgt acgcgatctc tcgcccgtcc
     1801 ggggagaagg agagggtcgg ctggtagcgc cggatcgccg tgaactgctc gaaggtatag
     1861 cgcgtttcgg tttgacgccg cgcggtggtt gccattgcct gctcctctat gaatcggttc
     1921 aacacatgcc acattccagc atacgcgcgc cggaacgcgg tacatcactc ctaagaggta
     1981 ttcgcaacac ccgcccccaa acggaggtac ggcgcccaaa cgtagcgcgg tcccccgtgg
     2041 gccacccgga ccacgtcccg acgcaagcgt tggcgggaac caacctcagc cccggatcga
     2101 ctccatctag cgcgcgtggg gatcatgatg cggcctacgg gggaccgcgc tacggtgcga
     2161 tccaccagcc ggcttcggcc gggctcaggt ttcagccccg gtgtttacgc ccgggcgaca
     2221 ccgcgcgcga acagggcggg ggccaggcaa ccttccggcc acccgacccc cgacccatga
     2281 gcccgttcgg ctcacggctc acggctcacg tctcacgcct cacgtctcac gtctcactcg
     2341 taccgcagcg cctccgccgg ggcgatccgc gccgcctggc gggaaggaat ccacgccatc
     2401 agcagggcca ccgccaacgt cacgatcagg atcactgaga tcatcggcca cggcaccagg
     2461 aactgggagc cgttcgccgc ttcatcgctc gtgaccaggc tccgcgccaa ggccagccca
     2521 agcactgtgc cgctgatcac acccatcccg acgatgaacc cggtttcgat caggaagctg
     2581 agtgagacca tccgccgctg gaagccgatt gcgcgcagga cgccgatctg ctgccgtcgc
     2641 tcgacgacgg atcggaagga gattacgccc accgcggcga tcccgacgat caggccgagg
     2701 cccatgaaac cctcgacgat gtgcaggaag ttggccgact gcttctggcc ctccgccagt
     2761 tcatcgtcga tcgaggccgc ctgtacgccg tagctgagca tggtcgattc gatctgcttc
     2821 gccactgcct cggcctggct gtcgtcagtc agggcgacgt agtacgacgt catgacggcc
     2881 gttgggtaga tcgtgtcgat cgtcgcctgg ttggcgtaca gaccgctcaa tgtcccgatc
     2941 ttgctatcaa tcacgccgat gatggtcaga gtcgctggtt gtccagtcgc cggattaatc
     3001 acatcgaccg ttgctggcgc gaatgtcttg tcgtcggcgg tgacgccgtt caactggaag
     3061 cggtcggcct cgccaccgat gtcaccgttg ccgccgaggg ccgatacatc gacgaccgcc
     3121 acgttcggct gggtcaggag cgcttcaatg attgcgcggt cgctttcgta gccctctgcg
     3181 cgctgcccaa aggtcagggt gctggcctgg atgaacgaac cgtccatgcc gagcacttcg
     3241 aacgacttcc aatcgtccgt tcccgccatc cgcacccgtg acgcgaacgg tgccggagtc
     3301 gtcactgtgc cgatttccgt gaaggcgctg gtatctaccc cgttttggtc gagcgcctgg
     3361 cggaaatcgc ccatcgggtt cgcattggtc gcatctaccc ggacatccca accggccgcc
     3421 gcctcgtcgc cgaggaagag cgccgtgtag ttctcgttca tggtcgacac cgttaccagc
     3481 gagaagatga tcaggctgaa catggcgatc gccatgcccg tgcggccccg ggacgcgccc
     3541 ggataggcga tagcggtgcg gactgccggg gtctgcgact tgaagagcct gcccagccgg
     3601 gagacgcctg cgagcaggac atccagattc tggaccagca gcaccgtggc accgaccacc
     3661 atgaagatgc cggacaggaa gaacatctcc atgccaccgt caaggttgcc ccagagcttc
     3721 tcgctcggcc cctccggcag gagccacagg aagagcagcc atgcacccac cgcgctgaaa
     3781 accggccgtg atggcgcgcc gaagaaccgg gcgaacaggg cgacgccgaa cggcagcaga
     3841 ctcatcccgg cgtagaaggg gaacgccttc tccgcgccgt ggccgctcat ggccagcagt
     3901 gcgccgccga gcagcatcag actgccccag agcagcgcct tcttgctccg cttgctcggc
     3961 tgcacatcag gtatatcgcg g
//
LOCUS       DASSGX010000085         5279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_450483, whole genome shotgun sequence.
ACCESSION   DASSGX010000085 DASSGX010000000
VERSION     DASSGX010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5279
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450483"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VFI12_01480"
     CDS             <1..500
                     /locus_tag="VFI12_01480"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7055098.1"
                     /translation="LPIMVVLSLIYTIPSLAFLAFLIPSLHLGRRPAIVVLAAYAQLA
                     LVRNIAAALRGVDPATIEAARGAGMTWRQVLWRVRLPLALPVLIAGLRIATVTTIGLA
                     TITAWINAGGLGSLLFDGISRDYPSQILAGTVAIVALSLLIDLVLRVAEWLTPAARAR
                     RSVAG"
     gene            564..1493
                     /locus_tag="VFI12_01485"
     CDS             564..1493
                     /locus_tag="VFI12_01485"
                     /inference="COORDINATES: protein motif:HMM:NF039315.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HET7055099.1"
                     /translation="MRSDSWPALPLAEWQETLDTLHMWTQIVGKVKLALTPFLNEWWN
                     VALHVNARGLTTGAIPAGAGIFDVEFDFVDHILVIRTSAGERRELALEPRSVANFYAA
                     FLATLAALGIEVTIDPIPAELPNPTPFDVDEIHAAYDAAAVARFWQALIDIQRVLMRF
                     RSEFFGKSSPVLFYWGSFDLAHARFSGRPIDPPVGMPRFFQIAEDQENFACGWWPGNV
                     SAAGITFGEPAFYAYFVPSPEGLRTAPIQPAGAGWDTTMGEFIYRYGDARQAADPAEA
                     VLAFFRSVYEAGADLANWDRAVLERDIRKELVS"
     gene            1490..1747
                     /locus_tag="VFI12_01490"
     CDS             1490..1747
                     /locus_tag="VFI12_01490"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HET7055100.1"
                     /translation="MSQQCSHLDQIRDVEPSARGCEECLAMGDTWVHLRECLICGHVG
                     CCDSSKNKHATKHFHATGHPIVQSFEPGEDWRWCYVDNMFV"
     gene            complement(2054..3028)
                     /locus_tag="VFI12_01495"
     CDS             complement(2054..3028)
                     /locus_tag="VFI12_01495"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HET7055101.1"
                     /translation="MVTRRTIIKGFAAAPVLGTGLAVGPAAFAQGSTIKVGSKDFPES
                     IIIGEMYALLLEHAGLTVERKLNLGGTAVAQEALKNGDLDVYPEYTGTGLLVVLGKTL
                     ADVPAATPEAAASPMAGATPAAGGMVEAVYDYVKAQYKEQFNLVWLDQSPMNDSQALA
                     VTRKFSEENNVTTISQLAALSSSVDLTISAPSDFEERDDGLKGLKATYGDFKASVNGV
                     APGIKYKALIDGDANVVLAFSTDAEIAINDLVVLEDDKGLWPPYHVAPVVRQEALDAN
                     AGLAPALNAVAPVLTTEKMTAMNARVVGDEKAEPATVAKDFLTEQGLI"
     gene            complement(3074..3748)
                     /locus_tag="VFI12_01500"
     CDS             complement(3074..3748)
                     /locus_tag="VFI12_01500"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7055102.1"
                     /translation="MHLLRDSLQYIQDNPDRFRAALSTHIQLSLYALLAAIAIFVPLG
                     ILAARSRRIGPSLIGLVAAARVVPSISVLFLLYPYRKPIGDLLPFWPRAFVLALIALI
                     LLAGPPLLINTDAGLRAVSPAVLENARGLGMSELQVLRKVQLPLALPVIIAGVRTAAV
                     EVVASATLAAFVGAGSLGRFITSGLTLLDYPLLFVGAVPIALLALVVEVSLAGLERLV
                     TPPAAP"
     gene            complement(3855..>5279)
                     /locus_tag="VFI12_01505"
     CDS             complement(3855..>5279)
                     /locus_tag="VFI12_01505"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET7055103.1"
                     /translation="EAVTSDDREIDTLAGLTFLVESSLLRQVDGREGAARFQMLETIR
                     EYGQEQLAAEGVDAATRDVHAAFFLALAERSTWVWTSSTGHWFAVVSAEIDNLRAALG
                     RFEETGDAVSQLRIVAALGWFWHHSGRYREGRDRLERILAGAPDIPSPLRTLAFDWTG
                     WLSLMLGDLDRARQYAEQTLPAARDDGDPLALCMALLLCAGIDSECGDDTAAISYLEE
                     TVAVGQAGGVDFAARAALNNLGLLAIPREDFARARDYLERAHALNQQADDVLSLAQST
                     GNLGLVLQRVGEVHRAAQLCREQVTLYLERGLDFGLDGPALFVAEAGNPELAARLYGS
                     DEAVAERLGRTPYQDNSFRTTYEQSVASIRAALSPNAFAAAWAAGRAMPRDQVFAESL
                     AFLTPFTERESAPTAPTPAFGLSPRELEVLRLVAQGKSNGEIAAALFISVPTAKVHVR
                     SILTKLALDSRTAAAAFAIRNGLA"
BASE COUNT          799 a         1644 c         1973 g          863 t
ORIGIN      
        1 cgctgccgat catggtcgtg ctgagcctga tctacacgat cccgagcctg gccttcctcg
       61 ccttcctgat cccgagcctg cacctcgggc gacggccggc gatcgtggtg ctggcggcct
      121 acgcgcagct cgcgctggtc cgcaacatcg cggcggcctt gcggggggtc gatccggcca
      181 cgatcgaggc ggcccggggc gcggggatga cgtggcggca ggtgctctgg cgggtgcggc
      241 tgccgctggc gctgccggtg ctgatcgcgg ggttgcggat cgcgacggtg acgacgatcg
      301 gcctggcgac gatcacggcc tggatcaatg ccggcgggct gggctcgctg ctgttcgatg
      361 gcatctcgcg cgattacccg tcgcagatcc tggccgggac ggtcgcgatc gtggcgctct
      421 cgctgctgat cgatctggtg ctgcgcgtgg cggagtggct gacgccggcg gcgcgggccc
      481 gacgcagcgt agccgggtga cagcgggcgc ggcacggcgg gccgtaggaa tagggagcgg
      541 ctgatcgatc aggagggagc atcgtgcgca gcgattcatg gccggcgctg ccgctggcgg
      601 agtggcagga gacgctcgat accctgcaca tgtggacgca gatcgtcggc aaggtgaagc
      661 tggccctgac gccgttcctg aacgagtggt ggaacgtcgc gctccatgtc aatgcgcggg
      721 ggctgacgac gggggcgatc cccgccggcg ccgggatctt cgacgtcgag ttcgacttcg
      781 tcgatcacat cctggtgatc cgaacgagcg ccggagaacg gcgggagttg gcgctggagc
      841 cgcggtcggt cgcgaacttc tatgcggcgt tcctggcgac gctggccgcg ctgggtatcg
      901 aggtcacgat cgacccgatc ccggccgagc tgccgaaccc gaccccgttc gacgtggacg
      961 agatccatgc cgcgtacgat gcggcggcgg tcgcgcgctt ctggcaggcg ctgatcgaca
     1021 tacagcgggt gctgatgcgg ttccggagcg agtttttcgg gaagagcagc ccggtcctct
     1081 tctactgggg ctcgttcgat ctcgcccatg cgcggttctc gggccggccg attgatccgc
     1141 cggtggggat gccgcgcttt ttccagatcg cggaggacca ggagaacttc gcctgcggct
     1201 ggtggccggg gaatgtcagc gcggcgggaa ttacctttgg cgagccggcc ttctacgcct
     1261 atttcgtccc gtcgccggag gggctgcgca cggcgccgat ccagccggcc ggggcgggct
     1321 gggatacgac gatgggagag ttcatctacc ggtatggcga tgcccggcag gcggccgatc
     1381 cggcggaggc ggtgctggcc tttttccgga gcgtctatga ggccggggcg gacctcgcca
     1441 attgggatcg cgcggttctg gagcgggata tcaggaagga attggtctca tgagccagca
     1501 atgcagtcat cttgatcaga tccgggatgt ggagccgagc gcgcgggggt gcgaggagtg
     1561 cctggcgatg ggggacacgt gggtgcacct gcgggaatgc ctgatctgcg gccatgtcgg
     1621 ctgctgcgat tcgtcgaaga acaagcacgc gacgaagcac ttccacgcga ccggtcatcc
     1681 gatcgtgcag tcgttcgagc cgggtgaaga ctggcgctgg tgctacgtcg acaacatgtt
     1741 cgtgtaaacc ccgagccccc aaggcgcacc atgcatccgc cggcccggtc gatggagccg
     1801 ggccggcgcc ggggttggtt cccgccaacg atcgcgtggg gacggggtcc gggcggccca
     1861 cggggggacc gcgctacgtt atggggcgcg ccattcagcc ggcttcagcc gggttcagat
     1921 gtcagcccgg gtgtttacgc ccgggcgatc ccggccacgc acgtccgcat gcgaacgggc
     1981 ccgtcaggag cggcgggttt gcccccgcaa acccctacaa ttccgcgcct tcgcgcctcc
     2041 gcgcctccgc gcctcagatg agcccctgct ccgtcaggaa gtccttggcc accgtggccg
     2101 gctccgcttt ctcgtcgccg acgacgcggg cgttcatcgc ggtcatcttc tcggtagtga
     2161 ggacaggagc cacggcgttc agggccggag cgagcccggc gttggcgtcg agcgcctcct
     2221 gtcgcacgac cggggcgaca tggtagggcg gccaaagccc cttgtcgtcc tcgagcacga
     2281 cgaggtcgtt gatcgcgatc tcggcgtcgg tgctgaaggc gagaacgacg ttcgcatcgc
     2341 cgtcgatcaa ggccttgtac ttgatgccgg gggcgacgcc gttgaccgac gccttgaagt
     2401 cgccgtaggt cgccttgagc cccttgaggc cgtcatcccg ctcctcgaaa tcggacggcg
     2461 cggagatggt gaggtcgacc gacgaggaga gcgcggcgag ctgcgagatg gtcgtgacgt
     2521 tgttctcctc ggagaacttg cgggtcacgg ccagggcctg ggagtcgttc atcggggact
     2581 ggtcgagcca gacgagattg aactgctcct tgtactgggc cttgacgtag tcgtagaccg
     2641 cctcgaccat gccgcccgct gccggggtcg cgccggccat tgggctggcc gcggcttccg
     2701 gcgtggcagc ggggacatcg gccagcgtct tgccgagcac gaccagcagg ccggtgccgg
     2761 tgtactcggg gtagacgtcg aggtcgccgt tcttgagggc ttcctgggcg acggcggtgc
     2821 cgccgaggtt gagcttccgc tcgactgtca ggccggcgtg ctccagcagg agggcgtaca
     2881 tctcgccgat gatgatcgac tccgggaaat ccttcgagcc gactttgatt gtgctgccct
     2941 gggcgaacgc ggccggtccg acggccaatc ccgtcccgag gaccggcgcg gcggcgaacc
     3001 ccttgatgat cgtgcgacgt gtaaccatgc gaccctttcc tttggtcttg gcggccgcgc
     3061 cacccggttg tggctacggc gcggcgggcg gcgtcaccag acgctcgagc cccgccaggg
     3121 agacttcgac gacgagcgca agcagggcga tcgggaccgc cccgacgaag agcaacgggt
     3181 agtcgagcag ggtcagcccg ctcgtgatga accgcccgag cgagccggcc ccgacgaagg
     3241 cggccagcgt tgcgctcgcc accacctcga cggcggcggt gcgaactccg gcgatgatga
     3301 caggaagggc gagggggagc tgcactttgc gcaacacctg aagctcgctc atgccgagac
     3361 cgcgcgcgtt ctcgagcacg gccggggaga cggcgcggag gccggcgtcg gtattgatca
     3421 gcaggggcgg cccggcgagg aggatcagcg cgatcagggc cagcacgaag gcgcgcggcc
     3481 agaaggggag caggtcgccg atcggtttcc ggtaggggta gagcaggaag aggaccgaga
     3541 tgctcggcac gacccgggca gcagccacca ggccgatcag actcgggccg atccgccggc
     3601 tgcgggcggc gaggatgccg agcgggacaa agatcgcgat cgcggcgaga agggcgtaga
     3661 ggctgagctg gatatgcgtg ctgagggccg cccggaagcg gtccgggttg tcctgaatgt
     3721 actggaggct gtcccggagc agatgcatgc ggttctccgc tcgtgccgca ggtccggatc
     3781 agacgataac acgggtggtt agtggttagt gaatagtggt tggaacgaga ggaacgcccg
     3841 ggggcgggag tgggtcaggc gaggccgttg cggatggcga aggcggccgc ggcggtgcgg
     3901 gagtcgaggg cgagcttggt caggatggag cggacgtgga ccttggcggt tggcacgctg
     3961 atgaagaggg cggcggcgat ctccccgttg gatttgccct gcgcgacgag gcggaggacc
     4021 tccagttcgc gcgggctgag cccgaatgcc ggcgttggcg cggtgggcgc tgattcgcgc
     4081 tcggtaaatg gcgtcaggaa tgccagggac tcggcgaaga cttgatcgcg gggcattgcg
     4141 cgaccggcgg cccaggccgc tgcgaaggcg ttcggactga gcgcggcgcg gatggatgcg
     4201 acgctttgct cgtacgtcgt gcggaacgaa ttgtcctggt acggggttcg cccaagccgt
     4261 tcggcaaccg cttcgtcgga gccgtacagg cgcgccgcca gctccggatt cccggcttcg
     4321 gccacgaaca gggccgggcc atcgagcccg aaatccaggc cgcgctccag gtacagggtc
     4381 acctgttccc ggcagagctg ggcggcgcgg tggacttcgc cgacgcgttg caggaccagg
     4441 ccgaggtttc cggtcgattg cgccaggctg agcacatcgt ccgcttgctg gttcagggcg
     4501 tgggctcgct cgaggtagtc tcgtgctcgc gcgaagtctt cgcgcgggat cgcgaggagg
     4561 ccgagattgt tgagcgcggc ccgggcagca aagtccacgc cgcccgcttg cccgaccgcg
     4621 acggtctcct cgaggtagct gattgccgcc gtatcgtcgc cacattcgct atcgatgccg
     4681 gcgcagagca ggagcgccat gcagagcgcg aggggatcgc cgtcgtctcg cgccgcgggc
     4741 agcgtctgct cggcgtattg cctggcccgg tccaggtcgc cgagcatgag cgacagccat
     4801 cccgtccaat cgaatgcgag cgtccggagc ggcgatggga tatcaggcgc cccggccagg
     4861 atgcgttcca gccgatcccg gccttcgcgg tagcgaccac tgtggtgcca aaaccatccg
     4921 agtgcggcga cgatgcgcag ctgggagacg gcgtcgcccg tttcctcgaa gcggccgagc
     4981 gcggcgcgca ggttgtcgat ctcggcggag acgacagcga accagtgccc cgtgctgctc
     5041 gtccagaccc acgtggagcg ctcggccagg gccaggaaga aggcggcatg gacgtcgcgc
     5101 gtggcggcgt ccaccccttc ggccgcgagc tgctcttgcc cgtactcgcg gatcgtctcg
     5161 agcatttgga agcgggcggc gccctcccgg ccgtcgacct ggcggagcaa gctcgactcg
     5221 acgaggaagg tcaatcccgc cagcgtgtcg atctcccggt catcggaggt caccgcctc
//
LOCUS       DASSGX010000086         3410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_124076, whole genome shotgun sequence.
ACCESSION   DASSGX010000086 DASSGX010000000
VERSION     DASSGX010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124076"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..916
                     /locus_tag="VFI12_01510"
     CDS             <1..916
                     /locus_tag="VFI12_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET7055104.1"
                     /translation="VTELSYPIASSIIREIVLAGGKRLRPLLVLLAGRAYDYHDRFDE
                     LVTAAAGVELLHVASLVHDDTIDRAALRRGKPTLNSVMSTGATILVGDFLFAQSAMLA
                     ARTGDVRVVGVFASTLGELCDGQLLELFDSHKLDQQADTYIRRIYGKTASLFAGAAEM
                     GSVLARAPEPHVQELRQFATEIGLAFQIQDDVLDLTRASDDIGKPAGNDLRQGTVTLP
                     TLLFAQRSNLDPDSDRLLTRVVTDEDVADKDISELIARIRASGAIDDAMATAHRYRDL
                     ALARLAVVPDPDARDLLTRWAHQALGAA"
     gene            complement(919..1515)
                     /locus_tag="VFI12_01515"
     CDS             complement(919..1515)
                     /locus_tag="VFI12_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055105.1"
                     /translation="MIDAATFIEQAPVQDQDLLLRLNLYFDVLDQRLRHGQGWFIFNA
                     GGSRLNRIAGFIQAKLASDHAGVSSYLMPWRDFALSAYVNEVGLAEIGPGKDGRFPSE
                     QQKHEFELARHVTEDTCTRLMYSDMLVLIGMRPSTWHEASFLDRMIDERYRQRLATIL
                     LTSDMPHTLESEFDEVDPSGTLWSRLFGRMYETSLVAL"
     gene            complement(1646..2566)
                     /locus_tag="VFI12_01520"
     CDS             complement(1646..2566)
                     /locus_tag="VFI12_01520"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HET7055106.1"
                     /translation="MAEPTPLILDVDTGVDDALAIGLAVSRTDTDLIAVSTLAGNIDV
                     VNATENTRRVLGLIGASDVPVYQGASRPLARLHRNAAHYHGRNGLGDAVLPEVGFPLG
                     EYRGPAAIIRLARQRPGEIVLVATGPLTNVAIALNVFPELPSLLKRFVVMGGSYRNPG
                     NITPHAEFNIWADPEAAQQVFATDFPDAIAVGLDVSLQTVLLKRAWEEANGPSHALAQ
                     LLSAVCRRSFVDRAQADFPLHDPLATAVALDPTLVATERGVIDVVTSGVRDGKTVFTA
                     NASGSWQVALAVDSGRFVSEFLASYQLGAQ"
     gene            complement(2621..>3410)
                     /locus_tag="VFI12_01525"
     CDS             complement(2621..>3410)
                     /locus_tag="VFI12_01525"
                     /inference="COORDINATES: protein motif:HMM:NF017013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MOFRL family protein"
                     /protein_id="HET7055107.1"
                     /translation="AVRSQLSRVKGGGLRAAILTNQIVSLLLSDVLGNDPTVIASGPT
                     VPLRTTRQDAREVVAELGLADEMPPSVKRVLADNATPAPAVCPDDVVVVVADNKTAVS
                     AAAANLRRRRMRVQIEEIPRTGEARVVAEEWVMRLRDVPLDTDAIVGGGELTVTVKGD
                     GVGGRNTEFALAAAIELERLRIEDWAVASLATDGEDGMTDVAGAIVDCSAPHELRTRH
                     IDPITSLHANDSFPGLDQIGATVKTGPTGTNVNDLYFAIRKPAG"
BASE COUNT          641 a         1113 c         1042 g          614 t
ORIGIN      
        1 agtgaccgag ctgtcatacc cgattgcctc atcgatcatc cgtgagatcg tcctcgccgg
       61 tggcaagcgc ctgcggccgc tcctcgtcct cctggcaggg cgggcgtacg actatcacga
      121 ccggttcgat gagctcgtaa cggccgccgc gggtgtcgag ctactgcacg tcgcctcgtt
      181 ggtacacgac gacacgatcg accgcgcagc cctgcgccgt ggcaaaccga cactcaattc
      241 ggtcatgtcc accggcgcca cgatcctggt cggcgacttc ctcttcgcgc agtccgccat
      301 gctggccgcg cggaccgggg atgttcgggt ggtcggcgtg ttcgcgtcca ctctgggcga
      361 gctctgcgac ggtcaattgc tggagctatt cgattcccac aagctggacc aacaggccga
      421 tacctacatc cgccgcatct atggcaagac ggcgtcgctc tttgccgggg ccgccgaaat
      481 gggctcggtc ctggcgcgtg ctccggaacc gcacgtgcag gagctccgcc agttcgcgac
      541 cgagatcggg ctggccttcc agatccagga cgacgtcctc gacctgacgc gggcgtccga
      601 tgacatcggc aaaccagccg gaaacgacct gcgccaaggc actgtcacgc tgccgacgtt
      661 gcttttcgcc cagcggagta atctcgaccc cgattctgat cggctgttga cgcgagtcgt
      721 gaccgacgaa gacgttgcgg acaaggacat ctcagagctg atcgcgcgaa tccgggcatc
      781 gggcgcgatt gacgacgcga tggcgacggc gcaccggtac cgcgatctgg ccctggctcg
      841 actcgccgtc gtgccagatc ccgacgctcg ggatctgctg acccgctggg cgcaccaggc
      901 gctcggcgcc gcctgaccct aaagcgcgac caagctcgtc tcgtacatcc gaccgaacaa
      961 ccgggaccag agcgtgccgc tcggatcaac ctcgtcgaac tccgattcga gcgtgtgcgg
     1021 catatccgac gtcagcaaga ttgtcgccaa ccgctggcga tagcgctcat cgatcatgcg
     1081 atcgaggaat gacgcctcgt gccaggtact tggccgcatg ccgatcagca ccagcatgtc
     1141 gctgtacatc agccgggtgc atgtatcctc ggtcacgtgg cgggcaagct cgaattcatg
     1201 cttttgctgc tccgagggga accgtccgtc tttgcccgga ccgatctccg caaggccgac
     1261 ttcgttgacg tacgcactga gcgcgaaatc tcgccagggc atgaggtatg agctgacgcc
     1321 ggcgtgatcg gacgcaagct ttgcctggat gaacccggcg atccgattca gccggctacc
     1381 cccggcattg aagatgaacc aaccctgtcc gtggcgaagg cgctggtcca gcacgtcgaa
     1441 gtagaggttc aggcgcaaca gcagatcctg atcttgcaca ggcgcctgct caataaacgt
     1501 agccgcatct atcatcggtg cgacctcctg ctgctcgtaa acacggcatc cggcgaagaa
     1561 aatgccagat ggttctgatt actccgccgg cgattacatc ggctatcacc cgaaaggtgc
     1621 gtatgaggtc cggctggtac ttacatcatt gcgcaccaag ctggtaagac gcaagaaatt
     1681 cggacacaaa ccgaccggaa tcgactgcca gcgccacctg ccatgagccc gaggcattcg
     1741 cggtaaagac agtcttgcca tctcgcacgc cactcgtcac gacatcgatc acgccccgct
     1801 cggtcgcaac cagggtcgga tcgagcgcga ccgccgtggc gagcggatcg tgcaacggaa
     1861 agtcggcctg cgcgcggtca acaaacgacc ggcgacagac tgccgagaga agttgtgcca
     1921 aagcatgcga cgggccattt gcctcctccc acgcccgctt cagcagcacc gtctggagcg
     1981 aaacgtccag cccgaccgcg atcgcatccg ggaaatcggt cgcgaagact tgttgcgccg
     2041 cttccgggtc tgcccagatg ttgaactcag catggggcgt gatattgccc gggttgcggt
     2101 acgacccgcc catgaccacg aatcgcttga gcagcgacgg cagttccggg aagacgttca
     2161 gggcaatggc aacgttcgtc aacggtccag tggcgaccag cacgatctcg cccggccgct
     2221 gacgcgccag ccggatgatt gcagccggac cacgatattc gccgagcggg aatccgacct
     2281 ccggcagcac cgcgtcaccg agcccgttac gcccgtggta gtgggcggca ttgcgatgca
     2341 gcctggcgag cggccgggac gcaccctggt agacaggaac gtcggaagca ccgatcaagc
     2401 caaggacgcg ccgggtgttc tcggtcgcat tgacgacatc gatgttgccg gcgagcgtgg
     2461 agacggcgat caggtcggta tccgtccggc tgacggctag tccaatggca agggcgtcgt
     2521 ccacccctgt atcgacatca aggataagcg gagttggttc ggccacgata tctctttctg
     2581 tccggggttc cgaattccgg gttcgcgcga gtatatcgat tcagccggca ggttttcgga
     2641 tcgcaaaata gaggtcattg acgttcgtgc ccgttggccc ggttttgacc gttgcgccaa
     2701 tctggtccag tcctgggaat gagtcgttcg cgtgcagcga cgtaatcggg tcgatgtgac
     2761 gggtgcgcaa ttcatgcggc gccgagcaat cgacgatcgc gccagcgacg tcggtcatgc
     2821 cgtcctcgcc atcagtcgcg aggctggcga ccgcccaatc ctcgatccga agccgctcca
     2881 actcgattgc ggccgcgagc gcgaactcgg tattgcggcc gcccactcca tcgcctttga
     2941 cggtcacggt cagctcgccg ccaccgacga tggcatccgt gtcgagcggc acgtctcgca
     3001 gtcgcatgac ccactcttcg gccacgactc gcgcctcgcc ggtgcgcgga atctcctcaa
     3061 tctgaacgcg catcctccgt cgtcgcaaat tggctgcagc ggccgatacc gctgtcttgt
     3121 tgtccgccac gaccacgacg acatcatcgg ggcagactgc tggagcaggg gttgcattgt
     3181 cggccaacac cctcttgaca ctggggggca tttcgtcggc aaggcccaat tcggcgacga
     3241 cctctcgcgc atcctgccgc gtcgtccgca atgggacggt tggaccgctg gcgatcacgg
     3301 tcgggtcgtt gccgagcaca tccgagagga gaagcgacac gatctgattc gtcagaatgg
     3361 cggcgcgaag gccaccgccc ttgacccggc tcagctgtga acggaccgcg
//
LOCUS       DASSGX010000087         2493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1211823, whole genome shotgun sequence.
ACCESSION   DASSGX010000087 DASSGX010000000
VERSION     DASSGX010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1211823"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VFI12_01530"
     CDS             <1..515
                     /locus_tag="VFI12_01530"
                     /inference="COORDINATES: protein motif:HMM:NF012626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055108.1"
                     /translation="MKRYSFETKALVGFEKSGHFFFNPPLGRGYDDGIVAALAVCDML
                     DRAPDKSLADLRRALPKTWQSPTMSPHCDDEKKYDVVERVVAHYQKLKADDTPVAGQR
                     ITELITVNGVRVVLEDGTWGLVRASSNKPELVVVVESPTSEENMRAIFADIDSALSKM
                     PEVGEYNQKI"
     gene            638..>2493
                     /locus_tag="VFI12_01535"
     CDS             638..>2493
                     /locus_tag="VFI12_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055109.1"
                     /translation="MHGLPTASAARGRRTIPAGNYLRRTLLATAILLAGGAAASGQPA
                     DTAEALQPGEAFVTRFSGTLDEDKGAARIDPDGVVGSIIDLRAPQQPPEGQHWIDEPQ
                     RAEIRASEVGQVFGVAVDNAGPPNIYVTATSAFGLHLAPGGDWMDGMWGEGGGPGTVY
                     RLDARDGYRPHPFAEIGYEGRANTGAALGNIAFDPHHDQLFVSDLETGMIHRISASDG
                     TDLGSFDHGTDGRPDFLDVPSGETRSLPPIAFDPISSARVDDCPAGPFAQHPECWNFA
                     ASGRRVWGVGVWRHPDTDEVRLYYAVWSGPAFGNTEWDSLSEDEKRNSVWSVRIDADG
                     DFDPSDVRREFLLPDFFVSEEDIAGSGYSHPASDITFSACGERPVMLVAERGGIRNLG
                     LGEEYAFSRPHEARALRYELDRAGAWRPVGRYDVGFYDRREEGQPYLRANCSGGVAFG
                     LGYDEETWVADASRTDEFVWMSGHALCSPKGPCLLPGFEVDVAGEGEEAIQPAAQEEF
                     ADAVPDDSEVHGVQGLAETAFEEIAPAAAFAEHPGDTDAYPATGPGQSFLIDTDTNVD
                     NAGGLVEEELFRNDATRIGDIAIDQPCPAPPRAIRPAVLLPVPTGEPPYLVD"
BASE COUNT          446 a          817 c          810 g          420 t
ORIGIN      
        1 acatgaagcg ctacagcttc gagacgaagg cgctcgtcgg cttcgagaaa tcgggccact
       61 tcttcttcaa cccgcccctc ggccgcggct acgacgacgg catcgtcgca gcgctcgccg
      121 tctgcgacat gctggaccgc gcccccgaca aatcgctcgc cgatctgcgc agggccctgc
      181 ccaagacatg gcagtcgcct accatgtcgc cgcactgcga cgacgagaag aagtacgacg
      241 tagtcgagcg cgtggtggcg cactaccaga aactgaaggc cgatgataca cccgtcgcag
      301 gacagaggat caccgaatta atcaccgtca atggtgtccg cgtcgtgctg gaggacggta
      361 cctggggtct ggttcgcgcc tcgtcgaata agcccgagct ggtggtcgtc gttgaaagtc
      421 cgacgtcgga agaaaacatg cgggcgatct tcgcggatat cgacagtgca ctgtcgaaaa
      481 tgccggaggt tggagagtac aatcaaaaga tttgagcaaa tcatcctccg gagaaatcaa
      541 gtttgcttgg cggatccaag atttgaacga ttgaacgctc ccgtcagcag taggaacgtg
      601 ctaggctgcc caaaacccag aacacggggg gacgcccatg cacggactgc cgaccgcatc
      661 agctgcgcgg ggccggcgca ccatccccgc gggcaactat cttcgccgga cgctgctcgc
      721 caccgcaatc ctgctcgccg gaggagcggc ggcgtcaggc caaccggctg ataccgcgga
      781 ggcgctgcag cctggcgagg cgttcgtgac gcgcttctcc ggcaccctcg atgaggacaa
      841 aggcgcggcg aggatcgacc cggacggcgt tgtcggcagc atcatcgacc tccgggcgcc
      901 gcagcagccg cccgagggcc agcactggat cgacgagcct caacgcgcgg aaattcgggc
      961 cagtgaggtc ggacaggtct tcggcgtcgc tgtcgacaac gcgggcccgc ccaacatcta
     1021 cgtcacggcg acctccgcat tcggattgca cttggcaccc ggcggcgact ggatggacgg
     1081 catgtggggc gaaggcggcg gccccggcac cgtctaccgg ctcgatgcgc gcgacggata
     1141 caggcctcat ccgttcgccg agatagggta cgaagggcgc gccaataccg gcgccgcgct
     1201 cggcaacata gccttcgatc ctcaccacga tcagctcttc gtctcggatc tcgagaccgg
     1261 catgatccac cgcatcagtg cctccgatgg caccgatctt ggctctttcg atcacggaac
     1321 cgatggtcgg cccgacttcc tcgacgtgcc gagcggcgag accaggagct tgccgcccat
     1381 cgcattcgac ccgatcagct ctgcccgcgt cgacgactgc cctgccggcc cgttcgcgca
     1441 gcatcccgaa tgctggaact tcgcagcctc aggccgtcgc gtctgggggg tcggtgtctg
     1501 gcggcatccc gatacggatg aggtacgcct ctattatgcc gtatggagtg ggccggcgtt
     1561 cggcaatacc gaatgggaca gcctttctga ggacgagaag cgtaactccg tctggtccgt
     1621 gcgtatcgat gccgacggag acttcgatcc gtcggacgtg aggcgcgagt ttctgctgcc
     1681 cgacttcttc gtgtcggagg aggacatcgc cggctcaggc tacagtcatc cggccagcga
     1741 tatcacgttc tcggcctgcg gcgaacgacc ggtcatgctg gtggccgagc gtggcggcat
     1801 tcgcaatctc ggcctcggag aggagtatgc cttctcgcgg ccgcacgagg cgcgcgccct
     1861 ccgctacgag ctcgaccgcg caggggcctg gcggcccgtt ggacggtacg atgtcggctt
     1921 ctacgatcgc cgggaggaag ggcagcccta ccttcgcgcc aactgctccg gcggcgttgc
     1981 attcggcttg ggctacgacg aggagacctg ggtcgcagat gcgtcgcgaa cggatgagtt
     2041 cgtatggatg tccggccacg cgctgtgctc gccgaagggg ccctgcctgc tcccgggctt
     2101 cgaggtcgac gtcgctgggg aaggtgagga ggccatccag cctgccgccc aggaagagtt
     2161 cgccgacgcc gttccggacg attccgaggt ccatggcgtt cagggtctgg ccgagaccgc
     2221 cttcgaggag atcgctcccg cggcggcctt cgccgagcac cccggcgata cggacgccta
     2281 tcccgcgacc gggcctggcc agtcgttcct catcgatacg gacaccaacg tcgacaatgc
     2341 cggagggttg gtcgaggagg agctgttccg caacgacgcg acgcggatcg gcgacatcgc
     2401 gattgaccag ccctgccctg cgccgccgcg agccattcgt cctgccgtgc tgctgccggt
     2461 gcccaccggg gagccgcctt atctggtgga cca
//
LOCUS       DASSGX010000088         3222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1103857, whole genome shotgun sequence.
ACCESSION   DASSGX010000088 DASSGX010000000
VERSION     DASSGX010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1103857"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="VFI12_01540"
     CDS             <1..1077
                     /locus_tag="VFI12_01540"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HET7055110.1"
                     /translation="PHAAWARLVARHARALTPAEMAYGDPAGLVPLRVAIAAHLRAAR
                     GVRCEAEHVLVVSGSQAALRLAAAVLLAPGDRVAVEEPGYPGARAALLAGGARLVPAP
                     VDEEGITLAPLRRRGGRVRAVYVTPAHQYPLGTSMTPARRLALLDWASRQAAWVLEDD
                     YDSEYRYVSRPLGALQGMDRHERVIYIGTFSKVLFPALRVGYLVVPPSLWQRFVEARE
                     AFDLFPPPPYQLALAEFLREGDFARHLRRMRGVYLDRRQALLTGLARHCGDRLAVHNA
                     DAGLHVAVLLPVGTDDQELVRRMTERGLTATPLSTCYVGTRRRSGLLLGFGGSTERRL
                     LEATRVLGEVVREVLPAGGLDARD"
     gene            1334..1675
                     /locus_tag="VFI12_01545"
     CDS             1334..1675
                     /locus_tag="VFI12_01545"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET7055111.1"
                     /translation="MVQSAAADEVFHALSNPTRRKVLERLSVGPATVSELAAPFDMQL
                     PSFVQHLSVLEQSRLVKSKKRGRVRTYEIAPERFKVVEDWLTARRRLWEARLDRFDQY
                     VKQLKEKESDS"
     gene            1672..2178
                     /locus_tag="VFI12_01550"
     CDS             1672..2178
                     /locus_tag="VFI12_01550"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET7055112.1"
                     /translation="MSKQFTFNPKLDFAIERFIDAPTRLVWEALTTPEHLKEWYMPKP
                     WGRVSRTEMDVRPGGIFSIDIATADGQEAPNLGCFLDVIPLERLVWTSMLFPGYRPAV
                     FDDIPITAIVTMEPVGTGTRYVFTALHRDEADLEQNKASGFYQGTEMAVDQFVEHVIA
                     MKPNCKRG"
     gene            complement(2220..2672)
                     /locus_tag="VFI12_01555"
     CDS             complement(2220..2672)
                     /locus_tag="VFI12_01555"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7055113.1"
                     /translation="MSVKRMDNVSIVVEDIDAAIEFFTELGLELEGRAPIEGDWADGV
                     TGLRDMRVEIAMMRTPDGHSRLEISRFLAPPVVADHRTAPVNALGYLRVMFTVEDIDD
                     TLARLRKRGAELVGEVAQYQATYRLCYIRGPEGILIGLAQELEPQTTG"
     gene            complement(2839..>3222)
                     /locus_tag="VFI12_01560"
     CDS             complement(2839..>3222)
                     /locus_tag="VFI12_01560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HET7055114.1"
                     /translation="RTKSKSRTTTKKRARNSASETPLVRRRLPTEVPPTHPGEMLLEE
                     FLKPLGISQSAFAIRLGVSFPRLNEVIRGKRAVTPDTALRLAQVTGMSADFWLGLQSD
                     WDLWHALRSAEARQIAKLSPLPRAG"
BASE COUNT          525 a         1057 c         1080 g          560 t
ORIGIN      
        1 ccccatgccg cctgggcgag gctggtggcg cggcacgcgc gtgcgctgac gccggcggag
       61 atggcgtacg gcgatccggc ggggctggtt ccactccggg tcgcgatcgc ggcccatctc
      121 cgtgcggccc gaggcgtccg ctgcgaggcc gagcacgtcc tggtcgtctc cggttcccag
      181 gcggcgctcc ggctggccgc ggccgtgctc ctggcccctg gcgaccgcgt cgcggtggag
      241 gagccgggct atcccggcgc ccgggccgcc ctgctggcgg gcggtgccag gctggtcccg
      301 gcgcccgtgg acgaggaggg catcaccctg gcgccgctcc ggcggcgcgg cggaagggtg
      361 cgcgcggtct acgtcacgcc ggcgcatcag tatccgctcg ggacctcgat gacacccgcc
      421 cggcgcctcg ccctgctcga ttgggcgtct cgccaggcgg cgtgggtgct ggaggacgac
      481 tacgacagcg agtatcgcta cgtcagtcgc ccactcggcg cgctccaggg gatggatcgg
      541 cacgagcggg tgatctacat cggcaccttc agcaaggtcc tctttcccgc cttgcgggtg
      601 ggctacctgg tcgtcccgcc ctcgctctgg cagcggttcg tcgaggcccg ggaggcgttc
      661 gatctcttcc cgccgccgcc gtaccagctg gcgctcgccg agtttctccg cgagggcgac
      721 ttcgcccggc acctgcggcg gatgcggggg gtgtacctcg accggcgcca ggccctgctc
      781 accggcctcg cccggcactg cggtgaccgg ctcgccgtgc acaacgccga cgccgggctc
      841 cacgtggcgg tgctgctgcc cgtcggaacc gacgaccagg agctcgtccg ccgcatgacc
      901 gagcgcgggt tgacggcgac gccgctgtcg acctgctacg tcgggacgag gcgtcggtcg
      961 ggcttgctgc tgggtttcgg tggctcgacg gagcggcggt tgctcgaagc gacccgggtg
     1021 ctgggtgagg tcgtgcggga ggtgctgccg gccgggggac ttgatgcgcg cgattgaggg
     1081 cgcgtggcgg aaccagccgg cggcgactgc ctgacgacag gcgcagcagc tgcaggcgat
     1141 gcccacaccc gcaccagatg ttccttcctt atagctcgag gaacgtgccg accggccggg
     1201 ccttgcgccc caccggcccc tgcatgcgcg gcccttccag gatcgcaaat acttaggtat
     1261 ttacaaaagt attccacggc tgtagactcc ctcggtcaga tcggataacc cgggcattcg
     1321 gatggtgtcg gaaatggttc aatccgcagc cgctgacgaa gtttttcacg ccttgtccaa
     1381 cccgacgcgg cggaaagtcc tggagcgact gtctgtcgga ccagccaccg tcagcgaact
     1441 ggcggcaccg ttcgacatgc aactcccgtc gttcgtgcag cacctctcgg tgctcgaaca
     1501 gagccggctg gtgaagtcga aaaagcgcgg gcgagtacgg acgtatgaga tcgcacccga
     1561 acggttcaag gtcgttgaag actggctgac cgcgcgtcgc cggttgtggg aagctcgact
     1621 ggaccggttc gaccaatacg tcaaacaact caaggagaag gaatcagact catgagcaag
     1681 caattcacct tcaatccgaa acttgatttc gctatcgaac ggttcatcga cgctcccacg
     1741 cggctcgtct gggaagcgtt gacgacaccg gagcatctca aggagtggta catgcccaag
     1801 ccctgggggc gtgtgtcgcg aaccgaaatg gacgtgcgac cgggcggcat cttcagcatc
     1861 gacatcgcaa cggcagacgg tcaggaggct cccaacctcg gctgtttcct ggacgtcatt
     1921 ccgctggagc gactggtctg gacctccatg ttgttccccg gctatcgccc ggcggtcttt
     1981 gacgacattc cgatcaccgc catcgttacg atggaacccg tgggaaccgg cactcgctac
     2041 gtcttcacgg cgctgcaccg ggacgaggcg gacctcgagc aaaacaaggc gtcgggcttt
     2101 taccagggca ccgagatggc cgtcgatcag ttcgtggagc acgtgatcgc gatgaagcct
     2161 aactgcaagc gcgggtgagg cagcacggcc tagatcaggt cagcgccggt caagggtgct
     2221 catccggtag tctgcggctc cagttcctgg gcgaggccga taagaatgcc ttccggcccc
     2281 cggatgtagc agagacgata cgtggcctgg tactgggcca cttctcccac gagctccgcg
     2341 cctcgtttgc ggagccgggc gagcgtatca tcgatgtcct ccaccgtgaa catgacgcgc
     2401 aggtagccga gcgcgttcac gggggcggtc cggtgatcgg cgaccacagg cggcgcgagg
     2461 aatcgtgaga tctcgagccg gctgtggccg tccggggtgc gcatcatggc gatctcaaca
     2521 cgcatatctc gcaatccagt gaccccatct gcccagtccc cttcgatcgg ggcgcgccct
     2581 tcgagctcga ggcccagttc ggtgaagaac tcgatcgcgg catcgatgtc ttctaccacg
     2641 atactgacgt tgtccatccg cttgacgctc actgaggctc catgtgccga tgttacgagc
     2701 aagcttccag tgcttcctca cgcagagcta acccgcgagc ggatggaaca agatgcggcg
     2761 gcgtagccgc cgccctcaat cgcgcgctcg tcgggttcag cgcctcgttc gatggtcgca
     2821 ccgcgatgct gacgctcgtc aaccggcgcg aggcagcggg gatagcttgg cgatctgacg
     2881 ggcctccgca gaccgtagcg catgccagag atcccagtcc gactgcagcc ccagccagaa
     2941 gtcggcactc atgccagtta cctgggccag gcgcagggcg gtatcgggtg tgaccgcccg
     3001 tttgccgcgt atgacctcgt tcaagcgcgg gaacgaaacg ccgagccgaa tggcgaacgc
     3061 agactgcgag ataccgagcg gcttgagaaa ctcttccagg agcatctcgc ccggatgtgt
     3121 cggaggcacc tccgtcggga gccgtcgacg aacgagcggc gtctcgctgg cgctgttgcg
     3181 agcccgcttc ttagtggtag tccgtgactt cgactttgtc cg
//
LOCUS       DASSGX010000089         4415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1430783, whole genome shotgun sequence.
ACCESSION   DASSGX010000089 DASSGX010000000
VERSION     DASSGX010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4415
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1430783"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VFI12_01565"
     CDS             <1..211
                     /locus_tag="VFI12_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055115.1"
                     /translation="EFAADPARATISSLYVDAVVETPRGAWPFGCAGEYEPDLAYLTA
                     FVRASRDREAQQAFIRERVLEAVPA"
     gene            208..936
                     /locus_tag="VFI12_01570"
     CDS             208..936
                     /locus_tag="VFI12_01570"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HET7055116.1"
                     /translation="MTEPTREEVRDYMVVALARLLRDGETVFHGVASPIPMVATLLAK
                     ATHAPNLVYLNIAGGVDPEPATLPVSTVDPSLLRGARSMVTLTDLFDLAASQRLDTAF
                     LSGVQIDGQGRTNMSVIGPFARPKVRLPGGAGSAAIMPRAKRTLLWRTKHDRRVFVDT
                     LDFVTAAGNVDRVVTPLCVFRRMDGQLVVESRHPWASPEQLIEATGFPICVDAATPIT
                     LEPTAGERDCLARIDSDGVTLTEF"
     gene            complement(955..1290)
                     /locus_tag="VFI12_01575"
     CDS             complement(955..1290)
                     /locus_tag="VFI12_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055117.1"
                     /translation="MRAVAGRADARSHRDLSGNFGKSELYSPGGNLLRAISGDKCLCD
                     EPGKGQILSIKRGISHHVPKVPSLYDLIPDHNPGNQISQYVRVFLAMCVRTTPKPTYQ
                     PDVEMATLC"
     gene            1498..1782
                     /gene="rpsP"
                     /locus_tag="VFI12_01580"
     CDS             1498..1782
                     /gene="rpsP"
                     /locus_tag="VFI12_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HET7055118.1"
                     /translation="MLKIRLRRMGAKKRPSYRIVVAESSSPRDGRFIEIVGTYDPLTD
                     PATIRLNEERAKHWLSVGAQPTDTVRDILRKANLVPDDPKLAARGQQSAA"
     gene            1920..2267
                     /locus_tag="VFI12_01585"
     CDS             1920..2267
                     /locus_tag="VFI12_01585"
                     /inference="COORDINATES: protein motif:HMM:NF024483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HET7055119.1"
                     /translation="MDSENQMDSENQVDSEDQVDSEEQVDSEELVSATPAESDLAELV
                     RYVAAKLVHNQDDISVDSDMRAGTVYVNLRVPEEEMGRVIGREGRTAKAIRTLVSIAG
                     SRHNVHARLDIDG"
     gene            2365..2874
                     /gene="rimM"
                     /locus_tag="VFI12_01590"
     CDS             2365..2874
                     /gene="rimM"
                     /locus_tag="VFI12_01590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HET7055120.1"
                     /translation="MVGRVAGTHGLNGELKVVLTTDDPENLLTVEQLLVGEDRIPHAI
                     ASIRFHKGMALIAFDDIVDVDAAEALRGSVLRISGAHVRPLAENEYYLYQVAGIEART
                     ETGEVIGTVTDVIETGANNVFVVTPVDGGKELLFPSIPDVVIDLQPAEGYMVVRPQIW
                     WDEEQNSTT"
     gene            complement(2931..3710)
                     /locus_tag="VFI12_01595"
     CDS             complement(2931..3710)
                     /locus_tag="VFI12_01595"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HET7055121.1"
                     /translation="MSVVAVLVAMEAELVHLRNLATSSSPRSIAHWEAYDLSLGDHTV
                     VAVKSGIGMLNAAAAAERTMCEINPDIMLNYGCAGAHRRDIMPGDVIIGAQTVNHAAL
                     NILPDGSEVHNDRGNLNSGEQIFPAVIDADAVLLAKATEVAAGISFAPWPADVVWPKS
                     IPHRAPEVFVGPLASSEIWTQNLDRLDVLHARHQSLSEDNESGAFAHVAFIHDVPFLS
                     VKDIANNEYFASSDLAEFSDFPTGEVGKRAASLIAALIKAL"
     gene            complement(3724..4068)
                     /locus_tag="VFI12_01600"
     CDS             complement(3724..4068)
                     /locus_tag="VFI12_01600"
                     /inference="COORDINATES: protein motif:HMM:NF019459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1634 domain-containing protein"
                     /protein_id="HET7055122.1"
                     /translation="MATTDSMYHDVAQVLKTGFRIAAGLIVAGLAIAIARHQPLQTHV
                     DPISKIPDALIHLHSSAFIDLGIIGIMLTPLASVFTILRTFIARGDRTFAMYTLGVLV
                     ILSASITLSLLR"
     gene            complement(4068..>4415)
                     /locus_tag="VFI12_01605"
     CDS             complement(4068..>4415)
                     /locus_tag="VFI12_01605"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET7055123.1"
                     /translation="AGLMSGMLGVGGGVIKVPVMNVLMRIPVKAAAGTSTFMVGITAV
                     ASSSVYYSRGKIDPTIVIPAMIGIYLGSQFGSRLTRRLHAERLMLIFVLILAYLSISL
                     FAKAFGYTLPGQQ"
BASE COUNT          810 a         1365 c         1464 g          776 t
ORIGIN      
        1 ggagtttgcg gccgatccgg cgcgggcgac gatatcgtcg ctctacgtcg acgcggtggt
       61 cgagactccg cggggcgctt ggccgttcgg atgcgcgggc gaatacgagc cggacctcgc
      121 ctacctgacc gcgttcgtgc gagccagtcg cgaccgggag gcacagcagg cgtttattcg
      181 ggagcgcgtg ctggaggcgg tgccggcgtg actgagccga cgcgcgaaga agtccgggat
      241 tacatggtcg tggcgctcgc ccgcctcctg cgggacggtg agacggtctt ccacggagtc
      301 gcatcgccaa tcccgatggt cgcgaccctg ctcgcgaagg cgacgcatgc gccgaacctc
      361 gtttacctga acatcgcggg cggcgtcgat cccgaaccgg ctacgctccc ggtgtcgaca
      421 gtcgatccct ccctgttgcg cggcgcccga tcgatggtca cgctcacgga cctgttcgac
      481 ctcgcggcga gtcagcgact cgacaccgcc tttctgagcg gcgttcagat cgatgggcag
      541 ggcaggacca acatgagtgt gatcggccca ttcgccaggc cgaaagtgcg actgccgggc
      601 ggcgcgggga gcgcggcaat catgccgcgg gccaagcgga ctctgctctg gcggaccaag
      661 cacgaccggc gggtcttcgt cgacacgctc gacttcgtca cggccgccgg caacgtcgat
      721 cgggtagtca cacccctgtg cgtgttccgg cggatggacg gccagttggt cgtcgagtcc
      781 cggcacccgt gggcgagccc ggagcagttg atcgaggcca ccggcttccc gatttgtgtg
      841 gatgctgcta cgccgatcac ccttgaaccg acagccggcg agcgggactg cctcgctcga
      901 atcgattcag acggcgtgac attgacggaa ttctgaggta attcccggta acttttagca
      961 aagtgttgcc atctcgacat ctggctggta ggttggtttg ggtgttgtgc gtacgcacat
     1021 ggctaggaac acccggacgt attggctgat ctggttgccg gggttgtggt cggggatcag
     1081 gtcgtagagg gaagggactt tcgggacatg atggctgatg cctcgtttga tggacaagat
     1141 ctgaccttta ccaggttcgt cgcagaggca cttatcgcca gagatggccc gcaagagatt
     1201 tccgcccggg ctgtagagct cggacttccc gaaattgccc gagaggtcgc ggtggctgcg
     1261 tgcgtcggca cggcctgcga ctgctcgcat gtccagccgc gagacttcat ggacgcggcg
     1321 tactagttga ccactcacgc ctgacgatgc gatactggga cgcgctgccc gcctaaaggg
     1381 gagcgcgttc cgcgtgtcca atttctcctc catgttgggc cgccgctgaa catctgacat
     1441 cgaacggttc gaacatgact ggccggatgc ggccaatttc cgcaagggag agagtgcgtg
     1501 ctaaagattc ggttgcgccg gatgggcgcc aagaagcgcc ccagctatcg gattgtggtc
     1561 gcggagagca gctctccgcg cgacggccgg ttcatcgaga tcgtgggcac ctacgatccg
     1621 ctgaccgacc cggcgacgat ccggctgaac gaggagcgcg cgaagcattg gttgagcgtc
     1681 ggcgcccagc cgacggacac ggtccgcgac atcctgcgca aggcgaacct tgtcccggat
     1741 gatccgaagt tggccgctcg cgggcagcag agcgcggcct agagcggcgc ccggggtcgg
     1801 cggacacgcg ccgggtcctg atatgctgat tcatcggtgg ggcgatccga ttcgctgatc
     1861 ccccgccgat ttgttttacc acccgtttca atgccaggaa cggctagccg gagcagggta
     1921 tggattccga aaaccagatg gactccgaga atcaggtgga ctccgaggac caggtggatt
     1981 ctgaagagca ggtggattct gaagagcttg tcagcgccac ccccgccgag tcggaccttg
     2041 ccgaattggt ccggtacgtt gccgcgaagc tcgttcacaa ccaggacgac atctcggtgg
     2101 attcggacat gcgcgccggc acagtctacg tcaacctccg cgtgccggaa gaagagatgg
     2161 gccgcgtgat cggtcgcgaa ggccgaacgg ccaaggcaat ccgcacgctc gtctcaatcg
     2221 ccggttcccg ccacaacgtc catgcccgac tcgacatcga cgggtaagcc cgaaacacgc
     2281 cgcaagaaac gccgattcac accgcccaat ctggccagga cacgcggacc gaatcctgac
     2341 gccccactgg agcaggtcca gctcatggtc ggccgggttg ccggcacgca tgggctgaac
     2401 ggcgagctga aggttgtcct gacaacggac gatcctgaga atctcctcac cgtggaacag
     2461 ctgctggttg gtgaggaccg gatcccacat gcgatcgcat cgatccgctt ccataagggc
     2521 atggccctga ttgcgttcga tgacatcgtc gacgtcgatg cggccgaggc gctgcggggg
     2581 agcgtgctgc gcatctccgg cgcccacgtg cgccctcttg cggagaacga gtattacctc
     2641 taccaggtcg cggggatcga ggcccgaacc gaaacgggcg aagtgatcgg cacggtcacc
     2701 gacgtgatcg agaccggcgc caacaacgtt ttcgtcgtca ctccggttga cggcggcaag
     2761 gagctgctct tcccgagcat ccccgacgtc gtaatcgacc tccagcccgc cgagggttac
     2821 atggtcgtcc gcccccaaat ctggtgggac gaagagcaga actccacgac ataacgtagc
     2881 gcggggctac ggtgtccgcg gggctggtaa ccccgcgttc cttctcgggt tcagagcgcc
     2941 ttgataaggg ccgcgatcag gctggcggcg cgcttgccga cttcgccggt cgggaaatcg
     3001 ctgaactccg ccagatcgct cgacgcgaaa tactcgttgt tcgcgatgtc cttgactgag
     3061 aggaagggga catcgtggat gaacgcgacg tgggcgaacg cgcccgattc gttgtcctcc
     3121 gacaggctct gatgccgcgc gtgcaggaca tccagccggt ccagattctg cgtccagatc
     3181 tccgacgagg cgagcgggcc gacgaacacc tccggcgccc ggtgcgggat cgatttcggc
     3241 cagaccacat cagccggcca cggcgcaaag gagatgccgg cagcaacctc cgtcgccttc
     3301 gccagcagca ccgcatcggc atcgatgacc gccgggaaga tctgctcgcc cgagttcagg
     3361 ttgccgcggt cgttatggac ctcggagccg tccgggagga tgttcagcgc cgcgtggttg
     3421 accgtttgcg cgccgatgat cacgtcgccc ggcatgatgt cccgccggtg cgcgccggcg
     3481 cagccgtagt tcagcatgat gtccggattg atctcgcaca tcgtgcgctc ggccgcggcg
     3541 gcggcgttga gcatgccgat cccggatttg acagcgacaa cggtgtggtc gccgagcgag
     3601 aggtcgtacg cctcccagtg cgcgattgaa cggggagagg acgaggtcgc gaggttccgg
     3661 aggtggacca gctccgcctc catcgcgacc aggactgcaa cgactgacat gacgaccgaa
     3721 ctcctatctc agtaacgaaa gcgtgatgct ggcggagagg atgacgagca cgcccagggt
     3781 gtacatcgca aacgtgcggt cgccccgggc gatgaacgtg cgcaggatgg tgaacaccga
     3841 ggccagcggc gtgagcatga tcccgatgat cccgaggtcg atgaaggcgc tgctgtgcag
     3901 gtgaatcagc gcgtccggga tcttgctgat cggatcgaca tgcgtttgga gcggttggtg
     3961 ccgggctatc gcaatcgcca gcccggcgac gatcagcccg gctgctattc ggaacccggt
     4021 tttcagcacc tgggcgacgt cgtggtacat cgaatccgtc gttgccatca ctgttgcccc
     4081 gggagcgtgt acccgaacgc cttcgcgaag agcgagatgc tcaggtaggc caggatcagg
     4141 acgaagatca gcatcagccg ctcggcatgg agccgccgcg tcaggcgaga gccgaactgc
     4201 gatcccaggt agatgccgat catggccggg atgacgattg tcgggtcgat cttgcctcgc
     4261 gagtaataga ctgacgacga ggcgacggcc gtgattccga ccatgaaggt gctcgtgccg
     4321 gcagccgcct tgaccgggat ccgcatcagc acgttcatga ccggcacctt gatcacgccg
     4381 ccaccgacgc cgagcatgcc ggacatcagc cccgc
//
LOCUS       DASSGX010000090         3916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_124714, whole genome shotgun sequence.
ACCESSION   DASSGX010000090 DASSGX010000000
VERSION     DASSGX010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3916
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124714"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            236..538
                     /locus_tag="VFI12_01610"
     CDS             236..538
                     /locus_tag="VFI12_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055124.1"
                     /translation="MVTSFEEASVHVPLDSTSPGADRLPSVGTTLEPGQLGTLREMGW
                     SVECRGCYWLCSREHRRIELFRDGRVIFKDPTITARYQLDDEWLKVIRFVWPNVQF"
     gene            664..1098
                     /locus_tag="VFI12_01615"
     CDS             664..1098
                     /locus_tag="VFI12_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055125.1"
                     /translation="MSHKVHAADPTIPFKPIRRFDVFAEVNRLKALREGRPEDEAKGY
                     GIWLAKVVASRRYGQKTDGDSREKAGTKEKPEKSPADEGFKAVGGELQTDETFDSEII
                     DRMGGRFYDETFAPAIKAAVESGKKYEDIRDSIRADWKPRRG"
     gene            1199..3823
                     /locus_tag="VFI12_01620"
     CDS             1199..3823
                     /locus_tag="VFI12_01620"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7055126.1"
                     /translation="MELLERESCIAELQAHVLAASAGQGRVVLLGGEAGIGKSMLVRR
                     FADQTAASTRVLIGGCDALAHPQPLVPLRDVARELGEPVGELIRDHDRRSELFRAVLD
                     ALHAAGGSVFVIEDVHWADEATLDLLRYLGRRIGSTRALVIATYRDDEVGGQHPLRFV
                     LGDLATAAHVRRMSIPPLSASAVRQLANDSAIDPAALHVQTGGNPFFVTEVLGSGMIG
                     VPPTVREAVLNRAARLSDSARAILDVAAVIGSRIEPRLLMAVGGVSADPIDECLAAGL
                     LQAWEGAIGFRHELARDALLSAISPARGVELHGRVLAGLLSGPVPSDDFARLAHHADA
                     AGDGEAVLRFAPVAARRSSELGAHRAAAAQYARALRYAARLPDGERQELLHARLVACH
                     LSGQSTESLELANELLGLARDAGDREVEAEYLSWKASVLVSAGQNAGAEQASRAALER
                     VTGLAPARSHAFVYCTQAQLRMLHRDRDEAILWGTQAAAIAEQFGDIEAQVRALNASG
                     SARLVADVEDALGRADLEESLAVARTARMDSEVAGAYTNLGSSFGEGYRFALAEQYLT
                     DGLTFTGERDLDRWHWYMVAWLALVRMYQGRWSEATELAQEVISSAGTTVISRIMALV
                     ALGRVRARRGDPEVWTLLDEALALAEPTGTLQRLAPVRAARAEAAWLAGDRGRTVAEA
                     CAVFNLALEHRHQWHIGELAYWRWTAGELTSYPPAAEPFGRQIAGDWSVAATLWRELG
                     CPYEAARALGEGEDESAMRASLTGFDQLGARPMAATMTRRLREMGATGIPRGPRASTR
                     ENAARLTARELEVLRLVARRQTNAEIANHLFLSAKTVQHHVTAILGKLGATTRWEAAT
                     LAMQRRIIPSGDDGEAPR"
BASE COUNT          641 a         1209 c         1386 g          680 t
ORIGIN      
        1 gagtcgatca acgtttcggg ctaccccggc gcgggctggg agctcgtcta cgacggcgag
       61 acgaagattt accgccgcac gaagtgacct ccagcaccgt ctgtaaccaa ttacgaacac
      121 tgtgcatcat ccgtagccgg cgctgcttcc ctttcggttc ccgcctccct agagtaggtg
      181 tacgaccacc tgtcaatggg gatgggtgcg cgtgcatttc gggggtgaag tcgtcatggt
      241 tacgtcgttc gaagaagctt ccgtccatgt cccgctagat tcgacgtcgc cgggagccga
      301 ccggttgccg tcggttggga caacgctcga gccgggacag ctcggcacgc ttcgcgagat
      361 ggggtggagc gtcgagtgcc gcgggtgcta ctggctttgc tcgcgcgagc atcgccggat
      421 cgagctgttc cgcgatggcc gcgtgatctt caaggaccct acgattactg cccggtatca
      481 gcttgatgac gaatggctca aggtgattcg cttcgtctgg ccgaatgttc agttctgagt
      541 tcctgacgat aactgactgg tagtgatgat gcggccaggt gcgccgcgtc atcgctacgc
      601 tctgttcagg acccggttga cctggctcca cgggcagcgc cgggtcacga cgaggtgatg
      661 gatatgagcc acaaggtgca tgccgccgat ccaacgatcc cgttcaagcc gatccggcgg
      721 ttcgatgtct tcgccgaggt gaaccggctc aaggcgctcc gggaggggcg gcccgaggac
      781 gaggcgaagg gctacgggat ctggctggcc aaggtcgtag ccagtcgccg gtacgggcag
      841 aagaccgacg gcgacagtcg ggagaaggcc gggacgaagg agaagccgga gaagagcccg
      901 gccgatgagg ggttcaaggc ggtcggcggc gagctgcaaa cggacgagac gttcgattcc
      961 gagatcatcg accggatggg cggccgattc tatgacgaga ccttcgcgcc ggcgatcaag
     1021 gcagccgtcg agtcgggcaa gaagtacgag gacatccgcg actccatccg ggcggactgg
     1081 aagccgcgca ggggctgatc gcgccctcgc cctgtaccat cggcaattcg tgacaacagg
     1141 acctggaatc acccgccacc cgtggcacat ggcgctcacg gggtagtagg gtgacgcaat
     1201 ggaactgctt gaacgggaat cgtgcattgc ggaattgcag gcacacgtac ttgccgcgag
     1261 cgctggacag gggcgagtcg tgctgctcgg tggcgaggcc ggcatcggga agtcgatgct
     1321 cgtgcgtcga ttcgcggacc agaccgctgc gtcgacgcgg gtcctgatcg gcggctgcga
     1381 cgcgctcgcg cacccgcaac cgctcgtccc gttgcgtgat gttgcgcgtg aactggggga
     1441 acccgtcggg gagctgatca gggaccacga ccggcgatcg gagctgttca gggctgtgct
     1501 ggacgcgctg catgcggcgg gtggctcagt attcgtcatc gaagacgtgc attgggctga
     1561 cgaggcgacg ctcgatctcc tccgctacct tggccgccgg attggatcta cccgtgccct
     1621 ggtgatcgcg acgtaccggg acgacgaggt cgggggccag catccgcttc gcttcgtcct
     1681 cggtgatctc gcaaccgccg cccatgtgcg acggatgtcg ataccaccgc tttcggcatc
     1741 ggccgtgcgg cagttggcca atgacagcgc gattgacccg gcagccctgc atgtccagac
     1801 tggcggaaac ccgttcttcg tcacggaagt gctcggatcc gggatgatcg gcgtcccgcc
     1861 gacggtgcgc gaggcggttc tgaatcgcgc agcccggctg tctgattccg cccgggcaat
     1921 cctggacgtc gctgccgtca tcggatcccg gatcgagccg cggctcttga tggcggtcgg
     1981 cggtgtctcg gccgatccaa ttgacgaatg cctggcggcc ggattgctcc aggcttggga
     2041 gggcgccatc ggtttccggc atgagctcgc gcgagatgcg ttgctcagcg ccatttcccc
     2101 cgctcgaggc gttgagctgc atggccgtgt tctggcaggg ttgctctccg ggccagtacc
     2161 gtccgacgat ttcgcacggt tggcgcacca tgcggatgcc gccggtgatg gggaggccgt
     2221 cctgcgcttt gccccggtcg ccgcgcggcg gtcgtccgag cttggcgcgc atcgtgcggc
     2281 agcagcgcaa tacgcccgtg cgctgcggta tgcggcgcga ctgcctgacg gcgagcgaca
     2341 agaactgctg catgcccggc tcgtcgcctg tcacctcagc gggcaatcga cagaatcgct
     2401 cgagctcgcg aacgagctgc tcggattggc tcgcgacgcc ggcgaccggg aagtggaagc
     2461 cgagtatctc tcctggaaag ccagcgtcct cgtcagcgcc ggacagaatg ccggggccga
     2521 gcaggcgagc cgggccgccc tcgagcgtgt gaccgggttg gcgccagccc gatcccacgc
     2581 cttcgtctat tgcacgcaag cccagttgcg catgttgcac cgcgaccggg acgaggcgat
     2641 cctgtgggga acccaggcgg cggcgatcgc agagcagttc ggggacatcg aagcccaggt
     2701 gcgggcgctc aatgcctcgg gcagcgcgcg actcgtggcc gatgtcgagg atgccctggg
     2761 ccgggcagac ctcgaggaga gcctggcggt ggcgcggacc gcgcgcatgg attcagaagt
     2821 cgcgggcgcg tacacgaacc tgggttcgtc attcggcgag gggtatcgct tcgcgcttgc
     2881 cgagcagtac ctgaccgacg gattaacctt caccggcgag cgagacctcg atcggtggca
     2941 ctggtacatg gttgcgtggc tcgctctggt ccgcatgtac cagggacggt ggtctgaggc
     3001 gacggagctg gcgcaggagg tcatctccag tgctggcacg acggtgatca gccgcatcat
     3061 ggccctcgtt gcgcttggtc gggttcgcgc ccggcggggt gaccccgagg tctggacgct
     3121 tctcgacgaa gccttggcgc tggctgagcc gacgggcacg ctgcagcgcc tggcgccggt
     3181 gcgggcagcg cgggccgaag cagcctggtt ggccggggat cgcgggcgaa ccgtggccga
     3241 ggcgtgcgcg gtgttcaatc tcgcgctcga gcaccgtcac cagtggcata tcggagagct
     3301 cgcctattgg cgctggacgg ccggcgagct gacatcgtat ccacctgccg ctgaaccatt
     3361 tgggcggcag atagccggtg attggtccgt agcggcgacg ttgtggcggg aactgggctg
     3421 cccctatgaa gcggcgcgcg cccttggcga gggcgaggat gaatcggcca tgcgggcgag
     3481 tttgacagga ttcgaccaac tcggcgctcg gcccatggcc gcgaccatga ccaggcggct
     3541 gcgcgagatg ggcgccacgg ggatcccgcg cgggccccgg gcctctaccc gcgagaacgc
     3601 ggcgcggctg acggcgcggg agttggaggt gctgcggctc gtcgcccgtc gccagacgaa
     3661 cgcggagatc gcgaaccacc tctttctctc agcgaagact gtgcagcatc acgtgacggc
     3721 gattctcggc aagctcggcg ctaccacgcg gtgggaggcg gcgaccctcg cgatgcagcg
     3781 ccggatcatc ccgtcaggcg acgacggcga agccccaaga taggcatctc attccaccaa
     3841 tatcagggtc ctttcccgat gtcgcgatgc cgtgggggca ataccatgtg tcatgccggc
     3901 cggcgaaccg attcgc
//
LOCUS       DASSGX010000091         4795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1322382, whole genome shotgun sequence.
ACCESSION   DASSGX010000091 DASSGX010000000
VERSION     DASSGX010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4795
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322382"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VFI12_01625"
     CDS             complement(<1..529)
                     /locus_tag="VFI12_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055127.1"
                     /translation="MRKSWGDADMQLAVSELPPIAGHILRPVRAAANTAIFLLKVLPT
                     LPSRPVDWVTATPVRERVCYPTRDGVVEGELTRPGTAGPHPGVLLCLGVVPFDKDHPQ
                     VPRLQEALARAGFASLLYWSPAMRDYRLDPADVENLALAYDWLIDHDGIDPQRSGMIG
                     TCVGGAFVLMAAAQER"
     gene            691..1896
                     /locus_tag="VFI12_01630"
     CDS             691..1896
                     /locus_tag="VFI12_01630"
                     /inference="COORDINATES: protein motif:HMM:NF018479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HET7055128.1"
                     /translation="MQRPWVQRAMVGRDIAEAHRASTPLELLFDLCFVVAVSQAVTGL
                     HHFAADGRFADGVLRYGLAFFAIWWAWVNFSWFASAFDTDDVAYRVVVLVQIAGNLVI
                     AAGIPRFFEHMDLKVMVAGYVIMRLGLVSLWLRVARSDSIAAPTARRYALGVTLVQVG
                     WAAALLVPDQARVPDFIVLILIEMAVPVWAERPGMTHWHPGHIAERYGLFTVIVLGES
                     ILGATVSIQSAFDAGEEVTSLIMIATGGLLIVFAMWWLYFDQPTDGSLRRFWQGEAGP
                     RESPFIFGYGHLFIFASAAAVGGGLEIAVDYATHHTEISASQAAAMIAGPVALYLVCI
                     WLLRFTLHQSPSLKTALYAIAVVLVLATMLLSQPALPIGLITAGLVTALVVVRWRIET
                     RASTPQPAD"
     gene            complement(1984..4611)
                     /gene="ppdK"
                     /locus_tag="VFI12_01635"
     CDS             complement(1984..4611)
                     /gene="ppdK"
                     /locus_tag="VFI12_01635"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HET7055129.1"
                     /translation="MGRWIRLFTEGDASQRDLLGGKGANLAEMTKLGLPIPPGFTVTT
                     EACTEYLRRGGKVPEGLWEEVEYGIRYTEGRLGHRLGDPAKPLFVSVRSGAAASMPGM
                     MDTILNVGLNDATAEGLGRLVDERFALDAYRRLIQMFGKVVLDAPAERFDDALSGARL
                     ALGVKSDHELTPVALRRLIIHFREILDAQGTVLPDDPREQVREAILAVFRSFNTERAK
                     SYRQMHGLSDDMGTAANIQAMVFGNMGADSATGVAFTRNPNTGAPGLFGEFLQNAQGE
                     DIVAGTRTPRPISEMFGDPVFHDAGTQLQALADQLETHFLDMQDIEFTVEQGRLWILQ
                     TRTGKRTAQAAVRIAVDLANAGSIDRTEAVHRVSPAQIELLMHPHIDPSRDLAVIAAG
                     LPASPGAASGAVVLTTAEAKRRGEAGEAILLVRPETAADDFPGMRWVKGILTARGGMT
                     SHAAVVARGMGTPAVVGCSALEVDERSGVIRIGSTVIRAGDDLTIDGSTGHVITGKVA
                     MVPAHLDEFAQTLLSWADDIRRLHVRANADTVADTQRARSLGAEGIGLCRTEHMFFSD
                     GRLHVVRTLILAEDVVERQRALAKLEQFQFGDFAGIFRAMDGLPVTIRTLDPPLHEFL
                     PQRTDEIAQLASRIGMSVAAVEARIEELREANPMLGHRGCRLGMTVPEITEMQARAIF
                     RAALLCAMEGVDVRPEIMIPLVSDVEELRRQRKVIDDVATRIFANAGRSVAYRVGTMI
                     ELPRAALRGADLATEADFFSFGTNDLTQTVYGLSRDDAGTFLPQYLAEGIYDQDPFQV
                     LDREGVGELIRIAAERGRAAKPNLELGVCGEHGGDPDSIQFCHEIGMDYVSCSPYRVP
                     VARIAAAHAALGTMAAVVV"
BASE COUNT          759 a         1590 c         1586 g          860 t
ORIGIN      
        1 ctcgctcctg ggccgcggcc atcagtacga acgcgccgcc gacgcaggtg ccgatcatgc
       61 cgctgcgttg cgggtcaatc ccgtcgtggt caatcaacca gtcgtaggcc agcgccaggt
      121 tctcgacgtc ggcgggatcg agacggtaat cgcgcatcgc cggcgaccag tagagcagcg
      181 aggcaaaccc ggcccgggcc agcgcttcct gcaggcgcgg cacctgcgga tgatccttgt
      241 cgaacggcac gacaccgagg cagagcagca cgcccggatg tgggccggcc gtccccggcc
      301 gagtcaactc gccctcgacc actccgtccc gcgtcgggta gcacactcgc tcccgcaccg
      361 gtgtcgccgt cacccaatcc acgggccggg agggtaacgt cggcagcact ttgagcagga
      421 agattgccgt attggcagcc gcccgtaccg gccgaaggat gtgacctgca atcggcggta
      481 attccgaaac ggcgagctgc atgtccgcat ctccccacga cttcctcatc gggcactccg
      541 ttggagagta cgccggaacc gatcaacgcg acgtgacact acgcatgctc ggcatgtcag
      601 ggctgcaaca acgcagacgc gcttgaagcg gaccttcgct ggctcgtatc gtggccgtgc
      661 cgggcggcag gttaacgggt ggagggggcg atgcagaggc cgtgggttca gcgggcgatg
      721 gtcggtcgcg atattgcgga agcgcaccgc gcctcgacgc cgctcgagct gctcttcgat
      781 ctttgcttcg tggtcgcggt ttcgcaggca gttaccggtc tgcaccattt cgcggccgac
      841 gggcgcttcg cggacggcgt gctccgctac gggcttgcct tcttcgcgat ctggtgggcc
      901 tgggtgaact tctcctggtt cgcgtccgca ttcgacactg acgatgtggc ttaccgcgtc
      961 gtcgtcctcg tccagatcgc cggaaacctg gtcatcgccg ccggtattcc ccgcttcttc
     1021 gagcacatgg acctcaaggt gatggtggcc ggctacgtta tcatgcgcct cggcctcgtc
     1081 agcctgtggc tccgcgtggc tcgctccgac tccatcgctg cgccgacggc ccggcgatat
     1141 gcactcgggg tgaccctcgt acaggtcggc tgggcggccg cgttactcgt tccggaccag
     1201 gcccgggtgc ccgacttcat cgtgctcatc ctgatcgaga tggcggtgcc ggtctgggca
     1261 gagcgacccg gcatgaccca ctggcaccct ggccacatcg cggagcgcta cgggctcttc
     1321 acggtgatcg tgctcggcga gtccatcctt ggcgcgaccg tctcgatcca gtccgcattc
     1381 gatgccgggg aggaggtgac gagcctgatc atgattgcca cgggcgggtt gctgatcgtg
     1441 ttcgcgatgt ggtggctcta cttcgaccag ccgaccgacg gctccctgcg caggttctgg
     1501 cagggggagg cggggccgcg ggagagcccg ttcatcttcg ggtacggtca cctcttcatc
     1561 ttcgcctcgg ccgccgcagt cggaggcgga ctcgagatcg cggtcgatta cgcgacgcac
     1621 catacagaga tcagcgcgtc acaggcggcc gcgatgatcg ccgggccggt cgcgctttac
     1681 ctcgtctgta tctggctcct gcggttcacg ctccatcaga gcccgtccct gaagacggcc
     1741 ctgtatgcaa tcgcggtcgt cctggtgctg gccaccatgt tgctttcgca gccggcgttg
     1801 ccgatcgggc tgatcaccgc cggccttgtg accgcgctgg tggtcgtgcg ctggcgcatc
     1861 gagacccgcg catcaacacc ccaacccgcc gactgaggga gtccacgcaa gcgaggacgc
     1921 ggaaacgccc tggttccgat cgcctttgat cgaagccagg gcgtttcccg tcgatgtgcg
     1981 gtgtcagacg acgacggcgg ccatggtccc gagggcggcg tgggccgccg cgatccgcgc
     2041 gaccgggaca cggtaggggg agcagctcac atagtccatt ccaatctcgt ggcagaactg
     2101 gatcgagtcc gggtcgccgc catgctcgcc gcagacgccc agctccaggt tcggcttggc
     2161 cgcacggccc cgctcggccg cgatccgaat caactcgccg acgccctccc ggtcaagcac
     2221 ctggaacggg tcctggtcgt agatcccctc ggccaggtac tgcggcagga aggtgccggc
     2281 gtcgtcccgg ctcagaccgt agacggtctg ggtcaggtcg ttcgtgccga agctgaagaa
     2341 atcagcttcg gtcgcgaggt cggccccgcg caaggcagcg cggggtagct cgatcatggt
     2401 gccgacacgg taggcgaccg atcgacccgc gttcgcgaag attcgtgtcg ccacgtcgtc
     2461 gatcaccttc cgctgccggc gcagctcctc gacgtccgag acgagcggga tcatgatctc
     2521 gggccggacg tcgacgccct ccattgcgca gagcagtgcc gcccggaaga tggcccgcgc
     2581 ctgcatctcg gtgatctccg gcacggtcat cccgagccgg cagccgcgat ggccaagcat
     2641 cgggttggcc tcccgcagct cctcgatccg cgcctcgact gccgcgacgc tcatcccgat
     2701 ccggctcgcg agctgcgcga tctcgtccgt tcgctggggc aggaactcgt gcagcggagg
     2761 gtcgagcgtg cggatcgtga ccggcaaccc gtccattgcc cggaagatgc cggcgaagtc
     2821 gccgaattgg aattgctcga gcttggccag cgcccgctgc cgctccacga cgtcctcagc
     2881 caggatcaaa gtgcggacga cgtgcaggcg gccgtcgctg aagaacatgt gctccgtgcg
     2941 gcagagaccg atcccctccg cgccgagcga tcgcgcgcgc tgcgtatcgg ccacggtgtc
     3001 ggcattggcg cggacatgca gccgccgaat gtcgtcggcc cacgagagca aggtctgcgc
     3061 gaactcgtcg agatgcgcgg gaaccatcgc gaccttgccg gtgatgacgt ggccggtgct
     3121 gccgtcgatg gtcaggtcgt cgccggcccg gatcaccgtg ctcccgatgc ggatcacgcc
     3181 ggaccgctca tcaacttcga gcgcactgca accgactacc gccggtgtgc ccattccccg
     3241 ggcgacgacc gccgcgtggg aggtcatgcc gccccgggcg gtcaggatgc ccttgaccca
     3301 gcgcatgccg ggaaagtcgt ccgcagccgt ttccgggcgc acgagcaaga tcgcctcgcc
     3361 cgcctcaccg cgccgcttcg cctcggccgt ggtcaacacg accgcgccgc tcgccgcgcc
     3421 cgggctggcc ggcaacccag cggcgatcac ggcgaggtcg cgagacgggt cgatgtgcgg
     3481 atgcataagc agttcgatct gggcgggact gacccggtgg accgcctcag tccggtcaat
     3541 cgaaccggca ttggccaggt cgaccgcgat ccggacggcc gcctgcgcgg ttcgtttgcc
     3601 ggttcgtgtc tggaggatcc agagtcggcc ctgttcgacc gtgaactcaa tgtcctgcat
     3661 atcgaggaag tgggtttcaa gctggtcggc gagcgcctgc aattgcgtgc cggcatcgtg
     3721 aaagacgggg tcgccgaaca tctcgctaat cgggcgcggg gtgcgagtgc cggccacgat
     3781 gtcctcgccc tgtgcgttct ggaggaactc accgaagagg ccgggtgcgc cggtgttcgg
     3841 gttccgggtg aaggcgacgc ccgtcgcact gtccgcgccc atgttgccga agaccatcgc
     3901 ctgaatattg gcggccgtgc ccatatcgtc actcaggccg tgcatctgcc gatacgattt
     3961 cgcccgctca gtattgaagg aacggaagac cgccaggatc gcttcgcgga cttgctcgcg
     4021 cgggtcatcg ggcaatacgg tgccttgggc atcgagaatt tcgcggaaat ggatgatcaa
     4081 tcgccggagc gcgactggcg tcaattcgtg atcactcttg acgccgaggg cgaggcgggc
     4141 gcccgagagg gcgtcgtcaa agcgctcggc cggtgcgtcg aggacgacct tgccgaacat
     4201 ctggatcaga cggcgatagg cgtcgagcgc gaaccgctcg tccaccagtc ggccaagccc
     4261 ttccgcggtg gcgtcgttga ggccgacgtt gaggatcgtg tccatcatgc ccggcatcga
     4321 ggcggccgcg ccagagcgaa ccgagacgaa gaggggcttc gcagggtcgc cgagacggtg
     4381 accgagccgc ccttccgtat agcggatgcc gtattcgacc tcttcccaga gcccttcggg
     4441 gaccttgccg ccgcggcgga ggtattccgt gcaggcttcg gtcgtgacgg tgaagccggg
     4501 cggaatcggc aggccgagct tggtcatctc ggccagattg gcgcctttgc cgccgagcag
     4561 gtcacgctgg gaggcgtcgc cttcggtgaa caatcggatc cagcggccca tagtcttgtc
     4621 tcctcgtgtc gatccggttg gacggtccaa gacacgccgt cccaagcgcc tcaggtctgc
     4681 atgatagcaa cttacatgag gacttgtgaa tactttctca caacctttca tgactctgtc
     4741 cctgtggtca gattcttaca tgcaaagtgt gtatttctac ggacgtcttc gattt
//
LOCUS       DASSGX010000092         2662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1540742, whole genome shotgun sequence.
ACCESSION   DASSGX010000092 DASSGX010000000
VERSION     DASSGX010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1540742"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="VFI12_01640"
     CDS             complement(<1..638)
                     /locus_tag="VFI12_01640"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7055130.1"
                     /translation="MADATLRFDLSTAGPIASLLVPLDGSRLAEATIPIARTIQDRTG
                     AGVTLLHVLEHDAPQSVHGDPHLTTEADAIDYLMAVSERFSGQVELHVHPNPEHDVAE
                     SIAAHAVEFRTDLILIANHGRGGLRERIFGSIAQQVIRQGVAPVLLVPVPGPEVVAQP
                     FACQTMGVLVNGTSEAETALPAAATLARSLGAAIRLILAVPTPGTLPGDRAAA"
     gene            complement(642..1961)
                     /locus_tag="VFI12_01645"
     CDS             complement(642..1961)
                     /locus_tag="VFI12_01645"
                     /inference="COORDINATES: protein motif:HMM:NF001923.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HET7055131.1"
                     /translation="MARRITEGELIPPSGLNDAGIRAAATEVLEGRSEKSWYARLTPF
                     LGPAFIAAVAYVDPGNFATNIQGGAEFGYLLVWVIVASNLMAMLIQTLSAKLGIATGR
                     NLAEVCRERFPRPVVIGMWILAEGVAMATDLAEFLGAALGLYLLFGIPLFPSALITAV
                     ATFLLLGLQRHGFRPLEAVITIMVAVIAVCYLLETLLSSPNWGAIGKGAVTPRFDGSE
                     SVLLSVGILGATVMPHVIFLHSALTQDRIVTRDPKQLKRLFRFELVDIVIAMGIAGMI
                     NAAMLVMAASTFWEKGLTGIASIEEAHRTLEPLLGSAASTIFGISLLASGLSSSTVGT
                     MAGQVIMQGFIHRHIPIWLRRGLTMIPALIVAAIGLDPTRTLVISQVVLSFGIPVALI
                     PLVMFTAQRGLMGTLANHRVTTVVASAVAAIIVALNIFLLYETLIGG"
     gene            2134..>2662
                     /locus_tag="VFI12_01650"
     CDS             2134..>2662
                     /locus_tag="VFI12_01650"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7055132.1"
                     /translation="MRTDQYDALIIGGGPAGLSAAIYLARNLLSVAVFDCDRPARSDW
                     VQVNHNYLGFPGGISIIELGKRGREQAEQYGAHFVNAEVTLLDQVDGGFEVTAQGTTA
                     RGRAAILATGVTDRWVEFPGYEEFIGRSMHWCIVCDGYEMRGQRVLIVGDDEHAGVVS
                     LQMRRFTDRVTLLMHP"
BASE COUNT          468 a          873 c          920 g          401 t
ORIGIN      
        1 gctgccgccc gatcgccggg cagcgttccg ggggtcggca ccgcgaggat cagccggatc
       61 gccgcgccga gactccgcgc gagtgtcgcg gcagcgggta gcgcagtctc agcctcgctc
      121 gtgccattga ccagcacgcc catggtctgg caggcaaagg gctgcgccac gacctcgggg
      181 ccaggcacgg gcacaagcag gaccggcgcg acaccctgcc ggatcacttg ctgggcaatg
      241 ctgccgaaga tgcgctcgcg caaaccgccc cggccgtggt tggcgatcag gatcagatcg
      301 gtgcggaact cgaccgcatg cgccgcgatg ctctccgcga catcgtgctc cgggttcggg
      361 tgaacgtgca gctcgacttg gccgctgaac cgctccgaga ccgccatcaa gtagtcgatc
      421 gcgtccgcct cggtcgtcag atgcgggtcg ccatgcacgc tctgcggcgc gtcatgctcg
      481 agcacgtgca ggagcgtgac accagcgccc gtccgatcct ggatggtgcg ggcgatcggg
      541 attgtcgcct cggccaggcg ggagccatcg agcgggacca gcagcgaagc gatcggtccg
      601 gcggtgctca ggtcgaagcg cagtgtcgcg tcggccatcg tctatccccc gatcagcgtc
      661 tcgtagagca ggaagatgtt cagggccacg atgatagcgg caacggcgct ggcgacgaca
      721 gtcgtgacgc gatggttggc gagcgtgccc atcagtccgc gctgcgccgt gaacatcacc
      781 agggggatca gcgcgaccgg gatgccgaac gagagcacga cctggctgat gaccagcgtc
      841 cgggtcggat cgagcccgat cgccgcgacg atcagcgccg ggatcatggt cagcccgcgc
      901 cgcagccaga tcgggatgtg gcggtggatg aagccctgca tgatgacctg gccggccatc
      961 gtgccgacag tcgatgagga caagcccgag gcaaggagcg agatgccgaa gatcgtgctg
     1021 gcggccgagc cgagcagcgg ctcgagcgtc cggtgcgcct cctcgatcga ggcgatcccg
     1081 gtcagcccct tctcccagaa ggtcgaggcg gccatgacca gcatcgcggc gttgatcatc
     1141 ccggcgatgc ccatcgcaat cacgatatcg accagctcga accggaacag ccgcttgagc
     1201 tgcttcgggt cgcgcgtcac gatccggtcc tgggtcaggg ccgagtgcag gaagatcacg
     1261 tgcggcatga ccgtcgcgcc gaggatgccg accgacagca agacgctctc ggacccgtcg
     1321 aagcgcggcg tgacggcgcc cttgccgatc gcgccccagt tcggcgagct gagcagtgtc
     1381 tcgagcaggt agcagaccgc gatcaccgcg accatgatcg tgatcaccgc ctcgagcggg
     1441 cggaagccgt gccgctgcag gccgagcagc aggaaggtcg cgacggccgt gatcagggcc
     1501 gacgggaaga gcgggatgcc gaagagcaag tacaggccga gcgccgcgcc gaggaattcg
     1561 gccaggtcgg tcgccatcgc cacgccctcg gccaggatcc acatgccgat gacgaccggt
     1621 cgcgggaagc gctcgcggca gacctcggcc aggttcctgc cggtcgcgat gccgagcttg
     1681 gccgagagtg tctggatcag catcgccatc aggttcgagg cgacgatcac ccagacgagc
     1741 aggtagccga actcagcgcc gccctggatg ttggttgcga agttgccggg atcgacatag
     1801 gcgacggccg cgatgaaggc cggcccgagg aagggcgtca gccgcgcgta ccaggacttc
     1861 tcgctgcgtc cctccagcac ctccgtggcg gccgcccgaa tcccggcgtc gttcaggccg
     1921 gatggaggaa tcagttcgcc ttcggttatt cgtcgagcca cgtgcgagtc ctggcggtac
     1981 gtaccgcgct gcgcgcttta gtcgtatcta aattaaatgt tgagggaatc agttgtcaat
     2041 agcatcgcag tacgaagtgc agccggcaaa caccggcgat gaaacggcgc cgtcgataat
     2101 cccgtgcgat tagccggaat ggagacccgc ccgatgcgaa ccgaccagta tgacgccctg
     2161 atcatcggcg gcgggcccgc aggcctctcc gccgcaattt acctggcccg taacctgctc
     2221 tcggtcgccg tcttcgactg tgatcgcccg gcgcggtccg actgggtgca ggttaaccac
     2281 aactacctcg gcttcccggg cgggatctcg atcatcgagc ttggcaagcg cggccgggag
     2341 caggccgagc agtacggcgc gcacttcgtc aacgccgagg tgacgctact cgaccaggtc
     2401 gacggcggct tcgaagtcac ggcgcagggg acgaccgcgc ggggccgggc cgccatcctc
     2461 gcgaccggcg tgaccgaccg ctgggtcgag ttccccggct acgaggagtt catcggccgc
     2521 tcgatgcact ggtgcatcgt ctgcgacggc tacgaaatgc ggggccagcg ggtgctgatc
     2581 gtgggggatg acgagcacgc cggcgtcgtg tcgctccaga tgcgccgctt caccgaccgg
     2641 gtcacgctcc tgatgcaccc gg
//
LOCUS       DASSGX010000093         2944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_234298, whole genome shotgun sequence.
ACCESSION   DASSGX010000093 DASSGX010000000
VERSION     DASSGX010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234298"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VFI12_01655"
     CDS             complement(<1..228)
                     /locus_tag="VFI12_01655"
                     /inference="COORDINATES: protein motif:HMM:NF021560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2283 domain-containing protein"
                     /protein_id="HET7055133.1"
                     /translation="MTEPRLSYDPDANALYVRLSENDVVETLELSRSAYLDVDSGGAP
                     VGLEILNADPRLLAGLPILPEGTTLIDVLRRN"
     gene            complement(439..783)
                     /locus_tag="VFI12_01660"
     CDS             complement(439..783)
                     /locus_tag="VFI12_01660"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET7055134.1"
                     /translation="MSLQKAGSGPDGTPEVDVAEVARVWADRSAQLVDVREPDEWADG
                     HIPDSIHIPLGELARRSEELIRETPVVVLCRSGVRSLAGADELIARGFADVASFNGGI
                     LAWARAGHPVES"
     gene            complement(816..2198)
                     /locus_tag="VFI12_01665"
     CDS             complement(816..2198)
                     /locus_tag="VFI12_01665"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET7055135.1"
                     /translation="MIVDRIFTPGLAQVAYLIADDAAGEVAVIDPRRDVDAYIVWADE
                     RGYRITAILETHIHADFVSGSRELAVRTGAPLYASAIGKQEFPHTPIPDGQSVSVGSL
                     SLRAFWTPGHTPEHIAWLLFDPAQGEDPVALFSGDALFVGDVGRPDLLGEDQVQTLAT
                     KLFYTVVGRLCRLPSNVIVYPGHTAGSACGKKIGEAPYTTIGAERVGNYAFQARSRDS
                     FVQMVLSGMPPAPTYYPVLKTVNKHGATPLAELPAPAALELERVTAAAGAGAVIIDAR
                     PFEDFGAGHIPGAVAAGLGENFVAWMGWLAPYDRDIVLILPSDDHLDEAVTDLRRIGL
                     DRIAGYLAGGMAAWRASGRDVATLPQMTAPELAGQLNNHRIILDVRSDEEWQSEHIPG
                     AVHLYAGKIAQGADPDLPKDAEIRVICGSGYRSSFVASLLLQRGYRHLINVDGGMDAW
                     NQRKLPVTRS"
     gene            complement(2217..2459)
                     /locus_tag="VFI12_01670"
     CDS             complement(2217..2459)
                     /locus_tag="VFI12_01670"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HET7055136.1"
                     /translation="MIARLKRIEGQARGIQKMLEDGRDCSDVINQIAAVKAATNALSV
                     EALEAFALHCFRTAASSERPEEAVSEAVRALVRAGR"
     gene            complement(2546..2755)
                     /locus_tag="VFI12_01675"
     CDS             complement(2546..2755)
                     /locus_tag="VFI12_01675"
                     /inference="COORDINATES: protein motif:HMM:NF022577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HET7055137.1"
                     /translation="MSTRWGRISRVIGGILMMAAGLFVIGGGLGFAIAAGSLLPIATG
                     LFNICPISPFYGLPLRTCPVPARRQ"
BASE COUNT          528 a          959 c          965 g          492 t
ORIGIN      
        1 gtttcgtcgc aatacgtcga tcagggtagt gccttcaggg agaatgggga ggcccgcaag
       61 caggcgaggg tcggcgttga ggatctcaag gccgaccggt gctcccccag agtcgacgtc
      121 gagataggcc gagcgcgaaa gctccagcgt ctccactacg tcattctctg aaaggcgcac
      181 atagagtgca ttcgcatctg ggtcgtaact gagccggggc tctgtcatgt tgccttcctc
      241 agtcgcatcg cggtaatcat ccgatgatta ggggcgaccc ggttgatgca tgcgaagcgc
      301 cgatccagag tcgcccgggc gtttacgccc gggctgaatc aaacaagtca gctaaagcag
      361 gatgaacaga atgagcgggc cacgagcact ccgctcattc ttgttctatc aaccctccac
      421 tctcaacctt caaccaactc acgactcaac cgggtgaccg gcgcgggccc aggcgaggat
      481 gccgccgttg aagctggcca catcggcgaa gccgcgggcg atcagctcgt ccgcgccggc
      541 caggctgcgc accccggatc ggcagaggac gaccaccggc gtctccctga tcagctcctc
      601 cgatctccgg gccaactcgc cgagcgggat atggatcgag tccggaatat gcccgtccgc
      661 ccattcgtcc ggctcgcgga catcgaccaa ctgcgccgag cgatccgccc agacgcgggc
      721 gacttcggca acgtcaacct cgggcgtgcc atccggcccg ctccctgctt tctgcaaact
      781 catcggtgct cctcagccgg gtgattcacg aacgatcagg acctcgtgac gggtagtttg
      841 cgctggttcc aggcgtccat accaccgtcg acgttgatca gatgccgata tccgcgttgc
      901 aggaggagac tggcgacgaa gctggagcgg tagccgctgc cgcagatgac ccggatctcg
      961 gcgtctttcg gcagatccgg atccgcaccc tgggcgatct tgccggcgta gaggtgaact
     1021 gcgccgggga tgtgctcgct ctgccactcc tcgtcgctgc gaacgtcgag gatgatccgg
     1081 tggttattga gctgcccggc gagctccggc gcagtcatct gcgggagcgt cgcgacgtca
     1141 cggccactcg ctctccacgc cgccatgccg ccggcgaggt aaccggcgat ccgatccagc
     1201 ccgatccggc gaagatcggt gacggcttcg tcaaggtggt catcggaggg caggatgagg
     1261 acgatgtcgc ggtcgtacgg cgcgagccag cccatccagg cgacgaagtt ctctcccagg
     1321 ccggcggcga ccgcgcccgg gatgtgcccc gcgccaaaat cctcgaacgg ccgggcatcg
     1381 atgatcaccg cgccggcccc ggcggcagcc gtgacccgct ccagctcgag cgcggcgggc
     1441 gcgggcagct ccgcgagcgg cgtcgcgccg tgcttgttga ccgtcttgag caccgggtaa
     1501 taggtcggcg cgggcggcat gccgctcagg accatctgca cgaaggaatc gcgcgagcgg
     1561 gcctgaaaag cgtaattgcc gactcgctcg gcgccgatcg tggtgtaggg agcctcgccg
     1621 atcttcttgc cgcaggccga tccggccgta tggcccgggt agacgatgac attgctcggc
     1681 aggcggcaga gccggcccac gaccgtgtag aagagcttgg tggccagcgt ctggacctgg
     1741 tcttcgccga gcaggtcggg ccggccgaca tcgccgacga agagggcgtc gcccgagaag
     1801 agcgcgaccg ggtcctcacc ctgcgcggga tcgaacagca gccaggcgat gtgctccggc
     1861 gtgtggcccg gcgtccagaa ggcgcgcagc gagagcgatc cgactgacac cgattgcccg
     1921 tccgggatcg gcgtgtgcgg gaactcctgc ttgccgatgg cgctggcgta gagcggcgcg
     1981 ccggtgcgga cagcgagctc ccgcgagccc gacacgaagt cggcgtggat gtgtgtctcg
     2041 agaatggcag tgatccggta tccccgctca tccgcccaca cgatgtaggc atcgacatcg
     2101 cggcgggggt cgatcacggc cacttcgccg gcggcgtcat cggcgatgag gtaggcgacc
     2161 tgggcgaggc cgggggtgaa gatgcggtcg acgatcacgc gatgccgagc ctcctgttaa
     2221 cggccggccc gcaccagcgc ccggacggct tcggaaaccg cctcctcggg gcgctcggag
     2281 ctggcggcgg tgcggaagca gtgcagcgcg aacgcctcaa gcgcctcgac gctcagcgcg
     2341 ttggtcgcgg ccttgactgc cgcgatctgg tttatcacgt cagagcaatc gcgaccatcc
     2401 tccagcattt tctgaatgcc acgggcttgc ccctcgatcc gtttgagccg cgcgatcata
     2461 tccgcgcggg catcactggg gagttctgaa atacttgcca tcatgttcct cgactttcgc
     2521 ttcgggccct ggccggtgtc cggaattact gcctccgagc cggcacaggg catgtgcgca
     2581 acggcagccc gtagaacggc gagatcggac aaatgttgaa caggccggtg gcgatcggca
     2641 gcagactgcc tgctgcaatg gcgaaaccca aaccaccgcc gatgacgaag aggccggccg
     2701 ccatcatgag gatgccgcca atcactcgag agattcgacc ccaccgggtc gacattgcca
     2761 acgcaacctt cacgctcaag actccatgca gataatacgg acgaccaatc gagtaccctg
     2821 cgggggtagt gtatcagaag atgacgtgcc tttcccccaa aggggatgtt ggccggcccc
     2881 gggtatcccc gaatgctgga ctcacctgcc gacggtaatc tgccgttcgg cgcggtgctc
     2941 gatt
//
LOCUS       DASSGX010000094         5332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1105385, whole genome shotgun sequence.
ACCESSION   DASSGX010000094 DASSGX010000000
VERSION     DASSGX010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5332
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1105385"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            835..1215
                     /locus_tag="VFI12_01680"
     CDS             835..1215
                     /locus_tag="VFI12_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055138.1"
                     /translation="MDNGVVAVAVATTLISHLTPHLAEITDQVERLTREVMTEKIRAE
                     TAERERDQLQAELEQVSRELLATLTGVSDAEGSQHRDHSGHAAALNEWERDRSFTTDD
                     TEPDSWWRRALGLGPSSGPPRPRF"
     gene            1509..1724
                     /locus_tag="VFI12_01685"
     CDS             1509..1724
                     /locus_tag="VFI12_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055139.1"
                     /translation="MSSSRGCTEQYSTVIFGYEVSNSLQYWISQAMYIVDGRPLGTVV
                     IPAERRQSFVSPALLDVTPATDSHRHK"
     gene            complement(1842..2492)
                     /gene="folE"
                     /locus_tag="VFI12_01690"
     CDS             complement(1842..2492)
                     /gene="folE"
                     /locus_tag="VFI12_01690"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HET7055140.1"
                     /translation="MSIQQLAVVPNAPVSLDLVPRTMSVEQMHRFEGYAAEIFSALGL
                     DLNTPATVDTPRRFIQALFDATVGYDGDPKLVTVFETECRSGSDCRLSQVVEGPIQFY
                     ALCEHHALPFYGHAYIGYIADEHIIGISKMTRLVRLFAQRFSVQERIGQQIADTLEAM
                     LEPHGVAVYLEAHHLCMQMRGVQEPASLTRTTFWRGVYDDDPALRTEFLSLCGVGR"
     gene            complement(2626..3117)
                     /locus_tag="VFI12_01695"
     CDS             complement(2626..3117)
                     /locus_tag="VFI12_01695"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HET7055141.1"
                     /translation="MSTTHITTPALSRIKLRRAYESASPSDGTRFLVDRLWPRGLTKA
                     AVAVDAWLKEVAPSTELRKWFAHDPARWLTFEERYRRELAAHPDALTPLLDAARAGPI
                     TLVYGAKDEQHNAAVVLREFLGECLEFNGEGGQMDLVDNASFDSFPASDAPAWATGQA
                     REP"
     gene            complement(3250..3522)
                     /locus_tag="VFI12_01700"
     CDS             complement(3250..3522)
                     /locus_tag="VFI12_01700"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HET7055142.1"
                     /translation="MAESVTIQLQDGWFDPAHIAATNGHDLTITLINAGSRQHAFRID
                     QYQVNVSLAPGETQTIVIHQPTLGDFTYYSDAAGDEGMEGKLTFYI"
     gene            complement(3770..>5332)
                     /locus_tag="VFI12_01705"
     CDS             complement(3770..>5332)
                     /locus_tag="VFI12_01705"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HET7055143.1"
                     /translation="AGGLADPVNLASANDGSGRLFVVERVGMIRVIDKDNNLLPDPFL
                     DISALVKTDFLEQGLLGLAFHPDYTTNGLFYVYYSDYLTNGDTVLAEFHVSADDPNKA
                     DPASARVLMTQDKPYVNHNGGTVHFGPDGYLYWVMGDGGLAGDPYDNAQRYDVLLGKM
                     LRIDVDARDAGAYGIPADNPFADTGVVLPSDQASTKAQDGSYHPAAKREICNWGLRNP
                     WQFSFDMATGDLYIADVGQNAWEEINYLPADQTCGWNFGWDHNEGAHCYPPEATQCDK
                     LGVLPVANLNHSNGDCSITGMGVYRGQESPSLDGIYFNSDFCSGRVYGLAQDSNGTWI
                     YQSLLQTSLSVTGSGQSESGELYLTSCSCQFDRTYNPMENPGGAVWRVVETDKVPQDA
                     EIAPTPIASASPAAEAATPVPTEAAAPGTDAQGQPAIEAVDIAFQPTELTIAAGQDVT
                     VTLTNNGASMHNFSITDHNNPGVENLNISVDLAPGQTQTVTINAPAGDYYFFCSVPGH
                     EAAGMHGTIHVK"
BASE COUNT          988 a         1602 c         1753 g          989 t
ORIGIN      
        1 cggtgaccgt gacctgcagc gtcgccacac cgaccgcggc ggcagtgcct tgggacaagc
       61 cgaaacagat agccaccgcg gcgagcatcg ccagggtgag cgtccgaagg cgcatcgagt
      121 cgcgaatcat ggtgctgctc ctctccgccg cggcacaggc gtgccacgac taatcccggg
      181 ccactcgccg accggtgcga ttaccggcag cggacgagcg acccaaccct atcctgggga
      241 cagggacagc tcggggcatc cagaagatca tgtacaggga catgcaattt gcggaagtga
      301 gcttgccaat gatgagaggg gcgaccggga gaagccggtg attcgtcccg gcagagcagc
      361 gcctggtgga taatgcgtgc caccgcggag aatcgaccgg cgtccatcgt cgcgctgtgt
      421 ttccgggacg tcgcgtggat gaccgccccg cgttctggat ttgtgccggt ggtgacgcct
      481 cgctcgtagc attgcacacc atgctccggg cgtgccgttt agtatgagta ccatgggtcg
      541 ggcattgggg ccgggtgcta gcggtaacct cgcccgaaaa ggatgagcat gatggccacg
      601 ctcgccaacc agggacatat gcctgcctct gacgggttcg taaccgtgga ggaggcggca
      661 aggatggcgg aggtgtccac gcggacgatt cgccgctgga tcaccgccgg agacgtgcgc
      721 gcggaagaag ggcccgaagc gcggcgggtg tccgtttctg atgttcatcg cagggccgcg
      781 cggagccatg tgcggtcacg ccgaccgccc ggatcagacc agtccgagcc ggacatggat
      841 aatggcgtcg tcgcggtcgc ggtcgcgacc acgttgatca gtcacctcac cccgcatctt
      901 gcggagatca ccgaccaggt cgagcggctc acacgagagg tcatgaccga gaagatcagg
      961 gctgagaccg cggagcggga gcgggaccaa ctccaggcag aactggagca ggtcagccgg
     1021 gaactcctcg cgacgctcac tggcgtgtct gatgccgaag gctcccagca ccgcgatcat
     1081 tcggggcatg cagcggcgct caacgagtgg gagcgtgatc gttccttcac aacggacgat
     1141 acggaaccgg actcctggtg gcggcgcgcg ttggggctgg gaccgagttc gggtccgccg
     1201 cgtccgcggt tctgaggcag agcgctccgc ctggtcccga gcttcccgac gagatcctcg
     1261 accgcgctca tccaatgact catgcaagac tgagtcaacc cggtgcgctg cgagggttcg
     1321 ctcgctattc agcatgcgtg agttggcaac tactcggtac attggcgcat tgctgctcgg
     1381 tccgaatcct tccgggcctc tcgaaggtgg cggaggacgt tgccgccgat gatcttgcgg
     1441 acatcctcgt ctctgtaccc gcgccgcaac ggcgcatccg tcaaccgcgc gagcgagttg
     1501 aatggggagt gtcgtcctcg cgcggatgca ccgagcagta ctcgaccgtg atcttcggat
     1561 atgaggtgtc gaactccttg cagtactgga tcagccaggc gatgtacatc gtggacggtc
     1621 ggccgcttgg tacggtcgtt attccagcag agcggcggca gtcattcgtg tcgccggcac
     1681 tgctcgatgt cactccggct acggactcgc accggcacaa gtgagtagcg gtgagtcggc
     1741 cccctcaaca ctcccaagag gttcgcgaag ccgtcgattc gcatacgcac gagataaacc
     1801 gcaatcgatc tgaagaggtt tttcatctct ctccctttct gtcagcggcc cacgccgcag
     1861 aggcttagaa actcggtacg caaggccgga tcgtcgtcgt aaacgccccg ccagaatgtc
     1921 gtccgggtca gcgaggctgg ctcctggacg ccgcgcatct gcatgcagag gtgatgcgcc
     1981 tcgaggtaga ccgccacgcc gtgcggctcg agcatcgcct ccagcgtgtc ggcaatctgc
     2041 tggccgatcc gttcctgtac cgaaaagcgt tgagcgaaca agcggacaag ccgcgtcatc
     2101 ttggaaatgc cgatgatgtg ctcgtcggcg atgtacccga tgtaggcgtg tccgtagaac
     2161 ggcagggcgt ggtgctcaca cagtgcgtag aactgtatgg gtccctcgac aacctggctt
     2221 aaccggcagt ctgatccgct tcggcattcg gtctcaaaga ccgtgacgag cttcgggtcg
     2281 ccgtcatagc caacggtcgc gtcgaacagg gcctggatga agcgtcgcgg cgtatcgacg
     2341 gtggcgggcg tgttgagatc caggccaagc gccgagaata tttcagccgc gtagccctcg
     2401 aaccggtgca tctgttcgac gctcatcgtt cgcggtacca gatccaacga gaccggggca
     2461 ttggggacga cagcaagttg ttgaatggac atgaggactc ctggtcttgg taatggagtt
     2521 ggtccgctat gggcgacgct gcccacgcgc tcgagtggcg cagcggaacc ggcccgcgcc
     2581 agcagcacgt tcgacgccga cagtgtcctg cggacacgct gcgagtcacg gctcgcgggc
     2641 ttgaccagtt gcccaggctg gagcgtcgct cgcggggaac gaatcaaagg acgcgttgtc
     2701 gaccagatcc atctggccac cttcgccatt gaactcaagg cattcgccaa ggaattccct
     2761 gagcaccacg gcggcattat gttgctcgtc cttggctcca tagaccagcg tgattggccc
     2821 ggcacgcgcc gcgtcgagga gaggagtcag cgcatcggga tgggccgcga gctcccggcg
     2881 ataccgttcc tcaaacgtga gccaccgggc cggatcgtgc gcaaaccact tccgcagctc
     2941 cgtactcggc gcgacctctt ttagccacgc atcgacggct acggccgcct tcgtcaaacc
     3001 tcgcggccag agccggtcaa ccaggaaacg cgtcccatcg gagggagatg ccgactcgta
     3061 ggcgcgacgg agcttgattc gcgaaagggc aggtgtggtg atatgagtgg tcgacattgc
     3121 ttgcctcttg catgtccgtt acggtggagg agcgagtcga tgctgccagt caacgctcaa
     3181 ttcgccatgt ttgcgcgaga tttagcttgc tgatggattc ggctcgacac cgggatggct
     3241 cgtcgtggat cagatataga acgtgagctt gccctccatt ccctcgtcgc cggccgcatc
     3301 gctgtagtag gtgaagtcgc cgagcgtggg ctggtgaatc acgatcgtct gcgtctctcc
     3361 aggcgccaga ctgacgttga cctgatattg atcgatccgg aacgcgtgct gccgggaacc
     3421 cgcgttgatc agcgtgatgg tcaggtcgtg accgttcgtg gccgcgatgt gggccggatc
     3481 gaaccagccg tcctgaagtt ggatcgttac ggactccgcc acggaaatcg atcggccaac
     3541 cggtgaggag actggtgacg ccacgggtga ggaaatcggc gacgccgacg gggaggaaac
     3601 gggcgacgcc cccggcgtca ccattcccgc gctttcgctc cgcaggccgc caaccaacgc
     3661 gacggcgata gaaagcatga gcgcggcggt caacgcgcgc ttgaccatgg ctgtgccccc
     3721 ggaacggaac cggggcggca tgccgccccg gttcccgcgt gtgccatcgc tatttgacat
     3781 gtatggttcc gtgcatgccg gctgcctcat ggccgggcac gctgcagaag aagtagtagt
     3841 cgccggccgg agcattgatc gtaacggttt gcgtctgccc cggtgcgagg tcaacggaga
     3901 tgttcagatt ctcgacgccg ggattattgt ggtcggtgat cgagaagttg tgcattgacg
     3961 cgccgttgtt ggtcagggtg acggtaacgt cctggccggc ggcaatcgtg agctcggtcg
     4021 gctgaaacgc gatgtcgact gcctcaatcg ccggttgtcc ttgcgcgtca gtacccggag
     4081 cagcggcctc ggtgggaacg ggagttgccg cttccgccgc gggcgatgcc gacgcgatcg
     4141 gcgtgggcgc gatttccgca tcttggggga ccttgtcggt ctcaaccacg cgccaaacgg
     4201 cgccgcccgg attctccatc gggttgtagg tgcgatcaaa ctggcaggag cagctcgtga
     4261 gatagagctc acccgattcg ctttgcccgg atcccgtcac gctcagcgat gtttgcagca
     4321 gcgactggta gatccaggtg ccatttgagt cctgcgcgag cccgtagact cgacctgaac
     4381 agaagtcgga attgaagtag atgccgtcga gcgacggtga ttcctggcca cgatagacac
     4441 ccatacccgt gatcgagcaa tcaccattgg aatggttcag gttggcaacc ggcagaacac
     4501 cgagcttgtc gcactgcgtg gcttctggcg gatagcagtg ggcgccttcg ttgtggtccc
     4561 agccgaagtt ccatccacac gtctgatcag cgggaagata gttgatctct tcccaggcat
     4621 tctggccgac gtcggcgatg tacaagtcgc cggtcgccat gtcgaagctg aactgccagg
     4681 gattgcgcag gccccagttg cagatctcgc gcttggcggc ggggtggtag gagccgtcct
     4741 gagccttggt gctcgcctgg tcagagggaa ggacaacgcc ggtatccgcg aatgggttgt
     4801 cggcgggaat gccataggcg ccggcgtcac gcgcgtcgac gtcgatgcgc agcatcttac
     4861 cgagcagaac atcgtaccgc tgggcgttgt catacggatc gccggcgagg ccgccgtcgc
     4921 ccatcaccca gtagaggtag ccgtcggggc cgaagtgcac cgtaccgcca ttgtggttga
     4981 cgtacggctt gtcctgcgtc atcaacacgc gcgcgctcgc gggatcggcc ttgttcggat
     5041 cgtccgcgga aacgtgaaac tcggccagga ccgtgtcacc gttggtcagg tagtcggagt
     5101 agtacacgta aaagaggccg ttcgtggtgt agtccggatg gaacgccagc ccgagcaggc
     5161 cctgctcgag gaagtcggtc ttgaccagcg ccgagatatc gaggaacgga tccggcagaa
     5221 ggttgttgtc cttatcgatc acccggatca tgccgacgcg ctcgacaacg aacagccggc
     5281 cgctgccatc attggcgctg gcgagattga ccggatcggc caacccgccc gc
//
LOCUS       DASSGX010000095         2703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_670191, whole genome shotgun sequence.
ACCESSION   DASSGX010000095 DASSGX010000000
VERSION     DASSGX010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670191"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VFI12_01710"
     CDS             complement(<1..206)
                     /locus_tag="VFI12_01710"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HET7055144.1"
                     /translation="MSDTGRGVAAQSATADREIVISRVISAPRELVFEAFTEVRHLSR
                     WWGPEGFSTTTRAFEFRVGGEWDFV"
     gene            complement(210..551)
                     /locus_tag="VFI12_01715"
     CDS             complement(210..551)
                     /locus_tag="VFI12_01715"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET7055145.1"
                     /translation="MARAATTSDVFNAIAEPQRREILVLLRAGERPVSELAQELGMTQ
                     PGASKHLRVLREVGLVRDRKAGKHRLYGLDARGLRPVHEWTGGFERFWNESFDRLDEY
                     VQELIQVRQEE"
     gene            807..1592
                     /locus_tag="VFI12_01720"
     CDS             807..1592
                     /locus_tag="VFI12_01720"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET7055146.1"
                     /translation="MKIEPGRIRLVTFDLYDTLIELDPKRWERLSAVLTARGITHDLN
                     ALKANDVIAEDYYTELNTIQPIRERDSAIQDQIRVEYIRRWLLASGIDVGDQTAADIR
                     NAYRAEYETRARQSIPGMVDGYRVFPDVNRTMRRLREAGILSAVISNADSDVTDFCAR
                     LQFAHEMDLIVTSALVGYEKPDVRTFQAALEPFDVDGPDAIHIGDQPRSDVAGALATG
                     MRAALIDRYRRHTQEAHSVPIFHTLDEFTDAVLAINAAAGVTA"
     gene            1589..2242
                     /locus_tag="VFI12_01725"
     CDS             1589..2242
                     /locus_tag="VFI12_01725"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HET7055147.1"
                     /translation="MIELLSVNVALSEVIGVRRGRPVLSGILKRPVQSETVEVGPTNI
                     AGDGQADLRAHGGPDKAVYAYTADHFPWWTAQMTPDQPYGPGALGENLTIAGIDETGL
                     RIGDILQWGDAVLQVSQPRYPCYKLAMATHRPTIVKRFLESGRSGVYLRVLETGCAPV
                     RGTICIRERDVAGVTVREAALAASHPTEPERLIEIAAHPALAPRWAEKLRHIAITPG"
     gene            2292..2543
                     /locus_tag="VFI12_01730"
     CDS             2292..2543
                     /locus_tag="VFI12_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055148.1"
                     /translation="MFEDRGNVIGIALLILCGLSAGTMLGYINAGEMARFRGPGWVGL
                     VIIVAFIGLTIFGVVQMARGKHLGNDIRGPRRRWWRRNQ"
BASE COUNT          467 a          957 c          819 g          460 t
ORIGIN      
        1 acgaagtccc actccccgcc gacgcggaac tcgaacgccc gcgtggtggt gctgaacccc
       61 tccggtcccc accaccgcga caggtgccgc acctcggtga acgcctcgaa caccagctcc
      121 cgtggggcac tgatgacccg ggagatcacg atctcgcggt cggccgtcgc cgactgcgcc
      181 gccactcctc gccccgtgtc gctcatctgt tattcctcct gccttacctg tatgagttcc
      241 tgcacatact cgtccagccg gtcgaagctc tcgttccaga accgctcgaa cccgccggtc
      301 cactcatgaa ccggtcgcag cccgcgggcg tccaggccgt acagacggtg cttgcctgcc
      361 ttgcggtccc gcaccagtcc gacctcccgg agcacccgca ggtgttttga cgcccccggc
      421 tgggtcatcc ccagctcctg cgccagctcg ctcaccggtc gctcacccgc ccgcagcagc
      481 accaggatct cccggcgctg cggctcagcg attgcgttga agacatccga cgtcgttgct
      541 gctcgtgcca tggcgcaatc atattcctat atcggaatgt gtcaaggcca gcggaatcag
      601 cgctcggact cggtccccgt cctcgccgtt ccggatcccc gcaagcgccg ccccagccgg
      661 tgcgtcaacg ccagcatttg atcagacgcg ctttggggta tcgaacttcc tcgaaaagga
      721 gcgccccttc acgtcgcccc gccgaggacc gctcgcagat cggaacatcg ctcgatgggc
      781 cggctgaagt aggtatgatt ccggttatga agattgaacc cggacggatt cgcctcgtca
      841 cgttcgacct ctacgacacg ctgatcgagc tcgatcccaa gcgctgggag cggctctcgg
      901 ccgtcctgac cgcccgcggc atcacgcatg acctgaatgc gctcaaggcc aacgatgtca
      961 tcgccgagga ctactacact gagctcaaca cgatccagcc gatccgcgag cgggattccg
     1021 ccatccagga ccagatccgg gtcgaatata tccggcgctg gctcctcgcc tccggaatcg
     1081 atgttggaga ccagacggca gccgacatcc ggaacgccta ccgggccgaa tacgagaccc
     1141 gcgcccgcca gtcgatcccc gggatggtcg atggctaccg ggtcttcccg gacgtgaacc
     1201 ggacgatgcg ccgcctgcgc gaggccggca ttctcagcgc cgtcatctcg aacgcggact
     1261 ccgacgtgac cgacttctgc gcccggctcc agttcgcgca tgagatggac ctgatcgtta
     1321 cctccgcgtt ggtcgggtat gaaaagcccg acgtgcgcac cttccaggcc gcgctcgagc
     1381 cattcgacgt tgacgggccg gacgcgatcc atatcggtga tcagccgcgc tccgatgtcg
     1441 ccggcgctct ggccaccggc atgcgggcgg ccctgatcga ccggtatcgt cgccacaccc
     1501 aggaggcgca ctcggtcccg atcttccaca cgctcgacga gttcacggat gccgtcctcg
     1561 cgattaacgc agccgccgga gtcacggcgt gatcgaactc ctctcggtca atgtcgcgct
     1621 gtcggaggtg atcggggtcc gcagggggcg gccggtcctg agcggtatcc tgaagcgccc
     1681 ggttcaatct gaaaccgtcg aggtcgggcc taccaacatc gccggcgacg gtcaggccga
     1741 tctccgtgcg cacggcgggc cagacaaggc cgtctatgcc tacactgccg accatttccc
     1801 gtggtggacc gcgcaaatga cgccggacca gccgtatggg ccgggtgcac tcggcgagaa
     1861 cctgaccatc gccgggatcg atgaaaccgg gcttcgcatc ggtgacatcc tgcagtgggg
     1921 cgacgcagtg cttcaggtca gccagccccg ttatccctgc tacaagctgg cgatggccac
     1981 gcaccggccg acgatcgtca agcgcttcct cgaatcgggg cgaagcggcg tgtatctgcg
     2041 ggtgctggaa acgggctgcg cgccggtccg cggcacgatc tgcatccgcg agcgcgacgt
     2101 ggccggggtc acggtccggg aggcggcgtt ggcggcctca cacccgaccg aacccgagcg
     2161 gctgatcgag atcgcggcgc acccggcgct cgctccgcgc tgggcagaga agctccgtca
     2221 catcgccatc acgccgggct gatttgccct ccgaagtatc atcaccgcat ggactgacga
     2281 aggagcacgg cgtgttcgaa gatcgcggca atgtgatagg gatcgcgctg ctgatcctct
     2341 gcggcctttc ggccgggact atgctcggct acatcaacgc cggcgagatg gcccgattcc
     2401 gcggtccggg ctgggtgggg ttggtgatca tcgtcgcctt catcggcctg accatcttcg
     2461 gcgtcgtgca aatggcgcgg ggcaagcacc tcggcaatga catccgcggc ccccgccgcc
     2521 gctggtggcg ccgcaaccag taacatgggg cgcacctgtc ccccatattc acgttgcacg
     2581 acgccgaact cacggccaat gcgctacgcc gatgtcacta tccatcgccc ggggaaaaac
     2641 cccgggctac atcaactgcg ccagctggag cgagtttcag aagaagtgcc accagcccaa
     2701 tgg
//
LOCUS       DASSGX010000096         2035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_452876, whole genome shotgun sequence.
ACCESSION   DASSGX010000096 DASSGX010000000
VERSION     DASSGX010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452876"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..603)
                     /locus_tag="VFI12_01735"
     CDS             complement(154..603)
                     /locus_tag="VFI12_01735"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7055149.1"
                     /translation="MNRRSMIAMAGMAGFAWVPAARAEDATPTVVSQNESIAREYIER
                     VWSQGDASAIDTLIAENYLPPNASDAPGREAFRARWETGWGALKSALPDLRYSVEVAI
                     GSGDFVSLRGAISGTTTSGDAIRTWYLSMLEIHDGRITTEWALVNRG"
     gene            925..>2035
                     /locus_tag="VFI12_01740"
     CDS             925..>2035
                     /locus_tag="VFI12_01740"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET7055150.1"
                     /translation="MSDLRAFFGPNAGYVLELYERYAQDPASISPADRAYFDSLDPAA
                     LRALTASPAAVGTNGAGTVQAAAIDEIVAAAALAQAVREYGHLNAKIDPLGSSLPSAP
                     ELDPAFHGLSDADMTQLPASVVGGPASDGAANAAAAMRKLREIYSGAVGYDFDHIQVA
                     AERLWLQNAVESGQFSAPLEPATRRALLRRLTQVESFERFLHQTYLGQKRFSVEGTDV
                     VVPMLDEFVHSAAATGTREVVIGMAHRGRLNVLTHILGKPYAAIIQAFEGKKKSKAVG
                     GDPSNVNDPSEDFSGDVKYHLGARLLRGEHGMAVEVPMVLAPNPSHLEQVNPVIEGMV
                     RSSQDITDRPGEPERDPKASVAIAIHGDAAFPGQGV"
BASE COUNT          339 a          718 c          634 g          344 t
ORIGIN      
        1 ctacctgacg atggacatca tgtcgatggg cggcgggggc atggccacac cgatggcgtc
       61 gcccgcttca taaccagctc cgcacacccg cagcaacgac cggcgcgtcc cgcacgcgcc
      121 ggtcgttgcg tgtcggctcg gcagttggtc atctcacccg cggttcacga gggcccattc
      181 agtagtgatg cgaccgtcat gaatctcgag catgctcagg taccaggtac gaatcgcatc
      241 gccactggtc gtcgttccac tgatcgcgcc gcgcagagag acgaaatcgc cagacccaat
      301 cgcgacctct acggaatatc ggagatctgg caatgcggac ttcagtgcgc cccagccggt
      361 ctcccaccgc gcccggaatg cttcgcgccc gggtgcgtcc gaggcattcg gcgggagata
      421 gttctcggcg atcaacgtgt cgatcgccga cgcatctccc tggctccaaa cgcgctcgat
      481 gtactctctg gcaatcgatt cattttgcga tacgacagtc ggggtcgcgt cttcggcgcg
      541 agcggccggg acccaggcga atccagccat tccagccatt gcgatcatcg agcgtcggtt
      601 catggtcgct cctccttgtc ggcgcaagtc tacgagaggc gggcgcgcaa atggtccgat
      661 cgacctgaaa cgatttagtc gatttcgtgc tccggaggtg tctgggaagg tcaaatcggc
      721 cgccggctgt gctatcctga cggtcacgcg cggcctggat ccgagcgttt cgacgagcgc
      781 ccgctcgttt ggcgatcacc agccggggct ccccgcctcg ggatcgccgg caagctgctc
      841 cggacgccgt tttcggtccg gttccgcgag tgccgccccg gcagcgtggc cagggatacc
      901 aggaatggaa tggacaggat ttcgatgagc gacctacgcg cgtttttcgg gccgaatgcc
      961 ggctacgtgc tggagctcta cgagcgatac gcccaggacc cggcctccat ctcgcccgcc
     1021 gaccgagcgt acttcgatag cctcgatccc gccgccttgc gcgcgctcac cgcgagcccg
     1081 gccgcagtcg gaaccaacgg cgccggcacg gtccaggccg ccgcgatcga cgagatcgtc
     1141 gccgccgccg cgctcgccca ggccgtccgc gaatacggtc acctgaacgc gaagatcgac
     1201 ccgctcggct cgtcgttgcc cagcgccccc gagctcgatc cggcgttcca tggcctgtcc
     1261 gacgcggata tgacccagct cccggccagc gtcgtcggcg gtcccgcttc cgacggcgca
     1321 gcgaatgccg ctgccgcaat gcggaagctg cgcgagatct attccggcgc ggtcggctac
     1381 gacttcgacc atatccaggt cgcagccgag cgtctgtggc tgcagaacgc ggtcgaatcc
     1441 ggccagttca gcgccccgct tgagccggcc acacgccgcg cgctgctccg tcgcctgacg
     1501 caggtcgaga gcttcgagcg cttcctgcac cagacctatc tcggccagaa acgcttttcg
     1561 gtcgagggca ccgacgtcgt cgtgccgatg ctggacgagt tcgtccactc ggccgctgcg
     1621 accggcacgc gcgaagtcgt gatcggcatg gcccatcgcg gccgcctgaa cgtcctgacc
     1681 catatcctcg gcaagcccta cgccgccatc atccaggcgt tcgagggcaa gaagaaatcg
     1741 aaggcggtcg gtggcgatcc atccaacgtc aacgatccgt ccgaggactt ctcgggcgac
     1801 gtcaagtacc acctcggcgc ccgcctgctc cgcggcgagc acgggatggc cgtcgaggtc
     1861 ccgatggtcc tcgcgccgaa cccgagccat ctcgagcagg tcaacccggt catcgagggc
     1921 atggtccgct cctcgcagga catcaccgat cgcccgggtg aaccggagcg cgatcccaaa
     1981 gccagcgtgg cgatcgccat ccacggcgat gccgccttcc cgggtcaggg cgtgg
//
LOCUS       DASSGX010000097         2273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_235427, whole genome shotgun sequence.
ACCESSION   DASSGX010000097 DASSGX010000000
VERSION     DASSGX010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235427"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1523)
                     /locus_tag="VFI12_01745"
     CDS             complement(<1..1523)
                     /locus_tag="VFI12_01745"
                     /inference="COORDINATES: protein motif:HMM:NF037144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HET7055151.1"
                     /translation="MPSLLIGRSVRPVFVGLMSGLLIAGASIAPAFRSAAPVAASTYI
                     REAGSNRYSTAARVSRLHFAAGVSVVYVVNGDRPAEGLVAAAAAVKHGPVLLVKRDHV
                     PSVTRNELERLRPDRIVIVGPAGQVSTAVRRSLRAIAPVVRRTGHSRYGTAVDVSQAR
                     FNAGAKMAYIANPNKAAWALGAAPAAHGRGPILFSRAGSLPASTATELRRLNPDRIII
                     AGGTDVISAAVADRLATIATVRRESGPTQYSTVAKTSRRHFTSRVSTVWVVSGTALPY
                     AMGAGTATKGQGPVLLVKHDSIPPSTRREILRLTPTKIIVIGGTDQVSSAVARALGDH
                     APIAAADTMSVAEDCSRLFWVLRNDSDPDGDHLSMTSITNQPGHGTASIVRNGRAISY
                     RPDASYTGPDTLTYRVGDGRSGTDTATVSVTVAMAGDHDGDGISDGCDAFALDPANGS
                     GAAASFTLDFDGPVSDATLLQDLGFDGVMYNANGPYTSLYDAANVDVSGGRLTINAVP
                     "
     gene            complement(1625..2263)
                     /locus_tag="VFI12_01750"
     CDS             complement(1625..2263)
                     /locus_tag="VFI12_01750"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET7055152.1"
                     /translation="MRSVESSLQRLGVDRVEMLFIHDPDTHHREALEGAYRALDRLRG
                     DGSVQAIGVGMNESAPLARFAREADFDCFLLAGRYTLLNQTGLRDLLPAAAERGMSVN
                     IGGVFNSGILADPRPGAAFDYHAVGGSSEPLKRALRMQEVCERHGVPLAAAAMQFPLA
                     HPAVGTVLVGVRSVAELEENVHNFTRPIPGDLWRELRQQGLVPVGVPLPADA"
BASE COUNT          373 a          763 c          790 g          347 t
ORIGIN      
        1 gggaccgcat tgatggtgag cctgccaccg ctgacatcga cgttggcggc gtcgtacagc
       61 gacgtgtacg gcccattggc gttgtacatc acgccgtcga agccgaggtc ctggagcagc
      121 gtggcgtcgc tgaccggtcc gtcgaagtcg agcgtgaacg aagccgcggc tccgctgcca
      181 ttggccggat cgagtgcgaa ggcatcgcaa ccgtcgctga tgccatcgcc atcgtgatcg
      241 ccggccatcg ccacagtgac cgatacggtg gcggtgtccg tcccgctgcg cccgtcaccc
      301 acacggtagg tcagcgtatc cgggccggtg tacgaagcgt caggacggta gctgatggcc
      361 cggccgttgc ggacgatcga cgccgttcca tgacccggct ggttggtgat gctcgtcatg
      421 ctcaggtgat cgccatccgg atcgctatcg ttgcgcagca cccagaacag gcgactgcag
      481 tcctcggcca cggacatggt gtccgccgcg gcaatcggcg cgtggtcacc gagcgcccgc
      541 gccaccgccg acgaaacctg atcggtcccg ccgatgacga tgatctttgt cggcgtcagc
      601 cgcaggattt cgcgccgggt gctgggcggg atcgagtcat gcttgacgag cagcaccggg
      661 ccctggccct tggtggcagt ccccgcgccc atcgcatacg gcagcgccgt gccgctcacc
      721 acccagacgg tggagacccg cgaagtgaag tgacgccgac tggtcttggc aaccgtcgag
      781 tattgcgttg ggccggactc gcgccgcacg gtggcgatgg tggccagccg atccgcgacg
      841 gccgccgaga tgacatccgt gccgccggcg atgatgatgc gatcggggtt gagccggcga
      901 agctccgtag cggtcgacgc cggtaggctg cccgcgcgag agaacaggat cggtccgcgc
      961 ccgtgggccg cgggtgcggc tcccagtgcc caggcggcct tgttcgggtt ggcgatgtac
     1021 gccatcttgg cgcccgcgtt gaatcgcgcc tgactgacgt caaccgccgt gccgtagcgc
     1081 gaatggccag tgcgccgcac gaccggcgcg atcgcccgca gagagcgccg gacggcggtt
     1141 gacacctgtc cggccggacc gacgatcacg atgcggtcgg gccgcaggcg ctcgagctcg
     1201 ttgcgcgtca cgctcggaac gtgatctcgc ttcaccagca ggaccggtcc atgcttgacc
     1261 gcggcagccg ccgcgacgag tccctcggcg ggacgatcac cgttgaccac gtagacgacc
     1321 gatactccgg ccgcgaaatg cagccgactg acccgcgccg cggtcgagta ccgattcgac
     1381 ccggcctcgc ggatgtaggt cgacgcggca accggcgccg cggatcgaaa tgcgggtgcg
     1441 atcgaggctc cggcgataag aaggccggac atcagaccaa cgaaaacggg ccgcactgaa
     1501 cggccaatga gcaggctggg cacgattact ctcctccccg gatttggcag ctcgcggacg
     1561 atacccgaat gcgggatgct gtgtcgagac tttcgtacga cggcgccagg caggtttcgg
     1621 ctggtcaggc gtcagcgggc aacgggacac cgaccggcac gagcccttgc tggcgcagct
     1681 cccgccacag gtcgccggga atcggacggg tgaagttgtg cacgttctcc tcgagctcgg
     1741 cgacggaccg caccccgacc aggacggtgc cgacggccgg atgggccagc gggaactgca
     1801 tcgccgcggc ggccagcggc acgccatggc gttcgcacac ctcctgcatg cgaagcgcgc
     1861 gcttcagcgg ctcgctgctg ccgccgacgg cgtggtagtc gaaggccgct cccggtcgcg
     1921 gatcggccag gatgccgctg ttgaagaccc cgccgatgtt gacgctcatg cctcgctcgg
     1981 ctgcggcggg caggaggtcg cgcaggccgg tctgattgag gagcgtgtat cggcccgcca
     2041 gcaggaagca gtcgaaatcg gcctcgcggg cgaagcgggc cagcggcgcc gattcgttca
     2101 tgccgacgcc gatggcctgc accgaaccgt cgccgcgcag ccgatcgagg gcccgatagg
     2161 cgccctccaa tgcctcgcga tgatgcgtat cggggtcgtg gatgaagagc atctcgaccc
     2221 gatcgactcc caagcgctgg agactcgact cgacggaccg catcacgccg tcg
//
LOCUS       DASSGX010000098         2510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_997574, whole genome shotgun sequence.
ACCESSION   DASSGX010000098 DASSGX010000000
VERSION     DASSGX010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997574"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2510)
                     /locus_tag="VFI12_01755"
     CDS             complement(<1..>2510)
                     /locus_tag="VFI12_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055153.1"
                     /translation="EVAERLDAAGEKMRRGFLDWLAVRGEAARPARMPVVFKLADAAR
                     EAVLASEPVRLQADAGGTPVVFETETDVRVVPGRLDVIVGVDAEKDEFYLPSPGLSDL
                     QPREPMPTQWQLKSFASAGATTVQLDPDSGLSPEMNIETGGEQYRIIQADKGIVTIDP
                     GLAHDLPAQTTLRQVTTFTPFENGARNRQEHSLSLGHMDLLNVEAAATIEIVGATGLS
                     NMSWQYWGKVDPNDEVGWQPLTPAVNQPAKNAVLLTKPKGAVEPIEVDGKNSRWIRAL
                     TKTVGASQQPFMTDELSIRVNAEGCDSKMPCPPKVPIASPTAEAMANTTPLVLDSAFF
                     PVGKEPRQFDAFYLGSQEAFSKKGAKVQLCFEMADRTFSALAAVREGTFANLVLAGVA
                     KDRTLHLHSFNPTTGVIGKFRERDSLLPPLPGYLGNAEAGNAVALDEQPRWRLPVWSE
                     SDFPPFAGLLVGVSAGDAIWVWHEHSIFQKSSGWIDFGTLPSIGSATTAVVDGLVYLS
                     GISPTIAALRDGHLFLRDWPNGAQWNEVDTVQGSNDVTLTSIVPVLVENHSGQLVTSA
                     AAGMLGISDNKKLFHVTTAGVCTPVLSAFDFDTEIRPVAIEVGGDRIVAAVTESDPPE
                     LVAHHDTAGTEQVTLDNDTEVLAALEAVLIGGTFHVLATIRDGAGGRLATWAPFAAGL
                     EASLFESPVAEGGGLVEGAPIAVNRFVVIPGARADILISEFDPSVRLVKSADVETGVV
                     VPDSIPALAVNDLMVRLDAGEPVSRAITQAGLTKDEEVFYPIASDFRSGTTGLDAYKL
                     SATFTGTFADPDLTLDSADHETAEGDWLWIDGEFYEVD"
BASE COUNT          471 a          796 c          765 g          478 t
ORIGIN      
        1 aatcgacctc gtagaactcg ccgtcgatcc atagccagtc gccctctgcc gtctcgtgat
       61 cggctgaatc caaggtcagg tcgggatcag caaaagtgcc ggtgaacgtg gccgacagct
      121 tgtaggcgtc gaggccggtc gtccctgagc gaaaatctga agcgattgga tagaagactt
      181 cttcatcctt cgtgagcccg gcctgcgtta tagcccggct gaccggttct ccagcgtcca
      241 accgaaccat gaggtcgttg acggccaacg ccgggatgga atccggcacc acgactccgg
      301 tctcgacatc ggcgctcttc accagtctga cggacggatc gaactcgctg atgaggatgt
      361 cggcgcgcgc gcccggaatc acgacgaagc gattgacggc aattggcgca ccctcgacga
      421 gcccgccgcc ttcggccacc ggcgattcga atagtgacgc ctcgaggccc gcggcaaacg
      481 gcgcccacgt cgcaaggcgg ccaccggcgc cgtcgcgaat cgtcgcgagc acgtggaacg
      541 ttccgccaat cagcacggcc tcgagcgccg caaggacctc ggtgtcgttg tcgagcgtca
      601 cctgctccgt acccgccgtg tcgtgatggg ccacgagctc tggcggatcg gactctgtca
      661 cagcagcgac gatccggtcg ccgccgacct cgatggctac cggacgaatc tccgtgtcga
      721 agtcgaaggc cgaaagcacg ggcgtgcaga cgccggccgt cgtgacatgg aacagcttct
      781 tgttatcgga gatgccgagc atgccggccg cggcggacgt gacgagctgg ccggaatggt
      841 tttcgacgag gaccggaacg atcgatgtaa gcgtgacgtc attggagccc tgaacggtgt
      901 cgacctcgtt ccactgcgca ccgttcggcc agtcgcgcag gaacaggtgg ccgtctcgca
      961 gggctgcaat cgtcggcgat atgcccgaga ggtacaccag accatccacg accgccgtag
     1021 ttgccgatcc gatagatggc agcgtaccga agtcgatcca tccgctgctc ttctgaaaaa
     1081 ttgaatgctc gtgccatacc cagatggcgt ctccagcgga gacacccacc agtaatccgg
     1141 cgaacggtgg gaaatcggat tcagaccaca cgggcagtcg ccagcgcggc tgctcgtcga
     1201 gcgcgacggc gtttcctgct tccgcgttgc cgagataacc cggcagaggc ggtaacagcg
     1261 aatcgcgttc gcgaaacttg ccgatcactc cggtcgtcgg gttgaatgaa tgcagatgca
     1321 gtgtccggtc cttcgccacg ccggcaagca ccaggttcgc gaacgttccc tcgcgcacag
     1381 ccgcgagcgc ggagaacgtt cgatcggcca tctcgaagca gagctggacc ttcgcgccct
     1441 tcttcgagaa agcttcctga cttccgagat aaaacgcatc gaactgccgt ggttccttgc
     1501 caaccgggaa aaatgcactg tcgagaacga gcggcgttgt gttcgccatc gcctccgcgg
     1561 tcggtgaagc gatcggtacc ttcggtggac agggcatctt gctgtcgcag ccctcggcat
     1621 tgacacgaat gctcagctca tcggtcataa agggctgctg gctcgcgccg accgttttgg
     1681 taagggcgcg aatccagcga ctatttttcc cgtcgacttc tattggttca acggcgcctt
     1741 tcggtttggt gaggaggact gcgttcttgg ccggttggtt aacagcaggg gttagcggtt
     1801 gccatcccac ctcatcgttc gggtcgacct ttccccagta ttgccaggac atattgctca
     1861 ggcctgtcgc gccaacaatt tcgatcgtcg cagccgcctc gacgttgagc aggtccatgt
     1921 gacctaaaga caaggaatgt tcttggcgat tgcgcgcgcc gttttcgaac ggcgtgaatg
     1981 tggtgacctg ccggagcgtc gtttgtgcgg gaaggtcatg cgctaacccg gggtcgatcg
     2041 tcacgatccc tttgtcggct tgaatgatcc ggtattgctc accgcccgtc tcgatattca
     2101 tctcggggga cagtcccgag tccggatcaa gctggaccgt ggtggcgccg gcagacgcaa
     2161 agcttttcag ttgccactgc gtcggcatgg gctcgcgggg ctgcagatcg ctcaaccccg
     2221 gcgacggaag atagaactcg tccttctccg catcgacccc cacgatgaca tcgaggcgac
     2281 caggcacgac acggacgtcg gtttcggttt caaacaccac cggcgtcccg ccggcatccg
     2341 cctgcaagcg caccggttcc gatgcgagga ccgcctctcg cgcggcgtcg gccagcttga
     2401 agacaaccgg cattcgggcc ggccttgccg cttcgcctcg cacggcgagc caatcgagga
     2461 agcctcggcg catcttctcc ccggcggcat cgagacgctc ggccacttcc
//
LOCUS       DASSGX010000099         3950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_345653, whole genome shotgun sequence.
ACCESSION   DASSGX010000099 DASSGX010000000
VERSION     DASSGX010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345653"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2226)
                     /locus_tag="VFI12_01760"
     CDS             complement(<1..2226)
                     /locus_tag="VFI12_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055154.1"
                     /translation="MNTSNFVKNEGTIQVTGANNSAGIVANTGLTGEILNTGTITIDE
                     AFTPTDTDSDGDLDGPFAQGSVRFGIHVLGAHTGPVTNSGKIAVEGNQSGGIVLEGPL
                     TGTLSNSNDITILGNDSVGIETGDVSGDVILSKGTIQVQGANAVGVAINGDVGGKFVF
                     QGTVQTTGYRYTSPPSDTSKLDSDDLLQAGSSIIIGGNVAGGILFDARPADTSTTDTD
                     EDDDGIPDANETTATVTNLSGAPAVLIGSATQATNIGAVAGSGGQGLVLKGSITSNGV
                     YKTVAGQGVVIGGLGNAVTIAGGMTNAGVIATAAVEANATALRIGAGASVPQIVNSGS
                     ITATGGGTATTASRAIAIDAGATVTTIKNSGTIGAGRAGSDGTATAIVDNAGTVSLIE
                     NSGSIFVANASTLGDKAIAFDLRANGGGATIRQLAVTSGAGPQIGGQILFGSGNDTLD
                     VADGTVTGAAKFGAGNNALALSGDAIMNGAVQFGAGSDSVQLGGTSKLFGGIDFGGGA
                     DVLTLAGTAAFTGQLTGTAGLAVTLGAGTSLTATNTGTVSLASLSAGDGSTLGVTIDS
                     STDTGTLFDVTGTANFGAGTTIDVNLVSLGDVAGTYKVLQAGTLTGASNLTSSVESLP
                     FIFDSSIDTSTANELSVVIRQKSAEELGINSSEAGILNALLEVADADQGIAGVFLGID
                     NSQSLQEALQQVLPDHAGGAFETATKGSRLLGRTLSDPRAPLVRSGTLGFWAQQVAWG
                     GS"
     gene            2180..2569
                     /locus_tag="VFI12_01765"
     CDS             2180..2569
                     /locus_tag="VFI12_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055155.1"
                     /translation="MTWIVPSFFTKLLVFIVTAAPLVGLTDPVLLIRMSPDALVVCGV
                     VTAVEMTVSAWAGNAIVAAAIAPKPVVASRNRIKSPFVSPLQRCGYLGWTQTSPDVFA
                     PGQMPAEIEAGFGCRRARYPPRNDGGL"
     gene            2676..3569
                     /locus_tag="VFI12_01770"
     CDS             2676..3569
                     /locus_tag="VFI12_01770"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HET7055156.1"
                     /translation="MDPILQTIAPVTHDLGGFKVHRTLPGKERTMVGPFIFFDQMGPA
                     RLGPGEGIDVRPHPHINLATVTFLFAGAIDHRDSLGTVQRIEPGAVNLMSAGRGISHS
                     ERSPADERSAGAQLDGIQTWLALPRDKEEMLPDFEHVAADRLPELDSDGVRLKLVMGD
                     WEGASSPVTQHSPTIYAAIHLAPGAALVINHEADERALYLLEGDATVDGVPLLAQNLA
                     LLAPGHLPLLSSSGGARLMLCGGAPMDGERHVWWNFVSSSRDRINEAKRAWRSGEFAL
                     PPADHDEFIPLPEVPLTVSYP"
     gene            3566..>3950
                     /locus_tag="VFI12_01775"
     CDS             3566..>3950
                     /locus_tag="VFI12_01775"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7055157.1"
                     /translation="MTEFRRVGLSTGITLNVAFAGAEDAPAVILLHGFPESHRTWREV
                     APRLEDSFRLIMPDQRGFAGSDRPQEVEAYKTDTLINDIFALADTLGLESFALVGHDW
                     GGAIAWVAALQHPYRVKRLVIVNAPH"
BASE COUNT          591 a         1307 c         1327 g          725 t
ORIGIN      
        1 gctcccgccc caggcgacct gctgcgccca gaagccgagc gtcccgctgc gaacgagcgg
       61 cgcgcgcggg tcggagagcg tgcggccgag aaggcgcgat cccttggtcg cggtctcgaa
      121 ggctccgccc gcatggtccg gaaggacctg ttgcaaggcc tcctggagac tctggctatt
      181 gtcgatcccg aggaagaccc cggcgatccc ctgatccgca tcggccactt cgaggagggc
      241 gttgaggatg ccggcttccg aggagttgat gccgagctct tccgcgctct tctggcgaat
      301 gacgacagag agttcgttcg cggtgctcgt gtcgatgctg ctgtcgaaga tgaacggcag
      361 cgattcgacc gagctggtga ggttcgacgc cccggtcagc gtgccggcct gaaggacctt
      421 gtaggtcccc gcgacgtcgc cgagtgagac gaggttcacg tcgatcgtcg tgccggctcc
      481 gaagttcgcc gtgcccgtga catcgaacag ggtgccggta tcggtcgagg agtcgatcgt
      541 aacgccgagg gtcgatccgt cgcccgccga cagcgacgcg aggctcacag ttccggtgtt
      601 cgtagccgtg aggctcgtcc cggcgccgag ggtcaccgcc aggccggcgg tcccggtcag
      661 ctgtccggta aaggctgcgg ttcccgccag cgtcagtacg tccgcgcctc cgccaaaatc
      721 gattccgccg aacagcttcg acgtgccgcc gagctggacg ctatccgaac cggcgccgaa
      781 ttggacggcg ccgttcatta tcgcgtcgcc cgacagcgcc agcgcgttgt ttccggcgcc
      841 aaatttggca gcaccggtga ccgtgccatc cgcgacgtcg agggtatcgt tacccgatcc
      901 gaagagaatc tggccgccga tctgcggacc cgcgccggag gtcaccgcaa gctggcgaat
      961 tgttgcgccg ccgccgtttg cgcgcaggtc gaaggcgatc gccttgtcgc cgagggtcga
     1021 ggcattggcc acgaaaatcg atccgctgtt ctcgatcagc gagacggtcc cggcattgtc
     1081 gacaatcgcc gtcgccgtgc cgtcgctccc ggcgcggccg gcgccgatgg tgccgctgtt
     1141 cttgatcgtg gtgacggtcg cgccggcgtc gatcgcgatc gcccgcgagg cggtggtcgc
     1201 cgtcccgcct ccggtcgcgg tgatcgatcc cgagttgacg atctggggca cgctcgcccc
     1261 cgcgccgatc cgcagggcgg tcgcattggc ctcgacagcc gctgtggcga tgactccggc
     1321 gttggtcatg ccgccggcaa tcgtcacggc attgccgagg ccgccgatga caacgccctg
     1381 gcccgccacg gtcttgtaga cgccgttgct cgtgatgctg cccttgagga ccagcccctg
     1441 gccgcccgac ccggccacgg cgccgatgtt cgttgcctgc gtggccgagc cgatgagcac
     1501 ggccggcgcg ccgctgagat tggttacggt ggcggtcgtc tcgtttgcgt cggggatgcc
     1561 gtcgtcatcc tcgtccgtat cggtcgtgct ggtgtcggcc ggcctggcgt cgaacaggat
     1621 gccgcctgcg acattgccgc cgatgatgat cgacgaaccg gcctgcagca gatcgtccga
     1681 gtcgagcttg gaggtatccg agggtggaga cgtgtagcgg tagccggtcg tctgcaccgt
     1741 tccctggaag acgaacttgc cgccgacgtc gccgttgatg gccacgccta cggcattggc
     1801 tccctgaacc tggatagtgc ccttcgacag gatcacgtcg ccgctgacgt cgcccgtctc
     1861 gatgccgacg ctatcgttgc cgaggatggt aatgtcattg ctgttcgaga gcgttccggt
     1921 gagcgggccc tcgaggacga tgccgcccga ctggttgcct tcgaccgcga tcttgccgct
     1981 gttggtgacc ggccccgtgt gagcgccgag gacatggatt ccgaagcgga cggacccttg
     2041 cgcgaagggc ccgtcgaggt cgccgtcact gtccgtatcg gttggcgtga acgcctcgtc
     2101 gatggtgatc gtgccggtgt tgaggatctc gccggtgagg cccgtattgg cgacgatgcc
     2161 ggcggaattg ttcgctccgg tgacctggat cgtgccttcg ttcttcacga aattgctggt
     2221 gttcatcgtc actgcggcgc cgctcgtcgg cttgaccgat ccggtgctgc tgattcggat
     2281 gtcgccggac gcgctggtcg tctgcggcgt ggtcaccgcc gtggagatga ccgtttcggc
     2341 ctgggcgggg aacgccatcg ttgcagccgc aattgcacca aaacccgtcg tcgcaagcag
     2401 gaatcgcatt aagagcccct tcgtctcgcc gcttcagcga tgcggctacc taggttggac
     2461 gcagacctcc ccggacgtgt tcgcgcccgg gcaaatgcct gccgagattg aagccggatt
     2521 cggctgtcgc cgcgcccgtt acccaccccg gaatgacggg ggcctctagg ctgctcgcga
     2581 cattaagcca agatgaatac tgaccaagcc gctgcgttgg cgcgacaatc ctccggcgct
     2641 ttcccccggg cgcgtggcgg gatagacaca ggttaatgga cccgattctt caaaccatcg
     2701 ctcccgtcac tcacgacctt ggcgggttca aggtccaccg caccttgccg ggcaaggagc
     2761 gaaccatggt cggcccgttc atcttcttcg atcagatggg ccccgctcgg ctcggtcccg
     2821 gcgagggcat cgacgttcgg ccgcatccgc acatcaacct tgctacggtg acctttctgt
     2881 tcgccggggc gatcgaccat cgcgacagcc tcggaacggt gcagcgaatc gagcccggcg
     2941 cggtgaacct gatgtcggcc gggcgcggaa tcagccactc ggagcgctct ccggcggacg
     3001 agcgctccgc gggagcgcag ctcgacggga tccagacctg gctcgctctt ccgcgcgaca
     3061 aggaggagat gcttcccgac tttgagcatg tcgcggccga tcgtctgccc gagctggatt
     3121 ccgacggcgt gcggctgaag ctcgtgatgg gcgattggga gggcgcgtcc tcgccggtga
     3181 cgcagcattc gccgaccatc tatgcggcaa tccatctcgc cccgggagcg gcgctggtca
     3241 tcaaccatga ggccgacgaa agggcgctct acctcctcga aggggatgcc acggtcgacg
     3301 gcgtgccgct cctggcccag aaccttgcgc tcctcgctcc ggggcacctg ccgctgctgt
     3361 cgtcatccgg cggcgcccgc ctgatgctgt gcggcggcgc tccgatggac ggcgagcgac
     3421 acgtctggtg gaacttcgtc tcgtcgagcc gcgaccggat caacgaggcg aagcgcgcct
     3481 ggcgctcggg cgagttcgcg cttccgccgg ccgatcacga cgagttcatc ccgctgcccg
     3541 aggtgccgct gacggtcagc tatccgtgac ggagttccgc cgagtcggac tgtcgaccgg
     3601 catcaccctc aacgtcgcgt tcgccggcgc agaggatgcc ccggctgtca tcctgctcca
     3661 cggcttcccg gaatcgcacc gcacctggcg cgaggtcgcg ccgcggctgg aagacagttt
     3721 ccggctgatc atgcccgacc agcggggctt tgccggatcg gaccggccgc aggaggtgga
     3781 ggcgtacaag accgatacct tgatcaacga catcttcgcg cttgcggaca cgctgggact
     3841 ggagagcttc gcgctcgtcg ggcatgactg gggcggagcg atcgcctggg tggctgcgct
     3901 tcagcacccc tatcgggtga agaggctggt gatcgtcaat gcaccgcatc
//
LOCUS       DASSGX010000100         3418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_563612, whole genome shotgun sequence.
ACCESSION   DASSGX010000100 DASSGX010000000
VERSION     DASSGX010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563612"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2434)
                     /locus_tag="VFI12_01780"
     CDS             complement(<1..2434)
                     /locus_tag="VFI12_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET7055158.1"
                     /translation="MALAEPVSRFELAPRRENFSHIPEVLPLPNLIQTQTESFEWFVK
                     TGLRELLDEITPITDFTGKNLELHFRDFYFEDPKFSEEECRTRDLTYSRPLKVEVDLI
                     IKETDEVKHQTVFMGDFPWMTDQGTFIINGAERVVVSQLVRSPGVYYTASEDPATGRH
                     LFAAKVIPNRGAWLEFETSNKDLLSVKVDRKRKIPVTTLLRAVGFEDNQEMLSLFAPV
                     DDNPEHPFVALTLDKDTAATQEEALIEVYKKLRPGDPPTIDNARALVTSLFFNFRRYD
                     LGRVGRYKLNKALGEAAAKLKIELPAEESANPRDSRVISKNDIVAIIGKLVELNNGRG
                     EPDDIDHLGNRRIRANGELIQNQFRIGLLRMERVVKERMTITDAEQATPNALINIRPV
                     VAAMKEFFGGSQLSQFMDQTNPLAEITNKRRLSALGPGGLSRERAGFDVRDVHPSHYG
                     RMCPIETPEGPNIGLIGSLATYGKINRHGFIETPYRIVRKAVSYKDKQADAIGETLLS
                     DVVPADGAKPLAKAGSVIDDKTWAAIKEHKLQGVRIRPRVTDEIQYLDASEEEKFSIA
                     QANAALDESQHLTEDRVMVRYRDRFLTVPVEEVDYMDVSPKQIVSVATAMIPFLEHDD
                     ANRALMGSNMMRQSVPLLQPEAPLVGTGVEYRAAFDSQQVVVAKAGGTVVSVTANHIQ
                     VERDEGDVRDTYALKKFLRSNQGTCINQRPIVSVGDRVAENQVLADSSSTDHGEMALG
                     QNILVAFLPWEGGNYEDAIVLSERMVKDDLFTSIHIEKHEIEARDTKLGPEEITRDIP
                     NVGEESLRNLDED"
     gene            complement(2541..>3418)
                     /locus_tag="VFI12_01785"
     CDS             complement(2541..>3418)
                     /locus_tag="VFI12_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055159.1"
                     /translation="THAASDAPRWSSSRRTVSLIASVRNRAIKAAAYSRQFYPDLIAE
                     LRADDSRLSPRMNEITGLVLKRAANDLADIIYSAAHGKGVSPSVASLKATVRYHFPRK
                     NEPHQQVFVRARDSAGNPIEGLRVDVDWPLADGGTKHLSIYTDEHGRSHWTDGIGASP
                     LMVKRVFRATATTNRHTETDTTWFKTSPVPADGQAGFLTTINDHSPSVGSRAVVKSRL
                     RDTSGRPIVGIPVTWEWTFPNRVVTKTDETNSNGVARSSYRIRSTTTRQFVTVYAYAQ
                     SGSKNRRSSVGFQRS"
BASE COUNT          502 a         1073 c         1178 g          665 t
ORIGIN      
        1 cgtcctcgtc gaggttgcgc aggctctcct cgcccacgtt cgggatgtcg cgcgtgatct
       61 cctcggggcc gagcttggtg tcgcgggcct cgatctcgtg cttctcgatg tggatgctgg
      121 tgaacaggtc gtccttgacc atccgctcgc tgaggacgat ggcgtcctcg tagttgccgc
      181 cctcccacgg gaggaaggcg accaggatgt tctgccccag cgccatctcg ccgtggtcgg
      241 tgctgctcga atcggcaagc acctggttct cggcgacgcg gtcgccgacg ctgacgatgg
      301 gccgctggtt gatgcaggtc ccctggttcg agcgcaggaa cttcttgagc gcgtacgtgt
      361 cgcgcacgtc gccctcgtcc cgctcgacct ggatgtggtt ggccgtgacg ctcaccacgg
      421 tgccgccggc cttcgccacc accacctgct gcgagtcgaa tgcggcgcgg tactcgacgc
      481 cggtgccgac cagcggcgcc tccggctgga gcagcggcac cgactggcgc atcatgttcg
      541 agcccatcag cgcgcggttc gcgtcgtcat gctcgaggaa cgggatcatg gcggtcgcca
      601 cgctgacgat ctgcttgggg ctgacatcca tgtagtcgac ttcctcgacc gggaccgtca
      661 ggaatcggtc gcggtagcgg accatgaccc ggtcctcggt caggtgctgg ctctcgtcga
      721 gcgcggcgtt cgcctgcgcg atcgagaact tctcctcttc cgaggcatcg aggtactgga
      781 tctcatccgt cactcgcggc cggatgcgga caccctggag cttgtgctcc ttgatcgcgg
      841 cccaggtctt gtcgtcgatg accgatccgg ccttggccag cggcttggcg ccgtcggccg
      901 gcacgacgtc actgagcagc gtctcgccga tcgcatcggc ctgcttgtcc ttataggaga
      961 ccgccttgcg cacgatgcgg tacggcgtct cgatgaaccc gtgacggttg atcttgccgt
     1021 aggtcgccaa cgacccgatc agaccgatgt tcggcccttc gggcgtctcg atggggcaca
     1081 tgcggccgta gtgcgacggg tgaacgtcgc ggacatcgaa gccggcccgc tcgcgggaca
     1141 ggccgccagg ccccagcgcc gacaggcggc gcttgttggt gatctcggcc agcgggttgg
     1201 tctgatccat gaactgggac agctggctgc cgccgaagaa ttccttcatg gctgccacca
     1261 ccgggcggat gttgatgagc gcgttgggcg tcgcctgctc ggcatcggtg atcgtcatgc
     1321 gctccttgac gacgcgctcc atgcgcagca ggccgatccg gaactggttc tggatcagct
     1381 cgccgttggc ccggatgcgc cggttgccga gatgatcgat gtcatccggc tcgccgcgcc
     1441 cgttgttgag ctcgaccagc ttgccgatga tggccacgat gtcgttcttg ctgatgaccc
     1501 gcgagtcgcg cggattggcg ctctcctcgg cgggcagctc gatcttcagc ttggccgcgg
     1561 cctcgcccag cgccttgttc agcttgtagc gcccgacgcg gccgaggtcg tagcggcgga
     1621 agttgaagaa gaggctggtg accagcgcgc gcgcgttgtc gatggtcggc ggatcgccag
     1681 ggcgcagctt cttgtagacc tcgatcaacg cctcttcctg ggtcgcggcg gtgtccttgt
     1741 caagggtgag cgcgacgaag ggatgctcgg ggttgtcgtc caccggggcg aacaggctga
     1801 gcatctcctg gttgtcctcg aagccgacgg cgcgcaggag cgtggtgacc gggatcttgc
     1861 gcttgcggtc gaccttgacc gacagcaggt ccttgttgct ggtctcgaac tcgagccacg
     1921 caccgcggtt cgggatgacc ttggcagcga acaggtgccg gccggtggcc ggatcctcgg
     1981 aggcggtgta gtagaccccc ggcgagcgca ccagctggct caccaccacg cgctcggcgc
     2041 cgttgatgat gaaggtgccc tggtccgtca tccacgggaa gtcgcccatg aagacggtct
     2101 ggtgcttgac ctcgtcggtc tccttgatga tgaggtcgac ctcgaccttg agcggccggc
     2161 tgtaggtcag gtcacgggtt cggcactcct cttcgctgaa cttcgggtcc tcgaagtaga
     2221 agtctcggaa gtgaagctcg aggttcttgc cggtgaagtc ggtgatcggg gtaatctcgt
     2281 caagcagctc gcggaggccg gtcttgacga accattcgaa ggattcggtc tgggtctgga
     2341 tcagattcgg gaggggaagg acctccggaa tgtgcgagaa attctctcgg cgcggggcca
     2401 gctcaaaacg gctgacgggc tcggccaaag ccattcagga ctcctaaaag agggcagggt
     2461 gaggcgcgga cacgcgaacg aggagtatac caaaccaatg cgcttcgtca actgaagcgc
     2521 ccccgcgact gccctctcgt tcagctgcgc tggaatccga ccgacgagcg ccggttcttg
     2581 ctccccgact gggcgtaggc gtagaccgtc acgaactgcc tggtcgtcgt cgatcggatt
     2641 cgataggacg agcgcgcgac gccgttcgag ttcgtctcgt cggtcttggt cacgacgcgg
     2701 ttcgggaagg tccactccca tgtcaccgga atcccgacga tgggccggcc cgatgtgtcg
     2761 cgcaggcgtg acttcacgac ggcgcgactc ccgaccgacg gtgagtgatc gttgatcgtg
     2821 gtcaggaagc cggcctgccc atccgccggc accggcgacg tcttgaacca ggtcgtatcg
     2881 gtctcggtgt ggcggttggt ggtggcggtc gcccggaaga cgcgcttcac catcagcggg
     2941 ctggcgccga tgccgtccgt ccaatggctt ctgccgtgct cgtcggtgta gatgctgagg
     3001 tgcttcgtgc cgccgtcggc cagcggccag tccacgtcga cgcgcaggcc ttcgatggga
     3061 ttgccggcgc tgtccctggc tctgacgaag acctgctggt gcggctcgtt cttgcgcggg
     3121 aagtggtagc gcaccgtggc cttcagcgaa gcgacggacg gcgagacccc cttgccgtgc
     3181 gccgccgaat agatgatgtc ggccaggtcg ttggcagccc gcttgagcac caggccggtg
     3241 atctcgttca tccgcggcga gagccgggag tcgtccgcgc gcagctcggc gatgaggtcg
     3301 ggatagaact ggcgagagta ggcggccgcc ttgatggccc ggttgcggac gctggcgatg
     3361 aggctgacgg tgcgccggct gctcgaccac cgcggagcgt cgctcgcggc gtgggtct
//
LOCUS       DASSGX010000101         2512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_128106, whole genome shotgun sequence.
ACCESSION   DASSGX010000101 DASSGX010000000
VERSION     DASSGX010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128106"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..1609
                     /locus_tag="VFI12_01790"
     CDS             176..1609
                     /locus_tag="VFI12_01790"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7055160.1"
                     /translation="MTDSNRQIGDLFKEFRRGEVNRREFLTRGTALGISAVFLGRMVT
                     DKAFAQDATPAAAAPGWSLTTPEGFDQSLAGQKITVVLGSDGPGTPFEKACVKLFTDT
                     TGVEVNYIAGAQSTTDRLANYLQILNAQASDIDAMMIDVIHPGILAVHALDLTDAMKA
                     NNVDYFQAIVDNNTVDGKLVGIPYYTDAGLLYYRKDLLDKYSISGPPATWADLETASK
                     TIMDGERGGGASEFWGFVWQGNAYEGLTCDGLEWQVSNGGGNIIEPDGTISIDNPQAI
                     AAFERAKGWVGTISPEGVTTFQEEEARNIWQGGNSAFMRNWPYAYAPGQTADSVIKDK
                     FAVTLLPKGDGENARNAATLGGWQMMASKYSKSPDAATLFCRFMTSTVIERSSAIERA
                     LLPTIASVYDDPDVLKANPYYADLKPVFQGGAVPRPSTVSSTSYNDVSTSYFTHLHNI
                     LTGSGDAASEVKAMADELKTIMDELNS"
     gene            1711..>2512
                     /locus_tag="VFI12_01795"
     CDS             1711..>2512
                     /locus_tag="VFI12_01795"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET7055161.1"
                     /translation="MASERVGITASAAAPAGGRLRETAPVSEWAKARERLAWLFVAPA
                     ILVVAIVAIYPLIKTFQLSLTNARLGLGIKTRYVGLDNYRYLYHDDKFLSSLKHTAYF
                     TVASVGFETLLGMIIALTIHSNFRGRGLVRTSMLVPWAIPTVVSAQMWKWMYIDVYGV
                     IPDFFGNKLHIMEPRTALLANTSTSLWAIVAVDVWKTTPFMALLLLAGLQVIPGDIYE
                     SANIDGASKFRQFFDLTLPLLKPALLVALIFRTMDAFRVYDVVAVMKGQ"
BASE COUNT          481 a          827 c          750 g          454 t
ORIGIN      
        1 ggacgtcggt gtgtaggcac atatccggtt gcggcttgac ctcgctcgaa tgtgcgtggt
       61 actacaagaa ctccggcaac tgtctcgcca gcccccttcc agaacccggg agggtgagcg
      121 ggtgccaaac ggcggcgatg tgcgccgcgc acgactcagt gaatggagtt gagcagtgac
      181 cgacagcaac cgtcagatcg gcgatctctt caaggagttc cgccgcggcg aagtcaaccg
      241 ccgcgaattc ctgacccgtg ggaccgccct cggcatcagc gcggtcttcc ttggccggat
      301 ggtgaccgac aaggccttcg ctcaggacgc aacgccggca gccgcggcgc ccggctggtc
      361 cctgacaacg ccggaaggat tcgatcagag cctcgcgggc cagaagatca cggtcgtgct
      421 gggctcggac ggccccggca ccccgttcga gaaggcttgc gtcaagctct tcacggacac
      481 caccggcgtc gaagtcaact acatcgccgg cgcgcagtcg accaccgatc gcctcgccaa
      541 ctacctgcaa atcctcaacg cgcaggccag cgacatcgac gcgatgatga tcgacgtcat
      601 ccatcccggc atcctcgccg tccacgcgct cgacctgacc gacgccatga aggccaacaa
      661 cgttgactac ttccaggcga tcgtcgacaa caacacggtc gatggcaagc tggtcggcat
      721 cccctattac accgacgccg gtctgctcta ctaccgcaag gacctgctcg ataagtacag
      781 catctccggc ccgccggcga cctgggctga tctcgagacg gcctccaaga ccatcatgga
      841 tggcgagcgc ggcggcggcg cctccgagtt ctggggcttc gtctggcagg gcaacgccta
      901 tgagggcctg acctgtgacg gcctcgagtg gcaggtgtcc aacggcggcg gcaacatcat
      961 cgagccggac ggcacgatct cgatcgacaa cccgcaggcg atcgccgcgt tcgagcgcgc
     1021 caagggctgg gtcggtacga tctctccaga aggcgtgacc acgttccagg aagaagaggc
     1081 gcgcaacatc tggcagggcg gcaactctgc cttcatgcgc aactggccgt atgcctatgc
     1141 acctggtcag acagccgact cggtcatcaa ggacaagttc gcggttacgc tgctgcccaa
     1201 gggcgacggc gagaatgccc gcaacgcggc caccctcggc ggttggcaga tgatggcgtc
     1261 caagtattcg aagagcccgg acgccgccac gctgttctgc cgcttcatga cgtccacggt
     1321 catcgagcgg tcgtccgcga tcgagcgcgc gttgctgccg acgatcgcct cggtctacga
     1381 cgatccggac gtgctcaagg cgaacccgta ctacgccgac ctgaagcccg tcttccaggg
     1441 tggcgcggtg ccgcggccgt cgaccgtctc cagcacctcg tacaatgacg tctcgacctc
     1501 gtacttcacc catcttcaca acatcctgac cggatccggc gatgccgcgt ccgaggtcaa
     1561 ggcgatggcg gacgagctca agacgatcat ggacgagttg aactcctaaa cgctccggct
     1621 ccttgccggg tgtgacaggg tgatagcagg ccgggcgggg gcacggagcc tccgcccgct
     1681 cctgtacctc caatggggga ggtttgggcc atggcgagcg aacgagttgg tatcactgct
     1741 tccgcggccg cgccggccgg cggacggctc cgggaaacag ctccggtcag cgaatgggca
     1801 aaggccagag aacgtctcgc gtggctcttc gtggctccgg caatcctggt cgttgccatt
     1861 gtcgcgatct atccactgat caagaccttt cagctttcgc tgacaaacgc ccggctcgga
     1921 ctcggtatca agaccagata cgtcgggctc gacaactatc gatacctcta tcacgacgac
     1981 aaattcttga gctccctcaa gcacacggcc tatttcacgg tcgcgtcagt gggattcgag
     2041 acactgctcg gcatgatcat cgcgctgacc attcattcga acttccgcgg acggggcctc
     2101 gtccgcacat ccatgctcgt gccgtgggcg atcccaaccg tcgtctcggc gcaaatgtgg
     2161 aaatggatgt acatcgacgt ttacggtgtc atccccgact tcttcggcaa caagctgcat
     2221 atcatggagc ccaggaccgc gctgctcgcc aacacatcga catcgctttg ggcaatcgtc
     2281 gccgttgatg tctggaaaac gacgccgttc atggcgctcc tgctcctggc cggcttgcag
     2341 gtcattccgg gcgacattta tgaatcggcg aacatcgatg gcgcgagcaa gttccgccaa
     2401 ttctttgacc tgacgctgcc acttctgaag ccggcattgc tggtcgcgct gatcttccgg
     2461 acgatggacg cgttccgcgt ctacgacgtc gtcgccgtca tgaagggcca gt
//
LOCUS       DASSGX010000102         4278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_890616, whole genome shotgun sequence.
ACCESSION   DASSGX010000102 DASSGX010000000
VERSION     DASSGX010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4278
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890616"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VFI12_01800"
     CDS             complement(<1..445)
                     /locus_tag="VFI12_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055162.1"
                     /translation="MIDLARTYRRFLVVAAVLIFFTNIADFTVKYGLIPLVWIGIFAA
                     LSAPTILLGLGGARIPIRPVVWWCAFYLLISIASYYHSPQDEFAFQEVQTRIVSVIFL
                     VLAMIVFSGAEEQRLAQKWIAGAVLLATALNIYELFNPMTYSTIPG"
     gene            complement(442..1611)
                     /locus_tag="VFI12_01805"
     CDS             complement(442..1611)
                     /locus_tag="VFI12_01805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7055163.1"
                     /translation="MKILFLHRFKDVAGVEREFGGAERQLVDLVCGLQRRGHAVTLVT
                     FYPGGGMLADAERAGVRIVSLDKTGRWDIPGFLFRLVRVLRRERPEIVHGYLGLANSL
                     LALTRPIHRASVVWGVRASDIDLSRYHWVARVDAWLEQKLSRFPDLIIANSHAGKQHA
                     IGLGYPAGKMTVIHNGIDLDRFQRDEPGRERVRAEWGVRPGELLIGRIGRIDPQKDQP
                     TFLEAAAKVASVRPDARFVIVGNDQFGEQDEITPLIERLNLGDRLIWAGHRADMAAVH
                     SAFDLCVSSSAFGEGTPNVLVEAMACGTPCVTTDVGDSAIAVGTFGSVVPRRDPAALA
                     KAILAALDAPVDRAALRAHVAADFSLDHLITATETAIAGLRDAAGAGHRAPSMQP"
     gene            complement(1614..2714)
                     /locus_tag="VFI12_01810"
     CDS             complement(1614..2714)
                     /locus_tag="VFI12_01810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET7055164.1"
                     /translation="MAVGRITFVIPTLTSGGAERVLTTMANYWATQGKSVTVLTMSPQ
                     NDPPFYPLLPTVPRIGLGITRAARTPAHAVVNNFGRLRRLRRAIRASRPDAVISFLDQ
                     SNVLTLLATRRTGLPVIVSEHTDPGLARRNPAWDRLRSMTYPRASKIVVLTEASKAFF
                     PSAIQARTCLIPNPVLIDPPSESIARTGLPHIAAMGRFGPEKGFDRLIDAFALVADQF
                     PEWDLLIWGDGDLRPELAAQIDRLDLTQRILMPGRTTAPHAEMRRAEIFAMTSHREGF
                     PMALAEAMGCGLPAVAFDLPSGPRAIIRDGIDGVLVPNGDIPAFAEALASLMRSPECR
                     ATMAARAPEVLDRFGVERVMTIWDAMLEELTA"
     gene            complement(2714..4087)
                     /locus_tag="VFI12_01815"
     CDS             complement(2714..4087)
                     /locus_tag="VFI12_01815"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HET7055165.1"
                     /translation="MAVESRALPFSGPLGRLGSHRQLLLNIAIVAFCSVGVKAAGMIR
                     DVVLASEFGTSDAADAFIAAWAIPQFLAIIAGNAFAGVIIPLQAEARGRGGEAQSRRF
                     LSEMLLLSIAGLAAITLLLVPFRDQLLPLVTANFGPAKLAETRELWMIMLPAVFLFAM
                     ATIWSAMLNTDDRFGLAALSPMLVPFSTIAALWIHPQGGITSPTVGFVIGSLLQVLWL
                     LFGLRRNNLTILPVWHGGVAETRMALRQFVPYLANGVVFGGVGVVDQAMAATLGQGSL
                     ATLSYGNKLVLPVLAIGSAALATVVYPRFSRLVAEREWTRLQHQVKAYLTLTLLATVP
                     ATALIVFGSTVIVQLLFEHGEFNPADTAAVARLQTIFALMIPAYTLSQLLSRVLNAMR
                     ATRYLLIGSIVIFAFNITADYLFKEWFGITGIALATVGNYLLALAFNLYLFRRLITAR
                     LSAQGSP"
BASE COUNT          780 a         1356 c         1518 g          624 t
ORIGIN      
        1 ggccggggat cgtgctgtag gtcatcggat tgaagagctc gtagatgttg agcgcggtgg
       61 ccagcaggac cgcgccggcg atccactttt gggccagccg ttgctcctcg gcgccgctga
      121 agacgatcat cgccagcacc aggaagatga ccgaaacgat ccgagtctgc acctcctgga
      181 aggcgaactc gtcctgcggc gagtggtagt acgacgcgat gctgatcagg aggtagaagg
      241 cgcaccacca gacgacaggc cggatgggga tccgcgcgcc gcccaggccg agcaggatcg
      301 taggtgcgct cagggccgcg aagatgccga tccaaaccag cggaatcagg ccgtacttga
      361 ccgtgaagtc ggcgatgttc gtgaagaaaa tcaggaccgc ggcgaccacc aggaaacggc
      421 ggtaggtccg ggcgagatcg atcatggctg catcgacggc gcgcgatggc cggccccggc
      481 cgcgtcccgt aacccggcga tggcagtttc ggtcgcggtg atcaggtgat cgagcgagaa
      541 gtcggcggca acgtgcgcgc gcagggcggc ccgatccact ggagcgtcga gcgcggccag
      601 gatggccttg gccagggcgg cgggatcacg ccggggcacg accgacccga acgttccgac
      661 cgcaatcgcc gaatcaccga cgtcagtcgt cacgcagggc gtgccgcagg ccatcgcctc
      721 gaccagcacg ttcggcgttc cctccccgaa ggccgaggag gagacgcaga ggtcgaatgc
      781 gctgtggaca gccgccatgt cggcccggtg cccggcccag atcaggcgat cgcccaggtt
      841 cagccgttcg atcagcggcg tgatctcgtc ctgctccccg aactggtcgt tgccgacgat
      901 cacgaaccgg gcatcaggac ggaccgaagc aacctttgcc gcggcttcca ggaaggtcgg
      961 ctggtccttt tgcggatcga tccgaccgat tcgaccgatc agcagctccc ccggccgcac
     1021 gccccactcg gcgcggaccc gctcccggcc aggctcatcg cgctggaagc ggtcgaggtc
     1081 gatgccgttg tggatgaccg tcatcttccc ggccgggtag ccgaggccga tcgcgtgctg
     1141 tttgcccgcg tggctgttgg cgatgatcag gtccgggaat cggctgagct tctgttcgag
     1201 ccaggcatcg acccgcgcca cccagtggta gcggctgagg tcgatgtcgg acgcccggac
     1261 gccccagaca acgctcgcgc ggtggattgg ccgggtcagg gcgagcagcg agtttgcgag
     1321 accgaggtat ccgtggacga tctcgggccg ttctcgccgg aggacacgga ccaggcgaaa
     1381 caggaagccg gggatgtccc agcggccggt cttgtcgagc gagacgatcc ggacgccggc
     1441 gcgctccgcg tcggccagca tcccgccgcc cgggtagaag gtaacgagcg tcaccgcatg
     1501 gccccggcgt tgcaggccgc agacaaggtc gaccaattgc cgctcggcgc cgccgaactc
     1561 gcgctcgacc ccggcgacgt ccttgaaccg gtgcaggaag aggatcttca tgattacgcc
     1621 gtcagttctt ccagcattgc atcccaaatc gtcatcaccc gctcgacacc gaagcgatcg
     1681 agcacctcgg gggcccgggc ggccatcgtc gcccggcatt cgggcgaccg catcagcgat
     1741 gctagagcct cggcgaaggc gggaatatcc ccatttggaa caagaacgcc atcgatgccg
     1801 tcccggatga tcgcgcgggg tccgctcggc aggtcgaagg cgacggccgg cagcccacac
     1861 cccatcgcct cggccagcgc catcgggaag ccctcgcggt gcgaggtcat ggcgaagatc
     1921 tcggcgcggc gcatttccgc atgtggcgcg gtcgtccggc cgggcatgag gatgcgctgg
     1981 gtcaggtcga ggcggtcgat ttgggctgcc agctctggtc gcagatcgcc gtcgccccag
     2041 atcaggagat cccactccgg aaactggtcg gcgaccaggg cgaaggcgtc gatcaggcgg
     2101 tcgaacccct tctcgggccc gaaccggccc atcgcggcga tgtgcgggag gccggtcctg
     2161 gcaatcgact cgctcggagg atcgatcagg accgggttcg ggatgaggca ggttctggcc
     2221 tggatcgccg acgggaagaa cgccttgctg gcctcggtca gcaccacgat cttgctcgcg
     2281 cggggatagg tcatgctccg gaggcggtcc caggccggat tgcggcgcgc gaggccggga
     2341 tcggtgtgct cgctgacgat aacgggcagt ccggtcctgc gggtcgcgag cagggtcaga
     2401 acattggact ggtcgaggaa gctgatgacc gcgtcgggcc ggctggcccg gatcgcgcgc
     2461 cgcaagcgtc tgaggcggcc gaagttattg accaccgcgt gggctggtgt cctggccgcg
     2521 cgggtgatgc ccagaccgat gcggggtacg gtgggaagga gcgggtaaaa gggcgggtcg
     2581 ttctgcggcg acatcgtcag gacagtgacg gacttgccct gcgtggccca gtagttggcc
     2641 atggtggtca ggacccgctc cgcgccgccg ctggtcaggg tcgggatcac gaaagtgatg
     2701 cgcccaaccg ccatcacgga ctgccctggg cagacagccg ggccgtgatc agccggcgga
     2761 acaggtacag gttgaaggcg agcgcgagca ggtagttgcc gaccgttgcg agggcgatgc
     2821 cggtgatccc gaaccattcc ttgaagaggt aatcggcggt gatgttgaag gcgaagatga
     2881 cgatcgagcc gatcagcaga taccgcgtcg cccgcatcgc attcagcacg cgcgacagca
     2941 actgcgagag ggtgtaggcc gggatcatca gggcaaagat cgtctgcagc cgggccacgg
     3001 cggcggtgtc cgccgggttg aactcgccgt gctcgaacag gagctggacg atcacggtcg
     3061 agccgaacac aatcagggcc gtagcgggca ccgttgccag cagcgtcagg gtgaggtagg
     3121 ccttgacctg gtgctggagg cgcgtccact cgcgctcggc caccagccgg gaaaatcggg
     3181 gatagaccac ggtggccagc gcggccgagc cgatcgcgag gaccggcagc accagcttgt
     3241 tcccgtagct cagggttgcc aggctgccct ggccgagcgt cgcggccatc gcctgatcaa
     3301 ccacaccgac cccgccgaag accaccccat tggcgaggta gggcacgaac tggcgcagcg
     3361 ccattcgcgt ctcggccaca ccgccatgcc agaccggcag gatggtgagg ttgttgcgac
     3421 gcaggccgaa cagcaaccac agcacctgga gcagggaacc gatgacgaac ccgactgtcg
     3481 gcgaggtgat cccgccctgc gggtggatcc agagcgcggc gatcgtggag aaggggacca
     3541 gcatcgggga gagggcggcg agaccgaagc ggtcatccgt gttgagcatg gcggaccaga
     3601 tcgtcgccat cgcgaagaga aagacggccg gcaacatgat catccagagc tcgcgggttt
     3661 cggccagctt ggcggggccg aagttggcgg tcacgagcgg caggagctga tcccggaacg
     3721 gaacgagcag gagcgtgatg gcggcgagcc cggcgatgct gagcagcagc atctcgctca
     3781 ggaagcggcg ggactgcgcc tcgccgcctc gtccccgcgc ttccgcctgc agcgggataa
     3841 tcacgccggc gaaggcgttg ccggcgatga tcgcgaggaa ctgcggaatt gcccaggccg
     3901 cgatgaaggc gtcggccgcg tcggacgtac cgaactcgga ggccagcacg acatcgcgaa
     3961 tcatcccggc ggccttgacg ccgacggagc agaacgcgac gatcgcgata ttgaggagga
     4021 gctgccggtg gcttcccagt cgcccgagcg gtccggagaa gggcagcgcg cgactttcga
     4081 cagccatggg cgggatcact caacgcgcat cacggtccat ctggtccgtg cgtcagtctc
     4141 cagcatgccc caaacgggcc atgcccagac accgcgcggc agccccggtc gtcccaattg
     4201 caacgtgacg aatgtcccga tgcacgatcg gacgctatcc gccggagacg acctcgatcg
     4261 tgccggtgtg atccggga
//
LOCUS       DASSGX010000103         7211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_999019, whole genome shotgun sequence.
ACCESSION   DASSGX010000103 DASSGX010000000
VERSION     DASSGX010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7211
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_999019"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /gene="accD"
                     /locus_tag="VFI12_01820"
     CDS             <1..869
                     /gene="accD"
                     /locus_tag="VFI12_01820"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HET7055166.1"
                     /translation="KCEELIYQPELEHNMRMCPKCGYLHRMSARQRIELLADPDSFQE
                     WDTELRTANPLGFSEPSGSYDSKIARTMARSGEQESLITGRMTVSGYGVAITVADFSF
                     LGASMGSVFGEKLARAAERALDERLPVLTVNASGGARMHEGLFSLMQMAKTIAALRLL
                     GGARLPHISLLTDPCYGGVSASYAMVADVILAEPGALVGFAGPRVIEQITKQKLPDGF
                     QTAEFLLEHGMIDGIVARPDLPTELAQLLAHYSRIRPSDPILVTSDEPGRTVESPVPA
                     IELAHLNGGVRA"
     gene            866..1759
                     /locus_tag="VFI12_01825"
     CDS             866..1759
                     /locus_tag="VFI12_01825"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HET7055167.1"
                     /translation="MSSVVNRRTRRPGKPSEPEIVEPGNPGGLTAWDRVKLARHPDRP
                     HALDYISELTTDFVELHGDRSFGDDHALIGGLANFAGRTVMVLGHQKGRDTKENIQRN
                     FGMARPEGYRKAERLMRHAEKFGMPVITFIDTPGAEPGIGSEERGQSTAIAESLLTMA
                     GLRTMVVAVVIGEGGSGGALAIGVADRVLMLENAIYSVASPEASATILWKDVAKASDA
                     AASMKITALDLYGFGMIDEIIPEAHPAHERPREVIAAAGSAIRRHIGDLHAQIQQENG
                     PDELVQHRYEKFRRIGSFTES"
     gene            1827..2501
                     /locus_tag="VFI12_01830"
     CDS             1827..2501
                     /locus_tag="VFI12_01830"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET7055168.1"
                     /translation="MTYVALEIGRKRVIASALEWPGWARVGRDEYAALETLRVYGPRY
                     QRAIASVASDFRIPERVEEFVVAERLTGDATTDYGVPVIAPSADAAPLDDAAMYRLRA
                     ILGACWDALERAAASVEGKELRTGPRGGGRSTPQILDHVIDAHFGYLKRLAWREPHER
                     IDDLSTEFAAITEINASALAFAMSGEMPATGPRGGKLWTPRYFIRRAAWHILDHAWEI
                     EDRLDA"
     gene            complement(3100..3873)
                     /locus_tag="VFI12_01835"
     CDS             complement(3100..3873)
                     /locus_tag="VFI12_01835"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7055169.1"
                     /translation="MLHLRSVRIRNPEALPEGYPFDLPIILALDELELTAPVAFLVGE
                     NGSGKSTLLEGIAASANLPTIGSVELERDETLSAAQELGRHLVLSWSSRNHRGFYMRS
                     EDFFGFGLSTQRTIAELDQIASTLEPGTYAHAAVMAQRNALDRKYGDLNARSHGEGFF
                     QVFAERFTPGGLYLLDEPEVALSPQRQIALLALMKEVVGQDAQFIIATHAPMLMAYPG
                     AQILYLSEHGIREQTWDDTEHVQMTRTFLMDPDLFLRHL"
     gene            4040..4552
                     /locus_tag="VFI12_01840"
     CDS             4040..4552
                     /locus_tag="VFI12_01840"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HET7055170.1"
                     /translation="MTVVPELLAANECYAAQFTYGDLPVPPAKRLVVLTCMDARIDPL
                     RALGLKEGDAHVLRNSGGRPTPDMLRSLAVSQEMLGTREIVVIHHTECGSVRITNELM
                     RERLAENIGRDAAIAANVIDFLPFRDLAQSVRDDLAVLRATPLLRPETEVSGFIFDVK
                     TGRIHAVDSE"
     gene            complement(4622..>7211)
                     /locus_tag="VFI12_01845"
     CDS             complement(4622..>7211)
                     /locus_tag="VFI12_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055171.1"
                     /translation="IITSQRLAFARGGEVVLDIVRWWLKRPPYLSGNGAVNLRIETNS
                     GHRDRLQFTTRDSRQAATELVTLLTHGKQALPSVPLDPIFDEPPVRRSERRRVTAYQP
                     DLTREPEPAPEAFQRSDEHKYTADVIDATTLQVLDSTDFPPVLETQTPSTHHGSVVEG
                     GRVSGDPITISTLANQTHRGGDWSLIPIPALMPRSSKGNKAGWAFRLSGLIALIAFAA
                     AFGGNRLPDTSRLVDPIIAPQTQQTPGGSVAQVIPTSTATVPVIDGVNAPTREAAETA
                     IAIGVGGETAPAASATPSVPAATATNTASPTATATPKPRPTVTKTAPTWPTLAAAEAT
                     DEPTLAPTETPTLEPTATDTPEPTATATDTPEPAATATDTPESTATDTATAEPTAADT
                     PTPEPTATDTPTPEPTATETPTVEPTATDTPSPEPTATDTPTPEPTNTPEPSPTATAT
                     EEPVFGPPDPTSDHPTETPTVEPTATNTEVPTETPTTVPTDTPTATATETAVPTATAT
                     ATTPPTATNTPEPTATETPEPTPKAGFPAQERSVRDGEEPDQVFSTGAFRYTVEFAER
                     GASLPTLDLTEVPGQEWVTVVLYAQNWTEQPATLNMADFQLVVSGDFGWQFVGMDLSS
                     PDIARFLGFDPILETTELRSIKAGEGIRLALVYLIPPSTTNIELIDFTSGLNIGASLA
                     AGGDVTKLGKAPVRPDLIAATVTQVIDGRTIVVEADGTSATIQYLGVNVPTGNQCFTA
                     DSTQTNSNIVLGKTVYLEREFRNRVTADGDTYARDVWIDNGEGGLVLVAAWMASEGAA
                     APAPGTQDTRFAGWIEAASNAAEANGAGFWSVCGAPPSGGQGFTSDLVQVSPAALPID
                     PELTFR"
BASE COUNT         1139 a         2376 c         2462 g         1234 t
ORIGIN      
        1 cgaagtgcga agagctgatc taccagcccg aactcgagca caacatgcgg atgtgcccga
       61 aatgcggcta tttgcaccgc atgtccgccc ggcagcgaat cgagctgctg gccgatcctg
      121 attcgtttca ggaatgggac accgagcttc gcaccgccaa cccactcggc ttctcggaac
      181 cctcgggcag ctacgacagc aagatcgcca gaaccatggc ccgctccggc gagcaggaat
      241 cgctcatcac cggccggatg acggtcagcg gctacggcgt tgcgatcacc gtcgccgatt
      301 tcagcttcct cggcgcgagc atgggctccg tcttcggcga gaagctggcc cgggcggccg
      361 agcgcgccct cgacgagcgg ttgccggtcc tgaccgtcaa cgcgtccggc ggcgcccgca
      421 tgcacgaggg gctcttctcc ctgatgcaga tggccaagac gatcgccgcg ctgcggttgc
      481 tcgggggtgc ccggctgccg catatctcgc tgctgaccga cccctgctac ggcggcgtca
      541 gcgccagcta cgcgatggtg gccgacgtga tcctggccga gccgggcgcg ctggtcggct
      601 tcgcggggcc gcgggtgatc gagcagatca ccaagcaaaa gctgcccgac ggcttccaga
      661 ccgccgagtt cctgctcgag cacggcatga tcgatggcat cgtcgcccgg cccgatctcc
      721 cgaccgagct ggcccagctc cttgcccatt acagccgaat tcgcccgtcc gacccgatcc
      781 tcgtcaccag cgacgaaccg gggcgaaccg tcgagagtcc ggtgccggcc atcgagctgg
      841 cgcacctgaa cggcggggtc cgcgcgtgag cagcgttgtc aatcgtcgca cgcgccgccc
      901 aggcaagccg tccgaacccg agatcgtcga accgggcaat cccggcggcc tgaccgcatg
      961 ggaccgggtc aagctggccc gccatcccga ccggccgcac gcgctcgact acatctccga
     1021 gctgacgacc gatttcgtcg agctgcacgg ggatcgctcc ttcggcgacg accacgcgct
     1081 gatcggcggc ctggcgaatt tcgccggccg caccgtcatg gttctcggcc accagaaggg
     1141 gcgggacacg aaggagaata tccagcgcaa cttcggcatg gcccggcccg aggggtaccg
     1201 caaggccgag cggctgatgc gtcacgccga gaagttcggc atgcccgtca tcaccttcat
     1261 cgacacgccg ggcgccgagc cgggcatcgg ctccgaagag cgcggccaga gcacagcgat
     1321 cgccgagagc ctgctgacca tggccgggtt gcggacgatg gtcgtcgcgg tcgtgatcgg
     1381 cgagggcggc agcggcggcg cactggcgat cggtgtcgcc gaccgggtcc tgatgctcga
     1441 gaacgcgatc tactcggtgg catcccccga ggcctcggcg acgatcctct ggaaggacgt
     1501 ggcgaaggcg tcggacgcgg ccgcctcgat gaagatcacc gccctcgacc tctacggctt
     1561 cggcatgatt gacgagatca tccccgaggc gcacccggcc cacgagcgtc cgcgcgaggt
     1621 catcgccgcc gctggttcgg ccatccgccg ccatatcggc gacctccacg cccaaatcca
     1681 gcaggaaaac ggacctgacg agctcgtcca gcatcgctac gagaagttcc gccgcatcgg
     1741 atcctttacg gagtcgtgag ccggttgatg gtcgttggtc gttggtagat ggatacggaa
     1801 aaccagagac gaaagactag agaccaatga cctacgttgc gctcgagatc ggccggaagc
     1861 gcgtcattgc gtccgcgttg gagtggcccg gctgggctcg ggttgggcgc gatgagtatg
     1921 cggcgctcga gacgttgcgc gtctatgggc cgcggtatca acgggcgatc gcctcagtag
     1981 cttccgattt tcggatcccc gaacgggtgg aggagttcgt cgttgccgaa cggctgactg
     2041 gcgacgccac gaccgactac ggcgtgccgg tcatcgcgcc gtctgccgat gccgcgccgc
     2101 tggatgacgc cgccatgtat cgtctccgcg cgattctcgg cgcgtgctgg gatgcgctcg
     2161 agcgggctgc cgcatcggtc gagggcaagg aactgcgcac gggtccccgc ggtggcggcc
     2221 ggtccacacc gcagatcctg gaccacgtca tcgacgctca tttcggctac ttgaagcgac
     2281 tcgcctggcg tgagccgcac gagcgcatcg acgatctctc caccgaattc gccgcgatca
     2341 cggagatcaa tgccagcgcg ctcgccttcg caatgtcggg tgaaatgccg gcaacaggac
     2401 cgcgcggcgg caagctctgg accccgcgct acttcatccg ccgcgccgcc tggcacatcc
     2461 tcgaccacgc ctgggaaatc gaagaccgcc tggacgcctg aagccaatac ccgctcaggc
     2521 gatatgaaga tgacgaatgc gctggcgcat ttcgcgccgg cttgccgttc gccggtgacc
     2581 cgtcggccgc cgagtcttga gttcatgcac tgttgtgttg atgtcggatt ggggccggcc
     2641 cgggaggtca tagatcgaga tgtggaagcc gcgcgcttct cgttcagccc gattaagccc
     2701 ggctacatct ccgtgttgat tttccagatc gcgcgtcgcg aggatcgctc gctgggcagg
     2761 agtgagtcga atccggggag aagcaggatc cggatggttg cccaatgccc tgttccccgc
     2821 gatgtccgtg gccgtgcgcg tttgtctgtt tcggctcaca tccaacccca ctcgtgaaag
     2881 atctgctcga tcgctccacc gatcggcttg aaatctaccg gatcccgata cgtccaactt
     2941 cgaagcgtgt tctccagcct gctttagcgg gcttattgga ttcagcccgg gtgtttacgc
     3001 ccgggcggtc ccgggcacga acgctcacat gtgaccgaac ctatcgggga gcggcgggtt
     3061 tgcccccgca aacccctaca atcgtcgcgc ctccgcgcct cacaggtgcc gcaggaagag
     3121 gtccgggtcc atcaaaaagg tgcgggtcat ttggacatgc tcggtgtcgt cccaggtttg
     3181 ctcgcggatg ccgtgctcgc tcagatacag gatttgcgcg ccgggatagg ccatcagcat
     3241 cggcgcgtgg gtcgcgatga tgaactgcgc atcctgaccg actacctcct tcatcagcgc
     3301 cagcagcgcg atctgccgtt gtggggagag ggcgacctcc ggctcgtcca gcaggtagag
     3361 gccgccggga gtgaaccgct cggcgaagac ctggaagaac ccctcaccat gcgaccgggc
     3421 gttgaggtcg ccgtacttcc ggtcgagcgc gttccgctgg gccatcaccg ccgcgtgagc
     3481 gtaggtgccc ggctcaagcg tgctcgcgat ctgatcgagc tccgcgatcg tccgctgcgt
     3541 gctcaaaccg aagccgaaga agtcctccga gcgcatgtag aagccgcgat ggttccggct
     3601 cgaccagctc agcacgaggt ggcggcccag ctcctgcgcg gccgaaagcg tctcgtcccg
     3661 ctcgagctcg actgagccga tcgtcggcaa attggcggag gcggcgatcc cctcgagcag
     3721 ggtcgacttc ccgctcccgt tctccccgac caggaacgcg accggcgcgg tcagctccag
     3781 ctcgtcaagc gccaggatga tcggcaggtc gaacgggtat ccctccggca gcgcctcagg
     3841 gttgcggatc cggaccgagc gcaggtgcag catggacgta gtttagaagg cgcggaggcg
     3901 cggaggcgcg aagcaatgcg agcaggcaag cggacagggg atggaacggt agccaaatcc
     3961 gaaaaccgaa aaccgaaaac cgaaaaccaa cgggtacact cgccccgggg ttttcgcaat
     4021 gcgaggaaag tgagcgctaa tgactgtcgt tccggagttg cttgcggcga atgagtgcta
     4081 tgccgcgcag ttcacgtatg gagatctgcc cgtgccgccc gccaagcggc tcgtggtgct
     4141 gacctgcatg gacgcgcgga tcgacccgct ccgggcgctc ggcctcaagg aaggcgatgc
     4201 gcacgtcctc cgcaattccg gcggccgccc gacgcccgac atgctccgct cgctggccgt
     4261 ctcgcaggag atgctgggca cgcgcgagat cgtggtgatc catcacaccg aatgcggctc
     4321 ggtccggatc acgaacgaac tgatgcgcga gcgactggcc gaaaacatcg gccgggacgc
     4381 cgcaatcgcc gccaacgtga tcgatttcct gcccttccgc gacctcgcgc aaagcgtccg
     4441 ggacgacctc gccgtcctcc gcgccacgcc cctcctccgc cccgaaaccg aagtctccgg
     4501 cttcatcttc gacgtcaaaa cgggcaggat ccacgcggtg gatagtgaat agtggctagt
     4561 ggacagtgat ttggcagccg gcagccggta ccctcagtcc caaaacccaa aacccaaaac
     4621 cctatcggaa cgtcagctct gggtcgatcg gcagggccgc cgggctgacc tggaccaggt
     4681 cgctcgtgaa gccctgcccg ccgctcggcg gcgcgccgca gacggaccag aatccggctc
     4741 cgttcgcctc ggccgcattc gaggcggcct cgatccagcc ggcaaagcgc gtgtcctgcg
     4801 tgcccggggc cggcgctgcc gccccctccg aggccatcca ggccgcgacc agcaccaggc
     4861 cgccttcgcc gttgtcgatc cagacatcgc gcgcgtaggt atcgccgtcc gcggtgaccc
     4921 ggttccggaa ctcccgctcg aggtagaccg tcttgccgag gacgatgttg ctgttggtct
     4981 gcgtcgaatc agccgtgaag cactggttgc cggtcggtac attgacgccg aggtactgga
     5041 tcgtcgcgct cgtgccgtcc gcctcgacga cgatcgtgcg gccatcaatc acctgggtca
     5101 ccgtcgctgc gattagatcc gggcggaccg gcgccttgcc gagcttcgtc acgtcgccac
     5161 ccgccgccaa tgaagcgcca atgttcagac cggaggtgaa gtcgatcagc tcgatgttcg
     5221 tggtcgacgg cgggatcagg tagaccagcg ccaggcgaat cccctcgccc gccttgatcg
     5281 accgcaactc agtcgtttcg aggatgggat cgaagccgag gaagcgggcg atatccgggc
     5341 tgctcaggtc catccccacg aattgccagc cgaaatcgcc gctcacgacg agctggaaat
     5401 cggccatgtt gagcgtggcc ggctgttccg tccagttctg cgcatagagg acgacggtga
     5461 cccactcctg cccggggacc tcggtcagat cgagcgtcgg caggctcgcg ccgcgctcgg
     5521 cgaactcgac ggtgtagcgg aaggcgccgg tcgagaagac ctggtcgggt tcttcgccat
     5581 cgcgcaccga ccgctcctgc gccgggaagc ccgccttcgg cgtcggttcc ggcgtctcgg
     5641 tggcggtcgg ctcgggcgtg ttcgtggccg tcggcggcgt ggtcgcggtc gccgtggcgg
     5701 tcggaaccgc cgtttcggtc gccgtggccg ttggagtatc ggtcggcacg gtcgtcggcg
     5761 tctcggtcgg aacctcggta ttggtcgcgg ttggctcgac cgtcggcgtt tcagtgggat
     5821 ggtcggaggt cggatccggc ggcccgaaga ccggctcctc ggtggccgtg gcggtcggcg
     5881 acggctcggg cgtgttggtc ggttccggcg tcggggtatc cgtcgcggtc ggctcgggcg
     5941 atggcgtatc ggtggctgtg ggctcgaccg tcggagtctc ggttgcggtg ggttccggcg
     6001 tcggtgtgtc cgttgcggtc ggctcaggcg tcggggtatc ggccgccgtc ggttcagcag
     6061 tcgcggtgtc cgtcgcggtc gactccgggg tatccgttgc tgtcgcggcc ggttcgggcg
     6121 tatctgttgc cgtcgccgtt ggctcgggcg tgtcggtcgc ggttggttcc agcgttggcg
     6181 tctcagtcgg cgcgagggtc ggttcgtccg tcgcctcagc cgcggccagc gttggccagg
     6241 tcggcgccgt cttcgtaacg gtgggcctgg gcttcggcgt ggcggttgcg gtcggcgatg
     6301 ccgtattggt cgcggtcgct gccggcacgg atggagtagc ggatgcggcc ggggccgtct
     6361 cgccgccgac gccgatcgcg atcgccgtct cggcggcctc gcgggtcggc gcgttgacgc
     6421 catcgatgac cggcacggtc gcggtactgg tcgggatgac ctgcgcgacc gagccgcccg
     6481 gcgtctgctg cgtttgcggc gcgatgatcg gatcgaccag gcgcgaggtg tcgggcaggc
     6541 gattgccgcc gaaggccgcc gcaaaggcga tcagcgcgat cagcccggag aggcggaacg
     6601 cccagcccgc cttgttgccc ttgctggagc gcggcatcag ggccgggatc ggaatcagcg
     6661 accaatcgcc gccgcgatgc gtctggttgg cgagggtcga gatcgtgatc ggatcgccgg
     6721 agacgcgccc gccctcgacc acgctgccgt ggtgcgtcga cggggtctgg gtttcgagga
     6781 ccggcggaaa atcggtcgaa tccagcactt gcaacgtcgt cgcgtcgatg acgtcggcgg
     6841 tgtacttgtg ctcgtccgag cgttggaacg cctccggcgc cggttcgggc tcgcgggtca
     6901 ggtccggctg gtaggccgtg acccggcggc gttcggagcg gcggactggc ggctcgtcaa
     6961 aaatgggatc gagcggcacg gagggcagcg cctgcttgcc gtgggtcagc agcgtgacca
     7021 gctcggttgc cgcctgccgg ctgtcccggg ttgtgaactg gaggcgatcg cggtggccgg
     7081 agttcgtctc gatccggagg ttgaccgcgc cgttgccgct caggtagggc ggccgcttga
     7141 gccaccaccg gacgatgtcg agcacgacct cgccgccccg ggcgaaggcg agccgctgcg
     7201 aggtgatgat c
//
LOCUS       DASSGX010000104         2519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_563866, whole genome shotgun sequence.
ACCESSION   DASSGX010000104 DASSGX010000000
VERSION     DASSGX010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563866"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(231..1010)
                     /locus_tag="VFI12_01850"
     CDS             complement(231..1010)
                     /locus_tag="VFI12_01850"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HET7055172.1"
                     /translation="MTAIRYSRVLLIIAAALLVIAAGPVSVSAITGGGPDNGAHPYVG
                     MTYNDEYLCSGTLISATVFLTAGHCTALFQSGGSQVYVTFGDSVDFDPANAVTGTPYT
                     HPNFCLECAPGLPGFDAYDVGVIVLDQPVTGLGFGALPYESQVNDLAKGTALTAVGYG
                     VQGYERGGGQPQPIFTATRYRADVTFRNVDNRIGDMFIKLSNNQKGGTCSGDSGGPIF
                     APDQVTVLGVTAFGNGLCTSNGYAQRTDRADILAFIASFMQ"
     gene            1280..2227
                     /locus_tag="VFI12_01855"
     CDS             1280..2227
                     /locus_tag="VFI12_01855"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7055173.1"
                     /translation="MALTFAPTAPAPTASTSDLAIETRDLSKHYDKQIAVDHLNLQVR
                     QGEVYGFLGPNGAGKTTTLRMLLGLIQPTSGSATVAGGEPGSAESLDTVGAMIEQPGF
                     YPYMSGAANLRLLASYARVDPSRVETVLRMVDLDKRGDDKFSAYSQGMKQRLGLAAAL
                     LKGPAVLILDEPSNGLDPAGMAEMRVLIRALADEGRTVLLSSHLLGEIEQICDRIGVI
                     QKGRLVAEGTVDDLRGRESLSITALPLDAARAYLAERPDAELLGVSGASIQVKTDRSR
                     TADITRGLVHAGFDVTELSWRRQSLEDVFLGLTNEREGA"
     gene            2227..>2519
                     /locus_tag="VFI12_01860"
     CDS             2227..>2519
                     /locus_tag="VFI12_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055174.1"
                     /translation="MTRTILAETMALGKRPATWILLGLWNIMAVMFGYVIPYVSRNDA
                     AGVAGIRDLLPDQVTTTVLSGFPFFGGVIALLLAVMTFGGDYGWNVVKTLTTQR"
BASE COUNT          455 a          849 c          774 g          441 t
ORIGIN      
        1 ttgatcacgg tccgcacgcc caaatccgca taaaaaccca tctgccgcct gtccctgccc
       61 gcgcaattgc cccgcttcgg cattatctcg cagcggccaa ggctttgccg ggactccagt
      121 taaccgctcg ctgcaccggg aaactgcccg cttcccgggc agcacgaaga gaaacgggca
      181 ctggctttgg cgccagcacc cgttgcccgt atacggtcaa actcctgatg ctactgcatg
      241 aacgaggcga tgaatgcgag gatgtctgcc cggtcggtgc gctgcgcgta accgttcgag
      301 gtgcaaagcc cgttaccgaa tgcggtaacg ccgagaaccg tcacctggtc gggggcgaag
      361 atcgggccgc ccgagtctcc agagcaggtt ccacccttct gattattcga aagcttgatg
      421 aacatgtcgc cgatgcggtt atccacgttc cggaacgtga cgtcggcgcg atatcgggtc
      481 gcggtgaaga tcggctgcgg ctgaccgccg ccacgctcat acccctggac gccgtaccca
      541 acggcagtca gggccgtgcc cttcgccaga tcgttgacct ggctctcgta cggaagcgcg
      601 ccaaagccca ggccggttac cggctggtcg agcacgatca cgccgacgtc gtacgcgtcg
      661 aaaccgggca accccggtgc gcactcgagg cagaagttcg gatgcgtgta cggcgtgccc
      721 gtcaccgcgt tggccggatc gaagtccacg ctgtcaccga atgtcacgta cacctgtgag
      781 ccgcccgact ggaagagcgc cgtgcagtgg cccgcggtca ggaaaacggt ggccgagatg
      841 agcgtgcctg agcaaaggta ttcgtcgttg tacgtcatgc cgacgtacgg atgcgcccca
      901 ttgtccggtc cgccccccgt gatcgccgat accgataccg gccccgcggc aatcaccagg
      961 agcgcagcag cgatgatgag aagaacgcgg gaatatcgaa tagctgtcat tggtattcct
     1021 ccctgcgcgt tgactttccc gacccctgga gcccaagtgg ggtgccgcca ttctaggaag
     1081 cgatttgccg gtacacaatt gcgcaacgcc tgagctttgt aacgcgaact acgtgctctg
     1141 cagccggcac gcactgggcc aatggcgcga accgcaccca cttcatgagt tctccataca
     1201 tggttcgcga ccgcttcacg cgcgacgtcc attcttgatc cagagcgaac ttgcgctcga
     1261 tacacagaag gaaccgcgca tggcccttac ctttgctccg acagccccgg ctccgaccgc
     1321 gtcgacgtcg gacctggcaa ttgagacccg ggacctcagc aagcactacg acaagcaaat
     1381 cgccgtcgac cacttgaatt tgcaggttcg gcagggcgag gtctacggct tcctcgggcc
     1441 gaacggcgcc ggcaagacga cgacgctgcg gatgctgctc ggcctgatcc aacccacatc
     1501 cggatctgcg accgtcgccg gcggcgagcc cggtagcgct gagagtctcg acaccgtcgg
     1561 cgcgatgatc gagcaacccg gcttctatcc atatatgtcg ggcgcggcca acctgcgcct
     1621 gctggcgagc tacgcgcggg tcgatccctc gcgggtcgag acggtcctgc ggatggtcga
     1681 cctcgacaag cgcggcgacg acaagttcag cgcctattcg cagggcatga agcagcggct
     1741 cggcctggcc gcggcgctgc tcaagggccc ggctgtcctc atcctcgacg agccgtcgaa
     1801 cggcctggat ccggcgggca tggccgagat gcgggtgtta atccgcgccc tggccgatga
     1861 gggccggacc gtcctgctct ccagccacct cctcggagag atcgagcaga tctgcgaccg
     1921 catcggcgtt atccagaagg gccggctggt tgcggagggc acggtcgacg accttcgcgg
     1981 tcgggagtcg ctctcgatta ccgcgctccc actcgatgcc gcccgcgcct acctcgcgga
     2041 gcggccggat gcggagctgc tcggcgtatc cggcgcgtcc atccaggtca aaaccgatcg
     2101 gtcccgtacc gccgacatca cccgcggcct ggtccacgcc ggcttcgatg tgacggagct
     2161 gagctggcgc cggcaatcac tcgaggatgt ctttctcggc ctgaccaatg aacgggaggg
     2221 tgcctgatga cacgaaccat cctggccgag accatggccc tcggcaaacg ccccgccacg
     2281 tggatcctgc tcgggctctg gaacatcatg gccgtcatgt tcggctatgt catcccctac
     2341 gtcagccgga acgatgccgc gggtgtcgcc ggcatccgcg atctcctgcc ggaccaggtc
     2401 acgacgaccg tcctgagcgg ctttcccttc ttcggcggcg tgatcgcgct gctcctcgcc
     2461 gtgatgacct tcggcggcga ctacggctgg aatgtggtca agacgctgac gacccagcg
//
LOCUS       DASSGX010000105         3661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1326523, whole genome shotgun sequence.
ACCESSION   DASSGX010000105 DASSGX010000000
VERSION     DASSGX010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1326523"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="VFI12_01865"
     CDS             complement(<1..859)
                     /locus_tag="VFI12_01865"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HET7055175.1"
                     /translation="MSQHEAMSQPIRSELSVPGGDRRKIEKALESDADAVFLDLEDSV
                     APDQKDAARDVVVRALSELDWGRLPKAVRVNAPSTKWCYRDLIEVIEGAGTDLDKVVV
                     PKVRSAADIWFVHRLLAQLELQVCPTDPIRIEVQIEDAAGLMSLREIAGASSRISELT
                     FGQGDFAAATGMPAADIGVVDEWDNFVDGDRWLLPRQMIVFAARAAGIRALNGPYAAY
                     QHGEGFRGYCLMSRALGFDGVWCIHPNQIAIANDAFAPSPEQIERASRTVSALSDAWS
                     GGRGAVNRNG"
     gene            913..1206
                     /locus_tag="VFI12_01870"
     CDS             913..1206
                     /locus_tag="VFI12_01870"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HET7055176.1"
                     /translation="MFAVIARFKVKPGHVDEVIALLNQAAVPSREEPGCHLYIANRSL
                     DDPDLIVMYEQYDSPEAFQRHLESDHTREIVAGKVIPLLESRQRETYTVMNTG"
     gene            1216..1416
                     /locus_tag="VFI12_01875"
     CDS             1216..1416
                     /locus_tag="VFI12_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055177.1"
                     /translation="MAPPQPPDRRSGLHHAIVVCMATALFFLLWLGVTVIGDSARNSA
                     LVAFTLTLIFVVSLYWSPRSSR"
     gene            complement(1408..1875)
                     /locus_tag="VFI12_01880"
     CDS             complement(1408..1875)
                     /locus_tag="VFI12_01880"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7055178.1"
                     /translation="MGGTEKREATGSALGSWIRERRMRRRLSQKDLAAHAKISRSYLC
                     DIEQGRGTQPSLHVLQSIARSLGEDPAELMMQAGVDIDRADDLHPGSHRERRVLTMFR
                     ALSPAAQETIERFTRFLHDEEHRYIQPTLTPIANRPQPTAPQPSLSRIDEESA"
     gene            complement(1897..2919)
                     /locus_tag="VFI12_01885"
     CDS             complement(1897..2919)
                     /locus_tag="VFI12_01885"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HET7055179.1"
                     /translation="MAAPNSAMSAAYRAIDQRLKAGEVVILDGGIGSELQEVGFPEDP
                     HDRPANFTWGSIAINRAPEKLVEVHRRYAAAGADVLETHTFGLNRVWAATQDGRIDLP
                     KDAWKEMALESVRLVREGARQAGREDAAVAFACRTQDWPANQQEKAHDYDGYYVPLDM
                     DAYLRPLAELLANADAESRPDLILMEIQSWIPEDLQFPDYQLFLETGIPLWIAYRRTI
                     GQLVGVEGETIIEDGDRFGRAARKFEDMGASAVLVNCLPPRQIAGVGEWLRKFTSLPL
                     GAYPNMGTYLDYEWDWSTCETPAQIVEMARGWAQEGIQIIGGCCGAQPAHIRALAEAF
                     AAPAVV"
     gene            3042..>3661
                     /locus_tag="VFI12_01890"
     CDS             3042..>3661
                     /locus_tag="VFI12_01890"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7055180.1"
                     /translation="MTDLAPDQTLLPIDSPDDQADLARRHRESLFPSVGLYYPEPIEL
                     RRGERQYVYDGAGQQYLDFFGGIVTVSSGHAIPEINEPIKEQLDRMIHSSTLFLIRAQ
                     IELAERIKAMTPEKLNRVFFTNSGTEANEAAFLITTLNQSSNELIALRHSYHGRSFAT
                     INASGQRGWRSSTLSPLHVHYVGNPYCYRCPWEKTYPSCDLLCARDV"
BASE COUNT          625 a         1294 c         1081 g          661 t
ORIGIN      
        1 cgccgttccg gttgaccgct ccccgtccgc ccgaccacgc gtcgctcagc gcggacaccg
       61 tccggctggc tcgctcgatt tgctccggac tcggcgcaaa ggcgtcgttc gcgatcgcga
      121 tctggttcgg gtggatgcac cagacgccgt cgaatccgag cgcgcgcgac atcaggcagt
      181 agccgcgaaa cccctcgccg tgctggtacg ccgcgtacgg cccgttcagc gcccggatcc
      241 ctgccgcgcg agccgcgaag acgatcatct ggcgcggcaa caaccaccga tcgccgtcca
      301 cgaagttgtc ccattcgtcg acaaccccga tgtcagcggc cggcatgcct gtggccgccg
      361 cgaagtcccc ttggccgaag gtcagctcgc tgatccgtga cgacgctccg gcgatctcgc
      421 gaagggacat gagtcccgcc gcatcttcga tctgcacctc gatccgaatc ggatcagtcg
      481 ggcagacctg caactcgagt tgcgccagga ggcggtgaac gaaccagatg tcagcagccg
      541 aacgaacttt cggcaccacc accttgtcca ggtccgtccc ggcgccttcg atgacttcaa
      601 tcaggtcccg gtagcaccat ttcgtgctcg gcgcattgac ccggaccgcc ttcgggagcc
      661 ggccccaatc cagctccgaa agcgcgcgca cgacgacatc gcgggccgcg tccttttggt
      721 ccggggcgac cgaatcttcg agatcaagga agacggcgtc ggcgtcagac tcgagcgcct
      781 tctcgatctt ccggcgatct ccgcccggca ccgacaattc gctgcggatc ggctgcgaca
      841 tcgcttcatg ctggctcatg cgagaatctt cccacgaact gaacatcgat tcgattgggg
      901 agcataccgc tggtgtttgc cgtcattgcc cgtttcaagg tcaaacccgg acatgtggac
      961 gaggtgatcg cgctgctgaa tcaggccgcg gttccctccc gggaagagcc cggctgccac
     1021 ctctacatcg ccaaccgatc gctcgacgac ccggacctga tcgtcatgta cgagcaatac
     1081 gactcccccg aggcgttcca gcgccacctg gaatccgacc atacccggga gatcgtggcc
     1141 ggtaaggtca ttccgctgct cgaatcgcgc cagcgcgaga cgtacaccgt catgaacacc
     1201 ggatagacgc gttcgatggc cccgccgcag ccgccagacc gacgctccgg cctgcaccac
     1261 gctattgtcg tctgcatggc aacggcgctc ttcttcctgc tctggctggg ggtgaccgtg
     1321 attggcgaca gcgcccgcaa ctcggccctg gtcgccttca ccctgacgct gatcttcgtc
     1381 gtcagcctct actggtcgcc gcgttcctca cgctgactcc tcgtcgattc gtgatagcga
     1441 gggctgcggc gccgtgggct gcggccgatt ggcgatgggc gtcaacgtcg gctgaatgta
     1501 gcggtgctcc tcgtcgtgca ggaagcgcgt gaagcgttcg atggtctcct gagctgcggg
     1561 cgagagggcg cggaacatgg tcaacacccg tcgttcccgg tgcgacccgg ggtgaaggtc
     1621 atcggcccgg tcaatatcga ctccagcctg catcatgagt tcggccggat cctcgccgag
     1681 cgacctggcg atggactgca gcacgtgcag gctcggctgc gtaccgcgtc cctgctcaat
     1741 gtcgcagaga taggaacgac tgatcttcgc gtgcgccgcc aggtccttct ggctcagtcg
     1801 ccgtcgcatc cgccgctccc ggatccagct cccgagtgcc gatccggtcg cctctcgctt
     1861 ttccgtgccg cccatcctcc cgcctccctg acgccgctac acaaccgccg gggccgcaaa
     1921 cgcctcggcg agcgcccgga tatgggccgg ctgcgcccca cagcagccac cgatgatctg
     1981 gatgccctcc tgggcccaac cccgcgccat ctcgacgatc tgcgccggcg tttcgcacgt
     2041 ggaccagtcc cattcatagt cgaggtacgt gcccatgttt ggatacgcgc cgagcggcag
     2101 actggtgaac ttccggagcc actctccgac gccagcaatc tgacgtgggg gcaggcaatt
     2161 gacgagcacc gccgaggcgc ccatgtcttc gaatttgcgc gcggcgcggc caaatcggtc
     2221 accatcctcg atgatcgtct cgccctcgac cccgaccagc tgtccaatcg tgcggcggta
     2281 ggcaatccag agcggaatcc cggtctcgag gaagagctgg taatcgggaa actgaagatc
     2341 ctccgggatc caggactgga tctccatcag aatcaggtcc ggcctgctct ccgcgtcggc
     2401 attggccaag agttcggcga gcggccggag atatgcgtcc atgtccagcg gcacataata
     2461 gccgtcgtag tcatgcgcct tctcctgctg gttcgccggc caatcctgcg tgcggcaggc
     2521 gaacgcgacc gcggcatcct cccgcccggc ctgacgagcc ccttccctga ccagccgaac
     2581 cgactcgagc gccatctcct tccaggcgtc cttcggcagg tcgatccggc cgtcctgcgt
     2641 cgccgcccag acccggttca ggccgaacgt gtgtgtttcc agcacatccg cgccggccgc
     2701 ggcataccgg cgatggacct cgaccaactt ttccggcgcc cggttgatcg cgatcgagcc
     2761 ccatgtgaag ttggccggcc gatcatgcgg gtcttcggga aagccgacct cctgtagctc
     2821 ggagccaatg ccgccatcga gaatgacgac ctcgcccgct ttcaatcgct ggtcaatcgc
     2881 ccggtacgcg gcgctcatcg cgctgttcgg tgccgccatg gaggctctcc ctgaattgat
     2941 gtccatcgca atgcgcgcat catatcggaa ccgcgccggt ttcgagcaag tgtgcggtat
     3001 gctcaggcat ccaatccgac tactcgccca ggatgttttc catgaccgat ctcgcgcccg
     3061 accagacgct gttgccgatc gactcgccgg acgaccaggc ggatctcgcc cggcggcacc
     3121 gcgagagcct ctttccgagc gtcggcctct actacccgga gccgatcgag ctccggcgcg
     3181 gcgagcgaca atacgtctac gacggcgcgg ggcagcaata cctcgacttc ttcggcggga
     3241 tcgtcacggt ctcctccggt cacgcaattc cggagatcaa cgagccgatc aaggaacagc
     3301 tcgaccgcat gatccactcg tcgactctgt tcctgatccg agcgcagatc gagctggccg
     3361 agcggatcaa ggcgatgacg cccgagaagc tgaaccgcgt ctttttcacg aacagcggca
     3421 ccgaggcgaa cgaggccgcc ttcctgatca cgaccctgaa ccagtcgagc aacgaactga
     3481 tcgccctccg ccactcctac cacggccggt cgttcgcgac gatcaatgcg tccggccagc
     3541 gcggttggcg atcgtccacg ctcagcccgc tccacgtcca ctacgtcggt aatccgtatt
     3601 gctaccgctg cccgtgggag aagacgtacc cgtcgtgcga tctgctctgc gcgcgggacg
     3661 t
//
LOCUS       DASSGX010000106         2146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_128887, whole genome shotgun sequence.
ACCESSION   DASSGX010000106 DASSGX010000000
VERSION     DASSGX010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128887"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VFI12_01895"
     CDS             <1..510
                     /locus_tag="VFI12_01895"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET7055181.1"
                     /translation="TGGAVIDMMIHDYDALNWVFGEPKAVVARGHRNPRSGGYDQVQV
                     MIDYGNAWAQVDGGMMMPESYPFTSSLQVLCETGAMEYDFQAGGRSVEMGAGRNELKL
                     FPNEGDPCQVTVEQADPYAAEVAYWVDCIRTGQPATRATPTDARTALKVALAARRSLD
                     NNGLVAVPV"
     gene            complement(515..748)
                     /locus_tag="VFI12_01900"
     CDS             complement(515..748)
                     /locus_tag="VFI12_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055182.1"
                     /translation="MIWPETGLQVTTTAPVKEKDTLTENDQIELSLDFVTLSLTPTEF
                     IQLAASLRLSVDGLLDHHPGLQRAVVAAFDIKM"
     gene            973..1539
                     /locus_tag="VFI12_01905"
     CDS             973..1539
                     /locus_tag="VFI12_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055183.1"
                     /translation="MSDRDALANELRQMIEHIRAHVPDAVVNTRLAQSDQHGVVMHAQ
                     IMGGSGPSGSAHASAPIGDCAPEVAENRALARAMIAMGLPSMPDAAPQKETTPAPKLH
                     SVADQPEQPGRVVQFPAHEPERPEPKPEQPESDPEPEDISWTAFWTWAKANGFANRDA
                     LEAAIGQSINQLTPAQIRKLVQSATDTR"
BASE COUNT          392 a          772 c          640 g          342 t
ORIGIN      
        1 accggcgggg ccgtcatcga catgatgatc cacgattacg acgccctgaa ctgggtcttc
       61 ggcgagccga aagcggttgt cgcgcggggc caccgcaacc cgcggtcggg cggatacgac
      121 caggtccagg tcatgatcga ctacggcaat gcctgggcgc aggtcgatgg cggcatgatg
      181 atgcccgaga gctacccgtt cacctcatcg ctccaggtgc tctgcgagac tggcgccatg
      241 gaatacgact tccaggccgg cggtcgcagc gtcgagatgg gcgccggccg gaatgagctg
      301 aagctcttcc cgaacgaggg cgatccctgc caggtcaccg tcgagcaggc cgatccctac
      361 gccgccgagg tcgcctactg ggttgactgc atccggaccg ggcagccggc cactcgcgcc
      421 accccaaccg acgcccggac cgccctcaag gtcgccctgg ccgcgcggcg ctccctcgac
      481 aataacggcc tcgtcgccgt gccggtctga gtcgctacat cttgatgtcg aacgccgcga
      541 ccaccgcccg ctgcaacccc gggtggtggt ccagcaaccc gtccacactc aaccgcaggc
      601 tggcggcgag ctggatgaac tcggtcggcg tcaacgagag cgtcacgaaa tcgaggctca
      661 gctcgatctg atcattctcg gtcagcgtgt ccttctcctt gaccggcgcc gtcgtcgtca
      721 cctgcaaccc cgtctccggc caaatcatgt ccagcaaatc aatccgcgct cgattcacag
      781 ccattccccc atgcaattca cacgcattgt gccgtgaatc agccgggaca cgccgatcgc
      841 cggtgaggta gcgctcacga ctcacgtctc acgactcatg acttgagctt gcctctctct
      901 tccgaactga tgtactgact ctgtatactc agaggatcaa ctggttgggg aatgggggat
      961 cggggattcg acgtgagcga ccgggacgca ctggccaatg agcttcgcca gatgatcgag
     1021 cacatccgtg cccacgtgcc cgatgccgtc gtgaataccc ggcttgccca gagcgaccag
     1081 cacggcgtcg tcatgcacgc ccagatcatg ggcggctctg gcccgtccgg ctcggctcat
     1141 gccagtgcgc cgattggcga ctgtgcgccc gaagttgccg agaatcgcgc ccttgcccgg
     1201 gccatgatcg cgatgggtct gccgtcgatg ccggacgccg ctccgcagaa ggagacgacg
     1261 cccgcgccca agcttcattc tgtcgccgat cagccagagc agcccggccg cgtcgtccaa
     1321 ttcccggccc acgaacccga gcgacccgag ccaaaaccgg aacaaccgga atctgacccg
     1381 gaaccggaag acatctcctg gaccgccttc tggacctggg ccaaggccaa cggcttcgct
     1441 aaccgcgacg ccctcgaagc cgccatcggc caatccatca accagctcac ccccgcccaa
     1501 atccgcaaac tcgtccaatc cgccaccgac acccgctgat tgctgccgtt tcacggagcg
     1561 cgcagagctg ccatgcgcgc aatcgcccgc ggcgcgatgt ccgtgtgccc tgccaagggc
     1621 caggattgca cgcactgaag caccggctca cgacaaggta gccccgggcg gcctcgcccg
     1681 gcttccctta acgaagatct ggtaaatgac accggtccca cgcccgaccg tcacagacta
     1741 accatgtggt cgtttccgcc ctgaataggg aacagcggag cggcggagtc ggtcggctcg
     1801 agctgacatc cgtatcgcgg atccggtaat cattccgggg cgatgattgc cgcggggtcc
     1861 cacgcagcgg atgccttggc cagacagcca ggcgaggccg tccggggcta cattttgagg
     1921 aacgcatgaa gaccggctga gccctgcgtg gcccgagttg tcgctccgcg cggcgcggag
     1981 ggcgtacgat agtgctcgct cacgccgccg gcacggcctc gccgcgctcg accatccggc
     2041 cccagagcgc cgcgccgatc acgcccgcct cataccccag cgccgccggc acgaacgaca
     2101 cgcgcgacat gtccgtgacc gtgttgctcg cccgcgcggt cgactc
//
LOCUS       DASSGX010000107         3246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_346984, whole genome shotgun sequence.
ACCESSION   DASSGX010000107 DASSGX010000000
VERSION     DASSGX010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3246
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346984"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VFI12_01910"
     CDS             <1..438
                     /locus_tag="VFI12_01910"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HET7055184.1"
                     /translation="EGMMYASTIAGMAFGNADVAAVHCISEAIGGLYDTPHGVANSVF
                     LPWVFDFNRAADPYRHADVAEALGIARDGRGDEAVAADGARLLEDLSERIGIPRFSGL
                     PGVDPADFDRLAEASEANGSTPSNARPITRADYRHILDAAYAG"
     gene            complement(474..878)
                     /locus_tag="VFI12_01915"
     CDS             complement(474..878)
                     /locus_tag="VFI12_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055185.1"
                     /translation="MLGEMIGEESGKIYGTRVLPYEGGATRIENSMRATGKLLGVEVT
                     NIATIWGAIRPDGTLYIEGQGIVMAKDGGSATFVGWGFGRFTANGGVSYRGGINCPNA
                     TGSLERLNSAIIPYEAEADADQNITWKFWEWK"
     gene            1168..>3246
                     /locus_tag="VFI12_01920"
     CDS             1168..>3246
                     /locus_tag="VFI12_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055186.1"
                     /translation="MSERSDRPVPDLLGCGLLAVTIALLFWNRATHDIWIARHDNLTA
                     YLPWWSYLGERLRAGEIPGWNPHQFSGIPFLADPQSGWMHLPAMISFILFDPLTAMKA
                     KVLIELLIAGYGTYALARVYGYGPIAAFAGAALFIFGPFSLFTTYCCTVRLHIATWIP
                     LALLGPEIALRSRRWHGRLAGIGIGAFAYSQMLAGWLGQGTYDAALIIGAYCLYRALT
                     LPAIGSRIRRALVGIATGLALGIFGAALNAAALLPRLAVNGETHLGAGNYDQLPSGYY
                     YNPFGIKQLVATLINDDYAHRGYTIPAAGLLLVLLALTLVPRAHPVPFFAGMTLVIYA
                     LTLNWGPPYWLLSLLPRWQELHDHYPQQAAAAVMIGPAMLAAAAVDALPRLHRFRFRA
                     PRIIIPVLVIGAGMAWIAATQDFNRPLLFPLIVLVVATLLISLLAIRRLPGTVAMPVL
                     VILGLLVFVEPMGLEIIDASTSGRIIANWYPFWEPDPSLAAAAKTAVNPHDPGGAGDF
                     LQQQMAADGPFRYVGYAGAGYPGDPNERVTYMERRAEPQIRAILVNGRSIFLDLYDAQ
                     GYNPTQLDRYVQYVTAMNGKQIDYHLAELRVGGIGSPLLDMLNVRYLLVDARLPLDRP
                     DVAALTGGEQPVFANRNVRVYENPHAFPHAWLIHDIRHVTQEQAFALLRDRAVDFRRV
                     ALVEGDAPAVT"
BASE COUNT          477 a         1132 c         1022 g          615 t
ORIGIN      
        1 gaggggatga tgtacgccag cacgatcgcc gggatggcgt tcggcaatgc ggacgtcgcg
       61 gcggtgcatt gcatctccga ggcgatcggc gggctctacg acaccccgca tggcgtcgcc
      121 aattcggtct tcctgccgtg ggtgttcgac ttcaacaggg cggccgatcc ctaccgccat
      181 gccgatgtcg ccgaggcgtt gggcatcgcg cgggatggtc gtggcgatga ggccgtcgcc
      241 gccgatggcg cgcgattgct cgaggactta tcggagcgga tcggcatccc gcgcttttcc
      301 gggttgccgg gcgtcgatcc ggccgatttc gaccggctgg ccgaggcaag cgaggcgaac
      361 ggcagcacgc cgagcaatgc ccgcccgatc acccgcgccg actaccgcca catcctcgac
      421 gccgcctatg ccggctgacg cggccctatc cagcaacagg agcgtaatcc ggactatttc
      481 cactcccaga atttccaggt gatgttttgg tccgcgtcgg cttcggcctc gtaggggatg
      541 attgcgctgt tgaggcgctc cagggagccc gtggcattcg ggcagttgat gccaccccga
      601 tagctcactc cgccgtttgc ggtgaatcgc ccgaagcccc agccaacgaa ggtggcgctg
      661 ccgccgtctt tggccatcac tatcccttgc ccctcgatat agagggtccc gtcgggccgg
      721 attgcgcccc agatggtcgc gatgtttgtt acctcgaccc cgagcagctt ccccgtggcc
      781 cgcatggagt tttcgatcct ggtggcgccg ccctcatatg gcagcacccg ggttccatag
      841 atctttccgg attcttcgcc gatcatctcg cctaacatgg gcttgagccc tcctcgttgc
      901 cgctaacgtg atgtcgaaga ccgcgatgcg cacgagagaa atccgccggg ctgcctcctt
      961 tccgccgggc cagcgtatca gatcagtgtg cgacacataa cgtgtacgta aagaatgagc
     1021 aggttggtcg tgcgcggtgt cgttgcgcag gcgattccct caactcccca ccttcaaccc
     1081 gttggtagcg cccggcatgt gcgatatccg ggcttccgtg ttgaatcggg gattgtttgc
     1141 tggcatccga tgcgcgggag aaggcgtgtg tccgagcggt ccgaccggcc ggttccagat
     1201 ctcctcggtt gcgggctcct ggccgtcacc atcgccctgc tcttctggaa ccgcgccacg
     1261 catgacatct ggatcgcccg gcacgacaac ctgacggcct acctgccctg gtggagctat
     1321 ctcggcgagc ggctccgtgc cggggagatc ccgggctgga acccgcatca gttcagcggc
     1381 atccccttcc tggccgatcc gcaatcgggc tggatgcatc tgccggcgat gatcagcttc
     1441 atcctcttcg atccgctgac cgcgatgaag gcgaaggtcc tgatcgagct gctgatcgcc
     1501 gggtacggga cctacgcgct ggcccgggtc tacggctacg ggccgatcgc cgccttcgcc
     1561 ggggcggcgc tcttcatctt cggcccgttc tcgctcttca cgacctattg ctgcacggtt
     1621 cggctccaca tcgcgacctg gatcccgctc gccctgctcg gcccggagat tgccctccgc
     1681 tcgcggcgct ggcatggtcg cctggccggg atcgggatcg gcgcgttcgc ctacagccag
     1741 atgctggccg gctggctcgg gcagggcacg tacgatgccg ccctgatcat cggcgcgtac
     1801 tgcctgtacc gggccctgac gctcccggcg atcggcagcc ggatccggcg ggcgctggtc
     1861 ggtattgcca caggtctcgc gctcggcatc ttcggggccg cgctcaatgc cgccgcgctc
     1921 ctgcccaggc tggcggtcaa tggggagacg cacctcggcg ccggcaatta cgatcaactc
     1981 cccagcggct attactacaa cccgttcggg atcaagcagt tggtcgcgac gctgatcaat
     2041 gacgactacg cgcaccgtgg ctacacgatt ccggcagccg ggctgctgct cgttctgctg
     2101 gcgcttaccc tcgtgccgcg cgcccacccg gtccccttct ttgccgggat gacgctggtc
     2161 atctacgcgc tgacgctcaa ctggggtccg ccctactggc tgctctcgct gctgccaagg
     2221 tggcaggagc tgcacgacca ctacccgcag caagcggccg cggccgtgat gatcggcccg
     2281 gccatgctgg cggcggcggc agtcgatgcc ttgccgcgtc tgcatcgttt cagattccgg
     2341 gccccacgga tcatcatccc ggtgctcgtg atcggggcgg ggatggcgtg gatcgccgcg
     2401 acccaggact tcaaccgacc actgctcttc ccgctgatcg tgctcgtggt tgccacactg
     2461 ctgatcagcc tgctcgcgat caggcggctg cccggcacgg tcgccatgcc ggtgctggtc
     2521 attctcgggc tgctcgtctt cgtcgagccg atggggctgg agatcatcga cgccagcacc
     2581 agcggccgga tcatcgccaa ctggtatccg ttctgggagc cggatcccag cctcgccgcc
     2641 gccgccaaaa cggccgtcaa cccgcacgat ccgggtggcg cgggcgattt cctccagcag
     2701 cagatggcgg ccgatggccc cttccgctac gtcggctacg cgggcgccgg ctatcccggc
     2761 gatccgaacg agcgggtgac ctacatggag cgtcgcgccg agccgcaaat ccgggccatc
     2821 ctcgtcaacg gccgctcgat cttcctcgac ctctacgacg cgcagggcta caacccgacc
     2881 cagctcgatc gctacgtcca gtacgtcacc gccatgaacg ggaagcagat cgattaccac
     2941 ctggccgagc tgcgcgttgg cggaatcggc tcgccgctcc tcgacatgct taacgtccgc
     3001 tacctcctgg tcgatgcgcg cctgccgctc gaccggccgg acgttgccgc gctgaccggg
     3061 ggagagcagc cggtcttcgc gaaccggaac gtgcgggtct acgagaaccc ccatgccttc
     3121 ccgcacgcct ggctgatcca tgacattcgc catgtcaccc aggagcaggc attcgccctg
     3181 ctgcgggacc gcgcggtcga tttccgtcgg gtggcgctgg tcgagggcga cgctccggcc
     3241 gtcacc
//
LOCUS       DASSGX010000108         3080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_891944, whole genome shotgun sequence.
ACCESSION   DASSGX010000108 DASSGX010000000
VERSION     DASSGX010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891944"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VFI12_01925"
     CDS             complement(<1..199)
                     /locus_tag="VFI12_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HET7055187.1"
                     /translation="MIIYPAIDIRGGKCVRLVEGDFDRETVFDQDPAEAARRWEAGGA
                     THIHVVDLDGARNGEPTSLDTI"
     gene            complement(196..831)
                     /gene="hisH"
                     /locus_tag="VFI12_01930"
     CDS             complement(196..831)
                     /gene="hisH"
                     /locus_tag="VFI12_01930"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HET7055188.1"
                     /translation="MIGVVDYGAGNIRSISRGLAAAGGDAVVAEHGRELAAADAIVLP
                     GVGHAGQLMNALQTRGFDTAILDAVAAGTPFLGICVGLQIMFGQQEEGNTRGLGLLPG
                     EVRHLPRTLKTPHMGWSQVTAVTDSAFGATGDSDYYYFVHSYAAYEAEPDTVVATTNY
                     GVTYPSVVIKDHLWGCQFHPEKSSNAGITFLAKFVDFARESASARKAVAAK"
     gene            complement(828..1433)
                     /gene="hisB"
                     /locus_tag="VFI12_01935"
     CDS             complement(828..1433)
                     /gene="hisB"
                     /locus_tag="VFI12_01935"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF002111.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HET7055189.1"
                     /translation="MTSLPIRTRSIERHTRETKISVTVDLDGSGEVDVETGVPFLDHM
                     LDALGRHGQLNLWVRCAGDVEIDPHHSVEDVGIVIGLATREALGDRAGIARYAHAYAP
                     LDEALSRTVIDVSGRPYFHFTGEMPEPQIGADFAASLIEEFWRAFAMNSGFTMHIDLL
                     RSRNSHHAAEAIFKSAALAIHDATRVSRSTMAIPSTKGLLG"
     gene            complement(1430..2533)
                     /gene="hisC"
                     /locus_tag="VFI12_01940"
     CDS             complement(1430..2533)
                     /gene="hisC"
                     /locus_tag="VFI12_01940"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HET7055190.1"
                     /translation="MTTSSTNTDLTALVRREIRNLPGYVPAAGAAKKPDRLLRLDMNE
                     SPYGPSPRTRAALADFVQTNRYPDFAQSALRSALSEYTGKPFEQIICGAGLDDVFTTL
                     ANLMIEPGDEVVISEPTFGVYRPLFSMHGAKVVNVPLTEDFAFQSDQVLAAVTDRTKI
                     VIICSPNNPTGNLFPIEAVEQVCANARCLVAIDEAYAEFAGHSHIPLMDRYPNVMILR
                     TMSKWAGLAGMRVGYGVIPEQLVGVVGHVVPPFHNVSLASSEAAIASIEDREFLMGRV
                     ADICTARDALFAELATIDGLAPLPSVTNFILVKTPYSDARPLVQAVAERGILIRGYGD
                     PLLQPYFRVSVGLPAENDQFLHALRDAIKEMPA"
     gene            complement(2545..>3080)
                     /gene="hisG"
                     /locus_tag="VFI12_01945"
     CDS             complement(2545..>3080)
                     /gene="hisG"
                     /locus_tag="VFI12_01945"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR03455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HET7055191.1"
                     /translation="KSTKLATSYPKSARRFFAERDAHPEIITLSGSVEMAPGLGLAEG
                     IVELTATGSTLILNDLRQIATILESEAVLVANKASLQDPEKKHQIDRLMLRINAVRAA
                     RRFKYLMMNAPESALDNIKRIVPGLKSPTVIPLQIPGWVAVHMAIQEDIFWESIEQLR
                     AAGATEILVSSLDKLLL"
BASE COUNT          544 a          983 c         1007 g          546 t
ORIGIN      
        1 taatcgtgtc caggcttgtt ggctcgccgt tccgagcgcc gtcaaggtcg accacgtgga
       61 tatgcgtcgc tccgccggct tcccaccggc gtgcggcctc ggcagggtcc tggtcgaaga
      121 cagtctcgcg gtcaaagtcg ccttccacga ggcgcacgca cttgccgccc cgaatgtcga
      181 ttgccgggta gatgatcact tggccgcgac tgcctttcgc gccgaagccg actctcgcgc
      241 gaagtccacg aacttcgcca ggaatgtgat gcccgcgttc gagctcttct cggggtggaa
      301 ctggcaaccc cagaggtgat ccttgatgac gacgcttgga taggtaacgc cgtaattggt
      361 cgtcgcgacg accgtgtccg gctctgcctc atacgccgca tagctgtgga cgaaatagta
      421 gtagtccgaa tcacccgtcg caccgaaagc gctgtcggtg accgcggtca cctggctcca
      481 gcccatgtgc ggcgtcttga gcgtgcgcgg caggtgccgg acctcgcccg gaagcaggcc
      541 gaggccccgc gtattgccct cctcctgctg gccgaacatg atctgcagtc cgacgcaaat
      601 cccgaggaag ggcgtaccgg ccgcgaccgc atccagaatc gcggtgtcga acccgcgggt
      661 ctgcagcgcg ttcatcaatt gaccggcgtg cccgacgccc ggcagcacga tcgcatcggc
      721 cgccgccagt tcgcgcccgt gctccgcaac gacggcgtcg ccgcccgcgg cagccaggcc
      781 ccggctgatc gaccggatgt tgcccgctcc gtaatcgacg acgccgatca tcccagcagg
      841 cccttcgtcg acgggatcgc cattgtcgag cggctgaccc gggttgcatc atggatcgca
      901 agggcggcgg atttgaagat cgcctcggcc gcgtggtgcg agttgcgcga gcggaggagg
      961 tcgatgtgca tggtgaagcc ggaattcatc gcgaatgcgc gccagaactc ctcgatcagg
     1021 ctggccgcga aatcggcccc gatctgcggc tccggcatct cgccggtgaa atggaaatac
     1081 ggccggccgg agacgtcgat cacggtccgg gacagggcct catcgagcgg ggcataggcg
     1141 tgcgcatacc gggcgatccc ggcgcggtcg ccgagcgcct cccgtgttgc cagaccgatt
     1201 acgatgccga cgtcctcgac cgaatgatgc gggtcgatct cgacgtcgcc ggcgcagcgc
     1261 acccacaggt tcagttgccc gtgccgcccg agcgcgtcaa gcatatggtc gagaaatggc
     1321 acgccggttt cgacgtcgac ctctccgctc ccatccaggt cgaccgtcac cgagatcttc
     1381 gtttcgcggg tgtgccgctc gatcgagcgc gtcctgatcg gcagagaagt catgctggca
     1441 tctccttgat ggcgtcacgg agcgcgtgca ggaattggtc gttttctgcc ggcaggccaa
     1501 cggaaacccg gaaatatggc tggagcaggg gatcaccata accgcggatc aaaatgccgc
     1561 gctcggcaac cgcctggacc agcgggcgcg cgtcggaata cggggtcttg accaggatga
     1621 aattggtcac ggacggaagc ggcgccagcc catcaatcgt cgccagttcg gcgaacaggg
     1681 catcccgcgc cgtgcagatg tccgcgaccc ggcccatcag gaactcacga tcctcgatcg
     1741 acgcgattgc cgcctcggac gatgcgagcg agacattgtg gaacggcggc accacatgcc
     1801 cgacgacgcc gacaagctgc tctggaatga ccccgtaccc gacgcgcatc cccgccaagc
     1861 cggcccattt gctcatcgtg cgcagaatca tcacgttcgg ataccggtcc atcagcggaa
     1921 tgtgcgagtg accggcgaac tcggcatagg cctcgtcgat cgcgaccagg cagcgcgcgt
     1981 tggcgcagac ctgctcgacg gcctcgatcg ggaagaggtt gccggtcgga ttgttcggcg
     2041 agcagatgat gacgatcttc gtgcggtcgg taaccgccgc gagaacctgg tccgactgga
     2101 atgcgaaatc ctcggtcagg gggacgttaa ccacctttgc gccgtgcatc gagaagagcg
     2161 gccggtaaac cccgaatgtc ggctcactga tgaccacctc gtcgcccggc tcgatcatga
     2221 ggttcgcaag cgttgtgaag acgtcgtcga gccctgcgcc gcagatgatc tgctcgaacg
     2281 gtttgccggt gtattcggag agcgcactgc ggagagccga ctgcgcgaag tccgggtacc
     2341 ggttggtttg cacgaaatcg gccagggccg cacgggttcg cggggagggc ccatatggcg
     2401 actcgttcat gtccagccgc agcaaccgat ccggtttctt ggccgcgccg gcagcaggga
     2461 catagcccgg caggttgcgg atctcccgac gcacgagcgc ggtcaggtcg gtgttggtgg
     2521 atgacgtcgt catatcggtc gacctcagag caagagcttg tcgagcgatg agacgagaat
     2581 ctctgtcgcc ccggccgcgc ggagctgctc gatcgactcc cagaaaatgt cctcctggat
     2641 cgccatatgg accgcgaccc agcccggaat ctggagcggg atgaccgtcg gcgatttcag
     2701 gcccggcacg atccgcttga tattgtcgag ggccgattcg ggcgcgttca tcatcaggta
     2761 cttgaaccgg cgcgccgcgc ggaccgcatt gattcggagc atgagccgat caatctggtg
     2821 cttcttctcc gggtcctgga gcgacgcttt gttcgcgacc agcacggcct cggattcgag
     2881 gatcgttgcg atctggcgga ggtcgttcag gatcagcgtc gagccggtcg cggtcagctc
     2941 gacgatgccc tcggccagcc cgaggccggg cgccatctcg acggacccgc tgagcgtgat
     3001 gatctccggg tgggcgtccc tttcggcgaa gaatcgacgg gccgacttcg ggtaggacgt
     3061 ggcgagcttg gtcgatttga
//
LOCUS       DASSGX010000109         2963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_564829, whole genome shotgun sequence.
ACCESSION   DASSGX010000109 DASSGX010000000
VERSION     DASSGX010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564829"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="VFI12_01950"
     CDS             complement(<1..505)
                     /locus_tag="VFI12_01950"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HET7055192.1"
                     /translation="MAKVAIIGTGLIGRAWSMVFARAGWDVAVWDGVADVAGSALSLI
                     AQGLDDLKRHGLIDDPRAATARVRAAPSFEEAIAGADLVQENTPESLDIKREIFARLD
                     AAADSQTILASSTSSLVTSLYTEQLGGRSRCLVAHPVNPPHLVPIVELCGAPWTSKET
                     IARARAFY"
     gene            647..1423
                     /locus_tag="VFI12_01955"
     CDS             647..1423
                     /locus_tag="VFI12_01955"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7055193.1"
                     /translation="MDMQIDGLRVLVTAGAAGIGLETARAFVRENAKVHVCDVDRPAL
                     EALAKSDPKLTWSVCDVADRAQVAAMFDDALKQLGGLDCLVNNAGIAGPTGRVEEINP
                     EDWDRCLAVDITGQFNCVRLAVPHLKESKNASIMNLSSQAGKHGFPLRSAYAAAKWGV
                     VGFTKTIAMELGPFGIRANALLPGLVAGDRIRRVIEAKAQQRGVSFKEQEQHMMQYAS
                     LKEYVTPQQLADMIVFTASPCGRTISGQAISVCSDTNMLG"
     gene            1599..1672
                     /locus_tag="VFI12_01960"
     tRNA            1599..1672
                     /locus_tag="VFI12_01960"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1631..1633,aa:Gln,seq:ctg)
     gene            1986..>2963
                     /gene="argE"
                     /locus_tag="VFI12_01965"
     CDS             1986..>2963
                     /gene="argE"
                     /locus_tag="VFI12_01965"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF005710.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HET7055194.1"
                     /translation="MDSIRILEKLIALPTVSRDSNLDLIAHVSDTLRDHGIASRLVPS
                     QDGHKANLFATIGPQDRPGVMLSGHTDVVPVDGQNWTLPPFVMTERDGKLYGRGAADM
                     KGFVACALSAGIKASKIGLATPLHLAFSYDEEVGCLGVRSLIDLLQAAPHRPLLCIVG
                     EPTGMQVATGHKGKIAARALCRGREGHSALAPLALNAIHLGCDLIAVLRKEQDRLARE
                     GARDGDYDVPYTTVHVGRIDAGVALNIVPNLCQVDFEIRNVTDDDPQAILAGIRAEAA
                     RIAADASSLAQEAAIEIEVINSYPGLETPASSQAVAFVKSLTGANRTTKV"
BASE COUNT          531 a          928 c          956 g          548 t
ORIGIN      
        1 tgtagaaggc acgcgcgcgg gcgattgtct ctttgctcgt ccacggcgcg ccgcagagtt
       61 cgacgatcgg cacgagatgc ggcgggttca ccggatgcgc gacgagacag cggctgcgcc
      121 cgcccaattg ctccgtatag agcgatgtga cgagcgagga cgtcgacgaa gcaagaatgg
      181 tttgcgaatc cgcggctgcg tcgaggcgcg caaagatctc gcgcttgata tccaggcttt
      241 ccggtgtgtt ctcctgaacg aggtcggccc cggcgatcgc ctcctcgaag ctcggtgcag
      301 cgcgcacgcg ggcagtggcg gcacggggat catcgatcag tccgtgccgc tttaggtcat
      361 cgagtccctg cgcgatgagg ctcagcgcgc tgccggcgac gtccgcaacg ccatcccaca
      421 ccgcgacgtc ccagcccgcg cgcgcaaaca ccatcgacca ggcacggcct atcagccctg
      481 tcccgattat tgccactttt gccatgcgtt ccctctccgt cgtctcctat gcggatctcc
      541 gcagcgctcg cattccatcg cccctgggtt gggctgagtt gtttcaacct agccgcgcca
      601 tgcaataatg ggaatgaaga acctcagagc cgggaggagg gctgccatgg acatgcagat
      661 cgacggacta cgggtgctgg tgacggcggg agcggccggc atcggcctcg agaccgcccg
      721 cgcattcgta cgcgagaatg ccaaggtgca cgtttgcgat gtcgaccggc cggctctcga
      781 ggccctggcg aaatccgacc cgaagctgac atggagcgtg tgcgatgtcg ccgaccgggc
      841 ccaggtcgcg gccatgttcg acgacgcgct aaagcagctc ggcggtctcg actgtcttgt
      901 caacaacgcg ggcatcgccg ggccgacggg ccgcgtcgag gagatcaacc cggaggattg
      961 ggacaggtgc ctcgccgtcg acatcaccgg gcagttcaac tgcgtgcgac tggccgttcc
     1021 gcacctgaag gagagcaaga acgccagcat catgaacctg tcctcgcagg cgggtaagca
     1081 cgggtttccg ctccgttccg cctatgcggc agcgaaatgg ggtgtggtcg gcttcacgaa
     1141 gacgattgcc atggagctcg ggccgttcgg cattcgggcg aacgcgctgc ttccaggact
     1201 ggttgcgggc gaccgcatcc gtcgcgtcat cgaggccaag gcacagcagc gcggcgtctc
     1261 cttcaaggag caggagcagc acatgatgca gtatgcctcc ctcaaggagt acgtcacgcc
     1321 ccagcagctt gccgacatga tcgtgttcac ggccagcccc tgcggccgca ccatctctgg
     1381 ccaggccatc tcggtctgca gcgacaccaa catgctggga tgagactctg ccggggtgcg
     1441 acggctaggc gcacttgccg tgacgtgagc gtggatcgat ttcgcggttg agacgtttag
     1501 cggactgtcg attgcggggc ggcggctgcc ttgcggtccg caaggcaagc tgttatacgg
     1561 ccatctctcg gcccctggcg ctaaggccag ccccggactg ggggatagtt taatggtaga
     1621 acagcggact ctgactccgt tagtcgaggt tcgaatcctc gtcccccagc catttgatcg
     1681 tgctaagctt gcgcagctct cgttagaccc ggttggaccg gtttgtccgt gtcaaacttc
     1741 ctgtaactga agattcttgc cgaaccgggc cttgcagaat taggtttgcg cccgcacagg
     1801 gcacgaaccg agccaaatcc cgcaaagttt tcgcccgagg tgcgtgagcg caatgtttcg
     1861 gattgtgctg acggcgttgg gttagcttcg agcgtcggct cgcctgccgc cgtacggcgg
     1921 aggagacgga cgatgcgcat gggtgaatcg tcggaaaggt ctgtgctcca caactgaaga
     1981 cgaccatgga cagcatacgc attctcgaaa agctgattgc tttaccgacc gtgagccggg
     2041 actccaacct ggatctcatt gcgcacgtct cggatacgct gcgggatcac ggcatcgcca
     2101 gccgccttgt cccttctcag gatggtcaca aggccaatct tttcgcgacg atcgggccgc
     2161 aggatcgccc gggggttatg ctgtcggggc acacagacgt cgtgccagtg gatgggcaga
     2221 attggacgtt gccgcccttt gtgatgaccg agcgcgacgg caagctctac gggcgcggcg
     2281 cggcggacat gaagggtttt gttgcgtgcg cgctctccgc tggcatcaag gcctcgaaga
     2341 tcggcctggc cacgccactc catctggcct tctcttatga tgaggaggtt ggctgccttg
     2401 gcgtacgcag cttgatcgac cttctccagg cagcacccca taggccgttg ctgtgcatcg
     2461 tcggcgagcc gaccgggatg caggttgcca cgggccacaa gggcaagatc gccgcgcggg
     2521 cgctgtgcag ggggcgggaa ggtcattccg cgctggcgcc gctggcgctc aacgccatcc
     2581 atctgggttg cgacctcatc gccgtgctgc gcaaagagca ggatcggctg gcgcgcgagg
     2641 gcgcccgtga cggcgactac gacgttcctt acaccaccgt tcacgtcggc aggatcgacg
     2701 ctggcgtggc actcaacatc gtgccgaacc tttgccaggt cgatttcgag atccgcaatg
     2761 tgacggacga cgacccgcaa gcgatcctgg ctggcatccg cgcggaggcg gcgaggatcg
     2821 cggccgacgc atcatccctc gcccaggaag ccgcgatcga gatcgaggtg atcaacagtt
     2881 atcccggtct cgagacccct gccagctcgc aagctgtcgc cttcgtcaag tcgctgacag
     2941 gcgccaaccg cacgaccaag gtc
//
LOCUS       DASSGX010000110         6408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1545418, whole genome shotgun sequence.
ACCESSION   DASSGX010000110 DASSGX010000000
VERSION     DASSGX010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6408
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1545418"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="VFI12_01970"
     CDS             <1..733
                     /locus_tag="VFI12_01970"
                     /inference="COORDINATES: protein motif:HMM:NF028479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HET7055195.1"
                     /translation="KLVRTFGETGQKPGQFEFADFGAIGLDGDSDVYVLDTGNERVQK
                     FTPGLKFDLEWGKSGLEPGEFQHPSDIAVREDGMVFVVDALSGQVQQFDSSGTFVREI
                     VPTEIADQFYEPARLGLDAAGNLYVPDLTRIYVFDPNGTLSRTIQTNEVGNGEVYVAN
                     SAAVSESGFIYVSDWQSNRIAVFDPDGVFVGYLGSYGTGPGQFNEVDTLVTDDTGRLL
                     VLDFVNRRIQVFKLTEPPAGTPIAA"
     gene            complement(730..1905)
                     /locus_tag="VFI12_01975"
     CDS             complement(730..1905)
                     /locus_tag="VFI12_01975"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET7055196.1"
                     /translation="MKLSSAMSRLGTETAFEVLVRAKALEAKGKDVVHLEIGEPDFDT
                     PRNIIDAGCDALQSGWTHYGPSAGLPDLREAISTYLNRSRNTGYKAENVIVTPGGKPI
                     MFFVILALLEPGDEAIYPNPGFPIYESMINFTGAKAVPSPIRESNQFALDVDELEGLI
                     TPKTKLLIINSPGNPTSGVLPRVDIERIAELAVKHDLIVLSDEIYSEIIYEGEHVSIA
                     TMPGMTERTIVLDGFSKTYAMTGWRLGYGAMPVDFAGQINKLMVNSVSCTSVAVQRAG
                     IEALEGPQDDVRAMVAAFKARRDIMVEGLNAIPGVTCLNPKGAFYVFPNISGTGKSSR
                     EFADSLLDEHGVASLAGTSFGQHGEGYLRLSYANSEENLRKALDRIRCAAEASRGRN"
     gene            2324..2551
                     /locus_tag="VFI12_01980"
     CDS             2324..2551
                     /locus_tag="VFI12_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055197.1"
                     /translation="MMATKWHYYVRRGELSQDALNEIGKEGWELVSVDSSDDDPVFYF
                     KRPHPTLAEAITLEQRERYFDEWGVGQDQPS"
     gene            2548..2934
                     /gene="rbsD"
                     /locus_tag="VFI12_01985"
     CDS             2548..2934
                     /gene="rbsD"
                     /locus_tag="VFI12_01985"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: protein motif:HMM:NF008761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HET7055198.1"
                     /translation="MKKGGIYHPAINHLLASTGHTDYFTVCDRGFPVPIGPERIDLAL
                     TDGIPTVLDVVRAVMPEFHIDRVLIATEAMTASPKRVAELRALLDPIPLVTIDHLELK
                     QLSRAGRATIRTADTVPYANIIIVSG"
     gene            3007..4701
                     /locus_tag="VFI12_01990"
     CDS             3007..4701
                     /locus_tag="VFI12_01990"
                     /inference="COORDINATES: protein motif:HMM:NF015926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HET7055199.1"
                     /translation="MSDTEISIRVAGAAPGGRVIGVIDQDRKVHRQTPSFTRELLGTI
                     DAENIVRRDDGQEVGEPIAMIAGTRIVTPALDRATAPVLGFINDAGEVRPSGSDRVLG
                     VVDDANPLGMAFFALAYPRLVVEVDELEAEVRAAKNKLPFRDKIRKRAETLSDSDVLG
                     DLSALVARLGRLDNEIETEAGERTAAKERIATEAESLADSTDWKATGERMKALFEEWK
                     TIGSAGKEADEALWARFSSAREQFNTRRKTDFEERQATRGAAKERKEALIAQAEAAVA
                     GEQIDFRSVGDKLRSIQAKWKEAGSAGRADDDALWGRLQTITKPFYEQRSAWYTANQQ
                     AKEALIARAEEVSGSEDWNGTANALKALQAEWKQIGTAGREADETLWTKFRAANQAFF
                     GQREKVAADRNRNFSDNLSAKLELVKKAQQLRWAPDAYSAANEAKQLQAQWKEIGPAP
                     REKSDEIWKQFREACDKIFANVQSARNRQQSDWTDRLKEAMDRKLEQFGEILRSIDRD
                     QEQLDRLSQQLVAKPTPDLEKRVKEISGRIKDKEKRASSIEESLFEIRDKVHGGVD"
     gene            4810..5268
                     /locus_tag="VFI12_01995"
     CDS             4810..5268
                     /locus_tag="VFI12_01995"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HET7055200.1"
                     /translation="MTEVIPGADESFMRRCIDLAREARERGDAPVGALIVRDGLVIAE
                     AGERVDSVLDIAGHAEIVAIQTACRLLGTLNLSGCMLYSNAEPCVMCAYAIRQTGISR
                     VVFGATVPDMGGATSRYPILTAPDVSEWPEPPAVVAGVLVDECAALVSGR"
     gene            5372..>6408
                     /locus_tag="VFI12_02000"
     CDS             5372..>6408
                     /locus_tag="VFI12_02000"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET7055201.1"
                     /translation="MASESFAVAPDVLDRVLSAIDRRTDQIVRFASELIQQPSVNPDL
                     EPNAEAERPAQEWLRDQFAAMGSFPDIDFWEVAENRPNVVAVRTGEGGGRSLIWSAHT
                     DVVPVTPEQAEQWEGAGPFSGEVRDGWLWGRGASDMKGAIAAYVMAAQILHDEGVRLK
                     GDLRLAQSCGEEAGRRDIGCNTVLERGHRADMAIFPEPSNFAIYPTVKGELYFRLTVP
                     GKSTHICNRHLVAQPLPYGIERPGVSAIDNMLKYQLAILELEKQWNLWRTNEHVPAGG
                     MFININTMQAGTSLTSVPDSADATGSLLFNPDLTGEEVMAEIRATIDRVTQGDYWLRA
                     HPPVLDLPLDA"
BASE COUNT         1168 a         1868 c         2276 g         1096 t
ORIGIN      
        1 caaacttgtt cgcacgtttg gtgagacggg gcagaaaccg ggtcaattcg agtttgcgga
       61 cttcggggca attggtctcg atggggacag cgatgtctac gtcctcgata ccggtaacga
      121 gcgtgtccag aagttcacac cgggcctgaa attcgatctc gagtggggaa agtccggcct
      181 ggagccaggt gaattccagc acccctcgga tatcgccgtg cgcgaagacg ggatggtctt
      241 cgtcgttgat gctctttcag ggcaggttca gcagttcgat tcgtcgggca cattcgtgcg
      301 cgagatcgtt ccaacggaga tagcggacca gttctacgag cctgccaggt tgggcctgga
      361 tgcggccggc aacctctacg taccggacct cacgaggatc tacgtattcg accccaatgg
      421 aacgttgagc cgaaccatac aaaccaacga ggttggcaat ggtgaggttt acgttgcgaa
      481 cagcgccgcg gtctcggaat ccggcttcat ctacgtgtcg gactggcaat ccaatcgaat
      541 cgccgtcttc gatccggacg gcgtgtttgt tgggtatctg gggagttacg ggactggccc
      601 agggcagttc aacgaggtgg acacgctcgt cacggacgac acgggccggc tcctggtgct
      661 cgatttcgtc aaccggcgta tccaggtttt caagctcacc gaaccgcccg ccggcactcc
      721 gatagcagcc tagtttcggc cacgtgacgc ttcggcggcg cagcggatgc ggtcgagggc
      781 cttgcggagg ttttcctcgg agttggcgta actgaggcgc aggtagcctt cgccgtgctg
      841 gccgaatgag gtgccggcga gggaggcgac gccgtgctca tcgagcaggg aatcggcgaa
      901 ctcgcgactg gacttgccgg tgccgctgat gttcgggaag acgtagaagg cgcccttcgg
      961 gttcaggcag gtcacgccgg ggatggcgtt cagcccctcg accataatgt cccgccgggc
     1021 cttgaaggcc gcgaccatcg cccggacatc gtcctgcggg ccttcgagcg cctcgatgcc
     1081 ggcgcgctgg acggcgaccg aggtgcagga gacgctgttg accatcagct tgttgatctg
     1141 gccggcgaaa tcgaccggca tcgcgccgta gccgagccgc cagccggtca tcgcataggt
     1201 cttcgagaag ccgtcgagga cgatcgtgcg ctcggtcatg ccgggcatgg tcgcgatcga
     1261 gacgtgctcc ccctcgtaga tgatctcgct gtagatctcg tcggagagca cgatcaggtc
     1321 gtgcttgacc gccagctcgg cgatgcgctc gatgtcgacg cggggcagga cgccgctggt
     1381 cggattgccg gggctattga tgatcagcaa cttggtcttc ggggtgatca gcccctccaa
     1441 ctcatcgacg tcgagcgcaa actggttgga ctcgcggatc ggcgagggga cggctttcgc
     1501 gccggtgaag ttgatcatcg actcatagat cgggaagccc ggattggggt agatcgcctc
     1561 atcgcccggt tcgagcaggg cgaggatcac gaagaacatg atcggcttgc cacccggggt
     1621 gacgatgacg ttctcagcct tgtacccggt gtttctggaa cgattcagat aggtggagat
     1681 tgcctcgcgc aggtcgggaa ggccggcgga gggaccgtag tgggtccagc cggattgaag
     1741 ggcgtcgcag ccggcgtcga tgatgttccg cggggtgtcg aagtcgggct cgccgatttc
     1801 gaggtggacg acgtccttgc ccttggcttc gagggccttg gcacggacga ggacctcgaa
     1861 ggcggtttcg gtgccgaggc gggacattgc ggatgagagc ttcattgcct gctccagggt
     1921 gcgcgacatg gccgagttga catgcgcaag catacccggc gcggcgtcgg tagaatcggg
     1981 tgcgaggcgg gaaggcggga aggcgcggag gcgcgaagtt tggagatgtc agcggagttg
     2041 gagatgtagc aaggtgcggg aggcccgcgc ccggggttgg cgatgaacgg gttcgggatg
     2101 tcgaacgagc tgcccggcgc taaagcaccg ggctacatca gataagccag ctaaagcagg
     2161 ctggcggagg agcccgttta tggtgatagc cgttgacccg atcatcaatc cccggcgggg
     2221 ccgacgaccc ggtcgtttgt tgaagaacga tctcgctctg cgggcgattg cggggcacag
     2281 aggcacccgc gccacgattt ggcggaccag gggaggacac gcagtgatgg cgacgaagtg
     2341 gcattactac gttcggcggg gcgagttgtc gcaggatgcg ctgaacgaga tcggcaagga
     2401 gggctgggag ctggtctcgg tcgactccag cgatgacgat ccggtcttct acttcaaacg
     2461 gccgcacccg accctggccg aggcgatcac gctcgagcag cgggagcgct atttcgatga
     2521 atggggtgtc gggcaggacc agccgtcgtg aagaagggcg gcatctacca cccagcgatc
     2581 aatcacttgt tggccagcac cgggcacacc gattacttca ccgtttgcga tcgcggcttc
     2641 ccggtgccga tcgggccgga gcggattgac ctggcgctga cggacggcat accgaccgtg
     2701 ctggacgtag tccgcgcagt tatgccggag ttccacatcg accgcgtgct gatcgcgacc
     2761 gaggcgatga ccgcatcccc aaagcgggtg gccgagctgc gggccctgct cgacccgatc
     2821 ccgctggtga cgatcgatca cctcgagctg aagcagcttt cacgtgccgg ccgggcgacg
     2881 atccgaactg cggatacggt cccctacgcg aacataatca ttgtttcggg ctagaggcgg
     2941 gtacaatggc ggctgacagg tagccgtttg gctcgaatca ggtacgttct gcggagcaac
     3001 aggtcagtgt cggatacgga aatctcaatt cgcgtcgcgg gcgctgcgcc gggcggacgc
     3061 gtcatcggcg tcatcgatca ggatcgcaag gttcatcggc agacgccatc gttcacccgc
     3121 gaattgctcg gaacaatcga cgccgagaac atcgtgcgcc gcgatgatgg gcaagaggtc
     3181 ggcgagccga tcgcgatgat cgccgggacg cggatcgtga cacccgcgct ggatcgggcg
     3241 accgcaccgg tgctgggctt catcaacgat gccggcgagg tgcggccctc ggggtcggat
     3301 cgcgtgctgg gcgtggtcga tgatgcgaat ccgctcggta tggccttctt cgcccttgcc
     3361 tatcctcggc tggtggtcga ggtcgacgag ctggaggcgg aagtccgggc ggccaagaac
     3421 aagctcccgt tccgcgacaa gatccggaag cgggccgaga ccctgtccga ctccgacgtg
     3481 ctgggcgacc tgtcggcgct ggtggcgcgg ctgggccggc tcgacaacga gatcgagacc
     3541 gaggccggcg aacggacggc ggccaaggag cggatcgcga ccgaagcgga gtcgctggcc
     3601 gattcgaccg actggaaggc gaccggggag cggatgaagg cgctcttcga ggagtggaag
     3661 acgatcggca gtgccggcaa ggaggccgac gaggcgctct gggcccggtt ctcctcggcg
     3721 cgggagcagt tcaacacccg ccgcaagacc gatttcgagg agcggcaggc gacccgcggc
     3781 gcggccaagg agcgcaagga ggccctgatc gcgcaggccg aggcagccgt ggccggcgag
     3841 cagatcgatt ttcggtcggt cggcgacaag ctccgcagca tccaggccaa gtggaaagaa
     3901 gccggctcgg cgggccgggc cgacgacgat gcgctctggg gccggttgca gacgatcacc
     3961 aagccgttct acgagcagcg ctccgcctgg tacaccgcga accagcaggc caaggaggcg
     4021 ctgatcgcgc gggccgagga ggtaagcggc tccgaagact ggaacgggac ggcaaacgcc
     4081 ctcaaggcgc tccaggccga gtggaagcag atcggcacag ccggtcgcga ggcggacgag
     4141 acgctctgga cgaaattccg ggcggcgaac caggccttct tcggccagcg cgagaaggtc
     4201 gcggccgatc gcaaccgcaa tttctcggac aacctgtcgg cgaagctgga gctggtcaag
     4261 aaggcgcagc agctgcgctg ggcgccggac gcgtacagcg ccgcgaacga ggccaagcag
     4321 cttcaggcgc agtggaagga gatcggcccg gctccacgag agaagtcgga cgagatctgg
     4381 aagcagttcc gggaggcgtg cgacaagatc ttcgcgaatg tccagagcgc gcggaaccgg
     4441 cagcaaagcg attggaccga ccggctcaag gaggcgatgg atcgcaagct ggagcagttc
     4501 ggcgagattc tgcgctcgat cgaccgggat caggagcagc tcgaccggct gagccagcag
     4561 ctcgtggcca agccgacacc cgacctcgag aagcgcgtca aggagatctc cggccggatc
     4621 aaggacaagg agaagcgggc ctcgagcatc gaggagtcgc tcttcgagat ccgcgacaag
     4681 gtccacggcg gcgtggacta gctccgctct ccgggacgct cgcccgggag aagccgactg
     4741 accaacgctg cattcgatat ctggcgccgg tccctcgtgg ccggcgctct gttcatggct
     4801 ggggaagcga tgactgaggt gattccaggc gctgacgagt cgttcatgcg gcgctgcata
     4861 gacctcgccc gcgaggcccg ggaacgcgga gatgccccgg tcggagccct gattgtccgc
     4921 gacgggctgg tcattgccga ggccggtgag cgggttgatt cagtattgga catcgcggga
     4981 cacgcagaga tagtcgcgat tcagacagcc tgccgattgc tgggcacgct caacctctcc
     5041 ggctgcatgc tctactccaa tgctgagccg tgcgtgatgt gcgcatatgc gatccgccag
     5101 acggggatca gccgtgtcgt ttttggcgcg accgtccccg acatgggtgg cgcaacctcc
     5161 cggtatccaa tccttaccgc acctgatgta tcggagtggc cagaaccgcc ggcggtcgtc
     5221 gcgggcgtgc ttgtggacga gtgcgccgcg cttgtttctg gccgctgacg gtcaattacc
     5281 cggtttcctc tcggctacca gttccccgct tcctgcccgt tcggtacact gccgacaatc
     5341 tggcttgcct catctccatg gagactgcgt aatggcgtcc gagtcgtttg ctgttgcccc
     5401 cgatgtgctg gaccgcgtgc tgagcgcgat cgaccggcgg accgatcaga tcgtgcggtt
     5461 cgcgtctgaa ttgattcagc agccgtcagt caaccccgat ctggagccga acgcggaggc
     5521 cgagcggccg gcacaggagt ggctgcggga ccagttcgcg gcgatgggtt cgttcccgga
     5581 catcgatttc tgggaggtgg ccgagaaccg gccgaacgtg gtcgcggtcc ggacgggcga
     5641 gggcggcgga aggtcgctga tctggtcggc gcatacggac gtcgtgccgg tgacgccgga
     5701 acaggcggaa cagtgggagg gcgcggggcc gttctcgggc gaggtgcggg atggctggct
     5761 ctgggggcgg ggcgcgtcgg acatgaaggg cgcgatcgcg gcctacgtga tggcggcgca
     5821 aatcctgcat gacgagggcg tgcggctgaa gggcgacctg cggctggcgc agtcgtgcgg
     5881 cgaggaagcg gggcggcgcg acatcggttg caatacggtg ctggagcgtg gtcaccgggc
     5941 ggacatggcg atatttccgg agccatccaa cttcgcgatc tacccgacgg tcaagggcga
     6001 gctctatttc cggctgaccg tgccgggcaa gtcgacccat atctgcaacc ggcacctggt
     6061 cgcccagccg ttgccgtacg ggatcgagcg gccaggggtg agcgcgatcg acaacatgct
     6121 caagtaccag ctcgcgattc tcgagctcga gaagcagtgg aatctctggc gcacgaacga
     6181 gcatgtgccg gcgggcggga tgttcatcaa catcaacacg atgcaggccg ggacctcgct
     6241 gacctcggtg ccggattcgg ccgatgcgac cggatcgctc ctcttcaacc cggatttgac
     6301 cggcgaggag gtgatggcgg agatccgggc gacgattgat cgggtcacgc agggcgatta
     6361 ctggctgcgg gcgcatccac ccgtgctgga tctgccgctc gatgcgga
//
LOCUS       DASSGX010000111         3167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_129892, whole genome shotgun sequence.
ACCESSION   DASSGX010000111 DASSGX010000000
VERSION     DASSGX010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129892"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1185
                     /locus_tag="VFI12_02005"
     CDS             <1..1185
                     /locus_tag="VFI12_02005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HET7055202.1"
                     /translation="PSVTGNEHDVQQAIARQMNDLALDIDMWEPDAAALAPYAEHVGE
                     FESFAGRPNVVGTRRGRGGGRSMILNAHIDTVEAGDPAQWSVDPFLGALRAGRIYGRG
                     ACDMKAGLVGELMALRALDELGVTLNGDVFVESVISEEDGGAGALATILRGYTADAAI
                     ITEPTNLAIIAAQGGSLVFRLTITGRSAHACVRDEGVSALEKFLPIHAAILAHERAHH
                     EAIDHPLYQRFGNRAPINIGTVRAGNWPSSVPESIVVEGRAGLVPGETLEETKAALLA
                     TIEEAVAGDPWFAAHPPQIEWFSGQFTAAETSTDDPLTHALQEAHAGVTGQRAVIDAA
                     TYGADMRHFVNIGRIPCLMYGAGDVALAHRPDEFVPISEVKTVAKTLAVTLARWCGVN
                     HI"
     gene            1441..2280
                     /locus_tag="VFI12_02010"
     CDS             1441..2280
                     /locus_tag="VFI12_02010"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET7055203.1"
                     /translation="MKFAAHEGVGARTLAYTNLPSARRNGVYAAPGWFAEPNGQNGSV
                     AITTHTTNGNGHADANRPAHTGASTVTYTRGQEIFTPGQGQGLVYIVRSGCVRLYKVL
                     ADGRSINLGLLGPNTVFTQEDTSDGIASGSVAEAMVDSTLAIVDTDSLASIIADSPEL
                     AAAMVTGMSRRLTELQTLVEHLLVRDTAVRLATTLLSLANRFGRPTADGMIAIALPVT
                     HQSLAGMIGSNRVTVTRKLLELQEGGYVRSLGRNAIAVNPPKLREYAQVAREHGDELD
                     GDW"
     gene            2363..>3167
                     /locus_tag="VFI12_02015"
     CDS             2363..>3167
                     /locus_tag="VFI12_02015"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HET7055204.1"
                     /translation="MARILITGGTGFVGSNIRKLIPDRELRLLVRDSSSARDTGAAEL
                     VRGDVLDPVSLDQAMTGVDAVIHLVAIIEESGDQTFDRVIRQGTEHVVSAAKRAGVRR
                     FIHMSALGARSDPAFPYLNAKWRAEEAVRDSGLEWTIIRPSVIFGPGDGFVTILANLV
                     RKAPVIPVVGAGQSKFQPIAVAEVAEAYRRALDDPGTIGQTYELGGGEIYSYESMLDV
                     IAAHLGKGKPKIHLPVGLMKAIVAVSAPLPKALRPPVTKEQLNMLAIDNC"
BASE COUNT          576 a         1049 c         1002 g          540 t
ORIGIN      
        1 ccgagcgtga ccggcaacga gcacgacgtc cagcaagcca tcgcccgcca gatgaacgat
       61 ctggcgctgg acatcgacat gtgggagccc gatgccgccg cgctcgcccc gtacgcggag
      121 cacgtcggcg agttcgagtc atttgccggc cgcccgaacg tcgttggcac gcggcgcgga
      181 cgtggtggtg gccgctcgat gatcctcaac gcccacatcg acacggtcga agcgggcgat
      241 ccggcccagt ggtcggtcga tccctttctc ggcgcgctcc gggccggccg gatttacggg
      301 cgtggcgctt gcgacatgaa ggccgggctg gtcggtgagt tgatggcgct gcgggcgctc
      361 gatgagctcg gcgtcacgct caatggcgac gtcttcgtcg aaagcgtgat ctccgaagag
      421 gacggcggcg cgggggcgct cgcgacgatc ctgcgcggct acaccgccga tgccgcgatc
      481 atcaccgagc cgacgaacct ggcgatcatc gccgcccagg gtggatcgct cgtcttccgc
      541 ctgacaataa ccggccgctc ggcgcatgcc tgcgtccggg acgagggggt cagcgcgctc
      601 gagaagttcc tgccgatcca cgcggcgatc ctcgctcacg agcgagcgca tcacgaggcg
      661 atcgaccacc cgctctacca gcgattcggc aatcgcgcac cgatcaacat cggcacggtc
      721 cgtgccggca actggcccag ctcggtgccg gaatcgatcg tcgtcgaggg ccgagcgggc
      781 ctcgtgccgg gcgagaccct cgaggagacg aaggcggcgc tgctggcgac catcgaagag
      841 gcggtcgcgg gcgatccctg gtttgccgcg catccgccgc agatcgaatg gttcagtggc
      901 cagttcaccg cggccgaaac gtccacggac gaccccctga cgcacgcgct acaggaggcc
      961 catgccggcg tgactggcca gcgcgcggtc atcgatgccg caacgtatgg ggccgatatg
     1021 cgccatttcg tcaatatcgg ccggattccg tgcctgatgt acggggcggg agatgtcgct
     1081 ctggcccacc ggccggacga atttgtgccg atttccgagg tgaagacggt cgccaaaacg
     1141 ctcgccgtca ccttggcgcg ctggtgtggc gtgaatcaca tctgatttcg cttgtcgcgc
     1201 aatgctgtgc aaaagctcgg gtgaacgtac aagcaaaacc tgtgcaaggg tgggcatgcc
     1261 ctttgcacac gcgctttttg ggcgtaatcg cacaagactt ctgtgcaaag tattgacatt
     1321 cgctgggtag ccccctacac tcgacctatc gaagttcagg ctacggtgtg cagcggcgca
     1381 cactacccgc ggtcaaaacg ccgcgggaga gcctggacgg tctgaaggag ggaacccgtg
     1441 atgaagtttg cagcccatga aggcgttggc gctcgcacgc tcgcgtacac caatctgcca
     1501 tcggcgcgac ggaatggggt ttacgcggcg cccggttggt tcgccgaacc gaacggtcaa
     1561 aacggatcag tagcaattac tacgcacacc accaacggca acggacatgc cgatgccaac
     1621 cgcccggccc acaccggcgc cagcacagtc acctacaccc gcggccagga aatcttcacg
     1681 ccgggccagg gccaagggct cgtctacatc gtccgctccg gttgtgtccg cctctataag
     1741 gtgcttgccg acggccgctc gatcaacctc ggtctgctcg gacccaacac cgtcttcacg
     1801 caggaggaca cctccgacgg gatcgcatcc ggttctgtcg ctgaggcgat ggtggactcg
     1861 acgctggcca tcgtcgacac ggactcactc gcgtcgatca tcgccgactc cccggagctc
     1921 gccgccgcga tggtcacggg tatgagccgc cgcctcaccg aactgcagac gctcgtcgag
     1981 cacttgctgg tccgcgacac cgccgttcgt ctcgcgacca cgctcctgtc gctcgccaat
     2041 cgtttcggcc gaccgactgc cgatggcatg attgctatcg ccctgccggt tacgcaccag
     2101 tcgctggccg gcatgatcgg ctccaaccgc gtcacggtca cccgcaagct gcttgagctc
     2161 caggagggtg gatacgtccg ctcgctcggc cgcaatgcta tcgccgtcaa tccgccgaag
     2221 ctgcgagagt acgcgcaggt cgcccgcgag cacggcgacg agctcgacgg ggactggtag
     2281 agtcggctgc gcccccgcca ctccaatccg ggtaggcttt cctttataag gaaactgaac
     2341 cagaacggaa gggagaagcg gcatggcgcg gatactgatc accggcggga ccggcttcgt
     2401 cggctccaac attcggaagc tcataccgga tcgcgaactg aggctcctcg ttcgcgattc
     2461 gtcgtctgcg cgggacaccg gcgccgcgga gcttgtgaga ggcgacgtgc tcgatccggt
     2521 ttcgctcgac caagccatga ccggcgtcga tgcggtcatc cacctggtcg cgatcatcga
     2581 agagtcgggc gaccagacct tcgatcgggt cattcgccag ggaaccgaac atgtcgtctc
     2641 ggcggccaaa cgcgcgggag ttcgccgctt cattcacatg agcgcgctcg gcgcccggtc
     2701 tgaccctgcc ttcccgtatc tcaatgcaaa atggcgcgcg gaagaggcgg ttcgcgactc
     2761 cggtctcgaa tggaccatca tcaggccgtc ggtcatcttc gggcccggcg acggcttcgt
     2821 cacaatcctt gcgaacctgg tccgcaaggc gccggtcatt cccgtcgtcg gcgctgggca
     2881 gagcaagttc cagccgatcg cagtcgcgga ggttgccgaa gcctaccggc gagcactcga
     2941 tgatcccggt acgatcgggc agacgtatga gctgggcggc ggcgagattt acagctacga
     3001 gtcgatgctc gacgtcattg ctgctcacct cggcaaaggc aaaccgaaaa tacacctgcc
     3061 agtcggattg atgaaggcga tcgtcgccgt gtctgcgccg ctcccgaaag cgctccggcc
     3121 gccggtcact aaagagcagc tcaacatgct cgcgatcgac aattgca
//
LOCUS       DASSGX010000112         3325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_21439, whole genome shotgun sequence.
ACCESSION   DASSGX010000112 DASSGX010000000
VERSION     DASSGX010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21439"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="VFI12_02020"
     CDS             complement(<1..499)
                     /locus_tag="VFI12_02020"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7055205.1"
                     /translation="MRKRVLIIEDDAGIARLLLDNLTIDGFDVRCAHDAHDGLVACQE
                     FLPDLVLLDVMLPDRIGFDLVGLLRQGGRRSVIVLSARGSKADKVDGLTRGADDYVTK
                     PFDIEELVARIHAVLRHTQPAANVARIGRIEFDFEQHQATGPSGQVHFTAREFEILRY
                     LAAHHH"
     gene            complement(539..1861)
                     /locus_tag="VFI12_02025"
     CDS             complement(539..1861)
                     /locus_tag="VFI12_02025"
                     /inference="COORDINATES: protein motif:HMM:NF011656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7055206.1"
                     /translation="MSTHPNKRGTIRNVVIVSSGNFLEMYDFMVFGYYASAIGHAFFP
                     ASSEFASLMLSLATFGAGFLMRPLGALVLGAYVDRHGRRAGLLLTLALMAIGTVSIAC
                     VPGYATIGLAAPVIVLVGRLMQGLSAGAQIGGVSVYLAEIATPGRRGFYVSWQSASQQ
                     VAVTFAALVGLAATTLLTPPQMLAWGWRIPIGLGCLLIPFLFLIRSYMEESEEFLAQA
                     ERPTASAVFRSIAINWRVVVLGTLLATMTTVSFYLITAYTPTFGSTVLHLKPSDAMAV
                     TLCVGVCNFVLLPTMGALSDKIGRRPLLIGCTLVALATAYPSLSWLVADPSFARLLIV
                     EIWLAIVYATYNAAMIVHLTEVMPPRVRATAFSLAYSTATAIFGGFTPAICTYLIHVT
                     GNRATPGLWLSAAALCGLTAAIALGADRKVLQAHHSPTGGAVLKQAAK"
     gene            complement(1888..>3325)
                     /locus_tag="VFI12_02030"
     CDS             complement(1888..>3325)
                     /locus_tag="VFI12_02030"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HET7055207.1"
                     /translation="GGNLATKEVADDFAYMGIDLVSRANNKQTDYASVWEDFQQVERV
                     GIIHAGVARTLPEARMPRNFATPKGLVGFVGLTSDGGTDACCAGGQVVSVTAEQLAQV
                     KAMKDALLARRDEVEIPVELPAPDPEGTVNVFGVTFRQGANSSGAQAAETTGRGGRGG
                     GAGRNGPGNWVKNTVKLTLFHGVTAEQMKQLRAIAGDSGTGTDLHAFGTQFRLMDRPG
                     EHSFDMDPQDLKEMLTQIRSGKQGADFLATNFHWHQNRYDFQAYSHDHFPADFEIKFA
                     HMAIDQGDDMFAAQGVHTIKGVEIYKGKPIFYGLSNFIFQAGIMARAKGSQLFVPPGS
                     PVAADQSAAAPQGRAGGRGAGDADQPAAGRGRGGRGGRGAPAPAGDEIVGEYQYGGSW
                     QNLANMESLVGESHYENGQLTEVRVYPVDLGQTPRPMSQLGIPRRPTPAVATKILNEL
                     IEYSKPFNTKIVIENGVAVIRIPPSERH"
BASE COUNT          614 a         1091 c         1048 g          572 t
ORIGIN      
        1 gatgatgatg tgccgccaga taccgcagaa tttcgaactc gcgcgccgtg aagtgtactt
       61 gccctgaagg ccccgtggcc tggtgttgtt caaaatcaaa ctcgatgcga ccgatgcgag
      121 cgacgttcgc ggccggttgc gtgtgcctga ggaccgcgtg gatgcgcgcc accagttctt
      181 cgatgtcgaa gggcttggtg acgtagtcat ccgcgccgcg cgtgagccca tcgactttgt
      241 ccgctttgct gccgcgtgcc gacaacacaa tcacgctgcg tcgaccgccc tggcgcagca
      301 agcccaccaa atcgaatcca atgcgatccg gcagcatgac atccagaaga accaggtcgg
      361 gcaggaattc ctgacacgcg acgagcccgt cgtgggcgtc atgcgcgcat cgcacgtcga
      421 agccgtcgat cgtgaggttg tccagcagca accgggcgat gcccgcgtcg tcttcgatga
      481 tcagaacgcg tttgcgcaca ggtagagaaa gccccggaca atcgtgctcg agctcaggct
      541 attttgccgc ctgcttcagg acggccccgc cagtcggcga atggtgtgcc tgcaggacct
      601 ttcgatccgc tccgagagcg attgcagcgg tcaagccgca caaggctgct gcagacaacc
      661 acagtcccgg cgtcgcccga ttgccggtca cgtggattag atacgtacaa atggcgggcg
      721 taaagccgcc aaaaatggcg gtcgcggtac tgtaggccag agagaacgcc gtcgctcgga
      781 ctctgggcgg catcacctcg gtgaggtgga cgatcatcgc ggcgttatac gtggcgtaaa
      841 cgatggcgag ccagatttcg acgatcagaa gccgggcaaa ggacggatcc gccacgagcc
      901 acgacagtga cgggtaggcc gtcgcgagcg cgacaagggt gcagccgatc aagagcggtc
      961 gacgaccgat cttgtcggac agtgctccca tggtcgggag cagcacgaag ttgcagacac
     1021 caacgcagag cgtcaccgcc atggcatcac tcggcttcaa gtgcagcacg gtgctgccga
     1081 aggtcggcgt gtacgcggtg atgagataga acgagacggt cgtcatcgtc gccagcagcg
     1141 tcccaaggac gacgactcgc cagttgatgg caatcgagcg gaagacggcc gatgcggttg
     1201 ggcgctcagc ctgcgcgaga aactcctcgc tctcctccat atacgagcga atcaagaaca
     1261 gaaacgggat gagtagacaa ccgaggccga tcggtatccg ccagccccac gccagcatct
     1321 gcggtggggt cagcagcgtc gtcgccgcca atcccaccag agccgcgaac gtgaccgcca
     1381 cctgctggct ggccgattgc cagctcacgt aaaaccctcg ccggcctggc gtcgcaatct
     1441 ctgccaggta caccgagacg ccgccgatct gcgcgcccgc ggagagcccc tgcatgaggc
     1501 ggccgaccag cacgatgacc ggagcagcca ggccgatcgt cgcgtagccc ggcacgcagg
     1561 cgatgctcac ggtcccgatc gccatcaggg cgagcgtcaa caacaggccc gccctccggc
     1621 cgtgtcggtc gacatacgcg cccagcacga gcgcccccaa gggccgcatc aggaatccgg
     1681 ccccgaaggt cgccagcgag agcatcagcg acgcgaactc gctgctggcc ggaaaaaacg
     1741 cgtgccctat cgcagaggcg tagtaaccaa acaccatgaa gtcatacatt tccaggaaat
     1801 taccgctgga gacgatgacg acattgcgaa tcgtgccgcg cttgttcgga tgagtgctca
     1861 ccgtacgctc acttgctcgt ccggccgcta gtgacgctcg ctcggcggaa tgcgaatcac
     1921 cgccacgcca ttctcgatga cgatcttcgt gttgaacggc ttcgaatact cgatgagctc
     1981 gttgagaatc ttcgtggcca ccgcgggcgt gggacgccgc gggatcccaa gttgcgacat
     2041 gggacgcggc gtctgaccga gatccacggg gtacacccgc acttcggtca gctgaccgtt
     2101 ctcgtagtgg ctctcgccaa ccagcgactc catgttcgcg aggttttgcc atgagccgcc
     2161 gtactggtat tcgccgacaa tttcatctcc agccggcgcg ggcgctccgc ggccgccgcg
     2221 tcctccgcga ccacgaccgg ccgccggctg atccgcatcg cctgcgccgc gtccacccgc
     2281 ccggccttga ggcgccgcgg cactttgatc cgcggcaacc ggcgatccag ggggcacgaa
     2341 cagctgggac cccttcgcgc gcgccatgat gccggcctgg aaaatgaagt tggacaagcc
     2401 gtagaagatc ggcttgccct tgtagatctc gacccccttg atcgtgtgca cgccctgggc
     2461 cgcgaacatg tcgtcgccct ggtcgatggc catgtgcgcg aacttgattt cgaaatcggc
     2521 cggaaagtga tcgtgggagt acgcctgaaa gtcgtagcgg ttctggtgcc agtggaaatt
     2581 cgtcgcgagg aagtcggcgc cctgcttgcc gctgcggatc tgcgtcagca tctccttcag
     2641 atcctgcggg tccatgtcga agctatgctc gcccggccga tccatcaggc gaaactgcgt
     2701 gccgaaggca tgcaggtcgg tgcccgtgcc ggagtctccc gcgatcgcgc gcagctgctt
     2761 catctgctcc gcggtcaccc cgtggaagag cgtgagcttg acggtgttct tgacccagtt
     2821 acccggcccg ttccggccgg cgccgccgcc ccggccgccg cggccggtgg tttcggccgc
     2881 ctgggcacct gaggagttcg cgccctgccg gaaggtcacg ccgaagacat tgaccgtccc
     2941 ttcgggatcg ggcgccggga gctctaccgg gatctccacc tcgtctcgac gcgccaggag
     3001 cgcgtccttc atcgctttca cctgcgcgag ctgctcggcg gtgacgctca ccacctggcc
     3061 gccggcgcag caggcgtcgg tcccgccgtc gctggtgagt ccgacgaatc caacgagccc
     3121 cttgggcgtg gcgaagttcc gaggcattcg cgcttcggga agcgtgcgcg ccacgccggc
     3181 gtgaatgatc ccgacgcgct ccacctgctg gaagtcctcc cacacgctgg cgtagtcggt
     3241 ctgcttgttg ttggcgcggc tgaccagatc gatgcccatg taggcgaagt cgtcggccac
     3301 ttctttggtg gccaggttgc cgccg
//
LOCUS       DASSGX010000113         4164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_456424, whole genome shotgun sequence.
ACCESSION   DASSGX010000113 DASSGX010000000
VERSION     DASSGX010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4164
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456424"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1698)
                     /locus_tag="VFI12_02035"
     CDS             complement(<1..1698)
                     /locus_tag="VFI12_02035"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HET7055208.1"
                     /translation="MNITTWPAHGLSPHRQAAGTTRSPGQPWLRSISLLIIVGLLSWI
                     GLAQLRTPDVIPATAPATSFSAERAMTHLDVIASESRAIGLPGHDATRDYLVTQLTAM
                     GLHPEIQTTTSVLRFEGADAFGAGTISNVIARIPGTANTGAIAINAHYDGGATGPAAG
                     DNGVGVAVTLETVRASLAGRPRATDLIIVFSDAEEIGDLGAAAFNQQHPWARDVRIAI
                     NFEAQGTGGPAMLYATSEHDGWVTGGYLSVAPEPSAYSMLPELVRAMPGMRLACDLED
                     YLLNGAAGLGFVLADGTARYHAATDTVANIDRGSVQQEGGNTLAAVRHFGSADLATVP
                     TAPTRVYFTILPGVVVHYGGDWAIGFAVLASVLAATAIVLGYRRRLVGVGGTAIGVAA
                     LIAGTIITALLVDLIWFLVKATNADYRVLLVGPYQSHQIVLALTLLAVAIMAAFYTLL
                     QKRIAALNLMAGAMLMWTLLLWLVTLAAPGASYYLLWPLLFATIAFGWLVLTHDRNRH
                     ATVDLAVLAVALVPVVLLVPGVTYQTVPLLHRVEYLMAITGGIPLLGLWAIFVAPLVG
                     "
     gene            complement(2052..2615)
                     /locus_tag="VFI12_02040"
     CDS             complement(2052..2615)
                     /locus_tag="VFI12_02040"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HET7055209.1"
                     /translation="MERNPEPQILDRRTALRMGGVAGVTVAAGALSTRQAAAQTPAAG
                     AGAMSVSRQSIALAAAQSLIEAAQAKAAEISVPMAVAIVDDGGLLKAFARMDGLDRAV
                     SVDLVQMKAYTAASFRSPTHMIAEGAKDDPVLAATLAAIPRFTLLGGGYPIKDSDAVV
                     GGIGCGGGTPEQDMQVAEAALSAVGGM"
     gene            2778..3842
                     /locus_tag="VFI12_02045"
     CDS             2778..3842
                     /locus_tag="VFI12_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055210.1"
                     /translation="MTGTALLAAGIGTAVSEFPARAEDLPDPTLVLTDSDSDTVTFLD
                     LATDERESVQVGIAPWGIARYGWRAFTATAEGVAVLDLRQRERTALIPYETRPESLGY
                     GEYRPGGMGIAVSPDGERVFVGNYLANGSSRLEVIDWQAELVLGSVGSGVRPFDVLAS
                     RDGRAAYSIDHDSYTVTVLDLESMSGRILPVSPLGDALGLAGFEKPHYAVLYRDGRLL
                     LPFQGQVLARLDPASGEYESIPLIANTHQHGIAINAAGDRLLIVGTGPAGGATSGASL
                     TVMDLVTNEQSLTPLTRPHERVAFSPDERLAYLTGGYSFAGQGWDGITIVDLVRQTTR
                     EIAVGSKPLDLLVLANAENS"
     gene            3884..>4164
                     /locus_tag="VFI12_02050"
     CDS             3884..>4164
                     /locus_tag="VFI12_02050"
                     /inference="COORDINATES: protein motif:HMM:NF012576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET7055211.1"
                     /translation="METRRTKPAGTRRVTIKDVAAEVGVAVSTISNAYNRPDQLSATL
                     RQRIFDTAEKIGYAGPNPVARGLRRGTSSLIAVLLGQPLAAAYDDPVTAA"
BASE COUNT          712 a         1446 c         1340 g          666 t
ORIGIN      
        1 gccgaccagc ggcgcgacaa agatcgccca cagcccgagc agcggaatcc caccggtaat
       61 ggccatcaag tactcgaccc ggtgcaggag tggaaccgtc tgatacgtca cgcctgggac
      121 cagcaggacg accggaacga gcgccacggc caagacggcc aagtcgaccg tcgcgtggcg
      181 attgcggtca tgtgtcagca cgagccagcc gaatgcgatc gtcgcgaaga ggagcggcca
      241 aagcaggtag tagctcgcgc caggtgccgc cagggtcacc aaccagagca ggagtgtcca
      301 catcagcatc gcgccggcca tcaggttcag ggccgcgatc cgtttctgga gcagggtata
      361 gaaggcggcc atgattgcaa ccgccagcaa tgtcagtgcc agcacgatct ggtgcgattg
      421 gtagggccca accaggagca cccggtaatc cgcattggtc gccttgacca gaaaccagat
      481 caggtccaca agcagtgccg tgatgatcgt gccggcaatt agcgccgcca cgccgatcgc
      541 ggttccaccc acgccaacca atcgccggcg atagccgagg acgattgccg tggcggccag
      601 gactgacgcg agcaccgcga atccgatcgc ccaatcgccg ccgtagtgca cgacaacgcc
      661 gggcagaatc gtgaagtaga cccgcgtcgg agcggtcggc accgtcgcca ggtcggccga
      721 accgaagtga cgcaccgctg ccagcgtatt gccgccctcc tgctgcacgc tgccacgatc
      781 gatgttcgcg acggtgtccg ttgccgcgtg gtagcgcgct gttccatcgg cgagcacgaa
      841 gcccagcccg gccgcgccgt tgagcaggta gtcctcgagg tcgcaggcca gccgcatgcc
      901 gggcatcgcc cggaccagct ccggcagcat cgagtaggcg ctcggctcgg gcgcgaccga
      961 gaggtatcca ccggtgaccc agccgtcgtg ctcgctcgtt gcgtagagca tcgccgggcc
     1021 gccggtgccc tgggcttcga aattgatcgc gatccgtacg tccctggccc atgggtgctg
     1081 ctggttgaag gcggccgcgc cgaggtcgcc gatctcctcg gcatcgctga agacgatgat
     1141 caggtcggtc gcccgcggcc ggccggccag acttgcccgg acagtctcga gcgtcactgc
     1201 cacgccgacg ccgttgtcac ccgcagccgg acctgtcgcg ccaccgtcat agtgggcgtt
     1261 gatcgcgatt gcgccggtgt ttgccgttcc cgggatccgt gcgatcacat tcgagatcgt
     1321 ccccgctccg aacgcgtccg caccctcgaa ccgcaggacc gacgtcgtcg tctggatctc
     1381 cggatgcagc cccatcgccg tcaattgcgt gaccaggtaa tcccgggtcg cgtcatggcc
     1441 gggcagaccg attgcgcggc tctcgctggc gatgacatcc aggtgcgtca tcgcgcgctc
     1501 ggccgagaac gacgtcgccg gtgcggtggc cgggatgacg tcgggcgttc gcagctgcgc
     1561 caggccgatc caggagagca gcccgacgat gatcagcaaa gagatgcttc gcagccacgg
     1621 ttgccccggc gaacgcgtgg tcccggccgc ctgtctatgg ggagagaggc catgtgccgg
     1681 ccaagtggtg atgttcatcg atctcctccg aacgcgcccg gagctgtggg gcatcccggg
     1741 tctctgcaac gcgcggaagg tccggcgccg gcgtatcgcg atggaggtga acggtgggaa
     1801 gagaggtgtg atccgggtgg atccgcgacc cgccgcgccg gccttccgta gcgcgggacg
     1861 ctcgccgggc gaccatcccg cgcctgcatg gtcaggctat tggccggatt gaaacatccg
     1921 tgaccgacat tagaacgccg acattacaaa atcgagacac gcacccgccc aatccgcgca
     1981 aagcggcagg caagaacaaa cccgctcacg tggagcgggc ttgttccgag gagggggagg
     2041 ggaaatgcgc gttacatacc accgacggct gagagcgcgg cttcggctac ctgcatgtcc
     2101 tgctcgggtg tgccgccgcc gcatccgatg ccgcccacga ccgcatcgga atccttgatc
     2161 ggatagccgc cgccaagcag cgtgaagcgc ggtatagccg ccaacgtcgc cgccaggacc
     2221 ggatcgtcct tcgcgccttc ggcgatcatg tgggttggag atcgaaacga tgcggccgtg
     2281 tacgctttca tttggaccag atcgacgctc acggcgcgat cgagaccgtc catccgggcg
     2341 aacgctttga gcaggccacc gtcatcaacg atcgcgaccg ccatcggcac gctgatctcc
     2401 gccgccttgg cctgcgcagc ttcgatcaac gattgcgcgg ctgccagcgc gattgactgc
     2461 cgcgagacgc tcatcgcgcc ggctccggcc gccggtgtct gggcggcggc ctgccgcgtg
     2521 ctcaaggccc ccgcggcgac tgtcaccccg gcaacgccgc ccattcgcag cgcggtccgg
     2581 cgatcgagta tctggggttc gggattccgt tccatgactc tccccttcgt ggaccgttcc
     2641 ctgcccggca atggcaggta tgccgcctct acgggctaga atcgcaatcg gattgccgca
     2701 cgcgtggcgc acgctccccg cgctgttcgc aagctggagg ccatatgcca ccctgcaccc
     2761 gccggaggat gctagagatg accggaaccg cgctcctcgc tgccgggatc ggcacggctg
     2821 tctcggaatt tcccgctcgc gccgaggacc taccggaccc aaccctcgtc ctgaccgatt
     2881 ccgacagcga tacggtgacc ttcctcgatc tggcaacgga cgaacgggaa tcggttcagg
     2941 tcgggatcgc gccgtggggc atcgcccggt acggctggcg cgccttcact gccaccgccg
     3001 agggcgtcgc cgtcctcgat ctccgccagc gggaacggac cgccctgatc ccgtatgaga
     3061 cccgaccgga gtcgctcggg tacggcgagt accgaccggg cggcatgggt atcgcggtct
     3121 cgccggacgg cgaacgcgtc ttcgtcggaa actacctggc gaatgggtcg agccggctcg
     3181 aggtgatcga ctggcaagcg gaattagtgc tcggctcggt cggatccggc gtccgtccgt
     3241 tcgatgtcct cgcgagccgc gacggccgcg ccgcctactc gatcgaccac gactcctaca
     3301 ccgtgacggt cctcgacctg gaatcgatga gcggccgcat cctgccggtc tcgccgctcg
     3361 gcgatgccct cggcctggcc ggcttcgaga agccgcatta cgccgtgctc tatcgcgacg
     3421 gtcgcctgct cctgccgttt cagggccagg tcctcgcccg gcttgacccg gcctccggcg
     3481 agtacgagtc aatcccgctg atcgccaaca cgcaccagca cggcatcgca atcaacgccg
     3541 ccggcgatcg cctgctgatc gtcggcaccg ggccggccgg cggcgcgacc agcggcgcat
     3601 ccctgaccgt gatggacctc gtgacgaatg agcaatcgct gaccccgctc acccgaccgc
     3661 atgagcgcgt cgccttcagc ccggacgagc ggttggccta cctgaccgga ggctacagct
     3721 tcgcggggca gggctgggac ggcattacga tcgtcgacct tgtacggcag accacccgcg
     3781 agatcgctgt cggctccaaa ccgctcgatc tcctggtgct cgccaacgca gagaattcct
     3841 gatattgcct gcgtcttcgg ggcaggtgtg gtagtttgcc cctatggaaa cacgacgcac
     3901 gaaaccggct ggcacccgtc gcgtcacgat caaggatgtt gcggccgagg tcggcgtagc
     3961 cgtctcgacc atctccaacg cctacaaccg gcccgatcag ctttcggcca cgttgcgcca
     4021 gcgcatcttc gataccgccg agaagatcgg atacgccggt ccgaacccgg tcgcgcgcgg
     4081 gttgcggcgc ggcacctcca gcctgatcgc cgtcctcctc gggcaaccgc tcgccgccgc
     4141 atatgacgac cccgtgaccg ccgc
//
LOCUS       DASSGX010000114         3751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_565438, whole genome shotgun sequence.
ACCESSION   DASSGX010000114 DASSGX010000000
VERSION     DASSGX010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565438"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="VFI12_02055"
     CDS             complement(<1..608)
                     /locus_tag="VFI12_02055"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET7055212.1"
                     /translation="MSGHSKWSTIKRQKGATDAKRGQLFTKLAREITVAARTGLPDPE
                     ANGRLRLAIQRARAENMPRDNIERAIERASAAGAGDNFEEVTYEAFLPGGIALMVQSQ
                     TDNRNRTVGEVRSVITRGGGNVGADGSVAWMFDNVGQIVVKADGMSEDDMAMMAIDAG
                     ATEFETDSDTTVVFTEPTDLHRVQEALGAAGLEIESADLVMKA"
     gene            916..2070
                     /gene="rpoD"
                     /locus_tag="VFI12_02060"
     CDS             916..2070
                     /gene="rpoD"
                     /locus_tag="VFI12_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HET7055213.1"
                     /translation="MVSSLLDKGRDQGFVLSDDIMAMYPNAEDHVETIDGLYSWLLQE
                     GIEVLDRAPAVPQEADDQVQAEEQAEVDEKALAHEAGIPAVGDSVRLYLHEIGATDLL
                     TMEEEVWLSKRIELGRFAEERLREADLPLLERRKLEAIRLDGSAARDHLIQANLRLVV
                     SVAKRYVGRGMSFLDLIQEGNIGLMKATEKFDYKRGFKFSTYATWWIRQAITRAISDQ
                     SRTIRLPVHVGETINRVRKIAHRLQQILEREPTEEEVARALDLSSERVRQILDHSRTP
                     ISLEAPVGSEGDAFLGDFIEDDKLPQPIELASHQLMKEQIAEALGKLSERERKIIELR
                     FGLCDGKYRTLEEVGREFGITRERIRQIEAKALRKLRHPSYSRRLRGYLE"
     gene            complement(2403..3647)
                     /gene="fabF"
                     /locus_tag="VFI12_02065"
     CDS             complement(2403..3647)
                     /gene="fabF"
                     /locus_tag="VFI12_02065"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HET7055214.1"
                     /translation="METRRRVVVTGLGAISPVGNTIEESWQSVIAGRSGVATITRFDP
                     TDYETRFAGEVRGFEPDAVLGRKEARRMDRHAQLGVAAALAAREHAGLAEASIDPVRV
                     GLLMGTGMGAMETLEQGAETLLTKGPGRIGPFFAPMALPNMAAGMGAIFLGAKGPCFG
                     SVSACAASAHAIGESVEMIRLGRADVMYAGGAEAPVTRLSVAGFGSMGALSTRNDAPE
                     RASRPFDADRDGFVLGEGAAVLVLESLDHARQRGATILAEVVGYAATDDANHMVQPAP
                     DGEGVARAMRLALADAGMQPTDIGYVNAHATSTRIGEKYETQAIKSVFGDYAYQLPVS
                     STKSMTGHLLGAAGALEAAFCIQAMRTGCLPPTINYETVDPDCDLDYVPNTAREASVR
                     AVMNNSLGFGGHNVSMIFKANL"
BASE COUNT          644 a         1289 c         1176 g          642 t
ORIGIN      
        1 gccttcatga ccaggtcggc cgactcgatc tcgagcccgg ccgcgcccag cgcctcctgg
       61 acccggtgca ggtctgtcgg ttcggtgaac acgaccgtcg tatcgctgtc cgtctcgaac
      121 tcggtcgcgc cggcatcgat cgccatcatc gccatgtcgt cctccgacat gccgtccgcc
      181 ttgacgacga tctggccgac gttgtcgaac atccaggcca cgctgccatc cgcgccgaca
      241 ttgccgccgc cccgcgtgat gaccgagcgg acctcgccga ccgtgcggtt ccggttgtcg
      301 gtctgcgact ggaccatcag ggcgattccg ccgggcagga atgcctcgta ggttacttcc
      361 tcgaagttgt ccccggcccc cgcggcactg gcacgttcga tcgcacgctc gatgttgtcc
      421 cgcggcatgt tctccgcccg ggcccgctgg atcgcgaggc gcaggcggcc gttggcctcg
      481 gggtcaggca gtccggtccg cgcggcgacg gtgatctcgc gcgcgagctt ggtgaagagc
      541 tggccccgct tggcgtcggt cgcgcccttc tgtcgcttga tcgtcgacca tttggaatgt
      601 ccagacatgg gagaaacctt cggcgaaagc gcgggtgatc tacggcaaat gagtatatca
      661 ccgggatatt cgcgaactgg tccggaccag cccttgtcat caagtggtcc ggaccactac
      721 tatgtaacca attcaccaga actttgaaac ttgtagcatg acatcgtaga ctgtcaacaa
      781 ttcgcatatt gagatatcgc cggatgcagt tatgacgatg acttgccccg gcgatccgta
      841 cctatcccag gcacgggaca cggcgatcga ctggcgggag aacggactcc cttaaacgac
      901 ggggcgcgtg cagcgatggt ttccagtttg ctcgacaaag gccgggatca gggcttcgtg
      961 ctgtcggacg acatcatggc gatgtatccg aacgccgagg atcacgtaga gaccatcgac
     1021 ggcctctata gctggctcct ccaggaaggc atcgaggtcc tcgaccgcgc ccccgcggta
     1081 ccgcaggaag ccgacgacca ggtccaggcc gaagaacagg ctgaggtcga cgagaaggcg
     1141 ctcgcgcacg aagccggcat cccggcggtc ggcgattccg tccgcctgta cctccacgag
     1201 atcggcgcga ccgacctgct gaccatggag gaagaggtct ggctctcgaa gcggatcgag
     1261 ctcggccgat ttgccgagga gcgactcagg gaagccgatc tgcccctcct cgagcgccgc
     1321 aagctcgaag cgatccgcct cgatggctcc gccgcgcgcg atcacctcat ccaggccaac
     1381 ctccgcctcg tcgtctccgt cgccaagcgc tacgtcggcc ggggcatgtc cttcctggac
     1441 ctgatccagg aaggcaacat cggcctcatg aaggccaccg agaagttcga ctacaagcgc
     1501 ggcttcaagt tcagcaccta cgcgacctgg tggattcgcc aggcgatcac ccgcgcgatt
     1561 tccgaccaga gccggacgat ccgcctccca gtccacgtcg gcgagacgat caaccgggtt
     1621 cgcaagatcg cgcatcggct ccagcagatc ctcgagcggg agccgaccga agaggaagtc
     1681 gcccgcgcgc tcgatctttc cagcgagcgg gtccgccaga tcctcgatca ctcccgcact
     1741 ccgatctcgc tggaggcgcc ggtcgggtcc gagggcgatg cgttccttgg cgatttcatc
     1801 gaggacgaca agctgccaca gccaatcgag ctcgcctctc accagctcat gaaggaacag
     1861 atcgccgagg cgctcggcaa gctctccgag cgcgagcgca agatcatcga gctccggttc
     1921 ggcctgtgcg acggcaaata ccgcacgctc gaagaggtcg gccgcgagtt cggcatcacc
     1981 cgcgagcgca tccgccagat cgaggccaag gccctccgca agctccgcca cccgtcttac
     2041 agccgccgcc tccggggtta cctcgaatag tcggcaggcg tcggacttcg gacgtcagtt
     2101 gagttgatga gaggggcagg cgcaatctgc ccctttttcg tgacaccgac tgtaggggtt
     2161 gctgccgcag accctccctc cccaactggg ccgttgggtg acgccgaacg tgtaggggcg
     2221 tgcgggggca cgcccgcgcg gtgccgggcg gccggttcgt ggtcggaacg atgccaatcc
     2281 gcgggttctc cccatcttgc gggtctgggg gaccacgacg gatttgcccc cgcaaatccc
     2341 tacaattgcg ggtgcatggc aatgggtgac gcggaggcac gcgggtttgc ggcagctgac
     2401 ccctacaggt tcgccttgaa gatcatcgag acgttgtggc cgccgaagcc gaggctgttg
     2461 ttcatgacgg cccgaacgct cgcttcccgt gccgtgttgg gaacgtaatc caggtcgcaa
     2521 tcggggtcga ccgtctcgta gttgatcgtc ggcgggaggc aaccggtacg catcgcctgg
     2581 atgcagaacg cggcctcgag cgccccggcc gccccaagca agtggccggt catcgacttg
     2641 gtcgacgaca ccggcaactg gtaagcgtag tcgccgaaga ccgacttgat cgcctgcgtc
     2701 tcgtacttct cgccgatcct ggtcgaggtg gcgtgcgcat tgacgtagcc gatgtcagtc
     2761 ggctgcatcc cggcatcagc cagcgccagg cgcatcgccc gggcgacgcc ctcgccgtcc
     2821 ggcgcaggct ggaccatgtg gttggcgtca tccgttgccg catagccgac gacctcggcc
     2881 aggatcgtcg cgccgcgctg ccgagcgtgg tccagcgatt cgaggaccag caccgccgcg
     2941 ccttcgccga gcacgaatcc gtcgcgatcg gcatcgaacg gccggctggc gcgctccggc
     3001 gcatcgttcc gggtagaaag cgcgcccatc gacccgaacc cggccacgct taaccgcgtc
     3061 acgggcgcct ctgcaccgcc cgcatacatg acatcggctc ggccaagtcg aatcatctcg
     3121 acactctcgc cgattgcgtg ggccgacgcc gcgcaggccg agaccgatcc gaagcacggc
     3181 cccttcgcgc cgaggaagat cgcgcccatt ccggcggcca tattcgggag ggccatcggc
     3241 gcgaagaacg gcccaattcg gcccggaccc ttggtcagca gcgtctcggc gccctgctcc
     3301 aacgtttcca tcgcacccat gccggtgccc atcagcaggc cgacccgaac cggatcgatg
     3361 gacgcctcag cgagaccggc atgctcccgg gccgcgagtg ccgccgcgac gccgagctgc
     3421 gcgtggcggt ccatgcggcg agcttccttg cgaccgagga cggcgtccgg ctcgaagccc
     3481 ctgacctcgc cggcgaagcg agtctcgtag tcggtcgggt cgaaccgcgt aatcgtggcg
     3541 acgccggaac ggccggcgat caccgattgc cagctctcct cgatcgtatt gccgaccggg
     3601 ctgatcgccc ccagccccgt gactactact cgtcgccgtg tctccatcgc ctgttcctcg
     3661 tcgcgtagcg cacccctcaa gccgaggatg ctaccacgcg gaatcggttg ccctgtggtt
     3721 ctggcccgtg ctaggctcag taaacggtga g
//
LOCUS       DASSGX010000115         3442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_565497, whole genome shotgun sequence.
ACCESSION   DASSGX010000115 DASSGX010000000
VERSION     DASSGX010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565497"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="VFI12_02070"
     CDS             <1..536
                     /locus_tag="VFI12_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055215.1"
                     /translation="EVGLVEVRTSGRTRVYRAVGERAVPLLNAVKRAAGVDDGPAPSP
                     AALREINRNTPYRNARSCYDHLGGLAGVELLDQFVFDRWLVRNGGPDERPIYEPTDTG
                     EAAFAKLGIDLDLIRTGRRVLACGCLDWTERRPHLGGGLGAAILSALESGGYVERNPE
                     TRGVQIVRSLDDWLAKA"
     gene            complement(572..1213)
                     /locus_tag="VFI12_02075"
     CDS             complement(572..1213)
                     /locus_tag="VFI12_02075"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HET7055216.1"
                     /translation="MNDQHPRIVLASTSPRRRDLIQALGIPVAIEPSRTSEEVAPGTS
                     PVAMVSELAVRKAASIAEQRRGAIVIGADTTVEIDGQILNKPDDARDAERMLRSLRGR
                     QHQVHTGVAILAPDRAPVTEVMTSAVLMHPFSDGELHAYLATGESLDKAGAYGIQGAA
                     GDIVESVTGCYTNVVGLPLCSVARCLIAAGISITVSSPRCGFRTARHCPYWPT"
     gene            1409..2734
                     /locus_tag="VFI12_02080"
     CDS             1409..2734
                     /locus_tag="VFI12_02080"
                     /inference="COORDINATES: protein motif:HMM:NF014135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HET7055217.1"
                     /translation="MSDLTIASIIFIVTYAVIISERLERAVVALAGAAFMIAFGVLTQ
                     HEALESIDPNTLALLIGMMIIINVLKRTGVFRYVGWKTAIALSGDPWRMMLGFTLFTA
                     VASAFLDNVTTILLMVPVTIAICDDLGLDPRPFMITQVIASNIGGAATLIGDPPNILI
                     GSATGLDFVAFLTNLAPLVVILIPFLVAGFWLIYGRSGRLGEPSSEGKKALAFANAAE
                     FIGDAGLLRRSLVVLGMTIAGFVLHGVLGYEAGTVAMFGAVLLLILSRVELHPILAEV
                     EWPTIFFFIGLFILVGGIEQVGLLDKIARRSVSLTGGNVVLTALVLLWFAGIASALVD
                     NIPAVATLIPLTFSVARLLFPELSHLGDAEFATNPSVTPLWWALALGACLGGNGTLVG
                     ASANVVAVGIAERRGEPIGFWGFTKIGAPVAVLSLLVSSLYIWLRYFAF"
     gene            complement(2747..2971)
                     /locus_tag="VFI12_02085"
     CDS             complement(2747..2971)
                     /locus_tag="VFI12_02085"
                     /inference="COORDINATES: protein motif:HMM:NF024645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET7055218.1"
                     /translation="MRRNEAADNARVINCPACGEHLWVQDRFCPECGAQVPVPPAEDF
                     PSTPAPQPPTESGDASDSGTPRWRIWRRDS"
     gene            3257..>3442
                     /locus_tag="VFI12_02090"
     CDS             3257..>3442
                     /locus_tag="VFI12_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055219.1"
                     /translation="MPSGAGRDGGTGFCDLVAGILGGTRCSLLVARADGALEVEEAVG
                     LPIGIDAPAGDSVGDRVA"
BASE COUNT          552 a         1067 c         1136 g          687 t
ORIGIN      
        1 gcgaggtggg cctcgttgag gtgcgaacca gcggccgcac ccgggtctac cgggccgtcg
       61 gcgagcgagc tgttccactt ctgaatgcgg tcaagcgcgc ggctggtgtt gacgatggcc
      121 cggcgccgag ccccgccgct ctccgcgaga tcaaccgcaa cacgccgtat cgcaacgcgc
      181 ggagctgcta cgaccacctg ggtggtctgg ccggggtcga gctgctcgac cagttcgtct
      241 ttgatcgctg gctggtgcgc aatggcggcc cggatgagcg gccgatctat gagccgaccg
      301 acaccgggga agcggccttc gcgaagctcg ggatcgacct ggacctgatt cggactggtc
      361 ggcgggttct ggcctgtggc tgcctggatt ggaccgagcg ccggccgcat cttggcggtg
      421 gtctcggcgc ggcaatcctg tcggcactgg aatccggcgg ctacgtcgag cggaatccgg
      481 aaacacgagg tgtgcagatc gtgcgcagcc tcgatgactg gctcgccaag gcctgagaat
      541 gtgatcgctc ggaactacgt tgcgtttgtg gtcacgttgg ccagtagggg cagtgccggg
      601 cagtccgaaa tccgcagcgc ggcgaggata cggtgatcga aatgcccgcc gcgatcaggc
      661 agcgcgcgac cgagcaaagc ggcaatccca cgacattcgt gtaacacccc gtcaccgatt
      721 ccacgatatc gccggccgcg ccctggatcc cataggcccc ggccttgtcg agcgattctc
      781 cggttgccag gtatgcgtgc aattcgccgt cggagaatgg gtgcatcagc acggcggacg
      841 tcatcacttc tgtcaccggc gcccgatcgg gggcgaggat ggcgacgccg gtgtggacct
      901 ggtgctggcg gccgcgcagg cttcgcaaca tgcgttcggc atccctggcg tcatccggct
      961 tgttcagaat ctggccgtcg atctcgaccg tggtatcggc gccgatcacg atagcgccac
     1021 gacgctgctc cgcgatcgac gccgccttcc ggaccgccag ctcgctgacc atcgccacgg
     1081 gagatgttcc cggcgcaacc tcttccgacg tacgcgacgg ctcgatggca accgggatgc
     1141 caagagcctg gatcagatcc cgacgacgag gagatgtgga tgcgagcacg attcgcgggt
     1201 gctggtcatt cattggcaca caccggcgga gagtcgcgtt gcatctatac tgctcccgga
     1261 tctgacatag tcacgaatcc ggggtcgccg tgcatccctt atccgcatgg ctatggaggg
     1321 gactgccaat tggcggtccc ttttttgttt ccacacatgc cgctcacttg ccgtgtggcc
     1381 gagcggtggc tgttgtcgcg ggaatgccat gtcggatttg accatcgctt cgatcatctt
     1441 cattgtcacc tacgccgtca ttatcagtga gcggctggag cgggcggtgg tggcgctcgc
     1501 cggcgcggcg ttcatgattg cattcggcgt cctcacgcag cacgaggcac tcgaatcgat
     1561 cgacccgaac accctggccc tgctgatcgg gatgatgatc atcatcaatg tcctcaagcg
     1621 aaccggcgtc ttccgctacg ttggctggaa gacggcgatc gcgctcagcg gcgacccgtg
     1681 gcggatgatg ctcggattta cgctattcac cgccgtcgca tctgcttttc tcgacaacgt
     1741 gacgacaatc ttgctcatgg tgccggtcac catcgccatc tgcgatgacc tgggcctcga
     1801 tccgcgcccg ttcatgatca cgcaggtcat cgccagcaac atcggtggcg ccgccacctt
     1861 gatcggcgat ccgccgaata tcctgattgg cagcgcgacc ggactcgact tcgtggcctt
     1921 tctgaccaac ctcgcgccac tggttgtcat cctgatcccg ttcctggtcg ccggattctg
     1981 gctgatctac ggacggagcg gccgccttgg cgagccatcg tcggaaggca agaaggcgct
     2041 tgcctttgcg aatgccgccg agttcatcgg cgatgccggg ctccttcgtc gctcgcttgt
     2101 cgtactcggg atgacgatcg ccggtttcgt gctgcacggc gtgctgggtt atgaggcggg
     2161 cacggtcgcg atgttcgggg cggtcctgct cctgatcctg agccgcgtcg agttgcatcc
     2221 gatcctggca gaggtcgagt ggccgacgat cttcttcttt atcgggctct tcatcctggt
     2281 cggcggcatc gagcaggtcg gactgcttga caagatcgcc cggcgcagcg tctcgctgac
     2341 gggcggtaac gtggtcctga ccgcgctcgt cctgctttgg ttcgccggaa tcgcctcggc
     2401 gctggtcgac aatatcccag ccgtcgccac gctgatcccg ctgacatttt ctgtggcgcg
     2461 gctgctgttt cctgagctct cgcaccttgg cgatgccgag tttgcaacga atccgagcgt
     2521 gaccccgctc tggtgggcgc tcgcgctcgg ggcctgcctt ggtggaaacg ggacgctcgt
     2581 cggcgcgtcg gcgaatgtgg tcgcagtcgg aatcgcggaa cggcggggcg agccgatcgg
     2641 gttctggggc ttcacgaaga tcggcgcccc ggtcgcggtg ctcagcctgc tcgtatcgag
     2701 cctctacatc tggctgcgct acttcgcctt ctagaacggg cgctggtcag gagtcgcggc
     2761 gccagatgcg ccagcggggc gtccccgaat cggacgcgtc gccagattcg gttggcggtt
     2821 gcggcgccgg agtggatggg aagtcctcgg ccggcgggac cggcacttgc gcgccacatt
     2881 ccgggcagaa gcggtcctgg acccagagat gctcaccgca ggctgggcag ttgatcacgc
     2941 gcgcattgtc ggccgcctcg ttccgtctca tgggacaagc atcgtcgagc ggagcaccgg
     3001 gcgcaactga gtgccggcaa ttctaccgag aaatgaagga atgcaaagga atgggtagaa
     3061 ataaccagaa ttactaggta tactcgaccg tacatagtcc gaaagtacta gccaccccgc
     3121 atgcggcccg acgaattcga cgaggaacag tccgctggtc tgctaccagg tggggacgga
     3181 ttattcatcc cggttaggtg gtgctgccat ggtgcaacgg ttcgctactt cgtcgtctgg
     3241 acctggcagg gaaagcatgc cctctggagc tggtcgcgac ggcgggaccg ggttttgcga
     3301 cctggtcgcc ggcatccttg gaggaactcg ctgctcgctg ttagttgcgc gggctgatgg
     3361 ggcactcgag gtcgaagagg ccgtcgggtt gccgatcggc atcgacgcgc cagccggcga
     3421 ttctgtcggc gatcgcgtgg cc
//
LOCUS       DASSGX010000116         2017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_347984, whole genome shotgun sequence.
ACCESSION   DASSGX010000116 DASSGX010000000
VERSION     DASSGX010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347984"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1282
                     /locus_tag="VFI12_02095"
     CDS             <1..1282
                     /locus_tag="VFI12_02095"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7055220.1"
                     /translation="MVGQSIALRARAGMFGDRNFRLIWSAATVSAFGYYITDIAIPLL
                     AIEQLDVNSFEIGLIRVIQQLPNLFFGLFLGVLVDRVRKRPLMITADIVRAFVLLAIP
                     MAAFWNVLNLPIVLAVVFVVGSFNLLFDVSEGSIIPLVVPRSRLVDANAKIEASFSTA
                     QTAGPAIGGALVSVLTAPYAVLVTALTLGGSASFLSRMTTHEPRPHVDQERSVRQDIA
                     EGIRFVRHHAILRPILLTMIGQSFFGFVFMAVYVLYMKRNLGISDFQVGLVFAAGGIG
                     SLLGTLATPALNRRFGVGPVIVAGNLFFGVTGMLIPLAVLVPGYAFPLVIVCEFLQYL
                     GLIPFFLNAISLIQLEAPDHLRGRVMSTRKFLTWGVQPFGSLLGGVLGGLITVPWTLA
                     AGEFGLLAVGIWLMFHPIRSMRSLHAEGDGADGV"
     gene            1272..2000
                     /locus_tag="VFI12_02100"
     CDS             1272..2000
                     /locus_tag="VFI12_02100"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HET7055221.1"
                     /translation="MASDLSHARSNRSINGDPVKIELPRPRPERITIKGQRVTVEPLD
                     AEKHAAELFPLLHGDPEKERVWDHLPYGPFPSLDAYRDWVAEVTSRDDPLFYAIRNTR
                     TGRVEGVASLMEIRPAAGVIEIGHINLSPALQNTIEATEGLYLLMAYAMDELGYRRHE
                     WKCNALNANSRRAAGRLGFSFEGIFYQHSVPKGHNRDTAWFSILDYEWPAIRQNFETW
                     LAPDNFDENGRQRQSLGEMNRSIP"
BASE COUNT          315 a          640 c          670 g          392 t
ORIGIN      
        1 catggttgga caatccatag cgctgcgtgc gcgcgccggc atgttcggcg atcgcaactt
       61 tcgcctgatc tggagcgcgg cgaccgtctc cgcgttcggc tactacatca ccgacatcgc
      121 gatcccgctg ctggcgatcg agcagctgga cgtgaactcg ttcgagatcg gcctgatccg
      181 ggtcatccag cagttgccga acctcttctt cgggctgttc cttggcgtgc tcgtcgaccg
      241 cgtccggaaa cggccgctga tgatcacggc ggacatcgtc cgcgcgttcg tgctgctggc
      301 gatcccgatg gcggcgtttt ggaacgtgct gaacctgcca atcgtgctgg cggtcgtctt
      361 cgtggtcggt tcgttcaacc tgctcttcga cgtcagcgag ggctcgatca tcccgctcgt
      421 ggtcccgagg agccggctgg tcgatgccaa cgcgaagatc gaggcgagct tttcgacggc
      481 gcagacggcc ggtcctgcta tcggtggggc actggtcagt gtcctgaccg cgccgtacgc
      541 cgtgctggtc acggcgctga cattgggcgg gtcggcctcg ttcctgagcc ggatgacgac
      601 gcacgagcca cggccgcatg tcgatcagga gcgctcggtg cgccaggaca tcgccgaggg
      661 gatccgcttc gtgcggcacc acgcgattct gcgaccgatc ctgctgacca tgatcggcca
      721 gagcttcttc ggtttcgtct tcatggcggt ctacgtgctc tacatgaaac gaaacctggg
      781 gatcagcgac ttccaggtcg gattggtctt tgcggccggc gggatcggct cgctgctcgg
      841 gacgttggcg acacccgccc tcaaccggcg attcggggtc ggaccggtga tcgtggccgg
      901 caacctcttc ttcggcgtga ccgggatgct gatcccgctg gcggtgcttg tcccgggcta
      961 cgcgttcccg ctggtcatcg tttgcgagtt cctgcagtac ctcgggctga tccccttctt
     1021 cctgaatgcg atctcattga ttcagctcga agcgccggac catttgcggg gccgcgttat
     1081 gtccacccgc aagttcctga cctggggcgt gcagccgttc ggttcgctat tgggcggcgt
     1141 cctgggcggg ctgatcacgg taccatggac gctggctgcc ggagaatttg gcttgctcgc
     1201 ggtcgggatc tggctcatgt tccacccgat ccgctcgatg cgctcgcttc acgcggaagg
     1261 ggacggtgcg gatggcgtct gatctcagtc atgcccggtc gaaccggtcg atcaacggcg
     1321 atccagtcaa gatcgagttg ccccggccgc gtccagagcg gatcacgatc aagggtcagc
     1381 gggtcacggt cgagccgctt gacgcggaga agcatgcggc cgagctcttt cccctgctcc
     1441 acggcgaccc cgagaaggag cgagtctggg accacctgcc gtacgggccg tttccgagcc
     1501 tcgacgccta ccgggattgg gtcgcggagg tcaccagcag ggacgatccc ctcttctacg
     1561 cgatccggaa cacgcggacc gggcgggtcg agggggtggc ctcgctgatg gagatccggc
     1621 cggccgcggg cgtgatcgag atcggccaca tcaacctcag tcctgcgctt cagaacacga
     1681 tcgaggcgac cgaagggctc tacctgctca tggcctacgc gatggacgag ctcgggtacc
     1741 ggcggcacga gtggaagtgc aacgcgctca acgcgaactc gcggcgcgcg gcgggacggc
     1801 tgggcttcag cttcgagggc atcttctatc agcactcggt gccgaagggg cacaaccggg
     1861 acaccgcctg gttctcgatc ctcgactacg agtggccggc gatcaggcag aacttcgaga
     1921 cctggctcgc gcctgacaac ttcgacgaga acggccgaca gcggcagtcg ctcggcgaga
     1981 tgaacaggag catcccgtga cgttgaccaa cgtcggc
//
LOCUS       DASSGX010000117         2117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1110004, whole genome shotgun sequence.
ACCESSION   DASSGX010000117 DASSGX010000000
VERSION     DASSGX010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110004"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..904
                     /locus_tag="VFI12_02105"
     CDS             <1..904
                     /locus_tag="VFI12_02105"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HET7055222.1"
                     /translation="ASDSVIDRIAVGTRPRGVRVSPDSRMVYVALSGSPRCPPTMPDS
                     ECAKLKVDKSKDGIGEVDVATRQLRRVLPGGSDPENFDLSPDGTTLYTSNEDANTASF
                     IAINGGTVLRTVPVGREPEGVAVPPDGKTVWVTSESDNVVTVLDAESGAGVARISVGK
                     RPRDIVFSSNGTRAYVSAEAGGNISVVDAAKFRVLRTIPMPEGARPMGLALMADGKQL
                     WVANGRAGTVSLLDLESDTILANVKVGVRPWGLALLPDGTRLYVANGPSGDVSVINTA
                     TRQVARTIATGELPWGAAVARVTE"
     gene            complement(932..1705)
                     /locus_tag="VFI12_02110"
     CDS             complement(932..1705)
                     /locus_tag="VFI12_02110"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HET7055223.1"
                     /translation="MNHTIIFAPALLLTLAACPSHGPRNDASADAVKNDTMSHSVSTT
                     ASDCGDLPSSDSLRAMLRTAPDSGEAGGLFGGRAEWGAIVNRSGKVCVVVPPSDSVGG
                     RWPGSRSISMAKAFTANGFSTDTLPLSTARLYTLTQPGHSLWGVSQPEPFDPACLDPG
                     HEPGICGGAIAFGGGVPLYRSGHVIGGLGISGDTPCADHEIAKRIRGMAGLAPSTGNT
                     ADDITYSKVDHASIYTHPLCPNTWRNGKKIGDETVASGY"
     gene            complement(1775..>2117)
                     /locus_tag="VFI12_02115"
     CDS             complement(1775..>2117)
                     /locus_tag="VFI12_02115"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HET7055224.1"
                     /translation="IAIGPQPGPDQALDTLHNPYANDTGILTDARRLFVWYNCAGCHG
                     DHGGGGMGPSLRDSTWLYGGSDADIYASIAEGRANGMPAWGTKLPADQIWQVVTYIQS
                     LRTPNEPEKPR"
BASE COUNT          383 a          674 c          689 g          371 t
ORIGIN      
        1 tgcaagcgac agcgtcatag accggattgc cgttggcacc cggccccggg gcgtccgggt
       61 gagcccggac agccgtatgg tgtacgtggc actcagcggc tcgccacggt gcccgccaac
      121 gatgccggat agcgaatgcg ccaagctcaa ggtcgacaag tccaaggatg gaatcggcga
      181 ggtggatgtc gccacgcggc agctgcgacg ggtactgccc gggggttccg atcctgagaa
      241 tttcgacctg agtcccgatg gcaccacgct ctacacgtcc aacgaagatg ccaacaccgc
      301 atcgttcatt gctatcaatg gcggcactgt cctgcgtacg gtgcctgtgg gacgcgagcc
      361 ggagggcgta gcggttccgc ctgatggcaa gacggtctgg gtgaccagcg aatctgacaa
      421 cgtcgtgacc gtactcgatg ccgaatcagg cgccggggtg gcccgcatct ccgtcggcaa
      481 gcgcccgcgg gatatcgttt tctcgagtaa tgggacgcga gcctatgtga gcgccgaggc
      541 gggcggcaac atctccgtgg tggacgccgc gaagtttcgc gtgctccgca ccatcccgat
      601 gcccgagggc gcgagaccaa tgggactcgc cctcatggct gatgggaagc agctctgggt
      661 ggccaacgga cgggccggga cggtctcgct gctggacctc gaaagcgaca cgatactggc
      721 gaacgtgaag gtcggcgtcc ggccatgggg actcgcgctc ctgcccgatg gcaccaggct
      781 ctatgtcgcg aatggtccct caggcgacgt gtcggtcatc aacacggcta cgcggcaggt
      841 ggcgcggacc attgccaccg gcgagctacc atggggtgcg gcagttgccc gcgtgacgga
      901 gtagcgctgc gtgaagcgcg aagcgctcaa gctagtaccc ggaagcaacc gtctcgtctc
      961 cgatcttctt cccattgcgc caggtgttgg ggcagagcgg atgggtatag atcgaggcgt
     1021 ggtccacctt gctgtacgtg atgtcgtccg cggtattgcc ggtggacggc gcgagccccg
     1081 ccattcctcg aatgcgcttg gcaatttcat gatcagcaca cggcgtgtcg ccgctgatgc
     1141 cgagcccgcc gatgacgtga ccgcttcggt acagcggaac gcctcccccg aatgcaattg
     1201 ccccaccgca gatgccaggt tcatgccctg ggtccaggca cgcgggatcg aaaggctccg
     1261 gctgcgacac gccccagagg gaatggccgg gctgggtcag ggtatagagc cgagccgttg
     1321 agagcggcag ggtatcagtg ctgaagccgt tcgctgtgaa cgccttggcc atcgagatgg
     1381 agcggcttcc cggccagcgc ccgcccactg aatcactggg tggcaccacg acacacactt
     1441 tccccgaccg gttcacgatc gcaccccatt cggcgcggcc gccaaacagt ccgcccgctt
     1501 cacccgaatc tggtgccgta cgaagcatgg cccgcagcga atcggaactc ggcagatcgc
     1561 cgcaatccga agccgtcgtc gataccgagt gagacatcgt gtcgttcttc accgcgtctg
     1621 cggatgcgtc attccgcggg ccatgactgg ggcaggcggc gagcgtgagg aggagggccg
     1681 gagcgaagat gatggtatgg ttcattgtcg gtgtccttaa tcaacggtca acagtcaacg
     1741 gtcgaccgta gaccgttgac cgtcgaccct ccactcacct cggcttctcc ggctcattgg
     1801 gcgttcgcag ggactggatg tacgtcacca cctgccagat ctggtcggcc ggcagcttgg
     1861 tgccccacgc cggcatccca ttggcacgcc cctcggcgat cgacgcataa atgtccgcat
     1921 cggaaccgcc atagagccac gtcgagtcac gcaggctcgg ccccatcccg ccaccgccgt
     1981 ggtcgccatg acaccccgca cagttgtacc agacaaacag gcgccgtgca tccgtgagga
     2041 ttcccgtgtc gttcgcgtaa ggattgtgca gcgtgtcgag tgcctgatct ggacccggct
     2101 gtggtccgat cgcgatg
//
LOCUS       DASSGX010000118         2540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_893073, whole genome shotgun sequence.
ACCESSION   DASSGX010000118 DASSGX010000000
VERSION     DASSGX010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_893073"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="VFI12_02120"
     CDS             complement(<1..956)
                     /locus_tag="VFI12_02120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HET7055225.1"
                     /translation="MTDSEQNTAGPNGYDALRARAFDFVSQHNGRVTEDDLIRFMFGA
                     ATKPALWRSLLVTVLGNDHRFIQVGNQTWALAGSDQSTSSDVVSFVAVDVETTGLRPT
                     QHRVIEIGMARYVNGRCTERYSTLINPERRVPDYVRKLTGLTDSDLIPAPRFGQIAGE
                     IAAFMGDLPLLGHNVGFDVAFLNAEFERAKRPAIVNQTIDTVPMAMRVLGRSMRPSLD
                     RVATALGLQPRKYHRALADADLSAAVALRLLGMAAESGTTIDALIRSTRAARSLPPGH
                     GQSAGELLDRRHLDVLPRRPGVYLMIDANDRVLYVGKSKSLR"
     gene            1053..>2540
                     /gene="ligA"
                     /locus_tag="VFI12_02125"
     CDS             1053..>2540
                     /gene="ligA"
                     /locus_tag="VFI12_02125"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HET7055226.1"
                     /translation="MSDSAIDHRVAELRAQLDQWNYEYYVLDQPTVSDAEYDEALNEL
                     RAIESEHPELVTTESPTQRVGAAPQTGFSKITHPVQMLSLSNVFSFEELQEWERRLER
                     FVGTRHEYVVECKIDGLAVALTYENGRLLHGATRGDGSVGENITPNLKTIPTIPLKLR
                     GNGLPERIEVRGEVYMRKRDFEALNERIVAAGGNPFMNPRNSAAGSLRQLDPNLTRQR
                     PLRFFAYGIGYINDGWEPASHCEILQRLSEFGFEASPDAARFETLDQVWERFQYWHER
                     RDQLDFEIDGAVVKVDSLRLQDEAGSVGREPRWATAYKFPAIQKTTKLLDIQINVGRT
                     GTLNPLAILEPVNIGGVIIKRATLHNEDEIHRKDIRIGDTVVVQRAGDVIPQIVSVVA
                     EKRTGEEIPFSMPDHCPSCGSPVHREPGEAMHYCTNASCPAQLREHLYHFVSRGAMDI
                     DGLGSKLIDRFVDLGWIRGMADIYSLDWSAVAALEGLGEKSAANLS"
BASE COUNT          483 a          787 c          814 g          456 t
ORIGIN      
        1 tcccggagcg atttggactt gccgacatag agcacccggt cgtttgcgtc aatcatcaga
       61 tagacaccgg gacgcctcgg cagaacatcc aggtgacggc gatccagcaa ttctccggcc
      121 gattgaccgt gcccaggcgg cagcgacctg gcagcccggg tggagcgaat caacgcatcg
      181 atcgtcgtgc ctgactcagc cgccatgccc aacaagcgga gcgcgactgc cgccgacagg
      241 tcagcgtcgg ccagcgcccg gtggtacttg cgcggttgca ggcccagcgc cgtcgcgacg
      301 cgatcgagcg agggccgcat cgaccggccg agcacccgca tcgccatcgg aaccgtgtcg
      361 atcgtctggt tcacgatcgc cgggcgcttg gctcgctcga attcggcatt caggaaagca
      421 acgtcgaaac caacgttatg acccaggagc ggtaaatcgc ccatgaatgc cgcgatctcg
      481 cccgcgatct gtccgaagcg cggcgccggg atcaggtcgc tgtccgtcag gccggtcagc
      541 ttgcggacgt agtcgggaac gcgacgctcg gggttgatca aggtcgaata gcgctcggtg
      601 cagcggccgt tgacgtacct ggccataccg atctcgatca cccgatgttg cgttggacgg
      661 agaccggtcg tctcgacatc gacggctacg aacgagacga cgtcgctgct cgtcgattga
      721 tcggatccag ccagtgccca ggtctggttg ccgacctgga tgaaacgatg atcgtttccg
      781 aggaccgtaa ccaggagcga gcgccagagc gcgggtttgg tcgcggcccc aaacatgaac
      841 ctgatcaggt catcttcggt gacccggcca ttgtgctggg aaacgaaatc gaacgcgcgg
      901 gcgcggaggg cgtcgtaccc atttggtccg gctgtgttct gctcagaatc ggtcaaggga
      961 gtcgtccgtg gtggcggccg ggcgtgttag aatgcttgtt cgtatcctac ggcgagttcc
     1021 cataccgctg caaggctgac ggagttcggt gcatgtccga ttcggcgatt gatcatcgag
     1081 tcgcggagct gcgggcgcag ctcgaccagt ggaactacga gtattacgtc ctcgatcaac
     1141 caaccgtctc cgatgccgaa tatgacgaag ctctgaatga gctgcgggcg atcgagtccg
     1201 agcacccgga gctcgtcaca accgagtcac cgacgcagcg agttggcgcg gccccgcaga
     1261 ccggtttcag caagattacg catcccgttc agatgctctc gctgagcaat gtcttcagct
     1321 tcgaggagtt gcaggagtgg gagcggcgcc tcgaacggtt cgtcggcacg cgtcacgaat
     1381 acgtcgtcga gtgcaaaatc gacgggctcg ccgtcgcgct aacttatgag aacggccggt
     1441 tgctccatgg agcgacgcgg ggcgatggct cggtcggcga gaacatcacg cctaacctca
     1501 aaacgatccc gacgatcccg ctcaagctgc gcgggaacgg tttaccggag cgaattgagg
     1561 tccgcggcga agtctatatg cgcaagcgcg acttcgaggc gctgaacgaa cggatcgtcg
     1621 cggccggtgg caacccgttc atgaacccgc gcaattccgc tgccggctcg ctccgccagc
     1681 ttgatccgaa cctgacgcgg cagcgtccgc tccgcttctt cgcctacggt atcggttaca
     1741 tcaatgacgg ttgggagccg gcgtcgcact gcgagatcct gcagcggctc agcgagttcg
     1801 gctttgaggc atcgcccgac gcggcccgtt tcgaaacgct cgaccaggtt tgggagcggt
     1861 tccagtactg gcatgagcgg cgcgatcagc tcgatttcga gatcgacggt gccgtcgtca
     1921 aggtcgattc gctgcggttg caggacgagg ccgggtcggt cggccgggag ccccgctggg
     1981 caaccgcgta caagttcccc gctatccaga agacgacgaa gttgctcgac attcagatca
     2041 acgtcggccg aaccggtacg ctcaacccgc tcgcgatcct cgagccggtc aacatcggcg
     2101 gcgtgatcat caagcgcgcc acgctccaca atgaggacga gatccatcgc aaggacatcc
     2161 gcatcggcga caccgtcgtc gtccaacggg cgggcgacgt gatcccgcag attgtctcgg
     2221 tagtcgcaga gaagcggacc ggcgaggaga tccccttctc gatgccggat cattgcccgt
     2281 cctgcggctc gccggtgcac cgcgaaccgg gcgaggcgat gcactattgc accaacgcga
     2341 gttgtccggc ccaacttcgc gagcacctct accacttcgt cagccgtggt gcgatggata
     2401 tcgatgggct cggctcgaaa ctaatcgacc gcttcgtcga tctcggctgg atccgcggca
     2461 tggccgacat ctactcgctg gattggtcgg cggtggcggc gcttgagggg ctgggcgaaa
     2521 aaagtgcggc gaacctctcg
//
LOCUS       DASSGX010000119         3484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1110451, whole genome shotgun sequence.
ACCESSION   DASSGX010000119 DASSGX010000000
VERSION     DASSGX010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110451"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="VFI12_02130"
     CDS             complement(<1..734)
                     /locus_tag="VFI12_02130"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HET7055227.1"
                     /translation="MVSEYHDPSVLVSTEWVAEHLTDPTVRIVESDEDILLYDIGHIP
                     GAVKLDWHTDLQDEVRRDFIDKLQFEALVGSVGISNDTTVVFYGDRNNWYATYAFWLF
                     KYYGHEDARIMDGGRAKWEAGGRPYTRAEAKHPHTTYDAKEPDAGVRAFRDDVLKMVE
                     AGSPALVDVRSPQEYTGEVIHMLGYPQEGAQRGGHVKGARNIPWNKAANEDGTFKSVP
                     ELQEIYGTKGITADKDVIAYCRIGERS"
     gene            complement(841..1767)
                     /locus_tag="VFI12_02135"
     CDS             complement(841..1767)
                     /locus_tag="VFI12_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055228.1"
                     /translation="MNALIVDPTNTVEAVLSPTLAERGWHVDHARHVYEIYDLVMKNP
                     PDLIVVHGDGAGGEAAHICQRFKQNVLTAGIPIILVEDSPPPAWMIAGLPADAILQAP
                     WETEEFFHHVEVLLPGQSVTSSLDDLTNFPRRASVIDEMQRRVLARELFGVGVLNLRE
                     ADAYRQDFGRSGLDQFVVLVSVLLRRHAAASTPISVGHLDDGNFLVIGPNSAVQEVIA
                     QTTYEFDALVPAYYEMDTLFGADDSHDVGPSTWIALQGALCLVEPDGFENILQIGYRL
                     SDTLTAGADAIHSLSMDALAEGTSALTGAIQA"
     gene            complement(1857..2804)
                     /gene="miaA"
                     /locus_tag="VFI12_02140"
     CDS             complement(1857..2804)
                     /gene="miaA"
                     /locus_tag="VFI12_02140"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HET7055229.1"
                     /translation="MTSPHHSLVIAIFGPTGVGKTDAAIDLALRYNGEVVNADSRYLY
                     RHLTIGVAKPTLEERRGVRHHLIDIFEPAEFITIAQVQRLAYTAIDDILERGRLPILV
                     GGTPLYMNAIVEGWRIPEVAPDWPLRDELSARIEREGLEPVVAELARVDPVAAERSGQ
                     NPRRVIRALEIYRVTGVPMSELEGKDPPPYDILKIALTRDREDLYRILDKRVHEQVAA
                     GLLDEVRGLLEMGMSGNEPAFSSIGYRQLLPVLTGDQPLDDAIVRIKHDTHRYVRHQM
                     TWLRRTPGLHWLDTGTPGWQRELDARVTARIAATSTSES"
     gene            2950..>3484
                     /locus_tag="VFI12_02145"
     CDS             2950..>3484
                     /locus_tag="VFI12_02145"
                     /inference="COORDINATES: protein motif:HMM:NF014656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HET7055230.1"
                     /translation="MIRFVVSSLKIAVIVISTAAVALGGFQLFDHYSSRAEEQSGVGE
                     PVVFTIGKNDEAGDVAKNLRKKGLIRSEQVFELTVRYVDRDIKPATYELTKGMSVSTI
                     VDLITTEKSKAVTKLKEYKITVVEGWRTEQIADELDKLKYPPGGDAFLRAAEEYPHDS
                     YDFLKGTKKGTLEGFLYP"
BASE COUNT          620 a         1071 c         1165 g          628 t
ORIGIN      
        1 gagcgctcgc cgatgcggca gtaggcgatg acgtccttgt cggcggtgat gcccttcgtc
       61 ccgtagatct cctggagctc cggcacggac ttgaaggtgc cgtcctcatt ggcggccttg
      121 ttccagggga tgttgcgcgc gcccttgacg tggccgccgc gctgcgcgcc ctcctgcgga
      181 tacccgagca tgtggatgac ctcgccggtg tactcctgcg gcgagcggac gtcgaccagc
      241 gccgggctgc cggcctcgac catcttcagc acgtcatcgc ggaaggcgcg gacgcccgcg
      301 tccggctcct tggcgtcata ggtcgtgtgg gggtgcttcg cctcggcgcg ggtgtatggc
      361 cggcctcccg cctcccattt ggcgcggccg ccgtccatga tccgcgcgtc ttcgtggccg
      421 taatacttga agagccagaa ggcgtaggtc gcgtaccagt tgttgcggtc gccgtagaac
      481 acgacagtcg tatcgttgct gatgccgaca ctgcccacga gcgcctcgaa ctggagcttg
      541 tcgatgaagt cgcgccggac ctcgtcctgc aaatcggtgt gccaatccag cttgaccgcg
      601 cccgggatgt ggccgatgtc gtagagcagg atgtcctcgt ccgattcgac gatgcggacg
      661 gtcggatcgg tcaaatgctc ggccacccac tcggtggaga ccagaaccga cggatcgtga
      721 tattcgctga ccataaagac ggcctccagg gcgcggggac ggcgggaatg cggctcaacc
      781 ggcacacgac cggtaagcag cttccaccag tatacggctg aattagtcag cgattgagta
      841 tcaggcctgg atggcgccgg tcaatgccga cgtgccctct gccaaagcgt ccatggagag
      901 cgagtggatc gcgtccgcgc cggcggtgag ggtgtcggac aggcggtagc cgatctgcag
      961 gatgttctcg aacccgtccg gctcgaccag gcagagcgcg ccctggagcg cgatccaggt
     1021 cgacggtccg acatcgtgcg agtcgtccgc gccgaagagg gtgtccatct cgtagtaggc
     1081 cgggacgagc gcgtcgaact cgtaggtcgt ctgggcaatc acttcctgga cggccgagtt
     1141 cgggccgatg acgaggaagt tgccgtcgtc gaggtggccg acgctgatcg gcgtcgacgc
     1201 ggcggcatgg cgacggagca gcacgctgac cagcacgacg aactggtcca gcccgctcct
     1261 gccgaagtcc tgccggtagg cgtccgcctc gcgcaaattg agcacgccga cgccgaacag
     1321 ctcgcgggcc aggacccggc gctgcatctc gtcgatgacc gacgcgcggc gcgggaagtt
     1381 cgtcaggtcg tcgagcgagc tggtcaccga ttgaccgggc agcaacacct caacgtggtg
     1441 gaagaactcc tcggtttccc acggcgcctg cagaatcgca tcggcgggca gccccgcgat
     1501 catccaggca ggcggcgggg agtcctcgac gaggatgatc gggattccgg cagtcaggac
     1561 attctgcttg aaccgctggc agatgtgcgc tgcctcgccg cccgcaccgt cgccgtggac
     1621 gacgatcaga tcgggcggat ttttcatgac caggtcgtag atctcgtaca catggcgcgc
     1681 gtggtcgaca tgccagcccc gctccgccag agtaggagac agcacagcct cgaccgtatt
     1741 cgtcggatct acgatcagtg cattcatggg cagcgggcaa tcctccaggt tcttcgcctt
     1801 gccgatcatg gtaggcggtg ccgggcacgc gggaaacgtg ccgtatcacc ccaaagtcag
     1861 gattcgctgg tactcgtggc cgcgatgcgc gccgtcaccc gcgcgtcaag ctcgcgttgc
     1921 cagccgggtg tgccggtgtc gagccaatgc aggccggggg tacggcggag ccaggtcatc
     1981 tggtgacgca cgtagcgatg ggtgtcgtgc ttgatgcgga caatagcgtc gtcgagcggt
     2041 tggtcgccgg tgagaacggg caagagctgg cggtagccaa tcgaggagaa ggccggctca
     2101 ttgccgctca tccccatctc gagcaagccc cgcacctcgt cgagcaggcc ggcggcaact
     2161 tgctcgtgga ctcgtttgtc gaggatccga tacaggtctt cgcggtcgcg cgtcagggcg
     2221 atcttgagaa tgtcgtaggg tggcggatcc ttgccctcca actcgctcat cggcacgccc
     2281 gtcacgcggt aaatctcgag cgcgcgaatg acgcggcggg ggttctggcc cgatcgctcg
     2341 gcggccaccg gatcgacccg ggccagctcc gcgacgactg gctccaaccc ctcgcgctcg
     2401 atccgggcgg acagctcgtc gcgaagtggc cagtccggcg cgacctcggg aatgcgccag
     2461 ccctcgacga tcgcgttcat atagaggggc gtgccgccga ccaggatcgg caatcgaccg
     2521 cgctccagga tgtcgtcgat cgccgtgtag gcgagccgct ggacctgcgc gatggtgatg
     2581 aactcggctg gttcgaagat gtcgatcagg tggtggcgaa ccccacggcg ctcctcgagt
     2641 gttggtttag cgacaccgat ggtgaggtgg cggtagagat agcgggaatc cgcgttgacc
     2701 acctcgccgt tgtaccggag ggcaagatcg atggcggcat ccgtttttcc gacgccggtc
     2761 gggccgaaaa tggcaatgac gagtgagtgg tgagggctgg tcatcgaaac tcgctgctag
     2821 tcctgtgcgc ttatgggaag tagactggtg cgcgcggata tcattattgc gccgcatccg
     2881 cgagtatccc gcctgatctc cccatgtgca ggtgtcttcg tccgagcgag gttcgggagc
     2941 acgagttaaa tgatccgatt tgtcgtttcc agtctcaaga tcgcggtgat cgtgatcagc
     3001 accgccgcgg tcgcgctggg cgggtttcag ttgtttgacc attactccag ccgggccgag
     3061 gaacaatccg gcgtcggcga accggtcgtc ttcacgatcg gcaagaacga cgaagccggt
     3121 gatgtcgcca agaatttgcg caagaagggg ctgattcgct ccgaacaagt cttcgagctg
     3181 acggtccgct atgtggatcg cgacattaag ccggcaacgt acgagctgac caagggcatg
     3241 tccgtctcga cgatcgtcga cctgatcacg accgagaaga gcaaggcagt caccaagctc
     3301 aaggaataca agatcaccgt cgtcgagggg tggcgaaccg agcagatcgc cgatgagctc
     3361 gacaaactca aatacccgcc gggcggcgat gccttcctgc gcgccgccga ggagtaccca
     3421 cacgactcct acgatttcct gaagggcacc aagaagggca cgctcgaggg tttcctctac
     3481 ccgg
//
LOCUS       DASSGX010000120         3274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_240136, whole genome shotgun sequence.
ACCESSION   DASSGX010000120 DASSGX010000000
VERSION     DASSGX010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240136"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VFI12_02150"
     CDS             complement(<1..618)
                     /locus_tag="VFI12_02150"
                     /inference="COORDINATES: protein motif:HMM:NF020028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055231.1"
                     /translation="MAVTEQELLTRDRVPEAETWDLTGIFVDQDALREAMGSVDRLVA
                     DVLAYRGRLAESADVLREALDAAYRARLVLERISVYARLSYDEDTTKGESQGILDEAS
                     ALAIRASQQLAWFDPELLTVPAETLNAFMADEQLAPYRHVVDDVVRNREHTRTPEIEE
                     LLAATGDISRTARDSFGALDNADLSYGQVRDEEGNLIELTKGRYHL"
     gene            842..2101
                     /gene="nadA"
                     /locus_tag="VFI12_02155"
     CDS             842..2101
                     /gene="nadA"
                     /locus_tag="VFI12_02155"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006883.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HET7055232.1"
                     /translation="MTDIRSTATIDRPAASEPLAFGESLSSWFINPQLNPANCVPEET
                     VGAYPTLRKYGASPLRQQKIPREYLKMPAEERDARIWALRNQLGDRLVILGHHYQRDE
                     VIQFADFRGDSFKLSQQAATVQNAEFTLFCGVHFMAESADILSKPGQVVILPNLEAGC
                     SMADMAKLSDVQACWDSLASIGVTGVVPITYMNSAAAIKAFCGRNGGIVCTSSNAKRV
                     YDWAFDRGEKILFFPDEHLGRNTGVAHGIPTDQMVVWDPFQPMGGCTADELRAATVIL
                     WKGYCSVHARFSVEQIEHARAAFPDVNIIVHPECTLEVVQASDYNGSTERIIDVITAA
                     PSGTVWGVGTEINLVNRLQKEMPDKTIFCLDPVICPCSTMYRIHPGYILWALEHLVEG
                     EVVNQISVDPDTRRDALVALDRMLAVP"
     gene            2167..2646
                     /locus_tag="VFI12_02160"
     CDS             2167..2646
                     /locus_tag="VFI12_02160"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HET7055233.1"
                     /translation="MAMWSKLKKAKAANTGDVAPDFDLSGTTGPGLKLSDLRGKKRAV
                     LFFYPQDCTSGCLAQLTEAGQVMDEFRALDTEPFGVNEAEAESHQVFLDQLDIPFDLL
                     VDRDFKVSRAYDALKEDQDRISRTVVVVGKNGKVIFRAAGAPPATEILREIANAKDS"
     gene            complement(2702..>3274)
                     /locus_tag="VFI12_02165"
     CDS             complement(2702..>3274)
                     /locus_tag="VFI12_02165"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET7055234.1"
                     /translation="IKQVVLRKVSAGQRDRAFTIWEEIERRIGGWARGQMTLCLVIGV
                     ISTVGYLALGLKYWVALGLWAGFTELIPFIGPILGGAAAFVVALTDSWQKALIVVAFV
                     IVLQQLESAVLVPRVMRSSVGMSPLTVILAVLVGTTLNGPLGAILAIPIGAAVQAIIQ
                     ELVRDQVDQAELEGTASTAGEPDAVQSVPT"
BASE COUNT          608 a         1092 c          991 g          583 t
ORIGIN      
        1 gaggtgatag cgccccttgg tcagctcgat caggttgccc tcttcgtccc gcacctgccc
       61 atacgacagg tcggcgttgt cgagcgcgcc gaaggaatcg cgcgcggttc gggagatgtc
      121 gccggtcgcc gccagcaact cctcgatctc gggcgtacgc gtgtgctccc ggttgcggac
      181 gacgtcgtcc acgacatggc ggtagggcgc gagctgctca tccgccataa acgcattcag
      241 cgtctctgcg gggacggtca gcagctccgg gtcgaaccac gccagttgct ggcttgccct
      301 gatcgcgagc gcactggcct cgtcgaggat cccctggctc tcgcctttgg tcgtgtcctc
      361 atcatatgag agccgcgcgt agacactgat ccgttcgagt acaagtcgcg cccggtaggc
      421 cgcatcgagc gcctcgcgca ggacatcggc cgattccgcg aggcggccgc gatacgcaag
      481 cacgtcagca acaagtcgat cgacggatcc catcgcctca cggagcgcgt cctggtctac
      541 gaatatcccg gtcaggtccc aggtttccgc ttccgggaca cgatcccggg tcaacagctc
      601 ttgctcagtt actgccacga ttcatccgcc tttcacatta cgaacggaat aatacgtgag
      661 ccgtttgggc cggcagctca ccgtcgcaca ggacttactg tcatgttgac aattactcgg
      721 tatctcgata tgatcaaatc gcgtcgggat tgggtcagaa aggctgattc cccacccgga
      781 gcagcgccgg gccgcgcatc ccgcgcccgg atcccgccgc gacgggacaa ggagaacgtg
      841 catgaccgat atccggtcaa cggctaccat cgaccgccct gctgcgagtg agccgctcgc
      901 cttcggggaa tcgctgtcat cctggttcat caatccgcaa ctcaacccag ccaactgcgt
      961 tccggaagag accgttggcg catatccgac actccgcaag tacggcgcgt cgccccttcg
     1021 tcaacagaag attccgcgcg aatatctgaa gatgccggcc gaggagcgcg atgcccggat
     1081 ttgggcgctg cgcaaccagc tcggcgaccg cctggtcatt ctcggacacc actaccagcg
     1141 ggatgaagtc atccagttcg ccgatttccg gggcgattcc ttcaagcttt cgcagcaagc
     1201 ggcgacggtg caaaacgccg agttcacgct cttctgcggc gtccatttca tggccgagag
     1261 cgccgatatc ctgtccaagc cgggtcaggt ggtgatcctg cccaatctcg aagccggttg
     1321 ttcgatggct gatatggcca agctctccga cgtccaggcg tgctgggaca gcctcgcctc
     1381 gatcggcgtc acgggtgtgg tcccgatcac ctacatgaat tcggcggcgg cgatcaaggc
     1441 cttctgtggc cgtaacggcg gaatcgtctg cacctcgtcg aacgcgaagc gagtctacga
     1501 ttgggcattc gatcggggcg agaagatcct cttcttcccc gatgagcacc tcggccgcaa
     1561 caccggcgtg gcccacggca ttccgaccga ccagatggtg gtctgggacc cgttccagcc
     1621 gatgggcggc tgcacggccg acgagctccg ggcggcgacg gtcatcctct ggaagggcta
     1681 ctgctcggtc cacgcccgct tctcggtcga gcagatcgag cacgctcgcg ccgcgttccc
     1741 ggacgtcaac atcatcgttc acccggaatg caccctcgag gtcgtccagg cgtcggacta
     1801 caacggctcg acggagcgga ttatcgacgt cattaccgct gcgccatccg gcacggtctg
     1861 gggggtcggc accgaaatca acctggtcaa ccggctccag aaggagatgc cggacaagac
     1921 gattttctgc ctcgacccgg tcatctgccc gtgctcgacg atgtaccgaa tccaccccgg
     1981 ctacatcctc tgggcgctcg agcatctggt tgagggcgaa gtcgtcaatc agatctcggt
     2041 cgacccggat acccgtcgcg atgcgctggt cgcgcttgac cggatgcttg cggtcccgta
     2101 ggttcacgga tgcgcggcgg ctcgccagcc gcgcggaatt tcgatgcaat tgcaaggagg
     2161 cggccgatgg ccatgtggag caagctcaag aaagcgaagg ccgccaacac cggcgatgtc
     2221 gcgcccgact ttgatttgtc cggcacgacc gggcccggcc tgaagttgag tgacctgcgc
     2281 ggcaagaagc gggccgtcct cttcttctac ccgcaagact gcaccagcgg ctgcctggcc
     2341 cagctcaccg aggccgggca ggtcatggat gagttccggg cgctcgacac cgagccattc
     2401 ggtgtcaacg aagccgaagc cgagagccac caggtcttcc tcgaccagct cgacatcccc
     2461 tttgacctgc ttgtggaccg cgacttcaag gtctcccgcg cctacgatgc gctgaaagag
     2521 gaccaggacc gtatctcgcg gaccgtcgtg gtcgtcggca aaaatggcaa ggtcatcttc
     2581 cgggcagccg gcgcgccgcc ggcaaccgag atcctgcgcg agatcgctaa cgccaaagac
     2641 agctaaccga atctcgacgt caaatcaaaa tgccgcgcgg tgacgtccgg cattttgcat
     2701 gtcaggtagg cacgctctga accgcatccg gttctccggc ggtggacgcc gtgccctcaa
     2761 gctcggcctg atccacctgg tcgcggacca gctcctggat gatcgcctgg accgcggcgc
     2821 cgatcggtat cgccaggatc gcgccgagtg gcccgttcag tgtcgtgccc acgaggacgg
     2881 ccaggatgac ggtcagcggc gacatgccga ccgagcttcg catgacgcgg ggcaccagga
     2941 cggcactttc gagttgctgg agcacgatca cgaatgcgac cacgatcagg gccttctgcc
     3001 aggaatcggt cagcgccacc acgaaggccg ccgcgccgcc gaggatcggt ccaatgaacg
     3061 ggatcaactc ggtgaagccg gcccagaggc cgagcgcaac ccagtatttc aacccgagcg
     3121 ccaggtaccc gaccgtcgag atcacgccga tgaccaggca gagcgtcatc tggccccgcg
     3181 cccaaccgcc gatccgccgc tcgatctctt cccagatcgt gaatgcccgg tcccgctggc
     3241 ccgctgagac cttgcggagg acaacctgct tgat
//
LOCUS       DASSGX010000121         2904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1218361, whole genome shotgun sequence.
ACCESSION   DASSGX010000121 DASSGX010000000
VERSION     DASSGX010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218361"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VFI12_02170"
     CDS             complement(<1..329)
                     /locus_tag="VFI12_02170"
                     /inference="COORDINATES: protein motif:HMM:NF013161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET7055235.1"
                     /translation="MAGTAVLERLSWRLADVVGTIDETPHAKTLILRVPGWRGHRPGQ
                     HVDVRLTAEDGYQAERSYSIASAPNGERVELTIEELEDGEVSSYLTEVLVNGDQLELR
                     GPIGGYFT"
     gene            complement(299..907)
                     /locus_tag="VFI12_02175"
     CDS             complement(299..907)
                     /locus_tag="VFI12_02175"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HET7055236.1"
                     /translation="MSGFVSRGFRGRRREPEVAGRLPPGQYLTRDFPVLSAGPTPRTP
                     HDRWDFRITGAVDQERHWTWEQFLALRRETPIVDIHCVTKWSKLDTVWEGVSIDTLLA
                     GLHHTARFVIAHCDGGYTTNLPLEDVTGGKAWVAFGYGGEPLDPEHGGPARLLVPHLY
                     FWKSAKWIRGLELLPEDEPGFWESAGYHNYGDPWREQRYWND"
     gene            1103..2350
                     /locus_tag="VFI12_02180"
     CDS             1103..2350
                     /locus_tag="VFI12_02180"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7055237.1"
                     /translation="MLALASLAVFLSGPAQTYGVSVFIDPILADFGWSRSLISTLYSI
                     ATLAGAIPMILIGRQIDRVGNRLIMTIAALLFGLSLLWLSVVSGPIFVLLGLAILRTF
                     GSGVLTLGARTLVPHWYVRRRGWAFSMLGISGAISLAVVPRFNEALISWVGWRHAWQI
                     IAAITLVGLTPVLGALIRNRPEDVGEKPDGHHAGRRLRRPSLLVDAEDDWTVREAMTT
                     RMFWAMLLAGTVPAVVLTGLSFHQTSLLTARDLSPGLAASVFAAESMVALPITLAAGW
                     VVDRYPIRYTLATAQVALLVALVALLAADSVPLALLYGGLRGVSTGLWAVSADVAWTS
                     FFGKKYLGSIRGVTFAVGTVGAAIGPVPLGLVYDATSSYSGAIIAYMVLPLIALAVLM
                     AAGIPRKEVASSQMSVASPVTHM"
     gene            2486..>2904
                     /locus_tag="VFI12_02185"
     CDS             2486..>2904
                     /locus_tag="VFI12_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055238.1"
                     /translation="MKTRQYLVRTLTLVAFAVTLGLANLDHAGAADNDPSQNATTEIQ
                     VCEAMGGHAEVTIDRTAGRLIVIVECKGGAMDGFHCINDAGFTDCAFARFDLDDTISV
                     AEPGGIKPLDDGPRAPIEAVEDASDVQELPATPTPEP"
BASE COUNT          453 a         1029 c          897 g          525 t
ORIGIN      
        1 gtaaagtagc cgccgatcgg cccgcgcagc tcgagctggt cgccattcac gagcacctcg
       61 gtcagatagg acgaaacctc gccgtcctcc agctcctcga tggtcagctc gacccgctcg
      121 ccattcggcg cggaggcgat cgagtagctc cgctcggcct ggtagccgtc ctccgcggtc
      181 aggcggacat cgacgtgctg accaggacga tgcccgcgcc accccggcac gcgcaggatg
      241 agcgtcttcg cgtgcggcgt ctcgtcgatt gtcccgacaa cgtcggcgag gcgccagctc
      301 agtcgttcca gtaccgctgt tcccgccatg gatcaccata gttgtgatat ccggccgatt
      361 cccaaaagcc cggctcgtcc tcaggcaaca actcgaggcc gcgaatccac ttggcgctct
      421 tccagaaata gaggtgcggc accagcaacc gggccggacc gccatgctcc gggtcgagcg
      481 gctcgccgcc gtatccgaat gccacccacg ccttgccacc ggtcacatcc tcgagcggca
      541 gattggtcgt gtacccgccg tcgcaatggg cgatcacgaa gcgggcggtg tggtgtaatc
      601 cggccaagag cgtatcgatc gagacgccct cccagaccgt atccagtttc gaccacttgg
      661 tgacgcagtg gatatcgacg atcggcgtct cgcgccgcag ggcgaggaac tgctcccatg
      721 tccagtgccg ctcttggtcg accgcgccgg tgatccggaa atcccagcgg tcgtgcggcg
      781 tgcgcggcgt tggcccggcc gagagcaccg ggaagtcgcg cgtcagatac tgccccggtg
      841 gcagtcgccc ggccacctcc ggctcgcgcc gtcgccccct gaacccgcgc gaaacaaacc
      901 cactcatagc gcattgcctc cacgaccatt atcagccgtg agacgggagt cgtgagaacg
      961 acacccaact cacggctcac ggctcacgcc tcctcggctc cgttatcatc acgcaacgcg
     1021 agatcgccac cgtgcactga agagggcgct aatccggatg attcgccgcc gaccgagtga
     1081 ggttccgttt cggggcggtt tgatgttggc gctggcctcg ctcgccgtct tcctctccgg
     1141 tcccgcccag acctacggcg tctccgtctt catcgacccg attctggccg atttcggctg
     1201 gtcccgctcc ctgatctcga ccctctattc gatcgccact ctggccgggg cgatcccgat
     1261 gatcctgatc ggccgccaga tcgaccgcgt cggcaatcgc ctgatcatga cgatcgccgc
     1321 gctgctcttc ggcctctccc tgctctggct gagcgtcgtc tccggtccga tcttcgtcct
     1381 gctcggcctg gcgatcctgc gcacctttgg ttccggcgtc ctgacgcttg gcgcgcggac
     1441 gctcgtgccg cactggtacg tgcgccggcg gggctgggcc ttcagcatgc tcggcatctc
     1501 cggggcgatc agcctcgcgg tcgtgccccg cttcaatgag gcgctgatca gctgggtcgg
     1561 ctggcgtcat gcctggcaga tcatcgcggc gatcaccctg gtcggcctga ccccggtgct
     1621 gggcgccctg atccggaacc ggccggaaga tgtcggcgag aaaccggacg gccatcacgc
     1681 cggcaggcgg cttcggcgac catccctgct ggtcgatgcc gaggacgact ggaccgtgcg
     1741 cgaggcgatg actacgcgca tgttctgggc gatgcttctc gccggaacgg tccccgctgt
     1801 cgtcctgacc ggactcagct tccaccagac gtcgctcttg accgcccggg atctctcgcc
     1861 cggcctggcc gccagcgtct tcgccgccga atcgatggtc gccctgccga ttacgctcgc
     1921 cgccggctgg gtcgttgatc gctacccgat ccgctacacg ctggccaccg cgcaagtggc
     1981 attgctggtc gcgctcgtcg ccttgctggc cgccgacagc gtcccgctcg ccctgctcta
     2041 cggcgggctg cgcggcgtct cgaccgggct ctgggcggtc tcggccgatg tcgcctggac
     2101 ctcgttcttc ggcaagaagt acctgggctc gattcgcggc gtcaccttcg cggtcggcac
     2161 cgtcggcgcg gccatcggcc ccgtcccgct cggcctcgtt tacgacgcga ccagctccta
     2221 cagcggcgcg atcatcgcgt acatggtcct gccgctgatc gctctcgccg tcctcatggc
     2281 cgccggcatc ccgcgcaaag aagtagccag tagtcagatg tcagtagcca gtccggtcac
     2341 gcatatgtga ccaccggcta cgagctactg gctactggct actggagtct cagttacgtg
     2401 atctcaccga tgctcgcgag cgacgctccg gcgatcatcg atcccaggca aggacctgat
     2461 gatcgatgag agagggagca cgaagatgaa gacgcggcag tacctggtac ggaccctgac
     2521 cctggtggcg ttcgccgtca ccctcggctt ggcgaatctc gatcacgccg gagcggcgga
     2581 caacgacccg tcacaaaacg ccacgaccga gattcaggtc tgcgaggcga tgggcggtca
     2641 cgcggaggtc acgatcgacc gcaccgccgg tcgacttatc gtcattgtcg aatgcaaggg
     2701 cggcgcaatg gacggcttcc actgcatcaa cgacgcgggc ttcaccgact gcgcgttcgc
     2761 gcgcttcgac ctggacgaca ccatctcggt ggcggagcct ggtggcatca agccgttgga
     2821 cgatggtccc cgcgcaccta ttgaagcggt cgaggatgcg tccgatgtgc aggaactgcc
     2881 agccacgccg acgcccgagc cgag
//
LOCUS       DASSGX010000122         2118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_22931, whole genome shotgun sequence.
ACCESSION   DASSGX010000122 DASSGX010000000
VERSION     DASSGX010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22931"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1364
                     /locus_tag="VFI12_02190"
     CDS             <1..1364
                     /locus_tag="VFI12_02190"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HET7055239.1"
                     /translation="KTSLGKAIAQAIGRKFVRVSLGGVRDEAEIRGHRRTYIGAMPGR
                     LITAMKQAGTVNPVILLDEIDKLSSDYRGDPASALLEVLDPAQNKNFVDHFLDVAYDL
                     SNVLFITTANYLGQIPRPLRDRMEIIEVGGYTEDEKAEIGRRYLLARNMTAHGIPDGS
                     LEVPHKVWIDLIRDYTREAGVRELDRSVAALCRKTAREIVRGKAGKVRITKSKLEELM
                     GPKHYGLEMVTGQEQIGTAMGLGTTEVGGELIPVEVLTMPGKGGLTITGRAGEVMQES
                     AQAALSYARSRTHQLRIDPGFRERLDLHIHLPEGAQPKDGPSAGITMATALISALTRR
                     PVRGDTAMTGEITLRGRVLPIGGLKDKTLAAHRCGIRRMIAPAQNRREFEKLPANVAK
                     EMTFIWVSDMDEVIRAAILLDDDQVGNLANAGEDIPADSIAPAGGFGTHVVTDAQPRQ
                     TES"
     gene            complement(1349..>2118)
                     /locus_tag="VFI12_02195"
     CDS             complement(1349..>2118)
                     /locus_tag="VFI12_02195"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HET7055240.1"
                     /translation="ADELANAVLAADQERKERSGQLISEVREALAGDKALTPVIVVHG
                     ETWASGLLGPVAAKIAEEHQRPAVVLGQSGEFLAGSGRSFGAWDMAEAFRQVDFLVRH
                     GGHAKAAGLTVEKKLIADLRERLCELYERSEIEVAPEPEFIIEADIDADPVTLGLAEK
                     IEAIGPFGAGNERPILRMRGVQITQWKPIGKDKAHAHVWLSRGGGSLRAVWFNGAERL
                     RELDTSQRINVLVSLSINVWNQTRSVDARLIDLQLSV"
BASE COUNT          388 a          754 c          653 g          323 t
ORIGIN      
        1 gcaagaccag cctcggcaag gcgattgccc aggcgatcgg gcgcaaattc gtgcgcgtct
       61 cgctcggcgg cgtccgcgac gaggccgaga tccggggcca tcgccgcacc tacatcggcg
      121 cgatgcccgg ccgattgatc acggccatga agcaggccgg cacggtcaac ccggtcatcc
      181 tgctcgacga aatcgacaag ctctcgtccg attaccgcgg cgacccggcg tcggctctcc
      241 tcgaagtgct ggacccggct cagaacaaga acttcgtcga ccacttcctc gatgtcgcct
      301 acgacctctc gaatgtcctg ttcatcacga ccgccaacta cctcgggcaa attccgcggc
      361 ccctccgaga ccggatggag atcatcgagg tcggcggcta caccgaggac gagaaggccg
      421 agatcgggcg gcgctacctg ctcgctcgca acatgaccgc gcacggcatc cccgatggct
      481 cgctcgaagt tccgcacaag gtctggatcg acctgatccg ggactacacc cgcgaagccg
      541 gcgtccgcga gctggatcgc tcggtcgcag ccctgtgccg caagaccgcg cgcgagatcg
      601 tccgcggcaa ggcgggcaag gtccggatca ccaagagcaa gctcgaggag ctgatgggac
      661 cgaagcatta cggcctggag atggttaccg gccaggagca gatcggcacc gcgatgggcc
      721 tcggcacgac tgaagtcggc ggcgagctca tcccggtcga agttctgacg atgccgggca
      781 agggtggact gaccatcacc ggcagagccg gcgaggtgat gcaggagtcg gcccaggccg
      841 cgctctccta cgcccggtcg cgcacgcacc aactccgcat cgatcccggc ttccgggagc
      901 ggctggactt gcacatccac ctgccggaag gcgcccaacc caaggacggc ccgtcagcgg
      961 gcattaccat ggctaccgct ctcatctcag cgctcaccag gcggcctgtg cggggcgata
     1021 cggccatgac cggagagatc accctccgcg gccgcgtcct gcccatcggc ggcctcaagg
     1081 acaagaccct cgccgcccac cgctgcggta tccggcgaat gatcgccccg gcccagaacc
     1141 ggcgcgaatt cgagaagctg ccggcgaacg tggccaagga gatgacgttc atctgggtga
     1201 gcgatatgga cgaggtgatc cgcgccgcga tcctgctcga cgacgaccag gtcggcaacc
     1261 tcgcaaatgc cggagaagat atcccggccg actcaatcgc gccggcgggc ggattcggca
     1321 cgcacgtcgt gaccgacgcc cagccgcgtc agaccgagag ctgaaggtca atcaacctgg
     1381 cgtcaacgct ccgggtctga ttccagacgt tgatcgagag cgagaccagc acattgatcc
     1441 gttggcttgt gtccagctcg cgcagccgct ccgcgccatt gaaccagacg gcgcggagcg
     1501 atccccctcc ccggctgagc caaacgtgcg cgtgggcctt gtccttgcca atcggcttcc
     1561 actgcgtgat ctgaacaccc cgcatccgca gaatcggccg ctcattgccg gcgccaaatg
     1621 gaccgatcgc ctcgatcttc tcggccagcc caagcgtgac cgggtcggcg tcgatgtcgg
     1681 cttcgatgat gaattccggc tccggcgcga cctcgatttc tgatcgctcg tagagctcgc
     1741 acaagcgctc ccgcagatcc gcgatcagct tcttttccac ggtcagacca gccgccttgg
     1801 cgtggccgcc gtggcgcacc aggaaatcga cctgccgaaa cgcctcggcc atgtcccacg
     1861 ccccgaagga acgtccggaa cccgccagga actctccgga ctggccgagc acgacggccg
     1921 gccgctggtg ctcctcggcg atcttggcag ccaccggacc aagcaggccg gatgcccagg
     1981 tctctccgtg gaccacgatg accggcgtca gcgccttatc gccggccagc gcctcgcgaa
     2041 cctcgctgat gagctgcccg gaccgttcct tgcgttcctg atcggccgcg agcaccgcgt
     2101 tcgcgagctc atccgccg
//
LOCUS       DASSGX010000123         2396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_131687, whole genome shotgun sequence.
ACCESSION   DASSGX010000123 DASSGX010000000
VERSION     DASSGX010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131687"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1336
                     /locus_tag="VFI12_02200"
     CDS             <1..1336
                     /locus_tag="VFI12_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055241.1"
                     /translation="VGLFTTLFTHWYGIFSSTFATDGTLLYWLGQHDVRRGEQPWFYF
                     LLLLPQYEFLPITIGAALGLLATWRAVRTWMTGEVQDERLFFRLFLAFWFFGIFAILS
                     WAGEKMPWLIVHIALPGTILAGAAVGRLIERGIALAETRRIGLLDLGIFAGLIAAVIG
                     WFFLAARLTYGTFNGPCANGDTDCRRVTDSDLNKWWLLAIPPLVWLGFMLVAGLRRGW
                     RQSALIAVSAVVVVLALLEVRVGWRLSYTNPDVPTEMMVYTQTSVDVKRAVLESNQLS
                     RELAPQGQGQILFDTGADGLSWPLYWYFRDNPNATPFGGTLPSNTDAAVIFILSSRSG
                     TPQNQQVLANYTGTSYAFRWHFPEELYRNFAIAPELRPGRSAWANASDPHGPLEVLKS
                     VVESSASIFDAHGQQELFRMVVYRDIDDVLGYYEFQVYVRTDLLPEFNQIRY"
     gene            1364..>2396
                     /locus_tag="VFI12_02205"
     CDS             1364..>2396
                     /locus_tag="VFI12_02205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET7055242.1"
                     /translation="MAELSAEQRPDLFPRRGLSATINLAQITWLTVAWAAVMVAGAGL
                     RLFQLGAESLSPTEAQKAFDAWSLVRGATEGPYREPSHTSPTGLLLRSFSFFLFGDSD
                     TTTRVVSALLGIGLIALIWSIRDLLGDLRALGAAALVALSPTMVFESRIVNEEIVSLF
                     FGFLLVVTVFRIGAVDRIPGIGRAICLGFAAAGLIGSGPAGITVLLTLVIMFAVSPLI
                     SLDHGATVRRAVRRVLDTRNLLAGGVISFVIVLGLLFTRFFSDPGAISGLGDVFADWG
                     KLIATASSDTPTQFFILSLLMYEIVGVAFAVKAAVTEPAEDESVARIDWSYPAVWFLA
                     SLVIFSFSSG"
BASE COUNT          329 a          820 c          752 g          495 t
ORIGIN      
        1 cgtcggcctg ttcacgacgc tcttcacgca ctggtatggg atcttctcgt cgaccttcgc
       61 gaccgacggc acgctccttt actggctcgg ccagcacgac gtccgccgcg gcgagcagcc
      121 gtggttctat ttcctgctgc tcctgccgca gtacgagttc ctgccgatta cgatcggcgc
      181 cgcgctcggt ctgcttgcga cctggcgcgc ggtgcggacc tggatgaccg gcgaagttca
      241 ggacgagcgg ctcttcttcc gcctcttcct cgccttctgg ttcttcggca tcttcgccat
      301 cctctcctgg gccggcgaga agatgccctg gttgatcgtc cacatcgccc tgccggggac
      361 gatcctggcc ggcgcggcag ttgggcggct gatcgagcgg ggtattgccc tcgccgaaac
      421 acggcggatc gggttgctcg atctggggat cttcgcgggg ctgatcgcgg ccgtgatcgg
      481 ctggttcttt ctcgccgcgc ggctgaccta tggcaccttc aacggtccgt gcgccaacgg
      541 cgacaccgat tgccggcggg tgaccgacag cgacctgaac aagtggtggc tcctggcgat
      601 accgcccctg gtctggctcg ggttcatgct cgtcgcaggg ctccggcgcg gatggcggca
      661 gtcggcgctg atcgcggtca gcgcggtcgt cgttgtcctc gcgctgctcg aggtccgggt
      721 cggctggcgg ctcagttaca ccaacccgga cgtcccgacc gagatgatgg tctacacgca
      781 gacgtcggtc gacgtgaagc gggccgtcct cgaatcgaac cagctcagtc gcgagctcgc
      841 gccccagggt cagggacaga tcctgttcga caccggtgcg gacggcctct cctggccgct
      901 gtactggtac ttccgcgaca acccgaatgc gaccccattc ggtgggacgc tgccctcaaa
      961 cacggacgcc gcggtcatct tcatcctctc gagccgatcg ggcacgccac agaatcagca
     1021 ggtgctcgcg aattacaccg ggaccagcta cgccttccgc tggcatttcc ccgaggagct
     1081 ctaccggaac ttcgcgatcg cgccggagct gcgccctggc cggtccgcct gggcgaacgc
     1141 gagcgacccg catggtccac tcgaggtcct caagtcagtc gtcgagagct cggcctcgat
     1201 cttcgatgcg catggccagc aggagctgtt ccggatggtc gtctaccgcg acatcgatga
     1261 cgttctcggt tattacgagt tccaggttta cgttcgcact gatctcctgc cggagttcaa
     1321 ccagattcgg tactagccac cagcaaggag ttccaccaca ccaatggcag agctttcggc
     1381 agagcagcgg cccgacctgt ttccacgtcg tggcctcagc gcgacgatca accttgccca
     1441 aataacctgg ctgaccgtcg cctgggccgc ggttatggtc gccggggccg gactgcgcct
     1501 gtttcagctc ggggcggaat cgctttcgcc gaccgaagct cagaaggcgt tcgacgcctg
     1561 gtcgctggtc cgtggcgcga ccgaggggcc gtaccgcgag ccgtcccaca catcgccgac
     1621 cggtctcctg ctgcgctcgt tctccttctt cctgttcggc gattcggata cgaccacgcg
     1681 cgtcgtctcg gccctgctcg ggatcggtct gatcgcgctg atctggtcca tccgcgacct
     1741 gctcggggat ttgcgcgcgc tcggcgcggc ggcgctggtg gcgctctcgc cgacgatggt
     1801 ctttgagagc cggatcgtca acgaggagat cgtcagcctc ttcttcggct tcctgctcgt
     1861 cgtcacggtc ttccgcatcg gcgccgtcga ccgcatcccc gggatcggcc gcgcaatctg
     1921 tctcggcttc gccgcagccg gtttgatcgg ctccggtccg gctgggatca ccgttctgct
     1981 cacgctggtc atcatgtttg cggtttcgcc cctgatcagc ctcgaccacg gagcgaccgt
     2041 gcggcgcgcg gttcggcgcg tgctggatac ccgcaacctg ctcgccggcg gggtgatctc
     2101 cttcgtaatc gtcctgggcc tgctgttcac gcgcttcttc agcgatcccg gcgcgatctc
     2161 cggactcggt gatgtcttcg ccgactgggg caagttgatc gcgactgcgt cgagcgacac
     2221 gccgacccag ttcttcatcc tctcgctcct gatgtacgaa atcgtcggcg tggccttcgc
     2281 ggtcaaggcc gccgtgaccg agccggctga ggacgaaagc gtggcccgga tcgactggtc
     2341 gtacccggcg gtctggttcc tggcctcgct cgtaatcttc agcttcagct cgggcc
//
LOCUS       DASSGX010000124         4277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1438056, whole genome shotgun sequence.
ACCESSION   DASSGX010000124 DASSGX010000000
VERSION     DASSGX010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1438056"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VFI12_02210"
     CDS             complement(<1..209)
                     /locus_tag="VFI12_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055243.1"
                     /translation="MASTAVELQTARGHGAVGHGEHGHTGGHHTSMGIDSRKVGMWVF
                     LASDCMFFGSLIATYLIYRGRAGEL"
     gene            complement(228..2174)
                     /gene="ctaD"
                     /locus_tag="VFI12_02215"
     CDS             complement(228..2174)
                     /gene="ctaD"
                     /locus_tag="VFI12_02215"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HET7055244.1"
                     /translation="MASIAQPAPATATYAGSGLKPQSGVWSWLTTIDHKRIGILYGVT
                     ALFLFLVGGLEALVIRVQLSHPNNDVVSANTYNELFTMHGTTMIFLALMPLTAALFNY
                     LVPLQIGARDVAFPRLNAFSYWAFLFGAIILNMSWFFNEAPNQGWYGYAPLTEKAWNP
                     GRHVDFWMLGLQVLGVASVAAGINMIVTIFNLRAPGMSMMRMPIFTWSILVTQVLIVM
                     AFPAFTIALILLTMDRYAGTGFFEIAAGGDPLLWQHLFWVFGHPEVYILVLPAFGIVS
                     EIIPVFSRKPLFGYSVMVYALAAIAFLGFGVWAHHMFTTGLGPTANAVFAGSTMLIAI
                     PTGVKIFNWLGTMVGGSLKFTTPMLFAIGLVSQFTIGGLSGVMHGSPPVDSHHNDTYF
                     VIAHFHYVLFGGTAFGLLAAAYYWFPKMSGRMMNDRLGKINFWTTFIGFNATFFPMHF
                     LGLEGMPRRYYTYGSGSGWELWNVVVSCGALFLGASMLLFVYNLGYSLKHGEIAGPNP
                     WDAATLEWAIPSPPPAYNFANLPVVGHRDPLWWEKYDDHAHAGHRRDPQDLTLVNQVT
                     GHIHMPNPSIYPLITAFGLFLGAIGMLFSDPRITIGLFHLPSVSLLGIIILVGGIYGW
                     AFEPASDPEPEPAHIESTTTVVSH"
     gene            complement(2221..3507)
                     /gene="coxB"
                     /locus_tag="VFI12_02220"
     CDS             complement(2221..3507)
                     /gene="coxB"
                     /locus_tag="VFI12_02220"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HET7055245.1"
                     /translation="MRGRERQRAPIQKSTRNRLVVLLSVALLGVMTLLPRLAAADDVY
                     TTISPESRDARDIQFLYKLIFWLALIVFVGVQAAIVYSVLRYRRRYENEPRPEQIHGN
                     QKLEIIWTIIPAIVLVAIFIPTVRTMYRIDNRANEGQYTIQVYGKQWWWEVHYTEPDT
                     VAGVVTANEFYIPVNTRVRIELLSSNVIHSFYVPQLAGKMDVMPGHTNVMSVEADKPG
                     MYYGECAEYCGEQHAFMRFKIIAVPQDQFDVFINGWKEGASSAAASLVPEGDVDRVPQ
                     AMSLCLGCHRIGGVPNNAAGTPLENPPMGVEGGTLENQILGPNLSMFACRTTVAAGIL
                     PNTAEGVHTWLDNPGGVKPGNWMATMIKSDTLSEDQINQIVAYLETLHPSQGCPQITG
                     VNADRVERLVDDPSAQQTADESTADEARVNREHNKS"
     gene            3936..4163
                     /locus_tag="VFI12_02225"
     CDS             3936..4163
                     /locus_tag="VFI12_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055246.1"
                     /translation="MSRQGGTRWLPAVLIVIGALLVIGSIFADSLGFSWGGEGYGWKQ
                     MLATIVGLVIALGGVGLLMRPPTPPRVRPRP"
BASE COUNT          846 a         1265 c         1413 g          753 t
ORIGIN      
        1 tagagctcgc cggcccggcc gcgatagatc agataggtcg cgatcagcga tccaaagaac
       61 atgcagtccg atgccaggaa gacccacatc ccgaccttgc gggagtcgat gcccatgctg
      121 gtgtggtggc caccggtgtg accatgctcc ccatgcccaa ccgcgccgtg gccgcgggca
      181 gtctggagtt cgacggctgt cgatgccatt ccgtgaaacc tttccgccta gtggctgacg
      241 accgtggtgg tcgattcgat gtgcgccggt tcgggctccg gatcagatgc cggctcgaag
      301 gcccagccgt agatcccccc gacaaggatg atgatgccga gcaggctgac cgacggcagg
      361 tggaacaagc cgatcgtgat gcgcggatcg gagaagagca tgccaatcgc gcccaggaac
      421 aggccgaagg cggtgatgag cgggtagatc gacggattgg gcatgtgaat atggccagtc
      481 acttgattaa caagcgtcag gtcctgcgga tcgcgtcgat gccctgcgtg cgcatggtcg
      541 tcgtacttct cccaccagag cgggtcgcga tggccgacga ccggcaagtt cgcaaagttg
      601 taggccggcg gcggcgaggg gatggcccac tcgagcgtcg cggcgtccca cgggtttggg
      661 ccggcgattt cgccgtgttt gaggctatag ccgaggttgt agacgaagag cagcatcgag
      721 gcgccgagga acagtgcacc gcagctgacg acgacgttcc agagctccca gccactgccc
      781 gaaccgtatg tgtaatagcg gcgtggcatg ccctcgaggc caaggaagtg catcgggaaa
      841 aaggtcgcat tgaagccgat gaacgtggtc cagaagttga tcttgccgag acggtcattc
      901 atcatccggc cgctcatctt cgggaaccag tagtaggccg ccgcgagcaa accaaatgct
      961 gttccaccga acaatacata gtggaagtgc gcgatcacga agtaggtatc gttgtggtgg
     1021 ctgtccaccg gcggcgaacc gtgcataacg cccgagagtc caccgatcgt gaattgcgag
     1081 acgagaccga tcgcgaagag catcggcgtc gtgaacttga gtgacccgcc caccatggtg
     1141 ccgagccagt tgaagatctt cacgccggtc gggatcgcga tcagcatcgt ggagcccgcg
     1201 aatacggcgt tcgccgtcgg gccgagaccg gtcgtgaaca tgtggtgcgc ccagacgccg
     1261 aatcccagaa acgcgatcgc ggcgagggcg tagaccatga ccgagtagcc gaagagcggc
     1321 ttccgggaaa agaccgggat gatctcggag acgatgccaa aggccggcag cacgaggata
     1381 tagacctcgg gatggccaaa gacccagaag aggtgctgcc agaggagtgg atcaccgccg
     1441 gcggcaatct caaagaagcc ggtgcccgca taccggtcca tcgtcagcag gatcagggcg
     1501 atcgtgaacg ccgggaacgc cataacgatc aggacctgcg tgaccaggat ggaccaggtg
     1561 aagatcggca tccgcatcat ggacatgccg ggcgcccgca agttgaagat ggtaacgatc
     1621 atattgatgc cagccgcgac cgaggcgacg ccgagtacct gcaggccgag catccagaag
     1681 tcgacgtgcc ggcccggatt ccaggccttt tcagttagcg gggcgtagcc gtaccagccc
     1741 tgattcggcg cttcattgaa gaaccagctc atgttgagga tgatcgcgcc gaacaggaag
     1801 gcccagtaac tgaaggcgtt cagccgcggg aaggcgacgt cccgggcacc gatctgaagc
     1861 ggcaccaggt agttgaacaa cgcggcggtc agcggcatca gcgcgaggaa gatcatcgtc
     1921 gtgccgtgca tcgtgaagag ctcgttgtag gtgttggcac tgacgacatc gttattcgga
     1981 tgagagagct ggacgcggat gaccagcgcc tcgagaccgc caacgaggaa caggaagagg
     2041 gccgtcaccc cgtagaggat gccgatgcgc ttgtggtcga tcgtggtgag ccagctccag
     2101 accccggact ggggcttgag gccggacccc gcgtatgtcg ctgtggctgg cgccggttgg
     2161 gcaatactgg ccattaccgc tcctccgcgt tggggcgcaa tccgtggttc gatgctgttg
     2221 tcatgacttg ttgtgctccc ggttgacgcg ggcttcgtct gccgttgact catcggctgt
     2281 ctgctgcgcg ctcgggtcgt caacgaggcg ctccacccgg tcggcgttca cgccggtgat
     2341 ctgcgggcag ccctgcgagg gatggagcgt ttcgaggtag gccacgatct ggttgatctg
     2401 gtcttcggag agcgtgtcgc tcttgatcat ggtcgccatc cagttgcccg gcttgacgcc
     2461 gcctggattg tcgagccagg tatggacgcc ctcggccgtg ttcggcagga tgccggcggc
     2521 gacggtcgtc cggcaggcga acatgctcag gttcgggccg aggatctggt tctcaagcgt
     2581 gccgccctcg acgcccatcg gcggattctc caatggagtg ccagccgcgt tgttaggcac
     2641 gccgccgatc cggtgacagc cgaggcagag cgacatcgcc tgtgggacgc ggtcgacatc
     2701 accttcgggc acgagggagg cagcggccga actcgcgccc tccttccagc cgttgatgaa
     2761 gacgtcgaac tggtcttgcg gcacggcgat gatcttaaag cgcatgaagg cgtgctgctc
     2821 gccgcagtac tccgcgcatt cgccgtaata catgcccggc ttgtcggcct cgacggacat
     2881 cacgttcgta tgccccggca tgacgtccat cttgccagcg agctggggca catagaagga
     2941 atggatgacg ttcgatgaca acaactcgat acggacgcgg gtgtttaccg ggatatagaa
     3001 ctcgttcgca gtcacgaccc cggcgacggt gtccggctcc gtgtagtgga cttcccacca
     3061 ccactgcttg ccatagacct ggatcgtgta ctggccctcg ttggcacgat tatcgatccg
     3121 gtacatcgtg cggaccgtcg ggatgaagat cgccaccagc acgatggccg ggatgatggt
     3181 ccagatgatt tccagtttct ggttgccgtg aatctgctcc ggccggggct cgttctcgta
     3241 ccggcggcga tagcgcagca ccgagtagac gatcgcggcc tgcacgccga cgaagacgat
     3301 cagcgcgagc cagaaaatca gcttgtaaag gaactggatg tcgcgggcgt ccctcgactc
     3361 gggggagatc gtcgtataga cgtcatctgc ggcggcgagg cgcggcagga gcgtcatgac
     3421 tccaagcagc gccaccgaca acaacacgac aagtctgttc cgggtcgatt tctgaatggg
     3481 tgctcgttgt cgctcccgac ccctcatgct gcctcctgct cgcaccgaaa ccctcgcatc
     3541 tcgcacagaa aaaacgaaac acgcaccatg ccgccaaccc cgggcggggg tggcgaatgc
     3601 agtgcgtggt gtcgtgccat gccgagcata tctgttccaa tcgcatgtga gatccgggca
     3661 ttaccccgac ctcccgcgac cggccccgct ccccaacgag tgccacagag acgcaaaggc
     3721 gcggacagac cgcgcagcgt ccacctcatg tcgcgcatcg tacacgattg ctgactgatt
     3781 cggtcaagat cgcgtgatac ggccttgggc atgagtgcga aagtctggaa actggactac
     3841 catcgttcat gaatcgatcg gtggcggacc cgggcgagcg ccgcatgcgg ctgcgcccgg
     3901 atcgccgaca gtgcggggca gagatgcaac ctcagatgtc acggcaaggc gggacccggt
     3961 ggctgcccgc ggtgctgatc gtgatcggcg cactgctggt gatcggctcc atctttgcgg
     4021 attcgctcgg attcagctgg ggtggcgagg ggtacggctg gaagcagatg ctggcgacga
     4081 tcgtcgggct ggtgatcgcg ctcggcggcg tgggcctcct gatgcgaccg ccaacgccgc
     4141 cccgggtgcg cccacgaccg taaatattcg gatgcgggac gctcggggct caatggttgt
     4201 tgaggcacaa aaccggtagt cggaacccgg gaacggagcg cggggttacc acgccccgca
     4261 atcgcccgca gggcgat
//
LOCUS       DASSGX010000125         2977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_241021, whole genome shotgun sequence.
ACCESSION   DASSGX010000125 DASSGX010000000
VERSION     DASSGX010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241021"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2977)
                     /locus_tag="VFI12_02230"
     CDS             complement(<1..>2977)
                     /locus_tag="VFI12_02230"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7055247.1"
                     /translation="LLELTESDLEKLGVLLGHRKRILRAVGVLKETGGAAQAAAPSKR
                     AAVAARPERAERRQVTIMFCDLVGSTPLSSRLDPEDLRQVLGMYNGLVSEVTREFDGT
                     VNRYMGDGVLICFGHPQAYEDDAERAVHAGLALIDRIGKMEFPCGPLEIRIGIATGPV
                     VIGDLIGTGDAQERGIVGETPNLAFRLQGLAPPNSLLINSITRQLIGDFFEYRDVGGL
                     DIRGFARRVPVWQVLRSSGLASRFEALRAASMTPLVGRHEELALLRQLWERVTAGEGQ
                     IVQLSGEPGIGKSRIIAAIQEQLGNEGYIRLRYFCSPHHVGSTLYPFIAHLEHAARFA
                     REDTPEVKFEKLARLLTNSAAGDEAVILFGDLLGLPTGDGNPAASMDPQRRRERLLAA
                     AVRHLEGLAHEQPVLLICEDMHWIDSSSLELLVMLAKRVPHIPVLIVLTFRPEFQPPW
                     VDWSNVTTLALSRLSRHDAAMLAARVAGGSNTFRPEIIDQIVDRTDGVPLFIEELTKS
                     LLENSLLGRLPDARGRNGSVPQLAIPTSLQASLMARLDRLSPAGRDVAQIGAALGREF
                     SYEILEALTPDRTEHLQEAIDQLASAGLVFWRGTPPRASIMFKHALIQDAAYSTLLRA
                     HRQELHALIAKTLESRFPETSDLEPEILAHHYSQAGLFDEAIEYWRKAGERALRRSAN
                     VEAMAHLKKGIELIHLLPTAPERDRRELRLYLALGPAIRAIQGHATDEVLEVYSRARA
                     LLHENAGAREQMGVLYGLWITRFTRWDDAATGELAEQMIELSHQHQEVEFSILANALM
                     GNTLWARGAFVPARDHLERSLSLCEVDDKRSLHNHSIAALSFLGVTLWPMGFPQQAAT
                     ASAEALARAHKTGHVPLTAMALHNQAYISASFAVDRQLLAIDPAEAENYCVEHGVTAY
                     EAWARFWRGLVVAEQGDLELAVTIMHEAMEAARRIGAGLFDSVQLGHIGAVLARSGQR
                     ETGLDLLAEAIRRSQQTNAR"
BASE COUNT          553 a          958 c          922 g          544 t
ORIGIN      
        1 ccgagcattt gtctgctggg acctccgaat cgcctcggca agcaaatcga gcccggtttc
       61 gcgctgaccc gagcgcgcaa ggaccgcgcc gatgtggccc agctgcaccg aatcaaatag
      121 gcccgctccg atccgacgcg ccgcctccat cgcctcgtgc atgatcgtca ccgcgagttc
      181 caagtcgccc tgctccgcga cgacaagtcc tcgccaaaat cgcgcccacg cctcgtacgc
      241 ggtaacgccg tgctcgacgc agtaattctc cgcctcggcg gggtcgatag cgagcaactg
      301 tcgatcgaca gcaaaagatg ctgagatgta tgcttggttg tgcagggcca tggcagtcag
      361 cggtacgtgc ccggtcttgt gcgctcgggc gagggcctcc gccgacgctg tcgccgcctg
      421 ctgcggaaaa cccatcggcc agagggtgac gccgagaaac gagagcgcgg cgatgctgtg
      481 attatggagc gatcgcttgt cgtcgacctc gcaaagcgac agcgatcgct cgaggtggtc
      541 acgtgccggt acaaacgcgc ccctcgccca taaggtattg cccatcaagg cattggccag
      601 gatcgagaac tccacttcct ggtgttgatg ggacagttca atcatctgct cggccagctc
      661 gccggtcgcg gcatcgtccc agcgcgtaaa acgggtaatc cacaggccgt aaagaacgcc
      721 catctgctcg cgggcgccgg cgttttcatg aagcagggcg cgcgcccgag agtaaacctc
      781 cagcacttcg tccgtggcat gcccttggat ggcccggatc gccgggccaa gggcgaggta
      841 gagccgcaat tcgcggcgat cgcgctcggg cgcggtcggc agcaggtgga tcagctcgat
      901 ccccttcttc aggtgcgcca tcgcctcgac gttcgccgac cgtcggagcg cccgttcgcc
      961 cgccttgcgc cagtattcga tcgcttcgtc gaaaagtccg gcctgcgagt agtgatgcgc
     1021 gagtatttcc ggttccaaat ccgaggtttc cgggaacctg ctttccagcg tcttggcgat
     1081 cagagcgtgc aactcttgcc gatgggcgcg cagcagggtg ctgtaggcag catcctggat
     1141 cagggcgtgc ttgaacatga tcgatgcccg gggcggggtg ccgcgccaga ataccaggcc
     1201 ggcgctcgcg agctggtcaa tcgcctcttg caggtgctcg gttctatcgg gcgtcagcgc
     1261 ctccaggatt tcatacgaga attctcgacc gagcgcggcg ccgatctggg caacatccct
     1321 tcccgccgga gacaagcggt ccaggcgggc catcagcgaa gcctgcaggc tggtcggtat
     1381 cgctagctgc gggaccgatc cattgcgccc gcgagcatcg ggcaatcgac cgaggagact
     1441 gttttcgagc aggctcttgg taagttcctc gatgaacaga ggaacgccat cggtgcgatc
     1501 aacgatctga tcgattatct cgggacgaaa cgtgttgctg ccgccggcga cccgcgcagc
     1561 gagcatcgcc gcatcgtgcc ggctcagtcg gctcaacgcc aaggtggtta cattcgacca
     1621 gtcaacccac ggtggctgga actcgggacg gaacgtcagc acgatcagca ccgggatatg
     1681 cggcacgcgc ttggcgagca tcaccaataa ttcgagcgaa ctggaatcga tccaatgcat
     1741 gtcttcgcaa atcagcagca cgggttgttc gtgcgccaga ccttccagat gccggaccgc
     1801 cgcggccaga aggcgttccc gtcgccgttg cggatccatg cttgccgcag gattgccgtc
     1861 tcctgtcggc aatcccaaca ggtcgccaaa caggatcacg gcttcgtcgc ctgctgcgga
     1921 attcgtcagc aggcgggcaa gcttctcgaa cttgacctcg ggggtatcct cgcgcgcgaa
     1981 acgggcggcg tgctcgaggt gtgcaatgaa cggatacagc gtgctgccga cgtgatgcgg
     2041 cgagcagaag tatcgcagcc gaatatagcc ttcgttaccg agctgttcct gaattgccgc
     2101 gatgattcgc gatttgccga tacccggttc cccggacagc tgcacgatct ggccctcgcc
     2161 ggccgtcacc cgctcccaaa gctgcctcag gagggcgagc tcttcatgac gaccgaccag
     2221 tggggtcatc gaggctgcgc ggagcgcctc aaaacggcta gccagcccgc tcgatcgcag
     2281 cacttgccag accggaaccc tgcgggcaaa ccccctgata tcgaggccgc cgacatcgcg
     2341 atattcgaag aagtctccga tcagttgccg ggtgatcgag tttatcagca acgaatttgg
     2401 cggagccaag ccctgtagcc ggaaggccaa gtttggtgtc tcgccgacaa tgccgcgttc
     2461 ctgtgcgtca ccggtgccga tcaggtcgcc gatgacgacc ggccccgtgg caatgccgat
     2521 ccgtatttcg agaggtccgc acgggaattc catcttgccg atgcggtcga tcagcgcgag
     2581 gccggcatga accgcgcgct cggcgtcgtc ctcataggcc tgcggatgac cgaagcagat
     2641 cagtaccccg tcgcccatgt agcggttgac ggtgccgtcg aattcacggg tcacctccga
     2701 cactaggccg ttgtacatgc cgagaacctg ccgcaagtct tccgggtcga ggcgcgatga
     2761 gagcggtgtc gatccgacca gatcgcaaaa catgatcgtg acctggcggc gctccgcccg
     2821 ctccggacgc gccgcaacgg cggctctctt gctgggcgcg gcggcttgtg ccgctccccc
     2881 ggtctctttc agaacaccaa ccgcccggag gatccgcttg cgatgtccaa gcaggacgcc
     2941 gagcttctcc agatcggatt cggtcagttc gagcagc
//
LOCUS       DASSGX010000126         6596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_349689, whole genome shotgun sequence.
ACCESSION   DASSGX010000126 DASSGX010000000
VERSION     DASSGX010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6596
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349689"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..1004)
                     /locus_tag="VFI12_02235"
     CDS             complement(192..1004)
                     /locus_tag="VFI12_02235"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HET7055248.1"
                     /translation="MNDRRWDELIAAARAASEATGLPVAEVSLDDIARHAGISRATLY
                     RRIGSRAALEDAIRAAGIDPGGRADVRERATEAAANLIEEGGLAALTLEAVAERAACS
                     VRALHTQIGGRDGLLGAIFERYSPLPQIEQAMSGGSMPLADGVRAIYDSILDSARVRP
                     GLLAAVVADVAFRPDGPTAEFVRATYLPRVANVLAPWLEARVQSGEIRPLPIPLLIKL
                     LVGPMGAHVFLGPAIARMTGLSQPSPDAVCEELTAAFLRAVARAPTELGLHD"
     gene            1193..1663
                     /locus_tag="VFI12_02240"
     CDS             1193..1663
                     /locus_tag="VFI12_02240"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055249.1"
                     /translation="MTLPRFLTVFGLLALLLSAIFISPSHQAAAQDASDLVVTLTPNV
                     TRAKIGDIVEFTVRVENVGTVTITNLEVGLGLPDALDARSVYCPFNPGVGNLVTDCLI
                     GNLEPGAATDVPFYVHVGSKEANGTVSATASADGIPAVIVTVAPIKVIGPTKVR"
     gene            complement(1703..3937)
                     /locus_tag="VFI12_02245"
     CDS             complement(1703..3937)
                     /locus_tag="VFI12_02245"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HET7055250.1"
                     /translation="MVSVQERHTRSQHDHDDPHVIQPFPERLEKSLGNPRLAANVTRY
                     QRNWRVNRDTSFQEVEFEQLRTEFKATKTRVQDRLDDYLAQFQKAAEAAGATVHHAGN
                     ARDANRIALEICRAHEAKQIVKSKSMVSEEIELNHFLEGQGIDCVETDLGEWIVQKAG
                     QRPSHIVGPALHMGRQDVAELLNDKMHVPVSQEDIPEQVHAIRDLIRPLFFTAGVGMT
                     GANALIAESGTIMMCTNEGNGRMSSSVPPVHIVLAGIEKIIPTFDDAIAQLRLLGRSG
                     TGQRMTVYTTFITGPTPGHEMHIILVDNGRRKMRAMPEFREALHCIRCGACANVCPPY
                     REVGGHVFGYIYTGAIGLVVTQFHHGLDAIAKPQSMCLSCNACETVCPVHIPLPQQII
                     DVRKMVVAKKGLSGPKRLVVAVIQRPKAFSLATRIGKRAQLPATRGGKYIRMRNLPKL
                     NRQTKWRSMPAVASKSVMDRVDPGNFFPQMASVVPNQVVGKTVAVFPGCMTNSLYPEQ
                     GMAVTDVLRGLGMRVMVPKGLNCCGLPASNMGDDPAARRMAKQTIQALERSQCEYIVS
                     GSASCVAMIMQDYIHILRHEPGWQRRATTLAGKVRDLTTFLVHDAQLQPGALAEDGSA
                     ETVTYHDSCQGLNALGLSAEPRYLLNDVLGIEIRELDENRLCCGFGGSFGFDFPEVSE
                     RLMNAKLDNAAATGARTVVTDNQGCIMHLRGGCDATHRPMSVNHIAELIAQRIRSRQS
                     NSHA"
     gene            complement(4000..4257)
                     /locus_tag="VFI12_02250"
     CDS             complement(4000..4257)
                     /locus_tag="VFI12_02250"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HET7055251.1"
                     /translation="MAEVATSKDASTRYAVVLTPDPESGGYTVTVPSLPGVFTHGDTV
                     EEALSMARDAIACFLELPEGAGAVSEDRVEAIMATVAVANS"
     gene            complement(4474..5040)
                     /locus_tag="VFI12_02255"
     CDS             complement(4474..5040)
                     /locus_tag="VFI12_02255"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HET7055252.1"
                     /translation="MDLEHSHLLIAQFTERATPLGTVVERAANSAGAARIIAALVQAA
                     GSDRISVAPAVGGRAPDLVAALSTAGIDPQAIQDTSDARDQPVGLSIATRAVAETGSV
                     LLDEGELADRSASLMTLHSIVLIAAPDIVPSLDSTPSLLREIACRPGGGYASFVTGPS
                     RTADIEMSLTVGVQGPGQVTILFVDNLR"
     gene            complement(5040..>6596)
                     /locus_tag="VFI12_02260"
     CDS             complement(5040..>6596)
                     /locus_tag="VFI12_02260"
                     /inference="COORDINATES: protein motif:HMM:NF045169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HET7055253.1"
                     /translation="AVRGSVPFNDVAIILDPADVVGIAKRPLLPGTVLLRENGDELRI
                     SQMIPPGHKFALTEIENDAPVRRYGQIIGFATTDIKPGQHVHVQNMSIKEQGSLKVDY
                     AIGTEYKPETFVPASARRTFMGFRRKDGRIGTRNFVAVLASVNCSSSATAAVVDYFRQ
                     PGALKNYPNVDGVIGYPHKGGCGAHIGSRDLQLFQRTLAGMVDHPNVAGYVVLSLGCE
                     VNQPSDMIETTTMKEDQPLVITIQQDGGYAKTVELGIEAVKRLLPEANKSVREEVTAD
                     HLMVALQCGGSDGWSGVTANPALGKAADLVVQQGGTVVLGETTEVYGAEHLLTRRAKS
                     PAVAEKLINMIHWWEDYTAYFGGTIDNNPSPGNKLGGLTTIYEKSLGAVAKAGSMPLN
                     QVVGYGERVTEKGFVHMDTPGYDPVSVTGQVAGGCNVVCFTTGRGSAFGFKPSPSIKL
                     ATNSELYGKQEPDMDINCGRVLDGITLDQMGQEIFEEILAVASGKRSKSELAGVGEEE
                     FNPWILGAML"
BASE COUNT         1078 a         2047 c         2273 g         1198 t
ORIGIN      
        1 tcgagaccga tgatcagcag gctgagcgaa aggatcgcca gcgtccacca gcgccggcgg
       61 tatttcagat catcagtgcg cgattgcgcc ggggccgtgg tggttggggt tcgacgggtg
      121 gttgcaaaca tggttccact ctcctcatgc gggcgtggca ttccaactgg tgatacgaac
      181 agacgtgggg gttagtcgtg caggccgagc tcggtgggcg cccgcgcgac cgcccgcagg
      241 aacgcggcgg tcaactcctc acagaccgcg tccggagacg gctgggacag gccggtcatg
      301 cgcgcgatcg ccggtccaag aaagacatgc gctcccatcg ggccaaccag gagcttgatc
      361 agcagcggga tcggcaatgg ccggatttcg ccggactgca ccctggcttc gagccacggc
      421 gccagcacgt tcgccactcg tggcaaatag gtggcgcgaa cgaactcggc ggtcgggcca
      481 tcggggcgaa aggccacgtc ggccacgacc gccgcgagca acccgggccg gacacgcgcg
      541 gagtcgagga tcgagtcgta gatggcccgg acgccatccg cgagcggcat cgaaccgccg
      601 gacatcgcct gttcgatctg gggcagcggg ctgtaccgtt cgaagatcgc gccgagcagg
      661 ccgtcccgcc cgccgatctg ggtgtgcagc gcgcggaccg agcacgcggc gcgctcggcc
      721 acggcctcga gggtcagcgc ggcgagaccg ccttcctcga tcaggttagc cgcggcctcg
      781 gtcgctcgtt cgcggacgtc ggcgcggccg ccggggtcga tgccggcggc gcggatcgcg
      841 tcctcgaggg ccgcgcggga accgatccgg cgatagagcg tagcccggga gatgccggca
      901 tgtcgcgcga tatcgtcgag cgaaacctca gcaaccggga ggccggttgc ctcgctcgcc
      961 gctcgtgctg ccgcaatcag ctcatcccac cgccggtcat tcatgagacg atactacgtg
     1021 tcatacgtag tgctgtcaag tatcacgtct ttaccccgag ctgttctctt cagaggcaca
     1081 cacgtgtgtt gctatggtga ctgcttgaca cgtcaaacat ccgtcgatag agtgggcgca
     1141 cctacctgca ggcgaatgtg ttcatctcgc agacaccgga aggagttctc gcatgactct
     1201 cccgcgtttc ctcaccgtct ttggtctgct tgcccttctc ctcagcgcca tattcatctc
     1261 gcccagccac caagcagcgg cgcaggacgc gagcgatctg gtcgtcaccc tgacgcccaa
     1321 cgtgacccgg gccaagatcg gcgacattgt cgagttcacc gtcagggtgg agaacgtcgg
     1381 cacggtaacg attacgaatc tcgaagtggg gctaggcctt ccagacgcgc tcgacgcgcg
     1441 gtcggtgtac tgcccgttca accccggcgt cggcaatctg gtgacggact gcctcatcgg
     1501 caacctggag ccgggcgctg ccacggacgt gccgttctat gtccatgttg gatcgaagga
     1561 agccaatggg accgtttccg cgaccgcgag tgccgacggg attcccgccg tgatcgtcac
     1621 ggttgcgcca atcaaggtta tcggacccac aaaggttcgc tgaggctcaa gcgacgtttc
     1681 cgctcccgtt ccgcgaaccc cactaggcgt ggctgttgga ttggcgggag cggatgcgct
     1741 gggcgatcag ctcggcgatg tggttgacgg acattgggcg gtgcgtggcg tcgcagccgc
     1801 cgcgcaggtg catgatgcag ccctgattgt cggtcacgac ggtgcgggcg ccggttgcgg
     1861 cggcgttgtc cagcttggcg ttcatcagcc gctcggagac ctcggggaag tcgaacccga
     1921 aggaaccgcc gaagccgcag cagaggcggt tctcgtccag ctcccggatc tcgatgccaa
     1981 gcacgtcgtt gagcagatag cgcggctccg cggagaggcc gagcgcgttc agaccctggc
     2041 acgagtcgtg gtacgtcacc gtctcggctg agccgtcctc ggccagtgcg ccgggctgga
     2101 gctgggcgtc gtggaccagg aacgtcgtca ggtcgcggac cttgccggcc aacgtggttg
     2161 cgcggcgctg ccagcccggc tcgtgacgca ggatgtggat gtagtcctgc atgatcatcg
     2221 ccacgcagct cgcgctgccg gagacgatgt actcgcactg cgaccgctcg agcgcctgaa
     2281 tcgtctgctt ggccatccgt ctggcggcgg ggtcgtcgcc catgttgctg gccggcaggc
     2341 cgcagcagtt gagccccttc ggcaccatta cacgcatgcc gagaccgcgc aggacgtcag
     2401 tgacggccat cccctgctcc gggtacaggc tgttggtcat gcagcccggg aagacggcga
     2461 ccgtcttgcc gacgacctga ttggggacga ccgatgccat ctgcgggaag aagttgccgg
     2521 gatcgaccct gtccatgacc gacttcgatg cgaccgccgg catggagcgc cactttgtct
     2581 ggcggttgag cttgggcagg ttgcgcatcc ggatgtactt gccgccgcgc gtggccggaa
     2641 gctgggcgcg tttgccgatc cgggtggcga ggctgaacgc cttcgggcgc tggatgacgg
     2701 cgacgaccaa ccgctttggc ccggatagtc ccttcttcgc gacgaccatc ttgcgtacat
     2761 cgatgatctg ctgcgggagc gggatgtgga cggggcagac cgtctcgcag gcattgcagg
     2821 agaggcacat gctttgcggc ttggcgatcg cgtccagccc gtggtggaac tgcgtgacga
     2881 ccaggccgat cgcgccggtg tagatatagc cgaagacgtg cccgccgact tcccggtacg
     2941 gcgggcaaac gttcgcgcag gcgccgcagc ggatgcagtg gagggcttcg cggaattccg
     3001 gcatcgcgcg catcttccgg cggccgttgt cgaccaggat gatgtgcatc tcgtgaccgg
     3061 gagtgggtcc ggtgatgaag gtcgtgtaga cggtcatgcg ctgaccggtg ccgctgcggc
     3121 cgagcaagcg aagctgggcg atcgcgtcat cgaaggttgg gatgatcttc tcgatgccgg
     3181 cgagaacgat gtggaccggc ggcacggacg acgacatgcg cccgttgccc tcgttggtgc
     3241 acatcatgat ggtgccggat tcggcgatca gggcattcgc gccggtcatg ccgacgccgg
     3301 cggtgaagaa gagcgggcgg atcaggtcgc ggatggcatg gacctgctcg gggatgtctt
     3361 cctgcgagac cgggacgtgc atcttgtcat tgagcagctc ggcgacgtcc tgccggccca
     3421 tgtgcagggc cggaccgacg atgtggctcg gacgctggcc agccttctgg acgatccact
     3481 cgccgaggtc ggtctcgacg caatcgatgc cctggccctc gaggaaatgg ttgagctcaa
     3541 tctcctcgct gaccatggac ttggatttga cgatttgctt cgcctcgtgg gcgcggcaga
     3601 tctcaagcgc gatccggttg gcgtcgcgcg cgttgccggc atgatggacc gtcgcacccg
     3661 ccgcctcggc cgccttctgg aactgggcga gatagtcgtc gagccggtcc tggacgcggg
     3721 tcttggtcgc cttgaactcg gtccggagct gctcgaactc gacttcctgg aacgaggtgt
     3781 cgcggttgac gcgccagttg cgctggtagc gggtgacatt ggcggcgagg cgcgggttgc
     3841 cgagcgattt ttcgaggcgc tcggggaacg gctggatcac atgcggatca tcgtgatcgt
     3901 gctgggagcg ggtgtgtcgt tcctgaaccg agaccatggc gggctgcttt cgctacatca
     3961 gtgcatcaat aagctggcaa cggaacgtgg acggtagctt catgagttgg caacggcgac
     4021 cgtcgccatg attgcttcaa cgcggtcctc agagactgcc ccggctcctt caggaagctc
     4081 aaggaagcaa gctatggcgt cccgcgccat cgacaacgcc tcctcaaccg tgtcaccgtg
     4141 ggtaaacacg cctggcaatg acggaaccgt cacagtgtat ccgccgctct cagggtcggg
     4201 cgtgagcacc accgcgtatc gggtggacgc atctttggat gtagctactt cagccatccg
     4261 atgaactcct cgacgctcat accagcgtga tccaggatcg cacgcaatgt tcccgccggc
     4321 atgtcgcgtc ccctatgctg gggcacaatc acggttactt gcccacgcac catcttgaca
     4381 tgactgcccg actgatgcgt tgcctgaaat ccggctcgct cgagcgctcg ccgcacctcc
     4441 gtttgcttaa cgattggtac tggcggcatt gcgctacctg aggttgtcta cgaacaggat
     4501 agtgacctgg ccggggcctt gcacaccgac cgtgagcgac atctcgatgt cggcggtgcg
     4561 gctggggccg gtcacgaagc tggcgtagcc tccccccggc ctgcaggcga tctcgcgaag
     4621 cagggacggc gtgctgtcga ggctggggac aatgtccggc gcggcgatga ggacgatgct
     4681 gtggagggtc atcaggctgg cggagcgatc agccagctct ccctcgtcaa gcaggacgga
     4741 gccggtctcg gcgacggcgc gggtcgcgat cgagagaccg accggctgat cgcgggcatc
     4801 gctggtgtcc tgaattgcct gcggatcgat gccggcggtg gaaagcgccg cgacgaggtc
     4861 gggagcacgt ccaccaaccg ccggagcgac cgagatgcgg tccgatccgg cggcctggac
     4921 gagcgcggcg atgatcctgg ccgcgccggc gctgtttgca gcgcgttcga ccacggtgcc
     4981 cagcggcgtg gcgcgctccg tgaattgcgc gatcaaaagg tgcgagtgct caagatccac
     5041 tagagcatcg cgcccaggat ccaggggttg aactcctctt cgcccacgcc ggccagctcg
     5101 ctcttggagc gcttgccgga ggcgacggcc aggatctcct cgaagatctc ctgccccatc
     5161 tggtcgagcg taatgccgtc gagcacccgg ccgcagttga tgtccatgtc gggctcctgc
     5221 ttgccgtaga gctcgctatt ggtggccagc ttgattgagg gggacggctt gaagccgaag
     5281 gccgagccgc gaccggtcgt gaagcagacg acattgcaac cgccggccac ctggccggtg
     5341 acggagaccg ggtcgtagcc gggcgtgtcc atgtggacga agcctttctc ggtcacgcgc
     5401 tcgccgtagc cgacgacctg gttgagcggc atcgagccgg ccttggcgac cgcgccgagc
     5461 gacttctcgt agatcgtggt cagaccaccg agcttattgc cgggggatgg gttgttgtcg
     5521 atcgtgccgc cgaagtatgc ggtgtagtcc tcccaccagt ggatcatgtt gatcagcttc
     5581 tcggcgaccg cggggctctt ggcgcggcgg gtgagcaagt gctcagcgcc gtagacctcg
     5641 gttgtctcgc cgaggacgac tgtgccgccc tgctggacga ccagatcggc cgccttgccg
     5701 agggccgggt tggcggtcac gccggaccaa ccgtccgagc cgccgcactg cagcgcgacc
     5761 atcaggtgat cggccgtgac ttcctcacgg accgacttgt tggcctccgg gagcagtcgc
     5821 ttgaccgcct cgatgccgag ctcgaccgtc ttcgcgtagc cgccgtcctg ctggatggtg
     5881 atcacgagcg gctggtcctc cttcatggtc gtggtttcga tcatgtcgga gggctgattg
     5941 acctcgcagc cgaggctgag gacgacgtag ccggccacgt tcgggtggtc gaccatgccg
     6001 gcgagcgtgc gctggaagag ctggagatcg cgcgagccga tatgcgcgcc acagccaccc
     6061 ttgtgcgggt agccgatcac gccgtcgacg ttcgggtagt tcttgagcgc gcccggctgc
     6121 cggaagtagt cgaccacggc ggccgtggcg gacgacgagc aattgaccga ggcgaggacg
     6181 gcgacgaagt tgcgggtgcc gatccggcca tccttgcgcc tgaagcccat gaaggtgcgg
     6241 cgcgcggagg cgggcacgaa cgtctcgggc ttgtactcgg tgccgatcgc gtagtcgacc
     6301 ttgagcgatc cctgctcctt gatcgacatg ttctggacgt ggacatgctg gccgggcttg
     6361 atgtcggtcg ttgcgaaacc gatgatctga ccatagcgac gaaccggggc atcgttttcg
     6421 atctcggtca gcgcgaactt gtgaccgggc gggatcatct gcgagatgcg cagctcatcg
     6481 ccgttctcgc ggagcaggac cgtgcccggc aggagcggcc gtttggcgat gccgacgacg
     6541 tccgccgggt cgaggatgat ggcgacgtcg ttgaagggga cgctgccgcg gacggc
//
LOCUS       DASSGX010000127         4648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1219461, whole genome shotgun sequence.
ACCESSION   DASSGX010000127 DASSGX010000000
VERSION     DASSGX010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4648
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219461"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1808
                     /locus_tag="VFI12_02265"
     CDS             <1..1808
                     /locus_tag="VFI12_02265"
                     /inference="COORDINATES: protein motif:HMM:NF013400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055254.1"
                     /translation="GMIPFDPTPFGTVGLFDIDWLNTPEFGALLDMFAASPGAITGMR
                     VFGALTAGELDSYTPATSGMVWTDPVGPIDFSATFSALDQLTSRGLTPFVSLGFFPPA
                     VSPVPVVPPASWDRWQRLVQAFFTQLAADPRFGAAAIADWWFEVWNEPNEGRFWQGTP
                     EQYFDLYRATSEAVTGPELSIRLGGPAIAYQPQRNPDDGAPWIERFLRFIATDESLRL
                     DFISFHRKGTVGTDPPDPDRLRKAAEETARLALAIIPERCDGLAIVNDEADEKVGFEQ
                     PYAPRMDRAASAWLCSAMAQQVALTARYRDAGLRFISAADNANLQLVQAPFDGRRSVV
                     TVGPEDPTDLIKLPVFAGYEVVRLLGEEASRRAGEQRRMGNATTSPVRAAAREGGDGV
                     YQLATISGAQATVIVANCPDPGSDGPPPAIEWRVMGLPWDEVNIALFVIDGTHSNAYT
                     AAGGSVADPYPVPSPADLPAIRMAAELAAARPIMRGVPIPDGVYTERLSITQNATLCL
                     WITPVDATVPAAPAWIRVDPRETRTILRWEPCRAPWFYSYEVYRTDGGRPVERLGPDP
                     LRAAMWVVDHPGPSATSYGVRVVSASGITGPFAII"
     gene            1964..3535
                     /locus_tag="VFI12_02270"
     CDS             1964..3535
                     /locus_tag="VFI12_02270"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7055255.1"
                     /translation="MEATAWGSLHRLISAQGERRPISRVTLRRVAAFAKPMRGRLLQF
                     LLLSIVSAVLAVATPVLAGRVVDAIVNGESERRVITLAALIAGIAIADAGLGIVTRWL
                     SANIGEQLILDLRTAVFDHVQRMPIAFFTRTRTGALVSRLNNDVIGAQRAFSDTFSGI
                     VSNVVMLILTLVVMLRISWQITLLALILLPIFVLPARRMGARLAALRTEAANHNAEMS
                     TQMTERFSAPGATLVKLYGRPAEESAEFAKRAKRVRDIGVRSAMLQWVFVTALTLVSA
                     LALAVVYGLGGYSALKGNLEAGSVVALALLLTRLYAPLTSLANARVEVMGALVSFDRV
                     FEVLDLQPLITEKPDARSVPPGPASVEFDHVSFSYPSAEKVSLASLESVAVLDTRGGV
                     EVLHDISFRVDPGEMVALVGSSGAGKSTIAQLVPRLYDVDRGAVRISGVDVRDLTLES
                     IRQAIGMVTQDGHLFHDSIRANLKLAAPEATDDELWHALRRARLDGVIAALPDGLETV
                     IGERGYRLSGGSGSG"
     gene            3553..3870
                     /locus_tag="VFI12_02275"
     CDS             3553..3870
                     /locus_tag="VFI12_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055256.1"
                     /translation="MAKPRVVILDEATAHLDSRSEAMVSEALGEALTGRTVIVIAHRL
                     STIRAADQILVIEGGRIVERGRHEELLMKGGRYAELYRTQFQTSDAGEKASDVGLQTS
                     DVG"
     gene            complement(3948..4502)
                     /locus_tag="VFI12_02280"
     CDS             complement(3948..4502)
                     /locus_tag="VFI12_02280"
                     /inference="COORDINATES: protein motif:HMM:NF021386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HET7055257.1"
                     /translation="MDDGTPLKVLAALANVERIRIVALIAERSLSAAEIVAETGLGQE
                     AVTAHLTVLGQAGLIATSKDRHERRLSLRTERLKEVAAICQGQSETGDSSDGAVLPPG
                     IRQFFRDGRLTNIPSKQSRYIEILSVLVEDFEPETAYPETEVNQTLLRRNEDFATLRR
                     DLVDFGFMTRANGIYRRTGSAPPK"
BASE COUNT          718 a         1498 c         1609 g          823 t
ORIGIN      
        1 cggggatgat tccgtttgat ccgaccccgt tcggcacggt cgggctgttc gacattgact
       61 ggttgaacac acccgagttt ggtgcgttgc tcgacatgtt cgccgcctcg ccgggggcaa
      121 tcaccgggat gcgtgtcttc ggcgcgctca cggccgggga actcgattcc tacacgccgg
      181 cgacgagcgg catggtctgg accgatccgg ttggaccgat cgacttttcg gccaccttct
      241 ccgcactcga ccaactgacg tcgcgcgggt tgacaccctt cgtgtcgctc ggcttcttcc
      301 cgccggccgt ctcgcccgtg ccggttgtgc cgccagcttc gtgggaccgg tggcagcgac
      361 ttgtccaggc cttcttcacg cagctcgcgg ctgatccccg attcggagcg gcggcgatcg
      421 cggactggtg gttcgaggtc tggaacgagc cgaatgaagg ccgattctgg caggggacgc
      481 cagagcagta cttcgatctc taccgcgcga cctccgaggc cgtaaccggg ccggagttgt
      541 cgatccggct cggcggtccg gccatcgcct atcagccgca gcggaatccg gacgacggcg
      601 cgccgtggat cgagcggttc ctgcgtttca tcgcgacgga cgaatcgctc cggctggact
      661 tcatctcgtt ccaccgaaag gggacggtcg ggaccgatcc gcccgatcca gaccgcctcc
      721 ggaaggccgc cgaggagaca gcccggctgg cgctcgcgat tatcccggag cgatgcgacg
      781 ggctggcgat cgtcaatgat gaggccgacg aaaaggtcgg gttcgagcag ccctacgccc
      841 cgcgcatgga ccgggccgca tccgcctggc tctgctcggc catggcccag caggtcgctc
      901 tgaccgcgcg ataccgggat gcgggcctgc gcttcatctc cgccgccgac aacgccaacc
      961 tccagctcgt gcaggctccg ttcgacggtc gccgctcggt cgtgacggtc ggtccggagg
     1021 accccacgga cctgatcaaa ctgccggtgt ttgcagggta tgaggtggtg cggctcctgg
     1081 gcgaggaggc gagcaggcga gcaggcgagc agcgaaggat ggggaatgcc acaacgtcgc
     1141 cggtgcgtgc ggcggcacgc gagggtgggg atggcgtgta ccaactggcg acgatttccg
     1201 gggcgcaggc gaccgtcatc gtcgcgaatt gtcccgatcc ggggtcggat ggcccgccac
     1261 cggcgatcga gtggcgggtg atggggttac cgtgggacga ggtcaatatc gcgttgttcg
     1321 tgatcgatgg aacccactcg aacgcgtaca cggcggccgg gggatcggtg gccgatccat
     1381 acccggtccc gtccccggcc gatctgccgg cgatccggat ggcggcggag ctggctgccg
     1441 cacgacccat catgcgcggg gtgccgatcc cggatggcgt gtacacggag cgactctcga
     1501 tcacgcagaa tgcgacgctt tgcctctgga tcacgccggt tgatgccacc gtgcccgccg
     1561 cgccggcatg gatacgcgtc gatccgcggg agacgcggac gatcctgcgc tgggagccct
     1621 gtcgcgcacc atggttctac agctacgagg tctatcggac ggatggtggg cggccggtgg
     1681 agcgcctcgg ccccgacccg ctccgcgccg caatgtgggt cgtcgatcac cctgggccat
     1741 cggcaacgtc gtatggtgtc cgggtggtga gcgcgtcggg cattaccggt ccgttcgcaa
     1801 tcatctgatg cctgtacgat cgcaatcccg atggccccac ctacccgatt gcggaatgcc
     1861 gcccatgggg agcaggagat aggatcagga gagcgcccgg aatcaggcgt tgcctggaga
     1921 gaagaaatag atggcagggt catgtggccc tgtggagaca tagatggaag cgacagcctg
     1981 gggctctcta catcgattga ttagcgcgca gggagagcgg cggccgattt cgcgcgtgac
     2041 cctgcggcgg gtggcggcct tcgccaaacc gatgcgcggg cggctcctcc agttcctgct
     2101 gctcagcatc gtctcggccg tgctggccgt cgcgacgccg gtgctggccg gccgggtggt
     2161 cgatgcgatc gtcaacggcg aatcggagcg ccgcgtgatc accctcgccg cgctgatcgc
     2221 cgggatcgcg atcgccgacg ccgggctcgg catcgtcacc cgttggctct cggccaacat
     2281 cggcgagcaa ttgatcctcg acctgcgtac ggccgttttc gatcatgtcc agcgcatgcc
     2341 gatcgccttc ttcacccgca cccggaccgg cgcgctggta agccggctca acaacgatgt
     2401 gatcggcgcg cagcgggcct tcagcgacac cttctcgggc atcgtcagca atgtcgtgat
     2461 gctgatcctg acgctggtcg tcatgctccg catctcctgg cagatcaccc tgctggcgct
     2521 aattctgctg ccgatcttcg tgctgccggc gcggcggatg ggtgctcgcc tggccgcgct
     2581 ccggaccgag gccgccaatc acaacgccga gatgagcacg cagatgaccg agcgcttctc
     2641 cgcgcccggg gcgacgctcg tcaagctcta cggccggccc gcggaggaat cggccgaatt
     2701 cgccaagcgg gcgaagcggg tgcgggacat cggcgtgcgc agtgccatgc tccagtgggt
     2761 cttcgtgacc gcgctgaccc tcgtttcggc gctcgcgttg gccgtggtct acgggttggg
     2821 cggctacagc gcgctcaagg gcaacctcga agccggctcc gtcgtggcgc tggcattgct
     2881 cctcactcgc ctctatgcgc ccttgacctc gcttgccaat gcccgagtcg aggtgatggg
     2941 cgcgctggtc agcttcgacc gcgtctttga ggtgctcgat ctccagccgc tgatcaccga
     3001 gaagcccgat gcgcgatcgg tcccaccggg tccggcgtcg gtcgagttcg accatgtcag
     3061 cttcagctat ccctcagccg agaaggtctc gctggcgtcg ctggagtcgg tcgcggtgct
     3121 cgatacgcgg ggcggggtcg aggtgctgca cgacatctcc ttccgggtcg atccgggcga
     3181 gatggtcgcg ttggtcggct cgtccggcgc cggtaaatcg acgatcgcgc agctcgtgcc
     3241 gcggctgtac gacgtggatc gcggagcggt ccgcatctcg ggcgtggacg tgcgcgacct
     3301 gacgctggaa tcgatccggc aggcgatcgg gatggtcacg caggacggcc atctcttcca
     3361 cgactcgatt cgggcaaacc tgaagctggc cgcgcccgag gcgaccgacg atgagctctg
     3421 gcacgccctg cggcgagccc ggctggacgg cgtgatcgcg gcgctgccgg atggcctcga
     3481 aacggtcatc ggcgagcgcg gctaccggct ctccgggggg agcggcagcg gctgacgatc
     3541 gcgcggctgc tcttggccaa gccccgggtc gtgattctcg atgaggcaac ggcgcacctc
     3601 gattctcgtt ccgaggcgat ggtctccgag gcactgggcg aggcgctgac gggccggacc
     3661 gtgatcgtga tcgcgcaccg gctgagcacg atccgcgccg ccgatcagat tctggtcatc
     3721 gaagggggcc ggatcgtcga gcgaggccgc cacgaggagt tgctgatgaa gggtggccga
     3781 tatgcggaac tctaccgcac gcagttccag acgtcagacg ccggggagaa ggcgtcagac
     3841 gtcggacttc agacttcaga cgtcggttga gacctcttga aacgtagccc tgggtggccc
     3901 cgcccagcat tggagaacaa catccgggcg ggccgtccgg agctacatca tttgggcggg
     3961 gcggagccgg tgcggcggta gattccgttg gcgcgggtca tgaagccgaa gtcgacgagg
     4021 tcgcggcgga gggtggcaaa atcctcgttt cggcggagca gggtctggtt gacctcggtt
     4081 tccgggtacg cagtctccgg ctcgaaatct tcgacgagta cgctcaggat ctcgatgtag
     4141 cgggactgct ttgatgggat gtttgtcagg cgaccgtccc ggaagaattg gcgaatccca
     4201 ggcggtagaa cggcgccgtc ggacgagtcg ccggtctcgg attgcccttg gcatatcgcc
     4261 gcgacttcct ttagccgctc cgttcgcagc gagaggcgcc gctcatgccg atccttgctg
     4321 gtcgcgatca atccggcttg cccgagcacg gtcagatgcg ccgtaaccgc ctcttggcca
     4381 aggcccgtct ccgccacgat ctcggcggca gacagcgacc gctccgcgat cagcgccacg
     4441 atccggatcc gctcgacatt cgccagcgcg gccaacacct tcaacggagt gccgtcgtcc
     4501 atgcgcaaac tttaccgcga gcgggtctcc ggacgggccg gccggataat acggccgcat
     4561 tgacggtctg ttccgaggac cgggtccgcg taccgggaac ctgcgtgggc gggaacgatt
     4621 atcgccctgc gggcgatcgc ggggcatg
//
LOCUS       DASSGX010000128         2035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_894786, whole genome shotgun sequence.
ACCESSION   DASSGX010000128 DASSGX010000000
VERSION     DASSGX010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894786"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="VFI12_02285"
     CDS             <1..730
                     /locus_tag="VFI12_02285"
                     /inference="COORDINATES: protein motif:HMM:NF015566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET7055258.1"
                     /translation="DILGPTVIMGFRPLIAGTGAGIIVGDVNLGMAIGATLELTALGV
                     YTYGGATIPDYQTGAIIGTALAAAGGGDLGAKTAIGLTIGLPAAVLLASLDPIGRFLP
                     TFWIHRADGYAAEGNSSGLSWMHWTAFIPWAAVRAIPTFLAALAGSTKTVADLQNSIP
                     QGFIDGMTLVGAILPAVGFAMLLKIMPVGRYWYMLLIGFVLYAYLSMPLLGIALFGVA
                     IAFLFVTLRPRPAAIPPAPATEAV"
     gene            730..1602
                     /locus_tag="VFI12_02290"
     CDS             730..1602
                     /locus_tag="VFI12_02290"
                     /inference="COORDINATES: protein motif:HMM:NF015570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HET7055259.1"
                     /translation="MTSTNPPIRLERRDLLASFWRYFWSFQISWNYERMQALGFAYAM
                     EPVLRKLFPDQADYEEALARHLVFFNTNPVVGAPLIIGSAIAMEEAGAPASAEGVKIA
                     LMGPLAGVGDTIIWALYNSIIFTIGASLALQQNILGPIFVIVMVAVPYTVVRYWQFMW
                     AYAQGRTLATSLASGMLERVTEGATILGLIVIGGFIPSIVHLVVKGSYSQTLTVTGPD
                     GTSSQVTNTVPIQTQLDAILPFMLPVLLTGIVYWLLRRYNLNPLWAIAIVFVFGVAAG
                     WLGWFAPSLPPPAA"
     gene            1681..>2035
                     /locus_tag="VFI12_02295"
     CDS             1681..>2035
                     /locus_tag="VFI12_02295"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET7055260.1"
                     /translation="MSDRYFQALDEILARVRVANQDSIAGAAAIVADTIAADGIVHAF
                     GSGHSQLLAIELAGRAGGLAGIQVILDPGQGKAETLEGYAATIVRDYTFEPRDCLIVI
                     SNSGRNPSPIEMAMIG"
BASE COUNT          305 a          707 c          649 g          374 t
ORIGIN      
        1 cgacatcctc ggcccgactg tgatcatggg cttccggccc ctgatcgccg gcacgggcgc
       61 ggggatcatc gtgggcgatg tgaacctggg catggcgatc ggcgccaccc tggagctgac
      121 cgctctgggc gtgtacacct acggtggcgc gacgatcccg gattaccaga ccggcgccat
      181 catcggcact gcgctggccg ccgcgggagg cggcgacttg ggggcaaaga cggcgatcgg
      241 cctgaccatt ggcctgcctg ccgcggttct gctcgcctct ctggacccca tcggtcggtt
      301 cctgccgacc ttctggatcc accgggcgga tggctacgcg gccgaaggta actcgagcgg
      361 cctctcctgg atgcactgga cggcattcat tccatgggcc gcggttcgag ccatcccgac
      421 cttcctggcg gcgctcgccg ggagcaccaa gacggtcgct gaccttcaga actccatccc
      481 gcagggcttc atcgatggca tgaccctggt gggagccatc ctcccggcag tcggcttcgc
      541 gatgctgctg aagatcatgc cggtggggcg gtactggtac atgctgctga tcggattcgt
      601 gctgtatgcc tacctgagca tgcccctgct gggcatcgcc ctcttcggcg tggcaatcgc
      661 cttcctgttc gtgacccttc ggccgcgacc cgcagccatt ccaccggcac cggcgacgga
      721 ggcagtctga tgacgtcgac gaatcctccc atccgactcg agcggcgaga tctcctcgcg
      781 tccttctggc gctacttctg gtcgttccag atctcctgga actacgagcg gatgcaggca
      841 ctcggcttcg cgtatgcgat ggagccggtc ctgcgcaagc tcttcccaga tcaggccgat
      901 tacgaggagg cgctggcacg ccacctggtc ttcttcaaca ccaaccccgt cgtcggggca
      961 ccgctgatca tcggatcggc gatcgccatg gaggaggcag gcgcgcctgc ttccgcggag
     1021 ggggtcaaga tcgcgctcat gggtccgctc gccggagtcg gcgacacgat catctgggcg
     1081 ctctataact cgatcatctt cacgatcggc gcctcgctgg cgctgcagca gaacatcctg
     1141 ggaccgatct tcgtgatcgt gatggttgcg gttccgtata cggtcgttcg ctactggcag
     1201 ttcatgtggg cctacgccca gggccgaacg ctggccacga gccttgcctc cgggatgctc
     1261 gagagagtga ccgagggcgc cacgatcctg gggctgatcg tcatcggcgg cttcatcccg
     1321 tcgatcgttc acctcgtcgt gaagggctcg tactcgcaga cgctgaccgt gaccggcccc
     1381 gacggcacgt cctcgcaggt gaccaacacg gtgccgatcc agacgcagct ggacgcgatc
     1441 cttccgttca tgctgccggt cctgctgacc ggcatcgtct actggctcct gcgccgctac
     1501 aacctgaacc cgctgtgggc gatcgccatc gtcttcgtgt tcggggtcgc cgccgggtgg
     1561 ctcggctggt tcgcaccgtc cctgccgcct ccggctgcct gaccgacgcc gtgtgcaatc
     1621 gacttcgggt ggcggatccg tccgccaccc gaggccgtac caggcccgaa cggaggaccg
     1681 atgtctgacc gatacttcca agccctggac gagatcctgg cgcgggtgcg cgtcgcgaac
     1741 caggactcga ttgccggcgc ggccgcgatc gtggccgaca ccatcgccgc tgacggcatc
     1801 gtgcacgcct tcggcagcgg gcactcacag ctgctggcca tcgagctcgc cgggcgcgcc
     1861 ggcggcctgg ccggcatcca ggtcatcctc gacccggggc agggcaaggc cgagaccctg
     1921 gagggctacg ccgccaccat cgtgcgcgac tacacgttcg agccgcgcga ctgcctgatc
     1981 gtcatcagca actccggccg caacccctcg ccgatcgaga tggcaatgat cgggc
//
LOCUS       DASSGX010000129         3231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_676435, whole genome shotgun sequence.
ACCESSION   DASSGX010000129 DASSGX010000000
VERSION     DASSGX010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676435"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..536)
                     /locus_tag="VFI12_02300"
     CDS             complement(57..536)
                     /locus_tag="VFI12_02300"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HET7055261.1"
                     /translation="MSEIACTAASPHAHRGVPGPAWIEATLGQLRREKYRITQPRVAV
                     LRWIGGREAPFTAEEAVAEIDQSLGAGSRATVYRFLFWLREQGWLARVHRTDRDHALV
                     RQLPGHHQVVCVECGDTLVIGGCDLTPLIGQSLSGTGFAVEGHQLEIFGRCRTCAHV"
     gene            complement(533..1237)
                     /locus_tag="VFI12_02305"
     CDS             complement(533..1237)
                     /locus_tag="VFI12_02305"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HET7055262.1"
                     /translation="MPELREQPRGQGDLGRRVLFARVGGEVAARSRRATDGYFCPAHG
                     RMPVLLAPAEGDTGMSASESKLSPDLHREIAAEQIGQVAIAIVTVSDSRTPETDTNMQ
                     YLKPEIESLGHLVSGYMLIKDEPDQVEAVLNRFADSEARMILFNGGTGIAPRDTTYDV
                     LARKIEKPMPGFGELFRMLSYEQVGAAAMLSRATAGVYRGKLVISTPGSNAAVRLAWE
                     KLIAPELQHLAWEVVR"
     gene            complement(1714..1953)
                     /gene="rpmB"
                     /locus_tag="VFI12_02310"
     CDS             complement(1714..1953)
                     /gene="rpmB"
                     /locus_tag="VFI12_02310"
                     /inference="COORDINATES: protein motif:HMM:NF013026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HET7055263.1"
                     /translation="MSRRCAISGKTTTFGRNVSFSKKRTNRRFEANIHYKNLYVPELG
                     VTIRCQLSTHALRTIDKKGLAAYLKSEGKTYRDLI"
     gene            complement(2299..>3231)
                     /locus_tag="VFI12_02315"
     CDS             complement(2299..>3231)
                     /locus_tag="VFI12_02315"
                     /inference="COORDINATES: protein motif:HMM:NF024045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HET7055264.1"
                     /translation="DLTRRLEPARAAVLAALAYLDASADFPDDEIPVSNVHHDLSHAV
                     DTLDAVLAGARSGKLLSEGATVALVGRPNVGKSSLLNALLRSDRAIVTPIAGTTRDIV
                     SERAVIEGIPVTLLDTAGIAESADVIERAGIERSRQAIARAAAIVMVVDGSVRPAGGD
                     FRIARDLAARFETGALGDIPLILAVNKADLGRHADQAVVVETLPGGVPAVPVSAKTGE
                     GLADLEAAISHVLRGDLSAAVQPSLLSARQHAELDRALLHLRTAFEALETGFPTDVLA
                     TDVRVAARALGNVTGEDIDESLLTEIFSRFCIGK"
BASE COUNT          580 a         1136 c          997 g          518 t
ORIGIN      
        1 agagcgaccg ccagagacga gtgaacacga aatacacctt ccgcgagggg gacttgtcag
       61 acgtgcgcgc aggtgcggca gcggccaaag atctcgagct gatggccctc gaccgcgaag
      121 ccggtcccgc tcagcgactg gccgattagc ggcgtcaggt cgcatccgcc gatcaccagc
      181 gtgtcgccgc actcgacgca gacgacctga tgatgcccgg gcaattgccg gaccagcgcg
      241 tggtcgcgat cggttcggtg gacgcgcgcc agccagccct gctcccgcag ccagaagagg
      301 aagcggtaga ccgttgccct gctccccgcg ccgagagact ggtcgatctc ggcgacggcc
      361 tcttcggccg taaacggcgc ctcccgcccg ccgatccagc gcaggacggc gacccgtggc
      421 tgcgtgatcc ggtacttctc gcgccggagt tggccgagcg tcgcctcgat ccaggccgga
      481 ccgggcacgc cgcgatgcgc gtgtgggctt gccgccgtgc aggcgatctc gctcaccgga
      541 ccacctccca cgccagatgc tgcagctccg gcgcgatcag cttctcccac gccagccgca
      601 ccgccgcatt cgagccgggc gtcgagatga ccagcttgcc gcgatatacc ccggccgtcg
      661 cccgggagag catcgcggcc gccccgactt gctcgtagct gagcatccgg aacagctcgc
      721 cgaagcccgg catcggcttc tcgatcttgc gggcaaggac gtcgtaggtt gtatcgcgcg
      781 gggcgatgcc ggtgccgccg ttgaacagga tcatccgcgc ctcggagtcg gcgaaacgat
      841 tcagcaccgc ctcgacctgg tccggctcgt ccttgatcag catgtacccg gaaacgaggt
      901 gcccgagcga ctcgatctcc ggcttcagat actgcatgtt ggtgtcggtc tcgggcgtgc
      961 ggctgtcgct gaccgtcacg atcgcgattg cgacctggcc gatctgctcg gccgcaattt
     1021 cccggtgcag atcggggctg agtttgcttt cggacgcgct catgccggtg tcgccttcgg
     1081 ccggggcgag cagcacgggc atccggccat gtgcggggca gaagtagccg tctgtcgcgc
     1141 gccgggatcg agcagcgact tcgccgccga cccgggcgaa tagaactcgg cgaccgagat
     1201 caccttggcc gcgcggttgc tcccgcaact cgggcaaacc gccggctcat cggctcgcgc
     1261 aaaggagcgc cgctcctcga acaacgtcgc gcagctcgga caccgaaaat cataagtagg
     1321 catagggcaa agacctccaa ccggtcactc gcccagatga tacccagtcg cgataccagc
     1381 cgtcaatgcg acccagtatc aagtcgcgat actcccaggc gcttggagag gcggagctcc
     1441 gtacccactt tggccctcac gaacgggtgc cttgtaatgc cgatccgcca cccgatcagc
     1501 ctcggagagg cttcgtgacc tcagcccggg tttcaacccc gggtgacccc gccaaatcac
     1561 ccgctatacg ccaacgggca tttcgggaaa gccaaggcgc gtacggagcg ccgccactac
     1621 ggttgagagc accatcgcgc cgaccgcaag atcaaacaaa acggcccggg gcaattgccc
     1681 cgggccgtcc gtcttctgga attgatgttc gcgctagatc aggtcgcggt aggtcttgcc
     1741 ttcggacttg aggtaggcgg ccagaccctt cttgtcgatc gtccgcagcg cgtgcgtgct
     1801 gagctggcaa cggatcgtca cgccgagttc cggcacgtac aggttcttgt aatggatatt
     1861 ggcctcgaag cggcggttgg tccgcttctt cgagaaggag acgttccgac caaaggtggt
     1921 cgtcttgccg ctgatggcgc atcgtcgaga catagcaaac gtcctttgat cggtcgatcg
     1981 gaatgcaggc gcacacaaaa atgcggtgac gacaccgcac aagggggtat tgtgccataa
     2041 cagaggcgcg gagtcgagga ggcgcgaagt cgggaagcag ggcgcgaaga cgcggaggcg
     2101 cggagttgta ggggcagatg gcatctgccc gccccgacgg ccggccattt caatccttcc
     2161 actgtcagcc tcggagaagt ttcgtattct cagcccgggg cttcaacccc aagcggtgcc
     2221 tcacgagcgc tcggtccgca agccgagtcc cgaaaaccga aaaccgaaat ccgaaccccg
     2281 accaacccca aaacccgttc acttcccgat gcaaaaccgc gagaagatct ccgtcagcag
     2341 cgactcgtcg atgtcctcgc ccgtgacgtt gccaagggcg cgagcagcaa cccgaacatc
     2401 ggtcgcgagg acgtcggtcg ggaagcccgt ctcgagcgcc tcgaacgccg tccgcaggtg
     2461 cagcagcgcc cggtccagct cggcatgctg ccgcgcgctg agcagcgacg gctggacggc
     2521 cgccgacagg tcgccacgta gtacatgcga gatcgccgct tccaggtccg ccagcccctc
     2581 gccagtcttc gccgacaccg gcactgccgg cacgccgccc ggcaatgtct ctaccaccac
     2641 tgcctgatcc gcgtgtctcc cgagatctgc cttattgacc gccagtatca gcggtatatc
     2701 gccgagcgcg ccggtttcga atcgcgccgc caaatcccgc gcgatccgga aatccccacc
     2761 ggctggccgc acgctgccgt cgaccaccat cacgatcgcc gccgctcgcg cgatcgcctg
     2821 ccgcgaccgt tcgatcccgg ctcgctcaat cacgtccgcg ctctccgcga tccccgccgt
     2881 gtcaagcaac gtgaccggga tcccttcgat caccgcccgc tcactaacga tgtcccgcgt
     2941 cgtccccgcg atcggcgtca cgatcgcccg gtccgaccgc aacaacgcgt tgagcaggct
     3001 gctcttgccg acgttcggcc gcccgaccag cgccacggtc gcgccctcgc tcagcaactt
     3061 cccgctccgt gccccggcca gcactgcatc aagcgtatcg accgcatgcg acaggtcgtg
     3121 atggacgttg ctgaccggaa tctcatcgtc ggggaagtcg gccgaggcat cgaggtaggc
     3181 cagagcagcc aggaccgccg cccgggccgg ctccagccgc cgggtcaggt c
//
LOCUS       DASSGX010000130         3001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_241713, whole genome shotgun sequence.
ACCESSION   DASSGX010000130 DASSGX010000000
VERSION     DASSGX010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241713"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="VFI12_02320"
     CDS             complement(<1..887)
                     /locus_tag="VFI12_02320"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7055265.1"
                     /translation="MTFPDDERILTSLHSADDLVQAGLNGRISRRGLIVRARDLGLAA
                     GVTAILLNATGLSARAADKALVTDGKKTKPAGKEVKDGQITVGVVGSIDTLNPYTTNL
                     YAQGFDVLSGVMDGLVGYDSRQRLKPVLAEGYSISDDGLTYTFKIRPNVAFQNGNLLT
                     AKDVVASWQMIMNKELPVWSRLGWDKIASIDTPDDRTAVVKTAQLFAPFLSSIAAGQF
                     TNCAICPASELEKGLDAFRERFDTKPIGTGPFRVKSVRQSEIILERYAKHWAGNPRLE
                     RITVRIYKDYAEQLAALAAG"
     gene            complement(1211..1813)
                     /locus_tag="VFI12_02325"
     CDS             complement(1211..1813)
                     /locus_tag="VFI12_02325"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HET7055266.1"
                     /translation="MRTLTRRGFMAGAMALIAPVAGSTPVFADQASDATDSIRTRTGP
                     SSPAQTDDSGRFVEERAHAAAPTEPSAEQKQAALGQTIVDFALSYYGYPYVAAGNSPA
                     GFDCSGFTQFVYLNVLGIDIGHGVEGQPAAGAWVDWGNWMPGDLIVFQNTYKAGISHV
                     AIYIGDGQIVHAENEGTGVTVSSIYSAYYSARYWGAVRLI"
     gene            2258..>3001
                     /locus_tag="VFI12_02330"
     CDS             2258..>3001
                     /locus_tag="VFI12_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055267.1"
                     /translation="MAYRFLLEVPESEFDEANIVVGSTGDAQVVVVRNSHGLGFDDPY
                     QDLTVAAHSLQVIDAIYDWYDSRPEPKPSLRLVLHSGQRLNLAEYDRGRMLAAIRYDQ
                     PWVERSLPKIGEHYREILPPGDTRIEGIAPQPMVHLDAVLVETNPIIAATRQLTFLEP
                     IHTMIKVPDLDRAERYYVDFLGVHLLGRERVDTAGDLELVERDYSGPRALAEGTEADF
                     SYLANEPVRLTLQRVGRGARLPQDLPNQIR"
BASE COUNT          520 a         1027 c          945 g          509 t
ORIGIN      
        1 ccggccgcca gcgccgcgag ttgctccgcg taatccttgt aaatgcggac cgtgattcgc
       61 tccaggcgcg ggttgccggc ccaatgcttg gcgtaccgct cgagaatgat ctcgctctgg
      121 cgcacgctct tgacccggaa cggcccggtc ccgatcggtt tggtgtcgaa ccgctcccgg
      181 aaggcgtcga gccccttctc cagctcgctg gccgggcaga tcgcgcaatt cgtgaattgg
      241 ccggcagcga tgctcgagag gaacggggcg aagagctggg ccgtcttgac cactgccgtc
      301 cgatcatccg gcgtgtcgat tgacgcgatc ttgtcccagc caaggcgcga ccagaccggc
      361 agctccttgt tcatgatcat ttgccaggag gcgacgacgt ccttcgcggt caggaggttg
      421 ccgttctgga aggccacgtt cggccggatc ttgaaggtat aggtcaggcc atcgtccgag
      481 atcgagtagc cctcggcgag caccggcttg agcctctgcc ggctgtcgta cccgaccaga
      541 ccatccatca cgccggagag gacatcgaat ccctgggcgt agaggttggt cgtatagggg
      601 ttgagcgtgt cgatcgagcc gaccacgccg accgtgatct ggccgtcctt gacttccttg
      661 ccagccggct tcgtcttctt gccgtcggtg accagcgcct tgtcagccgc ccgggccgag
      721 agccccgtcg cgttgagcag gatggcggtc acgccggcgg ccaggccgag gtcccgggcg
      781 cgaacgatca ggccgcgccg cgaaatacgg ccattcaacc cggcctgcac cagatcatcg
      841 gcggaatgga gtgaagtcag gatgcgctcg tcgtcaggaa atgtcaccag ccggtcctca
      901 gggaatgcac cacggtcatg ctgaatatgc ggggaaacgg gcgcggtgga ttgattgtac
      961 ccgctccgaa tcagtcgttg aaccacgcgc gacatcctgg atagattgtc ggcaatattg
     1021 ccccaggccc aggatggaga acgaacgccc acaacgtagc gcggggctgc ggcaccgcgc
     1081 tacgtgccgg ctcctacccg caagagagcc gctccggatg tttacgcccg ggcgatctcc
     1141 gggcaacgag gaaggggtgg gcgagcggtt cccagccgaa ctgcccaccc aaggagaaaa
     1201 gagccccggc ctaaatcagc ctgaccgcgc cccagtaccg cgcggagtaa taggcgctgt
     1261 agatgctcga gaccgtcacg cccgtcccct cgttctcggc gtggacgatc tgcccgtcgc
     1321 cgatgtagat cgccacgtgc gagatgccgg ccttgtaggt gttctggaag acgatcaggt
     1381 cgcccggcat ccagttcccc cagtcgaccc aggcgcccgc cgccggctgt ccctcgacgc
     1441 cgtgaccgat gtcaatcccg agcacgttca ggtagacaaa ctgcgtgaag cccgagcaat
     1501 cgaaccccgc cggcgagttc cccgcagcca cgtagggata cccgtaatag gaaagcgcga
     1561 agtcgacgat cgtctggccc agcgcggcct gcttctgctc agcagacggt tcggtcggag
     1621 ccgccgcatg cgctcgctcc tcgacgaagc gcccggagtc gtcagtctgc gcaggtgatg
     1681 atggtcccgt tcgggtccgg atggaatcgg tcgcgtcgga cgcttggtcc gcgaagacag
     1741 gggtgctccc agccactgga gcaatcaggg ccatggcgcc cgccatgaac ccgcgccgtg
     1801 taagcgtgcg cattgaagta cattccctcg agtaggtcgt gaagtcggcg gcccaggctc
     1861 gggcccggcc gcccaccatc ccgcagcccc gggatgggat atgtcgttag ctgtctgacc
     1921 ctcctcgcgc ctccgccaac ccgcagccag caggtcaacg cccgcgcttc cttcggcccg
     1981 cgccccgtcc atccacgaca ggcacggcaa tgcccggagc tgtcgcccaa cgtggcgaca
     2041 gcttgtgcaa acctgcatct tcagcgatga cggggcacac gctaccacga agtgccgccc
     2101 cgcaaccacc cctcaccggc acgatcgccc cggtactctg gtcccgtccg ccgtaccggc
     2161 acgttcgcga cgccccgcga ccggctgata caatcgccgc atccgggcgg gatgcgcatt
     2221 ccgcctgccg atcgcgttgc actgggagag agcgctcgtg gcctaccggt tcctgctgga
     2281 agtgccggaa tcagagtttg atgaggcgaa catcgtggtc ggctcgaccg gcgatgcgca
     2341 ggtcgtcgtg gtccggaact cgcatggtct cgggttcgac gatccctatc aggacctgac
     2401 ggtcgccgcg cactcgctgc aggtgatcga tgcgatctac gactggtacg actcgcgccc
     2461 ggagccgaag cccagcctgc gcctcgtgct gcacagcggc caacgcctca acctcgccga
     2521 atacgatcgc ggccgcatgc tcgccgcaat ccggtacgac cagccttggg tcgagcgctc
     2581 gctgcccaag atcggcgagc actaccgcga gatcctgcca ccgggagata cccgcatcga
     2641 ggggatcgcg ccccagccga tggtccacct cgacgccgtg ctggttgaga cgaatccgat
     2701 catcgccgcc accaggcaac ttaccttcct cgagccgatc cacacgatga tcaaggtccc
     2761 ggacctggat cgcgccgagc gctattatgt cgatttcctc ggcgttcacc tgctcgggcg
     2821 ggaacgggtc gacacggccg gcgatctgga gctggtcgag cgcgactatt ccggtccgcg
     2881 cgccctcgcc gagggcaccg aggccgattt cagctatttg gccaatgagc cggttcggtt
     2941 gacgctccag cgtgttgggc gcggcgcccg cctcccgcag gaccttccga accagatccg
     3001 g
//
LOCUS       DASSGX010000131         5645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1219721, whole genome shotgun sequence.
ACCESSION   DASSGX010000131 DASSGX010000000
VERSION     DASSGX010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5645
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1219721"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="VFI12_02335"
     CDS             complement(<1..932)
                     /locus_tag="VFI12_02335"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HET7055268.1"
                     /translation="MIALPTFAATPGGLSAKERDKATPTATETSTPEEEQTDPTATAT
                     SEPKEEKPVEKATSTNAAEPDKPAKSAKTTESQSTVASGPLAFPSTADAWAEEANPSA
                     NYGTTASVKVDAAPDPAKQGFMKFEVTGVSGRVTAAHLRLYVMAATATGPSIATVSND
                     WSETSLTWNARPPVTGAATEYPGAIAENAWVEFDVTNTVTANGVYSFVITGNSSDGVD
                     FSARERPDTAPQLVLTVDSSAGPVVSPTPLPPSNQVTPTTIAPTSTAAPKTATAAPAN
                     TLALRSLSVQSVIRAGGYGKITVCLSGVAPRNGAS"
     gene            complement(1186..1740)
                     /locus_tag="VFI12_02340"
     CDS             complement(1186..1740)
                     /locus_tag="VFI12_02340"
                     /inference="COORDINATES: protein motif:HMM:NF023043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HET7055269.1"
                     /translation="MAATVVASPGIVLAARQGLAGPKGVRYQESNGTPTSAETTPVTP
                     VATIEALQWTLSILSFQDPYTGEIRTPASPVPNMPALRFIAVQLLFENTSEEPLSLRT
                     SAFHLHDVDGFDYSTASGVSSPLQKFQSQTVQGGERARGWLFFAVPTGAELTELRMDE
                     PAAEMKVPLQGIANLPAPQATPQS"
     gene            1933..4176
                     /locus_tag="VFI12_02345"
     CDS             1933..4176
                     /locus_tag="VFI12_02345"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HET7055270.1"
                     /translation="MHRFGPDADSDVRDATTRRSLIRRLGYGLALPLIPGATHAAAAA
                     TAAGASVLVAARDSAEPIRSVADFACDGKSDDVEIQAALDSLGKYGGSVTLAPGTYSI
                     SATVNINTEGTVLVAPGALLSQSSNTSGTALVSVNRSNCLVTCVRFRGPRGESSGTGI
                     RLYGDTERGDAVNAVTIDKCRFDTLATAIEIGIVDQGSVGDCTISDVRIHRCGVGILS
                     RGFTNRIYSPFISNTDIGIHQTGDRASGSFQLYGATINNWNAQAIYLERGRDFVIDSL
                     WAERTLEMEPGAGPTEVVKIGSDQYEVANAHFTGVQSIHLPRGRSASDQMYVFYLAQS
                     RGLVVDAIRINDQAPTAVVFQGAAQTGRENRIRRVGFGPGDVPANWDTTRIQQASAKS
                     TGEVIVEQPADLAGSPPGAVLGTIPPGDFDYVVAQSESGAYYAKERNGNVAIFGLPLK
                     EVMNAILGSNRHIHFTPGTFSFGADHAVFVDLANLTLSGSGRDRTLIENDTDEDADTE
                     PFSFTRCTGVVVRDLTVSAAGMLRTTSDALDFDAGSDCLVERVDITRSRGRGIVFDGK
                     EPGAQAVRNVIRDCRITGCPGAGIELLVAEHCDVRDCHSFGNGEVGLKVNRHVEAGRN
                     SRFNRVSGGTYEANEGDGITVIDSDFTTIDGVRCLNNGRDGVRIHSFGGELTTVGTTV
                     TSSTCLNDLANPAPNRQVYGINIVSEDPANGVVSSVISGNYLEGNLQGGIADNGTRTV
                     IRNNVGQ"
     gene            complement(4190..4414)
                     /locus_tag="VFI12_02350"
     CDS             complement(4190..4414)
                     /locus_tag="VFI12_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055271.1"
                     /translation="MTFALVVLSCVIISAVCALLAWRLDPSPERGIWRRIWPAVYSGE
                     LAVTSAWMDENEATEEAAKFASESLNYVRG"
     gene            complement(4579..5235)
                     /locus_tag="VFI12_02355"
     CDS             complement(4579..5235)
                     /locus_tag="VFI12_02355"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7055272.1"
                     /translation="MIRVLIVDDHASAREPLAIMLGLEPDIEIVGQAGSVSEATAASA
                     EADLAIVDLGLPDGSGIGFIEQFRVRFPEAVVLVLTGETDLGVWAKALESGAAGVIPK
                     AAPFDEVLAAVRSAAAGEMVHSLAEITEMLRLAARHRIEDRQAERIISSLTPREIEVL
                     QALALGLSDKDIAGRLSVSPKTVRVHMANILEKLDRSSRLQALIFAVQHDIVSLTESK
                     "
     gene            complement(5232..>5645)
                     /locus_tag="VFI12_02360"
     CDS             complement(5232..>5645)
                     /locus_tag="VFI12_02360"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7055273.1"
                     /translation="AAFARTVADDRSIVVSGDQHVDVWADPARIGQVLRNLLVNASAY
                     SQTGTTITLRAERNGHLLSIQVQDEGRGIPEADLTRVFDKYVRGRRSDKATTPGLGLG
                     LYLSRRIVQAHGHELTVTSPAGCGAIFSFDLEIVE"
BASE COUNT         1074 a         1736 c         1738 g         1097 t
ORIGIN      
        1 gatgccccat tccgcggtgc gacgccagag aggcagacgg tgatcttgcc gtagccgcct
       61 gcccgaataa cggactgcac gctaaggctg cgcagggcga gcgtgttcgc cggggccgca
      121 gtcgcggtct tcggagcggc cgtcgaagtg ggcgcaatgg tggtcggcgt gacctgattg
      181 gacggcggaa gcggcgtcgg tgagacaaca gggccggccg aggaatcgac ggtcagcacc
      241 agctgcgggg cggtgtccgg ccgttcccgg gccgagaagt cgacgccgtc gctcgagttg
      301 ccggttatga caaatgagta gacgccgttt gccgtcaccg tgttggtgac atcgaactca
      361 acccacgcat tttccgcgat ggctccggga tactcggtgg ccgcgccggt aaccggtggc
      421 cgtgcattcc aggtgaggct ggtttctgac cagtcgttgc tgacagtcgc gatcgacggg
      481 ccggtcgcgg tcgcggccat gacgtagagc cgaaggtgcg cggctgtcac cctgccggag
      541 actcccgtga cctcgaactt catgaacccc tgtttggcgg ggtcaggagc cgcatccacc
      601 ttcacactcg cggtcgtccc gtaattggcc gagggattcg cctcttcggc ccaggcatcc
      661 gcggtcgacg gaaaagccag cggtccgctc gccaccgtcg attgtgactc agttgttttt
      721 gctgattttg caggcttgtc cggctctgca gcgtttgtgg atgtggcttt ctcgaccggt
      781 ttctcttctt tcggctcgga tgttgccgtc gcggtcgggt cggtctgctc ctcttcaggt
      841 gtcgatgttt cggtggcggt cggcgttgcc ttgtcgcgtt ccttggcgct cagcccgcca
      901 ggtgtagccg caaatgtcgg cagcgcgatc atcagcaaca gaattgccag cactcggtgg
      961 agtgcgggca acgtatgagt tgttcgcatg ggtgcccctt ctcctgttcc ctcaacaggc
     1021 gttattgcgt ccagccacgc aacgttcgcc aaggtaactg gttaaggggt ctactttcag
     1081 catgactgcc gtactgacta tttcctgcaa tcaccctgac ttcagtacta gcccgagctc
     1141 agagagattc agagaaatgg tcagattggg ccagattttg gacttctagc tttggggcgt
     1201 agcctgaggg gcgggcagat ttgcgatgcc ctggagcggc accttcatct cggcggccgg
     1261 ctcgtccatg cgaagttctg tcaactcagc accggtcgga acggcgaaga acaaccatcc
     1321 gcgcgcgcgc tcaccgcctt gcaccgtctg gctctggaac ttctgcaggg ggctgctgac
     1381 tcccgaggcg gtcgagtagt caaatccgtc cacgtcatgg aggtggaagg ccgacgtgcg
     1441 taaactcaat ggttcttcgg atgtattctc gaacagcaac tgcaccgcga tgaatcggag
     1501 tgcaggcatg ttcggtacag gcgaagcagg tgtccgtatt tcgccggtat acgggtcctg
     1561 gaaggacaaa atgctgagcg tccactgtaa cgcttcgatt gtcgccacgg gagtgacggg
     1621 tgtcgtttca gcggatgtcg gtgttccgtt gctctcctgg taccgtacgc ccttgggtcc
     1681 ggccaggcct tgcctggcgg ccagcacaat tccgggagaa gcaaccactg tcgctgccat
     1741 tacctgtcgc cgggaaaatc tgcggtccat agcatctccc acattgattg ttggccaacg
     1801 tgacgaagga ctggaacaag tttcaatgta ggagcaatat cgccaaaccg tcaactgggg
     1861 gtcagcttgt cccggcacat tccctggcca tcgccgcccg tcaactggac agaatctcgc
     1921 gctggagacg taatgcatcg ttttgggcct gacgcagata gtgatgttcg cgacgcgacc
     1981 acccggcgat ccctgatccg gcgccttggg tacggcctcg cccttccgct catcccgggc
     2041 gccactcatg cggccgctgc cgctaccgca gcgggcgcca gcgtgctggt cgcggctcgg
     2101 gattcagccg aaccgattcg gtcggtggcc gatttcgcct gtgacggcaa atccgatgat
     2161 gtcgaaattc aggcggccct ggacagcctt ggcaagtacg ggggatccgt gaccctcgct
     2221 cccgggacct attcgatttc ggcaacggtg aacatcaaca ccgagggaac ggttctggtt
     2281 gcgccgggcg cgctcctcag ccagtcgagc aatacatccg gcaccgcgct cgtctccgtc
     2341 aatcgcagca attgtctcgt cacttgcgta cggttccggg ggcctcgtgg cgaatcgagc
     2401 gggaccggaa ttcgcctcta cggcgatacc gagcgtggcg acgcggtcaa tgccgtgact
     2461 attgacaagt gccgcttcga cacgctcgcc actgcgatcg aaatcgggat cgtcgaccag
     2521 gggtcggtcg gcgattgcac gatctcggat gtccgcatcc accgatgtgg ggtcggcatc
     2581 ctgagccgcg gtttcacgaa tcggatttac agcccgttta tcagcaacac cgatatcggc
     2641 atccaccaga cgggcgatcg cgccagcggc tccttccagc tctacggcgc caccatcaac
     2701 aactggaatg cacaggcgat ctatctcgag cgcgggcggg atttcgtgat tgattcgctc
     2761 tgggcggaac gcaccctgga gatggagccg ggcgccggcc cgaccgaggt cgtcaagatc
     2821 ggatcggacc aatacgaagt agcgaacgcg cacttcaccg gggttcagag catccacctg
     2881 cccaggggcc gttccgcgtc cgaccagatg tatgtcttct acctcgcgca aagccgcgga
     2941 ctggtcgtcg atgcaatccg gatcaacgat caggcgccga ccgcggtcgt cttccaggga
     3001 gcagcccaga cgggtcgtga gaacaggatt cggcgtgtgg gctttggtcc cggggacgtg
     3061 ccagccaact gggatacgac ccgaatccag caggcgagcg ctaaatcgac aggcgaagtc
     3121 atcgttgagc agcccgcgga tctcgccggt tcgccgccgg gcgctgtgct gggcacgata
     3181 ccgccaggcg atttcgacta cgtcgttgca cagtcggaat ccggcgccta ttacgccaag
     3241 gaacgaaacg ggaacgtcgc catattcggc ctgccgctga aagaggtcat gaatgcaatt
     3301 ctgggcagta accggcacat ccatttcacg ccaggcacat tttcgtttgg cgccgatcac
     3361 gccgtcttcg ttgacctggc caacttgacg ctctccggct ccgggcgaga ccgcaccttg
     3421 attgagaacg acactgatga ggatgcggat accgagccgt tcagctttac gcgctgcacc
     3481 ggcgtcgtcg tgcgagacct gaccgtcagc gctgccggca tgctgcgcac aacaagcgac
     3541 gcgctggatt tcgacgccgg cagcgactgc ctcgtcgaac gagtcgacat cactcgcagc
     3601 cgggggcggg gaatcgtctt tgatggcaag gagccgggcg cgcaggcggt gcgcaacgtc
     3661 atccgtgact gccgcattac cggatgcccg ggcgccggca tcgaactttt ggtggccgag
     3721 cattgcgatg tccgcgactg ccactcattt ggcaatggag aagtcggctt gaaagtcaat
     3781 cgccacgtcg aggcgggccg aaactcacga ttcaaccggg tttcaggggg cacctacgag
     3841 gcgaacgaag gcgacggcat caccgtcatc gactcggact tcacgacgat cgacggcgtg
     3901 cgctgcctga ataatggccg ggacggtgtt cggattcact cgttcggggg cgagctcacg
     3961 accgtgggca cgaccgtcac aagcagcacc tgcctcaatg atctcgccaa tcccgcgcca
     4021 aatcgccagg tgtatgggat caacatcgtc agcgaagacc cggccaacgg agttgtgagc
     4081 tcggttatct caggaaatta tcttgaggga aacctgcagg gcggcattgc tgacaacggc
     4141 acgcgcacgg tgattcgcaa caacgtcggt cagtgaacgc ttcggccggt taccctcgaa
     4201 cgtaattgag ggattcgctc gcgaacttgg ccgcttcttc ggtggcttcg ttctcgtcca
     4261 tccaggcgct cgtgacggcg agttctcccg agtacacggc cggccaaatg cgacgccaga
     4321 tacctcgctc gggtgatggg tccaggcgcc aggcgagcag cgcgcacacg gccgagatga
     4381 ttacgcagga gagtacgacc aatgcaaacg tcattttggc ttgcgcctca ctgactcggc
     4441 ttcgaattcc cgatgacggg atatggtgcg ggaagcacca cactacacat aaatggtact
     4501 gcggtcaatc gcgcgcaaca gtggtcatcc ggctcaattc tgcataggcc atttgtccca
     4561 gaccatccgg aattattgtt acttggactc agtcaggctg acgatgtcat gctgcaccgc
     4621 gaaaatcagt gcttgcagac gcgaggagcg atcaagcttc tccaggatat tcgccatgtg
     4681 aacgcgaacc gttttgggcg agaccgaaag acgccccgcg atgtccttgt cgctcaaccc
     4741 gagcgccagt gcctgcagca cctcgatctc gcgaggcgtg aggctggaga tgatgcgctc
     4801 tgcctgccgg tcctcaatac gatgtcgggc ggcaaggcga agcatctcgg taatttccgc
     4861 gagcgagtgg accatttcac cggcggcggc cgaccgcacc gcagccagca cctcatcgaa
     4921 tggcgccgcc ttggggatca cgccggccgc gcccgattca agcgccttgg cccacacgcc
     4981 cagatccgtc tcacccgtca gcacgaggac aacagcctcc gggaaccgca cgcgaaactg
     5041 ctcgataaac ccgattccac tgccatcggg caagccgaga tcgacgatcg ccagatcggc
     5101 ctcggcgctc gccgccgttg cctcgctcac tgatcccgcc tgacccacaa tctcaatgtc
     5161 cggctcaagg ccgagcatga ttgccagcgg ttcccgggca gacgcgtggt catctacgat
     5221 cagaacgcga atcactcgac gatctccagg tcaaaggaaa agatggcgcc gcaccccgcc
     5281 ggagaggtta ccgtgagttc gtggccatgc gcctgaacga tacgccggga taagtagaga
     5341 cccaggccaa gtcccggcgt cgtcgccttg tcactccgtc gaccacggac gtacttgtcg
     5401 aacacgcggg tcaagtcggc ctccgggatt cccctgccct catcctgtac ctgaatcgaa
     5461 aggagatgtc cattgcgctc ggcccgaagc gtgatcgtcg tgcccgtttg ggagtaggca
     5521 gaggcgttga ccaggaggtt acggagaacc tgaccgatgc gagccggatc agcccaaacg
     5581 tccacatgtt ggtcgccgct gaccacaatc gagcggtcgt cggcgaccgt acgcgcgaac
     5641 gcggc
//
LOCUS       DASSGX010000132         2190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_241967, whole genome shotgun sequence.
ACCESSION   DASSGX010000132 DASSGX010000000
VERSION     DASSGX010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241967"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="VFI12_02365"
     CDS             <1..1020
                     /locus_tag="VFI12_02365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET7055274.1"
                     /translation="LSALANAARHGILFKGGAYLEAAGRIPIIAFDKTGTLTLGRPVL
                     TGAISLDPELTEDELIQMAASVERFSEHHIARAILAEANRRGLPLLPAEEFAARPGHG
                     VTARIVGRSCSVGNATMLRAMEISADIDHERARELLTCGKTVVFVTDEERVLGLIEIS
                     DKVRPESSSVIERLKRVGVKQIVMITGDNERVAAEVAREVGITDVRADVLPEAKMDVV
                     TELKRGGAVAMVGDGVNDAPALATADLGVAMGGAGTDVALETADMVLMTDDLNGVAYA
                     IELSRRTHRTIIQNITFSLGVIVVLAIAALTIGIPLPLGVVGHEGSTVIVVLNGLRLL
                     AWGRD"
     gene            complement(1145..1444)
                     /locus_tag="VFI12_02370"
     CDS             complement(1145..1444)
                     /locus_tag="VFI12_02370"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HET7055275.1"
                     /translation="MERRSLSITTPARKDLHSIRRYSTRMWGHNQSETYISQLLSAAE
                     TLCDFPERGWPVGNEPPNVRRVTAEYHIIYYRVLATEIEIIRILHQRMETPALLG"
     gene            complement(1444..1737)
                     /locus_tag="VFI12_02375"
     CDS             complement(1444..1737)
                     /locus_tag="VFI12_02375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HET7055276.1"
                     /translation="MNVSLTPDLENVIREKVDTGLYASASEVVREALRLMVEHDKRMK
                     ITAMRDAAIAEGIAAADRGDKVELTREVWDQIMAEADSMSDGEPLDLLVTGEF"
     gene            1893..>2190
                     /locus_tag="VFI12_02380"
     CDS             1893..>2190
                     /locus_tag="VFI12_02380"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HET7055277.1"
                     /translation="MPNRSWMIVGQAPAALGHERPAYAGPAGVKLRSWLTAAGLDGPD
                     PLEDNFYLTSVTKCFPGPGLSGKGDRMPSRAEVLLCAPHLEREIAVVQPEVVVTL"
BASE COUNT          373 a          645 c          746 g          426 t
ORIGIN      
        1 ctttcggcgc tggcgaacgc ggcccggcac ggcatcctgt tcaagggcgg agcgtacctc
       61 gaagcagccg gccggatccc gatcatcgcc ttcgacaaga ccggcacgct cacgctcgga
      121 cggccggtgc tgaccggtgc gatctcgctg gatccggagc tgaccgaaga cgaattgatc
      181 cagatggcgg ccagcgtcga gcgcttctcc gagcaccaca tcgcgcgggc gatcctggcc
      241 gaggcgaatc gacgggggct gccgctgctc ccggcggaag agtttgcggc ccggcccgga
      301 cacggcgtga cggcgcggat cgtcggacgt tcctgctcgg tcgggaacgc gaccatgctc
      361 cgggcgatgg agatctccgc ggatatcgat catgagcggg cgcgggagct actgacctgc
      421 ggcaagacgg tcgtcttcgt cacggacgag gagcgcgtgc tcggcttgat cgagatctcg
      481 gacaaggtcc ggcccgagtc gtcgtcggtg attgagcggt tgaagcgcgt cggcgtgaag
      541 cagatcgtga tgattaccgg cgacaacgag cgggtcgcgg cggaggtcgc gcgggaagtc
      601 gggatcaccg atgtgcgggc cgacgtgctg ccggaagcga aaatggacgt tgtcaccgag
      661 ctgaagcgcg ggggcgcggt ggcgatggtc ggggacggcg tgaacgacgc gccggcgctg
      721 gccacggccg acctgggcgt ggcaatgggc ggggccggca ccgatgtcgc gctggagacg
      781 gccgacatgg tgctgatgac ggacgacctg aacggtgtgg cctacgcgat cgagttgagc
      841 agacgcaccc accggacgat catccagaac atcacctttt cgctgggcgt gatcgttgtg
      901 ctggcgattg cggcgctgac gatcgggatt ccgctgccgc tgggcgtggt cggccacgag
      961 gggagcacgg tgatcgtcgt gctgaacggc ctgcgcctgc tggcctgggg ccgcgactaa
     1021 ccattcctgc ggaagcagac ctggtgggac cgatcttcct tgctcaattc atctcgctat
     1081 gcgtgcgaga gttggaggaa gtccagcgag agctcgcttg atttcttgcc cggagtccgc
     1141 tctgtcagcc gagcagtgca ggcgtttcca tgcgctgatg caggattcgg atgatttcga
     1201 tttccgtcgc caagacccgg taatagatga tgtgatattc agccgtgacc cggcgcacat
     1261 tcgggggttc gttgccgacc ggccaacccc gctcgggaaa atcgcagaga gtttcggcgg
     1321 ctgatagaag ttgtgaaatg taggtttctg actgattgtg cccccacatc ctggttgagt
     1381 agcgacgaat ggagtgcaaa tcttttcggg caggggtcgt tatgctgaga ctgcgtctct
     1441 ccactaaaac tcgcctgtaa ccaggagatc gagcggctcg ccgtcactca tcgaatccgc
     1501 ctccgccatg atctggtccc agacttcccg ggtgagttcc accttgtcgc ctctatccgc
     1561 cgcggcgatg ccttcggcga ttgcggcgtc gcgcatcgcc gtaatcttca ttcgcttgtc
     1621 atgttcgacc atgaggcgga gcgcctcgcg gacgacctca ctggcactgg cgtagagtcc
     1681 tgtatcaacc ttttcgcgga tcacgttctc gaggtcgggg gtcaagctga cgttcataat
     1741 gcatcctttt cttatcagtc cattcaggat gatagtagca agcaatggca aagattgata
     1801 ttccccttgc ggtgattgag tccgaattgg ccgcatgtaa tgcatgcgtt gggacggggc
     1861 atattgaagt tgctcggccg gtgttccggg gcatgccgaa ccggagctgg atgatcgtcg
     1921 ggcaggcgcc ggcggcgctc gggcatgagc ggccggccta tgcgggaccg gccggcgtca
     1981 agctccgctc gtggctgacg gccgccgggt tggacggacc agatccgctc gaggacaact
     2041 tctacctgac cagcgtcacc aagtgcttcc ccggtcccgg tctctccggc aagggcgacc
     2101 ggatgccctc ccgtgcagag gtcctgctct gcgcgccgca cctggaacgg gagattgccg
     2161 ttgtccagcc ggaggtcgtc gtcacgctcg
//
LOCUS       DASSGX010000133         2744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_350610, whole genome shotgun sequence.
ACCESSION   DASSGX010000133 DASSGX010000000
VERSION     DASSGX010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350610"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1221
                     /locus_tag="VFI12_02385"
     CDS             <1..1221
                     /locus_tag="VFI12_02385"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055278.1"
                     /translation="LYGLVPEGLPDTRPWLDHGNWEKSFPLGAGSATLEHLPYAFLPV
                     EGESLHQIAVGPVHAGIIEPGHFRLTANGETVVRLEQRLGYTHKGIEALMTGATLDKA
                     AKLAGRVSGDSTVAYAWAFAMAVESAVQLQVPTRAIWLRALMAELERLANHLGDIGAI
                     CNDAAFTLLHAHFGALRERVLRASDVAFGHRLMRDRVVPGGVSTELSPEGLAAIIAVV
                     GEIRRQMPRLVETYENTASLQDRTVGTGVALPTLVQQYGAGGYIGRASGRSFDARRIF
                     EYAPYERVVFEVPVLAEGDVDARVWIRIREVEQSLSLIDQILVQMPDGPLRAEIGSPN
                     RASEGMAIVEGFRGDVLVWLRIGAGGSVERCHLRDPSWFQWPLVKAAAEGNIVADFPL
                     CNKSFNCSYSGHDL"
     gene            1232..1819
                     /locus_tag="VFI12_02390"
     CDS             1232..1819
                     /locus_tag="VFI12_02390"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HET7055279.1"
                     /translation="MRRLLLDGMVRAPLTERPPLPGDAAIAELATALDRAAHRRLGRS
                     LSIRTVDAGSCNGCELEINAISNVFYDIERFGIRFVASPRHADVLTVTGPVTRNMADA
                     LERTYIAMPDPKWVVAIGDCARDGGCFASSYAVLGGAHHVVPIDLHIPGCPPSPTEIL
                     KGLLALMDRVNEKPSERQHRPPENGGHGAANAPRA"
     gene            1905..2186
                     /locus_tag="VFI12_02395"
     CDS             1905..2186
                     /locus_tag="VFI12_02395"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7055280.1"
                     /translation="MITAAQLRAARALIGIDQRTLASMAGVSLPTIQRMEASEGNVRG
                     IVDTLTKVIDALNAAGIELIGENGRSDTGGRGVRFRQARAPAARTAMKQ"
     gene            complement(2395..>2744)
                     /locus_tag="VFI12_02400"
     CDS             complement(2395..>2744)
                     /locus_tag="VFI12_02400"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HET7055281.1"
                     /translation="RSIAAVEGYHIHATDGEIGHVEDMLVEEADWSIRYLVVDTKNWW
                     PGKRVLLSPRSAHEVSCTDRTIDINVNREAVRSSPGYDATTEVDRDYEQRFNSYYGNA
                     APGSSARARASDL"
BASE COUNT          519 a          856 c          856 g          513 t
ORIGIN      
        1 ctttacggct tggttcccga aggtctccca gacacgcgac cttggctcga tcacggcaac
       61 tgggaaaaaa gctttcccct aggcgccgga tccgcaaccc tggagcactt gccgtatgca
      121 tttctgccgg tggagggcga aagtctgcat cagatcgctg tcggtccggt gcacgcgggc
      181 atcatcgagc cgggtcattt tcggcttacg gcgaacgggg aaaccgtggt ccgcctggag
      241 cagcgcctgg gctacaccca caaagggatc gaagccctga tgaccggcgc aactctcgac
      301 aaggcagcaa aactggccgg ccgagtctcc ggcgacagca ccgttgccta tgcgtgggcc
      361 ttcgccatgg ccgtcgagtc ggcggtacaa ctgcaagttc ccacgcgggc gatctggctg
      421 cgcgccctga tggcggaact ggagcgtctt gcgaaccatc tcggcgacat tggtgccatc
      481 tgcaacgacg cggcgttcac tctgctgcac gcacactttg gagctctgcg agaacgggtg
      541 ctccgcgcca gcgacgtcgc cttcggtcat cggctgatga gggaccgtgt ggtccccggc
      601 ggagtatcca ccgaattgag tccagagggg ctagcggcga tcatcgccgt cgttggcgaa
      661 attcggcgcc agatgccgcg tctggtcgag acgtacgaaa acacggcgtc gctgcaggat
      721 cgaaccgtgg gaactggggt tgccttgccg accctggtcc agcagtacgg cgccggcggc
      781 tatatcggcc gcgcgtccgg ccgaagtttc gatgcccgtc ggatcttcga atacgcgccc
      841 tatgagcgcg tggtcttcga ggtgccggtg ctggcagaag gcgacgtcga cgcacgtgtg
      901 tggattcgca tccgggaagt cgagcagagc ctctccctga tcgaccagat tcttgtccaa
      961 atgccggacg ggccgttgcg ggccgagatc ggcagtccta atcgcgccag cgagggaatg
     1021 gccatcgtcg agggctttcg cggcgacgtg ctggtttggc tgcgcatcgg cgccggcggc
     1081 agcgttgaac gctgtcacct gcgagacccg tcgtggtttc agtggcctct ggtgaaagcc
     1141 gcggcggaag gcaacatcgt ggccgatttt ccactctgca ataaatcctt caactgctcg
     1201 tattccgggc acgacctctg aggcgattgc catgcgcaga ctgctactcg atggcatggt
     1261 gcgagccccc ctcaccgaac gcccacccct cccaggggac gccgcgatcg ccgaacttgc
     1321 gacggcgctg gaccgcgccg cgcatcggcg gctggggcgc agtctctcga tcaggacggt
     1381 cgacgccggc tcctgtaacg gctgtgaact cgagatcaat gcgatcagca acgtcttcta
     1441 cgacatcgag cggttcggca tccgcttcgt cgcgtcgccc cgccacgccg atgtgctgac
     1501 ggtgaccggc ccggtcaccc ggaacatggc cgacgcgctc gagcgaacct acattgccat
     1561 gcccgaccca aaatgggtcg tcgccatcgg cgactgcgcc cgcgacggcg gatgctttgc
     1621 gagcagttat gcggtgctcg gcggcgctca ccatgtggtt ccgatcgacc tccacatacc
     1681 gggctgtccg ccgtcaccga cggaaatcct caagggcctg ctggcgctca tggaccgggt
     1741 caacgagaag ccttcggagc gacagcaccg accgccggaa aacggcgggc atggagccgc
     1801 aaatgcgccg cgggcttgag gatggaaaat gtttaaatta tgatgcttga tgcgtcatcg
     1861 tatgtatctt atacggtacc agacacttgt gcgagaggac cgtcatgatc actgcagcac
     1921 aattgcgcgc cgcccgcgct ctgattggaa tcgatcaaag aacgctggcc tccatggcgg
     1981 gggtgtcgct gccgaccatc caacgcatgg aggcgagcga aggcaatgtg cggggcatcg
     2041 tggacaccct gacgaaggtc atcgacgctt tgaacgcggc cggtatcgag ctcatcggcg
     2101 aaaacggtcg aagcgatacc ggcgggcgcg gtgtgcgctt tcgccaggct cgagcgcccg
     2161 cggcccggac cgcaatgaag caatgatccg gttgacgata agatgaagtc tcgtgttcca
     2221 taactgccca gatagccatc tgggaaggca agaacaaggt tctattgcca acgaccgctc
     2281 tcctcggtca cgatcccagc acccgtgtca tcggcccgca tcgatcgaga gacttctacg
     2341 gacccctcgg acagtcgcct gaccgatctg gccagccgaa aaacagcatg acagtcatag
     2401 atcagacgct ctggcacggg cggatgaacc cggcgcggca tttccataat aggaattgaa
     2461 ccgttgctcg tagtcccggt cgacttccgt cgtggcatcg taaccagggc tgcttctgac
     2521 cgcttcgcgg ttcacgttga tgtcgatggt tctatccgtg cagctgacct catgcgcgga
     2581 gcgcggggaa agcaggaccc tcttgccggg ccaccaattc ttggtgtcta cgaccaagta
     2641 gcgaatgctc cagtcggcct cttcgaccag catgtcctcg acatggccaa tttcgccgtc
     2701 tgtggcatgg atgtgataac cctcgaccgc ggcaatgctg cgca
//
LOCUS       DASSGX010000134         2914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1439435, whole genome shotgun sequence.
ACCESSION   DASSGX010000134 DASSGX010000000
VERSION     DASSGX010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1439435"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VFI12_02405"
     CDS             <1..187
                     /locus_tag="VFI12_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055282.1"
                     /translation="VGCDAPSDLPIAVFLRDLDVTGKIEGREQDGRSPATAFPAIDWR
                     ARPGPRWRRVIDRHGRD"
     gene            complement(276..1163)
                     /locus_tag="VFI12_02410"
     CDS             complement(276..1163)
                     /locus_tag="VFI12_02410"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HET7055283.1"
                     /translation="MRPQVISVPYRYDRFEEGLGLGPSRLLEIGLAERAAAVHQAILE
                     DADRDDDRTAVNIGILGRSTAALVAQARTTGDPVLVVAGDDTAAIGVVAGLQASDGAS
                     RALGVVWIDAHGDFNTPDTSYSGILAGMPLAVLAGLAGPRWREAAGLEAPVPVERMVL
                     VGARDLDKAEEELIRRHNLQRVSVQDVRKEEGFATPLARLAATCESLYVNIDMDVLNP
                     HLTPSATTQSEGGFELDELGAVLRAALKTGLVSAVTLTSLNPLGGARGQVSTRSAWQI
                     IQTILTEWDTVPAPPADVS"
     gene            1199..1789
                     /locus_tag="VFI12_02415"
     CDS             1199..1789
                     /locus_tag="VFI12_02415"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HET7055284.1"
                     /translation="MHSTERIHIGARDLRTDGRTITTGDSDPYAVTGDLLSAPPNTQT
                     VDYYKLLGVPYSATSREITRAYRNAMKRTHPDRVVAADRPRAEERAKLLNLAWRTLTR
                     PDERAKYDQTLRNELVQDQIMSRYFGAMGMPGQPNDLYGEALRREHTEFERKERRQSD
                     RGATLTIFLIFAAATALVVCLIVVAAVVSALAHALL"
     gene            complement(1719..2873)
                     /locus_tag="VFI12_02420"
     CDS             complement(1719..2873)
                     /locus_tag="VFI12_02420"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET7055285.1"
                     /translation="MRKTRSAPAPMPVVAPSPLLAGVAEQFPGAGRPVSVAGRPQLVQ
                     FEADGQGWLVHGLSSDQTESLISLRHQFLAHPAIAGTGIAPVPHPGHSLGQVDGAPYE
                     VVSFLPGKSWAMNGRLMLGGHGLHRPLPPPPGGLSALSAQIARFHLASQDAPQGLLPR
                     LTVRDFQRAQRDRWQYVRDRIVESGVREAHIQRWLSASQRVIYASWDVLEAVAFLEPI
                     PLVATHGNLWPTHVVADNETVRVIDWRTARYSSPLTDVAQLIARFNGWSGASLEEVVE
                     SYMGARTLTADERRLLPVFGALDLAVETGILLAEAYVADIDQTSHYAELARSGSGQML
                     ASLESIGWSIRKTNLDARELRREAKENARAVRAPGQKPRPRRLSSKRPKQ"
BASE COUNT          497 a          949 c          989 g          479 t
ORIGIN      
        1 agttggttgc gatgcgccca gcgacctgcc aatagcggtc ttcctgcgcg atctcgacgt
       61 gaccggcaag atcgaaggtc gcgagcagga cgggcggtcg ccggcaaccg cattcccagc
      121 gatagattgg cgggcgcggc ctggcccacg atggcgtagg gtgatcgatc ggcatgggcg
      181 agactgacca acgtaatccg gaaaagaaca catgttcgta ttccggattc tagagcgtca
      241 ggcgccgtgt ggcaatgggt tggtgagctg ttcggctacg agacgtcagc cggcggggcg
      301 ggcacggtgt cccactctgt gaggatggtc tggatgatct gccacgccga ccgcgtcgac
      361 acctgaccac gtgcgccgcc gagcgggttc aggctggtca acgtgaccgc cgagaccaga
      421 ccggtcttga gcgccgcccg cagcacggcg cccagttcgt ccaattcgaa gccgccttcc
      481 gactgcgtcg ttgcgctcgg ggtgaggtgc gggttcagaa cgtccatgtc gatattgacg
      541 tagagcgatt cgcaggtggc ggcaaggcga gcgagcgggg tcgcgaatcc ttcttccttg
      601 cgcacgtcct gaacgctgac tcgctgcagg ttgtgcctcc ggatcagctc ctcttcggcc
      661 ttgtcgaggt cccgagcccc gaccagcacc atccgttcga ccggcaccgg cgcttccagg
      721 ccggcggcct cccgccaacg gggaccggcg aggcccgcga gcaccgccag cggcatgccc
      781 gccagaattc cgctgtagct ggtatcgggc gtattgaaat cgccgtgggc gtcgatccag
      841 acgacgccga gcgcgcggct ggccccgtcc gatgcctgca aacctgcgac gacgccgatc
      901 gcggccgtat cgtcgcccgc aacaacaagc actggatcgc cggtggtgcg tgcctgggcg
      961 accaaggcgg cggtcgaacg gccgaggatg ccgatgttga ccgccgtgcg gtcatcatca
     1021 cggtcggcat cttccagaat ggcttggtga acggcggcgg cgcgctcggc aaggccgatc
     1081 tcgaggagcc ggctcggccc aagccccagc ccttcctcaa agcggtcgta gcgataaggt
     1141 accgagatca cctgtggtcg catcggctgg gccttcccgg gtcgtgagca gaatggatgt
     1201 gcattctacc gaacggattc acatcggcgc gcgggacctg cgcacggacg ggcgtacaat
     1261 cacgactggc gattccgacc cctacgctgt caccggtgat ctcttgtccg caccgccgaa
     1321 cacccagacc gtcgattact acaagcttct cggggtaccg tattcggcga cgtcccggga
     1381 aatcacccgc gcctaccgga atgccatgaa gcgcacccat cccgaccggg tcgtcgcggc
     1441 cgatcgacca cgggcggagg agcgggcgaa actgctcaat ctcgcctggc ggactctgac
     1501 caggccggat gagcgcgcga agtacgacca gacgctgcgc aacgagctgg tccaggacca
     1561 gatcatgtcg cgctatttcg gtgccatggg aatgcccggc cagccgaatg acctgtacgg
     1621 cgaggcgctg cggcgagagc acacggagtt cgagcgaaag gaacggcggc agtcagatcg
     1681 cggcgcgacg ctgacgattt tcctgatatt tgcggcggct actgctttgg tcgtttgctt
     1741 gatagtcgtc gcggccgtgg tttctgccct ggcgcacgca ctgctctagc gttctccttc
     1801 gcctcgcggc gcaattcgcg cgcgtccagg ttggtcttgc ggatcgacca gccgatcgac
     1861 tccagcgagg cgagcatttg gccggaaccc gaccgggcaa gctcggcata atggctcgtc
     1921 tggtcgatat ccgcgacgta ggcctcggcg agcaggatcc cggtttcgac ggcgaggtcc
     1981 agcgcgccga agaccggaag caggcgccgc tcatccgcgg tcaacgtgcg ggcgcccatg
     2041 taggactcga cgacctcttc gagcgacgct ccgctccagc cgttgaaccg ggcaatcagt
     2101 tgcgccacgt cggtcagtgg gctcgagtac cgcgccgttc gccagtcgat aacgcgcacg
     2161 gtctcgttgt cggccacgac gtgggtcggc cagaggttgc cgtgggttgc cacgagtgga
     2221 atcggttcca gaaacgcgac cgcctccagg acatcccacg aggcatagat cacgcgctga
     2281 ctggcgctga gccaccgttg aatatgggcc tcgcggacgc ccgattcgac gatccggtcg
     2341 cggacgtatt gccagcggtc gcgctgcgcg cgctggaagt cgcggacggt cagccgcggc
     2401 agcaagccct gtggagcatc ctggctggcc aggtggaaac gggcgatctg cgccgacagg
     2461 gcggacaggc cgccgggcgg aggtggcaac ggccggtgca gtccgtgacc accgagcatc
     2521 aaccgaccgt tcatcgccca cgacttgccc ggcaggaagc tgacgacctc gtatggcgcg
     2581 ccgtccacct gcccgagcga gtgacccggg tgcggcaccg gcgcgatgcc ggtcccggcg
     2641 attgccgggt gcgccaggaa ttggtgccgg agcgaaatca gcgattcggt ctggtcgctg
     2701 gagagaccgt ggaccagcca gccctggccg tccgcctcga actgcacgag ctgcggccgg
     2761 ccggccaccg agacaggtcg gccggcgccg gggaattgct cggcgacgcc cgccaacaag
     2821 ggcgaaggcg ccacgactgg cattggagct ggcgctgagc gggtcttgcg catgatctac
     2881 tcgtcgtctg ccggcgccct ggacgcgctc tgga
//
LOCUS       DASSGX010000135         2150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1220953, whole genome shotgun sequence.
ACCESSION   DASSGX010000135 DASSGX010000000
VERSION     DASSGX010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1220953"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="VFI12_02425"
     CDS             <1..940
                     /locus_tag="VFI12_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055286.1"
                     /translation="NRQIVSLIRDGLYIASGAKSGAEDAQVMAASERLGLPGLLRVAS
                     AFADTDSGIRSAVIPQLPLEMAVLEAILAPASVPVSDGSRAAAARSHNAEPIPASRPA
                     SVDSRPAASSTSPESASEPAAEDRQSTRLQDMIRKPASARGVRESPPVAGNAIERAAK
                     SNDGPSLTSGAAASIETLVGLWPKIRADVKSLDRRTEALLMEIDPVDVTAEEVVLAAA
                     YEFHRDKVNEDERRAIIETVIGRLLERPIRVSCVLRAEFVPSGAKPKREAPPVVAPVA
                     EMEATPDAQAGTDEERADEKMLGAVVRMFDGEIVEE"
     gene            complement(948..>2150)
                     /locus_tag="VFI12_02430"
     CDS             complement(948..>2150)
                     /locus_tag="VFI12_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055287.1"
                     /translation="NAQLPTETPGPTSTPAPIDRQPGETVAMATPSEDLFKALGSAAA
                     LASGVDRVTVVQLDSGKATDLPVRRSSSILAVVAPDGSRCLVIDRSGGRVSVRMYGAN
                     GTETASWSPKSHAATPVAFPIASPVAGTSSIMTGDRIAWKRDGSGAVIAVAGVGVFVA
                     DAKLKMTPLKVGQPHAVTAVAWSPSEQSIAVGIWDGERRAASIVTISVQSPNSEGTPV
                     LALPDGDGRYVRSLAWGTERVGLVFALRAASTNFSLPNDLYYLPRFGQPMRLLASAGV
                     AAPAAVIDQIAVAGNGTTVAFTVLIPGEVGLRFHSVWVTDAVAPGLIQADTSGLRRVT
                     DVVWTTVGLTVSGTRRTQQDGAAYQVAVVDRLTADASRQISADRSAATPVASPVASPA
                     PATPVESR"
BASE COUNT          389 a          716 c          710 g          335 t
ORIGIN      
        1 caatcgccag atcgtctcat tgatccggga tgggctctac attgccagcg gggccaaatc
       61 gggcgccgaa gatgcccagg tcatggcggc aagcgagcgc ctcgggcttc ccgggctgct
      121 ccgcgtggcg tccgcctttg cggataccga cagcgggata cgctcggccg tcatccccca
      181 gctccccctc gaaatggccg tgctggaggc gatccttgcg ccggcctcgg tgccggtgag
      241 cgatgggtcg agggccgctg cggctcgaag ccacaacgcc gaaccgattc cagcgagtcg
      301 tccggcttcc gtggattcga ggccagcagc cagttcaacc tcgcctgaat ctgccagcga
      361 gcctgctgct gaagatcggc agagcactcg gctccaggac atgatccgga aacccgcctc
      421 ggcacgagga gtgcgtgaat cgcccccagt ggccggcaac gcgatcgaac gcgctgcgaa
      481 atccaatgat gggccatcgc tcacatcggg cgccgccgcc tcgatcgaga ccctggtcgg
      541 gctctggccg aagatccggg ccgacgtcaa gtcgctcgat cggcgcaccg aagcgctgct
      601 gatggagatc gatcccgtcg acgtgacagc cgaggaagtg gtgcttgctg ccgcctacga
      661 attccatcgc gacaaggtca acgaggacga gcgccgggcc atcatcgaaa cggtcatcgg
      721 ccggctcctc gagcgcccga tccgcgtctc atgcgtcctg cgcgcggagt tcgtgccgtc
      781 aggggccaag ccgaagcgag aggcaccacc ggtggtcgcg ccggtcgccg agatggaagc
      841 gacacccgac gctcaggctg ggaccgacga agagcgcgct gacgagaaga tgctcggcgc
      901 cgtggtccgg atgttcgacg gagagatcgt cgaggagtaa cggccgccta gcgcgactcg
      961 accggtgtgg ctggagccgg actcgcaacc ggggacgcga ctggagttgc ggcgctgcga
     1021 tcggcactga tctgcctaga tgcatccgcg gtcaggcgat cgaccactgc gacctgatat
     1081 gcggcgccat cctgctgcgt ccggcgggtg ccggatacgg tgagccccac ggtcgtccac
     1141 acgacatcgg tcacgcggcg cagcccgctc gtatccgcct gaatcagccc gggtgcgact
     1201 gcatcggtca cccaaacgct atggaaccgg agcccgacct ctcccggtat gagcacggtg
     1261 aaggcgactg tcgtaccgtt cccggcgacg gcaatctgat cgatgactgc cgcgggcgct
     1321 gccacaccgg ccgacgcgag caatcgcatc ggttggccaa accgcgggag gtagtacagg
     1381 tcgttcggca gcgagaagtt cgtgcttgcc gcgcgcaggg caaagacgag gccgacgcgc
     1441 tccgttcccc acgccagcga gcggacatag cgaccatccc catccggcag cgcaagtacc
     1501 ggggttcctt cgctattcgg cgattgcaca ctgatggtca cgatgctggc ggcccgtcgc
     1561 tcgccatccc agatacccac cgcgatcgac tgctctgacg gtgaccaggc gacggccgtg
     1621 actgcatgcg gctgaccaac cttgagcgga gtcatcttca acttcgcatc cgcaacgaag
     1681 acgccaacgc ccgcaaccgc gatcaccgcg cctgaaccat ctcgcttcca ggcgatgcga
     1741 tcgccggtca tgatcgacga cgttccggcc actgggctgg cgatcgggaa cgccactggt
     1801 gtcgccgcgt gtgatttggg cgaccaagac gcggtctccg taccattagc gccgtacatt
     1861 cgaaccgaaa cgcgtccgcc ggatcggtcg atgaccaggc agcgcgatcc gtcgggcgcg
     1921 acgacggcga ggatcgaaga ggagcgccgc acgggaagat ccgtcgcctt gccggaatcg
     1981 agctgcacga cagtcacgcg gtcgacgccc gatgcgaggg ccgcggcaga gcccaacgcc
     2041 ttgaaaagat cttcggatgg cgtggccatt gccacagtct cgcccggctg ccggtcgatg
     2101 ggggcggggg tacttgtcgg ccccggagtt tcggtcggca attgggcatt
//
LOCUS       DASSGX010000136         3267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1004551, whole genome shotgun sequence.
ACCESSION   DASSGX010000136 DASSGX010000000
VERSION     DASSGX010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004551"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..1121)
                     /locus_tag="VFI12_02435"
     CDS             complement(78..1121)
                     /locus_tag="VFI12_02435"
                     /inference="COORDINATES: protein motif:HMM:NF024919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HET7055288.1"
                     /translation="MRVWRKALAGAFAVGLLGAQLGFAGVARAADSSGVDSWIELSGT
                     KPASGCTIDASIEVRSGGYVQPGVDVFIGMYVGTNLISSDEVTTDDNGIAFLTIDPSA
                     AEGQDAWIDVTLNDTYWWGTAAYPGSGDTCYSGSLKSTKSGPIADAPTAGNSTSAPTN
                     GSDVDTSTGAVIIPEVGFYVQQRNLSCEYAAVYIATAAWGNGISEYATEATVGWSDNP
                     HLGYRGNINGWWGNTNDYGVYAEPLSWALAQYGFVGEVAYTHGDPAWLINEINAGNPT
                     IVWLGMWGDQSVYEGGYRVVAGMHVMVAYGYDDGGVYLSDPASGGSRYYDWGTFEWMW
                     GVMDGMSLSVYPG"
     gene            1509..2009
                     /locus_tag="VFI12_02440"
     CDS             1509..2009
                     /locus_tag="VFI12_02440"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HET7055289.1"
                     /translation="MSRLISTTDRRLIRLLQQNARVSFAELSRVTGIPESTVRRRVER
                     LQERGIIRFAMIADADQLGYDISAMVGLRIDLAKLHQISNILNGMPEVVFASFLTGSF
                     DILAQVVVESQDALVELLTRLASTEGVRSSETFLMPRVLKPLTAWVIPEPDDDGSERG
                     VDFSQE"
     gene            complement(1996..3228)
                     /locus_tag="VFI12_02445"
     CDS             complement(1996..3228)
                     /locus_tag="VFI12_02445"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7055290.1"
                     /translation="MSDADPQQFDIVVIGGSVAGSAVAATLSDEGYSVALIEREAAFR
                     DRARGEGIHPWGMDEAEELGLLELLRAGGAHPLPVWLTYVDRVPVEPLLMAEHSARGH
                     VEQGVFHPTLQETMLVYAREHRVSVYRPASLVALDRRDDRVFVGVEQDGETISLAAQI
                     VIGADGTHSKTRSQVGIATEKDVLHHWFAGVLIDGFGGDPDAAHSSLVAGGRFFILPQ
                     GNGRARAYLALMPVRIAAIQADRSGRALLQLIAEYLPDGMLADAEPAGPQGIFSNADI
                     WPETAAADRIVLIGDAAGTNDPSVGNGIAMALRDVRELRDAIRESGLTQTALELYALR
                     RARYYGALRQYASWMGEIWLEEGPEADAKRVRFRTAREMDPDAGGFNMITMLGPRDLV
                     PTEAARARFFGEAAPTPG"
BASE COUNT          579 a         1125 c         1008 g          555 t
ORIGIN      
        1 gattcggtcc cgatcgcgca atcgaaaacg gccggtgagc gctcaccggc cgttctatcg
       61 tcagttgtgg tcgaggatca gcccgggtag accgagaggg acatgccgtc catgacgccc
      121 cacatccact cgaacgtgcc ccagtcgtag tagcgcgagc cgccgctggc cgggtctgag
      181 agatagacgc cgccatcgtc gtagccgtag gcaaccatca cgtgcatgcc ggccacgacc
      241 cggtagccac cctcgtagac gctctggtcg ccccacatgc cgagccaaac gatggtcggg
      301 ttgcccgcgt tgatctcatt gatcagccag gccggatcgc catgcgtgta ggcgacctca
      361 ccgacgaatc catattgcgc gagggcccag gagagcggct cagcgtagac gccgtagtcg
      421 ttggtgttgc cccaccagcc gttgatgttg ccccgatagc cgaggtgcgg attatcggac
      481 cagccgacgg tggcctcggt ggcgtactcg gatattccgt tgccccaggc agccgtggcg
      541 atgtagacgg ccgcgtactc gcagctcaag ttccgctgct gcacgtagaa accgacctcg
      601 ggaatgatca ccgcgccggt cgaggtgtcg acatcggaac cgttggtcgg cgcgctcgtc
      661 gagttacccg cggtcggcgc atccgcaatc gggccggatt tcgtcgactt cagtgagccg
      721 gagtagcagg tatcgccgct gcccgggtag gccgcggtgc cccaccagta ggtgtcgttc
      781 agggtgacgt cgatccaggc gtcctgaccc tcagcggcgc tcgggtcgat cgtgaggaac
      841 gcgatgccat tgtcgtcggt cgtaacttcg tccgagctga tcaggttcgt cccgacgtac
      901 atgccgatga agacatcgac acccggttgg acatacccac cactccggac ctcgatcgag
      961 gcgtcgatcg tacatccgga tgccggtttg gttccgctca gttcaatcca ggagtctacg
     1021 cccgaactat ccgccgcgcg tgccacgccc gcgaacccga gttgggcgcc cagcaaccca
     1081 acggcgaatg caccagccaa cgccttacgc caaaccctca tgaacctccc ccaactcgct
     1141 tgatcgccgt gccgccacca gtagcaccag tcagtagtac gccaactact tggtacaggt
     1201 tacgcgcctg cgcgccgatg cggatgcacc ggagccttca ggcgggcggg ctgaccatcc
     1261 gaactgtagc atagaaatac cccgtcagtg cctgtaagtc gacgaatctg ggcaacgttt
     1321 cctgccggat cggcgatccc cgccactctc ccgtcacccg gtcctatcca cacgtcccct
     1381 tccccgcgta gagtcatcat ggtagcttga ctcgcacacg ccgatcgcac tccggcggcg
     1441 ctcgcgaatc tacctgcgcg ctcgacaatg cgaggcctgg cggacatgga aggacggtta
     1501 cacccatcat gtcgcggctg atttcgacaa ccgaccggcg attgattcgc ctgctgcagc
     1561 agaacgcgcg cgtctccttt gccgagctca gccgggtgac cgggattccg gaatcgacgg
     1621 tccgccgccg tgtcgagcgc ctgcaggagc gcggcatcat tcgtttcgcg atgatcgccg
     1681 acgccgatca gctcggctac gacatcagcg cgatggtcgg gctccgcatc gacctcgcca
     1741 agctccacca gatcagcaac atcctgaacg ggatgcctga ggtggtattc gcctcgttcc
     1801 tgaccggcag cttcgacatt ctggcgcagg tcgtggtcga gtcgcaggat gccctcgtcg
     1861 agctgctgac caggttggcc agcaccgagg gcgtgcgctc gtccgagaca ttcctgatgc
     1921 cgcgcgtgct taagccgctc acggcctggg tgatcccgga gccggacgac gacggcagcg
     1981 agcgcggcgt cgatttcagc caggagtagg cgccgcttcc ccaaagaatc gcgcccgggc
     2041 cgcctcagtt ggcacaagat ctcgcggacc gagcattgtg atcatgttga atccgccggc
     2101 atcagggtcc atctcacgag cagtccgaaa gcggacccgc ttcgcatccg cctcgggacc
     2161 ttcctcgagc cagatctctc ccatccagct cgcgtactgt cgcaaggcgc cgtagtaccg
     2221 cgcccggcga agcgcgtaga gttcgagcgc ggtttgggtc aagccggact cgcgaatcgc
     2281 atcccgcagc tcgcggacgt cgcgcagcgc catcgcgatc ccgttgccga ccgacgggtc
     2341 gttggtcccg gccgcatccc cgatcaggac gattcgatcc gcggcggccg tctccggcca
     2401 gatatcggcg ttgctgaaga tgccctgtgg tccggcaggt tcggcgtcgg cgagcattcc
     2461 atctggtaga tactcggcga tcagttgcag caacgcccgg ccggagcgat ccgcctggat
     2521 cgccgcgatg cggaccggca tcagcgccag gtaggcgcgg gctcgcccgt tgccctgcgg
     2581 gaggatgaaa aagcggccgc ccgcgacgag cgacgagtgc gccgcgtccg gatcaccgcc
     2641 gaacccatcg atcagcacgc cggcgaacca gtggtgcagt acgtccttct ccgtcgcgat
     2701 cccgacctgg gatcgcgtct tcgaatgggt gccgtccgcc ccgatcacga tctgcgccgc
     2761 gagcgaaatc gtctcgccat cctgctcgac gccgacgaag acgcgatcat cccggcggtc
     2821 gagtgcgacc agcgacgccg gccggtagac cgatacccgg tgctcccgcg cgtataccag
     2881 catcgtctcc tgcagggtcg ggtggaagac gccctgctcg acatggccgc gcgctgagtg
     2941 ctcggccatc agcaacggct cgaccggcac acgatcgacg taggtcagcc agaccggtaa
     3001 cgggtgcgcg ccgcccgccc ggagcagctc cagcaagccg agttcttcag cttcgtccat
     3061 cccccacgga tggatcccct cgccacgagc ccgatcgcgg aaggcggcct cgcgctcgat
     3121 cagggcgacc gagtacccct cgtccgagag cgtggcggca acggccgaac cggcaacgct
     3181 gccgccgatg acaacgatat cgaattgctg cgggtcagcg tcagacacgt cgcgctagcg
     3241 ggactcggca atcgcatagg ccacgag
//
LOCUS       DASSGX010000137         2272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_678310, whole genome shotgun sequence.
ACCESSION   DASSGX010000137 DASSGX010000000
VERSION     DASSGX010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678310"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VFI12_02450"
     CDS             complement(<1..488)
                     /locus_tag="VFI12_02450"
                     /inference="COORDINATES: protein motif:HMM:NF016825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HET7055291.1"
                     /translation="METNAPDNRTIHDYLSALRSGSPTPGGGSAAAYAGAMGASLAAM
                     VARLTLRAPEADTALAPAAKRLDALVDELSASARDDEDCYGRYRAAAALPKATAGEKQ
                     ARSAAMQAALQAAAETPLLTARRTLEALDLTAIVAELGTRYALSDVHVAGHLLTAAIE
                     GAL"
     gene            complement(516..875)
                     /locus_tag="VFI12_02455"
     CDS             complement(516..875)
                     /locus_tag="VFI12_02455"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HET7055292.1"
                     /translation="MGNQRSIVRNVRNGQIVGDDIEEAGGLWGSFKGLMLRKSIPDGY
                     GMVFRPARGIHTNFMRFPIDLIYFDKDNRVTKVRPAMKAWRWDFTNAAGVIEMLPDSA
                     ARADVRVGDQLRFEGRA"
     gene            complement(962..1420)
                     /locus_tag="VFI12_02460"
     CDS             complement(962..1420)
                     /locus_tag="VFI12_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055293.1"
                     /translation="MSKQRADSSKTYLWCDHCRRSFRHEEAIDGACPVCGSPMQPTGK
                     MNAILRGLMANELAASPIVTKHRQIVRLIWSRNGQGEEYYRLLQPKMPYNRFEARVTE
                     LICRGAEEGWISVVLPPAPTADERNYRIEFHDENRFLAELDAIAAETTKR"
     gene            complement(1493..1729)
                     /locus_tag="VFI12_02465"
     CDS             complement(1493..1729)
                     /locus_tag="VFI12_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055294.1"
                     /translation="MGDIIRSIGDFRIDNLANYGVLSDTIEVFLAVAMVLFGVYLTHR
                     IQVWADKMGGEHPGAVNRGFGGGANRYEEHGIRE"
     gene            complement(1744..>2272)
                     /locus_tag="VFI12_02470"
     CDS             complement(1744..>2272)
                     /locus_tag="VFI12_02470"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HET7055295.1"
                     /translation="TLHAVNSGALPHTLTIPDVGDTGEVVAGASFDLNVNLPPGEYPF
                     HCAIPGHEAAGMVGKLIVEAGTAPAASGGVASPAAGGPAAAPAGPVEVDMVDLAFNPT
                     EVTIPANTDVTIHAVNSGALPHTFTIPDVADTGEVAPGSSTDVKVNLPPGEYDFHCAV
                     PGHEAAGMVGKLIVK"
BASE COUNT          372 a          727 c          759 g          414 t
ORIGIN      
        1 agcgcgcctt cgatggcggc tgtaaggagg tggccggcca cgtggacatc tgaaagggcg
       61 tatctcgtgc caagctccgc aacgatcgcc gtgagatcga gcgcctcgag ggtgcggcga
      121 gcagtcagga gcggagtctc cgcggctgcc tggagcgcag cctgcatggc ggcggatcgg
      181 gcctgcttct cgccggcggt ggccttgggt aaggcggccg cggcccggta tcgaccgtag
      241 cagtcctcgt cgtcgcgggc cgatgccgaa agttcgtcga cgagcgcgtc caggcgttta
      301 gcagccggcg cgagggcggt gtcggcctca ggggccctga gcgttagccg ggcgaccatc
      361 gccgccagag acgcacccat cgcaccggcg taggcggccg cgctgccgcc accgggagtg
      421 ggggaaccgg agcgaagggc gctcaggtag tcgtgaatcg tgcgattgtc gggcgcattc
      481 gtttccatgg acccatggta tcgggtccgg cggcgctacg ctcgcccctc gaaccggagc
      541 tggtcaccga cgcggacatc tgctcgcgcc gcggagtccg ggagcatctc gatcacgcca
      601 gcggcgttcg tgaagtccca gcgccacgcc ttcatggcgg ggcggacctt cgtgacacgg
      661 ttgtccttgt cgaaataaat caggtcgatc gggaagcgca tgaagttcgt gtggatgccg
      721 cgtgctggcc ggaagaccat cccatagccg tccgggatcg acttgcggag catgagcccc
      781 ttgaagctgc cccagagccc gccggcctcc tcgatgtcgt cgccgacgat ctggccgttc
      841 cgcacattcc gcacgattga tcgctgatta cccacatacc cgcctttatt gccattcgct
      901 gcccgtttga aacaaacgta tagcggccgg cagtccggcg caatccccgg ggcgggatca
      961 gttatcgctt cgtggtttcg gcggcgatcg cgtccaactc ggccaggaac cgattctcat
     1021 cgtggaactc gatgcggtag ttccgttcgt cggcggttgg ggcgggaggg aggacgaccg
     1081 agatccagcc ttcctcggcg ccccgacaga tcagctcggt tacgcgcgct tcgaaccggt
     1141 tgtagggcat cttcggctgc aggagccggt aatactcctc gccctggccg ttcctgctcc
     1201 agatcaggcg gacgatctgc cggtgcttgg tcacgatcgg ggaagcagcc agctcattgg
     1261 ccatgaggcc gcgcaaaatc gcgttcatct tgcccgtagg ctgcatcggc gagccgcaga
     1321 ccgggcacgc gccatcgatc gcttcctcat gccggaagct gcgccggcaa tgatcacacc
     1381 acaggtacgt cttgcttgaa tcagctcgct gcttgctcac gatcgggacc tcaaaaacga
     1441 acggcccgga gcgaaagtca tccgcccggg ccgtaatgat tcgctgattg cgctactcgc
     1501 ggatgccgtg ctcttcgtag cggttcgcgc cgccaccgaa gccgcggttg accgcgccgg
     1561 ggtgctcgcc acccatcttg tccgcccaga cctggatgcg gtgcgtcagg tagacaccga
     1621 agaggaccat ggcgacggcg aggaacactt cgatggtgtc cgacaggacg ccgtagttgg
     1681 ccaggttgtc aatccggaaa tcaccgatgc tgcgaatgat gtcgcccatg atttgccctg
     1741 tccttacttg acgatcagct tgccgaccat gccggcggct tcatggcccg gcacggcgca
     1801 gtggaagtcg tattcgcccg gcggcaggtt gactttgacg tccgtgctcg atccgggcgc
     1861 cacttcgccg gtatcggcca catccggaat tgtgaaggtg tgcggcaacg caccactgtt
     1921 cacggcgtgg atcgtgacgt cggtattcgc cgggatcgtc acctcagtcg gattgaaggc
     1981 cagatcgacc atgtcgacct caaccgggcc agccggcgcc gcggctgggc ctccagctgc
     2041 tggcgaggcg accccaccgc tggccgccgg cgccgtcccc gcttcgacga tcagtttgcc
     2101 gaccatcccg gccgcttcgt ggccggggat ggcgcagtgg aagggatact ccccgggcgg
     2161 gagattgacg ttcagatcga aactcgctcc cgcgaccact tcaccggtgt cgccgacatc
     2221 cgggatggtg agggtatggg gcagggcgcc gctgttgacg gcgtggaggg tg
//
LOCUS       DASSGX010000138         4052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_896914, whole genome shotgun sequence.
ACCESSION   DASSGX010000138 DASSGX010000000
VERSION     DASSGX010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896914"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VFI12_02475"
     CDS             complement(<1..235)
                     /locus_tag="VFI12_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET7055296.1"
                     /translation="MHTYLVISTAGPNRDLSKGTREQPYWDGHADFIDRLVEVGVIFL
                     GGPLVDEGGAVIAVRAKDEAEVREILADDPWYRY"
     gene            complement(318..707)
                     /locus_tag="VFI12_02480"
     CDS             complement(318..707)
                     /locus_tag="VFI12_02480"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HET7055297.1"
                     /translation="MTWLWIVLAVLTLMLLFVVTVLVWIRRSGMTPAEATNWLREMGI
                     DLVKLPGRLRRVAADPRTPRRARWWLIGLAIYIASPIDPIPDFLPVIGYLDEVVLVPL
                     ILRHIRRMIPDDVWQEHFPPRPEPTAG"
     gene            complement(723..2438)
                     /locus_tag="VFI12_02485"
     CDS             complement(723..2438)
                     /locus_tag="VFI12_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HET7055298.1"
                     /translation="MTLIAPEDRLPGADEPRAPFVSVLPTVRLLAEESSAHPFATTIA
                     AAWSCYGAKPARVENVLKLVHGPGPANVSVEAAADRADRRDRALRLYSDLFAAGHHTT
                     FQHANFTFVLDNVSRLAIWSFFHAHPHYNSEQVSQRYREVSGAVMTTPDLPAPQLVIY
                     RAALEQSLEGYRRLTDILSVDALAGYTQVFPSRVKATGDEAEAKVASAVQKKAQEVAR
                     YVLPLATPAHLYHTVNGLTLLRYLVLSSQPDVPTEVRHIVQRMVEEVLAIDPYFLGTP
                     DYPLNLRPLLEEETLESQLYARWHETVGQRIDDAERFCTDFDTQLGTWRNSRLAAFNP
                     DGEALLANAVRTVLGASDMPDDDALAAVLSGSDNQYLGHALYLGMNSKLMQTLNHVPF
                     TFQKRISGAEDAQNQRHRGTLASRPVLQAHLRRDPDVITPWAIARNPEALAVYQQTVQ
                     GLWDAKNALIDAGAPVETALYLLPNSHRVRFYESGTLMTYYWKWIKRLCYDAQREIFE
                     TAVEEVAQVRAVYPRIGHYVDGPPCVMRSRTQTRPFCPEGERYCGIPVWRNHQFETLE
                     ERRVL"
     gene            complement(2510..3451)
                     /locus_tag="VFI12_02490"
     CDS             complement(2510..3451)
                     /locus_tag="VFI12_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055299.1"
                     /translation="MLAVMQVGFGVRVIRVFAVLGLLLGTLVVGDKAPAIAAVDCAIP
                     TSGVAPVSTPIVATPIASAIAATPVSERDQLKTSLRDLVQVVSACKSAGDFQTMSRLV
                     TEKYLGQVYGGGPRMSRETFLVIAETLPTPKIRFRAFDDLSIIGPGEARANVTLIAGN
                     QLIFERLSFVEEKNRPGQWLIDSTEPLRVRPPRDHANVRITITGNRYSPDELTADNAT
                     VEIRASNSDKEDHELLVLKLDTGLTTHDVLTTPGPNLPDGARYIGQVTIPARESGTLV
                     LVDLKPGTYTIVDLLPAANGVPHLAVGMEATLTVTGE"
     gene            3564..3866
                     /locus_tag="VFI12_02495"
     CDS             3564..3866
                     /locus_tag="VFI12_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055300.1"
                     /translation="MDDSTVQVNPDPFEAIESLAQTVAHLALQLTIAQIQMRALGSVI
                     EEAGLVGGDAVSTAAGRIARMHAGRFLRENLGPELTEMIDTADLERQIVAYLSAGT"
BASE COUNT          721 a         1264 c         1365 g          702 t
ORIGIN      
        1 cgtaccgata ccagggatca tcggcgagaa tctcgcgaac ttcggcttca tccttggcgc
       61 ggacggcgat cacagcccct ccctcatcga ccagcgggcc gccgaggaag atgacgccga
      121 cctcgacgag gcggtcgatg aagtcggcgt ggccgtccca gtagggttgc tcgcgagtgc
      181 ctttcgacaa atcgcgattc ggaccggcgg tcgagatcac gaggtacgtg tgcatttgcc
      241 ctcccgcggg cacccatcgg ggatcatccc gttgacccca gcgtactgag cgacccgggc
      301 aggatctgtc cgcaaaatca accggcggtt ggctcaggtc gaggcgggaa gtgctcctgc
      361 cagacgtcgt ccgggatcat ccggcgaatg tggcggagga tcagcgggac cagcacgact
      421 tcgtcgaggt agccgatgac cgggaggaag tccgggatcg ggtcgatcgg gctcgcgatg
      481 tagatagcca agccgatcag ccaccaccgg gcgcggcgcg gggtgcgcgg atcagccgcg
      541 actcgccgga gccggccggg cagtttgacc aggtcaatgc ccatttcccg gagccagttc
      601 gtcgcctcgg ccggcgtcat tccggatctg cgaatccaga ccagcacggt gacgacgaac
      661 agcagcataa gcgtcaggac ggcaagcacg atccagagcc aggtcatgga ttcagtcgat
      721 cgttagagga cgcggcgctc ttcgagggtt tcgaactggt ggttgcgcca gaccgggatg
      781 ccgcagtagc gctcgccctc ggggcagaaa ggtcgcgtct gggttcgact gcgcatcacg
      841 caaggcgggc cgtcgacgta gtgaccgatc cggggataga ccgcgcggac ctgggcgacc
      901 tcctcgactg ccgtctcgaa gatctcgcgc tgggcgtcgt agcagagccg cttgatccac
      961 ttccagtaat aggtcatcaa cgtgccactc tcgtagaagc ggacgcggtg actgttaggg
     1021 agcaggtaga gggcggtctc gaccggcgcg ccggcatcga tcaacgcgtt cttggcgtcc
     1081 cagagcccct gcaccgtctg ctggtagacc gcgagcgctt ccgggttgcg ggcgatcgcc
     1141 cacggcgtga tcacgtccgg atcgcggcgg agatgcgctt gcaataccgg tcgggaggca
     1201 agcgtgccac ggtgacgctg gttctgggca tcctccgcgc cgctgatccg cttctggaag
     1261 gtaaacggca cgtggttgag cgtctgcatc agcttggagt tcatgccgag gtagagcgcg
     1321 tggccgaggt actgattgtc gctcccgctc agcaccgcgg ccaatgcatc gtcgtccggc
     1381 atgtcggagg cgccgagcac ggtccgcacg gcgttggcca gcaacgcctc gccatccggg
     1441 ttgaaggcgg caagccggct attccgccag gtgccgagct gcgtatcaaa atcggtacag
     1501 aagcgctccg cgtcatcgat tcgctgtccg accgtctcgt gccagcgggc gtacaactgc
     1561 gactcgagcg tctcctcctc gagcagcggc cgcaagttga gcggatagtc cggcgtgcca
     1621 aggaagtatg ggtcgatcgc caggacctcc tcgaccatcc gctggacgat gtggcggacc
     1681 tcggtcggca cgtccggctg actggaaagg acgaggtagc gaagcagcgt caggccattg
     1741 acggtgtgat agaggtgcgc cggcgtggcc agcggcagga cgtaccgcgc gacttcctgc
     1801 gccttcttct ggacagcgga agcgactttc gcttctgcct cgtcgccggt cgccttgacg
     1861 cgtgacggga agacctgcgt gtacccggcc agcgcatcga ccgagaggat gtcggtcagc
     1921 cggcggtatc cctccaggct ctgctcaagc gcggcccggt agatcacgag ctgtggcgcc
     1981 ggcagatcgg gtgtcgtcat gaccgcgccg ctgacttcgc ggtatcgctg cgaaacttgc
     2041 tccgagttgt aatgcgggtg cgcgtgaaaa aaggaccaga tcgccagccg gctgacattg
     2101 tcgagcacga aggtgaaatt ggcgtgctgg aaggtggtgt ggtgtcccgc cgcgaagaga
     2161 tccgaataga gccgcaatgc ccgatcccgg cggtcggctc gatctgccgc ggcctccact
     2221 gacacgttcg ccggaccggg accgtgcacg agcttgagca cgttctcgac cctagccggt
     2281 ttcgccccat agcaggacca ggccgctgcg atcgtcgtcg caaacgggtg cgcggacgat
     2341 tcctcggcca gcaggcgcac cgtcggcaac accgagacga agggcgcgcg cggctcgtcg
     2401 gcacccggca aacggtcttc aggggcaatc aacgtcatcg agcagcggtc tccaaacggc
     2461 agaaccggag acaggccaac cggtcgcgcc tagcatacca atcggaggcc tactcgccgg
     2521 tgacggtgag ggtggcctcc atgccgaccg ccaggtgcgg cacgccattg gcggccggaa
     2581 gaaggtcgac gatcgtgtac gtcccgggct tcaggtcaac caggacgagc gtgccactct
     2641 cccgcgccgg gatcgtgacc tgcccgatat accgggcgcc gtccggcaag ttcggaccgg
     2701 gcgtggtcag gacatcgtgt gtggtcaggc ccgtgtccag cttgagtacg agcagctcgt
     2761 ggtcctcctt atcggagttg ctggcgcgga tctcgacggt cgcgttgtcg gcggtcagct
     2821 cgtcgggact gtaccggttg ccggtgatcg tgatccggac attggcgtgg tcccgcgggg
     2881 gccggacgcg caatggttcc gtggagtcga tcagccattg ccccggacgg ttcttttctt
     2941 caacgaagct caaccgctcg aagatcaact ggttgcccgc gatcagggtg acgtttgcac
     3001 gggcttcgcc agggccaatg atcgagaggt cgtcgaaggc ccggaagcgg atcttcgggg
     3061 tcggcagcgt ctcggcgatc accaggaacg tctcgcgcga catgcgcggc ccgccgccgt
     3121 acacctggcc gaggtacttc tcggtcacga ggcgggacat cgtctggaaa tccccggcgg
     3181 atttgcaggc ggagacaacc tggacgagat cgcgcaggct ggtcttgagc tggtcgcgct
     3241 cggaaaccgg tgtcgccgcg attgcgcttg cgatcggcgt tgcgacgatg ggcgtcgaaa
     3301 ccggggcaac gccggaggtg ggaatcgcgc agtcgacagc ggcgatcgcc ggcgccttat
     3361 ctcccacgac gagcgtgccg agcaggagac ccagcacggc gaagacgcga atcacccgga
     3421 caccaaatcc cacctgcatg accgccaaca cccacgctcc atacaaacca gatacccaat
     3481 gtatgatcgc ggggatcggc gaattggatt caccgggatg cgggacacct agtctagcag
     3541 gggcggcacc acgcaggcag gatatggacg attcaactgt gcaggtcaac ccggatccgt
     3601 tcgaggcgat cgagtcattg gcccagacgg tcgcgcatct ggccttgcaa ctgacgatcg
     3661 cgcagatcca gatgcgcgcc ctgggctcgg tgatcgagga ggcggggctg gtcggtggcg
     3721 acgcggtttc gacggctgcc gggcggattg cccggatgca tgccggccgc ttcctgcgcg
     3781 agaacctcgg accggagttg accgagatga tcgacaccgc cgacctcgag cggcagatcg
     3841 tcgcctacct gagcgccggc acgtaacgag tcctgtttcg catcgacctt tgtacttcgc
     3901 atcccgctat tcggcctagc caacttatac ccgtgatatg gtgcaggggc gacacgtttt
     3961 tcgttcggcg atgccgccgg ttgttccacc cgttcccgta ccatcggagt tttcccaatg
     4021 tggcttgtca ccggcggcgc cggattcatc gg
//
LOCUS       DASSGX010000139         4084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_243762, whole genome shotgun sequence.
ACCESSION   DASSGX010000139 DASSGX010000000
VERSION     DASSGX010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243762"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /gene="ligD"
                     /locus_tag="VFI12_02500"
     CDS             complement(<1..872)
                     /gene="ligD"
                     /locus_tag="VFI12_02500"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HET7055301.1"
                     /translation="MPAEKTTWEIAGRAVQVSSLDTVYWPEDGLTKGDLLAYYREIAP
                     VMLRYLQDRPVTLRVFPKGISGSGHYRRDRPPQAPDWLRGADYETATNRHTLRTLIID
                     DAAGLVWCANTGAIEFHIWSARLPDLAEPDLAIFDLDPGEECIFRDVLRAAMVLREEL
                     ERLGLQGYPKTSGGRGLHVLVPLAPGHSFEAVRDWVSGCADELAATHPARFSSAGGAT
                     HRGDRITIDYAQNSIGRNTAAPYTVRGLPHAPVSTPVTWDEVEAGEIDPNDFTLRTVP
                     ERIQRLGDLLAPML"
     gene            complement(879..1307)
                     /locus_tag="VFI12_02505"
     CDS             complement(879..1307)
                     /locus_tag="VFI12_02505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03667 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HET7055302.1"
                     /translation="MGFEIDTSTEFGHNVVKRLDEQKLAWLTTVDSNGTPQPNPVWFI
                     WDSGVIYIFSRPNQAKLANIRRSSRIAVNLEATTDEEHVTIFTGEAEIIDRADLPGDI
                     LDRYAERYAEGMKGIKMTRAEYEAAYTIPIRFTPKKLRGW"
     gene            complement(1352..2698)
                     /locus_tag="VFI12_02510"
     CDS             complement(1352..2698)
                     /locus_tag="VFI12_02510"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HET7055303.1"
                     /translation="MNQERAADDLERTARQIPYSEPMAGRPDEASPSRFRRLFNVLGP
                     GFITGASDDDPSGIGTYASVGASLGFAPLWTALASFPLMATIQYTCAKVGMVSGTGLG
                     GVLRQHYPRWVLYSSVTILIVANTINAGADIGAIAAGIHLLVPVPVAATIIPVTIAIL
                     ALQIFGSYRLIARVFKWLTLALFGYIGAAVFARPDVHAVLRGTFTPTLRFDQAYIMAM
                     VALLGTTISPYLFFWQASEEVEEEISEGRTSQLLRQGATRSELRRAGWDVGIGMLFSN
                     VVMYFIILTTAATLHQEGLTNVQTATQAAQALRPLAGRGAEALLALGLIGSGFLAVPI
                     LTGSGAYAAAEAWGWRYGLDEKPKQAKPFYAVIIVATLVGMMINFIGINVIQALYWTA
                     ILNGLLAPPLLVLITVVANNAAVMGEQRVSRPIHLACWVAAAVMTLAALALIATWL"
     gene            complement(2722..3165)
                     /locus_tag="VFI12_02515"
     CDS             complement(2722..3165)
                     /locus_tag="VFI12_02515"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HET7055304.1"
                     /translation="MDRQDSRPLWLTLMNRVVNPVVRLILRSPLHPLLSRYLLLLTVT
                     GRATGRSWTLPLQYAERDGAAWIVPGNPAHKRWWRNLNDDASATLTIRGKSYIGIGEA
                     LTGAAADAARPGLAGSPLEGLARRSGNSVIVRVHDLRPIGSIERR"
     gene            3394..4008
                     /locus_tag="VFI12_02520"
     CDS             3394..4008
                     /locus_tag="VFI12_02520"
                     /inference="COORDINATES: protein motif:HMM:NF021384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HET7055305.1"
                     /translation="MADTRHGHEMVGQARSRDDVSTRTIIALDRAIYRFAGRWLFGFN
                     LIVGGYAALVILAPVLSALGYGGAARPIYRYFGLFCHQDPDRSFHLAGHQFACCERCA
                     AIYGSMALFGLLFAFARGRVRNPRYPDLVLLISPVVLDGMAVGSGLYGGNAVMRVITG
                     TLFGVAVIWLVYPRFESGFAAMRTRLETLFDRLAAQGRAKPLAP"
BASE COUNT          669 a         1304 c         1396 g          715 t
ORIGIN      
        1 tcgagcatcg gggcgaggag gtcgccgagg cgttggattc gttcggggac ggtgcgcagc
       61 gtgaaatcgt tcgggtcgat ctcgccggct tccacctcgt cccacgtcac cggtgtcgag
      121 acgggagcgt ggggcagtcc ccggacggta tagggcgcgg ccgtgttgcg gccgatgctg
      181 ttctgggcgt aatcgatggt gatccggtcg ccccgatgcg tagcgccgcc ggcggacgag
      241 aaacgcgcgg gatgggtggc ggcgagctcg tcggcacacc cactgaccca gtcgcggacc
      301 gcttcgaacg aatggccggg ggcgaggggg accagaacgt ggaggccgcg gcccccgctg
      361 gtcttgggat atccctgcaa ccccaaccgc tccagctcct cgcgcagcac catcgcggcc
      421 cgcaatacgt cgcggaagat acactcctcg ccgggatcga ggtcgaagat ggccaggtcg
      481 ggctcggcga gatcgggaag gcgcgcgctc cagatgtgga actcgatcgc gccggtgttt
      541 gcgcaccaga ccaggccagc cgcgtcatcg atgatcaggg tccggagggt gtgccgattc
      601 gttgccgtct cataatcggc cccgcgcagc caatcgggcg cctgcggcgg gcgatcccgg
      661 cggtaatgac cggagccact gatgcctttg gggaagacac ggagcgtgac cggccgatcc
      721 tggagatacc gcagcatcac cggcgcaatc tcccggtagt aggccaggag gtctcccttg
      781 gtcaggccat cctccggcca gtagacggta tccaggctcg acacctgcac cgctcgcccg
      841 gcaatctccc aggtcgtctt ttcagcgggc atcgcgcctt accagccgcg aagtttcttg
      901 ggcgtgaacc ggatcgggat ggtgtaggcg gcttcgtact cggcgcgggt catcttgatg
      961 cccttcatgc cctcggcgta tcgctccgca tagcgatcga ggatgtcgcc cggaaggtcg
     1021 gcacggtcga tgatttcggc ctcgccggtg aagatcgtga cgtgctcctc gtcagtcgtg
     1081 gcctcgagat tgactgcgat gcgcgaggaa cgccggatgt tggccaactt ggcctggttc
     1141 ggccgactga agatgtagat cacgccgctg tcccagatga accagaccgg attcggctgg
     1201 ggcgtgccgt tgctgtccac cgttgtcagc caggcgagct tttgctcatc gagccgcttc
     1261 accacgttgt ggccgaattc cgtcgacgtg tcgatctcga atcccattgc cgtctcccct
     1321 tcctgatggc tcgtccggcg gtcattatcc actagagcca ggtggcaatc agggcgaggg
     1381 ctgccagcgt catcacagcg gcggcgaccc agcaggcgag gtggattggt cggctgaccc
     1441 gctgctcacc catcaccgcc gcattgttgg cgacgacggt aataaggacc agcagcggcg
     1501 gggcgagcag gccgttgagg atggcggtcc agtagagcgc ctggatcacg ttgataccga
     1561 tgaagttgat catcatgccg accagggtcg ccacgatgat cacggcgtag aagggctttg
     1621 cctgcttcgg cttctcgtcg aggccatagc gccagcccca cgcttccgcg gcggcatagg
     1681 cgcccgatcc ggtcaggatc ggaaccgcca ggaagccaga cccgatcagg ccgagcgcaa
     1741 gcagagcctc ggcgcctcgc ccggcgagcg gccgcaacgc ctgggcggct tgggttgcgg
     1801 tctggacatt ggtcagcccc tcctggtgga gggtggctgc cgtagtcagg atgatgaagt
     1861 acatcacgac attcgagaag agcatgccaa tgccgacatc ccagccggca cggcgcagtt
     1921 cgctgcgcgt ggcgccctgg cgcagcaact ggctggttcg gccctcgctg atctcctctt
     1981 cgacctcctc actggcctgc cagaagaaga gatagggcga gatcgtcgtg ccgagcaggg
     2041 cgaccatggc catgatgtag gcctggtcga agcggagcgt cggggtgaaa gtgccgcgca
     2101 ggacggcgtg cacgtccggg cgggcgaaga cggccgcgcc gatgtacccg aacagggcca
     2161 gggtcagcca tttgaacacc cgcgcgatca gccggtacga gccgaagatt tgcagcgcga
     2221 ggatggcgat cgtgaccggg atgatcgtcg ccgccaccgg aaccggaacg agcaggtgga
     2281 tgccggccgc gatcgctccg atgtccgcgc cggcgttgat cgtgttggcc acgatcagga
     2341 tggtgacgga cgagtagagc acccagcgcg ggtagtgctg gcggagcacg ccgccgagcc
     2401 cggttccgga caccatcccg accttggcgc aggtgtactg gatggtcgcc atgagcggga
     2461 atgaggcgag cgcggtccag aggggagcga agccgaggga ggcgccgacg ctggcatagg
     2521 tgccgatgcc ggaggggtca tcgtcggacg cgccggtgat gaagccgggg ccaagaacgt
     2581 tgaagaggcg tcgaaatcgg gagggcgacg cctcgtccgg tcgcccggcc atcggttccg
     2641 agtaggggat ctgccgcgcg gttcgctcga gatcgtccgc cgctcgctcc tgattcatcg
     2701 aagcgcacct gcttatcggc gtcagcgtcg ctcaatcgat ccgatcgggc ggagatcatg
     2761 aacccgcacg atcaccgaat tgcctgatct gcgcgccagc ccttccagcg gcgatcccgc
     2821 caatcccggc cgcgcggcat ccgcggcggc gccagtcagc gcttcgccta tcccgatata
     2881 cgactttccc ctgatcgtga gcgtagcgga ggcgtcatcg ttcagattgc gccaccagcg
     2941 cttgtgggcc gggttgcccg ggacgatcca ggccgccccg tcgcgttccg cgtattggag
     3001 cgggagtgtc caactccgcc cggttgcccg cccggtgacg gtcaacagca ggaggtagcg
     3061 actcagcaac gggtggagcg gcgagcgcag gatgagccgg acaaccgggt tcacgacccg
     3121 gttcatgagc gtgagccaaa gcggcctgga gtcctggcgg tccatcgcga atcacctccg
     3181 gagcatgccg gatcgtccga ttccggtcgc acgatatcgc gggggatgtt gcgcccgcgt
     3241 gcggcgtttc cccgcttccg ttgcaaattc gcctcacccg gcgtcgaagg tggcgggatt
     3301 cgcgaccgat gggccatacc ggggaagaga gcgtgccgcc taccctttca tcggtacccc
     3361 gcgtcggtga ggcagatttt cggcatgcag caaatggcag atacccggca cggacacgag
     3421 atggttgggc aggcgcggag ccgggatgac gtgtccacgc ggacgatcat cgcgctcgac
     3481 cgggcgatct accggttcgc cggtcgctgg ctcttcggct ttaacctgat cgttggcggc
     3541 tatgcggcgc tcgtcatcct ggcgccagtc ctgtccgcgc tcgggtatgg cggcgcggcg
     3601 cggccaatct atcgctactt cggtctcttc tgccaccagg atccggaccg cagctttcac
     3661 ctggcggggc atcaattcgc ctgctgcgag cgatgcgcgg cgatctacgg ctcgatggcg
     3721 ctcttcggcc tgctcttcgc attcgcccgg ggcagggtcc gcaacccccg gtatcccgat
     3781 ctggtgctgc tcatctcgcc ggtcgtgctc gatggcatgg ccgtcgggtc cgggctgtat
     3841 ggcgggaacg ccgtgatgcg ggtgatcacg ggcacgctct tcggagtagc cgtgatctgg
     3901 cttgtctatc cgcgcttcga gtccggcttc gccgcgatgc ggacgcgcct ggaaaccctc
     3961 ttcgaccgcc tcgccgccca aggccgcgca aaaccgctgg caccgtgatc cgcctgtaag
     4021 ggtttgctgc cgcaaacccg cccgttcccc gaatgcccat tgcaatgcac ccgccgatgt
     4081 aggg
//
LOCUS       DASSGX010000140         7197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_570123, whole genome shotgun sequence.
ACCESSION   DASSGX010000140 DASSGX010000000
VERSION     DASSGX010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7197
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570123"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..1873
                     /locus_tag="VFI12_02525"
     CDS             248..1873
                     /locus_tag="VFI12_02525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HET7055306.1"
                     /translation="MIQGCVWIGHVPWRVRRAASRYAVVIALTVSGCSTDWLPHVDLA
                     PKYEPPEYVVPVSWKGSSPFVEATPSDGELRSDWWALFNDPVLNKLEEQAMAANPDLQ
                     AAAERFVQARDVMMQVRSQRIPHIGLGGKATDSHLHVNPSVYDPDTPLTGSVGAGGGI
                     ASWEPDFWSAIRNATRVEIYRAQGRAADWGLARLSLQAEIAADYFTLRGFDAQAAIYK
                     QSIDLYRKSLDLVKAQFAGAIASALDVARVESLLFSTETKYAQIQGQRQVAEQAIAVL
                     VNMVPANFKIDAVDDLRVAKFTVPPSIPSTLLERRPDIAAMERRMAEANRAIGIARAA
                     FFPDVRFRADGGILDVGFNAVEIAKFVTGFWSYGSLVEVPAFGGGYRRAQLQRTWSAY
                     REMEDRYRSTVLNAFREVENNLSLTNRLTVAAERQDAAVGATFKAQNLTTELYKGGLA
                     SSLELIYAQVATLTARIDAVQIKAELLRSSVALNRALGGGWDRKQLPTDDQIQPFGTL
                     QYTNLKKPPPAGGIDVNADNNSIHNDLTKPAVR"
     gene            2509..5679
                     /locus_tag="VFI12_02530"
     CDS             2509..5679
                     /locus_tag="VFI12_02530"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7055307.1"
                     /translation="MNQVVLIALRRPYTFVVMTIVIVLFGGLTVSHMPTDVFPNITIP
                     VTSVVWVYSGLLPQQVEGRITYLFERFLTSTVEGIKYIHSHSYYGSSITNIFLQDGVD
                     VGRAEADITAIAQNVVKALPPDISPPMIMRLAPSSIPVAMLEISSDTMTPAELFNLAY
                     MRIRPLLVTVNGAILPQPYGGQPMQVMINLDQQKLLARHLTPADIHEALMRQYLILPS
                     GDIKIKSTDWIVRTNASPMRIEDFANIPVKREGNAFIYLRDVATAQLMGRVQQNAVLV
                     KGKQTVIIVVMKSTEASTLAVVDGIKQMIPRAEKLVPEGVKIRLLDDASTFVKDAISD
                     VLHEMLTAGALVGLIVLLLLGSWRPTVIVWTSIPLSILTAIIGLHALGETINVMTLSG
                     LALAVGILVDDATVMIENIDRHLEMGKVLEEAIIDAANQIVVPTFVATLCIAIVWLPL
                     FELGGVAGFLFKPMAEAVIIAMLASFILSRTLVPTMAKYMLSAHHDDQARAVASQNVF
                     VRFQQGFEHRFNQFREGYGLLLEQMIAHRRAFVTISLAIALASLSLFFFLGRDYFPEI
                     RSGVIQMHMRAPLGTRIEVSGRIATLVANSIEELLPGEVENIVSNCGLPIGPHNLAFI
                     PTPTIGSQDCDLTILLKDEKSPVWDYRRILRAGLTERYPGTEFTFQPSDLTAKILNFG
                     APAPIDVQTNGPDIYPNYEFARKLADKLRLIPGATDVVIQQTMRTPTLMVEGNRTFGL
                     GVNRTEKDIASNLLMTTAGSQQVDQIYWLDPKTGMSYPINVYTPQPQINSVNSLKTIP
                     VESSSNGSNGHTDHDVQLLGNLATLSAEGTPGVVTHANIMPLFDIYVSTEGRDLGGVL
                     ADVEHVAETMKADLPRSAAIEIHGQAALMHDAYFELIFGLLAAIGLVYLLIVVNFQSW
                     LDPFIIITALPGALAGIAWALFLSHTRLSEPALTGAIMTMGTGTANAILVVSYARERL
                     QEHGDAVRAAIEAGKTRLRPVLMTASAMIIGMVPMSVGYSQNAPLGRAVIGGLLVATV
                     FTLFFVPCVYAMIYGRRPAQQKGPAS"
     gene            5730..7010
                     /locus_tag="VFI12_02535"
     CDS             5730..7010
                     /locus_tag="VFI12_02535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET7055308.1"
                     /translation="MLYVGHRMYESSGDAALLREKTLEEAVRTVAVVSPKPGALTETI
                     TLPGNIVGWYEAPMYARVTGYVKMWYKDYGDRVKKGDILAEINAPDLDAEYAQAKADL
                     EMQRARYRLAEVTAKRWVALRPNHAVSEQSITVKEQELKAEAAMVKAAEQKIRNIDAF
                     IRFKTIVAPFDGVVTQRNINVGDLVSKEGNLSTPNAISNLFTVADVHMLRVFVNVPAS
                     FGSFLQPDLTADVTVPQVPNRHFTAKFLTVAKGFDVSTRTAVTVFTIDNEDRALWPGS
                     YAQVHLTAPVDRQVFTIPSTALVFQEHGTQVAVVTEDDQVHLKPITVSKLMDNAVEVA
                     EGISTSDRVINNPSAALLEGDKVRVVTPASGYDLVNAPAATDASEPAPVSQPPHASPA
                     SPAPQAPQPAQSPEPQAPPAPLRKPPQALQPIQP"
     gene            7007..>7197
                     /locus_tag="VFI12_02540"
     CDS             7007..>7197
                     /locus_tag="VFI12_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055309.1"
                     /translation="MIQGLVWIGHASWRVRRAASRYAVVIALTVSGCSTDWLPHVDLA
                     PKYEPPEYVVPVSWKGSSP"
BASE COUNT         1424 a         2321 c         2091 g         1361 t
ORIGIN      
        1 tgaacaatcc gaacgccgcg ttgctcgaag gcgataaggt ccgcgtcgtc acgccggcgt
       61 caggctacga tctcgtcaat gcccccgcag cgacggacgc gtctgagccg gcacaggtat
      121 ctcagccgtc acacgcatcg ccggcgccgc cactaccaca agccccccag ccggcacagt
      181 caccagagct tcaggaaccg ccagcaccgc tccggaagcc gccacaggcc ctgcagccga
      241 ttcagccatg attcaggggt gcgtgtggat cggacatgtg ccgtggagag ttcgccgcgc
      301 tgcgtcgcgc tacgccgtcg tcatcgcgct aactgtatcc gggtgcagca ctgactggct
      361 accgcacgtc gatctggcac ccaagtatga accgccggaa tatgtcgtcc ccgtctcctg
      421 gaaagggtcg agtccgttcg tggaggcgac gccatcggat ggtgagctgc gttcggactg
      481 gtgggcgctg ttcaacgatc cggttctgaa caagcttgaa gaacaagcca tggcggccaa
      541 tccggatcta caggccgctg ccgagcggtt cgtccaggcc cgcgacgtca tgatgcaggt
      601 ccgctcacag cgaatcccgc atatcggttt gggcgggaaa gccacggaca gtcacctaca
      661 cgtcaatccc tccgtatacg accctgatac tccgttgacc ggatcggtgg gagccggtgg
      721 cgggatcgca tcctgggagc cggacttctg gtccgcgatc cgcaatgcga cgcgcgtcga
      781 gatctaccgc gcccagggac gcgccgccga ctggggactc gcccgcctca gcctgcaggc
      841 cgaaattgcg gcggactatt tcacactgcg tggcttcgac gcgcaggccg cgatctataa
      901 gcaatcgatc gacctctaca gaaagtcact cgacctcgtg aaggcccagt tcgccggcgc
      961 cattgcgtcg gcgctcgacg tcgcccgcgt cgaatccctg ttgttcagta cggagacgaa
     1021 gtacgcgcag atccaaggcc aacgtcaagt ggccgagcaa gcgatcgcgg tcctcgtcaa
     1081 catggtgccg gccaacttca agatcgacgc ggtggacgac ctgcgtgtgg caaagttcac
     1141 ggttccgccg agcattccgt ccacgttgct ggaacggcgg cccgacatcg ccgcaatgga
     1201 acgccggatg gcggaggcca accgcgccat cggcatcgcg cgggccgcct tcttccccga
     1261 cgtgcggttc agagccgatg gaggcatcct cgacgtcggt ttcaacgccg tcgaaatcgc
     1321 caagttcgtc accgggttct ggtcctatgg ctccttggtg gaggtgccgg ccttcggggg
     1381 cgggtatcga cgcgcccaat tgcagcggac ctggtctgcc tatcgagaga tggaagaccg
     1441 ctatcgttcg accgtgctca acgcctttcg agaagtcgag aacaatttaa gtctgacgaa
     1501 ccggctgacc gtcgcggccg aacggcagga cgccgccgtc ggcgcgacgt tcaaagcgca
     1561 aaatctgacc acggagctgt ataagggcgg attggcgtcc agcctcgagc tgatttacgc
     1621 gcaggtggcc actctcacgg cgcgcatcga cgcggtacag atcaaggccg aactactcag
     1681 atcgtcggtc gcactcaacc gcgcgctcgg cggcggctgg gatcgcaaac aactgcctac
     1741 cgacgaccag attcagccct tcgggacgtt gcaatatacc aatcttaaga aaccgccgcc
     1801 cgccggtggc atcgatgtca acgcggacaa taactcgatc cataacgatc tgaccaaacc
     1861 cgccgttcgg taaccgccgc tcgaaagctc taggtcaacg gtctgctgcc cccttcacta
     1921 tgctgctctg cgagccagtc gtgcacgggt tcgcagagac tacggcaagc gccttgaggg
     1981 tacgatggtt actcaatccc tgtctatcag gttttcttac aggcggaggg caatcccacg
     2041 gatagtgcac cgaatcttcg cctccttccg acgaccttga agtgacggcg aacgtggccg
     2101 atacgaaccg gcgactgatt ggactcacac aacgatctgg ccgattccgc acagcacgat
     2161 ctgtgcgccc gccaacacgc gtgctcttgc acacgtgaac gtgccaccat cgacacgcgc
     2221 gtaatccgtc ctaaacaacg gtggtcctgg ctcaagctct gcactatgac ggcccggacc
     2281 atgtagaacc cacattaatc gactcaaaac ggccaacaca tcttccggta tccggtttgc
     2341 ttcatatccc ggtagacacg gtgacgcctg cagcatgacc cggcagatat ttgagcggcc
     2401 gatcatgtcg gcgagaacta tgtcttcccg aagagctgaa tggacgacaa tcttacaccg
     2461 cggtgccgat atgactgatc atccaacctc gtccctcgga cattggacat gaaccaagtc
     2521 gttctcattg ccctgcgaag gccgtacacc ttcgttgtca tgaccatcgt catcgtgctg
     2581 ttcggtggcc tgacggtgtc ccacatgcca accgacgtct tcccgaatat cacgattccc
     2641 gtcacctccg tggtctgggt ctactccggc ctgctgccgc aacaggtgga agggcgcatc
     2701 acgtatttat tcgaacgctt tttgacctcg acggtggaag gcatcaagta catccatagt
     2761 cactcctact atggcagcag catcaccaat atttttctcc aggacggggt ggatgtgggc
     2821 agggccgaag cggatatcac ggccattgcc cagaacgtcg tgaaggcgct gccgccggat
     2881 atttcccctc ccatgatcat gcgtctcgcg ccatcctcca tcccggtggc catgctcgaa
     2941 atcagctccg acacgatgac gcccgctgag ctcttcaacc ttgcctacat gcgaatccgg
     3001 ccgctgctcg tgaccgtcaa cggggcgatc ctgccacagc catacggcgg tcagccaatg
     3061 caggtcatga tcaacctcga tcagcagaaa ttgctcgccc gtcatctgac tccggcggac
     3121 attcacgaag cgctcatgcg gcaatatctc atcctgccgt cgggcgacat taagatcaag
     3181 tcgaccgact ggattgttcg aacgaatgcg tcgccgatgc ggattgaaga tttcgcgaat
     3241 atcccggtca agcgggaagg caacgcgttc atctacctgc gcgacgtcgc caccgcgcag
     3301 ctcatgggcc gcgtgcaaca gaacgcggtc ctggtgaagg gcaaacagac cgtcatcatc
     3361 gtcgtgatga agagcaccga ggcctcaacc ctggcggtgg tggacgggat caagcagatg
     3421 atcccacggg ctgagaaact cgtcccggag ggcgtgaaga tcaggctgct cgacgacgcc
     3481 tcgacgttcg tgaaggatgc gatctcggac gttctgcacg aaatgctcac cgccggtgcg
     3541 ctggtcggcc tcatcgtgct gttgctgctc ggctcatgga ggccgaccgt catcgtgtgg
     3601 acctcgatcc cgctgtccat cctgacggcc atcatcggcc tgcatgcgct cggcgagacg
     3661 atcaatgtca tgacgttaag cgggctggcg ctggccgtcg gcattctggt cgatgacgcc
     3721 accgtgatga tcgagaatat cgatcgccat ctcgaaatgg gcaaggtgct cgaagaggcg
     3781 atcatcgacg ccgccaatca gatcgtcgtc ccgacgttcg tcgccaccct gtgcatcgcg
     3841 atcgtttggc tcccgctctt cgagctgggc ggcgtcgccg gcttcctgtt caagcccatg
     3901 gcggaggcgg tgatcatcgc aatgctggcc tccttcatcc tgtcgcgaac cctggttccc
     3961 accatggcga agtacatgct gtcggcgcat cacgatgacc aggcccgagc ggttgcgtcg
     4021 caaaacgtct tcgtccgctt ccagcagggc ttcgagcacc gcttcaatca gtttcgcgag
     4081 ggctacgggc tgctgctcga acagatgatc gcccatcgcc gcgccttcgt cacgatctcg
     4141 ctggcgatcg cactggcatc cctgtctctc tttttcttcc tcgggcgcga ttacttcccc
     4201 gagatccggt cgggggtcat tcagatgcat atgcgggcgc cgctcgggac gcgcatcgag
     4261 gtgtcaggcc gcattgccac gctcgtcgcc aacagcatcg aggagttgct gcccggggaa
     4321 gtcgaaaaca tcgtcagtaa ttgcggcctc cccatcggac cccataacct cgctttcatt
     4381 ccgacgccga cgatcggctc gcaagactgt gatctgacga tcctcttgaa ggatgaaaag
     4441 tcgccggtgt gggactaccg acgcattctc cgcgcgggtt tgacggagcg ttatccgggc
     4501 acggaattca cgttccagcc gtccgatctg accgccaaaa ttctcaattt cggcgccccg
     4561 gcgcccatcg acgtgcagac caacggcccg gacatctacc ccaactatga gttcgcgcgt
     4621 aaattggcgg acaagttgcg cctgatcccc ggcgcgaccg acgtggtgat ccagcagacc
     4681 atgcgcacac cgaccctcat ggtcgaaggc aaccgcacgt tcggactcgg cgttaaccgg
     4741 accgaaaagg acatcgcctc taatctgctc atgacgaccg ccggaagcca gcaggtcgac
     4801 caaatttatt ggctcgatcc caagaccggc atgtcgtacc caatcaatgt ctatacgccg
     4861 cagccgcaga tcaatagcgt caatagtctg aagaccatcc cggtcgagtc ctcgagcaat
     4921 ggctcaaacg gtcataccga tcatgacgtg cagttgctcg gtaatttggc caccctgtcc
     4981 gcggagggca cgccgggcgt ggtcacgcac gcgaacatta tgccgctgtt cgacatctat
     5041 gtctccaccg aaggacgcga cctcggcggc gtgctcgcgg acgttgaaca cgtggccgag
     5101 actatgaagg ccgacttgcc gcgcagcgcc gcaatcgaaa tccatggcca ggcggccctg
     5161 atgcacgacg cctatttcga gctgatcttc ggcctgctcg ccgcgatcgg gctggtctac
     5221 ctgctgattg tcgtcaactt ccaatcctgg ctcgaccctt tcatcatcat tacggccttg
     5281 cccggcgcgc tggccggcat cgcctgggcg ttgttcctca gccatacgcg gctgtcggag
     5341 ccggcactga cgggggcgat catgacgatg ggtaccggga ccgccaatgc gattctcgtc
     5401 gtatcctatg cgcgcgagcg gctccaagag catggggatg cggtgcgggc ggcgatcgaa
     5461 gccggcaaga ctcgcctccg gccggtgctc atgacggcct cggccatgat catcggcatg
     5521 gtgccgatgt cggtcggcta ctcgcagaat gcgccgctcg gtcgcgccgt catcggcggc
     5581 ttgctcgtgg ccaccgtctt caccctgttt ttcgtgccct gcgtgtacgc aatgatctat
     5641 ggtcggcgac cggctcaaca aaaaggacca gcctcatgat gaacagactc aatggcaaac
     5701 gtcttgcctt tgtcgcaatc gtcttgctcg tgctctatgt cggccatcgg atgtatgaga
     5761 gcagcggcga cgccgcgctg ctgcgcgaga agacgctcga agaggcagtg cgcaccgtag
     5821 cggtcgtctc tcccaagccg ggagcgctga ccgagaccat tacgctaccc ggcaatattg
     5881 tgggctggta tgaggcgccg atgtacgccc gcgtcacggg ctacgtgaag atgtggtaca
     5941 aggactacgg cgaccgggtc aagaaagggg acatcctcgc cgaaatcaac gcgccggatc
     6001 tcgacgccga atatgcgcag gccaaggcgg atctggaaat gcagcgcgcc cggtatcggc
     6061 tcgccgaggt gaccgcgaag cgctgggtgg cgctccgccc caatcatgcc gtctccgagc
     6121 agtcgatcac ggtgaaagaa caggagttaa aagccgaagc ggcgatggtc aaagccgcgg
     6181 aacaaaagat ccgcaacatc gacgcgttca ttcgcttcaa aacgatcgtc gcgccctttg
     6241 acggcgtcgt gacccagcgc aacatcaacg tcggcgactt agtcagtaag gaaggcaatc
     6301 tgagcacccc gaatgcgatc agcaatctct ttacggtggc cgacgtccat atgctgcgcg
     6361 tctttgtcaa tgtgccggcg tcgttcgggt ccttcctgca gccggacctg acggccgatg
     6421 tgacggtgcc gcaagtgccc aatcgtcatt tcaccgcgaa attcctgacg gtggcgaaag
     6481 gcttcgacgt gagcacgcgg accgcggtca ccgtctttac gatcgacaat gaggaccgcg
     6541 cgctttggcc cggctcctac gcccaggtcc atctcacggc accagtcgat cggcaagtct
     6601 tcacaattcc gtccaccgcg ttggtgttcc aggaacacgg cacgcaggtg gccgtcgtga
     6661 cggaggacga ccaagtgcac ttgaaaccca tcaccgtaag caaacttatg gacaatgccg
     6721 tcgaagtggc cgaggggatt tccacgagcg accgcgtcat caacaacccg agcgccgcgt
     6781 tgctcgaagg cgacaaggtc cgcgtcgtca cgccggcgtc agggtacgat ctcgtcaatg
     6841 cccccgcggc gacggacgcg tctgagccgg caccggtatc ccagccgcca cacgcctcgc
     6901 cggcgtcgcc agcgccacaa gcgccccagc cggcgcagtc accggagcct caggcgccgc
     6961 cggcgccgct ccggaagccg ccacaggccc tgcagccgat tcagccatga ttcaggggct
     7021 cgtgtggatc ggacatgcgt cgtggagagt tcgccgcgct gcgtcgcgct acgccgtcgt
     7081 catcgcgcta accgtatccg ggtgcagcac tgactggctg ccgcacgtcg atctggcacc
     7141 caagtatgaa ccgccggaat atgtcgtccc cgtctcctgg aaagggtcga gtccgtt
//
LOCUS       DASSGX010000141         2545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_352931, whole genome shotgun sequence.
ACCESSION   DASSGX010000141 DASSGX010000000
VERSION     DASSGX010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352931"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="VFI12_02545"
     CDS             <1..744
                     /locus_tag="VFI12_02545"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET7055310.1"
                     /translation="LHAELARRGHQPLTDRATAVHARQIQAIANAAADAMAPIERGEL
                     PLPGKSVAHMINEVFGDKTILVKENGGQDLWVYYWPYYQVCEQNCCVAPAEQTAMGYG
                     VIGSMAVKLGRPDHYVVSTSGDGAFQMSIHELGTAAQIKTPVTRVVLDDGAFGWVQWI
                     QRRDLDDRIVATDFEPHVDLVAVAKASNCEAERLTDAGHLRAALERAKAANDAGKPYV
                     LVVPIDQSHHHAEFDRFHDFEPAPGSATA"
     gene            complement(730..1380)
                     /locus_tag="VFI12_02550"
     CDS             complement(730..1380)
                     /locus_tag="VFI12_02550"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HET7055311.1"
                     /translation="MTDYRDFTPDELGQPATYHLLNAVIAPRPIAWVSTVSASGTFNL
                     APHSYTTVVSQDPPIVSFVSIGRKDSVENIEATGCFVYNIGSRAMVERINRTSADFPP
                     DISEFEWAGLTPIPGTRIAAPRVKEAPVQMETTLVEIHRVADTRNFIVMGEVVALHIA
                     ERLFDGDRVETARLDPVGRMAGSLYAAMGEVFSLKRPTYRGLLEAGESPMEPSGGR"
     gene            complement(1377..2444)
                     /locus_tag="VFI12_02555"
     CDS             complement(1377..2444)
                     /locus_tag="VFI12_02555"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7055312.1"
                     /translation="MAFTTEGFHHVTMVSRDAQRTVSFYRDVLGLRLVKKTVNFDVPN
                     TYHLYFGDDTGSPGSILTFFEWPDAVRGRYGVGGIHHIALKTANDDTLLKWKRRLTDH
                     DVPVSGPYNRGWFHSIYFRDPDGQVLEIATEGPGYDLDEPIGALGQQLKMPPEGQLRG
                     HRDEKAIAAQTWEEPVPEITPDMELRIIHHVTGFTDDLVRANEFYDQALGMRLIKQSV
                     NQDDPDTLHHFWANYDGTRIAPASSMTLFGWPETAPRASEGIGQTHHIAFRAPGDDDQ
                     EAWRTYLLGLGYEVSPVMDRKYFKSIYFRTHDGLLVEIATDGPGFAVDEDPGALGGGV
                     MLPPWLESQRDEIIAGLRPLT"
BASE COUNT          444 a          838 c          842 g          421 t
ORIGIN      
        1 ctgcacgccg agctcgcgcg acgcggccac cagccactga ctgaccgggc cacagcggtc
       61 catgccaggc aaatccaggc catcgccaac gcggccgccg atgcgatggc cccgatcgag
      121 cgcggtgagc tcccactacc cggcaagtcg gtcgcgcaca tgatcaacga ggtcttcggc
      181 gacaagacga tcctggtcaa ggagaacggc gggcaggatc tctgggtcta ctactggccc
      241 tactatcagg tctgcgagca aaactgctgt gtcgctccgg ccgagcagac ggcaatgggg
      301 tacggcgtga tcggctcgat ggcggtcaag ctcggccgcc cggatcacta cgtggtgagc
      361 acgagcggcg acggcgcgtt ccagatgtcg atccacgagc tgggcacggc cgcacagatc
      421 aaaaccccgg tgacccgggt cgtgctggac gacggcgcgt tcggctgggt gcagtggatc
      481 cagcggcgcg acctcgacga tcggatcgtg gcgactgact tcgagccgca cgtcgacctg
      541 gttgcggtgg cgaaagcgtc aaactgcgag gcggaacggc tgaccgacgc cgggcatctc
      601 cgcgcagcgc tggaacgggc caaggccgca aacgacgcgg gcaagccgta cgtcctggtc
      661 gtgccgatag atcagtccca ccaccacgcc gaattcgacc ggttccacga tttcgagccg
      721 gcgccgggct cagcgaccgc ctgacggctc catcggcgat tctccagcct cgagcagccc
      781 gcgatacgtg ggccgcttga ggctgaacac ctcgcccatc gccgcgtaga gcgagccagc
      841 catacgcccg accgggtcca ggcgggccgt ctcgacccgg tcgccatcga acagccgctc
      901 ggcgatgtgc agagcgacga cctcacccat cacgatgaag ttcctggtgt cggccacgcg
      961 atgaatctcg accagcgtcg tctccatctg gaccggcgcc tccttgacgc gcggcgcggc
     1021 gatccgggtg cctggaatcg gcgtcaggcc agcccactcg aactcgctaa tgtcgggcgg
     1081 aaaatcagcc gaggtccggt tgatccgctc gaccatcgcg cggctgccaa tgttgtagac
     1141 gaagcagccg gtggcctcga tattctcgac tgaatccttg cgcccgatgc tgacgaacga
     1201 gacgatcggc gggtcctgcg agacgacggt ggtgtaggag tgcggcgcga ggttgaacgt
     1261 accgctcgcg gagacggtcg agacccaggc aatcggccgc ggcgcgatca cggcattgag
     1321 caagtgatac gtcgccggtt gcccgagctc gtccggcgtg aagtcgcggt aatcggtcac
     1381 gtcagcggcc gcagccccgc aatgatctcg tcgcgttggg actcgagcca cggcggcagc
     1441 atcacgccac cgccgagtgc gcccgggtcc tcgtcgaccg cgaaccccgg cccgtccgtc
     1501 gcgatctcga ccagcaagcc gtcgtgcgtg cggaaataga tgctcttgaa gtacttgcga
     1561 tccatcaccg gcgacacctc gtagccgagg ccgagcaggt acgtgcgcca cgcctcctgg
     1621 tcatcgtcgc ctggagcccg aaaggcgatg tggtgcgtct ggccgatacc ctcgctcgcc
     1681 cggggggccg tctcgggcca gccaaagagc gtcatcgagc tggccggcgc aatccgtgtg
     1741 ccatcgtagt tggcccagaa gtgatggagc gtgtctgggt cgtcctgatt gaccgactgc
     1801 ttgatcagcc gcatgcccag cgcctggtcg tagaactcgt tggcgcggac caggtcgtca
     1861 gtgaagccgg tcacgtggtg gatgatgcgc agttccatgt ccggggtgat ctccgggacc
     1921 ggctcctccc acgtttgcgc ggcgatcgcc ttctcgtcgc ggtgaccccg cagctgcccc
     1981 tccggcggca tcttgagctg ttgcccgagg gcgccaatcg gctcgtcgag gtcgtagccc
     2041 ggcccttcgg tcgcgatctc gaggacctgt ccatcgggat cgcggaaata gatgctgtgg
     2101 aaccagcctc gattgtacgg gccggagacg ggcacgtcgt ggtcggtcag ccgccgcttc
     2161 cacttcagga gcgtgtcgtc gttcgcggtc ttgagcgcaa tgtggtggat accaccgacg
     2221 ccatatctcc cgcgaacggc gtccggccac tcgaagaagg tcaggatcga gccgggcgaa
     2281 cccgtgtcat cgccgaaata gaggtggtac gtgttcggga cgtcgaagtt gaccgttttc
     2341 ttgaccagcc gcagcccgag cacgtcccgg taaaacgaga cggtgcgctg ggcatcgcgc
     2401 gaaaccatcg tgacgtggtg aaagccctcg gttgtgaatg ccattggtca cctccgcgac
     2461 gcagattccg ccggccgccc tggcaggtgg tgccgacgct agtagccgcg atcaccgtag
     2521 gtgcgccggt acaaccctgc cagga
//
LOCUS       DASSGX010000142         2907 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1005928, whole genome shotgun sequence.
ACCESSION   DASSGX010000142 DASSGX010000000
VERSION     DASSGX010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1005928"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            431..1348
                     /locus_tag="VFI12_02560"
     CDS             431..1348
                     /locus_tag="VFI12_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7055313.1"
                     /translation="MNETADTPLITASAHREPRVIAIANQKGGVGKTTTAVNLAALMA
                     ERGYRVLVIDADPQGNATSSLGVDKAELAETLYDAMLDPEGVQSLPVRDVRNGVDLYP
                     ATPDLAAIEVELVAQHARERALARVLAATGADYDLCLIDCPPSLGLLTVNALTAADAV
                     LVPLQCEFLALEGLGQLLSTIDLIKRGLNPELTVLGVLMTMYDARTRLSAHVVEEVRR
                     HMPNDLLTTIVPRAIRLAEAPSYGQVISEYDPDSRGATAYEQLADELLARLGWPGRLS
                     ENDTETSGDLVATMDGGDALHDGHGFGAD"
     gene            1320..2879
                     /gene="guaB"
                     /locus_tag="VFI12_02565"
     CDS             1320..2879
                     /gene="guaB"
                     /locus_tag="VFI12_02565"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HET7055314.1"
                     /translation="MTDTVSAPTSRRTNGKFSIGDSTQTNRTMRQDKFVGLGLTFDDV
                     LLLPAASSMLPADVDTTTRIAGDIMLRVPIMSAAMDTVTEGRMAIALARAGGIGVIHR
                     NLSIEDQVAEVDKVKRSESGMIVEPVTLTPDRAVRDAVAVMDHYHISGVPITEPDGKL
                     VGILTNRDLRFIDNIDQPIGGVMTKENLITAPVGTTLDEAAAILHRHRVEKLPVVDEK
                     GYLSGLITVKDIQKKIEFPNATKDAQGRLRVAAAVGVGKDAEERAQALVEDGVDVLVV
                     DTAHGHHESVLAMVRTLKSRFNVAIMAGNIATGDAAEALIDAGVDAVKVGVGPGSICT
                     TRVVAGTGVPQITAVYECAQAAARHGIPVISDGGIQYSGDIAKAIAAGADAVMLGSLL
                     AGVDESPGEVVLYQGERFKEYRGMGSIGAMRARSFSKDRYFQEAVKGQDKLVPEGIEG
                     RVAYKGPLGNLVFQLVGGLRSAMGYVGAEDIASLKRDAQFVQITAAGLRESHPHDVFV
                     TKEAPNYRMSN"
BASE COUNT          513 a          925 c          977 g          492 t
ORIGIN      
        1 ctactggcac aacaacttcg gccacaagat gagccacggt tgcgtcaacc tgccgctcga
       61 tttcgcaacc tggctctacg gctgggcccc gctcggcact gaagtctggg tccacgagta
      121 gccggttccg gccgttcccg cgctcaggtt cgcccggcgc tagacgctaa agtaccgggc
      181 tcccccaaat aagcttgctt tagcaggctg aggcgtcggg gcactcaacg tttcgcgcca
      241 attagcctcg gagaagcttc gttgtctcag cccggagttt cgaccccggg cgccgctgcg
      301 caatcgcatg aatccacacc cgtctggtta tggatagctg ccgggtgata ggatttgggg
      361 attccggatg cgtcctcggg gctggtccgg atccggcatt tccccgatca acagacggtc
      421 gaagactgga ttgaatgaga cagccgacac cccgctgatc accgcctccg ctcaccgtga
      481 gcctcgggtc attgcgatcg ccaaccagaa gggcggcgta ggcaagacca cgaccgcggt
      541 caatctggcc gcgctgatgg ccgagcgggg ataccgggtg ctggtgatcg atgccgatcc
      601 gcagggcaac gcgacctcat cgctcggcgt cgacaaggct gagttggctg agacgctcta
      661 cgacgcgatg ctcgacccgg agggggtgca gtcgttgccg gtccgggatg tgcggaacgg
      721 tgtcgatctc tatccggcaa cgcctgatct cgctgcgatc gaggtcgaac tggtcgcgca
      781 gcacgcgcgc gagcgggcgt tggcccgggt gctggccgcg accggcgcgg actacgacct
      841 ttgcctgatc gattgcccgc cgtcacttgg cctgttgacc gtcaatgccc tgaccgccgc
      901 cgacgcggtg ctcgtcccgc tccagtgcga gttcctggcg ctggagggct tgggtcaact
      961 cctgtcgacg atcgacctga tcaagcgcgg gctgaacccg gagctgaccg tcctgggtgt
     1021 cctgatgacg atgtacgacg cccgcacccg cctctcggcc cacgtcgtcg aggaggtccg
     1081 ccggcacatg ccgaacgacc tgctgaccac gatcgtgccg cgtgctatcc ggctggccga
     1141 ggcgccaagc tatgggcagg tcatctccga gtacgatccc gattcccggg gcgcaactgc
     1201 ctacgagcaa ctggccgacg aattgttggc ccggttgggc tggccggggc gtctgtctga
     1261 aaacgataca gaaacgagtg gcgacctggt cgccaccatg gacggaggag acgcgctcca
     1321 tgacggacac ggtttcggcg ccgactagcc gccgaacgaa tggcaaattc tcgatcggcg
     1381 attcgacgca gacgaaccgc acgatgcggc aggacaagtt cgtcggtctg ggattgacgt
     1441 tcgacgacgt cctgcttctg ccggccgcct catcgatgct cccggcggac gtcgacacca
     1501 cgacccgaat cgccggcgac atcatgctgc gagtgccgat catgtccgcg gccatggaca
     1561 ccgtgaccga agggcggatg gcgatcgcgc tggcccgggc cggcggcatc ggcgtgatcc
     1621 accgcaatct ctcgattgag gatcaggttg ccgaagtcga caaggtcaag cgctccgagt
     1681 cgggcatgat cgtcgaaccc gtcacgttga cgcccgatcg ggcggtgcgc gatgcggtcg
     1741 cggtcatgga ccactatcac atctccggcg ttccgatcac cgagccagac ggcaagctgg
     1801 tcggtatcct gaccaaccgc gatctccgct tcatcgacaa cattgaccag ccaatcggcg
     1861 gcgtaatgac gaaggagaac ctgatcaccg cgccggttgg gaccacgctt gacgaggcgg
     1921 cggcgatcct ccaccgtcac cgggtcgaga agttgccggt ggtcgacgag aagggctact
     1981 tgtctggtct gatcaccgtc aaggacatcc agaagaagat cgagttcccg aacgcgacca
     2041 aggatgccca ggggcgcctg cgggtggccg cggcggtcgg tgtcggcaag gatgccgagg
     2101 agcgggcgca ggcactggtc gaggatggcg tcgatgtgct cgtcgtcgac accgcgcatg
     2161 ggcaccacga gtcggttctg gcaatggtcc gcacgctcaa gagccggttc aacgtcgcga
     2221 tcatggccgg caacatcgcg accggtgatg cggccgaggc gctgatcgac gccggcgtcg
     2281 atgcggtcaa ggtcggggtt ggtcccggct cgatctgcac gacgcgggtt gtggccggca
     2341 ccggcgtgcc gcaaatcacc gcggtctacg agtgcgcgca agccgcggcc cggcacggca
     2401 tcccggtcat cagcgatggc ggcatccagt actcgggcga catcgccaag gcgatcgcgg
     2461 ccggggcgga cgcggtcatg ctcggtagtt tgctggcggg cgtcgacgag agcccgggcg
     2521 aggtcgtcct ctaccagggc gagcggttca aggagtaccg gggtatgggc tcgatcggcg
     2581 cgatgcgggc gcggtcgttc tcgaaggacc gctacttcca ggaagccgtc aaggggcagg
     2641 acaagctcgt cccggagggg atcgagggcc gcgtcgccta caagggcccg ctcggcaacc
     2701 tggtcttcca actcgtcggc ggcctgcggt cggcgatggg ctacgtcggt gcggaggaca
     2761 ttgccagcct gaagcgcgac gcccagttcg tccagatcac ggcggccggc ctgcgcgaga
     2821 gccacccgca cgatgtcttc gtgaccaagg aagccccgaa ctaccggatg agcaactgac
     2881 ggatgagtcg tcagactttg gacttca
//
LOCUS       DASSGX010000143         2162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1441985, whole genome shotgun sequence.
ACCESSION   DASSGX010000143 DASSGX010000000
VERSION     DASSGX010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1441985"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VFI12_02570"
     CDS             complement(<1..266)
                     /locus_tag="VFI12_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine salvage family protein"
                     /protein_id="HET7055315.1"
                     /translation="MLDYTLPHDDPLGVLTSTRFVMERAESVRLDPKALARIADELGP
                     LMTAAPDWHDARHFSDGTWRTAGWVLVLDALNFCFWSQTPERWLV"
     gene            complement(444..1004)
                     /locus_tag="VFI12_02575"
     CDS             complement(444..1004)
                     /locus_tag="VFI12_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055316.1"
                     /translation="MRRWSLFVLALVAMLGVALIGRGPGIAAQDATPAAGMEGEPEGV
                     TFSPIAFGQVQHLMLPDAADLVLFRLTIDPGATFTDEGNDPSTGLVYVESGTVTFTLK
                     DTVQVLRAQSIAAMTGAPGATPMAEGASPFEEVAANTEFQLEAGDSVLIPGNVGGEAR
                     NDGDQPVSLLVANVGPPEGPEATPTS"
     gene            1239..2069
                     /locus_tag="VFI12_02580"
     CDS             1239..2069
                     /locus_tag="VFI12_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055317.1"
                     /translation="MLPRILLVLMAVALGLISLRGTAAQSTPVAGQPGCSIEPRTPEE
                     IAQIKATPVTATSPTASPVATEPVDDETLAELQRVVLMADACAAQGDYGRLAALYSTH
                     AIQSGVLDEEGVPIVPATPAATPGTSPQPGKYGPPNVSAAYRIDADHIVAEVERGPTI
                     RELRFARENGRWLIDSNEAVTGRIVEDYATPDQSSVLPMPVMQAIIDLLTTETGEQVQ
                     SITITDVQSVEWSDTFLGCPVEGSFAAQVITPGYRVQVEYKGKHYEVHTDLDGHAVTC
                     "
BASE COUNT          366 a          761 c          712 g          323 t
ORIGIN      
        1 accagccagc gctccggcgt ctggctccag aagcagaagt tgagcgcatc gaggaccagc
       61 acccagccgg ccgtccgcca ggtgccgtcc gagaagtggc gggcgtcgtg ccaatcgggc
      121 gcggccgtca tcagcgggcc gagttcgtcg gcgatccgcg ccagggcctt gggatcgagc
      181 cggaccgact ccgcccgctc catcacgaac cgcgtcgagg tcagcacgcc gagaggatcg
      241 tcgtggggga gtgtgtagtc cagcatggcg gaatgctatc aactggaccg catcatgcct
      301 cgtccgcaaa tggcgcagat aacgctcacg atggtctcct cccgaccaac caatccgcag
      361 gggatcgcgc cagccgtttg cggcgcacgg cgcggtcccc tgatggtttg gacccggcac
      421 gatagtggcg ggaggattgc tggttacgaa gtcggggtgg cctcgggacc ctccggcggt
      481 ccgacgttgg caaccaggag gctcacgggc tggtcgccgt cattgcgagc ctcgccgccg
      541 acattgcccg ggatcaggac cgaatcaccg gcctcgagct gaaactcggt gttggccgcc
      601 acctcttcga acggcgacgc gccttccgcc attggcgttg cgcccggcgc tcccgtcatt
      661 gcggcaatgc tttgcgcgcg cagcacctgc acagtgtcct tgagcgtgaa ggtaaccgtc
      721 cccgattcca cgtagaccag ccccgtagag ggatcattgc cctcatcggt gaacgtggcg
      781 ccgggatcga tggtcaagcg gaacaagacc aggtcggccg catccggcag catcaggtgc
      841 tgcacctgac cgaatgcgat cgggctaaag gtgaccccct caggctcgcc ctccatcccg
      901 gccgccgggg tggcgtcctg ggcggcgatc ccgggtccgc gcccgatcag ggcgactccg
      961 agcatggcaa ccagcgcgag aacgaacagc gaccagcgac gcatagcgcc cctcctctca
     1021 cggccgccga gggccacacg gcccgtgaca cggtggcacg cgccgcccac ggtagcgcgt
     1081 gcccggtcgg gcacctgcat aggcgctaca ggaagcggcc gaatggctcc ccaatcgggg
     1141 tgcgcgcctt gccggggatg gcggttcggg gtagggtgac gccgggttgg ggactgacgg
     1201 gcatcgcccc gaatctgacc gtgcctcagg aggtcgccat gctcccccga atcctgctcg
     1261 tactgatggc cgtcgcgctc ggcctgatct ccctgcgcgg gacggccgca caatccacac
     1321 ccgtcgccgg ccaaccgggc tgctcgatcg agccccgcac acccgaggag atcgcgcaga
     1381 tcaaggctac gccggtcacg gcgacctccc ccactgcctc gccggtagcg accgagccgg
     1441 tcgatgacga gacgctggcc gagctgcagc gcgtcgttct gatggctgat gcgtgcgccg
     1501 cccagggcga ttacggccgc ctcgccgccc tctactcaac gcatgcgatc cagagcggcg
     1561 tgctcgatga agaaggcgtg ccgatcgttc ccgccacgcc cgccgcaacc cccggcacct
     1621 cgccccagcc gggcaagtac ggcccgccga acgtcagcgc cgcctaccgg atcgacgcgg
     1681 accacattgt cgccgaggtc gagcgtggcc cgaccattcg cgagctccgc ttcgcgcggg
     1741 agaacggtcg ctggctgatc gattcgaacg aggccgttac cggccggatc gtcgaggatt
     1801 acgctacgcc ggaccagagc agcgtcctgc cgatgccggt catgcaggcg atcatcgacc
     1861 tgcttacgac ggagaccggc gagcaggtcc agtcgatcac gatcaccgac gtccagtcgg
     1921 tcgagtggtc cgacaccttc ctcggctgcc cggtcgaagg ttccttcgct gcgcaggtga
     1981 tcacgcccgg ctaccgcgtc caggtcgagt acaagggcaa gcactacgaa gtccacaccg
     2041 atctcgacgg ccacgcggtg acgtgctgac gaggcgagca ggcgagcagt tgggttgcgg
     2101 caaagtaggg gcagatggca tctgcccgcc cttcccaacc ggccccttcg aatgcaccgg
     2161 cg
//
LOCUS       DASSGX010000144         3449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_898190, whole genome shotgun sequence.
ACCESSION   DASSGX010000144 DASSGX010000000
VERSION     DASSGX010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898190"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VFI12_02585"
     CDS             complement(<1..283)
                     /locus_tag="VFI12_02585"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HET7055318.1"
                     /translation="MRRSTHLLMGAAAAAPLAALMPPVGAVGCLWLGVTGGTFPDYVD
                     LRSGMKRHLRHRGFSHSYLMLAFSTGFVYVVLLALHREPTGLLHVPLDYV"
     gene            complement(291..2987)
                     /locus_tag="VFI12_02590"
     CDS             complement(291..2987)
                     /locus_tag="VFI12_02590"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET7055319.1"
                     /translation="MATSETPTKRRTTKAAPDADQAQEQSVTEAWNAGDDFASEPATD
                     EAAAPKRTRKKKMDEPAAEAAPATNETTEAVAEEAKPKRTRKKATEEPAETAQADTEP
                     VEAAEPVAEAEAVSEIEVAPVAEAAPAADEEAAKPKRSRKKADADAEPKSLLEIAGEK
                     PSTESAAPAPQAAEAEVDEPAAEPVEEEETPVTFADLGLHDALLKSVTAVGYEEPTPI
                     QLSTIPLLLAGNDVIAQAQTGSGKTAAFGLPIIEAINPKQRNVQALILAPTRELAIQV
                     SEALHKYGRHKEVETLPIYGGQPYERQFRGLQRGPQIVVGTPGRVMDHMRRQTLDLSH
                     IKFFVLDEADEMLDMGFIEDIEWILEQVPEDRQTALFSATMPPRIVTLASRHMNQPER
                     ISVASKQMTVPEVTQAYYEVPRNRKIDALTRILDAEAPSLAMIFCRTKMMVDELGEAL
                     MARGYSAETLHGDLSQTQRDRVMRRFRTGQADILIATDVAARGLDIPDVSHVINYDIP
                     ESPETYVHRIGRTGRAGKTGVAITLVTAREVRWIRQIERAVKAKIEPRRLPTVADVAE
                     RRVEQLRNQIETALSDEAAPALAWYVSAIEGLAEEYDPTTVAAAALRLFAEETGRGPI
                     AETADDVAAVAPRPAPRERTDRFERDDRRGPRSFEPADGKVRLFMNVGHNLGIRPQDI
                     VGAIANEANIPGRSIGSIDIFDSYSFVEVPGEDSQRIIDALSQSGIKGKYVNVEVARP
                     DGGPGGGPRRDRFDRGDRGGDRGGFRGGGGGERRFDRDRPEPGNWRGRDSDRDRAPRP
                     PFDRDRPPARRDFDDRAPRFERDDRAPRFERSDRAPGFDRDPAPRFEREDRGGGGGNR
                     FERDDRGGGGNRFDRPDRFNRGPRTERPDRSDRPRRPERDRF"
BASE COUNT          493 a         1145 c         1210 g          601 t
ORIGIN      
        1 ggacatagtc gagcgggacg tggagcagcc cggtcggctc ccggtgcagg gccagcagga
       61 cgacgtagac gaagccggtg ctgaatgcca gcatcaggta gctgtgcgag aagccgcggt
      121 ggcgcaggtg gcgcttcatg ccgctgcgca gatcgacata atcggggaat gtcccgccgg
      181 tgacgccgag ccagagacag ccaacggcgc cgaccggcgg catgagagcc gccagtggcg
      241 ccgcagcagc cgcacccatc agcaggtgcg tcgatcgtcg catctatcga ctagaagcgg
      301 tcccgctccg ggcggcgcgg gcgatcggag cgatcaggtc gctcggtcct gggcccgcga
      361 ttgaagcgat ccggccggtc gaagcggttt ccaccgccgc cccggtcgtc tcgctcgaac
      421 cggttgcccc cgccgccgcc acgatcctcg cgctcgaagc gcggggccgg atcacggtcg
      481 aagcccgggg cgcgatcgct gcgctcaaac cgcggtgcgc ggtcatcccg ctcgaagcgg
      541 ggagcgcgat cgtcgaagtc gcgccgcgcc ggtggtcgat cccgatcgaa gggcggccgc
      601 ggtgcgcggt cccgatcact gtcccgaccg cgccagttgc ccggctccgg ccgatcccga
      661 tcgaagcggc gctcaccacc acctccgccc cggaagccac cccggtcgcc gccgcgatca
      721 ccgcgatcga agcgatcccg gcgcggtccg ccaccgggac cgccatcggg ccgggcgacc
      781 tcgacattga cgtacttgcc cttgatgccg ctctgcgaga gggcgtcaat gatccgctgc
      841 gagtcctcac ccggcacctc gacgaagctg tagctgtcga agatgtcgat tgagccgatc
      901 gagcggccgg ggatattcgc ctcgttcgcg atcgcgccga cgatgtcctg cggccggatg
      961 ccgaggttgt ggcccacgtt catgaagagg cggaccttgc catcggccgg ctcgaacgag
     1021 cgcggcccac ggcgatcatc gcgctcgaac cggtcggtcc gctcgcgcgg ggccggacgt
     1081 ggcgcgacgg cggcgacatc gtcggccgtc tctgcgatcg gtccgcgacc ggtctcctcg
     1141 gcgaagaggc gcagggccgc cgccgcaacc gtcgtcggat cgtactcctc ggcgagacct
     1201 tcgatagccg agacgtacca ggccagcgcg ggcgccgcct cgtcgctcag agccgtttcg
     1261 atctggttcc ggagctgctc gacccggcgc tcggcgacgt ccgccacggt cggcaggcgg
     1321 cgcggctcga tcttcgcctt gacggcgcgt tcgatctgcc ggatccagcg gacctcccgg
     1381 gcggtcacga gggtgatcgc gacgccggtc ttgccggcgc ggccggtgcg gccgatccgg
     1441 tggacgtagg tctccgggct ttccgggatg tcgtagttga tgacgtggct gacatccggg
     1501 atgtcgagac cccgggcggc gacgtcggtc gcgatcagga tatcggcctg accggtccgg
     1561 aaccggcgca tgacgcggtc gcgctgggtc tgggagaggt cgccgtggag cgtctcggcc
     1621 gaatagccgc gcgccatcag tgcctcacca agctcatcga ccatcatctt ggtgcggcag
     1681 aagatcatcg cgagggatgg tgcttccgca tcgaggatcc gggtcagggc atcgatcttc
     1741 cggttgcgcg ggacctcgta gtaggcctgc gtaacctccg ggacggtcat ctgcttgctg
     1801 gcgaccgaga tccgctcggg ctggttcatg tggcgcgagg ccagcgtgac gatccgcggc
     1861 ggcatggtgg cggagaagag ggccgtctgg cgatcctccg gcacctgctc gaggatccac
     1921 tcgatgtcct cgatgaagcc catgtccagc atctcatcgg cctcgtcgag gacgaagaac
     1981 ttgatgtggg acaggtcgag cgtctggcgg cgcatgtggt ccatgacgcg gccgggcgtg
     2041 ccgactacga tctggggacc gcgctgcaag ccgcggaact gccgctcgta cggttggccg
     2101 ccatagattg gcagcgtttc gacctccttg tggcggccgt acttgtggag cgcctcggag
     2161 acctggattg ccagctcccg ggtcggggcc aggatcaggg cctgaacgtt gcgctgcttc
     2221 gggttgattg cctcgatgat cggcaggccg aacgcggccg tcttgcctga gccggtctgg
     2281 gcctgggcga tgacgtcgtt tccggcgaga agcagcggga tcgtcgagag ctggatcgga
     2341 gtcggctctt cgtagccgac ggccgtgacg ctcttcagga gtgcgtcgtg caggccgaga
     2401 tcggcgaagg tgactggagt ctcctcctcc tcgaccggtt cggcggctgg ctcatcgacc
     2461 tcggcctccg cagcctgcgg ggcaggtgcg gccgactcgg tcgacggctt ctcgccggcg
     2521 atctcgagca ggctcttcgg ttccgcgtcg gcgtcggcct tcttgcgcga gcgcttcggc
     2581 ttggccgctt cttcgtcggc tgccggggca gcttcggcga ccggcgcaac ttcaatttcg
     2641 gataccgcct cagcttctgc gacgggttcc gccgcttcca caggctcggt atcggcctgg
     2701 gcagtttccg cgggctcctc ggttgccttc ttccgggtgc gcttcggctt cgcctcctcg
     2761 gcgacagcct cggtcgtctc gtttgtggcc ggcgcggctt ctgcggccgg ctcgtccatc
     2821 ttcttcttgc gggtccgctt cggggcggcc gcttcgtcgg tggcgggctc gctggcgaag
     2881 tcgtcgccgg cgttccacgc ctcggtcact gactgctctt gcgcctgatc ggcgtctggg
     2941 gcagccttgg tcgttcgccg cttagtcggc gtttcactcg ttgccatgga tggcatcctc
     3001 tttactcgat gccttgcgct attacggggt ccaaactcga ttccccaaaa aacccacaaa
     3061 cgtggatgga ccacggaaga gcgctcagcg cccattgggc cgcgggcgcg gccacacact
     3121 actcacaaca cccagcacac actcggaagg aacaaacctg gggatcccga gaacggccaa
     3181 ctggcggcgt tcggctgttg agtatatcag gcgagcagcc aggaggcgag caggcgagca
     3241 gcggggttaa gggttgaggg aattgacggc cggtaggggt ttgcgggggc aaactcgccg
     3301 ctcctgaccg gcccgttcac atgtggacgg gcgtggccgg gatcgcccgg gcgtcaacac
     3361 ccgggctgac atctgacccc ggctgaagcc ggctgggtgg cgcgattcca caatggcgcg
     3421 ccgccccacg gagcgcgggg aagttcttc
//
LOCUS       DASSGX010000145         3593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_245145, whole genome shotgun sequence.
ACCESSION   DASSGX010000145 DASSGX010000000
VERSION     DASSGX010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245145"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1157
                     /locus_tag="VFI12_02595"
     CDS             <1..1157
                     /locus_tag="VFI12_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HET7055320.1"
                     /translation="DAYRLWRPTPLYRAHRLEKDLGLPERVRIFYKYEGVSPSGSHKP
                     NTAVPQAFYNKQEGVKRIATETGAGQWGSSLALACQMFDLELKVYMVKISYAQKPYRR
                     ALMEAWGATCVPSPSPDTNAGRAILAQDPDSNGSLGIAISEAVEDAATRDDTKYALGS
                     VLNHVLMHQTVIGLEAIQQLEMANAWPDVVIGCTGGGSNFGGLVLPFIKHKLEGKAIR
                     IVGAEPAACPSLTKGVYAYDFGDTSKLTPLVKMHTLGHGFIPAGIHAGGLRYHGMAPL
                     VSHLFELGLMEAQAVKQTAAFESALRFARTEGILPAPEPTHAIRLAVEEALAAKESGE
                     EKTILFGLSGHGHFDLAAYDSYLSGSMQDLEYPAEAVRAAMADLPAVPVS"
     gene            1356..2135
                     /locus_tag="VFI12_02600"
     CDS             1356..2135
                     /locus_tag="VFI12_02600"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET7055321.1"
                     /translation="MPELIRSAWSALDLGAWQAVAALGAVLVGATISGLTGFGFGLVI
                     VPIMLLLFPPPTVVVLTSSLAIASGLPILLEDRAHIRVRIISPLLVPALIGLPIGVQV
                     LTGVDTRIIKLIAGVVVVIFAVLVARGFVIPGIRSRIAPVIAGLSSGILGTSTGMSGP
                     PVVLFLTDRTPEPRVFRASITFYFSLMNLVGILLVVRTGAVGGREFGIAAVLLPVALL
                     GRRIGQRLHDRVDPDQFRRITYALLILTGTSGMVTALAGLL"
     gene            complement(2132..2770)
                     /locus_tag="VFI12_02605"
     CDS             complement(2132..2770)
                     /locus_tag="VFI12_02605"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HET7055322.1"
                     /translation="MLPFDLESFIKTVSYFGVAGVVFAETGLFVGIFLPGDSLLFTAG
                     FLASQGHLNIFLLCLICFLAAVAGDATGYYIGDRMGRRLFTKPESRVFKPAYLQKAQY
                     FFEKHGGKAIIIGRFMPIVRTMVPMVAGAGTMTYHRFFTYNVVGALLWGVFIPLAGYL
                     LGSTIPSVDKYLLPIIAVIVVVSVAPSAIHIWKENGDQIKAAARRRMAERRT"
     gene            complement(2855..>3593)
                     /locus_tag="VFI12_02610"
     CDS             complement(2855..>3593)
                     /locus_tag="VFI12_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055323.1"
                     /translation="RIVSQATGEDRLNLETDRPELDHQDWAERVLGIHRARPAFTDPV
                     IRGLADRFPGMRPFSNGDLFEGIVTAIVGQSVSVQAAAVTERKLAELFAEPITVGGRS
                     YWPSPTRHQLANSEPEIVRRSGVTQRRADAIVRIARMMTENQSLLSSVDRCDEQELIQ
                     SLVNLPQVGRWTAESVLLWGLGVDDAYPIRDVALLRAARLGYDQPEMTHGDMDHLSDD
                     WRPGRSWAARLLWLNLFGPAPLSRAPD"
BASE COUNT          607 a         1145 c         1169 g          672 t
ORIGIN      
        1 gggatgccta ccggctctgg cggccgaccc cgctctatcg cgcgcaccgg ctcgagaagg
       61 atctcggcct gccggagcgg gtccgcatct tctacaagta cgagggcgtc agcccatccg
      121 gtagccacaa gccgaacacg gctgtcccgc aggcgttcta caacaagcag gaaggcgtca
      181 agcggatcgc caccgagacg ggtgccggtc aatggggcag ctccctcgcc ctcgcctgcc
      241 agatgtttga cctcgagctc aaggtctaca tggtcaagat ctcctatgcg cagaagccgt
      301 accggcgcgc gctgatggag gcatggggtg cgacctgcgt cccctccccc agtccggaca
      361 cgaacgccgg ccgcgcgatt ctggcgcagg atcccgattc gaacggttcg ctgggcatcg
      421 cgatctccga ggcggtcgaa gatgcggcca cccgggacga caccaagtac gcgctcggct
      481 cggtgctgaa ccacgtcctg atgcaccaga ccgtgattgg cctcgaagcg attcagcagc
      541 tcgagatggc caatgcctgg ccggatgtcg tgatcgggtg cacgggcggc ggttcgaact
      601 tcggcggcct ggttctgccg ttcatcaagc acaagctgga gggtaaggcg atccgcatcg
      661 tcggcgccga gccggccgcg tgcccctccc ttaccaaggg cgtctacgca tacgactttg
      721 gcgatacgtc aaagctgacg ccgctggtca agatgcacac gctcggccat ggctttatcc
      781 cggccggaat ccacgccggt ggcctccgct atcacggcat ggccccgctc gtcagccatc
      841 tcttcgaact cggcttgatg gaggcgcagg ctgtcaagca gacggccgcg ttcgagtcgg
      901 cgctccggtt cgcgcggacg gagggcatcc tcccggcgcc ggagccgacc cacgcgatcc
      961 gccttgccgt agaggaagcg ctggctgcga aggaatccgg cgaggagaag acgattctct
     1021 tcgggttgag cggccacggc cacttcgacc tggccgccta cgatagctac ctgagcggtt
     1081 cgatgcagga ccttgaatat cccgccgagg cggtccgggc cgcaatggcc gacctgccgg
     1141 ccgtcccggt gtcctgactg tcggaagatg cgcgagtatg gttcacggga aacagcgggc
     1201 gatcatcgcc cgctgtttcg tctctggagc aagacgaatg gagacgctgc aactgattcg
     1261 ccgcggcgct catcccgagt tgggtcattt ggtcatgacg ccgatgcggt tgtgcatgtg
     1321 accggttacc gtgaagaatt gcgagcgtca gtcccgtgcc ggagttgatt cgttcggcct
     1381 ggagtgcgct cgatctggga gcgtggcagg cggtggcggc gctgggcgcc gtgctggtcg
     1441 gggcgaccat ctcgggcttg accggcttcg gctttggcct ggtgatcgtg ccgatcatgc
     1501 tgttgctctt cccgccaccg acggtggtcg tcttgacctc gagcctcgcc attgcctccg
     1561 ggctgccgat cctgctcgag gaccgggcac acatccgggt ccggatcatc tcgcccttgc
     1621 tcgtgccggc gctgattggt ctcccgatcg gcgtgcaggt cctgaccggc gtcgacacgc
     1681 gcatcatcaa gctcattgcg ggcgtggtgg tcgtgatctt tgcggtgctc gtcgcccgcg
     1741 gtttcgtgat cccgggcatc cggtcgcgca tcgctccggt gatagccggg ctgtcgagtg
     1801 gtattctcgg cacctcgacg ggcatgtcgg gaccgcctgt cgtcctgttt ctgaccgatc
     1861 gcacgccgga accccgcgtt tttcgggcca gcatcacctt ctacttctcg ctgatgaatt
     1921 tggttggcat cctgctcgtc gtccggaccg gtgccgtcgg cgggcgcgag ttcgggatcg
     1981 ccgctgtgct cctgccggtt gccctccttg ggcgacgaat cggacaacgg ctgcacgaca
     2041 gagtcgatcc ggatcagttc cggcgcatca cgtacgcgct cctgatcctg accggaacgt
     2101 ccgggatggt gactgccctg gcggggttgc tctaggtgcg gcgctcggcc atccgccggc
     2161 gcgccgccgc cttgatctgg tcgccatttt ccttccagat gtggatggcc gacggtgcga
     2221 cagatacgac cacgatcacg gcgatgattg gcagcaggta cttgtccacg ctcgggatcg
     2281 tgctgcccag caggtatccg gccagcggga tgaagacacc ccagaggagc gcgccgacca
     2341 cgttgtaggt gaagaagcgg tggtaggtca tcgtgccggc gcccgcgacc atcggcacca
     2401 tggttcgcac gatcggcatg aatcggccga tgatgatcgc ctttccgccg tgcttctcga
     2461 agaagtactg cgccttctgg aggtaggccg gcttgaaaac gcgcgattcc ggcttggtga
     2521 agagccgccg gcccatccgg tccccgatgt aatagccggt ggcgtccccg gcgactgcgg
     2581 ccaggaagca gatcaggcag agcaggaaga tattcaagtg cccttgcgag gccaggaatc
     2641 cggccgtgaa gaggagactg tcgcccggaa ggaagatgcc gacgaacaac ccggtctcgg
     2701 cgaaaacaac gccggccacg ccgaagtacg agaccgtctt gatgaagctc tccagatcga
     2761 aaggcagcat gttgtcagtt tccttgttgc gagggtagtg tcgatttcaa ctgccgcgcg
     2821 gtacatggat cggcaaattc agtatagagg tgcgtcaatc aggtgcgcga gacagaggtg
     2881 cggggccgaa caggttgagc cagagcaggc gcgccgccca cgagcggccg ggacgccagt
     2941 cgtcagacaa gtggtccatg tcgccatggg tcatctcggg ctgatcgtat cccaaccgcg
     3001 ccgcccgcag gagcgctacg tcgcgaatcg gatacgcgtc atccactcct aatccccaga
     3061 gcaggaccga ttcggcagtc caccggccca cctggggcag attcaccaac gattgaatca
     3121 attcctgctc atcgcaacga tctacgctac tcagaaggga ctggttctcg gtcatcatcc
     3181 gggcgatgcg gacgatcgca tcggcccgtc gctgggtgac gccggatcgg cgcacgattt
     3241 ccggttcact atttgcgagc tggtgtcgtg tcggagatgg ccagtacgat cgacctccga
     3301 cggtgatcgg ctccgcgaag agctccgcca gctttcgctc agttacggcc gccgcctgga
     3361 cggagacgct ttgacccaca atcgccgtca cgatcccttc gaacaggtcg ccgttgctga
     3421 acggccgcat gccggggaat cggtcggcca gaccgcggat gaccggatcg gtgaatgccg
     3481 gccgggcacg atggataccg agcacgcgct ccgcccaatc ctggtggtcg agctccggcc
     3541 gatccgtctc caggttcagt cggtcctcgc cggtggcctg cgagacgatc cgg
//
LOCUS       DASSGX010000146         2275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_27585, whole genome shotgun sequence.
ACCESSION   DASSGX010000146 DASSGX010000000
VERSION     DASSGX010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27585"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..685
                     /locus_tag="VFI12_02615"
     CDS             134..685
                     /locus_tag="VFI12_02615"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HET7055324.1"
                     /translation="MFLGSREDIAHLGYVQGVCPKCHKTGSFTVYLAKRRMTISMFAA
                     LPMGEQYVLECRHCQARFAIPPEMVDQLQSRLITADRLADLVEQLPDQAEREARPVRT
                     LYQTLQVDQDADPEVIEAAFKRLAFKYHPDRSKDPDAAAKMRDVLQAKDVLADPKRRL
                     TYDRSIGIKREPPKPPALRPEDV"
     gene            complement(701..1267)
                     /locus_tag="VFI12_02620"
     CDS             complement(701..1267)
                     /locus_tag="VFI12_02620"
                     /inference="COORDINATES: protein motif:HMM:NF016085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HET7055325.1"
                     /translation="MTKSRIFYRKEKLRGACWEYQIDGGWLSVIGDHERQVLHHRFSF
                     YRPREVEVLTSDGPIMLTRWGAEQWFFPDRWFSLLRFVTESDATVGYYVNFSQPLREL
                     KANYYQDLDLELDLWLHPNGVATELDRDEFNQEIASARLLPAWAEQVNSALRTVSSAV
                     TASLAEFGPNLDAHRDPIHGLPRFILRA"
     gene            complement(1264..1998)
                     /gene="rsmG"
                     /locus_tag="VFI12_02625"
     CDS             complement(1264..1998)
                     /gene="rsmG"
                     /locus_tag="VFI12_02625"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HET7055326.1"
                     /translation="MTELAIRPELLRELPEPTQRQLRDYRDLLFRWNQRFNLTAIREP
                     EGIDSRLIADALRLLPAIDEELDRRNGKVRLIDLGTGAGLPGLVLKIARPELDIVLVD
                     ATNKKVQFVQHAIDELRLASARTIHGRAEEIGHDLDYRNQFDLVTARGVAALPTLLEL
                     TVPLLFIGGSFLFPKGHDLDEELADGKRAAKMLGARIESADLLPGVSDEPVTRLVRGS
                     KIASTPGHYPRRAGIPNKEPLGREGR"
BASE COUNT          410 a          714 c          781 g          370 t
ORIGIN      
        1 aggatgtcgc ggccgagatg accgatctct ccgccgagga gctgaaccgg ctgctcgatc
       61 cggccgatct ggcggagggc ggcatcaagg gcggcgcggg cggcggtgac tgatccggag
      121 atgaggcgcg gtcatgttcc tgggtagccg cgaggacatc gcgcaccttg gctatgtcca
      181 gggcgtctgt cccaagtgcc acaagacagg ctcgttcacg gtctacctgg ccaagcgccg
      241 gatgacgatc tcgatgttcg cggctctgcc gatgggcgag caatacgtgc tcgaatgccg
      301 ccattgccag gcgcgcttcg cgatcccgcc cgagatggtc gaccagctcc agtcgcgcct
      361 gatcacggcc gatcgactgg ccgacctggt cgagcagttg ccagatcagg ccgagcgcga
      421 ggcacgaccg gtccgcacgc tgtaccagac gttgcaggta gaccaggatg ctgatcccga
      481 ggtgatcgag gcggcattca agcgactcgc cttcaaatat cacccggacc gcagcaagga
      541 cccggatgcc gccgcgaaga tgcgcgacgt gttgcaggcg aaggacgtcc tggccgaccc
      601 gaagcgccgc ctcacctacg accgcagcat cggaatcaag cgagaaccac caaagccccc
      661 cgccctgcgc ccggaggacg tatagacgtc ctggaggggc ttaggcgcgg aggatgaagc
      721 gggggaggcc gtggatggga tcgcggtggg cgtcgaggtt gggaccgaac tcggccagtg
      781 atgcggtcac ggccgaggag actgtgcgga gcgcggaatt gacctgctcc gcccaggccg
      841 gcaggaggcg ggctgaggcg atctcctggt tgaactcgtc gcggtccagc tcagtggcga
      901 cgccgttggg gtgcagccag aggtcgagct cgaggtcgag atcctggtaa tagttggcct
      961 tcagctccct gagcggttgc gagaaattga cgtaatagcc gaccgtggca tcggattcgg
     1021 tcacgaagcg gagcagggag aaccagcgat cggggaagaa ccattgctcg gcgccccacc
     1081 gggtcagcat gatcggcccg tcggacgtca gcacctcgac ctcgcgcggg cggtagaagc
     1141 tgaagcggtg gtgcaggacc tgacgctcgt gatcgccgat cacgctcagc cagccaccat
     1201 cgatctgata ctcccagcag gcgccgcgga gcttctcttt ccggtagaaa atgcggctct
     1261 tggtcatcgg ccctccctcc caagcggttc cttgttcgga atgcccgccc gtcgtgggta
     1321 gtgtccagga gttgaagcta tcttactgcc ccggaccagc cgggtgacag gttcatccga
     1381 aacgccaggc aacaaatccg ccgattcgat gcgcgcgccg agcattttgg cggcccgttt
     1441 gccgtccgcc agctcctcgt ccagatcgtg tcccttcgga aagaggaagc tgccgccgat
     1501 gaacaggagc ggcacggtca gctccagcaa ggtcggcaac gccgccacgc cgcgagccgt
     1561 gacgaggtcg aactggttgc ggtaatcgag gtcgtgcccg atctcctcgg cgcggccgtg
     1621 gatcgtccgg gccgaggcga ggcgcagctc gtcgatcgcg tgctggacga actggacctt
     1681 cttgttggtc gcgtcgacga ggacgatgtc gagctccggc cgggcgattt tgagaaccag
     1741 gccgggcagc ccggcgccgg tgccgaggtc gatcaggcgg accttgccgt tacgtcggtc
     1801 cagctcctca tcgatcgccg gcaggaggcg gagggcatcg gcgatcaggc gcgaatcgat
     1861 tccctccggc tcgcggatcg cggtcaggtt gaaccgctga ttccaccgga agaggaggtc
     1921 gcggtagtcg cgcagttggc gctgtgtggg ttccgggagc tcgcgaagga gctccgggcg
     1981 tattgctaat tccgtcatgt cagcatccta cagcaccggg cgccgggaac gcatcgaaag
     2041 cgcaagccgg ggagggcgcg cggtgccaga tatcgagtcg ctcgctgcgt gcgcacaacg
     2101 ccgtattgat tccgcaacca ccatctggga ggagacagac caacgtggcg cgtggcttcc
     2161 acgccccgca atctgaccgt agcgcggggt taccacgccc cgcaatcgcc cgcagggcga
     2221 tgatcgttcc cgaccgaacg gtccccgatg cgcgaacgtg cgcctcgacg cggac
//
LOCUS       DASSGX010000147         2975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_245449, whole genome shotgun sequence.
ACCESSION   DASSGX010000147 DASSGX010000000
VERSION     DASSGX010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245449"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="VFI12_02630"
     CDS             complement(<1..636)
                     /locus_tag="VFI12_02630"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055327.1"
                     /translation="MTERTIRTALISPDRSFREMAKDVFLSHEAWTAPAIEIAAPFST
                     FGEDELRAVRQLRPDLVILDLADEPELGIKLAHFLSESHPGQRFVAVGPHLPPDLLLS
                     AMRAGVVDYLPRPVTPEAFQTAVERVRQQLGMGGKEAVKQPGQLYAFYSAKGGAGTTT
                     VATNFAVALQRLTGKRTLIVDLDLELGEIALLLGVQPRFNFVDMVQNFHRMD"
     gene            complement(656..1960)
                     /locus_tag="VFI12_02635"
     CDS             complement(656..1960)
                     /locus_tag="VFI12_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Tad domain-containing protein"
                     /protein_id="HET7055328.1"
                     /translation="MRTVRRRADREGGSVLALVAVSMVAMLALAAFAIDLASLRDAKA
                     ESQRAADVIALAGASAFRDLPWSEGATVDEAQTRAIAIARQNQVRADTLDIRDTASTT
                     TTYLWGSVRVVETGSNDVTLNIIPDSQKVRAWVRRPNIRGFFGYLLGWPFGHVQAMAT
                     AWATTNGPEVSCLKPFVMPDMWFESDKTTQDVNGNNYMDPITTVTGNTQDGESWKYQP
                     EEMGGKDYYIPYDPNTPPPIGKVQTGYGSGIRSLADYPSDVGLPLLLKPQTGSGNTDP
                     AAERMGNAFWMLDMDPDSNFKAEVRSMCGTAKIGDPVPYDNGSKTAVRQPINDLIQQD
                     PGAEWDNATHQVINSQFPDWTQSPRVVTVGLIDPKYWMANSSNTKPDPGSVFTNFVRI
                     FFEPVDPKGPPTNIQARYIGPAPGNGGGPNGGPLVKVLQLIE"
     gene            complement(1981..>2975)
                     /locus_tag="VFI12_02640"
     CDS             complement(1981..>2975)
                     /locus_tag="VFI12_02640"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055329.1"
                     /translation="DEDIQVSASRNTVTVSGTVKDPNSIDRALDIAKATGATVIDNLV
                     GPPAVQVLVKVRFAEINRTALRDWAVRLSTLNPHKLDADGNYSGTTNPTFGADAITFM
                     LNSGNANIQSLIQAATQKGDLRTLAEPNVMTLPGREAYFLAGGEFPYPSVQSGGGGNA
                     ISIVFKEFGIRLRFTPTIARNGAIRLKLAPEVSSLDFANGLTVEGFEIPSLRTRRAET
                     EVELREGQNLAIAGLMDNETTRNLTKIPILGDIPILGELFKSRGIRDRRTELLVVVTP
                     QLVMATDTAPPVPTGEPGPPPPSGEWKREGFLKSPEQSGSPLQGTSTPTQQPQQ"
BASE COUNT          486 a          960 c          960 g          569 t
ORIGIN      
        1 gtccatccga tggaagttct gcaccatgtc cacgaaattg aatcgcggct gcacacccag
       61 gagtagagcg atttccccca actcgaggtc gaggtctacg atcagcgtgc gcttgccagt
      121 aagcctctgc agcgcgaccg cgaagttggt ggccacggtg gtggtgccgg cgcccccttt
      181 ggcgctgtag aacgcataga gctgcccggg ctgcttgacg gcctccttcc cgcccatgcc
      241 gagctgctga cgcacccgct ccactgcggt ctgaaacgcc tccggcgtca ccggccgcgg
      301 caaataatcc accaccccgg ctcgcattgc cgagagcagg aggtcgggcg gcaggtgtgg
      361 gccgacggcg acgaatcgct gacccggatg cgactcgctc aggaagtgcg ccagcttgat
      421 tccgagctcg ggctcgtcgg ccagatcaag gatgacgaga tcggggcgga gctgccgaac
      481 tgcccggagc tcatcctccc cgaaggtgga gaacggcgcc gcgatctcaa tcgccggcgc
      541 cgtccaggcc tcgtgcgaga ggaacacatc cttggccatc tcccggaacg agcgatcggg
      601 agaaatcagt gcggtgcgga tggtccgctc ggtcatcggt accgtgccat ggcagtcact
      661 cgatcagctg caatactttg acgagtggcc ccccgttggg gcccccgccg ttgcccggtg
      721 ccggcccgat atagcgcgcc tggatgttcg tcggcggccc cttgggatcg accggctcga
      781 agaaaatgcg gacgaagttc gtgaacaccg agcccgggtc gggcttggta ttcgaagaat
      841 tggccatcca gtacttcgga tcgatgagcc ctaccgtcac cacgcggggg ctctgggtcc
      901 agtccgggaa ctgcgagttg atcacctggt gggtcgcatt gtcccattcg gcgccagggt
      961 cctgctgaat caaatcgttg atcggctgcc ggacggcggt cttgctgccg ttgtcgtagg
     1021 gaaccgggtc tccgatcttg gcggtgccgc acatgctccg aacctcggcc ttgaaattcg
     1081 agtccgggtc catgtcgagc atccagaatg cattgcccat ccgctccgcg gccggatcgg
     1141 tgttgccgga gccggtctgc ggcttgagca gcagcggcag accaacgtcg ctcgggtagt
     1201 cagccaacga ccggataccg ctcccatatc ccgtctgaac cttcccgatc gggggagggg
     1261 tattcgggtc gtaagggatg tagtagtcct taccccccat ctcctccggc tggtacttcc
     1321 acgactcgcc gtcctgcgtg ttgccggtga cggtcgtgat gggatccatg taattgttgc
     1381 cgttcacgtc ctgcgtggtc ttgtcactct cgaaccacat gtcgggcatc acgaacggct
     1441 tcaggcagct tacctccgga ccgttggtgg tggcccaggc ggtagccatg gcctgcacgt
     1501 gcccgaacgg ccacccgagg agatacccga agaagccccg aatgttcggc cgtcgcaccc
     1561 aggcgcggac cttctgactg tccgggatga tgttgagcgt tacgtcgttg gatcccgttt
     1621 cgacgacccg taccgagccc caaaggtagg tggtggtcgt cgatgccgta tcccgtatat
     1681 cgagcgtgtc cgcccggacc tggttctgcc gggcgatggc aatggctcga gtctgcgcct
     1741 cgtcgacggt ggcaccttcg gaccagggca gatcacgaaa tgcgctcgcg ccagcgagcg
     1801 cgatgacatc cgccgcccgc tgcgactccg ccttggcgtc ccggagcgac gccaggtcga
     1861 tggcgaaggc cgcgagcgcg agcatcgcca ccatcgacac ggccaccagg gccaggacac
     1921 ttccgccttc acgatctgca cgacgtcgga cggtgcgcat acgacgactc ctccgaccgc
     1981 ctactgctgc ggttgctgcg ttggcgtaga tgtcccctgc aacggactgc cggattgctc
     2041 cgggctcttc aggaagccct cacgcttcca ctcgccggaa ggcggcggcg gtcccggctc
     2101 gccggtgggc accgggggtg cggtatccgt ggccattacc agctggggtg tcacgacgac
     2161 caggagctcg gtccggcggt cgcgaatccc ccgtgacttg aacagctcac cgagaatcgg
     2221 aatgtcgccc aggatcggaa ttttggtaag attccgggtg gtctcattat ccatcagccc
     2281 ggcgattgcc aggttttgcc cctctcgaag ctcgacctcg gtttcggccc gccgggtccg
     2341 gagcgagggg atctcgaaac cctcgaccgt gagcccgttg gcgaagtcga gtgaggacac
     2401 ttcgggagca agcttgaggc gaatggcccc gttccgcgcg atcgtcgggg tgaaccgcag
     2461 ccggatcccg aactccttga acacgatgct gatcgcgttg ccccctcccc cgctctgcac
     2521 gctcggatag gggaattcgc cgcccgccag gaagtacgcc tctctgccgg gcagggtcat
     2581 cacgttgggc tcggccaagg tgcggagatc gcctttctgc gtcgccgcct ggatcagcga
     2641 ttggatgttg gcgttcccgc tgttgagcat gaacgtgatc gcgtcggcgc cgaacgtcgg
     2701 gttggtagtg ccagagtagt tcccgtcggc atcgagcttg tgcgggttga gcgtgctgag
     2761 ccgcactgcc cagtcccgaa gggcggtgcg gttgatctcg gcaaatcgca cctttaccag
     2821 cacctggacc gcgggcggcc ccaccagatt gtcgatgacg gtcgcgcccg tcgccttggc
     2881 gatgtcgagc gcgcggtcga tgctattggg gtccttgacc gtacctgaca cggttaccgt
     2941 gttccggctg gccgacacct ggatgtcctc gtcgg
//
LOCUS       DASSGX010000148         7875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_899111, whole genome shotgun sequence.
ACCESSION   DASSGX010000148 DASSGX010000000
VERSION     DASSGX010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7875
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899111"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1830)
                     /locus_tag="VFI12_02645"
     CDS             complement(<1..1830)
                     /locus_tag="VFI12_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055330.1"
                     /translation="MSRLLESGDVRLITVTGPGGVGKTRLALETVASRKSAGNVPVDV
                     VPLASIRDYRLVIRSIARAIGVPEQPGLNDIESLARGIGDSCRLIVLDNLEQVVEAGP
                     WIAELLALCPGLTLVGTSRVRLNIQGESLIPIPPLALPPSDQRVSPDSTRTVPAIALF
                     IQRARQTQPDFDLADDNCADVIEICRRLDGLPLAIELAAARLSLLSLGALRSGLGHRF
                     ELLAGGTRDNPPRLQSLVEAINWSYELLEPHEQQMLRVLSIFLGGWTVESASAVAGIP
                     VGQAFDRIASLNEKSLVRAMPSIDRQIPRFTMLETIREFGLEELAHKDEQESVRAAHA
                     AYFLAYAESISPKLDQADQGHWLDILEADHDNVRQALDWYHASGDAERGVRLAGAMFR
                     FWDTRDYLGEGRRRLEAMLALPAPVSDHAAAARATEALAELALWQADYGASEAAYTSA
                     LKLYQGNANRLGELRCLSCLALIDISTRKLDRAEQWIRTAEEIERDLDDPFARADNDY
                     ARGALQRVLGRNEDAEASMERSFQAREQLGNRSEALAALCELAEIATLRGDFETALQR
                     WGEVLDRTREVGEVWCLALYLEGVAHVLLLGYRAESACRLLGAA"
     gene            complement(2175..2660)
                     /locus_tag="VFI12_02650"
     CDS             complement(2175..2660)
                     /locus_tag="VFI12_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET7055331.1"
                     /translation="MQGRQRLLVIIGTLILTLAGGFSIMAQNTPTPSPSATASPSPSP
                     AASPSPVGTPIGLPVGNAEEGQKLVTQCLGCHSVDGSILVGPSWKDLYGHEVELEDGT
                     EVIANEAYLYQSITDPMSQIVKGFPPAMPPYNYLTDQQVADIIAYIKTLSPEGEDDNG
                     D"
     gene            complement(2671..3126)
                     /locus_tag="VFI12_02655"
     CDS             complement(2671..3126)
                     /locus_tag="VFI12_02655"
                     /inference="COORDINATES: protein motif:HMM:NF017394.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HET7055332.1"
                     /translation="MITRRTLLLGVGALGLGACGGDDKGDKDGPPKISYGSETCDRCN
                     MVIGEERHAAALKGADGNWLLFDDTGEMIVTAQDAAPGEIKAWVHDYETSKWHDATAA
                     SYVWLPSRNTPMATGIVAYGDQALAEAKAQETGGWSKPWSAVLADWTMS"
     gene            complement(3123..3953)
                     /locus_tag="VFI12_02660"
     CDS             complement(3123..3953)
                     /locus_tag="VFI12_02660"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET7055333.1"
                     /translation="MERSVITAIAARDIRSGVRNRWFMLYAVVFILLSVGFSMLAMGG
                     STLTGQPGFGRTAAGLLNLMLLMVPLIGLTIGAQSIVGERQDRSLDYLMAQPISTREI
                     FVGKYLGAAASLALLLLLGFGVSGVVMAVRGAATGLGDFLLLVLLTTLLGLAMLSVGY
                     LISSRSPQTAAALGIALTAWLVLVIVGDLGLMSSAIVMDLNPGTLLTLTLVNPLDVYK
                     LLGVDLLQTSLDVLGPAGVYANAQLGGMLTPLLAALLLVWIVAPLPVGYLLFTRKDIR
                     "
     gene            complement(3935..4837)
                     /locus_tag="VFI12_02665"
     CDS             complement(3935..4837)
                     /locus_tag="VFI12_02665"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7055334.1"
                     /translation="MTTQIADGSGGISVRNVTKYYGRWKALDDVSFEVQPGESLGMWG
                     PNGAGKTTIMRCLLGIARYEGEVRIGGFNPAKQGREARGLIGFVPQELPVSPMSVSEL
                     GRFIATLKGCPAGAEEEPIALLGLGDQGDKAVGALSGGMRQRLALALALIGTPQVLLL
                     DEPTANLDAQGRAELLDLLRRLKAQGMTLVFSSHRPEDVISMADRVLMIEGGTVQTFL
                     PAAEFRREHESGTRLVITLTNGHLHEALTALERVGVPATGSGHVVAVDVHEGRKAEVI
                     TLLAREGVDIADFEVERGKWNVQS"
     gene            complement(4834..6303)
                     /locus_tag="VFI12_02670"
     CDS             complement(4834..6303)
                     /locus_tag="VFI12_02670"
                     /inference="COORDINATES: protein motif:HMM:TIGR04247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HET7055335.1"
                     /translation="MRARNSTRLAGRLGRLLAAVGMALMLAGPVAATQPAGAADLEVC
                     ATCGVTSLADAVATVEPGATITVRGGTYPGGLVIDKPLTLVGVDNPVIDGQEKGTLIS
                     AKGADLTISGFTLRSTGSNHDREDSAIVVEGGKATITDNRIEDALFGIYLKTAHGSVV
                     RGNTILAKPVDIAMRGDGIKIWYCDDVVVEDNQASDGRDIILWYSNRGLVKGNLFNRE
                     RYGLHLMFSNDIRIEHNSLNENSIGLFIMYSRNPTVIGNTLSNNHGPSGGGIGLKDVD
                     YAVVEGNRFVNNQVGAQIDTSPREMGVENYWKDNVFAFNHTAVALQPSVRHNTFTGNN
                     FIDNGQHVSIVGGGDLKDITWAVDGRGNYWSDYAGYDANGDGVGDVPYRSQRLFDALL
                     DDEPSLRLFLFSPAAMAVDFAAKALPSVRPQMKLEDPHPLVNPVQSALLPDAKTAGNQ
                     TRVLTGITGLLFAVPAIVVVGWGRPQRGPRRTKRGASTR"
     gene            complement(6287..6949)
                     /locus_tag="VFI12_02675"
     CDS             complement(6287..6949)
                     /locus_tag="VFI12_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HET7055336.1"
                     /translation="MVATSHNQAAQVAKPAARGLSQIKVGLDARLARFFLQAGAGILL
                     IASIFYPYWEITLHAPQYPKGLFIQTFVNRMEPDRNVFEVDGLNHYIGMIKLTDAATI
                     ERAISLYAIPAVAALAMVSFLMSGIWRTLFRLPVIAYPVIFAADLFGWLYYAGHSLDP
                     HAPMSSSIKEFTPKIFGTGRIGQFHTEAGFTIGFYLAAAAAVITLVVAVWERWARHAR
                     PQ"
     gene            complement(7020..>7875)
                     /locus_tag="VFI12_02680"
     CDS             complement(7020..>7875)
                     /locus_tag="VFI12_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HET7055337.1"
                     /translation="AIDAGATQFDDFGVPILDFQSCLEAQVELGLGPLHTQYDNTGHA
                     YTSLFLDSAIAKWKLGGEGVEGGWTLIEKIPVNYNVGHLTAVEGDTVTPQGNFVVALN
                     KWAIDRFAPVGPLHPQNFQLIDITGENMQLLYDMPIGIGEPHYAQTIRAEKIQAWTTY
                     PEMGFDSLHMAKKEDAPTPGKEGVIVEGNKVTINMTSVRSHFTPDNIEVPVGSEVTWN
                     ITNIEMAQDATHGFALPGQNINLSIEPGETTTITFTADTPGVYPYYCTEFCSALHLEM
                     MGYFLIKP"
BASE COUNT         1376 a         2603 c         2528 g         1368 t
ORIGIN      
        1 ggcagcgccg agaagacggc atgcggactc tgcccgatac ccgagcagga ggacgtgcgc
       61 gactccctcc agatacaagg cgagacacca cacctcgccc acttcgcgtg tgcgatcgag
      121 cacttcgccc catcgctgaa gcgccgtttc aaagtcacct cgcagggtcg cgatctcggc
      181 cagttcgcaa agcgcggcga gggcttccga ccgatttccg agctgctccc gagcttgaaa
      241 ggagcgctcc atactggctt ccgcgtcttc gtttcgcccg agcacgcgtt gcagcgcgcc
      301 gcgcgcgtag tcattgtctg cccgggcgaa agggtcatcg aggtcgcgct caatctcttc
      361 cgctgtccgg atccattgct cggcacgatc aagtttgcgg gtgctgatat caatcagggc
      421 aaggcaactc aaacagcgca attcgcccaa ccgattggca ttgccttgat acagcttcaa
      481 tgcgctcgta taggcggctt cggaggcgcc gtaatccgct tgccacagtg caagttctgc
      541 caaagcctcg gtcgcccgag ccgccgccgc atggtccgag actggagcgg gtagggctaa
      601 catggcctcg agccgccgcc gtccctcgcc aagatagtct cgcgtatccc agaaccgaaa
      661 catggcgcca gccagccgaa cccctcgctc ggcatcgccc gacgcgtgat accagtcgag
      721 tgcctggcga acgttgtcat ggtcggcctc gaggatatcg agccagtgcc cttggtcagc
      781 ttgatcgagt ttggggctga tcgattcggc atacgcaagg aagtacgcag cgtgagcagc
      841 gcggaccgat tcctgctcgt ccttgtgcgc aagctcctcg agcccgaact cgcggatggt
      901 ctccagcatc gtgaagcggg ggatttgcct gtcgatggat ggcatcgcgc gaacgaggct
      961 cttctcgttg agcgatgcga tgcgatcgaa cgcctggccg accgggatac ccgccacagc
     1021 cgatgccgat tcgaccgtcc acccgccgag aaagatcgag aggacccgga gcatctgctg
     1081 ctcgtgcggc tcgagcagct catagctcca gttgatcgcc tccacgaggc tctgcagccg
     1141 gggtgggttg tcgcgcgtgc cgccggccag caactcgaag cggtggccga gcccggaccg
     1201 gagcgcgcca agcgacagca gcgacaaccg ggcggcggca agctcaatgg ccagcggcaa
     1261 gccgtcgagt ctgcggcata tctcgatgac gtcggcgcaa ttgtcgtcag ccagatcgaa
     1321 gtcgggctgg gtctggcggg cgcgttggat gaagagcgca atggcaggga cagtgcgggt
     1381 cgagtccggc gaaacacgct ggtcgcttgg cggcagggca aggggcggaa tgggaatgag
     1441 cgactcaccc tggatattga gccgcacccg gctggtaccg acaagggtga gcccggggca
     1501 cagcgcgagc agctcggcta tccagggccc cgcctcgacc acttgctcga ggttatcgag
     1561 cacgatgagc cggcaactgt caccgatacc gcgcgccaac gactcgatgt cgttcaatcc
     1621 cggctgctct ggaacgccga tcgcgcgggc gatgctcctg atgacgagcc gatagtcccg
     1681 gattgaggca agcggaacga cgtcgacagg gacgttgccc gccgatttgc ggctcgcgac
     1741 ggtctcgagg gcaagccgcg tcttcccgac gccaccgggc ccggtaaccg tgatgagccg
     1801 aacatcaccg gattccagga gccgggacaa ggtcgcagct tcggtttcac gcccgacaaa
     1861 cgagctaatc gggcgcggta cctggccgat ccattcggat tcacgttcac ctggcatggc
     1921 cgcctcgcac agtgagccga tcccgttctt cccatgccac acttatagtt cgcgtagtca
     1981 acagcggcat cgcacctgaa ggtcacggca ctacggatga ccgcgagcgc gcgtcgcacg
     2041 tgcattcgct gaggtggcga atgaaatgga gttcgaccgt agcgctcggg cgagaaactg
     2101 cagtatcgcg gacaagcaaa caccgcccgg tcggaaatcg accgggcggt gcgccgtctc
     2161 agatcgggaa tcggctagtc gccgttgtcg tcctcgccct caggactaag ggtcttgatg
     2221 taggcgatga tgtccgcgac ctgctgatcg gtcaagtaat tgtagggtgg catcgcaggc
     2281 gggaagccct tgacgatctg cgacattgga tcagtgatgg actggtagag atacgcctcg
     2341 ttggcgatta cctccgtgcc gtcctcgagc tcgacctcgt gcccataaag gtccttccag
     2401 ctcggcccga ccaggatact gccatcaacc gagtggcagc cgaggcactg ggtgaccagc
     2461 ttctggccct cctcggcgtt cccgaccggc aacccgatcg gcgtgccgac gggcgatggc
     2521 gaggccgcgg gcgatgggct gggcgatgcg gtcgcgctcg gcgacggcgt cggcgtgttc
     2581 tgcgccatga tgctgaagcc gcccgcaagc gtcaggatca gcgtgccaat gatcaccaac
     2641 agtcgttgtc gtccctgcat tgtcccctcc ctagctcatc gtccagtcgg ccaacactgc
     2701 cgaccagggc ttcgaccaac caccagtctc ctgcgccttc gcctcggcca gagcctggtc
     2761 tccataggcg acgattccgg tcgccatcgg cgtgttccgg ctcggcagcc agacatagga
     2821 ggccgccgtc gcatcatgcc atttcgacgt ctcgtaatcg tgcacccacg ccttgatctc
     2881 gccgggtgcc gcgtcctgcg cggtcacgat catctcgccg gtgtcatcga acaagagcca
     2941 gttgccgtcc gcccccttca gcgccgccgc gtgccgctcc tcgccgatca ccatgttgca
     3001 gcgatcgcag gtttcgctac catacgaaat cttgggcggt ccgtctttat ctcccttgtc
     3061 gtctccgcca cacgcgccga ggccaagcgc cccgacgccg agcagaagcg ttcgtcgcgt
     3121 gatcatcgaa tatccttccg ggtgaagagc aggtacccga ccggcagcgg cgcaacgatc
     3181 cagaccaaga gcagcgccgc gagcaggggc gtcagcatgc cgccgagctg cgcattggca
     3241 tagacgccgg ccgggccgag cacgtcgagc gatgtctgga gcagatcaac gccgagcagc
     3301 ttgtagacat cgagcgggtt gaccagcgtc agggtcagca gcgtgcccgg gttcaggtcc
     3361 atcacgatcg cgctgctcat caaccccaga tcgccgacga tgaccagcac cagccaggct
     3421 gtcagggcaa tcccgagcgc ggccgccgtc tgcggggagc ggctcgagat caggtagccg
     3481 acactgagca tcgccaggcc gagcagggtc gtcagcagga ccaggagcag gaagtcgccg
     3541 agcccggtcg ccgcaccgcg caccgccatg acgacgccgg agacaccgaa gccgagcaag
     3601 agcagcagcg ccagcgacgc cgccgcgccg agatacttgc cgacgaagat ctcgcgggtc
     3661 gagatcggct gcgccatcag gtaatcgagg ctgcggtcct gccgctcgcc gacgatgctc
     3721 tgggcgccga tggtcagacc gatcagcggc accatcagca gcatcaggtt gagcagcccc
     3781 gccgccgtcc ggccgaagcc gggctggccg gtcagggtcg agccgcccat tgccagcatc
     3841 gagaagccga cgctgagcag gatgaagacg accgcataga gcatgaacca gcgattgcgc
     3901 acgcccgagc ggatatcccg ggcggcgatc gccgttatga ctgaacgttc catttgcctc
     3961 gctccacttc gaaatcggcg atatcaacgc cctcgcgcgc gagcagcgtg atcacctcgg
     4021 ccttgcggcc ctcatgcaca tcgaccgcca cgacatgccc ggagccggtc gccggcacgc
     4081 ccacccgctc gagcgcggtc agcgcctcgt gcaggtggcc gttggtcagc gtaatcacca
     4141 gccgcgtgcc gctctcgtgc tcgcgccgga actcggctgc cggcaggaag gtctggaccg
     4201 tgccaccctc gatcatcaac acccggtcgg ccatcgagat cacgtcctcg ggccggtggg
     4261 agctgaagac cagcgtcatg ccctgtgcct tgagtcgccg gagcaggtcc agcagctcgg
     4321 cccgtccctg cgcgtcgagg ttggccgtcg gctcgtcgag cagcagcacc tgcggtgtgc
     4381 cgatcagggc caacgccagc gccagccgct gccgcatgcc gcccgagagc gcaccgaccg
     4441 ccttgtcgcc ctggtcgccg agcccgagca gcgcgatcgg ttcctcctcg gcgccggccg
     4501 ggcaaccctt gagcgtggcg atgaagcgac ccagctcgga aacggacatc ggcgagaccg
     4561 gcagctcctg cggcacgaag ccgatcaggc cccgcgcctc gcggccctgt ttggccgggt
     4621 tgaagccgcc gatccggacc tcgccctcgt accgggcgat cccgagcagg cagcgcatga
     4681 tcgtcgtctt gccggcgccg ttcggacccc acatgccgag cgattcaccc ggctggacct
     4741 cgaagctgac atcgtcgagc gccttccagc ggccgtaata cttcgtcacg ttccggacgg
     4801 agattccgcc ggagccgtca gctatctgcg ttgtcatctc gtgctcgctc cccgtttcgt
     4861 tcgtcgtgga ccccgctgcg gccggcccca acccacgacc acgatcgccg gcaccgcgaa
     4921 gagcagcccc gtgattccgg tcaggacgcg ggtctggttc ccggccgtct tcgcatccgg
     4981 caagagcgcg gactggaccg ggttgaccag cgggtgcggg tcctccagct tcatctgcgg
     5041 ccgcacgctc ggcagcgcct tggccgcgaa atcgactgcc atcgccgccg ggctgaacag
     5101 gaagagccgc agcgatggct cgtcgtccag cagggcgtcg aacaggcgct ggctgcggta
     5161 cggcacgtcg ccgaccccat cgccgttcgc gtcgtacccg gcgtagtcgc tccaataatt
     5221 gccgcggcca tccacggccc aggtgatgtc cttcaggtcg ccgccaccga cgatcgagac
     5281 gtgctgcccg ttgtcgatga agttgttgcc ggtgaaagtg ttgtgccgga ccgagggttg
     5341 cagcgccacg gcggtgtgat tgaaggcgaa tacgttgtct ttccagtagt tctcgacgcc
     5401 catctcgcgc ggcgaggtgt cgatttgcgc gccgacctgg ttattgacga agcggttgcc
     5461 ctcgacgacc gcgtaatcga catccttgag gccgatcccg ccgccactcg gaccgtggtt
     5521 gttcgacagc gtgttgccga tcacggtcgg attgcggctg tacatgatga acaggccgat
     5581 cgagttctcg ttcagcgagt tgtgctcaat ccgaatatcg ttgctgaaca taaggtggag
     5641 gccatagcgc tcccggttga acaggttgcc cttgacaagc ccccggttcg agtaccagag
     5701 gatgatgtcc cgcccgtcac tggcctggtt gtcctcgacc acgacatcgt cgcagtacca
     5761 gatcttgatc ccgtcgccgc gcatcgcaat gtcgaccggc ttggccagga tcgtgttgcc
     5821 acgaacgaca ctgccgtgcg cagtctttag gtagatgccg aagagggcat cctcgatccg
     5881 gttgtcggtg atcgtcgcct tgccgccctc gaccacaatc gccgaatcct cgcggtcgtg
     5941 attggagccg gtcgagcgga gggtgaagcc gctaatcgtc aggtcggcgc ccttggctga
     6001 aatcagcgtg cccttctcct gcccgtcgat gaccgggttg tcgacgccga ccagggtgag
     6061 gggcttgtcg atcaccagcc cgccggggta ggtcccgccg cggaccgtga tcgtcgcgcc
     6121 cggctcgacc gtggcgaccg cgtcggccag cgaggtcacg ccgcaggtcg cgcagacctc
     6181 gagatcggcg gccccggctg gctgagtcgc tgccaccggg ccggccagca tgagcgccat
     6241 gccgaccgcc gccaggagcc ggcccagccg accggcgagt cgggtgctat tgcgggcgcg
     6301 catgacgggc ccaccgttcc cagacggcca cgaccagggt gatcaccgcc gccgccgccg
     6361 ccaggtaaaa gccgatcgtg aacccggcct cggtatggaa ctggccgatc cggcccgtgc
     6421 cgaagatctt cggcgtgaac tctttgatcg agctgctcat cggcgcgtgc ggatcaagcg
     6481 agtggcccgc gtagtagagc cagccgaaca ggtcggccgc gaagatcacg ggatacgcga
     6541 tcaccggcaa ccggaacagc gtccgccaga tgccgctcat caggaacgaa accatcgcca
     6601 gcgcggccac ggccgggatc gcgtagagcg agatcgcccg ctcgatcgtc gcggcgtcgg
     6661 tcaacttgat catgccgatg tagtgattga ggccgtcaac ctcaaagacg ttgcgatccg
     6721 gctccatcct gttgacaaag gtctggatga agagtccctt cggatactgg ggcgcatgga
     6781 gcgtgatctc ccagtagggg tagaagatcg aggcgatcag caggataccc gccccggcct
     6841 gcaggaagaa ccgggcgagc cgcgcatcca gcccgacctt gatctggctg agaccgcggg
     6901 cggccggctt ggcgacttgg gcggcctggt tgtgtgatgt tgcgaccatt gcttgctgcc
     6961 tttccaatca gcgcgccggg acccttgcgg atcccggcgc atgaaccgca atgacggacc
     7021 tacggtttga tgaggaagta ccccatcatc tcgaggtgga gcgccgagca gaactcggtg
     7081 cagtagtacg ggtaaacacc cggggtatcg gccgtgaagg tgatcgtggt cgtctcgccc
     7141 ggctcgatgc tgaggttgat gttctggccc ggcaacgcga acccgtgggt ggcgtcctgc
     7201 gccatctcga tattcgtgat gttccaggtc acctcggacc cgaccgggac ctcgatgttg
     7261 tccggtgtga agtggctgcg gacggaggtc atgttgatcg tgaccttgtt gccctcgacg
     7321 atcacgcctt ccttgcccgg cgtgggtgcg tcctccttct tcgccatgtg cagcgagtcg
     7381 aagcccatct ccgggtaggt cgtccaggcc tggatcttct cggcccggat cgtctgcgcg
     7441 tagtgcggct ccccgatgcc gatcggcatg tcgtagagga gctgcatgtt ctcgccggtg
     7501 atgtcgatca actggaagtt ctgcggatgg agcgggccaa ccggcgcgaa ccggtcgatc
     7561 gcccacttgt tgagtgcgac cacgaagttg ccctgcggcg tcaccgtgtc gccctcgact
     7621 gccgtcaggt ggccgacgtt gtagttgacc gggatcttct cgatgagcgt ccagccgccc
     7681 tcaacacctt cgccgccgag cttccacttg gcgatcgcgc tgtcgaggaa gaggctggtg
     7741 taggcatgac cggtgttgtc gtactgcgtg tgcagcgggc caaggccgag ctcgacctgc
     7801 gcttcgaggc aggactggaa gtcgaggatc ggcaccccga agtcatcgaa ctgggtggcg
     7861 ccggcatcga tcgcc
//
LOCUS       DASSGX010000149         3102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_572265, whole genome shotgun sequence.
ACCESSION   DASSGX010000149 DASSGX010000000
VERSION     DASSGX010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572265"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1255)
                     /locus_tag="VFI12_02685"
     CDS             complement(<1..1255)
                     /locus_tag="VFI12_02685"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HET7055338.1"
                     /translation="MDRQLAGRAREPLLLGLDIGSTNVKAVVYEPDGTALSVSCVPQE
                     TTYPQASWANYDPLRLWELSCQVIRQAISGISDLSRIVSVAVTSFGEAGVLIDAAGEP
                     TTEMIAWFDRRTLDQCRHFSEQIDADRLFAICGTVVQQIMTAPKLMWHREHEPEAWAR
                     SAGFLNTADYIAFRLSGEKAQSLSLASRTGLLDLAKRDWSDELLGLAGIKPGFLPPIL
                     DGGTSLGPVTAEASAATGLPKSTLVAVGGHDHVCGALAAGAVNRGDFLDSIGTSECLF
                     VALDTLLENVAMGRQGYTLGAHARERSYIYGGLYTSGVCYDWIRRAASGGTSHEALLA
                     AAEAVAPGSLGVTFIPHLRLSNTPHMDSQARAAFIGLTTDAGTPELTRAVLEGVAFEG
                     RATIEPLLRYAGLPPLTDAVVVGGMA"
     gene            complement(1257..2099)
                     /locus_tag="VFI12_02690"
     CDS             complement(1257..2099)
                     /locus_tag="VFI12_02690"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET7055339.1"
                     /translation="MIQRQLFRSAVVWVAVVFVVIWVLSPFAWAILTTFKSGTAVYNN
                     AWIPWVQFEPTTRSWRSLFQLPVLKEALATSAVVSIAVASLAVMLAAPASYSIARMKW
                     SGRWQSGLLFGFLGQRIMPPVVLITPYMLLSARLGLRDTIQGLVVINVTFMLPLAVIV
                     IHGAFAGMPPELIEAAGMDGASTFRTFMQIAVPLAGPALIAAWVLCLAFTWNEWLYAD
                     FMTFEKVETMPVALIAVVGGGGGANVPSAMARALSMMVVPIAAALATQRFIATGLSMG
                     AVKG"
     gene            complement(2181..>3102)
                     /locus_tag="VFI12_02695"
     CDS             complement(2181..>3102)
                     /locus_tag="VFI12_02695"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET7055340.1"
                     /translation="GHLRRGGHPGMSTVTATMQRPHEDQNRATPREIVGRILLWAAII
                     VILIWTLFPLYWALASSIRTNPDSYAIKFLPWLNFDPTLSGWRQMWSLPEIKTALKNS
                     VIIGIGAATFATILGALAGYGIARFYFDRPSNGSLTTWFLSQRIIPPVVFVTPFVLFM
                     FQVGLYDKVWGLVLLNATFNLPFPIIILTQMFREVPIELEEAAQVDGASRLEIFLKIA
                     VPLVAPGIVVSWILCMAFSWNEFLFAFSTTQDKARPMSVMLVGAEQTRGVDYLFVGTR
                     MVTMMSVPIVAALLVQRYIVRGLSLGAVKG"
BASE COUNT          615 a         1054 c          962 g          471 t
ORIGIN      
        1 gggccatgcc accgacgaca accgcatcgg tcagcggcgg cagtccggcg taccggagca
       61 gcggctcgat cgtcgcgcgc ccttcgaagg cgacgccctc gaggaccgcc cgggtcagct
      121 ccggcgtccc ggcatcggtg gtcagcccga taaaggcagc ccgcgcctgc gaatccatgt
      181 gtggggtgtt gcttaggcga aggtgaggaa tgaaggtaac gccgaggctg cccggcgcga
      241 ccgcttcggc cgctgccagc aacgcctcgt ggctcgttcc acccgacgcc gcccgccgaa
      301 tccagtcgta gcagacgccc gaggtgtaaa ggccgccgta gatgtacgac cgttcccggg
      361 cgtgcgcgcc cagcgtgtac ccctggcgac ccatcgcgac gttctcgagc agcgtgtcga
      421 gcgcgacgaa gaggcattcc gacgtcccga tcgaatcgag gaaatcaccc cggttgaccg
      481 ccccggccgc cagagcgccg caaacgtgat cgtgcccgcc gaccgcgacc aacgtcgact
      541 tcggcagtcc cgtcgccgcg ctcgcttcgg ccgtgaccgg cccgagcgac gtcccgccgt
      601 ccaggatcgg cggcagaaac ccgggcttga tcccggcgag ccccagcagt tcatcactcc
      661 agtcgcgctt cgccagatcg agcaggccgg tccgcgaggc gagcgacagg ctctgcgcct
      721 tctccccgct caggcgaaag gcgatgtaat cggccgtatt cagaaagcct gccgaccgtg
      781 cccacgcctc cggctcgtgt tcccggtgcc acatcagctt tggcgcggtc atgatctgct
      841 ggaccaccgt gccgcaaatc gcgaagagtc ggtcggcatc gatctgctcc gagaaatgac
      901 ggcactggtc cagcgtgcgc cggtcgaacc aggcgatcat ctccgtcgtc ggctctccgg
      961 ccgcatcgat cagcaccccg gcctcgccaa agctggtgac ggcaaccgag acgatccggg
     1021 agagatcgga aatacctgaa attgcctgcc ggatgacctg gcagctcaac tcccacaacc
     1081 ggagcgggtc gtaattcgcc caggatgctt gcggataggt cgtttcctgc ggtacgcacg
     1141 atacggacag ggcagtccca tccggctcat agacgacggc cttgacattg gtcgatccga
     1201 tatccagccc gagcaggagc ggctcacgtg cccggccggc cagttggcga tccatctcag
     1261 cccttcaccg cgcccatgga gaggccggtc gcaatgaacc gctgcgtggc cagcgcggcc
     1321 gcgatcggta cgaccatcat cgacagtgcc cgggccatcg ccgagggcac gttcgcgcca
     1381 ccgccgcctc cgacgacggc aatcagagcc accggcatgg tctcgacctt ctcgaacgtc
     1441 atgaaatcag cgtagagcca ttcgttccag gtgaaggcca ggcacagcac ccaggccgcg
     1501 atcagcgctg gaccggcgag cggcaccgcg atctgcatga aggtccggaa ggtgctcgca
     1561 ccatccatgc cggccgcttc gatcagttcg ggcggcatcc cggcaaaggc gccgtggatc
     1621 acgatcacgg cgagcggcag catgaaggtg acgttgatga cgacgagccc ctggatcgtg
     1681 tcccgcaacc cgagccgggc cgagagcagc atgtagggcg tgatcagcac gaccggcggc
     1741 atgatgcgct ggccgaggaa accgaagagg aggccgctct gccagcgccc gctccacttc
     1801 atccgcgcga tgctgtacga cgccggcgca gccagcatga cggcgagcga cgccaccgcg
     1861 atcgagacca cggccgacgt cgccagcgcc tccttgagga ccggcaactg gaatagcgat
     1921 cgccagctcc gcgtcgtcgg ctcgaactgg acccacggga tccacgcgtt gttgtaaacc
     1981 gcggtgcccg acttgaatgt cgtcaaaatc gcccacgcga acggcgacaa cacccaaata
     2041 accacaaaca cgacggccac ccagacgacc gccgagcgga ataactgacg ctgaatcacc
     2101 caaaatccct gaaaacgaag ggaacgggcc gtcgagcccg ctcccaatca actgctcgcc
     2161 tgctcgactc ctcgcctcaa ctatcccttc accgcaccga gcgagagtcc gcgcacgatg
     2221 tagcgctgaa cgaggagcgc ggccacgatc ggcaccgaca tcatcgttac catgcgcgtg
     2281 ccgacgaaga gatagtcgac gccgcgcgtt tgctcggcgc cgaccagcat gaccgacatc
     2341 ggccgggcct tgtcctgcgt cgtcgagaag gcgaacagga attcgttcca actaaaggcc
     2401 atgcagagaa tccagctgac gacgatgccg ggcgcgacca acggcaccgc gatcttgagg
     2461 aagatctcga gccgagacgc gccatccacc tgcgccgcct cctcgagctc gatcggcacc
     2521 tcccggaaca tctgcgtcag gatgatgatc ggaaagggca ggttgaaggt cgcgttcagc
     2581 aggaccaggc cccagacctt gtcgtagagg ccgacctgga acatgaacag cacgaacggg
     2641 gttacgaaga ccaccggcgg gatgatccgc tgggacagaa accaggtcgt caggctcccg
     2701 ttgctcggtc gatcgaagta gaaacgggca atgccatatc cggcgagcgc gccgaggatc
     2761 gttgcgaagg tcgccgcgcc gatgccgatg atgacactgt tcttgagtgc cgtcttgatc
     2821 tccggcaggc tccacatctg ccgccagccg ctcagggtcg ggtcgaagtt gagccagggc
     2881 agaaatttga tcgcgtagct gtccgggttg gtccggatcg agctggccag tgcccagtag
     2941 agcgggaaga gagtccagat caggatcacg ataatcgcgg cccaaagcag aatccggccg
     3001 acaatctccc ggggcgtcgc ccgattctga tcctcgtgcg ggcgctgcat ggttgctgtt
     3061 accgtgctca tgcccggatg acctcctctt cgtagatgcc ct
//
LOCUS       DASSGX010000150         6057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_463017, whole genome shotgun sequence.
ACCESSION   DASSGX010000150 DASSGX010000000
VERSION     DASSGX010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6057
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463017"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="VFI12_02700"
     CDS             complement(<1..758)
                     /locus_tag="VFI12_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PDC sensor domain-containing protein"
                     /protein_id="HET7055341.1"
                     /translation="MRSRLPPTFAQRLILYGLSIAVPVLVMCGALVYQYSVQQYQAIQ
                     DETLAAAHELARDLDNEIQVQLLLLDSLVGILGPEMDLARLHSNARRTLVPHGSHVVV
                     HDIGGWQILNTAVPMGDPLPARYDPNRVRELVERDRPYVSDLKADLIEGQPVWIVSTP
                     IHRQSEVAGLIGIAKAPARLIQVLEQRSRPPEWAWFVFDRQGAVVASRDAQFVGQMVP
                     ERFSRVSQEPSGIRWMESADGTPIVRAHVRSSMSG"
     gene            complement(886..2334)
                     /gene="otsA"
                     /locus_tag="VFI12_02705"
     CDS             complement(886..2334)
                     /gene="otsA"
                     /locus_tag="VFI12_02705"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase
                     (UDP-forming)"
                     /protein_id="HET7055342.1"
                     /translation="MVARLIVISNRVAVPESASEASAGGLAVAVQAALSGREGIWFGW
                     SGRITEETDHQPTIRKVGGTTYVVLDLAPGDFNEYYNGFANRVLWPILHYRLDLAEFT
                     RSDLSGYQRVNNYFADRLSSILKPDDIVWVHDYHLMPLARLLRERGHQNRIGYFLHVP
                     CPPPDILTALPKHREIVGALVAFDLIGLQTENDCENLTRYLLRQGARRAPDNHHLEAN
                     GRKVTLGAFPVGIETARFERTARRAAATALIKETAESLGGRSMVMSVDRLDYSKGLPN
                     RMDAIERFLSTYSEWHGRVTFLQIAPKSRGEIHEYMEIDRQVSTLAGQINGLHGDVTW
                     TPIRYVNKAYSRAALAGLYRLAKVGLVTPLRDGMNLVAKEYVVAQNSLDPGVLVLSQF
                     AGAAEELDGALIVNPHEPEAVAAAIRFALEMPLDARQARYQRMMDVLRRNDIHDWTSR
                     FLTTLEQARPTTTPAAATAEPPPESIAARASR"
     gene            complement(2313..4109)
                     /locus_tag="VFI12_02710"
     CDS             complement(2313..4109)
                     /locus_tag="VFI12_02710"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HET7055343.1"
                     /translation="MSAPGLDYGLIGNGRIAALVDTNARIVWWCFPRFDGNPVFSRLL
                     AGDEDKGYTDVVLQDQVRAESSYDRNTAIITTTLTDAEGGCVRITDFAPRFVHYGRMF
                     HPTQIFRRIEPVSGLPRIRIRVRPTFQYGVPATARTLGSSHIRFGVVGADVMRMTTDG
                     PLSYIAEETPFALTRPITLVFGPDEPLESGIETTGRRFEYETRQWWLSWVRTLAVPYE
                     WQSAVIRAAISLKLCTFEDTGAIVAAITTSIPESPGSGRTWDYRYCWLRDAYFVVQAL
                     NHLGNTNSMESYLDYLTNAVQKAGPLRPLYGIVPGQNLEEWVAKDLAGYEGHGPVRVG
                     NAAAEQHQHDVYGAIILASQQMFVDERLERMGDIALFRRLEQHGELAWRVALQPDSGL
                     WEFRGRWRVHTHAAAMCWVACDRLSSIAQALGLPSEAETWRDRARQLRAEILELAWNP
                     KRGAFTAAFDSDDLDASSLLLAEIGLVAPSDPRFISTCEVIERELSRSGHVMRYTAED
                     DFGAPESAFLICRFWLADALAAIGRMGDARDHFVDLLRYRNSFGLMSEDVDPVSGRLW
                     GNLPQTYSMAGVVITAKRLSKNWEQAWSLASL"
     gene            complement(4106..4849)
                     /gene="otsB"
                     /locus_tag="VFI12_02715"
     CDS             complement(4106..4849)
                     /gene="otsB"
                     /locus_tag="VFI12_02715"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HET7055344.1"
                     /translation="MTLPELDFANDALFLDLDGTLIDIAPSPDDVFAPQQLVNSLEIL
                     ARRLDNAIAVVSGRPIATIDALIAPLRLPAAGVHGAEVRFETGQANISRPAPPIPNGV
                     RWLLAPLAEIPGVLVEDKTIAIAVHFRQAPSAADRVQEIVEAAVAAHDGAQLGVVPGK
                     CVFEIKHAQFSKGTAVAEFMARKPWAGRRPIFVGDDVTDEAAFAVLPRWNGIGIAVGR
                     PMPGATVGFATAAEVRGWLAGLAREECTT"
     gene            complement(5199..>6057)
                     /locus_tag="VFI12_02720"
     CDS             complement(5199..>6057)
                     /locus_tag="VFI12_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HET7055345.1"
                     /translation="DNAGGIAEMSGLPKEVRRSTDALDAVGNTTKAVTKGYAIGSAGL
                     GALVLFAAYSYDLQHFISEANREGGAAYQYFQGVQPDFALSNPYVVVGLLIGGLIPFL
                     FGGIAMTAVGRAGGAIVMEVRRQFREKPGIMKGTERPDYAAAVDLLTRAAIKEMVIPS
                     LLPVLSPIVLFLIIYFVAGSGVEGKSAAFSAVGAMLLGVIVTGLFVAISMTSGGGAWD
                     NAKKSFEDGFVDKDGAKHVKGSEAHKASVTGDTVGDPYKDTAGPAVNPLIKITNIVAL
                     LLLAVLAQF"
BASE COUNT         1075 a         1968 c         2013 g         1001 t
ORIGIN      
        1 cccgacatgc tcgaccgaac atgagcccgc acgatcggcg tcccgtcggc gctctccatc
       61 cagcgaatac ctgaaggctc ttgggacacc cggctgaatc gctccgggac catctggccg
      121 acgaattgcg catcccggct ggcgaccacc gcgccctgtc ggtcgaatac gaaccaggcc
      181 cattcgggcg gtcgcgaccg ctgctcgagc acctgtatca gccgggccgg cgccttggcg
      241 atcccgatca gcccggccac ctcgctctgg cggtgtatgg gcgtgctcac gatccagacc
      301 ggctgacctt cgatcagatc cgccttcagg tcggagacgt acggccggtc gcgctcgacg
      361 agctccctga cccgatttgg gtcgtacctg gcgggcagcg gatcgcccat cggcacggcc
      421 gtgttgagga tctgccagcc cccgatatcg tgcacgacga cgtgcgagcc atggggcacg
      481 agggtgcgcc tggcgttcga atgcagccgg gccagatcca tctcgggccc cagaatgccg
      541 accagcgaat cgagcagcag aagctggacc tgaatctcat tatcgagatc cctggccagc
      601 tcgtgcgccg cggcgagcgt ttcgtcctga atcgcttggt attgctggac cgaatattgg
      661 tagacgaggg ccccgcacat cacgaggacc gggacggcga tcgaaagtcc gtaaaggatc
      721 agccgctggg caaatgtcgg cggcaggcgt gagcgcatga acttacgggc cggtccgagc
      781 ttcgaaccgg ggtaacctgt cgcgtccgga gccgaaatgc aaccgttcag ggaagggccg
      841 cgcgcttctg tggggcggag atacgccgac ccggcgcgcc ggcagctatc gcgatgctct
      901 ggcggcgatg gactcaggcg gtggctcggc cgtcgccgct gcaggggtcg tggtgggccg
      961 cgcctgctcg agggtggtca ggaacctgga cgtccagtcg tgaatgtcgt tgcggcgcag
     1021 cacgtccatc atacgctggt agcgcgcctg gcgggcatcg agcggcatct ccagagcaaa
     1081 acggatcgcg gcggcgaccg cctccggctc atgcggattg acaatgagcg cgccatcgag
     1141 ctcctcggcc gcaccggcga actgggacag caccagaacg cccggatcga gcgaattctg
     1201 ggccaccaca tactccttcg ccacgaggtt catgccgtca cggagcggcg tcacgagccc
     1261 caccttggcc aatctgtaga ggccggcgag cgccgcccgc gagtaggcct tgttgacgta
     1321 gcggatcggc gtccaggtaa cgtcgccgtg aaggccgttg atctggccgg cgagcgttga
     1381 gacctgccgg tcgatctcca tgtactcgtg gatttcaccg cgggacttcg gtgcgatctg
     1441 cagaaacgtc acgcggccgt gccattcgga gtaggtgctg aggaatcgct cgatggcgtc
     1501 catccggttc ggcaggcctt tcgagtagtc gagccggtcg acactcatca ccatggagcg
     1561 accgcccaga ctttcggccg tttccttgat gagcgccgtt gcggctgccc gccgcgctgt
     1621 ccgttcgaag cgagccgtct cgatgccgac ggggaaggcg ccgagcgtga ccttgcgccc
     1681 gttggcttcc aggtgatgat tgtccggcgc gcgcctggcg ccctgccgca ggaggtagcg
     1741 ggtgaggttc tcgcaatcgt tctccgtctg cagcccgatg agatcgaatg cgaccagcgc
     1801 gccgacgatc tcgcgatgct tcggcagcgc ggtcaggatg tcgggtggcg ggcagggcac
     1861 gtggagaaag tagccgatcc ggttctggtg gccgcgctcg cgcagaagcc tggcgagcgg
     1921 catcaggtgg tagtcgtgca cccagacgat gtcgtcgggc ttgaggatgc tggaaaggcg
     1981 gtcggcgaaa tagttgttga cccgctgata tcccgacaga tccgaccggg tgaactcggc
     2041 gaggtcgagc cggtagtgta gaattggcca aaggacgcgg ttcgcgaacc cgttataata
     2101 ctcgttgaag tcccccggcg cgaggtcgag gacgacgtag gtggtgccgc cgaccttgcg
     2161 gatcgtgggc tggtggtccg tctcctccgt gatccggccg ctccagccga accagatgcc
     2221 ctcacgaccg ctcagtgcgg cttgtaccgc aaccgcgagg ccgccagccg aggcctcgct
     2281 ggcgctctcg gggacggcga cgcggttcga aatcacaatg aggcgagcga ccatgcctgc
     2341 tcccaattct tcgaaagccg cttggcggtg atcacgacgc ccgccatgga ataggtctgc
     2401 ggcagattac cccaaagcct tccagatacg ggatcaacat cctccgacat cagtccgaat
     2461 gaattgcgat agcgcaacag gtcgacaaaa tggtcccgcg catctcccat gcgcccgatc
     2521 gcggccagcg cgtcggccag ccagaaccgg cagatgagaa acgccgactc cggcgccccg
     2581 aagtcgtctt ctgcagtata acgcatgaca tgcccagatc ggctcaactc gcgctcgata
     2641 acttcgcaag tcgagatgaa ccgcggatcg ctgggcgcca ccaggccgat ctcggccagc
     2701 agcagcgagc tggcgtcgag gtcgtcggag tcgaaggccg cggtgaaggc gccgcgtttc
     2761 gggttccagg ccagctccag gatctccgcg cggagctggc gggcacggtc tcgccaggtt
     2821 tcggcctcac tgggcagccc gagggcttgc gcgatgctgc tcaaccggtc gcaggccacc
     2881 cagcacatcg ccgcggcgtg ggtatggacg cgccagcggc cgcggaactc ccacagcccc
     2941 gaatccggct gcagggcaac ccgccacgcc agctcgccgt gctgctcgag gcggcggaag
     3001 agggcgatgt cccccatgcg ctcaaggcgc tcgtcgacga acatctgctg tgacgccagg
     3061 atgatggcgc cgtagacgtc gtgctgatgc tgctcggcgg ccgcattgcc cacccgcacg
     3121 gggccatgcc cctcgtatcc ggcgagatcc ttggccaccc actcctcgag gttctggccg
     3181 gggacgatgc cgtagagggg ccgcagcggg ccagctttct ggacggcgtt ggtcaggtaa
     3241 tcgagataag actccatcga gttggtgttg ccgaggtggt tgagcgcctg tacgacgaaa
     3301 taggcgtcac gcaaccagca gtagcgataa tcccaggtcc gtccggaacc gggggattct
     3361 gggatcgagg tcgtgatcgc cgcgacgatg gcgccggtat cctcgaatgt gcacagtttg
     3421 agtgaaatcg cagcgcgaat gacggccgac tgccactcgt agggaaccgc gagcgtccgc
     3481 acccagctca gccaccactg gcgggtctcg tactcgaagc gccggcccgt ggtctcgatg
     3541 ccgctttcga gtggctcgtc cgggccgaac accagcgtga tcggccgggt gagcgcaaac
     3601 ggcgtctcct cggcgatata ggacagcggg ccatccgtgg tcatgcgcat cacgtcggcc
     3661 ccgacgacac cgaagcggat atgactcgat cccagcgtgc gggcagtggc cggcaccccg
     3721 tactggaacg tcggccgcac gcgaatgcgg atccgcggaa ggccggaaac cggctcgatc
     3781 cggcggaaga tctgcgtcgg gtgaaacatg cgcccgtagt gcacgaagcg gggggcgaaa
     3841 tccgtgatcc ggacgcagcc gccctcggca tccgtcagcg tcgtggtgat gatcgcggtg
     3901 ttgcggtcgt agctgctctc cgcccgcacc tgatcctgca ggacgacgtc cgtgtacccc
     3961 ttgtcctcat cgccagcgag gagacgcgag aacaccggat tgccgtcgaa gcgcggaaaa
     4021 caccaccaga cgatccgcgc gttcgtgtcg accagggccg cgatgcgacc gttgccgatg
     4081 aggccataat cgagacctgg tgcgctcacg tcgtacactc ctcacgggca agaccggcca
     4141 accagccgcg gacctctgcg gcagtggcga aacctacggt ggctcccggc attggccgac
     4201 cgactgctat tcctatgccg ttccatctcg gcaggacggc gaaggcagcc tcgtcggtca
     4261 cgtcatcgcc gacaaagatg ggccgccggc cagcccacgg ttttcgcgcc atgaactcgg
     4321 ccaccgccgt gcctttgctg aactgcgcgt gcttgatctc gaatacgcat tttccgggca
     4381 cgacgccgag ctgggcccca tcatgggccg caacggcagc ctccacgatc tcctggaccc
     4441 ggtcggcggc cgatggcgcc tgacgaaagt ggaccgcgat ggcgatcgtc ttgtcctcca
     4501 cgagcacgcc cggaatctcg gctaggggcg caagcagcca gcgcacgccg ttgggtatcg
     4561 gcggcgccgg tcgagagatg ttcgcctgtc ccgtttcgaa ccggacctcg gccccgtgca
     4621 cgcccgcggc gggtagccga agcggggcga tgagcgcgtc gatcgtggcg atcgggcggc
     4681 cggacacgac agcgatcgcg ttgtcgagcc gccgggcaag gatttcgaga gagttgacga
     4741 gctgttgcgg tgcgaagaca tcgtccggac ttggagcgat atcgatcaat gtgccgtcca
     4801 agtcgaggaa caaggcgtca ttagcgaaat cgagctctgg aagggtcatg gagatctatt
     4861 tttcggacct gggcgagcaa acgcacacag cccgcaagag caattgccct gcgggtgacg
     4921 caactgcctg ggacttggtt ccaagatggc tgccgcacga gcaaccgctg aagtcgcgcg
     4981 gacgaaatac tataggccgc gtcgggccgc aatgcgggct acacgtttga cgtgaacggg
     5041 cgacaactgg tcgtacaagt cctgaacgct gtcgcagcgg ccccgcgtag cccagcatgc
     5101 cacgtcccgg aggtgaaagc cccgccagga ttggcggggc ttgtcaggta tcgggagtac
     5161 cgcgcctgag gagctttccc ggcgcgatct ccgtgggatc agaactgcgc cagcacggcc
     5221 agcagcagca gcgccacgat attggtgatc ttgatgagcg ggttcaccgc cgggccggcc
     5281 gtgtccttgt aggggtcgcc gaccgtgtcg ccggtgaccg aggccttgtg ggcctcggag
     5341 cccttcacgt gcttcgcgcc gtccttgtcg acgaagccgt cctcgaagga tttcttggcg
     5401 ttatcccagg cgccgccgcc cgaggtcatc gagatggcca cgaacaggcc ggtgacgatg
     5461 acgccgagca gcatcgcgcc gaccgccgag aaggccgcgc tcttgccctc tacgccgctg
     5521 cccgcgacga agtagatgat caggaacagc acgatgggcg acagcaccgg cagcagcgac
     5581 gggatcacca tctccttgat cgcagccctg gtcagcaggt cgaccgccgc ggcatagtcg
     5641 ggccgctccg tgcccttcat gatgccgggc ttctcccgga actgccggcg gacctccatc
     5701 acgatggcgc cgccagcccg gccgaccgct gtcatggcga tgccgccgaa gaggaagggg
     5761 atcaggccac cgatgagcaa ccctaccacc acgtacgggt tggagagtgc gaagtccggc
     5821 tgcacgccct ggaagtactg gtacgccgcc ccgccctccc ggttcgcctc ggaaatgaag
     5881 tgctgcaggt cgtagctgta agcagcgaac agcaccaggg caccaaggcc ggccgagccg
     5941 atggcgtagc ccttcgtgac ggccttggtc gtgttgccga ccgcatcgag cgcatcggtc
     6001 gagcggcgca cctccttcgg caggcccgac atctcggcga tgccgccggc gttgtcc
//
LOCUS       DASSGX010000151         3560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1007257, whole genome shotgun sequence.
ACCESSION   DASSGX010000151 DASSGX010000000
VERSION     DASSGX010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007257"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(224..1864)
                     /locus_tag="VFI12_02725"
     CDS             complement(224..1864)
                     /locus_tag="VFI12_02725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET7055346.1"
                     /translation="MADPLAIRLCLILAFSAGTFLRLWRINAIGYNSDEAVYAGQAAA
                     LAHDPELTPFFPIIRAHPMIFQFLLALTFHWGVNDLAGRLLSAALGIATIFLVYRTGR
                     LLYGPSAGAIAALLMAVMPYHVIVTRQVLLDGPATFFTTLSLYLIAKFAATGRPWFLA
                     ATGTALGLCFMTKETAFVVFAAVYAFLALSSEIRVRIRDIILSFACMMSVMILYPISL
                     ALAGGGASEKAQGYLVWQLFRRPNHGWDFYPRFVPTAIGPALIVVALAGFFLLRHERT
                     WRETLLVTWIMVPIVVFQLWPVKGYQYLLPIAPPLAILAGRTVGVGFERLEGRRLPRL
                     LRPSLLQTVVVVLLALSLAIPAWGKVQPSQSTSLLAGSGGMPGGREAGAWIDDNIPLD
                     AIFLTIGPSMANIVQFYGHREAYGLSVSPNPLHRNPSYEPIYNPDNMIRNSELQYLVF
                     DSFSASRSSFFGDNMLLYKDKYHGRIVHQEYVTQRGSDGKMQRVPVITIYEVRPDVSS
                     SSEPPRSAEPTLKPGQGTATCGLLQAPSFWIRGARPKC"
     gene            complement(2107..3252)
                     /locus_tag="VFI12_02730"
     CDS             complement(2107..3252)
                     /locus_tag="VFI12_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055347.1"
                     /translation="MSGSVHAYDIHGIITVHSEAALPELEKFRTSARLDHATIRVRLG
                     AVKSQNGSADQANRVRYRESLGSAGFAVDIAFADDGVDVIAAPLLRFSPHVLYTNIVE
                     PILRWSFVERGYVLVHGACLAHGDDAFLITARTDTGKTTTMLRILDTQPAWSFLSDDL
                     TLLSPEGRVLTYPKPLTISRHTLVAVKTPLLSRRERFGLFFQSRLHSKSGRWFGLFLT
                     KTRLPMASTNALIQFLVPPPKYHVERLVPGVKLAREGRLAGLIVIDRHGEGQETLNDE
                     QALETLLSNCEDAYGFPPYATIKDVLYTRSGRDLRTIERETVARALSGRPATLLRSST
                     RDWWRQLPAFVNSNRTPDTSITVKVPLDTMPAVAHLETAEPAAMNAD"
     gene            complement(3255..>3560)
                     /locus_tag="VFI12_02735"
     CDS             complement(3255..>3560)
                     /locus_tag="VFI12_02735"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HET7055348.1"
                     /translation="WNFLISERFVFNRSDQKYSRVLRGAMFYAMNNAALALRVPMLFV
                     LTEALAINYVLSNVISLTLLMVLRFMSADRLIWRAPRQDPAYARLVATQGSNAEKGA"
BASE COUNT          739 a         1025 c         1153 g          643 t
ORIGIN      
        1 cgaagtagtt gtcgaacgaa tggttctcct gcattagtgt gacaaaatgc ttgatcggtg
       61 tcgtggtctg gtacgagagc gcaccgtcag tctgagctcc tgccgccggc gccaaggaag
      121 ccagggtcac gaagatggtg agaattgaga tgcttcgacg aacgctgccc ataaacgaca
      181 cctcaacgaa acggtaacgg tacccaggaa gaattacctg gaactagcac ttcggtctcg
      241 cgcctctgat ccagaatgac ggtgcctgca ggagtccgca ggtcgccgtt ccttgaccag
      301 gcttcaaggt cggctcggcc gatcggggcg gttcgctgga cgagctgacg tccgggcgaa
      361 cttcgtaaat cgtgatgacg gggacacgct gcatctttcc gtccgagcct cgttgcgtga
      421 cgtactcctg atgcacgatc cggccgtggt atttgtcctt gtaaaggagc atattgtccc
      481 cgaaaaacga cgaccgggat gcggaaaatg agtcgaacac aaggtactga agctcgctgt
      541 ttcggatcat gttgtccggg ttgtagattg gctcatacga cggattgcgg tgaagcggat
      601 tcgggctgac cgagagcccg tacgcttcgc gatggccgta aaactggacg atgttcgcca
      661 tcgacgggcc aatcgtgagg aagattgcgt cgagcgggat gttgtcgtcg atccaggctc
      721 cggcttcgcg cccgcccggc atgccgcccg accctgcaag gagcgacgtc gattgggacg
      781 gctgtacctt gccccaggcc gggatcgcga gactgagcgc taacaggacg acgacaacgg
      841 tctgaagcaa cgacggccga agcagccgcg gaagccgccg gccctcgagt cgctcgaacc
      901 ccacgcccac ggttcggccc gccagaattg ccaggggtgg cgcgatcggc aggagatact
      961 ggtacccctt cacgggccag agctggaata ccacgatcgg caccatgatc caggtgacca
     1021 gaagggtttc ccgccatgtg cgctcgtgcc ggagcaggaa gaagccggca agtgcgacca
     1081 cgatgagcgc gggcccgata gccgtcggca cgaatcgggg gtaaaagtcc cagccgtgat
     1141 tcggacgccg gaagagctgc cagaccaaat acccctgcgc cttttccgac gcaccgccgc
     1201 cggccagggc cagcgagatt gggtacagga tcattaccga catcatgcac gcgaacgaga
     1261 ggataatgtc ccgtatccga acccgaatct cggagctcag cgcgaggaac gcgtagacgg
     1321 cggcaaatac gacgaacgcg gtctccttcg tcatgaagca gagacctagc gccgtaccgg
     1381 tcgccgcgag gaaccagggt cgaccagtcg ccgcgaactt ggcgatcagg tacaggctca
     1441 atgtcgtgaa gaaggtcgcc ggtccgtcca gcagcacctg tcgggtgacg atgacgtgat
     1501 agggcatcac ggccatgagc agcgccgcga tcgcgccggc tgacgggccg tagagcaagc
     1561 ggccggttcg gtagacgagg aagatcgtgg caatgcccaa tgccgccgag agcagccggc
     1621 cggccagatc gttcacgccc cagtgaaatg tcagcgccag gaggaactga aagatcatag
     1681 ggtgggcgcg gatgatcggg aagaacggcg tgagctcggg atcatgggcg agggccgcgg
     1741 cctggcctgc gtagactgct tcgtcgctgt tgtagccgat cgcgttgatg cgccagagcc
     1801 tgaggaaggt ccctgcgctg aaggcgagaa tgaggcagag gcgaatagcc aacggatccg
     1861 ccatgaacca ggccgcgatt gaccagttgg cacgccggac cgctcggtcc gtgactaccc
     1921 gggattgggg caacgtcgat gtaagggagt gaatatcttc tgccacaatc cctgaccgtt
     1981 tcaattccaa accacctgcc gcagccgggt gggaagtaat ggagccccta ctgggcgtac
     2041 gcgatcatga ttcagcatcg gccaactttg ggtctcttct tacttgtact ggatgactct
     2101 ggaatgtcaa tccgcgttca tggcggctgg ctcggcagtt tcaaggtggg ctacggcagg
     2161 catcgtatcc agcggaacct tcaccgtgat tgaggtgtcg ggcgtccgat tgctgttcac
     2221 aaaggccggg agttggcgcc accaatcgcg cgtgctgctg cgcaggagcg tcgccggccg
     2281 gccactcagg gccctcgcga cggtctcccg ctcgatggtg cggaggtccc ggccgctgcg
     2341 ggtgtagagc acgtccttaa tggttgcgta gggcgggaag ccgtacgcgt cttcgcagtt
     2401 gctcagcaat gtttcgagcg cctgctcatc gttcagggtt tcctgtccct caccatggcg
     2461 atcaatcacg atcagcccgg ccaatcgacc ttcccgcgcc agcttcactc caggcacgag
     2521 tcgttcgacg tggtacttcg gcggcgggac aaggaactgg atcagcgcgt tggtcgacgc
     2581 catcgggagt cgtgtcttgg tcaggaatag tccaaaccac cggcccgatt tcgaatgcag
     2641 acggctctga aagaacaatc cgaaccgctc gcggcgcgaa aggagtggcg tcttgacggc
     2701 gacaagtgtg tggcggctga tcgtgagtgg cttgggatag gtcagaacgc gcccctcagg
     2761 ggagagcagt gtcaggtcgt cggagagaaa tgaccaggcg ggctgagtgt ccaggatgcg
     2821 gagcatcgtg gtggtcttgc cggtgtccgt ccgggcggtg atcaggaacg catcgtcccc
     2881 atgcgccagg cacgcgccgt gaacgagcac gtagccgcgc tcgacgaacg accaccgaag
     2941 gatgggctcg acaatattgg tatagagcac atggggcgag aagcgcagca agggcgccgc
     3001 gatgacgtct actccatcgt cggcgaaggc tatgtcgacg gcaaatccgg ccgaaccgag
     3061 actttcgcga tatcgcaccc ggttcgcctg atcggctgaa ccgttctgtg acttgacggc
     3121 gccgagccga acacggatcg tcgcgtggtc caggcgtgcg ctcgtccgga acttttccag
     3181 ttcgggcagg gccgcctcgg agtggactgt gatgatgccg tgaatgtcat acgcgtgcac
     3241 gctaccagac atcgctatgc ccctttttct gcattacttc cctgtgtggc caccaaacgc
     3301 gcataagcag ggtcctgccg cggcgcccgc cagatcaggc ggtcggcgga catgaacctc
     3361 agaaccatga ggagagttag cgaaatcaca tttgatagga cgtagttgat cgcgagcgcc
     3421 tcagtcagga cgaagagcat cgggacccgg agggcaagcg cggcgttgtt catcgcgtag
     3481 aacatggccc cacgcagcac acgactgtac ttttgatcgc tccggttgaa aacgaagcgt
     3541 tcgctgatca ggaagttcca
//
LOCUS       DASSGX010000152         3355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1117650, whole genome shotgun sequence.
ACCESSION   DASSGX010000152 DASSGX010000000
VERSION     DASSGX010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117650"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VFI12_02740"
     CDS             <1..462
                     /locus_tag="VFI12_02740"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HET7055349.1"
                     /translation="VVANRNCPGQTVISGDVGALERASELARERGAKRVARLGVSIAS
                     HSPLMEHAAAEFGQRVSELTLSDPQVPVVANGSAQPMRHRTDVAAEISAQMAAPVDWT
                     GSVQTVLAAGIRTFIELGPGTVIAGLIKRIDREATVLGISDLGLGLPSTAK"
     gene            560..1801
                     /gene="fabF"
                     /locus_tag="VFI12_02745"
     CDS             560..1801
                     /gene="fabF"
                     /locus_tag="VFI12_02745"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HET7055350.1"
                     /translation="MTKRIVVTGIGAVTPLGIGAKATWEGLLAGRSGIRKIQHWDPSA
                     LDVQIAGEVDGFVARDHMDFKLAKRMDRFAQFGVAAAREAVEHAGLTINESNADRVAV
                     VVNTGGGGIPTIESDVNKMHERGQKFVSPLLIPLFAPNMASCQVSMAFGIHGPSITSA
                     AACASGIQAFIDAYHMFQRDEADVILTGGTESGITPVAVAGLANMQALSKRNDDPAGA
                     SRPFDARRDGFVFSEGAAVFVLETEEHALARGANIIAEVHGGAYTSDAYHITAPHPEG
                     KGAATAMTRALNRANFAPTDVDYVAAHATATTIGDIAETSALKKVFGDHAYKLAISAN
                     KSMVGHLLGAAGAVSGLGCIMAIRDQMVHPTINLTTPDPQCDLDYVPNVKREMPVNVA
                     IANGFGFGGQNAVAVFSTYQS"
     gene            1953..>3355
                     /locus_tag="VFI12_02750"
     CDS             1953..>3355
                     /locus_tag="VFI12_02750"
                     /inference="COORDINATES: protein motif:HMM:NF013954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HET7055351.1"
                     /translation="MRVDRGTGKRSGWVSAALCLLMLVAAVGPAVISNRAHHAQAAVL
                     LSPTDPLLGVPRGTPEKVIRFAQAKKSTRIGDVRAYVSEVYRLSPLVGLDPALVIAQS
                     ALETGNWTSFYWTNYLNPAGIGIGYDGAPSYTWETGTLAAHAQIVRLYIYSAGAIDPG
                     NPLYPYRFDGPTYQRVIDLGYNGQAHVLDDLTGRWATDPLYGQKVASRGNGVFAPTPG
                     DPTEPAVNPVLASTSSGGDPVKLRDSDLNTAFAVLGIDSPPPGLYIDYDFGQLVTFQS
                     IQWVFRLSEYADSYQLQTSSDGVNFTTRSSYVNPTTWAWNIYGGSVTARYVRFLFQNP
                     NQDMRLGYLSEVEFYGRYANVAATATSTPYPIPTATNTPIPTNTPTVTNTPIASATAT
                     STATIAPTATPAMLSGTLLPIVGSGGSGTGNWTGYVRDGNIRTTWQTTSALPPRTAQV
                     YVDLGQSLSLTGAEFMFR"
BASE COUNT          603 a         1148 c         1049 g          555 t
ORIGIN      
        1 gtcgtcgcga accgcaattg cccggggcag acggtgatct ccggcgacgt cggagcgctc
       61 gagcgggcca gcgaactggc gagggagcgc ggggccaaac gcgtcgcgcg gctcggagtc
      121 agcatcgcct cgcactcgcc gctgatggag catgccgcgg ccgagttcgg gcagcgcgtc
      181 tcggagctca cgctcagcga tccgcaggtc ccggttgtcg cgaatggctc tgcccagccg
      241 atgaggcacc ggaccgatgt tgccgccgaa atctcggccc aaatggccgc accggtcgac
      301 tggactggca gcgtccagac cgtgcttgcc gccggtatcc gcaccttcat cgagctcggg
      361 ccgggcacgg tgatcgccgg gctgatcaaa cggatcgacc gggaagcgac ggtcctgggc
      421 atctccgacc tcggcctggg actgccatcc accgcaaaat gaaccgattt tccgcacttg
      481 agtacactat gcggcgcaac aaacgatcca gcgcgcaggg catgaacacc gccgtttgga
      541 tgtacgacgg gagagcttcg tgacaaagcg aattgtggtg acggggattg gcgcggttac
      601 cccgctgggg atcggcgcca aggcgacctg ggaagggctt ctcgctggtc gatccggcat
      661 ccgcaagatc cagcactggg atccgtcggc gcttgatgtc cagattgcgg gtgaggtcga
      721 cggcttcgtg gcccgggatc acatggactt caagctggcc aagcggatgg accgctttgc
      781 ccagttcggg gtcgcggccg cgcgggaagc ggttgagcac gccggcctga ccatcaacga
      841 gtcgaacgcg gatcgcgtcg cggtcgtggt caacaccggc ggcggcggca tcccgacgat
      901 cgaatccgac gtcaacaaga tgcacgagcg cggccagaaa tttgtcagcc cgctcctgat
      961 tccgcttttt gccccgaaca tggcgagttg ccaagtctcg atggcattcg ggatccacgg
     1021 cccgtcgatc acttcggccg cagcctgcgc ctccggcatc caggccttta tcgacgccta
     1081 tcatatgttc cagcgcgatg aggcggacgt gatcctgacc ggcggcacgg aatcgggcat
     1141 cacgccggtg gccgttgccg ggctggcgaa catgcaggcc ttgtcaaagc gtaacgacga
     1201 cccggcgggc gcgagccggc cgttcgacgc caggcgcgac ggcttcgtct tttcggaggg
     1261 ggcggcggtc tttgtcctgg agacagagga gcacgcactg gcccggggcg cgaacatcat
     1321 cgccgaggtc cacggcggcg catacacatc cgacgcctat cacatcaccg cgccgcaccc
     1381 cgaagggaaa ggcgcggcta cggccatgac ccgcgcgctc aaccgggcca acttcgcgcc
     1441 gacggacgtc gattacgtcg ccgcccatgc gacggcgacc acgatcggcg acatcgccga
     1501 gacgagcgcg ctcaagaagg tcttcgggga ccacgcctac aagctggcga tctcggccaa
     1561 caagtcgatg gtcggtcacc tgctcggcgc ggccggcgcg gtctccggcc tcggctgcat
     1621 catggctatc cgcgaccaga tggtgcaccc gacgatcaac ctgaccacgc cggacccgca
     1681 atgtgacctc gactatgtcc cgaacgtgaa acgggagatg ccggtcaacg ttgcgatcgc
     1741 gaatggcttc ggcttcggag gccagaacgc cgtcgcagtc ttctcgacat accagagcta
     1801 gctgagtatc ggggggagcg gccccttcgg tatactcggt ttgttcgttt tgcgcgcaaa
     1861 tgccgcgcgt ccatcaaatc ccgcttcccc gcgcagcaac cgaaccacca gtggcatcgc
     1921 ccgtctctcg cgtgatgccg gaaaggcgca cgatgcgggt cgatcgagga acgggcaagc
     1981 gatcaggatg ggtatcggcg gcgctgtgtc tgctgatgct cgtggccgcc gttggaccgg
     2041 ccgtcatcag caaccgagct caccatgccc aggctgcggt cctcctctcg ccgaccgacc
     2101 cgctcctggg agtgccacgc gggactcccg agaaggtcat ccggttcgcg caggcaaaga
     2161 agtcgacccg gatcggtgat gttcgggcct atgtctccga agtttatcgg ctgtcgcctt
     2221 tggtcgggct tgatccggcg ctggtgatcg cgcagtcggc cctggagacg gggaactgga
     2281 cctcgttcta ctggacgaac tacctcaacc cggccggaat cgggattggc tacgacggcg
     2341 cgccctccta cacgtgggag acggggacgc tggccgcgca cgcccagatc gtgcggctct
     2401 acatctactc ggccggcgcg atcgatcccg gtaacccgct ctacccctac cgcttcgacg
     2461 ggccgaccta ccaacgggtc atcgacctcg gctacaacgg tcaggcgcat gtcctcgatg
     2521 acctgacggg ccgctgggcc accgacccac tctatgggca gaaggtggcg agccgaggga
     2581 atggcgtttt cgccccgact ccgggcgatc cgaccgagcc ggccgtcaac cccgtcctgg
     2641 ccagcacctc gtcgggcggc gatcccgtca agctccgcga cagcgacctg aacaccgcct
     2701 tcgccgtgct cggcatcgac tcaccaccgc ccggcctcta catcgactac gacttcggcc
     2761 agctggtcac gttccagagc atccagtggg tattccggct ctccgagtat gccgattcct
     2821 accaactcca aacatcgagc gacggcgtga atttcacgac ccggtcgagc tatgtcaatc
     2881 cgacgacgtg ggcctggaat atctacggcg gttccgtaac tgcccggtac gtccggttcc
     2941 tgttccagaa cccgaaccag gacatgcggc tcggctatct ctcggaggtc gagttctatg
     3001 gtcgctatgc gaacgtcgcg gcgactgcaa cctcgacgcc ttacccgatc ccgaccgcga
     3061 ccaacacgcc gattccgaca aatacgccga ccgtgacgaa tacccccatt gccagcgcca
     3121 ccgccaccag cacggccacg attgcgccga ccgcgacgcc agccatgctt tcgggcacgc
     3181 tcctgccaat cgtcggaagc ggcggctcag gcaccggcaa ctggaccggc tacgtccgcg
     3241 acggcaacat ccgcaccacc tggcagacga ccagcgcact cccaccccgc acggcgcagg
     3301 tctatgtcga tctcggccag tcgctatccc tcaccggagc cgagttcatg ttccg
//
LOCUS       DASSGX010000153         2137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1444126, whole genome shotgun sequence.
ACCESSION   DASSGX010000153 DASSGX010000000
VERSION     DASSGX010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1444126"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..1208)
                     /locus_tag="VFI12_02755"
     CDS             complement(174..1208)
                     /locus_tag="VFI12_02755"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7055352.1"
                     /translation="MSIRLSRRTLIKSSAVVGGGLVVGRYYVGPAHAAPPPLAKKDTY
                     TVGFAQTGNTNPWRVAETASMKDQFETKLGWKLIVTDANEDTAKQLADVDSIIAQKPD
                     IFIFPPRESKALAPAVLKIKQAGIPVILIDRDVDHSVAKPGEDYVTFIGSDFVQQGMK
                     AAEWLTKAMNGTAKIIELEGTTGADPAIDRKQGFDDYIVGTFKGTPTAEGPQSGMQII
                     ASQSGNFTRDEGRKTMETLLQAHPEVTAVYAHNDEMAVGAIAALEAAGRKPGQDVTLV
                     SIDGENAAMDAIVAGKLGASVESSPFFGPISYETMMKYVNGEDLPDWVVVKDRFFDSS
                     NAKDFQGKQF"
     gene            1438..>2137
                     /locus_tag="VFI12_02760"
     CDS             1438..>2137
                     /locus_tag="VFI12_02760"
                     /inference="COORDINATES: protein motif:HMM:NF014766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HET7055353.1"
                     /translation="MVPSAAAGSEPGNQSGHMKITDVTTIKLTYQMDRAMADAIHYMP
                     ARPTLLVQVETDDGITGLGEAAAYGGSLESMQAVIGDLRQTVLGEDPFTVERLWSKMA
                     TQSHQRGRRGMLMMGISGIDIALWDIIGQATKTPLYRLLGGYRDILDAYASAGFYAKG
                     KDATELAEEVGSFVERGFRTVKIKVGRNPDVMLNPLHDMNARDYATTPFDEDIERVRA
                     ASRAAGKAKLAIDAN"
BASE COUNT          372 a          664 c          695 g          406 t
ORIGIN      
        1 ccgggaacag tttgacgatc ccgtccatgg tcagcagggg gattgcgcgc tcggcttcgt
       61 cgctcatgcc gggtctctcg cgtcgatcac gtcatgtgta catgtgcaat ggggagggat
      121 accatacggt gatccctccc cattgtccgt ctcatgtggg cggcagaccc agactagaac
      181 tgcttgccct ggaagtcctt ggcgttgctg gagtcgaaga agcggtcctt gacgacgacc
      241 cagtcaggga ggtcctcgcc attgacgtac ttcatcattg tctcgtagga gatcgggccg
      301 aagaacgggc tggattcgac cgacgcgccg agcttgccgg ccacaattgc gtccatcgcc
      361 gcgttctcgc cgtcgatcga gacgagcgtc acgtcctgac ccggcttgcg accggcggcc
      421 tcgagcgcgg cgattgcgcc gactgccatc tcatcgttgt gcgcgtagac ggcagtgact
      481 tccgggtgcg cctgcagcag cgtctccatc gtcttgcggc cttcgtcccg ggtgaagttg
      541 cccgattgtg aggcaatgat ttgcattccg ctctgcggac cttcggccgt cggcgtgccc
      601 ttgaaggtgc cgacgatgta gtcatcgaag ccctgcttgc ggtcgatcgc cggatcggcg
      661 ccggttgtgc cctcgagctc gatgatcttg gcggtgccgt tcatggcctt ggtcagccac
      721 tcggccgcct tcatgccttg ctggacgaag tcagagccga tgaaggtgac gtaatcttcg
      781 cccggtttgg ccacgctatg gtcgacatcg cggtcgatca ggatgaccgg gatgccggcc
      841 tgtttgatct tgaggacggc cggggcgagg gctttcgact cgcgcggcgg gaagatgaag
      901 atgtccggct tctgggcgat gatcgaatcg acgtcagcca gttgcttggc tgtgtcctcg
      961 ttggcgtccg tgacgatcag cttccagccg agcttggtct cgaactggtc tttcatgctg
     1021 gctgtctcag ccacgcgcca cgggttggtg ttgcccgtct gggcgaaccc gacggtgtag
     1081 gtgtccttct tggcgagcgg tgggggagcg gcgtgcgccg gtccgacgta gtaccgcccg
     1141 acgacgaggc cgccaccgac cactgcggac gatttgatca gagtgcgacg agacaggcgg
     1201 atgctcacgg caatctcctt cgttcactcc ggccggcccc cgttcgagcc ggtcgccaac
     1261 tgctccacct cgagacaacg gtgatgcgac cgctatcgca ccgtggttca ccttccgtat
     1321 acgtcgccgg acggcagacg gcctgattct gggagcacgt agcgcgattg taatcccgtg
     1381 tgaccttccg tcaagccgca gttcgctacg ataccccttg cgtgaaccga gacagccatg
     1441 gtgccgagcg ccgcggcagg cagcgagccg gggaatcaat cggggcatat gaagatcacc
     1501 gacgtcacca ccatcaagtt gacctaccag atggaccgcg caatggcgga cgcgattcac
     1561 tacatgccgg cccgcccgac ccttctcgtc caggtcgaaa ccgatgacgg aatcaccggg
     1621 cttggggagg cggcggccta cggcggatcg ctcgagagca tgcaggcggt catcggcgat
     1681 ctccgccaga ccgtgctcgg cgaggacccg ttcaccgtcg agcggctctg gagcaagatg
     1741 gcgacgcagt cccatcaacg cggccggcgc gggatgctga tgatgggcat cagcggaatc
     1801 gacatcgccc tctgggacat catcggccag gccacgaaaa caccccttta ccggctactc
     1861 ggtggctatc gggacatcct cgatgcctac gcctcggccg gcttctacgc gaagggcaag
     1921 gatgcgacgg agttggcgga agaggtcggc agcttcgttg agcgcggctt ccggacggtc
     1981 aagatcaagg tcgggcgcaa cccggacgtg atgctcaacc cgctccacga catgaacgcg
     2041 cgggactacg cgacgacacc gttcgacgag gacatcgagc gcgtccgggc ggcgagcagg
     2101 gcagccggga aggcaaagct cgcgatagac gccaaca
//
LOCUS       DASSGX010000154         2193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_138105, whole genome shotgun sequence.
ACCESSION   DASSGX010000154 DASSGX010000000
VERSION     DASSGX010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138105"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="VFI12_02765"
     CDS             complement(<1..336)
                     /locus_tag="VFI12_02765"
                     /inference="COORDINATES: protein motif:HMM:NF017973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055354.1"
                     /translation="MASEENPVEEFRRITAAAMRAIAERDDVQLSFGSEARMVGTQAR
                     LPAPPRDMTETDIAHLRGEADAMALRLRHHDDSVHKARMPASPEARAVFDTLEQVRVE
                     ALGAREMKGV"
     gene            complement(390..1397)
                     /gene="cobS"
                     /locus_tag="VFI12_02770"
     CDS             complement(390..1397)
                     /gene="cobS"
                     /locus_tag="VFI12_02770"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobS"
                     /protein_id="HET7055355.1"
                     /translation="MIGTATGNGSGGANGMGVDRPDIEVSVRQVFGLESDMVVPGFSK
                     RTEYVPDLDEAYCFDPDTTMAILVAFKHNRKLMIQGYHGTGKSTHIEQVAARLNWPCI
                     RVNLDSHISRIDLIGKDAIVLREGKQVTEFREGILPWAIQHPVALVFDEYDAGRPDVM
                     FVIQRVLEADGKMTLLDQNRVIRSHPGFRLFSTANTVGLGDTTGLYHGTQQINQAQMD
                     RWNMVATLNYLAHEKETEIVLAKVPAYDDAEGRKTVSSMVRVAELTRAGFMAGDISTV
                     MSPRTVITWAENARIFGDLGVAYRMTFLNKCDELERSTVAEYYQRCFGEELPEYQTAH
                     A"
     gene            complement(1575..>2193)
                     /locus_tag="VFI12_02775"
     CDS             complement(1575..>2193)
                     /locus_tag="VFI12_02775"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HET7055356.1"
                     /translation="ERAVALMIVPTLLTNAWQIWVNRAYATMFPNMGIVVVVSIVGVS
                     AGSSILASVPERWLFVMMAAWLGLYLVSVALRFEPNLEGWHGRWPSFVVVFFGGLVQG
                     ATGAAGSVLAPYVHALGLRKEAYVFAISVLFGLFGLVQLATLTAHGILTMDRVYEGLV
                     ACVPVAVVMPLAMRLGGRFSVRAFELLLLAVFVLMEARLIYKIVA"
BASE COUNT          398 a          801 c          646 g          348 t
ORIGIN      
        1 gacgcccttc atctcgcgcg cgcccagcgc ctcgacgcgc acctgctcca gcgtgtcgaa
       61 caccgcccgt gcctcgggcg aggccggcat ccgcgccttg tgcaccgagt cgtcatggtg
      121 acgcagccgc aaggccatcg cgtcggcctc gccgcgcaga tgggcgatgt cggtctccgt
      181 catgtcgcgc ggcggcgccg gcagccgcgc ttgcgtgccg accatccgcg cctccgagcc
      241 gaaggagagc tgcacgtcgt cgcgctcggc aatggcgcgc atcgcggcgg cggtgatccg
      301 ccggaattcc tccacgggat tttcttcgga cgccatcgct caggtcccgg cagtcatgat
      361 agtcgtgata atggggcggc gcattccgct cacgcgtgtg cggtttggta ttcgggaagc
      421 tcctcgccga agcagcgctg gtagtattcc gccaccgtcg agcgctccag ctcgtcgcac
      481 ttgttcagga acgtcatccg gtacgccacc ccgagatcgc cgaagatgcg cgcgttctcc
      541 gcccaggtga ttaccgtccg cgggctcatc accgtcgaga tgtcgcccgc catgaacccc
      601 gcccgcgtca gctccgccac ccgcaccatc gacgacaccg tcttgcggcc ctcggcatcg
      661 tcgtaggccg gaaccttcgc cagcacgatc tccgtctcct tctcgtgcgc caggtagttc
      721 agcgtcgcca ccatgttcca gcggtccatc tgcgcctggt tgatctgctg cgtgccgtga
      781 tacagacccg tcgtgtcccc gagacccacc gtgttcgcgg tcgagaacag ccggaagccg
      841 ggatgcgacc ggatcacccg gttctggtcc agcaacgtca tcttgccgtc cgcctccagc
      901 acccgctgga tcacgaacat cacgtccggg cggcccgcgt catattcgtc gaacaccaag
      961 gccaccggat gctggatcgc ccacggcagg ataccctccc ggaactccgt cacctgcttg
     1021 ccttcccgca gcacgatcgc gtccttgccg atcaggtcga tccgcgagat gtggctgtcc
     1081 aggttcaccc ggatgcacgg ccagttcagg cgcgccgcaa cctgctcgat atgcgtcgac
     1141 ttccccgtcc cgtgatagcc ctggatcatc agtttccggt tgtgcttgaa cgccaccaga
     1201 atcgccatcg tcgtgtcggg gtcgaagcag taggcctcgt ccaggtccgg cacgtactcc
     1261 gtccgcttgc tgaaccccgg cacaaccatg tccgattcca gcccgaacac ctgccgcacc
     1321 gatacctcga tgtccggccg gtcgacgccc atcccgttcg cgccgcccga cccgttcccg
     1381 gttgccgtcc cgatcaaagc tcaagctcct tccgccgaaa gcccgaattc cgccgccgag
     1441 ctcccagagc cgcgttggtg acgctcgatg ggatgtcgcc gaaaagaaat ggagggcgaa
     1501 ggccctcccc tcaggcacgg cgggagtata ataatcgcat cgggtcaaaa caagccggcc
     1561 cgccgaagcc ggattcaggc aacgatcttg tagatcagcc gcgcttccat caggacgaac
     1621 acggcgagca ggagcagctc gaatgcccgc acgctgaagc gcccgccgag gcgcatggcg
     1681 agcggcatga ccaccgccac cggaacgcag gccaccagcc cttcatagac ccggtccatg
     1741 gtcaggatgc cgtgcgcggt gagcgtcgcc aactggacca gaccgaacag gccgaacagc
     1801 accgagatcg cgaagacata ggcctccttg cgcagcccga gcgcatgcac atagggcgcg
     1861 aggacggagc ccgccgcacc ggtcgccccc tgcaccagcc cgccgaagaa cacgacgacg
     1921 aaggacggcc agcgtccatg ccagccttcc agattcggct cgaagcgcag cgcgaccgag
     1981 acgagataga gcccgagcca ggccgccatc atcacgaaca gccagcgctc cggcaccgag
     2041 gcgaggatcg acgatccggc gctgacgccg acgatcgaga ccacgaccac gatgcccata
     2101 ttgggaaaca tcgtcgcata ggcgcgattg acccagatct gccacgcatt ggtcagcagc
     2161 gtcggaacga tcatcagcgc gacggcacgc tcg
//
LOCUS       DASSGX010000155         2875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_900731, whole genome shotgun sequence.
ACCESSION   DASSGX010000155 DASSGX010000000
VERSION     DASSGX010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900731"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(435..2627)
                     /locus_tag="VFI12_02780"
     CDS             complement(435..2627)
                     /locus_tag="VFI12_02780"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET7055357.1"
                     /translation="MYRHRFLVVAIWAIAFMASIPMLLRVDEPLKVGGFSSDRTEAAR
                     ARQTVERELSGSASQLVVIFKSTDMPITNDVAQSEIASALQPLQSAPHVISTLLPSEN
                     SAQISRDGKTAYALVSLNLSAEEAQRLIPDFQRLVVPQPGMEILLAGGPAFYADIETV
                     SQRDLQRAELIAFPFALVALLFVFGTVVAAVVPLIVGGLGVAAVLMAIFGVAHVTDLS
                     IFVLNLSTMLGLGLAIDYSLFITSRFREELPRAGSVEQAVERTMATAGRAIFFSGLTV
                     LIGLSGLIMFDFMFLRSVGIAGVIVVFFSVLAALTLLPALLAIAGTRIEQWSVIRRSP
                     EDHHHLGFWARLSRGVMKRPILILVPTTLILLAFGAPFRHVNISSPDATILPKSTESR
                     QGFDELVRAFGPGEISPMVVVFQSPSSVFSRENVSAIHGFVTELQADHRVVRIEGFTA
                     FSQEISDVQAYALVQIQKRAADSGLGGRFREIANDHTAMLLVYTDSYPNSVESKDLLS
                     KIRGYPVNGDVSMLVDGGTAEIVDVVNAMYGAFPLAVAFVLLATYIVLFLLFHSVFLP
                     LKAIVMNTLSLLASYGALVWVFQDGHLERLLRFESLGYVEASLPIIMFCILFGLSMDY
                     EVFLLSRVREEWERTGDNTEAVALGLQRSGRIISSAALIVVVVAISFVSADVILVKAL
                     GFGIALAVFLDATIVRALLVPATMRLMGHWNWWRPAALERFAIPDLHE"
BASE COUNT          639 a          895 c          883 g          458 t
ORIGIN      
        1 gaagaggcgg ccggtggagg tgtactggac cacccagccc tcgccatcct tgctcagcgt
       61 gatctcgaat cgaccgtgct tgctgacaaa gatctcactg tggggagccc ccgccgggcg
      121 cacaatgtgc tcgtcaattg ggtccatgct gatggttgaa gaggaggaac gccagccaga
      181 agagatcact acatccattc gcccaaagcc cttcaaggca accgctcacg cggttttctg
      241 acaacctgtc accgcagccg ggcaaaggac ggatgaccca ccacaaccat gggcataaca
      301 ccgaacctct caacagctgc ggtgacaatg tagcacagca atgggggcca tacaattcaa
      361 catttgttcg atttttgatt cgagagaagc ccggaccacg gccaggcaac acggcgtgtt
      421 gtcgtcaaca acagttactc gtggaggtct ggaatcgcga accgctccag cgccgccggc
      481 cgccaccagt tccagtgtcc catcagccgc atcgtcgccg gaaccaggag cgcgcgcacg
      541 atcgtcgcat ccagaaagac cgccagcgcg atcccgaaac cgagcgcctt gaccaggatc
      601 acgtcggcgg agacgaacga gatcgcaacc acgacgacga tcagggccgc gcttgagatg
      661 atccgcccgc tgcgctgcaa tccaagcgcg accgcttcag tattatcgcc ggtccgctcc
      721 cactcctcgc gcacccggct gagcaagaag acctcgtaat ccatgctcag gccgaagagg
      781 atgcagaaca tgatgatcgg cagcgaggct tcaacatacc caagcgactc gaaccggagc
      841 agccgctcca ggtgcccgtc ctggaacacc caaaccagcg cgccatacga ggcgagcagc
      901 gacagcgtgt tcatgacgat cgccttcaac ggcaggaaga ccgagtggaa caagaggaag
      961 agaacgatgt acgtggcgag caggacgaac gccaccgcaa gcgggaatgc accgtacatc
     1021 gcgttcacga cgtccacgat ctcggccgtg ccgccgtcga cgagcatcga aacgtcccca
     1081 ttcaccggat agccgcgaat cttcgacaac aggtccttgc tttcgacact gttgggatag
     1141 gaatcggtgt agaccaggag catggcggtg tggtcgttcg caatttcccg gaatcggccg
     1201 cccaatccgc tgtcggctgc gcgcttctgg atttggacca gggcgtaagc ctggacatcc
     1261 gagatctcct ggctgaacgc cgtaaagccc tcgatacgca cgacccggtg atctgcctgc
     1321 agctcagtga cgaaaccgtg gatcgctgaa acgttctcgc gactgaagac cgaggacggc
     1381 gactggaaca cgactaccat cggcgagatc tcgcccggac cgaacgcccg caccagctcg
     1441 tcgaagccct gccgcgattc ggtgctcttc ggcaggatcg tcgcgtcggg ggaggagata
     1501 ttgacgtgcc ggaatggggc gccaaacgcg aggaggatca acgtcgtcgg cacgagaatg
     1561 agaatcggcc gcttcatgac cccgcgtgaa agccgcgccc agaacccgag gtggtggtga
     1621 tcctccggcg atcgccgaat gactgaccac tgctcgatcc tggtcccggc gatggcgagc
     1681 agcgccggca gaagcgtcag cgcggcaagc accgagaaga agaccacgat gacgcctgcg
     1741 atccccaccg agcgcaggaa catgaagtcg aacatgatca ggccggacag gccgatcagc
     1801 accgtcagac ccgagaagaa gatcgcccgg cccgccgtcg ccatcgtccg ctcgacggcc
     1861 tgctctaccg atccggcgcg tggcagctct tcgcgaaacc ggctggtgat gaagagcgag
     1921 tagtcgatcg ccagaccgag cccgagcatg gtcgagaggt tgagtacgaa gattgagagg
     1981 tcggtcacgt gcgcgacgcc gaagatggcc atcagcaccg ccgccacacc gaggccgccg
     2041 acgatcaggg gcacgactgc ggcgacgacc gtcccgaaga cgaacagcag cgcgaccaac
     2101 gcgaacggga acgcgatcaa ctcggcacgc tgcaagtcgc gctggctgac ggtttcgatg
     2161 tctgcgtaga aggcgggacc accggcgagc aaaatctcca tcccgggttg cggcacgacg
     2221 agccgctgga agtccgggat cagccgctgt gcttcctccg ccgagagatt gaggctgacc
     2281 agcgcgtagg cggtctttcc gtcgcggctg atctgtgcgg agttctccga gggcagaagt
     2341 gtgcttatga catgcggcgc ggattgaagg ggctgcagtg ctgacgcgat ctcagactgc
     2401 gcgacatcgt tcgtaatcgg catgtcggtc gatttgaaaa tgacgaccag ttgcgatgcg
     2461 ctgccggaca actcccgctc gactgtttgt cgcgccctgg ccgcctcggt ccggtcggag
     2521 gaaaaccctc caaccttgag cggttcatcg accctgagca acatcgggat cgatgccatg
     2581 aaggcgattg cccagatcgc aacaaccagg aaccggtgac gatacatggc tccgccgagc
     2641 ctgcggaacc gggagccacc ctgtgcggga gaacgcgtgg cagacatcag tgacgggacc
     2701 tgagggtgag caagctgaaa cgtgcggcgt cattcattcc gcgatggggt cgccgatcct
     2761 gacaaaggta ccgtctgggc cacgctcgac ggcgcgcacc gctcgcacag cattggtgtt
     2821 gagtggaccg taaatatgtg ggtatatccg ctgcggatcc tcatagtgac tgtcg
//
LOCUS       DASSGX010000156         4603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_247312, whole genome shotgun sequence.
ACCESSION   DASSGX010000156 DASSGX010000000
VERSION     DASSGX010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247312"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..568)
                     /locus_tag="VFI12_02785"
     CDS             complement(143..568)
                     /locus_tag="VFI12_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055358.1"
                     /translation="MADRWLELLDSTWTRWLEALDAVDPARRDEKVLDDNWSVKDLVA
                     HTLFWDAESLTDIHRWRSGLPGVTNDWQGMNDENHAAHKDRPYDLLRVEMHMMHEVVR
                     NTIADLPDELADELIERIADDTWDHYDDHTQQIRAWLAT"
     gene            complement(561..1007)
                     /locus_tag="VFI12_02790"
     CDS             complement(561..1007)
                     /locus_tag="VFI12_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET7055359.1"
                     /translation="MSNDQYARQYADRVAAGLATIAQVDPERRTEPGMVGHWSLKDVL
                     GHLAYWDGVHAEELESEFRGDQIIEDGRDDDIINAEQFATRESWSWDQVMDEAIANRD
                     RLVELLKRPSRYDQSGSGEHWDEHRQEIDEWLKRNQPIAAADQPNG"
     gene            complement(1004..1468)
                     /locus_tag="VFI12_02795"
     CDS             complement(1004..1468)
                     /locus_tag="VFI12_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055360.1"
                     /translation="MIATKQSPNTLSGILDRIDATWRDWMLALEAVDPARREESGVCG
                     YWSVKDIMAHIALWENEIEEHLQRWALGLPKADVDVDAMNAAVVAENKDRPYPLVRID
                     MHRAHHAAITAIRGITSELDDDVRDRIACETWDHFPEHTEQIRQWTSKESNS"
     gene            complement(1483..2343)
                     /locus_tag="VFI12_02800"
     CDS             complement(1483..2343)
                     /locus_tag="VFI12_02800"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HET7055361.1"
                     /translation="MVAEQRGSAQYEASYQAVLDAVALVPASDRSTPGVCGDWSLKDL
                     LGHLAFWDGDLATELEAKRDGDRLPTSSEREWQAINTEQAAIRADREWDDILREVRAN
                     YDRLAPLLIDPGENLDDEPIHEHWDEHFAQIEAWIADKGHAQLPITPERARDRYEDLR
                     RSALETAEGVPASLRDEPGVCGAWSLKDLMGHLAFWDSIVADRLRAKKAGHSGQLDTR
                     SFDVINAEAAAVRDDLSWDEIQHELAETHRRLLPLLTDPGEVDDYKIYMHWREHGAQI
                     EAWAARHSHI"
     gene            complement(2474..3880)
                     /locus_tag="VFI12_02805"
     CDS             complement(2474..3880)
                     /locus_tag="VFI12_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HET7055362.1"
                     /translation="MIDDGRQAIIDIAYLIAAVFFIFGLKFLSSPRQARRGNQLAAIG
                     MGLAILATFLSRDWESGNYILIIVGILIGGIAAGWAARVVKMTAMPQMTAIYNGMGGA
                     TAALVSLGEFSHKTNIGNGEKVAIVVGCIIGSISFTGSIIAFAKLQELLPGKPIRIAN
                     QQMYNIIAAVALVVLTVLAVGLDHNTTSKLILWVVFVIALALGFSVVLPIGGADMPVV
                     IAILNSLTGLAAAVAGLALGNQAMVIAGALVGASGSYLTYLMAKAMNRSVANVIFGSW
                     GAHATGGVTGGPTEQLPIRETTADDVAVQLAYADKVIIVPGYGLAVAQAQHAVRELAD
                     VLQERGVDVKYAIHPVAGRMPGHMNVLLAEANVPYEDLYEMEAINDDFPTTNVALVIG
                     ANDVTNPAARDDKTSPIYGMPILNVDEAQNVVVLKRSMKSGYAGIENMLFHDPKTSML
                     FGDAKTSLTDLINAVKAA"
     gene            complement(3877..4185)
                     /locus_tag="VFI12_02810"
     CDS             complement(3877..4185)
                     /locus_tag="VFI12_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HET7055363.1"
                     /translation="MDHSVLLGIYVFLLAIFLGIELIGRVPATLHTPLMSGTNAIHGI
                     VLLGGMVVLGEADVWWLQLIGFLAVVLGAANVFGAFVVTDRMLEMFKGRKPTAGADKS
                     "
     gene            complement(4196..>4603)
                     /locus_tag="VFI12_02815"
     CDS             complement(4196..>4603)
                     /locus_tag="VFI12_02815"
                     /inference="COORDINATES: protein motif:HMM:NF013430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HET7055364.1"
                     /translation="AELARRAARSDAIITTAAVPGRPAPRLISAETVEQMAPGSVIVD
                     LAAETGGNCELTQPGKVIDHNGVTIIGTLNLPSTLPIHASQMYAKNIQNLLALMLDKD
                     GALILNMDDDIVAGTVITYNGQVLHEGTRARMG"
BASE COUNT          772 a         1468 c         1478 g          885 t
ORIGIN      
        1 cgatgcgatg gtgtgtgtcg gcgtcgactg cgcgcgctcg accacactgc ctgggtcggc
       61 cgatcgcagg gccgccagca gcaggacgat tacgccgagg acgaagaggg cccgtttcat
      121 cgggcgatca taacgtggtc agttacgtgg cgagccaggc tcgaatctgc tgcgtgtgat
      181 cgtcgtagtg gtcccaggta tcgtccgcaa ttcgctcgat cagctcgtcg gcaagctcgt
      241 cggggaggtc ggcgatcgtg ttccgcacta cctcgtgcat catgtgcatc tccacgcgca
      301 gaaggtcgta gggccggtcc ttgtgcgccg cgtggttctc gtcgttcatg ccttgccagt
      361 cgttggtgac tcccggcagg ccgcttcgcc agcggtggat gtcggtcagc gattccgcgt
      421 cccagaagag ggtgtgggcg acgagatcct tgactgacca gttgtcgtcg agcaccttct
      481 catcgcggcg ggcggggtcg acggcgtcga gggcttcgag ccaacgggtc caggtggagt
      541 cgaggagctc cagccagcga tcagccattg ggctgatccg cggccgcaat cggctggttg
      601 cgcttgagcc actcgtctat ttcctggcga tgctcgtccc agtgttcccc gctgccgctt
      661 tgatcgtagc gagacggtcg tttcaggagt tcgaccagcc gatcccggtt ggcgatcgct
      721 tcatccataa cttgatccca cgaccacgat tcgcgagtgg cgaattgttc ggcattaatg
      781 atgtcatcgt cgcggccatc ctcgatgatc tggtcgccac ggaactcgga ttccagctcc
      841 tcggcatgca cgccgtccca gtaggcgagg tggcccagga cgtccttcag cgaccagtgg
      901 ccgaccatgc ccggttcggt gcgtcgctcc ggatcgacct gcgcgatcgt cgccaacccg
      961 gccgcgactc ggtccgcata ttgccgtgcg tattgatcat tgctcacgaa ttcgactcct
     1021 tgctggtcca ttgcctgatc tgctccgtgt gctcgggaaa gtggtcccac gtctcgcagg
     1081 cgatccggtc ccgaacgtcg tcatccagct cactggttat gccgcggatg gccgtgatcg
     1141 ccgcgtgatg tgcccggtgc atgtcgatcc ggaccagtgg atatggccga tccttattct
     1201 cggcgacgac cgctgcgttc atagcgtcga cgtccacatc cgccttcggc aggccaagcg
     1261 cccaccgctg caaatgctcc tcgatctcgt tctcccagag cgcgatgtgg gccatgatgt
     1321 ccttcaccga ccaatacccg cagacgcccg attcctcgcg gcgcgcggga tcgacggcct
     1381 cgagggcgag catccagtcg cgccaggtag cgtcgatgcg gtccaggatc ccggaaaggg
     1441 tattcggact ctgcttcgta gcgatcattg cacgctccct gctcagatgt ggctgtgacg
     1501 cgcggcccag gcctcgatct gcgcgccgtg ctcccgccag tgcatgtaga tcttgtagtc
     1561 gtcgacctcg ccgggatcgg tcaggagggg gagaaggcgg cgatgggtct cggccaattc
     1621 gtgttggatc tcatcccagg acaagtcgtc gcggacggcg gccgcttcgg cattgatcac
     1681 gtcgaaactg cgggtatcga gctgaccgga atgtccggcc ttcttcgcgc ggaggcgatc
     1741 ggcaacgatg ctgtcccaga aggcgagatg gcccatcaaa tccttgagcg accatgcgcc
     1801 acagacgcca ggctcgtcgc gcaggctggc cggcacacct tcggccgtct cgagggccga
     1861 acgacgaaga tcctcatacc gatccctggc acgctccggc gtgatgggca gctgcgcgtg
     1921 acccttgtcg gcaatccacg cctcgatctg cgcgaaatgc tcgtcccaat gttcgtggat
     1981 cggttcgtcg tccagattct cgcccggatc gatcagcagc ggcgcgaggc ggtcgtaatt
     2041 ggcccggacc tcgcgcagga tgtcgtccca ttcccggtcg gcccggatgg ctgcctgttc
     2101 cgtgttgatc gcctgccact cgcgctcgct ggaggtgggc agccggtccc cgtctcgttt
     2161 ggcctcgagc tcggtggcaa ggtcgccgtc ccagaaggca aggtggccaa gcagatcctt
     2221 cagcgaccag tcgccgcaga caccgggtgt ggaccggtcc gaagccggga cgagggcgac
     2281 tgcgtcgagc accgcctgat atgacgcctc gtactgtgcc gaaccgcgct gttctgccac
     2341 catctcgcct ctccttgact tgtaaccact agtccggcaa gaactggaat atacttgagt
     2401 tgccgctgaa acacaaacgg gttccggcag tctcccggaa cccgtttgat cgatggcgtg
     2461 ttgcggctac ggtctaggca gccttgaccg cgttgatcag gtcagtcagc gaggtcttcg
     2521 cgtcgccgaa gagcatcgac gtcttcgggt cgtggaagag catgttctcg atgcccgcat
     2581 agccggactt catcgagcgc ttgagcacga ccacattttg ggcctcgtcg acattcagga
     2641 tcggcatccc gtagatcggg ctcgtcttgt cgtcgcgcgc ggccgggttg gtcacgtcgt
     2701 tcgcgccgat gacgagggcc acgttcgtgg tcgggaagtc gtcgttgatc gcctccatct
     2761 cgtagaggtc ctcgtacggg acgttggctt cggccagcaa cacgttcatg tgccccggca
     2821 tgcggccggc caccggatga atcgcgtact tgacgtccac gccgcgctcc tggaggacgt
     2881 cggccagctc gcggaccgcg tgctgggcct gtgccaccgc cagaccgtaa ccgggcacga
     2941 tgatgacctt gtcggcatag gcgagctgga cggcgacgtc gtcggccgtc gtctcccgga
     3001 tgggaagctg ttcggtcggt ccgccggtga caccgccggt cgcatgggcg ccccatgagc
     3061 cgaatatgac gttcgcgacc gagcggttca tcgccttggc catcaggtag gtgaggtagg
     3121 aaccggatgc gccgaccagg gcgccggcaa tgaccatcgc ctggttgccg agcgcaagac
     3181 cggcaaccgc cgccgccagc ccagtgagcg agttgagaat cgcgatcacg accggcatgt
     3241 ccgcgccgcc gatcggcagc acgaccgaga agccgagggc caatgcgatc acgaagacga
     3301 cccagaggat cagcttggac gtggtgttgt ggtcgagtcc gaccgccagc accgtcagca
     3361 ccacgagggc gaccgcggcg atgatgttgt acatctgctg attggcgatg cggatcggct
     3421 tgcccggcag cagctcttgc agcttggcaa aggcgatgat cgagcccgtg aaggagattg
     3481 agccgatgat gcagccgacc acgatcgcga ccttctcgcc gttgccgatg ttcgtcttgt
     3541 gcgagaactc gccgagcgag acgagggccg ccgtcgcgcc gcccatgccg ttgtagatcg
     3601 cggtcatctg cggcatggcc gtcatcttga cgacgcgcgc cgcccaaccg gccgcgatac
     3661 cgccgatcag gatgccgacg atgatcagga tgtagttgcc actctcccag tcacggctca
     3721 ggaatgtcgc caggatcgca agacccatgc caatcgccgc gagctggttg ccgcgccgcg
     3781 cctgcctggg cgagctgagg aacttgaggc cgaagatgaa gaagacagcc gcaatcagat
     3841 aggcgatatc gataatcgcc tgtcgaccat cgtcgatcat gacttgtcag cccccgcggt
     3901 tggcttgcgg cccttgaaca tttccagcat gcgatcggtg accacgaagg ctccgaaaac
     3961 gttggcggcg cccagcacca cggccaggaa gccgatcaac tgcagccacc agacatccgc
     4021 ctcgccgagc acgaccattc cgcccaggag cacgatgccg tggatggcgt tcgtgccgga
     4081 catgagcggc gtgtgcagcg tggccgggac ccgcccgatc aactcgatgc cgaggaagat
     4141 cgcgagcagg aagacataga ttccgagcag aacgctgtga tccatcccct aacccctatc
     4201 ccatccgggc ccgggtgccc tcgtggagca cttggccgtt gtacgtgatg accgtgccgg
     4261 ccacgatgtc gtcgtccatg ttcagaatca gtgcgccgtc cttgtcgagc atcagggcga
     4321 gcaggttctg gatgttcttg gcgtacatct ggctcgcgtg gatcggcagc gtgctcggca
     4381 gattgagcgt gccgatgatc gtgacaccgt tgtggtcgat gaccttgccc ggctgcgtca
     4441 gctcacagtt gccgcccgtt tcggcggcca ggtcgacgat cacggagccc ggtgccatct
     4501 gctcgactgt ctcggccgag atcaagcgcg gcgccggccg gccggggacc gcggcagtcg
     4561 taatgatcgc gtcggatcgg gccgcgcggc gggccagctc ggc
//
LOCUS       DASSGX010000157         2279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1554335, whole genome shotgun sequence.
ACCESSION   DASSGX010000157 DASSGX010000000
VERSION     DASSGX010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1554335"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(288..1184)
                     /locus_tag="VFI12_02820"
     CDS             complement(288..1184)
                     /locus_tag="VFI12_02820"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HET7055365.1"
                     /translation="MRRRDLLIGSVALPLIRVAGGSAQSAATPVPEDQWAHPEWFADP
                     AWLAGHLDNPTVRVIALTSPEEFTKGHIPGGAQVDWPDLNLTDSADATVEKWRAAVEK
                     RLTNFSVTPESTVVIYDGGTFYASRLWWILDQLSHKDKRIIDGGLGAWTKSGGKTETV
                     STRTGNSPASPYRGTPNEATLARLAEVQQASETGSAMLVDARTPDEYGKGHIPGAVNI
                     PFLDNTVPNSGGTWKSPADLRAMYADKGVTPDRPVIPYCSTGVRSAATYFTLTALGYA
                     NVKLYSASYAEWSSDPKRPVER"
     gene            complement(1197..>2279)
                     /gene="gltX"
                     /locus_tag="VFI12_02825"
     CDS             complement(1197..>2279)
                     /gene="gltX"
                     /locus_tag="VFI12_02825"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HET7055366.1"
                     /translation="CRNLPQDEIQHSLDQGMPYTVRFAMPREGETIVNDLIRGEMVFQ
                     NALMEDLILLKSDGFPTYHLGNVVDDHDMEITHIMRGDEWIATAPIHVQLYKAFGWDV
                     PQIAHMPLILAPGGGKLSKRHGSTAMEEFRSQGYLPEALMNYLVLLGWSFDATTEIFS
                     KEDLLKDFTLERVSPSPATFDYAKLLWFNQYYINHIVSVEDLVQRLAPFLAGAGLIEP
                     DWAEPDNPKYPLLREATALLKDRIKTLAEAPELMSYFLVDELPPYDPALLVPKKTEPA
                     QIRTALQAVADLLATTDLTDLEATEAALRGLAEQQGLKAGQLFMPIRVAVTGRTESPG
                     LFETLKGIGIERVRPRIARAIALLGE"
BASE COUNT          345 a          708 c          811 g          415 t
ORIGIN      
        1 ccggtcgcgg cggtttccat tgcccgtaac cctgtacggg cttgcagcca cacgcccgca
       61 tccatcgaat tacccattgg ctgacgccgg atcggtaggg ccgtgcgggg cacggccgcg
      121 cctccccggc caccgcggtt cgcgtctggg acggcgcaca tccgccggtc ctgccctatc
      181 gatcccgggt ctggagaacc gcaacggatg tgcccccgca catccctaca gcggcgggtg
      241 ccagtcaacg ggcgatagtg cccgggcggg tttgctgtcg caaaccccta cctttcaacg
      301 ggccgcttgg ggtcgctgct ccattcggcg taggaggcgg agtagagctt gacgttggcg
      361 tagccgaggg ccgtgagggt gaaatacgtg gcggcggagc ggacgccggt gctgcagtac
      421 gggatgactg gccggtcggg cgtgacgccc ttgtcggcat acattgcgcg gaggtcggcg
      481 ggggacttcc atgtgccgcc gctgttcgga acggtgttgt cgaggaaggg gatgttgacc
      541 gcgcccggga tgtgcccctt gccgtactcg tcgggcgtgc gggcgtcgac gagcatcgcc
      601 gagccggtct cggatgcctg ctggacctca gccagccggg ccagtgtcgc ctcgttcggc
      661 gtgccacggt atggagacgc cggcgaattt cctgtccggg tcgagacggt ctccgttttg
      721 ccaccggact tggtccaggc gccgagaccg ccgtcgatga tccgcttgtc cttgtggctg
      781 agctggtcga ggatccacca gaggcgggac gcgtaaaagg tgccgccgtc gtagatgacg
      841 acggtgcttt cgggcgtgac gctgaagttg gtcagtcgct tctcgacggc ggcgcgccac
      901 ttctcgacgg tcgcgtcggc ggaatcggtc agattgaggt ccggccagtc gacctgggcg
      961 ccgccgggga tgtggccttt cgtgaattcc tccggtgagg tcagggcgat gacccggacg
     1021 gttggattat cgagatggcc ggcaagccag gcagggtcgg cgaaccactc ggggtgcgcc
     1081 cattggtcct cgggcaccgg cgtcgcggcc gattgcgcgg agccgccggc tacgcgaatc
     1141 agcggcagtg ccaccgagcc aatcagcaaa tcccgacggc gcatggcgtc ctccgatcac
     1201 tcgccgagaa gcgcgatcgc cctcgcgatg cgcgggcgga cgcgctcgat ccctatcccc
     1261 ttcaacgtct caaagaggcc cggggattcc gtgcggccgg tgaccgcgac ccggatcggc
     1321 atgaagagct ggccggcctt gagcccctgc tgctcggcca ggccgcgcag ggccgcctcg
     1381 gtggcttcga ggtcggtgag gtcggtcgtc gcgagcaggt cagcgacggc ctgaagcgca
     1441 gtccggatct gggccggttc ggtcttcttc gggacgagca gggccgggtc gtagggcggc
     1501 aactcgtcga cgaggaagta gctcatcagc tccggcgcct cggcaagcgt cttgatccgg
     1561 tccttgagca gtgcggtcgc ctcacggagc agcgggtact tcgggttgtc cggctcggcc
     1621 cagtccggct cgatcaggcc ggccccggcc aggaagggtg cgagtcgctg gaccaggtcc
     1681 tccaccgaca cgatgtggtt gatgtagtac tggttgaacc agagcagctt ggcgtagtcg
     1741 aacgtggccg gggaggggct gacccgctcg agcgtgaagt ctttgagcag atcttccttc
     1801 gagaagatct cggtcgtcgc gtcgaagctc cagccgagca ggacgaggta gttcatcagc
     1861 gcttcgggca gatagccctg gctgcggaac tcctccatcg ccgtcgagcc gtggcgcttc
     1921 gagagcttgc caccgccggg agcgaggatc agcggcatat gcgcgatctg cgggacatcc
     1981 cagccgaacg ccttgtagag ctggacgtga atcggcgcgg tcgcgatcca ttcgtcgcca
     2041 cgcatgatgt gcgtgatctc catgtcgtgg tcgtcgacca cgttgccgag gtggtaggtc
     2101 gggaagccgt ccgacttgag caggatcagg tcctccatca gcgcgttctg gaagaccatc
     2161 tcgccgcgaa tcaggtcgtt gacgatcgtt tcgccctcgc gtggcatggc gaaacggacc
     2221 gtgtacggca tgccctggtc gagcgaatgc tggatctcgt cctgcggcag gttgcggca
//
LOCUS       DASSGX010000158         4986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_682158, whole genome shotgun sequence.
ACCESSION   DASSGX010000158 DASSGX010000000
VERSION     DASSGX010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4986
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682158"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VFI12_02830"
     CDS             <1..498
                     /locus_tag="VFI12_02830"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7055367.1"
                     /translation="NLFGRVDEMPEAEWDRQIAVNLTSVFLVCKYAIPEMRARGGGAI
                     VNNASVQAFASQRTVAAYAATKGAIVSLTTVMALDHASDGIRVNCIAPGSIRTPLLEH
                     SAQTFAPDDPDAAIEQWGHDHPIGRVGTPEDVANLVLFLASDQASFCTGACYRIDGGL
                     LSALM"
     gene            753..3020
                     /locus_tag="VFI12_02835"
     CDS             753..3020
                     /locus_tag="VFI12_02835"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET7055368.1"
                     /translation="MHIFMAFLVIAVALVNPNGGGTGTAAAVQARAGAKRSVIVVMNP
                     QRSETAAAVAASVNVRPSHTYSAVFKGFAAELPEPAIRALERNPNVAFIGDDGIAAVS
                     DQEIPTGLKRIGGTSNPTAAIDGFDKNIDVDIAILDTGVDPGHPDLNVAGGKACVTGN
                     SSWADDHGHGTKVAGVAAARDNGMGVVGVAPGARIWSVKVADANGSARWSDMICGLDW
                     VAANAGTIDVANMSIAGPGSDGGCNSSGLQKSVCAVVAAGVPLVVAAGNSGQDASAYV
                     PAAFDQVITVGAIVDTDGLPGGKGSGTGSGPDDSVASFSNFGADVDISAPGYNVLTTT
                     RGGGTGGGSGTSFASPYVAGAAALYRVTHPGASPDQVRQGLIAAAAAQRGPLGFSGDR
                     DGMAEPLLWLGGDQTDSTATPTPTATRTPTPTRTPTSIPSATPTMTPTSTATPVVTAP
                     PTESIPPIDMQSPTPPAGEDIVEPTDTPQPTATVAPTEIPVPGVTLSKQKSKYNGRVM
                     ITVTGFAPMNWVTVRWQDGSVVASVATDATGAGSAWFRTPLVPYGTYRVSASDAAGAS
                     ANTRLSVISRVKNTAPVATTTGSTLRVYLYGYRPGERVELRWSGESGTTYIVLRTVTI
                     ASNGRASTLVTLPAGVSSGTFRIVGKVIGVHRSASVAFTFTIAAAQALASPTPTATAT
                     ATPIATVSPTVEPTPSPEATMTAVPPATPTVIPTDTPEPTETPTPAIDATATAPPGTP
                     EISGEGAETLPSPTP"
     gene            complement(3035..3568)
                     /locus_tag="VFI12_02840"
     CDS             complement(3035..3568)
                     /locus_tag="VFI12_02840"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET7055369.1"
                     /translation="MDRSGLLELLRGLRQTRSYSPEPVDAGTLEAILEVARWTGSGGN
                     KQPWRLVVVRDAGTRQEIAGLKADTGWVANAPVVIGIVTTGKTAESARFDAGRLVERT
                     MLAAEASGLAAAVIGFGAADSEPAMAARQLLNVPEGSWITHGVVIGHPGPRDHDAGSK
                     RGGRKPLDEIVVRERFP"
     gene            complement(3791..4714)
                     /locus_tag="VFI12_02845"
     CDS             complement(3791..4714)
                     /locus_tag="VFI12_02845"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7055370.1"
                     /translation="MSATTATLADVDVAQPARAMRKSQGLVRRALGTFVRDRVAMTAL
                     VIVILLVIFALGADVVSHLTGFSAQENHLTEKLSKPGENGYILGSDANGRDILTRLAY
                     GGRISMTVAFLATLFELTIGLGIGLLAGYAGKWVDFILMRLVDVLLSIPTLPLLILIS
                     TLYRPGTYLLALILALVSWPGDSRLLRGEALSLKSREYVDAARVVGVSPFGIVVRYIL
                     PNVMPTMLVLASLTVPSLIIAETSLSFLGVGVQVPTPSWGNMLDEAYRFYRTNWTNVF
                     IPGFIVFAASLALYLVGDGLRDALDPRRSSR"
     gene            complement(4711..>4986)
                     /locus_tag="VFI12_02850"
     CDS             complement(4711..>4986)
                     /locus_tag="VFI12_02850"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7055371.1"
                     /translation="VRVLYGHAFRNALLTLTTLIGLSSPGLFGGALLIEYIFAWPGMG
                     SLTIEAVGRRDYTMVQATTLLFAFLTMIGNMLADLIYGLIDPRVRVS"
BASE COUNT          902 a         1798 c         1533 g          753 t
ORIGIN      
        1 aacctcttcg gccgcgtcga cgagatgccc gaggccgagt gggatcgcca gattgctgtc
       61 aacctcacca gcgtcttcct cgtctgcaag tacgcgatcc ccgagatgcg agcgcggggc
      121 ggcggcgcga tcgtcaacaa cgcctcggtt caggccttcg cctcgcaacg gaccgtcgcg
      181 gcctacgcgg ccaccaaggg cgcgatcgtc tcgctgacca ccgtcatggc gctcgaccac
      241 gcatcggacg gcatccgggt caactgcatc gccccgggct cgatccggac gccgctactc
      301 gaacactcgg cccagacctt cgcgcccgac gatccggacg ccgcgatcga acagtggggc
      361 cacgatcacc cgatcggccg cgtcggcacc cccgaggacg tcgcgaacct tgtcctcttc
      421 ctcgccagcg accaagcctc cttctgcacc ggcgcgtgct accgcatcga cggcggcctc
      481 ctctcagccc tgatgtaacg gcgttcgttc cctcccaagc gcgtgcggtg aggtcagccc
      541 gccgggacca aagatggata tccggccgtg acctgcgtcc catcctgatc cccgtaccgg
      601 ttgcgggtgg tcatctgtac tgccgtccca tgcccattcg gtagcccagc gccgaagatc
      661 gaggcatgaa tccatgccca gatcgaccac acgttcacgc aggtccggcg acacagcgat
      721 cggcccgtca gcggcagtcg cgccgtgcgg tcatgcacat attcatggcc ttcctggtga
      781 tcgccgtcgc cttggtcaac ccaaacggtg gcggcacggg gactgcggct gcggttcagg
      841 cccgggccgg cgccaagcga agcgtcatcg tcgtcatgaa cccgcaacgc agtgagacag
      901 cggctgccgt tgccgcctcg gtcaatgtgc gcccgtccca tacctactcg gcggtgttta
      961 agggcttcgc ggccgagctc cccgagccgg caatccgcgc actcgagcgg aatcccaatg
     1021 tggcattcat cggcgacgat gggatcgccg ccgtctctga tcaggagatc ccgaccggtc
     1081 tcaagcgaat cggcggaacg tcgaacccca cggccgcaat cgatggcttc gacaagaata
     1141 tcgacgttga tatcgccatc ctcgacaccg gcgtcgatcc cggccatcca gacctcaatg
     1201 ttgctggcgg aaaggcctgc gtgaccggca acagctcctg ggccgatgac catgggcacg
     1261 gcacgaaggt cgctggggta gcggccgcgc gagacaacgg catgggcgtc gtcggcgttg
     1321 cacccggtgc ccggatctgg tcggtgaagg tggccgatgc gaatggcagc gcccgctggt
     1381 ccgacatgat ctgcggcctt gattgggtcg ccgcgaatgc gggcaccatc gacgtcgcca
     1441 acatgagcat tgccggtccc gggagtgacg gagggtgcaa ttcctccggg cttcagaagt
     1501 cggtctgcgc cgtcgttgcc gccggcgtgc cccttgttgt ggccgcgggt aattcggggc
     1561 aagacgcgtc ggcctatgtg ccggccgcat ttgaccaggt catcaccgtc ggcgcgatcg
     1621 tcgacaccga cgggcttccg ggcggcaagg gcagcgggac cgggagcggt cccgacgatt
     1681 cggtcgcttc cttcagcaac ttcggcgcgg acgtcgatat ctccgcaccc ggctacaacg
     1741 tgctgaccac cacgcgcggc gggggcaccg gaggcggatc gggcacgagc ttcgcctccc
     1801 cgtacgttgc cggcgcagcg gcgctctacc gcgtaactca cccaggcgca tcccccgatc
     1861 aggttcggca aggcctgatt gcggcagcgg cagcgcaacg cggaccgctc ggcttctccg
     1921 gcgatcgcga cgggatggcc gagcccctgc tctggctcgg cggcgaccaa accgacagca
     1981 ccgcaacgcc caccccgacc gcgactcgaa cccccacccc gacccggaca ccgaccagca
     2041 ttccctcggc gacgcccacg atgactccaa caagcaccgc gacaccggtt gtgaccgccc
     2101 cgccgacgga gagtattccg ccgatcgaca tgcagtcgcc cactccccct gccggggaag
     2161 acattgtcga gccgaccgat accccgcagc cgaccgcgac ggtggcgccg accgagattc
     2221 ctgtcccggg agtcacactc tccaagcaga agagcaagta caacgggcga gtcatgatca
     2281 cggtcaccgg ctttgcgccg atgaactggg tgaccgtgag gtggcaagac ggcagcgtcg
     2341 tcgcatcggt tgcaacggac gcgaccggcg ccggcagtgc ctggttcagg acgccgctcg
     2401 ttccgtacgg cacgtaccgc gtgtcggcaa gcgatgcggc cggcgccagc gcgaacacca
     2461 ggctttcggt catctcccgc gtcaagaaca ccgctccagt cgccacgacc actggatcga
     2521 cattgcgggt ctacctctac ggctatcgcc ccggggagcg cgtcgagctg cgctggagtg
     2581 gggaatcggg cacgacgtat atcgtgctga gaaccgtaac catcgccagc aatggccggg
     2641 catcgacact cgtgaccctg ccggcaggcg tctccagcgg tacgttccgg atcgtcggca
     2701 aggtaatcgg cgtccaccga agcgcgtcag tggcgttcac gttcacaatc gctgccgccc
     2761 aggcgctggc cagccccacg ccgaccgcaa cggcgaccgc cacgcccatt gcgactgtgt
     2821 cgccgacggt cgagccaaca ccctcacccg aagccaccat gacagccgtg cctccggcca
     2881 ccccgactgt gatcccaacg gatacgccgg agccgaccga gacaccgacg ccggccatcg
     2941 acgcgaccgc gactgcccct ccgggtacgc cggaaatcag cggagaagga gccgagacac
     3001 tcccgtcacc aactccgtaa gacacaggtg gtatctacgg gaagcgctcc cgcaccacga
     3061 tctcgtcgag cggcttgcgg ccaccgcgct tgctgcccgc atcgtgatcg cgcgggccgg
     3121 ggtggccgat tacgacaccg tgtgtgatcc acgacccctc ggggacgttc agcaactgcc
     3181 gggccgccat cgcgggttcg gagtcggccg cgccgaaccc gatcaccgcc gccgccaacc
     3241 cgctcgcctc tgccgccagc atggtccgct cgaccaatcg cccggcatcg aaccgggccg
     3301 attccgctgt tttgcctgta gtcacgatcc cgatcacgac gggcgcattc gccacccacc
     3361 cggtatccgc cttcagcccg gcaatctcct gccgcgtccc ggcatcgcgg actaccacca
     3421 accgccacgg ctgcttgttg ccgccgctcc cggtccaccg cgccacctcg agaatcgcct
     3481 cgagcgtccc cgcatccacg ggctccgggg aatacgatcg cgtctgccgc aacccccgca
     3541 acaactcaag caagccagat cgatccatat tcgcctcctg ctggtgaggt atcgaaaatc
     3601 gtgtggtaat ggaattcgta gcgcggggtt accatgcccc gctgtggccg caggccacga
     3661 tagtcctgcc gaggacccgg tccgcgcatc ggaaccggcg tggtcggaaa cgatcatcgc
     3721 cctgtgggcg attgcggggc atggtaactc cgccctacgg ttcggtatcc ggggttaggc
     3781 gacgttctgc ctagcgcgag ctccgcctcg ggtcgagcgc gtcccgcagc ccgtccccga
     3841 ccaggtacag cgccagcgat gccgcgaaga cgatgaaacc cgggatgaag acgttcgtcc
     3901 agttcgtccg gtagaaccgg tacgcctcat ccagcatatt cccccagctc ggcgtcggca
     3961 cctgcacccc gacaccaagg aagctgagcg acgtctcggc gatgatcaac gagggcaccg
     4021 tcagcgaggc gagcaccagc atcgtcggca tcacgttcgg caggatatac ctgacgacga
     4081 tcccgaacgg cgagaccccg accactcgcg ccgcatcgac gtactcgcgc gatttcagcg
     4141 acaacgcctc gccccggagc aaccgggaat cgcccggcca cgaaacgagc gccaggatca
     4201 gcgccagcag gtacgtcccc ggccggtaaa gcgtgctgat caggatcagc agcggcagcg
     4261 tcgggatcga gagcaacacg tcgaccagcc gcatcaggat gaaatcgacc cacttgccgg
     4321 cataccccgc cagcaggccg atgccgagac cgatcgtcag ctcgaacagc gtcgccagga
     4381 acgcgaccgt catcgagatc cgcccgccgt acgccaagcg ggtcagaatg tcccggccgt
     4441 tggcgtcgct gccgaggatg tagccgttct cgcctggttt cgagagcttc tcggtcaggt
     4501 ggttctcctg cgcgctgaac ccggtcagat gcgaaaccac gtccgccccg agcgcgaaga
     4561 tgaccagcag gatcacgatc accagcgcgg tcatcgcgac ccgatcgcgc acgaacgtcc
     4621 caagcgcgcg ccggaccagc ccctgcgact tgcgcatcgc ccgggccggc tgggcaacat
     4681 cgacgtcggc gagggtggcg gtcgtcgcgc tcatgagacg cgcacccgcg ggtcgatcag
     4741 gccgtagata aggtcagcca gcatattgcc aatcatcgtc aggaaggcga agagcagtgt
     4801 cgtcgcctgg accatagtgt agtcccgccg cccaactgcc tcgatcgtca gcgagcccat
     4861 ccccggccag gcgaagatgt actcgatcag cagcgcgccg ccgaacagcc ccggactcga
     4921 gaggccgatc agcgtggtca gcgtcagcag cgcgttccgg aaggcgtggc cgtagagcac
     4981 ccgcac
//
LOCUS       DASSGX010000159         2661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1336533, whole genome shotgun sequence.
ACCESSION   DASSGX010000159 DASSGX010000000
VERSION     DASSGX010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1336533"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..1251)
                     /gene="pheS"
                     /locus_tag="VFI12_02855"
     CDS             complement(166..1251)
                     /gene="pheS"
                     /locus_tag="VFI12_02855"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HET7055372.1"
                     /translation="MSESPPVLDRLKAIETDAARALERDVADLGSLADWERSWLGSSG
                     AITGILRSMGQLPAELRPEVGRTANQVKQRLTVAYDERKGALHAGALSNRLADETIDV
                     TLPGRTPLVGTVHPISQMIDEVSEIFAYLGFETVVGPEVEIGKYNFDMLNIPADHPAR
                     DVWDTIIVDDDRDEIVLRTHTSPMQARVMEQRTPPIRVIVPGRVYRFEQQDASHEWMF
                     HQIEGLALDDRITMADLKGTLTELARQLFGEDTRVRFRCDFFPFVEPGVEFAVSCPFC
                     GGKGCRVCKRSGWIEMGGAGMVHPNVLRAVNYDPDTVQGWAFGLGVERLVMVKYGVND
                     IRAFAGNDLRFLRQFARADIGLGVASR"
     gene            1616..>2661
                     /gene="eno"
                     /locus_tag="VFI12_02860"
     CDS             1616..>2661
                     /gene="eno"
                     /locus_tag="VFI12_02860"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HET7055373.1"
                     /translation="MITSIRAREILDSRGNPTVEVDVELAEGVVGRAAVPSGASTGAY
                     EAVELRDGDKRRYGGKGVLTAVGNVNDEIAQEVVGMSALDQRAIDTVMVQLDGTVNKG
                     RLGANAILGVSLAVARAAAQWSGLPLFKYLGGPNASTMPVPMMNILNGGKHALGSSVD
                     MQEFMVMPIGAASFREGLRMGTEVFHALKDILHDRGLGTGVGDEGGFAPSLESNEAAI
                     QVVTEAIKKAGYRAGEDLVIALDPASTELFHGGRYELKGEGRTLTSSEMVDYWTEWAR
                     KYPIKSIEDGLAEDDWDGWKSLTEQVGSEVQLVGDDLFVTNTERLSRGIREHAGNSIL
                     VKLNQIGTLTETFDT"
BASE COUNT          488 a          853 c          867 g          453 t
ORIGIN      
        1 gcttcgagac ctcgttcact tcggcgacga agaccttgtc gccccacatc tcgccgattt
       61 gcgtgatctt ctccgcctcg agaccggcca tcgtcagacg atgggccagt tcttgaggcg
      121 cgaggccgga ctgcacgaac tcggcgagcc acttcatagg aacgttcatc tactcgccac
      181 tcccaatccg atgtccgccc gcgcgaattg ccgcaggaag cgcaaatcgt tccccgcgaa
      241 ggcgcgaatg tcattcacgc cgtacttgac catcaccaat cgttcgactc cgaggccgaa
      301 cgcccaaccc tgcaccgtgt ccgggtcgta gttgaccgcg cggaggacgt tcgggtggac
      361 catgccggcg ccgcccatct cgatccagcc agatcgcttg cagacccggc aaccctttcc
      421 gccacagaac ggacaggaga ccgcgaactc aacaccgggc tcgacgaacg ggaagaagtc
      481 gcaccggaag cggacgcggg tgtcctcgcc gaaaagctgc cgggcgagct cggtgagggt
      541 gcccttcagg tcggccatcg tgatccgatc gtcgagcgcg aggccctcga tctggtggaa
      601 catccactcg tgcgacgcgt cctgctgctc gaagcgatag acgcgtccgg gcacgatcac
      661 gcgaatcggc ggcgtgcgct gctccatcac ccgcgcctgc atcggcgacg tgtgggtgcg
      721 caggacgatc tcgtcgcggt cgtcgtcgac gatgatcgtg tcccagacat cccgcgccgg
      781 atggtcggcc gggatgttga gcatgtcgaa gttgtacttg ccgatctcga cttccggccc
      841 aacgacggtt tcgaaaccga ggtaggcgaa aatctcgcta acctcatcga tcatctgcga
      901 gatcgggtgg accgtgccga ccagcggcgt ccggccggga agcgtaacgt cgatcgtctc
      961 atctgccagc cggttgctga gcgcgcccgc gtgcagcgcg cctttccgct cgtcgtaggc
     1021 cacggtcagg cgctgcttga cctggtttgc tgtccgccca acttcgggac gaagctcggc
     1081 tgggagctga cccatgctcc gcaggatccc cgtgatcgct cctgacgagc cgagccagga
     1141 gcgctcccag tcggcgaggg acccgaggtc cgcgacatcg cgctcgagcg cccgagctgc
     1201 gtccgtttcg atcgctttca gccggtcgag gaccggcggt gattccgaca ttgctgcaca
     1261 tccgctcatt ttccgcgcat acaaaaaccc ctcggcaggg acgaggggtc gccacgcggt
     1321 accaccctgt ttcaaccgga ccattcggtc cagctgcgct tgacatgccc gataacggag
     1381 gctccggccc gactacgcaa cgatcacgtc ttcaccaggc tgctcgggaa ggaattcgcc
     1441 ggtccggggc cggttccgct tccagcgtgc tcgcggaact ctctgctcgg tcgggtccgg
     1501 gtactggttt ccctcaacgc gcacatcaat tgtgcgccaa gtataacggg tcaacctcct
     1561 atactcagcc gggtaactga gcaggttcga agagagggac aagcgcgtgt cggcgatcat
     1621 taccagcatc cgggcccggg agattcttga ttcgcggggc aaccccacgg tcgaggtcga
     1681 cgtcgagttg gccgagggcg tggtcggccg cgctgccgtg ccctccggcg catcgaccgg
     1741 cgcatacgag gcggtggagc tgcgcgatgg cgacaagcga cgctatggcg gcaagggcgt
     1801 cctgaccgcg gtcggcaacg tcaacgacga gattgcccag gaagtggtcg ggatgagcgc
     1861 gctcgaccag cgcgccatcg acacggtgat ggtccaactg gatggcacgg tgaacaaggg
     1921 ccgcctcggc gcgaacgcga tcctgggagt ctcgttggca gtggcgcggg ctgccgcgca
     1981 gtggtcggga ttgccgctct tcaagtacct cggcgggccc aacgctagca ccatgccggt
     2041 gccgatgatg aacatcctga acggcggcaa gcacgcgctc ggctccagcg tcgacatgca
     2101 ggagttcatg gtcatgccga tcggcgcggc ctcatttcgc gaagggctgc ggatgggcac
     2161 ggaggtcttc cacgcgctca aggacatcct gcacgaccgg ggattgggta ccggtgtcgg
     2221 cgacgagggc ggattcgcgc catcgctcga gtcgaacgag gcggcaatcc aggtggtgac
     2281 tgaggcgatc aagaaggccg gctatcgcgc aggagaggac ctggtgattg cgctcgatcc
     2341 ggccagcacc gagctgttcc acggcggaag gtacgagctc aagggcgagg gccggaccct
     2401 caccagcagc gagatggtcg attactggac tgaatgggcg cggaagtacc cgatcaaatc
     2461 gatcgaggac ggcctggccg aggacgactg ggacggctgg aagtcgctga ctgagcaggt
     2521 aggctcggag gtccagctcg tcggtgacga cctgttcgtg accaacaccg agcggttgag
     2581 ccggggcatc cgggagcatg ccggcaactc gatcctggtc aagcttaacc agatcggcac
     2641 gctgaccgaa accttcgaca c
//
LOCUS       DASSGX010000160         2703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_791993, whole genome shotgun sequence.
ACCESSION   DASSGX010000160 DASSGX010000000
VERSION     DASSGX010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791993"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="VFI12_02865"
     CDS             complement(<1..855)
                     /locus_tag="VFI12_02865"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET7055374.1"
                     /translation="MWWHNGTPEQDHLNAYWDAAVGGATPEAPAQGDPGLDPTMQEAV
                     AQVRALHRRHRPDPAFALQLESVLMNAFTTTYPKATVIQPVRPKRLVTRPAWLPGREE
                     IFPRRWGLAALATVALVAITLAVFFFNLRDTHQPAVVPGTPVATPTSTPAPATVEPVS
                     MYRGNPARTGVLPGPSLTGAPVELWKLQTGEPVNLGAAVVDGVLYLSAGSQGFEARDA
                     MTGDVIWTFPTDASAVSAPAVVDGQGYVIDTDGTLYAVRTSDGTETWRMANINVRSSV
                     APVDGLVYA"
     gene            complement(842..1429)
                     /locus_tag="VFI12_02870"
     CDS             complement(842..1429)
                     /locus_tag="VFI12_02870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7055375.1"
                     /translation="MSEHRGSHASDARLVASAKRGDAEVFALLYRRYLDRVYDFVAAR
                     LEGVEAAQDATQTIFLRALSALPQCRDEEHFAGWLFAIARNVVIDTYRSPRQVLVEFD
                     AILEIEDRAESPESLVMRSEEIHALREARDRCLSGVERELLDLRLQDLTSQEIAVAMK
                     RSQTAIRNVQYRMIKKLRDCLGIRATQGEETHVVA"
     gene            complement(1732..2556)
                     /locus_tag="VFI12_02875"
     CDS             complement(1732..2556)
                     /locus_tag="VFI12_02875"
                     /inference="COORDINATES: protein motif:HMM:NF024231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET7055376.1"
                     /translation="MGAIRSNWMIGLASLIGLAVYLYPFALPTIRQTDSGAARAGEAP
                     IVLCGLVLLCLAAILREIDPMRAGEGAARIVALLASMVALGAVARLIPSFLGASPIFL
                     LVILGGYVFGPAFGFQLGALTLAVSAFLTGGIGPWVPYQMLGVGWVGMLAGVLPKPVR
                     RGRQLGVLAVYGVVSGVLFGALLNLWFWPFAAPGGGEVAPGLSWSPDLSLAEALGRYI
                     RFYVITSLWFDVFRAIGNVVLILALGRPILRTLERYRDRFTWRPWTPGTDAMPSET"
BASE COUNT          502 a          901 c          889 g          411 t
ORIGIN      
        1 cgcatagacg aggccatcga ccggcgccac cgaggagcgg acattgatgt tggccatccg
       61 ccaggtctca gtgccatcgc tggtccgcac ggcgtagagc gtgccatccg tatcgatgac
      121 atacccttgg ccatcgacca ctgccggcgc ggaaacggcg gacgcgtcag tcgggaaggt
      181 ccagatcacg tcaccggtca tggcgtcgcg ggcctcgaag ccctgcgatc cggcggacag
      241 gtacagcacg ccgtcgacca ctgcggcccc gagattgacc ggttccccgg tctggagttt
      301 ccagagctca acgggtgcgc cagtcaggct gggaccgggg aggacgccgg tgcgggccgg
      361 gttgccgcgg tacatgctga ccggctcgac cgttgccgga gccggtgtcg atgtcggcgt
      421 ggcgacgggg gtgcctggca cgactgcggg ctggtgcgtg tcccggagat tgaagaagaa
      481 gaccgcgagg gtgatggcga cgagtgccac cgtggcgagc gcggccaggc cccagcggcg
      541 cgggaagatc tcctcccgcc ccggtagcca cgcgggccgg gtcacgagtc gcttcggtcg
      601 cactggttga atgacagttg ctttcggata ggtcgtagta aaggcgttca tcagaacact
      661 ctccaattgg agggcgaacg ccggatcagg ccggtgcctc ctatggagcg cccggacctg
      721 ggcgacagcc tcctgcatcg tcggatcaag accgggatcc ccctgggccg gcgcttccgg
      781 agtcgcgccg ccgactgcgg catcccagta cgcattcagg tggtcctgct ccggcgttcc
      841 gttatgccac cacatgggtt tcctcccctt gcgtggcccg gatgcccagg cagtcccgga
      901 gctttttgat cattcggtac tgcacattgc ggatcgcggt ttggctccgc ttcatcgcaa
      961 cggcgatttc ctgactggtc agatcctgca agcggaggtc gagcagctcg cgctcaaccc
     1021 ccgacaggca ccggtcccgc gcctcccgca aggcgtggat ctcttccgag cgcatcacga
     1081 gcgattccgg cgattcggcg cgatcctcga tctcgaggat cgcatcgaac tccaccaaaa
     1141 cctggcgcgg agaccgatac gtgtcgatga cgacattgcg cgcgatcgca aacagccagc
     1201 ccgcgaagtg ctcttcgtcg cggcattggg ggagggcgct caacgcgcgc aggaagatcg
     1261 tctgcgttgc atcctgcgcg gcttcgacac cctccaaccg agcagcgacg aagtcgtaga
     1321 cccgatccag gtagcggcgg tacagaagcg cgaagacttc cgcatcaccc cgtttggccg
     1381 aggcgaccag gcgagcgtcg cttgcgtgcg atccgcggtg ttcgctcaca ttcaccctga
     1441 tgggctccgc agcgagcccg gtgctgatta cgtctgagag ccatggcaaa cgcgcgttca
     1501 cctatagaga cggttccccc ggcaaaacgt cgcaccgaag ccggggtctc gaccggtcag
     1561 gggtgaaaca gccgtccgcc gttctgatcg cgacgacgaa tcaggctcga ctgccaacgt
     1621 tgaagatgtg aggtttccgt gtcaaatata tcgcaggtgt gaaccccgaa tccggcacga
     1681 tgcattcgaa cgggcgtggg gttcaaccga gcggcccacg gggggaccgc gctacgtttc
     1741 gctgggcatg gcatccgtgc ccggggtcca cgggcgccag gtgaagcggt cgcggtagcg
     1801 ctcgagggtg cgcaggatcg gccggcccag ggcgaggatc aggaccacgt tgccgatcgc
     1861 gcggaagaca tcgaaccaga gcgaggtgat gacgtagaag cggatgtacc ggcccagcgc
     1921 ctcggccagg ctcaggtcgg gcgaccacga tagccccggc gcgacttccc cgccgcctgg
     1981 cgccgcaaac ggccagaacc agaggttgag cagcgccccg aacaagaccc cgctgaccac
     2041 gccatagacc gcgagcaccc cgagctgccg cccgcgccgg accggcttcg gcaacacccc
     2101 ggccagcatc ccgacccagc cgacgccgag catctggtac ggcacccacg ggccgatccc
     2161 gccggtcagg aaggcggaga cggcgagcgt caacgcgccg agctggaatc cgaaggccgg
     2221 cccgaagaca tagccaccga ggatcaccag caggaagatc ggcgacgcgc cgaggaatga
     2281 cgggatcagc cgggcgaccg ccccgagcgc gaccatcgag gccagcaagg cgacgatccg
     2341 ggctgccccc tcgccggcgc gcatcgggtc gatctcgcgc aggatcgcgg ccaggcagag
     2401 caagaccagc ccgcagagga cgatcggcgc ctccccggcc cgcgccgcgc ccgaatcggt
     2461 ctgccggatc gtcggcaggg cgaaggggta gagatagacc gccagcccga tcaggctggc
     2521 cagcccaatc atccaattcg accgaatcgc gcccaacggc gcatcttgtc ccggctcaat
     2581 cattgtgcaa gactgccaaa caggacttca gacgtcagac ttcggacttc ggttttggct
     2641 gatggcggcc aggggttgga tccttggtcc atcaactatc taccaaccac tatcaaccgt
     2701 ctg
//
LOCUS       DASSGX010000161         2621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_138928, whole genome shotgun sequence.
ACCESSION   DASSGX010000161 DASSGX010000000
VERSION     DASSGX010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138928"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="VFI12_02880"
     CDS             complement(<1..673)
                     /locus_tag="VFI12_02880"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7055377.1"
                     /translation="MSLFRANFGVSRLFCASSPHRIHNQAVRCRDETDSPMPMTVLVV
                     EDDHEIAQLVRDYLERAGFNTTWAGDGDEALLQVRNGRPDLIVLDLGLPKRDGLDVIR
                     EVRAHAQTPIVILTARGEETDRIVGLELGADDYIVKPFSPREVVARVRAVLRRATAPE
                     ADDRILRRGSMEIDLPRMRVTVDGRTVELTATEFQLLATMARRPGQVFTRGQLLDAVR
                     GDAFEG"
     gene            802..1542
                     /locus_tag="VFI12_02885"
     CDS             802..1542
                     /locus_tag="VFI12_02885"
                     /inference="COORDINATES: protein motif:HMM:NF039734.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6345 domain-containing protein"
                     /protein_id="HET7055378.1"
                     /translation="MRFSTGGVDNYAPCSSTSLSFCRQNGRDFRTWYQFSGHTAVTNW
                     EDGDVWGSDFRDGASADGDPSGGSELPQIYFYAGHGTCQAAPTATDPDFISVCGNFGA
                     PNRTNIGQTTRWGNNAGSLNFAFLDASCPMDLVSIGANWFNVFLGLHVATGHSGTRTQ
                     DTLDSADRASQFAALTAGLPWPFPTMSIGDAWMQTGIIDIQPGCCAVAIANGRDIPEA
                     DRRVDFEQVNSGWSDPVGDWFVWKWVCR"
     gene            1559..2377
                     /locus_tag="VFI12_02890"
     CDS             1559..2377
                     /locus_tag="VFI12_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055379.1"
                     /translation="MSIYDQREHNDDDTHRLQAVGAEILGLPLGEIEREGTTANMTGI
                     QSSRYQLTSRTDSRTIFLQDSEFQAQGGVGVFEGNDDELLERAARILSNLGIDEREID
                     QRSVLTEQIEAASFDRRTGAVASAGVRDGKRFALLTRAIDGIPIWQSSVTLGLTRDGS
                     PGYLHLHWPEIERKVLDMARGYLSAESSGWSPPTIDYAEPESWRAGVLHSPAISLVLD
                     QIAAIKVIYRPLTDEIGKKPVRFVDLDGRDVPLPRHFEETPGPVDEKRPGANAA"
BASE COUNT          498 a          868 c          791 g          464 t
ORIGIN      
        1 atccctcgaa cgcatcgcca cgcacggcat cgagcagctg cccgcgcgtg aaaacctgcc
       61 ccggacgccg cgccatcgtc gccaggagct gaaactcggt tgcggtcagc tcgaccgtgc
      121 gcccgtccac ggtgacgcgc atgcgcggca ggtcgatttc catactgcca cgccgaagaa
      181 tccgatcatc cgcttccggc gcggtcgcgc ggcgcaagac cgcccgcacc cgcgccacca
      241 cctcacgcgg gctgaacggc ttgacgatgt agtcatccgc tcccaattcc aacccgacga
      301 tgcggtcggt ctcctcccca cgggcagtca ggatcacgat cggggtttgc gcatgcgcgc
      361 gcacctcccg aatcacatcg aggccatcac gcttcggcaa tcccaggtcg agcacgatca
      421 gatcgggccg cccgttgcgc acctgcaaga gggcctcatc gccatccccg gcccacgtcg
      481 tgttgaaacc ggcccgctcc agatagtcgc gcaccaattg agcaatctca tggtcatctt
      541 cgacaactaa aactgtcatt ggcatcggcg agtccgtttc atctctgcat cgtaccgcct
      601 ggttgtgaat gcgatgtgga gaggaggcgc agaacaaacg ggaaacgccg aaattcgcgc
      661 ggaaaagcga catttcatgc ccaaactgtg ggtattgcgt gtgttcaaca ccggcagcgc
      721 atcctagcct gtgagcaccg gaatggttcc ggaagaggtg tacgccgatc tcgatccgat
      781 ctcacctagg gagtacacac gatgcgattc tcgaccgggg gcgtggacaa ctacgctcca
      841 tgcagctcga ccagtctcag tttttgccgg cagaacggcc gcgacttccg cacctggtac
      901 cagttcagtg gccacaccgc ggtgaccaac tgggaggacg gcgatgtctg gggctcggac
      961 tttcgcgacg gcgcttcggc cgacggcgac ccgtccggcg gcagtgagtt gccgcagatc
     1021 tatttctatg ccggtcacgg cacctgccag gcggcgccca cggcgaccga tcctgacttc
     1081 atctcggtct gcggcaattt cggcgcgccc aaccgcacca atatcgggca gacgacccgc
     1141 tggggcaaca atgccggcag tctgaatttc gcctttctgg atgcgagctg tccgatggat
     1201 ctcgtttcga tcggcgccaa ctggttcaat gtctttctcg gtctgcatgt cgccaccggg
     1261 cactccggca cgcgaacgca ggacacgctt gacagcgccg accgggccag ccagtttgcc
     1321 gcgctcacgg ccgggctgcc ctggccgttc ccaacgatga gcatcggcga tgcctggatg
     1381 caaaccggca tcatcgatat ccagcccgga tgttgcgcgg tcgccatcgc caacggccgg
     1441 gatatccccg aggccgaccg ccgggtcgat ttcgaacagg tcaacagtgg ctggtccgat
     1501 ccggtcggag attggttcgt ctggaaatgg gtttgccgct aagcaacgga ggttcccaat
     1561 gtcgatctac gatcaacgcg aacacaacga cgacgatacc caccggctgc aggcggttgg
     1621 cgcagagatt ctcggtctcc cactgggcga gatcgagcgc gaaggcacga cagcgaatat
     1681 gaccggcatc caatccagcc ggtaccagct cacgtcgcgc accgattccc gcacgatttt
     1741 cttgcaagac agtgaattcc aggcacaggg cggcgtgggc gtgttcgagg gcaacgacga
     1801 cgagctcctc gagcgggcag ctcggatcct gagcaatctc ggtatcgacg aacgcgagat
     1861 cgaccagcgc tcggtgctca ccgagcagat cgaagcggct tcgttcgacc ggcgcaccgg
     1921 cgcggtcgcc tcggcgggcg tgcgggacgg caagcggttc gcgctgttga cgcgtgcaat
     1981 cgatggcatc ccaatttggc aatcgagcgt cacgctcggg ctcacgcgtg acgggtcccc
     2041 ggggtatctg caccttcact ggccggagat cgagcgcaaa gtgctcgaca tggcccgcgg
     2101 atatctgtcc gcggaatcaa gcggctggag tccgccgacc atcgactatg ccgagccgga
     2161 atcctggcga gccggcgtgc tgcattcccc ggcgatctcc ctggtgctcg accagattgc
     2221 ggcgatcaag gtgatctacc gcccgctgac cgatgagatc ggcaagaaac cggtccgttt
     2281 cgtcgatctg gatgggaggg atgttccctt gccgcgccac ttcgaggaaa cgcccggtcc
     2341 agtcgacgaa aaacgcccgg gcgcgaacgc ggcctgatcc cgtcattcct gacccggccg
     2401 gatcgcgtgt ccgatccggc cggcaattga cgttctgcac accggtcatc catgattgca
     2461 gcaccggtcg atccaaatac ccgcttccgg agccagccgt ggggcaaacg gcgccagcag
     2521 tcgagttcga acttcccatt ccacgaacac cgctgatcgg ccgcgagcgc caactggcgg
     2581 aaattcgcgg gttgctcctc cgggatgatg tgcccctcgt g
//
LOCUS       DASSGX010000162         3940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_247969, whole genome shotgun sequence.
ACCESSION   DASSGX010000162 DASSGX010000000
VERSION     DASSGX010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247969"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="VFI12_02895"
     CDS             complement(<1..459)
                     /locus_tag="VFI12_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7055380.1"
                     /translation="MHSAAKAWFRLAGLAVTLALVLPLVAITAHAQDTAKVLRIHHQV
                     YPDVVDPQKSGFVNEIDILALAYEGLTRIDDQQQTVPGAAESWEYNDDATQITFHLRD
                     GLKYSDGSPLTAENFRYAVERTCDPKTAGEYQYLLFEVVGCADFAGLNADA"
     gene            complement(630..2312)
                     /locus_tag="VFI12_02900"
     CDS             complement(630..2312)
                     /locus_tag="VFI12_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7055381.1"
                     /translation="MNQLFRHWARLAALAAVFALIVPVMTTGTAAQDGGKVLRVHQIT
                     YPDVVDPQKSSFTSEIAILALAYEGLTRLDTSQQTVPGAAESWEYNDDATQITFHLRD
                     GLKYSDGSPLTAENFRYAVERTCDPNTAGEYQSILFEIAGCEDFAGLALDADGNPREF
                     TQEEYDAAKAKLGAKALDETTLQLDLTHPAPYYHTIASLWVFYPVKQEIATKDPDNWW
                     KTAENHIGNGPFKVTGIAEDQQWTFEANDNYWQGRPKLDAIEYHYVEDSQVALEAYRT
                     GDLDTVDLEPPQVAEVQADPDLSQAFVTYPTASTYNLGMNLTQEPFNDPKVREAFSYA
                     VDRETLCNVIRSGDCTPTLSWIPAGIPGAIETDKFGFDPDKAKQALAESSYGSADKLP
                     EIKLYYNSDVSGRGDQVEWVAGQFRDILGVEVSIEPTDGTTLTALRKDSTTFPQLLFV
                     GGWIQDYPDAQNWLSVYWTCASTFSERVGYCNEDFDKLVSQGDTTIDPAERVKYYEQA
                     GQILVDDVPGPFLYNESALFVVNPAVTGYTPTASEVEWPGYLSSIMTIDKTG"
     gene            complement(2526..3737)
                     /locus_tag="VFI12_02905"
     CDS             complement(2526..3737)
                     /locus_tag="VFI12_02905"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HET7055382.1"
                     /translation="MRVGLPVRAAWRSLRSYRLFAAIGLLLLAGWGALPFGASAQEWS
                     APRTVYIPTTGHTSDGLFLDVWRAEPDLLGDPVTEEFRARTDFNTSGDAAADVIQYYE
                     HLALIYLPHESAEKQVQALDLGRQALAEATTAGRSVALQRALERSLCSPAAGSDCASV
                     VATGHTLRDAFLAYWQAGDAATWLGLPLTESLRAPDGTRVQYFENAILRQTADGSVAP
                     LPLGNVAARRAKIETKAIEQPQDVPVYDEELFIPPAPATPVDDAGGGDDYWTLGPGPQ
                     QGAWKEVVVSISNEKMWAYEGGELVMSSLVSTGTAETPEVTTPTGFFSVLVKYDSQTM
                     EGTISGQHYRVEDVPYVMYFDNRGDALHGTYWHNNFGHPMSHGCVNLPMDVAAWMYDW
                     APVGTAVSVIW"
BASE COUNT          663 a         1304 c         1282 g          691 t
ORIGIN      
        1 tgcgtcggca ttgagcccag cgaagtccgc gcagccgacc acttcgaaga gcagatactg
       61 atattcaccg gcggtcttcg ggtcgcaggt gcgctcgacc gcatagcgga aattctccgc
      121 cgtcaagggc gagccatcgc tgtatttgag accgtcccgc agatggaagg taatctgggt
      181 ggcgtcgtcg ttgtactccc acgactccgc cgcgcccggc acggtctgtt gctgatcgtc
      241 gatgcgagtc agcccctcgt aggcgagagc cagaatgtcg atctcgttga cgaagccgct
      301 tttctgcggg tcgacgacgt cggggtagac ctggtggtgg atgcgcagca ccttcgcggt
      361 gtcctgcgca tgcgcggtga tggcgaccaa cggcaggacg agcgcgagcg tgacggccag
      421 tcccgcgagc cggaaccatg ctttcgccgc tgaatgcacg ggtgcctcct tgccatgtgt
      481 cagatgcccg gaccgtccgc actgccgcgg agcaacgtga accctaccgg agcccgaaag
      541 gcgagtcaat cctgacgcaa aacgtgggcc ggcgcacgca gcagggcgcc ccggattccg
      601 gggcgccctg tgtcatgccg tgcggttgac tatcccgtct tgtcgatcgt catgatcgag
      661 gagaggtagc ccggccactc gacctcgctc gcggtcgggg tgtacccggt gacggccgga
      721 ttgaccacga acagcgcgga ctcgttgtag aggaacgggc ccggcacgtc gtcgaccaga
      781 atctggccag cctgctcgta gtacttgacc cgctccgcgg ggtcgatcgt ggtgtcgccc
      841 tggctgacga gcttgtcgaa gtcctcgttg cagtagccga ctcgctcgga gaaggtcgag
      901 gcgcaggtcc agtagacgct cagccagttc tgcgcgtccg gatagtcctg aatccagccg
      961 ccgacgaaca gaagctgcgg gaacgtcgtg gaatccttgc gcagcgcggt cagcgtggtg
     1021 ccgtccgtgg gctcaatgga gacttcgacg ccaaggatgt cgcggaactg cccggcgacc
     1081 cattcgacct gatcgccacg gcccgagacg tcgctgttgt agtagagctt gatctctggc
     1141 agcttgtccg cgctcccata cgacgactcg gcaagcgcct gcttggcctt gtctgggtcg
     1201 aagccgaact tgtcggtctc gatcgcgccg gggatccccg ccgggatcca ggagagcgtc
     1261 ggcgtgcagt cgccggagcg aatcacattg caaagcgtct cgcgatcgac cgcatacgag
     1321 aatgcttcgc gcaccttggg gtcgttgaac ggctcctgcg tcagattcat cccgaggtta
     1381 tacgttgagg ccgtcgggta ggtgacgaac gcctgggaga ggtcggggtc cgcctgcacc
     1441 tcagcgacct gcggcggctc caggtcgacg gtatcgagat caccggtgcg atatgcctca
     1501 agcgccacct gggagtcctc gacatagtgg tattcgatgg cgtccagctt gggccggccc
     1561 tgccagtagt tatcgttggc ctcgaaggtc cactgctggt cctcggcgat cccggtcacc
     1621 ttgaacgggc cgttgccgat gtggttctcg gccgtcttcc accagttgtc gggatccttg
     1681 gtcgcgatct cctgtttgac cgggtagaac acccacaggg acgcgatggt gtggtagtag
     1741 ggcgcggggt gggtcaggtc gagttgaagc gtcgtttcat cgagcgcctt cgccccgagc
     1801 ttcgccttcg ccgcgtcgta ctcctcctgt gtgaactccc gcgggttgcc gtcggcatcg
     1861 agcgccaagc cagcaaaatc ctcgcagccc gcgatctcga agaggatcga ctggtattcg
     1921 cccgccgtgt tgggatcgca ggtgcgctcg accgcgtagc ggaaattctc cgccgtcaac
     1981 ggcgagccgt cgctgtactt gaggccatcg cgcagatgga aggtgatctg ggtggcgtcg
     2041 tcgttgtact cccacgactc ggctgcgccc ggcaccgtct gctggctcgt gtcgagacgg
     2101 gtcagcccct cataggccag ggccagaatg gcgatctcgc tcgtgaacga actcttctgc
     2161 ggatcgacga catcggggta ggtgatttga tggacccgca ggactttgcc gccgtcctgc
     2221 gccgccgtcc cagtcgtcat gaccggcacg atgagggcga aaaccgcggc gagcgcggcc
     2281 agtctcgccc aatggcggaa tagctggttc atgggcaccc tcctgtcgtc atcgtcgctc
     2341 cccggtcgca tccttgcccc gggcgtttgg ggaaccctac cgaacgttgc cccgcccgtc
     2401 aatcggcaaa atggggacgt ggaacacgca aacggcccgg gctcctgccg tggcagcccg
     2461 gcaccgtcaa acgcgaagaa gcccggcgcg ggagccgggc ttcacgtcag gtccaggcga
     2521 ccggtttacc aaatgacgct gactgcggtt cccaccggcg cccagtcgta catccaggcg
     2581 gcgacgtcca tcggcaaatt gacgcagccg tggctcatgg ggtggccgaa gttgttgtgc
     2641 cagtaggtgc cgtgcagggc gtcgccgcga ttgtcgaaat acatgacgta cggcacgtcc
     2701 tcgacccggt agtgctgacc gctgatcgtc ccctccatgg tctgcgagtc gtatttgacg
     2761 aggacggaga agaacccggt cggcgtcgtc acctccggcg tctcggccgt cccggtgctg
     2821 accagggagg acatcaccag ctcgccgcct tcgtacgccc acatcttctc gttggagatg
     2881 gagacgacga cctctttcca ggcgccctgc tgcggaccgg ggccgagcgt ccagtagtcg
     2941 tccccgccgc cagcgtcatc gaccggcgtg gccggcgccg gcgggatgaa gagctcctcg
     3001 tcgtagactg ggacgtcctg cggctgctcg atcgctttcg tctcgatctt ggcgcgccgg
     3061 gcggcgacgt tccccagcgg cagcggcgct acgctcccgt ccgccgtctg ccggaggatc
     3121 gcgttctcga aatactggac ccgcgtccca tccggggccc gcaacgactc cgtcaatggc
     3181 aacccgagcc aggtcgcggc gtcgccagcc tgccagtagg cgaggaacgc gtcgcgcagc
     3241 gtgtggccgg tagccaccac gctcgcgcaa tcactaccgg ccgcgggcga gcagagcgac
     3301 cgctccagcg cgcgctggag cgcgaccgag cggccggcgg tggtcgcctc ggccagcgcc
     3361 tgccgaccga gatccagcgc ctggacctgc ttctccgccg actcatgcgg caggtagatc
     3421 agcgccagat gctcgtaata ctggatgaca tcggccgcgg cgtcgccact ggtattgaaa
     3481 tcggtacggg cacggaactc ctctgtgacc ggatcgccga gcagatcggg ctcagcgcgc
     3541 cagacatcga ggaacagccc gtccgatgtg tgacccgtgg tgggaatata gactgtgcgc
     3601 ggcgccgacc actcctgggc cgacgcgccg aatgggagcg ccccccaccc ggcgagaagc
     3661 agcagaccaa tcgccgcgaa caaccggtac gagcgcaggc tccgccaagc cgcgcgaacc
     3721 ggtagaccga ccctcattgc gaacccccag gaacacccac acgcgccgcg cggacgcggc
     3781 gcgccgaaaa cctctcccca ccaacagtat actcctctgc aagtgatgta cgtacgcgat
     3841 gtgaccaggc agacaacccc cggcgatcgc gccgcggccc cggaatggct ttcgccagcg
     3901 ccgctcgcgg acgatttacc gcgcctccac gccgatccgc
//
LOCUS       DASSGX010000163         5068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_357188, whole genome shotgun sequence.
ACCESSION   DASSGX010000163 DASSGX010000000
VERSION     DASSGX010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5068
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357188"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1303
                     /locus_tag="VFI12_02910"
     CDS             <1..1303
                     /locus_tag="VFI12_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055383.1"
                     /translation="GWHIQFSRIAFPNIMWPLTVLIGAIALNEAVRSGSRAWWALAGA
                     GFAFGIYTYNGDFLYLGIVGLAMAWILFGWSAALAAVLIGGAYVSPSGFALVRVAIGL
                     GLLAMNERVRTRRQLRAAASFAIGFGVVVWPMAKFILGNQETYFGRGKGVTIFRTEQW
                     TTLGSLNEKASFLARRYVDFWDTLTRHPVLDGVAGNGSHGVVPVTAVVLAAVGIAFAV
                     ARRMHPIVLLGTLTVLLAPLATVASNDFALRRSMIVAPFLAMFGGIAVTEIIRISWNR
                     RVVVRVAAMTIVLALSGNIGYRNVHDYFTDTARSQSTRWVMGPEIVATARYLDTMPAG
                     AYVYFFSMRWPFTYETIRYFAPDAHGETRGAPYGPNAIDIDPANGAAVFVLLDQYQAR
                     LPEIQERYPGGETVVGPRLTNPDTGPTYIAYVLPTQQATAP"
     gene            complement(1335..1736)
                     /locus_tag="VFI12_02915"
     CDS             complement(1335..1736)
                     /locus_tag="VFI12_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055384.1"
                     /translation="MAPGLSIVQVLVENNVNPATGGDAPDHLEIALRNDSTAELRGFV
                     VYYEITDLTTGAKEGYCSALTGFTIPAGGERTAHFDNSGAADHFPDNEFSLYHQSLNE
                     MQVDVTVSATGVAPQSASVTKEAGGAENPDE"
     gene            1747..2100
                     /locus_tag="VFI12_02920"
     CDS             1747..2100
                     /locus_tag="VFI12_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055385.1"
                     /translation="MIGFEVIGKMSPSAEATHSAGISLAATGVQLGAGEATGEPDVEG
                     VAIAEPDAAGEAAGVEHGPGVDAVVCMTDEKAATPPLAATAAILATNSTTTTASILRI
                     RAITRLLLSGGRLID"
     gene            complement(2060..3046)
                     /locus_tag="VFI12_02925"
     CDS             complement(2060..3046)
                     /locus_tag="VFI12_02925"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7055386.1"
                     /translation="MSNTALMTLAGHDYRSAARNRVLGALLVTLLVVTTVSVLIAAFD
                     YQTKIDAYRQYKDAAIAAGIQNVAPLQVRPLQLMRPVVEYIEFVGAVIAIALGYLSVA
                     RERANRTVWLLRTRPVSSSALTFGPFLGAIGLISTLVVATAAIGVISIGVWGHDWLNG
                     EELVRLALWCIASIVYMAIFYCLGAALTARSRVLANGLVAALVVWLAVVLIIPQIGDT
                     MDPDNQLPGGLFRSMNLDKSHETRVLAKFGTYERLRNDLEEVSFAKQYERFSFAILEI
                     KLKDTFRDTSISHVLNATRRNTEWLALYAAVMAGVLYYTVSRSTAHPKGEDA"
     gene            complement(3039..3956)
                     /locus_tag="VFI12_02930"
     CDS             complement(3039..3956)
                     /locus_tag="VFI12_02930"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET7055387.1"
                     /translation="MIALIAAHQLRMLRRERIFLAILAALLLMTALAGFIGWSSHNTI
                     VKVYRESGAMLASEGKPAPPDPFALKPHLSLLSNMTIYVMLIGALLAVIVGHLAIVSD
                     RVSGTTRLIFARPVPRSSYFLGKLAGIGAALGGILAVSYLLSVISLIIVNSSLPSAEE
                     FGRLALFYLLSLAYMLLFALIGVTAALVVPGRSLALLVALGVWMVITFMIPQFTSGLR
                     PTASLNPVVDPVATSQRFFDITAHFRSVSLSELYRDLSAAVLQTAPDNANSVGGDEII
                     PISAILVGLMLLVATLVNRLDLSEQGSHE"
     gene            complement(3953..4630)
                     /locus_tag="VFI12_02935"
     CDS             complement(3953..4630)
                     /locus_tag="VFI12_02935"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7055388.1"
                     /translation="MLRDINLVIEHGEIFGFLGHNGAGKTTTISILTTLLRPTRGEAR
                     VCGYDVVTGRLDVTRNIGYLPDSVRLYDHLTGIENLEFFGKLSGMRNPLSAARETLAI
                     LDCDGYAHLRVGSYSTGMRQRVAIAQAVMHRPRVLFLDEPTSGLDPSGVRQLRETIKR
                     MNDELGMTIFMNTHLLSEVARVCTTIGVLNHGELIYKDTIENTTMRYRDEASLEEIYL
                     RMEDGVA"
BASE COUNT          907 a         1518 c         1748 g          895 t
ORIGIN      
        1 cggatggcac atccagttct cgcggatcgc cttcccgaac atcatgtggc cgctcacggt
       61 gctaatcgga gcgatcgcgc tcaacgaggc agtgcgctcc ggctcgcgag cctggtgggc
      121 actggcgggg gccgggttcg cgttcggcat ttacacctat aacggcgact tcctctacct
      181 cggcatcgtc ggcctggcga tggcctggat cctgttcggt tggagcgccg cgctggcagc
      241 cgtcctgatc ggcggggcgt acgtctcgcc ctccgggttc gcgctggtcc gggtcgcgat
      301 cgggcttggg ttgctggcca tgaacgagcg cgtgcggacc cggcggcagt tgcgcgcggc
      361 ggcatcgttc gcgatcggct tcggcgtcgt ggtctggccg atggcgaaat tcattcttgg
      421 gaaccaggaa acgtacttcg ggcgcggcaa gggcgtgacg attttccgga ctgagcaatg
      481 gaccacgctt ggttccctca acgagaaggc atctttcctt gcacggcgat acgtcgattt
      541 ctgggacact ctgacccggc atccggtcct ggacggcgtc gccgggaacg gatcgcacgg
      601 tgtggtcccc gtcacagcgg tagtcctcgc agccgttggc atagcattcg cggtcgcgcg
      661 gcggatgcac ccgatcgttt tgctggggac tttgaccgtc ctgctggcgc cgttggctac
      721 ggtcgcctcg aacgactttg ccttgcggcg gtcgatgatc gtcgcgccgt tcctggccat
      781 gttcggcggg atcgccgtga cggagatcat ccggatctcg tggaaccggc gggtggtggt
      841 gcgggtcgcg gcaatgacga tcgtgctcgc gctgtcgggg aatatcggat accgcaatgt
      901 ccacgattac ttcacggaca cggcccggtc gcaatcgacg cgctgggtga tggggccgga
      961 gatcgtcgcg acggcgcggt acctcgacac catgccggcg ggcgcctacg tctacttctt
     1021 ctcgatgcgg tggccgttca cgtacgagac aatccggtac ttcgcgccgg acgcgcacgg
     1081 ggagacgcgg ggtgcgccgt atggaccaaa cgctatcgat atcgaccccg caaatggagc
     1141 ggcagtgttc gtgctccttg accagtatca ggcgcgcctg ccggagatcc aggagcgcta
     1201 cccgggcggc gaaacagtcg tggggccgcg cctgaccaat ccagataccg gccccacgta
     1261 catcgcctat gtgctgccga cgcagcaggc gaccgctccg tgacgcggtt gcgtgagtcc
     1321 ccaacagacg cgcactactc gtcagggttc tcggcgccgc cggcctcctt ggtgaccgag
     1381 gcggactgcg gggccacgcc agtcgcactg acggtgacgt cgacctgcat ctcattcagc
     1441 gattggtggt acaggctgaa ctcgttgtcc gggaagtgat cagccgcccc ggaattgtcg
     1501 aaatgggcgg tgcgctcgcc gccggcgggg atcgtgaagc cggtcaaggc gctgcaatag
     1561 ccttctttgg cgccggtggt cagatcggtg atctcgtagt agacgacgaa gccgcgcaat
     1621 tccgctgtgc tgtcgttgcg cagggcgatc tcgagatggt cgggcgcgtc cccgcccgtg
     1681 gccggattaa cgttgttctc gacaagcacc tgtacgatcg acaggccagg cgccaccgac
     1741 gcattcgtga tcggattcga ggttatcggc aagatgagcc cgtcggctga ggcgacacat
     1801 tcggcgggta tctcacttgc ggcaaccgga gtgcaattgg gtgcgggaga agcgaccggc
     1861 gagcccgatg tggagggggt cgcaatcgcc gagcccgacg cggcgggtga ggcggcggga
     1921 gtggagcacg gtccgggcgt agacgccgtc gtttgcatga cggacgagaa ggcggcaact
     1981 ccgccgctcg ccgcgacggc cgcgatactc gctaccaaca gcaccacgac gacggcttcg
     2041 atcctgcgca ttcgggcaat cacgcgtctt ctcctttcgg gtgggcggtt gatcgactga
     2101 ccgtgtaata gagcacgccg gccatcaccg cggcgtacag cgccagccac tcggtgtttc
     2161 tccgcgtcgc attgaggacg tggctgatcg aggtgtcgcg gaaggtgtcc ttgagcttga
     2221 tttcgaggat cgcgaacgaa aagcgctcgt actgcttggc gaaggagacc tcttcgaggt
     2281 cgttgcggag gcgctcgtac gtgccgaact tggcgagcac ccgggtttca tggctcttat
     2341 cgagattcat ggaccggaac aggccgcccg gaagctggtt gtccgggtcc atcgtgtcgc
     2401 cgatctgggg aatgatcagg accacggcca gccagacgac cagtgccgcc acgagtccat
     2461 tggcgagcac gcggctgcgg gcggtgagcg cggcgcccag gcagtagaag atggccatgt
     2521 agacgatcga ggcgatgcac cacaaggcca gccggaccaa ctcttccccg ttgagccagt
     2581 cgtgtcccca gacgccaatc gagatgacac cgatggccgc ggtcgcgacc accagcgtgg
     2641 aaatcaggcc gatcgctccg aggaagggac cgaacgtgag cgcgctggag gagaccggac
     2701 gggtgcgcag cagccagacc gtgcggttcg cgcgctcgcg agcaacgctc aggtagccga
     2761 gggcgatcgc gatcactgca ccaacaaact cgatgtattc gaccaccggc cgcatgagct
     2821 ggagcggcct gacctgcagc ggcgcgacgt tctggatacc ggcggcgatg gcagcgtcct
     2881 tgtactgccg gtaggcatcg atcttcgtct ggtagtcgaa ggccgcaatc agcaccgaaa
     2941 ccgtcgtcac gaccagcagt gttacgagga gcgcgcccag gacgcggttg cgcgcggcgg
     3001 agcgatagtc gtggccggcg agggtcatca atgcggtgtt actcatggga cccttgctcc
     3061 gagagatcga gccggttgac gagcgtcgcc accagcaaca tgaggccgac caggatcgca
     3121 ctgatcggga tgatctcgtc tccgccaacc gaattcgcat tgtccggcgc ggtctgcagg
     3181 acagcggcgc tcagatcgcg gtacagctcg ctgagcgaca ccgagcggaa atgggcagtg
     3241 atgtcgaaga agcgctgcga cgtggcgact gggtcgacca ccggattcag cgacgccgtt
     3301 gggcgcaatc ccgacgtgaa ctgcggaatc atgaaagtga tgaccatcca gacaccgagc
     3361 gcgacgagga gtgccagcga gcgaccggga acgaccagcg ccgccgtgac gccgatcagg
     3421 gcgaagagga gcatgtaggc cagcgacagg aggtagaaga gcgcgagccg gccgaattct
     3481 tccgcggagg gcagcgagct gttgacgatg atgagcgaga tgacgctcag gaggtagctg
     3541 accgccagga tgccgccgag tgccgcgccg atgccggcca gcttgccaag gaaatagctc
     3601 gaacgaggaa cgggccgggc aaagatgagg cgagtcgtgc cggacacccg atcgctgacg
     3661 atggcgaggt ggccgacgat gaccgcgagc agagcgccga ttagcatgac gtagatcgtc
     3721 atgttggaga ggagcgagag gtgcggcttc agggcgaacg ggtccggcgg cgccggcttg
     3781 ccttcgcttg cgagcatcgc gccagactcc cggtagacct tgacgatcgt gttgtggctc
     3841 gaccagccga tgaagccggc cagcgccgtc atcagcaaca gcgcggccag gatcgcgagg
     3901 aagatacgct cgcgacgcag catccggagc tggtgggcgg cgatcagcgc gatcatgcca
     3961 ccccgtcctc catgcgcaag tagatctcct caagcgatgc ctcgtcgcgg taacgcatgg
     4021 tcgtgttctc gatcgtgtcc ttgtagatca actcgccgtg attgagcacg ccgatcgtcg
     4081 tgcagacccg cgcgacctcg ctgaggaggt gcgtattcat gaagatcgtc atgccgagct
     4141 cgtcgttcat ccgcttgatg gtctcgcgga gctggcggac gccactcggg tcgagaccgg
     4201 aggtcggctc gtcgaggaag agcacgcgcg gccgatgcat gacggcctgg gcgattgcga
     4261 cccgctggcg catgccggtc gaataggatc cgacccgcag gtgggcatag ccatcgcagt
     4321 cgaggattgc gagcgtctcg cgcgccgccg agagcgggtt gcgcatgccg gagagctttc
     4381 cgaagaattc gagattctcg atgccggtga ggtggtcata aagacggacg ctatcgggca
     4441 ggtaaccgat attgcgcgtg acatcaagcc gcccggtgac aacgtcgtac ccgcagactc
     4501 gcgcctcgcc gcgtgttggc cgcagaagcg ttgtcaggat actgatagtg gtcgtcttgc
     4561 cggcgccgtt gtggccgagg aatccgaaga tctcgccgtg ctcgatcacg aggttgatgt
     4621 cgcgcagcac tcggtgggat ccgtagctgt gggagaggtc tcgaagctca atcgcgggcg
     4681 gcgtgcgccc caattctgtg gtgttcatcc aagcccccag cgaagttgct tcccgaacaa
     4741 tttcgagcag ggtcgatgca gttgtggatc gcggttggcg ggacgagaga tgcacgagtc
     4801 ccggggcgct ccaggtggcg cagctccgtc caaccacgga tccgtctgag ctattccagc
     4861 ctctggcggg ccggccttcg gcgaggcgag cgtacggtga tcacatgaac tccagatgaa
     4921 gttatggagg ccgctgttcg gggtccatca agccgttcgc gtgaggaccc agccaaagtg
     4981 tccagccggc ccgtgtgacc ctgattcacc gggtggccta cctccgttac gcggggcaca
     5041 ttggcgaagt tcgggctctt gtgggagt
//
LOCUS       DASSGX010000164         4730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_248863, whole genome shotgun sequence.
ACCESSION   DASSGX010000164 DASSGX010000000
VERSION     DASSGX010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4730
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248863"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1380)
                     /locus_tag="VFI12_02940"
     CDS             complement(<1..1380)
                     /locus_tag="VFI12_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055389.1"
                     /translation="MDALALHTARGTLPSSLTRLVGRADELLALAALVRDPEVRLLTL
                     TGPGGVGKTRLALTVARQVSDAFPDGVLFIDLSPIRDPALVAPTIAQTLGLSGGSNPV
                     QLVLSVIAQDHCVLVLDNFEQVVDAAPVVADLLIGAPDLTIIATSREPLKVSGEQEYP
                     VAPLALPPAALQSPDELAHFDAVQLFAARARAVVPSFVLAPVNAPQVADICRRLDGLP
                     LAIELAAALVKTLPVSVLLARLEQRLPVLAGTRRDSPERQQTMRNAIAWSYMLLPPDE
                     QTLFRRLGVFVGGCTLEAAEAIGAVGDTGIDVIRALSSLVDKSLVRLDSAALGGPRYL
                     MLETIHEFALEHLQDSAEHDAVRAAHAAWFSRVAEERQMHGDNWIEPPSAGHAVPPVE
                     VDFANVRAAMSWYGDSGNVTELARMAGGVYWYWHVHGPRREGLDLLRTAWHANPDTVR
                     DKQSRMWAME"
     gene            complement(1989..3998)
                     /locus_tag="VFI12_02945"
     CDS             complement(1989..3998)
                     /locus_tag="VFI12_02945"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET7055390.1"
                     /translation="MVDEERQATTHATVVSRRRLLVGATAFAVAGLSAGLGLRGPVTQ
                     AQNQGPIEPNPLGSPIPEEVTQAASDWPLVHGDYKAHRNGAGATISTENIGDIDITWR
                     APLTAAGYYGAITSPAIVLGETIFFQDGESNVFAVDRTTGDLKWEHVFSAVNFGPSGV
                     SIGYGMIYGGTGPQGEVFALNAETGEEVWRHKLSISADGATVMAPTVYDNVVYTCLDE
                     GYRGGARGFLYALDAKTGAVLWRWDTSTDNLWGAARLNGGAGIWYPASVDDAGHIYLG
                     TGNPAPFPANNGETRPGPNLYSSSMVSLDVTTGSVRWYVQAAPHDVLDHDFQQTPILA
                     TITDGDTERLLAIGAGKVGTVIAADAITGRVVWEKRVGEHNEYGDGAPLPATPVVVLP
                     GTYGGVESPMALANDTLFVPVLNLPSTFSATEWVIPEGQTLADARSELIALDVHDGSE
                     KWKVAIDTFVAAGATVANDIVFTGGLDGVVHGYNIETGEEVWTYQANGGLNAPFSVSG
                     DMLFVPVGSPIYKKSGEVQAPQTEMIAFRIGGNESGTPVVQPALPHGASPSEATPVTE
                     PAPPPAAATPEGAAGAAVTVTMVDLAFEPKAFSIPADTPTRVSLPNAGALPHNFSIDA
                     LKISVDAAPGATGEVTINAPAGTYEYYCNVPGHRDAGMVGTLTVS"
BASE COUNT          869 a         1507 c         1513 g          841 t
ORIGIN      
        1 ctccatagcc cacatccggg actgcttgtc tctcacggtg tccgggttgg cgtgccacgc
       61 cgtgcggagc agatccagcc cttcgcggcg cggcccatgc acgtgccaat accagtagac
      121 accgcccgcc atccgggcca actccgtgac attgcccgaa tcgccatacc aactcatggc
      181 ggcgcggaca ttcgcgaagt cgacctcgac gggcgggacg gcgtggccag ccgacggcgg
      241 ctcgatccag ttatcgccgt gcatctgtcg ttcctcggcc acgcgcgaga accaggcggc
      301 gtgggcggcg cggactgcgt catgctcagc gctgtcctga aggtgctcga gcgcaaactc
      361 atggatcgtt tcgagcatca ggtagcgggg tccgccaaga gccgctgaat ccaggcgcac
      421 caagctcttg tcgaccagcg atgagagggc gcgaatcacg tcgatcccgg tgtcgccgac
      481 cgcaccgatc gcttcggcgg cttcgagcgt gcagccgccg acgaagacgc cgagccggcg
      541 aaagagcgtt tgctcatccg gtggcaacag catgtagctc caggcgatgg cattgcgcat
      601 ggtctgctgg cgctcgggcg aatcgcgccg cgttccggcg agcacgggca accgctgttc
      661 gagccgggcg agaaggactg acaccgggag cgtcttgacg agcgcggcgg ccagctcgat
      721 ggccaggggc aggccatcca ggcggcgaca gatgtccgcg acctgcggcg cgttgaccgg
      781 cgccagcaca aacgatggca ccactgcccg ggcccgtgcg gcaaagagtt ggacagcgtc
      841 gaagtgggcc agctcatcag gggactggag cgccgccggt gggagggcaa gcggcgcgac
      901 cgggtattcc tgctcaccac tcaccttgag cggttcccgg ctggtcgcga tgatggtgag
      961 gtcgggcgcc ccgatcagca ggtcggccac gaccggcgcc gcatcgacga cctgctcgaa
     1021 gttgtcgagc acgagcacgc agtgatcctg ggcaatgacg gagaggacga gctgcaccgg
     1081 attcgatccc ccactcaggc ccaacgtctg ggcgattgtg ggcgccacca gggcgggatc
     1141 gcggatcggg gacaggtcaa tgaagaggac cccgtccggg aaggcatcgc ttacctgccg
     1201 ggcgaccgta agcgcaaggc gggttttgcc gaccccgccg ggaccggtca gcgtcagcag
     1261 gcggacctcg ggatcgcgca ccagcgcggc gagcgccagc agctcgtccg cccggccgac
     1321 gagtcgggtg agggacgacg gtaaggtgcc gcgtgccgtg tgaagcgcca gcgcgtccat
     1381 ggcccatctc cgccacgtga acagtcgtgt gccatgacca tgccctgaaa ccgcgcgctc
     1441 gtcaacaggg cagggtggtt tggttgaaca cggagcattc aacggacacg ccgagcttgg
     1501 caacactgtg ttcgcgatcg gtccagcgga gggttactac cggcacattg tgttcagtac
     1561 cgctcaacgt tttacaactg cgatggcgac gagatcggac agtgtttcgc cttcgtgatt
     1621 ggtgattggt tgagaactcg tatgcgcgcc tcgcgccttc cgaacggcaa atgaccaacc
     1681 cgtcaccaac ggagaaacgg tttgatcttg agttggatac catccgcatt gcaaaagcca
     1741 taactcggcg gcagcccgac ttcgagcccg ttgacgttcc agttgctatt ccccacccct
     1801 cgccgagaac gtctctgtcc cctgacgcgc gatggccacc tgaaccttcg aaggcccacc
     1861 tcgattggcc gatctgatca ataaacagcc gccgagacga gcgtctcggc ggctgttgtg
     1921 ataccgtgac cggtcggatt ggcgagatga cttcagaaaa cagcacgctc gagcagcgca
     1981 ggagactcct acgatacagt gagcgttccc accatcccgg catcccggtg gccgggtaca
     2041 ttgcagtagt actcataggt gccggctggc gcatttatcg tgacctcgcc cgtcgcaccc
     2101 ggggcggcat cgacgctgat cttgagggcg tcaatagaga agttgtgggg tagcgcaccg
     2161 gcgttgggca gcgacacccg agtgggcgtg tcggccggga tagagaaggc ctttggctcg
     2221 aaggcgaggt cgaccatcgt gaccgtcacg gccgcgcccg cggctccctc tggcgttgcg
     2281 gccgctggtg gcggcgcggg ctcagtcacc ggcgttgctt ccgacgggga ggccccatgc
     2341 ggcaatgccg gctggaccac tggcgtgccg gactcgttgc ccccgatgcg gaaggcgatc
     2401 atttcggtct ggggggcttg cacctcaccc gacttcttgt agatcgggct gcccaccggc
     2461 acgaagagca tatcgcctga gacggagaag ggcgcattga ggccgccatt ggcctggtag
     2521 gtccacacct cttcaccagt ttcgatgttg taaccatgga cgacgccatc gaggccgcca
     2581 gtaaagacga tgtcattggc caccgtcgcg ccggcagcga cgaaggtgtc aatggcgacc
     2641 ttccacttct cggacccatc gtgaacgtcg agcgcgatga gttcacttct cgcatcggcc
     2701 aatgtctgtc cctccggaat aacccattcg gtggccgaaa aggttgaggg cagattgagc
     2761 acggggacaa agagggtgtc gttggccagg gccatcggcg attcgacgcc gccgtaagtg
     2821 cccggcaaga ccacgaccgg ggtggccggc agcggcgcgc catccccata ctcgttgtgc
     2881 tccccgacgc gcttttccca gaccacgcgg ccggtaatgg catcggcggc aatgacggtc
     2941 ccaaccttgc cggcaccgat cgccaacagt cgctccgtgt cgccgtcggt gatggtggcc
     3001 aggatcggcg tctgctggaa atcatgatcg agcacgtcgt gcggcgcggc ctgcacgtac
     3061 cagcgcaccg agcctgtggt gacgtcgagc gagaccatcg agctggaata gagattcgga
     3121 cccgggcggg tctctccgtt gttggcgggg aagggtgccg ggttgccggt cccgagatag
     3181 atgtggcccg catcatccac cgaagcgggg taccagatac cggccccacc gttgagccgg
     3241 gcggcccccc agaggttgtc cgttgaggtg tcccagcgcc agaggactgc gcccgtcttg
     3301 gcatcgaggg catagagaaa gccgcgggcg ccgccgcgat agccctcgtc caagcaggtg
     3361 tagacaacat tgtcatagac cgtaggcgcc atgacggtcg ccccatcagc ggaaatggac
     3421 aacttgtgcc gccagacctc ctcgcccgtc tcggcattca gggcgaagac ctcgccttgc
     3481 gggccggtcc cgccatagat catgccgtag ccaatcgaga cgccactggg accgaagttg
     3541 acggcgctaa agacgtgctc ccacttcaga tcgccagtcg tccgatccac cgcgaatacg
     3601 ttgctttccc cgtcctggaa aaagatcgtc tcacccagga cgatcgccgg tgaggtaatc
     3661 gcgccgtagt acccagcggc cgtcaggggt gcgcgccagg tgatatcgat atcaccaatg
     3721 ttctccgtac tgatcgtggc gcccgcgcca ttgcggtggg ctttgtagtc gccgtggacc
     3781 aggggccagt cgctggccgc ctgggtgact tcctcgggaa ttggcgagcc gagcggattg
     3841 ggttcgatgg ggccctgatt ctgggcctgg gttaccgggc cccgcaaccc aagacctgcg
     3901 gacagtccgg ccacggcaaa cgccgtcgcc ccgacgagca gacggcgtct tgatacaacc
     3961 gtcgcgtgcg tcgtcgcttg gcgttcttca tcaaccatga gttcgttccc ccgtacccga
     4021 ttccgaccca ttgctcggaa tgagagattg tcactctcgg tatgaaacct gtcaacagcc
     4081 cggtacgttc ggatatctcc tttttgcgtg ggccgagatc acccgccctc aagatcgggg
     4141 gttaccgtgc tcgctgcgtc tgcttatctc ccgtagcgag ttcaccccac tccagcgcag
     4201 gtcgtgcaac ccgcacgcaa ctacttttca ggcggtcgta ccgcaccatg ggcctcgaca
     4261 ccggccctgc tcgcagaatg cgagcaggat ttcacgctgc gtgatgagaa cgatggatcc
     4321 ggattgatgt ttcagttgat atgggatccg acccgctgct ccgtcccgga gcggcgacgg
     4381 atcggggtcc gctacgtata ggctgagcac agcggaatca tctggtgcct ttcggtcggc
     4441 tgccgcgtcg aacctttggt ctctccaacc cagggtgacc agcggcacat tgtgttcagt
     4501 accgctcgcc gatgtgaacc caattgcacc gccgatcaat cccggttttg cacggaactg
     4561 ctagtcggtg gcaaaagcag ccgtttccga gtcagtccaa cgaagttgaa gggcggatcg
     4621 gttgcgattg tgttgtgaac cacaagtggt acgcctcaat ggccgtgatg aacacgctcg
     4681 gcgtcgcccg cctcaaaggc atcaaaccgg cctaacccgc caccatccac
//
LOCUS       DASSGX010000165         2535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_902947, whole genome shotgun sequence.
ACCESSION   DASSGX010000165 DASSGX010000000
VERSION     DASSGX010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_902947"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VFI12_02950"
     CDS             complement(<1..446)
                     /locus_tag="VFI12_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055391.1"
                     /translation="MPRLATYLIPDPETRLYQIASAILGYDVFAERELPLPEPLQPHA
                     DRIRDWVGPAHIYGFHATIGDALEYPGELVPEIEGRLADIARDTAPFTLTNGRIHDSF
                     RDFPRALVSTFDSPDRAVNRLEERVVTEINALHIGSPEFGTRVAAYT"
     gene            complement(453..1094)
                     /locus_tag="VFI12_02955"
     CDS             complement(453..1094)
                     /locus_tag="VFI12_02955"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7055392.1"
                     /translation="MSIEVFDYRADLRNVYVSPKLRGRFLRHEPGEVGPFHSHDIGDE
                     LFLILEGQCEFEIDGERAVLGPGQLCVARAGQKHEVRVIGDEPMIMFLVVAPHIEPTH
                     TLWDADGKQRPAQYNLTTADQYDRANDAGSVTDLAQTVSTALSQLASDVALAAKTFGN
                     QIGALAAGGTIAKAALDESWLTFLTMHEQLFAFQEAWNLLAGRVFEEYRAIES"
     gene            complement(1105..2196)
                     /locus_tag="VFI12_02960"
     CDS             complement(1105..2196)
                     /locus_tag="VFI12_02960"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET7055393.1"
                     /translation="MERLKIGIIGCGAIGQIQYLPLLRDLREQYEIGGISDLSAELLA
                     NLGRDYGIPADRQFTDYRDLITSSVDCVVVCNTTSYHTDPAIVAMNASKHVLIEKPMC
                     TTIDEAEAIAAAADASGVVAMVGYMKQHDPGYRYAKQFAGQMNDIRFVQVTQLHPDNA
                     LHLQRFHLYKPTDLGPEVRAKMMRDEAELTATALGMTPAQLPSDAAKAFFWVLNSLIH
                     DIGNLNGMFGPPDRVVSADIWENGNCVTTTLEYGAGFRAVASWIDLPDLQTYVETIGV
                     FGSRERVVVSFPTGFSVGLPTEVSTFKMNRNETPYQTQMSWQENPFRLELLRFHECIT
                     QGAEPLTPVRAAVDDVRLAGQIVRTAINA"
     gene            complement(2200..>2535)
                     /locus_tag="VFI12_02965"
     CDS             complement(2200..>2535)
                     /locus_tag="VFI12_02965"
                     /inference="COORDINATES: protein motif:HMM:NF016874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HET7055394.1"
                     /translation="ERTRALTDINQRRDAFHRINLGIGAKNERMIRSVDMLVGVLDGV
                     DVDSGTAGEMGFAYGLGKPVYGLRTDFRLTGDNEAAGINLQLRYFIEQSGGAYVTTVD
                     DLLTALRKA"
BASE COUNT          421 a          780 c          853 g          481 t
ORIGIN      
        1 gtataggcgg cgactcgcgt tccgaactcc gggctgccga tgtgcagcgc gttgatttct
       61 gtcacgaccc gttcctcgag ccggttcacc gcgcgatccg ggctgtcgaa ggtgctgacg
      121 agcgcccggg ggaagtcccg gaagctgtcg tggatacggc cgttcgtgag cgtgaagggc
      181 gcggtgtcgc gggcgatatc ggcaaggcga ccttcgatct ccggcacgag ctcccctgga
      241 tactcgagcg cgtcgccgat cgtggcgtgg aatccgtaaa tatgggccgg cccgacccaa
      301 tcgcgaatcc gatccgcgtg gggctggagc ggctcaggta agggcagttc gcgctcagcg
      361 aagacgtcgt agccgaggat ggcgctggcg atctggtaaa gcctggtctc gggatcgggg
      421 atcaggtagg tcgcgagcct gggcatcggc ggctacgact cgatcgcccg gtattcctcg
      481 aagacccggc cggcgagcag attccacgcc tcctggaagg cgaagagctg ctcgtgcatg
      541 gtcaggaagg tcagccagct ttcgtcaagg gccgctttcg cgatcgtgcc gccggccgcg
      601 agggcaccga tctggttgcc gaaggtcttc gcggccagtg cgacgtcgga ggcgagttga
      661 ctgagcgcgg tcgagacggt ctgggccagg tcggttaccg atccggcgtc gttggcgcgg
      721 tcgtattggt cggcggttgt caggttgtat tgtgcgggcc gctgcttgcc gtctgcatcc
      781 cagagggtgt gcgtcggctc gatgtgcggc gcgacgacca ggaacatgat catcggttcg
      841 tccccgatga cccggacctc gtgtttctgc ccagcccgcg cgacgcagag ctgcccggga
      901 ccgaggactg cccgctcgcc gtcgatttca aactcgcact gcccctccag gatcaggaag
      961 agctcatcgc cgatatcgtg gctgtggaac ggcccgacct cgcccggctc atggcgcaga
     1021 aagcggccac gcagcttcgg cgaaacgtag acgttgcgaa gatcggcccg gtaatcgaag
     1081 acctcgatcg acatggtctg cctcctacgc gttgatggcg gtcctgacga tttggccggc
     1141 cagcctgacg tcatcgaccg cggcccggac cggcgtcagc ggctcggccc cctgcgtgat
     1201 gcactcgtgg aaccggagca gttcgagccg gaacggattc tcctgccagg acatctgcgt
     1261 ctggtacggc gtctcgttcc ggttcatctt gaaggtcgac acctcggtcg gcaggccgac
     1321 agagaagccg gtcgggaaag agacgactac ccgctcccgc gacccgaaga cgccgatcgt
     1381 ctcaacatac gtctgcaggt cgggcagatc gatccagctc gctaccgcgc gaaagccggc
     1441 gccgtattcc agcgtcgtgg tgacgcaatt gccgttctcc cagatgtcgg cggagacgac
     1501 gcggtctggt gggccaaaca tgccgttcag gttgccgatg tcgtggatca ggctgttcag
     1561 aacccagaag aaggccttgg cggcatcgga cgggagctgc gccggcgtca ttcccagcgc
     1621 tgtcgcggtt agctctgctt catcccgcat catcttcgcc cggacctcgg ggccgagatc
     1681 agtcggcttg tagaggtgga accgctggag gtggagggcg ttgtccgggt gcagctgcgt
     1741 gacctggacg aagcggatgt cgttcatctg gccggcaaat tgcttcgcgt aacggtatcc
     1801 gggatcatgc tgcttcatgt acccgaccat ggcgacgaca ccagacgcat cggcggcggc
     1861 ggcgatcgcc tcggcctcgt cgatggtcgt gcacatgggc ttctcgatca gcacgtgctt
     1921 gctggcgttc atcgcgacaa tcgctggatc ggtgtgatac gaggtggtgt tgcacacaac
     1981 aacacaatcg acgctcgatg taatcaggtc gcgatagtct gtgaactgcc gatctgccgg
     2041 gatcccgtag tcccgcccga ggttggccag gagctctgcg gacaggtcgc tgatcccgcc
     2101 gatctcgtac tgctcgcgca ggtcacgcag cagcggcaaa tactggatct gcccaatcgc
     2161 tccgcacccg atgatgccaa tcttcagccg ctccatgggt tacgcctttc gcagcgcggt
     2221 gagcaggtcg tcgacagtgg tgacgtaggc gccgccgctt tgctcgatga agtagcggag
     2281 ttggaggttg atgccggctg cttcgttgtc gccggtgagg cgaaagtcgg ttcgcaggcc
     2341 gtagaccggc ttgccgagcc cataggcgaa gcccatctcc ccggccgtcc cggaatcgac
     2401 gtccacgccg tccagcaccc cgaccagcat gtcgaccgag cggatcatgc gctcgttctt
     2461 ggcgccgatc ccgaggttga tccgatggaa ggcgtcgcgc cgctgattga tgtcagtcag
     2521 ggcgcgggtg cgctc
//
LOCUS       DASSGX010000166         3078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_466415, whole genome shotgun sequence.
ACCESSION   DASSGX010000166 DASSGX010000000
VERSION     DASSGX010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466415"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VFI12_02970"
     CDS             complement(<1..315)
                     /locus_tag="VFI12_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055395.1"
                     /translation="MSATGSSVARGPAARRFDLATALTHLILIVACLAILLPVLWTAR
                     TSFANRRIAYDPGTLVFAPTLDNYGKIFAEEPFLKYFWNSLWIGLASALFSLAVGSLA
                     AYS"
     gene            complement(448..2400)
                     /locus_tag="VFI12_02975"
     CDS             complement(448..2400)
                     /locus_tag="VFI12_02975"
                     /inference="COORDINATES: protein motif:HMM:NF008559.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HET7055396.1"
                     /translation="MAQAVTRTRYPLEPLSVDELAEAVAILRTGAPDGPWDDRRYRFV
                     EVALREPAKAAILAAEAAGTGEELPREARAVFIDRAERVSVVAIISLTEGRITAWEAI
                     PVGQAPFTLEEILECERVVRQDPDFQDAMHRRGITDMDLVWVDPWPFGVYPDETDLEG
                     RRLTRGLVWVRANPDDANGYAHPVENVIVIFDLHEMAVVRVEDHGVAPVPGQTANYGP
                     DDILSIRADLKPIEITQPEGPSFTVEGMLVRWQKWRLRVGFTPREGLVLHTVGWEEDG
                     LVRPILHRAALSDMVVPYGDPAPTHARKNTFDGGELNFGALVNSLTLGCDCLGEIHYF
                     DVALVDQDGKPYTLKNAICMHEEDYGVLWRHLNMRTGRTDVRRSRRLVISSFSTIGNY
                     DYGIFWYLYQDGTIQCEVKLTGILSTGAVAPGETPRYGQLLNADGLYGPIHQHFFNFR
                     LDLDVDGPVNTVYEEHAEADPAGPENPLQAAFRTVRTALRRESEAQQLIDPLRGRVWR
                     VVNPERLNALGEPVGYRLVPHGNVQALAGADASVSKRAAFITKHLWVTPHDDRERHAA
                     GDFPAQHPGGAGLPEWTAADRPIAETDVVLWYTLGSHHAPRPEDWPVMPVSYAGFLLQ
                     PVGFFPANPALDVPPPTPHGNGHCCD"
     gene            complement(2534..>3078)
                     /locus_tag="VFI12_02980"
     CDS             complement(2534..>3078)
                     /locus_tag="VFI12_02980"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET7055397.1"
                     /translation="YARCVNTGAQPGAAGADLNDTMATYSQGKAFSMISYMFMLSVFN
                     DDEASTVKGVNTMTVMPGGHGLTGAWSWGLPISSPNPDAGWEFIKWVESPDIAMQRAL
                     AGGVPAQQAPYENQEFLDKYPWMPQALQMIGSGQGLPAVTKQAQLVEIMGRHLSDVVS
                     GAQTAQAGMDAAAEELKDLL"
BASE COUNT          495 a         1042 c         1022 g          519 t
ORIGIN      
        1 cgagtaggcc gccagcgagc cgaccgccag gctgaacagc gccgaggcga gcccaatcca
       61 aagcgagttc cagaagtatt tcagaaacgg ctcttcggcg aagatcttgc cgtagttgtc
      121 cagggtcggg gcgaagacga gtgtgcccgg atcgtaggcg atgcgccggt tcgcgaacga
      181 cgtccgagcc gtccagagga ccggcagcag gatggcgaga caggcgacga tcaggatcag
      241 gtgcgtgagg gccgtggcga gatcgaagcg gcgcgcagcc ggcccgcgcg ccacggacga
      301 acctgtcgcg ctcacgctcg ctcttcccca accttgggcg ccgtcatcgc tgctcgcttt
      361 cccgtctctc cagttcggaa gcctaggcag gcgcgaatct actgtcaatg cgtcggcggt
      421 cgagacagtg catcgccccg cgccgcgtta gtcgcagcag tgcccattgc cgtgcggcgt
      481 cgggggcggg acgtcgagcg ccgggttggc cgggaagaac ccgaccggct gcagcagaaa
      541 tccggcgtag gagaccggca tcaccggcca gtcctcgggc cgcggcgcgt ggtggctgcc
      601 gagcgtgtac cagagcacca catccgtttc cgcgatgggg cggtcggcag cagtccactc
      661 cggcagcccc gccccgcccg ggtgctgagc cgggaaatcg cccgccgcgt gccgctcgcg
      721 gtcgtcgtgc ggcgtcaccc agagatgctt ggtaatgaac gccgcccgct tgctgacgct
      781 ggcgtcggcg ccggccagcg cctggacgtt gccgtgcggc accagccggt acccgacggg
      841 ttcgccaagc gcgttgagtc gctccgggtt gacgacccgc catacgcgac cacgcaaggg
      901 atcaatcaac tgctgtgcct ccgactcgcg ccgcagcgcg gtgcggacag tgcggaacgc
      961 cgcttgaagc gggttctccg gccccgccgg gtcggcttcg gcgtgctcct cgtagacggt
     1021 gttcactggc ccatcgacat ccaggtcgag ccggaaattg aagaagtgct ggtggatcgg
     1081 gccgtagagt ccatccgcgt tgaggagctg gccgtagcgt ggcgtctccc ccggcgccac
     1141 cgcgccagtc gagaggatgc cggtcagctt gacctcgcac tggatcgtgc cgtcctggta
     1201 gaggtaccag aagatgccgt agtcgtaatt gccgatggtc gagaaggacg agatgacgag
     1261 ccggcgcgag cggcggacgt cggtgcgccc ggtgcgcatg ttgagatgcc gccagagaac
     1321 gccgtagtcc tcttcgtgca tacagatcgc gttcttcaag gtgtacggct tgccgtcctg
     1381 atcgaccagc gccacatcga agtagtggat ctcgcccagg cagtcgcagc cgagcgtcag
     1441 cgaattgacg agggcgccga agttcagctc gccaccatcg aacgtgttct tgcgggcgtg
     1501 ggtcggcgcc gggtcgccat acggcacgac catgtcggac agggcagcgc ggtggaggat
     1561 ggggcggacc agaccatcct cctcccagcc aactgtgtgc aggaccagcc cttcccgcgg
     1621 cgtaaagccg acgcgcaggc gccatttctg ccagcgcacg agcatcccct cgacggtgaa
     1681 gctcggcccc tccggttgcg tgatctcgat cggcttcaga tcggctcgta tcgagaggat
     1741 atcgtccgga ccgtagttgg cggtctgccc tggcactggc gccacgccgt ggtcctcgac
     1801 gcggacgact gccatctcat gcagatcgaa gatcacgatc acgttctcga ccgggtgggc
     1861 gtagccgttg gcgtcgtcgg ggttggcccg cacccagacg agtccgcgtg tcagccgccg
     1921 gccttcgaga tcggtctcat ccgggtagac cccgaacggc cagggatcga cccagaccag
     1981 atccatatcg gtgatgccgc ggcgatgcat cgcatcctga aaatcggggt cctgccggac
     2041 cacccgctcg cactcgagga tctcttcgag cgtgaatggc gcctgtccga ccgggatcgc
     2101 ctcccacgcg gtgatgcgcc cctccgtgag cgaaatgatc gctacgaccg acacccgctc
     2161 cgcccggtcg ataaacaccg cccgcgcctc gcgcggcagc tcttcgccgg tccccgccgc
     2221 ctcggccgcc aggatcgcgg ctttcgccgg ctcccgcagc gcgacctcga cgaaccgata
     2281 gcgacggtca tcccaaggtc catccggagc cccggtgcgg aggatggcga ccgcttccgc
     2341 caactcgtca accgagagcg gctccagtgg ataccgcgtg cgcgtcactg cctgtgccat
     2401 gtgtgccttt ccgtgggtga tagggggttg gggttttggg gtgttagggg gtgatcggct
     2461 gccggccggc gagccttgcc gtctcgccgc tgtctcccga tcaccccatc accccgacac
     2521 cctgttaccg tcgctacagc aggtccttca gctcttcggc cgcggcgtcc atccccgcct
     2581 gggcggtctg agcgccggag accacgtcgc tcaggtggcg gcccatgatt tcgaccagtt
     2641 gcgcctgttt ggtcacggcc gggaggccct gcccggagcc gatcatctgc aacgcctgcg
     2701 gcatccaggg gtacttgtcg aggaactcct gattttcgta cggcgcctgc tgcgccggca
     2761 cgccgcctgc gagcgcccgc tgcatggcga tatcgggcga ctccacccac ttgatgaact
     2821 cccaaccggc gtccggattc ggcgacgaaa tcggaagccc ccagctccag gccccggtca
     2881 aaccgtggcc gcccggcatg acggtcatcg tgttgacgcc cttgaccgtc gacgcctcat
     2941 cgtcgttgaa gaccgagagc atgaacatgt aggagatcat gctgaaggcc ttcccttgac
     3001 tgtacgtggc catcgtgtcg ttcaggtcgg cgccggcggc gcccggctgc gcgccggtgt
     3061 tgacgcagcg cgcataga
//
LOCUS       DASSGX010000167         6419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_903194, whole genome shotgun sequence.
ACCESSION   DASSGX010000167 DASSGX010000000
VERSION     DASSGX010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6419
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903194"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VFI12_02985"
     CDS             complement(<1..374)
                     /locus_tag="VFI12_02985"
                     /inference="COORDINATES: protein motif:HMM:NF037033.4"
                     /note="Related to clavulanate biosynthesis protein Cpe,
                     which has an isomerase-like N-terminal domain and a
                     beta-lactamase-like C-terminal domain; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cpe/LpqF family protein"
                     /protein_id="HET7055398.1"
                     /translation="MVDCMCSHDARVSRRSFLLAAAGLVVPAVVRADPVDPITPVEPV
                     PSAFSIPDTAAGRQLAWVLEQVNRSGRGLSLREIREHVDTGFLLALPAEQIRAVFRTY
                     VGPSGPLVPARFEGGVSETRAYT"
     gene            complement(522..1856)
                     /locus_tag="VFI12_02990"
     CDS             complement(522..1856)
                     /locus_tag="VFI12_02990"
                     /inference="COORDINATES: protein motif:HMM:NF037527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055399.1"
                     /translation="MATNPIRRIRKREHETPFDLDIRSSLRRLAELPPSPGIPYLTVS
                     LDWRPEGSDPARVALGDVKPSQRRMLPRFPTTSRRPSRLEVEQTFKELIAEFGPRGEA
                     FDSLTADVARLGEVLDDVDPSAQGIYVVACSAKGVFEELSLGLPIATSATVGPTPALS
                     TLARLDDDYPPYAVLLADQHEATLSFITQAAVSESVSLDSSGYPRKQKQGGPNQRRYQ
                     NRADERVAAFARGIADETRQALDETGVGMLIIAGDEVITSALDAVLHETVKERIITVI
                     RLDNTATEQQVIDATQPLVRQEERDREREAVEELQNRIGADAEAVTGAERTLATLQGG
                     RVDSLIMVDDLALDGWADFAMGLYGTGDPPAEHPAGGDVADIVPVDLDEELVRLAFLG
                     AADVQIVKTASPDRAAETDTIPADSATASRSDPAMALDKLGGVGALLRYSIF"
     gene            complement(2044..2820)
                     /locus_tag="VFI12_02995"
     CDS             complement(2044..2820)
                     /locus_tag="VFI12_02995"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7055400.1"
                     /translation="MDLRLDGKVALVTGSSKGIGKGIAEEFARSGADVTITYEGDEAG
                     AEEVAGYVRSLGRRALIVQVDVADEAAVDRLYEAHLAEFGRLDILVNNAAVMSHGRIA
                     EISTAQWRKVLAANLDGAFFCMSRAARHMIAQGGTGRIITISSVHEDATTDGGGPYSV
                     SKAGLRNLMRTMAVELGEYGITVNGIAPGMTVTPGMNSEIYNDEREREKRAQLIVLKR
                     AGYPQDIANMAVFLASDAASYCTGATYLVDGGWMLTRPPV"
     gene            complement(3040..4125)
                     /locus_tag="VFI12_03000"
     CDS             complement(3040..4125)
                     /locus_tag="VFI12_03000"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HET7055401.1"
                     /translation="MRALLDRRGLDACVLFDRSEIYYFTGFLISDWVSQPAALYVEIG
                     GYSLLVCGTDEGEALVDERVLYEPHVLYTRNLDMVRQIAARMRERLTGRSVSRLGWRA
                     EVLPRVIGLALDAALAPDEWIEIDADIEDMERSKDPDEVECIRTSIACTLAAYDAAQL
                     AIAPRVSELIVLQAAQRSAILRAGEQVFHSGDYRAGAFGGFARAHPISENDLYIIDAW
                     TEYRSYWADLCRTFAIGTPTPLQREVYAYIASILVDVEGVLKPGVHGTEVWHWVDRRL
                     REHPLFRETGLVHHAGHGVGLRSHEGPDLNRDRGDVLRPGDVVSVEPGAYAPKLRPGF
                     RLENMFLITETGCELLSEYPLSLDRQV"
     gene            complement(4248..5042)
                     /locus_tag="VFI12_03005"
     CDS             complement(4248..5042)
                     /locus_tag="VFI12_03005"
                     /inference="COORDINATES: protein motif:HMM:NF025691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HET7055402.1"
                     /translation="MDRGPSEGAVYCTNCGHQLAPDDRFCPECGTSRPTFSPRETNPP
                     AFTSSITTATTLGEFTGAPPMMFGTMAPAPVGISSPLLEYDVEYPEHLSRLLIFVKWL
                     LIIPHLFVLMFVGLVQYVVTVIAFFAILLAGRYPRGMWDFSVSVLRWASNVNAYTNLQ
                     RDEYPPFSGTAPYPVHLALAYPERLSRLLIFVKWLLVIPALIVYTIIAWIAGIVVFLA
                     FFAILFTGRYPRGLFDFITGTMRWGNRISAYFLLLTDAYPPFRMGP"
     gene            5187..5765
                     /locus_tag="VFI12_03010"
     CDS             5187..5765
                     /locus_tag="VFI12_03010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET7055403.1"
                     /translation="MPEKSKPVLQIIIASTRPGRVGLPVGTWFEQCAIAHGAFDVQVA
                     DLAEIDLPFLDEPNHPSQQRYTKQHTKDWSARIAASDAFAFVMPEYNHSFTAPLKNAI
                     DYLVKEWAFKPVGLVSYGGISGGLRAVQALKPVLSGLRMVPLTEAVVIQFVASLIEDD
                     GHFEPTTSMANSVKPMLDELAREVPVLKQLRK"
     gene            complement(5837..6178)
                     /locus_tag="VFI12_03015"
     CDS             complement(5837..6178)
                     /locus_tag="VFI12_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055404.1"
                     /translation="MAVYSGNSGSILFEGVIVGAVAEWRLLLVQHNGDARWTGVLTWQ
                     WLSLPPGGRDEDEFEIELQDGSETPSYNLSGRTTFTGIDPDKRTLEFRGQGDLAGAPD
                     PGPDQSEIAIT"
BASE COUNT         1134 a         2149 c         2080 g         1056 t
ORIGIN      
        1 gtataggcgc gcgtctcgct gaccccgccc tcgaaccggg ccgggaccag cggaccgctc
       61 ggccccacat atgttcggaa gacggcccgg atctgctccg ctggcagcgc gagcaggaac
      121 ccggtatcga catgctcccg gatctcgcgc aggctcagtc cccgaccgct gcgattgacc
      181 tgctcgagca cccacgccaa ctgccgcccg gctgccgtat cggggatcga gaaagcggat
      241 ggtaccggct caaccggtgt aatcggatcg accggatcgg cccggacgac ggctggcacg
      301 accagtccgg ccgcggccag cagaaaggag cgccgggaaa cccgggcgtc gtgggagcac
      361 atgcaatcga ccatctgtca ctcagcgggg cagcctcacc gtatctcact accccaggaa
      421 cgacaatccc ccatccgtcg tttcccgcga ccgatgctca accattcgtg ccgagccgaa
      481 actccctggc agccgaagtt gcgccggccg ccggggttcg tctagaagat ggaataccgc
      541 aggagcgcgc cgactccgcc cagcttgtca agcgccattg ccgggtcgga tcgactggct
      601 gttgcactgt cggccgggat cgtgtcagtc tccgcggctc gatccgggct tgccgtcttg
      661 acgatctgca cgtcggcggc gcccaggaag gcgaggcgga cgagctcctc gtcgagatcg
      721 accgggacga tgtccgcgac gtcgcctccg gccggatgct cggcgggcgg gtcgcccgtg
      781 ccgtagagcc ccatcgcaaa atcggcccag ccgtcgaggg caaggtcgtc gaccatgatc
      841 agggaatcga cccggccgcc ctggagcgtc gccagggttc gctcggctcc ggtaacggcc
      901 tcagcgtcgg cgccgatccg gttctggagc tcctccacgg cctcccgctc ccggtcccgc
      961 tcctcctgcc ggaccagcgg ctgggtggcg tcgatcacct gctgttcggt ggccgtgttg
     1021 tcgagccgga tcacggtgat gatccgctcc ttgaccgtct cgtgcagcac cgcatcgagc
     1081 gcgctcgtga tcacttcgtc accggcaatg atcagcatgc caacgccggt ctcgtcgagt
     1141 gcctgccgcg tctcgtcggc gatgccccgc gcgaaggccg cgacccgttc atcggctcgg
     1201 ttctgatacc ggcgctggtt cgggccgccc tgcttttgct tgcggggata gccgctgctg
     1261 tccagcgaca cgctctcgga tacggctgcc tgggtaatga acgagagggt cgcttcgtgc
     1321 tgatcggcaa gcaacacggc atagggcgga tagtcgtcat caaggcgagc cagcgtcgac
     1381 aacgcaggcg tcggtcccac ggtggcgctg gtcgcgatcg gcaagccgag cgagagctcc
     1441 tcgaagacgc ccttcgccga gcacgcgacg acgtaaatgc cctgggcaga cgggtcgacg
     1501 tcatccagca cctcgcccaa gcgagcgaca tcggcggtca ggctgtcgaa cgcctcgccg
     1561 cgggggccga attccgcaat cagctcctta aacgtctgct cgacctccag gcgggacggc
     1621 cggcggctcg tggtcgggaa gcggggcagc atccgccgct gggacggctt gacatcgccg
     1681 agcgccacgc gcgccggatc cgagccttcc ggccgccagt ccaggctcac cgtcaggtac
     1741 ggaatcccgg gcgagggagg cagctccgcc aggcgtcgca gcgacgagcg gatatcgaga
     1801 tcgaaggggg tctcatgctc tcgcttgcgg atccggcgga ttgggttggt ggccatgagc
     1861 gtcctcctgg tgacattaat gggacggcgg gaatggcgcg gcgtgcctac gcccgactgc
     1921 agccgtcgtt cggcccattg cgtctccagt gtcggtcagg tggtcccgcc gaacgcggac
     1981 ttgagccaca atcgggcaaa gcatagccca ttggcggtga gcaaaacggc cgttgatcag
     2041 ggatcagacc ggggggcggg tgagcatcca gccgccgtcg accaaatacg tcgcgcctgt
     2101 gcagtagctg gcggcgtcgc tggccaggaa gacggccata ttcgcgatgt cctgcgggta
     2161 accggcgcgc ttcagcacga tcaactgcgc ccgcttctcc cgctcccgct cgtcgttgta
     2221 gatctcggaa ttcatgccgg gcgtgaccgt catccccggc gcgatcccgt tgacggtgat
     2281 cccatactcg ccgagctcaa ccgccatcgt ccgcatcagg ttgcgcagcc ccgctttgga
     2341 gacggagtag ggaccgcccc cgtcggtggt ggcatcttcg tggaccgatg agatggtgat
     2401 gatcctgccc gtgccgccct gggcaatcat gtgccgcgcc gcccggctca tgcagaagaa
     2461 ggcgccgtcg agattcgcgg ccaggacctt tcgccattgg gcggtcgaga tctccgcgat
     2521 ccggccgtgg gacatgactg cggcgttgtt gaccaggatg tcgagccggc cgaattcggc
     2581 caggtgcgcc tcgtacaacc gatcgaccgc tgcctcgtcc gcgacatcga cctggacgat
     2641 cagcgcccgc cggcccaggc tccggacata tccggcgacc tcttccgccc cggcctcgtc
     2701 gccctcgtag gtgattgtca cgtccgcccc ggagcgggcg aactcctcgg cgatgccctt
     2761 gccgatgccc ttgctggaac cggtgacgag cgccaccttg ccatcaagcc ggagatccat
     2821 ctgcgatcgt gctccttacg tcccgatgca cggtgtggtg tagcgcgggg atactttgcc
     2881 ccctggtagg gagagtcgga gagggggtca cgccccgccc gaaccccgtg acatccacgc
     2941 cgttgaaggg aaccagtccc aatacccggt cgatacgttc taccgcgtgc ggggaggcac
     3001 aatgcggggc acggtgaccc cgcgctacgt tctttcgttc tagacctggc gatcgagcga
     3061 gagcgggtac tcggagagca gctcgcagcc cgtctcggtg atcaggaaca tgttctcaag
     3121 ccggaagccc ggccgcaatt tgggagcata cgccccgggc tcgaccgaaa cgacatcccc
     3181 cggccgcagc acatcgcctc gatcccggtt caggtccgga ccttcgtgcg agcgcaggcc
     3241 gacgccgtgg cccgcgtggt gaacgagccc ggtctcccgg aagaggggat gctcgcggag
     3301 ccgccgatcg acccagtgcc agacctcggt gccgtggaca cccggcttca gcacaccctc
     3361 gacatcgacc aggatgctcg cgatgtacgc ataaacctcg cgctggaggg gagtcggcgt
     3421 gccgatcgcg aaggtgcggc agaggtctgc ccagtagctg cggtactcgg tccaggcgtc
     3481 gatgatgtag agatcgttct cgctgatcgg gtgcgcccgg gcaaacccgc cgaacgcgcc
     3541 ggcccggtaa tcgcccgaat gaaagacttg ctcgcccgcc cggaggatgg cggatcgttg
     3601 agcggcctgc agcacgatca gctccgacac gcgcggcgcg atcgcgagct gggccgcgtc
     3661 gtacgccgcc agcgtgcagg caatcgacgt gcggatgcat tcgacctcgt ccgggtcctt
     3721 gctccgctcc atatcctcga tgtccgcgtc gatctctatc cactcgtccg gggcgagcgc
     3781 agcgtcgagc gcgaggccga tgacccgggg caggacctcg gcgcgccagc ccaggcggga
     3841 aaccgatcgc ccggtcaatc gctcccgcat gcgggctgca atttgccgga ccatgtcgag
     3901 gttgcgagtg tagagcacat gcggctcgta gaggacccgc tcgtcgacca gcgcctcgcc
     3961 ctcgtcggtg ccgcatacca acagggaata gccgccgatt tcgacataga gcgcggccgg
     4021 ctggctcacc cagtccgaaa tcaggaagcc ggtgaagtaa tagatctcgc tccggtcaaa
     4081 gaggacacac gcgtccagcc cgcgacgatc gagcagcgcc cgcaaacgat cctgccggga
     4141 ttggcacccg gccttcgtca acacagcatc aacccccaca gacgacggcc gtggccgtca
     4201 tcctagcaag ccgaatcgcg cccggcaatc gggacaaaca cctatgacta tggccccatc
     4261 cggaagggag gatacgcgtc ggtcagcagc aggaaatagg cgctgatgcg gttcccccac
     4321 cgcatcgtgc cggtgatgaa gtcgaacagc ccgcgcgggt agcgaccggt gaagaggatc
     4381 gcgaagaagg ccaggaacac cacgatcccg gcgatccagg cgatgatggt gtagacgatc
     4441 agcgccggga tcaccaagag ccacttgacg aagatcagga gccggctcaa tcgctccggg
     4501 taggccagcg cgaggtggac ggggtacggc gccgtgccgc tgaagggtgg gtattcgtcc
     4561 cgctgcaaat tcgtgtacgc gttgacgttc gatgcccagc gcaggacgct cacagagaag
     4621 tcccacatcc ctcgcgggta gcgcccggcg agcaggatcg cgaagaaggc gatgactgtc
     4681 accacgtact ggaccagccc gacgaacatc agcacgaaca agtgcgggat gatcagcagc
     4741 cacttcacga agatcagcaa tcggctgagg tgctcgggat actcgacgtc atattcgagc
     4801 agcggcgagc tgatcccaac cggggccggc gccatcgtcc cgaacatcat cggcggggcg
     4861 ccggtgaact cgccgagcgt ggtcgcggtc gtgatgctgc tcgtgaaggc gggcggattc
     4921 gtctcgcggg gcgagaacgt gggccgggac gtgccgcatt ccgggcaaaa ccggtcgtcc
     4981 ggggcaagct ggtgcccgca gttcgtgcag taaacggcgc cttcgctcgg gccgcgatcc
     5041 atacccatgc tccttgtcgg tatcaggaca atcgcctgat tctacgcgac tgctcgcacc
     5101 gatagcctgc gcacaccggc acgccggccc ggctccctat ccttgctctc cggaaattga
     5161 ccggtcgctc acggaggaga ggcagcgtgc cagagaaatc gaagccggta ttgcaaatca
     5221 tcatcgccag cacgcgcccg ggcagggtcg gtctgccggt cggcacgtgg ttcgagcagt
     5281 gcgcgatcgc gcacggcgca ttcgacgtgc aggtcgccga cctggcggag atcgatctgc
     5341 ccttcctcga cgagccgaat caccccagcc agcagaggta cacgaagcag cacacgaagg
     5401 actggagcgc gcggatcgcc gcgtccgacg cctttgcctt cgtcatgccg gagtacaacc
     5461 actccttcac ggcgccgctc aagaacgcga tcgactacct ggtcaaggaa tgggccttca
     5521 agccggtcgg gctggtcagc tacggcggca tctccggtgg attgcgggcg gtgcaggcgc
     5581 tcaagccggt cctctccggc ctgcgaatgg tgccgctgac cgaggcggtc gtcatccagt
     5641 tcgtggcgtc gctgatcgag gacgacggcc acttcgagcc gacgacctcc atggccaact
     5701 ccgtcaagcc gatgctcgac gagctcgccc gggaagtacc ggtgttgaag caactgagga
     5761 agtagccggt tgtcagtagc cagcaactct tttgccttca ctggctaccg gctactggca
     5821 tctggctact gcgtcgtcac gtgatcgcaa tctctgattg atcggggccg gggtcggggg
     5881 cgccggcgag gtcgccctgg ccacggaact cgagcgtgcg cttgtcgggg tcgatgcccg
     5941 taaaggtcgt ccggccggag aggttgtagc tcggcgtttc ggagccgtcc tgcagctcga
     6001 tctcgaactc atcctcgtcc cgtccgccgg gcggcaggct gagccattgc caggtcagca
     6061 ccccggtcca gcgcgcatcg ccgttgtgct gcacgagtag cagccgccat tccgccaccg
     6121 cgccgacgat cacgccctcg aacaggatcg aaccggaatt cccgctgtac acagccatac
     6181 agggccgctc gcttgccatc gcctcgtcgc gaatcaggta cttttgcatc cgtaccggag
     6241 agcataggga gcaagcaggg atttatcaat gacagacgac ccgacaacgc tgatggcctt
     6301 cgtggagatt ccgcgcggca gccgcaacaa gtacgagtac gacgaggcaa ccaggagctt
     6361 ccggctcgac cgcgtgctct attcgtcggt ccattatccg accgactacg gcttcatcc
//
LOCUS       DASSGX010000168         2018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_249832, whole genome shotgun sequence.
ACCESSION   DASSGX010000168 DASSGX010000000
VERSION     DASSGX010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249832"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2018
                     /gene="topA"
                     /locus_tag="VFI12_03020"
     CDS             <1..>2018
                     /gene="topA"
                     /locus_tag="VFI12_03020"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HET7055405.1"
                     /translation="SGYSVTASMGHIRDLPKSTMGVDLEHDFTPRYMVPRDKSKLVKE
                     LKESVESANELILATDPDREGEAIAWHLVEATNPQGKPVKRVVFHEITPDAVRAAMEN
                     PREIDMDLVDAQQARRVLDRIVGYTVSPLLWKKVKRGLSAGRVQSAALRIVVDRERVI
                     DAFTPVEYWSLDAYLHGETNGKQTPSFKAGLHRIGKDKAELKTEEQTNSVVRDLDSAT
                     YRVTSVTKRESQRRPSAPFTTSTLQQEASRKLGYNVRRTMQIAQELYEGIDLGADGGT
                     QGLITYMRTDSTNVSAVAQQAARAVISVKFGPEYVPATPPVYARKAKGAQEAHEAVRP
                     TAPQRDPASVKKYLSPQQFRVYQLIWQRFLASQMAPAQLDGTTIDIAAGRNLPANAKD
                     APYTFRATGSVVRFPGFMAVYTQGRDEGDADELDDGALPALTAEQLLALEKLVPEQHF
                     TQPPPRFTEAMLVKALEEQGIGRPSTYAPTIATLVARSYVAIEEKKLIPTELGLVVSD
                     LLAEHFPNIFDVGFTSQLENELDEIAAGERAWVPTLREFYGPFDETMKKAETTIERVK
                     LKDEPAGVDCEKCGRPMVIKMGKYGKFLACSGFPECRNARPLLATIGMTCPKCHEGDV
                     VERRSKRGRIFYGCSRYPECDFSEWNKPVDHQCPKCGNPYMTEVGKRG"
BASE COUNT          396 a          663 c          671 g          288 t
ORIGIN      
        1 agcggctact cggtgacggc ctccatgggt cacatccgcg acttgcccaa gagcacaatg
       61 ggcgtcgacc tggagcacga cttcacgccc aggtacatgg tgccgcgcga caagtccaag
      121 ctggtcaagg aactcaagga atcggtcgaa agcgcgaacg agctgatcct cgcgaccgac
      181 cccgaccgcg agggcgaggc catcgcctgg cacctcgtcg aagcgaccaa cccgcagggc
      241 aagccggtca agcgcgtcgt gttccatgag atcacgccgg acgcggtccg ggcggcgatg
      301 gagaaccccc gcgagatcga catggacctg gtcgatgccc agcaggcgcg ccgcgtcctg
      361 gaccggatcg tcggttacac cgtcagcccg ctgctctgga agaaggtcaa gcgcggcctc
      421 tccgccggca gggttcaatc ggccgcgctc cggatcgtcg tcgaccgcga gcgcgtgatc
      481 gacgccttca cgccggtcga atactggtcg ctcgacgcct acctgcacgg ggagacgaac
      541 ggcaagcaga cgccgtcctt caaggccggt ctgcaccgga tcggcaagga caaggccgag
      601 ctgaagaccg aagagcagac caactcggtc gtgcgtgacc tggacagcgc gacctaccgc
      661 gtcacctctg tgaccaagcg cgagtcgcag cggcgaccct cggccccgtt cacgaccagc
      721 acgctccagc aagaggcctc gcgcaagctc ggctataacg ttcgccgcac gatgcagatc
      781 gcgcaggagc tatacgaggg gatcgacctc ggcgccgacg gcggcaccca ggggttgatc
      841 acctacatgc gaaccgactc gaccaacgtt tcggcggtgg cgcagcaggc ggcgcgggcc
      901 gtgatcagcg tcaagttcgg gccggaatat gtcccggcca cgccacccgt ctatgccagg
      961 aaggcgaagg gcgcgcaaga ggcgcacgag gcagtccggc cgaccgcccc gcaacgggat
     1021 ccggccagtg tcaagaaata cctctcgccg cagcaattcc gggtgtacca actgatctgg
     1081 cagcgattcc tggccagcca gatggcgcct gcccagctcg acgggacgac gatcgacatc
     1141 gccgccggcc gcaatcttcc tgccaacgcc aaggacgcgc cgtacacatt ccgggccacc
     1201 ggttcggttg tcaggttccc cggcttcatg gccgtctaca cgcagggccg tgacgagggc
     1261 gacgcggacg agctggatga tggcgcgctg ccggcgctga ccgccgagca gctcctggcg
     1321 ctcgagaagc tcgtgcccga gcagcacttc acccagccgc ccccccggtt caccgaggcc
     1381 atgctggtca aggcgctcga ggagcagggg atcggccggc cgagtacgta cgcgccgacc
     1441 atcgcgacgc tggtggcgcg gtcgtacgtc gcgatcgagg agaagaagct gatcccgacg
     1501 gagttgggcc tggttgtcag cgacctgctg gccgagcact tcccgaatat cttcgatgtc
     1561 ggcttcacgt cgcaactcga aaacgagctg gatgagatcg ccgccggcga gcgagcctgg
     1621 gttccgacgc tcagagagtt ctacggtccg ttcgacgaga cgatgaagaa ggccgagacc
     1681 acgatcgagc gggtcaagct caaggatgag ccggccggtg tcgattgcga gaagtgcggg
     1741 cggccgatgg tcatcaagat gggcaagtac gggaagttcc ttgcgtgcag cgggttcccg
     1801 gagtgccgga atgcgcgccc gctcctggcc acgatcggca tgacctgccc gaagtgccac
     1861 gaaggggacg tggttgagcg gcgatcgaag cgtggccgga tcttctacgg ctgcagccgg
     1921 taccccgagt gcgacttcag cgagtggaac aagccggtcg accaccagtg tccgaagtgc
     1981 gggaacccat acatgaccga agtcggcaag cgcggtca
//
LOCUS       DASSGX010000169         2389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_32148, whole genome shotgun sequence.
ACCESSION   DASSGX010000169 DASSGX010000000
VERSION     DASSGX010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32148"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(232..783)
                     /locus_tag="VFI12_03025"
     CDS             complement(232..783)
                     /locus_tag="VFI12_03025"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HET7055406.1"
                     /translation="MAAQWLVHRPTVAEAPPEGAPSWVEAIPAARRRHLRVWLWLGAA
                     LTTSALVVGGITRLTEAGLSIVDWAPIVGAVPPLNDAAWHEAFARYQQYPEDVKLRLD
                     MTLTEFKYIRFWEYLHRMIGRLIGMVFLVPFIWVWIRGSFHAPLIRRVLLLFALGGPQ
                     GLIGWDMVSSGMVDRPEVSHYRP"
     gene            complement(976..1048)
                     /locus_tag="VFI12_03030"
     tRNA            complement(976..1048)
                     /locus_tag="VFI12_03030"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1013..1015),aa:Arg,seq:tct)
     gene            complement(1179..1415)
                     /locus_tag="VFI12_03035"
     CDS             complement(1179..1415)
                     /locus_tag="VFI12_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055407.1"
                     /translation="MFESESLLRHETIGQRLETCDICGQPAAELSTIVIDGDRAGTEP
                     VEEMRVCPGCRERLYRDELSVDDELTPNIEPNDD"
     gene            complement(1602..>2389)
                     /gene="glpX"
                     /locus_tag="VFI12_03040"
     CDS             complement(1602..>2389)
                     /gene="glpX"
                     /locus_tag="VFI12_03040"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HET7055408.1"
                     /translation="PMLYTGEEVGTGEGPEVDIAVDPVEGTRLVAQGRPSSIVIMAVA
                     ERGSLYGWQDIAYMEKIAVGPEGAGAIDITKSVQENLVNVSKAKGVNIDDLTVVILDR
                     PRHESLVNQVRDAGARMKLITDGDVAAALMAAMHGTGIDLLMGIGGSPEAVIAACALK
                     CVGGDMQCRLWPRNDEERQVAKAQHIDLSRIYTLNDLVTSENIFFAATGITDGELLSG
                     VHYFSDGATTQTLVMRATSGTVRYINSTHKLSKLRSYYHIPFD"
BASE COUNT          416 a          794 c          777 g          402 t
ORIGIN      
        1 gacgcgcgcc tcgcccgacg aggttgtcat gggccacagc catgccgcag cgatccggga
       61 ttcgggaggg aaggtcgcgt gattggggca aagatcggta tcaagcgcac cactcccttc
      121 gcaagccagc acgtccacca agcacggcgc aacgtctggt gtgttggtgc cccggacgcg
      181 atgcgagcgc gcgacttacc gctgagaagg cgaaaagcag agtccttagg gtcatggtcg
      241 gtagtggctc acttccggcc ggtccaccat accgctgctc accatgtccc agcctatcag
      301 cccttgcggg ccgccgagcg cgaagagcaa tagcacccgc ctgataagcg gagcgtggaa
      361 ggacccgcgg atccagaccc agatgaaggg gaccaggaag accatgccga tgaggcgccc
      421 gatcatccga tgcaggtact cccagaagcg gatgtacttg aactccgtga gggtcatgtc
      481 cagccggagt ttcacgtcct ccgggtactg ctggtagcgg gcgaacgcct catgccaggc
      541 cgcgtcgttc agcggcggga ccgcgccgac gatcggcgcc cagtcgacga tcgagagccc
      601 ggcctcggtc aggcgggtga tcccgcccac gacgagcgcg ctcgtcgtca gcgcggcgcc
      661 gagccatagc cacacgcgga gatgccgccg tcgcgccgct ggtatcgctt cgacccacga
      721 gggggcgcct tcgggcggtg cttccgcgac cgtcggccgg tggacgagcc attgggctgc
      781 catcttgacg cactccattc accccacggc ccgtccggcg cggatcgacc ggcccgggcc
      841 caatgcagga gagcgtagcg caccagcgga gcgcggagac gcgactccgg agaaagggga
      901 acgggcagta caggaacaag tgccgatttc cgagacgcga ggtgcgccgg cgagattggc
      961 aatgaatggg accggtaccc caggcgcgat tcgaacgcgc ggcctaccgc ttagaaggcg
     1021 gttgctctgt ccgctgagct actggggcgc gatgtcggac ggccaatccg gcagaatccg
     1081 gcgggtaccc agcgaacagt ataccccgga cgccgcggcc ggttactgat ggtaaccggc
     1141 cgcagagctg cgcaaccacg caggatcagc cggatgtgtc aatcatcgtt cggctcaata
     1201 ttcggggtca gctcgtcgtc cacgctgagc tcgtcccggt acaggcgctc ccggcagccg
     1261 gggcagaccc gcatctcttc gaccggctcg gtgccggccc gatccccgtc aatcacgatc
     1321 gtggacaact ccgcggccgg ctgaccgcag atgtcgcacg tctccaggcg ctgcccgatc
     1381 gtttcgtgcc gaagcaggct ttcgctctcg aacatcttcc cgctcctttc gggggccgca
     1441 ctgccccata caagcgtaac tgacccgaca cggcctgacc gccgccggct ccccgctttt
     1501 gccccaatcg tagccattga gtcgtggcaa gggcgtccgg cgaccatatc aatctcgtcg
     1561 caagttcgat gcagcgacgc tggcggcagc tgccggcgtc gctagtcgaa cgggatgtgg
     1621 tagtagctgc gcagcttgct gagcttgtgc gtggaattga tgtaccggac ggtgccggag
     1681 gtcgcgcgca tgacgagcgt ctgtgtcgtc gcgccgtcgg agaaatagtg gacgccgctc
     1741 agcagctcgc cgtccgtgat gccggtggcc gcgaagaaga tgttctcgct cgtgacgaga
     1801 tcgttgagcg tgtagatgcg actcaggtcg atatgctgtg ccttcgctac ctgacgttcc
     1861 tcgtcgtttc gtggccaaag ccggcactgc atgtcgccac cgacgcactt gagggcgcag
     1921 gcggcgataa cggcctccgg cgagccaccg atgcccatga gcaagtcaat accagtgccg
     1981 tgcatcgcgg ccatgagggc ggcggcgaca tcaccgtccg tgatcagctt catccgcgcg
     2041 ccggcgtccc gcacctggtt gaccagtgat tcatgacggg gccggtcgag gatgacgacg
     2101 gtcaggtcgt cgatgttgac gcccttggcc ttcgagacgt taacgaggtt ctcctggacg
     2161 gatttggtga tgtcgatcgc gccggcgccc tccgggccga ccgcgatctt ctccatgtag
     2221 gcgatgtcct gccacccgta cagcgagccg cgctcggcga ccgccatgat cacgatcgag
     2281 ctcggcctcc cctgcgcgac cagtcgggta ccctccaccg ggtccaccgc gatgtctacc
     2341 tctggaccct cgccggtacc gacctcctcg cccgtgtaca gcatgggcg
//
LOCUS       DASSGX010000170         2209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1011286, whole genome shotgun sequence.
ACCESSION   DASSGX010000170 DASSGX010000000
VERSION     DASSGX010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011286"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /gene="secY"
                     /locus_tag="VFI12_03045"
     CDS             complement(<1..1171)
                     /gene="secY"
                     /locus_tag="VFI12_03045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HET7055409.1"
                     /translation="MLKVLGNAFAIPEIRRKLAFTAAMLALYRLGSFIPVPGIEADAL
                     NDIQDNFAGSNILGFLNLFSGGSLSRLSLFALGIMPYITASIILQLLTVVVPSLEKLS
                     KEGEVGQQKITQYTRYLTVLLSALQSLGYVFLFKSFGSSGESVVGELTGAKLIMMVAC
                     LTAGAVLVMWLGELVTQHGVGNGISLMIFASIASGIPNGISAWWNNPDQVFVVMMPFI
                     ALAVIVGIVFVQEGQRRIPVQYAKRVVGRKMTTGGSSYLPLRVNMAGVIPVIFAASIM
                     AFPPTVGQLLGGAGDNFWLDFAAFFAPNRWAYVVGEIFFIVIFTYFYTAVTFNPVDQA
                     DNLKKYGGFIPGIRPGRPTAMYLEKVLVRLTVAGALFLAIVAMAPTLFIKYANFDP"
     gene            complement(1175..1666)
                     /gene="rplO"
                     /locus_tag="VFI12_03050"
     CDS             complement(1175..1666)
                     /gene="rplO"
                     /locus_tag="VFI12_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HET7055410.1"
                     /translation="MARIEPEDVGLDNLTPKPGTRKNRKRVGRGHGSGMGKTSGRGHK
                     GQGARAGATRKYGMEGGQNPIHMRMRKLRGPNKKMSMPFEPFRTHTQPVNLANLEERF
                     DAGAEVTPESLKAAGLATRKHPVKILGNGELSKQLTVKAHGFSARAKEKIEAAGGSAE
                     VIE"
     gene            complement(1666..1857)
                     /gene="rpmD"
                     /locus_tag="VFI12_03055"
     CDS             complement(1666..1857)
                     /gene="rpmD"
                     /locus_tag="VFI12_03055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HET7055411.1"
                     /translation="MADLLIKQVRSTAGSSPRQRDTLRTLRLGRIGKSVTRSDDAHTR
                     GLLRKVEHLISVETAGKAS"
     gene            complement(2009..2209)
                     /gene="rpsE"
                     /locus_tag="VFI12_03060"
                     /pseudo
     CDS             complement(2009..2209)
                     /gene="rpsE"
                     /locus_tag="VFI12_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328273.1"
                     /note="located at the back of the 30S subunit body where
                     it stabilizes the conformation of the head with respect to
                     the body; contacts S4 and S8; with S4 and S12 plays a role
                     in translational accuracy; mutations in this gene result
                     in spectinomycin resistance; incomplete; partial in the
                     middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
BASE COUNT          344 a          724 c          750 g          391 t
ORIGIN      
        1 tcgggtcgaa gttcgcgtac ttgatgaaga gcgtcggcgc catggcgacg atcgccagga
       61 acagcgcgcc cgcgacggtc agccgcacta gcaccttctc cagatacatc gccgtcggcc
      121 ggcccggtct gatgccgggg atgaacccgc cgtacttctt gaggttgtcg gcctggtcga
      181 ccgggttgaa cgtgaccgcc gtgtagaagt aggtgaagat cacgatgaag aagatctcgc
      241 cgaccacgta tgcccagcgg ttgggcgcga agaaggccgc gaagtcgagc cagaagttgt
      301 cccccgcccc gccgaggagc tgcccgacgg tcggtgggaa cgccatgatc gacgccgcga
      361 agatgaccgg gatgaccccg gccatgttga cccgcagggg caagtagctc gagccgccgg
      421 tggtcatctt ccggccgacc acgcgcttgg cgtactgcac cggtatccgt ctctgtccct
      481 cctggacgaa gacgatgccg acgatcaccg cgagcgcgat gaacggcatc atcacgacga
      541 agacctggtc ggggttgttc caccaggccg agatcccgtt cgggatcccg gaggcgatcg
      601 aggcgaagat catcagcgag atgccgttgc cgactccgtg ctgggtgacc agctcgccga
      661 gccacatgac cagtacggcg ccggcggtca gacaggcgac catcatgatc agcttcgcgc
      721 ccgtcaactc gccgacgacg ctctcgccgg agctgccgaa cgacttgaag aggaagacat
      781 accccagcga ctgcagggcc gagagcagca cggtcagata gcgcgtgtac tgggtgatct
      841 tctgctggcc gacctcgccc tccttggaga gcttctccag cgacgggacg acgaccgtga
      901 gcagctggag gatgatcgac gcggttatgt agggcatgat cccgagcgcg aacagcgaca
      961 ggcgcgagag gcttccgccg ctgaacaggt tcaggaaccc gaggatgttg gagccggcga
     1021 agttgtcctg gatgtcgttg agcgcgtcgg cctcgatccc gggaacgggg atgaaggagc
     1081 cgagtcggta gagcgcgagc atcgccgccg tgaaggcgag cttgcgacgg atctcgggga
     1141 tcgcaaaggc gtttccgagg accttcagca tcggctattc gatgacttcg gcgcttccgc
     1201 cggccgcctc gatcttctcc ttggccctgg cgctgaagcc atgggccttg acggtgagct
     1261 gcttcgagag ctcgccgttg ccgagaatct tgaccggatg cttgcgggtc gccagacccg
     1321 cggccttgag cgactcggga gtcacctcgg caccggcatc gaaacgctct tcgaggttcg
     1381 ccaggttgac cggctgggtg tgcgtccgga acggctcgaa cggcatcgac atcttcttgt
     1441 tcggaccgcg cagcttgcgc atccgcatgt ggatcgggtt ctggccgccc tccatcccgt
     1501 acttgcgggt ggcgccggcg cgcgcgccct ggcccttgtg accgcgcccg gaggtcttgc
     1561 ccatgccgga gccgtgccca cgaccgacgc gcttgcggtt cttgcgggtc cccggcttcg
     1621 gggtcaggtt gtcgaggccg acgtcctcgg gttcgatccg tgccatcagg aggccttccc
     1681 agccgtctcg acgctgatca gatgctcgac cttgcgcagc agcccgcggg tgtgggcgtc
     1741 atcgctgcgg gtcaccgact tgccgatccg gcccagcctg agcgtgcgga gcgtgtcgcg
     1801 ctggcgcggg ctcgatccgg cggtcgaccg gacctgcttg atcagcagat cagccatcgg
     1861 cgggctcctt cgcgggctcg tccgcggcgg ctgcctcgtc ggcctcgggc ggtgcttcct
     1921 cggtggcgac ggcgaccggg ttgacgacat ttgcggtgtc gtcgtcggcg ccttcggcct
     1981 cgtcggcccg cttgccgtcc ttggggatgc ccaagacctc gcggatcgtc aggccgcgca
     2041 gctcggcgac gtcctcgggc ttgcgcagcc cgcgcaggcc ctccatggtc gccttcacct
     2101 ggttgatcgg gttgttggtg ccgatcgact tcgtcagcac gtcgcggata ccgccgagct
     2161 cgagcacggc gcgcacgccg ccaccggcga tcacgccggt tccctcgct
//
LOCUS       DASSGX010000171         2142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1447772, whole genome shotgun sequence.
ACCESSION   DASSGX010000171 DASSGX010000000
VERSION     DASSGX010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447772"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="VFI12_03065"
     CDS             <1..745
                     /locus_tag="VFI12_03065"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET7055412.1"
                     /translation="EEADRMVAAVDAAGVTCVPFSGMTRLRLADVKARIARGEIGDII
                     LMHQTSRWSIAEDGLNSGRPGWFVDPRHVPGGALIDEGIYWIELFEAFAGPIVRVEAK
                     LGNLVHKDIEVEDWGFATFTFASGVIGTLEASWTINAPRKTGPSPKQNAVVRLEIVGS
                     RGEIIDQWFRSPGRAVLAAGAADWSFERQPEDRFSATSPLVLNHLIDCLEQKQTPETT
                     IHDARRAFGVAIAAYRSARERKAVDVPSV"
     gene            complement(799..2046)
                     /locus_tag="VFI12_03070"
     CDS             complement(799..2046)
                     /locus_tag="VFI12_03070"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HET7055413.1"
                     /translation="MPALDRRDFLKSAGAGLTAATVMLTPREQALAQAEAEKNRLLRI
                     AACSYPIRYIFKNRAGGGRGAGAGRGAGRGAGASGQPGPGPDRAGAPGAGAAAPGAAA
                     APSGTPTPQLPPRSPSNGGWTSQRMKEKYGEITMLDFPQFSKDTFPGVTNMDIFSGLF
                     GDVTDDSMFVGRTFDPMSPSGRKWLDQLANKLVTTGTKVQHISNNAPTNLAGPDETLR
                     KAGVEVAKRWLQGCAVLGVKSMRMNSPQALGPSIRPNAIPRSNDGYPRNLDIIPLLNA
                     AIESYKEMADYGGNLGIRVTFENHWGLAADPMNIRIMLDTINHPYCEASPDFCNWEHE
                     YMLFNGLKALAPYAHTNVHAKYWDRWGDKNDVQRSTRIMLAGGFKGTFALEYEEGPLN
                     GIEGAKYLYKEVLAALTTPTPVV"
BASE COUNT          337 a          693 c          715 g          397 t
ORIGIN      
        1 cgaggaggcc gaccgcatgg tggcagcggt ggatgcggcc ggcgtcacct gcgtgccctt
       61 ctccggcatg acccggctgc gcctcgcgga cgtcaaggcg cgcatcgccc gaggggagat
      121 cggcgacatc atcctcatgc accagaccag ccggtggtcg atcgccgagg acggcctcaa
      181 ctcggggcgg cccgggtggt tcgtcgatcc gcgccacgtc cccggcggcg cgctgatcga
      241 cgagggcatc tactggatcg agctgttcga ggcgtttgcc ggtccgatcg tccgggtcga
      301 ggcgaagctc gggaacctcg tgcacaagga catcgaagtc gaagactggg gtttcgcgac
      361 cttcaccttt gccagcggcg tgatcggaac gctcgaggca tcgtggacga tcaatgcgcc
      421 gcgaaagacc ggcccctcac ccaaacagaa cgcggtcgtg cggctggaga tcgtcggcag
      481 ccgtggggag atcatcgatc agtggttccg ctctccagga cgcgcggtgc tcgctgccgg
      541 ggcggcggac tggtcgttcg agcgtcagcc agaggatcgg ttctcggcga cgtcgccgct
      601 ggtgctcaat cacctgatcg actgtctgga gcagaagcag acgccggaga cgacgatcca
      661 cgacgcccgc cgtgcgtttg gcgtcgcgat cgccgcctac cggtcggcgc gggagaggaa
      721 agcggtcgac gtgccttccg tgtgaggcga agccacgccg acgttcgtgg cctcgcctgc
      781 agcgggacga tcaccctctc aaaccaccgg cgtcggcgtc gtcagcgccg ccagcacttc
      841 cttgtacagg tatttcgccc cctcgatccc gttgagcggc ccttcctcgt actcgagcgc
      901 aaacgtgccc ttgaatccgc ccgcgagcat gatccgcgtc gagcgctgga cgtcgttctt
      961 gtctccccag cgatcccagt atttcgcatg cacgttggtg tgggcatagg gcgcgagcgc
     1021 cttcagcccg ttgaacagca tgtactcgtg ttcccagttg cagaaatcgg gggacgcttc
     1081 gcagtagggg tgattgatag tgtcgagcat gatcctgatg ttcatcgggt cggcggccag
     1141 cccccagtga ttttcgaacg tcacccggat gccgaggttg ccgccgtaat ccgccatctc
     1201 cttgtacgac tcgatcgcgg cgttgaggag cgggatgatg tcgaggttgc gcggatagcc
     1261 gtcgttggag cgcgggatcg cgttcggccg gatgctcggt ccgagcgcct gcggagagtt
     1321 catccgcatc gacttgacgc caagcacggc acatccctga agccagcgtt tggccacttc
     1381 cacgcccgcc ttccggaggg tttcatccgg accggcaaga ttggtcggcg cgttgttgga
     1441 gatgtgctga accttggtgc cggtcgtcac caatttgttg gcgagctgat cgagccactt
     1501 ccgcccggat gggctcatcg gatcgaaggt gcggccgacg aacatcgagt cgtcggtgac
     1561 gtcgccgaac agccccgaga agatgtccat gttggtcacg cccgggaacg tatccttgct
     1621 gaactggggg aagtccagca tcgtgatctc gccgtacttc tccttcatcc gctgcgacgt
     1681 ccagccgccg ttggaaggcg aacgcggcgg caattgcggc gtcggcgttc cggacggcgc
     1741 ggcggcggcg cccggagcgg ccgccccggc gccgggtgcg ccggcacggt cgggtcccgg
     1801 cccgggctga cccgatgcgc ccgcaccgcg accggcgcct cggccagcac ccgccccacg
     1861 gccgccgcca gcgcggttct tgaagatgta gcggatcggg tacgaacagg cggcgatccg
     1921 gagcagccgg tttttctccg cctcggcttg cgccagcgcc tgttcgcgcg gggtcaacat
     1981 tacggtggcg gccgtcaatc cggcgccggc gcttttcaga aagtctcgtc tatcgagtgc
     2041 aggcattggt cctctccact tatcgtttgc tcaaatccct gatgatgatg tccttgaaca
     2101 gcatgttgcc ctctccgccc gagtgcaact ggagggcgag cg
//
LOCUS       DASSGX010000172         2199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_903925, whole genome shotgun sequence.
ACCESSION   DASSGX010000172 DASSGX010000000
VERSION     DASSGX010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903925"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..1597)
                     /locus_tag="VFI12_03075"
     CDS             complement(17..1597)
                     /locus_tag="VFI12_03075"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7055414.1"
                     /translation="MASVVKQPATYDAIVIGAGHNGLVAACYLAGAGLNVCLLERYHE
                     VGGAAISEETIPGFKLSTGSYVLSLAPRKIFDELGAWEGIELLDRNPRFFAPFPDGSS
                     LTYWNNHEKWLDSIRKVNPKDADAYDVYDDAVEVASGVMDKYILRRPPSWDEVASNFR
                     TPEEQKIFQMFYLGAVADIAEYYFDSDQMQATVSSSGIIGTFRGPRDPGTGYVKLYHS
                     MGMSTGHRGRWAYVRGAMGAITQALKKVAERRGVTIRTNANVEQIIVKNGRATGVLLD
                     SGEEIASDLVLSNADPKRTYLGLVEERDVPAAYRRSISNIKIESPVIKINMAVRELPK
                     FLALPDDQQLQGSTGGLFIAPSIDYIQKAYYDAKAGKPAEQPFMNIHMQSAVDRTVAP
                     GDMHTISIFTQYFPYTLAEGTWDERRDEIAQNVIREFAKFAPNIPDAIVGMQVLTPPD
                     IESRFGMTGGHIFHGELVPEQAFDMRPVPGSSSYEGPIGGLFLCGSGAWPGGCVMGAP
                     GHNAAHEAIGKLRAGRHG"
     gene            complement(1742..1924)
                     /gene="rpsU"
                     /locus_tag="VFI12_03080"
     CDS             complement(1742..1924)
                     /gene="rpsU"
                     /locus_tag="VFI12_03080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HET7055415.1"
                     /translation="MRVEIKDSESFDQLLRRFQKGIERSGIIREHRRGLRFISTQEEN
                     RAKRRKAERRRRRNQA"
BASE COUNT          382 a          698 c          698 g          421 t
ORIGIN      
        1 gtataggaac gccgccctac ccgtgccggc ccgcccgcaa cttgccgatg gcctcgtggg
       61 cggcgttgtg gcccggcgcg cccatcacgc agccgcccgg ccaggcgccg gaaccgcaga
      121 ggaacaggcc gccgatcggc ccctcgtagg acgaactacc cgggaccggc cgcatgtcaa
      181 aggcctgctc cggcaccagc tcgccgtgaa agatgtggcc accagtcatg ccgaagcgcg
      241 actcgatgtc cggcggcgtc agcacctgca tcccgacaat cgcgtccgga atgttcggcg
      301 cgaacttcgc gaactccctg atcacgttct gcgcgatctc gtcccgccgc tcgtcccagg
      361 tgccctcggc cagggtgtag gggaaatatt gcgtgaagat cgagatcgtg tgcatgtcgc
      421 ccggcgcgac cgtccggtcg acggccgact gcatatggat gttcatgaac ggctgctcgg
      481 ccggtttgcc cgccttggcg tcgtagtagg ccttctggat gtaatcgatg ctcggcgcga
      541 tgaagaggcc gccggttgag ccctgcagct gctggtcgtc cggcagcgcc aggaacttcg
      601 gcagctcgcg cacggccatg ttgatcttga tgaccggact ctcgatcttg atgttgctga
      661 tcgaccgccg gtaggcggcc ggcacgtccc gctcctcaac cagaccgagg tacgtccgct
      721 tcgggtcggc attcgagagg acgagatcgg acgcgatctc ctcacccgaa tcgagcagca
      781 cacctgtcgc gcggccattc ttgacgataa tttgctccac gttcgcattc gtgcgaatcg
      841 tgactccccg gcgctcggcg accttcttga gcgcctgcgt gatcgcgccc atcgcgcccc
      901 gcacataggc ccagcggccg cgatgacccg tggacatgcc catcgagtgg tagagcttga
      961 cgtagccggt accgggatcg cgcggaccgc ggaaagtgcc gatgatgccg ctggagctga
     1021 ccgtcgcctg catttggtcg ctgtcgaagt agtactcggc aatatcggcg acggcgccga
     1081 ggtagaacat ctggaagatc ttctgctctt caggcgtgcg gaagttgctg gcgacttcgt
     1141 cccagctcgg tgggcggcgc aggatgtatt tgtccatcac gccgctggcc acttcgacgg
     1201 cgtcgtcgta gacgtcgtag gcgtcggcat ctttcgggtt gaccttgcga atgctgtcca
     1261 gccacttttc gtggttgttc cagtaggtca gcgaactgcc gtccgggaag ggcgcgaaaa
     1321 agcgagggtt gcgatcgagc agctcgattc cttcccaggc gccgagctca tcgaagatct
     1381 tgcggggcgc gagcgagagg acatacgagc cggtcgagag tttgaaaccg gggatcgtct
     1441 cctcggagat tgccgcgcca ccgacctcgt ggtaacgctc gagaaggcag acgttgaggc
     1501 ccgcgccggc caggtagcaa gcggcaacga gaccgttatg tcccgcgccg atgacgattg
     1561 cgtcatacgt tgcgggctgt ttgacaaccg acgccatgaa ttggtcctcc gagccgctga
     1621 gcgacgtgtt ctgtgttgga tcgcgaacgc gtcggtagga tagcaccggt tcccgtacgc
     1681 aaatgagccg gacgggaagt ccggctcaag gcagtcagat tggagatgtg aagggcgtcg
     1741 gctaggcctg gttgcggcgt cggcggcgct ccgccttgcg gcgcttggcg cgattctctt
     1801 cctgcgtgct gatgaagcgc agtccgcgac gatgctcgcg aatgatcccc gaccgctcga
     1861 tgcctttctg gaatcgtcga agcagttggt cgaacgactc cgaatccttg atctcaaccc
     1921 gcatgcgtta cattcaccac ctgtgatgga aagcggcgtc gatgacgcgc accaaaaacc
     1981 tgtccgatcc tgtcggccat ctgaaagtat acgataccgg tcaatagttt cgggagaatg
     2041 tatgtgtcca cccccacact cgtcccgggc ctcgcggtca ccccgggcac gccgcattcg
     2101 cttcacctga ccgagatcgc gctgcctgac gtcggccccg gcgacgtgca gatccgcgtc
     2161 cttcaggccg gcatctgcgg gaccgaccgg gagatcatc
//
LOCUS       DASSGX010000173         3870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_250151, whole genome shotgun sequence.
ACCESSION   DASSGX010000173 DASSGX010000000
VERSION     DASSGX010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3870
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250151"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..1217)
                     /locus_tag="VFI12_03085"
     CDS             complement(183..1217)
                     /locus_tag="VFI12_03085"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HET7055416.1"
                     /translation="MAVDTRQYAGQATNGGSDPLLKVSNLVMHFPLTQGIIFQRKVGA
                     VQAVDGISFEVKKGETLGLVGESGCGKSTTGRAILQLYKPTSGEIVFRGKDLTKLPGG
                     DMRKMRRHLQMIFQDPYASLNPRMTVGSIIAEPMQIHGLVEKKDRNKRVQDLLQTVGL
                     NPYFANRYPHEFSGGQRQRIGIARALAANPDFIVADEPVSALDVSIQAQIVNLLEDLQ
                     DQFGLTYLFIAHDLSVVKHISDRVAVMYLGKIVEMASRNDLYDQPLHPYTKALLSAVP
                     IPDPVVERRRERIILSGDVPSPINPPSGCHFHTRCPYVMDVCKQIDPIFADQGNGHFV
                     ACHLYPGSGK"
     gene            complement(1220..2245)
                     /locus_tag="VFI12_03090"
     CDS             complement(1220..2245)
                     /locus_tag="VFI12_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7055417.1"
                     /translation="MKTFRDGQPLLEVKDLRTQFMTQDGVVKAVDGVSFYVMPGETLG
                     VVGESGSGKSMTGLSIMRLIPIPPGKIASGEVLLEGRDVLKMSDNDVRKIRGKEVAMI
                     FQDPMTSLNPVLTISRQLGEAIQLHLGMSKEQARARSVDLLNMVGIPNAEKRLDQYPH
                     QFSGGMRQRVMIAMALSCNPKLIIADEPTTALDVTIQAQILDLMRTLQSERDTGVILI
                     THSMGVVAGMADRVQVMYAGHIVETASTEEIFANPRHPYTVGLMKSIPRLDATTKEKL
                     EPIRGLPPDLIDLPDMCPFVPRCNYAREKCEQKNPPLLEVAPGHFSACWFWDEVSMEN
                     EPPRRDA"
     gene            2509..3591
                     /gene="dprA"
                     /locus_tag="VFI12_03095"
     CDS             2509..3591
                     /gene="dprA"
                     /locus_tag="VFI12_03095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HET7055418.1"
                     /translation="MPSVDARFWVGFQHVRRIGPVRLKRLIDRFGSISDAWVAPQSEL
                     RTVLDEASVESIARVRASLDLDREMERIERLGIDVLTLADAAYPALLREVPNPPAVLY
                     LKGAIVDADVKAVGMVGTRRCTGYGRQVAASMAEDFARAGVTVVSGLALGIDGQAHRG
                     ALAGGGRTIAVLGSGVDLVYPSSHRDMAAQIVENGAIISDYSPGTKPDARNFPPRNRI
                     IAGLSRGVVVVEAPVRSGALITVDFAADYGRDVFCVPGNVQSELSAGCHAVIRDGGRL
                     VTSAADVLEDLGMTVPAPGTAVQQTFPMSDEERHLVNHIRWDPQHIDEIAVSAGFTSP
                     QASALLTLLELKGAIRDAGGQHYVRC"
BASE COUNT          695 a         1172 c         1259 g          744 t
ORIGIN      
        1 atgcatgaat tcatgcaccg tgatcgcgat gacgccggag agcagatagg ccacgatctg
       61 gtcggtcggc gggatcctcc aaccgctgct gaggagattc ggcagcacga aaatgagcag
      121 gccgaggatg aagagttgtc tcggatccca gcctgctcgc acggtgtggc ctcagtagtt
      181 ggttacttgc cggaccccgg gtagaggtgg caggcgacaa agtgcccgtt gccctgatcc
      241 gcgaagatcg gatcgatctg cttgcagaca tccatgacgt acgggcaacg agtgtggaag
      301 tgacaaccgg atggcggatt gatcgggctc ggcacgtcac cgctgaggat aatccgttca
      361 cggcgcctct cgacgaccgg gtccgggatc ggaaccgcgg aaagcagcgc cttggtgtac
      421 ggatgcagcg gctgatcgta gagatcattc cggctggcca tctcaacgat cttgccgagg
      481 tacatcaccg cgacgcgatc agagatgtgt ttcacgaccg acaagtcgtg cgcgatgaac
      541 aggtaggtca ggccgaactg gtcctgcagg tcttcgagca ggttgacgat ttgcgcctgg
      601 atcgacacgt cgagcgcgga aaccggctcg tccgcaacga tgaagtcggg gttcgccgcc
      661 aacgcccggg cgataccaat gcgctgacgc tggccgccgg agaactcgtg cggatagcgg
      721 ttcgcgaagt acgggttgag accgaccgtt tgcagcaagt cctggactcg cttgttgcgg
      781 tccttctttt cgaccagccc gtgaatctgc atcggctcgg cgatgatgct gccgacggtc
      841 atgcgcgggt tcagtgacgc atacgggtcc tggaagatca tctgcaggtg gcgtcgcatc
      901 ttgcgcatgt cgccgcccgg cagtttggtc aggtccttgc cacggaagac gatttcgccg
      961 ctcgtcggct tgtacagctg aaggattgcg cgcccggtcg tggacttgcc acagccggat
     1021 tcacccacga ggccgagggt ctcgcccttc ttgacctcga acgagatgcc gtcgaccgcc
     1081 tgcacggcgc cgaccttgcg ctggaaaatg atgccctgcg tcagtgggaa gtgcatgacc
     1141 aggttgctga ccttgagcag ggggtcgctg cctccgttgg ttgcctgccc ggcgtattgg
     1201 cgagtgtcca cggccattgc taggcgtccc tccggggcgg ttcattttcc atgctgacct
     1261 cgtcccagaa ccagcaggct gagaaatggc cgggtgcgac ttcgaggagc ggcgggttct
     1321 tctgctcgca cttctcgcgc gcatagttgc agcgcgggac gaacgggcac atgtccggta
     1381 gatcgatcag gtccggcgga aggccacgga tcggctcgag cttctccttg gtggtcgcat
     1441 ccagccgcgg gatcgacttc atcaggccga ccgtgtacgg gtggcgcgga ttggcaaaga
     1501 tctcttcggt gctcgccgtc tcaacgatgt gccccgcgta catgacctgg acgcgatcgg
     1561 ccattccggc cacgacgccc atcgagtggg tgatcaggat cacgccggtg tcacgctcgc
     1621 tctgaagtgt ccgcatcagg tcaaggattt gtgcctggat tgtcacgtcg agcgccgtgg
     1681 tcggctcgtc ggcgatgatc agcttcgggt tgcatgagag cgccatggcg atcatcacgc
     1741 gctggcgcat gccgccagag aactggtgtg ggtactggtc gagtcgtttc tcggcattcg
     1801 gaataccgac catattgagc agatcgaccg agcgggcccg ggcctgctct ttcgacatgc
     1861 cgaggtggag ctggatcgcc tcgccgagtt gccggctgat cgtgagcacg ggattgagcg
     1921 aggtcatcgg gtcctggaag atcatggcaa cttccttgcc acgaatcttg cggacatcgt
     1981 tgtcggacat cttgaggacg tctcgaccct cgagcaacac ttcgccactg gcgatcttgc
     2041 ccggcgggat cggaatcaga cgcatgatcg agagaccggt catgctcttt ccggagcccg
     2101 attcgcccac gacgccgagc gtctcacccg gcatcacata aaaggaaacc ccgtcaactg
     2161 ccttgacgac gccatcctgc gtcatgaact gggttcgaag gtctttgact tcgagaaggg
     2221 gctgaccatc tcgaaatgtt ttcacccggg acgctggtgc tgtttctgcg accatcgttc
     2281 atcaatcctt aaaagcgcgt tggacggtct cgtccctgtt tcgccgccag tcgtcgccga
     2341 tcgcagcggg tatcggattg ctcagcaaag cgtccacgcg aatacacagg cgcgcttcgt
     2401 tggaatcgtg atcggcagga tagcatacgg taggagacgc aaccaagcat gtctcaccgc
     2461 ctgcgcgccg ggcgcgggtg gtgtcttacg aatactcagg tgccagcaat gccatcggtc
     2521 gatgcccgct tttgggtagg ttttcagcac gtccggcgga ttgggccggt ccggctcaaa
     2581 cggctgatcg atcggttcgg atcgatcagc gatgcatggg tagctccgca atccgagctc
     2641 cggactgtgc tcgacgaagc atcggtcgag agcatcgcgc gggtgcgggc ctcgctcgat
     2701 ctcgaccggg agatggagcg aatcgagcgc ctgggcatcg acgtgctgac gctcgccgac
     2761 gcggcgtatc cggccctttt gcgtgaggtg ccgaacccgc cggccgtgct ttacctcaag
     2821 ggagcgattg tcgatgccga cgtcaaggcg gtcggcatgg tcgggacacg ccgatgcacg
     2881 ggttatgggc ggcaggtggc ggcgtcgatg gccgaggatt tcgcccgggc cggcgtgacg
     2941 gtcgtctcgg gcctcgcgct cgggatcgac ggccaggcgc accggggggc gctcgccggc
     3001 ggtggccgga cgatcgcggt gctcggctcg ggcgtggatc tggtctaccc gtccagccat
     3061 cgcgacatgg ccgcgcagat cgtcgaaaac ggcgcgatca tctcggatta ctctccgggc
     3121 accaagcccg atgcccgcaa cttcccaccg cgcaaccgga tcatcgccgg attgagccgc
     3181 ggcgtggtcg tggtcgaggc gcccgtccgc agtggcgcgc tgatcacggt cgatttcgcc
     3241 gctgactacg gccgggacgt cttctgcgtg ccgggcaacg tccagagtga gctgagcgcc
     3301 gggtgccatg ccgtgattcg cgacggcggc cggctggtga caagcgcggc ggacgtgctc
     3361 gaagatctcg gcatgaccgt cccggcgccg ggcacggcgg tccagcagac cttcccgatg
     3421 tcggacgagg agcgccacct cgtcaaccac attcgctggg atccccagca catcgacgag
     3481 atcgccgtct cggccgggtt tacatcgcct caggccagtg ccctgttgac gttgctcgag
     3541 ctgaagggcg ccatccggga cgccggcggc cagcactacg tccggtgttg atgcctcgtc
     3601 agttggcttg acagcccttg acaccatccg tacggttgaa aagatatcgg cgcccccatc
     3661 accagcgccg atccttttcg ttccgacgca tgaatatatt gaatagagac cttagatatg
     3721 ccgacatcca aaaccggtgg ttccggcacg cgagcaacca cgaaacccgc gacaaagaag
     3781 aagacgacgg cgagcaaggc gcgcaaatcg aacggccgcc ttgtcatcgt cgagtcgccg
     3841 gccaaggcgc gcacgatcga gcgatacctc
//
LOCUS       DASSGX010000174         6286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1012077, whole genome shotgun sequence.
ACCESSION   DASSGX010000174 DASSGX010000000
VERSION     DASSGX010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6286
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012077"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1790
                     /locus_tag="VFI12_03100"
     CDS             <1..1790
                     /locus_tag="VFI12_03100"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET7055419.1"
                     /translation="VPPLDVPAAGSGCLAEHAAVRLFIERAADLSPNLTPETLDAIVA
                     ICHRLDGLPLAIELAAAWTGVLPPAQLLDRLSARLPMLTGGTRDMPARHQTMRDSIAW
                     SYDLLAPDEQMVFRRAGVFAGGFCLEAIEEVATGAGGRAEVLGHVRSLAEKSLIRPVP
                     VAGVPRYQMLETIREFALAQLIEEGEEEIARGMHANWCAEFAERFGAEVTGPDLATWT
                     EWAEAELDNVRSALSHAEARGDGATAVRLAGGIGWLWMYPGRLHEGRAALARARAMSG
                     ADAVPKAFVSALMCAGGIENWLEDFAAGRHLYERALTVCQAEGDDYLAAIAIRGIGAA
                     SIYTGELRQARDLLTMSRQWAIEARLAWDVAFATHLIGVVSIANGDLVEARSCQEEAL
                     AAFRQMGDREYTANVLEAIGWVALLTGDQESARAAFLEMLGLSAANDRWCLARALRGL
                     GAVAEDRDPRRAARLLAVAEREFAAIGTWHRVPVEASYIEIRERIRASLGKEAYAEEW
                     ALGAGMPLDEAVAEARIVTVKRVDPIAVRFGLTRRETEVLRLITDGLTDQQIADRLFI
                     SRRTASKHVESILAKLNVDSRRAAASLLA"
     gene            complement(2036..2545)
                     /locus_tag="VFI12_03105"
     CDS             complement(2036..2545)
                     /locus_tag="VFI12_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055420.1"
                     /translation="MRQLIIALILLGSVLALGPAQSATAAPAVTYYVMPTVSVTPTLC
                     SGVECDTFGIRYDYSGEGSCTSCYPTDLLRAGLFSLTFTPNRFFPNDPIRARSGIGTL
                     SVVWADSTTTTAAYSFKARDSKAYSLTGKVTGGTNSRFTAGTAVDGLVGLPPNPVEPG
                     TTAAAISFG"
     gene            complement(2820..3581)
                     /locus_tag="VFI12_03110"
     CDS             complement(2820..3581)
                     /locus_tag="VFI12_03110"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7055421.1"
                     /translation="MTVRSDAEHDDRPARLADMNSSESPGDGKWLSIHEACRLLGVDQ
                     STLRRWSDSGKVPVFRTPGGHRRYAESELLALVGNGSRRFERQRVMARQLADQSRAGF
                     VDEFWRTARTRRWYRAYSPNQLEDLRRLGRRLIDLAVRYASTAAGPERQSLIDEGRSI
                     GHQYARVGAMAGLSGAEAVEAFLYFRAPVVHSLTSLTEGSRMGAPAMLRVSAEVNSFL
                     DQVLLAMVHEHEEQSRLTAGATIQPGIEGQFLDDL"
     gene            3861..5144
                     /locus_tag="VFI12_03115"
     CDS             3861..5144
                     /locus_tag="VFI12_03115"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HET7055422.1"
                     /translation="MGETTDVGVAELLSVLARRGHTGRLHINAQGEEVQVVLNGGKVT
                     QVSSSHHSLRIGRVLVRMGALKEADLNEAVKEQVSQQTSKPLGQILTSFGLVTQGDLA
                     RAAEEQATDALSRVFGAQSGTFYFTGLAAENARPGLMALNAEGIVLEASRRADEIEAL
                     RQMMPADEAVLTLDRSQIPIGDRLPEHELRVVRVLGMEPMTLPELVAELADDERAVLR
                     AIVGLRERGIVAIRHSSQPGGSEAVEELTIAPRRAAELRELIGADHKPGSNAGIPGIA
                     EIRGATPAGTQTVARATRVVREVVGAFNAGLPLLAFAHFTDAYFRRLAPISDEAFAQF
                     ERLAMPDDDEHQTFIDLRDVRALADGRISGIAVTSLADDEPTQKVVVFAELGDRVLID
                     AIIEPGKDRARQTQTTLLHPTTLFGADRASLRRTL"
     gene            complement(5433..6233)
                     /locus_tag="VFI12_03120"
     CDS             complement(5433..6233)
                     /locus_tag="VFI12_03120"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET7055423.1"
                     /translation="MSQRVEWNAEHYHVVSRPHEVWGARVLDRVPHEGIRVAIDAGCG
                     TGKITRELLDRLPEATIYALDVSPQMLAVAERELAEYGDRVRFRQTDLGSISPEDVGE
                     TADLIFSTATFHWVKDHDRLFRNLFALLKPGGWLIAQCGGGPNIATLRRRMAALMKEP
                     RFAPHFAGWADPWYFATAEETADRLHAAGFGDVQTNVEPHPAPMADADEYRSFITTVV
                     IRDQLARLPDQELKDAFANALTDLAATDTPPYELDYHRLNIHARRSMG"
BASE COUNT         1014 a         1952 c         2252 g         1068 t
ORIGIN      
        1 ccgtgcctcc gctcgacgtg ccggcggcgg gcagtggttg cctcgcggag catgcggcgg
       61 tacgcctctt cattgagcgc gcagcagacc tgtcgccgaa cctgaccccg gaaacgcttg
      121 acgccattgt cgcgatctgt caccggctcg atgggttgcc gctggctatc gagcttgcgg
      181 cggcgtggac cggcgtcttg cccccggcgc agctgctcga ccggctgagc gcgcgcttgc
      241 caatgttgac cggtggcacg cgggacatgc cggcgcggca tcagacgatg cgcgactcga
      301 tcgcctggag ctacgacctt ctcgctccgg acgagcagat ggtgttccgg cgggcgggcg
      361 ttttcgccgg cggattttgc ctggaggcga tcgaggaggt cgcgacgggc gcaggcgggc
      421 gggcggaagt gctcggccac gtgcgatcgc tggccgagaa gagcctgatc cggccggttc
      481 cggttgccgg cgtgccgcgc taccagatgc tcgagacgat ccgtgaattc gccctggcgc
      541 aactaatcga ggagggtgag gaggagatcg cccggggtat gcatgccaat tggtgcgctg
      601 aatttgccga gcgattcggc gctgaggtga ctggtccgga cctggcgacg tggaccgaat
      661 gggctgaggc cgagctggac aatgtgcggt cggcgctcag ccatgcggag gcgcggggag
      721 acggtgcaac cgccgttcgc ctggccggcg gaatcggctg gctctggatg tatcccgggc
      781 ggttgcacga aggacgggcg gcactggccc gggcacgagc gatgtccggc gcggatgcag
      841 tcccgaaggc atttgtgtcg gcgctgatgt gcgccggcgg aatcgagaat tggctggagg
      901 atttcgccgc cggtcgacat ctctatgagc gcgccctgac ggtttgccaa gccgaaggcg
      961 acgactacct ggccgcgatc gcgatacgcg ggattggcgc ggcatcgatc tacactgggg
     1021 agctgcgaca agcccgcgat ttgctcacca tgtcccggca gtgggcgatc gaggcacgat
     1081 tggcctggga cgtggccttc gcgacgcatc tcattggggt cgtctcgatc gctaacgggg
     1141 accttgtcga ggcgcgttcc tgccaggagg aggcgctcgc ggcgttccgg cagatgggcg
     1201 atcgcgaata cacggccaac gtgctcgagg cgatcggctg ggttgcgttg ctgactggcg
     1261 atcaggaatc ggcgcgcgcc gccttcctcg aaatgctcgg gttgagcgcg gcgaacgatc
     1321 gctggtgcct ggcgagggcg ctgcgcggac tcggcgcggt ggcagaggac cgtgatccgc
     1381 gacgcgcggc ccggttgctt gccgtggcgg agcgggagtt tgccgcaatc ggcacatggc
     1441 atcgcgtccc cgtcgaggcg agctacatcg agatccggga gcgcatccgg gcatccctcg
     1501 gcaaggaggc ctatgccgag gagtgggctc tgggagcagg gatgccgctt gatgaggccg
     1561 tggctgaggc caggatcgtc acggtcaagc gcgtcgaccc gattgcggtt aggttcggac
     1621 tcacgcggcg ggagaccgag gtattgcggt taattacgga cgggctgacc gaccagcaga
     1681 tcgcggaccg cctcttcatc tcccgccgca ccgcctccaa gcacgtcgaa tcgatcctgg
     1741 ccaagctcaa cgtcgattcg cgccgcgccg ccgcctctct attggcgtag cgcggtcccc
     1801 tgcgggccgc ccgggccccg gccccacgcg atcgttggcg gcaaccaacc ccggcccact
     1861 aaccggcttc aacggacagg cccggggagg caggatgcgg cccacggggg gaccgcgcta
     1921 cgccattggt tggtgggatg agggaccccg acggcgccga gggtggcgcc gggggcggtg
     1981 caggagtggg ccggccgggc gcgtgcctgc gcggttccgg gccggggtga agtgactagc
     2041 cgaagctgat ggcagcggcg gtcgtgccgg gctcgactgg attgggtggg aggccgacca
     2101 gtccgtcgac ggcggtgccg gcggtgaagc gggagttggt cccgccggtg accttgccgg
     2161 tcagcgagta ggccttggag tctcgcgcct tgaacgagta ggcggcggtt gtggtcgtgc
     2221 tgtcggccca gaccacgctc agcgtgccga tcccgctcct ggccctgatc gggtcattgg
     2281 gaaagaaccg gttgggtgta aacgtgagcg agaacaaacc ggcgcgtaag agatcagtgg
     2341 ggtagcagct ggtacagctg ccctcaccgc tgtagtcgta cctgatgccg aaggtatcgc
     2401 actcaacgcc gctgcagagg gtcggggtca cgctcaccgt cggcatcacg tagtaggtca
     2461 cggccggggc ggccgtcgcc gattgcgcgg ggccaagagc cagcacgctc ccaagcagga
     2521 tcagggcaat gatcagctgt ctcatgacgc attctcctcc gtaccctcgg cagggtcgct
     2581 cacccatttc ggcgcgattg acgcagatca tgcccagcgc aattgccgcg cgcatgggga
     2641 gaatgtacgt agttttggag cggggtggta cgtatgtttc ggggacgtcg gacgtcggac
     2701 gtcagttgag ggatcaggga tcaggcaacc atggcgggca cagcacgctg cgtgcgacgc
     2761 tcattcctga agtctgaagt ctgaagtctg aagtctgaag tctgaagtct gaagtccgct
     2821 tacaggtcgt ccaggaactg gccttcgatg ccgggttgga tcgtcgcgcc ggcggtgaga
     2881 cgggattgct cctcgtgctc gtggaccatc gccagcagga cctggtcgag gaagctgttg
     2941 acctcggccg agacgcggag catggccggc gcgcccatgc gggagccctc ggtcagcgac
     3001 gtcagggagt ggaccaccgg ggcgcggaag tagaggaacg cctcgacagc ctcagccccg
     3061 ctcaaaccgg ccattgcccc gacgcgggcg tactggtgcc cgatcgagcg gccctcgtcg
     3121 atcagcgact ggcgctccgg tccggcggcg gtggaggcgt agcggacggc gagatcgatc
     3181 aggcggcggc cgagccggcg caaatcctcg agctggttgg gggaataggc gcggtaccag
     3241 cggcgggtgc gtgccgtccg ccagaactcg tcgacgaagc cggcgcggga ttgatcagcc
     3301 agttgcctgg ccatcacccg ctgccgctcg aagcggcgag agccgttgcc gaccagcgcc
     3361 agcagctccg attcggcata tcggcggtgt ccgcccggcg tccggaagac cgggaccttg
     3421 cccgaatcgc tccagcggcg cagggtggac tggtccacgc cgagcaatcg acaggcttca
     3481 tggatggaaa gccatttgcc gtcgcccggg gactcggagc tgttcatgtc cgcgagtcgg
     3541 gcgggccggt cgtcgtgctc agcgtcggaa cgcacggtca ttctttctcc cccaaggtgt
     3601 gtcgggcaca gtgggcgcgg ccgcgagtaa cgaggccgac atcagcgcac cgttgagatt
     3661 caaagtctac cctcccggga caaagtgtca acgccggtca tgtcacatcg gcccatgcgc
     3721 aaatgtgcgc aattcgtgca cttaggaccg ccggggcatg gcttttgcgc aacttcggcg
     3781 cgatgttatg gagagcggag ttccggaacc acctgctgcg cgcacgtttt cacatcaggg
     3841 tgcttattta catggcgttc atgggcgaaa caacggatgt cggcgttgcg gagctgctct
     3901 ccgtcctcgc gcggcgaggg cataccggtc gattgcacat caatgcccag ggcgaagagg
     3961 tccaggtcgt tctgaacggc gggaaggtta cccaggtctc ctcctcacat catagcctcc
     4021 ggatcggccg ggtcctggtg cggatgggcg cgctcaagga agcggacctc aatgaggcgg
     4081 tcaaggaaca ggtcagccag cagacgagca agccactcgg ccagatcctg acctcgttcg
     4141 ggctggttac gcagggcgat ctcgccaggg cggccgagga gcaggcgacc gacgcgctct
     4201 cccgcgtctt cggcgcgcag tcaggcacct tttatttcac cgggcttgcc gccgagaacg
     4261 cacggccggg gctgatggcg ctcaatgccg aagggatcgt cctcgaggcc tcccgccggg
     4321 ccgacgaaat cgaggcgctc cggcaaatga tgccggccga cgaggctgtc ctgacgctgg
     4381 atcgttcgca gattcccatc ggcgaccgct tgccggagca cgagctgcgc gtggtgcgcg
     4441 tgctcggcat ggagccgatg acgctcccgg agctcgttgc cgaattagcc gacgatgagc
     4501 gagcggtcct gcgcgcaatc gtggggctcc gagagcgcgg aattgtcgca attcgacatt
     4561 ccagccagcc cggcgggtcc gaggccgtgg aagagctgac gattgcgccg cgccgggccg
     4621 ccgaattgcg tgaattgatc ggcgccgatc acaagcccgg gtcgaatgcc ggaatcccgg
     4681 gtattgccga gatccggggg gcgactccgg ccggaaccca gaccgtggcc cgggcgacgc
     4741 gggtcgtgcg ggaggtcgtc ggcgcgttca acgcggggct gccgctgctt gccttcgccc
     4801 atttcaccga tgcgtatttc cgtcggctgg ccccgatctc cgatgaagcg ttcgcgcagt
     4861 tcgagcggtt ggccatgccg gatgacgacg agcatcagac cttcatcgat ctgcgcgacg
     4921 tgcgggcgtt ggccgacggc cgaatcagcg gcatcgccgt gaccagcctc gccgacgatg
     4981 agcccacgca gaaggttgtc gtgttcgccg agctcggaga ccgggttctg atcgacgcga
     5041 tcatcgagcc gggcaaggat cgcgcgcggc agacccagac gacgctcttg cacccgacca
     5101 ccctcttcgg cgccgaccgc gcgtcccttc gccgcaccct gtgacgcggg ttaagggtta
     5161 agggaacgaa acggcgtagc cccgttcccc gtagccggcc tcatgcccca agctcggcgt
     5221 ggcgcggtcc cccatggggc ccaatcccca tcccgacgta agcgttccgc ggaacgaacc
     5281 gccgtgggag ggatcggcct catggactgc gcggggggag gcagggtgcc gaccatgggg
     5341 gaccgcgcta cgctggggca tttgaacggg ccggtcagga atgacgggca gatgccatct
     5401 gcccctacat catttccctc aaccctcaac tctcaaccca ttgaccggcg cgcgtggatg
     5461 ttcagacggt ggtagtcgag ctcgtagggc ggggtgtcgg tagcggcgag gtcggtgagg
     5521 gcattggcga aggcgtcctt cagttcctga tcgggcaggc gggcgagctg atcacgaatc
     5581 acgaccgtgg tgatgaagga gcggtattcg tcggcatctg ccattggcgc cgggtgcggc
     5641 tcgacattgg tctggacgtc gccgaagccg gcggcgtgga gtcgatctgc cgtttcctcg
     5701 gccgtggcga agtaccacgg gtcggcccag ccggcaaagt gcggggcgaa tcgcggctcc
     5761 ttcatcagtg ctgccatgcg ccggcggagg gtcgcgatgt tcggcccgcc gccgcattgc
     5821 gcgatcagcc aaccaccggg tttgagcagg gcgaagaggt tgcggaagag ccgatcgtgg
     5881 tccttcaccc agtggaacgt ggccgtgctg aagatgaggt ccgccgtctc gccgacgtcc
     5941 tcgggactga tcgagccaag gtccgtttgc cggaaccgga cgcggtcgcc gtactcggcc
     6001 agttcccgct cggcgacggc cagcatctga ggcgagacgt cgagggcgta gatggtcgcc
     6061 tctggcaggc ggtcgagtag ctcccgggtg atcttgccgg ttccgcagcc ggcgtcgatc
     6121 gcgacccgga tgccctcgtg cggtacacgg tcgagcacgc gcgctcccca gacctcgtgc
     6181 ggccgggaga caacgtggta gtgctcggcg ttccattcga ctcgctgcga catgcgtccc
     6241 tcctccggat gctccggtat cctacccgga tgatgcttca ccttcc
//
LOCUS       DASSGX010000175         3430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_359893, whole genome shotgun sequence.
ACCESSION   DASSGX010000175 DASSGX010000000
VERSION     DASSGX010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359893"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VFI12_03125"
     CDS             <1..449
                     /locus_tag="VFI12_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055424.1"
                     /translation="TRVQEALVTVGIPVLRNQTTVVRYGGTQLAIAGIDETLLGDPEP
                     AATIAQAPPGVPVLALWHEPEFAEQAAAAGAFAQLSGHTHGGQIRLPGIGPVGLPVHG
                     HRHIIGEGRADGMPIYTSRGVGVYRPPARFNCPPEVTLVTLVAPTT"
     gene            533..1720
                     /locus_tag="VFI12_03130"
     CDS             533..1720
                     /locus_tag="VFI12_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET7055425.1"
                     /translation="MRTGLARILLFLLLGLMAMPGLDAVSAQTTTKTTATTKTVNVEL
                     ILDSSGSMAERLDSGQTRIEAAKQVLTNVIGELPEQPNINVGFRVYGHKGNNTEAGKS
                     VSCRSTELKVPIKGVDKKALQDQVDAYQPVGWTPLSLSLREAGKDFPEADANTVNAVV
                     LVTDGLETCGGDPCSASRQIKTGASAITTHVIGFALDETERANLQCIVDESGGLLLGA
                     GDADELSAALFTVLEELQVVVKNGFIEIESIGGVFPKAKIDGQAEVTDSNPSGGEPFS
                     TTLTTENRVEVPAGSYDVHWQNPSGAETHITVTVEPDKTAFIRGSIFRFPHGGGEVYR
                     FTDLAGVIIWEDQIEFGDAVWVLPGVYRLQITELTGTPILLSMDVQTLPGEVTEVQVL
                     TAP"
     gene            1856..2962
                     /locus_tag="VFI12_03135"
     CDS             1856..2962
                     /locus_tag="VFI12_03135"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET7055426.1"
                     /translation="MADNLRIGIIGCGIGDFHAEAYELDRRADVVSLAGLDIPRCLEV
                     CKKHGIPEHTTHYEDLLARADVDAVSICVPNYLHAPIAIAALRAGKHVLLEKPIGLTA
                     AEGEQIVAEANAAGKVLGIAFSKRYRADVELLKAQTRAGALGEIYYAKAFWMRRAGIP
                     GLGSWFTNKGLSGGGSLIDLGVHVIDMVLWLMGNPTITSVSAATFSKLGPKGRGHWAG
                     HRFQHNAENAFEVDDLATAMLRTDTGAVIQLETSWATYSSHTDDHGVILMGDEGGAEI
                     RIHDYQKSGALKFFTDMNGVPVDTQPRTVVQHEHLQVVKRFIDAITLGVPMTPSGQEG
                     VDRARIIDSIYQSAALGHEITINQPVATTATAAD"
     gene            2975..>3430
                     /locus_tag="VFI12_03140"
     CDS             2975..>3430
                     /locus_tag="VFI12_03140"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HET7055427.1"
                     /translation="MTLRVTVWNEYRHERQNEAIGAIYPDGMHTAIANRLRKETDFDV
                     RTATLDEPAHGLTQDVLDSTDVLTWWGHKAHAEVADEVVERIKERVLAGMGLLVLHSA
                     HYSKIFKSLMGTSCSLKWREANDNERIWIVNPGHPITRGLPEHIDIEREE"
BASE COUNT          656 a         1111 c         1101 g          562 t
ORIGIN      
        1 ggacgcgcgt ccaggaagcg ctggtcacgg tggggatccc ggttctgcgc aaccagacaa
       61 cggttgtgcg ctacggcggc acgcagttgg cgatcgcagg gatcgacgag acgctgctcg
      121 gcgatcctga gccggccgcg acgatcgccc aggccccgcc cggcgtcccg gtcctcgcgc
      181 tctggcacga acccgaattt gccgagcaag cggcggcagc cggcgcattc gcccagctct
      241 ccggtcacac gcacggcggc cagatccgcc tccccgggat cggaccggtt gggctgcccg
      301 tccacggtca ccgccacatc attggtgagg ggcgggccga cggcatgccg atctacacct
      361 cgcgcggcgt cggcgtctac cgtccaccgg cccggttcaa ctgtccgccc gaggtcaccc
      421 tcgtcaccct cgtcgccccg actacctgac ccgttcccga ttccagcgta ggatgaccgg
      481 acgcgagcgc acgtattcgg ggcccgctca tttcggttgg ctggaggtga ctatgcggac
      541 tggtttggca cggatacttc tgttcctgtt gctcgggctg atggcgatgc ccgggttgga
      601 tgcggtcagc gcgcaaacga cgaccaagac gaccgcaacg accaaaacgg tgaacgtcga
      661 gctgatcctc gattcgtcgg gctcgatggc cgagcgattg gattccggac agacccgaat
      721 cgaggcggca aagcaggtcc tgaccaacgt cattggcgag ttgcccgagc aaccaaatat
      781 caacgtgggc ttccgggtct acgggcacaa gggcaacaac accgaggccg gcaagagcgt
      841 cagctgccgc tcgaccgagc tcaaggtgcc gatcaagggc gtcgacaaga aggcgctaca
      901 ggatcaggta gatgcctacc agccggtggg gtggactccg ctttccctct ctttgcgcga
      961 ggccggcaag gactttccgg aggctgacgc caataccgta aacgcggtcg tcctcgtgac
     1021 cgacgggctc gagacgtgcg gcggcgatcc ctgctcggcg tctcgccaga tcaagaccgg
     1081 cgcctcggcg atcaccaccc atgtcatcgg attcgcgctc gacgagaccg agcgggccaa
     1141 tctccagtgc atcgtggacg aaagcggcgg actgctcctc ggcgcgggag atgccgacga
     1201 gctgagcgct gcgctcttca ccgtgctcga agaactccag gtggttgtca agaacggctt
     1261 catcgaaatc gagtcgatcg gcggcgtgtt cccgaaggcg aagatcgacg gccaggccga
     1321 ggtgaccgac agcaacccgt ctggcggcga gccgttctca acgacgctga ccaccgagaa
     1381 ccgcgtcgaa gtgcccgcgg ggagctacga cgtccactgg cagaatccga gcggtgcgga
     1441 gacgcacatc acagtcacgg tcgagccgga caagacggca ttcatccgtg gctcaatctt
     1501 ccgcttcccg cacggcggcg gcgaggtcta ccgattcacg gaccttgccg gggtgatcat
     1561 ttgggaggat cagatcgagt tcggcgatgc cgtctgggtc ctgcccggtg tgtaccggct
     1621 gcaaattacc gagctgaccg ggacgccgat tctgctctcg atggacgtcc agacgcttcc
     1681 gggcgaagtg accgaagtgc aagtgctgac cgcgccgtga tccacgattt gcacacgttg
     1741 catcgctaac tctctcgtgt cagtctatga agtgcagtgc ggcgccgggc ctttccggcg
     1801 cccgccaccg ctgtcgcaac gcgcctgacc agcgccggga gaaaactgag tctgtatggc
     1861 tgacaacttg agaatcggca tcatcggctg cggtatcggc gactttcacg cggaggcgta
     1921 tgagctcgat cgccgcgccg atgtcgtttc cctggcgggg ctggacattc cgcgctgcct
     1981 cgaagtttgc aagaagcacg gcatccccga gcacacgacc cattacgagg acctcctggc
     2041 ccgcgcggat gttgacgcag tcagcatttg cgtaccgaat tatctgcacg ctcccatcgc
     2101 aatcgcggcc ctgcgcgccg gcaagcacgt cctgctcgag aagccgatcg gtctgaccgc
     2161 ggccgagggc gagcagatcg tggccgaggc aaatgcggcg ggcaaggtgc tcggcatcgc
     2221 gttcagcaag cggtatcgcg cggacgtcga actgctcaag gcgcagaccc gcgccggcgc
     2281 cctgggcgag atttactatg ccaaggcgtt ttggatgcgc agggccggga tcccgggcct
     2341 cggaagctgg ttcaccaaca agggcctctc cggcggcggc tcgctgatcg acctcggcgt
     2401 ccacgtgatc gacatggtcc tttggctgat gggcaacccg acgatcacga gcgtttcggc
     2461 cgccacgttc tccaagctcg ggccgaaggg ccgcggccac tgggccgggc accgcttcca
     2521 gcataacgcc gagaatgcgt tcgaggtcga tgacctcgcc acggccatgc tccggaccga
     2581 cactggcgcg gtgatccaac tcgagacgag ctgggcgacc tacagctccc ataccgacga
     2641 ccacggcgtg atcctgatgg gcgacgaggg cggcgccgag atccggattc acgactacca
     2701 gaaatccggc gccctgaagt tctttacgga catgaacggc gtgccagttg acacccagcc
     2761 gcgcacagtc gttcagcacg agcacctgca ggtagtcaag cggttcatcg acgcgatcac
     2821 gctcggcgtg ccaatgactc caagtggaca ggagggcgtg gaccgggccc gaatcatcga
     2881 ctcgatctac cagtcggccg cactcgggca cgaaatcacg atcaaccagc cggtggcgac
     2941 gaccgcgacc gcagcggatt aggagagcat cagcatgacg ctgcgcgtta cggtttggaa
     3001 cgaataccgc cacgagcgcc agaacgaggc gatcggcgcg atatacccgg acggcatgca
     3061 tacggcgatc gcgaatcgtc ttcgcaagga gacggatttc gacgtccgga ccgccacgct
     3121 cgacgaacca gcgcacggcc tgacccagga tgtcctcgat tcgaccgacg tcctgacctg
     3181 gtggggccac aaggcgcacg ccgaggtcgc cgacgaagtc gtcgagcgga tcaaggagcg
     3241 ggttttagcc gggatgggcc tgctcgtgct ccactccgct cattactcga agatcttcaa
     3301 gtcgctgatg ggcacgagtt gcagcctcaa gtggcgcgag gcaaacgaca acgagcggat
     3361 ctggatcgtc aatccaggtc acccgatcac caggggcctg cccgagcata tcgatatcga
     3421 gcgcgaggag
//
LOCUS       DASSGX010000176         2231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_685867, whole genome shotgun sequence.
ACCESSION   DASSGX010000176 DASSGX010000000
VERSION     DASSGX010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685867"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VFI12_03145"
     CDS             complement(<1..555)
                     /locus_tag="VFI12_03145"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET7055428.1"
                     /translation="MPVQLSSVYQDALALAIELHGNQTRKGSDIPYISHLLAVSATVL
                     EYGGTEEEAIAALLHDAVEDAGGAEIKKRIADQFGDNVARIVEACSDDSQPLGHTKRP
                     WRIRKNHHIEQLRKAPSSVLLITAADKLHNARSLMSDYRQLGDAVWDRFNAEPEDILW
                     YYRTVMEALDKGDVRAHKADGDDPP"
     gene            716..1615
                     /locus_tag="VFI12_03150"
     CDS             716..1615
                     /locus_tag="VFI12_03150"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HET7055429.1"
                     /translation="MAESQSGASESGLRLRPGMSLIAAAAAFRADLERKRRPRNTIES
                     YLFDLTVLAHQIPEKSINQITADDISRFLGEANTMSTRKRRLTSVRRFFRFLVDEATV
                     LSIDPTEDFYPNRIELRVPEPLSPADQQALLDAAAEDEPWSLTAILVMMDAGLSRGEL
                     LNLERGTIDRDSPYAVVIHVVTDDPRKPNQNRDLRASERLAKAYGAFLETRDPDGRLF
                     PYGFQAINGMVDRVRRSAGIGRAVTPRTLRDTFAVNRAINGASEDELIQELGLADDLR
                     NRQSIRRFLAFALQSPGSAPASN"
     gene            complement(1582..>2231)
                     /locus_tag="VFI12_03155"
     CDS             complement(1582..>2231)
                     /locus_tag="VFI12_03155"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HET7055430.1"
                     /translation="GPKDIGNTTRLAIQSLQSGRPWSEAGQFALDQMGSHSAASNGSV
                     MRCAPVAIRFRDDPGRLKKLSIDTARITHAEPRCTWAAVALNQAIAHALNGGSPDGLA
                     DAATAGIEQPDVVQAILTAPGKTAAGLDGSGYVLNAVQIAIWATLTQLSLEEAVVSAV
                     MIGKDTDTNAAVTGALAGAVHGYAAIPERWRTAIHQHDRLISLADQLLAGADPGD"
BASE COUNT          366 a          717 c          739 g          409 t
ORIGIN      
        1 ggggggatca tcgccatctg ccttgtgcgc tcgcacatca cccttgtcga gcgcttccat
       61 gaccgtccgg tagtaccaga ggatgtcctc cggctccgcg ttgaagcgat cccagaccgc
      121 gtcgccgagc tggcgatagt cggacatgag tgaccgtgcg ttatgaagct tgtccgcggc
      181 cgtaatgagt agtaccgagg acggcgcctt cctaagttgc tcaatgtggt ggttcttgcg
      241 aatgcgccac ggtcgcttcg tgtggccgag cggttggctg tcgtccgagc acgcctccac
      301 gatgcgcgcg acgttgtccc cgaactgatc cgcgattcgc ttcttgatct ccgcgccacc
      361 ggcgtcctcg accgcatcgt ggagcagggc ggcaatcgct tcttcctcgg tgccgccgta
      421 ttccaggaca gtggcggaga cggcgagcag gtgtgagata taggggatgt cggagccctt
      481 gcgggtctgg ttcccatgca gttcgattgc gagcgcgagg gcatcctggt agacggatga
      541 gagttgaacc ggcatggcag tgctcctcaa tccgagacgg accgtgagag tggcatttcc
      601 atgataacgg tggagacaac ggaggttgcg cttggcccgg ccagcagata gagtgagcga
      661 catatccctg tggcgaccga tcggccaatg agttcagccg ggaaaggaga gcgatatggc
      721 ggagtcgcag tccggtgcgt cggaatccgg tcttcggctg cggccaggga tgagcctgat
      781 cgccgccgcg gccgccttcc gcgccgacct cgagcgcaag cggcggccgc gcaacacgat
      841 cgagtcctac ctcttcgatc tgaccgtgct cgcgcaccag atcccggaga agtcgatcaa
      901 ccagatcacg gctgacgaca tatcccgttt cctgggcgag gcgaacacga tgtcgacccg
      961 gaagcggcgt ctgacgtcag tgcgtcgctt cttccgcttc ctggtcgacg aggcgacggt
     1021 cctctcgatc gacccgaccg aggacttcta cccgaaccgg atcgagctca gggtcccgga
     1081 accgctctcg ccggcagatc agcaggcatt gctggacgcg gcggccgaag atgagccatg
     1141 gagcctgact gcgatcctcg tgatgatgga cgccggtctt tcccgcggcg agctgctgaa
     1201 cctggagcgg ggcaccatcg accgggactc cccgtatgcg gtcgtcatcc acgtcgtcac
     1261 ggacgacccc cgcaagccga accagaaccg ggatctccgt gccagcgagc gactcgccaa
     1321 ggcgtacggc gcgttcctcg agacacggga tcccgatggt cggctcttcc catacgggtt
     1381 ccaggcgatc aacggcatgg tcgatcgcgt ccggcggtcg gctggcatcg gccgggcagt
     1441 gacgccacgc acgctgcgcg acaccttcgc ggtcaaccgg gcgatcaatg gcgcgagtga
     1501 ggacgagctg atccaggagc tgggcctggc cgatgacttg cgcaaccggc agagcatccg
     1561 ccggttcctc gcctttgcgc ttcagtcgcc ggggtccgcg cccgcgagca actgatcggc
     1621 gagcgagatg agtcggtcat gctggtggat cgccgtccgc caccgttcgg gaatcgccgc
     1681 gtatccgtgc accgccccgg caagcgcacc ggtcacggcg gcgttcgtgt cggtgtcctt
     1741 gccgatcatg accgcgctga ccacggcctc ctcgaggctc agctgagtca gggttgccca
     1801 gattgcgatc tggactgcat tcaggacgta gcccgaaccg tcgaggccgg cggccgtctt
     1861 ccccggcgct gtcaggatcg cctgaacgac gtctggttgc tcgattccgg cggttgccgc
     1921 gtcggccagc ccgtctggcg aaccaccgtt gagggcatgc gcgatcgcct ggttgagggc
     1981 gaccgccgcc cacgtgcagc gcggctcggc gtgggtgatc cgggcagtgt cgatcgacaa
     2041 cttcttgagc cggccgggat cgtcgcggaa gcgaatcgcg accggggcgc accgcatcac
     2101 tgatccgttg ctggccgccg agtgggagcc catctgatcg agcgcaaatt gtcccgcctc
     2161 gctccagggc ctgcccgatt gcagcgactg gattgccagt cgagtcgtat tgccgatgtc
     2221 cttcggtcct t
//
LOCUS       DASSGX010000177         2251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_795741, whole genome shotgun sequence.
ACCESSION   DASSGX010000177 DASSGX010000000
VERSION     DASSGX010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795741"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            561..1529
                     /locus_tag="VFI12_03160"
     CDS             561..1529
                     /locus_tag="VFI12_03160"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET7055431.1"
                     /translation="MTLSWPTPTLADLRAAQIRIAPIAVRTPIIKLDWDEGPGELYLK
                     LELLQPIRAFKIRGAYNAMAALDRSEIEQGVWTASAGNHAQAVAMSARALDVPCTVYV
                     PDTAPRTKINNAIGYGARVVELPVMEWVDIFRNRGYAQASGFFVHPYSDPNVIAGQGV
                     CGLEILEDMPDVEAIIAPWGGGGLTSGIASATAAMAPHVGVMASEIETGAPLGPSLAA
                     GHPVEVPYRPSFADGIGTPFVNVEMFELAHRLGITAIGTTLEQTADAARILISRAAVT
                     PEGAAATPLAAALNGGAGSGKTVLVISGGNIDAATLQTILAGGTPE"
     gene            1640..2149
                     /gene="dps"
                     /locus_tag="VFI12_03165"
                     /gene_synonym="pexB"
     CDS             1640..2149
                     /gene="dps"
                     /locus_tag="VFI12_03165"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: protein motif:HMM:NF006975.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HET7055432.1"
                     /translation="MATATKQMTGKMFKTSIDIPEDTRAKVVEYLNQNLADAFDFMSQ
                     SKQAHWNVKGQNFWQLHKLFDEIAGEAVEWVDMVAERVTALGGYATGTARMAAAASQL
                     PEFPTDITEGMDFVRAMVERLAAYSNSAREGIDKTEKWGDVDTSDLLTEISRCADKYL
                     YFLEAHLRA"
BASE COUNT          425 a          731 c          701 g          394 t
ORIGIN      
        1 ttatgagcgc agtcatgatc cgtcggcgag aaacaaggtc gcattgctat gcgcctctac
       61 aagcggccgc gcctcgggcg ggcagatgcc atctgcccct acattcagcc gcggcccaac
      121 tgctcgcctg ctcgcattgc gcctgctcgc ctaaaaagag aatcggcgca atcgaattgc
      181 ttcgaccgcg ccgatcgggt tggggaggtg gcggtaggag ggttcctacc cgaagcgctg
      241 gttgctcgct tcgttgatcc cactatcgag cgtcaagatg aactgacgat gaatcggtcg
      301 ttggtctctc gtcattggtt ttccgttttc cgtgcgcctg accgctcgtt accgattcaa
      361 ttttgcaacc actatcaacc ccgggctgag aacccgacgc ctctccgagg cggacagcgg
      421 aagggtcgga agttgcgacg ggcggatctg ccccgcagat ccagacaatc tccgcgccct
      481 gcttctcgac tcctcgacgt ctcgactcac tcaacctgcg acactacccg cagttcgacg
      541 cgcacgttca aggaggaatc gtgacccttt cctggcctac gccgacgttg gctgatctcc
      601 gggctgccca aatccgcatc gctcccatcg ccgtccgcac acccatcatc aagctcgact
      661 gggatgaggg tcccggcgag ctctatctga aactcgagtt gcttcaacca attcgcgcct
      721 tcaaaatccg gggcgcgtac aacgccatgg ccgcactcga ccggtccgag atcgagcagg
      781 gtgtctggac cgcgtcagcc ggcaaccatg cgcaagccgt cgccatgtcc gcccgggcgc
      841 tcgatgtccc ctgcaccgtg tacgtgcccg acaccgcgcc gcgcaccaag atcaacaacg
      901 cgatcggata cggcgcgcgg gtggtcgagc tgccggtcat ggagtgggtc gatatcttcc
      961 ggaatcgcgg atatgcgcag gcgagtggct tcttcgtcca tccctacagc gacccgaacg
     1021 taatcgcggg acaaggcgtc tgcggtcttg agatcctcga ggatatgccg gacgtcgaag
     1081 cgatcatcgc accgtggggc ggcggcgggc tcacgtcggg gatcgcctcc gcgaccgccg
     1141 cgatggcccc acatgtcggg gtcatggcca gcgagatcga aaccggcgcg ccacttggac
     1201 cgtcgctcgc agccggccac ccggtcgagg tgccctaccg gcccagcttc gccgacggca
     1261 tcggcacgcc cttcgtcaac gtcgagatgt tcgagctcgc ccatcgcctc ggaatcacgg
     1321 cgatcgggac caccctggag cagacggccg acgccgctcg gatcctgatc agccgggccg
     1381 cggtcacccc ggagggcgcc gccgcgacac cgctggcggc cgcactgaat ggcggcgccg
     1441 ggagcggcaa gacggtgctg gtcatctccg gcggcaacat cgatgccgcg acgctccaga
     1501 cgatcctcgc cggcggcaca ccggagtaag tcgttcgtag gctcgcgcat ctgcctctcc
     1561 tgaccgcttt gctacggttg aacccgtacg gcgcgtaccc gattcaggca caccgcagcg
     1621 atatcaggag aggcagacca tggcaacggc aacgaagcag atgaccggga aaatgttcaa
     1681 gacgagcatt gatattcccg aagacacgcg ggcgaaggtc gtcgaatatc tgaaccagaa
     1741 cctggccgat gcgttcgatt tcatgagcca gagcaagcag gctcactgga acgtcaaggg
     1801 ccaaaacttc tggcaactcc acaagctctt cgacgagatc gccggtgagg cggtcgagtg
     1861 ggtcgacatg gtcgcggagc gggttacggc gcttggcgga tacgcgaccg gaacggccag
     1921 gatggccgcc gccgccagcc aactcccgga gttcccgact gacatcaccg agggcatgga
     1981 cttcgtgcgg gcgatggtcg agcggctcgc ggcctactcg aactcggccc gggaaggcat
     2041 cgacaagacc gagaagtggg gcgatgtgga cacgtcggat ctgctcaccg agatcagccg
     2101 ctgcgccgac aagtaccttt acttcctgga ggcgcatctc cgggcatagt ggtcaagtgg
     2161 ttagtggctg gtgaatagtg atttgtgggg ggcggtcaat atggccgccc cttgccgttg
     2221 gcggcggatt cccggacgga aacacccggg t
//
LOCUS       DASSGX010000178         3003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_33681, whole genome shotgun sequence.
ACCESSION   DASSGX010000178 DASSGX010000000
VERSION     DASSGX010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33681"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VFI12_03170"
     CDS             <1..531
                     /locus_tag="VFI12_03170"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF014820.4"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HET7055433.1"
                     /translation="IRGDTTVESLAKLKPAFTGAGTVTAGNAPGVNDGAAALVITSEE
                     WASSNHKPILARILSHAEAAWDAPCLAKTPAMAGEIALERAGLKSSDIDLFEINEAFA
                     NVALNASRMLGADPERVNVNGGAVALGHPIGASGARIVLTLALELKRRGGGLGLAAIC
                     SGGGQGDAIIIEVPAA"
     gene            528..1079
                     /locus_tag="VFI12_03175"
     CDS             528..1079
                     /locus_tag="VFI12_03175"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HET7055434.1"
                     /translation="MSERFPGAHILEVNGVAPTIAEDVFIAPGAVVIGDVEIGPGSSV
                     WFNAVLRGDVAPIRVGAGSNIQDLSLLHVDRGNPCIVGNNVTIGHNAIVHGTVLEDDV
                     TIAMGAIVLSRSRIGRGAVIAAGAVVSEGMEVEAGALMAGVPAVMKRALNPERRDQLA
                     AIGGLYVENAARFRATMKLVEEG"
     gene            1083..1856
                     /locus_tag="VFI12_03180"
     CDS             1083..1856
                     /locus_tag="VFI12_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET7055435.1"
                     /translation="MAVDVTIDGAIATVTLNRADALNAFNTQQLRDAIAAFAALKDDR
                     LVRAVILTGAGEKAFAAGADIKEMVGLDRDGALEFGRLGHGLTRAVETLPQPVIAAVN
                     GFAMGGGCELALAADFRLASENAVFGQPEVGLGIPPGWGGTQRLVRAIGPGMASELIL
                     TGRRVKADEALRIGLVNRVLPLVDLIPKAHEMADVIAKNSPRAVRSAKRLMQLAFNGQ
                     VTSGLDTELVGFADSFGTADQIEGMTAFVEKRSPTYSDD"
     gene            1862..2749
                     /locus_tag="VFI12_03185"
     CDS             1862..2749
                     /locus_tag="VFI12_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055436.1"
                     /translation="MTDTPLIEVNGKPPAGAACAAILARAGVRLRVFGVDETFGGLAL
                     VSRSILEELGIAGAVTAYPIDRVTERVLSEDSATDTTAPIDDLVAVQTPEIAAALLAA
                     AETGAASPAGDAVLRVDATEFRLGDLELQDDDFLPISALEQVVQLEWTGGRPQETIWI
                     RMTGDPLGDVRAHAALVVTPDRSALGLAVPTEALVQASITLPDVLHRLLAQPRVAAEI
                     PADEPVTTSTRLLKTNGAVHLSLNGDLRVRVGAAAGLAGPLVLDRELRSGMNAGLAIA
                     AALSEGRLSLARLSRLARP"
BASE COUNT          524 a          931 c         1011 g          537 t
ORIGIN      
        1 atccgcggcg acaccaccgt cgagtccctg gccaagctga agccggcctt cacgggcgct
       61 ggaaccgtca cagctggcaa cgcaccggga gtgaacgacg gcgcagccgc gctcgtcatc
      121 acctcggagg aatgggcgtc cagcaaccat aagccgattt tggcgcgaat cctgagccat
      181 gcagaggcgg cgtgggacgc accgtgtctg gccaagacgc cggccatggc gggtgaaatt
      241 gccctcgaac gagcggggct gaagagctct gacatcgacc tgttcgagat caacgaggcc
      301 ttcgccaatg tggctctgaa cgcttcccgg atgctcggtg cagaccccga acgggtgaac
      361 gtgaacgggg gcgcggtggc gctcggccac ccgatcggcg cgagtggcgc ccggatcgtc
      421 ctgaccctcg ccctggaatt gaagcggcgc ggtggagggc tcggtctggc cgcgatctgc
      481 tccggcggcg gccaaggcga tgcgatcatc atcgaggttc cggccgcatg agtgaacggt
      541 tccccggcgc gcacatcctc gaagtcaatg gagtagcgcc gacgatcgcc gaggacgttt
      601 tcatcgcccc cggtgcggtc gtcatcggtg atgtcgaaat cggaccggga agcagcgtct
      661 ggttcaacgc cgtcctgcgc ggggacgttg cgccgatccg ggttggggca ggcagcaaca
      721 tccaggacct gtcgctgctc cacgtcgatc gtggcaatcc ctgcattgtc ggcaacaatg
      781 tcacgatcgg gcacaacgcg attgtccatg gcacggtcct cgaggacgac gtgacgatcg
      841 cgatgggggc catcgtactc tcccgctccc gcatcgggcg cggcgcggtt atcgcggccg
      901 gagccgtagt ctccgagggc atggaggtcg aggcaggtgc attgatggcc ggcgtacctg
      961 ccgtcatgaa gcgggcgctg aatcccgagc ggcgagatca actggccgct atcggcggac
     1021 tgtatgtcga gaacgcggct cgtttccgtg ccaccatgaa actggttgag gaagggtagg
     1081 cagtggcagt agacgtaacg atcgacgggg caatcgcgac cgtcacgcta aaccgggccg
     1141 atgcgctcaa cgcgttcaac acgcagcagc tacgcgacgc aatcgccgcg ttcgccgcgc
     1201 tcaaggacga ccgcttggtt cgcgccgtga tcctgaccgg ggccggggag aaggcgttcg
     1261 ctgccggcgc cgacatcaag gagatggtcg ggctggacag ggatggcgcg ctcgagttcg
     1321 ggcggctcgg ccatggcttg acccgagcgg tcgagaccct cccgcaaccg gtgatcgccg
     1381 cggtcaacgg gttcgcgatg ggcggcggtt gcgagctggc gctggccgcg gactttcgcc
     1441 tcgccagcga gaacgccgtg tttggccaac ccgaggttgg attgggcatc ccaccgggtt
     1501 ggggtggtac ccaacgactc gtccgggcaa ttgggcctgg catggcgtcg gagttgatac
     1561 ttaccgggag gcgggtcaag gcagacgaag cgttgaggat cggacttgtc aatcgcgtcc
     1621 tgccgctcgt cgacctgatt ccgaaggcgc atgagatggc cgatgtgatc gcgaagaaca
     1681 gcccgcgtgc ggtcagatct gccaagcgct tgatgcagct ggccttcaac ggtcaggtga
     1741 cgtcagggct cgacacagag ctcgtgggat ttgccgattc gttcggcact gctgaccaga
     1801 tcgagggcat gaccgcgttc gtcgaaaagc ggtcgccaac ctattccgac gattgatctg
     1861 gatgactgat acgccgctga tcgaagtcaa tggaaagccg ccggccgggg ctgcatgcgc
     1921 cgcaatcctc gcccgagcag gagtccgcct gcgcgtcttc ggggtcgacg agaccttcgg
     1981 cggtttggcg ttagtatcgc gctccattct cgaggagctt gggattgcgg gcgcggtcac
     2041 cgcgtacccg atcgatcggg ttaccgaacg ggtactatcc gaggacagcg caacagacac
     2101 gaccgcacca attgacgacc tggtggcggt tcagacaccg gagatcgcgg ctgcgttgtt
     2161 ggccgcagcc gagaccggcg ccgcatcgcc tgccggcgac gccgtgctcc gtgttgatgc
     2221 aaccgagttt cggctcggcg atctcgaact tcaggacgac gattttctgc cgatctcagc
     2281 gcttgagcaa gtcgtgcagc tcgagtggac cggtggccgt ccgcaagaga cgatctggat
     2341 tcggatgacc ggtgatccac tcggtgatgt cagagcgcac gccgcactcg ttgtgacgcc
     2401 ggatcggtct gccctcggtt tggcagtgcc tactgaggca ttggttcagg cgtcgatcac
     2461 ccttccagat gtcctgcacc ggctcctggc gcaaccgagg gtcgcggccg aaatcccggc
     2521 ggacgagccc gtgacgacct ctactcgact tctcaagacg aacggagcgg ttcatctgtc
     2581 cctgaatggt gacttacgcg ttcgagtcgg cgctgccgca ggtctggccg ggccgctagt
     2641 gctggaccgg gagcttcgtt ccggcatgaa cgccggcctg gcgatcgccg ccgcgttgtc
     2701 ggaggggcgg ctctcccttg cccgcttgag ccgcctggct cgcccatagc ttgccgaggt
     2761 agtgaacccg ccgagttgac cgggatcggt acgttcggta ctgatgacta tttctcccgc
     2821 gcgtcgcaca atgaattcga acatacgagc cattccccgc gggagcgcca attcgatgcc
     2881 ggacgacctt caacgcattg ccgacttgct ccgaccgatt gggccgggcg agcgatgctt
     2941 gcagccgagc gcgctcggcc gatacatcag tgccgaccaa tgccaacgcc tcttccgcct
     3001 gga
//
LOCUS       DASSGX010000179         2780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1123280, whole genome shotgun sequence.
ACCESSION   DASSGX010000179 DASSGX010000000
VERSION     DASSGX010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2780
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123280"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VFI12_03190"
     CDS             <1..364
                     /locus_tag="VFI12_03190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HET7055437.1"
                     /translation="TDGPDTDFTAKLVDVYPDGKAWNLCDGIVRGRFRNGRGPAELLT
                     PGEVYAFEIDCWVTSNLFKAGHRIRLEIASSNFPRFDRNLNTGHPIGDDAEPRIATNR
                     IHHDANHPSHILLPVIPS"
     gene            complement(666..1451)
                     /locus_tag="VFI12_03195"
     CDS             complement(666..1451)
                     /locus_tag="VFI12_03195"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET7055438.1"
                     /translation="MKSTLATYLRFEVRANLRATISWTIGLSIYVGAVVSLYPSVRSA
                     LNIGAIPPGLREAFNITDFTYLAGFLSSELFGVILPLVLPFYGIVVLSNVVAGNEERG
                     RLDVLLGNPIPRWQLVAGTWIVVAVYLLAMVMVFGSVIWAVASALDLALSANEAYRAT
                     FALWPPALAFSTLALVVSTLARQRAIAIGVPGAVVLLSYLILVIGRLADPLSFVRYIS
                     AYHYYGFAILEGVWWGGVAVLLAATAALLALAIITFNRRDIYA"
     gene            complement(1448..2365)
                     /locus_tag="VFI12_03200"
     CDS             complement(1448..2365)
                     /locus_tag="VFI12_03200"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7055439.1"
                     /translation="MPLTDTAAIATYNLTKNYGANRGIRDVNLRVERGEIFGFLGPNG
                     AGKSTTLRTILGFMRPTSGRAEVLGMDSQHQTVEIHRRLGNLPSEFSLWDRMTGAEML
                     RFFVDLRGGDIRLDYAHELAERLEADLARPMRRLSRGNKQKIGLIQALYHQPPVVVLD
                     EPTTGLDPLMQDVFLDILRETRDRGQTVFFSSHILPEVEQVADRVGVIREGELVAIED
                     PQALTSKGFRSVRIQFAEPLSAEELAQLAALPGVERFKATGPTVTFTAHGAMDPIIKL
                     ASRHRIELFDAERPPLEEIFLSFYGETAA"
     gene            2482..2703
                     /locus_tag="VFI12_03205"
     CDS             2482..2703
                     /locus_tag="VFI12_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055440.1"
                     /translation="MTSDRHHDSEEERNQQLKAEHQAESDGDGLLGNIKTAFRSFAQP
                     FVTGDTDLDNDDQADLEQQRDLNDEESRR"
BASE COUNT          538 a          953 c          874 g          415 t
ORIGIN      
        1 aaccgacggc ccggatactg atttcacggc caagctggtc gatgtctatc cggacggcaa
       61 agcctggaac ctctgcgacg gcatcgtgcg cggtcgcttc cggaatgggc ggggaccggc
      121 cgagctgctg acgccgggcg aggtctacgc gttcgagatc gactgctggg tgacgtcgaa
      181 cctgttcaag gcggggcacc ggatccggct cgagatcgcc tcaagcaact tcccccgctt
      241 cgaccgcaac ctcaacaccg gccacccgat cggcgacgac gccgagccgc gcatcgccac
      301 caaccgcatc caccacgacg ccaaccaccc ctcgcacatc ctgctcccgg tgatcccgag
      361 ttaggttttg ggtgttaggt tttgggtgtt aggttttggg ttttggcgaa gatgttcact
      421 tggaggcgga ccgtcgcaag gtgaacatct tcgacatctt cagcaccttc gccccacatc
      481 gggcgaacgc gcatcgtctc gcacccgcgg tagggacgtg cgggggcacg tcctccgctc
      541 ccggccaacc cgtcccacgc tgaaccacgc gttgatcccc tcgtctgcca aaggcaaccc
      601 gccggtgatc cacggtgggt tcgcccccgc ggtcccctat atgacgattc ggcaccccga
      661 ctggatcacg cgtagatgtc gcggcggttg aaggtgatga tggccagggc cagcagcgcg
      721 gccgttgcgg cgagcaggac cgccacgccg ccccaccaga ccccttcgag gatcgcgaag
      781 ccgtaatagt ggtaggccga gatgtaccgc acgaacgaga gcgggtccgc cagccgcccg
      841 atcaccagga tcagataact gagcagcacg accgcgcccg ggaccccgat cgcgatcgcc
      901 cgctgccgcg ccagggtcga gacgaccagc gccagcgtgc tgaacgcgag cgccggcggc
      961 cagagcgcga aggtcgcccg gtaggcctcg tttgccgaaa gcgccaggtc cagcgccgac
     1021 gcgaccgccc agatgaccga accgaagacc atcaccatcg ccagcaagta gaccgcgacc
     1081 acgatccacg tcccggcgac cagttgccag cgcgggatcg ggttgccgag caggacatcc
     1141 agccggcccc gctcctcgtt cccggcaacc acgttcgaga gaaccacaat cccatagaag
     1201 ggcagcacca gcggcaggat cacgccgaac agctcgctcg aaaggaaacc ggcgaggtag
     1261 gtgaaatcgg tgatgttgaa cgcctcgcgc agccccggcg ggatcgcgcc gatgttcagc
     1321 gccgaccgca ccgacgggta cagcgaaacc accgcgccga catagatgct cagcccgatc
     1381 gtccaggaga tcgtcgcgcg gagattcgcg cggacctcga agcgcaggta ggtcgcgagg
     1441 gtcgatttca cgcggccgtc tcgccgtaga agctgaggaa gatctcctcc agcggcggtc
     1501 gctcggcatc gaacagctcg atccggtgcc ggctggccag cttgatgatc ggatccatcg
     1561 cgccgtgggc cgtgaaggtc acggtcggac cggtcgcctt gaaccgctcg acgcccggca
     1621 gcgctgcgag ctgtgccagc tcctccgcgc tcagcggctc ggcaaactgg atgcgaacgg
     1681 agcgaaagcc cttgctggtc agcgcctgcg ggtcctcgat cgcgaccagc tcgccctccc
     1741 ggatcacccc gacccggtcc gcgacttgtt cgacctccgg caggatgtgc gacgagaaga
     1801 agacggtctg gccgcgatcg cgcgtctccc gcaagatgtc gaggaagacg tcctgcatga
     1861 gcggatcgag tccggtcgtc ggctcatcga gaacgaccac cggcggctgg tggtaaagcg
     1921 cctggatgag cccgatcttc tgcttgttgc cgcgcgagag gcggcgcatt ggccgggcca
     1981 ggtccgcctc gagccgctcg gccagctcat gcgcgtagtc gagccggata tccccaccgc
     2041 gcagatcgac gaagaagcgc agcatctccg cgccggtcat cctgtcccag aggctgaatt
     2101 cgctcgggag gttgccgaga cggcggtgga tctcgaccgt ctgatgctgc gaatccatgc
     2161 cgaggacttc ggcccgcccg ctggtcggcc gcatgaagcc gaggatcgtc cgcagcgtgg
     2221 tcgatttccc ggccccgttc ggcccaagga agccgaaaat ctcgccccgc tccacccgca
     2281 ggttgacatc gcggatgccc cggttcgcgc cgtagttctt ggtcaggttg taggtagcga
     2341 tggcggcggt atcagtcaag ggcaagccgg atctccgggc ggtgcggcgt aatcggaacg
     2401 ccgcaaatgc gcgtatcaat ccattatcgt tgacggtaat aaccgaaccc ggcacgaccg
     2461 cgacgcagca ggagatcagc catgaccagc gaccggcacc acgactccga agaagagcgc
     2521 aaccagcaac tcaaggccga gcaccaggcc gagtccgacg gcgacggtct gttgggcaac
     2581 atcaagaccg cgttccgctc cttcgcgcag ccgttcgtga ccggcgacac cgacctcgac
     2641 aacgacgacc aggccgacct cgaacaacaa cgcgacctca acgacgaaga atcccgccgc
     2701 tagggcggtg ttcggagcat tccgccaaaa acaatgggac atcccggcat cgcccgggcg
     2761 taaacacccg ggctgaatca
//
LOCUS       DASSGX010000180         4292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_686402, whole genome shotgun sequence.
ACCESSION   DASSGX010000180 DASSGX010000000
VERSION     DASSGX010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686402"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VFI12_03210"
     CDS             <1..573
                     /locus_tag="VFI12_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055441.1"
                     /translation="LPAYATIILIATSAGAMLDTVVSRCAEIALQLVPTGEIVAALEH
                     PEAQSVAALAGGRPGWAITALNDPDWRARQGEAIESLEAWLRMSKGDRLVEAYQRGDR
                     FMRERRQTLGDLDQLQLLWRDIVLSSAQLPQYAFNPARAERMIQRSTADCSDWHRALA
                     ATQQCIKDLSGNIRPRLAMQAMVNQWPTLS"
     gene            555..1658
                     /gene="ricT"
                     /locus_tag="VFI12_03215"
     CDS             555..1658
                     /gene="ricT"
                     /locus_tag="VFI12_03215"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HET7055442.1"
                     /translation="MADPILNPSANGSAADDRQAMIDNARQQARQRFKNRFDAGLPVA
                     GCECETETEQDGPSARVAGVRFLDSGQTYFFDPVAFELEYGDWVVVETNRGLEAGKVA
                     IAPSQMLQASLSGSLKPVIKLLGEEEIERMQALKREAAKAVKTFGAKIRERNLPMKPI
                     SAEYNYDGSHLTLNFTAPERVDFRDLARDLGSNFKCRVELRQVGPRDEARLLGGVGRC
                     GRTLCCASWLPVFPEVSMNMAKTQDLPLNPSKVSGVCGRLLCCLSYENDQYKQQKNLL
                     PKLGQPVQTPMGDGMVISLQVLRELVTVRYQDETPDQTFTAADLGLQPKSERTQRPDV
                     QRSAESGEGEPAQRRRRRRGRGRGQTQNPEAQV"
     gene            1660..1965
                     /locus_tag="VFI12_03220"
     CDS             1660..1965
                     /locus_tag="VFI12_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055443.1"
                     /translation="MPLYLVEHIPYQDGAEETIAPPTDLEGLARYSLDAMHGARWLTT
                     FTPDLHDDRHFSIWEAPDVEEIRAVMARFGFLSDGVVKAFSIRQWGPDDVISEHAPQ"
     gene            2043..>4292
                     /locus_tag="VFI12_03225"
     CDS             2043..>4292
                     /locus_tag="VFI12_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET7055444.1"
                     /translation="MIDSTVTSHRLLGRSSKRIDGERKVTGAEKFTADLRLPGMLFAR
                     PVTSPYPHARVRSMNKDAALALPGVVAVLTAEDLPIRRPFSSLPGKSPLAFGEIAFAG
                     QFVAIVLAENDQAAQDAVELVEVDYEELPAVPDFDSGLAEDAPAARVDTVDTNSDEAA
                     AHNADAATQTAEAEQPTSANVSSTLKFERGDVDAGFANAARTAEVTIRSESVHQGYIE
                     PQVAMASIDPVGEITIYTSTQGAFLGRARAAEWLGRPVGDIVVKTMPVGGGFGGKFIL
                     LEPLTAALAVATNRPVLLEFGRTDDLAASNPAPACEIAVKIGADASGKLTALKGDLTF
                     DTGSMSGSPLQIAAILLGGYYKFDNLLITGREVITNRAPAGAYRAPGAQQATYAIESA
                     IDDLAEALGIDPLEFRLRNCAEEGDLRPNGGAWPRIGLKETLEALRNHPAWQNRQPGT
                     GIAIGGWPGGIEPATSICRLDHDGKFTVVVGSADISGVNTGFRKIAAEVLGMEEEDVV
                     VVGADTAAAPYAGASGGSKVTYTVGAAVMKAAQDARQQVLQIAAQHLEANPEDLEIAD
                     GQVRVKGVPNASVGLKDIANMSMSFAGKYEPVYGRGATAITESAPGFAAHLANVSVDE
                     ETGEVQVNHYVAAQDVGFAINPALVEGQMMGAVAQGIGWALYEAIEYDETGQLVTGTL
                     TDYTLPRSNDIPPIETILVEVPSVHGPFGAKGVGEPPAIPGPATIRNAIKHASGARLS
                     TLPMRPERVL"
BASE COUNT          770 a         1385 c         1442 g          695 t
ORIGIN      
        1 ttgccggcgt acgccaccat catcctgatc gcgaccagtg ccggcgccat gctcgacacc
       61 gtcgtctcgc gctgcgcaga gattgccctg cagctggtcc cgaccggcga gatcgtggct
      121 gcccttgaac atccggaggc gcaatcggtc gcagcactgg cggggggcag gcccggctgg
      181 gcaattaccg cgctcaacga tcccgactgg cgcgcgaggc agggagaagc gatcgagagc
      241 ctcgaggcgt ggctgcgcat gtcgaagggt gaccggctgg ttgaggcata ccagcggggc
      301 gaccgtttca tgcgagagcg ccggcagacg ctgggcgatc tcgaccaact ccaactgctg
      361 tggcgggaca tcgtgctgag cagcgcccaa ctcccgcagt acgcgttcaa tcctgcccgg
      421 gctgaacgca tgatccagcg ttcgacggct gactgctccg actggcatcg ggcgctggcc
      481 gcgacgcaac aatgcatcaa ggacttgtcc ggaaatattc gaccacgact cgcaatgcaa
      541 gctatggtga atcaatggcc gaccctatcc tgaacccgtc cgcgaacggg tccgccgccg
      601 atgatcggca ggcaatgatc gacaacgcgc ggcagcaggc tcgccagcgc ttcaagaacc
      661 gcttcgacgc cggtcttccc gtcgcgggct gcgagtgcga aaccgagacg gagcaggacg
      721 gtccgtcggc ccgggtcgcg ggcgtgcggt ttctcgattc gggccagacc tatttcttcg
      781 atccagtcgc gttcgagctc gagtacggtg attgggttgt cgtcgagacg aaccgcgggc
      841 tggaggccgg caaagtcgcg atcgccccgt cgcaaatgct gcaggcgtcg ctttcgggca
      901 gcctcaagcc ggtgatcaaa ctcctcggtg aggaagagat agagcggatg caggcgctca
      961 agcgggaggc ggcaaaggcg gtcaagacat tcggtgccaa gatccgcgag cgcaatttgc
     1021 cgatgaagcc aatttcggcc gagtataact acgacggctc ccacctgacg ctgaacttca
     1081 ccgcgccgga acgggtcgat tttcgggacc tcgcccggga ccttggctca aacttcaagt
     1141 gccgggtcga gctccgccag gtcggcccgc gcgatgaggc ccggttgctc ggtggggtcg
     1201 gtcgctgcgg gcggacgttg tgctgcgcat cctggttgcc ggttttcccc gaagtttcga
     1261 tgaacatggc aaagacgcag gatctcccgc tcaacccgag caaggtctct ggcgtctgtg
     1321 gccgtctgct gtgctgcctg tcgtatgaga acgaccagta caagcagcag aaaaacctgc
     1381 tgccaaagct cggccaaccg gtgcagacgc cgatgggaga tggcatggtc atctcgctgc
     1441 aggtgctgcg cgagctggtg accgtgcgct accaggacga aacgccggac cagaccttta
     1501 ctgcggcgga cctcgggctc cagcccaaat ccgagcggac ccaacgacct gatgtccagc
     1561 gctcggccga aagcggggaa ggtgagcctg cccagcgacg tcgccggcga cggggtcgcg
     1621 gtcgcggcca gacgcagaat ccggaagcgc aggtctgaga tgccgctcta cctggtggag
     1681 cacatcccgt atcaggacgg cgcggaggag acgatcgcgc cgccgaccga cctcgagggg
     1741 ctcgcgcgct attcgctcga tgccatgcat ggcgcgcgct ggttgaccac ctttacgccg
     1801 gatctccatg acgatcgcca cttcagcatc tgggaagccc cggatgtcga ggagatccgg
     1861 gccgtcatgg cgcgattcgg attcctcagc gatggcgtcg tcaaggcgtt ttccatccgg
     1921 cagtggggcc ccgacgacgt gatttccgaa cacgcgccgc agtaaccctg ttggtagaat
     1981 ccccgtacgc tgttcgacgc gggggcgttc tgcgtgcccg cccacttttc cggagcaatt
     2041 gaatgattga ctcgactgtg accagtcacc gactgctggg tcgctcatcg aagcgcatcg
     2101 atggcgaacg taaggtcacc ggcgccgaaa agttcaccgc cgatctccgc ctgcccggca
     2161 tgctcttcgc ccggccggta accagcccgt acccgcacgc ccgggttcgc tcgatgaaca
     2221 aggacgccgc gttggcattg cccggcgtgg tggccgtcct gaccgccgag gatctgccga
     2281 ttcggcggcc cttctcgtcg ctgccgggca agtcgccgct ggccttcggc gagatcgcct
     2341 tcgccggaca gttcgtcgcg atcgtcctcg ccgagaatga tcaggcagcg caggacgcgg
     2401 ttgagctggt cgaggtcgac tacgaagagc ttccggccgt gcccgatttc gattccgggc
     2461 tggccgagga cgcaccggcg gcgagagtcg acaccgtcga cacgaacagc gacgaggcgg
     2521 cggcccacaa cgccgatgcc gccacgcaga ccgctgaggc agagcagcca acatcggcca
     2581 acgtctcgtc cacgctcaag ttcgagcgcg gcgatgtcga cgccggattc gcgaacgccg
     2641 ccaggactgc ggaggtgacg attcgctccg agtcggtcca ccaggggtac atcgagccgc
     2701 aggttgccat ggcgtcgatc gatccggtgg gtgagatcac gatctacacc agtacccagg
     2761 gtgcgttcct tgggcgcgcg cgggcggcgg agtggctcgg acggccggtc ggcgacatcg
     2821 tcgtcaagac catgcccgtc ggtggtggct tcggcggcaa gttcatcctg ctcgagccgt
     2881 tgacggccgc acttgcggtc gccacgaacc ggccggtgct gctcgagttc ggccgcaccg
     2941 atgacctggc cgcttccaat ccagcaccgg cctgcgagat cgcggtcaag atcggcgccg
     3001 acgccagcgg caagctgacc gcgctcaagg gcgatctaac cttcgacacc ggctccatgt
     3061 cggggtcgcc tctccagatc gccgcaatcc tgctcggcgg gtactacaag ttcgacaacc
     3121 tgctgatcac cggtcgcgag gtgattacga atcgcgcgcc tgccggagcc taccgggccc
     3181 ccggggcgca acaggcgacg tacgcaattg agtcggcgat cgatgacctg gcagaggcgc
     3241 tggggatcga tccgcttgag tttcgacttc gcaactgcgc cgaagagggc gacctccgcc
     3301 cgaacggcgg cgcgtggccc cggatcgggc tgaaggagac actcgaagcg ctccgcaatc
     3361 acccggcctg gcagaaccgg cagccgggta ccggcattgc gatcggcggg tggccgggtg
     3421 gcatcgagcc ggcgacctcg atctgccggc tggaccacga tggcaagttc acggtcgtcg
     3481 tcggatcggc cgatatcagc ggcgtgaaca ccggcttccg caagatcgcg gcggaagttc
     3541 tcggcatgga ggaagaggat gtcgtcgttg tcggcgcgga cacggcggct gccccgtacg
     3601 cgggcgcgag tggcggttcc aaggtcacct atacggtcgg cgccgcggtc atgaaggccg
     3661 cccaggatgc ccgccagcag gttctgcaga tcgcagccca gcacctggag gcgaaccccg
     3721 aagacctcga aattgctgat ggtcaagttc gcgtcaaggg cgtgccgaac gcgagcgtcg
     3781 ggctcaagga catcgccaat atgtcgatgt ccttcgccgg caagtacgag ccggtctacg
     3841 gtcgaggagc gacggcgatt accgagagcg cgcccggctt tgcggcacac ctcgcgaacg
     3901 tctcagtcga cgaggagacc ggcgaggtcc aggtcaatca ctacgtcgcg gcgcaggacg
     3961 tcggcttcgc gatcaacccg gccctggttg agggccagat gatgggcgcg gtcgcgcagg
     4021 gcatcggctg ggcgctctac gaggcgatcg agtacgacga gaccggccag ctggtgaccg
     4081 gaaccctgac cgactacacg ctgccacgga gtaacgacat cccaccgatc gagacgatcc
     4141 tggtggaggt gccgagcgtg catggaccgt tcggcgccaa gggtgtcggc gagccaccgg
     4201 cgatcccggg tcctgcgacg atccggaacg cgattaagca cgccagcggc gcgcggctgt
     4261 cgacgctgcc gatgcggccg gagcgggtcc tc
//
LOCUS       DASSGX010000181         2598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_905299, whole genome shotgun sequence.
ACCESSION   DASSGX010000181 DASSGX010000000
VERSION     DASSGX010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905299"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1715
                     /locus_tag="VFI12_03230"
     CDS             <1..1715
                     /locus_tag="VFI12_03230"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7055445.1"
                     /translation="LAALEAVVGHLDSGDGPPDPFELLDQITALVDQSLVRQLDRVAD
                     QSRFAMYQTIHEFAEGELAARDETDAARRAHAEWVVGLIETGAPMLAGPDQLDWLDRF
                     ETEHDNIRAALAFAIEERETDLAHRVAGLLWGFWSLRGHQQEGNRWYQRVLELPIDVP
                     TVDYAHCLRGAAEIASEQGRFAEAMEHYERALAIARSFGDSRQERQITGAMANAAYGQ
                     GSFDEATRLHREAIALHEAAGDRAALAKSFNNLGTVSLYLNRLDEAEAEYGRAREILV
                     ELGDRRSALVVLGNIGSVVRARGESERSVEIQREVLAGMREIGDERGIGSALSNLANA
                     YVELGDLDQAERYCRQSLEILEQLGLTRMIALAQLSLAQIAMGRGENVAAIGWAAESL
                     ANLAACGDIASSGVLLGMLGDLAFTIGEPVRSARLFGGAMAQIEAANAQDAIEDHAQY
                     ERTVGRTRDALGPERYAEEFAAGKATSLDELRRAARELQRIAADAPRDPKEAEIERRT
                     GLNGRDVQALRLFVAGNSNQEIADEIGMTLPEAVALVGRIYAKLGVESRAEVTAFAFK
                     NGLV"
     gene            complement(1795..2157)
                     /locus_tag="VFI12_03235"
     CDS             complement(1795..2157)
                     /locus_tag="VFI12_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055446.1"
                     /translation="MPSPHPQNPRPAGPTGFAISRRAAVWLTGIALFVAAMTWLSLYQ
                     IKVTAADHPPTQGEIAQCKAELDPNSTVDPDVACPPTAHVLWAARHPLRNAIVTFVVL
                     MILGYDVIILNRFLQPRG"
BASE COUNT          466 a          799 c          927 g          406 t
ORIGIN      
        1 atctggccgc gctcgaggcg gtggtcggtc acctcgattc cggtgacggc ccgccggacc
       61 cattcgagct ccttgaccag atcaccgcgc ttgtcgacca gagcctcgtg cggcagcttg
      121 atcgtgttgc tgaccaatca cgcttcgcga tgtaccagac gatccacgag ttcgcggagg
      181 gagagctggc ggcacgtgac gagaccgatg cggcccggcg cgcgcatgcc gagtgggtcg
      241 tcggtctgat cgagaccggc gcgccgatgc tggccggccc tgatcagctc gattggctgg
      301 accggttcga gacggagcat gacaacatcc gcgccgccct cgccttcgcg atcgaggagc
      361 gcgagaccga cctcgcacac cgggtcgccg gcctgctctg gggcttctgg agtttgcgtg
      421 gacatcagca ggagggcaat cgctggtatc agcgcgtcct cgaactaccg atcgacgtgc
      481 cgacggtcga ctacgcccat tgcctgcgtg gcgcggccga gatcgcgtcc gagcaggggc
      541 gtttcgctga agctatggag cattacgaac gggcgctcgc gatcgcccgc tcgttcgggg
      601 atagtcgcca ggagcggcag atcaccgggg cgatggcgaa tgccgcctac gggcagggca
      661 gtttcgacga agccacgcga ttgcaccgcg aggcgattgc cctgcatgag gcggctggcg
      721 atcgggcggc gctggccaag agcttcaaca acctgggcac ggtcagcttg tacctgaacc
      781 ggctggacga ggccgaagcg gagtacggcc gggcgcgcga gatcctcgta gagcttgggg
      841 atcgccggtc ggcgctggtc gtgctcggca acatcggcag tgtcgtccgc gcccggggcg
      901 aatccgagcg atccgtcgag atccagcgcg aggtcctggc cgggatgcgc gagatcggcg
      961 acgagcgcgg aatcggcagc gcgctgtcca atctggcgaa cgcctatgtc gagctcggcg
     1021 atctcgatca ggcagagcgc tactgccggc agtcactcga aatcctggag cagctcggcc
     1081 tgacacggat gatcgccctg gcgcaattgt ccctggccca gattgccatg gggcgcgggg
     1141 agaacgtcgc cgcgattggg tgggccgcgg aatcgctggc caacctcgcc gcttgcgggg
     1201 atatcgcgtc gagcggcgtg ctgctgggca tgctcgggga tctcgccttt accatcggcg
     1261 agccggtccg cagcgctcgg ttgttcggcg gggcaatggc tcagatcgag gcggccaacg
     1321 cgcaggacgc gatcgaggat cacgcgcagt atgagcgcac agtcggccgg acgcgggatg
     1381 cgctcggtcc tgagcgatat gctgaggagt tcgcggccgg caaggcaaca tccctggacg
     1441 agctccggcg ggccgcccgg gagttacagc gcatcgcggc ggatgcgccc cgcgatccga
     1501 aggaagcgga gatcgagcgg cggaccggat tgaacggccg cgacgtgcaa gcgttacgcc
     1561 tgttcgtcgc cggaaactcc aaccaggaga ttgccgacga gatcggcatg accctgccgg
     1621 aggcggtcgc cctcgtcggc cgcatctacg ccaagctcgg cgtcgaatcc cgtgccgaag
     1681 taacggcctt cgccttcaag aatggccttg tgtgagtcgc ggaggtgcgg agtcggggaa
     1741 cggaaaaccg aaaacggaaa accaaacacg agagaccaaa gaccaaagac ctcgtcagcc
     1801 gcggggttgc aggaagcggt tcaggatgat cacgtcgtag ccgaggatca tgagcacgac
     1861 gaaggtgacg atcgcgttgc ggagcgggtg gcgagcggcc cacaggacat gcgccgtcgg
     1921 gggacaggcg acgtcgggat cgacggtgct gttcggatcc agctcggctt tgcactgggc
     1981 gatctcgccc tgagtcggcg ggtggtccgc ggcggtgacc ttgatttgat agagggacaa
     2041 ccaggtcatt gccgcgacga agagcgcgat tccggtcagc cagaccgcag cgcggcggct
     2101 gattgcgaag ccggtcggtc cggccggtcg ggggttctga ggatggggtg acggcacagg
     2161 cgaaatccgg gtaacgaatc cgagctggcc atcccgatga cagccaatca atcctacccg
     2221 aacaacagcg gttgagggtt gagggtggag ggaaggaaaa gagcagcgtt gcatgacacc
     2281 gcgactgccc gttcccctgg cctgaagacc ggggctagaa gctgaacccg ggcaagacgc
     2341 tcgggaaacc atggtgcggg tgatgtctca cacgcggcgc gggtgcttgc atatcccctc
     2401 cggctctcct tgatacggag agcgcatccc gcgatcgcca gaacggccat gatcgtagcg
     2461 cggtgcggca gccccgcgtc cggaccccgt cccgatcggg tcgttcccgg gaaccaagcc
     2521 ccggtgtccg tatcgccggc atccactgcg cggggcgatc acggtagcgc ggggcttccg
     2581 caccgcgcta cgtcacgt
//
LOCUS       DASSGX010000182         2490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_251677, whole genome shotgun sequence.
ACCESSION   DASSGX010000182 DASSGX010000000
VERSION     DASSGX010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251677"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VFI12_03240"
     CDS             complement(<1..294)
                     /locus_tag="VFI12_03240"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HET7055447.1"
                     /translation="MEIRGEHRFSAPRERVWDLLLDPAILQQCLPGAEDLVEVGPEEY
                     EARMKIGVAAIRGTYQGRVKIQDKDEPNSYRMAVEGKGPAGQISGDARIELVVD"
     gene            495..1880
                     /locus_tag="VFI12_03245"
     CDS             495..1880
                     /locus_tag="VFI12_03245"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HET7055448.1"
                     /translation="MVTASPVRLDRGRIAELAAKEQKKLDERTEKSGEMYQWARRTLA
                     GGVASSYQVRDPWPIYLERGQGSKVWDVDGNEMVDFHNGFGSMVQGHANPAIVEAVQR
                     QVTRGTHFAAPVEDAIVVSEELAKRFGLPQWRYLNSGSEATMDAIRIARAVTGRDTIV
                     KIFGSYHGHHDYVMVSISVPYENIGDRDNLASLPSGAGIPKAASDLTIPVPFNDAPAM
                     ERRIERLFAEGRPPACVIMEPAMMNLGVVLPEPGYLEAVREITRRHGVVLIFDEVKTG
                     LCVAAGGATEKFGVKPDMVTMAKALGGGLPSGAIGGTEEVMEVVNNGEVYQVGTYNGN
                     PLTMAAARASLEKVLTPEAYLHLDMLNDHLMAGCDAVLEKYHLPGYTVGISSKGCVTF
                     SPTKIVDYASFQASHDGELTDLAWLYMMNRGVFMTPGREEEWTLSVAHSVEDADTYIA
                     AFEELAHDLVV"
     gene            1955..>2490
                     /locus_tag="VFI12_03250"
     CDS             1955..>2490
                     /locus_tag="VFI12_03250"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET7055449.1"
                     /translation="MIDLTHRLTPDIPRFPGDPDVRIEPLPGFAPWQVSALTMGTHSG
                     THMDAPRHRFPDAASIDRYGPDRLVGRGYVIDAPDLADNAAIPAAVLSSLGTHPAPGW
                     LAVLRTGWDRFWGDERYFRHPFLAPELAQTLVALGAGLVAIDTLSVDSTVDRGSVAHE
                     ILLGADVLIAENLVSLGS"
BASE COUNT          428 a          870 c          767 g          425 t
ORIGIN      
        1 gtccacaaca agctcgatcc tggcgtcgcc ggaaatctgc cccgccggtc ccttcccctc
       61 caccgccatg cggtacgagt tcggctcgtc cttgtcctga atcttgacac gtccctggta
      121 ggtcccccgg atcgcggcga cgccgatttt catccgcgcc tcgtactctt ccggaccgac
      181 ctcgacgaga tcctccgcgc cgggcagaca ctgctgcagg atcgccggat cgagcaggag
      241 atcccagaca cgctctcgcg gcgccgaaaa gcggtgttca ccgcggattt ccatctcgcg
      301 cgacgctcct cgttggcagc gtccggccgc gggccggtca acgcctggca aagtgaaacc
      361 ggccgcgagt gtagcatgga ctggaacgca cctggtttcg acagaaccgg gcgctccgga
      421 cgataatcgg ggcgttccaa agcttgtggc tcggcgctgc tgcccggcca ctacacagag
      481 agggataccc aggcatggtc acggcaagtc cagtgcgtct cgaccgcggt cgcatcgccg
      541 agctggcggc aaaagagcag aaaaagctcg acgagcggac tgagaaatcc ggtgagatgt
      601 atcagtgggc acggcgcacc ctcgccggcg gcgtcgcctc ctcatatcag gtccgcgacc
      661 cctggccgat ctatctggag cgagggcagg gctcgaaggt ctgggatgtc gacggcaatg
      721 aaatggtcga tttccacaac gggttcggct cgatggtcca gggccacgcc aaccccgcga
      781 tcgtcgaggc cgtgcaacgc caggtcaccc gcggcaccca cttcgcggcg ccggtcgaag
      841 acgcgattgt ggtcagcgag gagctagcga agcgcttcgg gctgccccaa tggcgttatc
      901 tcaactccgg ttccgaggcg acgatggacg ccatccgtat cgctcgcgcc gtcaccggcc
      961 gcgatacgat cgtcaagatc ttcggctcct accacggcca ccacgactac gtgatggtct
     1021 ccatctcggt cccgtacgag aacattggcg accgcgacaa tctcgcctcc ctcccctccg
     1081 gcgctggcat tcccaaagcc gcctcggatc tgaccatccc ggtgccgttc aacgacgcgc
     1141 cggccatgga gcgccgcatc gagcggctct ttgccgaggg gcggccgccg gcctgcgtca
     1201 tcatggaacc ggcgatgatg aatctcggcg tcgttctccc cgagccgggg tacctggaag
     1261 cggtgcgaga gatcacgcgc cggcacggcg tcgttctcat tttcgacgag gtcaagaccg
     1321 ggctgtgcgt ggccgccggc ggagccaccg aaaagttcgg cgtcaaacca gacatggtga
     1381 cgatggccaa ggcgctcggc ggtgggctcc cctccggcgc catcggcggc accgaagagg
     1441 tcatggaagt cgtcaacaac ggcgaggtct accaggtcgg cacctacaac gggaacccgc
     1501 tgacgatggc cgctgcgcgg gccagcctgg agaaggtcct cacccccgag gcctacctgc
     1561 atctcgacat gctgaacgac catttaatgg ccggctgcga cgccgtgctg gagaagtacc
     1621 acctccccgg ctacacggtc ggcatcagtt ccaaaggctg cgtgaccttt tcgccgacga
     1681 agatcgtcga ctacgcctcg ttccaggcga gccatgacgg cgagctgacc gatctggcct
     1741 ggctctacat gatgaaccgc ggcgtgttca tgacgccggg ccgcgaagag gagtggaccc
     1801 tctccgtcgc ccactccgtc gaggacgcgg acacctacat cgccgccttc gaggagctgg
     1861 cgcacgacct cgtggtctaa tcgcgttgct caacgctcag tcggggccgt gccgccaagc
     1921 ggcatggccc cggtgtttgc gccgagattg gctgatgatc gatctgaccc accgcttgac
     1981 gcctgatatt ccgcgcttcc cgggcgaccc cgacgtgcgc atcgagccgt tgcccggctt
     2041 cgcgccatgg caggtgagcg ccctgacgat gggcacccac agcggcaccc acatggacgc
     2101 gccccgacac cgatttcccg acgccgcgag catcgatcgg tacggccccg atcgtctcgt
     2161 cggccgcggc tacgtaatcg acgcgccgga tttggccgac aatgccgcga tccccgccgc
     2221 cgtgctgtcg agcctcggca ctcaccccgc tcccggctgg ctcgcggtcc tccgcaccgg
     2281 ctgggatcga ttctggggag acgagcgata cttccgacat cccttcctcg cacccgaatt
     2341 ggcgcagacg ctcgtggccc tcggcgccgg tcttgtcgcc atcgacaccc tcagcgtcga
     2401 ttcgacggtc gatcgcggct ccgtggcgca cgagatcctt ctcggcgccg atgtcctgat
     2461 cgctgaaaat ctcgtcagcc tgggatcgtt
//
LOCUS       DASSGX010000183         4229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_469261, whole genome shotgun sequence.
ACCESSION   DASSGX010000183 DASSGX010000000
VERSION     DASSGX010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469261"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>4229
                     /locus_tag="VFI12_03255"
     CDS             <1..>4229
                     /locus_tag="VFI12_03255"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET7055450.1"
                     /translation="ANINLPRGDYLLRVSGDPDPAAEYILRIDVTTPPSASFESEPND
                     SEELAATLDPNTPMSGRFDGDEWDVYRFTTSGDPQLWEVAVAGTGIGQLDFTKRDGTE
                     LSTGEINADRTSARLSDLYLTPGDHWLRVSGENGDYTITLTALGPPVPGGEHESNNLA
                     VTAEPMLVGETKTGRLVQNTDVDVFRFSLAANEHIVLALDAPDDGTVDLRLDWGNAHV
                     ASMASAKPGDRTRLDLMLLPGDYVVTLSPAQVSTGRYSFQVSREDPFAVSDDQEPNDT
                     IATAQRLPAGKEMRGELTGTDGHDIYLIAPGNGRKHTFALKGDGVYPASLSDDAGNSY
                     SLTPSVDGATYTVDQRIPDDVPLYLDLTGAGTYRLTYGASSGGGGLQLPSILGGGDKA
                     PLDASLVFETTEVAAYLTEAQAVDGTLTLTNTTQKQVDFVLLPATSHYHFSVDIDDAP
                     NSIGAGQTVDVPIHVAIEPDAWADIPVRITIGIEVGDVMTSAFTEITPTAAAAPANPT
                     QAWPIPFDVLGGFDLASPALGATVAGTVDPAGESLLHDGVAPVGSGLVVNSPVLPLTL
                     TVDLAGDAAAPVRGIILNPLAGDGMPQSRVRQLELQLSTDGVTYTTVWTGELSAIGRE
                     QAFLLPEPIEATHAQLVILSSYDTNPTAPSYLGLGEWSVIAEPGFVPETVDRINVADP
                     AFGGHIVRTDPQLQTPDEWQAMLDGDRTALREYPSETGAFSWVIGFQDDRAALVTDLA
                     WIDPDGTNPDERISKVTVEVTTGTPSGPWESLGTWTLKRNDKGGVRRFKLDAPAWARF
                     IRISGAVDPNASSVEYPAQIQIFEAQAGDGYRSVIGAWGGTSAAAMYELKNPPPTQLV
                     QDETDAPDQQDQAAPLALGEMVNGQAQGSVDVDWYRVTAPKDDNTLTFTLTGVPTVDV
                     TVRVSDSAGAEVPVTRAIGQDTSTIVYSATVTPGAEYTVKVAQPPHSIIFAFDTSMSM
                     GPYEPMVQASLRSFAAGIEKGQEFVQVMPFEEEPLLESWADDSYLVYSAINGYYDKSL
                     SSSSEATILDATNLLADQEGSTAILLVTDAETSSFDKGAEMWQHLDVVQPRIFAVHVA
                     GSSTPVLNEHLMQDWSSNGGYYQYTVNQAEMDRAFDRAATWLRRPAGYSLVVSSTSEE
                     PATPEPAATETPAPEPTPAQPGTLSVVAPPPGETGSTGAAQVSDQVTIEIILDTSGSM
                     LAAMPDGQRRIDVARNVLTDLVTTELPAGVPITLRTFGNQPDSCDTNQVIPVQPLDPA
                     TVAATIQGIEPVNLVRTPIGASLEQVANDLAGVDGPKIVVLVTDGEETCGGDPAAAIQ
                     SLIDQGIDVQVNIVGFALDDDALRAQFRDWARIGNGSYFDATNAEELDQAIAKAVLAP
                     FRVLDADGNVVASGTVGGDPVELPAGTYTVV"
BASE COUNT          736 a         1459 c         1389 g          645 t
ORIGIN      
        1 tcgccaacat caacctcccg cgcggcgact acttgcttcg cgtctccggc gatccggatc
       61 cggcggccga gtacatcctg cggatcgacg tcacgacgcc gccatccgcc agtttcgaga
      121 gtgagccgaa cgacagcgaa gaactggccg ccaccctgga cccgaacacg ccgatgtccg
      181 gccgcttcga cggcgacgag tgggacgttt accgcttcac gacgtcgggt gatccccagc
      241 tctgggaggt cgcggtcgcc ggcacgggga tcggccagct cgatttcacc aaacgcgacg
      301 gcaccgagct ctcgaccggc gaaatcaatg ccgatcgcac ctcggcgcgg ctctccgacc
      361 tctacctgac gcccggcgat cactggctcc gggtcagtgg cgagaacggc gactacacga
      421 tcacgctgac ggcgcttggt ccgcccgtgc ccggcggcga gcacgaatcc aacaatctgg
      481 cggtaaccgc cgagccgatg ctggtcggcg agaccaaaac cggccgcctg gtccagaaca
      541 cggatgtcga tgtctttcgc ttctcgctgg cggcgaacga gcacatcgtc ctcgcgctcg
      601 atgcgccgga cgatggcacg gtcgatctcc gcctcgattg gggcaatgcc cacgtcgcct
      661 cgatggcgtc ggccaagccc ggagaccgaa ccaggctcga cctgatgctg ctgccgggtg
      721 attacgtggt cacgctttct ccggcgcagg tcagcaccgg ccggtacagc ttccaggtct
      781 cgcgggagga cccattcgcg gtcagtgacg accaggagcc gaacgacacc atcgctaccg
      841 cccagcggtt gccggccggg aaggagatgc gcggcgagct gaccgggacc gacgggcacg
      901 atatctacct gatcgcgccg gggaacggcc gcaaacacac ctttgcgctg aagggggatg
      961 gggtctatcc ggcatcgctc tcggacgacg ccgggaactc ctactcactg acgccatcgg
     1021 tcgacggcgc cacctacact gttgatcagc gcatcccgga tgacgtcccg ctctacctcg
     1081 acctgaccgg cgcaggcacc tatcggctca cctacggcgc atcctccgga ggtggcggcc
     1141 tgcaactgcc gtcaatcctg ggcggagggg acaaggcgcc gctcgacgcc tcgctggtgt
     1201 tcgagacgac cgaagtcgcg gcctacctga ccgaggcgca ggccgtcgac gggactctga
     1261 ccctcacgaa tacgacccag aaacaggtcg atttcgtgtt gttgcccgca acatcgcact
     1321 atcacttcag cgtggatatc gatgacgcac cgaattcaat cggagcgggc caaaccgtgg
     1381 acgtgccgat ccatgtcgcg atcgagccgg acgcatgggc cgatatcccg gtgcggatca
     1441 cgatcggtat cgaggtcggc gacgtcatga ccagcgcgtt taccgagatc acgccgactg
     1501 ccgccgcagc gcccgccaac ccgacgcagg cgtggccgat tccctttgac gtgctcggcg
     1561 ggttcgacct cgcctcgccg gcgctcggcg caaccgtcgc gggcacggtc gatccggccg
     1621 gcgaatcgtt gctgcatgac ggggttgcgc cggtcggatc gggactcgtg gtcaacagcc
     1681 cggtcttgcc gttgactctg acggtcgatc tggccgggga tgcggcggcg ccggtgcgcg
     1741 ggatcatcct caacccgctc gcgggcgacg gcatgccgca atcacgcgtg cgccagctcg
     1801 agctgcagct ctcgaccgac ggcgtcacct acaccaccgt ctggacaggt gagctgtccg
     1861 ctatcggccg ggagcaggcg ttcctgctgc cggagccgat cgaggcgacc catgcccagc
     1921 tcgtgatcct gagcagttac gacaccaacc cgaccgcgcc gagctacctt gggctcggcg
     1981 aatggagcgt gatcgccgag cccggattcg tgccggagac tgttgaccgc atcaacgtgg
     2041 ccgatcccgc gttcggtggc cacatcgttc gcactgatcc tcaactccag acgccggatg
     2101 aatggcaggc gatgctggac ggggaccgga ccgcgctccg cgaatacccg tccgagaccg
     2161 gcgcattcag ctgggtgatc ggcttccagg acgaccgggc tgccctggtc acagacctcg
     2221 cctggatcga cccggacggc acgaacccgg acgagcgcat atcgaaggtg actgtcgagg
     2281 tcacgaccgg gaccccgtcc ggcccgtggg agtcgctcgg gacctggacc ctgaagcgga
     2341 atgacaaggg cggagttcgc cggttcaagc tcgacgctcc ggcgtgggcc cggtttatcc
     2401 ggatctcggg cgcggtcgac ccgaacgcgt cgtcagttga gtatccggcc cagatccaga
     2461 tcttcgaggc acaggccggg gacggctatc ggtcagtgat cggcgcgtgg ggcggcacca
     2521 gcgcggcggc gatgtacgag ctcaagaatc cgccgccgac ccagctcgtg caggacgaga
     2581 ccgatgcgcc cgaccagcag gatcaggccg cgccgctcgc gctcggcgag atggttaacg
     2641 gccaagccca ggggagcgtg gacgtcgact ggtaccgggt caccgcgccg aaggacgaca
     2701 acacgctcac gttcaccctg accggcgtgc cgaccgtcga cgtcacggtc cgtgtctccg
     2761 actccgcggg tgcggaggtc ccggtcacgc gcgcaatcgg tcaggacacc tcgaccatcg
     2821 tctattcggc gacggtcacg cccggcgcgg agtacacggt caaggttgcg cagccgccgc
     2881 actcgatcat cttcgcgttc gacacatcga tgagcatggg gccgtacgag ccgatggtgc
     2941 aggcgtcgct gcggagtttt gccgccggca tcgagaaggg gcaggagttc gtccaggtca
     3001 tgccgttcga ggaggagccg ctgcttgagt cgtgggccga tgactcctac ctcgtctact
     3061 cggcaatcaa cggctactac gacaagagcc tgtccagctc gtccgaggcg acgattctcg
     3121 acgcgacgaa tctgctcgcg gatcaggagg gatcgaccgc gatcttgctt gtgaccgacg
     3181 ccgagaccag cagcttcgac aagggcgccg agatgtggca acacctcgac gtcgtccagc
     3241 cgcgcatctt cgctgtccac gtcgcgggat cgagcacgcc cgtcctcaac gagcacctga
     3301 tgcaggactg gtcgagcaac gggggctatt accagtacac ggtgaaccag gcggagatgg
     3361 accgtgcctt cgaccgggcg gcgacctggc tgcgccggcc tgccggctac agcctcgttg
     3421 tctcgtccac ctccgaggag ccggccacgc ccgaaccggc cgcaaccgag acgccggcgc
     3481 cggagccaac cccggcccaa ccgggcaccc tcagcgtcgt cgccccacca ccgggcgaga
     3541 cgggcagcac cggcgcggcg caggtgtcgg accaggtcac gatcgagatc atcctcgaca
     3601 cctccggctc gatgctggcc gcgatgccag acgggcagcg ccggatcgac gtcgcgcgca
     3661 acgtgctgac ggacctcgtc acgaccgagc ttccggccgg cgtgccgatc acgctccgca
     3721 ccttcgggaa ccagccggac tcgtgcgaca cgaatcaggt gatccctgtc cagccgctcg
     3781 acccggccac tgtcgcagcg acaatccagg ggatcgagcc ggtcaacctc gttcggacgc
     3841 cgatcggcgc ctcactcgag caggtcgcga acgacctcgc cggcgtcgat gggccgaaga
     3901 tcgtcgtgct ggtcacggac ggagaggaga cctgcggcgg cgacccggcg gcggccatcc
     3961 agtcgctgat tgaccagggg atcgacgtcc aggtcaacat cgtcggtttc gcgctcgacg
     4021 acgatgccct gcgcgcgcag ttccgggact gggcgcggat cggcaacggc tcctacttcg
     4081 atgccaccaa cgccgaggag ctggaccagg cgatcgcgaa ggccgtcctg gccccgttcc
     4141 gggtgctgga cgcggacggc aatgtggtgg cgagcggcac ggtcggcggg gaccccgtgg
     4201 agctcccagc cggcacctac acggtcgtg
//
LOCUS       DASSGX010000184         2059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_469621, whole genome shotgun sequence.
ACCESSION   DASSGX010000184 DASSGX010000000
VERSION     DASSGX010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469621"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            440..1324
                     /locus_tag="VFI12_03260"
     CDS             440..1324
                     /locus_tag="VFI12_03260"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET7055451.1"
                     /translation="MLLFMAVAEERSFTRAAAKLGTSQSTLSHTIKQLEARLGLRLLT
                     RTTRSVSPTEAGERLLHSLAPRIHEIESDVDALMAFRDRPSGTVRITLSDHALDWIVW
                     PKLKPVLRDYPDIHVELNVDNGFRNIVEGRFDAGVRLGESVDKDMIAIRISPDWRLVA
                     VASPEYLALHKTPEAPQDLVGHNCINHRQPQAGGLYAWEFEKDGRELRVRVDGQLTFN
                     TSIAMVDAATGGLGIAYVPESLVMDPIRSGRLVRLLDEWCPKFPGYHLYYPTRRQNSP
                     AFAVIVNALRHTARTDTR"
     gene            1428..>2059
                     /locus_tag="VFI12_03265"
     CDS             1428..>2059
                     /locus_tag="VFI12_03265"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET7055452.1"
                     /translation="MLTLRQIEVIRAIMVTGSIAGAARLLNVAAPGISRLMKYSEDSL
                     GVRLFDRRAGRYVPTPQARHIFDLLDTVHHKIGDLQSAVAGLARGDRQELCIASVPSI
                     ANVMLPRAIAALRRRYPDLALDISILKIEEAIDYLLLGRGELVAISSRFDHPLIGFEH
                     LASGRLLCIVPENGPLAARERITPAEMAEFPLIGINPRDPYGAVMAAMFK"
BASE COUNT          375 a          675 c          640 g          369 t
ORIGIN      
        1 cgcggaaaac agcggatcga tgcggaccgt gccggtgaac cagtccgccg accctttgtt
       61 ggacgggcgg gagcccgcgc gttgaacatt catggaagta tcctttctgt cgctggtcga
      121 tatgttcggc gctggtcgtc ggttccggcg cctgggcgcc aggtcgccgc cgcgatggca
      181 aatacggcag ttcgtcagag attcgagcct tcggccagtt tggtgtcgta ggttgtttcc
      241 atggatggac cgcttccttg ccgcgcttga ggcggcggcc cagattgagg gccgatcggc
      301 tttcttgcgg cgccgatccg cgctcatcca tatagaccat cgcaatccgc atgattattc
      361 ggcgcaattc gcatggactt atcgatccgt gatataagtc cgccggaggt atccgatgga
      421 tcgactgaat ctgaacgaca tgctgctgtt catggccgtc gccgaggaac gcagcttcac
      481 cagggcggcc gcgaagctgg ggacatcgca gtcgacgctc agccatacga tcaagcaact
      541 cgaggcacgg ctcggcctgc ggctcctgac gcgaacgacg cgcagcgtct caccgacgga
      601 agccggcgag cggctcctgc attccctggc gccgcgcatc cacgagatcg agagcgatgt
      661 cgacgcgttg atggcgtttc gcgacaggcc ctcggggacg gtgcggatca cgctctccga
      721 tcacgcgctg gactggatcg tctggccgaa gctgaagccg gttctgcggg actatcccga
      781 catccacgtc gaactgaacg tcgataacgg cttccgcaac atcgtcgagg ggcgcttcga
      841 cgccggcgtg cggctgggcg aaagcgtgga caaggacatg atcgccatcc gcatcagccc
      901 ggactggcgt ctcgtggccg tcgcctcccc cgaatatttg gcgctgcaca agacgcccga
      961 ggcgccgcag gacctggtcg gccacaattg catcaaccat cgccagccgc aggcgggcgg
     1021 cctctacgcc tgggaattcg aaaaggacgg acgggagttg cgagtgcgag tcgacgggca
     1081 gctgacgttc aacacatcca tcgccatggt cgatgcagcc acaggcgggc ttgggatcgc
     1141 ctatgtgccg gagagcctgg tcatggaccc gatcaggtcc ggccgcctcg tgcggctgct
     1201 cgacgaatgg tgcccgaaat tcccgggcta tcatctctac tatccgactc ggcggcagaa
     1261 ctcgcctgcc ttcgcggtta tcgtcaatgc gctgcgccat acggccagga cggacacccg
     1321 ctgaccagac acaaccggct cgacgaaatt ccctcgaagc aaaatcgcat gtaagcaaga
     1381 aagcctcgcg tccggagacg cgacaggact gtccccaggg cgttcaaatg cttacactgc
     1441 gccagatcga agtcatccgc gccatcatgg tgacggggag catcgccggg gccgcgcggc
     1501 tgctcaacgt cgccgcgccc ggcatcagcc ggctcatgaa atactccgag gattctctcg
     1561 gcgtgcgtct gttcgaccgg cgcgccggac gctacgtgcc cacgccgcag gcgcgccaca
     1621 tcttcgacct gctggacacc gtccatcaca agatcgggga tctgcagtcg gcggtcgccg
     1681 gactggcgcg cggcgaccgc caggagctct gcatcgcatc ggtgccgagc atcgccaacg
     1741 taatgctgcc gcgcgccatc gcagcccttc gccggcgcta tcccgacctc gccctggaca
     1801 tcagcatcct caagatcgag gaggctatcg actatcttct cctcggtcgg ggagagctcg
     1861 tcgccatctc ctcgcgcttc gatcatccgc tgatcgggtt cgagcatctc gccagcggca
     1921 ggctgctctg catcgttccc gagaacgggc cgcttgccgc gcgcgaacgc atcacaccgg
     1981 cggagatggc cgagtttccc ctaatcggga tcaatccaag ggacccgtac ggcgcggtca
     2041 tggccgccat gttcaagga
//
LOCUS       DASSGX010000185         3141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1124355, whole genome shotgun sequence.
ACCESSION   DASSGX010000185 DASSGX010000000
VERSION     DASSGX010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1124355"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..1387)
                     /locus_tag="VFI12_03270"
     CDS             complement(86..1387)
                     /locus_tag="VFI12_03270"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7055453.1"
                     /translation="MKRQAGLIIAALLVAAGCSSTPGSSAPASASGSPPAASAAVQDG
                     GTIKVGFVGNLTGQIANIGQDALNASQLAADTVNAQGGIVVGGKRYTITVVSEDEGSQ
                     VNDPGTAVSAVNRLISGDVDAIVGGVVTSNVLAFMPVTQSAGVPLIDTIGRGQSIIDG
                     IAKDKLDTVFVASATTRDAAAGIAGAMKNLIAPTKVAYILVDTDATHGLLADIQAANA
                     AAGLTVQEQVTFVPQGTTDFASYILKIKDFAPDVIYADVVGGSLAWARQLSQSGYTTG
                     YVVIGDAEYVQADFLSQASTEANLSLVVSVTVPSSVTPVAEAFYKDYAAKFGAPASNY
                     AVQQYDGMLALFEGIRRSGGLTGDVAADRAAIVKGLAQITEAAPFVGIRSGTAFFSSL
                     ETGHKMPANAVITQIQDGKFVPVWPADVAQAAGGTFIDPRK"
     gene            complement(1384..1812)
                     /locus_tag="VFI12_03275"
     CDS             complement(1384..1812)
                     /locus_tag="VFI12_03275"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET7055454.1"
                     /translation="MSTSDRPAQGNPLDEVLRRRGYVLPVHETLARIDPELLQRYEDF
                     AGEILFSDKPKALPLMVRYLVLVGITTAVRGDAEGVEWSGTRAMQNGATEEQVLEAMA
                     LAMLPAGVPAFEEAVKAWKGVLDGDSLIQRRSVNKEETQK"
     gene            complement(1902..2642)
                     /locus_tag="VFI12_03280"
     CDS             complement(1902..2642)
                     /locus_tag="VFI12_03280"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HET7055455.1"
                     /translation="MQSVSRFAKILSCFTRDTPVLGITQISTQLGLPKTVVHRILGAM
                     ADAGLVEHAAGSTKYRLGPAAIEMAFAAIGSPDIREVAMPVMVELQQRTGESVTLCVR
                     SGIERTFIAQVESKFPVRMSVEIGQRYPLYAGASGKSILAQFPPEALDDYLANVALAP
                     ITSNTITDPTALRHELTQIQHDGFAVSHGERDQWAASVAAPLFAGNGLVIGSMSVCGP
                     RPRFEGYDLKRIGRVLARATESITSPSK"
     gene            2712..>3141
                     /locus_tag="VFI12_03285"
     CDS             2712..>3141
                     /locus_tag="VFI12_03285"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HET7055456.1"
                     /translation="MHNRWHPAIPAIQTISAGDLVTADLSDGLAGQIGADTSAADLVS
                     LDFTLTHPLTGPFWIEGAEPGDLVGVEIVDIVPGAQGFTFVIPGLGLLPELTGPFLVQ
                     WQLGDGVARSSELPGVEVAGHPFLGVVGVAPSEKRFAQFRL"
BASE COUNT          537 a         1040 c          985 g          579 t
ORIGIN      
        1 gtcgtcgagg gcgaccgtgg cgccgaactg cttggatgcc gcgactaccg agagcacgcc
       61 gggatcaagt cgtttcgcgt accggtcact ttcggggatc gatgaatgtt cctccagccg
      121 cctgcgccac gtctgcaggc cacaccggga cgaacttgcc gtcctggatc tgcgtgatca
      181 cggcgttggc cggcatcttg tgtcccgtct ccaggcttga gaagaacgcc gtaccacttc
      241 ggatgccaac gaacggtgct gcctcggtga tctgcgcgag gcccttgacg atcgccgcac
      301 gatccgctgc cacgtcgccg gtgagacctc cagatcgccg gatgccttcg aacaacgcga
      361 gcatgccgtc gtactgctgt acggcgtagt tcgaggcggg tgccccgaac ttggcggcgt
      421 agtccttgta gaacgcctcg gcaacagggg tcacggagct cgggaccgtc acggagacga
      481 ccagcgagag gttcgcctcg gtcgaggcct ggctgaggaa atcagcttgg acgtactcgg
      541 cgtcgccgat taccacatag ccggtggtat agccggactg gctcagttgc cgtgcccacg
      601 cgaggctgcc accgacgaca tcggcgtaga tcacatcggg tgcgaagtct ttgatcttga
      661 ggatgtacga ggcgaaatcc gtcgtgccct gcggaacgaa ggtcacctgc tcctgcaccg
      721 tcaagccagc ggcggcattg gccgcctgga tgtcggcgag caggccgtgc gtcgcatccg
      781 tatcgacgag gatgtaggca accttcgtcg gagcgatcag attcttcatc gcgcccgcga
      841 tgccggcggc cgcgtctcgt gtcgttgcgc tggccacgaa gaccgtgtcg agcttgtcct
      901 tggcaatgcc atcgatgatg ctctggcccc ggccgatcgt atcgatgagc ggcacgccag
      961 cgctctgcgt gaccggcatg aaggcaagca cgttcgatgt cacaacgccg ccgacgatcg
     1021 cgtcgacgtc gccgctgatc aggcgattga ctgcggatac agccgtcccc gggtcgttga
     1081 cctggctgcc ttcgtcctcg ctgacgacgg tgatcgtgta ccgcttgcca cccacgacga
     1141 tgccgccctg cgcgtttacc gtgtcggcgg ccaattgcga cgcgttgagg gcatcctgtc
     1201 cgatgttggc gatctgtcca gtgagattgc cgacgaaccc aaccttgatc gtcccgccgt
     1261 cctggaccgc cgcggacgcg gctggtggac tacctgacgc ggatgcgggc gccgacgaac
     1321 cgggcgtgct cgagcatcca gcagccacga gtagcgcggc aatgatgagc cctgcctgtc
     1381 tcttcatttc tgggtctcct ccttgttcac gctccgacgc tgaatcaggc tgtcgccatc
     1441 gagaacgccc ttccaggcct tgaccgcctc ctcgaaggcg ggaacgccgg ccggaagcat
     1501 cgccagcgcc atcgcctcga ggacctgctc ctctgtcgca ccgttctgca tggcccgagt
     1561 ccccgaccac tcgacaccct cggcatctcc tcgaacggca gtcgtgatgc cgacgagcac
     1621 caggtatcgg accatgagcg ggagggcctt aggtttgtcc gagaagagga tttcgccggc
     1681 gaagtcctcg taccgctgga gcagctcagg gtcgatccgc gcgagcgtct cgtgcaccgg
     1741 aagcacatag ccacggcgac ggagaacctc gtcgagtgga ttgccttgcg caggtcgatc
     1801 agatgtactc atcgccgcca aggtacgtgg cagcggtccg gatggaaccg agacgttccg
     1861 ctcagcggga tccttgggtt gcgcgaacgg gctcctgccc tctatttcga gggtgacgtg
     1921 atcgattcag ttgcccgcgc caggacacgg ccaatccgct tgaggtcata cccctcaaat
     1981 cgtgggcgcg gtccacacac gctcatcgaa ccgatgacca aaccgttgcc cgcgaacagc
     2041 ggcgcagcga cagaggccgc ccactggtcc cgctccccat ggctcacggc gaacccgtcg
     2101 tgctggatct gagtcaactc gtggcgaagc gcagtcgggt cggtgatggt gttcgaggtg
     2161 atcggtgcga gtgcgacgtt tgcgaggtag tcatccagcg cttcgggagg gaattgggcc
     2221 aggatcgact tgccagacgc cccggcatac aacggatagc gctgaccgat ctccacggac
     2281 atccgcaccg gaaacttgct ttcgacctgg gcgatgaacg ttcgctcgat ccccgagcgg
     2341 acgcagagcg tcaccgactc tccggttcgc tgctggagtt ccaccatcac cggcatcgcc
     2401 acctcgcgaa tgtcgggcga accgatcgcc gcgaaggcca tctcgatcgc ggccgggccg
     2461 agccgatatt tcgtcgagcc ggcggcatgc tcgacgagtc ctgcgtccgc cattgcgccc
     2521 aggattctgt ggaccaccgt cttcggcagc ccgagctggg tcgagatctg ggtgatgccg
     2581 agcaccggcg tatcgcgggt gaagcacgaa aggatctttg caaagcgact taccgactgc
     2641 atcgactgtc caccgcacct tcaacgccgc ccatcgatca tccatcctga ctctgggagc
     2701 actcgaccgc catgcacaac cgatggcacc ccgcgatccc cgcgatccaa acaatatccg
     2761 cgggcgacct cgtcaccgcc gatctctcgg acgggctggc tggccagatc ggcgcggaca
     2821 cctccgccgc agacctggtg tcgctcgact tcaccttgac gcatccgctc acgggcccgt
     2881 tctggatcga aggagccgag ccgggcgacc tcgtgggggt cgagattgtc gacatcgtcc
     2941 ccggagcgca aggcttcacc ttcgtgattc ccggcctcgg tctgctgccc gagcttaccg
     3001 gaccgttcct ggtgcagtgg cagctcggcg acggggtcgc ccggtcaagc gagctgccag
     3061 gggtcgaagt ggccggccac ccgttcctag gcgtcgttgg tgtcgccccg tccgagaaac
     3121 ggttcgccca gtttcggctt c
//
LOCUS       DASSGX010000186         3824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_362211, whole genome shotgun sequence.
ACCESSION   DASSGX010000186 DASSGX010000000
VERSION     DASSGX010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362211"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1154)
                     /locus_tag="VFI12_03290"
     CDS             complement(<1..1154)
                     /locus_tag="VFI12_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HET7055457.1"
                     /translation="MTRSYDIVVIGTGMGGGTLARALMDNGLKILLLERGPMLPVEPQ
                     NQSAAALFIENRYKARELWIDDKTGSPYKPGVHYWVGGNTKVFGAAFPRLRPEDFDAL
                     EHEGGTSPAWPISYADLEPYYAIVERWYQVHGEAGVDPTEGWRSTPYPHPPVPADPYM
                     DDLAGRMRQNGLHPFPLPIGIDLRTGGKCIRCGTCDAFPCMYDAKGDADVCGVRPALA
                     SGNVELETGAYVRRLLTDKRGKKVTGVETVRDGTFETITAGTVVVACGAVNSSALLLR
                     SANDAHPRGLANRSDQVGRNYMVHNNAVLIAIDPRRSNPTIFQKSMAINDFYFSGPDY
                     PYPLGNLQPVGKIQTPMLKSTVPRVPDFVLGPIASHSTDWWVMSEDLPDP"
     gene            complement(1151..2014)
                     /locus_tag="VFI12_03295"
     CDS             complement(1151..2014)
                     /locus_tag="VFI12_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET7055458.1"
                     /translation="MAADLSGLRAAHPRQKRSFGNIVVFVVLVLAALFYVAPVYVMII
                     NGLKDKSYMTLSDMWKLPRDLSGGGFGYAWDALRPYLANSLKMAIPATVGSSLLGAVN
                     GYLLSKWKFRGSDVLFSLLLFGMFIPYQSILVPLIETLQKFHLYGKWYGLTTVHIIYG
                     IPITTLIFRNYFSNIPNELIEAARIDGAGLIKIFWQIMLPLGLPAFVVVGIFQFTNIW
                     NDFLFGVTVVFSPSAQPVTVALNNLQGTQSVDWTVVMAGAAIAAAPTMIIYVLLGKFF
                     IRGLLAGSMKG"
     gene            complement(2014..3054)
                     /locus_tag="VFI12_03300"
     CDS             complement(2014..3054)
                     /locus_tag="VFI12_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET7055459.1"
                     /translation="MATLTTAASVERTGAQARRRRGAGERWLSPALLSPSIVAMFIFV
                     YGFIVFTFWVSISNWKTNRRDLTVSDPWYKTYDTLFNQTRFQIDLRNTFVFTVLFLIL
                     AVVAGLALAILLDRHVMGSNLFRSIFLFPYAISFITTGVVWRWIFNPETGVNALFDQT
                     GINDALGKMGVGPLKPGWITDPAVIWQVNGALEKIFPWADGIKANLGIPVAMIPIVIA
                     ATWQLSGFAMAMYLAGLGTIPGEIKEAALVDGASDWQAYKDIIIPLLKPITISTMIIL
                     GHVSLKIFDLIYAMSGKGPGFATDVPGIFVYDKLFGAQQYNLGSAASIVMLVLVSLVI
                     VPYLWRQLKELS"
     gene            complement(3142..>3824)
                     /locus_tag="VFI12_03305"
     CDS             complement(3142..>3824)
                     /locus_tag="VFI12_03305"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET7055460.1"
                     /translation="GLAVGDKDGFVDAQTFENHLLGALGPDKYVSLFSGESAWDDQEV
                     KDVMTSFTKLLDYINSDHSALSWSDATAMIIEGKAAFNTMGDWAYGEFVAKNVTDKMG
                     WLSYPGSDGSFVLVVDGFTKPKGAPNMDNAIAWLKTCGSVEAQAAFNPLKGSIPARTD
                     VPTDGFSEYHKWSIASFAKDHLVPSAAHGLATSPAFKQAFYDAAIAFMTDKDVDTFAT
                     TLADAASA"
BASE COUNT          756 a         1163 c         1218 g          687 t
ORIGIN      
        1 tcggggtcgg gcaagtcttc gctcatcacc caccagtcgg tactgtggct ggcaatcggc
       61 ccgagcacga aatccggcac gcgcgggaca gtgctcttca gcatcggtgt ctggattttg
      121 ccgaccggct gcagattccc gagcgggtac ggataatccg ggccggagaa gtagaaatcg
      181 ttgattgcca tgctcttttg gaagatggtc gggttggacc ggcgcggatc aatcgcgatc
      241 aggaccgcgt tgttgtggac catgtagttc cgcccgacct ggtccgacct gttcgccagg
      301 ccacgcggat gagcatcgtt cgccgaccgc agcaacagcg ccgacgagtt gaccgccccg
      361 catgcgacga cgaccgtgcc ggcggtgatc gtctcgaagg taccgtcgcg gaccgtctcg
      421 acgccggtta ccttcttgcc gcgcttgtcg gtcagcaggc gacggacgta cgcgccggtc
      481 tccagctcga cattacccga tgcgagtgcc ggccgcaccc cacagacatc ggcatcgcct
      541 ttcgcgtcat acatgcacgg gaatgcgtcg caggtaccgc agcggatgca tttgccgccg
      601 gtccggagat cgatcccgat cggcagcgga aacgggtgca atccgttctg gcgcatgcga
      661 cccgccaggt cgtccatgta cggatcggcc gggaccggcg gatgcggata cggagtcgac
      721 cgccagcctt cggtcgggtc gactccagcc tcgccatgca cctgatacca gcgttcgacg
      781 atggcatagt acggctcgag gtccgcgtac gagatcggcc acgccggcga ggtgccaccc
      841 tcatgctcca gcgcatcaaa gtcctcgggc cggagcctgg gaaaggccgc gccgaagact
      901 ttcgtgttgc caccaaccca gtagtggacg cccggcttgt acggagagcc ggtcttgtcg
      961 tcgatccaga gctccctggc cttgtaccgg ttctcgatga agagggcggc cgcgctctgg
     1021 ttctgcggct cgaccggcag catcgggcct cgctcgagca acaggatctt cagcccattg
     1081 tccatgagcg cccgggcgag tgtcccaccg cccattccag tgccgatgac gacgatgtcg
     1141 tacgaccggg tcatcccttc atcgatcccg ccagcaagcc gcgaatgaag aacttgccga
     1201 gcagcacgta gatgatcatc gtcggcgccg ccgcgatcgc cgcgccggcc atgaccacag
     1261 tccagtccac cgattgcgtg ccttgcaggt tgttgagggc cacggtaacc ggctgggccg
     1321 atggcgagaa gaccacggtt acgccgaaca ggaagtcgtt ccagatgttg gtgaattgga
     1381 agatgccgac taccacgaag gccggcaaac cgagtggcag catgatctgc cagaagatct
     1441 tgatcagccc ggcgccgtca atccgggccg cctcaatcag ctcgttcggg atattcgaga
     1501 agtagttgcg gaagatcagc gtcgtgatcg ggatgccgta gatgatgtgg actgtcgtca
     1561 ggccgtacca cttgccatac aggtggaatt tctggagcgt ctcgatcagc ggcaccagga
     1621 tgctctggta cgggatgaac atgccgaaga ggaggagcga gaagagcacg tccgatcccc
     1681 ggaatttcca cttcgagagc aagtacccgt tgaccgcgcc gagcagcgac gagccgaccg
     1741 tggccgggat cgccatcttc aggctattgg ccagatacgg ccggagcgca tcccaggcat
     1801 aaccgaaacc gccgccggag aggtcgcgcg ggagtttcca catgtccgaa agggtcatgt
     1861 agctcttgtc cttgagccca ttgatgatca tcacgtagac gggcgccaca tagaacaatg
     1921 cagccaggac cagcacgaca aagacgacga tattgccgaa gctgcgcttt tggcgcgggt
     1981 gcgcggcacg aaggccgctc aggtcggcag ccattacgac agctccttca actggcgcca
     2041 gaggtagggc acgatcacca gcgagacgag aaccagcatc acgatcgacg ccgccgatcc
     2101 gaggttgtac tgctgggcgc cgaacagctt gtcgtagacg aaaatgccgg gcacgtcagt
     2161 cgcgaacccg ggacccttgc cggacatggc ataaatcaga tcgaagattt tcagggagac
     2221 gtgaccgagg atgatcatcg tgctgatcgt gatcggcttg agcaacggga tgatgatgtc
     2281 tttgtaggct tgccagtcgg acgcgccgtc gaccagcgcc gcttccttga tttcgccggg
     2341 aatcgtgccg agcccggcca ggtacatcgc catcgcgaag ccggagagct gccaggtcgc
     2401 cgcgatcacg atcgggatca tggcgaccgg gatgccgaga ttggccttga tgccatcggc
     2461 ccacgggaag atcttttcca gcgcgccgtt cacctgccag atgaccgccg ggtcggtgat
     2521 ccagcccggc ttgagcggcc caacgcccat cttgccgagc gcgtcgttga tgccggtctg
     2581 atcgaagaga gcgttgacac cggtttccgg gttgaagatc caccgccaga cgacgccggt
     2641 cgtgatgaac gagatcgcgt acgggaacag gaagatgctc cggaacaggt tgctgcccat
     2701 gacgtgccgg tccagcaata tggcaagcgc aagccccgcg acaaccgcga ggatcaggaa
     2761 cagcacggta aagacgaacg tattgcgaag atcgatctgg aagcgggttt ggttgaacag
     2821 ggtgtcgtag gtcttgtacc agggatcaga tacggtcaga tcgcggcggt tcgtcttcca
     2881 attcgaaatc gatacccaga acgtgaagac gatgaacccg tagacgaaga tgaacatggc
     2941 cacgatcgac ggcgagagta acgcgggtga gagccagcgc tcgccggcgc cacgccggcg
     3001 ccgtgcctgg gctcccgttc gttcaacgct cgctgcggtg gtcagagttg ccatggatcg
     3061 ccgtccgctc cacctcgact gaaacgcacc ggggcgcacg agtgtgcgcc ccggttgtca
     3121 tcgaaagaac tcgacgccga cttaggccga ggcggcgtcc gcaagcgtgg tcgcgaaggt
     3181 gtcgacatcc ttgtcggtca tgaacgcgat tgccgcatcg tagaaggcct gcttgaaggc
     3241 cgggctggtg gcgagaccgt gcgcggcgct cggcaccagg tgatccttcg cgaacgaagc
     3301 gatcgaccac ttgtggtact cggagaagcc gtctgtcggg acatcggtgc gggccgggat
     3361 cgaacccttg agcgggttga aggccgcctg cgcctccacc gaaccgcagg tcttgagcca
     3421 ggcgatcgcg ttgtccatgt tcggcgcgcc cttgggcttg gtgaagccgt ccacgaccag
     3481 tacgaacgag ccgtctgagc ccgggtagct gagccagccc atcttgtcgg tgacgttctt
     3541 ggccacgaac tcgccgtatg cccagtcgcc catcgtgttg aatgcggcct tgccctcgat
     3601 gatcatcgcc gtcgcgtcgg accaactcag ggccgaatga tcggagttga tgtagtcgag
     3661 cagcttggtg aaggaggtca tgacatcctt cacttcctga tcgtcccaag ccgactcccc
     3721 gctgaacagg gaaacgtatt tatccgggcc cagcgcgccg agcaggtggt tctcgaaggt
     3781 ctgggcgtcg acgaaaccgt ccttgtcgcc aacggccagt ccgg
//
LOCUS       DASSGX010000187         2603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1125330, whole genome shotgun sequence.
ACCESSION   DASSGX010000187 DASSGX010000000
VERSION     DASSGX010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1125330"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VFI12_03310"
     CDS             <1..389
                     /locus_tag="VFI12_03310"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HET7055461.1"
                     /translation="PNGVAFNADETAVYLAETYHTRILRYEIRSDGSLGPRDVWGQTG
                     DPAGPDGMAFDAAGNLYCAHHGGSRVDIFNPDGERIGDIPIPGANVTNCAFGGPDRKT
                     LVVTEVETASLYMVGMPAPGQLLNDR"
     gene            complement(603..1313)
                     /locus_tag="VFI12_03315"
     CDS             complement(603..1313)
                     /locus_tag="VFI12_03315"
                     /inference="COORDINATES: protein motif:HMM:NF015658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HET7055462.1"
                     /translation="MLVPIALLFVLVMPALVAAQATPDAQTAEAVCGRIAGTPVGTPT
                     TVDLSVGDLQALDLDLIFLDAMIPHVQASMDMATIARDRSARPEVVEFASAIISGQQP
                     QVEAMVAWRAEWYPDVPELSWLQLVDAMNMKLSESPGVGGVAGLEAMDPTHRTADLAS
                     LCGSGDIDLAFIDALIAHNGSSIILAKATIDRSTHREVREIGGSLATTQQYEIDQLLA
                     WRVAWFAGTPIPDDHSGD"
     gene            1601..>2603
                     /locus_tag="VFI12_03320"
     CDS             1601..>2603
                     /locus_tag="VFI12_03320"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET7055463.1"
                     /translation="MADRDQTIEQLYQEAVTKRLNRRAILARGMALGLSLPAIGSVAA
                     MAQDASPAASPAPMTSPAQNGPVHVPIVDKDMSFDDIKAAIADEKEVTVGNWTYTAND
                     QLVKRFQDYVKTVYGEDIKLNYIGSQSPSTYIADLYTTAAAGGDSAYDVMAIEENYWA
                     QVQSDAKTQNTTFMQDFLPSGLIPNAERVMDNLKHVPTSVGFQASATPGLNYNKAKVD
                     FLKDWKDLADERLKGKVLLWLPGDITGGGMLLGMCDSLGLDYHNPDHVTQAIDFIIDK
                     IGPNVLKYTSDNAEAQSLFTSGAVDVVAFWNSMARLQYLNGMKDAAFLVAASGQYAVN
                     "
BASE COUNT          499 a          897 c          772 g          435 t
ORIGIN      
        1 tcccgaacgg cgtcgcgttc aacgcggatg agacggccgt ctacctggcc gagacctatc
       61 acacacggat cctgcggtac gagatcaggt ccgacggctc gctcggtccc cgcgacgtct
      121 gggggcagac cggcgacccg gccggaccag acggcatggc cttcgacgcc gcgggtaacc
      181 tctactgcgc gcatcacggc ggcagccggg tcgacatttt caacccggac ggcgagcgga
      241 tcggcgacat cccgatcccc ggcgcgaacg tcaccaactg cgccttcggc ggcccggacc
      301 gaaagacgct ggtggtaacc gaagtcgaaa ccgcctcgct ctacatggtc gggatgcccg
      361 cccccggcca actcctcaac gaccgatagc atctgcggtt tggtcgagtc cgggtagtcc
      421 tctcccagaa cgtagcgcgg ggttaccatg ccccacccgg gcccggtgaa gcccgcgccg
      481 tcagagggaa ccaatttcgg taccggctcg gctccgccaa acgcgcgagg gatgtaagtt
      541 cggggcatgg taaccccgcg ccccgtttcg ttgaacgtct cccgctaaat caagtcggaa
      601 actcagtcgc ccgagtgatc gtcgggaatt ggggtgccgg cgaaccaggc cacgcgccag
      661 gcgaggagct ggtcgatctc gtactgctgt gtggtggcga gactcccgcc gatttcccgg
      721 acttcccggt gcgtggagcg gtcgatcgtt gccttggcga ggatgatgct gctgccgttg
      781 tgggcgatca gcgcgtcgat aaaggccagg tcgatatccc cgctgccgca caggctcgcg
      841 agatcggccg tccggtgcgt cggatccatc gcctcaagac cagccacgcc gcccacgccc
      901 gggctctccg acagcttcat gttcatcgcg tcgaccagtt gcaaccaact cagctccggc
      961 acgtccggat accactccgc gcgccaggcg accatcgcct cgacctgcgg ctgttgaccc
     1021 gagatgatcg cactggcgaa ctcgacgacc tcgggccgcg cactgcgatc ccgggcaatc
     1081 gtcgccatgt ccatcgatgc ctgcacgtgc gggatcatcg cgtcgaggaa gatcaggtcg
     1141 agatcgagcg cctgcaagtc gccaaccgac agatcaaccg tcgtcggtgt ccccaccggc
     1201 gtacccgcaa tccggccaca aaccgcctca gcagtctgcg cgtcaggcgt tgcctgcgca
     1261 gccaccaacg ccggcatcac cagcacaaaa agcagggcaa ttggaaccag cacgcgtcga
     1321 ttcatccaac aattgtaggg gcagatggca tctgcccgag actccccaaa gccgcatctc
     1381 gccggtaacc cggttcggca aaaagccggt atctgtgatc gggccttgcc gacgcggcgg
     1441 gtttgccccc gcaaactcct acattggttc cctcaacccg cctccccgcc tgttctccgc
     1501 gctcttgaca cttattccgc accctcctac tgttccggaa gtctcaccgc tgtcggtttt
     1561 gcgccacgcg ggccaaccgg cctgcaggag gatttgcacc gtggcagatc gtgatcagac
     1621 catcgagcag ctctaccagg aggccgtgac gaagcgattg aaccgtcgcg cgatcctcgc
     1681 ccgcggtatg gcgctcggac tttcgctccc cgccatcggc tcggtcgcgg cgatggcgca
     1741 ggacgcctcc ccggctgcca gcccggcgcc gatgacctcg ccggcccaga acggaccggt
     1801 ccatgtcccg atcgttgaca aggacatgtc cttcgacgac atcaaggccg caatcgccga
     1861 cgagaaggaa gtgacggtcg gcaactggac ctacacggcc aatgaccagc tcgtcaagcg
     1921 cttccaggat tacgtcaaga cggtctatgg cgaggacatc aagctcaact acatcggctc
     1981 gcaatcgccc agcacctaca tcgccgacct ctacaccacg gcggcggcgg gcggcgattc
     2041 cgcctatgac gtgatggcga tcgaagagaa ttactgggcg caagtgcaat ccgatgccaa
     2101 gacccagaac accacgttca tgcaggattt cctgccgtcc ggcctgattc cgaatgcgga
     2161 acgcgtcatg gacaacctga agcacgtacc cacgtcggtc gggttccagg cgtcggcgac
     2221 cccgggcctc aattacaaca aggcgaaggt cgacttcctc aaggactgga aggacctcgc
     2281 cgacgagcgg ctcaagggca aggtcctgct ctggctgccg ggcgatatta ccggcggcgg
     2341 catgttgctc ggcatgtgcg attcgctcgg actcgattac cacaacccgg accacgtgac
     2401 ccaggcgatc gacttcatca tcgacaagat cggcccgaac gtgctgaagt acacatcgga
     2461 taacgccgag gcccagagtc tcttcaccag cggcgccgtc gacgtggtcg cgttctggaa
     2521 ctcgatggcc cgcctgcagt atctgaacgg gatgaaggac gccgccttcc tggtagcggc
     2581 ctccggccag tacgcggtca acg
//
LOCUS       DASSGX010000188         6008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_797981, whole genome shotgun sequence.
ACCESSION   DASSGX010000188 DASSGX010000000
VERSION     DASSGX010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6008
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797981"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /locus_tag="VFI12_03325"
     CDS             <1..935
                     /locus_tag="VFI12_03325"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7055464.1"
                     /translation="PGEVPVMTARGAMLTPGDEAEYRPGLPEPKIVSQRRRQLRSFAK
                     NKPAVVGLALILLIVVIALAAPLIAPHDPLDQSVRNRLAAPNSDYLLGRDDKGRDILS
                     RVIYGTRIALKVGIFSVLLGGTLGTAIGVVAGYFGGKTDTVLMRLTDVLLAFPDLITG
                     LLVLAVLGPGLWKMVLAIGLTITPRFARIAYGPTLSVKQTEFVEAARSVGVGNGRILS
                     RHILPNVGGDLVVFGSLWTASAIRLEASLSFIGLGVPPPTPTWGQMIREGTIHLTAVP
                     LYSLGPGIALLITVFAFNLVGDGLRDALDPRAAT"
     gene            complement(1004..1225)
                     /locus_tag="VFI12_03330"
     CDS             complement(1004..1225)
                     /locus_tag="VFI12_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055465.1"
                     /translation="MHATNATWSLRNIASGALIALTLATGCLIGAVESASAQPPTLRE
                     ERGHSPHAVAAATQDPDPAYHHARIGFLP"
     gene            complement(1382..3688)
                     /locus_tag="VFI12_03335"
     CDS             complement(1382..3688)
                     /locus_tag="VFI12_03335"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET7055466.1"
                     /translation="MTTAPRRFQLAPLPAPRTPLIGRGRELADVSRLLADPASSLVTL
                     TGPGGIGKTRLALAVAHKPQQQFEDGVAFVRLSQLRDPALALPTIAQTLSVPETGAAP
                     IADLVRDAIGTSHLLLVLDNFEHVAVAGADLGGLLDMCPNLTLLITSRVPLRLSGERI
                     YSVPPLAVPASSRVTSRVVAESEAGNLFASRAAAVRSGFAISDENATHVAAICQHLDG
                     LPLAIELAAARINILSPAALLARLDRRLPLLTGGPRDLPERQHTMRNAIAWSYDLLPE
                     AEQRLFRRLSVFDGGFSLDAAEAVTAGDPTIDALAGVSTLVDASLIRPIDDPSGEPRY
                     LMLETIREFGLECLTENGDEKAARDLHARHILGLVQTGNELRDTQRASDWLVRLTSEI
                     ANIRAARSWFETTGNVHAGMSLADGASWIWDWRTLFVEGRDWLSQSLSAAPNAPPGVR
                     ASAMVGAGWMAFRQLDFADAQLSATAGLTLARAAGDHRVAAELLDLLGKVALETGQAQ
                     DARACFEEALALTHAYPLEHYESAAINSLGIVAFTMGDLAEAQRCHEASVTIDRRIGH
                     RLSLANHLFALAMTVFCRGALAHAQEVLQEQLRAIIDLDLGIEFGCQIVAMFAANEQP
                     ARAARLFGACEAAADLLGADLFETDAYRPWYESAVARARESLGPDMFDTTMHTGREYS
                     REQVLDEAMAVFAPPSRVEATCAFGLSKREREVLRLVAHGQTNRAIADQLFIAESTVK
                     VHVTAIFTKLGVASRSAATAVAIRNGLA"
     gene            4051..4659
                     /locus_tag="VFI12_03340"
     CDS             4051..4659
                     /locus_tag="VFI12_03340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7055467.1"
                     /translation="MTEREDITTARTRDGRHLEPLAWAESDESVAVRAAADPGAMTEL
                     YRRYIARVARYCRRRLQDPDLAEDVTSQIFLKVLEGLRKRRVENVASWIFTIAHNEVV
                     SQYRRQRELIGITETVLALGDDDQLEETIITQVVAGDLRAMLSRLSADQQRVLELRLA
                     GLTSREIRQVLGRSQTWIGTTEYRAVHRLRDLMTSNYPGEER"
     gene            4660..>6008
                     /locus_tag="VFI12_03345"
     CDS             4660..>6008
                     /locus_tag="VFI12_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055468.1"
                     /translation="MRWPWRYRQSNDARALDEYWDEVVRAAPAMPVPQPDDSPELARV
                     IRQLHAAEEADHSRPAYEDRLLQRLLALQESSVADAVPGGIASSLPSTSIRRDSRVDW
                     RPPSGRRIGTQLSRAAAFALVLVVVLIGAQFANTRWNREDSPAIIAPSAASPTVVAGP
                     HEIPELSGVSLLWSAQVEPTRLARLSDVAVAPDGDIYAVDCKNELILVFSADGNYLES
                     WGGPGTAPGQFKFGKCAFDPLFGDPINSQLLGAIGFDQSGNIYVFDSLNNRIQKFGPD
                     RSFITQWGSFGTEIGQFSRPLGTVDAAHDLVYVADLNNQRIQVFDLDGNFIRSWKTSN
                     LPLVNYFGPLDVAVDSAGNVYVSEWEVHMVQKFDPTGKLLGVIVPKDGPGRSFGIALD
                     AHDNLYMSVHDAAVIDVYRQDGTVIGQIRTAGDGGVFRQPGGLAFDGEGNLLVASGGG
                     "
BASE COUNT         1073 a         1795 c         2057 g         1083 t
ORIGIN      
        1 ccccgggtga ggtaccggtg atgacggcga gaggagcgat gctgacaccg ggggacgagg
       61 cggagtaccg gccggggctg cccgagccga agatcgtctc gcagcggcgg cggcaactgc
      121 gctcgttcgc gaagaacaag ccggcggtag tcgggttggc gctgatcctg ctgattgtgg
      181 tgatcgcgct ggcggcgccg ctgatcgccc cgcatgaccc actcgatcag agcgtgcgca
      241 accggctggc cgcgcccaac tcggactatc tgcttggccg ggatgacaag ggccgggaca
      301 ttctgtcgcg cgtgatctac ggcacgcgga tcgcgctcaa ggtcggcatc ttctcggtgc
      361 tgctcggcgg gacgctgggg acggcgatcg gcgtcgtggc cggctacttc ggcggcaaga
      421 ccgacaccgt cctgatgcgg ctgaccgacg tgctgctggc cttcccggac ctgatcaccg
      481 gcctgctggt gctggccgtg ctcgggccgg ggttatggaa gatggtgctg gcgatcgggt
      541 tgacgatcac gccgcgcttc gcccggatcg cgtacgggcc gacgctctcg gtgaagcaga
      601 ccgaattcgt ggaggcggcg cggtcggtcg gggttggaaa tgggcggatc ctcagccggc
      661 atatcctgcc gaacgtaggt ggcgacctgg tggtcttcgg gagcctctgg acagcctcgg
      721 cgatccggct ggaggcgagc ctgagcttca tcgggctggg cgtgccgccg ccgacgccga
      781 cctggggcca gatgatccgc gaggggacga ttcacctgac ggcggtgccg ctctactcgc
      841 tcggccccgg catcgcgttg ctgatcacgg tcttcgcctt caaccttgtc ggtgacggtc
      901 tccgcgatgc gctcgatccg cgggcagcga cgtgacgaac cgccgggctc cgcgacaagc
      961 accggcggtt cagcacagaa atggtggaag agcggtgctc ctcctagggg agaaagccga
     1021 tcctggcatg gtgataggcg ggatccggat cctgcgtcgc cgctgccacc gcatgaggcg
     1081 aatggccccg ttcctccctg agggtgggcg gctgggctga ggctgattcc acagctccga
     1141 tcaggcaacc ggtggcgagt gtgagggcga tcaatgcccc gctggcgatg ttccgcaggc
     1201 tccatgtggc gtttgtcgcg tgcattaggt tgctcctttc gccgaggatt gccctgggtc
     1261 gcgggtaggc tcttcggttc ctcgggtacg ggagccatgc tggacgtcgg gaacgcccgg
     1321 tacatcgact gaaggtcgta tcgggagggg aggagcggaa atacgacgaa ggtcgtagtc
     1381 ctcaggccaa cccgttgcga atggcgacgg cggtggcggc ggagcgggag gcgacgccga
     1441 gtttggtgaa gatcgcggtg acgtggacct tgaccgtcga ttcggcgatg aagagctgat
     1501 cggcgatggc gcggttggtt tgtccgtggg cgaccagacg gaggacttcg cgctcgcgct
     1561 tgctgagtcc gaaggcgcaa gtggcttcaa ccctggatgg cggagcgaag actgccattg
     1621 cctcatcgag cacctgctcg cgggagtatt cccgtccggt gtgcatggtg gtgtcgaaca
     1681 tatcggggcc gagcgactca cgggcgcggg ccacggcgga ctcgtaccag ggacggtagg
     1741 cgtcagtttc gaacaggtcg gcgccgagca aatcggccgc cgcctcacat gctccgaaca
     1801 agcgagccgc cctggctggc tgctcattgg cggcgaacat cgctacgatc tggcagccaa
     1861 actcgattcc caggtcaagg tcgatgatcg cccgcagttg ctcctgcaga acctcctggg
     1921 cgtgcgcgag agcgccccgg cagaatacgg tcattgccag cgcaaagagg tgattggcga
     1981 ggctgaggcg gtgacctatg cgccggtcga ttgtgactga cgcttcgtgg cagcgctggg
     2041 cttcggcgag atcgcccatc gtgaacgcga cgatgcccaa gctgttgata gcagcggatt
     2101 catagtgctc caggggatag gcatgcgtca gtgccagtgc ttcttcgaaa cacgcgcggg
     2161 catcctgggc ctggcccgtt tcgagcgcga ctttcccgag caagtccagg agctcagccg
     2221 caacccgatg atcccccgcg gctcgagcca gggtcaggcc ggcagtggca ctcagctggg
     2281 cgtcagcaaa gtccagttgg cggaatgcca tccatccagc cccaaccatg gccgaggccc
     2341 gcacgccggg tggagcattg ggggcggcag acaggctttg gcttagccag tcccggcctt
     2401 cgacgaagag cgtgcgccaa tcccagatcc aggacgcgcc gtccgcgagc gacatgccgg
     2461 cgtggacgtt tccggttgtt tcgaaccagg aacgcgcagc ccggatgttc gcaatctcgc
     2521 tggtaagacg taccagccag tcactcgctc gttgtgtgtc gcggagctcg ttaccggtct
     2581 gaaccagccc gaggatatgg cgggcgtgca gatcacgcgc ggccttctca tcgccgtttt
     2641 cggtcaggca ctcgaggccg aactcgcgaa tcgtttccag catcaggtat cgcggttctc
     2701 cgctcggatc gtcgatcggc cggatgaggc tcgcatcgac cagcgtgctc accccggcaa
     2761 gagcatcgat cgttggatca ccggccgtta cggcctctgc tgcatcaagg ctgaagccgc
     2821 cgtcgaagac tgacaggcgg cgaaagaggc gctgctctgc ttcggggagc aggtcgtagc
     2881 tccaggcgat ggcattgcgc atcgtgtgtt gccgttcagg cagatcacgg gggccaccgg
     2941 tcaggagcgg caggcggcga tccaggcggg cgagcagggc cgcaggactc aggatattga
     3001 tgcgcgcggc ggccaactcg attgcgagtg ggagcccgtc caggtgctgg caaatcgccg
     3061 ccacatgggt cgcattctcg tcgctgatcg caaatccgga ccgtactgcg gcagcccgcg
     3121 aggcgaagag attccccgct tcagactcgg ccacaactcg actggtcacg cgggatgacg
     3181 cgggtactgc cagcggtggg accgaataga ttcgctcgcc ggacagacgc aggggaacgc
     3241 ggctcgtaat caggagcgtg aggttcgggc acatatcaag caggccaccg aggtcagcac
     3301 cagctacggc gacatgctcg aaattatcca ggaccagcag caggtgactc gtgccgatcg
     3361 cgtcccgcac cagatcggcg atcggtgctg ctcccgtctc gggcacgctc aatgtttggg
     3421 cgatcgtggg aagcgccagc gcgggatctc gcaactggga aagccgcacg aacgccacgc
     3481 catcctcgaa ctgctgctgg ggcttgtgcg cgacggccaa ggccagccgc gtcttgccga
     3541 taccgccagg gccggtgagg gtgacgaggg aactagcggg atcggctagc aggcggctaa
     3601 cgtcagccaa ttcccgccca cgaccgatca gcggcgtgcg gggggccggg agtggcgcga
     3661 gttggaaacg tcgcggagcc gtggtcatgg tgtttgcctg tcgatgcggc ttcgatcata
     3721 gccgtttggt caatgtaggg aaatctaaat tcgtcagacg ggcccgcgac gaccggctga
     3781 ccgcctctgg gtcgtgattc gggtaatagg gcgacaagtc tcactcgcat atgctgcgaa
     3841 cgcgactgag ataccgctat gtcgcggctg cgggtttagg ttcgtggcga tcctcgtact
     3901 ccacctgcac ctgcggaagc acgcctccaa atagtggtac aacttcgctt attcccgacg
     3961 aatcgcaacc atgccgcgct cgcacacgga gcgcccggat ctgtgcacaa aatcccactg
     4021 ctgcgtctac ctatatgcag gtgagacggc atgacggaac gcgaggacat cacaacagcg
     4081 cggacgcggg atgggcgaca tctcgagccg ctggcgtggg cggaatccga tgagtcggta
     4141 gcggtccgcg ccgccgctga tcccggtgcg atgaccgagc tctaccggag gtacatcgcc
     4201 cgggtcgccc gctactgccg ccggcgtctc caggacccgg atctggcgga ggacgttacc
     4261 agccagatct tcctgaaggt gctggagggg ttgcgcaagc gccgtgtgga gaacgtcgcg
     4321 tcatggatct tcacgatcgc ccacaacgag gtggtcagcc agtaccggcg ccagcgtgaa
     4381 cttatcggga tcaccgagac ggtattggct ttgggtgatg acgatcaact cgaagaaacc
     4441 atcatcacac aggtcgttgc cggagatctg cgggcaatgc tatcgcggct gagcgcggac
     4501 cagcagcggg tgctcgagtt gcggttggcg ggactgacca gccgcgagat ccgccaggta
     4561 ctcgggagga gccagacctg gatcggcacg acagagtacc gggcggtgca tcgtctgcgc
     4621 gatttgatga catccaacta tcccggagag gagcgctaga tgcgctggcc gtggcgatac
     4681 aggcaatcca acgacgcgcg tgccctggac gagtactggg acgaggtcgt tcgcgccgca
     4741 ccggcgatgc cagtgccgca gccggacgat tcccctgaac tggctcgagt cattcgccag
     4801 ctgcacgcgg ccgaggaggc tgatcacagc cgccccgcct atgaggatcg actgctccag
     4861 cggctcctgg ctttgcagga gagttccgtc gcggatgcgg tacccggcgg catcgcatca
     4921 tcgctgccat ccacttcgat cagacgagat tcacgagtcg actggcgacc accatccggg
     4981 cggaggatcg gtacccagct ctccagggct gccgcattcg cgctggtgct ggtcgtggtt
     5041 ctgatcggcg cgcaattcgc caatacgcgc tggaaccggg aggattcccc cgcgattatt
     5101 gcgccttccg cagcgtcgcc aaccgtagtt gcggggccgc acgagattcc ggagttgtcc
     5161 ggcgtctccc tactgtggag cgcacaggtt gaaccgacgc gcctggcaag actgtccgat
     5221 gtggccgttg cgcccgatgg cgacatctat gcggtcgatt gcaagaacga gttgatcctg
     5281 gtcttctcgg cggatggcaa ctacctggaa agttggggcg ggccgggcac agcccctggg
     5341 caattcaagt tcggtaagtg tgccttcgat ccgctctttg gggatccgat taattcccaa
     5401 ttgctcgggg cgatcgggtt cgatcagagc ggcaacatat acgtcttcga ctcgctgaac
     5461 aaccggatcc agaagttcgg gccggaccgc tcgttcatta cgcaatgggg gtcgttcgga
     5521 actgaaattg gccagttcag ccgtccgctc ggaacagtcg acgcggcgca cgatctggtc
     5581 tacgtcgccg acctgaacaa ccagcggatc caggtattcg atctcgatgg gaacttcata
     5641 cgaagctgga agacatcgaa cctgccgctc gtgaactatt tcggacctct cgacgtcgcg
     5701 gtcgattcgg ctggcaacgt ctatgtttcc gagtgggagg tgcacatggt gcagaagttc
     5761 gacccgaccg gcaagttgct gggtgtgatc gtgccgaaag atggaccggg acgatcgttc
     5821 gggatcgccc tcgatgcgca tgacaacctg tatatgtcag tgcatgacgc ggccgtgatc
     5881 gatgtctacc ggcaagacgg gacggtgatc ggccaaatcc gtacggccgg cgatggcgga
     5941 gtgttcaggc agccaggcgg tttggccttc gacggggagg gaaacttgtt ggtcgcaagt
     6001 ggtggcgg
//
LOCUS       DASSGX010000189         3924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1015007, whole genome shotgun sequence.
ACCESSION   DASSGX010000189 DASSGX010000000
VERSION     DASSGX010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3924
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015007"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            380..1978
                     /locus_tag="VFI12_03350"
     CDS             380..1978
                     /locus_tag="VFI12_03350"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET7055469.1"
                     /translation="MIASLLVALLMLSPAVYLAIRASDAGTAAFDLLRRPRNIDIIRN
                     SVILSGLVGFSSALIGVPFAWLLERTDLPLRRLFSILSPLPLAIPSYVGALTYIEFLG
                     PRGLLQRFLARHTGIETIPSIYGFWGSFLALTLFTFPYVLLQVRATFTRLDPSFDDAA
                     RSLGQGPWRTFVRTTLPLLRPAIVAGSLLSALYAISDFGVVTLLRYDTFTRAIYTQYR
                     SSFNRSNAAVLALVLVLFAALLVSAEIALRGRAVYHRVGGGARRQPKRIALGRWTPVA
                     LGFCLIVLLLGLGIPLFVIAWWLIGRQGQVESTSRLLYAIWHSLELGAIVGLATLVVA
                     LPVALLSVRFRSRLANTIERICYLGYGLPGIVIALTFVFFGIRVATPLYQTLPLLIIA
                     LAIRFVPQALGAERASLLTVNPRVEEAARSLGRTAGSTFAHVTLPLARSGFVAGWALV
                     FVAVMKELPITLLLAPIEYRTLATEIWTASGSGAYSDAAIPALILVVIASLPTLLFRT
                     DQRDASTPSDEPAPRPPAANPPAT"
     gene            1932..3056
                     /locus_tag="VFI12_03355"
     CDS             1932..3056
                     /locus_tag="VFI12_03355"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7055470.1"
                     /translation="MSQHPDLLLPTRPPPDTALPMALVCRGVTKRFGNLEVVRDVDFD
                     VPAGSFTALLGPSGCGKTTMLRMIAGFERVDAGTIELNGRLISGPGRHEPPERRRVGM
                     VFQEGALFGHLDVAANVGFGLGRSDNRETVIAETLRMVGLTGYEHRMPHELSGGQQQR
                     VALARALAPRPEILLLDEPFSALDAELRVQVRVEVRSLLRSLGTTAVLVTHDQEEALS
                     LSDRVAVMMAGRIIQQDSPETLYHRPATRQIATFVGDAQFVDGTAAGRRVQTELGSLP
                     LTSAADGPVSVMIRPEQIRLVPPRIGEPANAIVEDRLFFGHDQLLRVRLQTGSTVQVR
                     LGAYGGIRPGHIVHVAVRGAVTAFPQAGGEQTRRRAGEQR"
     gene            3147..3605
                     /locus_tag="VFI12_03360"
     CDS             3147..3605
                     /locus_tag="VFI12_03360"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7055471.1"
                     /translation="MATIILICGLPGSGKTTLANQLARVRPALRLGEDDWMVQLFASP
                     DGHGEDQREAIKRVQWAIATNVALLGIDVVLDWGFWSRRERDDYRARAATLDIPTELR
                     FLDVPRDELIRRLAVRDADRPPATFAVAEADLNRWWDAFERPGADELNPP"
     gene            complement(3730..>3924)
                     /locus_tag="VFI12_03365"
     CDS             complement(3730..>3924)
                     /locus_tag="VFI12_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055472.1"
                     /translation="WVRNVFVAGEATIEYGGRDVAVDAPRLTNTAEAQRWLPWPVRIA
                     LRVMRVGDFLVVRCGKSAFY"
BASE COUNT          555 a         1469 c         1213 g          687 t
ORIGIN      
        1 gagcgggggc gaggggtgat ggttaccata ttctcgtaca cgcggggctg ctctctcagc
       61 ggtaatggcg gggctccgta cccgccttgg actccacggg cgaccagtgc gcggagcgcg
      121 gcccggccct cggtcagcct cggagcggcg ttctgcctga gcccggggtt tcaactccgg
      181 gtagtgcccg taccgtgccg acgatgcatc gggcaagtgc aggcgggtac ggagcccgcc
      241 acgacaggca actgactacc ggctactgat tactggctac tggcgcatgg ttgcaagcac
      301 gatcgaccga caaccgaaaa cggcccccgc cgtcccccgc ccacgcggga gctcggtcca
      361 ccgtgccccc ctgccgctgg tgatcgcgtc gctcctggtc gcgctgctca tgctctcccc
      421 ggcggtctac ctcgcgatcc gggcatcgga cgccggcacc gccgcattcg acctcctgcg
      481 ccgtccccgc aacatcgaca tcatccgcaa ctcggtcatc ctctccgggc tggtcggatt
      541 cagctcggcc ctgatcggcg tgccctttgc ctggctgctg gaacggaccg acctgcccct
      601 gcgccggctc ttctcgatcc tttcgccgtt gccactggcg atcccgtcct acgtcggcgc
      661 gctgacctac atcgagttcc tcggcccgcg cgggctgctc cagcgattcc tcgcccggca
      721 caccggaatc gagacgatcc cgagcattta cggattttgg ggatcgttcc tcgccctgac
      781 gctcttcacc ttcccgtatg tgctgcttca ggtccgcgcg accttcaccc gcctcgatcc
      841 ctccttcgac gacgccgccc gttcgctcgg gcaggggccg tggcgcacct tcgtccggac
      901 cactctgccg ctgctccggc cggccatcgt cgccggatcg ctgctctcgg ccctctacgc
      961 gatctccgat ttcggtgtcg tcactctgct tcggtacgac accttcaccc gcgccatcta
     1021 cacgcaatac cgctcctcgt tcaaccgcag caacgcagcc gtcctcgccc tggtcctcgt
     1081 cctcttcgcg gcgctgctcg tctcggccga aatcgcgctc cggggccgcg ctgtctacca
     1141 ccgggtcggc ggcggcgcgc gccggcaacc gaagcggatc gcgctcggcc gctggacgcc
     1201 ggtcgcgctc ggtttctgcc tgatcgtgct cctgctcggc ctcggcatcc cgctcttcgt
     1261 gatcgcctgg tggctgatcg gccggcaggg acaggtcgaa tcgaccagcc ggctgctcta
     1321 cgcgatctgg cactcgctcg agctgggggc gatcgtcggg ctggcgacac tcgttgtcgc
     1381 cctgccggtc gcgctgctct cggtccgctt tcgcagccgc cttgccaaca cgatcgagcg
     1441 aatctgctac ctcggatacg gcctgcccgg catcgtgatc gcgctgacct tcgtcttctt
     1501 cgggatccgg gtcgcgacgc cgctctacca gacgctgccg ctcctgatca tcgccctggc
     1561 gatccgtttc gtgccgcagg cccttggggc ggagcgggcc tcgctcctga ccgtcaatcc
     1621 ccgggtcgag gaggccgcgc gcagcctcgg ccgcaccgcc ggctcgacgt tcgcgcacgt
     1681 cacgttgccg ctcgcccgct ccggcttcgt cgccggctgg gcgctggtct tcgtcgccgt
     1741 gatgaaggag ttgccgatca cgcttctcct cgcgccgatc gagtaccgca ccctcgccac
     1801 cgagatctgg acggcatccg gctccggggc ctacagtgat gcggcgatcc cggcgctgat
     1861 cctggtcgtg atcgccagcc tgccgaccct gctcttccgc accgaccaac gagacgcgag
     1921 caccccgtcc gatgagccag caccccgacc tcctgctgcc aacccgcccg ccacctgaca
     1981 ccgccctgcc gatggcgctg gtctgccggg gcgtcaccaa gcgctttggc aacctcgaag
     2041 tcgtccgcga cgtcgatttc gatgtccccg ccggctcctt caccgccctg ctcggcccct
     2101 ccggctgcgg taagacgacg atgctgcgca tgatcgccgg cttcgagcgg gtcgacgccg
     2161 gcacgatcga gctcaacggc cggttgatca gcgggcccgg ccgccacgag ccgccggagc
     2221 gccgccgggt tgggatggtc ttccaggagg gagcgctctt cggccacctc gatgtcgccg
     2281 ccaacgtcgg gttcggcctc ggccgctcgg ataaccggga aaccgtcatc gcagagacgc
     2341 tgcggatggt cggcctgacc ggctacgagc accggatgcc gcatgagctg agcggcggcc
     2401 agcagcagcg tgtcgccctc gcccgggccc tcgcgccccg gcccgagatc ctgctgctcg
     2461 acgagccctt ctccgccctc gatgccgagc tccgtgttca ggtccgggtc gaggtccgct
     2521 ccctgctccg ctcgctcggc acgacggccg tcctcgtcac ccacgaccag gaggaggcgc
     2581 ttagcctctc agaccgcgtc gctgtgatga tggcggggcg gatcatccag caagactcgc
     2641 ccgaaacgct ctatcaccgc ccggccaccc gccagatcgc gaccttcgtc ggcgacgcgc
     2701 agtttgtcga tggaacggcc gctggccgcc gcgtccagac cgagcttggc agcctgcctc
     2761 tcaccagcgc tgccgatggg ccggtcagcg tcatgatccg gccggagcag attcgcctcg
     2821 tcccaccccg catcggggag ccggccaacg cgatcgtcga ggaccgcctc ttcttcggcc
     2881 acgaccagct cctgcgcgtc cggctgcaaa ccggcagcac cgtccaggtt cgcctcggcg
     2941 cctacggcgg catccgcccc ggccacatcg tccacgtcgc cgtccgcggc gcggttacgg
     3001 catttccgca agctggaggc gagcagacga gaaggcgagc aggcgagcag cggtaggagc
     3061 agacggcatc tgcccgttcc gtgctacgcg gcgaagcgga caatacggtt acgtgctggc
     3121 gagtggccgg tgcaatcgag aacagtatgg ccacgattat cttgatctgc ggtctgcccg
     3181 gttccggcaa gaccacgctg gccaaccaac ttgcgcgtgt tcgccccgcc ctccggctcg
     3241 gcgaagacga ctggatggtg cagctcttcg cttcgcctga tggccacggg gaagatcagc
     3301 gcgaggcaat caagcgggtg caatgggcga tcgcgacgaa tgttgccttg ctcgggatcg
     3361 atgtggtgct cgactgggga ttctggtcgc gccgggaacg cgatgactac cgcgcccggg
     3421 cggcaacact cgacatccct accgagctgc ggttcctcga tgtgccgcgc gacgaactga
     3481 tccgccgcct cgccgtgcgc gatgccgatc gtccgccggc tacgtttgca gtggccgagg
     3541 ccgatttgaa ccgctggtgg gacgcattcg agcgaccggg tgcggacgaa ttgaatccgc
     3601 cgtgaagtcc tacagcagta tcggatgaag cgccagttca gaactagtag cccggggcgg
     3661 cctcgagctg ctctcaggta cagaccgacc gggcgggccg cccggggcta catcaccgaa
     3721 tagtcgcgtt taatagaacg cgctctttcc gcaccgcacc accaggaaat cgccgactcg
     3781 catcactcgc agcgcgatcc ggaccggcca aggcagccac cgctgtgcct cggccgtgtt
     3841 cgtcagccgc ggcgcatcta ctgccacatc ccggcccccg tactcgatcg tcgcctcacc
     3901 ggccacaaac acgttccgca ccca
//
LOCUS       DASSGX010000190         4114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1232879, whole genome shotgun sequence.
ACCESSION   DASSGX010000190 DASSGX010000000
VERSION     DASSGX010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232879"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1577
                     /locus_tag="VFI12_03370"
     CDS             <1..1577
                     /locus_tag="VFI12_03370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HET7055473.1"
                     /translation="MRGLTVCLLEMNDLASGTSAWSSRLVHGGLRYLEHYEFGLVRES
                     LRERERLLHNAPHLVSPLPMIVPIHEGAKRGPLLIRAGMVLYDVLSYDKSMPLHRMLS
                     AEKVIERVPALDPDGLRAAAVYYDAQVTFAERLVIENVLSAVEHGAQIVTQATVTGLC
                     SEGAVVRAVEVHDDITGSDHRISGRAIVNVAGPWVDQVLAEAPEGRIRDQFIGGTKGS
                     HIIVDRFDGAPADAIYYESRSDRRPILVIPFNGMIMLGSTDIRYDGDLDRVEASDAEI
                     EYLLNEANALFQGVNLTADDVRYSYAGIRPLPYTPNGSTASITRRHIIKSHAPRLRGL
                     WSIIGGKLTTHRSLAEELVDAVVTQLGRDATCQTAETQLPGAAGVAFDTYRASLVRPA
                     SEAGVSERSAARLAEVYGTRAEQILAIVRQKPDLATVVDPYSGAIAAEAVFAARSEMA
                     LSLADILLRRTMIAYGPHAGIGPDEHLLEAAGKEIGWTKPQQKKELAAFREWITRYRP
                     LRREEGAEARGREGAK"
     gene            1941..2324
                     /locus_tag="VFI12_03375"
     CDS             1941..2324
                     /locus_tag="VFI12_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055474.1"
                     /translation="MGRALLTLAWIILLLLLVDSAMHDPDVQPVVDRQITRMERSLGR
                     ITGWLRAQRSSVSSSLAEANPRTASLDHSFFVGPVETWYWSTTLGVAATLAGLAKSTW
                     RELCRLPAACAVSSRATDDRAGRRS"
     gene            2321..3283
                     /locus_tag="VFI12_03380"
     CDS             2321..3283
                     /locus_tag="VFI12_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055475.1"
                     /translation="MIALAAPLGQAANAESSTDGWRLIGLIGITLATIAILALIFRPR
                     NGAEPSPDGGEPAPIDDFETPGLLRFPGTPATPQTDAELAAERLRQIVGRGAPDERHR
                     PAGAPPARRGEPADESASEDTAMETGEESRIPGMGEAGIDAEALEEQLRRLFGPHGEP
                     GKIVPFTPRKPQPPGTPEGQPAGFEEPRRTRAESRITRFPVDTATRDGITATIQELLF
                     CANVGELLHGFALYTDRYLFQFMDESGMNENEFRAAYSDIPAKDPADWTRIDSITDFA
                     RLDDGRVTVRVRYIDGSAIDGTEQFTMRYDAHLNRWLIDNIQAS"
     gene            3364..>4114
                     /locus_tag="VFI12_03385"
     CDS             3364..>4114
                     /locus_tag="VFI12_03385"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET7055476.1"
                     /translation="MYTTLVGCPISEIDTPALVIDLDALDHNIATMAADITARGAQWR
                     PHSKANKTPAIVHKELQAGAIGVTCAKVSEAEVMAANGIRDILIANQVVGPTKTRRLA
                     WLAREADVIVAVDNPDNVRELDGAAGHAGSKPRILIEVNTGMERCGALPGDPTVELAK
                     LVDSMPNLRFAGVMAWEGHSMGIKDPAAREAEIKRACGALVDTANACRAAGLDVQIVS
                     AGGTGTYLISAGVEGITEVQAGGGIFGDSTYR"
BASE COUNT          733 a         1360 c         1355 g          666 t
ORIGIN      
        1 cgatgcgcgg actgaccgtc tgcctgctcg agatgaatga cctcgcgagc gggacgtcgg
       61 cctggtccag ccggctggtt cacggcgggc tgcgctacct cgagcattac gagttcggac
      121 tcgtgcgcga atcgctccgg gagcgcgagc gcctgcttca caatgcgccg cacctggttt
      181 ctcccctgcc gatgatcgtt ccgatccacg agggcgccaa gcggggaccg ctcctgatcc
      241 gggccggcat ggtcctctac gatgtgctct cgtatgacaa gtcgatgccg ctccatcgaa
      301 tgctctcggc ggagaaggtg atcgagcgag tgccggcgct ggatccggac ggattgcgcg
      361 cggccgctgt ctactacgac gcccaggtta cctttgccga gcggctggtc atcgaaaacg
      421 tgctctcggc ggtcgaacat ggggcacaga tcgtcacgca agcgaccgtg accggactct
      481 gctcggaagg ggcggtcgtc cgcgcggtcg aggtccacga cgacatcacc ggcagcgatc
      541 accggatttc cgggcgggcg atcgtcaacg tcgctggacc atgggtcgac caggtgttgg
      601 ccgaggcgcc ggagggacgg attcgcgacc agttcatcgg cggcaccaag ggcagccaca
      661 tcatcgtcga tcgcttcgac ggcgcgccgg ccgacgcgat ctactacgag tcgcgctccg
      721 accgccgccc gatcctcgtc atcccgttca acggcatgat catgctcggc agcacggata
      781 tccgctacga tggcgacctc gatcgggtcg aggcgtcgga cgcggagatc gaatacctgc
      841 tcaacgaggc caacgcgctt ttccagggcg tgaacctgac ggcggacgac gtacgctatt
      901 cctacgccgg tatccggcca ctcccgtaca cgccgaacgg cagtacggca tcaattaccc
      961 gccgccacat catcaagagc cacgcgcccc ggctacgcgg tctctggtcg atcatcggcg
     1021 gcaagctgac gacgcaccgc agcctggccg aggagctggt cgatgcggtg gtgactcagc
     1081 tcggccggga tgccacgtgc cagaccgccg agacgcaact cccgggcgcg gccggtgttg
     1141 ccttcgacac ataccgggcg agtttggtcc ggccggcgtc cgaggccggc gtatccgagc
     1201 ggtctgccgc tcgcctggcg gaggtgtacg ggacccgggc agagcaaatc ctggcgatcg
     1261 tccgccagaa gccggacctg gcgaccgtgg tcgacccgta ttcgggcgcg atcgcggccg
     1321 aggcggtttt cgccgcgcga tccgagatgg cgttgtcgct cgccgacatc ctcctgcggc
     1381 ggacgatgat cgcctacgga ccgcacgccg gcatcggccc ggacgagcac ctcctcgagg
     1441 ccgccggcaa ggagatcggc tggaccaagc cgcagcaaaa gaaggagctg gctgccttcc
     1501 gggagtggat cacgcggtat cggccactga ggcgagaaga aggcgcggag gcgcgagggc
     1561 gcgaaggcgc gaagtaggga tccgcgggga cagatccgcc cccttgcgaa gcgacgccgg
     1621 acggccgtct gtcaaactat cttggcgacg cctcttcatc cggtgcgtcg ctgtagtcgc
     1681 ccgttccggt cccactcggc atcaatcatt atccccagac actcttgggc gctcgatcct
     1741 gtcgcgtgac ggtcgcctat tcctgctcat cccgatggca tgcggtacct tggtatcgcg
     1801 tggaatagga ccgtctcttg cctgccgcga ttgattcgac gtagcggtga aatatacctt
     1861 tcgggctagg aaactagtac tgcctcgcgt ataggccgat tgggtcttat tcgcgggtca
     1921 ggtatcccga aaggcccccc gtgggacgcg ccctgctcac gctcgcctgg atcattctgc
     1981 tgttgctgct cgtcgacagc gcgatgcacg atcccgacgt ccagcccgtc gtggatcgcc
     2041 aaatcacgcg catggagcgc tccctgggcc ggatcaccgg ctggctcaga gcccagcggt
     2101 ccagcgtcag cagcagcctg gcggaagcca acccgcgcac tgcgtccctc gaccactcat
     2161 tttttgtcgg tcctgtcgag acatggtact ggagtaccac gctgggcgtg gcggcgaccc
     2221 ttgccggctt ggcaaagtca acatggagag aactttgccg attaccggcc gcctgcgcgg
     2281 tttccagtcg agccactgac gatcgcgcgg gtcgtcgatc atgatcgcgc tcgcggcgcc
     2341 actcgggcag gccgccaatg ccgaatcgtc cacggacgga tggcggctga tcggcctgat
     2401 cgggatcacg ctggcgacga tcgcgatcct ggccctgatc ttccggccgc gaaacggcgc
     2461 cgaaccgagt ccggacggtg gcgagccggc gccgatcgac gacttcgaga cgcccggatt
     2521 gcttcgcttc ccgggaacgc cggccacccc gcaaaccgac gcggagttgg ccgcggagcg
     2581 acttcggcag atcgtcggcc ggggcgcccc ggacgaacga catcgacccg cgggcgctcc
     2641 acccgcgcga cgcggcgaac ctgccgacga gagtgcgagt gaggacaccg ccatggagac
     2701 aggcgaagag agtaggatcc cgggaatggg cgaggccgga atcgacgcgg aagcgctcga
     2761 ggaacagctc cggcgattgt tcggtccgca cggggagccg ggaaagatcg tgccgttcac
     2821 gccacgcaag ccacagccgc cggggacgcc ggagggacag ccggctggtt tcgaagagcc
     2881 gcggcgtacc cgggccgaat cacggatcac ccgctttccg gtcgacaccg cgacgcggga
     2941 cggcatcacg gcgacgatcc aggagctgct cttctgtgcc aatgtcggcg agctcctgca
     3001 cggcttcgcg ctctacaccg accgctacct cttccagttc atggacgaat caggcatgaa
     3061 cgagaacgag ttccgagcgg cctacagcga catcccggcc aaggatccag ccgactggac
     3121 caggatcgac tcgatcacgg atttcgcgcg gcttgacgac ggccgggtaa cggtccgggt
     3181 ccggtacatc gacggctccg ccatcgacgg caccgagcaa ttcacgatgc gctacgacgc
     3241 gcatcttaat cgctggttga tcgacaacat tcaggcgagc taaattcgaa ccgggtatga
     3301 tggcgaagcc tcgggagcgc accggggctt ctttgttgca atgaaacacg aggcatccgg
     3361 aagatgtaca ccacgctcgt aggctgtccc atcagcgaga tcgatacgcc ggcgctcgtc
     3421 atcgacctgg atgccctcga ccacaacatc gcgaccatgg cggccgatat cacggcccgc
     3481 ggtgcgcagt ggcgcccgca cagcaaggcc aacaagacgc cggccatcgt ccacaaggag
     3541 cttcaagccg gcgcgatcgg tgtgacctgc gcgaaggtca gcgaggccga ggtcatggcg
     3601 gcgaacggca tccgcgacat cctgatcgcg aatcaggtcg tcgggccgac caagactcgc
     3661 cgcctggcct ggctggcccg cgaggccgac gtgatcgtgg cggtcgacaa cccggacaac
     3721 gtccgcgaac tggacggcgc ggccggtcac gccgggagca agccccgcat cctgatcgag
     3781 gtcaacaccg gcatggagcg ctgcggcgcc ctgcccggcg acccgactgt cgagctggcg
     3841 aagctggtcg attcaatgcc gaacctgcgt tttgccggtg tgatggcctg ggaaggtcac
     3901 tcgatgggca tcaaggaccc tgctgcccgc gaggctgaga tcaagcgcgc ctgtggtgcg
     3961 ttggtcgata cggccaatgc ttgccgggcg gccgggctcg acgtccagat cgtctccgcc
     4021 ggcggcaccg gaacctacct gatctcagcc ggagtcgagg gcattactga agtccaggcg
     4081 ggtggcggca ttttcggcga tagcacctac cgtg
//
LOCUS       DASSGX010000191         2675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_688926, whole genome shotgun sequence.
ACCESSION   DASSGX010000191 DASSGX010000000
VERSION     DASSGX010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688926"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VFI12_03390"
     CDS             <1..339
                     /locus_tag="VFI12_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055477.1"
                     /translation="AQMVAGFGLVEQTVIDQHFRQRDRTGRLLSLIAGSPGLLGVGVD
                     EDTAAIFGADGTIDVVGRHSVTIIDGSHMFTDYPEIKGFGEITVSGAVIHSLIPGRRF
                     DSGSRRMMPL"
     gene            370..>2675
                     /gene="cphA"
                     /locus_tag="VFI12_03395"
     CDS             370..>2675
                     /gene="cphA"
                     /locus_tag="VFI12_03395"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: protein motif:HMM:NF010623.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HET7055478.1"
                     /translation="MIEVRKTTVYRGPNVWARMPVILMVVDIGELEDRPTNKIPGFYE
                     RLTEYMPSLYDHGCSLGRPGGFLQRMREGTWMGHVLEHVALEFQNLSGAEVARGKTRS
                     TGERGVYNVVFEHVQEDVGLVAGRLAVRLLNHLIYNSEPEFNFQAELEENVIRVAERL
                     AYGPSTGSIVSEAERRNIPVLRLDPKRSLVQLGHGIHQQRAWATVTSRTSNIAVDIAA
                     NKELTNRLLQEVGIPVPRSQVVRTVDDAVNVANRIGYPVVIKPLDGNHGRGVCINLAD
                     DDEIREFYPVAEGESRNGIVQVESYITGRDYRILVVNGQVVAVAERVPAHVIGDGEHS
                     VEQLIEITNSDPRRGVGHEKILTRISIDNQTMETLERQGIALIDVPALDQVVKLKQTG
                     NMSTGGTSIDRTDDIHPDNIEIARQAAMVVGLDIAGIDFITSDIAESVRQSGGAIVEV
                     NAGPGFRMHTNPTEGHPRHVGRAVVDMLFPPGAKSRVPIVAVTGTNGKTTTVRMISHI
                     MKTAGRRVGMTTTDGIYIDGTQILAGDMSGPTSAQMVLKNPTIDYAVLETARGGILRS
                     GLGFDRCDIAVVTNVTSDHLGLGGIESMEDLSRVKAVVPQAVFRDGVSVLNADNEWTV
                     DMARTARGEIIFFSMNEDSPVLREHLRQRGRAVVLRPTRHGDMITIIEHRRETSLILA
                     SEIPATFGGRLRVNIANAMAAASAAVGGDIQFEHIRQGLRSFSSSYFQTPGRFNFLDV
                     DGRRVVMDYCHNVAGLESMADFVQRLGAP"
BASE COUNT          502 a          842 c          884 g          447 t
ORIGIN      
        1 gcgcagatgg tggcgggatt cggcctcgtc gagcagacgg tcattgacca gcacttccgg
       61 cagcgggacc gcaccggccg gctcctgtcg ctgatcgcgg gcagtccggg cctgctgggc
      121 gtcggagtcg acgaggacac cgcggccatc ttcggagccg atgggactat tgacgtggtc
      181 ggccggcatt ccgttacgat tatcgacgga tcacatatgt ttaccgatta cccggaaatc
      241 aaggggttcg gcgaaatcac cgtcagcgga gcggtgatcc attcgctcat tcccggccgc
      301 cgattcgatt ccggtagccg acgaatgatg ccgttgtagc aatcacgcga cgtccagggg
      361 cggggaccaa tgatcgaagt tcggaagacc actgtttatc ggggtccaaa cgtctgggcg
      421 cgcatgccgg tcatcctgat ggtcgtcgac atcggcgaac ttgaggaccg gccgaccaac
      481 aagatccctg gcttctatga gcggctgacc gagtacatgc cgtcgctgta cgaccacggt
      541 tgctcactcg gccgacccgg tggcttcctg caacggatgc gcgaaggcac gtggatgggc
      601 catgtcctcg agcatgtcgc gctcgagttc cagaacttgt ccggcgcgga agtcgcccgg
      661 ggcaagacgc gcagcaccgg cgagcgcggc gtctacaacg tcgtgttcga gcatgtgcag
      721 gaggatgtcg gacttgtagc cgggcggctg gcagtccgac tgctcaacca cttgatctac
      781 aactccgagc cggaattcaa tttccaggct gagcttgagg aaaacgtgat tcgcgtcgcg
      841 gagcgtcttg cctacggccc gtcgacgggg tcgatcgtct ccgaggccga gcgccggaac
      901 atcccggtcc tgcgactcga tcccaagcgc tcgctcgtcc agctcgggca cggcatccat
      961 cagcaacgag cctgggccac ggtgacgtcg cggacgtcga acatcgcagt cgatatcgcc
     1021 gcgaacaagg agctgaccaa ccgcctgctg caggaggttg gcatcccggt gccgcgcagc
     1081 caggttgtcc gcaccgtcga tgacgcggtg aacgtcgcca accggatcgg ctacccggtc
     1141 gtcatcaaac cgctcgatgg caaccacggc cgtggagtct gcatcaatct ggcggacgac
     1201 gatgagattc gcgagtttta cccggtcgcg gagggcgaga gccggaatgg gatcgtccag
     1261 gtcgagagct acatcaccgg ccgcgattac cggatcctcg tggtcaacgg ccaggtcgtg
     1321 gcggtggcgg agcgcgtgcc ggcccatgtc atcggggatg gcgagcattc agtcgagcaa
     1381 ctgatcgaaa tcacgaactc cgatccccgg cgcggtgtcg ggcacgagaa aatcctgacc
     1441 cgaatctcga tcgacaacca gacgatggag acgctcgaac gacagggaat cgctctgatc
     1501 gatgtgccgg cgctcgacca ggtcgtgaag ctcaagcaga cgggcaacat gagcaccggc
     1561 ggcacgtcga tcgaccgcac cgacgacatc cacccagaca acatcgagat tgcgcgccag
     1621 gcggcaatgg tcgtcggcct ggatatcgcc ggcatcgact tcatcacgtc cgacatcgcc
     1681 gagtcggtcc ggcagagcgg tggcgcgatc gtcgaggtca acgccgggcc cggcttccgg
     1741 atgcatacga acccgaccga gggtcatccg cgccacgtcg gccgcgcggt cgtcgacatg
     1801 ctgttcccgc caggcgcgaa atcgcgcgtg ccgatcgtcg cggtgacggg cacgaacggc
     1861 aagacgacga cggtccgcat gatttcgcac atcatgaaga cggcgggacg gcgcgtcggc
     1921 atgaccacga ccgatggcat ctatatcgac ggcacgcaga tcctggccgg cgatatgagc
     1981 ggcccgacca gcgcgcagat ggtcctcaag aacccgacga tcgactatgc ggtgctcgaa
     2041 acggcgcgcg gcggcatcct ccgctccggg ctcggcttcg accgctgcga catcgcggtg
     2101 gtgacaaacg tcacgtccga tcacctcggc ctcggcggga tcgaatcgat ggaggacctg
     2161 tcgcgggtca aggcggtcgt gccgcaggcg gtcttccggg acggcgtctc ggtgctgaac
     2221 gccgataacg agtggactgt cgacatggcg cggacggccc gtggcgagat catcttcttc
     2281 agcatgaacg aggactcacc cgtcctgcgc gagcatctgc ggcaacgggg ccgggccgtc
     2341 gtcctgcgac cgacgcgaca cggcgacatg atcacgatca tcgagcaccg ccgcgagacg
     2401 agcctgattc tggcgagcga gattccggcg acgttcggcg gccggttgcg ggtcaatatc
     2461 gccaatgcga tggcggctgc ctcagcggct gtcggtggcg acatccagtt cgagcacatc
     2521 cggcaggggt tgcgcagctt ctcgtcgagc tatttccaga cgccgggccg gttcaacttc
     2581 ctggacgtgg acggccggcg ggtggtcatg gactactgcc acaacgtggc cggcctggaa
     2641 tcgatggccg acttcgtaca gcggctcggc gcgcc
//
LOCUS       DASSGX010000192         2340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_36840, whole genome shotgun sequence.
ACCESSION   DASSGX010000192 DASSGX010000000
VERSION     DASSGX010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36840"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..1130)
                     /locus_tag="VFI12_03400"
     CDS             complement(18..1130)
                     /locus_tag="VFI12_03400"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HET7055479.1"
                     /translation="MPAAHAGHARMMNNAGIVQLRLTQHGLSAPGALSPTEVVRRQGA
                     MQSQEFGPAKWSIGQRIAGSTEPEVSRSFDDGAFLRTHVLRATWHFVAAEDIRWLLEL
                     TGPRIHQGNAGRYRELELDAATRAKAGRAIVAAVEGGRHRTRTDLADHLTCAGISTEG
                     QRMPYLLMHAELEGLICSGPVRGKQQTYALLSERAPQATSRSRDEALAELVRRYFTSH
                     GPATIADFTRWCSLTVADTKRGIELLGNEMQSFTYDGRTWWHAELPAEVATEPSPTAH
                     LLQAYDEYVNGYGESRDVVDPFIHATFRGNGYMPVVIIDGQVAGSWQRTLRAKEVQIE
                     IHPFRPLTPAEHEAINAATGAHSRFVGLVPRVSIPL"
     gene            complement(1090..1464)
                     /locus_tag="VFI12_03405"
     CDS             complement(1090..1464)
                     /locus_tag="VFI12_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055480.1"
                     /translation="MHANVTIWLITEIARKSDSIQAFLQSWRPGGYREFFRIAAPTII
                     PVLRAHGLIFAFAVRTQADRLTIVTIYEDEAGAEAGWYAITGNLHEVMEGHLELLERT
                     SGPVEDLAELACGPCWPRPDDE"
     gene            1766..>2340
                     /locus_tag="VFI12_03410"
     CDS             1766..>2340
                     /locus_tag="VFI12_03410"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7055481.1"
                     /translation="MESPSYIVAELPSHARVVVVGAGIVGVSAAYELTRRGISDVVVI
                     DRGDLFRTGGSTSHAPGGVFQNNTSRTVSKLAQWSVDTFLDTNSDSETPVYWPTGSLE
                     VATTEARWQDLHRKYGYARSWGLDANLLSPTEVKQRLALIDESTILGAIHIARDGDLL
                     AVPFVDRLAARAGNQGARFIGRTKVTGFEID"
BASE COUNT          377 a          759 c          753 g          451 t
ORIGIN      
        1 gccgcagagc gtgttggcta gaggggtatc gacactcgcg gaacaaggcc aacgaatcgg
       61 ctgtgggcgc cggtggcggc gttgatagcc tcgtgctcgg cgggggtcag ggggcggaag
      121 gggtggatct cgatctgcac ctctttggcg cggagggtgc gctgccagct ccccgcaacc
      181 tggccatcga tgatgacgac cggcatgtac ccgttgcccc gaaaggtggc gtggatgaac
      241 ggatcgacga cgtcgcggct ctcgccgtag ccgttaacgt actcgtcgta ggcctggagc
      301 agatgcgcgg tcggcgaggg ctcggttgcg acctcggccg gcaattcggc gtgccaccag
      361 gtccggccgt cgtacgtgaa gctttgcatc tcattaccga gcaattcgat cccgcgcttc
      421 gtgtcggcga cggtcaggct gcaccagcgg gtgaaatcgg cgatcgtcgc cggaccgtgg
      481 ctcgtgaaat agcggcggac caactctgcc aatgcctcat ctcgcgagcg cgacgtcgcc
      541 tggggcgctc gttcgctgag gagcgcgtac gtctgctgct tgccccggac cggcccgctg
      601 cagattagcc cctcgagctc ggcgtgcatc aacaggtagg gcatccgctg cccttcggtg
      661 gaaatgccgg cgcaggtgag gtgatctgcc agatcggtcc gggtccggtg tcgcccgccc
      721 tcgaccgcgg cgacgatcgc ccggccggct ttggcacgag tcgccgcgtc caattccagt
      781 tcgcgatacc ggccggcgtt gccctggtga atgcgcggac cggttagctc cagcagccag
      841 cggatgtcct cggccgcgac gaagtgccat gtcgcccgca gaacgtgtgt ccgcaggaac
      901 gctccgtcgt cgaatgacct gctgacctcc ggctcggtcg atcccgcgat ccgctggccg
      961 atgctccact tcgccggacc gaactcctgc gactgcatcg ccccctgccg gcggacgacc
     1021 tcggtcggag acagcgcgcc gggcgcggaa agaccgtgct gagtgaggcg aagttggacg
     1081 atcccggcgt tattcatcat ccgggcgtgg ccagcatggg ccgcaggcaa gctcggcgag
     1141 gtcctcgacg ggtcccgacg tgcgctcgag gagttccagg tggccctcca tgacctcgtg
     1201 caggttgccg gtgattgcgt accaacctgc ttccgccccg gcctcgtcct cataaatggt
     1261 gacgatcgtg agccggtcag cctgggtccg aaccgcgaac gcgaagatca ggccgtgggc
     1321 tcgaagcacc ggaatgatcg tcggcgcggc gatccggaag aactcccggt atccgccggg
     1381 ccgccaggac tgcaggaacg cctggatgct gtcggatttc cgcgcgattt cagtgatcaa
     1441 ccagatcgtg acgtttgcgt gcacggccct atccgctaac ccaacccata tccgacacgt
     1501 gtcgtctctc cccagagaac tgcatgataa tacaaacatt tgtttcaagt tctgccgaag
     1561 aagtccctga ttccgccata ccttccgccc ggcatgccca aatcacgcaa tgcgatgttg
     1621 tttcgggtct ctggtctctg gtctctggtt tttcgttttc cgttttcggt tttccgttga
     1681 ctcacgactc atgactcacg tcgcacgact gtgtggggct gctcgccttc tcgcctgctc
     1741 gcctgctcgc ctatagaatg cgctcatgga atcgccgagc tatatcgtcg ccgaattgcc
     1801 gtcccatgct cgcgtcgtgg tcgttggggc ggggatcgtc ggagtttccg ccgcgtacga
     1861 gctgacgagg cggggcatct ctgatgttgt cgtgatcgac cggggtgacc tgttccggac
     1921 cggtggctcg acgtcgcatg cgccgggcgg cgttttccag aacaacacct cgcgcaccgt
     1981 ctcgaaactg gcccaatggt cggtcgatac cttcctcgac acaaacagcg acagcgagac
     2041 tcccgtctac tggcccaccg gcagccttga ggtcgcgacc accgaggctc gctggcagga
     2101 cctgcaccgc aagtacggtt acgcccgctc gtggggcctc gacgcgaacc tgctctcgcc
     2161 gaccgaggtc aagcagcggc tcgcattgat cgacgaatcg acgattctcg gcgcgatcca
     2221 cattgcccgg gacggcgacc tgctggcggt tcccttcgtt gaccggctcg cggcccgggc
     2281 cggtaaccag ggagcgcgtt tcatcgggcg gacgaaggtg accggcttcg agatcgacga
//
LOCUS       DASSGX010000193         2401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1127335, whole genome shotgun sequence.
ACCESSION   DASSGX010000193 DASSGX010000000
VERSION     DASSGX010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127335"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..1063)
                     /gene="iolB"
                     /locus_tag="VFI12_03415"
     CDS             complement(170..1063)
                     /gene="iolB"
                     /locus_tag="VFI12_03415"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF016826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HET7055482.1"
                     /translation="MIDLLLHSTTPSAQDVSTLVTVGPEEAQWDYCSLTVLRMKPGAT
                     WQGSTSRDEIALVPLSGNCTVTSGDQSWSIGGRASLFDGPTSSLYLPINTEYTVTAKG
                     TVELAICGARAEKSFPPRFISADEVLIEIRGAGNAARQINHIIKPDFAADRLLVVEVY
                     TPSGNWSSFPPHKHDVSRMPAEADLEEIYYYRIDPADGFALQRLYTEDGRIDQAWTIK
                     DGDLLLVPEGYHAFAVAHGYTGYYLNILAGAEPIRTMQPADDPSYAWVRGTWNDEMNE
                     GARSFRDIDQRVNRGAGLRRK"
     gene            complement(1060..2088)
                     /gene="iolG"
                     /locus_tag="VFI12_03420"
     CDS             complement(1060..2088)
                     /gene="iolG"
                     /locus_tag="VFI12_03420"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR04380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HET7055483.1"
                     /translation="MTKRVRVAVIGAGRIGKRHARTLATQTPNAELVVIADAIESAAR
                     SLAEELRIARWTTDTDSVITDPNIDAVVIASSTNTHAPLTIAAAEAGKEVFCEKPIAL
                     DLEMTDKAIAAARNAGVQLQVGFNRRFDKGYRSAKADLDAGRIGKIESIRDTMRDPAP
                     PPRSYVATSGGLFRDMSIHDFDCVRWLAGSEPVELTAMAACLVDPMFAELGDVDTAIV
                     TIRFESGVLAVIDNSRRSSFGYDVRTEIFGSDGALFVGYHRDTPVTRFTSAGVMTDHA
                     YFFLERFDQAFVDEIRAFVEAVATRKPVSVTGEDGRAALALAYAAEASVKEKCPVQVA
                     RFTKGIPA"
     gene            complement(2085..>2401)
                     /locus_tag="VFI12_03425"
     CDS             complement(2085..>2401)
                     /locus_tag="VFI12_03425"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HET7055484.1"
                     /translation="LSEQEISAAVLHVPTLKPLDVDAIVAVAKRTNLILTTEDHSIIG
                     GLGGAIAECLGEHRPTRMSRIGIRDVFGESAPNDALLEKHGLTAAKVAEAAIALMEST
                     RR"
BASE COUNT          363 a          768 c          833 g          437 t
ORIGIN      
        1 cggtctcctc gagcgcctgg ccgaggccgg tcacgttgcc gtgaccgaag atgccccaca
       61 tcccggcgaa gaaaggctgg gtgacgccgt cccgctcgac ctgctgcttc gcgaggaaac
      121 gaaccaatgc ctgggccatc gtggcccgta ccgtcgtcat cgtcggctcc tatttccggc
      181 gcagacccgc gccgcgattg acgcgctggt cgatgtcccg gaaagaccgc gcgccctcgt
      241 tcatctcgtc gttccaggtt ccgcgcaccc acgcgtagga cgggtcgtcg gccggctgca
      301 tggttcggat cggttccgcg ccggcgagga tgttcaggta gtagccggtg tacccgtggg
      361 cgacggcgaa ggcgtggtag ccctcgggga cgagcagcag gtcgccgtcc ttgatcgtcc
      421 aggcctggtc gattcggccg tcctcagtgt agagccgctg gagggcgaag ccgtccgccg
      481 ggtcgatccg gtagtagtag atctcctcga ggtcggcctc ggccggcatc cggctgacgt
      541 cgtgcttgtg gggcgggaag ctggaccagt tgccgctggg cgtgtagacc tcgacaacca
      601 gcagtcggtc cgccgcgaag tcgggcttga tgatgtggtt gatctggcgg gcggcattcc
      661 ccgcgccccg gatttcgatc aggacctcgt cagcgctgat gaaccgcggc gggaacgatt
      721 tctcggcccg cgccccgcag attgccaact cgaccgtgcc cttggcggtg accgtgtact
      781 ccgtattgat cggcaggtac agggacgagg tcggcccgtc gaaaaggctg gctcgtccgc
      841 cgatgctcca cgactgatcg ccgcttgtga cggtgcagtt accggagagg gggaccagcg
      901 cgatctcgtc ccggctggtg ctgccctgcc aggtcgcgcc cggtttcatc cggagcacgg
      961 tcaggctgca gtagtcccac tgggcctcct ctggtccgac cgtgaccagc gtgctgacgt
     1021 cttgggcgga tggcgtcgtt gaatgcaaca acaaatcgat catgcgggga ttcctttcgt
     1081 gaaccgggcg acctggaccg gacatttctc cttgaccgag gcctcggcgg cgtaggcgag
     1141 ggcgagcgcg gcccggccgt cctcgccggt gacactgacc ggcttccggg tcgcgacggc
     1201 ctcaacgaag gcgcggatct catccacaaa cgcctggtcg aaccgctcga ggaagaaata
     1261 ggcgtggtcg gtcatgactc cggccgaggt gaagcgggtg accggcgtat cgcggtggta
     1321 accaacgaag agggcgccgt ccgagccgaa gatctcagtc cgcacgtcgt acccgaagct
     1381 ggagcggcgg ctgttgtcga tcacggccag gacgccgctc tcgaaccgga tcgtgacgat
     1441 cgccgtgtcg acatcaccca actcggcgaa catcgggtcg accaggcagg cggccatcgc
     1501 ggtcagctcg accggctcgc tcccggccag ccagcggacg caatcgaagt cgtgaatgga
     1561 catatcccgg aagagaccgc ccgaggtggc gacatacgag cgcggtggtg gggcgggatc
     1621 gcgcatggtg tcgcggatcg attcgatctt gccgatccgg ccggcgtcga ggtccgcctt
     1681 ggcgctccgg taacccttgt cgaagcggcg gttgaagccg acctggagct ggacacccgc
     1741 gttccgggcg gccgcgatcg ccttgtcggt catctcgagg tcgagcgcga tcggtttctc
     1801 gcagaagacc tccttgccgg cctcggcagc ggcgatggtt agcggcgcgt gggtgttagt
     1861 ggaggaggcg atcacgaccg cgtcgatgtt cgggtcggtg atgaccgagt cggtgtcggt
     1921 tgtccaccgt gcgatgcgta gctcctcggc caggctccgg gcggcggatt cgatcgcatc
     1981 ggcgatcacg accagctcgg cattcggcgt ttgggtggca agggtgcggg cgtgccgctt
     2041 gccgatccgg cccgcgccga tcacggcgac tctgacgcgc ttggtcatct cctggtgctc
     2101 tccatcagtg cgattgctgc ttccgcgacc ttcgcggccg tcaatccgtg cttctcgagc
     2161 agagcgtcgt tcggggctga ttcgccgaag acgtcccgga tgccgatgcg gctcatccgg
     2221 gtcgggcgat gctctccgag gcattcggcg atcgcgccgc cgagaccgcc gatgatcgaa
     2281 tggtcctcgg tggtcaggat caggttcgtc cgcttcgcca ccgccacgat cgcgtcgacg
     2341 tccagcggct tgagagtcgg gacgtgcagc acggccgcgc tgatctcctg ctcggacagc
     2401 a
//
LOCUS       DASSGX010000194         2289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_364670, whole genome shotgun sequence.
ACCESSION   DASSGX010000194 DASSGX010000000
VERSION     DASSGX010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364670"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..1181
                     /locus_tag="VFI12_03430"
     CDS             27..1181
                     /locus_tag="VFI12_03430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET7055485.1"
                     /translation="MVQQTLSIAHVTATFPPYFGGTGTVCFHNARVLASRGHRVSVYT
                     AEWDGQSDDPPGVEVHRLRPVVHVGNAPVMPQLLRMPRNAIVHLHFPFYAGGELVSLS
                     RRPYVVTYHQDVEIDGLLGRLTSAHDATIGRQVLRRARRLCPTSLDYFQSSRFAGLIP
                     AMANRISPIPNGVDTSVYRPGPPDPALRARYSLPESMPIVLFVGSMDRAHHFKGIPVL
                     LQALATTGDVAALLIGDGDLRRQYEQQAEQLGVADRVRFAGRVPAEDLPAAYRLAEVL
                     ILPSVTRGEAFGVVLLEAMASGRPVIASDLPGVRSVVSHGVDGFLIPPSDAAALANQI
                     SELLALDPAVRAEMGAAGRRKVEAHYDWERIGDQLEALYRGVLQETRRVA"
     gene            1178..1729
                     /locus_tag="VFI12_03435"
     CDS             1178..1729
                     /locus_tag="VFI12_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055486.1"
                     /translation="MSELIRLPFELRGALDYVAFRTGGVRGETGGRVVGDPILVNELG
                     RYAPIAPAPETVIVLLGQSEEIAEAVAQLSVTDAWIVTAGPLDRLMRPLRTRTTGEIR
                     PSAAVPAEQWRALGFERIDSTGIQGIGSIAWAVAERICRRFDRPDLADRCRIAMLRTL
                     KTAGPMQLGAIRVDHHRRMGQTR"
     gene            1726..>2289
                     /locus_tag="VFI12_03440"
     CDS             1726..>2289
                     /locus_tag="VFI12_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055487.1"
                     /translation="MTSRDAKIANVVAGLDRWLDATRVDWPTPGYGGPVIHWWNHCLA
                     YQGAGLDWRYEGIIDGYLTLWQRSGDAGWLDKARRAGDDLVCGQRADGHFTNSQFELN
                     PGIGGTPHEAAADVGLLLLARALRTIDDPAADRYLDAAVRNLEQYWFDRLWHEPTHTL
                     WDSPDTPSFVPNKAATFTEAVLLLAELT"
BASE COUNT          360 a          769 c          764 g          396 t
ORIGIN      
        1 gcggactctg ccggagaacc ctcctgatgg ttcagcagac cctctcgatc gcgcatgtga
       61 cggcaacctt tcccccgtat ttcggaggta cgggcaccgt gtgctttcac aacgcccgcg
      121 ttctggccag ccggggacat cgcgtttcgg tctacaccgc cgaatgggac ggccaatcgg
      181 acgatcctcc cggagtggag gtccaccggt tacggccggt agtacacgtc ggcaatgccc
      241 cggtgatgcc gcaactcctg cggatgccgc ggaatgcgat cgttcatctt catttcccgt
      301 tctatgccgg aggcgagctg gtttccctgt cgcgtcggcc atacgtcgtt acgtatcacc
      361 aggacgtcga gatcgacggg ctgctgggcc gcctgacctc cgcgcatgac gcaacgatcg
      421 ggcgacaggt gctccgtcgc gccaggcggc tctgcccgac cagcctcgac tacttccaat
      481 catcccggtt cgcgggtctg atcccggcga tggcgaaccg catctcccca attccgaacg
      541 gcgtcgatac gagcgtctat cggcctggtc cgcccgaccc ggcgctgcgg gctcggtatt
      601 cgctgcccga atcgatgccg atcgtgctct tcgtcggctc gatggaccgg gcacaccact
      661 tcaagggcat cccggtgctc ctgcaggcgc tggccactac cggcgacgtc gcggcgctgc
      721 tcatcggcga cggggatctg cgccggcaat acgagcagca ggctgagcag ctcggcgtcg
      781 cggaccgcgt ccggttcgcc ggccgggtgc ccgccgaaga tctgccggcc gcatatcgac
      841 tcgccgaggt tctgatcctg ccgagcgtga cccgcggcga ggcattcggg gtcgtcctcc
      901 tcgaagccat ggcgagcggt cgtccggtca tcgcgtcgga tctgcccggt gtgcgcagcg
      961 tcgtgtcaca cggcgtcgat ggcttcctga tcccgcccag cgatgccgca gcccttgcca
     1021 accagatcag cgagctgctg gcgctcgatc ccgccgttcg ggccgagatg ggcgcggcgg
     1081 gccggcgcaa ggtcgaggcg cactacgact gggagcgcat cggcgatcag ctcgaagcgc
     1141 tctatcgcgg cgtcctgcag gagacgagga gggtggcatg agcgagctga tccgcctgcc
     1201 cttcgaattg cgcggggcat tggattatgt cgcttttcgg accggcggcg tccggggaga
     1261 gaccggcgga cgcgtggtcg gcgatcccat actcgtcaac gagctcgggc gatacgcgcc
     1321 gatcgcgcct gctccggaga cggtcatcgt cttgctcggc cagtcagagg aaatcgccga
     1381 ggccgttgcg cagttatcgg ttaccgacgc ctggatcgtc acagccggcc ccctcgatcg
     1441 tctaatgcgc ccactgagga caaggacgac gggcgaaatt cggccgagtg cggccgtgcc
     1501 ggccgagcaa tggcgagcac tgggattcga gcgtatcgat tcgaccggga tccagggcat
     1561 tgggtcgatc gcctgggcgg tggccgagcg catctgccgg cggttcgacc ggcctgacct
     1621 tgccgaccgc tgccggatcg ccatgttgcg caccctcaag accgccggac cgatgcagct
     1681 cggcgccatc cgcgtcgatc accaccggcg catggggcag acccgatgac gagccgggac
     1741 gcgaagatcg cgaacgtggt cgccgggttg gatcgctggc tcgatgcaac gcgggtggac
     1801 tggccgactc cgggatacgg tggaccggtc attcattggt ggaaccattg cctcgcctac
     1861 cagggcgccg ggctcgattg gcgatacgag ggcatcatcg acggctacct gacgctctgg
     1921 cagcgatccg gcgacgctgg ctggctcgac aaggcccgtc gcgctgggga cgacctggtc
     1981 tgcggccagc gggcagacgg ccacttcacg aattcgcaat tcgagctcaa cccgggcatc
     2041 gggggaacgc cgcatgaagc cgccgcggat gtcggcctgc tccttctggc gcgtgcgctt
     2101 cggaccatcg acgacccggc ggcagatcgg tacctggatg cagccgtgcg gaacctggag
     2161 cagtactggt tcgaccggct ctggcatgag ccgacgcata cgctctggga cagcccggat
     2221 acgcccagtt tcgtcccgaa caaggccgcg acgttcactg aggccgtgct cctgctcgcc
     2281 gaactcacc
//
LOCUS       DASSGX010000195         2761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_472705, whole genome shotgun sequence.
ACCESSION   DASSGX010000195 DASSGX010000000
VERSION     DASSGX010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472705"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2761
                     /locus_tag="VFI12_03445"
     CDS             <1..>2761
                     /locus_tag="VFI12_03445"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET7055488.1"
                     /translation="LATSARFWAPSRQPWSFPQRFRNALIIDGIAILGILAAAMSLTS
                     RDANRLVGFAALAILAGLFLARCVRAYLMITGLEASVATRNTIIRLPGRAVNVQCEED
                     FAAILTEIAQGGATMVGMSRAEVHLGPSTGRAQPLVATWGLSDIEVDLLGRHPARESA
                     FPEARFGGPRPFVLTRSEAGLTIPAAHAWHVAGKESALVMPLRVGDRILGRVELWSPA
                     EPVQMTPALETMLTDFGGEAGLIVEHSQLVLAKRGDIIEAEMLLAVSEVFARATSLTE
                     CLPIVAERLLKRAESDRITISLIDHAAGEVFVAADARSLATREVHLQGKRWPLHIWVS
                     YDELVQRGSPIALARTDPAVSSGLARLMDLHGAQSYHAIPGFSDGTITSIVQIMSRST
                     ILRDSAEIRKIWTTIATQIQHGIEAVTALETSQRREQLESMRRRIKEALQVAGNLQDS
                     YGTIAEIFREIPDIDGVTILSWRSDHEHYVVEAEWTKPPIPCSPVGTMHEKDDYLLLQ
                     NIDQETAPRMRWVGQRGLSDDQQARLASYSIGAAMALPLRVEDQFVGAVMVYSVRNRA
                     FAADAVDWVQQLASTLSLVVRTISLRRHDEQVREEQTLKLRMLQAATSSSDPAEALMA
                     IAVAGLGSAGAESINIGFIDAERREMTIAADCTVPDWPGVEEPGTILALEAGSIQDRA
                     FQSLAPVTFRSDQPDLGKWDLEDIALYGAHAWIAAPLRVGGQGVGLLQVLARDPNAFD
                     SRRVQLWDEVAQQVSLLISSLQISFDNRRLLRQHQLVSRVSQAGAMASTREDAFRQLA
                     EIGLGLPGVGSSSIFLWHEDDELLEVAHEALGAEMVSRYRPGMRFQPPGLSGPGAPIR
                     IDGFSHLYVDKEDLTETERAMLAQLGVGRVAIFLLRDERQVHGVLSLKARETGPFPAE
                     TIRLAE"
BASE COUNT          454 a          923 c          911 g          473 t
ORIGIN      
        1 gctggcaacg tcggcccggt tctgggcacc gtcgcgacag ccatggagct ttccgcaacg
       61 attccgcaac gccctgatca ttgacggcat cgccattctc ggcatcctgg ccgccgccat
      121 gtctctcacg tcgcgcgatg ccaaccggct ggttggcttc gcggcgctgg cgatcctcgc
      181 cgggcttttc ctcgcccggt gcgttcgcgc ctatctgatg atcaccggac tggaggcgtc
      241 ggtcgcgacg cgcaacacga ttatccgcct gccgggccgg gccgtgaatg tccaatgcga
      301 agaggacttc gcggcgattc tgactgaaat cgcgcagggc ggagcgacca tggtcgggat
      361 gagccgggcg gaagttcacc tcggtccgtc caccgggcgc gcgcagccat tagtcgccac
      421 gtggggcctg agtgacatcg aggtggacct gcttggtcgt cacccggcaa gagaatcagc
      481 cttcccggag gcacggttcg gcgggccgcg tccgttcgtg ttgacgcgct ccgaggctgg
      541 tttgaccatt ccggccgcgc acgcctggca tgtcgcgggg aaggagtcgg cccttgtcat
      601 gccgctgcgg gttggcgatc gcatcctcgg ccgggtggag ctctggtcac cggccgaacc
      661 ggttcagatg acaccggctc tggaaacgat gctcacggac ttcggcgggg aggcggggct
      721 gattgtcgag cacagccaac ttgtcctggc caagcgcggc gacatcattg aggccgagat
      781 gctgctggcg gtcagcgaag tcttcgcccg ggcgacgtca ctgaccgagt gcttgccgat
      841 cgtcgccgag cggctgctca agcgggccga atcggaccgg atcacgatca gcctgatcga
      901 tcacgccgct ggggaggtgt tcgtcgccgc cgacgcgcgc agcctggcga ctcgtgaggt
      961 gcacctgcaa ggcaagcgct ggccattgca catctgggtc agctacgacg agctcgtcca
     1021 gcgcggcagc ccaatcgcgc tggcgcgaac cgatcccgca gtgtcgagcg ggctcgcccg
     1081 cctgatggac cttcacggcg cgcaaagcta ccacgcaatc ccgggcttct cggacggcac
     1141 gatcacctcg atcgtgcaga tcatgtctcg ctcgactatc ctgcgcgatt ccgccgagat
     1201 ccgaaagatt tggaccacca tcgccaccca gatccagcat ggcatcgaag ccgtcaccgc
     1261 cctggagacc agccagcgtc gggaacagct cgagtcgatg cggcgccgga tcaaggaggc
     1321 gctccaggtc gccgggaacc tgcaggacag ttacggcacc atcgccgaga tctttcgcga
     1381 gatccccgac atcgatggcg tcaccatcct cagctggcgg tccgatcacg agcattacgt
     1441 cgtcgaggcc gagtggacca agccgccgat tccctgctcg ccggtgggca cgatgcatga
     1501 gaaggacgac tatctcctcc tccagaacat cgaccaggag acggcgccgc gcatgcgctg
     1561 ggtcggacaa cgcggtctct ccgacgatca gcaggcgcgc ctggccagct actccatcgg
     1621 cgcggccatg gcgttgccgc ttcgcgtcga ggatcagttc gtgggcgccg tgatggtcta
     1681 ttccgtccgc aatcgcgcgt tcgccgcgga cgcggtggac tgggtccagc agcttgccag
     1741 cacgctttcg ctcgtggtcc gcaccatcag ccttcgccgc cacgatgagc aggtgcggga
     1801 agagcagacg ctcaagttgc gcatgctcca ggcggccacg tcgagctccg acccggccga
     1861 agcgctgatg gccatcgcgg ttgccggtct cggctcagcc ggggcggaat cgatcaatat
     1921 cggcttcatc gacgcggagc gtcgcgagat gacgatcgcc gccgattgca ccgtgccgga
     1981 ttggccgggg gtcgaggaac cgggaaccat cctggccctc gaggccgggt cgatccagga
     2041 ccgcgcattc cagtcgctgg cgccggtgac ctttcggagc gatcagccgg acctgggcaa
     2101 gtgggatctg gaagacatcg ccttgtatgg cgcccacgcc tggatcgccg cgccgctccg
     2161 ggtcggtggc cagggcgtcg gcctgctcca ggtgctcgcc cgcgatccaa atgcgttcga
     2221 tagccgacgc gtccagctct gggacgaggt cgcgcagcag gtctcgctgc tcatctcgag
     2281 cttgcagatt tcgttcgaca atcgccgcct cctgcgccag caccagctcg tctcgcgagt
     2341 gagccaggcg ggcgcaatgg catccacccg ggaagacgcc ttccggcaac tggccgaaat
     2401 cgggctgggt ctgccgggag tcggatcgag ctcgatcttc ctctggcatg aggacgacga
     2461 gctgcttgag gtggcgcatg aagccctcgg ggcagagatg gtgtcgcgct accgaccggg
     2521 catgcgcttc cagccgcccg gcctgagcgg cccgggcgcg cccattcgca tcgatggatt
     2581 cagccacttg tatgtcgaca aggaggacct caccgaaacc gagcgggcga tgctcgcgca
     2641 gctcggcgtc gggcgggtag ccatcttcct ccttcgtgac gagcgacaag tccacggcgt
     2701 cctttccctc aaggcgcggg aaaccggccc cttcccggcc gaaacgatac ggctcgctga
     2761 g
//
LOCUS       DASSGX010000196         4027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1127936, whole genome shotgun sequence.
ACCESSION   DASSGX010000196 DASSGX010000000
VERSION     DASSGX010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127936"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1182)
                     /locus_tag="VFI12_03450"
     CDS             complement(<1..1182)
                     /locus_tag="VFI12_03450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET7055489.1"
                     /translation="MAVIAQPARSTRTRPNSSSRRRKILAVFVVLLLVVGVAGGVYAW
                     RMVNAIVHAEKTAVYPLPPRGDQVAVQITPTNNPDTSNPVAGITEPTATMANVSAGGA
                     APEVTPTASYAALPTGDDPSRISVVQDLIGASVGSGDPGTSAVWGGKSAINLMVLGVD
                     RRENGGDQNADVIIIAHIDLITQKVAAVSIPRDLLVEIPGIGPDKINSAYNYGIKSDP
                     QSKVAGVVMMRDTIESVFGVMIDGYIMVDFGGFTDVIDAMGGVVIDVPTEIVDDQYPT
                     DDYGTEVIRFMPGRQFMDGDRALKYVRTRHQDSDDGRRARQLQVLHALFDKAKSFDSL
                     TNGFDIITALGGSVQTSFYLDQQLTLAQIGYAMNDEDIQLSSLTAPLIWGGYTDSGAW
                     VY"
     gene            complement(1301..2185)
                     /gene="proC"
                     /locus_tag="VFI12_03455"
     CDS             complement(1301..2185)
                     /gene="proC"
                     /locus_tag="VFI12_03455"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HET7055490.1"
                     /translation="MSTQTDQNQAETANSGCSGTIGFVGAGVMAESMIAGLLAKHVMR
                     PDQILVSHPRAERLEKLGERFGISVSQDNRTVAEQSDVVFLTVKPQVLWEVMPQLAGV
                     LTPQQVVVSIVAGATIETLEAGLLHNMIARVMPNTPAQVGAGMMVWAATTHVTEEQRQ
                     QVRTVLAALGEELYVDEEKYVDMATALSGTGPTYVFLMMEALIDAGVHMGFPRRIAEQ
                     IVLQTVGGSVEFARASRKHMAELRNMVTSPGGTSAEAIYQMEKGGLRTVFSRAVYAAY
                     LRTQTLAEEQSARRPRTS"
     gene            2318..>4027
                     /locus_tag="VFI12_03460"
     CDS             2318..>4027
                     /locus_tag="VFI12_03460"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET7055491.1"
                     /translation="MPNRLANEVSPYLQQHKDNPVDWYAWGTEALERAKAEDKPMLIS
                     IGYSACHWCHVMAHESFEDPATAELMNEHFINIKVDREERPDIDSLYMTAVQAMTGQG
                     GWPLNVFTTPEGVPFYGGTYWPPEDRMGMPGFRRVLDAVADTWANKREQIIEQGDQIR
                     AALAHRPSGSADGGDIGIESIERAAQHLGGTYDPLNGGFGGAPKFPQAPVLSFLLHAH
                     HLLDDRGSLTMAVETLEKMARGGIYDQIGGGFHRYAVDNVWLVPHFEKMLYDNAQLSQ
                     IYLDAYRITGQELFRRVAEQTLDYILREMTADQGGFYAAQDADSEGEEGKFYVWTPDE
                     IRAVLSAEDAELAERYFGVVEGGNFEGTSILRLAGNPDDYTEDVRRIRQELHEARAKR
                     VWPHRDEKIITSWNGMTIRALAEGGLVLGRPDFIQAAATCASFLKRDVDVNGVLHRSY
                     GGGSARIPAFLEDYAHLVDGLMRLYEATLDLAWLAWAQDLTARMVNLFVDEEEGGFYD
                     TSEQHDQLAIRPRELQDGATPSGSSVAADVLIRLGHVTSNDDMIQRGSDVLRSMRDFM
                     EDQP"
BASE COUNT          718 a         1258 c         1320 g          731 t
ORIGIN      
        1 atagacccag gcgccgctgt ccgtgtatcc gccccagatt agcggcgcgg tcaggctcga
       61 gagctggatg tcctcgtcat tcatcgcgta gccgatctgg gccagggtca actgctggtc
      121 caggtagaag ctggtctgga ccgagccgcc gagcgcggtg atgatgtcga agccgttcgt
      181 cagcgagtcg aacgacttcg ccttgtcgaa gagcgcatgc agcacttgca actgacgtgc
      241 ccggcggcca tcatcgctgt cctggtggcg ggtgcgcacg tatttgagtg cgcggtcgcc
      301 gtccatgaac tggcgcccgg gcatgaagcg gatgacctcg gtgccgtaat catcggtcgg
      361 gtactggtcg tcgacgatct cagtcgggac gtcgatcacg acaccgccca ttgcgtcgat
      421 gacatccgtg aagcccccaa agtcgaccat gatgtagcca tcgatcatga ccccgaagac
      481 tgactcgatc gtgtcccgca tcatcaccac gccggcgacc ttgctctgcg gatcggactt
      541 tatgccgtag ttgtaggcgc tgttgatctt gtccggtccg atcccgggga tctcgaccag
      601 cagatcgcgc gggatgctga ctgcggcgac tttctgggtg atgagatcga tgtgggcaat
      661 gatgatcacg tcggcgttct gatcgccgcc gttctcccgc cgatccacgc caagcaccat
      721 caggttgatg gcgctcttgc cgccccagac tgccgaagtg cctggatcac ccgatccaac
      781 cgacgcgccg atcaaatcct gcacgacgct gatccgggat gggtcatcgc cggttggcaa
      841 ggccgcgtag gacgccgtcg gcgtcacctc gggcgccgcg ccaccggcac ttacgttcgc
      901 catcgtcgcc gtcggttcag tgatgccggc gaccggattt gaggtgtccg ggttgttcgt
      961 tggtgtgatc tgaaccgcga cctggtcgcc gcggggcggc agcggataga cagcggtctt
     1021 ctcggcatgg acgatggcgt tgaccatccg ccaggcatag acaccgccgg ccactccaac
     1081 gacaaggagc aggaccacga agacggccag gatcttccgg cgtctcgaac tcgaattggg
     1141 acgcgtccgg gtggagcgcg cgggctgggc gattacggcc atgctttctc gcaggagtga
     1201 tggttcggca tctggtagct ggctgggacg aaggtgctgg ctcgtcccaa accggtcaag
     1261 tctacagtgc taacggagca tcccccgtga tggtttcgcc tcacgaggtg cgcggtcgcc
     1321 gggccgactg ctcttcagcg agcgtctggg ttcgcaggta cgccgcgtac accgcgcgtg
     1381 aaaagacggt tcgcagaccg cctttttcca tctggtagat ggcttccgcg gacgtgccac
     1441 ccggcgatgt aaccatattg cgcagctctg ccatgtgctt gcgcgacgca cgcgcaaact
     1501 cgaccgagcc gccgacggtc tggagcacga tctgctcggc aatccggcgt gggaaaccca
     1561 tgtggacacc cgcgtcgatc agcgcttcca tcatcaggaa tacgtatgtc ggaccggttc
     1621 cgctgagcgc ggtcgccatg tcgacgtatt tctcctcgtc gacatagagc tcctcgccga
     1681 gcgccgcgag cacggtccga acctgctgcc gctgctcctc ggtcacgtgc gtggtcgcgg
     1741 cccagaccat catgcccgcg ccgacctgcg ccggcgtgtt gggcatgacg cgggcgatca
     1801 tgttgtggag cagaccggcc tctagcgtct cgatggttgc gcccgccacg atcgagacga
     1861 cgacctgctg tggtgtcagc acaccggcga gttgcggcat gacttcccag agcacctgcg
     1921 gcttgacggt cagaaagacc acgtcggact gctcggcgac cgtgcggttg tcttgcgaca
     1981 cgctgatccc gaaccgctcc ccgagctttt cgagccgttc cgcgcgcggg tggctaacga
     2041 ggatttgatc gggccgcatg acgtgcttcg cgagcagccc ggcgatcatc gactcggcca
     2101 tgacgccggc gccgacgaag ccgatggtgc ctgagcaccc gctgttagct gtctccgcct
     2161 ggttctgatc cgtctgtgtg ctcatctgtc ctctgcgcta ggggaaatcc gcgatggcat
     2221 tgtagttgcg cgtgcgggcc atgtcccggg gagtgacagc gcggtgaacg gtagactggt
     2281 gggactagcg ctccgcgccg acgacaggag accacccgtg cccaaccgac tcgccaacga
     2341 agtcagccca tacctccagc agcataagga caacccggtc gactggtacg cgtggggaac
     2401 cgaggcgctc gaacgcgcaa aggcggaaga caagccgatg ctgatcagca tcggttactc
     2461 cgcctgccac tggtgccacg tcatggcgca tgaatcgttc gaagatccgg ccacggctga
     2521 gctgatgaac gagcatttca tcaacatcaa ggtcgaccgc gaagagcggc cggacatcga
     2581 ctcgctctat atgactgccg tgcaggcgat gaccgggcag ggcggctggc cgttgaacgt
     2641 cttcacgacg ccggaaggcg tgccgttcta cggcgggacc tactggccgc cggaggatcg
     2701 gatgggcatg ccgggcttcc ggcgggttct cgatgcagtg gccgatacct gggcaaacaa
     2761 gcgcgagcag attatcgagc agggcgacca gatccgggcg gctctggcgc accggccgag
     2821 cggttcggcg gatggcggcg acatcgggat cgagtcgatc gagcgggccg cacagcacct
     2881 cggtggcacg tacgatccgc tcaatggtgg cttcggcggc gcgcccaagt tcccgcaggc
     2941 gccggtgctt tccttcctgc tccacgcgca tcacctgctc gacgaccggg ggtcgttgac
     3001 gatggcggtc gagacgctcg agaagatggc ccggggcggg atctatgacc agatcggcgg
     3061 cggcttccat cggtacgccg tggacaatgt ctggctcgtg ccgcacttcg aaaagatgct
     3121 ctacgacaac gctcagctct cccagatcta cctcgacgcc taccgcatca ccgggcagga
     3181 gctcttccgc cgggttgccg agcagaccct cgactacatc ttgcgcgaga tgaccgccga
     3241 ccagggcggt ttctacgccg cgcaggacgc cgacagcgaa ggcgaggagg gcaagttcta
     3301 cgtctggacg ccggacgaga tccgggccgt cctctcagct gaggatgccg agctggccga
     3361 gcgatacttc ggcgtcgtcg aaggtggcaa tttcgaaggt acatcgatct tgaggctcgc
     3421 cgggaatccg gacgactaca cggaggacgt caggcgaatc cgccaagagc tccacgaagc
     3481 gcgggcgaag cgagtttggc cgcatcggga cgagaagatc atcacgagtt ggaatgggat
     3541 gaccatccgg gcgctggctg agggcggcct cgtactgggc cggccggact tcatccaagc
     3601 ggccgcgacg tgcgcgtcat tcctgaagcg ggatgtcgac gtcaacggcg tcctgcatcg
     3661 tagctacggt ggcggttcgg cccgaattcc tgccttcctg gaagactacg cgcacctcgt
     3721 tgacgggctg atgcggctgt acgaggcgac gctggatctc gcatggctcg cctgggcgca
     3781 ggatctgacg gcgcgcatgg tcaacctctt cgtggacgag gaagagggcg gcttctacga
     3841 cacgagtgag caacatgacc agctcgcgat ccggccacgc gagctgcagg acggtgcgac
     3901 accgtccggt tcatcagtag cggccgatgt cctgattcgg ttgggccacg tgaccagcaa
     3961 cgacgacatg atccaacgcg ggagcgacgt cctgaggtcg atgcgggact tcatggaaga
     4021 tcagccg
//
LOCUS       DASSGX010000197         2553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_582217, whole genome shotgun sequence.
ACCESSION   DASSGX010000197 DASSGX010000000
VERSION     DASSGX010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582217"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..1292)
                     /gene="metK"
                     /locus_tag="VFI12_03465"
     CDS             complement(96..1292)
                     /gene="metK"
                     /locus_tag="VFI12_03465"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009304567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HET7055492.1"
                     /translation="MRGSYLFTSESVSEGHPDKVCDRISDEIVDLFFREGPKAGVDPW
                     AIRAACETLATTNKVVIAGETRGPASVTNDHIEGVVRDAIKDIGYEQDGFHWKNCDIE
                     ILLHPQSADIAQGVDALQPGTNKEEGAGDQGIMFGYATNETPELMPAPIFYAHKILRL
                     ISEARHAGKEKVLGPDSKSQVTVQYEKGKPVGVREIVVSHQHLVEDMSSNQVRERVEP
                     YVRAALPKEWITDKTIWHINPTGKFFIGGPDGDSGLTGRKIIVDTYGGAAPHGGGAFS
                     GKDPTKVDRSAAYAARYVAKNIVAAGLADRCTLQLAYAIGVARPLSIYIDTHGTGKVP
                     EDTLERIVSEAMDLTPRGIRKHLDLNRPIYARTSSYGHFGRTPDNEGGFSWEKTDLAE
                     VIKRAV"
     gene            complement(1352..>2553)
                     /locus_tag="VFI12_03470"
     CDS             complement(1352..>2553)
                     /locus_tag="VFI12_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055493.1"
                     /translation="IEAYIAAGSPDGAAPVRPPEPVERRDPRANFRFFENRQKYLLFV
                     HTCSEKRVIADRVALEFGSIHPRPPALRVFDAGVGDGTVLARVMRSMHGRFPQMPFYI
                     AVKELSLEDIKLTLDKVPDRLFEHPATVMVLTNMYYAEAPWLAPRSPQAAAGLVWREV
                     ALRGTASGDFEAQIRDLQPFLEENWRASVSPVTGMPIYERPVVLVLYREDQQFQLDSV
                     IPRAGRAEANFDLVIASQPYRAKSSVNFRAKRIIAPLARGLRPGGRLIGIHSHGQDPG
                     LEIVQAVWPGENPFAVSRHELLRAVKYELGSAGRDLNFNAYADHRSIFRYDMEALPNE
                     VTGLIGTSTAFAAWNAAVYVAQVEDDRLTEMTQSGRTLDATREVLRKHNGLWFYDESY
                     VISRRRD"
BASE COUNT          426 a          834 c          824 g          469 t
ORIGIN      
        1 cgccacgaca atctgttggt tgggagggct gcccctcacc cgccgcgaag acgcggcgac
       61 ctctccccgc gagcggggag aggtgaaagg gaacatcaca ccgcgcgctt gatcacctcg
      121 gcgagatcgg tcttctccca ggagaagccg ccctcgttgt ccggcgtgcg gccgaagtgg
      181 ccgtaggacg aggtgcgcgc gtagatcggc cggttcaggt cgagatgctt gcggatgcca
      241 cgcggcgtca ggtccatcgc ctccgagacg atccgctcga gcgtgtcttc cggaaccttt
      301 ccggtgccgt gcgtatcgat gtagatcgac agcggccgcg ccacgccgat ggcataggcg
      361 agctgcaggg tgcagcggtc ggcgagaccg gccgcgacga tgttcttggc cacgtagcgc
      421 gcggcatagg ccgccgagcg gtcgaccttg gtcgggtcct tgccggagaa cgcgccgccg
      481 ccgtgcgggg ccgcgccgcc gtaggtgtcg acgatgatct tgcggccggt gaggccgcta
      541 tcgccatcgg gaccgccgat gaagaatttt ccggtcgggt tgatgtgcca gatggtcttg
      601 tcggtgatcc actccttcgg cagcgccgcg cgcacatagg gctcgacgcg ctcgcgcacc
      661 tgattggagc tcatgtcctc gacgagatgc tggtgcgaca ccacgatctc gcgcacgccg
      721 accggcttgc ccttctcgta ctgcacggtg acctggctct tggagtccgg ccccaacacc
      781 ttctccttgc cggcgtggcg ggcctcggag atcagccgca aaatcttgtg ggcgtagaag
      841 atcggcgccg gcatcagctc cggcgtctcg ttggtggcgt agccgaacat gatgccctgg
      901 tcgccggcgc cctcttcctt gttggtgccg ggctgcagcg cgtcgacgcc ctgcgcgatg
      961 tcggccgact gcggatgcag caggatttcg atgtcgcagt tcttccagtg aaacccgtcc
     1021 tgctcgtagc cgatgtcctt gatggcgtcg cgcaccacgc cttcaatgtg atcgttggtg
     1081 accgaggccg gcccgcgggt ttcgccggcg atgacgacct tgtttgtggt ggcgagcgtc
     1141 tcgcaggccg cgcggatcgc ccagggatcg acaccggcct tcgggccctc gcggaagaac
     1201 agatcgacga tctcgtccga aatccggtcg cacaccttgt cgggatgtcc ctcggacacc
     1261 gattcgctcg tgaacaaata agatccgcgc atcaatcgac ccttcatccg ccagaggtgg
     1321 cggtcctttc ttggctgtgt tccttggaat gtcagtcccg gcgacgcgag atgacgtagg
     1381 attcgtcgta gaaccagagc ccgttgtgct tgcgcaaaac ttctctggtg gcgtcgaggg
     1441 tgcggccgct ttgagtcatc tccgtcaacc ggtcatcttc gacttgggcg acgtagaccg
     1501 ccgcgttcca cgctgcgaaa gccgtcgagg ttccgatcag cccggtgacc tcgttgggca
     1561 gcgcttccat atcatatcta aagatcgaac gatggtccgc gtatgcgttg aaattgaggt
     1621 cgcgccccgc ggaaccaagt tcgtatttca ccgcgcgcaa taactcatga cggctcacgg
     1681 cgaaagggtt ttctccgggc cagaccgcct ggacgatctc cagtccggga tcctgtccat
     1741 gagaatgtat tcctattaaa cggccgccgg gccgcaggcc gcgcgccagc ggcgcaatga
     1801 tcctcttggc ccggaaattc acggaagatt ttgctcgata gggctgggag gcgatcacca
     1861 gatcgaaatt ggcctcggcc cgtccggccc gcggaatcac cgaatcgagc tggaactgct
     1921 ggtcctcccg gtacagcacc agcacgaccg gccgctcata gatcggcatc cccgtgaccg
     1981 ggctgacgct ggcccgccag ttctcctcca gaaacggctg gagatccctg atctgggcct
     2041 cgaagtcgcc ggaggcggtt ccccgcagcg ccacctcacg ccagacaagg cccgccgcgg
     2101 cctgcggcga gcggggcgcg agccacggcg cctccgcgta atacatgttg gtcagcacca
     2161 tcacggtggc cggatgctcg aacagccggt ccggcacctt gtcgagggtc agcttgatgt
     2221 cctcgaggct gagttccttg acggcgatgt agaacggcat ctggggaaac cggccatgca
     2281 tggaccgcat cacccgcgcc agcacggtgc cgtcaccgac gcccgcatcg aacacacgca
     2341 gcgccggcgg acgcggatgg atgctgccga actccagggc gacgcggtcg gcgatcaccc
     2401 gcttctcgct gcaggtgtgg acgaacaaca gatacttctg ccggttctcg aagaaacgga
     2461 agttggcgcg cggatcgcgc cgctcgaccg gttccggcgg ccgaaccggc gccgcgccat
     2521 cgggcgagcc cgccgcgata taggcctcga tcc
//
LOCUS       DASSGX010000198         2111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_256102, whole genome shotgun sequence.
ACCESSION   DASSGX010000198 DASSGX010000000
VERSION     DASSGX010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256102"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VFI12_03475"
     CDS             complement(<1..273)
                     /locus_tag="VFI12_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055494.1"
                     /translation="MTVCTRYWLFQVLGWGGYSILGVTIAAFNVGWSVRLVTDFALYF
                     VYSIALTDALRQIITRGRWLNPPIRLVWLRLASAILVIAAIQVTLIV"
     gene            complement(270..704)
                     /locus_tag="VFI12_03480"
     CDS             complement(270..704)
                     /locus_tag="VFI12_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055495.1"
                     /translation="MKTLLLALALTIAPLIPASASSDPFIVSVIGRGRPMILIPGLSS
                     GPETWTTTFDPSAVADAMGEMFGVNLEPAPARITAPTLVLAAWQSYAQYTNHDRHMAT
                     LRQQFTNLHGVQLQLNDTAHHFIMWDDPRWLFEQMDRFLATR"
     gene            complement(830..1768)
                     /locus_tag="VFI12_03485"
     CDS             complement(830..1768)
                     /locus_tag="VFI12_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055496.1"
                     /translation="MKWLRMITFACVAAMTVPVYAQWPKHLNPGVPLKDGKPDLDAPA
                     PKAPDGHVDLSGVWETFTPRPAGAPQRGGGPRGAGPQRGGTPPPAPARGAGTPAASGQ
                     VAARGTGQLPPGGGNPFGNIGIQQPGREAPYQPWASDLVKTRMADNSKDNPDAHCLPM
                     GVMQMTSHPYPKKIIQTPTEVLMIYEASGTTVREIFLDRPLPKAEDVEPFWNGYSVGH
                     WEGDTLVVETTGFMDDGWLDVRGSPLTNQGKLTERYRRPSMGRLEIEVTVDDPKAYTK
                     PFTATVQNRLLPDTQLIEFVCLDKDAQHYVGGDSPK"
     gene            1834..>2111
                     /locus_tag="VFI12_03490"
     CDS             1834..>2111
                     /locus_tag="VFI12_03490"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7055497.1"
                     /translation="MTEPAYEVYALKYAHHDRHSRENFLVEDPHDSLSPLAYYVWVIV
                     GGGRTILVDTGFDERMARKRGRTITMPVARGLGALGIDAASIEDIILT"
BASE COUNT          363 a          653 c          719 g          376 t
ORIGIN      
        1 cacgatcagc gtgacctgga tggcggcaat gaccaggatc gctgacgcaa gacgcagcca
       61 cacgaggcgg atcggcgggt tcaaccaacg tcccctggtg atgatctggc gtagtgcatc
      121 cgtcagcgcg atgctgtaga cgaagtagag cgcgaagtcc gtgaccaggc gcacgctcca
      181 gccgacgttg aacgcggcaa tcgtgacacc gaggatggag tagccgcccc agcccaagac
      241 ctggaacagc cagtagcgcg tgcagacagt catcgcgtgg cgaggaatcg atccatctgc
      301 tcgaagagcc agcgcggatc atcccacatg atgaagtgat gggcggtgtc gttcagttga
      361 agttgaacgc cgtgcaaatt ggtgaactgc tgccgcagtg tggccatatg ccgatcgtgg
      421 ttcgtgtact gcgcgtagct ttgccaggcg gcgagcacga gcgtcggcgc ggtgatgcgc
      481 gcgggcgccg gctcgaggtt gacgccgaac atctcaccca tggcatcagc cacggcagac
      541 ggatcgaagg tggtggtcca ggtctcgggg ccggaggaga gacccgggat gaggatcatc
      601 ggccgcccgc gaccgatgac cgagacgatg aatgggtccg acgaagcgct tgccgggatg
      661 agcggcgcga tcgtcaacgc gagcgcaagc aacagtgttt tcatgcgccg agcatcgtgc
      721 tggcgcgctc gcggttcggc gggattgtga tgagcggtgc tggcgcgtga taggtgcgga
      781 gagggcggag gtagcggatc tccgcctctc agctcctccg ccttggctgc tacttcgggc
      841 tgtctccccc gacatagtgc tgcgcatcct tatcgaggca gacgaactcg atcagttgcg
      901 tgtcgggcag caggcggttc tgcacggtcg cggtgaatgg tttcgtgtag gcctttggat
      961 cgtcgacggt gacttcgatc tcgaggcggc ccatgctggg acgacgataa cgctcggtca
     1021 acttgccttg attcgtcagt ggactgccgc gcacgtcgag ccagccgtca tccatgaagc
     1081 cggtggtctc gacgacgagc gtgtctcctt cccaatgccc gacggagtag ccgttccaga
     1141 acggctcgac gtcttccgcc ttcggcagcg gacggtcgag aaagatctcg cgcacggtgg
     1201 tccccgaggc ctcgtaaatc atcaagacct cggtcggcgt ctgaatgatc ttcttgggat
     1261 agggatgcga cgtcatctgc atcacgccca tcggcaggca gtgcgcgtcg ggattgtcct
     1321 tgctgttgtc ggccatccgc gtcttcacga ggtcggatgc ccagggctga tacggcgcct
     1381 ctcgaccggg ctgctggatg ccgatgttgc cgaagggatt cccgccgccg ggcggaagct
     1441 gtccggtgcc ccgtgcggcc acttgtccgg acgcggccgg agtaccggcg ccccgcgctg
     1501 gtgctggtgg tggcgtgccg ccgcgctgcg gtccggcacc gcgcgggcct ccgccccgct
     1561 gcggcgcacc ggcgggacgc ggcgtgaacg tttcccacac gcccgagaga tcgacgtgac
     1621 cgtcaggagc tttcggggcc ggtgcgtcga ggtcgggctt gccgtctttc agcggcacac
     1681 cggggttgag gtgtttgggc cactgcgcgt acaccggcac ggtcatcgcc gccacgcacg
     1741 cgaacgtgat catccgcagc catttcataa gggcctcaat atacgcgatg gccggggcgc
     1801 cggagccaat cgccttcggg taaactgcgc cgcatgaccg agccggcgta cgaggtgtac
     1861 gccctcaaat acgcgcacca cgaccggcat tcgcgcgaga acttcctggt cgaggaccct
     1921 cacgacagcc tctcgccgct ggcgtactac gtgtgggtca tcgttggcgg cggccgcacg
     1981 atcctggtcg ataccggctt cgacgaacgc atggcgcgca aacggggccg cacgatcacc
     2041 atgccggtcg ccaggggcct cggcgcgctc gggattgatg cggcgtcgat cgaagacatc
     2101 atcctcaccc a
//
LOCUS       DASSGX010000199         4564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_582854, whole genome shotgun sequence.
ACCESSION   DASSGX010000199 DASSGX010000000
VERSION     DASSGX010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4564
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582854"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="VFI12_03495"
     CDS             <1..950
                     /locus_tag="VFI12_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HET7055498.1"
                     /translation="PSDVDQVLLVGGMTRMPRVQQKVKEFFGKEPHKGINPDEVVAIG
                     AAIQGGVLGGEVKDILLLDVTPLTLSIETMGGVATPLIERNTTIPTRKSQVFTTAAEN
                     QPQVEINVLQGERPMAADNKSLGKFILDGILPAPRGVPKIEVTFDIDANGILNVTAKD
                     QATSKEQKITITGSSGLSDTEVDRMVHEAEEHAEEDRKRREAISLRNDAESMVFQAEK
                     TLTDYGDQIPVEIKTELDEKIQTVKEILDKDRENVDRLKPAYEEMVQTLSKVGSAMYG
                     QAGAASPNGGEGFPGGGAEETPEDESTVEGEFREVGSER"
     gene            complement(1005..3299)
                     /locus_tag="VFI12_03500"
     CDS             complement(1005..3299)
                     /locus_tag="VFI12_03500"
                     /inference="COORDINATES: protein motif:HMM:NF037144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HET7055499.1"
                     /translation="MTALIAAVSTVLVVTTPAAQAAKAAEPDTVAASINTIATFDVLA
                     NDPRGTTLVDHTTPAHGHLKRLESGKFTYLPDANYLGTDAFNYRAQRGTDEVQTVDVS
                     IEVSGRVTSVECRDDSLATDEDVAAIVYVLANDGHGPDGLPLTVTVDQQPGNGTAEVQ
                     PDNSILYSPAANWSGSDSFTYVASDGSTRETASVSIDVAPINEPPTAVDDTAVTRQDG
                     SASIDVLANDSDADGDEFGFVGNSQPMYGKATWSNVDHMIIYEPNDGFWGTDQFTYSI
                     GDFDGVTTGTVTVTVNGAPAPQPDSALTDRGNPVDIPVLLNDTDPDGDALSVTSVGAA
                     EHGDVLINPDATVRYVPIPDFTGQDTFTYTVRDERGNARDAAVTVSVAQGAVRASGAT
                     DDEYLIIEDSPGNTLDVLSNDSVPGGTTPAISIDTTPAHGTLALDAAQNITYVPDPGF
                     SGNDSFTYSISDGVNPRSTAMVSLEIQPANDAPVAAADSATGDEDTEIAIDPLANDTD
                     EEGDDLTVESVTQPANGVASVRRDGTVVYVPAADFAGFDSFDYVLSDGNGGTATATIT
                     ITVNPVNDPPNPADDNARTDIGVATDIDVLANDHDPDGPQTLLSITKHPKHGSAIVTT
                     SNTILYTPEPKFEGYDTIEYQHSDGLAAASATVDVAVGNPNSAPVAKDDEADTAQGQP
                     VTIAVLKNDRDRDDDDMSLYGLTDPAHGTISVDKGVVTYTPDPNFVGTDSFTYKVRDG
                     KGGRDKAEVRVTVNPAHDRRINDD"
     gene            complement(3566..>4564)
                     /locus_tag="VFI12_03505"
     CDS             complement(3566..>4564)
                     /locus_tag="VFI12_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055500.1"
                     /translation="PRHLAVRITIADQLGISPHDVAKHMLRPVGSAFAAGEAMAKTRK
                     GLRNTVVASPVSGTLLSIDPDTGVGLLAPGAGGDIRSLVSGDVEYVDGKQSIAIRTVG
                     SRMFGIVGVGPSVEGALCLAVGGPGEELQPDKVTPDMRGRIVVAGASVSAATLRRLLD
                     AGASGVVVGGIIEREVSACFGVPAEDRLAAWRIGPSDTGIGDNMMTTIAIVATEGFGT
                     IPISGDAFSYLKRFDNQQVTLLTTTRVNGFLARPQIIHVNQDALDDDAPAKPVALTTD
                     TRVRLVDQAHLGIAATVADRPKRVRRADGMNVDVITVATSDGTTRTVAANNVEILA"
BASE COUNT          793 a         1393 c         1532 g          846 t
ORIGIN      
        1 aaccttcgga cgtggaccag gtgctgctgg tgggcggtat gacccgcatg ccgcgcgtcc
       61 agcagaaggt gaaggagttc ttcggcaagg agccgcacaa gggcatcaac ccggacgagg
      121 tcgtcgcgat cggcgccgcg atccagggtg gtgtgctcgg cggcgaggtc aaggacatcc
      181 tgctcctcga cgttactccg ctgaccctgt cgatcgagac gatgggcggc gtggctactc
      241 cgctaatcga gcgcaacacg accattccga cgcgcaagag ccaggtcttc accacggcgg
      301 ccgagaatca gccgcaggtc gagatcaacg tgctccaggg cgagcggcca atggcggccg
      361 acaacaagtc gctcggcaag ttcatcctgg acggtatcct gccggctccg cgcggcgtgc
      421 cgaagatcga ggtgaccttc gacatcgatg cgaacggcat cctcaatgtc accgccaagg
      481 atcaggcgac cagcaaggag cagaagatca cgatcaccgg ctcctccggc ttgtctgata
      541 ccgaggtcga ccggatggtc cacgaggccg aagagcatgc cgaggaggac cgcaagcggc
      601 gcgaggcgat ctcgctgcgg aacgatgccg agagcatggt cttccaggcc gagaagacgc
      661 tgaccgatta cggcgatcaa atcccggtcg agatcaagac cgagttggac gagaagatcc
      721 agacagtcaa ggagatcctc gataaggatc gcgagaatgt cgatcggctc aagccggcgt
      781 atgaggagat ggtccagacc ctctccaagg ttggctccgc gatgtacggc caggcgggcg
      841 cggcgtcacc gaatggtggc gagggcttcc cgggcggcgg cgcggaagag acgccggaag
      901 acgaatcaac cgtcgagggc gagttccgcg aggtcggttc ggagcggtaa tccgcgtcga
      961 tcaacgtacg aaatcgagac tgaggggcgc agttactgcg cccctcagtc gtcgttgatg
     1021 cggcgatcgt gggctggatt gacggtgacg cgaacttcgg ctttgtcgcg gccacccttg
     1081 ccgtcgcgaa ccttgtaggt gaacgagtcg gtcccgacga agttcggatc gggcgtgtag
     1141 gtgacgacgc ctttgtcgac tgagatcgtt ccgtgcgccg gatcggtcag gccgtagagg
     1201 gacatgtcat cgtcgtcccg gtccctgtcg ttcttcagca cggcgatcgt cactggttga
     1261 ccctgtgcag tgtcggcctc gtcatccttc gcgaccggcg ccgagttggg gttgccgact
     1321 gccacgtcga cagtcgcgct ggcggccgcc aggccatcgg agtgctggta ttcgatcgtg
     1381 tcgtacccct cgaacttcgg ttccggcgtg tacaagatcg tgttggacgt ggtcacaatc
     1441 gccgatccgt gcttcggatg cttcgtgatc gagagcaggg tttgcggacc atccggatcg
     1501 tgatcgttgg cgaggacatc gatgtcggta gcaactccga tatccgtgcg ggcattgtcg
     1561 tccgccgggt tcggcgggtc gttgaccgga ttcacggtga tcgtgatggt cgcggtcgct
     1621 gttccgccgt tgccgtccga gaggacataa tcgaacgaat cgaatccggc gaaatcggcc
     1681 gccgggacgt agacgacggt gccgtcgcgc cgaacgctcg cgactccatt ggcgggctgg
     1741 gtgaccgact cgaccgtcag gtcgtcgcct tcctcatcgg tgtcattcgc gagcggatcg
     1801 atcgcgatct ccgtgtcctc gtcgccggtc gctgagtccg ccgccgcgac cggcgcgtca
     1861 ttggccggct ggatctcgag cgagaccatt gcggtcgagc gcgggttgac gccgtcgctg
     1921 atcgaataag tgaacgagtc gttccccgag aagccgggat cggggacgta ggtgatgttc
     1981 tgcgccgcgt cgagcgcaag cgtgccatgc gccggtgtcg tgtcgatcga gatcgcggga
     2041 gtcgtgccgc ctggcacgga gtcgttggac agcacgtcca gcgtatttcc gggcgaatcc
     2101 tcaatgatca gatactcgtc gtcggtggcc ccgctggccc ggaccgcacc ctgcgccacg
     2161 ctgaccgtga ccgccgcatc ccgcgcattc ccgcgctcat cccgcacggt gtaggtgaac
     2221 gtgtcctgac cggtgaaatc tggaatcggc acgtagcgga ccgtcgcgtc cgggttgatc
     2281 agcacgtcgc catgctccgc ggcgcctacg cttgtgacgg agagcgcgtc gccgtccgga
     2341 tcggtgtcgt tgagaaggac cgggatgtcg acgggattgc cccggtcggt gagcgcgctg
     2401 tccggctgcg gggccggcgc tccatttacc gtcacagtga ctgttccggt cgtgaccccg
     2461 tcgaagtcac cgattgaata ggtgaactga tcggtgcccc agaaaccgtc gttgggctcg
     2521 tagatgatca tgtggtcgac gttggaccag gtcgccttgc catacatcgg ctgcgaattg
     2581 ccaacgaatc cgaactcgtc gccatccgcg tcactgtcat tggcgagcac atcgatcgag
     2641 gcggacccat cctgtcgggt cacggcggta tcatcgacgg cggttggtgg ctcattgata
     2701 ggcgccacat cgatcgagac cgaagccgtt tcccttgtcg aaccatccga cgcaacgtac
     2761 gtgaacgagt cggagccgga ccagttcgcg gcgggcgagt agaggattga attgtccggc
     2821 tggacctcag ccgtgccgtt gcccggttgc tgatcaacgg tcaccgttaa cgggagtccg
     2881 tcgggtccat ggccgtcatt ggcgagcacg tagacgatcg ccgccacgtc ctcatcagtc
     2941 gcaagcgagt catcccggca ctcgacagat gtgactcgac cggagacttc gatggagacg
     3001 tcaaccgtct ggacctcgtc ggtcccgcgt tgtgctcgat agttgaaggc atccgtgccc
     3061 aggtagttgg cgtccgggag atacgtgaac ttaccgctct caaggcgttt caggtggccg
     3121 tgcgccgggg tcgtgtgatc gacgagtgtt gtcccgcgcg ggtcattggc caggacatcg
     3181 aatgtggcga tcgtgttaat gctggccgcg accgtgtccg gttcggccgc cttggcagcc
     3241 tgtgccgcgg gggttgtgac gacaagcacg gtggacactg cggcaataag cgcagtcacc
     3301 cggagggcct gaagcgggcg ccgacgggcg cgaatccgcg ccattgcgtc agcgaatggg
     3361 ccggcgggcg ccagacgttc ccggggttgt ctcggcatgg tatctgttgc tttccggtgg
     3421 tcgtacacgt ccttctacag cgtacccggt ctgagcccat actccaataa ccagtcggtc
     3481 cggttttggg gacccaggag tgctcaaatg cgaagcggcg ccgcgactac cgcggcgccg
     3541 ttcgattacg tgttgtcgat caagctcaag cgaggatctc gacgttattg gcggcaacgg
     3601 ttcgcgtggt gccatcgctc gttgcgacgg tgatcacatc gacattcatg ccgtctgccc
     3661 ggcgcacccg cttcggcctg tcggcgaccg tggcagcgat gcccaggtgg gcctgatcaa
     3721 cgaggcgcac ccgcgtgtcc gtggtgagcg cgactggctt ggccggggcg tcatcatcca
     3781 gcgcgtcctg gttgacgtgg atgatctgcg gccgggccag gaatccgttg acgcgcgtcg
     3841 tagtgaggag cgtcacctgc tggttgtcga atcgcttgag gtaagaaaag gcgtcgccgc
     3901 tgatcggaat cgtgccaaac ccttcggtgg cgacgatcgc aatcgtggtc atcatgttat
     3961 cgccgattcc ggtatccgat ggcccaatgc gccacgccgc gaggcgatcc tcggccggga
     4021 cgccgaagca cgccgacact tcccgctcga tgattccgcc gacgaccacc ccagacgcgc
     4081 cagcatccag cagccggcgc agagtcgcgg cgctgacgct ggcgccggcc accacgatcc
     4141 tgccgcgcat gtccggcgtc actttgtccg gctgcagctc ctctccgggg ccaccgacgg
     4201 ccaggcagag cgcgccttcg accgaaggac cgacaccaac aatgccgaac atccgcgaac
     4261 cgacggtgcg aatggcgatc gactgcttgc cgtcgacgta ttccacgtcg ccggaaacga
     4321 gcgagcggat gtcgccgccg gctccgggcg caagcaggcc gacgccggtg tcgggatcga
     4381 tcgacagcag cgtgccgctc accggtgacg cgacgacggt gttccggagt cccttacgtg
     4441 tcttggccat tgcctcgcca gcggcaaagg cggagccgac cggtcgcagc atgtgcttgg
     4501 cgacgtcatg cggtgagatg ccgagttgat cggcgatcgt gatccggacg gcaaggtggc
     4561 gcgg
//
LOCUS       DASSGX010000200         2153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_691977, whole genome shotgun sequence.
ACCESSION   DASSGX010000200 DASSGX010000000
VERSION     DASSGX010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691977"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..849)
                     /locus_tag="VFI12_03510"
     CDS             complement(160..849)
                     /locus_tag="VFI12_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HET7055501.1"
                     /translation="MSTEPPATKQVTHAMEDYLKAIYRLSERDPEVTPQQIANELSVS
                     GASVTNMVKRLDDLNLVDYSRYKGVTLTDSGRKIALEVIRHHRLLELYLSESLGMPWH
                     EVHAEAERLEHHLSDELEARMDSALGFPTHDPHGDPIPSPDLVIDEVRGVNLTALTPG
                     QKGTVTRVSDRDPAQLEYLGSLGLYPGAGVELIERLPFEGPLRIRVAGKDAVLGPPLA
                     RQVSIEPSTES"
     gene            1001..1762
                     /locus_tag="VFI12_03515"
     CDS             1001..1762
                     /locus_tag="VFI12_03515"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HET7055502.1"
                     /translation="MAEKKWDWRTDSVLAKGPTRVAVIAAHPDDPDHGCAGSCAVWSD
                     MGHHVTYVIVTNGNKGSDDIHLSSADLVATREREQSAAAEILGLKGGVLFLGKDDGMV
                     VPDLELRRDIVRVIRKLKPDVILCESPDSWFYGDSYINHPDHRAVAQATLAAIFPASQ
                     NHRYFPELLAEGFEPHRVREVWIDSISGQDDAIVDVTSVADRKVEALRAHKSQMVGGD
                     PDEWIREWMRENGKKHSPPVEYAESFKVMRINGDE"
     gene            1869..>2153
                     /locus_tag="VFI12_03520"
     CDS             1869..>2153
                     /locus_tag="VFI12_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid aminotransferase"
                     /protein_id="HET7055503.1"
                     /translation="MAVKTTMLDYAYFEGNVVPFGEATISIGTHAIQYGTGAFAGIRG
                     YVDQDGETINIFRLIDHTTRLLNSGKLLRSELDYTPESLAEVIANLVAKNA"
BASE COUNT          403 a          727 c          659 g          364 t
ORIGIN      
        1 tccctccgcc atcgcgtcgg cgatccggat gccaacctga gccgcgaagc catctccgcg
       61 ggcctgatca acgatcccct cccccgccca acaccccccc gccaactccc gccagacggc
      121 aggtaggcac cggcaagcgc ccgtagtggc tgggcgtcgt tatgattccg tactgggctc
      181 gatcgagacc tggcgggcga gcggcgggcc gaggacggca tccttgccgg caaccctgat
      241 ccggagcggg ccctcgaacg gcagccgctc gatcagctcc acccccgcgc cgggatagag
      301 accgagcgag ccgagatatt cgagctgggc cgggtcacga tccgagacgc gcgtgaccgt
      361 ccccttctgc cccggcgtca gcgcggtcag attcacgccg cggacctcgt cgatgaccag
      421 gtcggggctc gggattggat cgccatgggg atcgtgcgtc ggaaagccga gcgccgagtc
      481 catccgcgct tccagctcgt ccgagaggtg gtgctccagc cgctcagcct cggcgtggac
      541 ctcgtgccac ggcatcccga gcgactcgga gagatagagc tccagcaatc gatgatgccg
      601 gatcacctcg agcgcgatct tccggcccga atcggtcagg gtgacgccct tgtaccggga
      661 atagtcgacg aggttcaggt cgtcgagccg cttgaccata ttcgtgaccg acgcgccgct
      721 caccgagagc tcgttggcga tctgctgcgg cgtcacctcg ggatcgcgct cgctcaggcg
      781 atagatcgcc ttaaggtaat cctccatggc gtgcgtgacc tgctttgttg ctggtggttc
      841 ggtgctcatg tccccgtccg gttgtcgtca acggagttcg ctggctccat cctgatcaac
      901 gtgccggtat cctgtcaaat cctgcaggcc gacattcacg cccgattgct ccattattcc
      961 cgcatgtgat ctgtctgatg atgaaggaga gcaaccccgg atggctgaga agaagtggga
     1021 ttggcgaacg gactcggttc tggccaaggg gccaacccgt gtcgcggtga tcgccgcgca
     1081 tcccgacgat ccggatcacg gctgcgccgg ctcgtgcgca gtctggtccg acatgggtca
     1141 ccacgtgacc tacgtgatcg tcaccaacgg caacaaaggc agcgacgaca tccacctcag
     1201 ctcggccgac ctcgtcgcca cccgcgagcg ggagcaaagc gcggcggccg aaatcctcgg
     1261 gctaaagggc ggcgtcctct tcctcggcaa ggacgacggc atggtcgtgc cggatctgga
     1321 gctgcgccgc gatatcgtcc gggtcatccg caagctgaaa cccgatgtca tcctctgcga
     1381 gagccccgat tcctggttct atggcgacag ctacatcaac cacccggacc accgcgctgt
     1441 ggcccaggcc acgctcgcgg ccatcttccc cgcctcccag aaccaccgct acttcccgga
     1501 gctgctcgcc gagggctttg agccgcaccg ggtgcgcgaa gtctggatcg actcgatcag
     1561 cggccaggac gatgccatcg tcgatgtcac ctccgtggcc gaccgcaagg ttgaggcgct
     1621 acgggcgcac aagagccaga tggtcggcgg cgaccccgac gagtggatcc gcgagtggat
     1681 gcgggagaac ggcaagaagc acagcccgcc ggtcgaatac gccgaatcat tcaaggtgat
     1741 gcgaatcaac ggggacgaat agtcaccgtg ccgagcggta accacgctgt gcaataatcc
     1801 ggagcaagga tttcgaggca catcaccggc gccgcgccgg ttcatacgcc aaacgaagga
     1861 agccgcacat ggccgtcaag acaacgatgc tcgattacgc atacttcgag gggaatgtcg
     1921 ttccctttgg agaggccacc atcagcattg gcacccacgc cattcagtac ggcaccggcg
     1981 cttttgccgg cattcgcggc tacgtcgacc aggacggcga gacgatcaac atcttccgcc
     2041 tcatcgacca cacgacccgg ctgctcaatt ctggcaagct gctccgctcc gagttggact
     2101 atacgcccga gtcgctcgcc gaggtgatcg ccaacctcgt cgcgaagaac gcg
//
LOCUS       DASSGX010000201         4132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1236360, whole genome shotgun sequence.
ACCESSION   DASSGX010000201 DASSGX010000000
VERSION     DASSGX010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1236360"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VFI12_03525"
     CDS             <1..307
                     /locus_tag="VFI12_03525"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HET7055504.1"
                     /translation="DAMVLGSSYSPYGRRQAPPSIPIIVYGTRWCAQSQLVRRYLDRL
                     GIPYEFVDLERDPEAVRRLRWLTGGEARHPTVYIGGEWLVEPTTSELGWALTRSGLR"
     gene            476..1435
                     /locus_tag="VFI12_03530"
     CDS             476..1435
                     /locus_tag="VFI12_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055505.1"
                     /translation="MQTHSGRPAAARPALIMMALGVLVAASMLDGGSGQSLAQEGAAT
                     PATPVLATPLAIDFTQPVAERPAAIMSGSCDDPGEVIAPLTALEHPEGEAQGQGAAIE
                     AARSYTSLPIDLTSLLEGGASVNILFSDEEPETVIACGEIGGTQGESGSIVIKLSPRN
                     GSGYSGIAFLLPGDDGTTGISVFVAGEQTVADTHEVAAVASPGPGLIPLEPAEATAVP
                     TPTAEPVQVVDVALLEWLIDMPAEIRAGQINFAITNEGTEAHSFTIESGGVVVAELDQ
                     PIDPGDSTVLSVTLAEGEYDVYCPLGDGEHREKGMETTLTVTP"
     gene            1599..2081
                     /locus_tag="VFI12_03535"
     CDS             1599..2081
                     /locus_tag="VFI12_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055506.1"
                     /translation="MPDWFARMSWESRNKNQLTVISDSPASYVGLLSLCGIVACVALA
                     LGLWQRRWVVAAMLVAAGAFAYAAYVSGSYWLGLSRGVMLLDGRLVQDMGSEMTAHWP
                     RALPLFVIAAIAGAISALALTVYWREALRATRCWGARREANCHSRGGKRSTPKMSARI
                     "
     gene            complement(2030..2566)
                     /locus_tag="VFI12_03540"
     CDS             complement(2030..2566)
                     /locus_tag="VFI12_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055507.1"
                     /translation="MSDERRDADQLAATFSDPADALSRLNAYCWFGQAERVYFNAREQ
                     LTIDVSIHDFSGSEGATLAQRWFGYQRANDLHLQQEVKTRAQLEEELNDAGLGKVNIE
                     TLDDVVIYALYNGTEYTLYARRDPDTGIQNGSPLPSNILVRVSVSGGTTASGMSRQVV
                     AYQILADIFGVDLFPPRE"
     gene            3207..3542
                     /locus_tag="VFI12_03545"
     CDS             3207..3542
                     /locus_tag="VFI12_03545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HET7055508.1"
                     /translation="MADSRPATRTVTVADIEGQLAPLLRDIDRGELRVLVEQEGLPIA
                     AIISVRDLERFDRLERERDERFAVIDRMREAFADVPIEEIEREAERSVAEARDRLRRR
                     ADELTARSA"
     gene            3539..3961
                     /locus_tag="VFI12_03550"
     CDS             3539..3961
                     /locus_tag="VFI12_03550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HET7055509.1"
                     /translation="MIVAVLDTNVLVSGFPAPTGVPGTLIDLWRRGVYRLVVSEPILM
                     ELAETWRDPYWAARFPSDESKAAIDLLRDMALVTPLTVEVAGVATHPEDDLILATAVS
                     GGADYLVTGDRKVLAIGAFGNVTIFSPRAFLTRLAEDA"
BASE COUNT          709 a         1270 c         1380 g          773 t
ORIGIN      
        1 tgatgcgatg gtccttggca gcagttattc cccctacggt cggcggcagg cgcccccgag
       61 tattccgatc atcgtctatg ggacgcggtg gtgcgcgcag agccagttgg tgcggcgcta
      121 tctggaccgg ttggggattc cgtacgaatt cgtcgacctc gagcgcgacc cggaggcggt
      181 gcgccggctg cgctggctga cgggcggcga ggcgcggcac ccaaccgtct acattggcgg
      241 cgaatggctg gtcgagccga cgaccagcga gctgggctgg gcgctgacgc ggagcgggct
      301 ccgctagcca tgagctgcat tccgccctca cccgtgaccg acctccaccc ccggcccctc
      361 tctctgcgcg ctgcttgcgg cgaccatgct cgacaatgcg gtcggccgcg ttgccttgtc
      421 ctagtgcctc tgctagggtg gcgcaccgga taccgggcgg gtgcgaggga tggggatgca
      481 gactcacagt ggacgaccgg cggcggcgcg gccagcgctc atcatgatgg cgctcggcgt
      541 gctggtcgcc gcgagcatgc tcgacggggg cagcgggcaa tcgctggcgc aggagggcgc
      601 ggccacgccc gcgacgccgg tgctggccac gccgctggcg atcgatttca cccaaccggt
      661 tgcggagcgg ccagccgcga tcatgtctgg gtcgtgcgac gatcccggcg aggtgatcgc
      721 gccgctgacg gcgctggagc accccgaggg cgaggcgcag gggcagggcg cggcaatcga
      781 agccgcgcgc tcgtatacga gcttacccat tgatctcact tcgctgctgg aggggggagc
      841 gagcgtcaat atcctcttct ccgacgagga accggagacg gtcatcgcct gcggcgagat
      901 cggcgggacg cagggggagt ccgggtcgat tgtgatcaag ctctcgccgc ggaacggatc
      961 gggctacagc gggatcgcct tcctcctgcc cggcgacgac ggcacgacgg gcatctcggt
     1021 cttcgtggcc ggggagcaga ccgtcgccga cacgcacgag gtggcggcgg tggcatctcc
     1081 tggcccaggg ctgatcccac tggagccagc agaagcgaca gccgtaccga caccgacggc
     1141 ggagccagtg caggtggtcg acgtggcgct cctggagtgg ctgatcgaca tgccggcgga
     1201 gatccgggct gggcagatca atttcgcgat cacgaacgag gggaccgagg cgcatagctt
     1261 cacgatcgaa agcggcggcg tggtggtcgc ggagctggac caaccaatcg atccgggcga
     1321 ttcgaccgtg ctgtccgtga cgctggcgga gggggagtac gatgtctact gcccgctggg
     1381 agacggcgag caccgcgaaa aggggatgga gacgacgctg acggtgacgc cgtagtgatg
     1441 atgagcggcg gtcgagccat gggagatcgc tgctcgcgca tgaggggggg actttggcgc
     1501 gaatggagcc gatccggcgc cgggctgcca cgtgagggga catcgctttc gggtggctcg
     1561 cgttccgctg cgccagtttc ctgctgctcg ggatcgttat gcccgactgg ttcgcccgca
     1621 tgtcgtggga aagcaggaac aagaaccagc tcacggtcat cagcgattcg cccgcgagct
     1681 acgtcggtct gctgagcctg tgcgggatcg ttgcctgcgt cgccctcgcc ctcgggctat
     1741 ggcagcggcg gtgggtcgtc gccgcgatgc tcgtcgccgc cggtgccttt gcctacgcgg
     1801 cgtatgtcag cgggagctac tggctgggtc tctcgcgagg ggtgatgctg cttgacggcc
     1861 ggctagtcca ggacatgggt tcggagatga cggcccactg gcctcgcgcg ctgcctctgt
     1921 tcgtgatcgc tgccatcgcc ggagcgatca gcgcactggc tctcacggtc tactggcgag
     1981 aggccttgcg tgcaacgcga tgttggggag caaggcggga agctaactgt cactcacgtg
     2041 gtgggaagag gtctacgcca aagatgtccg ccagaatctg ataagctacc acttgacggc
     2101 tcatgccact ggccgttgtt cctccagaaa cactcacccg aacgaggatg ttacttggta
     2161 aaggactccc gttctggata ccagtgtctg gatctcggcg cgcatacagc gtgtattcgg
     2221 taccgttgta aagcgcatag atgaccacat cgtcgagtgt ctcaatgttg accttgccta
     2281 ggcccgcatc attcagttcc tcttccagtt gcgctctcgt ctttacctcc tgttgcaaat
     2341 gcaagtcgtt cgcgcgttgg tagccaaacc acctctgggc tagagtcgct ccttctgatc
     2401 cggaaaagtc atgaatgctc acatcgatgg tcaattgttc gcgagcattg aaatagacgc
     2461 gttcagcctg accaaaccag cagtaggcat ttaggcgcga cagagcatcg gcggggtcag
     2521 agaacgtcgc cgccaattga tcggcatcgc gacgctcatc gctcacgatc acgtagttgc
     2581 cccacaggtg gggaggtagt agtcaacggg atacgctacg ccgcttgcca gacagccctc
     2641 cagtccctga gaagctgcgg aggagatcgg catgctgaac gcggttgcgg tgacaaggac
     2701 gacagcgatg catcgaagga ggatgaccgt tcccatggct attcctttag acacagacac
     2761 gaggtcaagt tgccaatgtc cgattctagc agccgcttgc ccccgtccac ccgcggatta
     2821 ctctcgctat ccgcgctatc ccgtacaaac cgcgcttcgc tctcgctgaa tcgacggtgc
     2881 tcctgatgcc agcacctgat gagatgtcaa cgaaatatcg cttcgtgatt ccatccgaag
     2941 tcgccgtttc cccgcgttct ccaccggtga ttgttcggtc aggaggaacg tcgcgtgctc
     3001 acccgccgcc gaccgcgtcc ccctcattca gctcaaacgc cagcatggcg gcgttgacaa
     3061 tctcatcagg gtcgatgccg gcttatccat agagggcggc gcgggagccg gactggctcc
     3121 atttgtccat acctaggggc ggttgaggga attcatttca tcagagcatc tgagtaccat
     3181 ttccagtgta cgcgaaggga gacatcatgg ccgattccag accagcaacg aggaccgtga
     3241 cggtcgcgga catcgagggc caactcgccc cgcttctccg agacatcgac cgtggtgagc
     3301 ttcgggtact tgtcgagcag gagggccttc cgatcgcagc catcatctcg gtaagagatc
     3361 tggagcgttt cgatcggctc gagcgagagc gagacgaacg ctttgcggtc atcgatcgga
     3421 tgcgggaggc gtttgcggac gtcccgatcg aagagatcga gcgagaggcg gagcggtccg
     3481 tcgccgaggc gagggatcgc ctgcgacggc gggcagacga gctcactgcc cgctccgcgt
     3541 gatcgttgcg gtcctggata ccaacgtgct ggtctcgggg ttccccgcgc ctacaggcgt
     3601 cccgggcacg ctgatcgatt tgtggcggcg gggcgtgtat cggctcgtcg tctctgagcc
     3661 gatcctcatg gaactggccg aaacgtggcg cgacccatac tgggcggccc gttttccctc
     3721 ggacgagagc aaagcggcca tcgatttgct gcgcgacatg gctttggtca cgccgctgac
     3781 ggtcgaggtc gccggtgtgg ccacgcatcc cgaggatgac ctcatcctgg cgaccgcggt
     3841 ctcgggcggt gctgactacc ttgttaccgg cgaccgaaag gtgctggcca tcggcgcctt
     3901 tgggaacgtc accatcttct cgccacgcgc cttcctaacg cgtttggcgg aagatgcctg
     3961 acggctctcg ggcgcagccc ctcctgcgcg gcgccctcac ccctcctcac tcagctccag
     4021 cgctaacatc gcggcgttga cgatttcgtc ggcgtcgatc cctgcgtact catacaacgc
     4081 ggcgcgggag ccggattggc cccatgtgtc catgccgagg ggaacggtgg gc
//
LOCUS       DASSGX010000202         3125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_39760, whole genome shotgun sequence.
ACCESSION   DASSGX010000202 DASSGX010000000
VERSION     DASSGX010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39760"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VFI12_03555"
     CDS             complement(<1..293)
                     /locus_tag="VFI12_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055510.1"
                     /translation="MISIRQGTIDDFDAILMLMDEAVAWLAGQGRTGQWGSEPFSTLT
                     RSVEFMRGEIEENDLWLAEIDGEPVGAMLLGEAPTRYVESVAERELYLHLLVTS"
     gene            complement(290..787)
                     /locus_tag="VFI12_03560"
     CDS             complement(290..787)
                     /locus_tag="VFI12_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055511.1"
                     /translation="MRDFNQASLDEHLANLAQTLTPEQAEQVTEKVRQRASGGWFDLT
                     AASIDGAFELVGAVLNGAVSLPGNLGGVGDRLAGLFGALDPRQLGGIGNLLGSVPDLG
                     GAAAGAIGELAGHAGDAAGMVAELAGNADAVTDILGSVLDAAPSAGELAGFILEAIGE
                     VLSNP"
     gene            908..1915
                     /locus_tag="VFI12_03565"
     CDS             908..1915
                     /locus_tag="VFI12_03565"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HET7055512.1"
                     /translation="MKIGFASLIGMDPMPFPDLVAWAAANTFDAIEVNVGANFARIGD
                     ASFPGHLDLAGIARNGPGDVPELLARHRIEIASLAPMVNLLTADLALREVRIAEFRAV
                     LDVAAALGVSTVVTFTGSAHGMHFWGLPGVGDGHSSNHVADNLRIFRDVYEPLADYAG
                     ERGIRIAFETAGRGGPEGNLAHSPELWDAMFDAVPSPALGLSFDPSHLVWLQIPNIPD
                     VIRAYGSKIIHFDGKDTEILPARLARQGILGNGWWRYRLPGLGELDWSAIFSALADIG
                     YDGVIAIENEDPLCPGAEGVRWAADYLRAHLLPVQREPGGASGWTDLLEPLKRESENA
                     N"
     gene            1905..2966
                     /locus_tag="VFI12_03570"
     CDS             1905..2966
                     /locus_tag="VFI12_03570"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET7055513.1"
                     /translation="MPTDQVRVALVGCGKIAAIHAKALDALPEADFVACCDREINRAA
                     GLATQYGVPHVSGDVVALFRSGLVDAALVCTPHPAHEAVVVAAAEAGVHVLCEKPIAT
                     TLGEADRMIAAADRAGIRFGVIFQRRFWPSVQRMRAAIDAGKIGVPVLGECAVRLWRG
                     PDYFAMDPWRGRWATEGGGVLMNQAIHSVDHFQWFMGRAIEVSGRYETLRHGGYIDVE
                     DTAVATVRFESGALGVLQASSTFQPAYGFRVAVHGDSGATISVWERNEGELGVNDVWS
                     IPGEEGLREQWLAEDTGKEGFPDYHILQIQEFLQSVLADREPAVTGAEARKSLEIILA
                     IYESSRTGLPVTLPMAAPH"
BASE COUNT          505 a         1103 c          996 g          521 t
ORIGIN      
        1 gatgtcacca gcagatgcag gtacagctcc cgctccgcaa ccgactccac gtaccgggtc
       61 ggcgcctctc cgagcagcat cgcgccgacc ggctcgccgt cgatctcggc gagccagagg
      121 tcgttctcct cgatctcgcc acgcatgaac tccaccgagc gggtgagcgt cgaaaacggc
      181 tccgatcccc attgcccggt ccgcccctgc cccgcgagcc aggcgaccgc ctcgtccatc
      241 agcatgagga tcgcgtcaaa gtcgtcgatc gtgccctgtc gtatcgagat cacgggttgg
      301 acaggacctc gccgattgcc tcgaggatga agccggccag ttcgccagca ctcggcgcgg
      361 cgtccagcac actcccgagg atatccgtga cggcatcggc attgcctgcc agctccgcca
      421 ccatcccagc ggcatccccg gcatgccccg ccagctcgcc gatcgcgccg gcagcggctc
      481 cgcccagatc cggcacactg ccgagcaggt tgccgatccc gccgagctgg cgcggatcga
      541 gcgcgccgaa cagtcccgcc agccgatccc caactccacc gagattgccc ggcaacgaga
      601 ccgccccgtt cagcaccgcc ccaaccaact cgaaggcgcc gtcgatcgat gccgcggtca
      661 gatcgaacca tccgcctgag gccctctgcc gcaccttctc cgtgacctgc tccgcctgct
      721 ccggcgtcag ggtctgcgca aggttggcca aatgctcatc gaggctggcc tgattgaaat
      781 ctcgcatcgt cgttcttcct gtcctgcagg cgattcgccg ggatcatgat cgcggcgagc
      841 gatccattcg tcgattggct atgctgtcgc cgatcgggaa gcgtatcgca gacaggcgga
      901 agcggcagtg aagatcgggt ttgcctcgct catcggcatg gaccccatgc cattccccga
      961 cctcgtcgcc tgggccgccg ccaacacgtt cgacgcgatc gaggtcaacg tcggcgcgaa
     1021 ctttgcccgt atcggcgacg cctcttttcc cggccacctc gatctcgctg gtatcgcgcg
     1081 gaacggtcca ggcgacgtgc ctgagctgct ggcccggcac cggatcgaga ttgcctcgct
     1141 ggcgccaatg gtcaacctgc tgaccgccga cctggccctg cgtgaggtgc ggatcgcgga
     1201 gttccgcgcg gtgctcgacg tggcggcggc gctcggcgtc tccacggtcg tcactttcac
     1261 cggttcggcc cacggcatgc acttctgggg cttgccaggc gtcggcgacg gccactcgag
     1321 caaccacgtc gccgacaatc tgcgcatctt ccgggacgtc tacgagccgc tggccgacta
     1381 tgccggggag cgcggaatcc ggatcgcctt cgagacggcc ggacgcggtg ggccagaagg
     1441 caacctcgcc cacagccccg agctctggga tgccatgttc gacgccgtgc cgtcgcccgc
     1501 actcggcctc agcttcgacc cctcgcacct cgtctggctc caaatcccga acatcccgga
     1561 cgtcatccgg gcgtatggat cgaagatcat ccatttcgac ggcaaggaca ccgaaattct
     1621 gccggcccgc cttgcgcggc agggcatcct tggcaatggc tggtggcggt accgcctgcc
     1681 cggactcggc gaactcgatt ggtcagcgat cttctcggcc ctggccgaca tcggctacga
     1741 cggcgtcatc gccatcgaga acgaggatcc cctctgcccg ggcgcggagg gagttcgctg
     1801 ggcagccgat tatctccgcg cgcacctgct cccggtgcag cgcgaaccgg gcggagcatc
     1861 cggctggacc gatcttctcg agccactgaa acgggaatca gagaatgcca actgatcagg
     1921 ttcgtgtggc gctcgtcggc tgcggcaaga tcgcggcaat tcacgccaaa gccctcgacg
     1981 cgctcccgga agccgatttc gtcgcctgct gcgaccggga gatcaaccgc gcggcggggc
     2041 ttgccaccca atacggcgtc ccccacgtgt cgggcgacgt ggtcgcgctg ttccgcagcg
     2101 gcctggtcga tgcggcgctg gtctgcaccc cacatccggc ccatgaggcg gttgtcgtgg
     2161 cggcggccga ggctggggtg catgtcctgt gcgaaaagcc gatcgcgacg acactcggcg
     2221 aggcggatcg gatgatcgcg gccgcggacc gggccggcat cagattcggc gtcatcttcc
     2281 agcgccggtt ctggccgtcg gtccagcgca tgcgggccgc gatcgacgcc ggcaagatcg
     2341 gcgtcccggt tcttggcgag tgcgccgtcc gtctctggcg cggtcctgac tacttcgcca
     2401 tggatccctg gcgcggcagg tgggcaacgg aaggcggcgg cgtcctcatg aaccaggcga
     2461 tccactcggt cgatcatttc cagtggttca tgggccgcgc gatcgaggtc agcggacgct
     2521 acgagacgct tcgccacggc gggtacatcg acgtcgagga tacggccgtg gccacggtcc
     2581 ggttcgagag cggcgcgctc ggcgtcctgc aggccagctc cacgtttcag ccggcatacg
     2641 ggttccgggt cgccgtgcac ggcgattccg gcgcgacgat cagtgtctgg gagcgcaatg
     2701 agggcgaact cggggtcaat gatgtctgga gcatccccgg cgaagagggg ctgcgggaac
     2761 agtggctggc ggaagacact gggaaggagg gcttccccga ctaccacatc ctccagatcc
     2821 aggagttcct gcaatcggtc ctggccgacc gcgagccggc agtcaccgga gccgaggccc
     2881 gcaagtcgct cgagatcatc ctcgccatct acgaatccag ccgcaccggc ctccccgtca
     2941 ccctgccaat ggccgcacca cactgattcc cggataacga acgggttgcg cgacgctgca
     3001 ggcgtttttt cggagatgta gcgcggggcg gctcgccccg ctgtggccat aggccacgac
     3061 ggtctctccg aaaagccgtc cccgcacgga tgccagttgc tcggaagcca atccggggcg
     3121 agtcg
//
LOCUS       DASSGX010000203         2347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1455032, whole genome shotgun sequence.
ACCESSION   DASSGX010000203 DASSGX010000000
VERSION     DASSGX010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455032"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="VFI12_03575"
     CDS             <1..682
                     /locus_tag="VFI12_03575"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055514.1"
                     /translation="ADWALLLTALWHGLLGARISVNEYVRPHGWRTVINAVLWVVGFF
                     FTILGTVTILTFDEAAARNNTGPLAGSFWIGDILGYSLYVLAAITYLAVILLIVAAFR
                     QKGREGRPFFYMGDPGQWAWILHRATGLGIVGFLLVHILDILLIGLGRDVYDHTVEFY
                     GRLFIVPMEIALVGAVLYHGLNGLRITILNFWDVGVEKERPLFYAALVGAVLLTLPSL
                     IVILQHEL"
     gene            799..2187
                     /locus_tag="VFI12_03580"
     CDS             799..2187
                     /locus_tag="VFI12_03580"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HET7055515.1"
                     /translation="MRAIPRLVASLICAVAVGLALSLIAPATDAQSDQPLVLVAKIDG
                     PITPVMARYIGRAVSKAEDRNAAALVLEMNTPGGLSTAMDDIIDDIVQSTVPVIVYVS
                     PVNARAASAGVYITYASHIAVMAPSTNIGSASPVMTGTSGNVTTDETMERKVMNDAVA
                     RITNLAQLRGRNVDWAVSAVRDAANITSQQALDLHVIDLIEPNLDQLLADVNGRTVNL
                     NSGPVTLNTANAKVENVDMGLIEQFLQMLADSTLAYLLVSFGMLGLFIELSHPGIGVP
                     GIFGGIALILGLLGLGSLPVVWAGLAFMVLAFVLFVLDLFVPSAGLLTVGGLISFLIG
                     SNILFAGSAPGSLRASKAVIYTMTACLGVSAVLLGMAVVRARFRKTKTGKAGMIGQIG
                     VARTDLAPDGMVLVFGELWAATAEGPPIEEGQNVYVLAVSGLSLRVRRATVEEVIALT
                     DVVDRRAVVPVR"
BASE COUNT          358 a          801 c          720 g          468 t
ORIGIN      
        1 tgccgactgg gccttgctcc tgaccgcact ctggcacggg ctgctcggcg cgcggatctc
       61 ggtcaacgag tacgttcgcc cgcacggctg gcgcacggtc atcaacgccg tcctctgggt
      121 ggtcggcttc ttcttcacca ttctcggcac tgtcacgatc ctgacgttcg acgaagcagc
      181 cgcccgcaac aacaccggcc cgctcgccgg ctccttctgg atcggcgaca tcctcggtta
      241 ctcgctttat gtccttgccg cgatcaccta cctcgcggtc atcctgctga tcgtggccgc
      301 cttccggcag aagggccgtg agggtcgccc cttcttctac atgggcgacc ccgggcaatg
      361 ggcctggatc ctgcaccgcg cgaccggcct cggcatcgtt ggcttcctgc tggtccacat
      421 cctcgacatc ctgctgatcg gcctcggccg ggatgtctac gaccacacgg tcgagttcta
      481 cggccgcttg ttcatcgtcc cgatggagat cgcgctggtc ggcgctgtcc tctaccacgg
      541 attgaacggc ctgcggataa cgatcctcaa tttctgggat gtcggcgtcg agaaggagcg
      601 cccgctcttc tacgccgccc tcgtcggcgc ggtcctgcta acgctcccta gcctgatcgt
      661 catcctccag cacgagctgt agcctgcagc cgggcgaact cgcccggcac tacatcacat
      721 cgtgggtttc cggacgtgtc attcctttag aaccgctgtt ccatatctcg tatagtagag
      781 gctgaaggag gtgacgccgt gcgcgccatc ccacgcctcg ttgcttcgct gatctgcgcc
      841 gtcgcagtcg ggctcgccct gagcttgatc gctcccgcga ccgatgcgca atccgaccag
      901 ccccttgtcc tcgtcgcgaa gatcgacggc ccgatcaccc cggttatggc ccgctacatc
      961 ggccgcgctg tttcgaaggc ggaggaccgc aacgccgccg cgctcgtgct cgagatgaac
     1021 acgcccggcg gcctctcgac ggcgatggac gacatcatcg acgatatcgt ccagagcacg
     1081 gtcccggtca tcgtctacgt ctcgccggtc aacgcccgcg ccgcctcggc cggggtctat
     1141 atcacctacg cctcgcacat cgcggtcatg gcgccctcga ccaacatcgg ctcggcctcg
     1201 cccgtcatga ccggcaccag cggcaatgtc accaccgacg agacgatgga gcggaaggtg
     1261 atgaacgacg ccgtcgcgcg catcaccaac cttgcccagc tccgcggccg caacgtcgac
     1321 tgggcggtct ccgctgtcag ggacgcggcc aacatcacct cgcagcaagc gctcgacctg
     1381 cacgtgatcg acttgatcga gccgaacctc gatcagctcc tggccgacgt caacggccgc
     1441 acagtgaacc tcaactccgg cccggtcacg ctcaacacgg caaacgccaa ggtcgagaac
     1501 gtcgacatgg ggctgatcga gcagttcctg cagatgctgg ccgactccac gctcgcctac
     1561 ctgctggtca gcttcggaat gctcggtctc ttcatcgagc tttcgcaccc aggcatcggt
     1621 gtgccgggca ttttcggcgg catcgccctg atactcggcc tgctcgggct cggcagcctg
     1681 ccggtcgtct gggcgggcct cgccttcatg gtcctggcct tcgtgctgtt cgtgcttgac
     1741 ctcttcgtgc catcggccgg gttattgacg gtcggagggc tgatcagctt cctgatcggg
     1801 tcgaatatcc tcttcgcggg cagcgccccg ggcagtttga gagcgtcgaa ggccgtcatt
     1861 tacaccatga ctgcctgcct cggcgtctcc gcggtgttgc tcggcatggc cgtggtccgc
     1921 gctcgcttcc ggaagacgaa gacgggcaag gccggcatga tcgggcagat cggcgtcgcc
     1981 cggacggatc tcgcgccaga cggtatggtt ctcgtattcg gagagctctg ggcggcaacc
     2041 gcggaagggc cgccgatcga ggaaggccag aacgtctacg tcctggccgt gagtggcctg
     2101 tcgctcaggg tgcggcgggc gacggttgag gaagtgatcg cattaaccga cgtcgtcgat
     2161 cgacgtgcgg tcgtgcccgt gcgctagcgc cgggtgtcca tagaggagta cgcagttgac
     2221 tgcattggtc gtattgggcg tcatcgtcct gttcctcatc atcgtgctgt tgagcgcgat
     2281 caaggtggtg caagagtatg agcggggcgt cgtcttccgg ctcggccgga tcgccggggt
     2341 ccgcggg
//
LOCUS       DASSGX010000204         3071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_148172, whole genome shotgun sequence.
ACCESSION   DASSGX010000204 DASSGX010000000
VERSION     DASSGX010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148172"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1066
                     /gene="dnaB"
                     /locus_tag="VFI12_03585"
     CDS             <1..1066
                     /gene="dnaB"
                     /locus_tag="VFI12_03585"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HET7055516.1"
                     /translation="SLVSVVPTAVHVEYYARIVERTATLRRLIDAGTRVVGIAFRDAI
                     ETQEALDEAEQAIFEVSQNRVPRGFLPMREVLDGLFDQMNEDGLRVRGVPTGFVALDR
                     VLGGLQRSDLIILAARPSVGKTALALSMAYNAAVQHRKVVGFISLEMSAQQIVQRVLA
                     SETGVNSHRLRMGQINDDEWGAVGRAYGKLSEAPIFIDDGGSSTVNDVRTKARRLQAE
                     FGLDMLIIDYLQLMQSRRSDNRVQEISEISRGLKQVARELDIPVVALSQLSRAVESRA
                     GHKPLLSDLRESGSIEQDADVVMLIYREDRYDPETSRKGIADIDIAKHRNGSTATVSL
                     RFFEATTRFADFEPYREEPV"
     gene            1080..1736
                     /locus_tag="VFI12_03590"
     CDS             1080..1736
                     /locus_tag="VFI12_03590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HET7055517.1"
                     /translation="MTDLPPAAPVDSAPAVSVPRALLDRLITSRSSVEEHRIVLLLVL
                     LTRTGKADAAISEDAFVSDPAVIDAGKVDGTPIAMPDWPYPAIEQAVAHGTILRFVVE
                     SPGGSRSWLLLNTVQNARLVSTMADNPDTVPEIYWIDESRPRVHVDRPTVFRLYEQNI
                     GPLTPLIADRLIKALELYPAEWVESALEEAVSYNRRNWRYIARILENWAADGPPSRTN
                     "
     gene            1748..2539
                     /locus_tag="VFI12_03595"
     CDS             1748..2539
                     /locus_tag="VFI12_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7055518.1"
                     /translation="MSDIPFNISRLSGSDTEETVYRPADQDICPICKGAGWLRLDVPL
                     GHPSFGRLFKCECTQRSAERARANEMRRLSQLDGLESKSFETFEAGTPDLARALEVAK
                     SFVQKLSGWLVLTGPCGTGKTHLAAAIANRILDAGDQTVMFAVVPDLLDHLRATFEPS
                     QGIAYDQRFNDIRNTYLLVLDDIGTENATPWAREKLYQIINHRYNERLPTVITSNQPD
                     SAIDERILSRMLDRDLSQRLQMIGEDYRRRGDPTYLRGRRRRVSA"
     gene            2633..>3071
                     /locus_tag="VFI12_03600"
     CDS             2633..>3071
                     /locus_tag="VFI12_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055519.1"
                     /translation="MSQDITTSRAGERTVGAQTEQSDIIKRRLGAWTAPMAAQTAPMP
                     DRPDMGGATLNLGNGAPAVTAPIMGSDATMTSRLHQVTGAIPSNGGTGATRPMAEPQT
                     VPFAASPAPAGTVPFPANGQTAQGLHPSGAMTAAMASRADNTVP"
BASE COUNT          567 a         1018 c          946 g          540 t
ORIGIN      
        1 atcgctggtc tctgtcgtgc ccaccgccgt gcacgtcgag tattacgcgc gcatcgtcga
       61 gcgaacggca acgcttcggc gtttgataga cgccggcacc agggtcgtcg gcatcgcctt
      121 ccgcgacgcc atcgaaaccc aggaagcgct ggacgaggcc gagcaggcga tcttcgaagt
      181 ctcgcagaac cgggtaccgc gcggcttcct gccgatgcga gaggtgctcg acggcctctt
      241 cgaccagatg aacgaggacg ggctccgggt tcgcggtgtc ccgaccggct tcgtcgcgtt
      301 ggaccgggtt ctgggcggtc tccagcgatc cgacctgatc atactcgcgg cccggccgag
      361 cgtcggcaag accgcgctgg ccctctccat ggcctataac gccgccgttc agcatcggaa
      421 ggtggtcggc ttcatttcgc tggaaatgtc agcgcagcag atcgttcagc gcgttttggc
      481 ctcggagacc ggggtcaact cgcaccgcct gcgcatgggg caaatcaacg acgacgaatg
      541 gggcgcggtc ggccgcgcct acggcaagtt gagcgaggcg ccgatcttta tcgatgacgg
      601 cggcagttcc accgtgaacg acgtccgcac gaaggcccgt cgcttgcagg ccgagttcgg
      661 gctggacatg ctcatcatcg actacctgca actcatgcag tcccgccgct ccgacaatcg
      721 cgtgcaggag atctcggaga tctcgcgggg cctcaagcag gtcgctcgcg aactggatat
      781 cccagtcgtg gcgctttctc agctctcccg cgcagtcgag agtcgcgccg ggcacaaacc
      841 gctgctgtcg gacctccgtg aatcgggctc aatcgagcag gacgcggatg tcgtcatgct
      901 gatttaccgc gaagatcgct acgatccgga gacgtcgcgg aagggcattg ccgacatcga
      961 cattgccaag caccgaaacg gcagcaccgc gaccgtgtca ttgcgcttct tcgaggcgac
     1021 gacccggttc gccgatttcg agccgtatcg cgaagagccg gtctaggcac gatcaagtga
     1081 tgactgacct gcctcccgcc gcgccggttg attccgctcc cgctgtctcg gttccgcgtg
     1141 cactgctgga tcgattgatc acgtcgcgct cgtccgtcga ggaacacagg atcgtcctcc
     1201 tcctggtctt gctcactcga accggcaaag ccgatgccgc catctccgaa gacgcgttcg
     1261 tatctgatcc cgccgtgatc gacgcgggta aagtcgacgg cacgccgatt gccatgccgg
     1321 actggccgta tccggcgatc gagcaagccg tggcgcacgg tacgatcctg cggttcgtgg
     1381 tcgagagccc cggcggctcc cggagctggt tgctgctgaa tacggtccag aatgcacggc
     1441 tcgtgtccac catggctgat aacccggata ccgtgcccga gatctattgg atcgacgaat
     1501 cgcggccgcg cgtgcatgtc gatcgcccga ccgtgttccg tctctacgag cagaacatcg
     1561 gacccctgac gccgcttatt gccgatcgac tcatcaaggc actggagctc taccccgcag
     1621 agtgggttga atctgcactg gaagaggcgg tttcgtacaa tcgtcgtaac tggcggtata
     1681 tcgctcgcat cctcgagaac tgggctgcgg acggtccgcc gagcaggacg aactgatgat
     1741 gcgacccttg agtgacattc ctttcaatat ctcgcgactg tctggctccg acaccgaaga
     1801 gactgtgtat cggccagcgg accaggacat ctgcccaatt tgcaaggggg cggggtggct
     1861 tcggctcgac gtgccacttg gacacccgag cttcggccgg ttgttcaagt gcgaatgcac
     1921 gcagcgttcg gccgagcggg ctcgcgcgaa cgagatgcgg cgcttgtcgc aactcgacgg
     1981 gctcgagtcc aaatcattcg agacgttcga ggccggcacc ccagacttgg cacgcgcgct
     2041 tgaggtcgcg aaatccttcg tccagaagct gagtggttgg ctcgtgctga ccggtccgtg
     2101 cgggaccggc aagacgcacc tcgccgccgc aatcgccaac cgcatcctgg atgcaggcga
     2161 tcagacggtc atgttcgccg tcgtcccgga cctgctcgac cacctgcggg cgacattcga
     2221 gccgtcccag ggcatcgcct acgatcagcg attcaacgac atccggaaca cctacctgct
     2281 cgtgctggac gatatcggga ccgaaaacgc aaccccgtgg gcgcgcgaaa agctctacca
     2341 gatcatcaat caccgctata acgagcggct cccaaccgtg attacgagca accagccgga
     2401 ctccgccatc gatgagcgga tcctctcgcg aatgctcgac cgggacctct cccagcggct
     2461 gcaaatgatc ggcgaggact accgacgtcg cggtgatccg acctacctgc gcggccgccg
     2521 gcggcgggtc agtgcctaag tcctattctc cggctaggcc cttcccccta gactcgaatc
     2581 agccgaattt ccgcctggtg cggatgggag tggctgtgca cggagggaat cggtgagcca
     2641 ggacatcacc acgtctcgcg ctggcgaacg aacagtcggg gcccagacgg aacaatcaga
     2701 catcatcaag cgacggctgg gtgcctggac tgcgccgatg gcggcgcaga ccgcgcccat
     2761 gcccgaccgt ccggacatgg gcggcgccac gctcaatctc ggcaacggcg ctccggctgt
     2821 aacggcaccg atcatgggca gcgacgccac gatgacgtcc aggttgcatc aggtaaccgg
     2881 cgccatcccg tcgaacggcg ggaccggggc aacccggccg atggccgaac cccagaccgt
     2941 gcccttcgcc gcatctcctg caccggccgg gaccgtaccg ttcccggcca atggccagac
     3001 cgcccaaggt ctgcacccgt ccggcgcaat gaccgctgcg atggcttctc gggcagacaa
     3061 taccgtgccg c
//
LOCUS       DASSGX010000205         4119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1237091, whole genome shotgun sequence.
ACCESSION   DASSGX010000205 DASSGX010000000
VERSION     DASSGX010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237091"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1291
                     /locus_tag="VFI12_03605"
     CDS             <1..1291
                     /locus_tag="VFI12_03605"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7055520.1"
                     /translation="EQRRWSTRNRIRAIPLAAAAIAAPTILRHSTLAQQAEIRLTGWT
                     SSDQEQKLFEQILADFGTAHPDIKLKYEPIPSDYATKLQTDIAAGSVADVFYVDSMAA
                     PDFMVNGALLELDSMMAKDGVKAEDFYPGLIQAFQMGGKTYGLPKDWSSLAMVYDKQA
                     LTDAGVTTPPTNWDELKAAGQALKDKSGEARINIPPDIAREFAFHYAAGAKVISDDGS
                     QIVIDSPEAATALDFYYGLYKDGLASTPADAGAEWPGDALAKDFADIVFEGNWVFPFL
                     KENAPDLDFGISEMPDGPAGKATLAFTVSFSAYAETKQPDAAWTVINYLTGAEGMGKW
                     TSLGLAMPSRTALADAWLKQFPEREPFLKSGDYAHGWQLGPGGQAFYNDANGDLQGLF
                     AGTLDVQTTLKNWQKEAETRIKLGGGGAPSPEASPMS"
     gene            1373..2476
                     /locus_tag="VFI12_03610"
     CDS             1373..2476
                     /locus_tag="VFI12_03610"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET7055521.1"
                     /translation="MAIAAAPATRTRRRNPIVVEDQRAGWVFLAPAMAVFLVFIFGAI
                     LFAIYLSFHNYALLQKGGIWPVFTHPGRTWVGLDNYRDIMHNGFFWQAFRNTTWYAIG
                     VVPAQTILGLVLAVLANRKIRGRTFFRTAFYFPSISSSVVISIIFLWMYSARGFVNYA
                     LRGLGFPTPRPVWLANPKGVVGMLLDQFGIHSVPNWLEGPSVALLSIMMLNVWTTTGT
                     IMVIFLAGLQGLPGDVYEAANLDGANRFRQFTDITVPLLKPIIGFAVTIGLIGTFQVF
                     DQIYVMSEGGPQKTTTTIAYLIYVEGFQQGRGLGYASAIAIILLFVIMCLYLVQRRFT
                     TERETDERVPGGIRAWVARNLIGQDRGATGGAR"
     gene            2476..3369
                     /locus_tag="VFI12_03615"
     CDS             2476..3369
                     /locus_tag="VFI12_03615"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET7055522.1"
                     /translation="MAVATETLQAKPQATESREVTSTTDRVLNWVAYVVLTIVALSMF
                     IPFIFSVSTSFKTNREATLPLSIQRLFWPDVIVSDAYHKVFDADIQRWFANSVIVAAV
                     WVIARAFTASTAGYAFARMQFKGKNLLFLLVLATMMIPGMVTIVPKFLILRQLGIINT
                     YGALTLPFMADAFGIFLMRQFFESIPVELEEAARVDGASRYRMFAQIILPNSMPALIA
                     LAIFSFQGAWNAFLEPLIFINNPKLYTLPLGLAYFRRANYTEWPTLMAIAVITTVPMA
                     IFYIIFQRWFVEGQVQSGIKG"
BASE COUNT          681 a         1466 c         1276 g          696 t
ORIGIN      
        1 cgagcaacgg cgctggtcga cccggaaccg gatccgcgcg attccgctcg cggccgcggc
       61 aatcgccgcg ccgacgattc tgcggcacag caccctcgcc cagcaggcag agatccgcct
      121 caccggctgg acctcgtccg accaggagca gaagctgttc gagcaaatcc tcgccgactt
      181 tgggaccgcg caccccgaca tcaagctgaa gtacgagccg atcccgtccg actacgcgac
      241 caagctccag accgacatcg ccgccggcag cgtggccgat gtcttctacg tcgacagcat
      301 ggccgcgccg gacttcatgg tcaacggcgc gctgctcgag ctcgacagca tgatggcgaa
      361 ggacggcgtc aaggccgaag acttctaccc cggcctgatt caggcgttcc agatgggcgg
      421 caagacctac ggcctgccga aggactggtc cagcctggcg atggtctatg acaagcaggc
      481 cctcaccgac gccggcgtca ccaccccgcc gacgaactgg gacgagctga aggccgccgg
      541 gcaggcgctc aaggacaaat cgggcgaggc gcggatcaac atcccgccgg acatcgcccg
      601 cgagttcgcc ttccattacg cggccggcgc caaggtcatc agcgatgacg gctcgcagat
      661 cgtgatcgat tcgccggaag ccgccaccgc gctcgacttc tactacggac tgtacaagga
      721 cgggctggcc tccacgcccg ccgatgccgg cgccgaatgg ccgggcgacg ccctcgccaa
      781 ggacttcgcc gacatcgtct tcgagggcaa ctgggtcttc ccgttcctca aggagaacgc
      841 gccggatctc gacttcggca tctccgagat gcccgatggc ccggccggca aggcgaccct
      901 cgccttcacc gtctccttct ccgcctacgc cgagaccaag cagcccgacg ccgcctggac
      961 ggtcatcaac tacctgaccg gcgccgaggg catgggtaag tggacctcgc tcggtctcgc
     1021 catgccgtcg cggaccgcat tggctgatgc gtggctgaag caattcccgg agcgcgagcc
     1081 gttcctgaag agcggcgatt acgcgcacgg ctggcagctc ggccccggtg gccaggcctt
     1141 ctacaacgac gccaacggcg acttgcaggg cctcttcgcc ggcacgctgg acgtccagac
     1201 cacgctcaag aactggcaga aggaagccga gacgcggatc aagctcggcg gcggcggcgc
     1261 tccgtcgccc gaagcctcac cgatgagcta acgcccacgc gacccggggc gccgaccacc
     1321 ggcgctccgg gcccattccc gcgagctgac gccgccgtca gaaggggacg ccatggccat
     1381 cgccgccgca cctgcgacca ggaccaggag acgcaacccg atcgtcgtcg aagaccagcg
     1441 cgcgggctgg gtctttcttg cgcctgccat ggcggtcttc ctcgtcttca tcttcggcgc
     1501 gatcctcttc gcgatctacc tgagcttcca caattacgcc ttgctccaga agggcggtat
     1561 ctggcccgtc ttcacgcacc ccggccggac gtgggttggg ctcgataact accgcgacat
     1621 catgcacaac ggcttcttct ggcaagcgtt ccggaacacg acctggtacg caatcggcgt
     1681 cgtgccggcc cagaccatcc tcggccttgt gctggccgtg ctcgccaatc gcaaaatccg
     1741 cggccggacc ttcttccgga ccgcgtttta cttcccctcg atcagctcct ctgtcgtgat
     1801 ctcgatcatc ttcctgtgga tgtattccgc gcgcggcttc gtcaactacg cgctgcgcgg
     1861 gctcggcttc ccaacgcccc gaccggtgtg gctggcaaac ccgaagggcg tcgtgggaat
     1921 gctcctcgac cagttcggca ttcacagcgt gccgaactgg ctcgaggggc cgagcgtggc
     1981 cctgctctcg atcatgatgc tcaacgtctg gacgacaacc ggcacgatca tggtcatctt
     2041 cctcgccggg ctccaggggc tgccgggcga tgtctatgag gcagccaatc tggacggggc
     2101 gaatcgcttc cgccagttca cggacatcac cgtccccctg ctcaagccga ttatcggctt
     2161 cgcagtcacg atcgggctga tcggcacgtt tcaggtcttc gaccagatct acgtcatgag
     2221 cgagggcgga ccgcagaaga cgacgaccac gatcgcctac ctgatctatg tcgaaggctt
     2281 ccagcaaggg cgcggtctcg gttatgccag cgcgatcgcg atcatcctgc tgtttgtgat
     2341 catgtgcctc tacctggttc agcgccgctt cacgaccgag cgggagaccg acgagcgtgt
     2401 cccgggcggg atacgcgcct gggtcgcccg gaacctgatc gggcaagacc ggggcgcgac
     2461 gggaggcgca cggtaatggc ggtcgcgacc gaaacactcc aggccaaacc gcaagcgacc
     2521 gaatcgcgcg aggtcacgtc cacgaccgat cgcgtcctca actgggttgc ctacgtcgtg
     2581 ctgaccatcg tcgcattgtc gatgttcatc ccgttcatct tctcggtctc gacgtcgttc
     2641 aagacgaacc gcgaggcgac gctgcccctc tcgatccagc gcctcttctg gccggacgtg
     2701 atcgtgtccg acgcctacca caaggtcttc gacgccgata tccagcgctg gttcgcgaac
     2761 agcgtcatcg tggcggccgt ctgggtgatc gcccgggcat tcacggccag caccgccggc
     2821 tacgccttcg cgcggatgca gttcaagggc aagaacctgc tcttcctgct ggtgctggca
     2881 acgatgatga tcccggggat ggtcacgatc gtgccgaagt tcctgatcct gcgccagctc
     2941 ggcatcatca acacctacgg cgcgctgacc ctgcccttca tggccgatgc gttcggcatc
     3001 ttcctgatgc ggcagttttt cgaatcgatc ccggtcgagc ttgaggaggc ggcgcgggtc
     3061 gatggggcca gccggtaccg gatgttcgcg cagatcatcc tgcccaactc gatgccggcc
     3121 ctgatcgcgc tcgccatctt cagcttccag ggcgcctgga acgccttcct cgagccgctg
     3181 atcttcatca ataacccgaa gctctacacg ctcccgctcg gcctcgccta cttccggcgg
     3241 gccaactaca ccgagtggcc gacactgatg gcgatcgccg tgatcacgac ggtcccgatg
     3301 gcgatcttct acatcatctt ccagcgctgg ttcgtggaag gccaggtcca aagcgggatc
     3361 aagggctgac gaggcgagca ggcgagcagc aggggcgcgg aggcgcggag gcacgatgat
     3421 ggtaggggtt tgcgggggca aacccgccgc tcctgacgcg cccgttcgca ggcaccggcc
     3481 cccgatgtag gggcagatgg catctgcccg tcctccccaa ccggcgcgtt tgcatgcacc
     3541 ggcggagcgc ggtccccccg tgggccgcat cgatcatccc cacacgcggt tgatgtcgga
     3601 gcgtcaatga tgggggttgg ttccccggaa cgcgcgcgtc gagacggagc ccgggcggcc
     3661 cacgggggga ccgcgctacg gtgcgatcat ccacccgcgc cacgtttggg gacgccgccc
     3721 cccgcagcgg ggggaagcca gatggcggaa gcgccgcggt ggccgcaggc ctcgatggtc
     3781 cgtgccgagg cgcacgttcg cgcgtcggga accgttcggt cgggaacgat catcgccctg
     3841 cgggcgatcg cggggcatgg taaccccgcg ctccgtctgg atgcgggtgc ctgcgaacgg
     3901 gcgcgtcggg aacggcgggt ttgcccccgc aaacccctcc aatcgtcgcg gccccgcacc
     3961 cccgcccccc gccttcgcgc ctccgcgccc ccgcgcccat gcttctcgcc tgctcgcctg
     4021 ctcgcctgct cgcctccgcc tgctcgcctt cgcgcctcat caccccaccg aatactttcc
     4081 gccctgtgag cgctcgtcct cggcgcggcg ctcgggggt
//
LOCUS       DASSGX010000206         2039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1237169, whole genome shotgun sequence.
ACCESSION   DASSGX010000206 DASSGX010000000
VERSION     DASSGX010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237169"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1073
                     /locus_tag="VFI12_03620"
     CDS             <1..1073
                     /locus_tag="VFI12_03620"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET7055523.1"
                     /translation="VATAIQYLSLTFPVDESWTRYNGLQPLVYFITVFIAAPTSIVTG
                     LMQSPAISNRLGWFGRALNRQKARSIHFIALWWFLLFILAHVTLVFITDARVNLNMMW
                     AGVNDASWSGLWIFIAAMIVVALAWWWASPFTLRRARLVQRVGEVMSRPFGAATQSWD
                     AKSQLSEKDISPHFWPNGTMPISAEFDRLVETGFADYRLRVGGLVENAMEFSYAELKA
                     MPKQEQITTHFCIQGWSGVAKWGGVPVRHILDLVKPTADARYAVLYSFADGGDGGRYY
                     DTHSLSNMRHELTILAYEMNGAPVSVLHGAPLRLRCENEVGFKMVKWIAAIELVHDYA
                     DLGAGEGGYNEDHEFFGYRVPI"
     gene            complement(1414..>2039)
                     /locus_tag="VFI12_03625"
     CDS             complement(1414..>2039)
                     /locus_tag="VFI12_03625"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HET7055524.1"
                     /translation="GALTGTYYTATLNPPLPEVVRKINIDEEWVYWSDRVTLPYKPHI
                     GTLSCSPEIDSINSLTPDNHGGNMDLPDMGPGSITYLPVRSPGARLFIGDAHACQGDG
                     EVCGVAVEYPTVTTIHVELIKGWTIEWPRLENDEVIMAIGSARPLEDATRIAYRELVR
                     WMAKDYEFDQWDAYMMLSQCGRVRLGNFVDPKYTVGAAISKKYLAKK"
BASE COUNT          363 a          618 c          634 g          424 t
ORIGIN      
        1 ccgtcgcgac cgcgatccag tacctctcgc tgaccttccc ggtcgatgag agctggacgc
       61 gctacaacgg cctgcagccc ctcgtctact tcatcaccgt cttcatcgcc gcgccgacgt
      121 cgatcgtcac cggtttgatg caaagcccgg cgatctcgaa ccggctcggc tggttcggcc
      181 gcgcgttgaa ccggcagaag gcgcgctcga tccacttcat tgcgctgtgg tggttcctcc
      241 tcttcattct ggcgcacgtg acgctggtgt tcatcaccga cgcgcgggtg aacctgaaca
      301 tgatgtgggc cggagtcaat gacgcttcgt ggagcggtct gtggattttc atcgccgcga
      361 tgatcgtcgt cgcgctggcc tggtggtggg cgtccccttt caccttgcgg cgtgcgcggc
      421 tggtgcaaag ggtcggcgag gtgatgtcgc ggccgttcgg tgctgcgacc cagtcgtggg
      481 acgcgaagag ccagcttagc gaaaaggaca tctcgcccca cttctggccc aacggcacga
      541 tgccgatttc ggcggagttc gaccggttgg tcgagaccgg cttcgccgac taccggctgc
      601 gggtcggcgg actggtcgaa aatgccatgg agttctcgta cgcagaattg aaagcgatgc
      661 cgaagcagga gcagatcacg acgcatttct gcattcaggg gtggtcaggc gttgccaaat
      721 ggggaggcgt gccggtgcgt cacattctcg acctggtgaa gccgacggcg gatgcgcgct
      781 atgcagtcct ctactcattt gccgatggcg gcgacggtgg tcgctactac gacacgcaca
      841 gcttgtccaa catgcggcac gaactgacga tcctggccta cgagatgaac ggagcgccag
      901 tcagcgtgct gcatggcgcg ccgttgcggc ttcgctgcga gaatgaggtc ggcttcaaga
      961 tggtcaaatg gattgcggct atcgagctcg tacatgacta tgccgatctc ggcgccggtg
     1021 aaggcggcta caacgaggat cacgagttct tcggctatcg cgtgccgatc tgattatcga
     1081 aatgagtgaa tgcggatgta ctgaccagac atgagaagcc tgacgactcc aacgaaggat
     1141 cgtccacgct ggaaaggcgt tgaccaaagc tgctttgccg tgcgcgtgcc cgagcagcat
     1201 cgcgccgtcg gtcagtcaac cggtcacctg ccgatggcag cctgacttaa accccgatct
     1261 ctccggaata tccggggcgt cagtttgacc tcccgaggca aaagccggag tgtccctgtg
     1321 tgtccgccgg aatgaactgt tacccttcgc cgcggcaggc tcgcggcagt cggtgcgaag
     1381 ctagggcgag cgttgcccgc aggtgtcgtc gccttacttc ttcgccagat attttttgct
     1441 gatcgccgcg cccacggtgt atttcggatc gacgaagttt ccgagacgaa cccggccgca
     1501 ttggctcagc atcatgtacg cgtcccactg gtcgaattcg tagtccttcg ccatccatcg
     1561 tacgagctcg cggtacgcga tccgcgttgc gtcctccagc ggccgtgcac tgccgatggc
     1621 catgatgacc tcgtcgttct cgagccgcgg ccattcgatc gtccatcctt tgatcagttc
     1681 gacgtggatc gtcgtcacgg tgggatactc aaccgccacg ccgcacactt cgccgtcgcc
     1741 ctggcaggcg tgcgcatccc cgatgaacag acgcgcaccg ggggagcgca caggaagata
     1801 ggtgatactg cccgggccca tatcaggcag gtccatgttc ccgccgtgat tgtcgggcgt
     1861 cagtgaattg atggagtcga tttcgggcga gcagctcaac gtgccgatgt gcggcttgta
     1921 cgggagggtc acgcggtcgc tccaatagac ccactcctcg tcgatattga tcttccgcac
     1981 cacctcgggc aatggaggat tgagcgttgc cgtgtaatag gtgcccgtca acgccccga
//
LOCUS       DASSGX010000207         2677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_692770, whole genome shotgun sequence.
ACCESSION   DASSGX010000207 DASSGX010000000
VERSION     DASSGX010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692770"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="VFI12_03630"
     CDS             <1..514
                     /locus_tag="VFI12_03630"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7055525.1"
                     /translation="QRGFVAEARAELIQRADLEERAGKAKEAIISLQRAADLSWNLGF
                     PQETFNLYDRILAIDPEDVSNRSALVNLYLQVGRLSDAAEHQRAVVDLALKNGRKHEA
                     VAALHQVIGLTPDDTTAYYQLGEALSSMGEFQQAEKVYRRIVLMNPDDAVAQAKATAM
                     AALKEQAQGH"
     gene            611..1522
                     /gene="cdaA"
                     /locus_tag="VFI12_03635"
     CDS             611..1522
                     /gene="cdaA"
                     /locus_tag="VFI12_03635"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HET7055526.1"
                     /translation="MSELTWLLSRLGEPRSIVDIAVVTGLLFWLLWVLQGTRATQLLR
                     GAAIFLIGAILLARTLDLRALGWLIDNMWPAVIVAIPIVFQPELRRALEQLGHTGTWL
                     RTSLPAGSDVTTMSSTIDEVVRAAAQLSRLRYGALMVLERETGLQDYADRGVAIDALV
                     SRQLLVTIFFPNSPLHDAAVLMRGDRIVAGSVVLPLSENISPMSNLGTRHRAGIGVTE
                     DSDALAIIVSEETGQISVAHHGRLIRNLDQDRLRRVLRTLSRVERVDRDTQASGVRRV
                     IAERLGQIAERRGRQRPPGTHPSTSKM"
     gene            1525..>2677
                     /locus_tag="VFI12_03640"
     CDS             1525..>2677
                     /locus_tag="VFI12_03640"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HET7055527.1"
                     /translation="MQQPDERTRKSFATEWGSRLLISLLLAILLWGWVTATGDPETTR
                     QFPSIPISVEGLKEGFVLVGDVGSAEVRVTGPRSAVEDLLSTQIQAFLDLDQISEPGT
                     YTVRIQAPKPNNTWSTAPRPRSMNIVVERETTKAFPVDPKPSGSLGTNQQVETVNPSA
                     SEVTVTGPNSLVERVNQVVLPIDIANRTTDFASVFTPVPIDKSGQPIAGLTLNPSTIS
                     ATVEITTRGKRVAVIAQIEGEPSSGFEVVDRLVNPNTVLVDGPRDVLDSMITVNTDVV
                     SISGAEGDIATRVRIVGLPDGVSLLEPPSGMVDVVVQIRQRGVQQPLPGQPVSVVNLT
                     PGLVADVSPDTVILTVIGNEQELQNLTSSSLSIQVDARGLQPGTYNLRPT"
BASE COUNT          511 a          870 c          815 g          481 t
ORIGIN      
        1 gcagcgtggc ttcgtcgccg aggcgcgtgc cgaactcatc cagcgggccg acctcgagga
       61 acgggccggc aaggcgaagg aagcgattat cagcctccag cgggcggcgg atttgagctg
      121 gaacctcggt tttccccaag agacctttaa cctgtacgac cggatcctgg cgatcgaccc
      181 ggaagacgtt tccaaccgga gcgcgctggt gaacctctat ctgcaggttg ggcggctgag
      241 cgatgccgcc gagcaccaac gggccgtggt cgacctcgcg ctgaagaacg gtcgcaagca
      301 cgaagcggtg gccgcgctgc accaggtcat cggtctcaca cctgatgaca cgaccgctta
      361 ctaccagctg ggcgaagcgc tttcctccat gggcgagttc cagcaggccg agaaggtcta
      421 tcgccgcatc gtactgatga atcccgacga cgcggtggcc caggcgaagg caaccgcaat
      481 ggccgcgctc aaggagcagg cgcaaggtca ctaacgccca aaacacggcg tcccgaacaa
      541 cgtacgccgt gttaccatcg tttcatcgat tcgtccgccg gcgcatccac gcaatgtgat
      601 ctaaccaccg atgtcagaat tgacctggct actttcacga cttggagagc cgcggtcaat
      661 cgttgacatt gccgtcgtaa ccgggttgct tttctggctg ctctgggtcc tccagggtac
      721 ccgcgccacc caattgttgc ggggcgcggc gatcttcctg atcggggcta tcctgcttgc
      781 ccggacgctt gatcttcggg ccctcggctg gctcatcgac aacatgtggc cagcggtgat
      841 cgtcgccatc ccgatcgtct tccagcccga gcttcgacgc gcgctcgaac aactcggcca
      901 taccggcacg tggctgcgca cgtcgctccc ggccggttcc gacgtcacca cgatgtcgag
      961 cacgatcgac gaagtcgtcc gagccgcagc ccagctctca cgactccgat acggcgcgtt
     1021 gatggttctg gagcgcgaga ccggcctcca ggactacgcg gaccgcggcg ttgccatcga
     1081 tgcgctcgtc tcccggcagt tgctggtcac tatcttcttc ccgaactcgc cgctgcatga
     1141 tgccgctgtc ctgatgcgcg gtgatcggat cgtggcgggc agcgtggttc tccctctcag
     1201 cgagaacatc tcgccgatga gtaacctcgg cacgcggcat cgagccggta tcggcgtcac
     1261 cgaagattcc gacgccctgg cgatcatcgt ctcggaggag accggtcaga tctccgtcgc
     1321 acaccacggg cgcctcatcc gcaacctcga ccaggatcgc ctgcgccgcg tcctacgcac
     1381 tctgtcgcga gtcgagcgtg tcgaccggga cacccaggcg tccggtgtac gacgcgtcat
     1441 cgccgaacgc ctcgggcaga tcgccgagcg gcgcgggcgc caacggcccc ccgggacgca
     1501 tccgtccacg tcgaagatgt agccgtgcag caacccgacg agcgaaccag gaaatccttc
     1561 gcgactgagt ggggctcgcg cctgctcatt tcactgctcc tggcgatact gctctggggg
     1621 tgggtgacgg cgaccggtga tccggagacg acgcgacagt ttccgagcat tccgatctcg
     1681 gttgaaggac tcaaggaagg gttcgtcctg gtgggcgacg tcggatcggc cgaagtgcgc
     1741 gtgaccggac ccaggtctgc cgtcgaggac ctgctctcga cgcagattca ggcgtttctg
     1801 gatctcgatc agatatcaga gcctggcaca tacacagtgc ggattcaggc gccaaagccg
     1861 aacaacacct ggtccacggc gccgcgacca cggtcaatga atatcgtcgt cgagcgcgaa
     1921 acgacaaagg catttccggt tgatcccaag ccatcaggta gcctcggaac caaccagcaa
     1981 gttgaaacgg tcaatcccag cgcgtccgag gtcactgtca cggggccgaa ctcattggtc
     2041 gagcgagtca accaggtcgt gctgccgatt gacatcgcca accggacgac cgattttgca
     2101 agtgtcttca cgccggtccc gatcgacaag agcggtcagc ccattgctgg gctaacgctg
     2161 aacccatcga ccatatccgc cacggtcgag atcacgacgc ggggcaagcg cgttgccgtc
     2221 atcgcccaga tcgaaggcga gccgtccagc ggttttgagg tggtggaccg tttggtgaat
     2281 ccgaacaccg tgctcgtcga cgggccccgc gatgtccttg actcgatgat tacggtcaat
     2341 acagacgtcg tgagcatcag cggcgcggaa ggcgacattg cgacgcgtgt gcgcatagtc
     2401 ggacttcccg atggagtgtc attactcgag ccaccatccg gcatggtcga tgtcgtggtc
     2461 cagatccggc agcgaggcgt tcaacaaccg cttcccggcc agccggtatc cgtggtgaat
     2521 ctgacgccgg gcctcgtagc agacgtctcg ccggacacgg tcatcctgac ggttatcggc
     2581 aacgaacagg agctgcagaa cctgacatcg tccagcctgt cgatacaagt cgatgcgcgc
     2641 gggcttcagc cgggcaccta taacctgcgc ccaacgg
//
LOCUS       DASSGX010000208         2943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1019568, whole genome shotgun sequence.
ACCESSION   DASSGX010000208 DASSGX010000000
VERSION     DASSGX010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1019568"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..630)
                     /locus_tag="VFI12_03645"
     CDS             complement(55..630)
                     /locus_tag="VFI12_03645"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HET7055528.1"
                     /translation="MTMLNPTIVKAIKANGLLKEGHYAYRSGQHSAWLIDRDQLLTEP
                     EFASHLGYAIAKHFFTDRIETVATPSIWGAGIAQWVAYFLQPRARIVHATPIDGEPTV
                     APILEGLIRDKRILLCDNLIMSGQTMGKFVKTIDNLGGQIVGLATIWNSAALEIGGHP
                     VFGLLNSLYEPRSPDDCDLCARGVPLEHIPY"
     gene            complement(708..1541)
                     /locus_tag="VFI12_03650"
     CDS             complement(708..1541)
                     /locus_tag="VFI12_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET7055529.1"
                     /translation="MSSATAEAPGVAAQAMVRIAGVHKFYGDLHVLRGVDLEIVPGEV
                     VCVIGPSGSGKTTLLRCINFLETYQQGRIYVDGQLVGYREVNDRLKPAKERDIARIRA
                     ETAMVFQSFNLFPHMTALKNITFGPLKVRNEPKSEVERRGHELLDRVGLLDKADAYPA
                     QLSGGQQQRVAIARALAMNPKVMLFDEVTSALDPELVGEVLGVMKQLAEAHGVTMVVV
                     THEMAFARDVADRVVFMDQGVIVEQGPPDEVFSNPKSDRLRSFLARFSGSHGIPAAAT
                     A"
     gene            complement(1555..2232)
                     /locus_tag="VFI12_03655"
     CDS             complement(1555..2232)
                     /locus_tag="VFI12_03655"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET7055530.1"
                     /translation="MRVVIDNADLIWIGLKLTLELSVVIIVLSTLFGTLVGVGLVYGN
                     FVVRSILRLYVDVIRGIPLLVLIFLIFYGLPALQISVFGHQVNTNIGRFETATLAFSL
                     FAAAHVGEIVRGALAAVPAGQTDAAKAIGLTFWPRLRHILLPQSIPVILPPWTNTAAE
                     LVKGTSLVTLVSMSDLMFATRKIAERTGDVIPLYSAAAIIYFVICFTISRAGVWLSGR
                     YRYGVAR"
     gene            complement(2236..2904)
                     /locus_tag="VFI12_03660"
     CDS             complement(2236..2904)
                     /locus_tag="VFI12_03660"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET7055531.1"
                     /translation="MRVVIDNADLIWIGLKMAVIALAIGCVIGLVCAFIRASGNRWAS
                     IPVTIYVEIIRNIPLLLIVFLFYFGLPQAFPRGSTARDWTLRLLPDAERTFIVALAIY
                     AGAYLTEIFTAGILSVGGRYLDAGRSLGLTRLELARYVTAPIMLRAVLPSLSNTFISL
                     FKDTSIAVAIATPELTMAARKISTNYFRVIEAWLTAGALYLVTSYAIALSLRFLERRI
                     KWSV"
BASE COUNT          561 a          908 c          982 g          492 t
ORIGIN      
        1 gcctcggcga agcgcggcag gcgaacgagc ggcgatttcg ggcgggacat ggcgctagta
       61 cgggatgtgc tcgagcggga ctccccgggc gcataagtcg caatcatccg ggctgcgggg
      121 ttcgtacagg gagttgagca gcccgaagac cggatgcccg ccgatctcga gtgcggcgga
      181 gttccagatc gtggcgaggc cgacaatctg cccgccgagg ttgtcgatgg ttttgacgaa
      241 cttgcccatc gtctggccgc tcatgatcag gttgtcgcag agcaggatcc gcttgtcccg
      301 gatcagccct tcgaggatcg gcgcaacggt cggctcgccg tcgatcggcg tggcgtggac
      361 gatccgggcg cgcggctgga ggaagtaggc gacccattgc gcgatcccgg cgccccagat
      421 cgagggcgtc gcgacggtct cgatccggtc ggtgaagaag tgcttggcga tcgcgtagcc
      481 gaggtggctg gcgaactcgg gctcggtcag gagctggtcg cggtcgatca gccacgcgct
      541 gtgctgcccg gagcggtagg cgtaatgccc ctctttgagc aggccgttgg ccttgatcgc
      601 cttgacgatg gtcggattca gcatcgtcat tgcccgaaaa tccctcccgg tctcgcagtc
      661 agcctgccag cgatcatacc cgacaaggaa ttcccggcgc gaattgccta tgcggtcgcc
      721 gccgccggga tgccatgact gcccgagaag cgggcgagga aggatcgcag gcggtcgctc
      781 ttcgggttcg agaagacctc gtccggcggc ccctgttcga cgatcacgcc ctgatccatg
      841 aagacgacgc gatcggcgac gtcccgcgcg aacgccatct catgcgtgac gacgaccatc
      901 gtcacgccgt gggcctcggc gagctgcttc atgacgccga ggacctcgcc gacgagctca
      961 ggatcgagcg ccgaggtgac ttcgtcgaag agcatcactt tcgggttcat tgccagagcc
     1021 cgggcgatcg ccacccgctg ctgctgcccg ccggaaagct gggccggata ggcgtcggcc
     1081 ttgtccagca acccgacccg atcgagcaac tcgtgcccgc ggcgttcgac ctccgatttc
     1141 ggctcgtttc gcaccttgag cgggccgaag gtgatgttct tcagggcggt catgtgcggg
     1201 aagaggttga agctctgaaa gaccatcgcg gtctcggccc ggatgcgcgc gatatcccgc
     1261 tccttggccg gcttcaaccg gtcgttgact tcgcgatacc cgacgagctg cccgtcgacg
     1321 tagatgcggc cctgttgata ggtttccagg aagttgatgc agcgcagcag ggtcgtcttg
     1381 ccggagccgc tcggcccgat gacacagacg acttctccag gcacgatctc cagatcgacg
     1441 ccgcgcagca catgcaggtc gccgtagaac ttgtgcacgc cggcgatccg caccatcgcc
     1501 tgggccgcaa ctcccggcgc ttcggccgtt gctgagctca tggatatccc ccgattaccg
     1561 tgccacgccg tagcggtatc gcccgctgag ccagaccccg gcgcgcgaaa tcgtaaagca
     1621 gatcacgaag tagatgatcg cggccgcgct gtatagggga atcacatcac ctgttcgctc
     1681 ggcgatcttg cgcgtcgcga acatcaggtc gctcatgctg accagcgtga ccagcgacgt
     1741 acccttgacc agctcggccg ccgtattcgt ccagggtggc aggatcaccg gaatcgactg
     1801 gggcaggagg atgtggcgca accggggcca aaaggtcagc ccgatcgcct tggccgcatc
     1861 cgtctgcccg gccggaaccg ccgccaatgc cccgcgcacg atctcaccga cgtgcgccgc
     1921 cgcaaagagc gagaaggcca gcgtcgcggt ctcgaaccgg ccaatgttgg tgttgacctg
     1981 gtggccgaag accgagattt ggagcgcggg gaggccgtag aagatcagaa agatcaggac
     2041 gagcagcgga atgccgcgga tcacgtcgac atagaggcgc aagatgctcc gcaccacgaa
     2101 attgccatag acgagcccga cgccgaccag cgtgccgaac aacgtgctga gcacgatgat
     2161 cacgaccgac agctcgagcg tgagcttgag gccgatccag atcagatccg cgttgtcgat
     2221 gacgacccgc atgacctaaa cgctccattt gatccggcgc tcgaggaagc gcaggctcag
     2281 ggcgatggcg tacgacgtga ccaggtagag cgcgccggcg gtcaaccacg cttcgatcac
     2341 gcggaaatag ttggtgctga tcttgcgtgc cgccatggtc agctccgggg tggcgatggc
     2401 cacggcgatc gaggtgtcct tgaagagcga gatgaacgtg ttgctgaggc tcggcagcac
     2461 ggcgcgcagc atgatcggcg ccgtgacgta tcgggccaat tcgagccggg tcagccccag
     2521 cgaccggccg gcatcgaggt agcggccgcc gacggagagg atgccggcgg tgaagatctc
     2581 ggtcaggtaa gcgccggcgt agatcgcaag cgccacgatg aaggtgcgct cagcatccgg
     2641 caggagccgg agggtccaat cacgggctgt gcttccgcgc gggaaagcct ggggaaggcc
     2701 gaaatagaac aggaagacga tcaggagtag cgggatgttg cggatgattt cgacatagat
     2761 ggtgaccggg atgctcgccc atcggttgcc tgacgcccgg ataaaggcgc agaccagtcc
     2821 aatgacacag cctatggcaa gcgcaatgac cgccatcttg aggccgatcc agatcagatc
     2881 cgcgttgtcg atgacgaccc gcatgaccta aacgctccat ttgatccggc gctcgaggaa
     2941 gcg
//
LOCUS       DASSGX010000209         2476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1456059, whole genome shotgun sequence.
ACCESSION   DASSGX010000209 DASSGX010000000
VERSION     DASSGX010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1456059"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="VFI12_03665"
     CDS             <1..748
                     /locus_tag="VFI12_03665"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET7055532.1"
                     /translation="LILSYHVIPDRFKMAYVGITALGVIPTFSRSAMIGWMLVVGYFF
                     IRAGFRAQIRRLAILAVVCVGVHFSPIWGDIQHSLQQRGTLNLNIVERIQFFTGGGRG
                     DDSSDERKAVATRAWELFGERPLDGWGTGASRRIEGFDVGTHNIYLAMLVDHGITGIF
                     VIPFLLLSALWGLNRKTIDIAAPWLIFMLLWGMFSHNVLEERYILLAVALTASMIASN
                     RTVAAESAEPAESSLPPFPATPRAGAVAVP"
     gene            745..1893
                     /locus_tag="VFI12_03670"
     CDS             745..1893
                     /locus_tag="VFI12_03670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7055533.1"
                     /translation="MKPVVAHVITGLKTGGAEMMLARLLERHEQSRFRPVVYCLTDNG
                     PVADRIRSLGVPVHALGMRRGIPDPFTVLRLARALRRERAVAVQTWLYHADLIGGLAG
                     RLAGIPVAWGVHNSRLRGNAAGTGLRAVIWLCARISRWAPTCIVCVAESSRTAHAEIG
                     YPVRKMHVIPNGFDLAQFRPNPEARQRTRVELQIGPKDPVVMIAGRFVPLKNHRLFVE
                     AAGIVADRVPSAHFVMAGDGLDEANAELTGWIAGTGHHSRFRLLGRRSDMADLLAATD
                     VVALTSNTEAFPLVIGEAMATGVPCVSTDVGDARSLISDTGRVVPMGDTLGFATGLLE
                     LIELPEDRRRALGCAARIRMEAEFDLGRIAARYDALWQSLAAGERPCG"
     gene            1884..>2476
                     /locus_tag="VFI12_03675"
     CDS             1884..>2476
                     /locus_tag="VFI12_03675"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7055534.1"
                     /translation="MRLMYVITRGDTIGGAQMMVRDLAIGTAERGHEVVVVTGIGGPL
                     TEQLAARGIETRICEGMLREIDPRQDIRAVRAMTALIHELHPDLVTTHSSKAGVVGRI
                     AARRAGVPAMFTAHGFAFTGGVSQPKRTIYRLIERGMERFAARIVCVSDYDRNLAITA
                     GMSPDRLVTIHNALPDIDPSLRAHPDQGSPVRIVKVAR"
BASE COUNT          376 a          858 c          806 g          436 t
ORIGIN      
        1 gctgatcctc agctatcacg tcattcccga ccggttcaaa atggcctacg tcggcatcac
       61 cgccctcggc gtgattccga ccttctcccg ctcggcgatg atcggctgga tgctggtcgt
      121 cggctacttc ttcatccggg ccggtttccg cgcccagatt cgccgcctgg ccatcctggc
      181 cgtcgtctgc gtgggcgtcc acttttcgcc gatctggggc gatattcagc actcgctcca
      241 gcagcggggc acgctcaacc tcaacatcgt cgagcggatc cagttcttca cgggcggcgg
      301 gcggggtgac gattcgtccg acgagcgcaa ggcggtcgcg acacgggcgt gggaactctt
      361 cggcgagcgc ccactcgatg gctggggcac cggggccagc cgccggatcg agggattcga
      421 cgtcggcacc cacaacatct acctcgcgat gctcgtcgac cacggcatca ccgggatctt
      481 cgtcattccg ttcctgttgc tgagcgcgct ttggggcctg aaccgcaaga cgatcgatat
      541 cgccgcgccg tggctcatct tcatgttgct gtggggcatg ttcagccaca atgtgctcga
      601 ggagcgctac atcctgctcg ccgtcgcact gacggcatcg atgatcgcat cgaaccggac
      661 ggtcgcagct gaatcggccg aaccggccga atcgtccctg ccaccgttcc cggccacccc
      721 ccgggcaggc gccgtggccg tgccatgaag ccggtcgttg cccacgtcat caccggcctg
      781 aagaccggcg gcgccgagat gatgctcgcc cggctgctgg agcggcacga gcagtcccgc
      841 ttccggccgg tcgtctactg cctgaccgac aacgggcccg tggccgaccg catccggtcg
      901 ctcggcgtgc cggtccacgc gctcggcatg cgaagaggaa tccccgaccc gttcacggtt
      961 ctgcgactcg cccgggcgct caggcgggag cgagccgtgg ccgtgcaaac ctggctctac
     1021 cacgcggatc tgatcggcgg gttggctggc cgactcgccg ggatccctgt cgcctggggc
     1081 gtccataaca gccgattgcg cgggaatgcc gccggtaccg ggctgcgcgc ggtgatctgg
     1141 ctctgcgcgc ggatatcgcg ctgggctccc acctgcatcg tctgcgtggc cgagtcgtcc
     1201 cgcacggccc acgcggagat cggttatccg gttcgaaaga tgcacgtcat cccgaacgga
     1261 ttcgaccttg cgcagttccg gcccaacccg gaggcgcggc agcggactcg ggttgagttg
     1321 caaattggcc cgaaggaccc ggtcgtcatg attgccggcc gcttcgtgcc gctcaagaat
     1381 caccggctct tcgtcgaggc cgccgggatc gtcgcggatc gcgtcccgag cgcgcatttc
     1441 gtgatggccg gcgatggtct ggacgaggca aatgccgaac tgaccggctg gatcgccggc
     1501 actggtcacc actcgcgatt tcggctcctt ggtcgccggt ccgacatggc tgacctgctc
     1561 gccgccaccg atgtcgtcgc cctgacctcg aacaccgaag catttccgct ggtcatcggc
     1621 gaggccatgg cgaccggcgt cccctgcgtc agcaccgacg tcggcgatgc ccgctcattg
     1681 atctcggaca ccggccgtgt cgtccccatg ggcgatacgc tcgggtttgc cacgggtctg
     1741 ctggaactca tcgagctgcc ggaggaccgc cggcgagcgc tcggatgcgc ggcccggata
     1801 cggatggagg ccgagttcga tctcggcagg attgccgccc ggtacgatgc gctctggcaa
     1861 tcgcttgcag cgggagagcg gccatgcggt tgatgtacgt catcacccgg ggcgacacga
     1921 tcggtggggc acagatgatg gtccgtgacc tcgctatcgg gaccgccgag cgcggtcacg
     1981 aagtggtcgt cgtcaccggc atcggcggtc cgctgacgga gcaactggcc gcgcgcggga
     2041 tcgagacccg gatctgtgag gggatgctcc gcgagatcga cccgcggcag gacatccgcg
     2101 ccgtccgggc aatgaccgcg ttgatccacg aactacatcc tgatctcgtc acgacccact
     2161 cgagcaaggc cggcgtggtc ggccggatcg cggcacgccg ggccggcgtc ccggcgatgt
     2221 tcacggccca cggcttcgca ttcaccggcg gtgtctcgca gccgaagcgg acgatctacc
     2281 gactgatcga gcgcggcatg gagcggttcg ctgctcgcat cgtctgcgtc tccgactacg
     2341 accggaacct ggcgatcacg gccggcatgt cacccgatcg gctcgtcacg atccacaacg
     2401 ccctgcccga catcgatccg tcactcaggg cgcatcccga tcagggctcg ccagtccgga
     2461 tcgtcaaggt cgcccg
//
LOCUS       DASSGX010000210         3325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_474984, whole genome shotgun sequence.
ACCESSION   DASSGX010000210 DASSGX010000000
VERSION     DASSGX010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474984"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            70..1074
                     /locus_tag="VFI12_03680"
     CDS             70..1074
                     /locus_tag="VFI12_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET7055535.1"
                     /translation="MASIDRVALGQTGLVVPPIAIGCAELGDMPEAFAYAVSEEDALA
                     TVRAALDSPLNYIDTAAWYGNGESERRIGLVLRERGGLPEGAILETKAGRDPNGDDYR
                     PATVKARFERSLELLGVDKVHICFIHDAEWTTFDAVMAPGGVMEILQDYKRQGVIGAL
                     GIASGPVELAMQYIQTGMLDILLTHNRYTLLNRAADAVQKEAKRRGMGVVNAAPYGSG
                     MLAKGPDAYARYAYQDAPVEMIETTRNIEAICAKHGVPLAAAALQFSMLDPNVDVTIV
                     GMSRAERVQQTIDLATIEIPQALWDEIRDLPTYDDDPELNRWKASATQPVFVASMGDS
                     "
     gene            1101..2303
                     /locus_tag="VFI12_03685"
     CDS             1101..2303
                     /locus_tag="VFI12_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HET7055536.1"
                     /translation="MAEFDTLIAGGHVIDPAQNRSGLADVAIKDGTIATVEPGIDRAR
                     AANVIDAAGQYVTPGLIDLHTHVYWGVTYWGIEPDPVAARSGVTTWLDVGSAGGYTFP
                     GFRRWISEPAKSRVFALLNMSSIGLVAPTWELSNIDYCDIDLGQMIVERNRDIILGIK
                     ARIDASTTRGTGIEPLKRARELADKVELPLMVHIGGAPPTLAEIVEYLRPGDILTHCF
                     TGQPNKIVDDAGAVIPSIKDLHDRGLVLDIGHGAGSFSFDTAEAMIGQGILPDVISTD
                     IHQLAIQGPCFDMPVTLSKFLNLGMTIEQVIACATINAAKAIRLPELGTLCAGAPADV
                     ATFRLDQGEFTFQDVHMNQRSGDKLLVNTMTMVDGDILPVIEDKPLQPWAKLPAHQAG
                     KVIPVRQV"
     gene            complement(2767..>3325)
                     /locus_tag="VFI12_03690"
     CDS             complement(2767..>3325)
                     /locus_tag="VFI12_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HET7055537.1"
                     /translation="ALQRRTKKTAIYVTHDQSEAFAISDRIFVMNQGRIEQSGTQLDI
                     YLRPASEFVAHFVGDNNTLNGTVLAIDGASGETRTATVKTANATLTAFAPPDIRVGDE
                     VLALARPENIEILTNGAEPGGADILTGTVDQIVFEGPTVRLTVDVDGTPIKVTSSGLE
                     RLSLLNTESMRVRLKLQDVTLVKPE"
BASE COUNT          579 a         1068 c         1110 g          568 t
ORIGIN      
        1 gtcagcgaat gacgtcggcc tatgcgatca tgcccacatg tacccgccca tcccacacag
       61 gagagaatca tggcctcgat cgaccgggta gcgctcggcc agaccggcct tgtcgtgccg
      121 cccattgcca tcggctgcgc ggagctcggg gacatgcccg aggcctttgc ctacgccgtc
      181 tcggaagagg acgccctggc cacggtccgc gcggcgctcg acagcccgct caactacatc
      241 gacaccgccg cctggtacgg caacggcgag agcgagcgcc ggatcggcct tgtcctgcgg
      301 gagcggggcg gcctgccgga gggtgcgatc ctcgagacca aggccggccg ggatccgaac
      361 ggggacgatt accggccggc aacagtcaag gcccgcttcg agcggagcct cgagctgctc
      421 ggcgtcgata aggtccacat ctgtttcatc cacgacgccg agtggaccac cttcgatgcc
      481 gtcatggcgc cgggcggcgt gatggagatt ctgcaggact acaaacgaca gggcgtgatc
      541 ggcgcgctcg ggatcgcgtc cggcccggtc gaacttgcga tgcagtacat ccagaccggc
      601 atgctcgata tcctcctgac ccacaaccgg tacacactcc tcaaccgcgc ggcggatgcg
      661 gtccagaagg aggccaagcg gcgggggatg ggcgttgtca atgccgcgcc gtacggctcc
      721 ggcatgctgg ccaaggggcc ggacgcctac gcacggtacg cgtaccagga cgcaccggtg
      781 gagatgatcg agacgacccg gaacatcgag gcgatctgcg cgaagcacgg cgtcccgctg
      841 gcggcggccg cgctccagtt ctcgatgctt gacccaaacg tggacgtgac catcgtcggc
      901 atgagcaggg cggagcgcgt tcagcagacg atcgatctcg cgacgatcga gattccacag
      961 gcgctttggg acgagatcag ggatttgccg acgtacgatg acgatccgga gttgaaccgc
     1021 tggaaggcat ccgcaacgca accagtgttc gtggcatcga tgggtgattc gtaagtctaa
     1081 gttggacagg agagcttccg ttggctgaat tcgatacgct gatcgccggt ggtcatgtca
     1141 ttgatcctgc ccagaaccgg tccgggctgg cggacgtcgc gatcaaggac ggcacgatcg
     1201 cgaccgtcga gccgggtatc gaccgggcgc gggccgcgaa cgtgatcgat gcggccgggc
     1261 agtacgtgac gccgggcctg atcgacctgc acacgcacgt ctactggggc gtgacctact
     1321 ggggcatcga gcccgatccg gtcgcggccc gcagcggcgt cacgacgtgg ctggatgtcg
     1381 gtagcgccgg cggctacacc tttccgggct tccgccggtg gatatcggag ccggcgaaat
     1441 cacgcgtctt tgcgctgctc aacatgtcct cgatcggcct ggtcgcgccg acgtgggagc
     1501 tctcaaatat cgactattgc gacattgacc ttggtcagat gatcgtcgag cggaaccgcg
     1561 acatcattct cggcatcaag gcccggatcg atgcaagcac aacgcgcggc accgggatcg
     1621 agccgctcaa gcgcgcgcgc gagctggccg acaaggtcga gctgccgctg atggtccaca
     1681 tcggcggggc cccgccgacg ctggccgaga tcgtcgagta cctgcggccg ggcgatatcc
     1741 tgacccactg cttcaccggc cagccgaaca agatcgtcga tgatgcgggg gcggttatcc
     1801 cctcgatcaa ggacctgcac gatcgcggtc tggtgctcga catcggccac ggcgccggct
     1861 cgttcagctt cgacacggcc gaggcgatga tcgggcaggg gattctgccg gacgtgatca
     1921 gcaccgacat ccatcaattg gcaatccagg ggccgtgctt cgacatgccg gtcacgctct
     1981 ccaagttcct gaacctcggg atgacgatcg agcaagtcat cgcctgcgcg acgatcaatg
     2041 cggccaaggc gatccggctg ccggagctcg gcacgctctg tgccggcgcg ccggcggacg
     2101 tggcgacatt ccggctggat cagggcgagt tcacattcca ggacgtccac atgaaccagc
     2161 gcagcggcga caagctcctg gtcaacacca tgaccatggt tgacggcgac atcctgccgg
     2221 tcattgagga caagccgctc caaccctggg ccaagctccc ggcgcaccag gcaggcaagg
     2281 tgattccggt gcggcaagtc tgacgaggcg agaagtcgag gagtcgcact gtaggggcag
     2341 atggcatttg ccctccgttc ctcaacggcc aggtcacatg cggacgtgcg tgcccgatat
     2401 cgcccgggcg taaacacccg ggctgacctc tgaacccggc tgaagccggc tggatggggc
     2461 acgctcctga accattcgcc tcgaacgtag cgcggtgcgt gagccccgcg accgggcccc
     2521 gtcccgatcc ggtcgttccc gggaaccaac ctccgtggcc gcatcgccgc catccaccct
     2581 atccggggga atcaccggcg cggggcttcc gcaccgcgct acgtcgtggt tcgggtacat
     2641 gcgaacgggc cggttggaga gggcgggcag atgccatctg cccctacaat tccgcgcctt
     2701 cgcgcgttcg cgcgttcgcg cgttcgcgcc ttcgcgcctt cgcgcctctt tcggctgctc
     2761 gcctttctac tcaggcttga ccaatgtcac gtcctgcagc ttgaggcgga cgcgcatgga
     2821 ttccgtgttg agcagggaga ggcgttcgag gccgcttgag gtgaccttga tcggggtgcc
     2881 gtcaacgtcg acggtgaggc ggaccgtcgg accctcgaag acgatctgat cgacggtgcc
     2941 ggtgagaatg tcagcgccac ccggctcggc gccgttcgtc aggatctcga tgttctccgg
     3001 ccgggcaagc gccaggacct catcgccaac ccggatatcc ggcggagcga aggcggtcag
     3061 cgtggcgttc gccgtcttga cggtggccgt gcgggtctcg ccgctggccc cgtcgatcgc
     3121 gaggacagtg ccgttcaacg tgttgttgtc cccgacgaag tgggccacga attcgctggc
     3181 cgggcgcagg tagatatcga gctgcgtgcc ggattgctcg atccggccct ggttcatcac
     3241 gaagatgcga tcggagatcg cgaaggcttc ggactggtcg tgcgtgacgt agatcgcggt
     3301 ttttttggtt cgccgttgca gggcg
//
LOCUS       DASSGX010000211         2894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1238146, whole genome shotgun sequence.
ACCESSION   DASSGX010000211 DASSGX010000000
VERSION     DASSGX010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1238146"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VFI12_03695"
     CDS             complement(<1..219)
                     /locus_tag="VFI12_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055538.1"
                     /translation="MRSISTLANSIERSGIRDLMDAAREMGGEIVHLEVGEPSFTTPA
                     HIIEQAFEATSAGATRYTQSGGVLPLRQA"
     gene            complement(300..1280)
                     /locus_tag="VFI12_03700"
     CDS             complement(300..1280)
                     /locus_tag="VFI12_03700"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HET7055539.1"
                     /translation="MSDRGIEVENAWSAVPKAALATIALCVLAVLVWQGITADGAPDP
                     MTPGLHHRAVVIDAGVLVFREGLEAILVLAAITSSFVGARARFRAPIGLGAGAAGLAS
                     IATWFIVIGIIGAINAPALHVQAATGLLAVLVLLVIMNWFFHRIYWTGWIAHHNRRRR
                     DIMAVGGSSVTLGLILLGFTAVYREGFEIVLFLQTLRLQAGSGTVLEGVAIGLALTAA
                     VGVLTFMANRKLPYKRMLVLTGIMLGFVLVVMVGESVQEMQLAGWVPTTEVNLPIPDW
                     MGVWFATFPTVETLLGQLLAAVLVIGSYFVARETRLVRAPGSPSGAGRAD"
     gene            complement(1480..2655)
                     /locus_tag="VFI12_03705"
     CDS             complement(1480..2655)
                     /locus_tag="VFI12_03705"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7055540.1"
                     /translation="MNHSYSFEAMVTHGDDGIEETLDVAPPIHQTSTFVAGTAEEFAR
                     QATDPRPDAYYTRAGNPTHRRVEAILAKLEGAEAGLATASGMSAITCSILAHVAAGDH
                     VVAQKNHYMAASKFATDILPRFGVQSSLVDQTDPEAFAGAIRPETKVIFVETPSNPTG
                     QITDLRAIADLAQPRGIVTICDNTFASPINQQPISHGIDVVVHSATKYLGGHHDLIAG
                     AVLGSRARVEQIWRFTNMFGPTLGPIDAWLLLRGLRTLPLRVRHHNEAGCAVARHLEQ
                     HPKIEAVHYPGLPSHPDHSVASRQMSGYGATFSFQVRGGYQDTQRFMSNLEMVKQAVS
                     LGGFETLAVHAAAMWGGSLSDEQVREAGVEPNMVRISIGLETAEDVITDLDHALEVV"
BASE COUNT          512 a          892 c          979 g          511 t
ORIGIN      
        1 cgcctggcgg agggggagga cgccaccgct ctgggtgtac cgggtcgcgc cggcggaggt
       61 cgcctcgaac gcctgctcga tgatgtgggc cggggtcgta aagctgggct ccccgacctc
      121 gaggtggacg atctcgccac ccatctcgcg cgcggcatcc atcagatcgc gaatgccgga
      181 ccgctcgatc gagttcgcca gcgtagaaat agaacgcacg tgtgcctccc gggatgatgc
      241 tcagttgccg gggggattgt aggggagaca gcggcttcgg tggccggcgc gcggcggagc
      301 tagtccgcgc gacccgctcc actcggcgat cctggcgcgc gaacgagccg ggtctcccgc
      361 gcaacgaaat acgacccgat gacgagcact gcggccagca gttgcccgag caacgtttcc
      421 acggttggga acgtcgcgaa ccagacgccc atccagtccg ggatgggcag gttgacttcg
      481 gttgtgggta cccagccggc aagctgcatc tcttgcacgc tctcgccaac catgacgacg
      541 aggacgaagc cgagcatgat cccggtgagg acgagcatcc gcttgtacgg tagcttccgg
      601 ttcgccatga acgtgagcac gcccacagct gcagtcaggg ccagaccgat cgcgacccct
      661 tcgagcacgg ttccggagcc cgcctgcaag cggagcgttt gcaggaagag gacgatttcg
      721 aaaccctcgc ggtagacggc ggtgaagccg agcaggatca ggccgagggt cacgctcgat
      781 cctccgacgg ccataatgtc ccggcggcgc cggttgtgat gcgcgatcca gccggtccag
      841 taaatccggt ggaagaacca gttcatgatc accagcagca ccaggaccgc caacaacccg
      901 gtggccgcct ggacgtggag ggcaggggcg ttgatcgcgc cgatgatccc gatcacgatg
      961 aaccaggttg cgattgacgc aaggccggcc gctcccgctc cgagaccaat cggcgcccgg
     1021 aagcgggcgc gagcgcccac gaagctcgag gtgatcgcgg ccaggacgag aattgcctcg
     1081 agaccttccc ggaagacaag cacaccggca tcgatgacga cggcgcggtg gtggaggccg
     1141 ggcgtcatcg gatcgggcgc gccatcggcg gtaatgcctt gccagacgag caccgccagc
     1201 acgcagagcg cgatcgtggc cagtgcggcc tttggcacgg cgctccaggc gttctccact
     1261 tcgattccgc gatcgctcac ctggtattcc tcctggaacg atatctgact aattcagtcg
     1321 gatattacgc gccacggttc gcatgatcaa gtggcggccc atttcgcggc gtcactgatg
     1381 ccggttccgg gcatggcgat ggaccaggcc cgggcgtatt tccgggatgt tgtgctctac
     1441 agagggaact gcccgcttat caaggcggac agcgaatcgt cagactactt cgagggcgtg
     1501 gtcgaggtcg gtgatcacgt cctcggccgt ttccaggccg atcgagatgc ggaccatgtt
     1561 gggttcgaca ccggcctcac ggacctgctc gtcactcagc gatccgcccc acatcgcggc
     1621 ggcgtggacg gccagcgtct cgaatccgcc cagcgagacc gcctgcttga ccatctccag
     1681 gttggacatg aaccgctggg tgtcctggta gccgccacga acctggaagc tgaaggtcgc
     1741 gccgtagccg gacatctgcc ggctcgcgac cgagtgatcc gggtggcttg gcaggccggg
     1801 gtaatggacc gcctcgattt ttggatgctg ctcgaggtgc cgggccaccg cgcagcccgc
     1861 ctcgttgtgg tgccggaccc gcaggggcag cgtccgcaac ccgcgcaaga gcagccaggc
     1921 atcgatcggc ccgagcgtcg gcccgaacat gttggtgaat ctccagatct gctcgacccg
     1981 cgcccgcgag ccgagcaccg cgccggcgat caggtcgtgg tggccgccaa ggtatttggt
     2041 cgccgaatgg acgacgacat cgatcccgtg ggaaatcggc tgctggttga tcggcgaggc
     2101 gaaggtgttg tcgcagatcg tgacgattcc gcgcggctgt gccagatcag caattgcgcg
     2161 caggtcggtg atctggccgg tcggattcga cggcgtttcg acgaagatca ctttcgtctc
     2221 cggccggatc gcccctgcga aggcctccgg atcggtctga tcgacgaggg aggactgcac
     2281 gccgaagcgc ggcagaatgt ccgtggcgaa cttcgaagcc gccatgtaat ggttcttctg
     2341 ggcgaccacg tgatcaccgg ccgcgacgtg ggcgaggatc gagcaggtga tcgcgctcat
     2401 cccggatgcc gtggcgagtc cggcttccgc gccttccagc ttggcgagga tcgcctcgac
     2461 gcggcggtgg gtcgggttgc cggcgcgggt gtagtaggcg tcgggtcggg gatcggttgc
     2521 ctgccgcgca aactcctcgg cggttccggc gacgaaggtc gacgtctgat gaatcggcgg
     2581 cgcgacgtcg agggtctcct cgatgccgtc gtcaccatgc gtgaccattg cttcgaacga
     2641 atacgagtgg ttcacgattc ccgctttctg aatgagccgc gtacgcgttg acgcgcatgc
     2701 agtctaccgt tgattctcgg gtaatgatgc cgtacattcg ttctaatcgg atggcacgga
     2761 ggagcgaatc atgcagtttg gatacacgat cgtctatgtt ccggatgtcg aggcggcggt
     2821 ctcgttctat gagcgggcct tcgggttcac gaagcgattc aacgccgacg attgggatta
     2881 tgcggagctc gaaa
//
LOCUS       DASSGX010000212         4561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_258707, whole genome shotgun sequence.
ACCESSION   DASSGX010000212 DASSGX010000000
VERSION     DASSGX010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4561
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258707"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1785)
                     /locus_tag="VFI12_03710"
     CDS             complement(<1..1785)
                     /locus_tag="VFI12_03710"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7055541.1"
                     /translation="MEAEPAWQPETGTFGDLLRQLRIASALSQEALAERAGISTNAVS
                     HLERGVATRPHLETVRLLADALDLDRAGRAALASAARPPVTSPEAADALIADMTATGP
                     SLPIPPTSLIGREQDIARLAALLDRDDVRLVTLTGPGGVGKTRLALAEATARRTDFAN
                     GVWFIDLIPVADAGMVLRAIARALGLRDEGRAPIQERLQTFLRTRQVLLVLDNLEHVI
                     AAGPAIAELLTGADGLRILATSRVRLHVSGEHEVVIAPLPAPPETTGMTAATALDSPA
                     VRLFADRAAAAGAGFALSDASAPAVSEICARLDGLPLAIELAAARMKLFSPQGLLEGL
                     DERFALLRDGARDSAARHHSLWDAIAWSYDLLTSEEQLVFRRLAVFTGGMTLAAACEV
                     AELDATETVQVVSALVDKSLLQPSVGPGGDSRFVMLETIREFALRQLRDAGKEQAVRE
                     RHAAYFASFAERAAPNVCGPDEVLWLDRCEAELGNFRAALAWSTGESGDPVYAMRIGS
                     ALWWLWQTRIGMAEGKSWLERAADRTIGHDTGARAMALATAGALAAFQADYAAAERNA
                     LAAIDLTTGVDNSFVSAWPPFILGLTWLF"
     gene            2029..2619
                     /locus_tag="VFI12_03715"
     CDS             2029..2619
                     /locus_tag="VFI12_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055542.1"
                     /translation="MHTSVFARFAAVLTVMGLLLAMASPVSAGNPEKVAFHEEFDENV
                     CGIDVHTVVDGWTIFQIQDYVIQADDPSTQDDFWIGVTVNHSDVTWTNSAGVTIALAH
                     RENVKDIDLVDNGDGTWTYSYAVVGMPYRLRSGNQVVLMEAGRITFVAVIFLGDLSTQ
                     ADDEITSFAITSISGPHPLSESDFAIFCDTFTKFMG"
     gene            2683..3273
                     /locus_tag="VFI12_03720"
     CDS             2683..3273
                     /locus_tag="VFI12_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055543.1"
                     /translation="MRRTLFSRLAVILTILGLLATMVSPVSAGNPEKIIIHQEFDEEL
                     CGIPVHTTVDGFSMLHIQDYVIQADDPAVQDDFWIGVIQNHFNVTYTSGTGATMFQME
                     RNTTQEDSLVDNGDGTWTYTYSVNGQPLRLKGGNGSVVADVGQISVSIVLYFGDLSTQ
                     ADNSFVSEEITKVAGPHPIAESDFALFCEVVTDILG"
     gene            3440..4369
                     /locus_tag="VFI12_03725"
     CDS             3440..4369
                     /locus_tag="VFI12_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055544.1"
                     /translation="MWAHDATPAASTQPLIVYETIDSGGIISIWTMNSDGTEPVKHLG
                     SQTLSGKHPVWSPDGSSIIFINNVDGSIWTMNADGSDQTELLACADLCDEMDYPSWSP
                     DGSKVVFLAYSDESAGPPARSSLMTLDLKSGDLTTLADATLPRILDTPQWSPDGQTIV
                     LAMNELDDEFNQTGAALATMPASGGELTWLTEFSLYAYYPTWNAVTNQIVFGQEPVLW
                     SGASDADTQPLNLWVINPDGAGLEAITDLAEGEALVQSAWTPDGTRIIATLMDRNGPR
                     LVWVDPASGEITKMPTTAGNLYPRLQPVGKSPD"
BASE COUNT          862 a         1512 c         1410 g          777 t
ORIGIN      
        1 aaaaagccag gtcaagccga ggatgaatgg cggccaggca gacacaaagc tgttatccac
       61 gccggtggtg aggtcgatcg cggcgagggc gttacgttcg gcagccgcgt aatccgcctg
      121 gaatgcggcg agggcccccg cggtcgccaa cgccatggcc cgcgcgcccg tgtcatggcc
      181 aatggtgcgg tcggccgctc gctccagcca ggatttcccc tcggccatgc cgattcgggt
      241 ttgccacagc caccacaggg ctgacccgat gcgcatcgca tagaccggat cgcccgactc
      301 gcccgtgctc catgcgagcg ccgcccggaa attgccaagc tcggcctcgc agcggtccag
      361 ccacaacacc tcatcgggac cgcacacatt cggcgccgct cgctcggcaa aactcgcgaa
      421 gtaggcggcg tgccgctccc ggaccgcctg ctccttgccc gcgtcccgca attggcgcag
      481 cgcaaactcg cggatcgtct ccagcatgac gaagcgggaa tcgccgcccg gtccaacgct
      541 gggctggagc aggctcttgt cgaccagggc ggagacgacc tgaaccgttt cagtggcatc
      601 aagctccgcc acctcgcagg ccgcggccag ggtcatgccg ccggtgaaca cggccagccg
      661 gcgaaacacg agctgctcct cgctcgtcag caagtcgtag ctccaggcga tcgcatccca
      721 gagcgagtga tgccgggcgg cgctatcccg cgcgccatca cgcagcaggg cgaaccgctc
      781 gtccagcccc tcgagcagcc cctgcggcga aaacagcttc atccgcgccg cggccagctc
      841 gatggccagc ggcagcccat cgaggcgcgc gcagatctcg ctgactgccg gggccgacgc
      901 gtcggagagg gcgaagccgg cgccggccgc ggccgcgcga tccgcaaaga ggcgcaccgc
      961 cggcgagtcc aacgccgtcg ccgcagtcat gcccgtcgtt tccggaggcg ccgggagcgg
     1021 cgcaatgacg acctcgtgtt ctcccgagac atgcaagcga acccggctgg tggcgaggat
     1081 gcggaggccg tccgcgcccg tcagcagctc ggcgattgcc ggtccggcgg cgatcacgtg
     1141 ctcgaggttg tcgaggacga gcagcacctg gcgcgtgcgc agaaaggtct ggagccgctc
     1201 ctggatcggg gcgcggccct catcgcgcag gccgagggcg cgggcgatcg ctcgcagcac
     1261 catgccggca tccgcgacgg ggatcaggtc gatgaaccag acgccgttgg cgaagtccgt
     1321 tcggcgcgcc gtggcctcgg ccagggccag acgggtcttg ccgacgccgc cgggaccggt
     1381 cagggtgaca aggcgaacgt catcccggtc gagcagggcg gccagccggg caatgtcctg
     1441 ctcccgcccg atcagcgagg tggggggaat cggcaggctc gggccggtcg cggtcatatc
     1501 ggcgatcagg gcatcggcgg cctcgggact tgtgaccggg ggccgggcgg cagatgccag
     1561 cgccgctcgc ccggcccgat cgaggtcgag ggcatccgcg agcaggcgca cggtttcgag
     1621 gtgcggccgc gtcgcgacgc cgcgctcgag atggctgacg gcgttggtgc tgatccccgc
     1681 ccgctcggcc agggcctcct gcgagagggc cgacgcaatc cgaagctggc gcaagagatc
     1741 gccgaacgtc ccggtttcgg gttgccaagc cggctctgcc tccatggcta cagcccctgc
     1801 ctagtgtcat tcggtccgga tttcccgata gtacggcgct atcccgccaa acacaatact
     1861 tgtcaaaaga aagtatgtgg gcaggctgtg gccaacgtgt gggtatcccg cgtggttgcc
     1921 gccgcactcc ctccgcaaag tgaaggcaca tcgcacatgt agggccccat caggacgtcg
     1981 gcgccctgag tctgcgctcg gccgcttgcc actgaaagga acaccccgat gcacacgtct
     2041 gtgtttgctc gattcgccgc tgtgcttacc gtcatgggtc tgctgctcgc catggccagc
     2101 ccagtaagcg ctggaaatcc ggagaaggtc gccttccatg aggagtttga tgagaacgtc
     2161 tgcggcatag atgtccacac cgtagtcgac ggctggacga tttttcagat ccaggactac
     2221 gtgattcagg ctgatgatcc gagcacacag gacgacttct ggatcggggt gaccgtgaat
     2281 cactccgacg tcacctggac taactccgct ggagtgacaa tcgccctggc tcatcgtgag
     2341 aacgtcaagg atatcgacct cgtcgacaac ggagacggca cctggacata ttcctacgcg
     2401 gtggtcggca tgccataccg gctacgctcc ggcaaccagg tcgtgcttat ggaggccggc
     2461 aggatcacct ttgtggccgt catctttctg ggcgatctct ctacccaggc ggacgacgag
     2521 atcaccagct tcgctatcac cagcatcagc ggtccacacc cactgtcgga aagcgatttt
     2581 gcgatcttct gcgacacgtt caccaagttc atgggctaag tcctgtcgac accgggcggg
     2641 ggatgcccgc ccggttgccc ccattcaaga aaggaacgcg tcatgcgcag gactctgttc
     2701 agccgtctgg ctgtaatact gaccatcctc ggattactgg cgaccatggt cagcccggtg
     2761 agcgccggaa atccggagaa gatcatcatc caccaggaat tcgatgagga gctgtgcggc
     2821 atcccggttc acaccaccgt cgatggcttc tccatgctcc atattcagga ctacgtcatc
     2881 caggcggacg atcccgcggt ccaggacgac ttctggatcg gcgtcatcca gaatcacttc
     2941 aatgtcacat acacaagcgg aaccggcgcg accatgttcc agatggaacg caacacgacc
     3001 caggaggatt cgctcgtcga caacggcgac ggaacctgga cctacaccta cagcgtcaac
     3061 gggcaaccgc tgcggctgaa aggtgggaac ggatcggtcg tggccgacgt tggccagata
     3121 tcggtctcaa tcgtcctcta ctttggtgat ctctcgacgc aggctgacaa ctccttcgtc
     3181 agcgaggaga tcaccaaggt cgccgggccg cacccaatcg ccgagagcga cttcgccctc
     3241 ttctgcgagg tcgtcaccga catcctgggc tgagcaatcg ccgacgaatg caactgagcg
     3301 ccgggcttat gcccggcgct acggatcgga ggtttccatg ggtgttcgtc cacgctccac
     3361 gtctcacgca tcacgacttc ccctgcattg gatcgcgatg atgatcatcg ccgttctttc
     3421 gttaggtatg ttcggctcag tgtgggcgca cgacgcaaca ccggccgctt caacgcaacc
     3481 gctcatcgtc tacgagacaa tcgactcggg cggcatcatt tcgatctgga ccatgaacag
     3541 cgacggaacc gaacccgtca aacaccttgg ttcccagaca ctgtccggca agcatccggt
     3601 ttggtcgccg gatgggtcgt caatcatctt catcaacaac gtcgatggca gcatctggac
     3661 gatgaacgcg gatgggagcg accagactga actcctcgcc tgtgcggatt tgtgcgacga
     3721 gatggactat ccgtcctggt caccagatgg cagcaaagtt gtattcctgg cgtactctga
     3781 cgagtccgct ggtccgccgg cccgatccag cctgatgacg ctcgatctga agtcaggtga
     3841 cctgaccaca cttgcggatg ccaccctccc gcggatcctc gacaccccgc agtggtcgcc
     3901 ggacggtcaa accatcgtcc tggccatgaa tgagctagac gacgagttca accaaaccgg
     3961 cgcggcattg gcgacgatgc cagcgtcagg cggagaactc acctggctga ccgagttctc
     4021 cctctacgcc tactacccaa cctggaacgc cgttaccaat cagatcgtct tcggccagga
     4081 accggtgctt tggtccggcg cctccgatgc cgatacgcag cccctcaatc tctgggtgat
     4141 caatccggat ggcgccgggc ttgaggcgat caccgatctg gccgaaggcg aggcactggt
     4201 gcaatccgcg tggacccccg atggtacgcg catcatcgcc acgctcatgg atcgcaacgg
     4261 accccgcctg gtgtgggttg acccggcctc gggcgagatc acaaagatgc cgacgaccgc
     4321 cgggaatctc tatcctcgcc tgcaacccgt gggcaagagc ccggactgaa cgcacggtcc
     4381 gggaaccggt gctcccgaac gacgaaactc cgggtgacgt gatgcttgcc ggccaagggt
     4441 gcgtctccgt gattgacgga ttccagaacc ggccgatcaa gtaccgcctg tcgcaagccc
     4501 tcgacatgcc ggatcggtgc caactgcctc cgcatcgatc aagccaacgg gtccgcagtg
     4561 t
//
LOCUS       DASSGX010000213         2093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_149508, whole genome shotgun sequence.
ACCESSION   DASSGX010000213 DASSGX010000000
VERSION     DASSGX010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149508"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VFI12_03730"
     CDS             <1..273
                     /locus_tag="VFI12_03730"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HET7055545.1"
                     /translation="IPVGPSYQVPLARAVKRAVGIPVVAVGLITDPQQAEAIVGTGDA
                     DLVALARGILYDPRWPWHAAAALGAKVKAPPQYLRCQPRLHRDLFD"
     gene            428..1405
                     /gene="cyoA"
                     /locus_tag="VFI12_03735"
     CDS             428..1405
                     /gene="cyoA"
                     /locus_tag="VFI12_03735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009486 - cytochrome bo3 ubiquinol oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol oxidase subunit II"
                     /protein_id="HET7055546.1"
                     /translation="MRRRFHPEAALQFGVLAALLPLLGACNRGILDPVGPVGSAEKQI
                     LINSTAIMLAIIIPTMIATVAVAWWFRRGNAKATYRPDWEYSGAIELVVWAIPILTIM
                     LLGGIAWISSHRLDPARPLDSKVPPIKVQVVSLDWKWLFIYPDQGIATVNQLVVPAGT
                     PVNFQLTSATVWNVFFVPQMGSMIYTMPNMTTRLNLQADRTGVFEGRSAHFSGDGFPG
                     MEFNVYSVPRQQFAAWAAGAHAKPPLDARSYKLLERPSSYVKPMIFGNVAPGLFEAIA
                     MGRPPSAQQQQPHNLPSPGASTAQPNSPLEGPSPASQPPAKVPAPGARK"
     gene            1483..>2093
                     /locus_tag="VFI12_03740"
     CDS             1483..>2093
                     /locus_tag="VFI12_03740"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HET7055547.1"
                     /translation="MIALAAVAVWITRIKAWPFLWNEYITSTDHKKIGIMYIVLALVM
                     LVRGFADAIMMRAHQFLAIGPSQGFLTPEHYNQIFSAHGVIMIFFVAMPLVTGLMNYV
                     VPLQIGARDVAFPFLNNFSFWMTAFGGVLVMISLFVGEFARTGWLAYPPLSDILHSPD
                     VGVDYYLWSLQIAGVGTTLSGINLIATIVKMRAPGMTMMKMPV"
BASE COUNT          352 a          709 c          627 g          405 t
ORIGIN      
        1 atcccggtcg ggccgagcta ccaggtgccg ctcgcgcgcg ccgtgaagcg cgccgtcggc
       61 attccggttg ttgcggttgg cctgatcacc gatccgcagc aggccgaagc gatcgtcggc
      121 accggcgatg ccgacctggt cgcgctcgcc cgcggcatcc tctacgaccc gcgctggccc
      181 tggcacgccg ccgccgcact cggcgcgaag gtcaaggcgc cgccgcaata tctgcgttgc
      241 cagccgcgcc tccaccggga tctcttcgac tgaccgcgct gacccacagc ttggaaatcc
      301 gctcgatact ccaagcgagg tcaggacgct cccgcgtgat ggaatgccgc ttcccactga
      361 ctctttgaac atgcgttcag aaagggtacg gggggccatg cactcagcct ggagtcatgg
      421 cgatggcatg cgccgccgct tccatccgga agcggccttg cagttcggcg tcctcgcggc
      481 gctcctcccc ctgctcggcg catgtaatcg cggaatcctt gatccggtcg gacccgtcgg
      541 aagcgccgaa aagcagatcc tgatcaattc gacggcgatc atgctcgcga tcatcatccc
      601 gacgatgatc gccacggtcg ccgtcgcctg gtggttccgt cgcggcaatg cgaaggctac
      661 gtatcgcccc gactgggaat attccggcgc gattgagctt gtggtgtggg caatcccgat
      721 cctcaccatc atgctgctgg gcggaattgc ctggatcagc agccaccggc tcgatccagc
      781 caggccactc gactccaagg tgccgccgat caaggtccag gtggtctcgc tcgactggaa
      841 gtggctcttc atctaccccg atcaagggat cgcgaccgtc aaccagctcg tcgtcccggc
      901 gggcactcca gtcaattttc agctcacttc cgcaacggtc tggaacgtct tcttcgtgcc
      961 gcagatgggc agcatgatct acacgatgcc gaacatgacg acccgcctga acctgcaggc
     1021 ggaccgcacg ggcgtgtttg agggccgctc cgcgcatttc agcggcgacg gttttccggg
     1081 catggagttc aacgtctatt cggttcctcg ccagcagttc gcggcgtggg cggcgggtgc
     1141 gcacgcgaag ccgccgctgg atgcgcgctc ctacaagttg ctcgaacgcc cgagcagtta
     1201 tgtgaagccg atgatcttcg gcaatgtcgc tccgggtctg ttcgaagcca tcgcaatggg
     1261 ccgaccgccg tcggcccagc agcagcagcc gcacaacctg ccctcgccgg gcgcatccac
     1321 ggcgcagccg aacagcccgc tcgagggacc ctcgcctgca agtcagccgc cggcgaaagt
     1381 tccggctcct ggagcccgca aatgagtccg atttttggaa agctgacctg gcaagccatt
     1441 cccttccagg aaccgatccc gctcatcact tcgatcgtgg tgttgatcgc gctcgccgcg
     1501 gtcgccgtgt ggatcaccag gatcaaggcc tggccgttcc tgtggaacga gtacatcacc
     1561 tcgaccgatc acaagaagat cgggatcatg tacatcgtgc tcgccctcgt catgctggtg
     1621 cggggcttcg ccgacgcgat catgatgcga gcccaccagt tcctcgcgat cgggccgagc
     1681 cagggctttc tgacgcccga gcattacaac cagattttct ccgctcacgg cgtgatcatg
     1741 atcttcttcg tggcgatgcc gctggtgacc ggcctgatga actacgtggt gccgctgcag
     1801 atcggcgcgc gcgacgtggc cttcccgttc ctcaacaact tcagcttctg gatgaccgcg
     1861 ttcggcggcg tgctggtgat gatctcgctg ttcgtcggcg aattcgcacg taccggctgg
     1921 ctcgcctatc cgccgctgtc cgacatcctg cacagcccgg atgtcggcgt tgactactac
     1981 ctgtggtcat tgcagatcgc cggggtgggc accacgctct cgggcatcaa cctgatcgcc
     2041 accatcgtga agatgcgcgc gccgggcatg accatgatga agatgcccgt ctt
//
LOCUS       DASSGX010000214         2473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1566494, whole genome shotgun sequence.
ACCESSION   DASSGX010000214 DASSGX010000000
VERSION     DASSGX010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1566494"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="VFI12_03745"
     CDS             complement(<1..845)
                     /locus_tag="VFI12_03745"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7055548.1"
                     /translation="MTHALTRILTLFTKDLKDAIRDARVLVALIVPLGIGVFYNYSFS
                     DTSITTINGTVAIAAADATTLPELITAAVPGNVKLKFVTEPNQAAVERAVSNEDADVG
                     LVVPAGFDQAVQQGTQPALTVVRSPKPSIPGDYVLTTLEPVLRQMADQPFPVAINVTD
                     AAAAKSETLFDKIGMRTWALSLAVVLMLAMISALAIPIVLAEEFEKKTIDALALAMPY
                     REVIIAKALLGLFYMVVMIGVFLGLTRLNVHNWGMFVLGVGLTGLASLGLGLLLAGLL
                     KNPNQ"
     gene            complement(842..1693)
                     /locus_tag="VFI12_03750"
     CDS             complement(842..1693)
                     /locus_tag="VFI12_03750"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7055549.1"
                     /translation="MTDIVVVEDLAKHYGHVRAVDGISFTVAQGEIFGLLGHNGAGKT
                     TTIRMLTGRTRPTAGHASIAGFDVVEQIDEIKPIINLVFEDANLYERLTGRDNLAMFA
                     RLYGVPVARADELLALLGLTEAANRRVKTYSTGMRQRLIIARALINSPRVLFLDEPTR
                     GLDPSSARELRELIVTLAGRGTTVVLTTHYMNEADEMCDRVAFLKAGKIVALDSPREL
                     KLKYGRRAARVLLDNREEREIDLTSPADARALSDWMSGGRVMTLHSEEGTLEDVFINI
                     AGPATSR"
     gene            1812..2042
                     /locus_tag="VFI12_03755"
     CDS             1812..2042
                     /locus_tag="VFI12_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055550.1"
                     /translation="MLAILLVTIGSVSAGTGFMLIGVSKSEHGQNDLMTTVSWFMIIV
                     GVIAAVIGSFWYRANEARLDEERHRADALTQR"
     gene            complement(2057..>2473)
                     /locus_tag="VFI12_03760"
     CDS             complement(2057..>2473)
                     /locus_tag="VFI12_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055551.1"
                     /translation="GAHNTQKMAAVIPDVERLPRPRVGVIGFLAAKRSDEMIALLGQA
                     LDHIVVARPDVLGKPGREVAQTAESVRRRVAIPVVEARDPVEAVRMAEAIAGTTGSVV
                     VTGSLYLCGAVRERWYSSREIVEQQTQWPGITPRPS"
BASE COUNT          437 a          767 c          822 g          447 t
ORIGIN      
        1 aactggttcg gattcttcag tagtcccgcc agcaggagtc cgaggccgag cgaggccaaa
       61 ccggtcaacc cgacgccgag cacgaacatg ccccaattgt gcacgttcag gcgggtcaag
      121 ccgagaaaga caccaatcat gacgaccatg tagaaaagac cgagaagcgc tttggcgatg
      181 atcacctcgc gatacggcat cgcgagggcg agggcgtcga tcgttttctt ctcgaactcc
      241 tcggccagca cgatcgggat cgccagggcg gagatcatgg ccagcatgag gacgaccgcc
      301 agggagagcg cccaggttcg catgccgatc ttgtcgaaga gcgtctccga tttcgccgca
      361 gccgcatcgg tgacattgat cgcgaccgga aacggctggt cggccatttg ccgcaagacc
      421 ggttcgaggg tcgtaagcac gtaatcgccc gggatagacg gctttggtga tcggacgacg
      481 gtcaaagccg gttgcgttcc ttgctgtacc gcctggtcga acccggctgg cacgacgagt
      541 cccacgtcgg cgtcttcgtt gctgactgcc cgctccaccg ccgcctgatt cggctcggtc
      601 acgaatttga gcttgacatt gcccggaacc gcggccgtga tcagttccgg caatgtggtc
      661 gcatcggcgg cggcgatggc gaccgtgcca ttgatcgtgg tgatcgacgt gtcggagaag
      721 ctgtagttgt aaaagacgcc gatgccgagc gggacgatca gcgccacgag cacgcgggca
      781 tcgcggatcg cgtccttcag gtctttggta aagagcgtga ggatccgggt cagggcgtgg
      841 gtcatctgct ggtcgccggt cctgcgatgt tgatgaagac gtcctcgagc gtgccctctt
      901 ccgagtgcag cgtcatgacc cggccgcccg acatccagtc gctgagcgcg cgggcgtcgg
      961 ccggcgaggt caggtcgatt tcgcgctcct cgcggttgtc gaggaggaca cgagcggcgc
     1021 ggcggccgta tttgagcttg agctcgcgcg gcgaatcgag ggcgacgatc ttgccggcct
     1081 tcaggaaggc aacgcggtcg cacatctcgt cggcctcgtt catgtagtgc gtcgtcagga
     1141 caaccgtggt gccccggccg gcgagtgtga cgatcagctc ccggagctcg cgcgcggagg
     1201 acggatcgag accccgcgtc ggctcgtcga ggaagagcac gcgcggcgag ttgatcaacg
     1261 cccgggcaat gatcaggcgc tgcctcatgc cggtcgaata ggtcttgacc cggcggtttg
     1321 ccgcctccgt caggccgagc aaggccagca attcatccgc gcgagccacc ggcacgccgt
     1381 agagccgggc gaacatcgcg agattgtccc gcccggtcag ccgctcatag aggttggcgt
     1441 cctcaaagac gaggttgatg atcggcttga tttcatcgat ctgctcgacg acgtcgaatc
     1501 cggcgatcga agcgtggccg gcggtcgggc gagtgcggcc ggtgagcatc cggatcgtcg
     1561 tggtcttgcc ggcgccgttg tgaccgagga ggccgaagat ctccccctgc gcgacggtga
     1621 acgagatgcc gtcgaccgcg cgaacgtggc catagtgctt cgcgaggtct tcaacgacga
     1681 cgatgtcggt catatgctcc gggggtgttg cgttggcggc aacggtgcgc gttcacaccg
     1741 gccgtaaacg gtacactacc agcgtttgga gatggcgatt cggcataaca acctgaggag
     1801 cgcgttcgat catgctggca atccttctcg tcaccatcgg atctgtctcg gcagggaccg
     1861 gcttcatgct gatcggggtc tcgaagtcgg agcacggtca aaatgacctg atgacgaccg
     1921 tgtcgtggtt catgatcatt gtcggggtga tcgccgccgt gatcggctcg ttctggtatc
     1981 gcgccaacga agcgcggctc gacgaggagc ggcatcgcgc ggatgccctc acccagcgct
     2041 aatcacagca cgtgcctcag gatggccggg gagtgatccc cggccattgt gtttgctgct
     2101 cgacgatctc ccggctcgag taccagcgct cccggaccgc gccgcagaga tagagcgagc
     2161 cggtcaccac gaccgaaccg gttgtgccgg cgatcgcctc cgccatccgg accgcttcga
     2221 ctggatcgcg ggcttctacg accggaatcg caacccggcg acgaaccgat tccgccgtct
     2281 gcgccacctc ccgccccggc ttgccgagta catcgggtcg cgcgacaacg atatgatcga
     2341 gggcctgccc cagcagggcg atcatctcgt ccgagcgctt ggcggccagg aagccgatca
     2401 cgccgacgcg cgggcgcggc aaccgttcga cgtcgggtat gacggccgcc atcttctgcg
     2461 tgttgtgcgc tcc
//
LOCUS       DASSGX010000215         4647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1348371, whole genome shotgun sequence.
ACCESSION   DASSGX010000215 DASSGX010000000
VERSION     DASSGX010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4647
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1348371"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..1543)
                     /gene="ngcE"
                     /locus_tag="VFI12_03765"
     CDS             complement(86..1543)
                     /gene="ngcE"
                     /locus_tag="VFI12_03765"
                     /inference="COORDINATES: protein motif:HMM:TIGR03851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048029 - monosaccharide binding [Evidence
                     IEA];
                     GO_function: GO:0048030 - disaccharide binding [Evidence
                     IEA];
                     GO_process: GO:0006032 - chitin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0015764 - N-acetylglucosamine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine/diacetylchitobiose ABC
                     transporter substrate-binding protein"
                     /protein_id="HET7055552.1"
                     /translation="MPTQDQIQQLIADANSGQHSRRDLLRRAAAMGLSFPAAVALFTG
                     AMPLSALAQDASPAPGATNPLGVDPAAPLDVVIFKGGFGDDYAINVNQNLYNKLYPDA
                     KITYLGTQGLGAQLQPRFVGGNPPDVIDNSGAGNLDTAALVAENQLADLADLMSAASY
                     DTEGKTFADTLVPGSQADGVFDGKQLYLNWALTVIGVWYSSSWMESKGYTYPKTWNDF
                     IALCEEIKGTGVAPFGTTGVHPQYIKNFMFDQMLYKHDPDAAIAIDNLEDDAWKSQAV
                     TDVLTALYQLAEKDLFLSGWEALDHTQSQAEWLQGKAAFLPCGTWLENEMKGLVPDGF
                     DMVVAPTPSLDGDKLPFEAIIATAGEPFIVPAQGKNVQGGKEWLRLLFSKEGGRFFAE
                     NTKSLSVVQGSGEGLDLGSAFASTQEAIANAGDNAFPTARYQSWYKDLQDESRFQLLA
                     LMQKQISIEEFQSTVQDMADQVKDDDSVVKYTREK"
     gene            1997..2512
                     /locus_tag="VFI12_03770"
     CDS             1997..2512
                     /locus_tag="VFI12_03770"
                     /inference="COORDINATES: protein motif:HMM:NF019445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LecA/PA-IL family lectin"
                     /protein_id="HET7055553.1"
                     /translation="MKIDQLHTYQGTSLSRRVIVRAGAKLAYAAPIIAASMQMGGRGV
                     AALSGGQSFTVPANAEAGINTGIAVAQDQEVCVSASGTVQLCTNDNGRNFCPTGPAGD
                     NDVCSPYPVPCCGDFVCGLLLGEVNGIRFALGAGGCATAPASGTLSLVVSDCPGCFGD
                     NDGSYTVTITV"
     gene            2815..>4647
                     /locus_tag="VFI12_03775"
     CDS             2815..>4647
                     /locus_tag="VFI12_03775"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET7055554.1"
                     /translation="MSLFSPASLARASARHPWRVLGAWIVILVLAVMSASGLGDALTT
                     TSNFTGKPESQAGADLLKDRMRGDKPATETIIVRSETMTVDDPGFQAIVEQTSSDVLA
                     LSGVVASAPTYYEAVANGDTSAGHMVSADRHATIIPVTFAGTMDEVDNHVDAYLATVK
                     QHASANVQVMTVGELSVSDEFNTISSHDLEKGEMFSLPLTILILVVVFGALLAVGVPL
                     VLAIASIILALGLTAVVGRFTDLSFYVINMITMIGLAVGIDYTLFVVSRYREERQRGH
                     AKLDAIEIAGGTATKAVVFSGGTVVLALMGMFLVPLTVFHSLGAGAVLAAVSAVAGTL
                     TLVPAMLGLLGDKIDWPRKRRYDDASVRAAYQAHANETYHAGFWGRITRLVMARPAVF
                     TVLSIGLLIALALPYVDIHRGQAGVETLPASDVKTAYEILGRDFSVGLADPVEIVVDG
                     EINDPAIQASIAALQQALAANPDFGTSTVQVNRTGDLALITTPLGLPSDSEAGTAAIW
                     NLRNTVIPQAFSTRAHASVYVSGGPGFNTDYFHIVDSSTPTVFGFVLGLSFLLLLLAF
                     RSIVIAAKAIVMNLLSVGAAYGLMVLVFQEGVGGELFGFQHTPTI"
BASE COUNT          783 a         1472 c         1525 g          867 t
ORIGIN      
        1 ggggaggcgg cacacgtgcc gggcagaaaa atggtccgat tcgccaggcg ggtgacctgg
       61 cgaaccggac cggtcgcgac gaaggttact tctcgcgtgt gtacttcacg accgagtcgt
      121 cgtccttgac ctggtcggcc atgtcctgga ccgtgctctg gaactcttcg atcgagatct
      181 gcttctgcat cagcgcaagg agctggaagc gggactcgtc ctgaaggtcc ttgtaccagg
      241 actggtagcg cgcggtcggg aaggcgttgt cgcccgcgtt cgcgatcgct tcctgggtcg
      301 aggcgaaggc agagccgaga tcgagcccct cgcctgagcc ctggacaacg gagagcgact
      361 tggtgttctc ggcgaagaag cggccgcctt cctttgagaa gaggaggcgc aaccattcct
      421 tgccgccctg gacgttcttg ccctgggctg gaacgatgaa cggctcgccg gcggtggcga
      481 tgatcgcctc gaacggcagc ttgtcaccat cgagcgacgg cgtcggcgcg acgaccatgt
      541 cgaagccgtc cggaaccagg cccttcatct cgttctcgag ccaggtgccg caggggagga
      601 acgccgcctt gccctggagc cactcggcct gcgactgcgt gtgatcgagc gcttcccagc
      661 cggacaggaa gaggtccttc tcagcgagct ggtagagcgc ggtcagtacg tcggtcacgg
      721 cctgcgactt ccaggcatcg tcctcgaggt tgtcgatcgc gatggcggca tccggatcgt
      781 gcttgtacag catctggtcg aacatgaagt tcttgatgta ctgcgggtgg acacccgtcg
      841 tgccgaacgg cgcgacgccg gtgcccttga tctcctcgca gagagcaatg aagtcgttcc
      901 aggtcttcgg gtaggtgtag cccttggact ccatccagct cgagctgtac cagacgccga
      961 tgacggtaag cgcccagttc aggtagagct gcttgccatc gaagacaccg tcggcctgtg
     1021 agccgggaac gagcgtgtcg gcaaaggtct tgccctcggt gtcgtacgac gccgcggaca
     1081 tcaggtcggc cagatcggcg agctgattct cggcgacgag cgccgcggtg tcgaggttgc
     1141 cggcgcccga gttgtcgatc acgtccggcg ggttgccgcc gacgaaccgc ggctggagct
     1201 gggcgccgag gccctgcgtg ccgaggtagg tgatcttcgc gtccgggtag agcttgttgt
     1261 agaggttctg gttgacattg atcgcgtagt cgtcgccgaa gccacccttg aagatgacga
     1321 cgtcgagcgg cgcggccgga tcaaccccga gcgggttggt ggcgcccggt gccggggagg
     1381 cgtcctgggc gagcgcgctg agcggcatgg cgccggtgaa gagcgcgacg gcggccggga
     1441 acgaaaggcc catggcggcg gcgcggcgca gaagatcgcg ccggctgtgc tggccgctat
     1501 tggcgtcggc aatcaattgc tgaatctggt cctgggtcgg cattgaaccc tcctgattgt
     1561 acgccgggcc agtcatcggc cgggcgcgct caaaatcggt gatgcatccg cgttggccac
     1621 gagcggcgcg gtgcgggcat cacaggcaga cgtgcgattg tgctccttcc ggccgcattc
     1681 ctccttatat cctcgaatgg accctgaggt caggcggcga agcgatcgcg gagtggcctc
     1741 aggaaagcac gcagcaaggt atcgactaaa gcgaccggaa ttttccgtcg ccaatcacga
     1801 acccataaac tgtagctcac ccggcgatcc ggttgccaac tgattcaggc atggtcagtg
     1861 cagtaatttc ggtttggtgt gtacgtacag ttgaccaatc tggcacaggc aagtagtttg
     1921 gaatcgtcat ccgccaatat gaaagacgga gatcgagtta ccggctgacg ctgctgacat
     1981 tgggaaggac ccgtcggtga agatcgatca attgcacacg taccagggaa ccagcctttc
     2041 gcgacgagtc atcgtccggg ccggcgcgaa gctggcctac gccgcgccga tcattgccgc
     2101 ctcgatgcag atgggcggac gcggagtcgc tgcgctgagc ggtggccaga gctttacggt
     2161 cccggccaat gccgaggccg gcatcaatac cggcattgcc gtcgcccagg accaggaggt
     2221 ctgtgtcagc gcgtcgggga ccgtgcagtt gtgtacgaac gacaacggcc ggaacttctg
     2281 cccgactggg ccggccggag acaatgacgt ctgttctcca tatcccgtcc cgtgctgcgg
     2341 cgatttcgtc tgcggtctgc ttctgggcga ggtcaatggg atcaggttcg ccctcggcgc
     2401 gggcgggtgc gcgaccgcgc cggcgagcgg aaccctgtcc ctggtggtca gcgactgccc
     2461 aggctgcttc ggcgacaacg acggctccta caccgtgaca ataaccgtat aggcgaaccc
     2521 caacgtagtt ccaatctgcc gtaaacggcc ccggtgatct ctcgccgggg ccgttttgcg
     2581 ttccgcgtgg gtattcccag gacccgtcct gagcgattgc tgtccgccgg acgcgcgtta
     2641 cgggaagctc cgccctttgg cggagttggg aggtccgccg gcggtggggg gtgatggtcc
     2701 gtcacaggtc cgccctttgc ttcgtccgcc cgtacgacga cgctgcgccg ccccgggagc
     2761 agaatcgcag cagtcgattg tgacagggaa cgcagacagc aaggagacgg aaccatgtcg
     2821 ctcttttcac cggccagcct ggcacgggcc agcgcacgtc acccatggcg cgttctcggc
     2881 gcctggatcg tcatcctcgt cctcgccgtg atgtcggcct ccggcctcgg agatgcactg
     2941 accacgacca gcaacttcac cggcaagccg gaatcacaag cgggcgcgga cctgctcaag
     3001 gaccggatgc gcggcgacaa gcccgcgacc gagacgatca tcgtccgctc cgagaccatg
     3061 acggtcgacg atccgggttt ccaggccatc gtcgagcaga cgtcgtcaga cgtgctcgcg
     3121 ctgtcgggcg tagttgcctc ggctccaacc tattacgagg ccgtggccaa tggcgacacg
     3181 agtgccggcc acatggtttc cgccgatcgc cacgcaacca tcatcccggt gaccttcgcc
     3241 ggcacgatgg acgaagtcga caaccacgtc gatgcgtatc tggcgacggt caagcagcat
     3301 gcgtctgcga atgttcaggt aatgaccgtc ggcgagctga gtgtcagtga cgagttcaac
     3361 accatctcgt cccatgacct cgaaaagggc gagatgttca gccttccgct cacgatcctg
     3421 atcctggtcg tcgtcttcgg cgccctgctc gcggtcggtg tgccgctggt actggcgatc
     3481 gcctcgatca tcctcgcgct cggactgacg gcggtggttg gccgcttcac cgacctttcc
     3541 ttctacgtca tcaacatgat cacgatgatc ggcctcgcgg tcggtatcga ctacaccctg
     3601 ttcgtcgtct cgcgatatcg cgaagagcgg caacgcggcc atgccaagct cgatgcgatc
     3661 gagatcgccg gcggcaccgc gaccaaggcc gtcgtcttct cgggcggcac cgtcgtcctc
     3721 gcgctgatgg gcatgttcct ggtcccgctg accgttttcc acagcctggg cgccggtgcg
     3781 gtgctggctg cggtctcggc cgtggccggg acgctgacgc tggttccggc catgctcggc
     3841 ctcctcggtg acaagatcga ctggccgcgg aagcgccgct acgacgatgc gtcggtgcgg
     3901 gctgcgtacc aggcccatgc gaacgagacc taccatgccg gcttctgggg acggatcacc
     3961 cgcctcgtga tggctcggcc ggccgtgttc accgtgctct cgatcggcct gctcatcgcc
     4021 ctggccctgc cgtatgtgga catccaccgt ggacaggcgg gcgtcgagac gctgccggcc
     4081 agcgatgtca agaccgccta cgagatcctc ggtcgcgact tctctgtcgg tctggccgac
     4141 ccggtcgaga tcgtggttga tggcgaaatc aacgacccgg ccatccaggc gtcgatcgcg
     4201 gccctgcaac aggcgctggc ggcgaatccc gacttcggca cgagcacggt tcaggtgaac
     4261 cggaccggcg atctggctct gatcacgacg ccgctcggcc tcccgagcga ctccgaggcg
     4321 ggaacggcgg caatctggaa cctgcgcaac acggtgattc cgcaggcgtt ctcgacccgc
     4381 gcccacgcct ccgtctacgt cagcggcggt ccaggcttca acacggacta cttccacatc
     4441 gtcgatagct cgacgccgac ggtcttcgga ttcgtgctcg ggctgagctt cctgctcctg
     4501 ctgctggcct tccgctcgat cgtgatcgcg gccaaggcga tcgtgatgaa cctgctctcg
     4561 gtcggcgcgg cctacgggct gatggtgctc gtattccagg aaggggtcgg cggagaactc
     4621 ttcggattcc agcacacacc gacgatc
//
LOCUS       DASSGX010000216         2677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1020655, whole genome shotgun sequence.
ACCESSION   DASSGX010000216 DASSGX010000000
VERSION     DASSGX010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020655"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..433
                     /locus_tag="VFI12_03780"
     CDS             218..433
                     /locus_tag="VFI12_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055555.1"
                     /translation="MTNRVRYYITESDDRPIVRSVPVTVSTQPANDLMVIEKQQTEPA
                     AREQATARRPVGRIVKVLRKPSRPTRS"
     gene            449..1822
                     /gene="mgtE"
                     /locus_tag="VFI12_03785"
     CDS             449..1822
                     /gene="mgtE"
                     /locus_tag="VFI12_03785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HET7055556.1"
                     /translation="MDGALQQSTASPEANLDRLREAIDQQDSLLARALARQLLDEDAA
                     ELFEGLDRTEIRALAALLGDETLADLLSQLDEHDAADILEQLPIQHAADVLEEINPDD
                     AADIIVQVDPDVSEDILVAMEPADAAEIRELMAYRPDTAGGIMTPEFVAMEPGLRADR
                     AIAALRKVAEAAETIYYVYITDAEEHLLGVLSLYKLVLSRPDALVEQLMFPDPVRVNV
                     NADQEYVARLLTERNFLAIPVVDDENRLVGIVTADDVADVLEDEATEDIERLGGSAPL
                     SEPYLRASPVLLFRKRIAWLLVLFMAQFVTLEVYDHYQGILEEQLILAMFVPILIGTG
                     GNVGSQTVTTIIRAMAVGEAAPRHFVRILGKEMVTSLGLGLVMGTLMFARGAIQDGRG
                     FGLTVGLTVGVLIIWAATVGAMLPLILSKLRVDPAVVSAPFISSLIDATGLIIYFSMA
                     GLILHVG"
     gene            1897..2304
                     /locus_tag="VFI12_03790"
     CDS             1897..2304
                     /locus_tag="VFI12_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055557.1"
                     /translation="MPDTLLALVPRARLSSMLTAFHRNGYGHVTRVLDPERAPLAQQL
                     GRAGVRASRIAETEPVDSVLLFVHAPKRTTEAAALAIANGATDAEAVACGEPVSAAVA
                     PGLITFAGNRRGRRTERRTPRTSDATPELAADG"
BASE COUNT          464 a          879 c          863 g          471 t
ORIGIN      
        1 ccgatgttcg tcaagaaccg gccggcgcac caaggcccag cgaagggagc ggcgttgaac
       61 gccggtagca gtcctagtca ctcgaccaag gcgcgccctc ttgcctcaga ctgggttccc
      121 gtgagctaag gctccttaac ccatcgcctg acagcaatcc gggggacgcg ccgaactcgg
      181 cacctcggat gtattctgtc ctgaaaggcg gtggcccatg accaaccgtg tccgctacta
      241 catcacggag agcgatgatc gcccgatcgt gcgcagcgtg cctgtgactg tcagcacgca
      301 gcccgcgaat gacctcatgg tcatcgagaa gcagcagact gagccagccg cgcgcgagca
      361 ggcaacggct cgtcgtcccg ttggccgcat tgtcaaagtg ctgcggaagc cgtcccgccc
      421 gactcgctcc taacccggcc gagacctcgt ggatggggca ctgcagcaat cgacggcatc
      481 ccctgaggca aacctcgacc gcctgcgcga ggcgatagac cagcaggaca gcctgctggc
      541 gcgggcgctc gcccgccaat tgctcgatga agatgccgcc gagctcttcg agggcctgga
      601 tcgaaccgag atccgggccc tcgcggcgtt gctcggcgac gagaccctag ccgacctgct
      661 cagccaactt gacgagcacg acgcggccga tatcctcgaa cagcttccga tccagcacgc
      721 cgccgacgtc ctcgaggaga tcaaccccga cgacgcggcc gacatcatcg tccaggttga
      781 tcccgatgtc tccgaggaca tcctggtcgc aatggaacca gccgacgccg ccgagatccg
      841 cgagctgatg gcataccgcc ccgataccgc gggcggcatc atgaccccgg agttcgtggc
      901 gatggagccg gggttacggg cggatcgggc gatcgcggcg ctccgcaagg tggctgaagc
      961 agccgagacg atctactacg tctacatcac tgacgcggag gagcacctgc tcggtgtcct
     1021 gtcgctctat aagctcgtgc tcagccggcc cgacgcactt gtcgagcagc tcatgttccc
     1081 ggatccggtg cgggtcaacg tcaatgccga ccaggaatac gtcgcccggc tgctgaccga
     1141 gcgcaacttt ctcgccattc cggtcgttga cgacgagaac cggctggttg gcattgtcac
     1201 cgccgatgac gttgccgacg tgcttgagga cgaagcgacc gaagacatcg agcggctggg
     1261 cggctccgcc ccgctatccg agccctacct gcgcgcatcg ccggtcctgc tgtttcgaaa
     1321 gcgcatcgcc tggttgctcg ttttgttcat ggcccagttc gtgacgctcg aggtctacga
     1381 ccactatcag ggcatcctcg aggagcagct catcctggcg atgttcgtgc cgatcctgat
     1441 cggcacgggc ggcaatgtcg gttcgcagac agtcacaacc atcatccgcg cgatggcggt
     1501 tggcgaggcg gcgccgcgcc acttcgttcg gattcttggc aaggagatgg tgacgtccct
     1561 tggcctcggc ctggtgatgg gcacactcat gttcgcgcga ggggcgatcc aggatgggcg
     1621 agggttcggc ctgaccgtcg gcctcacggt cggggtgttg atcatctggg cggcgaccgt
     1681 cggcgcgatg ttgccgctca ttctgagcaa gctacgggtt gacccggccg tcgtatcggc
     1741 gccgttcatc agcagcctga tcgacgcgac cgggctgatc atctacttct cgatggccgg
     1801 gctgatcttg cacgtgggct agacccgtcc gtacagggac ggtatgctgc ggcaggtatt
     1861 tgactctctg tcacgcacgc agccaggatc ccgctcatgc ccgataccct tctcgcgctc
     1921 gtcccccgcg ctcgccttag cagcatgctg accgctttcc atcgcaacgg atatgggcat
     1981 gtcacgcgcg tcctcgatcc cgagcgggcg ccgctcgcgc agcagctggg ccgggcagga
     2041 gtccgggcga gccggatcgc cgagactgaa ccagttgact ccgtgctgct gttcgtgcat
     2101 gccccgaaac ggacgaccga ggcggcggca ctggcgattg cgaatggcgc cacggatgcc
     2161 gaagctgtgg cgtgcgggga gccggtgtcc gctgccgtgg cgccgggctt gatcacgttt
     2221 gccggaaacc ggcgtggacg ccgcaccgag cggcgaaccc cccggacatc cgatgccaca
     2281 ccggagcttg cggccgacgg gtagccaccc gcgacccgaa ccgaatcaga ctccccgacc
     2341 ggatcccgtt cccgccaccg aatcggcgcc ggcgttcggc gggcgattcg acacgcgtat
     2401 gccgaaaaag ccggttgacg ggatttgggc ctgaccctag cataccgaca gtctcatatc
     2461 tcaggcagtg atggctaggt gcgttgccga gcctttcggt gtctgtacag ggtcagggga
     2521 atatcgttga gcgggtacga gttgcgcctc gggcggcttt tcaatggcag ctccgggcga
     2581 tccttcatca cagcctacga tcatgctatt acgctgggcg ttccggaggg cggtgaagac
     2641 gcgcggggga cgctgggcaa gatcatcgcg ggcgggc
//
LOCUS       DASSGX010000217         3544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1020663, whole genome shotgun sequence.
ACCESSION   DASSGX010000217 DASSGX010000000
VERSION     DASSGX010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020663"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VFI12_03795"
     CDS             <1..447
                     /locus_tag="VFI12_03795"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7055558.1"
                     /translation="TSAGKPGSFAADRFERFHRELVDRLLPERRIVLARVTADETLVG
                     IFYGFVEDGVVYHYQWGLPQFEDNRLSPGFVTGYLVMEEARRRGLAELNWLAGDSRYK
                     RDLSNAQRSLIWAEKTLSPWHMVVTGLISLYAKLPDTRKHMIRGRA"
     gene            447..1454
                     /locus_tag="VFI12_03800"
     CDS             447..1454
                     /locus_tag="VFI12_03800"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET7055559.1"
                     /translation="MSKVDVALGAFKLLGGDVVSRSVPFGHGVLVVNYHRIGDANESI
                     FDRGVFSTTEDQFDMQMDFLKRNFDVISPHELPVARRMGKGRYVIVTFDDGYRDNYEK
                     AFPILKRHGLKATFFVPTAFIDKRELSWWDTIAWMIRHSDATELPGGKWFAHPVPLTG
                     ENREHAIYEALQKYKALPGRETANYVADIADATGHSSLPTHLGDDLWMTWNNVRELRL
                     AGMTIGGHTVNHPLLARLTSSEQVQEIRGCHERLTEMTGIPPKAFSYPVGKRDTFTTI
                     TRAILLSVGFEYAFSFYGGYQNLVDIDLLDIKRVNVGRRTTQTVFESMTSLPSVLARF
                     A"
     gene            1977..2951
                     /locus_tag="VFI12_03805"
     CDS             1977..2951
                     /locus_tag="VFI12_03805"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HET7055560.1"
                     /translation="MIHSIRASESAPIATVGIVGAGFMGAQLALHIAVHGYPVAVVDQ
                     SADALTRMRRGHEEELDRRCASGELSAEERAAVLARVRAATDLAESVRDVDLVIEAVP
                     ERLDLKRAVFGELDRICPPRAILATNSSSFRVSLIEDATGRPDRVLNTHFYPPVWQRP
                     MVELMRGTATSDETIGRVRRFARSVGLTPLVVRKESTGFLFNRVWRAIKRETLHLVDE
                     GVATVDDVDRAWMIALGTPIGPFGLMDMTGLDVVLDVERTYHAESGDPRDAPPRLLTD
                     KVAAGELGAKAGRGFYRYPHPAYQDPAWRQGEGDDVSGNVPGTGMADG"
     gene            2944..3369
                     /locus_tag="VFI12_03810"
     CDS             2944..3369
                     /locus_tag="VFI12_03810"
                     /inference="COORDINATES: protein motif:HMM:NF025292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HET7055561.1"
                     /translation="MGENPLVGTWRLVSFAVRDEEGRIVFPFGRDAVGFITYTGDGHM
                     AVQFGRAERAHLAVGDWVGAGPAEIATAAREYFAYCGTYEYRDGEVMHRVELSLMPNW
                     IGGEQMRLVVFDEDTITLATPPMPVGGRQQIATLVWQRA"
BASE COUNT          593 a         1142 c         1210 g          599 t
ORIGIN      
        1 acctcggccg gcaagcccgg ctccttcgcc gccgaccgct tcgagcgctt ccaccgcgaa
       61 ctggtcgatc ggctgctgcc ggagcggcgg atcgtgctcg cccgggtcac ggccgacgag
      121 acgctggtcg gcatcttcta cggcttcgtc gaggacggcg tggtctacca ctaccagtgg
      181 ggactcccgc agttcgagga caaccgcctt tcccccggct tcgtgaccgg ctacctggtc
      241 atggaggagg cgcggcggcg tggcctggcc gagctcaact ggctggccgg cgacagccga
      301 tacaagcgtg atttgtccaa tgcgcaacgt tcgttgatct gggccgagaa gacgctcagc
      361 ccgtggcata tggtggtgac cggcctgatc tcgctctatg cgaaactgcc tgacaccagg
      421 aagcacatga ttcgaggacg cgcgtgatgt cgaaggttga tgttgccctc ggggcgttca
      481 agttactggg cggggacgtc gtctcccgca gcgtgccgtt cggacacggc gtgctggtcg
      541 tcaactatca ccggatcggc gatgcgaacg agtcgatctt cgatcgcggc gtcttcagca
      601 ccaccgaaga ccagttcgac atgcagatgg acttcctgaa gcggaacttc gacgtgatca
      661 gcccgcacga gctgccggtt gcccgccgga tgggcaaagg tcgctacgtg attgtcacct
      721 tcgacgacgg ctatcgcgac aactatgaga aggcgttccc gatcctgaaa cggcacgggc
      781 taaaggcgac gttcttcgtc ccgaccgcct tcatcgacaa gcgggagctc tcctggtggg
      841 acaccatcgc ctggatgatc cgccacagcg acgcgaccga gttgcccggg ggcaaatggt
      901 tcgcccaccc ggtccccttg accggcgaga accgcgagca cgccatctat gaggcgctcc
      961 agaagtacaa ggcgctgccg ggccgcgaga ccgccaacta cgtggccgac atcgcggacg
     1021 ccaccggcca cagctcgctc ccgacgcatc tcggcgatga cctctggatg acctggaaca
     1081 acgtccgcga gctgcgcctg gccggcatga cgatcggcgg ccacaccgtc aatcacccgc
     1141 tcctggcccg cctgacctcg tcggagcagg tgcaagagat ccgcggctgc cacgagcggc
     1201 tgaccgagat gaccggcatc ccgcccaagg ccttctccta cccggtcggc aagcgggaca
     1261 ccttcacgac gatcacccgc gcgattctgc tctcggtcgg atttgagtac gccttcagct
     1321 tctacggtgg ctaccagaac ctggtcgaca tcgacctgct cgacatcaag cgggtcaacg
     1381 tcggccgtcg caccacccaa accgtcttcg agtcgatgac ttcgctcccg tcggtattgg
     1441 cccgcttcgc ctgagtgaag tcgagcaggc gagcagtcga gcaggcgagc agccgaaaag
     1501 gcgcgggtgc aggcccaaat gtggcgcgtg tgcgtccata ccctattcgg ctctcccgga
     1561 agggagagag catcccgcga tggctgagcc atgatggctc atcagggaga gcgggtcccg
     1621 gttgggagac gggcataccg aagccgcccg gggataaatg cccggggaaa aagacgaagc
     1681 ccgctgaagg ggctgagggg gtgctcgcac gacaacgtag ccccgggcgg ccccgcccgg
     1741 ttgcgctgcc tggaggttcg ttccctgccg ccctcgctgg catcgtcctc gtcttcctgc
     1801 gccctgatcg gcacgcgttt cctttcccgg gcaggtgcgc aacgagcgtg atgaccgcga
     1861 gcagatcagc agcggttgac gcagttttcc ccgggcggga gaatgtccag gattctggga
     1921 aagtcaatgc gactgggcgc ctctcactcg tctgcacccg caggaggtga accgtgatga
     1981 tccactcgat tcgggcgtcg gagtccgcac cgattgcgac cgtgggcatc gtcggcgcgg
     2041 gtttcatggg tgctcagctc gcgctgcaca ttgccgttca cggctacccg gtcgcggtcg
     2101 tcgaccagtc ggcagacgcc ttgacgcgga tgcgccgggg gcacgaggag gaactcgacc
     2161 gccgctgcgc ctcgggcgag ctctccgccg aggagcgggc ggccgtgttg gcacgcgtcc
     2221 gcgcggcgac cgacctcgcg gagagcgtca gggatgtcga cctcgtgatc gaggcggtgc
     2281 cggagcggct cgatctcaag cgggcggtgt tcggtgagct cgatcggatc tgcccgccgc
     2341 gcgcgatcct ggcgacgaat agctcgtcgt ttcgggtctc gctcatcgag gacgcgactg
     2401 ggcggccaga ccgtgtcctg aacacgcact tctacccgcc agtctggcag cggccgatgg
     2461 tcgagttgat gcggggcacc gcgacctcgg acgagacgat cggacgcgtc cggcggttcg
     2521 cgcgatcggt tggcctgaca ccgctggtgg tgcggaagga gagcacgggg ttcctgttca
     2581 accgggtctg gcgggcgatc aagcgggaaa cgctccacct cgtcgacgag ggggtcgcga
     2641 ccgtcgacga cgtcgaccgg gcctggatga tcgccctggg cacaccgatt gggccgttcg
     2701 gcctgatgga catgaccggc ctggacgtcg tactcgacgt ggagcgcacc tatcacgccg
     2761 agtcgggcga tccgcgggac gcgccgcccc ggctgctgac cgacaaggtg gccgccgggg
     2821 aacttggggc gaaggcgggg cgcggctttt accgctaccc gcatccggcc taccaggacc
     2881 cggcctggcg gcagggagaa ggagacgacg tctccgggaa tgtcccaggg acgggaatgg
     2941 ccgatgggtg agaatccgct ggtcgggacg tggcgactcg tgtcgtttgc agtccgggat
     3001 gaggaggggc gcatcgtctt ccccttcggt cgggacgccg tcggcttcat cacctacaca
     3061 ggcgacgggc acatggcggt ccagttcggg cgggcggaac gcgcgcacct cgccgtcggc
     3121 gattgggtgg gggcggggcc cgcagagatc gcgacggcgg cccgagaata tttcgcgtac
     3181 tgcgggacct acgagtatcg cgacggcgag gtgatgcatc gggtggagct gagcctgatg
     3241 cccaactgga tcggcgggga gcaaatgcgg ctcgtcgtgt tcgatgagga caccatcacc
     3301 cttgccacac cgcccatgcc ggtcggcggc cgccagcaaa ttgccaccct cgtctggcaa
     3361 cgggcgtgag gccagggtgt tgtgacacgc tcttggtccc ttcagcggac ttattgtgat
     3421 gcagcccggg ccttgagctc ctggcggcgc tggacggtcc gtcgtctatc cagaacccgt
     3481 cccaggggac tggccacggc cggaaatgcg tttcaggctc cgccgttcat gcgagaatcg
     3541 cggg
//
LOCUS       DASSGX010000218         4115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1457320, whole genome shotgun sequence.
ACCESSION   DASSGX010000218 DASSGX010000000
VERSION     DASSGX010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4115
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1457320"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VFI12_03815"
     CDS             <1..482
                     /locus_tag="VFI12_03815"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HET7055562.1"
                     /translation="GDSEQDRDEQQSINLTELATFVERMVAVAQRAGLASLEIEANGL
                     ELELKFGGTAEANWQVVVPVPPAPAPLANVPAEPEPIGYVINAPMVGTFYHSTSPADP
                     PLVQVGDRVEVGQLIGIIEAMKIMNEITADRGGTVLEVMAGNATAVEYGSPLIRLGP"
     gene            487..1899
                     /gene="xseA"
                     /locus_tag="VFI12_03820"
     CDS             487..1899
                     /gene="xseA"
                     /locus_tag="VFI12_03820"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HET7055563.1"
                     /translation="MQTQVVTVSQVSDYLRLLTESDAFLSNLLVEGEVTDVYLSRAGH
                     LYFTISDGQASLKAVAFRNAAMRVQHFVSVGAHLVVGGRVTVYARDQTCQIYVEFVDH
                     AGMGGQALALARLRAQLEADGLFESERKRPMPVYPHTIGVVTSATGAVIHDIQTVLRR
                     RFPFAHLIVSPAQVQGTGAVESLISALDLVIEDGRAEVVIIARGGGSAEDLSAFNDER
                     LARAVYAAPKPIISAIGHETDVSILDEVADLRAPTPTAAAELITPDIADFALELIDGR
                     DRMDRAFRQILNQNRNDLMQLVQRLDRSSPALILEERRIRLADLESRLTAAVHRHIRD
                     AQSDCRLISVQLHSAGLRVLDRQRIDLAGIQSHLRELPFAHLDAQRSSLAQTERRLAA
                     RAVSVLAGAYADLDVQKSALAKLNPSAVLSRGFALLSTADGAVIGEIGAVRPGDAIRA
                     VVRDGTISADVTATVPAGST"
     gene            1896..2183
                     /gene="xseB"
                     /locus_tag="VFI12_03825"
     CDS             1896..2183
                     /gene="xseB"
                     /locus_tag="VFI12_03825"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HET7055564.1"
                     /translation="MTESANSKQVLDSCDQLSVSEDYEAVRNGLTQVITLLEQPGLGL
                     NDSVRAYEVGRRLAEQCQRLLDAAELRITQLDGAGSDTGTGPLTSRVRLDS"
     gene            2185..3210
                     /locus_tag="VFI12_03830"
     CDS             2185..3210
                     /locus_tag="VFI12_03830"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HET7055565.1"
                     /translation="MNDPRFLPAGKLPGDLLGRLLERHAVRDERVLIGPGTGRDAAAI
                     RFGDCALVVKSDPITFPTDHPAHHLVHINANDIACQGATPKWLLVTALLPHGRATAEM
                     ATELFADLNRCAGEVGVEVIGGHTEITIGLDRPILVGTMIGETAVDRLIDPRNARPGD
                     RLLMTKTAGIEGTAILASDFGPVLCERGIPDQTIATAKSMIFEPGISVLTEARALAEE
                     NAVSAMHDPTEGGIGTALRELAIAAGTGLVASRDAIPVAGTTRAVTDALGVDPLGMLA
                     SGSLLAAVPKERIQAAEDSLARAGVPFAWIGKLTPPQSGMRLRTGATETALPEFTVDE
                     VARLLAG"
     gene            3217..>4115
                     /locus_tag="VFI12_03835"
     CDS             3217..>4115
                     /locus_tag="VFI12_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HET7055566.1"
                     /translation="MTWVLTGSPNADVGFEQGATVLRSGGSAIDAVIATIREVEANPD
                     DHSVGYSGLPNLLGEVELDASIMDGRGLRAGSIGALQNHQDAIELARRVMDELPHVLL
                     AGAGAERFADEQGLPRVDLLTDAAKAVWLQRADLTLQRSKYYERMSELVKATTSDPQF
                     PEPPHGTVNVIARDLAGNIACGVSTSGWAWKYPGRLGDSPIIGAGNYADNRYGAAACT
                     GRGEMAQRCLTAHSVVTFMRFGKSLEAALRIAAEDLRDLDDPYASEMNIVAIDANGNH
                     NAASTAKGKTYIFQTGAMRAAECG"
BASE COUNT          737 a         1328 c         1361 g          689 t
ORIGIN      
        1 tgggcgacag cgagcaggac cgcgacgagc agcaatcgat caatctcacc gaactggcca
       61 catttgtcga acgcatggtc gccgttgccc agcgagccgg actggcgtcg ctcgaaattg
      121 aagcgaacgg cctggagctc gagctcaaat tcggcgggac cgctgaagcg aactggcaag
      181 tcgtggttcc ggttccgccg gcacccgcgc cgctggccaa cgtaccagcc gagccggagc
      241 caatcgggta cgtgatcaat gcgccgatgg ttgggacgtt ctatcactca acctcgccgg
      301 ccgatccgcc gctcgtgcag gtcggcgatc gtgtcgaggt cgggcagttg atcggcatca
      361 tcgaggcgat gaagatcatg aatgagatca cggccgaccg cggcggcact gtgctcgaag
      421 tgatggccgg gaatgcgacc gcggtcgaat atggctcgcc cttgattcga ctcggcccct
      481 agccggatgc aaacccaggt cgtcaccgtc agccaggttt cggattacct ccggctcctg
      541 accgagagcg atgccttcct gagcaacctg ctcgtcgagg gcgaggtgac cgacgtctac
      601 ctgtcccggg ccggccacct ctacttcaca atttccgacg gacaggcatc gctcaaagcg
      661 gttgcgttcc gcaatgctgc catgcgcgtg cagcacttcg tgtccgtcgg ggcgcacctc
      721 gtcgtcggtg gtcgcgttac ggtctacgcc agggaccaga cctgccagat ttatgtcgaa
      781 ttcgtcgacc acgccgggat gggtggccag gcgctcgccc tcgcccggtt gcgcgcccaa
      841 ctcgaggccg atgggttgtt cgaatcggag cggaagcggc cgatgccggt ctatccccat
      901 acgatcggcg tcgttacctc cgctaccggc gcggtcattc acgacatcca gacggttctc
      961 cgccgccgat tcccgttcgc gcacctgatc gtctcgccgg cgcaggtcca gggcacgggc
     1021 gcggtcgaga gcttgatctc ggcgctcgat ctcgtgatcg aggacggccg cgcggaagtc
     1081 gtgatcatcg cgcgaggcgg cggttctgcc gaggacttga gcgcgttcaa cgacgagcgc
     1141 cttgctcgtg cagtttatgc cgccccgaaa ccgatcatca gcgcgatcgg gcacgagacc
     1201 gacgtatcga tcctcgatga agtggccgat cttcgcgccc cgacacccac cgcggcggca
     1261 gagctgatta ccccggatat cgccgatttc gcgctcgagt tgatcgacgg cagagaccgc
     1321 atggaccggg cgttccgcca gatcctgaac cagaaccgca acgacctgat gcagctcgtg
     1381 caacgtctgg accgatcgag ccctgccctg atcctggagg aacgccgaat caggctcgcg
     1441 gatctcgaat cccgtttgac cgcggcggtc caccgccaca ttcgcgacgc ccagtcagac
     1501 tgccggctga tctcggttca gcttcacagc gccgggttgc gggtactgga ccggcagagg
     1561 atcgaccttg ccggcatcca gagccacctg cgcgagctgc ccttcgcgca cctcgacgcg
     1621 caacggtcga gtctcgcaca gaccgagcgc cggcttgccg cgcgcgccgt ttcggtgctc
     1681 gcgggggcgt atgccgatct tgacgtgcag aagtcggcac tggcaaagct caacccgtcg
     1741 gccgtgctga gtcgcggctt cgcgctactc tcgacggcag acggcgccgt gatcggcgag
     1801 atcggcgcgg ttcggccggg cgacgccatc cgggcggtcg tccgggatgg cacaatatcg
     1861 gcagatgtaa ccgcgactgt gccagcagga tccacatgac cgagtccgcc aacagcaaac
     1921 aggtgcttga cagctgcgat cagctttccg tctccgagga ttacgaggcg gtccgaaatg
     1981 gactgacgca ggtgatcacg ctgctcgagc agccgggact tggactgaac gactccgtgc
     2041 gcgcgtacga ggtcggccgc aggctggccg agcaatgcca gcggttgctg gacgccgcgg
     2101 aattgcgaat cacgcaactc gatggtgcgg gcagcgacac cggcacgggg ccgctgacca
     2161 gccgggtcag gctggattcg tgagatgaac gatccccggt tcctgccggc cggtaaactg
     2221 cccggcgatt tgctgggccg cctcctcgag cgccatgccg tgcgcgacga gcgggtattg
     2281 atcggccctg gcaccggtcg ggatgcggcc gcgatccgct tcggtgactg tgcgctggtc
     2341 gtcaagtccg acccgatcac ctttccgacc gatcacccgg cccatcacct cgtccatatc
     2401 aacgcgaacg acatcgcttg tcagggtgcg acgccgaagt ggctgctggt aactgcgcta
     2461 cttcctcatg gccgtgcgac ggctgagatg gcgactgaat tgttcgcgga tttgaaccgg
     2521 tgcgcgggtg aggtcggagt cgaggtcatc ggcggccaca ctgagatcac gatcgggctg
     2581 gaccggccaa tcctcgtcgg cacgatgatc ggcgagacgg ccgtcgaccg ccttatcgat
     2641 ccgcgcaacg cgagacccgg cgaccggctg ctgatgacca agaccgcggg aatcgaaggc
     2701 acggccatcc ttgcctcgga tttcggtcca gtgctttgtg agcgcgggat tccggaccag
     2761 acgatcgcga ccgcgaaatc gatgattttc gagcccggga tttcggtgct gacggaagcg
     2821 cgcgccctgg ccgaggaaaa cgcggtaagc gccatgcatg acccgaccga gggcgggatc
     2881 ggaaccgcac tgcgcgaact ggccattgcg gcggggacgg gcctggtggc cagtcgcgac
     2941 gcgattccgg tggccgggac gacccgggca gtcaccgacg cgctcggcgt cgatccgctc
     3001 gggatgcttg cctccggaag tctgctcgca gcagtcccga aggagagaat ccaggctgcc
     3061 gaggactcgc tcgcgcgcgc gggcgtgccg ttcgcatgga tcggcaagct gacccctccg
     3121 cagagcggga tgcgactccg gaccggcgcg accgagacgg ctttgcccga atttactgtc
     3181 gacgaagttg ctcgcttatt ggcagggtaa gaatcaatga cctgggtgtt gaccggtagc
     3241 ccgaacgccg acgtcgggtt cgagcaggga gcaactgtgc tccgcagcgg tggttcggcc
     3301 atcgacgccg tgattgcgac gatccgcgag gtcgaggcca acccggacga tcacagtgtc
     3361 gggtattccg ggctcccgaa tttgctcggc gaagtcgaat tggacgcaag catcatggac
     3421 gggcgcggac tgcgggccgg atccatcggc gcgctccaga accaccagga cgcaatcgag
     3481 ctcgcgcggc gggtgatgga cgagttgccg cacgtcctgc tggccggcgc cggtgctgag
     3541 cggttcgccg acgagcaagg attgccgcgc gtcgatctgt taaccgacgc ggcgaaagct
     3601 gtctggctcc agcgggccga cctgacgctc caacgctcga agtattacga gcgcatgtcg
     3661 gagctggtca aggcgacgac gagcgatcca caatttcccg agcccccgca cggcacggtc
     3721 aacgtgatcg cgcgagacct cgccggcaat atcgcctgcg gggtaagcac gtcaggttgg
     3781 gcgtggaagt acccgggacg cctcggcgac tcgccgatca tcggcgccgg caactacgcg
     3841 gacaaccggt acggcgcagc agcctgcacc gggcgcgggg aaatggccca gcgctgcctg
     3901 acggcgcact cggtcgtgac cttcatgcga ttcggcaagt ccctggaagc ggcgctgcgg
     3961 atcgcggccg aggacctgcg cgacctcgat gatccctacg caagtgaaat gaatatcgtc
     4021 gctatcgatg cgaacgggaa ccacaacgcg gcctcgaccg ccaagggcaa gacgtacatt
     4081 ttccagaccg gcgcgatgcg cgccgcagaa tgcgg
//
LOCUS       DASSGX010000219         3030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_913427, whole genome shotgun sequence.
ACCESSION   DASSGX010000219 DASSGX010000000
VERSION     DASSGX010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913427"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1816
                     /locus_tag="VFI12_03840"
     CDS             <1..1816
                     /locus_tag="VFI12_03840"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET7055567.1"
                     /translation="AQSDAVRLFLDRARAVRPALELNDETAKAIARICELLDGLPLAI
                     ELAAARANVLSPQAMVSRLERRLPLLTGGARDRPLRQQTMQDAIAWSFDLLTADEQAL
                     FRRLSIFVGSFTLGAAEAVTGAFNQPGIDVLEGIGSLVDKSLLRAWDDDDESPRFAML
                     ETIREYALEQLEQAGEAAATADAHADWFLALAGRADAVSYTPGAPAWYGRLKPDRPNF
                     RAALAWLHAHDPDHRFIELSALLGRFWYKWERFTEGLTWLEAAVAIARRQAPSRTRAE
                     LLDNLGKLTSVRTRHMSPIAYFAESLETWVAVGDARGVARETITLAEGYRLAMDAERA
                     IPLYERGLELISAFPAETSWRSTALRGLATVQLQRGNVDRAEQLFEQSFAVALESDVA
                     WSIGTAHHGLGQIASLRGHHAAAITHFVASIRIMRDLHDRLALLLAIPALGDALVQAA
                     QFERAAHIFGASDAFSETLPDVEGTREMLSCYVPVIATARARLGQAAYSTAWAIGRGQ
                     PVADLVAFALAQAQAALVELAGQVPEPRRPALPGGLSEREAEVLRLAAAGLSNVEMAE
                     RLYLSPHTIRAHLQRIYAKLEVENRAAAVRFAVENGMV"
     gene            1879..2931
                     /locus_tag="VFI12_03845"
     CDS             1879..2931
                     /locus_tag="VFI12_03845"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET7055568.1"
                     /translation="MDTSFPTTDSMMYQAIHRQPQDLRRILADGWADAGQAAGIIAGA
                     SRIWITGIGTSYHAALVGEWLLRSTGKDVRAVTSFDFATYPDQFPLRPSDAVIVQAHT
                     GVKSYSALALQRAVDAGVPVISVGSESAEHPGSQMILRTVEREKSAAYTSSHLCAMAR
                     LAQVAVSLGTDQLREPLMALPDQIAGVIAREGEIEPVARDAANRHIYAIGAGPNEPTA
                     LEFVIKAREAALQQCDGLGLEQFFHGPIVAVNAGDFGVVISMHGASDHRTAAVAKALG
                     MIGLTVWSTGDADPEVPGGSHFALPSTHELISPLLAVVPIQLFAYFLAATRGTHPDRF
                     RREDPVYADAFGQLTL"
BASE COUNT          474 a         1055 c          982 g          519 t
ORIGIN      
        1 cgcgcagagc gatgccgtcc gactgttcct ggaccgcgcc cgggccgtcc gtccggctct
       61 ggagctgaat gacgaaaccg cgaaggcgat cgcccggatc tgcgagctcc tcgatgggtt
      121 gccgctcgcg atcgagctgg ccgccgcccg cgccaacgtc ctgtcgccgc aggcgatggt
      181 cagtcgcctc gaacgccggt tgccgctgtt gaccggtggc gcgcgcgatc gcccgctccg
      241 ccaacagacc atgcaggatg cgatcgcctg gtccttcgac ctgctcaccg ccgacgagca
      301 ggcgctgttc cgccggttgt cgatcttcgt cgggagtttc acgctcgggg cggccgaggc
      361 ggtgaccggc gcgttcaatc agcccggaat tgacgtcctc gaggggatcg gctcgctggt
      421 cgacaagagc ctgctgcgcg cttgggacga tgatgacgaa tcgccgcggt tcgcgatgct
      481 ggaaactatt cgcgagtacg cgctcgaaca gttggagcag gccggcgaag cggcggcaac
      541 ggcggacgcg catgccgact ggttcctggc gctggccgga cgtgccgacg cggtctcata
      601 cacgccagga gcgccggcct ggtacggccg actcaaaccc gaccggccaa actttcgcgc
      661 cgctctcgcc tggttgcacg cgcacgatcc tgaccatcga tttatcgagc tgtccgcgct
      721 ccttgggcgc ttctggtaca agtgggagcg ctttacggaa gggctcacct ggctcgaggc
      781 ggcggtcgca atcgcccggc ggcaggctcc gtcgcgaacc agggccgaac tcctcgataa
      841 cctcgggaag ctgacctcgg ttcgcaccag gcacatgagc cccatcgcct actttgccga
      901 aagcctggag acgtgggttg ccgtcggcga cgcgcgtggc gttgcccgcg agacgattac
      961 ccttgccgag ggctatcgcc tggcgatgga tgccgagcgt gcaattccgc tctatgagcg
     1021 aggattggag ctgatcagcg cgttccccgc cgagacctcc tggcgctcaa ccgcactccg
     1081 gggactcgcc acggtccaac tgcaacgcgg gaacgtcgac cgggccgagc agctcttcga
     1141 gcagtcgttc gccgttgcgc tggagagtga tgtcgcctgg tcgatcggta ctgcccatca
     1201 tgggttgggc cagattgcga gccttcgcgg ccatcacgct gcggcaatca cccatttcgt
     1261 cgcaagcatc cggatcatgc gggatctgca cgaccgcctt gcgctgctcc tcgctattcc
     1321 cgccctcggt gacgcgctcg tccaggcggc tcagttcgag cgagccgcgc acatcttcgg
     1381 ggcaagcgac gccttctcgg aaacgctccc cgacgtggag ggcacgcgag agatgctttc
     1441 gtgctatgtc cccgtcatcg ccacggcgcg cgcacgactg ggacaggcgg cctactccac
     1501 tgcctgggca atcggccgcg gccaaccggt ggcggatctc gtcgcgtttg cgctcgcgca
     1561 agctcaggca gcgctcgtgg aactcgccgg ccaggtaccg gagccccggc gccctgcgct
     1621 ccccggcgga ctctccgagc gcgaagctga ggtcctgcgc ctggctgcgg ccggtctctc
     1681 caatgtcgag atggccgaac gtctctacct cagcccccac acgattcgcg cccatctcca
     1741 gcgcatctat gccaagctgg aggtcgagaa ccgggctgct gccgtccgct tcgccgtcga
     1801 gaacggcatg gtgtagcttg caaaatcggc agacgcaact ttggtcgtgt gccggtccgt
     1861 aattcaatcg gagggcttat ggacacgtcg tttccgacca ccgactcaat gatgtaccaa
     1921 gcgatccatc gccagccgca ggacctgcgc cggattctgg ccgatggctg ggccgatgcc
     1981 gggcaggccg ccgggatcat cgccggtgcg agccggatct ggatcaccgg catcggcacc
     2041 agctaccacg ccgccctcgt cggcgaatgg ctgctccgct cgaccgggaa ggacgtgcgg
     2101 gccgtcacct cattcgattt cgcgacgtat ccggaccagt ttccgcttcg gccgagcgat
     2161 gccgtcattg ttcaggccca caccggcgtc aaaagctact ccgcgctggc cctgcagcgg
     2221 gcggtcgatg ccggcgtgcc ggtcatctcg gtcggcagcg aatcggcaga gcatcccggc
     2281 tcgcagatga tcctgcgcac cgtcgagcgc gagaaatcgg ccgcctacac ctcatcgcac
     2341 ctgtgcgcga tggcgcgcct cgcccaggtc gccgtctcgc tcggcacaga tcagctccgg
     2401 gagccgctga tggccctgcc cgaccagatt gccggcgtga tcgcgcgcga gggcgagatc
     2461 gagccagtcg cgcgggatgc cgccaatcgc cacatctacg cgattggcgc cgggccgaac
     2521 gagccgaccg cgctcgagtt cgtaatcaaa gcgcgggagg ctgccctgca gcaatgcgac
     2581 ggtctcgggc tggagcagtt cttccacggc ccgatcgtcg ccgtgaacgc cggcgacttc
     2641 ggcgtcgtca tctccatgca cggcgcgtcg gaccaccgga cagccgcggt cgcgaaggcg
     2701 ctcggcatga tcgggttgac ggtctggtca actggcgacg cggatcccga agttcccggc
     2761 gggtcgcatt ttgcactgcc atcgacacac gagctgatct cgcctctcct ggctgtggtc
     2821 ccgatccaac tcttcgccta ttttctggcc gcgacccggg gcacgcaccc ggaccgcttc
     2881 cggcgcgagg acccggttta cgccgacgcg ttcggccagc tgacgctgta atgcgacacg
     2941 cagacgttgc tgagtcaatc gggcacgtag cgcggggcgg ctcgccccgc atccgacggc
     3001 caacgcgagg cgagccgccc cgcgctacat
//
LOCUS       DASSGX010000220         2142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1457418, whole genome shotgun sequence.
ACCESSION   DASSGX010000220 DASSGX010000000
VERSION     DASSGX010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1457418"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="VFI12_03850"
     CDS             <1..1080
                     /locus_tag="VFI12_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055569.1"
                     /translation="ATPTSTPNQLATAEVRAARTATVQAENVAALNATVESASDAAQA
                     TEAARATADARLSNMLTATARADATRQAMTSDQQATAAAQATAEAISSEATISAFATQ
                     QAQSDANVDEVTRNLATAAAQATSDALSAEATISAQQTEAAQVEAIATQNAESGANAL
                     ATANAAATQIVEIQAIATQNAESGANDLATAQAQAEAAQATAEALSQSQASSDSDRAT
                     AQAEVANIQATVESVKATATIISQRAGLNSIDQGYAEVTIKVDASGLLAGDKDAENAA
                     KKAVRDALKDYDGCRVGITLTFGWDPDINRGLAIADAVNKLLPDAEPAMFEEAAYENF
                     GNLQPAGTVEIRMYFFRGCQALNND"
     gene            complement(1090..1668)
                     /locus_tag="VFI12_03855"
     CDS             complement(1090..1668)
                     /locus_tag="VFI12_03855"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HET7055570.1"
                     /translation="MGTIVERPLRDALRLIVPGPVVLVSSMSKGQPNVMTVAWVAAQS
                     LSPTLISVAIHPDRLTHQFVSATEEFVINVPVLDWLSTVHEAGMISGRDGDKFVALDL
                     EPADSTVIEPPRITGCPGYIECRVRDRVTAGDHDLFIAEVVAVAADDESFDGFWDVES
                     EAGRLLHHLGADRYAGLAHQYRTTPPETEEEG"
     gene            complement(1670..>2142)
                     /locus_tag="VFI12_03860"
     CDS             complement(1670..>2142)
                     /locus_tag="VFI12_03860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7055571.1"
                     /translation="GDTKLVIGGAPHSANRGVFPPLDPLIAELDIGDRVVLTGRISED
                     DKVALMQGAAVYVTPSLYEGFGLSVLEAMACGIPVIAANRTSFPEIVADNGLLVEPRV
                     EPVAAALQSILTDDALAHRLGSAGLERSRAFDWRRTAEQTVAVYEHVRSNEGQR"
BASE COUNT          414 a          742 c          684 g          302 t
ORIGIN      
        1 gccacgccga cctcgacgcc gaaccaactg gcgacagccg aagtccgcgc cgcgcgcacc
       61 gcgaccgtgc aggccgaaaa cgtggctgcc ctgaacgcga cggtcgaaag cgcgtcggac
      121 gctgcccagg ctaccgaggc cgcccgcgcg accgcagacg cgcgcctctc gaacatgttg
      181 accgcgaccg cgcgggcgga tgcgacccgg caggcaatga cgtccgatca gcaagcgacg
      241 gctgccgccc aggcgaccgc cgaagcgatc tcctccgagg cgacgatctc ggcatttgca
      301 acgcagcagg cgcagagcga cgcgaacgta gacgaggtca cacgaaatct ggcgacggcc
      361 gccgcgcagg ccacctccga tgcgctgtcg gccgaggcaa cgatctcggc acagcagacg
      421 gaagcggccc aggtggaggc gatcgccacc caaaacgccg aatccggcgc gaacgcgctt
      481 gccaccgcga acgcggccgc gacgcagatc gtcgagatcc aggcgatcgc gacccagaat
      541 gccgagtccg gcgcaaacga tctggccacg gcgcaggcgc aagcagaggc cgcccaggca
      601 acagccgagg cgctttctca gtcgcaggcc tcgagcgatt cggaccgggc gacagcccag
      661 gccgaggtcg ccaatatcca ggccaccgtg gagtcggtca aggcgacggc aaccatcatc
      721 tcgcagcggg ccggcctgaa ctcgatcgac cagggctatg ccgaagtcac gatcaaggtc
      781 gacgcaagcg gcctcttggc aggggacaag gatgccgaga acgccgcgaa gaaggcggtc
      841 cgagacgcgc tcaaggacta tgacggctgc cgggtcggca tcacgctgac cttcggctgg
      901 gatccggata tcaaccgcgg tctcgcaatc gccgatgcgg tcaacaagtt gctgccggac
      961 gccgagcccg ccatgttcga ggaagcggcc tacgaaaact tcggcaacct gcaaccggcc
     1021 ggtacagtcg agatccggat gtacttcttc cgcggctgcc aggcgctcaa caacgactga
     1081 gttgggcgcc tacccttcct cctccgtctc cggaggtgtg gtgcggtact ggtgcgcgag
     1141 cccggcataa cgatcggcgc cgagatggtg caggaggcgg cccgcctccg attcgacgtc
     1201 ccagaagcca tcgaacgatt cgtcatcggc cgcaactgcg accacctcgg cgatgaagag
     1261 gtcatgatca cccgccgtga ctcgatcccg aacgcggcat tcgatgtatc ccgggcaccc
     1321 ggtaatgcgt ggcggctcga tcacggtcga atccgcaggc tccaggtcga gcgctacgaa
     1381 cttgtcgccg tcccggccgg agatcatgcc ggcctcgtgg accgtgctca gccaatccag
     1441 caccgggacg ttgatgacga actcctcggt cgcggagacg aactggtgcg tcaggcggtc
     1501 cgggtggatc gcgaccgaga tcagcgtcgg gctgaggctc tgcgccgcaa cccaggcgac
     1561 cgtcatcacg ttcggctggc ccttgctcat cgaggagacc agcacgaccg gccccggcac
     1621 gatcaatcgc agggcatctc gcaatggtcg ctcgacaatc gtccccatct cagcgctgtc
     1681 cttcgttaga ccgtacatgc tcgtagaccg caacggtctg ctcagcggtc cggcgccagt
     1741 caaatgctcg ggagcgctcc aggccagcgc tgccaagacg gtgtgccaga gcatcgtcgg
     1801 tcaggattga ttggagcgcc gcagcgaccg gctcgacgcg tggctcgacg agcagaccgt
     1861 tgtccgcgac gatttccggg aagctcgtgc gattggcggc gattacggga attccgcagg
     1921 ccatcgcctc cagcacgctt agcccaaacc cctcgtagag cgacggcgtg acatagaccg
     1981 ccgcgccctg catcagcgcc actttgtcat cctcgctgat ccgtcccgtc agcacgaccc
     2041 ggtcgccaat atccagctcc gcaatcaacg gatccagcgg cgggaagacc ccgcggttgg
     2101 cgctgtgcgg cgccccgccg atgacgagct tcgtgtcgcc cg
//
LOCUS       DASSGX010000221         2531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1239208, whole genome shotgun sequence.
ACCESSION   DASSGX010000221 DASSGX010000000
VERSION     DASSGX010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1239208"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="VFI12_03865"
     CDS             complement(<1..812)
                     /locus_tag="VFI12_03865"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HET7055572.1"
                     /translation="MSERKSYDYIVVGAGAAGCVVANRLSADPGTTVLLLEAGGRDLN
                     PLIHIPIGFTKLTTPDVNWGYSTVPQPEMNNREMWYPQGRTLGGSTSINAMIYIRGHR
                     ADYDEWEALGNECWGYEGVLPFFRKSENNERFHNAYHGADGDLNVTQQVQHNPLTKAF
                     VRSCQEIGIDFNADVNGAGQDGVTYYDVTQRNARRESSATAFLKPAKKRHNLTVKTRA
                     MATRVLVENGRAIGLQVQTKDSLETIYANREVILSGGAVNSPKLLMLSGIGPA"
     gene            complement(906..2084)
                     /locus_tag="VFI12_03870"
     CDS             complement(906..2084)
                     /locus_tag="VFI12_03870"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HET7055573.1"
                     /translation="MNTEVRKTAVIEIERIVAGGYGLGRHDGRVLLVPLTAPGDTIEV
                     VLPERGAKATLNRIIVASPERVTTPCPYFGICGGCDLMHLSYPAQVEAKVQMVVETIE
                     RIGGQDLLNMSGGMGVEANPEPLHSRIRATWQPTPSHSAGYFRRGSHDVIEIDFCPIL
                     DPALESVRAGLRIHDKAQGLTNGSDVSLTTEGETADVIEFTVADAHILGSADVFFQAS
                     TSLLDRFVRHVVHLAGHGRPKHVLELYSGIGLFSVPLAPHVGTVDAVESAPLAVELAR
                     QNVQRNCLRNVECHVDSAEQWIATAGRVGTRPDAILVDPPRVGLSDRVAGGIIRLHPQ
                     RIVYVSCDPATFARDARLIADAGYRMTSLTMFDLFPQTHHTETVAAFERVESAPDQTA
                     "
     gene            2190..>2531
                     /gene="hisG"
                     /locus_tag="VFI12_03875"
     CDS             2190..>2531
                     /gene="hisG"
                     /locus_tag="VFI12_03875"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HET7055574.1"
                     /translation="MSKSMINGDNRLRLAIQRSGRLTDDTLNLLRLIGLEFESYGQRL
                     LSRCRNFPLSILYGRDDDIPGYVDMGTADLGIVGRNLVYEEGVDVEELVALGFGYCSL
                     VVAVMRDSPYQT"
BASE COUNT          429 a          813 c          791 g          498 t
ORIGIN      
        1 gccggtccga tgcccgagag catgagcagc ttcggcgagt tgaccgcgcc accgctcagg
       61 atcacctcgc ggttggcata gatcgtctcg aggctgtcct ttgtctggac ttgcaggccg
      121 atcgcgcggc cgttttcgac cagcaccctg gtcgccattg cccgggtctt gaccgtcagg
      181 ttgtggcgct tcttcgccgg tttgaggaaa gccgtcgccg agctctcgcg gcgggcgttg
      241 cgctgcgtaa cgtcgtagta ggtgacgccg tcctgtcccg ccccattgac gtcggcgttg
      301 aagtcgatcc cgatctcctg gcaagaacgg acgaacgctt tggtcaatgg gttgtgctgg
      361 acctgctggg tgacattcaa gtcgccgtcg gccccgtggt atgcgttgtg gaatcgctcg
      421 ttgttctcgg atttcctgaa aaagggcagc acgccctcgt agccccagca ctcgttgccg
      481 agcgcctccc actcgtcgta atccgcgcga tggccgcgga tgtagatcat ggcgttgatc
      541 gaggtgctgc cgccgagcgt gcggccctgc gggtaccaca tctcgcgatt gttcatctca
      601 ggttgcggga cggtcgagta gccccagttg acgtcgggcg tggtcaattt ggtaaagccg
      661 atcgggatat ggatcagcgg gttgaggtcg cggccgccag cctcgagcaa cagaacggta
      721 gtccctggat cagcgctcag tcgattggcc accacacagc cggccgcccc ggcgccaacc
      781 acgatgtagt cgtagctctt ccgctcactc atctgactgc gtcccttcgt tgagtgcgat
      841 gcgctggtcc gtgcgcgcac atcatgaagg aaccacacca gcgtcgcaac cgcggcgatg
      901 caggttcagg cagtctgatc cggcgccgat tcgacccgct caaacgcggc cactgtctcc
      961 gtgtggtgcg tttgcgggaa gaggtcaaac atcgtcaggc tcgtcatccg gtagccggcg
     1021 tcggcgatca ggcgcgcatc gcgggcgaag gtcgcagggt cgcacgagac gtagacgatc
     1081 cgctgcggat gcaggcggat gatcccgccg gcaacccggt cactcaggcc gacccggggc
     1141 ggatcgacca ggatcgcgtc cggtcgagtc ccgacgcgcc cggccgtcgc gatccactgt
     1201 tccgcgctgt cgacgtggca ttcgacgttt cgcaggcaat tccgctgcac gttctgccgg
     1261 gccagctcga ccgccaacgg cgctgactcg acagcatcca ccgttccgac atgcggagca
     1321 agcgggaccg agaacaggcc gatgccagaa tagagctcca ggacgtgctt tgggcgtccg
     1381 tgcccagcca ggtgcaccac gtggcgcacg aagcggtcga gcagcgatgt gctcgcctgg
     1441 aagaagacat cggccgagcc gaggatatgc gcatcggcga cggtgaactc gatgacgtcg
     1501 gcggtctctc cctctgtcgt cagtgagaca tccgatccgt tggtcagtcc ctgcgccttg
     1561 tcgtgaatac gcaggccggc tcgaactgac tccagtgccg gatcaaggat cgggcaaaag
     1621 tcgatctcaa tgacgtcgtg gctgcctcgc cggaaatagc ccgccgagtg cgatggcgtc
     1681 ggctgccacg tcgcccggat ccgggagtgc agcggctccg ggttcgcctc gacgcccatc
     1741 ccacccgaca tgttcagaag atcctggccg ccaatccgct cgatggtctc gacgaccatt
     1801 tgcaccttgg cctcgacctg tgccgggtac gagaggtgca tcagatcaca cccaccgcat
     1861 atcccgaaat atgggcacgg cgtggtgaca cgctcgggac ttgcgacgat gatccgattg
     1921 agcgttgcct ttgctccgcg ctcgggcagt acgacctcga tggtgtcgcc cggcgcggtc
     1981 agtggcacga gcagcaccct gccgtcatgc cgtccaagtc catatccgcc ggcgacgatc
     2041 cgctcgattt cgattaccgc tgttttgcgg acttccgtgt tcattcgccg tccgcttgtg
     2101 tgaaatctca caatttggga cactgatcgc ggctccgatg tctgcagccc cgttcccgag
     2161 accgcatccg acaaggaccg catccgccga tgtcgaaatc gatgatcaac ggcgacaacc
     2221 ggctccggct ggctatccag cgcagcggcc gtctgaccga tgacacacta aatctactcc
     2281 gcctgatcgg tctcgagttc gagagctacg gccagcgcct gctctcgcgc tgccggaact
     2341 ttcccctctc aatcctctat ggccgggatg atgacatccc gggttatgtc gatatgggca
     2401 ccgccgatct cggcatcgtc gggcgtaacc tcgtctatga ggaaggcgtc gacgtggagg
     2461 agctggtcgc gctcgggttc ggctattgct cgctcgtggt ggcagtgatg cgggattccc
     2521 cgtaccagac g
//
LOCUS       DASSGX010000222         3093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_476893, whole genome shotgun sequence.
ACCESSION   DASSGX010000222 DASSGX010000000
VERSION     DASSGX010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476893"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="VFI12_03880"
     CDS             <1..416
                     /locus_tag="VFI12_03880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HET7055575.1"
                     /translation="VAAGGTATDGTYTYSRVDSTFDCSLCPTADAGTAVQGGSGTTIN
                     LGSGFTYLLGKYDGPNAGDLIWNVTGLTSVTIPASWGPLTQQYGLSHWSVFNPTPSDG
                     PGDGPGEGPAPEPASLLLFGSALSAFGFRMRRALR"
     gene            737..1348
                     /locus_tag="VFI12_03885"
     CDS             737..1348
                     /locus_tag="VFI12_03885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HET7055576.1"
                     /translation="MKSALKKWMTIPAAAVACVGLASVAGATTLTYGDANFIGVVVSG
                     EPASLSNEAVYVNTLIDQAAGTSTAISGHTYDRTDSTFDCSGCPDAVGDSTAFQSLAS
                     PATTIDVSDGWTYLIAKYDGPQGGDLIWNIVGLTSVTIPGKWGPATTQYGLSHYALFN
                     PTEGPGDGPGDGPGEGPAPEPTSLLLFGSALSAFGFRMRRALR"
     gene            1395..3005
                     /locus_tag="VFI12_03890"
     CDS             1395..3005
                     /locus_tag="VFI12_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055577.1"
                     /translation="MPPPPFMFHLLQGIDARHASVLLKCAIAIWATMLLAWRASPANA
                     GGRGIPFADKTLAVLGLLAVLSWWHVGAYQFGVAFHRWEFFHYYLGAKYSPELGYTRL
                     YDCSAAAEAEDSPSPELLTRWTRNLETNVMERGSPAAIEPGLCRSRFTPARWLEFKHD
                     VLFFREEAKTDWLRMQQDHGYNATPVWNAAGYLLANTGPATKRQMVLIALIDPALLAL
                     MWVAVWRTFGWRPAAVAAIWWGTNFPARYFYLGGSFVRSDFLVAAVLAVVLAKRGYMM
                     MAGAATCWSTLLRIFPGFLISGPVVRALTGEPGARSSLKRSLGPLVRFAAGGCIAVCV
                     LIPFSLLSPPGGVSAGIGRWTGFVRNSDKHLSGSSVNRVGLKPLLSFTRGGDYRSLRD
                     FWIENPDDAWQAVRHQTFQGRRWLFWGLTAAFLVLLGLAARGQPYWVALVLGVGLIPI
                     VSDIACYYYGILLVYGLLWDRYPWTGVGLASLSVFTLIAGELSSTEEVRYLIISAGIV
                     FYVFAVTGGIARSNMAAWRQRVIEQPAA"
BASE COUNT          485 a          967 c         1022 g          619 t
ORIGIN      
        1 ttgtggccgc tggtggcacc gcgacggacg ggacgtacac gtacagtcgg gtagattcaa
       61 ccttcgattg cagcttgtgt ccgacagccg acgccggcac ggccgtccag ggcggctccg
      121 gcacgacgat caacctcggt agcgggttca cgtatctgct tggcaagtac gacggcccga
      181 acgctggcga tttgatctgg aatgtgacgg gattgacgag cgtcacgatt ccggcctcgt
      241 ggggccctct cacgcaacag tacggtctct cccactggtc ggtcttcaac ccgacaccat
      301 ccgatggacc tggagacggc ccgggtgagg gtccggctcc ggagccggca tcgctcctgt
      361 tgttcggctc ggcgctgagc gcattcggct tccgtatgcg ccgggcgctt cgttaactcg
      421 cagtaatcgg tttccacgcg acgaagggac ggcacgatgt gccgtccctt ttttatgtcc
      481 cggctgtctg ccggaccaat ccaagccgca cgactccctc cagggcaaat taagtcgacc
      541 ggacaggtga cttccgccaa ctgtcagtac cgtctaagct atttttattc aacgagttac
      601 gagaatggcg tacacggccg tatgtttgca gagtgagtcg atgctctgcg gcaaccgagg
      661 gcttttctgt cgtcagggcg acgtaggcga tgtcttgact acgccgcgta caaacactct
      721 caacaggaag gaccctatga agtcggcact gaagaaatgg atgacgatcc cggcggcggc
      781 cgttgcctgc gtgggactcg catccgtcgc tggcgcgacc acactgacgt acggtgatgc
      841 gaatttcatc ggcgtcgtgg tgtctggcga gcccgcgagc ctctcgaatg aagcggtgta
      901 tgtcaacacc ctgatcgatc aggcggctgg caccagcacc gcgatcagcg gccacacgta
      961 cgatcggacg gactcgacgt tcgactgtag cggctgtccg gacgccgttg gcgacagcac
     1021 cgcgtttcag tcgctggcga gccccgcgac cacgatcgat gtcagcgacg gctggacgta
     1081 cctgatcgcc aagtacgacg ggccgcaggg cggcgatctg atttggaaca tcgtcgggtt
     1141 gacgtcggtc acgattcccg gcaagtgggg cccggcgacc actcagtacg gtctgtcgca
     1201 ctacgccttg ttcaacccga ccgagggccc cggtgacggt cctggcgacg gtccgggcga
     1261 agggccggct cccgagccga cgtcgctcct gctgtttggc tcggcgctga gcgcattcgg
     1321 attccgtatg cgccgggcgc tccgttaagc gcagcactgc tcggttctcg aatacaccag
     1381 acgggcggta caatgtgccg ccccctcctt tcatgttcca cctcctgcag ggcatcgacg
     1441 cgcggcacgc gagcgtgctt ctgaaatgcg cgattgccat ctgggcgacg atgttgctcg
     1501 cctggcgggc atcccccgcc aacgcaggtg gccgagggat accctttgcc gacaagaccc
     1561 tggcggtcct cggcctcctc gcggtcctga gctggtggca cgtcggcgcg tatcagttcg
     1621 gcgtcgcctt ccatcgctgg gagttctttc actattacct gggcgcgaag tacagccccg
     1681 aactggggta tacgaggctg tacgactgct cggctgcagc ggaggcggaa gacagcccct
     1741 ctcctgagct cctgacgcgc tggacccgga acctggaaac gaacgtcatg gagcggggat
     1801 cccccgcggc catcgagcca ggcctctgcc ggtcgcggtt cacgccagcg cgctggctgg
     1861 agttcaagca cgacgtgctg ttcttccggg aagaggcgaa gaccgattgg ctgcgcatgc
     1921 agcaggacca cgggtacaac gcgactccgg tctggaacgc ggccgggtac ctgctggcga
     1981 acacggggcc ggcgacgaag cgccagatgg tcctgatagc cttgatcgat cccgcactgc
     2041 tcgccctgat gtgggtggcg gtgtggcgga cgtttggctg gcggccagcg gcggttgccg
     2101 ccatctggtg gggcacgaac tttccggcga ggtacttcta tctcggggga tccttcgtgc
     2161 ggagcgactt tctggtcgcg gccgtgctcg cggtcgtgct ggcaaagcgc gggtacatga
     2221 tgatggcggg tgcggccacc tgctggtcga cgctcctgcg catctttcct ggcttcctga
     2281 tcagcggacc ggtggtgcgc gcactgacgg gagagccggg agcccgctcg tccctgaagc
     2341 gctcgctcgg gcccctcgtg cgtttcgcgg ccggtggctg catcgccgtc tgcgtgctga
     2401 tccccttttc cctgctcagc cctcccggcg gcgtctccgc cggcatcggc cgctggacag
     2461 gtttcgtccg caacagcgac aagcatctga gcggaagctc ggtcaaccgc gtcgggctca
     2521 agccgctgct gtcgttcacg cgcggcgggg actatcgaag cctccgcgat ttctggatcg
     2581 agaatccgga cgatgcgtgg caggcggttc gccatcagac cttccagggc cgtcgatggc
     2641 tgttctgggg gctcaccgcc gccttcctgg tattgcttgg acttgccgcg cgcggccaac
     2701 cctattgggt cgcgctcgtg ctcggagtgg ggctgatccc gatcgtctcc gacatcgcgt
     2761 gttactacta cgggattctg ctcgtgtacg ggttgttgtg ggaccgatat ccgtggacgg
     2821 gtgtcggtct ggcgtcgctg tcggtgttca cgctgatcgc cggtgagttg tcttctaccg
     2881 aagaagtccg gtatctgatc atcagcgcgg gcatcgtgtt ctatgtgttc gcggtcacgg
     2941 gcggtatcgc tcggagcaat atggccgcct ggcggcaacg ggtgatcgag cagcccgcgg
     3001 cctgacgctc gcgctgatcg cggcaggttc gcgcgatacc agtcgattgg aacggtcggg
     3061 ccgtgggaag tgtatgcgca gcgtcagcgc ctg
//
LOCUS       DASSGX010000223         2675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_914787, whole genome shotgun sequence.
ACCESSION   DASSGX010000223 DASSGX010000000
VERSION     DASSGX010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914787"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /locus_tag="VFI12_03895"
     CDS             complement(<1..824)
                     /locus_tag="VFI12_03895"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7055578.1"
                     /translation="MSVREKNDSVSRLIHDSIDQRVSRRDILRRGAMLGVAAPMLAAM
                     IKVSETTAVFAQDAGEIVVGCPYNLTGSLQSIDVPAKDGSLLAAKLLNSAGGVLGKQL
                     KLVVENGESDVTVVSNICKKMIDEDKVVALVGLTDTDYVRAGGQVAQEKGIPFIDVGG
                     TAPIIAQIGDFMFLLPFGDNVQAAVEAEFAQEKGWKTAAMLVNDGMTYTKFLAKYFKD
                     RFTMDDIGGQVIKELTYKVGDTDYSSQVTEIKGLNPAPDVLMISAGPEEIGTIVKQV"
     gene            complement(929..1984)
                     /locus_tag="VFI12_03900"
     CDS             complement(929..1984)
                     /locus_tag="VFI12_03900"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET7055579.1"
                     /translation="MSKLATYGVAILILAGFLWFAQDTFTSYRQQLVVLVAINIVVAV
                     SLTMQSGFTGVFSLGQVGFMAIGAYVGALINLPVSWKDPIILPGLPGWLATFDTSGWP
                     DWLALAFACVVGGLAAMAVAVVVGYPLMRLSGNYVAVATMGFLIIVYTVLIQWDSVTY
                     GSQGLNPIPSYTNVWSAYFWAVLTIYVAWRIRRSSFGRSMIASRDNLIAARAVGVRVH
                     RARLLAFTVCGFLTGVAGCLLGHQLSIVSPSAFYFTTTFNIIIMVVLGGLGSITGAIF
                     GALFMTLAPELLRNLETNVTIGPIHWDQAFGLQQIILALIFIAVMILRPRGLMAGREI
                     DLAWWQNRRQAQPEGPE"
     gene            complement(1985..>2675)
                     /locus_tag="VFI12_03905"
     CDS             complement(1985..>2675)
                     /locus_tag="VFI12_03905"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET7055580.1"
                     /translation="VIGTIIAGLLMERVAYRPLRGAPEISLLLTSLGVSIAVRNSASM
                     IFGVQPKKFPTPGSFEKRLGIGGEVRISVTNLITIVVALILLALLTWFVTRTRLGIQM
                     RATAENPMAARLMGINTTTVIVTAFIIGSALAGVAGVLWGARVGRIEPLMGFVPVLKA
                     FVATVIGGFGSIPGAVIGAFILGFGEIFLVALLPDHLTGYRDAFIFAALIVILLVRPQ
                     GILGSTEPEKI"
BASE COUNT          535 a          884 c          819 g          437 t
ORIGIN      
        1 acctgcttga cgatcgtgcc gatttcctcc ggaccggccg agatcatcag cacgtccggc
       61 gccgggttga gacccttgat ctcggtgacc tggctggagt aatcggtgtc gccgaccttg
      121 taggtcaact ccttgatcac ctggccaccg atgtcgtcca tcgtgaagcg gtccttgaag
      181 tacttggcca ggaatttggt ataggtcatg ccgtcgttga cgagcatggc cgccgtcttc
      241 cagcccttct cctgcgcgaa ttcggcctcg acggccgcct ggacgttatc gccgaacggc
      301 aagaggaaca tgaaatcgcc gatctgggcg atgatcggcg ccgtgcctcc gacgtcgatg
      361 aacgggatgc ccttctcctg cgccacctgg ccgccggccc gaacgtagtc ggtgtcggtc
      421 aggccgacga gggcaacgac cttgtcctcg tcgatcatct tcttgcagat gttcgagacg
      481 acggtcacgt cgctctcgcc gttctcgacg acgagcttca gctgcttgcc gaggacgccg
      541 ccggcggaat tcagcaactt ggcggccagc agcgagccgt ccttggccgg gacgtcgatc
      601 gactgcaacg agccggtcag gttatatggg cagccaacga cgatctcgcc cgcgtcctgc
      661 gcgaagaccg cggtcgtctc gctgaccttg atcatcgccg ccagcatcgg cgcggccaca
      721 ccgagcatcg ctccacgccg cagaatgtca cggcgggata cgcgctggtc gatcgagtcg
      781 tgaatcaggc gggaaacgga gtcgttcttt tcacggacgc tcacgcgttg ctcctctcgc
      841 cgggtatgtc cggctcagac tcaagcgacg tccttccgct gtctaccgta gacagccgcg
      901 tcgcacgtac atccagtgcc ttcggattct actccggacc ctccggctgc gcttggcgcc
      961 gattctgcca ccaggccagg tcgatctccc ggccggccat caatcccctc ggccgcagga
     1021 tcatcaccgc gatgaagatc agcgccagga tgatctgctg caggccgaat gcctgatccc
     1081 agtgaatcgg gccgatcgtg acattcgtct cgaggttacg caaaagctcc ggcgcgagcg
     1141 tcatgaagag cgcgccaaag atcgcgccgg tgatgcttcc cagaccgccg agcacgacca
     1201 tgatgatgat gttgaaggtc gtggtgaagt agaaggcgct gggcgaaacg atgctcagct
     1261 gatgtccgag gaggcagccg gcgaccccgg tcaggaagcc gcaaacggtg aaggccagca
     1321 gtcgcgcgcg gtgcacccgc acgccgactg cccgggccgc gatcaggttg tcgcgcgagg
     1381 cgatcatcga gcgcccgaag ctcgacctcc ggatgcgcca ggcaacgtag atagtcaaca
     1441 ccgcccagaa gtaggccgac caaacattcg tgtacgaggg aatcgggttc aggccctgcg
     1501 atccataagt cacgctgtcc cactggatca gcaccgtgta gacgatgatc aggaagccca
     1561 tcgtcgcgac ggccacatag ttgccggaga gccgcatgag cggatacccg acgacgaccg
     1621 cgaccgccat cgccgccagc ccaccgacca cgcaggcaaa ggcgagcgcc agccagtccg
     1681 gccagccgct cgtgtcgaag gtcgccagcc agcccgggag gccgggcaag atgatcggat
     1741 ccttccagga aaccggcaga ttgatcaacg ccccgacata cgcgccgatc gccatgaagc
     1801 cgacctgccc gagcgaaaag acgccggtga atccgctctg catcgtcagg ctgaccgcga
     1861 cgacgatgtt gatcgcgacc agcacgacca gctgctgacg gtagctcgtg aaggtgtcct
     1921 gcgcgaacca caggaagccc gccaggatca ggattgcgac accgtaggta gcaagcttgc
     1981 tcatctagat cttctccggc tcagttgatc caaggatgcc ctgcggccgg accaggagaa
     2041 tcacgatcaa tgccgcgaag ataaaggcat cgcggtagcc ggtcaggtga tcgggcagga
     2101 gcgcgaccaa aaagatctcg ccgaatccca gaatgaacgc gccgatcacc gcgccgggga
     2161 tcgagccgaa cccgccgatc acggtcgcca cgaacgcctt gaggaccggg acgaagccca
     2221 tcagcggctc gatccgaccg actcgcgcgc cccagaggac accggccacg cccgccaacg
     2281 ccgaaccgat gatgaaggcg gtcacgatca cggtcgtcgt gttgatgccc atcagccgcg
     2341 cggccatcgg attctccgcc gtcgcccgca tctggatgcc gaggcgggtt cgtgtcacga
     2401 accaggtcaa caacgcaagc aggatcaggg cgacgacgat cgtgatcaag ttcgtcacgc
     2461 tgatccgcac ctcgccgccg attccgagcc gcttctcgaa gctgccgggc gtcgggaact
     2521 tcttcggttg caccccgaag atcatcgaag cggaattgcg aacggcgatg gacacgccga
     2581 gcgatgtcag caggagcgag atctccggcg cgccccgcag cgggcggtac gccacccgct
     2641 ccatcaggag gccggcgatg atcgttccga tcacg
//
LOCUS       DASSGX010000224         3299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1239825, whole genome shotgun sequence.
ACCESSION   DASSGX010000224 DASSGX010000000
VERSION     DASSGX010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1239825"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VFI12_03910"
     CDS             <1..285
                     /locus_tag="VFI12_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055581.1"
                     /translation="GLGMSRGDLRDSPFSTFAVPGLILSIAVGGSQIAAAVALLKRHR
                     HAALLALAAGLVMLGWIVVQSVMIPSGRGLQAFILAYAILEIRMAWSRGR"
     gene            complement(271..2892)
                     /locus_tag="VFI12_03915"
     CDS             complement(271..2892)
                     /locus_tag="VFI12_03915"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HET7055582.1"
                     /translation="MSTQQTTSADPSPMLDAARVAGEELTGLTSAEVEERRAQGLSNT
                     VSITSTRPYPEIIRHNVFTLINVVLYATCAMLIAIGLYGDAVVTIGLVLFNVVISILQ
                     EVRTKWSLDKISVLTWPRATVIREGEERIVDPSELVQADLLVVAAGDQIVADGPLVGG
                     KGIEVDESLLTGESEPVHKAIGDKVLSGTFCVSGKGIYRAETVGLSSVANRLTRGARE
                     FRQSKTPMQQDMDLIVRGTVLAVVFVGGPVLLDLFARAIAVVIRRIDQPSSTAIRLAF
                     GGYPVEDTVRSAAVILALIPQGLALMVAVTYAAAALRLAGKGVLIQQTNAMESLSHVD
                     VLCLDKTGTLTTNELFVDEVAPFGVERGELEQCAGDFAANTTSTNKTSEAIGNAFAGR
                     RRRVVADVPFNSARKWSAMTVDDDHGQGVFVMGAPDVLAPHLSDREGIGDVVADWSGK
                     GRRVILLACNPDPRSLDDANDAPQLPELQPIGLIGLVDGLRDESRETLKHFQDLGVRL
                     KLISGDDPRTVAALARNAGFPIEGDVISGSDLGQMDDDAFAECAARGSVFGRTTPDQK
                     ERLIGALQRDGKYVAMTGDGVNDVPALKRANMGIAMRSGSDAARGVADLVLLEDSFGA
                     LPKAFAEGQRIVSGMQDIAALFLVRSLSVMLILVGAAFVTVPFPLTPRDNALLAFLTV
                     GIPTLGLAAWAKPETARNRLLRPIARFAIPASIMVATLSLITFMTWWRLTHDIDESQS
                     VLTTTAVLCGLILLPFIQPPVQWFVGGDEYSGDWRPTLLAAGLFGGFLGIAYTPALQR
                     LFGFARVDPRNLAILVVIVGVWAVALRWVWRSQFFQRIAGLRSSGQPAGSAVATALTE
                     TDVASAAGQSGQQSPSA"
     gene            3050..>3299
                     /locus_tag="VFI12_03920"
     CDS             3050..>3299
                     /locus_tag="VFI12_03920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055583.1"
                     /translation="MRTSGGNLVLAVNSGSSSLKIGLFEPGESLREIGSIEADGVGTD
                     AARIRSAGVLNSPSPSGLRLPDHATAIQAVLDHLDATGI"
BASE COUNT          600 a         1080 c         1044 g          575 t
ORIGIN      
        1 ggtctcggga tgagtcgggg cgatctcagg gactccccat tcagcacctt cgccgtgccg
       61 gggctgattc tgtcgatcgc ggtcggcggc agccagatcg cggcggccgt cgccttgttg
      121 aaacgccacc gccatgcggc acttctcgcg ctggcggcag ggctcgtgat gctcggctgg
      181 atcgtcgtcc agtcggtaat gatcccgagc gggcgggggc tgcaggcgtt catcctcgcc
      241 tacgcgatcc tcgagatcag gatggcctgg tcacgcggac ggtgattgct gcccggactg
      301 gccggcggct gaagctacgt cggtctcggt cagtgccgta gcgaccgcgc tgcccgccgg
      361 ttggcccgat gatcgcaacc ccgcgatgcg ttggaagaat tgcgatcgcc agacccaacg
      421 caacgctacc gcccagacgc cgacgataac gaccaggatc gccaggttcc gggggtcgac
      481 tcgggcaaat ccgaacaacc gctgcagggc cggcgtgtat gcgatgccga gaaatcctcc
      541 gaacagtcct gccgccaaga gcgtcgggcg ccaatcgccg ctgtactcgt cgccgccaac
      601 gaaccattgc accggcggct ggatgaacgg caacaggatc aggccgcaga gcacggcggt
      661 agtcgtcaac accgattgcg actcgtcgat gtcatgggtc agccgccacc aggtcatgaa
      721 cgtgatcaga ctcaacgtcg cgaccatgat cgaggccggt atcgcaaacc gggcaatcgg
      781 tcgcaataac cggtttcggg ccgtttccgg cttggcccac gccgcgagac cgagtgtcgg
      841 gatgccgacc gtcagaaagg ccagcagtgc gttgtctcgg ggcgtgagcg gaaacggaac
      901 cgtgacgaac gccgccccga ccaggatcag cattactgag agcgagcgga ccaggaacag
      961 cgcggcgata tcctgcatgc ccgagacgat ccgctgccct tcggcgaacg ccttcggcaa
     1021 cgcgccaaat gagtcctcga gcagaacaag gtcggccaca ccgcgggcgg catcacttcc
     1081 gctccgcatc gcgatgccca tgttcgcccg cttgagcgcc gggacgtcgt tgacgccgtc
     1141 gcccgtcatc gccacgtact tgccatcccg ctgcagcgcc ccgatcagcc gctccttttg
     1201 gtccggcgtg gtgcgcccaa acacggagcc gcgggctgcg cactcggcga acgcgtcgtc
     1261 gtccatctgc cccagatcac tcccggagat gacatccccc tcgatcggga atccggcatt
     1321 gcgggccagc gcggcgactg tccttggatc gtcgcccgaa atcagcttca ggcggacgcc
     1381 caggtcctgg aaatgcttga gcgtctctcg ggactcatcg cgaaggccat ccacaagccc
     1441 gatcaatccg atgggctgca actcgggtaa ttgcggcgcg tcattggcgt cgtccagcga
     1501 gcgcggatcg gggttgcagg ccaacaagat cacccgtcgc cccttcccgg accaatccgc
     1561 gacgacatcc ccgatgcctt cgcggtcgct gagatggggc gcaaggacgt ccggagcacc
     1621 catgacgaac acgccctgac cgtggtcgtc gtcgaccgtc attgcgctcc acttgcgcgc
     1681 ggagttgaac ggcacgtccg cgacgactcg tcgccggcgg ccggcaaatg cattgccgat
     1741 tgcctcggat gtcttgttgg ttgacgtggt attcgcggcg aagtcccccg cacattgctc
     1801 cagctcgccg cgctcgacgc cgaatggagc cacttcgtcg acaaagagct cgttcgtcgt
     1861 cagcgtccct gtcttgtcca ggcagagcac atcgacatgg ctgagagact ccatggcgtt
     1921 cgtctgctga atcagcactc ccttgccggc cagccggagc gccgccgccg cgtaggtcac
     1981 cgcgaccatc agggccagac cctgtgggat cagcgcgagg atgaccgcgg ccgagcggac
     2041 cgtgtcttcg accgggtaac caccaaaagc cagccggatc gcggtcgagc tgggttgatc
     2101 aatccggcgg atgacaactg cgatcgcccg cgcgaacagg tccagcagca ctggcccgcc
     2161 aacgaaaacc accgccagga cggtgccgcg cacgatcaaa tccatatcct gctgcatcgg
     2221 tgtcttcgac tgccggaact cgcgcgcgcc gcgcgtcagc cggttcgcca cgctcgacag
     2281 accgaccgtc tccgcccggt agatcccctt gccagagacg cagaaggtgc cagagaggac
     2341 cttatcgccg attgccttgt ggactggctc ggactcgccg gtcaggaggg actcatcgac
     2401 ctcaatacct ttcccaccga cgagcggacc gtccgcgacg atctggtcac ccgccgcgac
     2461 gaccagcaga tccgcctgca ccagttcgga cgggtcgacg atacgctcct cgccctcgcg
     2521 aatcaccgtc gcgcgtggcc aggtcagcac ggagatcttg tcgaggctcc acttggtgcg
     2581 aacttcctgg aggatggaga taaccacgtt aaagaggaca aggccgatcg taaccaccgc
     2641 atcgccgtaa aggccgatcg cgatcaacat ggcgcaggtg gcgtagagga cgacgttgat
     2701 cagggtgaag acgttgtgcc ggatgatttc gggatacgga cgagtactgg tgatggagac
     2761 ggtattgctc agcccttgtg cgcgacgctc ttcgacttcg gcgctggtca ggccggtcag
     2821 ctcctcgcca gcaacccggg ctgcgtcgag cattggcgat ggatctgcgg aggttgtctg
     2881 ctgcgtgctc atccggaatc cgcttcaatt cgcacgtcgg tgtagtaacc gcgctccccg
     2941 ttttcggcat tgtcgacgat acgttaccgg ggcgtcgtga ttgcgcgaga cagatgactc
     3001 gctggttccg cgccgggtta gttccaggaa agaatggtgg acacggctcg tgaggacgtc
     3061 cggcggcaac ctggtacttg cggtgaattc ggggtcgtcg agcctgaaga tcggactctt
     3121 cgagccgggg gagtcgctgc gggagatcgg ttcgatcgag gcagatggtg tcgggaccga
     3181 cgctgcgcgg attcgatctg ccggagttct caattcaccc tcaccgtccg gtttgaggtt
     3241 gcccgaccac gcgacggcga ttcaggcagt gctcgaccac ctcgatgcga ccggcatca
//
LOCUS       DASSGX010000225         4941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1568362, whole genome shotgun sequence.
ACCESSION   DASSGX010000225 DASSGX010000000
VERSION     DASSGX010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4941
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1568362"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VFI12_03925"
     CDS             <1..272
                     /locus_tag="VFI12_03925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET7055584.1"
                     /translation="SENGLGMPRYAYECQHCGAEFDQERPIADAAQPAACPVCGESAD
                     RVITPPRFLFKADPRDNRPYWHNHDGYSHSHPPRRGRHRTPEEDH"
     gene            complement(615..1550)
                     /locus_tag="VFI12_03930"
     CDS             complement(615..1550)
                     /locus_tag="VFI12_03930"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET7055585.1"
                     /translation="MTDHPFTSVLVPLDGSQLSEQALPFALAIVKEGGSVTLLQAIPD
                     AEPLRKPFGAVTMSADEVRQMLTNLADADLDRAAAHWSQLAPNVSISKETTQGDAATV
                     ILDAADSKNADLIVLASTGRGALGRLTLGSVADRVVRTAERPVLVVRGFDAPTAQALP
                     QVMRVVVPLDGSDRSMLAIPVAAAMATQLNAAVELVMAVDLPQIVSPAMAYGPAFSPE
                     FYAEVEEATTRNANEFLDGAVAEFGKLGVTATKHVMLGAASAAIIDFAKQGDLVVMTS
                     RGQGGIKRWILGSVAEKLIRDSPAPVLLVPSHHHE"
     gene            complement(1651..1842)
                     /locus_tag="VFI12_03935"
     CDS             complement(1651..1842)
                     /locus_tag="VFI12_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055586.1"
                     /translation="MDGNSDWHTSNGPLIGLIAAVVGLNAVVLSSDLNDWIRYAVGGV
                     GVIVLVLCVYKLGKNQWRS"
     gene            complement(1867..2667)
                     /locus_tag="VFI12_03940"
     CDS             complement(1867..2667)
                     /locus_tag="VFI12_03940"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7055587.1"
                     /translation="MSKKKVLITGVAGVVGQACVQYLSDRYELSGLDIKDPGNVPTLV
                     ADIADLNAILPAFEGVDAVVHLAGNPSVHSPWDDVFTINIQGTYNAYEACRIQGVHCL
                     VFASSNHTIGRYEEEGMPQIYALDDPRCYDETVPVRPDSLYGVSKLFGEAVGRYYNEY
                     FGIRSYNLRIGTVRADDDPRDPSVAEGSFWLDLTPEQKFDRLRATWLSKRDCCDLIAT
                     CIEAANVPFAIVYAISNNPRQFWDLSHAKELLGWEPKDSAPIGPAEKR"
     gene            2765..3679
                     /locus_tag="VFI12_03945"
     CDS             2765..3679
                     /locus_tag="VFI12_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055588.1"
                     /translation="MNNLPFDKPGQFWKTNLHSHTTRSDGKLTPSELATLYRDRGYHA
                     LAITDHFLDRFGFPIVDTTGFRTENFTTLLGAELHGPGLMLGDWHILAVGLPPDFAPA
                     TNGETGPEIARRASDAGAFVALAHPAWYSLFFEKAKEIDFADAIEVFNTTCLYDNDRG
                     ESWYLADQFLMTGRRPFAIATDDAHCSLRPDTFGGWTMIKSASLDPESLLAALKAGAF
                     YASTGPDIHNVAVEGDKLIVECSPARTIYATGAGALHKDVGGTGITRAEFDIEPFTQS
                     YARITILAEDGKAWSNPIFFDQGPAPKS"
     gene            complement(3969..>4941)
                     /locus_tag="VFI12_03950"
     CDS             complement(3969..>4941)
                     /locus_tag="VFI12_03950"
                     /inference="COORDINATES: protein motif:HMM:NF014212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055589.1"
                     /translation="ESFEPLNNEEIDLSQPHVMTVLGPIDPGALGFTLHHEHVFNLTN
                     PLAAGDDDLVLDDIANSRLDLDVYFAIGGRSIVDMGPADYGRSVEAMLQIAQHAPVNI
                     VMVTGHHKHLIAVPCVGNDSVEAITERNLRELREGIDGTDVRAGLIKAGTSLNEITDV
                     ERRVLEAAARTQVAFGAPISTHTEQGTMALEQIEIMAAAGADPGRITLCHMDHRLHET
                     EYLIEVLKTGAFISFDRWTRVQLASDDERAEVLLRLADAGYLDQLLVSGDFARKSNHL
                     GYGAAVGFDYFIDRVPLMLMGAGFDAPSVRRIFVENPARMLTIYPVT"
BASE COUNT          876 a         1815 c         1426 g          824 t
ORIGIN      
        1 cgagcgagaa cggattaggg atgccccggt acgcatacga atgtcagcac tgcggcgcgg
       61 aattcgatca ggagcgccca atcgccgatg cagctcagcc ggccgcatgc cccgtctgcg
      121 gcgagtcagc agaccgcgtc attaccccgc cccgcttcct cttcaaagcc gacccgcgcg
      181 acaaccgccc ctactggcac aatcacgacg gctactccca cagccacccg ccccgccgag
      241 gccgccaccg caccccggaa gaagaccact gatggagacg agcaggcgaa gacgactgta
      301 gggatttgcg ggggcaaatc cgccccttcc caagcgccgg ttgccgatga cgagaatgcc
      361 tgcgggggca gacggcatct gtccgctcgt tgggtaaagc cggtacttca ataggggcgt
      421 agcgcggacc ccccgtgggc cgcatcatgg atccccgggg acgttcgatg gacccgatcc
      481 ggtatcaggt ccgggccggc cacaggggtc agccaatacg cctcagcgtg ccggccggcc
      541 tcggccacca catttccttc agccagggcg gcatcgggac gcccggttgg acccgtagag
      601 cctgtggccc agccctattc gtgatgatgc gacggcacca gcagcaccgg cgccgggctg
      661 tcccggatca gcttctcagc cacgctgcct aaaatccaac gcttgatccc gccctgcccc
      721 cggctcgtca tcacgaccag gtcaccctgt ttcgcgaaat cgatgatcgc cgcgctcgcc
      781 gccccaagca tcacgtgctt ggtcgccgtc accccgagct tcccgaactc ggccaccgca
      841 ccgtccagga actcgttcgc attccgcgtc gtcgcctctt cgacctccgc gtagaactcg
      901 ggcgagaagg ccggcccata cgccatcgcg ggcgatacga tctgcggcaa atcgaccgcc
      961 atcaccaact cgaccgccgc gttcaactgc gtcgccatcg ccgccgcaac cgggatcgcc
     1021 agcatcgagc ggtccgagcc atccagcggc acgacgaccc gcatcacctg cggcagcgcc
     1081 tgtgccgtcg gcgcgtcgaa cccgcgcacg accagcaccg gccgctccgc cgtccgtacc
     1141 acccggtcgg ccacgctgcc gagcgtcaac cgtcccagcg ccccgcgacc ggtgctggcc
     1201 agcacgatca gatcggcatt cttcgaatct gccgcatcga ggatcaccgt cgccgcgtcg
     1261 ccttgcgtcg tctccttgct gatcgagacg ttcggcgcga gctgcgacca gtgcgccgcc
     1321 gcccgatcca ggtccgcatc cgccagattc gtcagcatct gccggacctc gtcggccgac
     1381 atcgtgaccg cgccgaatgg cttccgcagc ggttccgcgt ccgggatcgc ttgcaggagc
     1441 gtcaccgacc cgccctcttt gacgatcgcc agcgcgaagg gcagtgcctg ctcgctgagc
     1501 tgggagccat ccagcgggac aagcacggac gtgaacgggt ggtcagtcat cgcaaaactc
     1561 cttgcctgcc gggataattg ggccatctgc ctgcagtgta ccgccttgcc cgaacaggta
     1621 ggtttgagtc accgccgggg aatagtggca tcaactccgc cactggttct tgcccagctt
     1681 gtaaacgcag agcaccagga cgatcacacc cactccgccc acggcgtagc gaatccagtc
     1741 attgaggtcg ctgctgagta cgaccgcgtt gagtcccact accgccgcga tgagaccaat
     1801 cagcggaccg ttcgaggtgt gccaatccga attgccatcc aacgttctct ccttgccttg
     1861 aagcgatcac cttttttcgg cagggccgat cggcgcgctg tccttcggct cccagccgag
     1921 caactccttc gcgtggctga gatcccagaa ctgccgcggg ttgttggaga tcgcgtagac
     1981 gatcgcgaac ggtacgttcg cggcctcgat acaggtcgcg atcaggtcgc agcagtcccg
     2041 cttcgagagc caggttgccc gcagccggtc gaatttctgc tcgggcgtca gatccagcca
     2101 gaacgagccc tctgccacgc tcggatcgcg cgggtcgtca tccgcccgga ccgtgccgat
     2161 ccgcaggttg taggagcgaa tcccgaagta ctcgttgtaa taccggccga ccgcctcgcc
     2221 gaacagcttg cttacgccgt acagcgaatc cggccggact ggaacggttt cgtcgtaaca
     2281 acgcggatca tcaagcgcgt agatctgcgg catcccctcc tcctcgtatc gcccgatcgt
     2341 gtggttggac gaggcgaaga cgaggcagtg cacgccctgg atccggcacg cctcataggc
     2401 gttgtaggtc ccctgaatgt tgatggtgaa gacgtcgtcc cacggcgaat ggaccgacgg
     2461 gttcccggcc agatggacga ccgcatccac cccctcgaac gccggcagga tcgcgttcag
     2521 gtcggcgatg tccgcgacca gcgtcggcac attgccggga tccttgatgt cgagcccgct
     2581 cagctcgtat cggtccgaca ggtattgcac gcaggcctgg ccaaccaccc cggccacgcc
     2641 cgttatcaga acctttttct tgctcaatgg aagctcccct gctcgctccg gcaaagacgg
     2701 cacgccgggt atcctaccaa ccagcgattt ccggcgatga ttgacgtgaa caggatcaag
     2761 cgcggtgaac aacctcccgt tcgacaagcc agggcagttc tggaagacca acctccactc
     2821 gcacaccacc cgctccgacg gcaagctgac gccgtctgag ctggccaccc tctaccggga
     2881 tcgcggctac cacgccctcg ccatcaccga ccacttcctc gatcgcttcg gcttcccgat
     2941 cgtcgacacc accggcttcc ggactgagaa cttcacgacg ctgctcggcg cagagctgca
     3001 cgggcccggc ctgatgctcg gcgactggca catcctcgcc gtcggccttc cgcccgattt
     3061 tgccccggcc acgaacggcg agaccggccc cgagatcgcc aggcgggcca gcgacgccgg
     3121 cgcattcgtc gcgcttgccc accccgcctg gtactcgctc ttcttcgaga aggcgaagga
     3181 gatcgatttc gccgacgcga tcgaggtctt caacacgacc tgcctctacg acaacgaccg
     3241 cggcgagagc tggtacctgg ccgaccagtt cctgatgacc ggccgccgcc ccttcgccat
     3301 cgccaccgac gacgcccatt gcagcctccg cccggacacc ttcggcggct ggaccatgat
     3361 caagagcgca tcgctcgatc ctgagtcgct cctcgccgcc ctcaaagccg gcgcgttcta
     3421 tgcgtcgacc ggccccgaca tccacaacgt cgccgtcgag ggcgacaagt tgatcgtcga
     3481 gtgctccccg gcccgcacca tctacgcgac cggcgccgga gccctccaca aggacgtcgg
     3541 cgggaccggc atcacgcgcg ccgagttcga catcgagccc ttcacccaga gctacgcccg
     3601 catcaccatc ctcgccgaag acggcaaagc ctggtccaac cccatcttct tcgaccaagg
     3661 cccggcaccc aaatcgtaat cgcggtgctc cgtacccgcc ttgagcgccg ctccaaatcc
     3721 cggccgcatg cgcgggcccg tttttccacc gtaggggcgg cgcttgctcc gcccttcatc
     3781 catccccggg tcccatcgat ggggggaggg cgctcggccg gctccaggca tcgggcgggt
     3841 ccgcaacggg ggagcggcaa gcggctcccc tacgcacggg aacgggccga acgcgatcga
     3901 atcggttcga tgcgacactc aaggcgggta cggagcaccg ccaccacaat gtccgggacc
     3961 atgcgccgct aggtgaccgg gtagatggtc aacatccgcg ccgggttctc gacgaagatc
     4021 cgccgcaccg acggcgcatc gaacccggcc cccatcagca tcaacggcac ccggtcaatg
     4081 aaatagtcga acccgacggc cgcgccatac ccgaggtggt tcgatttccg cgcaaaatcg
     4141 cccgacacca gcagctggtc gaggtacccc gcatccgcca gccgcaacag tacctccgcc
     4201 cgctcgtcgt ccgatgcgag ctgcaccctc gtccaccggt cgaacgagat gaacgcgcct
     4261 gtcttcagca cctcgatcag gtactccgtc tcgtgcagcc ggtgatccat atggcagagt
     4321 gtgatccggc ccggatctgc cccggccgcc gccatgatct cgatctgttc cagcgccatc
     4381 gttccctgct cggtgtgcgt cgagatcggc gccccgaacg ccacctgcgt cctcgcagcc
     4441 gcctcgagca cccgccgctc gacatccgtg atctcgttca gactcgtccc ggccttgatc
     4501 agcccggccc ggacatccgt cccgtcgatt ccctcgcgca gctcccgcag gttccgctcg
     4561 gtgatcgcct cgactgaatc gttcccgaca cacgggacgg caatcaggtg cttgtggtgc
     4621 cccgtcacca tcacgatgtt gaccggcgcg tgctgcgcga tctgcagcat cgcctcgacc
     4681 gaccggccgt aatccgccgg tcccatgtcg acgatcgacc gtcccccaat cgcaaaatag
     4741 acatcgagat cgagccgcga attggcgatg tcgtccagca ccagatcgtc gtcgccggcc
     4801 gcgagcgggt tggtcaggtt gaagacgtgc tcgtggtgca atgtgaagcc cagcgcgccg
     4861 ggatcgatcg ggccgagtac ggtcatcacg tgcggctggc tcagatcgat ctcttcgtta
     4921 ttgagtggct cgaagctctc c
//
LOCUS       DASSGX010000226         3638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_370015, whole genome shotgun sequence.
ACCESSION   DASSGX010000226 DASSGX010000000
VERSION     DASSGX010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370015"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1334
                     /locus_tag="VFI12_03955"
     CDS             <1..1334
                     /locus_tag="VFI12_03955"
                     /inference="COORDINATES: protein motif:HMM:NF016859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055590.1"
                     /translation="QGRLISMGRNMSVVVTDGDGNERAVHKIPYGAQLRVDEGDKIKR
                     GQRLAEWDPYTRPILTEVDGTIDFEDLVEGASMREQTDEVKGTTNRVVIDWRASARGA
                     DLKPAIVIKDENGKPIKVTRGGDARYLLPVDAILSVEPGAPVKAGDVLARIPMASARS
                     GDITGGLPRVAELFEARRPKDHAIIAEISGTVEFGKDYKNKQRIAIRPDDEDQPSVEY
                     LIPRGKSLAVQHGDRIERGDFVYDGHPAPHDILAIKGVEELANYLINEIQDVYRLQGV
                     TINDKHIEVIVRQMLQKVEVTDSGDTTFIKGEQIDRIELDEVNAKVEKEGGRPATAQP
                     ILLGITKASLQTRSFISAASFQETTRVLTDAAVNGKVDTLEGLKENVIVGRLIPAGTG
                     GMLRRLRRIAAKRDDLIARERAKADAERALAGPELEMPRERRRRRAEEAAE"
     gene            2001..2372
                     /gene="rpsL"
                     /locus_tag="VFI12_03960"
     CDS             2001..2372
                     /gene="rpsL"
                     /locus_tag="VFI12_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HET7055591.1"
                     /translation="MPTIQQLIRKPRKQKVYREKSRHMEGCPQKRGVCTRVYTTTPKK
                     PNSALRKVAKIRLTNGHEVIGYIPGEGHNLQEHSVVLIRGGRVKDLPGVRYHILRGVL
                     DTQGVADRKQRRSKYGAKRPK"
     gene            2489..2959
                     /gene="rpsG"
                     /locus_tag="VFI12_03965"
     CDS             2489..2959
                     /gene="rpsG"
                     /locus_tag="VFI12_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HET7055592.1"
                     /translation="MSRRHRAEKREINPDPKYGDLVITKFMNAVMNQGKKSVAERIVY
                     GALERMESRAKQDPVQMFRQALDNVMPAVEVRSRRVGGATYQVPVEVRNERRQALAIR
                     WLITAARARNENTMVERLSGELLDAANNRGTAVKKREDTHRMAEANRAFSHYRW"
     gene            2981..>3638
                     /locus_tag="VFI12_03970"
     CDS             2981..>3638
                     /locus_tag="VFI12_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET7055593.1"
                     /translation="MPRSHDIGDYRNFGIMAHIDAGKTTTTERILYYTGKSYKIGETH
                     EGSATMDFMEQEQERGITITSAATTCFWLGKRLNIIDTPGHVDFTIEVERSLRVLDGA
                     VCVLDSNQGVEPQTETVWRQGDKYNVPRIIFANKMDKTGADFYMCVEDIKAKLGARPV
                     PVQIPIGAESNFKGVVDLIRMKAMVWEGDGKDAKMVEQDIPADLADRAAEYRAAMIET
                     A"
BASE COUNT          733 a         1122 c         1232 g          551 t
ORIGIN      
        1 gccagggccg gctcatctcc atgggccgca acatgtcggt ggtggtgacg gatggcgacg
       61 gtaatgagcg ggccgtgcac aagatcccgt acggcgcgca gctccgggtc gacgagggcg
      121 acaagatcaa gcgcggtcag cggctcgcgg agtgggatcc ctacacccgc ccgatcctca
      181 ccgaggtcga tggtacgatc gacttcgagg acttggtgga aggggcctcg atgcgcgagc
      241 agacggacga ggtgaagggc acgaccaacc gcgtcgtcat cgactggcgc gcctcggcgc
      301 gtggcgccga tctgaagccg gccatcgtca tcaaggacga gaacggcaag cccatcaagg
      361 tgacccgcgg tggcgacgcc cgctacctgc tgccggtcga cgcgatcctc tccgtcgagc
      421 ccggtgcgcc ggtcaaggcg ggtgacgtgc ttgctcgtat cccgatggcg tcggcccgct
      481 cgggcgacat cacgggcggt ctgccacgtg tcgcggagct gttcgaggcc cggcggccga
      541 aggaccacgc gatcattgcg gagatctcgg gcacggtcga gttcggcaag gactacaaga
      601 acaagcaacg gatcgcgatc cggcccgacg acgaggatca gccgtcggtc gagtatctga
      661 tcccgcgtgg caagagcctt gccgtgcagc acggggaccg catcgagcgc ggcgacttcg
      721 tctacgacgg ccacccggca ccgcacgaca ttctggcgat caagggcgtc gaggaattgg
      781 cgaactacct gatcaacgag atccaggacg tctaccggct gcagggcgtg accatcaacg
      841 acaagcacat cgaggtgatc gttcgccaga tgctgcagaa ggtcgaggtc accgattccg
      901 gcgacacgac cttcatcaag ggcgagcaga tcgaccgcat cgagctcgac gaggtgaatg
      961 cgaaggtcga gaaggagggc ggccggccgg cgacggcgca gccgatcctg ctcggcatca
     1021 ccaaggcctc gctgcagacc cgctcgttca tctcggcggc ctccttccag gagaccacgc
     1081 gcgtgctgac cgatgccgcc gtcaacggca aggtcgatac actcgagggg ctgaaggaga
     1141 acgtgatcgt cggccggctc attccggcgg gcacgggtgg catgctgcgc cgcctgcgcc
     1201 ggatcgcggc caagcgcgac gacctcatag cccgcgagcg ggcgaaggcc gacgcggagc
     1261 gggctctggc cgggccggag ctcgagatgc cgcgcgagcg gcgccgccgg cgcgccgagg
     1321 aggcggccga gtaagcaagg ccgccgagat ttcggaacgg aacggctgcc ttcgggcagc
     1381 cgttctcgtt tgccaagcaa aaagcgacag tcaatcaaga caatgcgaag atgaaccggg
     1441 gatcagcggg aacgtgcacg gcggcgcagt attgaggtga gccgaactcg aagctcctga
     1501 tctggagtct ccggcatcgc gccccagaca ctggccagtc ctgcgggcca ccgctccttt
     1561 ccgcccggct gtctggcagg cccatgctgc cccttccaaa tgcgagcaag tctcgatggt
     1621 tcggctatga gcgtagcaga tgatggggct gcggaccgtc gcggggccct tagtcggcag
     1681 aaccgattgt gccgtcgaga aaaacccgat ttgcgcggct caatactgtt gacctgagat
     1741 gagaccatgt atatggtccg cgcactctca cggtaggtgg taggcctcat cgggcgctgg
     1801 atccgtgttt cgggccctgg cttagacgct gccgtagcaa agcagagcac aacttcggcc
     1861 catgatcccc ggtccgggtg gccgcggatc ctctggtttc gtcgtacccg tcacactccg
     1921 gggatggaag gtggctggtg cggcttggcg cttcccgcgt ctggctatgc gggaagctct
     1981 ggtggaatgg aagaaggcgc atgccgacca tacaacagct gatccggaag ccgcggaagc
     2041 agaaggttta ccgcgagaag tcgcgccaca tggagggctg cccgcagaag cgcggcgtct
     2101 gcacccgtgt ctacacgacg actccgaaga agccgaactc ggcgctgcgc aaggtggcga
     2161 agatccggct taccaacggc cacgaggtga tcgggtacat cccgggcgag ggccacaacc
     2221 tgcaggagca ctcggtggtg ctgatccgcg gcggccgcgt caaggacctg ccgggcgtgc
     2281 gctatcacat ccttcgcggc gtgctcgaca cgcagggcgt cgcggaccgc aagcagcgcc
     2341 gctccaagta cggcgccaag cggccgaagt gatctgaagg cgaattggga cgttcatccc
     2401 ggacggggcg aaatttcaag tccagacggg atgctcgagg tgaaaatggg ccgctccagc
     2461 ggctcgagcg aggacaggaa cgagacgcat gtcgcgccgc cacagagccg agaaacgcga
     2521 gatcaaccct gatccgaagt acggcgatct ggtgatcacc aagttcatga acgccgtcat
     2581 gaatcagggc aagaagtcgg ttgccgagcg tatcgtttac ggcgccctcg agcggatgga
     2641 gagccgggcc aagcaggacc cggtgcagat gttccgtcag gcgctcgaca acgtaatgcc
     2701 ggctgtcgag gtccgctccc gccgtgtggg tggtgcgacc tatcaggtcc ccgtcgaggt
     2761 ccgcaacgag cggcggcagg cactggcgat ccgctggctc atcacggccg cgcgggcacg
     2821 caatgagaat acgatggttg agcgactctc aggcgagctg ctcgacgccg cgaacaaccg
     2881 tggaacggcg gtgaagaagc gcgaggacac gcacaggatg gccgaggcca accgggcttt
     2941 ctcgcattac cgctggtgac gaatttcgga gactgagcca atgccgcgct cacatgatat
     3001 tggggattac cgcaacttcg gcatcatggc ccacatcgat gccgggaaga cgacgacgac
     3061 cgagcggatc ctgtattaca caggcaagtc ctacaagatc ggtgagaccc acgagggctc
     3121 cgcgaccatg gacttcatgg agcaggagca ggagcgtggc atcacgatca cctcggcggc
     3181 gacgacctgc ttctggctgg gcaagcgcct gaacatcatc gacaccccgg gccacgtcga
     3241 cttcaccatc gaggtggagc gcagcctgcg cgtgctcgac ggcgccgtgt gcgtgctcga
     3301 ctccaaccag ggcgtagagc cgcagacgga gacggtctgg cgccagggcg acaaatacaa
     3361 tgtgccccgc atcatcttcg ccaacaaaat ggataagacc ggcgctgact tctacatgtg
     3421 cgtcgaggac atcaaggcaa agctcggcgc ccggcctgtg cccgttcaaa ttccgatcgg
     3481 cgccgagagc aacttcaagg gcgtcgtgga cctcatccgc atgaaggcga tggtctggga
     3541 gggtgacggc aaggacgcca agatggtcga gcaggacatc cctgccgatc tggccgacag
     3601 ggccgccgag taccgggccg ccatgatcga aacggccg
//
LOCUS       DASSGX010000227         4956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1240459, whole genome shotgun sequence.
ACCESSION   DASSGX010000227 DASSGX010000000
VERSION     DASSGX010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4956
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240459"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VFI12_03975"
     CDS             complement(<1..252)
                     /locus_tag="VFI12_03975"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HET7055594.1"
                     /translation="MGPEITATDVQSPIRKTGWHDQRADDAFTPGVKHGQILIMFALM
                     LTALLGAVGLSVDLGMAFSQRRTMQSAADAGAYAGAWAVL"
     gene            complement(515..1567)
                     /locus_tag="VFI12_03980"
     CDS             complement(515..1567)
                     /locus_tag="VFI12_03980"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HET7055595.1"
                     /translation="MGRTVSTRRKTEHCDTGPTGGDRVHRGQILVMFAFMLVAMLGAV
                     GLAVDLGMAFSQRRTVQAAVDGGAYAGARVIARSNPAAPSSAMAEVTTIVNANMMNYG
                     AISSLTCKYVNDGGSEIGDCSAAVPPAGTGVRVAVSETHNTFFIKVVPGGPKTVTTSA
                     SARANIRIITGFSDGPFLPCAKKAQLANGGQMDIWDDVNNKVNDAAWGTEFKIHGPQV
                     EKCDIKPSKYKGLADQDANAGLAAPGWFHFNQGDKAGPARFDVQGADGCKAGQDPKNC
                     VMFLPIITNKGQPVPSSSTDAWVVALIPFYITEPKSNEHNGIPIKNYVVAGNGQDGQG
                     GWTPYWKGPIAIRMTE"
     gene            2054..3163
                     /locus_tag="VFI12_03985"
     CDS             2054..3163
                     /locus_tag="VFI12_03985"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055596.1"
                     /translation="MKSTTGTATGHGSPNGGSDPAEAREVGLRFSTDADAGIQRRKHG
                     QGFRFVDADGAAVTDEATLQRIAALAIPPAWTDVWICSRANGHLQATGRDARGRKQYR
                     YHSRWRAHRDETKFGRMAEFGRVLPAIRARVEEDLHRHGLPREKVLAAVIALLERTRI
                     RVGNDEYARANKSFGLTTLRDRHASIGSTSLRFHFRGKSGKEHEVTLQDRRLARIVKR
                     CQDIPGQRLFQYEDDDGDRHHVGSAEVNDYLREIAGDDYTAKDFRTWAGTMIAAGALR
                     HTPKPETDADAGRDVLAAIDAVADDLGNTRAVARASYIHPAIFEAYRDGSLQAIDPDG
                     IDDPEPGGLDDDEKVVLAVLSRAGAGREGRKARRR"
     gene            complement(3333..4349)
                     /locus_tag="VFI12_03990"
     CDS             complement(3333..4349)
                     /locus_tag="VFI12_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055597.1"
                     /translation="MLLIRIAIALVLTLGVLGLAASPSLSAPGFAKDKHGQGHNGKSP
                     KKDRHDNGKHKSQKKDKDKHDNGKHKGKNKGEKRVTAEQIATYTVRVDCSYDAADDET
                     TCTVDADHPEGGKKINLFVLSADDICAPVVRTDANYVDPDPNTGATGYRSTDSEGKLL
                     LVLSGKVIAGGTATDWIKAASNIFPATGTGLVCATDEPAQQSAPTQPATDVTPAPTET
                     QAPVTPEVTDTSGSVVVQAYDCPIATPRADYDWYGQCASATSGSRYRLTRTDTETRDG
                     LTTSTDAMGRATFRSLPPGTYQLTQAEGDWCFAQSDNVNSDGSLTVSAGQRTSVWVFD
                     CAGT"
     gene            4590..>4956
                     /locus_tag="VFI12_03995"
     CDS             4590..>4956
                     /locus_tag="VFI12_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055598.1"
                     /translation="MESDRAAARAKSVIARHPAGVFEHDCALPATPTALVGRETDLVR
                     ASRLIGQSSVRLFTVTGPGGTGKTRFAIALATELTPSYDLGAVFVDLTPLNEPALVLP
                     EIGLALGVRESAARSIAAGI"
BASE COUNT          861 a         1533 c         1646 g          916 t
ORIGIN      
        1 cagcactgcc cacgcgccgg cgtaggcgcc ggcgtcggcc gcgctctgca tcgttcggcg
       61 ttgcgaaaag gccatgccca ggtcgaccga gaggccgacc gcgccgagga gcgccgtgag
      121 catcagcgcg aacatgatca agatctgtcc atgcttgacg cccggcgtga aggcgtcgtc
      181 agcgcgctgg tcgtgccagc ccgttttacg gatgggcgat tgaacgtctg tggctgtgat
      241 ttcgggtccc atgcggccca gactccttac tcgtgccagg tggaccgggg tccgtacccg
      301 gccgtagcca gatcgtaccg agaggacatc cgggagtact atccggggtc atagcccgga
      361 agggggagcc cgggaaagta ctgaggtacc gaagggcgtt ggtactgttc ggggtgcgtg
      421 gaatcagcag gcgaggggcg ccacgaacgt agaccggccc tgccgtactg gcagggccgg
      481 atcaattgat cacactgctc tttgggactc gagtctactc ggtcattcgg atcgcaatgg
      541 ggcccttcca gtagggggtc catccgcctt gaccgtcctg gccgttgcca gcgacgacgt
      601 agttcttgat cgggatgccg ttatgctcgt tcgacttggg ttcggtgatg tagaagggaa
      661 tcagcgccac gacccaggcg tcggttgacg aggacgggac tggctgcccc ttgttggtga
      721 tgatcggcag gaacatcacg cagtttttcg ggtcttggcc ggccttgcag ccgtcggctc
      781 cctgaacgtc gaaccgagcc gggccggcct tgtcgccctg attgaaatgg aaccagccag
      841 gcgccgccag gccggcattc gcgtcctggt cggccagccc cttgtacttg ctcggcttga
      901 tgtcgcactt ttcgacctgg ggaccgtgga tcttgaattc cgtgccccag gctgcgtcat
      961 tgaccttgtt gttgacatcg tcccagatat ccatttgccc gccattggca agctgggcct
     1021 tcttggcgca cggcaggaac ggtccgtccg agaagccggt aatgatgcgg atattggccc
     1081 gggcgctggc tgacgtagtc actgtcttcg gcccgcccgg gacgaccttg atgaagaacg
     1141 tgttatgcgt ttccgaaact gcgactctga caccggttcc ggccggcgga acagcagccg
     1201 agcagtctcc gatctcgctg ccgccgtcgt tgacgtactt gcaggtcagg gatgaaatcg
     1261 cgccgtagtt catcatgttg gcgttaacga tggtcgtcac ttcggccatg gccgacgatg
     1321 gagccgcggg gttcgaacga gcgataaccc gtgcgccggc atacgcgccc ccgtcgactg
     1381 cggcctgcac cgtccggcgc tgagaaaatg ccatgccaag gtcgaccgcc agaccgacgg
     1441 ccccgagcat ggcaacgagc atgaacgcga acatgaccag gatttgcccg cgatgcactc
     1501 ggtcgccgcc ggtcggtccc gtgtcgcagt gctctgtttt tcgccgggtc gaaacagttc
     1561 tgcccatttc gttttcctcc gactacaagc aatacatggc acggtcaacc gacagtacgg
     1621 gctggagtct caagccgagc acgacagcga cccaatgagt gtcatccccc gaacggccca
     1681 ggaccaagca tcatgagtag ccctgtcagg gggtttgccc caagaccacg gtaccggcat
     1741 ccctgctacg tattggccgt ccccgcacga cccgcgtcac aagcgcggcg ctcaaaacac
     1801 acagtgcgac cgaggtcagg aacgattaac gcttgtatat cgagttccgc ggagaagcag
     1861 aaggcattct gatagtctcg agtaccaaag cgatagagtg gtcagtactg tacgaacaag
     1921 tactgtcgga tgagaggcga ggtacgcgag ccggtggcag cggcgcgacc cgcagtcgat
     1981 ccgtatcgcg cgcaccggcg aatcggcgcc accgccgcaa aatgctcgag gtagttcggt
     2041 cgccaggatc acgatgaaat caacaaccgg gacagcgaca ggtcacgggt cgccaaatgg
     2101 tgggtccgat ccggcggagg ctcgcgaggt cggcctccgt ttttcgacgg acgcggatgc
     2161 aggcatccag cgccggaagc acggccaggg ctttcggttc gtagacgcgg acggcgcggc
     2221 cgtcaccgac gaggcgacgc tgcagcggat cgcggcgctc gcgatcccgc cggcctggac
     2281 tgacgtctgg atttgcagcc gcgccaacgg gcatctccag gcgaccggcc gggatgcgcg
     2341 cgggcgcaag caatatcgct accactccag gtggcgagca catcgtgacg agacgaagtt
     2401 cggtcgaatg gcggaattcg gccgtgtgct cccggcgatc cgggccaggg ttgaggagga
     2461 tctgcaccgg cacggcctgc cgcgggagaa agtgctggcc gcggtgattg cgctgctgga
     2521 gcggactcgg atccgggttg gcaacgacga atatgcccgg gcgaataagt cgttcgggct
     2581 gaccaccttg cgtgatcgcc atgcctccat tggctcgacg tctctccgct tccacttccg
     2641 cggcaagtcc ggcaaggagc atgaggtgac gctccaggat cgccggctgg cgcgcatagt
     2701 gaaacgctgc caggacatcc ccgggcagcg cttgttccag tatgaggatg acgacggcga
     2761 ccggcaccac gtcggctcgg ccgaggtcaa cgactacctc cgggagatcg ccggggacga
     2821 ctacaccgcc aaagacttcc gcacctgggc cggcacgatg atcgcggccg gcgcgctccg
     2881 tcacaccccg aaacctgaga ccgatgcgga cgccggccgg gacgtgctgg cggcgatcga
     2941 cgcagtcgcg gacgacctcg gcaatacccg cgcggttgcc cgggcgtcct acattcaccc
     3001 ggcgatcttc gaggcgtacc gggatggatc actccaggct atcgacccgg atggcatcga
     3061 cgatccggaa cccggcggcc ttgatgacga cgagaaggtg gtgttggcgg tgctgagtcg
     3121 agcaggcgcg gggcgcgaag gtcggaaggc gaggaggcgc tagctcatgt tggggcagat
     3181 ggcatctgcc cgcctcgcgc ccaccgcgcc gctggcatac atcgacccgg taccggccgc
     3241 ggaaccgaaa acgtccgttg cggatgagcg cgggcagatg ccatctgccc ttacaacagg
     3301 tgcctcaact ctcgcaaccg tccaccctca acttacgtcc cggcgcagtc gaagacccag
     3361 actgacgtgc gctgacccgc ggaaacggtc aggctgccat cgctgttgac gttgtcggac
     3421 tgcgcgaagc accaatcacc ttcggcttgg gtcaattgat aggtgccggg cgggagtgac
     3481 cggaacgttg cccggcccat ggcgtcggtc gaggttgtca ggccgtcgcg tgtttcggtg
     3541 tccgtgcgcg tcagccggta gcgcgaaccg gaggtcgccg aggcgcactg cccgtaccag
     3601 tcatagtcgg ctcggggcgt cgcaatcggg cagtcgtagg cttggacgac cacggagccc
     3661 gatgtatcag tgacctccgg cgtaacggga gcctgtgtct ccgtgggagc cggcgtcacg
     3721 tcggtggccg gctgggtcgg agcggactgc tgcgctggct cgtcggtcgc gcagaccagg
     3781 ccggttccgg tggcggggaa gatgttgctg gcagccttta tccaatctgt ggcagtgcct
     3841 cccgctatca ctttcccgga taggacgagc aggagcttgc cttcggagtc ggtcgagcga
     3901 taaccggttg cgccggtatt tggatccggg tcgacatagt tcgcatcggt gcggacgacg
     3961 ggcgcgcaaa tgtcgtcggc ggacagtacg aacaggttga tcttcttgcc gccctcggga
     4021 tgatccgcat cgacggtgca ggtcgtctcg tcgtcggctg cgtcatagga gcagtcgacc
     4081 cgcacggtgt aggtggcaat ctgttccgcc gtgacgcgct tctcgccttt gtttttccct
     4141 ttgtgtttcc cgttatcatg cttgtccttg tcctttttct ggctcttgtg ctttccgttg
     4201 tcgtgcctgt ccttcttggg ggattttccg ttgtggccct gcccgtgctt atccttggcg
     4261 aagcccgggg ccgacagcga tggggaggca gccaggccca gcacgccgag cgtcagcacg
     4321 agtgcgatgg cgatccgaat caaaagcatg gggcggggca cgtgcatgaa tacctcccgg
     4381 ggaacggcgt gcttcgcgac ctcctccgtg acagattcgg tcacgtccga agtacgaacc
     4441 tgaagataca cggatggccc cgacgcctct gtagcccggg acgcaccgat cccgtcgata
     4501 gcgcggcctt cttctctgca actcacaatt agtggatact tgtggccgtt caccgcaaat
     4561 gcagcctctg gacccgggga gatgagttcg tggagtcaga tcgcgcggcc gcgcgcgcga
     4621 aatcggtgat cgcaaggcat ccggcaggag tcttcgagca cgactgcgcc ttgccggcga
     4681 caccgacggc cctggtgggc cgggagacgg accttgtccg ggcgagccgg ctgatcgggc
     4741 agtcgagcgt gcggctgttc accgtgaccg gcccgggcgg aacgggcaag acccggtttg
     4801 cgatcgcgct ggccactgaa ctgacgccgt catacgatct cggcgcggtc ttcgtcgatc
     4861 tcacgccgct gaatgagccg gcattggtgc tccccgagat tgggctagcg ctcggcgtgc
     4921 gcgagtcggc tgcgcggtcg atcgccgccg gcatcc
//
LOCUS       DASSGX010000228         5985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_151417, whole genome shotgun sequence.
ACCESSION   DASSGX010000228 DASSGX010000000
VERSION     DASSGX010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5985
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151417"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            427..1773
                     /locus_tag="VFI12_04000"
     CDS             427..1773
                     /locus_tag="VFI12_04000"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET7055599.1"
                     /translation="MLLLPMMLALTLGGSVRAQTPAASPVVNTSVSGELNVAMVANPQ
                     MVALQKLAPEFQKMYPNIKLNLLVLPENEVRDRIRTDVSTQAGQFDVVTIGMFEAADY
                     AKQGWSLDVGTPLQADPNYDYQDIFPAMVSALSGDDGKLYAAPFYGESSMVFYRKDLF
                     EAAGLTMPENPTWDDIQGFAQKLHKPDSGQYGICLRGLPGWGEQGAPLTSVINAFGGR
                     WFDNDWKSQLASDKSAKAITFYTDLVKNYGEPSATTAGFTECENLFIQGKVAMWYDAT
                     SAADLLADPKQNPQAANTGFAYQPKEEIQSGWLWAWAFAIEAKTKHQDAALAFVKWAT
                     SKDYVQLVAKQLGWGSVPSGVRASTYADPGYKEYGAAFADLVLSIIQAQDPTKCCVDQ
                     VPYKGLQFVGVPEFVTLGDQVTKELANVIAGNESAADAIKKIDDMANTWAQDNGYQ"
     gene            1851..2801
                     /locus_tag="VFI12_04005"
     CDS             1851..2801
                     /locus_tag="VFI12_04005"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET7055600.1"
                     /translation="MAKEDLAGARRAAAAREPSRDYGSIGRWLVAPALIYAIIVTQAP
                     FIYTLYYSTFRYNLLRPERRKFIGIQNYQRILTSDTVFRDALTNTFIFTFAAVVISLL
                     LGLFYAELVNHRFPGRGIVRTMLITPFLVMPVAGALGWKNMMMDPDFGVIDWLLRAIQ
                     PGTNSWNPVWLAHYPRQSIIIMLVWRWAPFMMLILLAGMQSIDEEVREAARVDGASPV
                     QEFFGITLPHLYRFMQLGALLGAVYIVQEVDQIFMTTQGGPGTLTTTLPYYVYETAIR
                     GSQVGRGAAVGVAVVILTIIGMTLLLRLLDRMLRSQYADG"
     gene            2788..3696
                     /locus_tag="VFI12_04010"
     CDS             2788..3696
                     /locus_tag="VFI12_04010"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET7055601.1"
                     /translation="MQTARPFPFRRHASGARKESAPLAWAILAWVVGLIVFFPAFYIV
                     ATGFKSEATAVQLPPKFIPLPSEISLGWLNRFIFHPTLEQYQEVRSRGFWPFFRNSII
                     ASLVSTAIVLILAIPAAYALALRPMKRWKDILFFFISTKFLPPAGVIIPLYIIFNNLH
                     LLNTIQGLVIMYVAMNLPIAIWMLRSFFEEVPRDILDAARVDGAGIFREMVEILMPMV
                     GPGIAATAFLSVIFAWNEFFFAVNLVATEKAATVPLFMQKFITSEGLFWAKLSAASTM
                     AIAPVVILGWISQRQLVRGLSMGAVK"
     gene            3805..4812
                     /locus_tag="VFI12_04015"
     CDS             3805..4812
                     /locus_tag="VFI12_04015"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HET7055602.1"
                     /translation="MKAVVVEKPGSVRVENVPEPVSGPKDVIVQVGACGICGTDIHII
                     DGEFPPTSYPIIIGHEFGGTVVDVGDQVVGVKVGDRVGVDPTLNCGECYFCQRGQGNL
                     CERWNAVGVGAHPGGFAECVAVPERAVYPIPEGMSFAAAALIEPVSCVVHGFHLINPR
                     PGDSYLIYGAGPMGLQNAQVARFSGAREVAIIDINPDRLEVARSFGFETLGQSLDDVK
                     GAAPRGFDIVIEATGKTKVAEIAINAVIRGGKLLLFGVCPPGEKAAYEPFKIYNEELT
                     IVGSMAVLRSYGPAVDLIHAGAIDTEKMVTHTFPIDQFAEAVELVRKGGGLKVQIDPS
                     A"
     gene            5171..>5985
                     /locus_tag="VFI12_04020"
     CDS             5171..>5985
                     /locus_tag="VFI12_04020"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET7055603.1"
                     /translation="MRQIVLERPQSIVMRDVPTPTPRPGDALVRSEQVGICGSDLHAY
                     HGKHPFITLPVVPGHEVVGTVEALGEGATGVKVGDRVVLEPNIVCGECVHCRSGRYNL
                     CEKLTVIGCVGPLDGAMGDYFIAPAERLIPIDSRLSLTQAAMIEPLATGTHAVRVADG
                     TQGKRVAVLGSGSIGLLTMQAARAGGAATIVVTDLSPAKRQRALDLGANAAFDPRDPN
                     VVDAMREKLGGPADVVFDCVAIQTTMDQAIALAHKGGTVVVEGVPEADVTVPLA"
BASE COUNT         1013 a         2005 c         1926 g         1041 t
ORIGIN      
        1 gcgggcagat gccatctgcc cctacagatt tggatgcgag gccggccgga gcccgccgtc
       61 ggctaatggg gccgcgtaac ctgggcccct attcccccaa ccctccaccc taaaccctca
      121 accaacccgt agtgactgcg cggcactctg tgttgcatac cttccgctac aggcatattg
      181 tgtgcgcagg tgtcctcttt ttgcgtgact ttgcggccca ggcggcgaat tcgtgtcccc
      241 gccggacggc agccgggaac ggtgggcttt gggggccgtt cctcgcgcgg gcaatgaagg
      301 aggtgtggcg gaagcagtcg acgtgaccca gcgactcaat tgagcgcagt caggtactgc
      361 ggcgtcccgt acgccgaaag gagacagcag atgcaacgga gattccgaac ggttcttgcg
      421 gttctcatgc tgttgttacc aatgatgctg gcactgaccc tgggcggctc cgtccgggcc
      481 caaacccccg ccgcgtcgcc cgtcgtcaac acctcggtct ccggcgagct taacgtggcc
      541 atggtcgcga acccgcagat ggtcgcgctg cagaagctcg cgcccgagtt ccagaagatg
      601 taccccaaca tcaagctcaa cctcctggtc ctccccgaga atgaggtccg cgaccgcatc
      661 cggaccgacg tctcgaccca ggccgggcag ttcgatgtcg tcacgatcgg catgttcgag
      721 gcggctgact atgccaagca gggctggtcc ctcgatgtcg gcacgcccct ccaggccgat
      781 cccaattacg actaccagga catcttcccg gctatggtct cggccctctc cggcgacgac
      841 ggcaagctct acgccgcccc gttctacggc gagagctcga tggtcttcta ccgcaaggac
      901 ctcttcgagg ccgcggggct gaccatgccc gaaaatccga cctgggacga catccagggg
      961 ttcgcccaga agctgcacaa gccggactcc ggccagtacg gcatctgtct gcgcggtctc
     1021 cccgggtggg gcgagcaggg cgcgccgctg accagtgtca tcaacgcctt cggtggccgc
     1081 tggttcgata acgactggaa gtcgcagctc gcctccgaca agtccgcgaa ggcgatcacc
     1141 ttctacaccg acctggtcaa gaactacggc gagcccagcg ccaccacggc cggcttcacc
     1201 gagtgcgaga acctcttcat ccagggcaag gtggcgatgt ggtacgacgc gacctccgcg
     1261 gccgaccttc tggctgatcc gaagcagaac ccgcaggccg ccaacaccgg cttcgcctac
     1321 cagccgaagg aagagatcca gagcggctgg ctgtgggcct gggcgttcgc catcgaagcg
     1381 aagaccaagc accaggacgc cgccctcgcc ttcgtgaagt gggcgacgtc gaaggactac
     1441 gtccagctcg tcgccaagca gctcggctgg ggttcggtgc cctccggcgt ccgcgcctcg
     1501 acctatgccg atcccgggta caaggagtac ggcgcggcgt tcgctgatct cgtgctcagc
     1561 atcatccagg cccaggatcc gaccaagtgc tgcgtcgatc aggtgcccta taaggggttg
     1621 cagtttgtcg gggtgccgga gttcgtcacc ctcggcgacc aggtaacgaa ggagttggct
     1681 aacgtcatcg ccggcaatga gagcgcggcc gacgcgatca agaagatcga cgatatggcc
     1741 aacacctggg cacaggacaa cggctaccag tagcacgcgc catccgggag tcgggcggga
     1801 tcccgcccga ctcccaaccc ggacgatcct aatcgaggtg atctatggcc gtggcgaaag
     1861 aggaccttgc cggcgcccgc cgggctgccg ctgccaggga gccgtcccgt gactacggct
     1921 cgatcggccg ctggctggtg gcgccggcgc tgatctacgc gatcatcgtc acccaggcgc
     1981 ccttcatcta cacgctctac tacagcacct tccgctacaa cctcctccgt cccgaacggc
     2041 ggaagttcat cggcatccag aactaccagc gcatcctgac cagcgacacg gtcttccgcg
     2101 atgcgctcac taataccttc atcttcacct tcgccgcagt cgtcatctcg cttctgctcg
     2161 ggctcttcta cgcggagctg gtcaaccacc gtttcccggg ccggggcatc gtccgcacca
     2221 tgctgatcac gcccttcctg gtgatgccgg tggccggcgc gctgggctgg aagaacatga
     2281 tgatggaccc ggatttcggg gtcatcgact ggctgctccg ggcgatccag cccgggacca
     2341 attcatggaa cccggtctgg ctggcccatt acccgcgcca atcgatcatc atcatgctcg
     2401 tctggcgctg ggcgcccttc atgatgctga tcctgctggc cgggatgcag tcgatcgacg
     2461 aagaggtgcg cgaagccgcc cgggtcgatg gcgcatcgcc cgtccaggag ttcttcggca
     2521 tcacgctgcc gcacctctac cgcttcatgc agctcggcgc gctgctcggc gcggtctaca
     2581 tcgtccagga ggtcgaccag atcttcatga cgacacaggg cggtcccggc acgctgacaa
     2641 ccacgctgcc ctattacgtc tacgagacgg cgatccgggg aagccaggtc gggcgcggcg
     2701 cggcggtcgg tgtggcggtc gtgatcctga cgatcattgg catgaccctc ttgctgcgcc
     2761 tgctcgatcg catgctgagg agccaatatg cagacggcta gacccttccc gttccgcaga
     2821 cacgcgagcg gcgcgcgcaa ggaaagcgcg ccccttgcct gggcgatcct tgcctgggtg
     2881 gttgggctga tcgtcttctt cccggctttc tacatcgtcg cgaccgggtt caagtcggag
     2941 gcgacggccg tccagctgcc gcccaagttc atcccgctgc cgagcgagat cagtctcggc
     3001 tggctcaacc gcttcatctt ccacccgacg ctggagcagt accaggaggt gcggagccgg
     3061 ggcttctggc ccttcttccg caactcaatc atcgcctcgc tggtctcgac ggcaatcgtc
     3121 ctgatcctgg cgattccggc cgcctacgcg ctggcgctgc ggccgatgaa aaggtggaag
     3181 gacatcctct tcttcttcat ctcgaccaag ttcctgccgc cggccggcgt gatcatcccg
     3241 ctctacatca tcttcaacaa cctccatctc ctgaacacga tccagggcct ggtcatcatg
     3301 tacgtcgcga tgaacctgcc gatcgcgatc tggatgctgc gctccttctt cgaggaggtc
     3361 ccgcgcgaca tcctggacgc cgcccgcgtc gacggcgccg ggatctttcg agagatggtc
     3421 gagatcctga tgccgatggt cggccccggg atcgccgcga ccgccttcct gtccgtgatc
     3481 ttcgcgtgga acgagttctt ctttgcggtc aatctggtcg ccacggagaa ggcggcgacg
     3541 gtgccgctct tcatgcagaa gttcatcacc tcggaaggtc tgttctgggc caaactatct
     3601 gccgcgagta cgatggcaat agcgccggtc gtcatcctcg gctggatctc gcagcgccag
     3661 ctcgtccgcg gcctgtcgat gggagcggtg aaatgaggcg agaagcaggg cgtggaggta
     3721 acggggtgat gggatggggc cctcgcccga aaaccgaaaa ccgagccccg aaaaccgagg
     3781 acccaaaacc tcaggagtcg tgtcatgaaa gcagtcgtcg ttgagaagcc cggttcggtc
     3841 cgggttgaga acgtgccgga gccggtgtcc gggccgaagg acgtgatcgt ccaggtcggc
     3901 gcctgcggca tctgcggcac cgatatccac atcatcgatg gtgagttccc gccaaccagc
     3961 tacccgatca tcatcgggca cgagttcggc ggcaccgtcg tcgacgtcgg cgatcaggtc
     4021 gttggcgtca aggtcggcga tcgggtcggg gtcgatccga cgctgaactg cggggagtgc
     4081 tacttctgcc agcgcgggca gggcaacctc tgcgagcgct ggaacgcggt cggcgtcggc
     4141 gcccacccgg gcgggttcgc cgagtgcgtg gccgtgccgg aacgcgccgt ctacccaatc
     4201 ccggagggaa tgtccttcgc cgcggcggcg ctgatcgagc cggtcagttg cgtggtccac
     4261 ggcttccacc tgatcaaccc gcgcccgggc gacagctacc tgatctacgg cgcgggcccg
     4321 atgggcctgc aaaacgcgca ggtggcccgc ttctccgggg cgcgcgaagt cgcgatcatc
     4381 gacatcaacc cggaccggct cgaggtggcg cgctccttcg ggttcgaaac gctcgggcag
     4441 tcgctcgacg acgtgaaggg ggccgcgccg cgcgggttcg acatcgtgat cgaggcgacc
     4501 ggcaagacga aggtggcgga gatcgcgatc aacgcggtga tccggggcgg caagctgctg
     4561 ctcttcggcg tctgcccgcc gggcgagaag gccgcctacg agccgttcaa gatctacaac
     4621 gaggagctga cgatcgtcgg ttccatggcc gtgctgcgca gttacggccc ggccgtagac
     4681 ctgatccacg ccggcgcgat cgacacggag aagatggtca cccacacgtt cccgatcgac
     4741 cagttcgccg aagccgtgga gctggtccgc aagggcggcg gcctgaaggt gcaaatcgac
     4801 ccatcggcgt gacggagtcg agcagccgcg acgacgatgg aggggtttgc gggggcaaac
     4861 ccgccctgcc ccaatcgccg gttgcaattg gagcgagacc gtgtcaaggc agatggcatc
     4921 tgcccgtcgc tgcgcaatcg cccgtcacat gcacccgggc caggtcgtag cgcggtccac
     4981 ccatgggccg catcatgcca cccctgcgcg gtagatgaca ctggcccggc gctggggttg
     5041 gttccccgga acggtcgggc cgtgaccggg cccgggcggc ccacgggggg accgcgctac
     5101 gtttcaggat gtattccggg cttatcaccg ggcgctcttt gccatagccc ggtcatttgg
     5161 aggaatttcg atgcgtcaaa tcgtgctgga gcggccgcaa tcgatcgtga tgcgggatgt
     5221 gccgaccccg accccgcggc cgggcgatgc cctggtccgc agcgagcagg tcggcatctg
     5281 tggctccgat ctccatgcct accacggcaa gcatccgttc atcacgctcc cggtggtgcc
     5341 cggccatgag gtcgtcggca ccgtcgaagc gctcggcgag ggtgccaccg gggtgaaggt
     5401 cggcgaccgg gtcgtcctcg agccgaatat cgtctgcggc gagtgcgtcc actgccgctc
     5461 cggccgctac aacctctgcg agaagctgac ggtcatcggc tgcgtcggcc cgctcgacgg
     5521 cgcgatgggc gactacttca tcgctccggc cgagcgcctg atcccgatcg attcgcgcct
     5581 gagcctgacc caggcggcca tgatcgagcc cctcgcaacc ggtacccatg ccgtgcgcgt
     5641 tgccgacggg acacagggca agcgggtcgc ggtgctcggc agcggctcga tcggcttgct
     5701 gacgatgcag gcggccaggg ccggcggcgc ggcgacgatc gtggtcacgg acctgagccc
     5761 ggccaagcgg cagcgcgcgc tcgacctcgg cgcgaatgcc gcatttgacc cgcgcgaccc
     5821 gaacgtggtc gacgccatgc gtgagaagct cggcggtccg gccgatgtcg tcttcgattg
     5881 cgtcgcaatc cagacgacga tggaccaggc gatcgcgctg gcccacaagg gcggcactgt
     5941 cgtcgtcgag ggcgtgcccg aggcggacgt gaccgtgccg ctcgc
//
LOCUS       DASSGX010000229         2387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_696255, whole genome shotgun sequence.
ACCESSION   DASSGX010000229 DASSGX010000000
VERSION     DASSGX010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696255"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..881
                     /locus_tag="VFI12_04025"
     CDS             96..881
                     /locus_tag="VFI12_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055604.1"
                     /translation="MSPALHGSGQIRFWLCVGLVIGMLGGVALAWRWTAAGTGARVAV
                     LGAGKSVSVLITNDHRRVLIASGSNGTAFSNALGAALPPFVDNIDLVLIDSRASADLI
                     DRVKALDAKRTMMLPDPERDDPAETILRSFQVDLGEGVILSVRIEPAGKWSAELRTSA
                     GILSISPGGVDLAPALVRITLDGTTAVEPGGTPSIWIGPAANGLVRTSHQATVGTGSV
                     LPITMDGSAFRIPREFFGAVESDQRTNHFPSLDGQAIVEFRPD"
     gene            complement(813..2036)
                     /locus_tag="VFI12_04030"
     CDS             complement(813..2036)
                     /locus_tag="VFI12_04030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HET7055605.1"
                     /translation="MDDLQQRIIDRIDREAGALIELSKFIHSHPEIAMQEVQSSAACA
                     KFVEDRGFMVQRGVANLPTAFSATVGAGAGGHVGYLSEYDALAGLGHGCGHNLIAIAG
                     IGAAIGLSEVVADTGGTVSLFGTPAEEAVGGKIFMAQAGIFDGLDAAMGAHPGTIEAV
                     CPTVEGSGNALACREVIIKYHGEAAHAAADPFNGINALNALIEVFNGINALRQHVKTD
                     ARIHGIITDGGHAVNVVPDYAAGFFLIRAATLSYMDELVEKVRAIAEGAALITGARLE
                     WEFPSPACADMVTNYSLANVLDRHAREVGLEMPVAVAEPASGSTDWGNVSYVVPSVET
                     SFPIIDRVCTWHSMDVVNAADSDMGYENTIKVAKAMALAGLDVLRDAELRRSIRAEFD
                     DRLPVETREMVRSLV"
     gene            2181..>2387
                     /locus_tag="VFI12_04035"
     CDS             2181..>2387
                     /locus_tag="VFI12_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055606.1"
                     /translation="MVLSGSAGRSVARLGSETRVIGTVSPSAGHVPSATPESIFSASG
                     ARGVRRRTLKPLAPTGGVPVIAINA"
BASE COUNT          405 a          796 c          747 g          439 t
ORIGIN      
        1 gtcgtcggga tccaggaaga acgcgcaggc ttcggcgcct gggtcgcgcg tggatcgacc
       61 gtgacgcccg tctactacga cgcttccctg acgtcatgag cccggcactt cacggttcgg
      121 ggcagattcg cttttggctc tgcgtcggac tcgtgattgg catgctcggc ggcgtggctc
      181 tcgcctggcg atggaccgcc gccggcaccg gggcccgcgt cgcggtcctt ggcgccggca
      241 agtcggtgtc ggtgctgatc acgaatgacc atcggcgtgt cctgatcgcc tcaggcagca
      301 atggcaccgc cttttcgaac gcgctcggcg ccgcgctgcc gccattcgtc gacaacatcg
      361 atctggtgtt gatagatagt cgcgcgtcgg ccgacctgat cgaccgtgtc aaagcgctgg
      421 acgccaagcg gacgatgatg ctgcccgacc ctgaacggga cgatcccgcc gaaactatcc
      481 tgcgatcgtt ccaggtcgat ctcggcgagg gcgtaatcct gtccgtcaga atcgagcctg
      541 ccggcaagtg gtccgcggaa cttcggacca gcgccgggat cctctcgatc tcaccgggtg
      601 gcgtcgacct tgccccagcg ctggttcgga tcacgcttga tggaacgacg gctgtcgaac
      661 ccggcggtac tccgtcgata tggattgggc cagccgcgaa tgggctggtc cggacgtcgc
      721 atcaggcgac ggtcggcaca ggttcggtgc tgccgatcac aatggacgga tcagcatttc
      781 ggatcccgcg cgaattcttc ggtgcggtcg aatcagacca gcgaacgaac catttcccga
      841 gtctcgacgg gcaggcgatc gtcgaattcc gcccggattg accggcggag ctctgcatcc
      901 cgcaggacgt cgaggccggc cagcgccatc gccttcgcaa ccttgatcgt attctcgtag
      961 cccatgtccg agtcggccgc atttacgacg tccatcgaat gccatgtgca gacgcgatcg
     1021 atgatcggga aggatgtctc gaccgacggg acaacgtagc tgacattccc ccaatcggtt
     1081 gatccggatg ccggctcggc gacagccaca ggcatctcca gcccgacctc acgcgcatgg
     1141 cgatcgagca cattggcaag cgagtagttc gtgaccatat ctgcgcaggc gggactcggg
     1201 aactcccact cgagccgggc gccggtgatc agcgccgcgc cttcggcgat cgcgcggacc
     1261 ttctcgacca gctcgtccat atagctcagt gtggcggcgc ggatcaggaa gaagccggct
     1321 gcgtaatccg ggaccacgtt cacggcgtgc ccgccgtcgg tgatgatgcc gtggatccgc
     1381 gcgtcggttt tgacgtgctg gcgaagggca ttgatcccgt tgaagacctc tatcaaggcg
     1441 ttcagggcgt tgatgccatt gaacggatcg gccgccgcgt gcgccgcctc accatgatat
     1501 ttgatgatca cctcgcggca ggccaacgca tttccggagc cctcgaccgt cgggcaaacc
     1561 gcctcgatcg tgcccgggtg cgcgcccatc gcggcgtcca gcccgtcaaa gatgcccgcc
     1621 tgcgccatga agatcttgcc gccgactgct tcttccgccg gcgtaccgaa gagcgacaca
     1681 gtccctccgg tatcggccac gacctccgac aacccgatag ccgcgccgat accggcgatg
     1741 gcgatcaggt tatgtccaca cccgtgcccg agacccgcca gcgcgtcata ctccgagagg
     1801 tagccgacgt gcccgcccgc gcccgcgccg acagtcgctg agaacgccgt cggcaggttc
     1861 gcgacgccac gctggaccat gaagcctcga tcttcgacga atttggcgca cgccgcactc
     1921 gactgcacct cctgcatcgc gatctccggg tgtgaatgga tgaacttgct gagctcgatc
     1981 agcgcacccg cttcccggtc gatccggtcg atgattcgtt gctgaaggtc gtccacgttc
     2041 ccctctttcc aggtcatcct gaaatcaata gccgtccaca tcgcggacaa aagcgatcct
     2101 gctccgtgat ccgttcgccg catgataggc accgcatcgt gtcttcgcgc gccggtggat
     2161 agccgagcgg aagcgcagtc atggtcctgt cgggatcggc cggtcggtca gtcgcgcggc
     2221 tgggatcgga aacccgcgta atcgggaccg tctccccgag cgccggccac gtgccgagtg
     2281 caaccccgga gtcgatcttt tcagcttcgg gggcgagggg agtccgccgg cgcaccttga
     2341 agccgctcgc gcccacgggt ggcgtgccgg tgatcgcgat caatgcc
//
LOCUS       DASSGX010000230         2583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1569106, whole genome shotgun sequence.
ACCESSION   DASSGX010000230 DASSGX010000000
VERSION     DASSGX010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569106"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(371..1351)
                     /locus_tag="VFI12_04040"
     CDS             complement(371..1351)
                     /locus_tag="VFI12_04040"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7055607.1"
                     /translation="MLRFDRRKFVLSAAAAGAAFGLTKRVEVIPSALAQTADASPLNP
                     AGAKFHKFNVGDIEVTNIFDGAALRDHSPGFVNNASVDDVKAALRDAGMPDDKVPNSY
                     TVTVVKLGDKTIMFDSGNGAGRAPGVGLLAENMTAAGIDPGSLSAIVVTHFHPDHIFG
                     LMAGEDGQVYSDLEIIVPEAEYAFWSDPAAIEKLPEGRRGLARRVQATMPNWKNLSQV
                     AEEKDAVSGVRAVKTYGHTPGHTSYLVSSGSAQLMVLGDVTNIPPINLHNPGWHVAFD
                     QDAEVAEATRRSVFDRIVADQIVCAGYHWGMPGAGTIEKDGDGYVLKPVA"
     gene            complement(1519..2490)
                     /locus_tag="VFI12_04045"
     CDS             complement(1519..2490)
                     /locus_tag="VFI12_04045"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HET7055608.1"
                     /translation="MRRGKVIGAVALLAGAAVYLNNTSYLASPPADRPSLLAHRGLHQ
                     TYDRTGLTDETCTAARIDHPTHPHLENTIASIQAAFELGADVVELDVHPTTDGHFAVF
                     HDWTLDCRTDGHGVTREHSLAALKALDIGYGYTADGGKTYPFRGKAIGLMPSLSEVLT
                     AFPRQGLLINVKSNDGDEGRRLADALAQLPRDRLERIMVYGGDRPIRELRRRLPQLRT
                     MSRATLKSCILRYALTGWVGYVPDACRNTLLLVPTNVGPWLWGWPHAFLGRLARHDTL
                     VFAVGPYHGGDFSNGINTPDELALLPRNYAGGIWTDRIDVLGKLVAK"
BASE COUNT          445 a          835 c          839 g          464 t
ORIGIN      
        1 gaactatggc gcaaggcccg ccgcctgcca gaatgccgcg accaccggct gtgctggagc
       61 gcgctcaagc ggtgcagaac gaggagcctg tcctcaaccg cgcagcttgt gtttcggtgc
      121 acgcgacgtc tccggttcac gctgaccggc agaactgtcc tcggcaatcg ttgacgccga
      181 gtgaacggtc gttaccaagc aagttggatg ccaattatgg cgatggcggg aatggcacgc
      241 ttcgcggcag ccacaagggc tgcttcgaag cgtgccgccg atgtgaaaag accaggcgct
      301 gacctgtggt caatcgcctg gcccttgctt cattttgtcg aggacgggct acgagccccg
      361 accgtctaac ttacgccacc ggcttcagga cgtagccgtc gccgtccttc tcgatggtgc
      421 cggcgcccgg catgccccaa tggtagccgg cgcagacgat ctggtcggca acgatgcggt
      481 cgaagacgct gcgccgcgtg gcctcggcca cctccgcgtc ctggtcgaac gcgacatgcc
      541 atcccggatt gtgcagattg atcggcggga tgttggtcac gtcgcccagc accatgagtt
      601 gcgcgctgcc cgaggacacg agatagctcg tgtgcccggg cgtgtggcca taggtcttca
      661 cagcccgcac gccggacacc gcgtccttct cctccgcgac ctggctgagg ttcttccagt
      721 tcggcatggt ggcctgcacg cgccgcgcaa gcccgcgccg gccctccgga agcttctcga
      781 tggcggccgg atcggaccag aaagcgtact cggcctccgg cacgatgatc tcgagatccg
      841 agtagacttg gccatcctcg ccggccatca gcccgaagat gtggtcgggg tggaaatgtg
      901 taaccacgat cgcgctgagg ctgccggggt cgatgccggc ggccgtcatg ttctcagcga
      961 gcagccccac gcccggcgcg cgtcctgcac cgttgcccga gtcgaacatg atggtcttgt
     1021 cgccgagctt gaccacggtg accgtgtagg agttgggcac cttgtcgtcg ggcatgcccg
     1081 cgtccctcag cgccgccttg acatcgtcga cgctggcatt gttcacgaat ccggggctgt
     1141 gatcgcgcag cgcggctccg tcgaagatgt tggtgacctc gatgtcaccg acgttgaact
     1201 tatggaactt tgccccagcg ggattgagtg gcgaggcgtc ggccgtctgg gcgagcgccg
     1261 aggggatgac ctcgacacgc ttcgtcagcc cgaaagcggc gccggccgcc gcggctgaca
     1321 gcacgaattt gcgacgatcg aatcgcagca cgttgacctc ccattcgttc gttggcgtcc
     1381 acggacgtgc cgggaccgat ggcacacgat cctagttagg cttggccggc aggcgaaacg
     1441 cattgcgccc cgcatcacgg catattgaag ccccctcgtg tagaggcggc ggttcacgtg
     1501 aaacgctcag cgacgagatt acttcgcaac cagcttcccg aggacgtcga tgcggtcggt
     1561 ccagatcccg ccggcatagt tcctcggcag caatgccagc tcgtcgggcg tattgatgcc
     1621 gttagagaag tccccgccat ggtacggacc gaccgcaaag acgagcgtgt cgtgcctcgc
     1681 cagtcttccc aggaaggcgt gcggccagcc ccaaagccac gggcccacat tcgtggggac
     1741 gagcagcaat gtgttgcgac aggcgtccgg aacatatccg acccagccgg tgagggcgta
     1801 tctcagaatg cagctcttga gggttgcgcg ggacatggtc cgcagttgag gcaggcgccg
     1861 tcgcagctcc cggatcggcc gatcaccgcc gtagaccatg atgcgttcaa gccgatcgcg
     1921 cggcagttgc gccagtgcgt cggcgagccg ccgcccctca tccccgtcgt tgctcttcac
     1981 gttgatcaac agcccctggc ggggaaacgc cgtgagcacc tcgctcaagc tcggcatcag
     2041 accgattgcc ttccctcgga atggataggt cttgccgccg tcagccgtat agccgtagcc
     2101 gatatcgagc gctttcagcg cagcaaggct gtgctcacga gtgaccccgt ggccgtccgt
     2161 gcggcaatcg agcgtccagt cgtggaaaac tgcgaagtgg ccgtcggtcg tcgggtgcac
     2221 gtcgagctcc acgacgtcgg cccccagctc gaatgcggcc tggatcgagg cgatggtgtt
     2281 ctcgagatga gggtgcgttg gatgatctat ccgagcggcc gtgcaggtct catccgtgag
     2341 cccggtgcga tcgtaagtct ggtgcagccc gcgatgcgca agaagggaag gccgatcggc
     2401 tggaggggac gcgagatagc tcgtgttgtt gagatagacc gccgcccctg ccaacagcgc
     2461 cacagcgccg atcaccttgc ctcgtcgcat gctccgctct cctgccgcgc ccccccctga
     2521 gaggccgcag caaagagacg cgactttgtg gccggcagcg ggcgagcttg ggcgagagtc
     2581 agc
//
LOCUS       DASSGX010000231         2532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_43750, whole genome shotgun sequence.
ACCESSION   DASSGX010000231 DASSGX010000000
VERSION     DASSGX010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43750"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..942
                     /locus_tag="VFI12_04050"
     CDS             100..942
                     /locus_tag="VFI12_04050"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HET7055609.1"
                     /translation="MHGIRRLFVILVAPMLMLTWSAAALATTTGSPVPSDTEEPTQAE
                     PSVFIRQDPALGTILTDPTGRTLYHLTKDTTKGESTCYDTCAQNWPPFTATEPLSLPA
                     ETNGELSTIKRTDGTTQVAYNGIPLYYFAKDTAPGQTNGQEVGDVWYVVTPGQQFGET
                     YEPAGTPVANLGTPAAAGEVTVMLTEMSISASATTFKVGQTYTFTATNQGAIEHELVF
                     EKVGDNDVPLVSNGKEVELESIAPGSSKSLDFAFTEAGTYQLACHVPGHYPAGMVLTI
                     SVVN"
     gene            complement(939..1700)
                     /locus_tag="VFI12_04055"
     CDS             complement(939..1700)
                     /locus_tag="VFI12_04055"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7055610.1"
                     /translation="MTNDLSDALDMIIRGFARLERGVHPVDEDAVGPVRFLRWPERGR
                     RGQEFSEEFFALHPDPAELLAVVREVGPGPDHLIAVVSTEPEPDQAPYLAAGYEFGSR
                     EPLMLTALTNANSTPDPALDVHRVTTMAEVARILAAQQSVGYPERLFTEALLADPHVS
                     IRAVFVDGQFVAIGKLAVVESGGYITDVMTMPGHRRQGYGAGVVRQLHADARAAGAER
                     SALTSTAMARSLYEQRGYRQIGFVTLYATPERVTG"
     gene            1840..>2532
                     /locus_tag="VFI12_04060"
     CDS             1840..>2532
                     /locus_tag="VFI12_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055611.1"
                     /translation="MKPFVFDPKSSDPQSVIGAVIPADVRVEGKRRFRKGHRFTAEDL
                     PDLAKVEHPIHVIQIEPDEVHEDEAAIALAEAVSGQGVERRKPVQSRVNMIADRKGLL
                     RVDRDRVIALNRMPGISVFTLLDGLAVVPGKNIAGAKISPVAVKRSVLDEALALAAEP
                     GPVVEVKPFTPKQVSVVTTEGLGEKVWDRFRSAVEQKIGWYGSTVTGFTDLIDDPDYV
                     AGAITDAIDAGAD"
BASE COUNT          462 a          872 c          765 g          433 t
ORIGIN      
        1 ctccgtgcat ccatatgtcc gttcgcggcg tagggactga cggcgacccc tccccgttca
       61 tttgacgctc tacccgcacg tcgccacgga ggaaagtcca tgcacggcat tcgcaggttg
      121 ttcgtcattc ttgtcgcgcc gatgctgatg ctcacctgga gcgcggctgc gctcgccact
      181 acgaccggct ctccagtacc aagcgacacg gaagagccga cgcaagcgga gccgagcgtc
      241 tttattcggc aggacccagc cctcggcacg atccttactg acccgacggg aaggacgctc
      301 tatcacctca cgaaggacac gaccaagggc gaaagcacct gctacgacac ctgcgcccag
      361 aattggccac ccttcaccgc gacagaaccc ctctcgctac cggccgagac taacggtgag
      421 ttgagcacga tcaagcgcac cgacggcacg acccaggtcg cctataacgg catcccgctc
      481 tactacttcg ccaaggacac tgctcccggc cagaccaacg ggcaagaggt gggcgatgtc
      541 tggtacgtgg tcacgccggg ccagcagttc ggtgagacat acgagcctgc cggcacgccc
      601 gtcgcgaatc tgggtacgcc ggccgccgcg ggtgaggtca cggtcatgct caccgagatg
      661 tcgatctcag cctcggccac gacattcaag gtcggccaga cgtacacctt taccgcgacg
      721 aaccagggcg caattgagca tgagcttgtc ttcgagaagg ttggagacaa cgacgttccg
      781 ctcgtgagca acggcaagga agtcgagctc gaaagcattg cgcccggttc atccaaatcg
      841 ctcgatttcg ccttcaccga ggccggcacc tatcaactgg cctgccacgt tccaggccac
      901 tatccggccg gcatggtgct gacgatctcc gtcgtcaact aaccggtgac ccgctcgggc
      961 gtcgcgtaga gcgtgacgaa cccgatctgt cggtacccgc gctgttcgta gagcgatcga
     1021 gccatggccg tcgaggtcag agccgatcgc tccgcccccg ccgcccgggc atcggcatgc
     1081 agctggcgca cgacccccgc gccgtatccc tgccgccggt gcccgggcat cgtcatcaca
     1141 tcggtgatgt acccgccact ctcgaccacg gccagcttgc cgatcgccac gaactggccg
     1201 tcgacgaaaa ccgcccggat gctgacgtgc ggatccgcga gcagcgcctc cgtgaacaac
     1261 cgttctggat agccgaccga ctgctgcgcc gccagaatcc gcgcgacctc agccatcgtc
     1321 gtgacccgat ggacgtcgag cgcgggatcg ggcgtgctgt tggcgttggt cagcgcggtc
     1381 agcatcagcg gctcccgcga tccgaactcg tacccggcgg cgaggtacgg cgcctgatca
     1441 ggctctggtt cggtggagac gacggcgatc aggtgatcgg ggcccggtcc aacttcccgg
     1501 acgacggcga gcagctccgc cggatccgga tggagcgcga agaactcttc gctgaactcc
     1561 tgcccccgcc gcccccgctc cggccagcgc agaaaccgca ccggcccaac cgcatcctca
     1621 tcaaccgggt gcaccccccg ctccaaccgc gcaaacccgc ggatgatcat gtcgagcgcg
     1681 tctgagaggt cattggtcat tggtgtctcg tctttcgtct tgggttttgg gttttgggtt
     1741 ttgggttttc ggagagagtc tagccattgc ttctcgactc ctcgacttct cgattcctcg
     1801 actcaggcgc ctccgcgcct tcgcgcctac actactcata tgaaaccctt cgtcttcgac
     1861 ccgaaatcgt ccgatccgca atcggttatc ggcgccgtca ttccggccga tgtccgcgtc
     1921 gagggcaaac gacgcttccg caaggggcat cgcttcaccg ccgaggacct gccggatctc
     1981 gcaaaggtcg agcacccgat ccacgtcatc cagatcgaac cggacgaggt ccacgaggac
     2041 gaggccgcga tcgcgctggc cgaagccgtc tcggggcagg gtgtcgagcg gcgcaaaccg
     2101 gtccagagcc gcgtcaacat gatcgccgac cgcaagggac tgctgcgggt cgaccgggat
     2161 cgggtgatcg cgctcaaccg gatgccaggc atctccgtct tcaccctgct cgatggcctc
     2221 gccgtcgtcc ccggcaagaa catcgccggc gcgaagattt ccccggtcgc cgtcaagcgg
     2281 agcgtgctgg acgaggcgct ggcgctcgcg gccgagccgg gaccggtggt ggaggtcaag
     2341 cccttcacgc ccaagcaggt ctctgtcgtg acgaccgaag ggctcggcga gaaggtctgg
     2401 gatcgtttcc gctcggcagt cgagcagaag atcggctggt atggcagcac cgtgaccgga
     2461 tttaccgacc tgatcgacga cccggactat gtcgccggcg caatcaccga tgcgatcgat
     2521 gccggcgcgg ac
//
LOCUS       DASSGX010000232         3447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_587930, whole genome shotgun sequence.
ACCESSION   DASSGX010000232 DASSGX010000000
VERSION     DASSGX010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587930"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1570
                     /locus_tag="VFI12_04065"
     CDS             <1..1570
                     /locus_tag="VFI12_04065"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HET7055612.1"
                     /translation="LLLSSVEALAAFDVAFAKARMAMQMSAIRPALAAESDRAIRLTQ
                     ARHPLLDPQTVVPIDIEVGGAFRLLVITGPNTGGKTVALKTAGLLGLMAQTGLFIPAA
                     EGSQLPVFDAVFVDIGDEQSIQQSLSTFSSHMTNVVRILKTVGPRDLVLLDELGAGTD
                     PQEGSALARALLDEFLKTGALVIGTTHYTEVKAYASTTEGVENGSVEFDLKTLSPTYR
                     LAIGMPGQSNALAIAKRLGLPGSIVDSARALLDPDAIRTENYLSEIRARRTDAERTLG
                     KAREIEREAKQLRRLAREALREAEDQRRTAREEAVSQAEIELAEIRDLSRRLERDHRT
                     TVAARDEIEERRRSIEAAQQTVREFRREHVAPVRLAESTEIKPGDRVRVVAFDEEGEV
                     LAIEDGSADVQMGIIKIRQPLDGLRRLGRVQVTAASERRVSRPAAGPLVPIEIDLRGY
                     RAHEIEGELDPYLEQAYRSGMPFVRIIHGKGTGALRQVVRDLLSRSPAVARFETAGPN
                     EGGDGATVAFFHDR"
     gene            complement(1558..>3447)
                     /gene="mfd"
                     /locus_tag="VFI12_04070"
     CDS             complement(1558..>3447)
                     /gene="mfd"
                     /locus_tag="VFI12_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HET7055613.1"
                     /translation="LLEYAKGDKLYVPVDQSDRVSRYSSGGLEPRLTRLGTGEWQRAK
                     SRVRRAVREMAFELIQLYAARESGEGIQYPPNTTWDEELAESFAFVETADQQSAIIDV
                     TGDLETDRPMDRLVCGDVGFGKTEVALRAAFKVINAGKQVAVLVPTTVLALQHYATFR
                     QRLAAFPARVEMLSRLKSRSEQGEIVEGLAEGSVDIVIGTHRLVQNDIRFQDLGLVVI
                     DEEQRFGVRHKEFLKRLRTQVDVLTMSATPIPRTLHMSLAGIRDISIINTAPQERLPI
                     RTFVTDYSESLVREVILREIDRGGQVYFVHNRVHSIDHLAARLRELVPEARFGIGHGQ
                     MEEDVLEDVILGFVRHEFDVLICTTIIESGVDIANVNTIVIDDADRFGLTQLYQLRGR
                     VGRGSHRAYAYLLHKPHKALSEVAQARLDAIQEATELGAGMQVALRDMEIRGAGNVLG
                     AEQSGNIADVGYDLYIRLLSQAVEEIRQGRPFAEPTPLTLDLPLMALIPASYISDVEL
                     RLNTYRRIASAKTLADVDDVEAELRDRFGEPPEEVEHLIALIRVRIRCAALGMQSVVE
                     REREIVLRPVETTRLKGLGLERVIGKALRTTPNSVRIRMPDLEIPWEQALEAVIGAVE
                     MTQRS"
BASE COUNT          582 a         1128 c         1146 g          591 t
ORIGIN      
        1 gttgctgttg agctcggtcg aggcgctcgc cgcatttgat gtcgccttcg ccaaagcgcg
       61 catggcgatg cagatgtcgg ccatccgccc ggcattggcc gcggagagcg atcgggcgat
      121 ccggctgacg caggcgcgtc accccttgct ggacccgcag acggtcgtcc cgatcgacat
      181 cgaggtcggc ggcgcgttcc gcctcctggt catcaccggt cccaataccg gcggcaagac
      241 ggtcgcgctc aagacggcgg gcctgcttgg cctgatggcg caaaccgggc tcttcatacc
      301 ggccgccgag gggagccagc tcccggtctt cgacgcggtg ttcgttgaca tcggcgatga
      361 gcaatcgatc caacagagcc tgtcgacctt ctcgtcgcac atgacgaatg tggttcggat
      421 cctgaagacc gtcgggccgc gggacctggt tctgctcgac gagctcgggg ccgggaccga
      481 tcctcaggaa ggatcggcgc ttgcccgcgc gttgctggac gagttcctga agaccggcgc
      541 tctggtcatc ggcaccacgc actacaccga agtcaaggca tacgcatcaa ccaccgaagg
      601 tgtcgaaaac ggcagtgtcg agttcgacct caagacgctg tcgccaacgt atcgactggc
      661 gatcgggatg ccgggacaat cgaacgcgct cgcgatcgcc aagcgactgg gccttccggg
      721 gtcgatcgtg gattcggccc gggcattgct cgatccagac gcgatccgca ccgagaacta
      781 cctgtcggaa atccgggcca ggcgtaccga cgcggagcga accctcggca aggcgcgcga
      841 gatcgaacgc gaggccaagc agctccggcg tctggcgcgc gaggcattgc gcgaggccga
      901 ggatcagcgg cgtaccgcgc gcgaagaagc cgtgagccag gcagagatcg aacttgccga
      961 aatccgggac ctgtcgaggc ggctcgagcg cgatcaccgg acgaccgtcg cggcccggga
     1021 cgagatcgag gagcgtcgcc gttcgatcga agccgcccag caaaccgtgc gcgagttcag
     1081 gcgcgagcac gtcgcgccgg ttcgcctcgc ggaatcgacc gagatcaagc cgggcgatcg
     1141 ggtacgggtc gtggcgtttg acgaagaggg cgaagtcctc gcgatcgaag acggctccgc
     1201 ggacgtgcag atgggcataa tcaagatccg gcagccgctc gacggcctcc gccgcctcgg
     1261 tcgggtccag gtgacggccg cgagcgagcg ccgggtctca aggccggcgg ccggccccct
     1321 tgtcccgatc gagatcgacc tgcggggata ccgcgcccac gagatcgagg gcgagctcga
     1381 cccgtacctc gagcaggcgt accggtcagg catgccattc gtgcggatca tccacgggaa
     1441 gggaaccggc gcgcttcggc aggtggtcag ggatctcctg tcccggtcac cggccgtcgc
     1501 ccggttcgag accgccgggc cgaacgaggg cggcgacggc gcgacggtgg ccttctttca
     1561 cgatcgctga gtcatctcta ccgcaccgat cacggcctca agcgcctgct cccacgggat
     1621 ttccaggtcc ggcatgcgga tccgcactga attgggtgtc gtccttagcg ctttgccgat
     1681 cacccgctcg aggccgagcc ccttgaggcg cgtagtctcg accggccgga gcacgatctc
     1741 gcgctcgcgt tcgacgaccg actgcatccc gagcgccgca caccgaatcc ggacccggat
     1801 cagcgcgatc aggtgctcga cctcttccgg tggctcgccg aagcggtccc tcagctcggc
     1861 ttcgacgtca tctacgtcag ccagcgtctt ggccgaggcg attcggcggt aggtattgag
     1921 ccggagttcg acatccgaga tgtaacttgc cggaatgagg gccatgagcg gtagatcgag
     1981 cgtcagcggg gtcggttcgg cgaacggccg gccctgacga atctcctcaa ccgcctggct
     2041 cagcagacgg atgtagaggt cgtagccgac atcggcgatg ttgccgctct gctccgctcc
     2101 gagcacgttg ccggctcccc ggatctccat gtcccggagc gccacctgca tcccggcgcc
     2161 gagctccgtc gcctcctgga tcgcatcgag ccgtgcctgg gcgacctcgc tgagcgcctt
     2221 gtgcggtttg tgcagcaggt aggcgtaggc gcgatgggag ccgcggccga cccgtccccg
     2281 gagttggtaa agctgggtca ggccgaagcg gtcggcgtca tcgataacga tggtgttgac
     2341 attcgcgata tcgacacccg attcgatgat cgtcgtgcag atcaggacat cgaactcatg
     2401 acgaacgaag ccgaggatca cgtcctcgag gacgtcctcc tccatctggc catgaccgat
     2461 gccgaagcgc gcttcgggta ccagttcgcg gagccgggcg gcgaggtggt cgatcgaatg
     2521 cacgcggttg tggacgaaat agacctgtcc cccgcgatcg atctcccgca ggatgacctc
     2581 gcgcaccagg gattcgctgt agtcggtcac gaaggtgcgg atcggtagcc gttcctgcgg
     2641 cgcggtgttg ataatgctga tgtcccgtat gccggcgagg gacatgtgga gcgtgcgcgg
     2701 gataggggtc gcgctcatgg tgaggacatc gacctgggtg cgcagccgct tcaggaactc
     2761 cttgtggcgg acgccgaatc gctgctcctc gtcgatcacc accaatccga gatcctggaa
     2821 gcggatgtcg ttctggacca gccggtgcgt gccgatcacg atgtcgacgc tgccttcagc
     2881 gagcccctcg acgatctccc cctgctcact ccgtgatttg aggcgggaca gcatctccac
     2941 gcgcgccggg aaggccgcga gccgttgccg gaatgtcgcg taatgttgca gggcgagcac
     3001 ggtcgttggc acgagcacgg ccacctgctt gcccgcgttg atcactttga aggcggccct
     3061 gagcgcgacc tctgtcttgc cgaagccgac gtcgccgcag accagccggt ccatcggtcg
     3121 atccgtttcc aggtccccgg tcacatcgat gatggcggat tgctggtcgg ccgtctcgac
     3181 gaacgcgaac gattccgcca actcttcgtc ccaggtcgtg ttgggcggat actgaatgcc
     3241 ctcgccgctt tcccgggccg cgtagagctg gataagctcg aaggccattt cgcggacagc
     3301 tcgccggacg cggctcttgg cccgctgcca ctctccggtg ccgagccggg tcagccgagg
     3361 ctcgagtccg ccgctcgaat accggctgac ccggtcactc tggtccaccg gcacgtagag
     3421 cttgtcgccc ttcgcgtact cgagcag
//
LOCUS       DASSGX010000233         2377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1241040, whole genome shotgun sequence.
ACCESSION   DASSGX010000233 DASSGX010000000
VERSION     DASSGX010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241040"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            237..2033
                     /locus_tag="VFI12_04075"
     CDS             237..2033
                     /locus_tag="VFI12_04075"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7055614.1"
                     /translation="MISEFSVAGSYRYDRSGPVRWIVSHIWRHKYFAITAALAMIAHN
                     GLNSLVQWLIGRAFDRLLEPTPDRGALVSITIALIVIVACRSVLDLGASFLAQVTAQR
                     LERDGREELYQSLLGKSQTFHNKQRVGDLMARANNDVRQLTMMIQPGAALIIDSFSAL
                     FVPIIFIAFLKWQLLLSPLLFTVVFIVLLRRYMRKMEPVAGRQRMEFGQMNSVLTESV
                     TGIEVVKSTAQEAQETAKFLTRARSYRDAAVRQGRLEAGYLPTLFVGFAIAGAAVHGL
                     ILIDRGQLTVGQLVTYLGLMGLLRFPAYMSIFSFSLIQLGRVSDRRILDLITERTDLD
                     QNTGGHTGRIGGKIEFADVSFTYGGEPVLAHVSFTVQPGETVALVGETGSGKSTLTKL
                     VNRIYDATGGAVRIDDVDVRDWQLDTLRSQISTIEQDIVLFSRPVAENIAFSLGQDAP
                     IEAIRQAARDAQADGFISEMPDGYETVIGERGMTLSGGQRQRIAIARALLTDPRILIL
                     DDSTSAIDSATEDQIQRAIKAISADRTTLLITHRLSQIRWADKVLVMRHGHVVDFGTH
                     DELLARCDLYQRIFARYDEAPERAAVTVGEAS"
     gene            2033..>2377
                     /locus_tag="VFI12_04080"
     CDS             2033..>2377
                     /locus_tag="VFI12_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055615.1"
                     /translation="MAFIMNGLDAENYSRTYTDRQLIDRIKTYFRPEAAKIGLVTVMV
                     VLKSVFDAALPILIATGLDRVVGPDGVDNRVWWLVVGIIVFGALSWGVNFVQQFKSAE
                     IVGNIILRLRTDA"
BASE COUNT          377 a          847 c          758 g          395 t
ORIGIN      
        1 gatgatcgat gcggcccacg gggggaccgc gctccgccgg tgcatgcaaa cgcgccggtt
       61 ggggaggacg ggcagatgcc atctgcccct acatcggggg ccggtgcctg cgaacgggcc
      121 ggtcaggacg gcgggtttgc ccccgcaaac ccctacaatg ctccgcacct ccgcgcctgc
      181 tcgcctcgta cgcatgtgct agcatgggca gccggagcat ttgcggaggc attcccttga
      241 tttccgagtt cagcgttgcg gggtcctacc ggtatgaccg gtcggggccg gtccggtgga
      301 tcgtgtcgca catctggcgg cacaagtact ttgcgatcac cgcggccctg gccatgatcg
      361 cccacaacgg gttgaacagc ctcgtccagt ggctgatcgg ccgcgccttc gaccgcctgc
      421 tcgaacccac gccggaccgc ggcgccctcg tctcgatcac catcgccctg atcgtcatcg
      481 tcgcctgccg ctccgtgctc gatctcggcg cgtccttcct cgcccaggtc accgcccagc
      541 ggctggagcg cgacggccgg gaggagctct accagtcgct gctcggcaag agccagacct
      601 tccacaacaa gcagcgcgtc ggcgacctga tggcccgggc caacaacgat gtccgccagc
      661 tgacgatgat gatccagccg ggcgcggccc tgatcatcga ctccttctca gccctcttcg
      721 tgccgatcat cttcatcgcc ttcctgaagt ggcagctcct gctctcgccg ctcctcttca
      781 cggtcgtctt catcgtcctg ctccgccgct acatgcggaa gatggagccg gtcgcggggc
      841 ggcagcgaat ggagttcggc cagatgaact cggtcctgac cgagtcggtg acgggcatcg
      901 aggtggtcaa atcgaccgcg caggaggccc aggagacggc caagttcctg acccgggcgc
      961 gctcctaccg ggatgccgcc gtgcgccagg gacggctcga ggccgggtat ctgccgactc
     1021 tcttcgtcgg cttcgcgatc gcgggagcgg ccgtccacgg gctgatcctg attgatcgcg
     1081 gccagctcac ggtcggccag ctcgtcacct acctcggcct gatggggctg ctccgcttcc
     1141 cggcctacat gtcgatcttc tccttctcgc tgatccagct gggccgggtc agcgaccgcc
     1201 gcatcctcga cctgatcact gagcggaccg acctcgacca gaacaccggc ggccacactg
     1261 ggcggatcgg cggcaagatc gagttcgcgg acgtctcctt cacctacggc ggcgagccgg
     1321 tgctggcgca tgtctcgttc acagtccagc cgggcgagac ggtcgcgctc gtcggcgaga
     1381 ccggctccgg caagagcacc ctgaccaagc tggtcaaccg catctacgac gcgaccggcg
     1441 gcgccgtccg gatcgacgac gtcgacgtgc gcgactggca gctcgacacg ctccgctcgc
     1501 agatctcgac catcgagcag gacatcgtcc tcttctcgcg gcccgtcgcg gagaacatcg
     1561 ccttcagtct cggccaggac gcgccgatcg aggcgatccg ccaggccgcg cgcgatgccc
     1621 aggccgacgg cttcatcagc gagatgcccg acggctacga gacggtcatc ggcgagcgcg
     1681 gcatgacgct ctccggcggg cagcggcaac ggatcgcgat cgccagggcg ctgctgaccg
     1741 acccgcgcat cctgatcctc gacgattcga ccagcgcgat cgactccgcc accgaggacc
     1801 agatccagcg cgcgatcaag gcgatcagcg cggaccggac gacgctgctg atcacccacc
     1861 gcctcagcca gatccgctgg gccgacaagg tgctggtcat gcgccacggc cacgtcgtcg
     1921 acttcggcac gcacgacgag ctgctggccc gctgcgacct ctaccagcgc atcttcgcgc
     1981 ggtacgacga ggcgccggag cgggcggcgg tcacggtcgg ggaggcgagc tgatggcctt
     2041 catcatgaac ggcctcgacg ccgagaacta cagccgcacc tacaccgacc gccagctgat
     2101 cgaccggatc aagacctatt tccgccccga agcggcgaag atcggcctcg tcacggtcat
     2161 ggtcgtgctc aagtcggtct tcgatgccgc cttgccgatc ctgatcgcga ccgggctgga
     2221 ccgcgtcgtc ggtccggacg gcgtcgacaa ccgggtctgg tggctggtgg tcgggatcat
     2281 cgtcttcggc gcgttgtcgt ggggcgtcaa cttcgtccag cagttcaaat cggccgaaat
     2341 cgtcggcaac atcatcctgc ggctgcggac cgacgcg
//
LOCUS       DASSGX010000234         2437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_261794, whole genome shotgun sequence.
ACCESSION   DASSGX010000234 DASSGX010000000
VERSION     DASSGX010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261794"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="VFI12_04085"
     CDS             complement(<1..631)
                     /locus_tag="VFI12_04085"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET7055616.1"
                     /translation="MSFLSTDSPIWQDAALIQRARDVGAQFADRVIETARVIAMVAAP
                     TNDEHDRAVLVADLFQTHGARDVTTDDIHDVVGRMAGKTHETAVLIAAHTDTVFSRET
                     VLKIHQDDERGYGPGLGDNSLGVASVVFLPEMLAALDAVPATDLLLTGNVGEEGLGNL
                     RGITRVLTDHEDTGAVIAVEGQNLGRITHIAVGSVRLRITVTGPGGHSWG"
     gene            823..>2437
                     /gene="sdhA"
                     /locus_tag="VFI12_04090"
     CDS             823..>2437
                     /gene="sdhA"
                     /locus_tag="VFI12_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HET7055617.1"
                     /translation="MGYHRYDAVIVGAGGAGLMAAIQMAGKANVAVVSKLYPTRSHTG
                     AAQGGISAALGNTEEDHWEWHMFDTVKGGDYLVDQDAAEVLAREAIDAVLELEHMGLP
                     FNRTPEGKIDQRRFGGHTKNFGEAAVRRACYAADRTGHMIIQTLYQQGIKHDVHFFDE
                     HYMTDLIFSDDGRCCGVVTRDLANGEINVLHAKCVLIATGGFGRVFKITSNAHAGTGD
                     GVAIAYRRGVPLMDMEFYQFHPTGLYKLGILLSEAARGEGGILRNSEGEAFAARYAPT
                     LADLAPRDMVSRFIYQEVKEGRGIDGKDYVLLDLTHLPESVIDEKLPDITDFARTYLG
                     VEPKKEGVPIQPTAHYAMGGLPTNVEGQVLMNEEGRVIDGLYSAGESACVSVHGANRL
                     GTNSLVDLVVFGRRAGRHMLEYIKAADLPMLPPEPELMTRAELSEITGRTKGESIAAI
                     RDRMQVVMMDKVSVVRSREGLTEAQQELTELRDAYNHAAIQDKGQIFNTDLMEALELG
                     YMLDCADTIVGGALLREESRGAHYRTDFEA"
BASE COUNT          453 a          776 c          791 g          417 t
ORIGIN      
        1 cgccccagct atgcccgccc ggaccggtca cggtgattcg caagcggacg ctgcctaccg
       61 cgatgtgcgt gatccgcccg agattctggc cctcgacggc aatgacggcg ccggtgtcct
      121 catggtcggt cagcacccgg gtaatgccgc gcagatttcc gagaccctct tcgccgacgt
      181 tgcccgtcag caggagatca gtcgctggca cggcatcgag cgcagccagc atctcgggca
      241 ggaatacgac cgatgcgaca ccgagcgagt tgtcgccgag acctgggccg tacccgcgct
      301 cgtcatcctg gtggattttg agcacggtct cgcgcgagaa gacggtgtcg gtatgggcgg
      361 cgatcagaac cgccgtctcg tgtgtctttc cggccatgcg gccgaccaca tcgtgaatgt
      421 cgtcggtggt gacgtcgcgt gccccgtggg tctggaacag gtccgcgaca aggacggccc
      481 gatcatgctc atcgtttgtc ggcgcggcga ccatggcgat caccctggcc gtctcgatta
      541 cccggtccgc gaactgggcg ccgacgtcgc gcgcgcgctg aatcagcgcc gcatcttgcc
      601 agatcggcga atcggttgaa agaaatgaca tgctggatcc gttctcgggc gcgcatcgtc
      661 ggcgcgacgt ggtcggggcg aagcggtggc tcgacggcga aattataagc cgagccacgg
      721 tgccgtgttc gaacggggtt ccttgacgat tatcgggcag gtagatacgt taccgagagc
      781 gtttgtgacc agtcacgttt ccatcttcga ggagtcagcg ccatgggata tcaccggtac
      841 gatgcagtga ttgtcggcgc cggtggagct gggctcatgg cggcgatcca gatggccggc
      901 aaggccaacg tcgccgtcgt cagcaagctc tatcccaccc ggtcgcatac cggcgcggcc
      961 cagggcggca tcagcgccgc gctcggcaac accgaggaag accattggga atggcacatg
     1021 ttcgacaccg tcaagggtgg cgactacctg gtggaccagg acgcggccga ggtgcttgcc
     1081 cgcgaggcga tcgatgcggt cctcgaactt gaacacatgg ggcttccgtt caaccgcacg
     1141 cccgaaggca agatcgatca acgccggttc ggcggccata cgaagaactt cggggaagcc
     1201 gcggtgcgcc gggcctgcta cgccgccgac cgcaccggcc acatgatcat ccaaaccctc
     1261 taccaacagg gcatcaaaca cgatgtccat ttcttcgacg agcactacat gaccgacctg
     1321 atcttctcgg atgacggccg gtgctgcggc gtcgtgaccc gagacctggc aaacggcgag
     1381 atcaatgtcc tccatgccaa gtgcgtcctg atcgcgaccg gcgggtttgg ccgggtcttc
     1441 aagatcacgt cgaatgcgca cgccggcacc ggcgatggcg tcgcgatcgc gtaccggcgc
     1501 ggagtgccgc tgatggacat ggagttctac cagttccatc cgaccggtct ctacaagctt
     1561 ggcatcctgc tctcggaagc agcgcgcgga gaaggcggca tcctccgcaa cagtgagggc
     1621 gaggccttcg ccgcccggta cgcgccgacg ctggccgacc tggccccgcg cgacatggtt
     1681 tcccgcttca tttaccagga ggtcaaggaa ggccgcggga tcgacggcaa ggattacgtc
     1741 ctgcttgacc tgacgcacct tcctgagtcg gttatcgacg agaagttgcc ggatatcacc
     1801 gatttcgccc gcacctatct cggcgtcgaa ccaaagaagg agggcgtgcc gatccagccg
     1861 accgcgcatt acgcgatggg cgggctcccg acgaacgtcg agggccaggt cctgatgaat
     1921 gaggagggcc gcgtcatcga cggcctctac tcggccggcg aatcggcgtg cgtctcggtc
     1981 catggagcaa accggctggg tacgaactcg ctggtcgacc tggtcgtttt cggtcgccgg
     2041 gccggacgcc acatgctcga gtacatcaag gcagccgatc tgccaatgct gccacccgaa
     2101 ccggagctga tgacccgggc ggagctctcg gagatcaccg gccggaccaa gggcgaatcg
     2161 atcgccgcaa tacgtgaccg gatgcaggtc gtgatgatgg acaaggtctc ggtcgtccgg
     2221 tctcgcgagg gcctgacgga agcccagcag gagctgaccg agcttcgcga tgcctacaac
     2281 cacgccgcga tccaggacaa gggccagatc ttcaacaccg acctgatgga ggcgctcgag
     2341 ctcggctaca tgcttgattg cgcagacacg atcgtgggcg gcgcgctcct ccgcgaagag
     2401 agccgcggcg cgcattatcg caccgacttc gaggcgc
//
LOCUS       DASSGX010000235         3019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1133899, whole genome shotgun sequence.
ACCESSION   DASSGX010000235 DASSGX010000000
VERSION     DASSGX010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133899"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VFI12_04095"
     CDS             complement(<1..441)
                     /locus_tag="VFI12_04095"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HET7055618.1"
                     /translation="MPGNLTGLLPRLSDYPNIRANWKADLVAGLTVGVVALPLALAFG
                     ITTGLGAASGLVTAVVAGLVAAVFGGSHVQVSGPTGAMTVVLVPIVAHYGTDAVFAVS
                     IMAGVMIMLGGVLRLGRYLEYMPWPVFEGFTVGIAIIIFLQQVPN"
     gene            662..1510
                     /locus_tag="VFI12_04100"
     CDS             662..1510
                     /locus_tag="VFI12_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HET7055619.1"
                     /translation="MSINPTDLSQPVIISAALTGSWPTKADNPAVPITEEEIAEAAVA
                     AHEAGAAIVHLHVRNEQGKVTCDPARYAKARDLIRAAGSDVVINMSTGGGAGQTTDEQ
                     RMEPISLKPEIASFDCGSCNFGKTVFINSPSFLDELACRMNEHGVLPEIECFEPGHVA
                     NAIRLIDEGKLKPPHWFQVVLNVRGGSPGTIKQLLNMVEMLPAGALWSVCAIGRAQLP
                     INVAAIAMGGHVRTGLEDNIYYHKGQLAESNAQLVARLVRIAGEMGRPIATSAQAREM
                     LGLPAL"
     gene            1507..3000
                     /locus_tag="VFI12_04105"
     CDS             1507..3000
                     /locus_tag="VFI12_04105"
                     /inference="COORDINATES: protein motif:HMM:NF019011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HET7055620.1"
                     /translation="MSRLRIVAAQIQHETNVFSSVRTDMAAFRRSGLKFGTQVEAERG
                     TNSAFGGFFAAAERHDIELLPVLSVWVTPSGIVTADAINELVSHLTEQLIARHPDGVA
                     LSLHGAMVTEVDRDGDGWILEQVREAVGWDIPIVCELDLHANISTRMIEAVDILVGYD
                     TYPHVDMAERGAEVIDLLVRMLRGEINPAMAMVKPPMLPTSQRMMTAHDPMATIMRRA
                     HEIEADPRVLNVTFAGGFPPSDAEEGGVSCVVTTNADLTLAVDYAEDLARLAWTVRDG
                     FLGGVSSFDDAAKAIAALPEQPDTPLLIVDIADSIWSGSSGESVELVRFFLDQGVSGA
                     AVAPIVDPEVVKAAELAGHDTEIEIELGGKTDDLHGPPLVCRARVLALTDGRYVNSGP
                     MMTGLQVDMGPTVVLAIGGPAVRVVVTSFPEAPIDPAVFTANGIDLATTRVLGLKGKG
                     HFRAAFEPVTSDVILVEGPGVTGSDLTRLPLRHTRRPIWPLDDVSFD"
BASE COUNT          572 a          988 c          952 g          507 t
ORIGIN      
        1 gttcggcacc tgttgcagga agatgatgat tgcgatgccg accgtgaacc cttcgaagac
       61 cggccacggc atgtactcga ggtagcgacc gagccgcagc acgcccccga gcatgatcat
      121 cacgccggcc atgatgctga ccgcgaacac cgcgtcggtt ccgtaatgcg ccacgatcgg
      181 gaccagcacg acggtcatcg caccagtcgg gccggagacc tgcacatgcg agccaccgaa
      241 gacggccgca acaagacctg ccacaacggc cgtgaccaaa ccgctcgcgg ccccgagccc
      301 ggtcgtgatt ccaaacgcga gcgccagcgg cagcgcaacg acacccacgg tcaggccggc
      361 gaccaaatcg gccttccagt tggcgcgaat gtttgggtaa tcagacagtc gtggaagcaa
      421 gccggtgagg ttgccaggca agtgcacgga ttcgattcac tttcaacgaa gacggaagac
      481 aggatgcagc ggccggcgat cccgttatcg ccaaaggcct caagcgtgtt catagcatat
      541 tatcgactta tgcgcatgag gcaatccagt ccattcgtgg gattcccgat ggcaatcgcc
      601 aggactgacg gcggtgatag actcgcgcga ccagtaaatt gccccacagg aggtcccttg
      661 cgtgtcgatc aacccgaccg atctcagtca accggtcatc atctcggcag ccctgaccgg
      721 ctcttggccg accaaggccg ataacccggc cgttccgatc accgaggaag aaatcgccga
      781 ggcggccgtt gcggcacatg aagccggcgc ggccatcgtc cacctgcacg tccgcaacga
      841 gcagggcaag gtcacctgcg atccggcccg gtatgcaaaa gcgcgggatc tgattcgggc
      901 agccggctcc gatgtcgtaa tcaatatgtc caccggcggc ggggcggggc agacgaccga
      961 cgagcagcgg atggaaccga tctcgctcaa accggagatc gcctcgttcg actgcggctc
     1021 atgcaacttc ggcaagacgg ttttcatcaa ttcgccgtca ttcctcgacg agctggcctg
     1081 ccggatgaat gaacacggcg tgttacccga gatcgagtgc ttcgaaccgg gtcacgtcgc
     1141 gaatgcgatc cggctgatcg atgagggcaa gctcaaaccg ccgcactggt ttcaggtcgt
     1201 cctgaacgtc cgcggaggca gcccgggcac gatcaagcag ctcctgaata tggtcgagat
     1261 gctgccggcc ggcgcactct ggtcggtctg cgcgatcggg cgggcgcagc tgccgatcaa
     1321 cgtcgcggcg atagccatgg gcggtcatgt ccggaccggg ctcgaggaca acatctatta
     1381 ccacaaaggc caactcgccg agagcaacgc gcagcttgtc gcccgcctcg tccggatcgc
     1441 cggcgagatg ggccgcccga tcgcaacatc cgcccaagcc cgcgaaatgc tcggtttgcc
     1501 ggccctatga gccggttgcg gatcgtcgcg gcccagatcc agcacgagac gaatgtcttc
     1561 agctcggttc gaaccgatat ggccgcgttc cggcgctccg ggctcaagtt cggtacgcag
     1621 gtcgaagccg agcgcggcac caattcggcc ttcggcggct tcttcgcggc ggccgagcgt
     1681 cacgacatcg agttgttacc ggtcctgtcg gtctgggtga cgccatctgg catcgtcacc
     1741 gcggacgcaa tcaatgaact ggtctcgcac ctgactgagc agctgattgc gcgtcatccc
     1801 gacggggtgg cactctccct gcatggtgcg atggtcaccg aggtcgacag ggacggcgac
     1861 ggctggattc tcgagcaagt ccgggaggcg gtcggctggg acatcccgat cgtctgtgag
     1921 ctcgacctcc acgccaacat ttcgacccgg atgatcgaag cggtggatat cctcgtcgga
     1981 tacgacactt acccgcacgt cgacatggcc gagcgagggg ccgaggtgat cgacctgctg
     2041 gttcgaatgc tgcgcggcga gatcaacccg gcgatggcaa tggtcaaacc gccgatgctg
     2101 ccgaccagcc agcgcatgat gaccgcgcac gacccgatgg cgacgatcat gcgccgcgct
     2161 catgagatcg aggctgaccc tcgcgtactg aacgtcacgt tcgccggggg atttccgcca
     2221 tccgatgccg aagaaggcgg tgtgagctgc gtcgtcacca cgaatgcgga cctgacgttg
     2281 gcggtcgact acgcggagga cctcgcccga ctcgcctgga ctgtgcgcga cggattcctt
     2341 ggcggcgtga gcagttttga cgacgcggcc aaggcgatag cggcattgcc agagcaacca
     2401 gacacgccgc tcctgatcgt cgacatcgcc gatagcatct ggtccggcag ctcgggcgag
     2461 agcgtcgagc tcgtccgctt cttcctcgac cagggagtct cgggggcggc cgttgcgccc
     2521 atcgtcgacc ccgaggtcgt gaaagcagcg gaactggccg gccacgatac tgagatcgag
     2581 attgagctcg gcggcaagac ggacgacctg catgggcctc ccctcgtctg ccgcgcgcgg
     2641 gtactcgccc ttaccgacgg acgttacgtc aactccggac cgatgatgac cggcttgcag
     2701 gtcgacatgg gaccgaccgt cgtcctcgcg atcggcggtc cggcagttcg cgtcgtggtc
     2761 acgtccttcc ccgaggcgcc gatcgatccg gccgtcttta ccgcgaacgg catcgacctc
     2821 gccacgaccc gcgtgctcgg cctcaagggc aaaggccatt tccgggctgc gttcgagccg
     2881 gtcaccagtg atgtgatcct cgtggaaggg ccgggcgtga ccggctccga cctgacccgt
     2941 ctgccgcttc gccacacccg ccgcccgatc tggccgctcg acgacgtctc gttcgactga
     3001 gcgcgctggc gcgggctct
//
LOCUS       DASSGX010000236         3456 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_44611, whole genome shotgun sequence.
ACCESSION   DASSGX010000236 DASSGX010000000
VERSION     DASSGX010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44611"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VFI12_04110"
     CDS             <1..214
                     /locus_tag="VFI12_04110"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HET7055621.1"
                     /translation="TRSYGQIATQLGDPSKARAVGWANAANPIPLVIPCHRVIGANGG
                     LTGFGGGIDAKIQLLAHEGAMLPGFG"
     gene            complement(603..1193)
                     /locus_tag="VFI12_04115"
     CDS             complement(603..1193)
                     /locus_tag="VFI12_04115"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET7055622.1"
                     /translation="MRKLVVSEFVTLDGVMQAPGGVDEDTEGGFAHGGWTMPYWHDDI
                     GAHFFAAISEADALLLGRRTWQIHGGAFETMDGDPFADVLNGLRKYVVSTTLPDASAW
                     RNSTLISGNVVGEIRKLKEQPGKTIYVDGSSRLVHTLAENDLVDEYSLLVYPLSLGSG
                     KKVFPDGTRVNLRLIEAHPYPSGVVLMRYVPDRAEQ"
     gene            complement(1450..2826)
                     /locus_tag="VFI12_04120"
     CDS             complement(1450..2826)
                     /locus_tag="VFI12_04120"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HET7055623.1"
                     /translation="MANVIMPKMGDAMEEGTLLRWLKQVGDQIEVGDPIAEIETDKVS
                     LEIEATEGGVLTKTLVDEGQAVAIGTPIATIGAEGETAEAEPEPVPAQAEAPASPAGE
                     NAVTAQESQPVAAQQPINQPEPAAAGAGTTTATPPPAGTNGAGDATAISREPGERLRA
                     SPIVKRLAAEHGIDLNAIAGSGPGGRIVKDDVMPYVTGAKQAPAAPQPAQQVAPPVAP
                     QQAAPAAAGAPGGVARAMPRIRKITGERMFESKDTIPHFYVSSVVEMDAAAAFREQIN
                     AQAKDDESKVSFNDLIVKAAALALREFPNLNTSLEGDTLYDHANIDINIAVAIEGGLI
                     APFVRDADSQSLGAIARTTKDIVGRARKGGLEPSEYQGGTFTITNLGMYDVSEFIAII
                     NPPQAAILAIGSIAEMPVVKDGRVTVGRQMPITLSADHRITDGAEVAKFLIAVKGYLQ
                     NPMQLALS"
     gene            complement(2830..>3456)
                     /locus_tag="VFI12_04125"
     CDS             complement(2830..>3456)
                     /locus_tag="VFI12_04125"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HET7055624.1"
                     /translation="GGGVQLSAQHTHSVEGFYGQFPGLKVVSPVTPYDAKGMMLTAIR
                     DPDPIIFLEAGALYGTKGEVPEGEYTVEFGKARIAKEGTDISLISYGRQVNLLLRVAD
                     ALDKEQISAEVIDLRSIRPFDVDTIIDSVKKTNRAVVVQEQWRWFSVASEVAALIQEH
                     AFDYMDAPVERVSGAEVPAPYSKSLEAAAFPSEQDVIEAAKRALYRKA"
BASE COUNT          519 a         1163 c         1137 g          637 t
ORIGIN      
        1 gacgcgcagt tacggccaga tcgcaaccca gctcggcgac ccttcgaagg cccgggcggt
       61 cggctgggcg aacgccgcca acccgatccc gctcgtcatc ccctgccacc gggtgatcgg
      121 cgccaacggc ggcctgaccg gcttcggcgg cggcatcgac gccaaaatcc agctcctcgc
      181 ccacgaaggc gccatgcttc cggggttcgg atagcgggtt gagggtggag ggttaaggga
      241 acgcgtgtag gggcagatgg catctgcccg ccgctccgtg atagcccggt tgcatgcacc
      301 cgaaccaaaa cgtaacgcgg tcccccgtgg gccgctcgca cccgccgccc gacgtgatcg
      361 gtggcgggaa ccgagcccgg ctcccggtcc accgccatct cccttacgcg gggatgggcg
      421 ccacgcgggg cttacgcacc gcgctacgtt cggacgcatg gtgtcgcacc gcttggattc
      481 agcccgggtg tttacgcccg ggcgatcccg ggtacgaacg cccatatgcc accgggtcct
      541 cggacaacgg agggcagatg ccatctgccc ctacaatgtt ccgcgccttc gcgcctcgat
      601 ccctactgct cggctcggtc cggcacatac ctcatgagca ccacgcccga tggatacggg
      661 tgcgcctcga tcaggcggag gttgacccgg gtgccgtcgg ggaagacctt cttgccgctg
      721 ccgagcgaga gcgggtagac gagcaggctg tactcgtcga ccaggtcatt ctcggccagg
      781 gtgtggacca gccggctgct gccgtcgacg tagatcgtct tgcccggctg ctccttcagc
      841 ttgcgaatct caccaaccac attgcccgag atcagggtcg agttgcgcca ggctgaggcg
      901 tcgggcaggg tcgtcgagac gacgtacttc cgcaggccgt tcagcacgtc ggcgaaggga
      961 tcgccgtcca tggtttcgaa tgcgccgccg tggatctgcc acgtcctgcg gccgagaagc
     1021 agggcatcgg cctcgctgat cgccgcgaag aagtgcgcgc cgatatcgtc atgccagtag
     1081 ggcatcgtcc acccgccgtg ggcgaagccg ccttcggtat cctcatccac gccgcccggc
     1141 gcctgcatca ccccatccaa cgtcacgaac tcggaaacca ctagtttgcg cattgctatc
     1201 ttcctttcgc gttgttcgta gctttccacg tcaccacatc cagtagtcgt acggcatcat
     1261 tcaggttcga catcgatgct gggcggctgg gccgttgtag ggatttgcgg gtgcaaatcc
     1321 gccggcagcc gggacaggcc ttggatgtgg cacgcgaggc ggcaaggcgg gtcgcattgc
     1381 catgcgacgc tacacgggaa cggccgggcg aattcgcccg gccgtcgttc gtaactgctc
     1441 gccttcgcct caggaaagcg ccaactgcat cgggttttgc agatagccct tgaccgcgat
     1501 caggaacttc gcgacttcgg cgccgtccgt gatgcggtga tcggccgaga gggtgatcgg
     1561 catctgccgg ccgaccgtga cgcggccatc cttgaccacc ggcatctccg cgatcgagcc
     1621 gatcgcgagg attgcggcct gcggcgggtt gatgatcgcg atgaactcgg agacgtcgta
     1681 catgccaagg ttcgtgatcg tgaacgtgcc gccctgatac tccgacggtt ccagaccgcc
     1741 cttccgcgct cgaccaacga tgtccttggt cgtccgcgcg atcgcgccga ggctctggct
     1801 atcggcatcg cgtacgaacg gggcgatcag accaccttcg attgcgacgg cgatgttgat
     1861 gtcgatgttg gcatggtcgt agagggtgtc accttcgagg ctcgtgttca ggttcgggaa
     1921 ctcgcgcagg gcgagggccg ccgccttgac gatcaggtcg ttgaaggaga ccttgctctc
     1981 gtcgtccttc gcctgggcat tgatctgctc gcggaaggct gccgccgcgt ccatctcgac
     2041 cacggagctg acgtagaagt gcggaatggt gtccttggac tcgaacatcc gctcgccggt
     2101 gatcttccgg atgcggggca tcgcccgcgc gactccaccc ggcgccccgg ctgctgccgg
     2161 agccgcctgc tgtggcgcga ccggaggggc aacctgctgg gccggctgcg gtgcggccgg
     2221 agcctgcttg gcgccggtca cgtacggcat gacgtcgtcc ttgacgatcc tgccgcccgg
     2281 accagacccg gcgatcgcgt tcaggtcgat gccgtgctcg gcggcgaggc gcttgacgat
     2341 cggggaggcg cgcaaccgct cgcccggctc ccgggagatg gcggtggcat cgcccgcgcc
     2401 gttggtcccg gccggtggcg gggtcgcggt ggtcgttccg gcgccggctg cggcgggctc
     2461 gggctggttg atcggctgct gcgcggcgac cggctggctc tcctgcgccg tgaccgcatt
     2521 ctcgccggcg ggcgaggccg gcgcttcggc ctgggccgga accggctcgg gttccgcctc
     2581 ggccgtctcg ccctcggcgc cgatggtcgc aatcggggtg ccgatcgcca cagcctggcc
     2641 ctcgtcgacc aaggtcttgg tcaggacgcc gccttcggtc gcctcgatct cgagcgagac
     2701 cttgtcggtt tcgatctcgg cgatcgggtc accgacttcg atctggtcgc cgacctgctt
     2761 gagccagcga aggagtgttc cctcctccat ggcgtcgccc atcttgggca tgatcacatt
     2821 tgccatcggt taggcctttc gatagagcgc ccgtttggcg gcctcgatca catcctgctc
     2881 gctcgggaat gcggcggcct cgagcgattt cgagtacggc gccgggacct ccgcgccgct
     2941 cacccgctcg accggggcat ccatatagtc gaaagcgtgt tcctggatca gcgcggcgac
     3001 ttcgctggcg acgctgaacc agcgccactg ctcctgcacg accacggccc ggttcgtctt
     3061 cttgaccgaa tcgatgatcg tgtcgacatc gaacgggcgg atcgagcgaa ggtcgattac
     3121 ctcggccgag atctgttcct tgtcgagcgc atcagccacc cgcagcagca ggttgacctg
     3181 ccggccgtag ctgatcagcg agatgtccgt gccctctttc gcgatccggg ccttgccgaa
     3241 ctcgacggtg tactcgccct ccggcacctc acctttggtt ccatagagcg cgcccgcctc
     3301 gaggaagatg atcggatcgg gatcgcggat cgccgtcagc atcatcccct tagcgtcata
     3361 gggtgtcacc ggagagacga ccttcaggcc cgggaactgg ccatagaaac cctcgacgct
     3421 gtgcgtgtgc tgggccgaga gctggacgcc gccgcc
//
LOCUS       DASSGX010000237         2741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_371512, whole genome shotgun sequence.
ACCESSION   DASSGX010000237 DASSGX010000000
VERSION     DASSGX010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371512"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="VFI12_04130"
     CDS             <1..698
                     /locus_tag="VFI12_04130"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7055625.1"
                     /translation="ENRYREALVVYRQLEDRSGTAQTLHNLAEVLRDRGDYPEAISAW
                     QESLALRSVQGDRVGVAECLTGLARVATAAGLHDFAARLFAAAIRLQAETGVSLPPNE
                     RDAQEKAIAELRRVSPAADFQRSWDTGTHATLQSLLEEVANSYETLTDASTRVAAKPS
                     TTKTAAAEAGLTRREVDVLRLVVDGLSDREIGDALFISHRTAMTHVANILGKLGLESR
                     TAAAAHALRNNLI"
     gene            998..>2741
                     /locus_tag="VFI12_04135"
     CDS             998..>2741
                     /locus_tag="VFI12_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055626.1"
                     /translation="MSTQSTRYSRTRQHGGLVRALFAAVLLAMLVMPLTDARVGAKDL
                     HQINPIQIGDQPIVDPGTVVDPPVVDPPVDTPTPAPTQPPVQQVPVDSPVLDDPVVLD
                     PGSQVNDLYINPVLCPASLDLSAVDYYGLAANCNLGYANGWELIVMDSQSNWWIHNVI
                     NGSGVGQGGLPNDDYYMSFTHPASGFSPHRIVCQAHDQLGNDAVAPFDLVTDDYGGTV
                     SVSGGLMWFCDAFIKVDGPAAAPIDVLINKHGCPQGIHSDDYYFLASMCQDMLTGINF
                     TLTDGASQTYTQTTAGAPAMATFSQVTGGNVSVAESIPGGYDNPVVFCGVKDLLGNDL
                     ESSFRNPAVTNGAFILDSLPADTATVFCDVYNIPTDSDGGSIIIVKRFCPVDYDIQNG
                     NPTSDCGQPQDGVQFNVTGPNGYASQSNTGDSMSSAVSFGGLEAGEYQVAETLPAGVG
                     SAWIWSCSSDTADLSGFAPADVTGSPFTYDLADNEDILCFWYNVPSSDGSVTIHKWEC
                     PEGVELGKDASYYLTTCATAMAGVEFGESKLGDTAQIVSTDASGTVTFSVGANVDWVI
                     EEHVPSGYGDPIVFC"
BASE COUNT          472 a          994 c          802 g          473 t
ORIGIN      
        1 ccgagaaccg gtatcgcgag gcgctcgtcg tttaccggca gctcgaggac cgctccggca
       61 ccgcccagac gctgcacaac ctggccgagg tcctgcgcga ccgcggcgac tacccggagg
      121 cgatctccgc ctggcaggag agccttgctc tgcggtccgt gcagggtgac cgggtcgggg
      181 tcgccgagtg cctgaccggc ctcgcgcggg tcgccaccgc cgccggcctg cacgacttcg
      241 cggcgcgctt gttcgccgcg gcgatccggc tccaggccga aaccggcgtc tcgctgccgc
      301 ccaacgagcg cgacgcccag gagaaggcga ttgccgagct aaggcgcgtc tcccccgccg
      361 ccgatttcca gcgctcatgg gacaccggga cacacgcgac gctgcagagc ctgctcgaag
      421 aggtcgcgaa cagctatgaa accctgaccg acgcatccac ccgggtggcc gcaaaaccgt
      481 cgaccacgaa aacggccgcc gccgaagccg gactgactcg ccgcgaagtc gatgtccttc
      541 gtctggtcgt cgacggtctc tcggatcgcg agatcggcga cgcactcttc atcagccacc
      601 gcaccgccat gacccacgtt gccaatatcc tcggtaaact cggcctcgaa agccgaacag
      661 ccgccgccgc ccacgcgctc aggaataacc tgatctgacg ggggttttcg gctttggtct
      721 ctcgtctttg gtctttcgtc attcgttttc ggttttccgt tttccgatcc gtcaacttcg
      781 cgcctccgcg cctccgcgcc ctgctgctcg actcttcgcc tgctcgcctg ctcgcctgct
      841 cgcctcacct acgtgatccc acgtagtcgc tccgcctacc tcaccagcat gcaaatacgt
      901 gaccttcccg atgtcccggt gtgccttgaa accgaccatg aaggtgtctt cggacggttg
      961 gttttcagcg cggtggcgca caggagagga cacgacgatg agcacgcagt cgacgcgata
     1021 ttcccggacc cggcagcatg gaggtctagt acgagccctc ttcgccgccg ttctgctcgc
     1081 catgctggtc atgccgctga ccgacgcccg tgtcggcgcg aaagacctgc accaaatcaa
     1141 tccaatccag ataggggatc agccgatcgt cgaccccggc acggtcgtgg acccgccggt
     1201 ggtcgatcct ccggtggata ctccgacccc ggctccaact cagccgcccg tgcagcaggt
     1261 ccccgtcgac tccccggtcc tcgacgatcc ggtggtcctg gaccccggct cgcaggtcaa
     1321 cgacctctat atcaacccgg tcctctgccc cgccagcctg gatctgtcgg cggtcgatta
     1381 ctacggcctc gccgccaact gcaaccttgg ttacgcgaat ggctgggaac tgatcgtgat
     1441 ggacagccag tccaattggt ggatccataa cgtcatcaat ggcagcggcg tcggccaggg
     1501 cggtctgcca aacgacgact actacatgtc gttcacccac ccggccagcg gattcagccc
     1561 gcaccggatc gtctgccagg cccacgatca gctcggcaac gacgcggtcg cgccgttcga
     1621 cctggtaacc gacgactacg gcggaacggt ctcggtctcc ggcggcttga tgtggttctg
     1681 cgatgccttc atcaaggtcg atggccccgc tgccgcgccg atcgatgtcc tcatcaacaa
     1741 gcacggctgc ccgcaaggca tccacagcga cgactactac ttcctcgcct cgatgtgcca
     1801 ggacatgctg accggcatca acttcaccct gaccgacggc gcgagccaga cctacaccca
     1861 aaccacggcc ggcgcgccgg caatggcgac cttctcccag gtcacgggcg gcaatgtctc
     1921 ggtcgcggaa tccatcccgg gcggctatga caatccggtc gtcttctgcg gcgtcaagga
     1981 cctcctcggc aacgacctgg agagcagctt ccggaacccg gcagtcacga acggcgcctt
     2041 catcctcgac agcctgccgg ccgacacggc gacggtcttc tgcgacgtct acaacatccc
     2101 taccgactcc gacggcggct cgatcatcat cgtcaagcgc ttctgcccgg tcgactacga
     2161 catccagaac ggcaacccga cctccgattg cggccagccg caggacggcg tccagttcaa
     2221 cgtgaccggc ccgaacggct atgcctcgca gtcgaacacc ggcgactcga tgagcagcgc
     2281 ggtctccttc ggcggcctcg aggcgggtga gtatcaggtc gccgagaccc tgccggccgg
     2341 cgtcggttcg gcctggatct ggtcctgctc gagcgacacg gctgacctga gcggcttcgc
     2401 tccggccgac gtgaccggct cgcccttcac ctacgacctg gccgacaacg aggacatcct
     2461 ctgcttctgg tacaacgtcc cgtccagcga cggctcggtc acgatccaca agtgggagtg
     2521 cccggagggc gtcgagctcg gcaaggatgc cagctactac ctgaccacct gcgccacggc
     2581 gatggcggga gtcgagttcg gcgaaagcaa gctcggcgac accgcgcaga tcgtctcgac
     2641 cgatgccagc ggcaccgtca ccttctcggt cggcgccaac gtcgactggg tgatcgagga
     2701 gcacgtgccc tccggctacg gcgatccgat cgtcttctgc a
//
LOCUS       DASSGX010000238         2014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1241969, whole genome shotgun sequence.
ACCESSION   DASSGX010000238 DASSGX010000000
VERSION     DASSGX010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1241969"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..1650
                     /locus_tag="VFI12_04140"
     CDS             157..1650
                     /locus_tag="VFI12_04140"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HET7055627.1"
                     /translation="MKHIKSLREFVDDLDAVGEIQRIDTEVDWNLELGAIIRRSYDLR
                     APAPLFTNITGYQDTGFRVLGAPGSLSGPDHRLARLALAVGLPADATGQQIVQALADA
                     RSRPGIRPVEVPAAEAPCKQNIMTGEDIDLWAFPTPFLHGNDGGRYIQTYGMNIVRTP
                     DGSWTNWSINRMMVAGRDTLACLIPAPQHLGIIRAKWTGIGEPMPIALAIGVEPALPL
                     VGGMPLPEGADESHYVGALFGEGIEVVRAETVDLMVPATAEIVIEGHIDPDRTVMEGP
                     FNEFPGYNATESSPKQLFTVSAVTYRNRAIMPAVAAGPPVEEDHTIIGTTSAAESLHL
                     VRRAGLPVSSAWYNFEAAVHWLVLALHADWHEATGLHARELADRVAEVVFAGKPGVNA
                     PKLMLVEDDIDITDLEQVMWAFATRCHPDVDRGAFHYPNKVSDQLAVYLSAAEQHAFL
                     AGKVVYNCLLADLYPERRRPVKGSFENGWPREVQQQVLSNWQSYGYR"
     gene            1822..>2014
                     /locus_tag="VFI12_04145"
     CDS             1822..>2014
                     /locus_tag="VFI12_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055628.1"
                     /translation="MSRIELDRCLGCSARLGCGGARAARASGLGQRGLDIGASALRAL
                     DQAGLVGEDHGLDAVAQAEL"
BASE COUNT          319 a          738 c          663 g          294 t
ORIGIN      
        1 accggtcagc agatcgtgca ggccttggcc gacgcgcgct cccgccccgg catccggccg
       61 gtcgaggtcc cggcgccccg cgctggggtg acgacaccgg cccgactgac tctgactgtt
      121 tgacagcacc atccgaatcg acccgaaagg ccaccgttga aacacatcaa gagtctgcgc
      181 gagttcgtcg atgacctgga cgcggtcggc gagatccagc gcatcgacac cgaggtcgat
      241 tggaacctcg agctgggtgc gatcatccgc cgctcctacg acctgcgcgc tcccgccccg
      301 ctgttcacca acatcaccgg ctaccaggac accgggttcc gggtgttggg ggcgcccggg
      361 tcgctcagcg gaccggatca ccggctcgcc cggctcgccc tggccgtcgg cctccccgcc
      421 gacgcgaccg gtcagcagat cgtgcaggcc ttggccgacg cgcgctcccg ccccggcatc
      481 cggccggtcg aggtcccggc cgccgaagcg ccctgcaagc agaacatcat gaccggcgag
      541 gacatcgacc tctgggcgtt ccccaccccg ttcctgcacg gcaacgacgg cggtcgctac
      601 atccagacct atggcatgaa catcgtgcgg accccggacg ggtcctggac caactggtcg
      661 atcaaccgga tgatggtcgc cggccgggac acgctggcct gcctgatccc cgcgccccag
      721 catctgggca tcatccgagc caagtggacc ggcatcggcg agccgatgcc gatcgcgctc
      781 gccatcggag tcgaaccggc gctgccgctg gtcggtggga tgccactgcc ggagggcgcc
      841 gacgagagtc actacgtagg tgcactcttc ggggaaggca tcgaggtggt ccgggccgag
      901 acggtcgatt tgatggtccc ggccacggcg gagatcgtga tcgagggtca catcgaccct
      961 gaccgaaccg tgatggaggg cccgttcaac gagtttcccg gctacaacgc gaccgaatcc
     1021 tcccccaagc agctgttcac ggtctcggca gtcacttacc gaaaccgcgc catcatgccc
     1081 gcggtcgcgg ccggaccgcc cgtcgaggag gaccacacga tcatcggcac caccagcgcc
     1141 gcggagagcc tccacctggt ccgccgcgcc ggactgccgg tctcatcggc gtggtacaac
     1201 ttcgaggctg cggtccactg gctcgtcctg gccctgcacg ccgactggca cgaggccacc
     1261 ggtctgcacg cccgcgaact cgcggaccgg gtcgccgagg tggtcttcgc cggcaagccg
     1321 ggcgtcaacg cgcccaagct catgctggtc gaggacgaca tcgacatcac cgacctcgag
     1381 caggtcatgt gggcattcgc cacccgctgt caccccgacg tcgatcgcgg cgcgttccac
     1441 tacccgaaca aggtcagcga tcaactcgcc gtctacctgt cggccgcgga gcagcatgcc
     1501 ttcctcgccg gcaaggtcgt atacaactgc ctgctcgccg atctctaccc cgagcggcgg
     1561 cgcccggtga agggcagctt cgagaacggc tggccccgcg aagtacagca gcaggtcctc
     1621 tccaactggc agagctacgg ctaccgctga ctgaccgtag gccggatgct ggcgccgttt
     1681 ccgcgcgccc ggcgcgaccg cgcgcgcggt cggctccggc gacgacccca ggcgccgcac
     1741 cgtgcggtcc gcggcgcgca cgtgtacgtg tacgcagcgg tcgctgctcc cgcggcgccc
     1801 tccggttcgc ggcgccgctg gatgagccgg atcgagctgg accgctgtct tggctgctcg
     1861 gcgaggctgg ggtgcggcgg cgcccgagcg gcccgagcat ccggcctcgg gcaaagaggc
     1921 ctggacatcg gagcgtcagc gctgcgagcc ctggaccaag ccggtctcgt aggcgaggat
     1981 cacggcctgg atgcggtcgc gcaggccgag cttg
//
LOCUS       DASSGX010000239         2041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1352154, whole genome shotgun sequence.
ACCESSION   DASSGX010000239 DASSGX010000000
VERSION     DASSGX010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352154"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="VFI12_04150"
     CDS             complement(<1..748)
                     /locus_tag="VFI12_04150"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HET7055629.1"
                     /translation="MASTLSTTDTLSKEQILHLYREMVNIRRTEEQLAKSYQAGNIPG
                     ACHTYIGQEAVAVGVCAYLRPNDVIFSTHRGHGHALAKGVPPRQVIAELFGKATGCSR
                     GRGGSMHLFAPETGLMGTTGIVGPNILQATGAGYSFKLLRSDRVSVGFFGDGAVSNGA
                     FHEGINMAAIWDLPVIYVCENNMYATEVAFAYATKNQDVAERARSYGIPGVQVDGNDV
                     LAIHEAAGAAIERARAGHGPTLIEARTYRRG"
     gene            complement(749..1903)
                     /locus_tag="VFI12_04155"
     CDS             complement(749..1903)
                     /locus_tag="VFI12_04155"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HET7055630.1"
                     /translation="MTLDTLERAQSAIREAGIDIALVSSIANVTYVTGVEVPLPVGAG
                     YEMAYAPWLAVLTVNGAGLVSGGEAGGMGVAGHGFDLFTIEGFDHDRATDPAGSYAGR
                     LKAALIASGVTGGSGTIGVELRSLSFVAGQIIRAALPNWTIVDIEPAMIRARWIKTPR
                     EIELLRTASAVSDVAQRTLAELVRTSGRSEINMYTEITRRTFEAAGGAIQLAGELVTG
                     PRTTVVAYPAGPKCRTTEPGDAALMDLSGRVNGYWFDCTNTHVIGGAPPTEEQRRYAK
                     ASQAACEAGMAALKPGNKASDASRAAAAAFASFGLANAHYTGHQIGVTVNELPRLVPY
                     DDSIIEPGMVFSVEPGAYQGPGGTFGSRSEKMVLVRADGPEILSQFDWGI"
BASE COUNT          318 a          667 c          687 g          369 t
ORIGIN      
        1 cgcctcggcg ataggtgcgg gcctcgatca gggtcggacc gtgcccggcg cgggcccgct
       61 cgatggccgc gccggcggcc tcgtggatcg cgagcacgtc gttgccgtcg acctgcacgc
      121 ccgggatgcc gtaactccgc gctcgctcgg cgacgtcctg gttcttcgtc gcgtaggcga
      181 aggcgacctc ggtcgcgtac atgttgttct cgcagacgta gatgaccggc agatcccaga
      241 tggcggccat gttgatgccc tcgtgaaagg cgccgttgct gaccgcgccg tccccgaaaa
      301 agccgacgct aacccggtcg gacctcagca gcttgaagct gtatccggcg ccggtcgcct
      361 ggaggatgtt cgggccgacg atgccggtcg tgcccatcaa gccggtctca ggcgcaaaga
      421 gatgcataga gccaccccgg ccgcgcgagc aaccggtcgc cttcccgaac agctcggcga
      481 tgacctggcg cggcggcacg cccttggcga gtgcgtgccc gtggccacga tgggtgctga
      541 agatgacatc gttcgggcgc aggtaggcgc agacgccgac cgctaccgct tcctgcccga
      601 tgtaggtgtg gcaggcgccg gggatgttgc cggcctggta actcttggcg agctgctcct
      661 cggtgcggcg aatgttgacc atttcgcggt agagatggag gatctgctcc ttggagagtg
      721 tgtcggttgt gctcagcgtg gacgccatct agatacccca gtcgaattgc gacaaaatct
      781 ccggcccgtc ggcgcggacc agcaccatct tttcggagcg ggagccgaac gtgccgcctg
      841 gtccctggta ggcgcccggc tcgacactga aaaccatccc tggttcgatg atcgagtcgt
      901 catacggcac gaggcgcggc agctcgttga ccgtgacgcc gatctggtgg ccggtgtaat
      961 gggcattcgc caggccaaac gaggcgaagg cagcggcagc ggctctcgat gcgtcgctgg
     1021 ccttgttacc gggcttgagc gcggccatcc cggcctcgca ggcggcctgg ctcgccttcg
     1081 cgtagcggcg ctgttcctcg gtcggcgggg cgccgccgat gacatgcgta ttggtgcagt
     1141 cgaaccagta gccgttgacg cggccactca ggtccatcag cgcagcgtcg cctggctcgg
     1201 tcgtgcggca tttcggaccg gccggatagg ctacgacggt cgtgcgtgga cccgtgacca
     1261 gctcgccggc cagttggatc gcaccgccgg ccgcctcgaa ggtgcggcgg gtgatctcgg
     1321 tatacatatt gatttcggat cgcccggagg tgcggaccag ttcagccagt gtccgctgcg
     1381 cgacatcgga aacggccgac gccgtgcgta gcagctcgat ttcgcgcggt gttttaatcc
     1441 agcgggccct gatcatggcc ggctcgatat caacgatcgt ccagtttggc agtgccgcgc
     1501 gaatgatctg gccggcgacg aacgagagcg atcgcaattc gacgccgatc gtgccggatc
     1561 ccccggtcac acctgaggca atcagcgcgg ccttcaaccg accggcgtag ctgccggctg
     1621 gatcagttgc gcggtcatgg tcgaatcctt cgatcgtgaa caggtcgaac ccgtgcccgg
     1681 cgacacccat tccgccggcc tcgccccccg agacgaggcc ggcgccattg accgtgagca
     1741 ccgccagcca cggcgcgtac gccatctcgt atccagcgcc gacggggagg gggacttcga
     1801 cgccggtgac gtaggtcacg tttgcgatcg acgagacgag ggcaatgtca atccctgcct
     1861 cccggatcgc cgattgtgct cgctcgagcg tatcgagtgt catgcctgcc gttcctagtt
     1921 ggccgtcacg gcgaccgtgg cgacctcgtt tgccgggtac ttcggccgct gtcgttgcaa
     1981 caccagaacc acgtcctggg ctccgcgttc ccggacgtcg tagccggact cggtcgaggc
     2041 c
//
LOCUS       DASSGX010000240         3752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_45642, whole genome shotgun sequence.
ACCESSION   DASSGX010000240 DASSGX010000000
VERSION     DASSGX010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45642"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..859
                     /locus_tag="VFI12_04160"
     CDS             <1..859
                     /locus_tag="VFI12_04160"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HET7055631.1"
                     /translation="PHRAAVRLASTQESPRMSPSLDRRRALVSLAGIGAAGITTTRVA
                     MAQGTPPAESAEAVGTVLVVSATGSASAPATHAIAQVVIRANSGAEPVPDPSGTPGSA
                     APVVTDEDMQAVVDALVAQGVDPSRILTNIAPGGVGGGAFGPGTAAVVFQVNGEQIKT
                     LGRTLEGAVQTVAERGLMFDQPGAMYLADTCQDLRAKAYQDAVAEGMAEATLLADATG
                     VSLAGLTQAKKQSISYGPAAFGYGQSDSCADLMDLGTAARTYLPAYDATLPNEFIVYA
                     AVELSFATA"
     gene            901..2052
                     /locus_tag="VFI12_04165"
     CDS             901..2052
                     /locus_tag="VFI12_04165"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HET7055632.1"
                     /translation="MITHDEILARQERAREAAEAAGYDALLTIGRSFYDRPGDLAYLT
                     NHFPPFPSTVFTGEHRGLGHALFLLPVLGAAALVTDPRRYRADLVPVEDVRPSSNLMA
                     TTIDLLRERGLERAKVGVIGDDIMPAPMYRELREELPELRFMPEPDLLCRMRMIKSPA
                     EQDAMRKAAKAADAALQAAIATIQRGRATERSACAAGIFAAMQAGADFVRYMRVHSGP
                     WSALGSRWPQATEREIQRGDLVILDAIGAVEGYQFDVNRSLSAGPTDADRLQLLETVY
                     KALEAAIDACRPGNRVLDVVDAAKGVIDRSPFANAFGGMMGHGIGLETVEEPLLTAGN
                     ETELAPGMVLCVEPGLFVPNWGGALIEQEVIIREAGPPEVITETPAWLW"
     gene            2052..2660
                     /locus_tag="VFI12_04170"
     CDS             2052..2660
                     /locus_tag="VFI12_04170"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HET7055633.1"
                     /translation="MGETTANLPSALAFSRLRQGLHGSRIIVRLMVDSDAEAVFAAID
                     GARSHLKAWMSWVDRHQDVADTRDYVTRQLVEFTQRVNLTLGIFDRADNQTVLGTTGF
                     HNIDWTVPALEIGYWIIPEAQGQGYVTEAVSLLTDFAFAEFGANRISIHCDPRNERSK
                     RVAERLGYHLEGRLRNMTRTPDGSLRDTLVFSLVPGDPRIQR"
     gene            complement(3214..>3752)
                     /locus_tag="VFI12_04175"
     CDS             complement(3214..>3752)
                     /locus_tag="VFI12_04175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7055634.1"
                     /translation="ALAAVPQIDILVNNAGMSIRGNFWDVTDDEWDYQVNVNVRSPFI
                     LAQAVAKHFIEQATRGRIVNISTIGVHACHKDAAVYNLAKAGVEAMTRNMAFELGPYG
                     ISVNCVAPGNIALRPGQAEQPWFADAARAIPYGRVGHAEDIAAAVKFFCLPETGFTTG
                     QTLLVDGAHDSYLPERIP"
BASE COUNT          597 a         1188 c         1322 g          645 t
ORIGIN      
        1 gccacatcgg gcggccgttc gtctggccag cacacaggag tcgccacgca tgtccccttc
       61 cctcgaccga cgccgcgccc tcgtttccct ggccgggatc ggcgcggccg ggatcaccac
      121 gacccgcgtc gcaatggccc agggcacgcc gcccgcggag tcggccgagg ctgtcggaac
      181 cgtgctcgtc gtctcggcga ccggctcggc ctcggctccg gccacgcacg cgatcgcgca
      241 ggtggtgatc cgcgcgaact ccggggcgga accggtcccg gatccatccg ggacacccgg
      301 tagcgccgcg ccggtggtga ccgacgagga tatgcaggcg gtggtggacg cattagtcgc
      361 gcagggcgtg gatccgtccc ggatcctgac caacatcgcg cccggcggag tcggcggcgg
      421 cgcgttcgga ccgggcacgg cagccgttgt ctttcaggtg aacggcgagc agatcaagac
      481 cctgggcagg acgctcgagg gtgcggtcca gactgttgcc gagcgggggc tgatgtttga
      541 ccaacccggc gcgatgtacc tggccgacac ctgccaggat ctccgggcga aggcgtacca
      601 ggatgccgtt gccgagggaa tggcggaggc gacgctgctg gcggatgcga ccggcgtgtc
      661 actggccggg ttgacacagg cgaagaagca gtcgatctcc tacggtccgg ccgcgttcgg
      721 gtacggccag agcgattcct gcgcggatct gatggacctg ggcacggcag cgcgaaccta
      781 cctgccggcc tacgacgcga cgctgccgaa cgagttcatc gtctatgccg ccgtcgagtt
      841 gtccttcgcg accgcctgag gccattcccg tcgcgcgtga tcgttcggta cactgggcga
      901 atgattacgc acgacgagat tcttgcccgc caggagcgcg ctcgcgaggc tgctgaggca
      961 gcggggtacg acgcgctcct gaccatcggc cgctcctttt atgaccggcc gggcgacctc
     1021 gcctacctga ccaaccactt ccccccgttc ccatcgaccg tcttcaccgg cgagcaccgc
     1081 ggcctcggcc atgccctgtt cctgttgccg gtgctgggag cggccgcgct ggtgaccgac
     1141 ccccggcggt accgggccga tctcgtcccg gtcgaggatg tgcggccgtc cagcaacctg
     1201 atggcgacca cgatcgacct gctgcgcgag cgtgggctgg agcgggcgaa ggtcggggtg
     1261 atcggcgacg acatcatgcc cgcgccgatg taccgggagc tgcgcgagga gctgccggag
     1321 ctccggttca tgcccgaacc cgacctcctg tgcaggatgc ggatgatcaa gagcccggcc
     1381 gagcaggacg cgatgcggaa ggcggccaag gcagcggatg cggctctgca ggcggcgatc
     1441 gcgacgatcc agcggggccg ggcgacggaa cgatccgcct gcgccgccgg aatcttcgcc
     1501 gcgatgcagg ccggcgccga tttcgtgcgg tacatgcggg ttcattccgg accgtggtcg
     1561 gcgctcggct cgcgctggcc gcaggcgaca gaacgggaaa tccagcgggg cgacctcgtc
     1621 atcctcgacg cgatcggcgc ggtcgagggc taccagttcg acgttaaccg ctccctgtcc
     1681 gccggcccga ccgatgccga ccggcttcag ctcctcgaaa cggtctacaa ggcactcgag
     1741 gcggcgatcg acgcgtgccg gccggggaac cgggtgctgg atgtggtcga cgcggccaag
     1801 ggcgtgatcg accgcagtcc cttcgcgaac gcgttcggcg ggatgatggg gcacgggatc
     1861 gggctggaga cggtcgagga accgctgttg acggccggga acgagaccga gctggcgccg
     1921 ggcatggtgc tttgcgtgga gccggggctc ttcgtgccca actggggcgg ggcactgatc
     1981 gagcaggaag tgatcatccg ggaggcgggt ccgccggagg tgatcacgga aacgccggcc
     2041 tggttgtggt gatgggggag acgaccgcca acctgccctc ggcgctggcc ttcagccggc
     2101 tgcggcaggg gctgcacggc agccggatca tcgtccggct gatggtcgat tccgatgcgg
     2161 aggcggtctt tgccgcgatc gacggcgccc gcagccatct caaggcgtgg atgagctggg
     2221 tcgaccggca ccaggatgtg gccgataccc gcgattacgt cacccgccag ctggtcgagt
     2281 tcacgcagcg ggtcaacctg acgctcggca tcttcgaccg ggccgataac caaaccgtgc
     2341 tcggcacgac cggctttcac aacatcgatt ggaccgtgcc ggcgctcgag atcggctact
     2401 ggatcatccc ggaggcgcag gggcagggat acgtgaccga ggcggtctcg ctgttgactg
     2461 attttgcctt tgcggagttc ggggcgaacc ggatctcgat ccactgcgac ccgcgcaacg
     2521 agcggagcaa gagggtggcc gagcggttgg gctatcacct cgagggccgg ttgcgtaaca
     2581 tgacgcggac gcccgacggg agcctgcgcg acacgctggt gttctccctg gtgcccgggg
     2641 atccgcggat ccagcggtaa gggatcgaac ggggcgagaa aatggcccca taaacggatg
     2701 cggccgcgcg ggtggctggt cccgcgcggc gcagatcctg aaggggtagg gtcgcttgaa
     2761 cagtggctgg tccgttcaag cggggaatcc tgtacccatg cgactaaggt ggctggtcct
     2821 tgccgcactt cgttgaattc atagtaggcc aggaatgtga agaaaggatg taccactcgg
     2881 atacgtggta cggtacgatc ggactccaga ctacgcgtaa cgaatcgcac gctacacgcg
     2941 ttgcagtgtg aataggccgt tctcgcccgc cggtgtaggc caggccacta ttcccgggtt
     3001 ttaggatttc ggttttgggt tttcggtttt cggttttcgg ttttcggttt tcggttttcg
     3061 gggaagagta tgcccgcggt tgaaaccccg ggctcagaca cgaagcctct ccgaggctgg
     3121 tcgcattgcc atgcgaagct acacctccgc gtccctcttt tcgcctgctc gcctgctcgc
     3181 ctgctcgcct ccgcgcctgc tcgcctcccc gcctcacggg atgcgttccg gtaagtaact
     3241 gtcgtgggcg ccgtcgacga ggagggtttg gccggtggtg aagcccgttt cgggcaggca
     3301 gaagaatttg acggcggcgg cgatgtcctc ggcgtggccc acgcggccat aggggatggc
     3361 gcgggcggca tcggcgaacc acggctgctc ggcctggccg gggcggaggg cgatgttgcc
     3421 cggcgcgacg caattgacgc tgatgccgta cgggccgagc tcgaacgcca tgttccgggt
     3481 catcgcctcg acaccggcct tggcaaggtt gtagacggcg gcgtccttgt gacaggcgtg
     3541 aacaccgatc gtgctgatgt tgacgatccg gccccgggta gcctgctcga tgaagtgctt
     3601 cgccaccgct tgcgccagaa tgaagggtga gcggacattg acgttgacct gatagtccca
     3661 ctcgtcgtcg gtaacatccc agaaattgcc gcgaatgctc atcccggcgt tgttcaccaa
     3721 tatgtctatc tgcgggacgg ccgcaagcgc ct
//
LOCUS       DASSGX010000241         2892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_153762, whole genome shotgun sequence.
ACCESSION   DASSGX010000241 DASSGX010000000
VERSION     DASSGX010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153762"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VFI12_04180"
     CDS             <1..353
                     /locus_tag="VFI12_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016636114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HET7055635.1"
                     /translation="PEFLNRVDELVIFEPLSREQIGEIVEIQLHRVSDRLADRNIELR
                     TTPGAREWIANRGYDPVYGARPLKRLIQREVLDALAMKLLSGELHDGEVVTIDSSPGG
                     LVFQAQLTEAPLVA"
     gene            458..592
                     /locus_tag="VFI12_04185"
     CDS             458..592
                     /locus_tag="VFI12_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055636.1"
                     /translation="MDTIMGLVRAGITLAVIVIIIGLVAALFMIFFNLATGIDDRISE
                     "
     gene            691..2469
                     /locus_tag="VFI12_04190"
     CDS             691..2469
                     /locus_tag="VFI12_04190"
                     /inference="COORDINATES: protein motif:HMM:NF023260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3352 domain-containing protein"
                     /protein_id="HET7055637.1"
                     /translation="MYDRNQSPGQRVGGIERVLRALMALSLIAAIGAGLLVHSPARAQ
                     SSALLSTSKVTPADVVFYAELSLDTSSAQLQKFDELLTRLGSKESLVDAIKSTATSAN
                     SDVDLTGAEVSVAILPSALAEGASMSGDLVGAATGGQTTSELEKAASSAGNQGVVIVV
                     HPTDIATLKASMEKTAGTSAKTETYDGVEITTYTDENGDESAYAVSGDYLFVGQTVDD
                     IKAFVDAGKTGGKTLADLEGFQKTSDLLPAERVAFAFANGPALFEAATAAANNSAEVQ
                     SAQEMLPDFSGYTGLVVTADDPGIRFETVLVPEKGDGNAKAAGTAADLGMAERMPADT
                     AVFASGYDLGKSAVMNGLGLFLAVAFMGVTSGTSSSPEASPTPASIDDMYEELSRTFG
                     FNLKTGFLDQMTGPYGFGVWGLDSADPTQIDAALVTGVGDATVLGDTLGTVSLLVQAG
                     GQGRVNVTSKTVTGGSVNHVEFDASGSNMAVEYGVVGNEFVLGLNGGAETVMNGPADS
                     LATSDLYSTTLGYLPKEYQAVYFADIAQLAAAGESTGSSALGGDSAIADFLGTPTAGG
                     TVPQSFAAVTHVTDGYTHTSGILVVP"
BASE COUNT          540 a          867 c          960 g          525 t
ORIGIN      
        1 ggcccgagtt cctgaaccgc gtcgatgaac ttgtcatctt cgagccactg agccgcgagc
       61 aaatcggcga gatcgtcgag atccagctcc atcgggtttc cgaccggctg gcggatcgca
      121 acatcgagtt gcggacgaca cccggcgcgc gagagtggat cgccaaccgt gggtacgacc
      181 cggtatacgg ggcgcggcca ctcaagcgac taatccagcg cgaggtgctc gacgcgctgg
      241 ccatgaagct gctcagtggt gagttgcacg atggcgaagt cgtcacgatc gactcgtcgc
      301 ccggtgggct ggtcttccag gcgcagctca ccgaggctcc cctggtcgcg tagccaaggc
      361 gcatgaaaac gcggcagtcc tgtcagaaca gcgctgccgc gttgcgtttc ccggatataa
      421 tcggcggcag gtcgtcgtag cgccgggagg gtgcctgatg gacacaatca tgggcctggt
      481 gcgagcaggg attacactcg cagtgatcgt catcatcatc ggattggtgg cggcgctctt
      541 catgatcttc ttcaaccttg cgaccggaat cgacgatcgc atctccgagt agggattttt
      601 gcctcggcga tgcgtatacc gggatatgcg ttcaggagag gggccggctt tcggagtcgg
      661 cccgcttttt tcaccaagga ggcgaaccgg atgtacgatc ggaatcagag cccaggccag
      721 cgcgttggcg gaattgagcg cgtcttgcgc gcactcatgg cgctatcgct gatcgcggct
      781 atcggcgcag gactcctggt ccattcgccg gcgcgggcgc agtccagcgc attgctatcg
      841 acgtcaaagg tcacaccggc cgacgtcgtc ttctacgctg aactcagcct cgacacttcc
      901 agcgcgcaac tccagaaatt tgacgagctg ctgacccggc tgggctcgaa ggaatccctc
      961 gtcgacgcga tcaagagcac cgcgaccagc gcgaatagcg acgttgatct gaccggtgcg
     1021 gaagtctcgg tcgcgatcct gccgagcgcg ctcgccgagg gcgcgagcat gtccggcgac
     1081 ctggtcggtg cggcaactgg cgggcaaacc acgagcgagc tcgagaaggc agccagttcg
     1141 gccggcaatc agggcgtggt catcgtcgtt cacccgaccg atatcgccac gctcaaggcg
     1201 tcgatggaaa agacggccgg cacatccgcc aagactgaga cctacgatgg cgtcgagatc
     1261 acgacctata ccgatgagaa tggcgacgag agcgcgtatg ccgtttccgg cgattacctc
     1321 ttcgttggcc aaaccgtcga tgacatcaag gccttcgttg atgccggcaa gacgggcggc
     1381 aagacgctcg ccgacctcga gggattccaa aagaccagcg atcttctccc ggccgagcga
     1441 gtggcgttcg cgtttgcgaa tggacctgcc ttgttcgagg cggccacggc ggcagccaac
     1501 aattccgccg aagttcagtc cgcgcaggaa atgctccccg acttttcggg gtacaccggc
     1561 ctggttgtga cggccgacga cccgggtatc cgcttcgaga cggtgctggt gccggagaag
     1621 ggtgacggca atgccaaggc agcggggaca gcggctgacc tgggcatggc cgagcggatg
     1681 ccggccgata ctgccgtttt cgccagcggg tacgaccttg gcaagagtgc ggtcatgaac
     1741 ggactcggtc tcttcctcgc cgtcgccttc atgggtgtca cgagcggaac gtcttcatcg
     1801 ccggaggcca gcccgacccc ggcctcaatc gacgatatgt acgaggagtt gtcgcgaacc
     1861 tttggcttca acctgaagac cggcttcctc gatcagatga ccggaccata cgggttcggt
     1921 gtctgggggc tggattcggc cgacccgacg cagatcgatg cggcgctggt gaccggtgtt
     1981 ggcgatgcga ccgtgctggg cgacacgctc gggacggtgt cgctgctcgt ccaggccggt
     2041 gggcagggcc gggtcaatgt gacttccaag acggtcaccg gcggatcggt caaccacgtc
     2101 gaattcgatg cgtcgggctc gaacatggcg gtcgagtatg gcgtagtcgg caacgagttc
     2161 gtcctgggcc ttaatggcgg cgccgagacc gtcatgaatg gtcccgcgga ttcgctcgct
     2221 acgtccgacc tgtacagcac gacacttggg tatctgccga aggagtatca agcggtctac
     2281 ttcgcggata tcgctcaact ggccgcggca ggcgagagca cgggcagctc ggccctgggc
     2341 ggagacagcg cgatcgctga cttccttggc acgccgacgg cgggtggcac ggtgccgcag
     2401 tcgttcgcgg cggtcacgca cgtaaccgac gggtacactc acaccagcgg gatccttgtc
     2461 gtcccgtagt tcaaccgaac tcaaacataa cgccgcttct cctgaccgga gaggcggcgt
     2521 tgtcgtgcct gagccatccg cgatccggaa cgatcaaggc gcattgttcg ttgaagcagg
     2581 gtgagaccgg cggttccttg cgggaacctg ggccgggggt tcatttgcgg ggcatgaatg
     2641 tccatggtag cgttcagacc atgaaggacc tcgaccgcag gcattgctcg tggcacaccg
     2701 gcgtttcgcg tcggtaggcc ggagtcccat gcggaatcca cccccgagta gataatctcc
     2761 cggtgtgcaa tccgggatga ggagacaggg agcatggcga aggaacggaa cgaactggcc
     2821 gagcttgagc gcgcctggcg tcgctcggcg ccgaagggct cgcgccggga gatgctgaag
     2881 tggtcggcat tc
//
LOCUS       DASSGX010000242         3364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_589935, whole genome shotgun sequence.
ACCESSION   DASSGX010000242 DASSGX010000000
VERSION     DASSGX010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589935"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..1380)
                     /locus_tag="VFI12_04195"
     CDS             complement(112..1380)
                     /locus_tag="VFI12_04195"
                     /inference="COORDINATES: protein motif:HMM:NF024919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET7055638.1"
                     /translation="MAGRDQQRRGLAGSDHYLDMVDLYAARAARAKTVVAHDTKPLVV
                     LPIGRGERFPARAVRTSAYGFRSRRVRLASAVTAILIVFSLLAGTALADQQYRVREGD
                     TITIVGERFGVDPVDILKASGLDNPEYLYPGQELTIPGIADGDIGTGWVLGTYDVEYG
                     DTIGTIAWNLHVSASDLLAINGLAADDIIVPGQVLVVPDTPATKEETLAQLDELSSEQ
                     VTGESEEGAAQNAADGAATAGLPSSAWVSVPTYVQRRNLSCEYASTYIATAAFGPGID
                     ESVFWNSIPVTLNPHYGYRGDIDGWWGNYTDYGIYPEPLVPVLNANGFGGDAFYGDSD
                     PTQLMQQLAWGRPVVVWLAMWGDTGIRFEDEGSYTIFSGEHVMTAYGYDETGVYLSDP
                     ASGTSKFYDWSTFLWMWGTSDGMSLAVYPL"
     gene            1667..2875
                     /locus_tag="VFI12_04200"
     CDS             1667..2875
                     /locus_tag="VFI12_04200"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HET7055639.1"
                     /translation="MSYQVNEQLMERLAARAEAFGRSPWTFIDQYLNLHPDLIYFGNG
                     APAAELYPVEQLRRGAAEAWAGIGPTELDYGELQGYRPLRELIASRMAAQGIPVDPGQ
                     ILLTFGSQQGIDIAARLMLDKGDALAAEGPTYIGAMQAFDAYEPEYRLYPIDQAGFQV
                     DALIADVEATGRAPKIIYVIPNFQNPTGQTMSEERRAKLVAFAEEIGALIVEDDPYGE
                     FWYDAPPPPALRTRSDRVAYLGTFSKTIAPGLRAGWMVVPDELMARALMAKESSEVCG
                     VRATTRVVYETARGFLDEHVAEGRAFYAGRRDTILAALADSMPAGVTWSSPGGGFFVW
                     ITLPDGLTASAALPVAAEHGVGFLPGRFFYPAADGDDRSLRLSFSSLPEERIREGIAR
                     LGAAVDRLLA"
     gene            2900..>3364
                     /locus_tag="VFI12_04205"
     CDS             2900..>3364
                     /locus_tag="VFI12_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055640.1"
                     /translation="MNLDEVKDRLHTDNHQLWLLIGLCLAVLATMEAVQEAVEGAWPH
                     QRRVSRMDVGERRAHPVWALVALMILPAAILAVFNVGMMVWKNVPRTDEMTTGGALLG
                     IGWVLFLLASVDALRMRRLIARAGPVFPIALVVVLAAAIALLLSAFLDVCPSM"
BASE COUNT          540 a         1083 c         1128 g          613 t
ORIGIN      
        1 ccaccgcctc gctgcatgcg cgtccgcctt tttcaggacg caagcatacg ttcccggcgg
       61 caacgaaaca gcctgcccgg ccgggcaggc tgtcgctgga gaaatgccgc gctacaacgg
      121 gtaaaccgcg aggctcatgc cgtcgctcgt accccacatc cagaggaagg tcgaccagtc
      181 gtagaacttc gatgtgcccg aggccgggtc ggagaggtag acccccgtct cgtcgtatcc
      241 gtaggcggtc atgacatgct cgccggaaaa gatggtgtaa ctgccctcgt cctcaaaacg
      301 gatgccggta tcgccccaca ttgccagcca gacgaccacc gggcggcccc aggcgagctg
      361 ctgcatgagt tgcgtgggat cggaatcacc gtagaaggca tcgccgccga acccgttcgc
      421 attcaggacc ggtacgagcg gctccgggta gatgccgtag tcggtgtagt tgccccacca
      481 gccgtcgatg tcgccgcggt acccgtagtg cgggttgagc gtgaccggga tgctgttcca
      541 gaacaccgac tcgtcaatgc ccggaccgaa ggcggcggtc gcgatgtagg tcgaggcgta
      601 ttcgcagctt aaattccggc gctgtacata ggtcggcacc gaaacccagg cggaggaggg
      661 cagcccggcg gtggcggcgc cgtctgcggc attttgggcc gcgccttcct cgctctcgcc
      721 ggtcacctgc tcgctgctca actcgtcgag ctgcgcgagc gtctcctcct tggtggccgg
      781 ggtgtccggg acgaccaaaa cctgaccggg aacgatgatg tcgtcggcgg cgaggccgtt
      841 tatggcgagc aggtccgatg cgctgacgtg caggttccac gcaatcgtgc cgatcgtgtc
      901 cccgtattcg acgtcatatg tgcccaggac ccagccggtg ccgatgtcgc cgtccgcgat
      961 ccccgggatc gtcagctcct ggccgggata gaggtattcc ggattgtcga ggccactcgc
     1021 cttgaggatg tcgaccgggt caacgccgaa ccgctcgccg acgatcgtga tcgtgtcgcc
     1081 ttcgcgaacg cggtattgct ggtccgccag cgcggtcccg gcgaggaggg agaagacgat
     1141 caggatcgcg gtcacggccg aagcgaggcg tacccggcgc gagcggaagc cgtaggccga
     1201 ggttcggact gcccgggccg ggaatcgttc gccgcgaccg atcggcagca ccaccaacgg
     1261 cttggtgtcg tgcgcgacaa cggttttggc gcgcgcggcc cgtgcggcat agaggtccac
     1321 catgtccaga tagtggtcgc tccccgcgag cccacgtctc tgctgatccc gacctgccat
     1381 gcccgacgac ctcccctgac tgaacactga cggcgcgggg agtttgtgta agtacgggca
     1441 ggttcatcct acgacaaggt gtacgtacag gcaagtcgtt gtgtgccgtg gagtcaccat
     1501 tacgggacga aagagtctcg ctccacccaa aaacggatgt ccgtttccgg gcggaatcgt
     1561 gtctgtccgg cacgattggt ccggggtggt gggcgacaag aatcgattcc cggcgagatg
     1621 tcatactctt gcggttattt gcgaatcacc ggttccggag tcgggtgtgt catatcaggt
     1681 aaacgaacag ttgatggagc ggttggcggc gcgggccgaa gcgttcgggc gctcgccgtg
     1741 gaccttcatc gatcagtatc tgaatctcca cccggacctg atttacttcg gcaatggtgc
     1801 cccggcggcc gagctctacc cggtcgagca gttgcggcgc ggcgcggcgg aggcgtgggc
     1861 cgggatcggg ccgaccgagc tcgattacgg cgagctgcag ggctaccggc cgctgcgcga
     1921 gctgattgcc agtcgcatgg ccgcgcaggg catcccggtc gatccggggc agattctgct
     1981 cacgttcggc agccagcagg ggatcgatat cgccgccagg ctgatgctgg acaagggcga
     2041 tgcgctcgcg gctgaagggc cgacctatat cggggccatg caggccttcg atgcctacga
     2101 acccgagtac cggctctacc caatcgatca ggccggattc caggtcgacg ccctgattgc
     2161 cgatgtcgag gccaccgggc gtgccccgaa gatcatctac gtcatcccga atttccagaa
     2221 cccgaccggt cagacgatgt ccgaggagcg tcgcgcgaag ctggtcgcct tcgcggagga
     2281 gatcggcgcg ctgatcgtcg aggacgaccc gtatggcgag ttctggtacg acgcgccacc
     2341 gccgccggcc ctgcgcaccc gttcggaccg cgttgcctat ctcggcacct tctcaaagac
     2401 gatcgccccg ggcctccgcg ccggctggat ggtcgtgccg gacgagttga tggcgcgcgc
     2461 gctgatggcc aaggagtcgt ccgaggtctg cggcgtccgc gcgaccaccc gcgtcgtcta
     2521 cgagacggcc cgtggcttcc tggacgaaca cgtcgctgag ggtcgtgcct tctacgccgg
     2581 ccggcgtgac acgatcctgg ccgcgctggc tgattctatg ccggccggcg tcacctggag
     2641 ttcgcccggt ggcggcttct tcgtctggat caccctgccg gacgggctga cggcaagcgc
     2701 cgcgctgccg gtcgcggccg agcatggcgt aggctttctg cccggtcgct tcttctatcc
     2761 ggcggcagat ggcgacgatc ggtccctgcg cctgagcttc tcgtcgctcc ccgaggagcg
     2821 catccgggaa gggattgccc gcttgggagc ggccgttgat cgcctgcttg cgtaatccag
     2881 actccgccat ggtgagccga tgaacctgga tgaagtgaag gatcggctcc ataccgacaa
     2941 ccatcagctc tggctgctga ttggcctctg cctcgccgtg ctcgcgacca tggaagcagt
     3001 gcaggaggcc gtcgaaggcg catggccgca ccaacgacgc gtgagccgga tggatgtcgg
     3061 cgaacggcgg gcacacccgg tctgggcatt ggtcgccctg atgatcctgc cggccgccat
     3121 cctggccgtc ttcaacgtcg gcatgatggt ctggaaaaat gttccccgga ccgacgagat
     3181 gaccaccggc ggcgcgttgc tcgggatcgg ctgggtcctg ttcctgctcg ccagcgtcga
     3241 tgcgctccgc atgcggcggc tgatcgctcg ggctggcccg gtcttcccga tcgcgctcgt
     3301 ggtcgtgctg gcggcggcga tcgccctgct cctctcggcc ttcctcgacg tgtgtccgtc
     3361 gatg
//
LOCUS       DASSGX010000243         2190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1461715, whole genome shotgun sequence.
ACCESSION   DASSGX010000243 DASSGX010000000
VERSION     DASSGX010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1461715"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="VFI12_04210"
     CDS             complement(<1..762)
                     /locus_tag="VFI12_04210"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HET7055641.1"
                     /translation="MPTQTPFLNYADFDRLWDAFHSGPDAPTGQLFMGVRTTGIYCRP
                     GCPARQPKRTNVRFFATASAAERSGFRACKRCHPNEIQPNPKLAVVESACRYIDEHYE
                     DDVRLDALADALGVSTQTLQRTFREVIGITPRVYARERRLDQFRDELRNGSDVSRAIY
                     GAGFSSPSRVYERAGDDLGMTPAQYRRGAPQEMIQVALGPSPFGVVGVGRTGAGICSI
                     RLGNSPDDVLAAITAEFPHAEIALVEADPDLERIVQ"
     gene            925..>2190
                     /locus_tag="VFI12_04215"
     CDS             925..>2190
                     /locus_tag="VFI12_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055642.1"
                     /translation="MNRIRLDSLANRVQSASRRIRPSSEDAAATSEWVLRRGDQRYAG
                     TFDVIGNFPGFEEFAGLIVNAAITVTDRFLIVDEGAPHGFALGIGFLVDVSADPDPDT
                     FGDVLIRYRADEANVIFRLRPSRSRLALRSRGKPEDLVAALSDAGADVADLARDVADT
                     LRLSWDSVQSLETEAVIWRGQATAPLRPGLECAPASVWVTSTALVWGSSKGRGVNRLP
                     ISTIMRLSATSLHDEAGSPTVYVRSRVISDVRLDLPFIFNLAPVRDGMTARSEFLGLF
                     RPDAVIEGSVSARPQPWLEETPVASGDEEDDVIVLPGSETEGEEPDTESADDAGEPNT
                     EAADSEPEFETWANLVKPTMFPYVPPAEGGRATHRLAVGGADRAEPQYDPTGTRLSDA
                     IASWPGASSHDHAPAEPESAAITEPDLIPA"
BASE COUNT          343 a          727 c          744 g          376 t
ORIGIN      
        1 ctggacgatc cgttcgagat cgggatcggc ctcgaccagc gcgatttcgg cgtgcgggaa
       61 ctcagccgtg atcgccgcga ggacgtcgtc cggcgaattg ccgaggcgaa tcgagcagat
      121 accggccccg gtccgcccga caccgaccac gccgaacgga ctcgggccaa gcgcgacctg
      181 gatcatctct tgaggcgctc cccgccggta ttgggcgggc gtcatcccga ggtcgtcgcc
      241 ggcccgctca tagacgcggc tcggcgagct gaacccagcg ccgtagatcg cccggctgac
      301 gtctgatccg tttcgcagtt cgtcgcggaa ctggtcgagg cggcgctcgc gcgcgtagac
      361 acgcggcgtg atcccgatca cctcgcggaa ggtgcgttgc agggtctggg tgctgacacc
      421 gagggcatcg gccagcgcgt cgagccggac gtcatcctcg taatgctcgt caatgtagcg
      481 gcaggccgat tcgacgaccg cgagcttcgg gttcggctgg atctcgttcg ggtgacagcg
      541 cttgcaggca cggaatccgg atcgctctgc ggccgacgcg gttgcgaaaa agcgcacgtt
      601 ggttcgcttc ggctgccgcg ccgggcagcc gggtcggcaa tagatcccgg tggtgcgcac
      661 gcccatgaag agctggccgg tcggcgcgtc gggaccactg tgaaaggcgt cccacaggcg
      721 gtcgaagtcg gcgtagttga ggaatggagt ctgggtcggc atggctttca tctcccggtg
      781 aatcgctcct gacacgattc tcgggtcaat cccgggcgaa tactatccgc tttggctcac
      841 cgcatcccga attgcgttga tcaggcatcg cggctggggt agcatcgcgc ctgtgagcag
      901 tttccccggc aggtccgatc catcatgaac cgcattcgcc tcgattcgct ggccaaccgg
      961 gtccaatccg ccagtcgccg catccgcccg tcctccgagg acgccgcagc gacgtccgag
     1021 tgggtcttgc gacgcggtga tcagcgctat gccggcacgt tcgacgtgat cggtaacttc
     1081 cccgggttcg aggagttcgc cggcctgatc gtcaatgccg caattaccgt aacagaccgg
     1141 ttcctgatcg tggatgaggg cgcgccacac gggttcgcgc tcggcatcgg cttcctcgtc
     1201 gacgtctcgg ccgatcccga tccggacacg ttcggcgatg tcctgatccg ctatcgggcc
     1261 gacgaggcga acgtgatctt ccggttgcgg ccgagccgat cgcgcctggc attgcgctcg
     1321 cggggaaagc ccgaggatct ggtcgcggcg ctgagcgatg ccggcgctga cgtagccgat
     1381 ctggcccggg acgtggcgga caccctgcgg ctctcgtggg actcggtcca gagcctcgag
     1441 acggaggcag tcatctggcg cggccaggcg acggccccgc tgcggccggg cctggaatgc
     1501 gcgccggcca gcgtctgggt cacgtcgaca gcactggtct ggggcagctc gaagggcagg
     1561 ggcgtcaacc ggctgccgat ctcgacgatc atgcgtctct cggccaccag cctccacgat
     1621 gaggccggtt cgccaaccgt ctatgtccgc tcccgggtca tctcggatgt ccgcctcgac
     1681 ctgccgttca tcttcaacct cgccccggtg cgggatggga tgacggcccg ctctgaattt
     1741 ctcgggctgt ttcggcccga cgcggtgatt gagggcagcg tctccgcgcg accgcagccc
     1801 tggctggagg agacgccggt tgcatcgggc gacgaggagg acgatgtcat cgtcctgcct
     1861 ggcagcgaga ccgagggcga agagccggac acggaatcgg ccgatgatgc cggggagccg
     1921 aacaccgaag cggcggattc ggaaccggag ttcgagacgt gggcgaatct cgtcaaacct
     1981 acgatgttcc catatgttcc cccggccgaa gggggccgtg ccacgcatcg cctcgccgtc
     2041 ggtggtgcgg atcgcgccga gccccagtac gaccccaccg ggacgcggct gtccgatgcg
     2101 atcgcgtcct ggccaggcgc ctcgtcgcat gaccatgcac ctgccgagcc cgaatcggcc
     2161 gcgatcacgg aaccagacct catcccggcc
//
LOCUS       DASSGX010000244         3201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_918436, whole genome shotgun sequence.
ACCESSION   DASSGX010000244 DASSGX010000000
VERSION     DASSGX010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_918436"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="VFI12_04220"
     CDS             complement(<1..937)
                     /locus_tag="VFI12_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HET7055643.1"
                     /translation="MSAVNRPVANLNLPSVTKRAPWQDRPETRISNWMAPWDFDEPID
                     AGLIGAPYSGASISASGAAGGPEAVRMAFRFNTTYTPDWDTDIKDLTVRDLGDISGHM
                     TDVSVAHGNIENAVRDALTVADPFLPIIVGGDHSITAPAVRGFAAANEGKKIGIINID
                     AHYDVRVLDSGPHNGTPFRQLIEGGVVSGKNVVELGIHGFMNAAYYRQWCNEQGVTTI
                     TGRDVHKRGMETCILEALGIAGDGTDLIYLSVDIDCLAYPWAPGTSAASAEGLSAWDV
                     LEAMYICGQHSNVAALDVVEIDPTKDVHDLTARSGC"
     gene            complement(938..1612)
                     /locus_tag="VFI12_04225"
     CDS             complement(938..1612)
                     /locus_tag="VFI12_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HET7055644.1"
                     /translation="MCQGLTGPTHDGQDEWFLDSPALRSFIGAVQRVRAAESDPARVV
                     ALVEPAFTELLADQTWLPDHFAEPSPESGMGSGIGMWLLYRAGDGNLALSSLVVPPGA
                     ATPVHDHLAWGLVGLYRGTQDEVVFTRTDDASVEDKATLETMVQRSLAPGDLYEILPE
                     TDIHKVRTTSDVTSVSLHLLGNDNGCKMRHRFHPDEGRVEPFKSGWLNVPCTEPAAAG
                     SSQGMG"
     gene            complement(1618..2319)
                     /locus_tag="VFI12_04230"
     CDS             complement(1618..2319)
                     /locus_tag="VFI12_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7055645.1"
                     /translation="MLALRDVHTYYGRIQALKGISIEVNQGEVVTLIGSNGAGKSTTL
                     KTISGLLTPASGEVSLNGQRLNGMPAHKIVEKGVVQSPEGRRVFPRMTVLENLEMGAF
                     SKRSVDQADLARVFELFPRLKERVSQKAGTLSGGEQQMLAIGRALMAHPALLLLDEPS
                     MGLSPILIEAIFEIIKEINAQGVTVLLVEQNALMALAVAHRGYVMQTGSIVLSDNAAA
                     LMQNDMVRKAYLGEE"
     gene            complement(2343..>3201)
                     /locus_tag="VFI12_04235"
     CDS             complement(2343..>3201)
                     /locus_tag="VFI12_04235"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7055646.1"
                     /translation="SVDLRIPRGAIQGLIGPNGAGKTTFFNIIAGIYQLTEGEILFEG
                     KPIQRSNVGAFSSAGLRPDQIASLGISRTFQNIRLFANMTALENVLIGMHPRLKSSPL
                     GALLKLSSVKREERQARQRGRELLAYVGLAGMGEEIAKNLPYGDQRRLEIARALASDP
                     HLLLLDEPTAGMNPNETGQMTRFIDQIRRDFTLTILLIEHDMKVVMGISDTVAVLDRG
                     QKIADGAPRDVQHNPRVIEAYLGKAGAAAAASAIGSREGEAIAETPPEGPGGDLGTGI
                     VRPGAGGGR"
BASE COUNT          539 a         1052 c         1011 g          599 t
ORIGIN      
        1 agcagcccga gcgcgcggtc aggtcatgga cgtctttggt cgggtcgatc tcgacgacat
       61 ccagcgccgc cacgttcgag tgctgcccgc agatgtacat cgcctcgagc acatcccatg
      121 cactcagtcc ctcggcgctg gccgcgctcg tgcccggcgc ccatgggtac gcgaggcagt
      181 cgatgtcgac gctgaggtag atcagatccg tgccgtcacc ggcgatgccg agcgcctcaa
      241 ggatgcaggt ctccatcccc cgcttgtgca cgtcgcggcc ggtgatcgtg gtcacgccct
      301 gctcattgca ccactgccgg taataggcgg cgttcatgaa gccgtggatg ccgagctcga
      361 cgacattctt gccgctgacg acaccacctt cgatcaactg gcggaagggg gtgccgttgt
      421 ggggaccaga atccagcacc cgaacgtcgt agtgggcatc gatattgatg atgccgatct
      481 tcttgccctc gttggcggcg gcgaagccgc gcacagccgg agcggtgatc gaatgatctc
      541 caccgacgat gatcgggagg aacggatcgg cgacggtcag cgcatcccgg accgcattct
      601 cgatgttgcc gtgggccacg ctcacgtcag tcatgtgacc gctgatatcg ccgaggtcgc
      661 ggacggtcag atccttgatg tcggtgtccc agtccggcgt ataggtcgta ttgaagcgga
      721 acgccatccg caccgcttcg ggaccgcccg ccgcgcccga cgccgaaatg gacgcccccg
      781 agtagggagc accgatcagc ccggcatcga tcggctcgtc gaagtcccag ggggccatcc
      841 agttgctgat gcgggtttcc ggccgatcct gccatggcgc gcgcttggta acggacggca
      901 ggttgagatt ggcaactggt cgattgactg cagacattta ccccattccc tgcgagctgc
      961 ccgctgcggc cggctccgtg cacggtacgt tcagccagcc agatttgaac ggctccacgc
     1021 gtccctcgtc ggggtggaac cggtgccgca tcttgcagcc attgtcattt ccgagcaagt
     1081 gcagggacac agacgtcaca tcggaggttg tccggacctt gtggatgtcg gtttccggga
     1141 ggatctcgta gaggtcgccg ggcgccagcg agcgctggac catcgtttcg agcgtcgcct
     1201 tgtcctcgac gctggcatca tccgtccggg tgaagacgac ttcatcttgc gtgccccggt
     1261 acagcccgac gagcccccag gccagatgat catggactgg agtcgccgca ccgggcggca
     1321 cgaccaggct cgacagggcc aggtttccgt cgcccgcgcg atagagcaac cacatgccga
     1381 tgccactgcc catgccgctc tcgggcgagg gctcggcaaa gtgatccggc agccaggtct
     1441 gatcggcgag cagctccgtg aacgccggct cgaccagcgc cacaacacgg gccggatcgg
     1501 attccgctgc ccggacgcgc tgaaccgctc cgataaacga ccgtagcgcg ggactatcga
     1561 gaaaccactc atcctgtccg tcgtgggtcg gaccggtcaa tccctggcac acgtcgctta
     1621 ctcctccccg agatacgcct tacgcaccat gtcattctgc atcaacgccg cggcgttgtc
     1681 gctgagcacg attgacccgg tctgcatgac gtagccgcgg tgcgcgacgg ccagggccat
     1741 gagcgcgttc tgctcgacga gcagaacggt gacgccttgc gcgttgatct ccttgatgat
     1801 ctcgaagata gcctcaatca ggatcggcga gagccccatc gacggctcgt cgaggagcaa
     1861 aagcgccggg tgcgccatca gtgcgcggcc gattgcgagc atctgttgct cgccgccaga
     1921 gagcgtgccc gccttctgac tgacgcgctc cttgaggcgg ggaaacagtt cgaacacccg
     1981 ggcgagatcc gcttggtcga ccgatcgctt cgagaatgcg cccatctcga gattctcgag
     2041 cacggtcatg cgcgggaaga cgcggcggcc ctcgggtgat tggacgacgc ccttctcgac
     2101 gatcttgtgc gcgggcattc cgttgaggcg ttggccgttg agcgacacct cgccgctcgc
     2161 gggagtcaag agaccggaaa ttgtcttgag cgtcgtgctt ttgcccgcgc cgttcgaacc
     2221 gatcagtgtg acgacttcgc cctggttgac ctcgatagag atccccttga gcgcctgaat
     2281 ccgtccgtag tacgtatgga cgtcgcgaag cgcgagcatc ggcgcgccgt tctggaccga
     2341 cattatcggc cccctccggc gcccggccgg acgatgccgg tccccaggtc cccgcctggt
     2401 ccctccggcg gtgtctcggc gattgcttca ccctcccggc tgccgatggc ggacgcggcg
     2461 gcggcggcgc cggccttccc gagataggct tcgatgactc tcggattgtg ctgtacatcg
     2521 cgcggtgcgc cgtcggcgat tttctgaccg cggtccagca ctgccaccgt gtcggaaatg
     2581 cccatgacta ccttcatgtc gtgctcgatc agcagaatgg tcaaggtaaa gtcgcgccga
     2641 atctgatcga tgaagcgcgt catctggccg gtctcgttcg ggttcatgcc ggcggttggt
     2701 tcgtcgagca ggagcaggtg cggatcactg gccagcgccc gcgcgatctc cagccggcgc
     2761 tgatcgccat acgggagatt cttggcgatc tcctcaccca tcccggccag cccgacatac
     2821 gccaggagct ctcgcccgcg ctgccgagcc tgtcgctcct cacgcttgac ggacgacaat
     2881 ttgagcagcg cgcccagcgg actcgacttc agccgcgggt gcatgccgat cagcacgttt
     2941 tccagcgccg tcatattcgc gaacagccgg atgttctgga acgtccgcga gatgccgagc
     3001 gaggcaatct ggtccggtcg cagcccggcg ctgctgaacg cccccacgtt tgagcgctgg
     3061 atcggcttgc cctcaaagag gatctcaccc tcggtcaact ggtagatgcc cgcgatgatg
     3121 ttgaagaacg tggtcttgcc ggcgccgttt ggcccgatca ggccctggat cgcgccgcgc
     3181 ggaattcgca gatcgacgct g
//
LOCUS       DASSGX010000245         6136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1243106, whole genome shotgun sequence.
ACCESSION   DASSGX010000245 DASSGX010000000
VERSION     DASSGX010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6136
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243106"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..707
                     /locus_tag="VFI12_04240"
     CDS             66..707
                     /locus_tag="VFI12_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055647.1"
                     /translation="MHGVSDMILPDRLEAGCYAIGAVITQGEVTLQGVREPDMLPLTE
                     KLREAGAEVWHQEADMLVRTRRELVGVEIQTLPFPGFPTDLQAPFAVLMTQSAGLSRI
                     HERVFDDRLRYTDELLKMGADIRVEKFAPNRYGTKADIMGPTPLKGADVRALDIRAGA
                     GMVLAGLVAAGRTTISDVYHLDRGYEELVPKLRQIGAQITAIGYDSPADGTAD"
     gene            737..1207
                     /locus_tag="VFI12_04245"
     CDS             737..1207
                     /locus_tag="VFI12_04245"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7055648.1"
                     /translation="MDTELYVDRLALGERDDRSEFATHLMADQRTGQRVLIVDDEAAV
                     ADLIEAVLVQEGYTVAIARDGVQGILLARDWKPDLVLMDIMLPGLDGTTAIRRLKSDP
                     ATAELPIVAMSAGRTIRRQSNELADADAALAKPFDIDALLAQVEFLLSRRRAEP"
     gene            1268..1975
                     /locus_tag="VFI12_04250"
     CDS             1268..1975
                     /locus_tag="VFI12_04250"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HET7055649.1"
                     /translation="MSRSITVVIPTYNERANLEPILFELFTTIPDAHVVVVDDNSPDG
                     TGMLADEIARRDSRLTVVHRPRKEGIGPAYIAGFARALGCQTELIAAMDADGSHSPAD
                     LARMIGAAANADLVVGSRYVDGGQTVGWPRWRRSLSRFGGMYARWVLGAPISDLTAGF
                     KVYRRAALASLPLDRIRSDGYGFQIETTWYIWRSGLRVREVPITFHDRIAGKSKLSRR
                     IVIEAMVMVWRLRFSGR"
     gene            complement(1926..3083)
                     /locus_tag="VFI12_04255"
     CDS             complement(1926..3083)
                     /locus_tag="VFI12_04255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7055650.1"
                     /translation="MTLPGRRIALVHDYLTQFGGAEQVLAVLQRLFPEATTFTTLHDP
                     AADLPGIDQSRVVESRLGAVRWFRQHHRAALPLFPMAMRDLRRRLVDYDVIIADSSAW
                     AHQLKPRPDQAFIVYCHSPARFLYGDADYLGATGVEGPVARAFHSVSAPFRWLDRRAY
                     RRADVVLANSQQVAGRLAHRLGIDATVLHPPIDVSALRPDGFREPEDWYLVVSRLVPH
                     KRVDLVVETATAHHLPVKIVGVGRELDRLQARAGPTVQFLGFQPHAQVIEHFQRCRAF
                     ILPGVEDFGMTAVEAQAAGRPVLAFGRGGALESVQEGVSGVLFAEQTPDSLLAAMRRL
                     DALRVSPEACVQNARLFDESVFSAGMLEAVERARVISDQRSGAARPSPSLR"
     gene            3416..4981
                     /gene="rny"
                     /locus_tag="VFI12_04260"
     CDS             3416..4981
                     /gene="rny"
                     /locus_tag="VFI12_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HET7055651.1"
                     /translation="MDGLFVPVVEALLVAAVSIAGTLLYLERRANSRLREAQRQADQV
                     LQEAEEVRRDAEISAKDEAIRLRGDLDRELTTRRKEIERIEKRIEQKEENIDRKVSSV
                     EQRETALRRQEQSVGQLKETFEKDKATALQEIDDLRNRRLGELERIAQLTAEEARQEL
                     VAEVESEARAMAARRIHEIDQEIQAEADRRARKVLATTIQRIATDYIAESTVSVVPLP
                     SDEMKGRIIGREGRNIRALEQATGVDLIVDDTPEVITVSAYDPLRRELARRVLQKLLQ
                     DGRIHPTRIEDVVNKTQSELDTTIREEGERVAYEANVVGLHPDLIKLLGRLKFRTSYG
                     QNVLGHSLETSIIAGALAAEIGADINVCKTAGLLHDIGKAVDHEVEGPHALIGADIAR
                     RLGRSAKIVHAIAAHHGEEEPQTVEAFIVATSDAISGSRPGARREMVETYIKRLEALE
                     GVANSFEGVEKSFAIQAGREVRILVSPNKIDDLAASRLARDVSKKIEESLEYPGQIKV
                     TVIRETRAVDYAR"
     gene            5587..5838
                     /locus_tag="VFI12_04265"
     CDS             5587..5838
                     /locus_tag="VFI12_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003181955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HET7055652.1"
                     /translation="MKVSSKSRPSAVAGAIAGVVRELGRAEVQAIGAGATNQAIKAVA
                     IARDYLRETGIDAVCLPAFIDITIDNEDRTAIRLVIEPR"
     gene            5847..>6136
                     /locus_tag="VFI12_04270"
     CDS             5847..>6136
                     /locus_tag="VFI12_04270"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HET7055653.1"
                     /translation="MTLTGVADLHTHTTASDGLDAHATLVQRARDVGLAVIAIADHDT
                     VDAHLTLSDADTRGVSVIPAIELSANVGTAEVHILGYFVDPTSKRLADALAQL"
BASE COUNT         1163 a         2023 c         1919 g         1031 t
ORIGIN      
        1 accggatggg cgcgcaaatc acggggctcg gctcgccgac catcaccatc tagggtgttg
       61 atcgcctgca tggcgtctcg gacatgatcc tgcccgatcg gctcgaagcg ggctgctacg
      121 ccatcggcgc agtcatcacg cagggcgagg tcacgctgca gggcgtccgc gaacccgaca
      181 tgctcccgct gaccgaaaag ctgcgcgaag ctggggccga ggtctggcac caggaagccg
      241 acatgctcgt ccgcacgcgg cgcgagctgg tcggggttga gatccagacg ctccccttcc
      301 ccggcttccc gaccgacctg caggcgccat tcgccgtcct gatgacgcaa tccgccggcc
      361 tgtcgcgaat tcacgagcgc gtcttcgacg accggctccg gtataccgat gagctgctca
      421 agatgggagc ggacattcgg gtcgagaagt tcgcgccgaa ccggtacggc acaaaagcgg
      481 atatcatggg ccccacacct ctcaagggcg ccgatgtgcg cgcgctcgat atccgggcag
      541 gagcgggtat ggtattggcc ggcctggtcg ccgccgggcg gaccacgatt tctgacgttt
      601 atcatctcga tcgcgggtac gaggagctcg tgcccaagct gcgccagatc ggcgcgcaga
      661 tcaccgcgat cggttacgac tcaccggcgg atggcaccgc cgactaaggg ggacgggcat
      721 ggattcgatc gattccgtgg acactgagct ttatgtggac cggctggcgc tcggtgagcg
      781 agatgatcga tcggagttcg ccacccacct gatggcggac cagcgcacgg gtcagcgcgt
      841 gctaatcgtc gatgatgagg ccgcggtcgc ggacctgatc gaggcggtcc tcgtccagga
      901 gggctacacc gtcgccatag cccgcgacgg agtccagggc atcctccttg cgcgcgactg
      961 gaagcccgac ctggtcctga tggatatcat gctccccggc ctcgacggca cgactgcgat
     1021 ccggcgcctc aagagcgatc cggcgaccgc cgagctgccg atcgtagcca tgtcggccgg
     1081 ccgcacgatc cggcgccagt ccaacgagct ggcagacgca gatgccgcgc tggccaagcc
     1141 gttcgacatc gacgcgttgc ttgcccaggt cgaattcctg ctctcgcgtc gccgcgccga
     1201 gccgtaacgc tcctgaagcg gagattccag cccccattct gcgctcgact gtgttcggag
     1261 tgaatgcgtg agccgatcga tcacggtcgt cattccgacc tataacgagc gtgccaatct
     1321 cgagccaata ctgttcgaac tcttcacaac cataccggac gcccatgttg tcgtggtcga
     1381 cgacaattct ccggacggca ccggaatgct cgcggatgag atcgcaagac gcgattcgcg
     1441 cctgaccgtc gtccaccgcc cgcgcaagga gggcatcggc ccggcctaca tcgccggctt
     1501 cgcccgggcg ctgggctgtc agacggagtt gatcgcggcg atggacgcgg atggctccca
     1561 ttctcctgct gacctcgccc gtatgatcgg ggccgccgcc aacgcggatc tggttgtcgg
     1621 ctctcggtac gtcgatggtg gccaaacggt cggctggccg cgctggcgcc gttccctctc
     1681 gcgcttcggt ggcatgtatg cgcgctgggt gctcggcgcc ccgatctccg accttacagc
     1741 cggtttcaaa gtctaccggc gcgccgcact ggcctcattg ccgctcgatc gcatccgatc
     1801 cgacggctac ggcttccaga tcgagacgac ctggtacatc tggcgctcgg gtttgcgcgt
     1861 gcgggaagta ccgattacgt ttcacgatcg catcgccggc aagtcgaagc tttcgcgccg
     1921 catcgtcatc gaagcgatgg tgatggtctg gcggctccgc ttctctggtc gctgatcacc
     1981 cgggcacgct cgacggcttc cagcattccc gcgctaaata cagattcgtc gaacaggcgc
     2041 gcgttctgga cgcacgcttc cgggctcacc cgcaacgcgt ccagccgccg catcgccgcc
     2101 aggagcgaat ccggcgtctg ctcggcgaac aggacaccgc tgacgccctc ctggaccgat
     2161 tccagggcgc cgccacggcc gaaggcaagg acgggccgac cggctgcctg ggcctcaact
     2221 gccgtcatcc cgaaatcctc gaccccgggc aggatgaacg cgcggcatcg ctggaagtgc
     2281 tcgatcacct gcgcatgggg ctggaacccc agaaactgga cggtcgggcc ggcccttgcc
     2341 tgcaaacggt ccagttcccg gccgacgccg acgatcttga ccgggaggtg gtgcgccgtg
     2401 gccgtctcaa cgaccagatc gacgcgcttg tgcgggacca gacgggacac gacgaggtac
     2461 caatcttcgg gctcccggaa tccgtccggc cgcagcgcgg agacatcgat cggcggatgg
     2521 agcactgtcg cgtcgatccc gagccgatgg gcgagccggc ccgcgacttg ctgactgttc
     2581 gcgagcacaa catccgcacg ccggtacgcc cggcgatcga gccagcggaa gggcgcggag
     2641 accgagtgga aggcgcgcgc cacgggtccc tccactcccg tcgccccgag gtaatccgca
     2701 tcgccgtaga ggaacctggc cgggctgtgg caatagacga tgaatgcctg gtccggccgg
     2761 ggcttgagct ggtgcgccca tgcgctcgaa tccgcgatga tcacgtcgta atcgacgagg
     2821 cggcggcgca aatcccgcat cgccattgga aaaagcggga gcgctgcccg gtggtgctgc
     2881 cggaaccaac gcaccgcgcc gagccgactt tccacaaccc gactctgatc gatgcccggc
     2941 aggtcggcgg ccggatcatg cagcgtggtg aaggtcgtcg cttcgggaaa gagccgctgc
     3001 aggaccgcaa gcacctgctc ggccccgccg aactgcgtca gatagtcgtg gacgagcgcg
     3061 atccggcgac ccggcaatgt catgacccgg cgcctcgtgt ttgtcgcctg caaggcttat
     3121 tcattatgta atgtcctact gtgacgttgg cgacctgttc cgccgttatt tccttgcgta
     3181 tgcttgatag agccattggg ttgctccact gtcatgtact tacgccgatt cttgcaatat
     3241 gcaggtttat accgggtttg acagtatttt cgagaaaacc gtatgatcac aggaacatcg
     3301 cggcactcga cgtctgtcga atccccgata ttagacactg attgtttcgc atcaggcgaa
     3361 accgaaccaa ttggaaccgc gccactccat tctggcgccc caggagagta tgaccgtgga
     3421 cggactattt gtccccgtgg tcgaagcgct cctcgtcgct gcggtctcaa ttgcaggcac
     3481 attgctctac ctggaacgcc gcgccaactc gcgtctgcga gaggcccagc gtcaggctga
     3541 tcaggtcctg caagaggccg aagaagtccg acgagacgcc gagatctcgg ccaaggatga
     3601 agctattcgc ctccgtggcg atctggaccg tgaactgaca acccgccgca aagagatcga
     3661 gcggatcgaa aaacggatcg aacaaaaaga agagaacatc gaccgcaagg tcagttctgt
     3721 cgagcagcgc gagactgccc tccgccgcca ggagcaatca gtcggccagc ttaaggagac
     3781 cttcgaaaag gacaaggcca ccgcgcttca ggagattgac gatctccgca atcgccggct
     3841 cggcgagctc gagcgcatag cccagttgac cgccgaagag gcccgccagg agctggtggc
     3901 cgaagtcgaa tccgaagctc gcgcaatggc cgcgcgccgc atccacgaga tcgaccagga
     3961 aattcaggcc gaggccgacc gccgcgcccg gaaggttctc gcgaccacca ttcagcggat
     4021 cgccaccgac tacatcgcgg agtcgaccgt ttccgtcgtt cccctgccga gcgatgagat
     4081 gaaaggccgc atcatcggtc gcgagggccg caacatccgc gcgctcgagc aggccaccgg
     4141 tgtcgacctg atcgtcgacg atacgccaga agtgatcacc gtctcggcct acgatccact
     4201 ccgccgcgag ctggcccggc gggtgctgca aaagctgctg caggacggcc ggatccaccc
     4261 gacacgcatc gaggacgtcg tcaacaagac gcagtccgag ctcgacacca cgatccgcga
     4321 ggaaggcgag cgcgtcgcct acgaggcgaa cgtcgtcggc ctccacccgg acctgatcaa
     4381 gctacttggc cgcctcaagt tccgcaccag ctacgggcag aacgtgcttg gccactcgtt
     4441 ggagacgtcg atcatcgccg gcgcgctcgc cgccgagatc ggcgcggata tcaacgtctg
     4501 caagacggcc ggcctgctcc acgacatcgg caaggcggtc gaccacgagg tcgaggggcc
     4561 gcacgcgctg atcggcgccg acatcgcccg tcgcctcggt cggtcggcca agatcgtcca
     4621 cgcgattgcc gcccaccacg gcgaagaaga gccgcaaacg gtcgaggcgt tcatcgtggc
     4681 gacctccgac gcaatcagcg gttctcgtcc gggcgcgcgc cgggagatgg tcgagacgta
     4741 catcaagcgg ctcgaggcgc tcgaaggcgt cgccaactca ttcgagggcg tcgagaagtc
     4801 cttcgcgatc caggccggcc gcgaggtccg catcctggta tcaccgaaca agatcgacga
     4861 ccttgccgcg agccgcttgg cgcgcgacgt gagcaagaaa atcgaggagt cgctcgagta
     4921 tccgggccag atcaaggtca cggttatccg ggagacccgg gcagtcgact acgctcggta
     4981 gcattgaccg cttaatgccg ttcatgtagc cttctgccag tgcattccaa accactcgcc
     5041 gacgagtgcc cgaattctca tattgcctac cgatcgccga gcttgaacga tcgaatccga
     5101 tattcaagct cggtgtccat gacaacggag tcctcgatcg gggagcaggt tccagggttg
     5161 gcttgaacgg agtgatcggc gtcgaacgtt gatcgagagt ggctgcaccg agttggcgcc
     5221 caccgtagcg tgcgccaacc acggacgacc ggcgattggt cggtagttcg cagagatcga
     5281 cgcgtgacct ggcgcaaatg accttgcgca tccgcctgga gcacctggat ggatcgattc
     5341 ttcagcaaac tcgacttgcg cgacatcaag attgacccgg aacggtcaac attctccccg
     5401 gacgacgaat tcgaatacgg cggcgaacca gattcgagcc cgaacggcgc atcggcagtg
     5461 ctggacgaca ctccagctgt cgagccctcg gcggacgcat gggttgatga agcgccggta
     5521 gcccccgaga ccaaccactc ggacggctat gaaccaacgg tcgcctctaa cggtgggacc
     5581 gacgtcctga aggtctcgtc caagtcccgg ccgagcgcgg tcgccggagc gatcgcggga
     5641 gtggtccgcg agctcggaag ggccgaagtc caggcgatcg gcgcgggcgc gacgaatcag
     5701 gcaatcaagg ccgtcgccat cgcgcgtgac tacctgcgtg agaccggtat cgatgccgtc
     5761 tgccttccgg cgttcatcga catcacgatc gacaacgagg atcgaaccgc gatccggctg
     5821 gtcatcgagc cgcgatagct cgacgcgtga cgctcaccgg ggtcgccgac cttcataccc
     5881 acaccaccgc atccgatggc ctcgacgcgc acgccacgct tgtgcagcgg gcgcgcgacg
     5941 ttggcctcgc cgtcatagcg attgccgatc acgatacggt cgacgcgcac ctgaccctct
     6001 ccgatgcaga tacccgcggc gtatcggtca ttccggcaat cgagttgagc gccaatgtgg
     6061 gcacggctga ggtccacatc ctcggctact tcgtcgatcc gacgagcaag cgcctggccg
     6121 atgcgctcgc acagct
//
LOCUS       DASSGX010000246         2410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1243131, whole genome shotgun sequence.
ACCESSION   DASSGX010000246 DASSGX010000000
VERSION     DASSGX010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243131"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1115
                     /locus_tag="VFI12_04275"
     CDS             <1..1115
                     /locus_tag="VFI12_04275"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET7055654.1"
                     /translation="MPHSDGWPGQPAAGGLRDGSGRAVAGEPIPATVSGPDLYAISHD
                     LKNRLTLIKGWADLLQRRVAAGLDRPAILQGLATIRSSAEAMDELLGELLYAGRVELG
                     HRQHLPRADVDLWELTERLVEQHRPLAQSHALTVTGPGAGQVIGNWDAGSIERILANL
                     LSNAIKYSPSSGQIMITIEQGGQEARLAVHDHGIGIPRPALPHIFEPFYRAGNVADQH
                     DSPSVSGIGIGLFAARKLANQHDGRIEVTSTERQGSTFTLVLPLRGYPRAARDGAGQP
                     RSHDAMKERSPMPEDVLPDDLAQSSEGSPQPEPAAEADETDARWQVMPRNTGDDPAID
                     VVDFQSMQSFPASDPPAWPRRYSDLAGKQPNRPDST"
     gene            complement(1237..1929)
                     /locus_tag="VFI12_04280"
     CDS             complement(1237..1929)
                     /locus_tag="VFI12_04280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7055655.1"
                     /translation="MKILLVDDATEVVEAVRLGMMLQWREVEILDAGSGDAALDLVER
                     EAPDLVLLDIGLPGMDGYEVLREIRAFSDVPVIMLTAQDTTMSKIKGLELGADDYITK
                     PFDHLELLARIKAVLRRLDMSAPKIRAPSFQSGDLAMDFSAQEVHLNGVRLELTPTEY
                     KLLYHLVRNAGQTLTHGTLLARVWGREYVNEVDYVRVYIRRLRSKLGDDPERPRYIQT
                     ERGLGYRFLRPK"
     gene            complement(1926..>2410)
                     /locus_tag="VFI12_04285"
     CDS             complement(1926..>2410)
                     /locus_tag="VFI12_04285"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7055656.1"
                     /translation="PQRFDARALTRDVHSSIASLMEARDQVLKLTVPADPVWIYADRG
                     RLEQVLLNLLSNATKFSPDGASILLALRADAEGTTWSVTDNGRGIGPEDQVHLFERFF
                     TREAGEVGRNPGAGLGLPIALAIAQAHGGTIVVDSAVGRGSTFTVAIPRRLTLEADEE
                     "
BASE COUNT          449 a          791 c          771 g          399 t
ORIGIN      
        1 cgatgccgca cagcgacggc tggccgggcc agccggctgc cgggggactg cgcgacggca
       61 gcgggcgagc tgtcgccggc gagccaatcc cggcgacggt ctcagggccg gacctgtacg
      121 cgatctcaca tgacctgaag aaccggctca cgctcatcaa aggctgggcc gacctgctac
      181 agcgacgagt cgcagcgggg ctcgatcgcc ccgcgatcct tcaggggctc gcgaccatcc
      241 gctcatccgc cgaagctatg gatgagttgc tcggcgagtt gctctacgcg gggcgggtcg
      301 agttgggcca ccggcaacac ctgccgcgtg ccgatgtcga tctctgggag ctcaccgaac
      361 ggctggtcga gcagcaccgc ccgctggcac agagccatgc gctcacggtc accggcccgg
      421 gtgccggcca ggtgatcggc aactgggatg ctggcagcat cgagcgcatc ttggccaacc
      481 tattgagcaa tgcgataaag tacagccctt ccagtgggca aatcatgatc accatcgagc
      541 agggcggtca ggaggcccgt ctggcggtgc acgaccatgg tatcggcatc cctcggccgg
      601 cgctcccgca catattcgag ccgttctacc gcgccgggaa cgtcgccgat cagcacgact
      661 caccgagcgt gtcggggatc gggatcgggc tcttcgccgc tcggaaactc gccaaccagc
      721 atgacggccg catcgaggtc accagcaccg agcgacaggg aagcacattc acactggtat
      781 tgccgttgcg gggctatccg cgggcagcac gcgacggggc aggacagcct cgatcgcacg
      841 acgcgatgaa ggagcggtcc ccaatgccgg aagatgtctt gccggacgat ctagcgcaat
      901 ccagcgaagg ttcgccccag ccagagccgg cggctgaggc cgatgagacc gacgctcggt
      961 ggcaggtcat gccacgcaac accggcgacg atcccgcgat tgacgttgtc gactttcagt
     1021 cgatgcaatc attcccggcc agtgatcccc ccgcgtggcc gcgccggtat tccgacctag
     1081 cggggaaaca gccgaacagg ccggacagca cataaccgtc aggtcggcct caccgcggcg
     1141 cgatcgctga gatctgacgc gcgcaggtca ggcagagagc ggacatgacg atcggtcgct
     1201 cgccctggcg caccactgga ggggcatggc gcctgctcat ttcggacgga gaaaccgata
     1261 gcccaggcca cgctcggtct ggatgtagcg gggccgctcc ggatcgtcgc cgagcttgct
     1321 gcggaggcgg cggatgtaca cccgaacgta gtccacctcg ttgacgtatt cccgtcccca
     1381 gaccctggcc agcagtgtgc cgtgcgtcag tgtctgcccg gcattgcgta ccaggtgata
     1441 gagcagcttg tattcggtcg gtgtgagttc taatcgaaca ccgttgagat gcacttcctg
     1501 cgcggaaaag tccatcgcca gatcgcccga ctggaaggat ggcgcccgga tcttgggcgc
     1561 cgacatgtcg agtcgccgga gtacggcctt gatccgggcg agcagctcca gatggtcgaa
     1621 cggcttcgta atatagtcat cggcgccgag ttccagcccc ttgatcttgc tcatggtggt
     1681 gtcttgcgcc gtcagcatga tcacgggtac gtcggagaat gctcggatct cccgtaagac
     1741 ttcatatccg tccatgcccg gcagcccgat atcgaggagc accaagtccg gcgcctcacg
     1801 ctccaccaga tcgagcgcgg catcaccgct gccggcatcg agaatctcga cctcgcgcca
     1861 ctgaagcatc atcccgagac gaaccgcctc gaccacttcg gtcgcgtcgt cgaccagcag
     1921 aatcttcatt cttcatcagc ctcgagtgtt agacgtctgg ggatagcaac cgtgaatgtg
     1981 cttccgcgac caacggcgct gtcaaccacg atcgtcccgc catgcgcctg ggcgatggcg
     2041 agcgcgattg gcaggccgag ccctgcgccg gggttgcgac cgacctcacc cgcctctcgc
     2101 gtaaagaagc gctcgaagag atgcacctgg tcctctggtc cgattccccg gccgttgtcc
     2161 gtcacggacc acgtggtccc ctccgcgtcg gcccgaagcg cgagcaagat cgacgcgccg
     2221 tcgggggaga acttggtcgc gtttgagagc agattgagca gcacctgctc gagccggccg
     2281 cggtcggcgt agatccagac gggatcagcc ggtacggtga gctttagaac ctgatcgcgg
     2341 gcctccatca gcgaagcgat cgacgagtgg acatcacggg taagcgcgcg cgcatcgaac
     2401 cgctgcggct
//
LOCUS       DASSGX010000247         2140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1136008, whole genome shotgun sequence.
ACCESSION   DASSGX010000247 DASSGX010000000
VERSION     DASSGX010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136008"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            625..>2140
                     /locus_tag="VFI12_04290"
     CDS             625..>2140
                     /locus_tag="VFI12_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055657.1"
                     /translation="MEMIRFDRRVLFGFAAALVTLVLLVGLGARSASAHDGHSDDPAI
                     TSYAQGEMTCGPDGLCSHGPDPAPPGVDPSVPDDPVDFQASSFAICDGNGVSGKRVQV
                     MYVHAADTPDRYSEFVDSFRGWAAEGDAVYQNSAQETGGMRYIRFVHDADCRITVLRI
                     TVSSTGDDNFWNLTDELKWQLGFTDPDRKYLVFLDRNHPDICGQADVIPWRADPSADD
                     PNPAYNPNNNTYGFAVIYNGCWSSNVTIAHELGHTFGAVQGTSPNHSQWDHCTDEWDV
                     MCYDDGSDSRFVLTYPCPDYSHNERLDCNHDDYFSTNPPAGSYLATHWNIANSGWLGT
                     SGARLSLDKEKSKFNGWITASMSGFSPGQQITLSWPDLTVIATATADSSGNATARFRT
                     PLAALGTYTIKAQDPAKTTATDLIRVIPRINLNEFSGPPGKEIRVYYYGYAPEDQVEV
                     RFYSLDGATYEVIGTVTVAANGRGTSLVTIPYAAEAGAHTIRGSVIGVGRSTTTT"
BASE COUNT          377 a          723 c          679 g          361 t
ORIGIN      
        1 ggctgaggaa cgaaaagccc actgaagggg ctgagttggg tcctttctcg acgatgtagc
       61 cccaggcggc ctcgcccggc tgcgtatcga ggagatccag tgcatcggac acgacccatc
      121 atcgccgccc cgggcctgaa ggccgggcgg atacctggaa ccggccctcg gggcacggtc
      181 atcgccctgc gggcgatcgc ggggcacaga ggcacccgcg ccacgttacg gcgcccccag
      241 ccggcttcag ccgggttcag atttcagccc gggtgtttac gcccgggtgg tatcgggcac
      301 gaacgtccgc atgccaacgc gccgattatg aagcggaggg cagatgccat ctgcccctac
      361 ccggtctcga gccatctgca accggcggtt ggggcagggc gggtttgccc ccgcaaaccc
      421 ctcaattcgt cttcgcgcct tcgcgccttc gcgcctttgc gactccgcgc ctcgtgtacg
      481 accagccgat gttgcagtac gctacgcgca attcggtaga tggacgcgca ggctggtgcg
      541 cgttggctgg ttcctcctca cgcccctccc gactcccccg cggaccggag ctcagtcgcg
      601 cgcccctgaa ggggtaatgt cgtcgtggag atgattcggt tcgatcgccg ggtgctgttc
      661 ggtttcgcgg cggcgctggt gacgctggtc ctcttggtcg gtctcggcgc gcgttcggcg
      721 agcgcgcacg acgggcattc ggacgatccg gcgatcacga gctatgccca gggcgagatg
      781 acctgcgggc cggacgggct ctgctcgcac gggccggacc cggcgccgcc cggcgtcgat
      841 ccctccgtgc cggatgaccc ggtcgacttc caggcgtcgt ccttcgcgat ttgcgatggc
      901 aatggcgtca gcggcaagcg cgtgcaggtc atgtatgtcc acgccgcgga cacgcccgat
      961 cggtacagcg aattcgtgga ttcattccgg ggctgggcgg ccgaaggcga tgccgtgtac
     1021 cagaacagcg cgcaagagac cggcgggatg cggtacatcc gcttcgtgca cgatgccgac
     1081 tgccgcatta cggtcctgcg gatcacggtc tcctcgaccg gtgacgacaa tttctggaac
     1141 ctgactgatg agctgaagtg gcagctcggc ttcaccgacc ccgaccgcaa gtacctcgtc
     1201 ttcctcgacc ggaaccaccc cgatatttgc gggcaggcgg atgtgattcc gtggagagca
     1261 gacccaagcg cggacgatcc caacccggcc tataacccga acaacaacac ctatggcttt
     1321 gcagtcatct acaacggctg ctggtcgagc aacgtcacga tcgctcacga gctggggcac
     1381 accttcggcg ctgtccaggg gacgtccccg aaccacagcc aatgggacca ctgcaccgac
     1441 gaatgggacg tcatgtgcta cgacgacggt tccgactcca ggttcgtgct gacatacccg
     1501 tgcccggatt acagccataa cgagcggctg gattgcaacc atgacgacta cttcagcacc
     1561 aacccgccgg ccggaagcta cctggcgacg cactggaata tcgccaacag cggctggctg
     1621 ggaacgagcg gggcccggct ctccctcgac aaggagaaga gcaagttcaa cggctggatc
     1681 accgcctcga tgagcgggtt cagtccgggc cagcagatca ccctctcctg gccggacctg
     1741 accgtgatcg ccactgccac cgccgactcg tcgggcaatg caacggcgcg gttccggaca
     1801 ccattggccg cgctcgggac ctacacgatc aaggcccagg atccggccaa aacgaccgcg
     1861 acggacctga tccgggtcat tccgcggatc aacctgaacg agttctccgg tcctcccggc
     1921 aaagagatcc gcgtctacta ctacgggtac gcgccggagg accaggttga ggttcgcttc
     1981 tactcgctgg atggcgcgac ctacgaggtg attggcacgg tcaccgtcgc cgccaatggt
     2041 cgcggcacct cgttggtgac gatcccctac gccgccgagg cgggcgccca cacgatccgg
     2101 ggctcggtga tcggggttgg ccgcagcacc acgacgacct
//
LOCUS       DASSGX010000248         2013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1136532, whole genome shotgun sequence.
ACCESSION   DASSGX010000248 DASSGX010000000
VERSION     DASSGX010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136532"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1090)
                     /locus_tag="VFI12_04295"
     CDS             complement(<1..1090)
                     /locus_tag="VFI12_04295"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET7055658.1"
                     /translation="MITPETPTPIRTPDLASFESVANPATPLEVAEILRGAADDGRAV
                     VPVGGGGALTIGNPTSAWAIGLSTRGMNRIIDYQPTDMTLSVEAGATLADVAAVLAEH
                     GQMLPIEAPLPASATIGGLLATALTGPRRYGGGTLRDVIIGISVSYPDGTVGKAGGLV
                     VKNVSGFDMMRIHYGALGTLGVITSANFKVMPIPRSEFTVTHPLRSLHEAGEILTMLR
                     SPSVRPVSLVLQLRDGTWTLIARFEGRESGLKAVASTVTGFLEVRDRHDDRESAAFWQ
                     RYMDDRAFADPAEVRLQVRTWPTGIVGVIDRALSVLYGTGRAEIEPGIGQAILSWSHT
                     TIDSASLIRSIRDATPGVSVTVLAAPDEV"
     gene            complement(1087..>2013)
                     /locus_tag="VFI12_04300"
     CDS             complement(1087..>2013)
                     /locus_tag="VFI12_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET7055659.1"
                     /translation="DGEVTWIESGQPGYDLTAAMVGSEGMLGIVTKARVKITPAPERV
                     SVILAAFPDIEAASQAVTAIISAGIVPAALEMMDALTIQAVEPAFHAGYPMDAGAVLL
                     IEIDGRPEDVQETQTEIELLCHQAQAMQVRSATSKVERDLLWKGRKMAIAAMGRLAPN
                     YYLHDTVVPRTRLPETMKRVYEIADDFSLPIANMFHAGDGNLHPLMLFDRHNKSEVER
                     VLEASKHLITFCVEIGGALTGEHGIGTEKRGYMRLVFDENDLAAMAGVKQTFDPTAMF
                     NPEKIFPSGYVCGEVRALRAQAIAQKHGIYPM"
BASE COUNT          344 a          665 c          624 g          380 t
ORIGIN      
        1 tgacttcgtc cggagcggct agcacggtta cagatacccc cggggtggca tcccgaatcg
       61 accgaatgag cgacgcgctg tcgatcgtgg tgtgcgacca tgagaggatt gcttgcccga
      121 ttcccggctc gatctcagcc cggcccgtgc catacaggac ggacaatgcc cggtcgataa
      181 cccccacgat ccccgtcggc caggtgcgaa cctggagccg gacttcggca ggatcggcaa
      241 atgcccggtc atccatgtat cgctgccaga atgcagccga ttcccgatca tcgtgtcgat
      301 ccctgacctc aaggaatcca gtgacggtgg acgcaacggc tttgaggccc gactcgcgtc
      361 cttcgaatcg cgcaatcagc gtccaggtcc cgtctcggag ttgcaaaacg agagagacgg
      421 gacgcacgct cggcgatcgc aacatcgtca ggatctctcc cgcctcatgg agtgacctga
      481 gcggatgcgt cacggtgaac tccgacctcg gaatcggcat taccttgaag tttgcggatg
      541 tgatcacccc gagtgtgccg agcgcgccgt aatgaatccg catcatgtcg aagccggaga
      601 cgttcttgac gacgagtccc ccggccttgc cgaccgtccc atccggatag gaaacgctga
      661 tcccgatgat cacgtcgcgc aatgtcccgc cgccgtaccg ccgtggtccg gtcaatgccg
      721 tcgccagcag tcccccaata gtcgcgcttg ccgggagcgg cgcctcgatc ggcagcattt
      781 gtccatgctc ggcgaggaca gcggcgacat ccgccagcgt ggcgccggcc tcgaccgaga
      841 gggtcatatc ggttggctgg tagtcgatga tccggttcat tccccgggta ctgaggccta
      901 tcgcccaggc gcttgtcgga ttcccgatcg tcagcgcacc accgccgcca actggcacga
      961 ccgcgcggcc gtcatccgcc gcgccgcgca ggatctcggc gacctcgagt ggcgtcgcgg
     1021 ggttggcgac cgactcgaac gaggcgagat cgggcgtgcg gattggtgtc ggcgtctcgg
     1081 gggtaatcac atcggataga tcccgtgctt ctgggcgatc gcctgcgccc tcagcgcccg
     1141 cacttcgccg cagacgtagc cgctcgggaa gatcttctca gggttgaaca tggcagtggg
     1201 atcaaaggtc tgtttgaccc ccgccatcgc cgccaggtcg ttctcatcga atacgaggcg
     1261 catgtagcca cgcttctcgg tgccgattcc gtgctccccc gtcagtgcgc caccgatctc
     1321 gacgcaaaac gtgatcaggt gcttgctcgc ttcgagtacg cgctcgacct ccgacttgtt
     1381 atggcggtcg aagagcatca acgggtgcag attcccatcg ccggcgtgga acatattggc
     1441 gatcggcagg ctgaagtcgt cggcgatctc atatacgcgc ttcatcgtct ccggcaggcg
     1501 cgtgcgcggc acgaccgtat cgtgcaggta gtaattcggg gcgagccggc ccatcgcggc
     1561 aatcgccatc ttgcggccct tccagagcag gtcgcgctcg accttgctcg tcgctgagcg
     1621 gacttgcatc gcctgtgcct ggtggcagag cagctcgatc tcggtctgcg tctcctgcac
     1681 gtcttcgggc cggccatcga tctcgatcag cagcaccgct ccggcatcca tcgggtaccc
     1741 agcgtgaaag gccggctcga ccgcctggat tgtcagcgcg tccatcattt cgagcgccgc
     1801 gggtacgatg cccgcgctga tgattgccgt taccgcctgg gacgccgcct cgatgtccgg
     1861 gaaggctgcg aggatgacgg atacccgctc aggggccggc gtgatcttga cccgcgcctt
     1921 ggtgacgatg ccgagcatgc cctcggaacc gaccatggcc gcggtcaggt cgtagccggg
     1981 ttgcccggat tcaatccagg tcacctcgcc atc
//
LOCUS       DASSGX010000249         5052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1354018, whole genome shotgun sequence.
ACCESSION   DASSGX010000249 DASSGX010000000
VERSION     DASSGX010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5052
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354018"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VFI12_04305"
     CDS             complement(<1..263)
                     /locus_tag="VFI12_04305"
                     /inference="COORDINATES: protein motif:HMM:NF020250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HET7055660.1"
                     /translation="MAIEIGSRDAVKGSVPFNDVAIILDPSDVVGIAKRPLLPGTVLV
                     RENGVELRIAQMIPPGHKFALVDIANDAPVRRYGQIIGFATAD"
     gene            631..912
                     /locus_tag="VFI12_04310"
     CDS             631..912
                     /locus_tag="VFI12_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055661.1"
                     /translation="MNTASILKEALISGITAGVVIPLLWVAGAAINPERDTHSPGLDL
                     IALTLAGAVMVMLLRSTWRGTAEAFPDHDYAAEEWDWRRLADSYGGGHC"
     gene            complement(1056..2183)
                     /locus_tag="VFI12_04315"
     CDS             complement(1056..2183)
                     /locus_tag="VFI12_04315"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HET7055662.1"
                     /translation="MLNGATEPRDNSPHDASFWTVIPAGGSGTRLWPLSRAARPKFLL
                     PLIKQEVSLIQETVDRITPLAPPERTLVVCGPAHTVGIARQLPQVPESNLVVEPSPRG
                     SGPAIILAAALIHRIDPTAIMGSFAADHDVTKRPAFEQAVRTAIETARDNWLVTIGIT
                     PTRPETGYGYIERSDAIVGKSNGSTAYQALRFVEKPPLETAQEYLASGRFLWNASMFI
                     WKCSTLLEFARTLMPDLLEAVTQIVDVWDTPDREVTINRIWDALPESTIDQGIMEKAD
                     RVAVVPADIGWSDVGDWHGLGELIEHDGLGNSVRGDLIQVETRRSVVWSETGRLVALV
                     GLENVVVVDCEDALLVIDRNRSQDVRKIVGELKRLSRNEFS"
     gene            2272..3198
                     /locus_tag="VFI12_04320"
     CDS             2272..3198
                     /locus_tag="VFI12_04320"
                     /inference="COORDINATES: protein motif:HMM:NF042587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HET7055663.1"
                     /translation="MRPRLDEKPWGGNRLAHFGIDLGDSDQIGEAVLTSNDAVVADGQ
                     YAGKTLGELVSLDPDGLLGPVARRFRGTGFPLLAKLIDAHENLSIQVHPTDDLAPENE
                     CGKTEAWFILEAAPGAVVYAGLQDDATGDEVRERLERSETIVPLVRAIPVAPGDALFL
                     PAGTIHALGAGIVLFEIQQQSAITYRLEDWGRKREMHVEAGMRALNPEHRPAPARAEM
                     RTAGTQPEPAVRCEYFALEVIQIGAGERIELPLESGPQVFTCVRGRAKLRAGEHSVSL
                     DAGSTAILFANAGAVRISSGSGGRLLRGWIGD"
     gene            complement(3151..3546)
                     /locus_tag="VFI12_04325"
     CDS             complement(3151..3546)
                     /locus_tag="VFI12_04325"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7055664.1"
                     /translation="MEGFVISLDDALTEHAATGGAYHQFFQVPEMSAGIYTLPARGVD
                     GQAPHVEDEIYYIVSGKAQVQIGDRHYPVQTGATIFVAREVEHRFHDIEEDLTILVIF
                     APAHDVEESNRRRTSVANPPAEETPTASA"
     gene            3607..4878
                     /locus_tag="VFI12_04330"
     CDS             3607..4878
                     /locus_tag="VFI12_04330"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7055665.1"
                     /translation="MRARFQSGVWALHSFRLLISASMISIFGSLITSTAFPFIAIHEL
                     DAGPAELALLSLAGIVPSIVLGSVAGIWIDRISRRRVMIASDLLSAAALLTIPIAYWL
                     DALSLAQLLAVTFVTSVGRLCFRIADRSFLPQVVGRAHVEEANATLSGGSALAEAGGF
                     SAGGLLVQLLTGPVALVFDALSFIGSALLLRKIPDRAAPLDLEGPVDARHWRSELAEG
                     LGFLRRSTTLLPLAASLFLMSIGIEAIGTVYFLFVNEELGFPVGALGFIFATGGIGSL
                     IGAAMSTRVTARFGAGPALIGSLVILAIGQGSIALATSVGLFAVVVMLGQQLTDAFWL
                     YYESTSMSLRQLNAPEAMLGRINGAFESLEFVGLLLGAGLGALLGEFVGLRATLVTGS
                     ALLAVAGLPLLLSPVRETREVTVAEVSSVAG"
BASE COUNT          801 a         1507 c         1807 g          937 t
ORIGIN      
        1 atgtcggccg tggcgaagcc gatgatctgg ccgtagcggc ggacgggcgc gtcgttcgcg
       61 atgtcgacga gggcgaactt gtgtcccggc gggatcattt gcgcgatgcg caactccacg
      121 ccattttcgc ggaccaggac cgtgcccggc agaagcggcc gcttggcgat gccgacgaca
      181 tcagacgggt caaggatgat ggcaacgtcg ttgaaaggga cgctgccctt gacggcgtcg
      241 cgggatccga tttcgattgc catggtggga tggacctttc cgccttgctc tgttcgtgac
      301 tccgtggccg acccgggcag caaggcagcc tgccacgggc gactcgggtg gactcgtgca
      361 gggcaaccgc cggtggcgcc ggagcgccac ggcaggctcg gcgagcgcac cgtcaatgct
      421 atccaaatga tacccgagca cgactgaatc cgggcgcggc cggatgtcaa acatggccag
      481 atcagagcgc gccgtcgatt cgtgaccctc gcgaacgact atgaggtgaa cgtcgttcat
      541 tgccgccgcc ggcctggcgc acatccggat cgcgggtcgg agccagatat ccagtgcgga
      601 atcggacagt gcaatgaaga ggagcattcg atgaacaccg catcgattct caaggaagcc
      661 ctgatttccg gcatcaccgc aggcgtggtc attcccctgc tctgggtcgc gggcgcagcc
      721 ataaatcccg agcgggatac gcattcgccc ggtctcgatc tgattgccct gacgctggcg
      781 ggcgccgtca tggtcatgct gctgcgatca acctggcgcg gtacggcaga ggcattcccg
      841 gaccacgatt acgcggccga agaatgggat tggcggcgtc ttgcggactc gtacggcggc
      901 ggccattgct gatcagcgca accgtcggag acggggtaac gaaattgtga ccaccccgtg
      961 atggaatccc ggctggcccg cccggccttg gtgaggcgca gccgggcgag gccgtccgga
     1021 attcctttgg gcggacgctt cgactgaaat gcgatttagc tgaattcgtt gcggctgagg
     1081 cgtttgagct cgccgacgat cttgcggacg tcctgcgagc gattgcggtc aatgaccagg
     1141 agggcgtcct cgcaatcgac gacgaccacg ttttcgaggc cgacgagggc gacgaggcgg
     1201 ccggtttcgg accagacgac gctacgacgg gtctcgacct ggatgagatc gccgcggaca
     1261 ctgttgccga gcccgtcgtg ctcgatcaac tcgccgagac cgtgccaatc accgacatcg
     1321 ctccagccga tatcggccgg gacgaccgcg acacgatcgg ccttttccat gatgccctga
     1381 tcgatcgtcg attccggcag ggcgtcccag atccggttga tcgtgacctc ccgatcgggc
     1441 gtgtcccaga cgtcgacgat ctgagtgacg gcttcgagca gatccggcat cagggtccgg
     1501 gcgaactcga gcagggtcga gcatttccag atgaacatgc tcgcgttcca gaggaagcgg
     1561 ccgctggcga ggtattcctg cgccgtctcg agaggcggct tctcgacgaa gcggagcgcc
     1621 tggtaggcgg tcgagccgtt gctcttaccg acgatcgcgt cgctgcgctc gatatagccg
     1681 tagccggtct cggggcgggt cggggtgata ccgatggtga ccagccagtt gtcccgggcg
     1741 gtctcgatcg cggtgcggac agcctgctcg aaggccggcc gcttcgtcac atcatgatcg
     1801 gcggcgaagc tgcccatgat cgcggtcggg tcgatccggt ggatcagggc ggcggcaagg
     1861 atgatggcag ggccggagcc gcgcggcgat ggctcgacga cgaggttgct ttccggcacc
     1921 tgcgggagtt ggcgcgcgat cccgaccgta tgggccgggc cacagacgac gagcgtgcgc
     1981 tccggcgggg ccaggggggt gatgcgatcc accgtctcct ggatcagcga aacctcctgc
     2041 ttgatcagtg gaagcagaaa cttcgggcgc gcggcccggc tcaggggcca caggcgcgtg
     2101 cccgagccgc cggccgggat cacggtccag aacgatgcgt cgtgcgggga attatcgcgg
     2161 ggctcggtcg ctccgttgag catggctggt atcacctgtg attgacagtc ggatgcctac
     2221 tcgacattat gaaggtctat ggaacccgtc aagatatctg agccactgtt ggtgcgaccc
     2281 cgccttgacg aaaagccgtg gggcggtaat cgccttgccc acttcgggat cgacctcggt
     2341 gacagcgacc aaatcggcga agccgtcctg accagcaatg acgccgtcgt agccgacggg
     2401 cagtatgccg ggaagacgct cggcgaactg gtctcgctcg atccggacgg gctgctcggt
     2461 ccggtggcgc ggcgatttcg cgggaccgga ttcccgctgc tggcaaagct gatcgacgcg
     2521 catgagaacc tctcgatcca ggtccacccg accgacgacc tcgcgcccga gaacgaatgt
     2581 ggcaagaccg aggcctggtt catcctcgag gcagcgccgg gcgcggtcgt ctatgccggg
     2641 ttgcaggacg acgcgaccgg cgatgaggtg cgggagcggc tggagcggag cgagacgatc
     2701 gtgccgctgg tgcgggcgat cccggtcgcg ccgggggacg cgctcttctt accggcggga
     2761 acaattcacg cgcttggggc gggtatcgtc ctcttcgaga tccagcagca gtcggcgatc
     2821 acctaccgcc tcgaagactg gggccggaag cgcgaaatgc atgtcgaggc cgggatgcgg
     2881 gcgctcaacc cggagcaccg gccggcgccg gcccgggcgg agatgcggac ggcgggaacc
     2941 caaccggagc ctgcggtacg gtgcgaatac ttcgcgctcg aggtgatcca gatcggggcc
     3001 ggcgagcgga ttgaactccc gctggaatca gggccccagg tcttcacctg cgtgcgtggc
     3061 agggcgaaac tccgggccgg cgagcattcc gtgtcgcttg atgccggttc gacagcgatc
     3121 ctgttcgcga atgccggggc agtccgaatc tcaagcggaa gcggtgggcg tctcctccgc
     3181 gggtggattg gcgactgagg tgcggcggcg gttcgattct tcgacgtcgt gggccggggc
     3241 gaagatgacc agaatggtca ggtcctcctc gatgtcatgg aagcggtgct cgacctcgcg
     3301 ggcgacgaag atggtcgcgc cggtctggac cgggtagtgg cggtcgccga tctggacctg
     3361 cgccttgccg gagacgatgt aatagatctc atcctcgacg tggggggctt gcccatcgac
     3421 cccacgtgcc ggtaatgtgt agatgccagc gctcatctcg ggcacctgaa agaactgatg
     3481 gtacgcgccg cctgttgcgg cgtgttccgt cagcgcgtcg tcgagcgata tgacgaatcc
     3541 ctccatcacc tctccacctt tctgctagac tcgccggccg caatcataac gaagcatgga
     3601 tggccaatga gagcccgatt tcagagcggc gtctgggcgc tgcactcgtt caggctgttg
     3661 atctccgcgt cgatgatctc gatcttcgga tcgctgatta cgtcaactgc attccccttt
     3721 atcgcgattc acgaactcga cgccggaccg gcggagctgg cgttattgag cctggcggga
     3781 atcgtgccga gcatcgtgct gggctcggtc gcggggatct ggatcgaccg gatctcgcgc
     3841 cgccgggtga tgatcgcgtc cgatctgctc agcgcggcgg cgttgctgac gattccgatc
     3901 gcctactggc tcgatgcgct gagcctggcc cagctcctgg ccgtcacgtt cgtgacgagc
     3961 gtcggccggc tctgcttccg gatcgccgac cggtcgttcc tgccgcaggt ggtcgggcgg
     4021 gcgcatgtcg aagaggcgaa cgcgacgctc tcgggcggct cggcgctggc tgaggcaggt
     4081 ggattcagcg ccggcgggtt actggtgcag ctgctgaccg ggccggtggc gctcgtcttc
     4141 gatgcattga gcttcatcgg gtcggcactg ctgttgcgga agatcccgga ccgggccgcg
     4201 ccgctcgatc tggaagggcc ggtcgatgcc cggcattggc gatcggaact ggcggagggg
     4261 ctcggtttct tgcggcgctc gacgacgctg ctgccgttgg cggcatcgct cttcctgatg
     4321 tcgatcggca tcgaggcgat cgggacggtc tacttcctgt tcgtgaacga ggagctgggc
     4381 ttcccggtcg gggcgcttgg gttcattttc gcgaccggtg gcatcggctc tttgattggg
     4441 gcggcgatgt cgacgcgagt gacggcccgg tttggggcag gtccggcgct gatcggctcg
     4501 ctggtgatcc tggcgatcgg acaggggtcg atcgcgctgg cgacgtcagt cgggttgttt
     4561 gcggttgtcg tgatgcttgg gcagcagctg accgatgcct tctggctcta ttacgagtcg
     4621 acctcgatgt cgctgcggca gctcaatgca ccggaggcga tgctggggcg gatcaacggc
     4681 gcgttcgaga gcctcgagtt cgtgggattg ctgcttgggg cggggttggg cgccttgctc
     4741 ggcgagttcg tcgggttgcg ggcgacgctg gtgacggggt cagccttgtt ggcggtggcc
     4801 gggttgccgt tgctgctgtc gccggtccgg gagacgcggg aggtgacggt ggccgaggtg
     4861 agctccgtcg cggggtaaat cggggcgagc gacgttcccg gtgaacgggt gatccaacag
     4921 gtaacgtggc ggcgggagaa cggtgtcgca ttgcgatgcg acgctacagg tgctcgttgg
     4981 ggcgggatcc gtccggggcg ggcagatgcc atctgcccct acatcgtctg cgcgcctccg
     5041 cggcgcgggc tt
//
LOCUS       DASSGX010000250         2813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_265103, whole genome shotgun sequence.
ACCESSION   DASSGX010000250 DASSGX010000000
VERSION     DASSGX010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265103"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            256..>2813
                     /locus_tag="VFI12_04335"
     CDS             256..>2813
                     /locus_tag="VFI12_04335"
                     /inference="COORDINATES: protein motif:HMM:NF021570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HET7055666.1"
                     /translation="MSIRRAAPFHRSRLDLLALVAILISAFSLRVYDLNWDQGKFLHP
                     DELFVTIVSNDRIHFDWPPDWNAIRNPDISPLNPRSKTCGDQQNSVCNYSYGALPLLI
                     TDFAATSLAELTGTPWDNFDHIPRVGRSISAFVDTITVLLVFLVASRLFDRRAGLLSA
                     AIYAGTPLAIQLSHFYTTDIWMACFVTLTLWCSLLALDRERLGWFALAGAAFGFAMAS
                     KGSSLLLAGVVALAALVVAWRHFDSDDPTDAITSAIGRLAVSCLAAIAAFAVFEPYAL
                     LRMRIYVDQIREQQQMSSGQIDFPFTRRYVGTTPVLYQLEQLTKWGMGPVATILGFVG
                     VALMAWWCLRRRRADILLVLVWLGLQSIVILTPQVKYLRYEIPIVPVLAIGGGVAIWA
                     GVRWLSARWNRGFAAAFATACLAGMAVWTLAYVSIFNGDHPRIAASKWIYANVPMGSA
                     VSSESWDDSLPVSFGPMLSRGDMQYQEVSFDIYGDRPQQEVADQLYADLEQSDYVVLS
                     SNRLIESVRQLPWRYPVQIRYYDLLQSGQLGFDLVADFHTEPKIGPFKFDDSLADESW
                     VNYDHPRVLIYKKTTLVDKDAYQTLMIPAIQADWTPTRLNDQQKSLMLDEPVGQLPAV
                     DNFRWSERWTDNSAVALGVWIVLLVLFGAVGRQWARLLFPRFPDGGAGMSRVLTLILA
                     GWLLWFLASLKVISFSVLWCWAALVVVAAVGFALWWFLADRDRAFPRPAIAGAEVAFW
                     AVFALFLLFRWINPDSWHPIWGGEKPMEFAHLNATLRSAHFPPYDPWFSGGYINYYYY
                     GLYLVAYCIKLTGIPAEIAFNLAQPTMMGLAASGAYTLASALGAGRRRVAQSVAT"
BASE COUNT          424 a          920 c          920 g          549 t
ORIGIN      
        1 cctcctcgtg catccccccg cgcagttcat gacgtcgatc cctgccacgg cgtttggttc
       61 cccggtattg ccgggttcgg gcggcccacg gggggaccgc gctacgcccc tgaccaggca
      121 ctggctctgc ccgccgcgcg ggttgatcga cgatgtcggc catgggggac cggcgctccg
      181 ttgtttgtgc gccggcgggc gaaaaacaat gcgcgggtcg accgggccaa acgcaaatcg
      241 tacaatccgc gcatcatgtc aatccggcgc gccgcaccct tccaccggtc cagacttgat
      301 ttgctggcac tggtggctat cctcatcagc gcgttctccc tgcgtgttta cgatctgaac
      361 tgggatcagg gcaagttcct gcaccccgac gagctgttcg tcacgatcgt ctccaacgac
      421 cggatccact tcgattggcc gccggattgg aatgcaatcc ggaatccgga catctcgccg
      481 ttgaacccgc gatcgaaaac ctgcggagac cagcagaact cagtctgcaa ctattcctac
      541 ggcgcgctgc cgctgttgat caccgacttc gcggcgacgt cgctggccga gctcaccggc
      601 acgccttggg acaacttcga tcacattcca cgcgtcgggc ggtcgatttc cgcgttcgtg
      661 gacaccatca cggtgctgct cgtcttcctg gtggcgagcc ggttgttcga ccggagggca
      721 ggcctgctct cggcggcgat ctacgccgga acaccactcg cgattcagct ctcccacttc
      781 tacacgaccg acatctggat ggcctgcttc gtcacgctca ccctctggtg ctcgctgctg
      841 gcgctggacc gggaacggct cggctggttc gcgctggccg gggcggcctt cggcttcgcg
      901 atggcgtcga aggggagcag cctgctgctg gccggagtcg tggcgctggc ggcgctggtc
      961 gtcgcctggc ggcacttcga ctccgacgat ccaaccgacg caatcacgag cgcgatcggc
     1021 cggctggcgg tcagctgcct ggcggcgatc gcggccttcg ccgtcttcga gccgtacgca
     1081 ttgctgcgga tgcgaatcta tgtcgatcag atccgcgagc agcagcagat gtcgagcggg
     1141 cagatcgact tccccttcac gcgccgctat gtcggcacga cgcccgtcct ctaccagctt
     1201 gagcagctga cgaaatgggg catgggaccg gtcgcgacga tcctcggctt tgtcggcgtg
     1261 gcgctcatgg cctggtggtg cctgcggcgg cggcgagccg acattctgct ggttctggtc
     1321 tggctcggac tgcagtcgat cgtgatcctg acgccgcaag tcaagtacct acggtatgag
     1381 atcccgatcg tgccggtgct ggcgatcggc ggcggcgtgg cgatctgggc gggtgtgcga
     1441 tggttgtccg cacgctggaa ccggggcttc gcggcagcct tcgcgaccgc ctgcctggcc
     1501 gggatggcgg tctggacgct cgcctacgtc tcgatcttca acggcgacca tccccgaatc
     1561 gcggcctcga agtggatcta cgccaatgtg ccgatgggca gcgcagtgtc gtccgagagc
     1621 tgggatgact cgctgccggt ctcgttcggg ccgatgctca gccgcggcga catgcagtac
     1681 caggaggtct cgttcgacat ctacggcgac cgcccgcagc aggaggtcgc tgatcagctc
     1741 tacgcggacc tcgagcaatc cgattatgtc gtgttgtcgt cgaaccggtt gatcgagtcg
     1801 gtccggcagt tgccgtggcg ctacccggtc cagatccgct attacgactt gctccagtcc
     1861 ggtcagcttg ggttcgacct ggtggccgat ttccatacgg aaccgaagat cggaccgttc
     1921 aaattcgatg attcgcttgc cgacgaatcg tgggtgaatt acgaccaccc gcgcgtcctg
     1981 atctacaaga agacgaccct cgtcgacaag gacgcgtacc agacactgat gataccggcg
     2041 atccaggcgg actggacgcc gacgcggctc aatgaccagc agaaatcgct gatgctggac
     2101 gaaccggtcg ggcaattgcc ggccgtcgac aacttcagat ggagcgagcg ctggaccgac
     2161 aacagcgcgg tcgccctcgg cgtctggatc gtgctgctgg tgctgttcgg cgcggtcggg
     2221 cggcaatggg cgcgattgct cttcccgcgt ttcccggacg gcggcgcggg catgagccgg
     2281 gtgctgacgc tgatcctggc cgggtggctg ctctggttcc tggccagcct gaaggtgatc
     2341 tcgttcagcg tgctgtggtg ctgggcggcg ctggtcgtcg tcgcggcggt cgggttcgcg
     2401 ctctggtggt tcctggccga ccgggaccgc gccttcccga gaccggcgat cgcgggggcc
     2461 gaggtcgcct tctgggccgt cttcgcgctc ttcctgctct tccggtggat caaccccgac
     2521 tcgtggcacc cgatctgggg cggcgagaag ccgatggagt tcgcgcacct caacgcgacg
     2581 ctgcggagcg cgcacttccc gccgtacgac ccgtggttct ccggcgggta catcaactat
     2641 tactactacg gcctctacct ggtcgcctac tgcatcaagt tgaccgggat cccggccgag
     2701 atcgccttca acctcgccca accgacgatg atgggcctgg ccgcatccgg cgcgtacacg
     2761 ttggcgtcgg cgctcggggc cggacggcga cgggtggcgc agagtgtcgc aac
//
LOCUS       DASSGX010000251         2337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1573677, whole genome shotgun sequence.
ACCESSION   DASSGX010000251 DASSGX010000000
VERSION     DASSGX010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1573677"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="VFI12_04340"
     CDS             complement(<1..562)
                     /locus_tag="VFI12_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055667.1"
                     /translation="MPAAIGHGRESQARASQPEDEDRLPREDEEIDPGVGYACPMNRI
                     DRFRMDIDERHPLIFDAAIAGLLLALSLVTLKALWPLTDQEHPIRAVGITSLALVPLI
                     WRRRFPLAVLAAMSVVFTVGHALAVPETEWWTYSWAVAFYGAGVYGSRRHRNIVRLGA
                     VAVFGGVLIHRLFFREIGDFEGNLALL"
     gene            843..1619
                     /locus_tag="VFI12_04345"
     CDS             843..1619
                     /locus_tag="VFI12_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7055668.1"
                     /translation="MLWFLTRKDSSSNLVTGPDQNLIIEARDIVKTYNTGTEKVSALK
                     GISFGVAPGEMVAVMGPSGCGKTTLLNCLSGLDNIDSGNIWLEGEDLATMKDRKRTSY
                     RAKRMGFIFQVYNLLPVLSAVENVELPLLVSGVTPKIARQKAIAALETVELSDRANHR
                     PAELSGGQRQRVTIARSLVNDPAIVWADEPTGALDSKTATDIMALIGKLNEENGLTFV
                     LVTHDRGIGAECDRVVHMRDGEIVDQTINRPAAYTESAAD"
     gene            1676..>2337
                     /locus_tag="VFI12_04350"
     CDS             1676..>2337
                     /locus_tag="VFI12_04350"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7055669.1"
                     /translation="MNELFGIPMSGILVALVTLLAICLLTVAWVAWRRPVIFKLGVRN
                     IPRRKAQTILIVVGLMLSTLIISAALGTGDTLDYSTTSEVYTSLGNVDEVVVYSPDGK
                     GDAATSLSSNIDTTALATVETALAGDEHVDGMMPVLWKTVPVLNATSGLAEPEVGVTG
                     LDPSRLAPFGGIEGTNGREIDLSALAPNEIVVNETTADKLNAQVGDTLVVYYNNRPVQ
                     LTV"
BASE COUNT          464 a          767 c          735 g          371 t
ORIGIN      
        1 ggagcagcgc caggttgccc tcgaaatctc cgatctcccg gaagaagagg cgatggatga
       61 ggacaccgcc gaacacagcg acggcgccga gccggacgat attgcgatgc cggcgcgaac
      121 cgtagacgcc ggcgccgtag aaggccacag cccaggaata ggtccaccat tccgtttccg
      181 gcaccgcaag ggcatgcccc acggtgaaga cgacggacat cgcggccagc accgccagtg
      241 ggaaccggcg gcgccagatc agcggcacaa gcgcgaggct ggtgatgccg acggcccgga
      301 tcgggtgttc ctggtccgtc agcggccaaa gcgccttgag cgtgaccagc gacagcgcca
      361 gcaagagacc ggcaatcgcg gcgtcgaaga tcagcgggtg gcgctcatcg atatccatgc
      421 ggaaacggtc gatccggttc atagggcaag catagccaac gcccgggtcg atctcctcat
      481 cctcgcgggg gagacgatcc tcatcctcag gttgagatgc acgcgcctgc gattcccgcc
      541 cgtgaccgat ggcggccggc actcgctccc gcagacttct tgcagcgcaa cgatgcgtcg
      601 tcgtcgcccc gccgggtccc ggatctccgc cttttgacgg agaccgggca tcggccggag
      661 gtacccgctt cgtgtcggcc ctttccccga cgtcaacgga gtcgatcacg atcagaattg
      721 tcaacagata acgcaaaacg cgagcccggc gaatcccggg tctgtccgag agaagttggc
      781 gactgccaac caccattcgc gcgcaccagg aatcagacga tcagatgaaa aggagaccca
      841 gcatgctttg gtttttgacc cgcaaggatt cgagctcgaa cctggtcacc ggccccgatc
      901 agaacctgat tatcgaggcg cgggatatcg tcaagaccta caacaccggt acggagaagg
      961 tatccgcgct caagggcatc agcttcggcg tggcgcccgg cgagatggtc gcggtcatgg
     1021 gcccctccgg ctgcggcaag acgacgctgc tcaactgcct ctcgggcctc gacaacatcg
     1081 actccggcaa catctggctt gagggcgagg acctggcgac catgaaggac cgcaagcgga
     1141 cctcgtaccg ggcgaaacga atgggcttca tctttcaggt ctacaacctg ctgccggtgc
     1201 tgagcgcggt cgaaaatgtc gagctcccgc tgctggtatc gggcgtgact ccgaagatcg
     1261 cgcggcaaaa ggcgatcgcg gcgctggaga cggtcgagct gtcggaccgg gcgaatcacc
     1321 ggccggccga gctttcgggc gggcagcggc agcgtgtcac gatcgcgcgg tcattggtca
     1381 acgatccggc catcgtctgg gcggacgagc cgaccggcgc gctcgactcg aagacggcga
     1441 ctgacatcat ggcgctgatc ggcaagctca acgaggagaa cggcctgacc ttcgtgctgg
     1501 tcacgcacga ccgcggcatc ggcgccgagt gcgaccgggt cgtccacatg cgggacggcg
     1561 agatcgtcga tcagaccatc aaccggccgg cggcctacac cgagtccgcg gccgactaac
     1621 gcgcgatccg atcttcatcc aactcgtatc catcgcctga gagaggacgc ttcccatgaa
     1681 tgaactgttt ggaatcccga tgtcgggaat cctcgtggcg ctggtaaccc tgctcgccat
     1741 ctgcctgctg accgtggcct gggtcgcctg gcgccgcccg gtcatcttca agctcggcgt
     1801 gcgcaacatc ccgcgccgca aggcccagac catcctgatc gtcgtcggcc tgatgctctc
     1861 caccctgatc atctcggctg cccttggcac cggcgatacg ctcgattatt cgacgacctc
     1921 cgaggtctac acctcgctcg gaaacgtcga cgaagtcgtt gtctacagtc cggatggcaa
     1981 gggggacgcc gcgacgtcgc tgagctccaa cattgacacc accgccctgg ccacggtcga
     2041 gaccgcactg gccggcgacg agcacgtcga cggcatgatg ccggtactct ggaagacggt
     2101 tcccgtcctg aatgccacga gcggcctggc ggaacccgaa gtaggagtga ccgggctcga
     2161 tccaagccgc ctcgcgccgt ttggcggtat cgaggggacg aacgggcgcg aaatcgatct
     2221 gtccgcactc gccccgaacg agatcgtcgt caacgagacg accgccgaca agctgaacgc
     2281 gcaggtcggt gacacgcttg tcgtctatta caacaaccgg ccggtacagc tgaccgt
//
LOCUS       DASSGX010000252         4373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1137820, whole genome shotgun sequence.
ACCESSION   DASSGX010000252 DASSGX010000000
VERSION     DASSGX010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137820"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..814
                     /locus_tag="VFI12_04355"
     CDS             <1..814
                     /locus_tag="VFI12_04355"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HET7055670.1"
                     /translation="NAPSYIGRGLIIQRNTPREVRGRVASSFAVTRDVAFMIGMAMAG
                     LADLAGEQALVLVCGGVLLIAGVVAAAMPGLGQPTDEWRRVVAMLRNAQEAPGLGLGR
                     GALPADLERLATHLPAFGMNNIERQALARQLRVFEVPAGTSIVRTGDPSTAAYFLLDG
                     RTVATRDGRILEVHNDGDFFGEIAALTGTPRTADVLAEQATTLLQAPAPVLRRLMDDP
                     ALQRLLLSKMTERLVRLDLLELPRMVGLDQSALRELRAADAESPTPVLATAT"
     gene            877..1572
                     /locus_tag="VFI12_04360"
     CDS             877..1572
                     /locus_tag="VFI12_04360"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET7055671.1"
                     /translation="MTALSIEDRLLRVPIFANLSAEALAELVHASRVRRFPSGQVIFS
                     EGDPGDHLVVLEEGQLKICRYSSSGEEIVLAVVDPPAIVGELALLDGAPRDATVIAQR
                     SVVVRLVPRDAFLSVLSTPTAVDGLLRTLAGWIRLANRRHADLIGLGVPGRLAKWLLE
                     QAGDVSGEQLRGGAVVELGRTQGQLAAELGTTRSTLNRALQGFADLGLLEVEPGGERV
                     TLRQPREMAAHVQ"
     gene            1618..2451
                     /locus_tag="VFI12_04365"
     CDS             1618..2451
                     /locus_tag="VFI12_04365"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET7055672.1"
                     /translation="MADNVSDGDVVNQAAADAVVARLLREAVWNVAATQALNAATPHE
                     NLIDLIVRTAARAIPSPEGALFLVDLERRVLTFDTVIGKTATAVLGTTIPLGHGIAGL
                     VAVSGQPLAVADAQLDPRHARDVAERSHYFPTSILAVPVVSRDGSVVGVLELLDRQGQ
                     PTYDLADIELLGFFASQLAIVLEQRQSIASATALVAHAIRNLTGIPEDAARALQAQIT
                     KAADDADATGGGTRQLAELVARIASRGPDEQTACRAVLAAFADYALGKAGSSAEGEWA
                     Y"
     gene            2544..3314
                     /locus_tag="VFI12_04370"
     CDS             2544..3314
                     /locus_tag="VFI12_04370"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET7055673.1"
                     /translation="MITRETAWGGSTGAGVRVAVIDSGIDADHPAVGAVAGYVAVSMA
                     ADGRLTFDDSPHADAYGHGTACAGIIRALAPDCKIYSVRVLGEDLTGRGAVFAAGLRW
                     SIDNGMDVCNLSLSTGRSEFFAVFHELADQAHFRDIPLVCAANNAPRPSYPSLFASVL
                     SVAAHEGADAEQFDVNLSPPVEFGAPGLDVPVAWLGGATITATGNSFAAPHITGLVAR
                     ILAKNPGLTVYQLKTVLRALAANAPRDPETDCARSIEP"
     gene            3443..3886
                     /locus_tag="VFI12_04375"
     CDS             3443..3886
                     /locus_tag="VFI12_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055674.1"
                     /translation="MTRKESNDAFNPAFDKMAASAFQGSGPAFGDVEAEVEGHILDDK
                     AYEANAFQSDGPAFGSDDAEVEGHIVDDKAYEANAFQSDGPAFGDDEAAAEVEGHLHP
                     ILMEDYSRVQAANSEAAIARAQRASEARTDRDGGVLDKVLRRKSK"
     gene            4029..>4373
                     /locus_tag="VFI12_04380"
     CDS             4029..>4373
                     /locus_tag="VFI12_04380"
                     /inference="COORDINATES: protein motif:HMM:NF023178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HET7055675.1"
                     /translation="MITADQLLAHAVGDYVLQSDWMATNKTSRTIVALIHALCYAIPF
                     LLFSPSALGLAVIIGSHLVIDRFRLAGWLNWLKNGLPRPRTPTGFPADRPEYLVVWLV
                     IITDNILHIAING"
BASE COUNT          741 a         1358 c         1464 g          810 t
ORIGIN      
        1 gaacgcgccg tcatatattg ggcgcgggct gatcatccaa cgcaacaccc cacgagaggt
       61 gcgggggcgc gtggccagct cgttcgcggt cacacgcgat gttgccttca tgatcgggat
      121 ggcgatggcg gggctcgcgg acctcgccgg ggagcaggcg ctcgttctcg tgtgtggtgg
      181 ggtgctgctc atcgcggggg tcgtcgcggc cgcgatgccg ggcttgggac aaccgacgga
      241 cgaatggcgt cgcgtggtcg ctatgcttcg caatgcccag gaagcacctg gccttggtct
      301 tggtcgggga gcgctcccag ccgacctcga gcgactggcg acacatcttc cggccttcgg
      361 aatgaacaac atcgagcggc aggcgcttgc tcggcagctc cgagtgttcg aggtccccgc
      421 tgggacgtcg atcgtcagaa ccggggatcc gagcaccgcc gcgtacttcc tgcttgacgg
      481 gcgcacggtc gccacgcgag atggacgaat cctcgaggtg cacaacgacg gcgacttctt
      541 tggagagatc gcggcgctga ccggcacgcc gcgcaccgcg gatgtgctgg ccgagcaggc
      601 cacgactctg ctccaggcgc ctgccccggt gcttcgtcgg ctgatggatg acccggcgct
      661 gcagcggctc ctcttgtcga agatgactga gcgcctggtg cggctggacc tcctcgaact
      721 cccgcgcatg gtgggtcttg accagtcggc cctgcgcgaa ctgcgtgctg ccgatgccga
      781 atcacccacg cctgtcttgg cgacggcaac gtagccgagg agttggcccg caggtaagaa
      841 tggcccgcat gactgtgcac ggaagcgaga caaggaatga ctgcactttc catcgaggac
      901 cgactgttac gggtgccgat ctttgccaat ctgtccgccg aggccctggc cgagctggtc
      961 cacgcctccc gagttcggcg atttccgagc ggccaggtga tcttttccga gggggatccc
     1021 ggcgaccatt tggtagttct cgaagaagga cagctcaaaa tatgccgcta ttcctcgtcc
     1081 ggcgaggaga tcgtcctcgc ggtggtggac cctccggcga tcgtcggtga actcgcgctg
     1141 ctcgacgggg cgccgcgtga tgcaactgtc atcgcgcaac ggtctgtggt agttcgactc
     1201 gttccgcgcg atgcgttcct gtcggtgctc agcaccccca ccgcggtgga tggactccta
     1261 cgaacgctgg cgggttggat tcgactggcg aaccgccggc atgcggacct cattggtctc
     1321 ggcgtccccg gacgattggc gaaatggctg ttggagcagg ctggcgatgt ttccggcgag
     1381 caattgcgcg gaggcgccgt ggtcgaactc ggccggacac agggacagct tgccgccgag
     1441 ctcggcacta cgcgaagtac gctcaaccgc gcgctccagg ggtttgccga tctcggactc
     1501 ctcgaggtcg aacccggtgg cgaacgagtg acgctgcggc aaccgcgaga aatggcggcc
     1561 cacgttcaat agtcaattgc ctggcgcgtc gaaggtctcg cgatggagtt ggttgagatg
     1621 gcggacaatg tgtctgatgg ggacgtcgtc aaccaggcgg ctgccgatgc agtggtggct
     1681 cgcttgctgc gcgaggccgt gtggaacgtc gccgcgaccc aggcgctgaa cgcggctacc
     1741 ccgcacgaga atctgatcga cctcatcgtt cggacggcag cgcgggcaat tccctcgccc
     1801 gaaggggcgc tctttctcgt cgacctcgaa cggcgcgtgc tgacgttcga cacagtgatc
     1861 ggcaagacgg cgacggcggt gctgggcacg acgatccctc tgggtcatgg catcgcgggc
     1921 ctggttgcgg tcagcgggca gccgctggcc gtggcggatg cccagcttga tcctcgacac
     1981 gcacgcgacg tcgctgagcg atctcactac tttccaacgt cgatcctggc tgtcccggtt
     2041 gtctcgcggg acggatcagt cgtcggcgtg ctcgagctgc ttgatcggca ggggcagccc
     2101 acctacgatc tggccgacat cgagctgctt gggttcttcg ccagtcagtt ggcgatagtg
     2161 ctcgagcagc gtcagtccat cgcgtcggcc acggcccttg ttgcccatgc gatccggaac
     2221 ctcaccggga tccctgagga tgctgctcga gcgctgcagg ctcaaattac gaaagccgcg
     2281 gatgatgcgg atgccaccgg cggagggacg cggcaactcg ccgagctcgt cgcgcgcatc
     2341 gcctcccgcg gcccggacga acagaccgca tgcagggccg tgcttgcggc gttcgccgac
     2401 tacgcccttg gtaaggcggg gagcagcgcc gaaggtgagt gggcatattg agcgctggat
     2461 tggggccgaa gccagcctgg tcagatgcgt tcgctcccgg tcgtctcgag cctttgccac
     2521 gcgtcaacct gtttggccga ggcatgatca cacgggagac agcatgggga ggcagcaccg
     2581 gcgctggcgt tcgggtcgct gtaattgata gcgggatcga cgcggaccac cccgcggttg
     2641 gcgcggtggc aggctacgtc gcggtttcaa tggcggcgga cgggcggctc acgtttgatg
     2701 actccccgca cgccgacgcc tatggccatg gcacggcctg cgccggcatc atccgggccc
     2761 tggccccgga ttgcaagatc tatagcgttc gggtcctcgg agaggacctg accggacggg
     2821 gcgcggtctt cgccgccggg ctgcgctggt cgatcgacaa cggaatggat gtgtgcaacc
     2881 tgagcctcag caccggtcgt tccgagttct tcgcggtctt ccacgagctg gccgaccagg
     2941 ctcactttcg cgatatcccg ctggtctgcg cggcgaacaa cgcgccccgt ccgagttatc
     3001 catcgctctt cgccagtgtg ctctcggtcg ccgctcacga aggcgccgat gcggagcagt
     3061 tcgacgtcaa tctctcgccg cccgtcgagt ttggcgcgcc gggacttgac gttccggttg
     3121 cctggctagg tggcgcgaca atcacggcca cggggaacag cttcgcggcc ccgcatataa
     3181 cgggattggt cgcgcgcatc ctcgccaaga acccgggact gacggtctac caactcaaga
     3241 cagtcttgcg cgcgctcgcg gcaaacgcgc cgcgggatcc ggagaccgat tgcgcgcgct
     3301 cgatcgagcc ataaggaggt gtgtcgaacg gcactgtgcc agggtgcggc gcaatgctgc
     3361 atcgagagtc gaacgaggct aggatgcggg tgtggtcggt ggcgacctcg gattccgcaa
     3421 gacgaagagg agaacaacgg cgatgacgcg caaagagtcc aacgatgcgt tcaatcctgc
     3481 attcgacaag atggcggcaa gcgctttcca gggctctggt ccagcgttcg gcgacgtcga
     3541 ggctgaggtc gaagggcaca ttctcgacga caaagcgtat gaggcaaatg cgttccagag
     3601 cgatggtccg gcgttcggca gcgacgacgc tgaggtcgag gggcacattg tcgacgacaa
     3661 agcgtatgag gcaaatgcgt tccagagcga tggtccggcg ttcggcgacg acgaggctgc
     3721 cgcggaggtc gaggggcatc ttcatccgat cttgatggaa gactactccc gcgttcaagc
     3781 agccaactcc gaagctgcga tcgctcgagc gcagcgggcg agtgaagccc ggaccgatcg
     3841 cgacggtgga gtcctcgaca aagtccttcg ccggaagtcg aagtagccaa accgccggct
     3901 gggctgggga ccaattggtc cccagcccag tctttcgcat caccattgtt gaatccgcgg
     3961 cgctttgtcg gcccggggcg cacgatccgt gtctcccgtt gggagcttgt agatagagga
     4021 cggctctgtt gattacggct gaccaactcc tggcccatgc cgtcggtgat tacgtcctcc
     4081 aaagcgactg gatggctacg aataagacgt cacgcacgat cgtggcgttg atccacgcgt
     4141 tgtgctacgc gattcccttc ctgctctttt cgccgtcggc cctcgggttg gcagttatca
     4201 tcggctcaca tctggtcatc gaccgcttcc gcctcgccgg ttggctgaac tggctcaaga
     4261 acggcctgcc gcgcccgcgg acaccgactg gattcccggc cgacagaccg gaatacctcg
     4321 tcgtgtggct tgtcatcatc acggacaaca tccttcatat cgcaattaac ggt
//
LOCUS       DASSGX010000253         6529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1574056, whole genome shotgun sequence.
ACCESSION   DASSGX010000253 DASSGX010000000
VERSION     DASSGX010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6529
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1574056"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="VFI12_04385"
     CDS             <1..532
                     /locus_tag="VFI12_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HET7055676.1"
                     /translation="GSTTAADNAVTALLYYMFAYTFMNIGAFAVVAWLQHRGDGIMLD
                     DFAGLAGRRPLAAAAMAIFMVSLMGIPPLLGFYAKYYIIVAAIHSDMLWLALAVVLAS
                     AVSAYFYLRVVAVMYFSPAPEKELRPFRTSLLNWGIALMVVANFGLGLFSERMVNLAD
                     KWGNALTVATQVANNR"
     gene            560..1078
                     /locus_tag="VFI12_04390"
     CDS             560..1078
                     /locus_tag="VFI12_04390"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HET7055677.1"
                     /translation="MRAAERSDARFVWDLLNEPAVQDGWGMPGTPISIHRVEQDIEQW
                     LERERVATKPAGLIIGTLDSEPIGLAIVLVDGYTGATATLSLAIDPDWQDQGYGRDAL
                     TALIDTLFDEWRVHRIEVTCEAGNERAAHLYESLGFVHEGTRRGATFLSGAYQDQRIY
                     GLLATDPRPDPA"
     gene            1075..1728
                     /locus_tag="VFI12_04395"
     CDS             1075..1728
                     /locus_tag="VFI12_04395"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET7055678.1"
                     /translation="MSAAQDPDELFDVVTWDGKPTGIQKRRADVHRDGDWHQAIHIWV
                     VGRDAHGDFLLLQRRGMDKDTAAGRLDPTVGGHLAAGETQSAVGREAQEEIGITISPT
                     DVVFAGTRRTVNEEPGVVDHELQFVYFYRCDASLDAFRPNPAEVDALVRITIDDVLLV
                     MSGQRTSAPVEVLDAVTRATGFAVATRDELSRTFDRYLYRVAIAARRFLDGEPHFSV"
     gene            1778..2062
                     /locus_tag="VFI12_04400"
     CDS             1778..2062
                     /locus_tag="VFI12_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055679.1"
                     /translation="MYVLQIEHPVPNFDVWKQAFDGDPIGREQGGVRRYRVLRPVNDP
                     HYAIIELEFEDGDKAHSFLGRLRELWSRVEGTVIMDPRARIVEMVSTTDY"
     gene            complement(2079..2873)
                     /locus_tag="VFI12_04405"
     CDS             complement(2079..2873)
                     /locus_tag="VFI12_04405"
                     /inference="COORDINATES: protein motif:HMM:NF024919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HET7055680.1"
                     /translation="MFEPGAHRISRRRLLSGAAAGAATLALGKSASASSTGITIKAPY
                     LHQGGSGANEAVNCGPTTVAMAINYSGAAAVSVASVRATLGLDGPTDIDQWAWLLDVY
                     RVPWYPTWSQEQMAGALRKGHPIVIATWMGALSAAGDYMVAWAQNSGWQGRYDSFSEG
                     HAMLIVGLSEDGYSYLVHDPNVFPGDATGFYNDGTPKGAYRRYSAWELWGTVANYANG
                     MGLAVVPYSQSFAPAKRIKRVKPEVDGIYTGPGGGLAPRRGQDQLT"
     gene            complement(2993..4531)
                     /locus_tag="VFI12_04410"
     CDS             complement(2993..4531)
                     /locus_tag="VFI12_04410"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HET7055681.1"
                     /translation="MAGASLPRYADFVVVGAGPGGCAAAAELARSGSASVLVLEAGPD
                     YGPHDSGRWPAELLEAFDLAETHGWGYDSASSYGSRVVSFSRARVVGGCSAHNGCAAI
                     WGHRLDYDGWAAAGNDGWSTDVLLPFFREANEAMRVAIPGPAHITPFHQLMLESAAAA
                     GIPLVADLNDLDEPIGMAPSPANIWNGIRWNAAFAFLDPIRNQANVRIIGDALVDTIQ
                     FHDGRVAGVTVVRDGTREFVGAGTVALAGGTYNSPAVLLRSGVGPADQLHALGIDVVA
                     DLPGVGQNLHDHPAVYLVHAGSETLKGQMASWAETHFLPEEQTIAKLRSSRCSEAFDL
                     HIYPESGPYAEGRTTWDFTIPVACMTPKARGALRIRSTDPTVPPVLDHNYLGDPDGHD
                     QAVLVEGIGIAREIARNANRYGLLGEELEPGPSIAGDHAVAGWVSDNVHHYFHAAGTC
                     AMGPDNDRAAVVTQRGAVRDVEGLFVADCSVMPVVPRANTNIPAVVVGMRIGRWLAGT
                     DTHQ"
     gene            complement(4538..>6529)
                     /gene="acnA"
                     /locus_tag="VFI12_04415"
     CDS             complement(4538..>6529)
                     /gene="acnA"
                     /locus_tag="VFI12_04415"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HET7055682.1"
                     /translation="VLLGQPLYQLMPEVVGVKLSGELQTGATATDLVLRVTEMLRGYG
                     VVGRFVEFFGKGLSNLPLADRATIANMSPEYGATTGFFPVDAETLRYLRKTGRSTDQI
                     NLVERYSKEQGLFRTDETPDPVYNAVLELDLGSIRPSVAGPRRPQDRIELENVKATFR
                     SSFNGLFSATADSDGALRGVPVKIGETISDFRHGAVAIAAITSCTNTSNPSVMLGAGL
                     LAKKAVERGLDVAPYVKTSLAPGSQVVTRYLDQSGLTGYLEALGFHTVGYGCTTCIGN
                     SGPLPGPVAEAVDDNDLIVAAVLSGNRNFEGRIHQQVRASFLASPPLVVAYALAGTVD
                     TDLTTDPIGIDPNGQPVYLADIWPTREEVEAVVAENVTSEMFQEEYATVFEGDDRWKM
                     LPVPTGELYHWEPESTYVQEPPFFIDMSPDPQPTQDIHGARVLVLVGDSVTTDHISPA
                     GAIARTSPAAQYLINHDVQQRDFNSYGSRRGNHEVMVRGTFANIRLHNLLVPGKEGNW
                     TKYLPTGEEMSIYDASYLYQREGTPLLAIAGKEYGSGSSRDWAAKGPMLLGIKAIIAE
                     SYERIHRSNLVGMGVLPLMFKTGESLKTFNLDGTETFSITGIADDLTPHKEMTVTATR
                     ADGSTVSFQAVARIDSKVEVGYYKNGGILQAVLRRLLAS"
BASE COUNT         1092 a         2146 c         2165 g         1126 t
ORIGIN      
        1 cggctcgaca accgccgccg acaatgccgt gacggccctg ctctattaca tgttcgccta
       61 caccttcatg aacattggcg cgttcgcggt ggtcgcttgg ctgcagcatc gcggcgacgg
      121 catcatgctc gacgatttcg ccggactcgc cggacgccgg ccgcttgcgg ccgcggcgat
      181 ggcgatcttc atggtttcgc tgatgggcat tccgccgctg ctcggcttct acgcgaagta
      241 ctacatcatc gtcgcggcga tccactccga catgctctgg ctggcccttg cggtcgttct
      301 tgcctctgcc gtcagcgcct acttctatct gcgggtcgtg gcggtgatgt acttcagccc
      361 ggcgccggag aaggagcttc gcccattccg gacgagcttg ctcaactggg gtatcgccct
      421 catggtggtc gccaatttcg gccttggtct cttctcggag cggatggtta acctggctga
      481 caagtggggc aacgccctga ccgttgccac gcaagtggcc aacaaccggt aagcggtgat
      541 tcgcggactg aatacaaact tgcgggcggc cgagcgatcg gacgcccgtt ttgtttggga
      601 cctgctcaac gagccggccg tgcaggatgg ctggggaatg cccggcacgc cgatttcgat
      661 ccatcgcgtc gagcaggaca tcgagcagtg gctcgagcga gagcgcgtcg cgaccaaacc
      721 ggccgggctg atcatcggga cgctggacag cgagccgatt gggctggcca tcgtcctcgt
      781 ggacgggtat accggcgcaa cggccacgct ctccctggcg atcgatccgg actggcagga
      841 ccaggggtat gggcgggacg cgctcacagc attgattgac acgctctttg acgagtggcg
      901 cgtgcaccgg atcgaggtca cctgcgaggc cggcaacgag cgggcagcgc atctctacga
      961 gtcgctcggc ttcgtccacg agggcacccg gcgcggcgcg accttcctgt ccggcgcgta
     1021 ccaggatcag cgcatctacg gcctgctcgc cactgatccc cggccggatc cggcatgagc
     1081 gccgcccagg atcccgacga gctgttcgac gtcgtgacct gggacggcaa gccgaccggc
     1141 atccagaagc gccgggccga cgttcaccgc gacggcgatt ggcatcaggc cattcacatt
     1201 tgggttgtcg gccgggacgc gcatggcgac ttcctgctcc tgcagcggcg tggcatggat
     1261 aaggacaccg ccgccggccg gctcgaccca accgttggtg gacaccttgc cgcgggagaa
     1321 acgcagtcgg ccgtcgggcg cgaggcccag gaggaaatcg ggatcacgat ctcgcccact
     1381 gacgtcgtct ttgccgggac ccgccggacc gtcaacgagg aaccaggtgt cgtcgaccac
     1441 gaattgcaat tcgtctattt ctaccggtgc gacgcgtcgc tcgacgcctt ccggccgaat
     1501 ccggccgagg tcgatgccct ggtgcggatc accatcgacg acgtgctgct ggtcatgagc
     1561 gggcagcgca cgagtgcgcc cgtcgaagtg ctcgacgccg tcacgcgcgc caccgggttc
     1621 gccgtcgcta cccgcgacga gctaagccgg accttcgacc gctatctgta ccgcgtggcc
     1681 atcgccgctc gccggttcct cgacggcgaa ccccatttca gtgtttgatt cgcgtcgaca
     1741 ccagttatcg cggcctgcct gagcgggggg agcaccgatg tacgtcttgc agatcgagca
     1801 tcccgtgccg aacttcgacg tgtggaaaca ggcgtttgac ggcgatccaa tcggacgcga
     1861 gcagggcggt gtgcgccgct atcgcgtgct gcggccggtg aacgatccgc attacgccat
     1921 catcgagctt gagttcgaag acggcgataa agcacattcc tttctcggcc gattgcgcga
     1981 actgtggagc agggtcgaag gcacggtgat catggatccg cgagcacgga tcgtcgaaat
     2041 ggtctcaacc accgactact gaacgcgcgg ccgcgaccct acgtgagctg atcctgcccg
     2101 cgccggggag ccagtccccc gccagggccg gtatagatgc cgtccacctc gggcttgacg
     2161 cgtttgatcc gcttggccgg cgcgaacgac tgcgaatagg ggacaacggc gaggcccatc
     2221 ccgttcgcgt agttcgcgac cgttccccag agctcccacg cgctgtagcg gcgatacgcg
     2281 cctttgggcg tgccgtcgtt gtagaagcct gtcgcgtcgc ccgggaacac gttcgggtcg
     2341 tgaaccaggt agctgtagcc gtcttcgctc aggccgacga tcagcatcgc gtggccctcg
     2401 gagaagctgt cataccggcc ttgccagccc gagttctggg cccatgccac catgtagtcg
     2461 ccggcagccg agagggcgcc catccaggtc gcgatcacga tcgggtggcc cttgcgcagc
     2521 gccccggcca tttgctcctg cgaccaggtc ggataccacg gcacgcggta gacatcgagg
     2581 agccacgccc attggtcgat atcggtcggc ccatcgagac cgagcgtcgc gcggacgctg
     2641 gcgacggaga cggccgccgc accgctgtag ttgatcgcca tcgcgaccgt cgtcgggccg
     2701 caattgacag cttcgttcgc gccgctgccg ccctggtgca ggtacggcgc cttgatcgtg
     2761 atgccggtag atgacgccga agcgctcttg ccgagggcga gggtggcggc gccggccgct
     2821 gctccggaca gcaggcgacg gcgcgagatg cgatgtgcgc cgggttcgaa caccgaaaag
     2881 cctccgagaa ctgcatagct ttacgcagat tcagtgtcgc acaaaatcgt cgatttctga
     2941 atccccggtc ggtctacggg tttcggggaa aatggggcgt gtccagtatg acctattggt
     3001 gagtatcggt tccagccagc cagcgtccaa tccgcatccc gacgacgacc gcggggatgt
     3061 tcgtgttcgc gcggggaacc accggcatga ccgagcaatc ggcgacgaat agcccctcga
     3121 catcccggac agcgccgcgc tgcgtcacaa ccgcggcccg gtcgttgtcc ggacccatcg
     3181 cgcaggtccc agccgcatgg aagtagtggt ggacattgtc ggagacccac ccggctaccg
     3241 cgtggtcgcc ggcgatcgat ggccccggtt ccagctcctc gccgagcagc ccataccggt
     3301 tcgcgttccg ggcgatctcc cgcgcgatcc cgatcccctc gaccaggaca gcctgatcgt
     3361 gcccgtccgg gtcgccgagg tagttgtggt cgaggaccgg cggaacggtc ggatcggtcg
     3421 agcgaatgcg aagtgcgccg cgtgcctttg gcgtcatgca ggcgacaggg atcgtgaagt
     3481 cccaggtcgt ccgcccctcg gcgtagggac cgctctccgg atagatgtgc aagtcgaacg
     3541 cctcgctgca ccggctcgag cggagcttgg cgatcgtctg ctcctccggc aggaagtgcg
     3601 tctcggccca gctcgccatc tgccccttga gtgtctcgga accggcatga acgaggtaga
     3661 ccgccgggtg atcgtgcagg ttctggccga cgcccggcaa gtccgcgacc acgtcgatgc
     3721 caagcgcgtg aagctggtcg gccgggccga ctccggatcg tagcaggaca gccggcgagt
     3781 tgtaggtccc gcccgccagc gcgaccgtgc cggccccgac aaactcccgg gtgccatcac
     3841 ggaccacggt cacgccggca acccggccgt cgtggaattg gatcgtgtca accagggcat
     3901 caccgatgat ccgcacgttc gcttgattcc ggatcggatc gaggaaggca aaggcggcgt
     3961 tccagcggat gccgttccag atgttggccg ggctcggcgc catcccgatc ggttcgtcga
     4021 ggtcgttgag atcggccacg agcggaatac cggcggcagc ggccgattcg agcatcaact
     4081 ggtggaacgg agtgatgtgc gccggcccag ggatcgcgac ccgcatcgcc tcgttcgctt
     4141 cccggaagaa gggaagcagc acgtcggtcg accagccgtc gttgccggca gcggcccagc
     4201 cgtcgtaatc gagccggtga ccccagatcg ccgcgcaccc gttatgcgcc gagcaaccgc
     4261 cgacgaccct ggcccgagag aagctgacga cgcggctgcc ataactcgac gcgctgtcgt
     4321 atccccagcc gtgcgtctcg gccagatcga acgcctcgag cagctcggcc ggccatcgcc
     4381 ccgagtcgtg cggcccatag tcgggaccgg cttcgaggac gagaacagac gccgatccgg
     4441 accgggcaag ctcggctgcc gccgcgcacc cacccggccc ggccccaacg acgacgaaat
     4501 ccgcgtatcg cggcagactc gcgccggcca cgccggccta gctcgccagc aggcggcgga
     4561 ggacggcctg caggatgccg ccgttcttgt agtaaccgac ctcgaccttg ctgtcgatcc
     4621 gtgcgaccgc ctggaaggag accgtgctgc cgtccgcccg ggtggcggtc acggtcatct
     4681 ccttgtgggg cgtcagatcg tccgcaatgc cggtgatcga gaaggtctcg gtcccgtcca
     4741 ggttgaacgt cttgagcgat tcgccggtct tgaacatcag cggcagcacg cccatcccga
     4801 ccaggttgct gcggtggatg cgctcgtaac tctcggcgat gatcgccttg atcccgagca
     4861 gcatcggccc cttcgccgcc cagtcgcgcg aggacccgga accatactcc ttgcccgcga
     4921 tcgccagcag cggggtacct tcgcgctggt agaggtagga ggcgtcgtag atgctcatct
     4981 cctcaccggt cggcaggtac ttcgtccagt tgccttcctt gcccggcacg agcaggttgt
     5041 gcaggcggat gttcgcgaag gtgccgcgaa ccattacctc gtggttgccg cgtcgcgagc
     5101 catacgagtt gaagtcccgc tgctggacgt cgtggttgat caggtactgc gcggccgggc
     5161 tcgtcctggc gatcgcgccg gccggcgaga tgtggtcggt cgtgacggaa tcaccgacga
     5221 ggacgaggac gcgcgcgccg tgaatatcct gcgtcggctg cggatccggc gacatgtcga
     5281 tgaagaaggg cggctcctgg acgtaagtcg actccggctc ccagtggtag agctcgccgg
     5341 tggggaccgg cagcatcttc cagcggtcgt cgccctcgaa gacggttgcg tactcttcct
     5401 ggaacatctc cgaggtcaca ttctccgcga cgacggcctc gacttcctcg cgggtcggcc
     5461 agatgtcagc caggtaaacc ggctggccgt tcgggtcgat gccgatcggg tcggtcgtaa
     5521 ggtcggtatc gacggtcccg gcgagggcat aagcgacgac gagcggcggc gaggccagga
     5581 acgatgcccg gacctgctga tggattcgac cttcgaagtt ccggttgccg gaaagtactg
     5641 ccgcgacgat caagtcgttg tcgtcgaccg cttcagccac cgggcccggc agcgggccgg
     5701 agttgccgat gcacgtcgtg cagccgtacc cgacggtatg gaagccaagc gcctcaaggt
     5761 acccggtcag gccggactga tcgaggtagc gcgtgactac ctgcgagccg ggcgcgagcg
     5821 atgtcttgac atatggcgcg acgtcgaggc cacgttcgac cgccttcttg gctaacaagc
     5881 cggcgccgag catgaccgag ggattcgagg tgttcgtgca gctcgtgatc gccgcaattg
     5941 caaccgcccc gtgccggaaa tcggagatgg tctcgccgat cttgaccggc acgccccgca
     6001 gcgccccatc ggagtcggcg gtcgcggaaa agagaccgtt gaacgatgag cggaatgtcg
     6061 ccttcacgtt ttcgagctcg atccgatcct gcgggcggcg cggaccggcc acgctcggcc
     6121 ggatcgagcc caggtcgagc tcgaggaccg cgttgtagac cgggtccggc gtctcgtcgg
     6181 tccggaacag gccctgctct ttggaatatc gctcgaccag gttgatctga tcggtggagc
     6241 ggccggtctt gcgcagatag cgcagcgtct cggcgtcgac cgggaaaaag ccggtcgtcg
     6301 ccccgtattc cggcgacatg ttggcgatcg tcgcgcggtc ggcgagcggg aggttgctca
     6361 agcccttgcc gaagaactcg acgaagcgac cgacgacccc gtacccgcgc agcatctcgg
     6421 tcacacggag gaccaggtcg gtcgcggtcg cgccggtctg cagctcgccg gagagcttga
     6481 cgcccacaac ttcgggcatc agctgataga gcggctggcc aaggagcac
//
LOCUS       DASSGX010000254         3287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1574138, whole genome shotgun sequence.
ACCESSION   DASSGX010000254 DASSGX010000000
VERSION     DASSGX010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1574138"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..1776)
                     /locus_tag="VFI12_04420"
     CDS             complement(115..1776)
                     /locus_tag="VFI12_04420"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7055683.1"
                     /translation="MKKPIIVAVDDDAEVLQAISHDIRREYGDRFRVVRVDSGARALD
                     VLRQVRLADETVALMVADQRMPGITGVEFLDEAKRLFPDAKRTLLTAYADTDAAIKAI
                     NDVHLDYYLMKPWDPPEDRLYPVLDDLLEDWLADYRPAFEGIRLIGHRWSAESHDVRD
                     FLARNQIPFHWLDVATDPKAQELLDLTGPGTRELPVLLFPDGAVLENPSMQAIAEKVG
                     LRHNRDLPLYDLLIVGGGPAGLAAAVYGASEGLRSAIIERHSAGGQAGMSSRIENYLG
                     FPSGLSGEDLARRATTQAQRFGADFLLAHEVKELMVNEGSVGARLTDGSEIRAHSMII
                     STGVAYRRLNEPGIAELTGRGIYYGAAITEAASIRGEEIFIVGGANSAGQAAIYFANY
                     ASKVTMLVRGESLAASMSHYLIERIEQAPNIEVRCQTAVVQADGDGHLESLVLRDSVT
                     RACERVPARSMFIFIGAVPPTDWLKDKVLLDERGFILTGPDVLATDKSAWKLDRDPLL
                     LETSVPGIFAAGDVRHGSGKRVATAVGEGAMAVMSVWQYRAQAGL"
     gene            1947..>3287
                     /locus_tag="VFI12_04425"
     CDS             1947..>3287
                     /locus_tag="VFI12_04425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HET7055684.1"
                     /translation="MQNDHHRSALGAVRQHWPFARRRADSSPGTNLPVVDPLLVESEE
                     RYRAVIENASDMIQSVRPDGTFEFVNRAWEETLGYTFDELEGKIVWDIIHPDDMEHCT
                     LLFMRAMQGETLTDVQTGFLTKDHRRIPVEGSVTSRYIDGQIVATHAFFRDITERLRT
                     QELEEQNARLEQEQHERYLEKMAALGKLSAGLSHELNNPAAAAQRASARLAESLSERD
                     AAARELTVCGLQKPQWDVLEAVVVASMDHAQDALEPLEASQREETIEQWLEEQDVANA
                     WTLAPTLARAGVGEAELDRLTEVFPIEQLPAALRWISGAVAIRDEAEIIGRSTHRISE
                     LIGAVKAYSFMDRAVEQEVDVHDGLENTLTMLAHKLRNVTIKREYDRGLPKIRAFGSG
                     LNQVWTNVIDNAIDATNGQGTVTIRTRRDGDRVVVEVADNGCGIPEDDLRRVFEP"
BASE COUNT          569 a         1034 c         1145 g          539 t
ORIGIN      
        1 gcagcgacgg gtttgccccc gcaaacccct acaattccgc gcctccgcgc ctccgcgcct
       61 ccgcgcctcc gcgcctccgc gcctccgcgc ctccgcgcct ccgcgcctcc gcgcctacaa
      121 ccccgcctgc gcccggtatt gccagaccga catgacggcc atggcgccct cgccgacggc
      181 ggtggcgaca cgcttgccgg agccatggcg gacgtcgccg gccgcgaaga taccgggcac
      241 gctggtctcg agcaacagcg ggtcgcggtc gagcttccag gcgctcttgt cggtggcgag
      301 tacgtcgggg ccggtcagga taaagccgcg ctcgtcgagc agcaccttgt ccttcagcca
      361 atcggtcggg gggacggcgc cgatgaaaat gaacatggac cgggccggca cgcgctcgca
      421 cgcgcgggtc actgaatcgc gaagcacgag tgattcgaga tggccgtcgc cgtctgcctg
      481 cacgactgcg gtttggcagc ggacctcgat gttcggcgcc tgctcgatcc gctcgatgag
      541 gtagtgagac atgctggcgg cgagcgattc cccccgcacc agcatcgtga ccttgctggc
      601 gtagttggcg aaatagatcg cggcttgccc ggccgaattt gccccgccga cgatgaagat
      661 ctcctcgccg cggatcgagg cggcctcggt gatcgccgcg ccgtagtaga tgccacgccc
      721 ggtcagctcg gcgatgcccg gctcgttgag gcggcgatac gcgacaccgg tcgagatgat
      781 catggaatgg gcgcggatct ccgagccgtc cgtcaacctg gcgccgacgg agccttcgtt
      841 caccatcagc tccttgacct cgtgcgccag caggaagtcg gcgccaaacc gctgggcttg
      901 cgtggtcgcg cgccgggcaa ggtcctcgcc cgagaggccc gaggggaagc cgagatagtt
      961 ctcgattcgc gagctcatcc ccgcctgccc gccggcggaa tgtcgctcga tgatggcgct
     1021 ccgcagccct tcggacgccc catagacagc cgccgcgagg ccggctggcc cgccgccgac
     1081 gatcagcagg tcgtagagcg gaagatcgcg gttgtggcgg agcccgacct tctcagcgat
     1141 cgcttgcatc gacgggttct cgaggaccgc gccgtccgga aagagcagga ccggcagctc
     1201 ccgcgtgccg gggccggtca gatcgagcag ctcctgggct ttcgggtcgg tcgcgacgtc
     1261 gagccagtgg aaggggatct ggttgcgggc gaggaaatcg cgcacgtcgt gggattcggc
     1321 cgaccagcga tggccgatca ggcgaatgcc ctcgaaagcc ggacgatagt cggccagcca
     1381 gtcctcgagc aagtcgtcca ggaccgggta gaggcgatcc tcgggcggat cccacggctt
     1441 catcaggtag taatcgaggt ggacgtcgtt gatcgccttg attgcggcat cggtatcggc
     1501 gtaggcggtc aggagggtgc gcttggcgtc ggggaagaga cgcttggcct catcgaggaa
     1561 ctcgacgccg gtgatgccgg gcatgcgctg gtcggcgacc atcagggcga ccgtctcatc
     1621 ggccagccgg acctggcgga ggacatcgag cgcgcgggcg ccggagtcga cgcggacgac
     1681 ccggaagcga tcgccgtact cgcggcggat gtcgtgcgag atcgcctgaa gcacttcggc
     1741 gtcgtcgtcg acggccacaa tgatcggctt cttcatcggg attctcctga cggctcccgc
     1801 tggtggccga tcgagccggg ctgcggggta tttcgttatc ggcggaaaga atttgactcc
     1861 tgatacgata gtactgcatc agctactgcc cataaagggg gtcctctgtg cgcgacgagc
     1921 catttgtcag cagtcctcac tttgcagtgc agaacgatca ccatcgctct gcgcttggcg
     1981 cggtgcgcca gcactggccg tttgcgcggc gacgggccga ttccagccca ggcaccaatc
     2041 tgccggtggt cgatccgctc cttgtcgaga gcgaggagcg gtaccgggcc gtcatcgaga
     2101 acgcctcgga catgatccag agtgtgcgtc cggacgggac gttcgagttc gtcaaccggg
     2161 cttgggaaga gacgctgggc tacacatttg atgagctcga aggcaagatc gtctgggaca
     2221 tcattcaccc ggatgatatg gagcattgca cgctgctgtt catgcgagcc atgcagggcg
     2281 agacgctcac cgacgtccag accggtttcc tgaccaagga tcatcggcgg attcccgttg
     2341 agggcagcgt gacctcgcgg tatatcgacg gccaaattgt tgccacgcac gccttctttc
     2401 gggacatcac cgagcggttg cggacgcagg agctggagga gcagaacgcg cgactggagc
     2461 aggagcagca tgagcgctac ctcgagaaga tggccgcgct cggcaagctc tcggcgggcc
     2521 tgtcgcacga gttgaacaat ccggccgcag cagcgcagcg ggccagcgcc cgcctggccg
     2581 agagcctgag cgagcgcgat gcggccgccc gggagctgac cgtttgcggg ttgcagaagc
     2641 cacagtggga cgtgcttgag gcggtcgtcg tggcgtcgat ggatcacgcc caggatgcgc
     2701 ttgagcccct ggaagcaagc cagcgcgagg aaacgatcga gcaatggctg gaggagcagg
     2761 atgtcgcgaa tgcctggaca ctggcgccga cgctggcgcg ggcgggcgtc ggcgaggccg
     2821 agctcgaccg gttgacggaa gtctttccga tcgagcagtt gcctgctgcc ctgcgctgga
     2881 tcagcggtgc ggtcgcgatc cgggatgagg cggagatcat cgggcgaagc acgcaccgta
     2941 tctcggagct gatcggggcg gtcaaggcgt attcgttcat ggaccgggcg gtggagcagg
     3001 aggtcgatgt ccatgatggc ctcgagaaca cgctcaccat gctggcgcac aagctccgga
     3061 acgtgacaat caagcgcgag tacgaccgtg gcctcccgaa gatccgcgcc ttcggcagcg
     3121 ggctgaacca ggtctggacc aacgtcatcg ataacgcaat cgacgcgacg aatggccagg
     3181 gcacggtcac gatccggacc cggcgagacg gcgatcgggt ggtggtcgag gtggcggata
     3241 acggctgcgg gatcccggag gacgatctgc gccgggtctt cgagccc
//
LOCUS       DASSGX010000255         4114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_483249, whole genome shotgun sequence.
ACCESSION   DASSGX010000255 DASSGX010000000
VERSION     DASSGX010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483249"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /locus_tag="VFI12_04430"
     CDS             <1..1155
                     /locus_tag="VFI12_04430"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7055685.1"
                     /translation="ISPEAFDIDASFFFVTTVILGGLGTIAGPIVGSLILTYLPEWLQ
                     RFAEFRLIIYGSLIVFSLYFMPHGLVGAVSDWFRALLTRVGLGGLFTKGAYAAPLDSV
                     TARSSRLPPRPAPRLMPESARSPGAEPLLELNVVSRAFGGLVAVHDVDLRVEPETIHA
                     LIGPNGAGKTVLLNSICGYYPPTKGEIRFAGRRINGLSPNAISRLGIARTFQTTQLFP
                     EMTVIDNVMSGTYAASRTGYVDAFLQSPRLRHEKRHMQRRALELLAFVGFVDDPYAIA
                     GGMPFGVQRLIEIARALALNPWLLIMDEPAAGLNPLEVERLTSLITAIRDSGTAVLLV
                     EHHMDLVMGISDVVTVLDYGEKIAEGIPLHVQQTPRVIEAYLGTVELVDA"
     gene            1148..1864
                     /locus_tag="VFI12_04435"
     CDS             1148..1864
                     /locus_tag="VFI12_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7055686.1"
                     /translation="MLELVDLHVRYGAIEALRGASLIVHPGEVVTVIGSNGAGKSTML
                     KAIAGLLRPSRGRIRLNGQEIAGKPAHQIVPLGISLVPEGRKIFADQTVWDNLLLGGY
                     VRRREDLRERADAQLSRFTVLRERRHQQAGALSGGQQQMLAIARGLMARPKLLLLDEP
                     SIGLAPRLVREVFAIVEELRQEGMTILLVEQMAAMALAIADRAYVLEQGAIVLEGSGQ
                     ELLASPAVRSAYLGSSARLA"
     gene            complement(2395..3393)
                     /locus_tag="VFI12_04440"
     CDS             complement(2395..3393)
                     /locus_tag="VFI12_04440"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HET7055687.1"
                     /translation="MTEPAIITPYGGPIVASLRYRREFLQERQVLLRFHLPRFRAGHV
                     AGIVIPVDGYDDLALLLTEISESEGQLRLARAPSEARAIMADGAVAVLLGVTFEAIGA
                     KPDILHVYQELGLSVFPLALNPRNLLTDGCGERTGAGLSYLGIDVVRKVATCGGLVDI
                     SHTSEAGFEDVAAHLSGPFIASHSNARTLCDNPRNLTDDQARTIAAHGGVVALSTFPT
                     LVSTAPVATLDHFLDQIDYLVQLIGAESVAIGADFIDDVRDLYVPKIQRTDPGGAMYR
                     GATVTTTQGLETIADLRHVAAALPSRGYSAPECERILGGNFLRVWEDARRRAGLPA"
     gene            complement(3431..>4114)
                     /locus_tag="VFI12_04445"
     CDS             complement(3431..>4114)
                     /locus_tag="VFI12_04445"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HET7055688.1"
                     /translation="MLPMTAAHSALLIIDVQYLDAHPDYGTLAHARELGLSDSYTYYT
                     ERLQDLVIPNIQRLQASFRQHGMEVVHVHIESLTTSGRERGWLYRTLAIGAPRGSREA
                     EILPEVAPVGDELVIAKTSSSPFNSTNIDVMLRNLGIEQLVLVGVMTSGCVESTARDA
                     ADRGYDVILVTDACAARTPEMEQKTHEAIGGTFVHLLTTDEVLDLVGDVAHGGDTNEK
                     GARQTALAR"
BASE COUNT          684 a         1360 c         1329 g          741 t
ORIGIN      
        1 atcagccccg aagcgttcga catcgacgcc tcgttcttct tcgtcaccac cgtcattctg
       61 ggcgggctgg ggacgatcgc gggaccgatc gtcggctccc tgatcctgac gtatctcccg
      121 gagtggctgc agcgctttgc cgagttccgg ctgatcatct acgggagcct gatcgtcttc
      181 tcgctctact tcatgccgca tggtctcgtc ggcgccgtct cggattggtt ccgcgccctc
      241 ctgacgagag tggggctggg aggcttgttc acgaagggcg cctatgccgc ccccctcgac
      301 tcggtcacgg cacgctcctc gcggctacca ccgagacccg cgccgcggct catgcccgag
      361 agcgcacgct ctccgggggc cgagccgctg ctcgagctga atgtcgtgtc tcgcgccttc
      421 ggtggtctcg tcgccgtgca tgatgtcgac ctccgggtcg agccggagac gatccacgcc
      481 ctgatcggcc cgaacggcgc gggcaagacc gtcctcctca attcgatctg cggctactac
      541 ccgccaacca agggtgaaat ccgcttcgcg ggaaggcgca tcaacggcct gtctcccaat
      601 gcgatctcgc gcttggggat cgcccggacg tttcagacaa cccagctctt tccggagatg
      661 accgtcatcg acaacgtgat gagcgggacg tacgccgcga gccgaacggg ctatgtcgac
      721 gcctttctgc agtcgccacg gctacggcat gagaagcgac acatgcagag gcgggcgctc
      781 gaactgcttg catttgtcgg attcgtcgat gatccctacg ccatcgctgg cggcatgccc
      841 ttcggcgtgc agcgactcat cgaaatcgcg cgcgcccttg ccctgaaccc gtggctgctg
      901 atcatggacg agccggccgc cggattgaac ccgctggagg tcgagcgact cacctcgctg
      961 atcaccgcta tccgcgactc ggggacagcc gtcctgctcg tcgagcacca catggatctg
     1021 gtgatgggca tctcggacgt ggtgaccgtc ctggattatg gcgagaagat cgccgaggga
     1081 atcccgctgc acgttcagca gaccccccgg gtcatcgagg cgtatctcgg gacggtggag
     1141 ctggtcgatg cttgagctcg tcgatctcca tgtccgctac ggggcgatcg aggcgcttcg
     1201 cggcgcctcg ctgatcgtgc atccaggcga ggtggtgacc gtcatcggca gcaatggcgc
     1261 gggcaagagc acgatgttga aggcgatcgc cggtctgctg cgcccctccc gaggacgcat
     1321 tcggctgaac ggtcaggaga tcgcggggaa gcccgcgcat cagatcgtgc cgctgggcat
     1381 ctccctggtg ccagaggggc ggaagatctt cgccgatcag accgtctggg ataatctgct
     1441 gctcggtggc tacgtccgtc gccgcgagga tctgcgggag cgcgccgatg cccagctctc
     1501 ccggtttacg gtgctccgcg agcgtcgcca tcagcaggcc ggtgcgctga gcggcggaca
     1561 gcagcagatg ctggcgatcg cccgcgggct gatggcccgg cccaagctcc tcttgttgga
     1621 tgaaccttcc atcggcctcg ctccccggct ggtgcgggag gtcttcgcca tcgtcgagga
     1681 gctgcgccag gaagggatga cgatcctgct tgtggagcag atggcggcga tggcactcgc
     1741 gatcgccgac cgggcgtacg tactcgagca gggggcgatc gtgctcgagg gttcgggcca
     1801 agagcttctg gcttctcccg cggtccgctc ggcgtacctc gggtcatcgg cgcggctcgc
     1861 ctgattgacc tcgccgctcc atccgtggcc cgcagttcaa acggctgact cagtctcgtc
     1921 atatggagac ccgatcttgg ccggaatcgg cgcgtctgtg gacctgaagg cgccgcaagc
     1981 gcgccgcata ccgcgtcgtg atgcatcagt cgccgtagtg ggactctccc gtacccccca
     2041 gcctcacgca cgagaccgtg tgcccggccc ggagagcggt cacgactgcg cccacgcgaa
     2101 gatgccatgc ccggatgctg gtcttccacg ccatgtcggt gtaagcacgg gcaggctgac
     2161 gacacctgaa tccgtgccaa ttggaggtcg ctgagacatg gcgtgaatcc ggcgacgaca
     2221 gtcaaccagg atcgaggcga tcacgggcca ggtaaaggca tcagccgctc cgctccgggc
     2281 gagtggtaat tgcacacgtt cagggccgag tggtcaacgt gtccgagcac cgcgacacac
     2341 cggcaagcag cccaccatcc tcaggcagcc cggctccact gcttcgggcc accatcaggc
     2401 aggcaaaccg gctcggcggc gggcgtcttc ccagacacgc aagaagttgc caccgaggat
     2461 gcgttcgcac tccggtgcgc tatatccccg gctcgggagg gccgccgcaa catggcggag
     2521 atccgcgatg gtctccagcc cctgcgtcgt cgtgaccgta gccccgcggt acatggctcc
     2581 ccccgggtcg gtccgctgga tcttgggcac gtagagatcg cggacatcgt cgatgaagtc
     2641 cgcgccgatt gcgacggatt cggcaccgat gagctggacg aggtagtcaa tctgatccag
     2701 gaaatggtcg agtgtcgcca ccggcgccgt cgagacgagc gtgggaaagg tgctgagagc
     2761 caccaccccg ccatgcgccg cgatcgttcg cgcctgatcg tctgtgagat tgcgcggatt
     2821 gtcgcacagc gtgcgggcgt ttgagtgcga cgcgatgaat gggccggaca ggtgcgctgc
     2881 cacatcctcg aaccccgctt ccgacgtgtg ggagatgtcg acgagccccc cgcaggtggc
     2941 gactttgcgc acgacgtcga tccccaggta actcaagccg gccccagtac gctcgccgca
     3001 gccatcagtc agcaggttgc gcggattcag cgccagcggg aacaccgaca gccccagctc
     3061 ttggtagacg tgcaggatgt cgggcttcgc gccgattgcc tcgaaggtca ccccgagcag
     3121 gaccgccacc gccccgtcgg ccatgatggc ccgcgcctcg ctcggcgcgc gggccaggcg
     3181 aagctgccct tccgactcgc tgatctcagt cagcaggaga gcaaggtcgt cgtatccgtc
     3241 gacggggatg acaataccgg cgacgtggcc ggcccggaag cgcggcagat gaaaccgcag
     3301 caggacttga cgctcctgca ggaattcgcg acgatagcgc aggctggcca caatcggacc
     3361 cccataggga gtgatgatcg ccggttccgt catatcgctc ctcggagtct gccgtcctgc
     3421 ctgtgcgcac tcagcgcgca agcgctgttt gccgcgcacc cttctcgttg gtatcgcccc
     3481 cgtgtgcgac gtcgccgacc agatcgagaa cctcgtcggt ggtcaacagg tggacaaatg
     3541 ttccaccgat cgcttcgtgc gtcttctgct ccatttccgg ggtccgggct gcgcaggcat
     3601 cggtcaccag gatgacgtca tagccacggt ccgcggcgtc gcgcgccgtg gactcgacac
     3661 agccgctggt catgaccccg accaggacaa gttgctcgat cccgaggttg cgcagcatga
     3721 cgtcgatatt cgtcgagttg aaggggctgg aggatgtctt ggcaatcacc aactcgtccc
     3781 caaccggcgc cacctctggc aagatctcgg cttcgcgcga tcctcgcggc gcgccgatgg
     3841 ccagcgtgcg gtagagccac ccgcgttctc gcccgctcgt tgtgagcgat tcgatatgga
     3901 catggacgac ctccatgccg tgttgccgaa agctcgcttg cagccgctga atattcggga
     3961 tcacgagatc ctgaagccgc tccgtgtagt acgtgtacga atcgctgagc cccagctcgc
     4021 gcgcgtgcgc gagcgtgccg tagtctgggt gcgcgtcgag atactgcacg tcgatgatca
     4081 ggagcgcgct gtgcgcggct gtcatcggca acat
//
LOCUS       DASSGX010000256         4623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_593084, whole genome shotgun sequence.
ACCESSION   DASSGX010000256 DASSGX010000000
VERSION     DASSGX010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4623
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593084"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..348)
                     /locus_tag="VFI12_04450"
     CDS             complement(25..348)
                     /locus_tag="VFI12_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055689.1"
                     /translation="MGTDRVLSGMPDGRLYRVVAGEVHSFRTLLIEDQLGERFLLDTN
                     RRTLEPISDGRAGELLSARTFRPWNGPARWATVTDLTPVEEWTSRQMDLPPEPDEITS
                     DSLNQ"
     gene            complement(412..3018)
                     /gene="lon"
                     /locus_tag="VFI12_04455"
     CDS             complement(412..3018)
                     /gene="lon"
                     /locus_tag="VFI12_04455"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HET7055690.1"
                     /translation="MPDHIETDAAEPGAPPVEETVASAPKRQRKPRERKRTKAEQRPA
                     GLILPTLIVDDMVVLPNLTIPFPVEDDETAYVFERACRNSKRQVFLVCERPIRLPEAP
                     TTQESATDPAILKEFFSSLDRDQLDETSDGWASEPIQMPAADYEVCPVGIIAEVGQFA
                     PTVGRVPQVMLQGISRGIVLNVVQDEPMMVCEVMPHEDQVPPKALSEAPMAALIEQIE
                     NYVSMHPGAPEDVLAMLEGIQEPGHLADLVAISPEFTPLQRQQVVESLDPLERLRTVS
                     VIVEKRLMVLSLRNAIVADAQNGLDKQQREYFLREQIRSIQKELGEGTAEETLAKDLR
                     QRVEEAGMPEEIKAKTLDQVDRLEQQHPFSPEIGVIRSYVEWLIELPWSKETEDLLDL
                     DHAARILNEDHYGIQKVKDRIIEYIAVRKLAGDKVRAPIICFVGPPGVGKTSLGRSIA
                     RAIGRNYVRMSLGGVRDEAEIRGHRRTYVGAMPGRVIKALRDAKSNNPVIVLDEIDKV
                     GSDAFRGDPSSALLEVLDPEQNSTFSDHFLEVPFDLSKVIFITTANLLEPIPPALRDR
                     MEVIEVSGYTEAEKLAIARQYLMPKALESHGLTPELLEITDDALMQIIRVYTEESGVR
                     NLEREIGSVCRKAARSIAGQEEPERLTIDADDLIDVLGVPRYEYGLAEEQDEIGVATG
                     AAVTSVGGDLLSIEVTIMEGKGELILTGQLGEVMQESGRAAISYARSHAADFGIAPGF
                     FDKHNIHVHIPAGAIPKDGPSAGITMATALISALTGRKVRKDVAMTGEMTLRGKVLPI
                     GGLKEKTLAAHRGGIQTFILPQRNAKDLAELPDLVKKELHLIQVANIAEVLDVALLPA
                     PSVQFRVAESVA"
     gene            3156..3713
                     /locus_tag="VFI12_04460"
     CDS             3156..3713
                     /locus_tag="VFI12_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055691.1"
                     /translation="MANFGELREAVLPEIAAVLERVDADAWETFCTTIAGAHRVVCFG
                     MGREGLALRGFCMRLAHMGFDAHMAGDVTAMPVGSGDVVLVTSGPGNLTMTWSMIELA
                     HRAEATVVVVSAQPGAPDPQAADFALTIPAQTMANDRGSASVLAMGTAFEIAMGIVFD
                     LAVIRIQELRGQTLEQMRARHFNLE"
     gene            complement(3715..4353)
                     /gene="pdxH"
                     /locus_tag="VFI12_04465"
     CDS             complement(3715..4353)
                     /gene="pdxH"
                     /locus_tag="VFI12_04465"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HET7055692.1"
                     /translation="MSLQDLRKDYTLAGLLESDLDPDPIAQFRTWFAEAQAAKGFEPN
                     AMTVATADADGNPGARTLLLKGLDERGFVFYTNYESGKGRQLESNPRAELLFYWPEVE
                     RQVRVHGAVERLDRTASEQYFHSRPLGNQLGAVVSQQSTVVPNRDALEEAYAALEVTY
                     ATEPVPMPEYWGGYRVVPEWIEFWQGRRSRLHDRLRYRRSDDGAWVVERLAP"
     gene            complement(4360..>4623)
                     /locus_tag="VFI12_04470"
     CDS             complement(4360..>4623)
                     /locus_tag="VFI12_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055693.1"
                     /translation="ACVLLSTHDVELAAALADRVVLLGHGEVIADGTPAGVLSGSMAF
                     SSQINRLFGPGYLTLADLDLTDTISFGDTSGRLVDSPAIAASG"
BASE COUNT          752 a         1513 c         1511 g          847 t
ORIGIN      
        1 tgtccggccg ggctcgtgtt tcgattactg gttgagggaa tcggacgtta tctcgtccgg
       61 ttcaggcggg aggtccattt gccgcgaggt ccactcctcg accggcgtga gatcggtcac
      121 cgttgcccag cgcgcggggc cgttccaggg ccggaaggtg cgggcggaga gcagctcgcc
      181 ggctcggcca tccgatatcg gttccagtgt cctccggttc gtatccagca agaatcgctc
      241 cccgagctga tcctcgatca gaagtgtccg gaagctatgc acttcgccgg caacgacccg
      301 gtagagccgt ccgtccggca tgcccgacag aactcgatcg gtccccataa tccgcgctcg
      361 cttgtttcgt gcgatccccc actgacactc tgcggcacaa cctgcccggc gtcaagcgac
      421 cgattcggcg acccggaatt gcaccgacgg cgcggggagc agcgccacat ccagcacctc
      481 ggcgatgttc gcgacctgga tcaggtgcag ctctttcttg accaggtcgg gcaactcggc
      541 caggtccttg gcattacgct gcggcaggat aaaggtctgg attccgccgc ggtgtgccgc
      601 gagcgtcttt tccttcagcc cgccgatcgg caacaccttg ccgcgcagcg tcatctcgcc
      661 ggtcatcgcg acatccttac gcaccttgcg gccggtcagg gccgagatca gcgcggtcgc
      721 catcgtgatg ccggcggatg gcccgtcctt cgggatcgcg ccggccggga tgtggacgtg
      781 gatgttgtgc ttgtcgaaga agccgggcgc aatcccgaaa tcggccgcgt ggctgcgcgc
      841 gtagctgatc gccgcccgac ccgactcctg catgacttcg ccgagctgac cggtcaggat
      901 cagttcgccc ttgccctcca tgatcgtgac ctcgatcgag agcaggtcgc cgccgacact
      961 ggtgacggcc gcgccggtcg cgactccgat ctcgtcctgc tcttcggcca ggccgtattc
     1021 gtagcgaggc acgccgagaa catcgatcag gtcgtcggca tcgatcgtga gccgctccgg
     1081 ctcctcctgt ccggcgatcg agcgggccgc cttccggcag accgaaccga tctcccgctc
     1141 aagattccga acgcccgact cctcggtgta gacgcgaatg atctgcatca gcgcgtcgtc
     1201 ggtgatctcg agcagctccg gcgtcaggcc gtgcgactcg agcgccttcg gcatcaggta
     1261 ttgcctggcg atcgccaact tctcggcctc ggtgtagccg gagacctcga tcacctccat
     1321 ccggtcccgc aatgcgggcg ggatgggctc gagcaggttg gcggtcgtga tgaagattac
     1381 cttcgagagg tcgaacggca cctcgaggaa gtggtcggag aaggtcgagt tctgctccgg
     1441 atcgagcact tcgagcaggg ccgacgacgg atcgccgcgg aaggcgtccg agccgacctt
     1501 gtcgatctcg tccagcacga tcaccggatt gttgctcttg gcgtcgcgca gggccttgat
     1561 cacgcggccg ggcatcgcgc cgacgtaggt gcggcggtgg ccccggatct cggcttcatc
     1621 ccgcacgccg ccgagcgaca tccggacgta gttgcgcccg attgcgcggg cgatcgaacg
     1681 gccgaggctc gtcttgccaa cgccgggcgg gccgacgaag cagatgatcg gcgcgcggac
     1741 cttgtcgccg gccagcttgc ggaccgcgat gtactcgatg atccggtcct tgaccttctg
     1801 gatgccgtag tgatcctcat tgaggatccg ggccgcatga tcgaggtcga gcaaatcctc
     1861 ggtctccttg gaccacggta gctcgatcag ccattcgacg tagctgcgaa taacgccgat
     1921 ctcgggcgag aagggatgct gctgctcgag gcgatcgacc tggtcgagcg tcttggcctt
     1981 gatctcctcg ggcatgccgg cctcttcgac ccgttgtcgc aaatccttgg cgagcgtctc
     2041 ttcggccgtg ccttcgccga gctccttctg gatcgagcga atctgctcgc gcaggaagta
     2101 ctcgcgctgt tgcttgtcga ggccgttttg cgcatcggcc acgatcgcgt tgcgcagtga
     2161 gagcaccatc agccgcttct cgacgatcac actgaccgtc cgcaggcgct ccagcggatc
     2221 gagcgattcg acgacctgct gacgctggag cggagtgaac tcgggcgaga tcgcgaccag
     2281 gtcggccagg tggccgggtt cctggatgcc ctcgagcatg gccagcacgt cttccggcgc
     2341 gccggggtgc atgctgacgt agttctcgat ctgctcgatc aaggcggcca tcggcgcctc
     2401 gctgagcgcc ttcggcggga cctgatcctc gtgcggcatg acttcgcaga ccatcatcgg
     2461 ctcgtcctgg acgacattga gcacgatccc gcgcgagatg ccctgcagca tcacctgcgg
     2521 cacgcgcccg accgtcggcg cgaactgacc gacctcggcg atgatcccaa ccggacagac
     2581 ctcgtagtcg gctgccggca tctggatcgg ctcgcttgcc cagccgtcac tggtttcgtc
     2641 gagctgatcg cgatcgagtg acgagaagaa ctccttgaga atggcggggt ccgtcgcgct
     2701 ctcctgggtt gtcggcgctt cgggcagccg gatcggccgc tcgcagacga ggaagacctg
     2761 ccgcttgctg ttccggcagg cccgctcgaa gacgtatgcc gtctcgtcgt cctcgaccgg
     2821 aaagggaatg gtcaggttcg gcagcaccac catgtcgtcg acgatcaatg tgggcaggat
     2881 caggccggct ggccgctgct ctgccttggt ccgcttccgc tcgcgcggtt tgcgttgtcg
     2941 tttcggagcc gaagcgaccg tctcttcgac gggcggagcg cctggctcgg cagcgtcagt
     3001 ctctatatgg tcaggcaaaa gataatcctc tggataacgc acggccgggg cgccgcgcgt
     3061 gggtgctatc cccatattct aagctgttgg cggcacaaac gacggtttcg cgccacctcc
     3121 catacgggcg gggcatgatg gagagcgaat accaaatggc gaattttggg gagcttcgtg
     3181 aggcagtact tccagagatt gccgccgttc tggagcgagt ggatgcggat gcctgggaga
     3241 ccttctgcac gacgatcgcc ggcgcgcacc gggtcgtctg cttcggaatg ggccgcgaag
     3301 ggctcgccct gcgcggcttc tgcatgcggc tggcgcacat gggcttcgac gcccacatgg
     3361 ccggcgatgt cacggcaatg ccagtcggat cgggcgatgt agttctggtc acctccggcc
     3421 cgggcaattt gacgatgacc tggagcatga tcgaactcgc gcaccgggcg gaggcaacgg
     3481 tcgtcgtggt gtcggcccaa ccgggcgcgc ccgatccgca agccgccgat tttgccctca
     3541 cgattcccgc gcaaacgatg gcgaacgaca gaggcagcgc gagcgtgctg gcgatgggaa
     3601 ctgccttcga gatcgcgatg gggatcgtgt tcgacctggc cgtgatccgg attcaggagc
     3661 tgcgcgggca gacgctcgag cagatgcgcg cccggcactt caacctggaa tgagctacgg
     3721 ggcgagccgc tctacgaccc atgcgccgtc gtccgaccgc cggtaacgca ggcgatcgtg
     3781 caggcggctg cgacgccctt gccagaactc gatccactcc ggcacgaccc ggtatccccc
     3841 ccagtactcc ggcatcggca ccggctcagt cgcgtaggtc acttccagcg cggcgtatgc
     3901 ctcctcaagt gcgtcccggt tcgggacgac cgtgctctgc tgcgagacga cggccccgag
     3961 ctggttgccg agcgggcggc tgtggaaata ctgctccgac gcggttcggt ccagccgctc
     4021 gacggcgccg tggacccgga cctgccgctc gacctccggc caatagaaga gcagctcggc
     4081 gcgtgggttg gactcgagct ggcgaccctt cccgctttcg taattggtgt agaaaacgaa
     4141 gccgcgctca tccagcccct tgagcaggag tgtccgcgcg ccgggattcc cgtcggcatc
     4201 ggcggtcgcc acagtcatcg cattcggctc gaaccctttg gccgcctgcg cctcggcgaa
     4261 ccaggtccgg aactgggcga tcggatccgg atcgaggtca gattcgagca acccggcgag
     4321 cgtgtaatcc ttgcgaagat cctgaagcga catctcggtt caccctgacg cggcaattgc
     4381 cggcgaatct accagcctgc cggaagtatc cccgaacgag atcgtgtccg tgaggtcgag
     4441 atcggcgagg gtcaggtagc cgggaccgaa gagccggttg atctgcgacg agaaggccat
     4501 cgagccggag agcaccccgg ccggagtccc gtccgcgatc acctcaccgt ggccgagcag
     4561 caccactcga tcggcgagcg cggccgccag ctcgacgtcg tgcgtcgaga ggaggacgca
     4621 ggc
//
LOCUS       DASSGX010000257         6893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1029614, whole genome shotgun sequence.
ACCESSION   DASSGX010000257 DASSGX010000000
VERSION     DASSGX010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6893
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1029614"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1307
                     /gene="ftsH"
                     /locus_tag="VFI12_04475"
     CDS             <1..1307
                     /gene="ftsH"
                     /locus_tag="VFI12_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET7055694.1"
                     /translation="LARAVAGEAGVPFFSISGSEFVEMFVGVGASRVRDLFDQAKRNS
                     PCIVFVDEIDAVGRQRGAGLGGSHDEREQTLNQILVEMDGFDTNTNVIVVAATNRPDV
                     LDPALLRPGRFDRQVILDRPDMKGRTAILKVHTKGKPLDKTVSVEEVARQSPGFSGAD
                     LANLVNEAAILAARRNKKSVGMSEFNEAIDRVIAGPERRSRLITEEEKEIIAYHEGGH
                     AVVQRVLPKCDPVTKVTIVSRGMALGYTMSLPQEDRYLHSKSEFEDKIAGMLGGNVSE
                     QIVFGDTTTGSSNDIEKATNLARAMVTQYGMSEKLGPLTFGKKEEMIFLGREISEQRN
                     YSDEIAAKIDGEVREIIDRAYERAKEALTRHRAVLDKLAELLIEKETIEADEFESLFE
                     GILPPRGTAKPLLPEGEAEPEAEPTGTDEAPARRRGPAPQPA"
     gene            1657..2739
                     /locus_tag="VFI12_04480"
     CDS             1657..2739
                     /locus_tag="VFI12_04480"
                     /inference="COORDINATES: protein motif:HMM:NF015736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HET7055695.1"
                     /translation="MIDEPIAKSTPAATNWQLEVGEGTLAPRSALDVLTNLNERIASG
                     NIDEFQPIATGFVPLDKVIGGGLRPGELMLIGGAQGQGKTTMALQMARNAAASGQATA
                     LYVCFEHEEEYLIQRMIAMESALSHLPKKSGAVKIQDVKKEVLGSYTASAEAGTRAKL
                     GTNPRLRPSLERIARYGSSLFLLRGTATHSTIENLRALVQEHRGDDDRRLILFVDYLQ
                     KVPVFPEPADEAEKVTRIVSGLKDIALACEVPVVAIVAADREGLKAKRLRNHHLRGSS
                     ALNYESDIILILNDKYNIVAKVNIEFNPYQAQRFRDWVVATIEKNRGGQDNIDLEYEK
                     LFEYSCFDPTGRQTSEKLIEERLYND"
     gene            complement(2749..3135)
                     /locus_tag="VFI12_04485"
     CDS             complement(2749..3135)
                     /locus_tag="VFI12_04485"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HET7055696.1"
                     /translation="MLTTDPHKVLQILSSAEPEVVQAAYRALASKYHPDRDGSSYAAR
                     RMREINEAYAQLRAPAARPRSLAERVIPKEKSSRLSFGRYAGWSLGDLARKDPDYLRW
                     LSRTASGIGYRTEIYQILGGMTSTAA"
     gene            3298..4038
                     /locus_tag="VFI12_04490"
     CDS             3298..4038
                     /locus_tag="VFI12_04490"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET7055697.1"
                     /translation="MSSLQAVFLDIGDTVMRPNPSWEHVYAAAFAEFGVEVTPEQLLP
                     ALRKAYHHGGWGFAGGFEPTIETSFRRTVEIDQAAIVDLGLGPMPDAFFQRLSELFTL
                     TSNWHVFPDAPGVLDGLHVRGLTVGAVSNWVWSLPELLHSLELMSKFDFIAVSSRIGF
                     EKPHPAIFRWALEQADAAADAVIHVGDHLDADVRGAEAVGISGVLIDRVGRYAPDDLP
                     QDVTVIHSLDELLPIVDERLAEADRLAG"
     gene            4035..4613
                     /gene="thpR"
                     /locus_tag="VFI12_04495"
     CDS             4035..4613
                     /gene="thpR"
                     /locus_tag="VFI12_04495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HET7055698.1"
                     /translation="MSETWRCFIAVPIAQSLRKDLATMVAALGDDPVMADFRWTDPAG
                     WHLTVAFLGATPPSDVDRIAGLMRTVANATEAFTVRGGGLGAFPSARQARVVWYGLDD
                     PRGRLRLLAQRMHAALGIEGSPRFRGHLTLARSRERFGGPNLAEWFSSADAPIADFRV
                     DRLVLCRSHLGQGPARYEPLAEAHLVPAAAHV"
     gene            4606..5109
                     /locus_tag="VFI12_04500"
     CDS             4606..5109
                     /locus_tag="VFI12_04500"
                     /inference="COORDINATES: protein motif:HMM:NF037785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00725 family protein"
                     /protein_id="HET7055699.1"
                     /translation="MSELTTDRPVYVAVIGDGDPHGAEGQRILSWAEEVGERLARAGA
                     VVVTGGLGGVMEAASRGATNAGGLTIGILPGDDRSDANAFVRVPIATGLGVARNLVVV
                     TASDVVIAVGGRYGTLSEIGLALRQGRGVVTLGSWRVGLEQGLTNPNVHIAGDPSSAV
                     ERALRPA"
     gene            complement(5140..6714)
                     /locus_tag="VFI12_04505"
     CDS             complement(5140..6714)
                     /locus_tag="VFI12_04505"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HET7055700.1"
                     /translation="MQRGQILIVTAAALLVLISVAALVVDLGFSWMLHRQEQNAADPA
                     ALAAARFIGDQDPVTGDQTFDFGLAGQAACFYAIQNKLVDPSNTNCDPALDPDDATVT
                     VHYPPDATSPDFVGDTGHVQVIITKNRDTFFSRVFGIGRMTITAQAVAARVRGAANTH
                     SLIALKPDGCSTAEVQGTALVHIYPKPGYTGPGGFVQVNANCGTPTSDDACGTSGGSG
                     SLDVNGTADLYATKVNVHGSCKGSVDEPHGLLDEAASQVADPLGGLVFPSILGQPGAR
                     CGGPSAPQTASTGTASKGCGGAPSVDWQSSPCPPPSTGDCVTLDPGVYYGGWTIDNDL
                     SVTLKPGIYVIAGGGIRFIGTSSLTSLAGTGPPAPVLIYNTDNPSQCPPSTSQWHCQD
                     DLSLKADRLQLAGLKADAPCPPISTTGGCPFGGMVIWDDPKGAQGLTGSGLIHVEGNS
                     ELFISGTIYAPRSAVEILGTSSENTTTQVCPTTATQIAAVQIISWTWKIGGTGDVCMP
                     YDPADLYKINRRGLVE"
BASE COUNT         1225 a         2229 c         2264 g         1175 t
ORIGIN      
        1 tcctcgcgcg cgccgtcgcc ggtgaggcag gcgtgccgtt cttctcgatc tcgggctccg
       61 agttcgtcga gatgttcgtg ggcgtcggcg ccagccgcgt gcgcgacctg ttcgaccagg
      121 cgaagcggaa cagcccgtgc atcgtgttcg tcgacgagat cgacgcggtg ggccgccagc
      181 gcggtgccgg cctcggcggc tcgcacgacg agcgcgagca gacgctcaac cagatcctcg
      241 tggagatgga cggcttcgac acgaacacca acgtgatcgt cgtcgccgcc accaaccggc
      301 cagacgtcct cgacccggcg ctgctgcgcc cgggccggtt cgaccggcag gtcatcctgg
      361 accgcccgga catgaagggg cgcaccgcga tcctcaaggt ccacaccaag ggcaagccgc
      421 tcgacaagac cgtgtcggtc gaggaggtcg cgcggcagtc gcccggcttc tccggcgcgg
      481 acctcgccaa cctcgtcaac gaggcggcca tcctcgcggc acgccgcaac aagaagtcgg
      541 tcggcatgtc cgagttcaac gaggcgatcg atcgcgtgat cgcaggcccg gaacgtcgga
      601 gccgcctgat caccgaggag gagaaggaga tcatcgccta ccacgaaggt ggtcacgcgg
      661 tggtgcagcg ggtgctcccc aagtgtgacc cggtgaccaa ggtcacgatc gtgtcgcgcg
      721 gcatggcgct gggctacacg atgagcctgc cgcaggagga ccgatacctc cactcgaaga
      781 gcgagttcga ggacaagatc gccggcatgc tcggcgggaa cgtctcggag cagatcgtct
      841 tcggtgacac gaccactggt tcgtcgaacg acatcgagaa ggccaccaac ctggcgcgcg
      901 cgatggtcac ccagtacggc atgagcgaga agctcggccc gctgaccttc ggcaagaagg
      961 aggagatgat cttcctgggc cgcgagatca gcgagcagcg caactattcg gacgagatcg
     1021 ccgccaagat cgacggcgag gtgcgggaga tcatcgatcg tgcctacgag cgtgccaagg
     1081 aggcgctgac acggcatcgg gccgtgctcg acaagctggc cgagctcctg atcgagaagg
     1141 agaccatcga ggcggacgag ttcgagtcgc tgttcgaggg gatcctcccg ccgcgcggca
     1201 cggcgaagcc actgctcccc gagggagagg ctgagcccga ggccgagccg acgggaaccg
     1261 acgaggcccc cgcgcgccgc cgcggcccgg ctccgcaacc cgcctgatcc gacccgtcgc
     1321 cacgtacgag gcccggccga aaggtcgggt ctcgtcattt ccggtcactt cctgtcttct
     1381 ccggtcattc ccgcatcggc ccatgcatcg ggcgcataca gctccgcatg aatggggggt
     1441 acgcggcgct tgatgacgcc ggcgtcatgc cgcccggcac gacggcggct tcgcgcggat
     1501 cggcatgcgt gcggtgcatg gggccggcaa cacgcaaccg gcacggaccg cgggtaggcc
     1561 gatcgggcca ttccggcgtc aggaatcgag gtcgtatact ccccgcgcgt tgcgtgcgcg
     1621 cacatcccat cgttgccctg aggagctgac atacagatga tcgacgagcc gatcgccaag
     1681 tccacgccag ccgccaccaa ctggcagctc gaggtggggg agggaaccct cgccccgcgc
     1741 tcggcgctcg acgtgctgac caacctcaac gagcggatcg catcgggcaa catcgacgag
     1801 ttccagccga tcgccaccgg cttcgtgccg ctcgacaagg tgatcggcgg cgggctgcga
     1861 ccgggcgagc tgatgctcat cggtggtgcg cagggccagg gcaagacgac catggcgctg
     1921 cagatggcgc gcaacgcggc cgcatcgggc caggcaacgg cgctgtacgt gtgcttcgag
     1981 cacgaggagg agtacctcat ccagcgcatg atcgcgatgg agtcggctct ctctcacctg
     2041 cccaagaaga gcggcgcggt caagatccag gacgtcaaga aggaggtcct gggcagctac
     2101 accgcatcgg ccgaagcggg aacccgcgcg aagttgggca ccaacccgcg attgcggccc
     2161 agcctggagc gcatcgcccg atacggcagc agtctcttcc tcctgcgcgg tacggccacg
     2221 cacagcacga tcgagaatct ccgtgcgctc gtgcaggagc atcgcggcga cgacgatcgg
     2281 cgcctgatcc tgttcgtcga ttacctgcag aaggttccgg tcttccccga gccggccgat
     2341 gaggccgaga aggtcacccg catcgtcagc ggtctcaagg acatcgccct cgcctgcgag
     2401 gtgccggtgg tcgcgatcgt ggctgccgac cgcgagggcc tcaaggccaa gcggctgcgc
     2461 aaccatcacc tgcgcggcag cagcgccctc aattacgaga gcgacatcat cctgatcctg
     2521 aacgacaagt acaacatcgt cgccaaggtc aacatcgagt tcaaccccta ccaggcgcag
     2581 cgcttccgcg actgggtggt ggcgacaatc gagaagaacc gcggcggcca ggacaacatc
     2641 gatctcgagt acgagaagct gttcgagtac agctgcttcg atcccaccgg gcgccagacc
     2701 tcggagaagc tgatcgagga gcggctctac aacgactgag tgccgcagct acgcggccgt
     2761 cgacgtcatg ccgcccagga tctggtagat ctccgtccga tacccgatcc ctgaggcggt
     2821 ccggctgagc cagcgcaggt agtccgggtc cttgcgtgcc aggtcgccca ggctccagcc
     2881 ggcgtatcgg ccgaaggaga gtcgcgagct cttctccttg gggatcaccc gttcggcgag
     2941 agaacgaggc cgcgcagccg gggcacgcag ctgcgcgtag gcctcgttga tctcacgcat
     3001 ccggcgggcg gcatacgaag aaccgtcgcg atccggatgg tatttcgatg ccaacgcccg
     3061 atacgcggcc tggaccacct ccggctccgc cgaagaaagg atctgcagga ccttgtgcgg
     3121 gtcggtcgtc aacacacgcg caggatgcgt ccgagcctgc ccggattcca tcgccttttg
     3181 gtccggtggc gccgcgcgcc tgcatcggtc gaccagacct ttccgcccag gtgcggtccg
     3241 atgcagtacc cgaatgggtc attcggcgcg gcaaccgctc tgcgctaggc ttagccgatg
     3301 tccagccttc aggccgtctt cctcgacatc ggcgataccg tcatgcgacc gaacccgtcg
     3361 tgggagcacg tctacgcggc ggccttcgcg gagttcgggg tcgaggtcac gcccgagcag
     3421 ctcctcccgg cactgcgcaa ggcctatcac cacggcggct ggggtttcgc tggcggcttc
     3481 gagccaacga tcgagacgag cttccggcgg acggtcgaga ttgaccaggc agccatcgtc
     3541 gacctcggtc ttggaccgat gccggacgcc ttcttccaac ggctctccga gctcttcacc
     3601 ctcaccagca actggcatgt ctttcccgat gcgccgggtg tcctcgacgg gctgcacgtt
     3661 cgcgggctga ccgtcggcgc ggtgagcaac tgggtctgga gcctgccgga gctgctccac
     3721 tcgctggagc tgatgagcaa gttcgacttc atcgcggtga gctcgcgaat cgggttcgag
     3781 aaaccgcacc cggcgatctt tcgctgggcg ctggagcagg cagacgccgc cgcggatgcc
     3841 gtgatccatg tcggcgacca cctcgacgcc gatgtccgag gtgcggaggc ggtcggaatc
     3901 agtggcgtgc tgatcgatcg cgtgggacgt tatgcgccag acgacctgcc ccaggacgtg
     3961 acggtcatcc attcgctcga tgagctgctg cccatcgtcg acgaacgcct cgccgaggct
     4021 gaccgcctgg cggggtgagc gagacgtggc gctgcttcat cgccgtgccg atcgcccagt
     4081 cgctgcggaa agacctggcc acgatggtcg cagctctcgg tgacgatccc gtaatggcgg
     4141 acttccgctg gaccgatccg gccgggtggc acctcaccgt cgcattcctc ggcgcaactc
     4201 cgccttccga cgtcgatcgg atcgccggcc tgatgcggac ggtcgcgaat gccacagagg
     4261 cattcaccgt ccgcggtggt gggctgggtg catttccttc agcacgccag gcgcgggtcg
     4321 tctggtacgg actcgacgac ccccgcggcc gcctgcgtct gctcgcgcaa cgaatgcacg
     4381 ccgcgctggg aatcgagggt tcgccacgtt ttcgcggaca cctcacgctc gcccggtctc
     4441 gcgagcggtt cggcgggccg aatctcgccg agtggttttc gtcagcagat gccccgatcg
     4501 ccgacttccg agtcgaccgt ctcgtcctgt gccggagcca cctcggccag gggccggccc
     4561 gttacgagcc tctcgccgag gcgcacctcg tgcccgcagc cgctcatgtc tgaactcacg
     4621 accgaccggc cggtctacgt tgcagtgatc ggggacggag atccgcacgg cgcggaaggg
     4681 cagcggatcc tttcgtgggc cgaggaagtc ggtgagcgcc tcgcacgtgc cggagcagtg
     4741 gtggtcaccg gcggacttgg cggggtgatg gaggccgcca gccgtggagc gacaaacgcc
     4801 ggcggtctga ccatcggcat tctgccgggt gacgatcgtt cagacgcgaa cgcattcgtc
     4861 cgcgtcccga tcgccacagg ccttggcgtt gcgcggaatc tcgtcgtggt cacagcgtcg
     4921 gacgtcgtca tcgccgtcgg agggcggtac gggacgctgt ccgaaatcgg gctagctctc
     4981 cgtcagggcc ggggggtagt gactctcggt tcgtggcgtg ttggcctgga acagggcctc
     5041 accaacccga acgttcacat agcaggcgat ccatcttctg ccgtggaacg ggcgctccga
     5101 cccgcttgat gagccggcga gtcggagcct cggttgattt tattcaacga gcccgcgtcg
     5161 attgatcttg tagaggtcag cagggtcgta gggcatgcag acgtcgcccg ttccgccgat
     5221 cttccaagtc caagagatga tctgcactgc ggctatttgg gtagcagttg tcggacacac
     5281 ttgggtagtg gtgttctcgc tcgacgtgcc gaggatttcc acggcagatc ttggcgcata
     5341 gatcgttccg gagatgaata gctccgaatt gccctcgaca tgaatgagtc ccgatccagt
     5401 gagaccctgt gcgccctttg ggtcatccca gatcaccatg ccgccaaatg ggcatccacc
     5461 cgttgtcgaa atgggaggac agggagcatc cgccttcagc ccggcaagct gaaggcgatc
     5521 agccttcaga ctcaggtcgt cctggcaatg ccactgtgat gtgctcggcg gacattgcga
     5581 tgggttgtcg gtgttgtaga tgaggacagg agccggtggc cctgttccgg caagcgacgt
     5641 gagcgaagag gtgccgatga atcggattcc gccacccgca atcacgtaga tgccgggctt
     5701 tagcgttacc gaaaggtcgt tgtcgatcgt ccatccaccg taatagacgc ccggatccaa
     5761 ggtcacgcag tcgccagttg agggcggggg gcaaggcgag gattgccagt ccacggacgg
     5821 ggcgccgcca caccccttcg atgcggtccc ggttgaggcc gtctgcggtg cgctgggtcc
     5881 accgcatcgt gcgcccggtt ggcccaggat agacggaaat acgagcccac caagcgggtc
     5941 ggcaacctgt gacgccgcct cgtcgaggag accgtgaggc tcatccacgc tgcccttgca
     6001 actgccgtgg acattgacct tcgtggcgta gagatccgcg gttccgttga cgtcaagcga
     6061 tccgctgccg ccgctcgttc cgcaagcgtc atccgaggtc ggtgttccac agttcgcatt
     6121 cacctgaacg aatcccccgg gacccgtgta cccgggcttg ggatagatat ggacgagcgc
     6181 cgtgccttgt acctcagcgg tgctgcagcc gtctggcttg agggcaatca gagagtgcgt
     6241 atttgccgct ccccgcacgc gcgccgcgac agcctgcgcg gtgatcgtca tacgaccgat
     6301 cccgaaaaca cgcgaaaaga aggtgtcgcg attcttggtg atgatgactt gaacgtggcc
     6361 ggtatcgccc acaaagtcgg gagatgtggc gtcgggcggg tagtgcacgg taacggtcgc
     6421 atcgtccgga tcaagcgccg gatcacagtt cgtgttcgat ggatcaacga gcttgttctg
     6481 tatcgcgtag aagcacgcag cctgaccagc caacccgaaa tcgaaggttt gatcgcccgt
     6541 aactggatcc tggtccccaa tgaaacgagc cgcagcaagt gcggctggat ccgccgcgtt
     6601 ttgctcttgc ctatgcagca tccacgagaa gccgagatcg acgaccaagg ctgccacact
     6661 tatcagaacg aggagcgcgg ccgccgttac gatcagaatt tggccgcgct gcatgcttcg
     6721 tcgacgatgc ttcattgctg gtactggagt gcctccgtaa tgactgccct cagcgtgagc
     6781 gtcttgggaa tgagcaagaa cccggcgagc ggcggtcgcc agtcgtagca ggcgtaaacc
     6841 gtgacctgat tcgcattgcc actggctgca gtgttgtaac tgctggccat gtc
//
LOCUS       DASSGX010000258         3093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_375903, whole genome shotgun sequence.
ACCESSION   DASSGX010000258 DASSGX010000000
VERSION     DASSGX010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375903"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VFI12_04510"
     CDS             <1..253
                     /locus_tag="VFI12_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055701.1"
                     /translation="VISDLVYPTDSYVQLIARLGTREIVGARSPFVIFQGEPDAPTIT
                     PTESAVATNTSVPTTAPTDVPMATDTPTVTDTPVPTTAP"
     gene            474..794
                     /locus_tag="VFI12_04515"
     CDS             474..794
                     /locus_tag="VFI12_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055702.1"
                     /translation="MTHHEHGDQPTRIEQQNLDAQDTLMTARQEDQRHPWESALNGDE
                     LASMPYLAPGTELKVDQSYLNLNQLNGGPFTPSGRRTVQDGDRIVAQSDIDPPLWRRL
                     VGEG"
     gene            824..1309
                     /locus_tag="VFI12_04520"
     CDS             824..1309
                     /locus_tag="VFI12_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055703.1"
                     /translation="MSEDFSPGDARKPYSDIGGPEAKHAQTHDVRRETVTNPKGPEPV
                     DTSFAEQMEPGTPRDPGSYADNSTPGIDEKVLHDRLPQLTNDELARLGVLEPGTQLEQ
                     GSVYLDLNRLDQGPFKALGRTASANERLIAKQSTDYILWNRLAGEDVEPGIERPDREH
                     H"
     gene            1312..1782
                     /locus_tag="VFI12_04525"
     CDS             1312..1782
                     /locus_tag="VFI12_04525"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HET7055704.1"
                     /translation="MAEPLDPVSGRETAPASIEVVRRLNAPAEEVFRAWVDPALLTQW
                     LAEKAYVHPCVDGHFRLETDKPGTVPQPYVYSGDYRAFIPGRRIVATWVYNGPHPEDL
                     GESLITIDLTPIDDRATEMRFREEWTGLADPTGREASRQKWLAAFDRLERVISS"
     gene            complement(1914..>3093)
                     /locus_tag="VFI12_04530"
     CDS             complement(1914..>3093)
                     /locus_tag="VFI12_04530"
                     /inference="COORDINATES: protein motif:HMM:NF044379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HET7055705.1"
                     /translation="DAHRGRIAIGRVHRGVIRAGETLVHIAPDGTETRQRITDLATFE
                     GLGKRRVEEALAGDIVAVAGFADAKIGATLADPNAPEALPTIDIEEPTLKLTFGVNTS
                     PFAGKEATYSTSRQLRERLYRELETNLSLRVEPTEQADVFAVSGRGELHLSVLIETMR
                     REGYEFQVSRPEVITKEIDGVVMEPVEHLEIETVEDSVGVIMDLVGTRKARMLDMVND
                     GRGSVRLEFAIPTRGLIGLRNAFLTATKGHAVMGSRLIGYEPWLGTIPNNRTGALVAS
                     EPGQVLAHGVANAQERGITFTEPGEDVYMGQIVGQQPRQGDMVVNVCRAKKLTNMRAS
                     TADIAIKLVPAVKLSLEQSLDFLADDELLEVTPKAFRLRKRLLNENDRAKERKRLSQS
                     "
BASE COUNT          554 a         1023 c         1009 g          507 t
ORIGIN      
        1 cgtcatttcc gatctggtct acccgaccga cagttatgtg cagttgattg cacggctcgg
       61 cacacgggag atcgtgggag cgcgatctcc attcgtgatc ttccagggtg agccggatgc
      121 cccaaccatc acgccgacgg agtcagccgt ggcaacgaac acgagcgtgc cgaccacggc
      181 tccaaccgac gtacctatgg cgaccgatac gcctacggtg accgatacac cagttccaac
      241 gacggcgcct tagcaacgca agtccaggtc cggaggcggg atcggccctc tcgatctcgc
      301 cgccatcgtc aagacgcaaa tggagcggcc ggaatggcac cgctccattt gcgtgttctg
      361 gcccagacgc cgcggtccgg tctgtagtcg gcttcgcctg ctttggcacg gcgggttgct
      421 acggttatcg ttcgggtccg cgcctgccag ccgggccatc gaaaggacac cccatgacac
      481 accacgagca tggcgaccag ccaaccagaa tcgagcagca gaatctcgat gcgcaagaca
      541 ccctgatgac cgcgcggcag gaggaccagc gccacccgtg ggaatcggcg ctgaacggtg
      601 acgaactcgc gtcaatgccg tacctcgcgc cgggcaccga gctgaaggtg gaccagtcct
      661 acctcaacct gaaccagctc aacggcggac cgttcacgcc gagcggccgg cgcaccgtcc
      721 aggacggcga ccggatcgtc gcgcagagcg acatcgatcc cccgctctgg cgccggctcg
      781 tcggcgaggg atagcgcggg tcggcaacgg agggaggaat ggaatgtctg aagacttcag
      841 cccgggcgat gcccgcaagc catattccga tatcgggggt ccggaggcca agcacgccca
      901 gacccacgat gtccgccggg agacggtgac gaacccgaag ggtcccgaac cggtcgacac
      961 gagcttcgcc gagcaaatgg agccggggac gccgagggat ccgggaagct acgccgacaa
     1021 cagcactccg ggtatcgatg agaaggtcct gcacgatcgg ttgccgcagc tcacgaacga
     1081 tgaactggcc cggctcggcg tgctcgaacc cggtacgcag ctcgaacagg ggagcgtcta
     1141 cctcgacttg aacaggctcg atcaggggcc gttcaaggcg ctcggacgta ccgcctcggc
     1201 caatgagcgg ttgatcgcca agcagagcac ggattacatc ctctggaatc gcctcgccgg
     1261 cgaagatgtc gaacccggca tcgaacggcc ggaccgcgag catcactagc cgtggctgag
     1321 cccctcgatc ccgtatccgg gcgggagaca gcgccagcca gcatcgaggt agtgcggcgg
     1381 ctgaacgcac cggcggagga ggtgttccgg gcctgggttg atcccgcgct gttgacgcag
     1441 tggctcgcgg agaaggccta tgtgcatccg tgcgtggatg gccacttccg gctcgagacg
     1501 gacaagcccg ggacggtccc gcaaccgtac gtctactcag gtgactaccg cgcgttcatc
     1561 cccggtcgtc ggatcgtcgc cacctgggtc tacaacggcc cgcatcccga agacctcggc
     1621 gagtcgctga tcacaattga tctgacgccg atcgacgatc gcgcgaccga gatgcggttt
     1681 cgggaggaat ggaccggcct cgccgatccc accgggcggg aagcgtcccg ccagaaatgg
     1741 ctcgccgcct tcgaccgcct ggaacgcgtt atatcgtcgt agtggtgggg ctccgtacct
     1801 gccttgagct gccctggcgt ccggtgggta gtgcaaatcc cggtgctcag tgcgggtacg
     1861 gagctccgcc actacgtctc aagtggactg cctcacgact cacgactcac gactcacgac
     1921 tgggagaggc gcttgcgctc cttggcccgg tcgttctcgt tgaggaggcg cttgcggagg
     1981 cggaacgcct tcggggtgac ctcgagcagc tcgtcgtcgg ccaggaaatc gagactctgc
     2041 tcgaggctga gcttgaccgc cgggaccagc ttgatcgcga tgtcggccgt gctggcccgc
     2101 atgttggtca acttcttggc ccggcagacg ttgacgacca tgtcgccctg gcgcggctgc
     2161 tgcccgacga tctggcccat gtaaacatcc tcgcccggct cggtgaaggt gatcccgcgc
     2221 tcctgggcat tcgccacgcc atgcgcgaga acctgcccgg gctcgctggc gacgagcgcg
     2281 ccggtccggt tgttcgggat cgtgcccagc cacggctcgt agccgatcag gcgggagccc
     2341 atgaccgcgt ggcccttggt cgcggtcagg aaggcgttgc ggaggccgat cagaccgcgc
     2401 gtcggaatcg cgaactcgag ccggacgctc ccgcggccat cgttgaccat atccagcatc
     2461 cgtgccttgc gagtgccgac caggtccatg atcacgccga ccgaatcctc gaccgtttcg
     2521 atttcgaggt gctcgaccgg ctccatcacg acgccgtcga tctccttcgt gatgacctca
     2581 ggacgggaga cctggaactc gtacccctcg cgccgcatcg tctcgatcag gaccgaaagg
     2641 tgcaactcgc cacgaccgga gaccgcaaag acatcggcct gctcggtggg ctcgacacgg
     2701 agggagagat tcgtctccag ctcacgatag agccgttcgc gcagttggcg ggaggtcgag
     2761 taggtcgcct ccttgccggc gaagggcgag gtattgacgc cgaaggtcag cttgagcgtc
     2821 ggttcctcga tatcgatcgt cggcagggct tcgggtgcat tcgggtccgc cagggtcgcg
     2881 ccgatcttgg catcagcgaa gccggccacc gcgacgatat cgccggcgag ggcctcctcg
     2941 acccggcgct tgccgagtcc ctcgaaggtg gccaggtcgg tgatccgctg gcgcgtttcc
     3001 gtaccgtcgg gcgcgatgtg gaccagcgtt tcaccggccc ggatcacgcc gcgatgcacc
     3061 cgcccgatcg cgatccggcc acggtgcgcg tcg
//
LOCUS       DASSGX010000259         3413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_593466, whole genome shotgun sequence.
ACCESSION   DASSGX010000259 DASSGX010000000
VERSION     DASSGX010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593466"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /locus_tag="VFI12_04535"
     CDS             <1..932
                     /locus_tag="VFI12_04535"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HET7055706.1"
                     /translation="PILNASMAGTATGALAAAVSEAGGFGMIGGTNAGGAEWLREQIR
                     IARSLTSRPFGVGFISAFPDTEELARVALDEGVAAINHSFADPTPYVEATHAAGAKLF
                     VQVQTLALAVRAAEAGADVIIAQGADAGGHAGAIGTFALLPAVVDAVAPIPVVAAGGI
                     ADGRGLAAALLLGAQGAWMGTRFVTSHEWGGPSWEQEAVLAATSDDTVQTRLYDLIGE
                     RPFPAENPDRMLRNAFIDRWAGRESEIPTHRDELQAEIAAGNERADLAVAGISAGVGA
                     GLITSAQPAGDIVRTIIQEAETLLRKRPKALLG"
     gene            1018..1428
                     /gene="tnpA"
                     /locus_tag="VFI12_04540"
     CDS             1018..1428
                     /gene="tnpA"
                     /locus_tag="VFI12_04540"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HET7055707.1"
                     /translation="MSYWRLFYHLVWATKNREPLIDDDVQAGVERSFHLTSNDLGLIV
                     HATGFMPEHVHLSVSIPPTIAIAEVVRRLKGASSRGVNRSNSAPAFGWQDGYGVLSFG
                     EKNLPQVNAYILNQKEHHASGTVIAALERDTETR"
     gene            1690..>3413
                     /locus_tag="VFI12_04545"
     CDS             1690..>3413
                     /locus_tag="VFI12_04545"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HET7055708.1"
                     /translation="MEFQRYSRRQFNRSLAFGAVALGAAATPVATRAQGVIVVEPPGC
                     ETGACPGPIRIGDQLTIKSHRVDVQVTNQVATTKIVQLFFNQNDWVAEGTYIFPIPDG
                     ATVSKFTMTVNGQQIEAKVLTAEEAAAIYDQIVRQMRDPALLQYVGQGAIQASVFPIP
                     PGETREVTITYEQVLTAEMGLIRYVYPLNTEQYSAMPLEQVSVRVEVTSKDPVRAIYS
                     PSHSVAVTQDSETHFVAGWEAANVWPSDDFELFYTVATDQIGANLVSYVDPVSREGFF
                     MLLAAPGIGTGQAVVAKDVIIVLDTSGSMEGDKIVQAKQAVSYVLGHLNAEDRFGVIE
                     FSTGVRFYDQQLRGASEAAVAASWVETLPATGGTDINQALLKAMEMADRERPTYVIFL
                     TDGLPTEGEVDSTVILSNVEAASPGNVRLFSFGVGDDVDTFLLDSLVQQENGVSSYVR
                     PGERIDDEVSLFYAKVGSPVLTDLQLAFSGVQVQELYPNPLPDLYAGSQLTLVGTYAT
                     GGPTTVTLTGQVNNTTSAYVYEGQALTTVPGGPNAFLPRLWATRKIGYLLTQIRLHGE
                     NPELIQAVV"
BASE COUNT          578 a         1150 c         1128 g          557 t
ORIGIN      
        1 gcccgatcct caacgcctcg atggccggga ctgcgaccgg cgcgttggcc gccgcggtct
       61 ccgaggcggg cggcttcggc atgatcggcg gcacgaacgc cggcggcgcg gagtggctgc
      121 gggagcagat ccggatcgcc cggtcgctca cctcgcggcc cttcggggtg ggctttatct
      181 ccgcattccc ggacacggag gagttggccc gggtggcgct ggacgagggg gtggcggcga
      241 tcaaccactc gttcgccgac ccgacgccct acgtcgaggc gacccacgcc gcgggggcca
      301 agctcttcgt tcaggtgcag acgctggcgc tggcagtccg tgcggcagag gccggggccg
      361 atgtcatcat cgcccagggt gctgatgccg ggggccacgc cggcgcgatc gggaccttcg
      421 cgctgctgcc cgccgtcgtc gatgccgtcg cgccgatccc cgtggtcgcc gccggcggca
      481 tcgcggacgg ccgtggcctg gccgcggcgc tcctgctcgg cgcgcagggg gcgtggatgg
      541 ggacccggtt cgtgaccagc cacgaatggg gcgggccgtc ctgggagcag gaggcggtcc
      601 tcgccgcgac gtccgacgac accgtccaga cgcgcctcta cgacctgatc ggcgagcgcc
      661 ccttccccgc cgaaaacccg gaccgcatgc tgcgcaacgc cttcatcgac cgctgggccg
      721 gtcgcgagtc ggagattccc acccatcgcg atgagcttca ggccgagatc gcggcgggca
      781 acgagcgcgc cgatttggca gtggccggaa tcagcgcggg cgtcggcgcg gggctgatca
      841 cctcggcgca gccggccggc gacatcgtgc gcaccatcat ccaagaggcc gaaaccctcc
      901 tgcgcaagcg gccaaaagca ttgcttggct agagcagttc cgccgggggt tgaaaccccc
      961 gtctcagcaa cggaagtcgg ttgaaaccga ctgggcgtgc gatttgtggg agaagcgatg
     1021 tcgtattggc ggttgttcta tcacctggtc tgggccacga agaatcgcga accattgatc
     1081 gatgacgacg tgcaggcagg ggtggagcga tcatttcatt tgactagcaa tgatctcgga
     1141 ttgatcgttc acgccaccgg cttcatgccg gagcatgtgc acctgtccgt gtcgataccg
     1201 ccgacaatcg cgatcgctga ggtcgtgcgg cgattgaagg gcgcttcgtc gcgtggcgtc
     1261 aatcgatcga atagcgcacc tgccttcggc tggcaggacg ggtatggcgt tctctccttc
     1321 ggcgagaaga atctgcccca agtgaacgca tacatcctca atcagaagga gcatcatgcc
     1381 agcggcacgg tgattgctgc gttggaacgg gacaccgaga ctcgataacg gcccgttacc
     1441 agtcggtttc aaccgacttc cgactgtgag acgggggttt caacccccgg cacgaccagc
     1501 tatctggatt aaccaagcta catccataac ccccggcttc cggcggtgct ccgtcatctc
     1561 accacccaat ggaacttcct cgcccctccc ggcgtctctt tgccagggga acgggctcgg
     1621 ttgtgtggtt tgcggcgatc cagggttgga tcgccttccc ggtcaagaga ccgacgcagg
     1681 tggtgtgaga tggagttcca gcgatattcc cggcggcagt tcaaccggtc gctcgcgttc
     1741 ggcgcagtgg ccctcggcgc agcggcgacg cccgtcgcga cccgggccca gggcgtgatc
     1801 gtcgtcgagc cgcccggatg cgagaccggc gcctgtcccg gcccgatccg gatcggcgac
     1861 cagctcacga tcaagtcgca ccgggtcgat gttcaggtca ccaaccaggt cgcgactacg
     1921 aagatcgtcc agctcttctt caaccagaac gactgggtgg ccgaggggac ctacatcttc
     1981 ccgatcccgg acggcgcgac ggtctcgaag ttcacgatga cggtcaatgg ccagcagatc
     2041 gaagccaagg tcctgaccgc cgaagaagcc gccgcgatct acgaccagat cgtgcgccag
     2101 atgcgggacc ccgccctgct ccagtacgtc ggccagggcg cgatccaggc gtcggtcttc
     2161 ccgatcccgc ccggcgagac ccgcgaggtg acgatcacct acgagcaggt gctgacggcc
     2221 gagatgggcc tgatccgcta cgtctacccg ctcaacaccg agcagtactc ggcgatgccg
     2281 ctcgagcagg tcagcgtccg ggtcgaggtc acgtcgaagg acccggtgcg cgcgatctac
     2341 tcgccgtcgc actcggtcgc cgtcacgcag gacagcgaaa cgcacttcgt cgccggctgg
     2401 gaggccgcca acgtctggcc gagcgacgac ttcgagctgt tctacaccgt cgcgactgac
     2461 cagatcggcg cgaatcttgt cagttacgtc gatccggtaa gccgcgaggg cttcttcatg
     2521 ctcctcgccg cgcctggcat cgggaccggc caggcggtcg tcgccaagga cgtgatcatc
     2581 gtcctcgaca cctccggcag catggagggc gacaagatcg tccaggccaa gcaggcggtc
     2641 agctacgtgc tcggccacct caacgccgag gaccgcttcg gcgtgatcga attcagcacg
     2701 ggcgtccgct tctatgatca gcaattgcgc ggggcgagcg aggcggcggt tgcggcgagc
     2761 tgggtcgaga cgttgccggc caccggcggg accgatatca accaggccct gctcaaggcg
     2821 atggagatgg ccgaccgcga gcggccgacc tatgtcatct tcctgaccga cggcctgccg
     2881 accgagggcg aggtcgacag cacagtcatc ctctccaacg tcgaggcggc ctcgccgggc
     2941 aacgtccggc tgttcagctt cggggtgggc gatgacgtcg ataccttcct gctcgactca
     3001 ctcgtccagc aggagaacgg cgtcagcagc tacgtccggc ccggcgagcg gatcgacgac
     3061 gaggtttcgc tcttctacgc caaggtcggc agcccggtcc tgaccgatct ccagctcgca
     3121 ttcagcggcg tgcaggttca ggagctctac ccgaacccgc tgcccgatct ctacgccggc
     3181 tcgcagctca cgctggtcgg cacctacgcg accggcggac cgacgacggt gacgctcacc
     3241 ggacaggtca acaacacgac gtcggcctac gtctacgagg gccaggcgct gacgaccgtg
     3301 ccgggcgggc cgaacgcctt cctgccccgc ctctgggcga cgcgcaagat cggctacctg
     3361 ctgacccaga tccggctcca cggcgagaac ccggagctga tccaggcggt agt
//
LOCUS       DASSGX010000260         2593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1357080, whole genome shotgun sequence.
ACCESSION   DASSGX010000260 DASSGX010000000
VERSION     DASSGX010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1357080"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="VFI12_04550"
     CDS             <1..838
                     /locus_tag="VFI12_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055709.1"
                     /translation="QVPRLQEALARAGFASLLYWSPAMRDYRLDPADVENLALAYEWF
                     VEQETIDPERSGMIGTCVGGAFVLMAAAQERVRDRVRFVGAFAPFASMHTLAEDIATA
                     TRPVNGVREPWDVDPLTRTVYVHSLTAHLEPIEAARLREAMAEPTGRVDPGGLSPEAR
                     VVYPLLTALTLDEVRAALNVLPQELRDRLDAMSPLSYLPGLRAPLLVLMHDRDDPVIP
                     IGESKLLWAALAGRPGLRCTEFTMFKHLDPTKVKLRIPQLLRELNRFYRSLYPIFRQT
                     TV"
     gene            complement(867..1286)
                     /locus_tag="VFI12_04555"
     CDS             complement(867..1286)
                     /locus_tag="VFI12_04555"
                     /inference="COORDINATES: protein motif:HMM:NF024559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3995 domain-containing protein"
                     /protein_id="HET7055710.1"
                     /translation="MLIFAAMSAYWAAGGRIGVETIGGTVESYGDEGGATFVALLWLI
                     AALKVLAALVPLTLAERWTIVVPRRLLRLATLAMGIGMVLYAGANFGARVIMAVGLID
                     TPESMHSAAARWHLYVWNPWWLLGGILYVAVARLARA"
     gene            complement(1338..>2593)
                     /locus_tag="VFI12_04560"
     CDS             complement(1338..>2593)
                     /locus_tag="VFI12_04560"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HET7055711.1"
                     /translation="RHVAARSYVHTRGAQNYREVYDIIHPRDVLLEPRGLRRTPFYER
                     QKELGAVFTESNGWERPLWYEANADLPVPEGAERSGWSAKHWSRFAAAEHVATRTSAG
                     LFDMSTFTKLHVYGPGALAAMELVSCSNVNKPVGGVSYALLLNERGGIESDLTVVRAD
                     TNHFTLLCGSGSGPRDYAWVSRRMLDLPEVRVEDITSASCALGLWGPTAETILQPLVE
                     ENLSIEAFPRFSAKWTYVAGVPVMLVRISYVGENGFELHVPTEFGGHLWEALWAAGRE
                     HGMVACGLTAMDSLRLEKGYLALGTDIRSEHTPQETGLGFAVSKKRTNYIGYEALRNR
                     PVEQRLCAMTFDDPGVVVIGKEPILHDNQVVGYITSANYGYSIGKSIAYGWLPANCAE
                     PGTELAIEWFGKQYPVTVQKVPLLA"
BASE COUNT          438 a          911 c          824 g          420 t
ORIGIN      
        1 gcaggtgccg cgcctgcagg aagcgctggc ccgggccggg tttgcctcgc tgctctactg
       61 gtcaccggcg atgcgcgatt accggctcga tcccgccgac gtcgagaacc tggcgctggc
      121 ctacgagtgg ttcgtcgagc aggagacgat cgacccggag cgcagcggca tgatcggcac
      181 ctgcgtcggc ggcgcgttcg tactgatggc cgcggcccag gagcgagtcc gggaccgggt
      241 ccggttcgtc ggcgcattcg ccccctttgc ctcgatgcac acgctggccg aggacatcgc
      301 aacggcgacc cgcccggtga acggcgtccg ggagccgtgg gacgtcgatc ccctgacgcg
      361 cacggtctac gtccactccc tgacggctca cctcgaaccg atcgaggctg cccggttacg
      421 cgaggcgatg gcggagccga ccggtcgcgt cgacccgggc gggttatcgc ccgaggcgcg
      481 cgtcgtctac ccgctgctga ccgccctgac gcttgacgag gtcagggcag cactgaatgt
      541 cctgccgcaa gaactgcgcg accggctgga cgcgatgtcg ccgctgagct acctaccggg
      601 cctccgcgcg ccgctactgg tcctgatgca cgatcgcgac gaccctgtga tcccgatcgg
      661 cgaatcgaag ctgctctggg ctgccctcgc cggccggccc ggcctccgct gcaccgagtt
      721 cacgatgttc aagcacctgg acccgacgaa ggtcaagctg cgcatcccgc aactgctccg
      781 cgagctcaac cgcttctacc gcagcctcta ccccatattc cgccagacaa ccgtctgaag
      841 ggacggcgga ccgcgccggg atcaccctat gcccgtgcca atcgagcgac ggcgacgtag
      901 aggatgccgc cgagaagcca ccaggggttc caaacgtaga gatgccagcg cgcggccgca
      961 ctatgcatcg attcgggcgt gtcgatcaga ccgacggcca tgatcactcg ggcgccgaag
     1021 ttcgcgccgg catagaggac catcccgatc cccattgcca aggtcgcgag ccggagcaag
     1081 cgtcgcggaa ccacgatcgt ccatctctcc gccagggtca gcggcaccag cgctgccaga
     1141 accttcagtg cagcgatcag ccaaagaagg gcgacgaacg tcgcaccacc ctcatctcca
     1201 taactctcga ctgttccgcc gatcgtttcg acgccgattc tgccgcctgc cgcccagtag
     1261 gcactcatcg cggcaaagat aagcatccat cccgcggcgg cccaggcaag tccccgttcg
     1321 gaccacatcc agccagtcta tgcgagcagc gggaccttct ggaccgtcac cgggtattgc
     1381 ttgccgaacc attcgatggc tagctcggtg ccgggttctg cgcaattcgc ggggagccag
     1441 ccgtaggcga tgcttttgcc gatggagtag ccgtagttgg cgctggtgat gtagccgacg
     1501 acctgattgt cgtgcaggat cggctccttg ccgatcacca ctacgccggg atcgtcgaag
     1561 gtcatcgcgc aaagccgctg ttcgaccggg cggttccgca acgcctcgta gccaatgtag
     1621 ttggtccgct tcttgctgac cgcgaagcca aggccagtct cttgcggggt gtgctcgctg
     1681 cgaatgtccg tcccgagcgc gaggtagccc ttctcaagcc ggagcgagtc cattgcagtc
     1741 aggccgcagg cgaccatccc gtgctcccgc cccgcggccc agagcgcctc ccagaggtga
     1801 ccgccgaact cggttggcac gtgcagctcg aagccgttct cgccgacgta cgagatccgg
     1861 accagcatga ccgggacgcc ggccacgtag gtccacttcg cggagaagcg cgggaaggcc
     1921 tcgatcgaga ggttctcctc caccagcggc tgcaggatcg tctccgcggt cggaccccaa
     1981 agcccgagcg cgcacgaggc cgacgtgatg tcctcaactc gaacttccgg caggtccagc
     2041 atccgccgcg agacccaggc gtaatcgcgc gggccgctgc cggagccgca aagcagcgtg
     2101 aagtggttcg tatcggcgcg aaccacggtc agatcggatt cgatcccgcc ccgctcattc
     2161 agcagaagcg cgtagctgac cccgccgacc ggcttgttga cgttgctgca cgagaccagc
     2221 tccatcgcgg ccagcgcgcc gggaccgtag acgtgcaact tggtgaaggt actcatgtcg
     2281 aacaggccgg cgctcgtccg ggtggcgaca tgctcggccg cggcaaaccg cgaccagtgc
     2341 ttcgcgctcc agcctgatcg ctccgcgccc tctggcaccg gcagatccgc gttcgcctcg
     2401 taccagagcg gtcgctccca gccgttcgac tcggtgaaga cggcgcccag ctccttctgc
     2461 cgttcgtaga agggcgtccg gcgcagtccg cgcggctcga gcaggacatc gcgcgggtgg
     2521 atgatgtcgt agacctcgcg gtagttctgc gcgccccggg tgtgaacgta gctgcgcgcc
     2581 gccacatgcc ggc
//
LOCUS       DASSGX010000261         2787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1246519, whole genome shotgun sequence.
ACCESSION   DASSGX010000261 DASSGX010000000
VERSION     DASSGX010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246519"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VFI12_04565"
     CDS             <1..417
                     /locus_tag="VFI12_04565"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HET7055712.1"
                     /translation="DPENRVTLGTDGAIRVHWTPNNTVTHRKFIRVAKDVFRDAGYRV
                     LIDRVMGIETNSHQVGTNRFGTDPAMSVLDPYCKAHDLDNLYVMDASFFPSSTAVNPA
                     RTIGAQAVRVADHLMERLGGTAQHVVRSATDVPAGA"
     gene            502..1566
                     /locus_tag="VFI12_04570"
     CDS             502..1566
                     /locus_tag="VFI12_04570"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET7055713.1"
                     /translation="MANQTAIRPQRVLVTGGAGFLGINLIRYLLDRDVDVTSLDIAPF
                     DYADVRDRVRIVTGDIRDKAAVELALADCDMVVHCAAALPLYAPEDIYTTDVVGTRIV
                     MASALERGVRRVVHISSTAVYGIPDHHPLREEDKLDGVGPYGQAKIQAEMVALELRAQ
                     GLVTPIIRPKSFIGPERLGVFSLLYDWALDGRGFPMLGSGKNRYQLLDVEDLCDAIWL
                     CLTLPDDVVNDTFNVGAAEFGTMGADYQAVLDEAGFGKMVRPVPALPAVLVLRLLEKI
                     GVSPLYKWVYETASKDSFVSIDKVRQQLGWRPKYSNVDALLRNFAWYKEHQAEFANAS
                     GVSHRVPWKQGAIGFLKRLF"
     gene            1578..2513
                     /locus_tag="VFI12_04575"
     CDS             1578..2513
                     /locus_tag="VFI12_04575"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HET7055714.1"
                     /translation="MANLAPAKANRERSAQAIRVRGMTLPPLIRAMRPLQWSKNSLVF
                     AALLFDRRVFQFDRLWHSLAAALVFCAVSSAIYLVNDIRDVEQDRHHPRKRFRPIAAG
                     EVSVGQAARVATVLLLAGLVTAFAVRIEFAAVIIAYIALMIAYSYGLKRMVILDVFAI
                     AAGFVLRAAGGALAISVPISPWLYVCTALGALFIGFGKRRNEIVTLEAAAGQHRANLD
                     EYSLPMLDQIIAIVSAAMLISYSLYTFDAANVKDSRAMMLTIPFVAYGLFRYLYLIYR
                     RGEGGSPEVLLIKDPGLISCIVGWVATSLVILYLA"
     gene            2526..>2787
                     /locus_tag="VFI12_04580"
     CDS             2526..>2787
                     /locus_tag="VFI12_04580"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HET7055715.1"
                     /translation="MDDLTRPQPPRAADPVALAYAFHVFSRETLIRKLEGLSDAAQRR
                     VALPSGGTMLGLLKHCIHVERLWIAYAMGGLDVEFPWTETDEE"
BASE COUNT          464 a          883 c          924 g          516 t
ORIGIN      
        1 gaccccgaga accgggtcac gctcggcacg gacggcgcaa ttcgtgtcca ctggacgccg
       61 aacaacacgg tcacgcaccg gaagttcatc cgcgtggcga aagacgtctt tcgcgatgct
      121 ggttaccggg tgctgatcga ccgggtgatg ggcatcgaga cgaactcgca ccaggtcggc
      181 acgaaccggt ttggaacgga cccggcgatg tcggtcctcg acccgtattg caaggcgcat
      241 gatctcgaca acctctacgt gatggacgcg tcgttctttc cgtcgtcgac cgcggtcaac
      301 ccggcgcgga cgatcggcgc gcaagcggtg cgggttgccg atcacctcat ggaacgcctc
      361 ggcggaaccg cccagcacgt ggtcagatcg gcgaccgatg tcccggccgg cgcgtagacc
      421 atccgccagc gaacacgtat gctcaatggt ggccgggaaa aggctccggc agttgctgaa
      481 tcacagtcag gcgaacaacg agtggcaaat caaaccgcga tccggccgca gcgggtgctg
      541 gtcaccggcg gcgcgggctt tctgggaatc aacctgatcc ggtacttgct tgatcgcgac
      601 gtcgacgtca cctcgctcga cattgccccg ttcgactacg ccgatgtgcg cgaccgggtc
      661 cggatcgtga ccggcgatat tcgtgacaag gcggcggtcg agctggccct ggcggattgc
      721 gatatggtcg tccactgtgc ggcggccctc ccgctatacg cgccggagga catctacacg
      781 accgatgtcg tcggcacccg gatcgtcatg gcgagtgctc tggagcgggg cgtcaggcgt
      841 gtcgtccaca tctcctccac cgcggtctac ggtatcccgg atcatcaccc gctgcgcgaa
      901 gaggacaagc ttgacggcgt cggtccgtac ggacaggcca agatccaggc cgagatggtc
      961 gcgcttgagc tgcgggcgca ggggctggtg acgccgatca tccggccgaa atcgttcatc
     1021 gggccggaac ggctgggcgt cttctcgctg ctttacgatt gggcgctcga tgggcgcggc
     1081 tttccgatgc tcggcagtgg caagaaccgg taccagcttc tggatgtcga ggacctctgc
     1141 gacgcaatct ggctctgcct gacacttcct gacgacgtgg tcaacgacac cttcaacgtc
     1201 ggcgcggccg agttcgggac gatgggcgct gattatcagg ccgtgctcga tgaggctggg
     1261 ttcggcaaga tggtccgacc agtccccgcg ctgccggccg tgctggtgct ccggctgctt
     1321 gagaagattg gtgtctcgcc gctctacaag tgggtgtatg agacggcctc aaaggactcg
     1381 ttcgtctcga tcgacaaggt tcgccagcag cttggatggc gaccgaagta ctccaacgtc
     1441 gatgccctgc tgcgcaactt cgcgtggtac aaggaacatc aggctgaatt cgcgaacgcg
     1501 agcggggtct cgcaccgggt gccgtggaag cagggcgcga tcggtttctt gaagcggttg
     1561 ttctagcgga gcgagcagtg gcaaacctgg cgccggcaaa agcgaatcgg gagcgaagcg
     1621 cgcaggcgat ccgggtgcgc ggtatgacgc tgccgccgct gatccgggca atgcggccgc
     1681 tccagtggtc caagaacagc ctcgtcttcg cggcgctgct gttcgatcgc cgcgtcttcc
     1741 agttcgaccg gctctggcat tccctcgcgg ccgcgctggt cttctgcgcg gtcagcagcg
     1801 cgatctacct ggtcaatgac atccgggacg ttgagcagga ccggcaccat ccccgcaagc
     1861 gattccggcc gatcgcggcg ggcgaggtca gcgtcgggca agccgcgcgg gtcgcgaccg
     1921 tgctgcttct cgccggtctg gtgacagcat tcgccgtccg gatcgagttc gcggcggtga
     1981 tcatcgccta tatcgccctg atgatcgcct attcgtatgg cctgaagcgg atggtcatcc
     2041 tcgatgtctt cgcgatcgcg gccggcttcg tgctgcgcgc cgccggcgga gccctggcaa
     2101 tcagtgtgcc gatctcgccc tggctctacg tctgcacggc tctcggcgcg ctcttcattg
     2161 gcttcggcaa gcggcgcaac gagatcgtga cgctggaggc ggcagccggg cagcaccgcg
     2221 cgaacctcga cgagtacagc ctgccgatgc tggaccagat catcgcgatc gtctcggccg
     2281 cgatgctgat ctcgtactcg ctctacacct tcgacgccgc gaacgtgaag gacagccggg
     2341 ccatgatgct gacgatcccg ttcgtcgcct acggcctgtt ccggtacctg tacctgatct
     2401 atcgccgggg cgaaggcggc agcccggagg tcttgctgat caaggacccg ggcctgatct
     2461 cgtgcatcgt cggctgggtg gcgacctcgc tggtgatcct ctatctcgcg tgaggactcg
     2521 aacgagtgga tgatctgacc cggccgcaac cgccacgcgc ggcggacccg gtggccttgg
     2581 cctacgcctt ccatgtcttc tcccgggaga cgctgattcg caagctggag gggctgagcg
     2641 atgccgcgca acggcgcgtg gcgctgccct ccggcgggac gatgctcggc ttgctcaagc
     2701 actgcatcca tgtcgagcgg ctctggatcg cctacgcgat gggcggcctc gacgtcgaat
     2761 tcccatggac cgaaacggat gaggaag
//
LOCUS       DASSGX010000262         2212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_376833, whole genome shotgun sequence.
ACCESSION   DASSGX010000262 DASSGX010000000
VERSION     DASSGX010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376833"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="VFI12_04585"
     CDS             <1..452
                     /locus_tag="VFI12_04585"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HET7055716.1"
                     /translation="ASKLKIPEDRLGSIEVHIHVPQGAIPKDGPSAGTTMATALVSAM
                     SGRPVRRDVAMTGEITLRGRVLPIGGVKEKVLGAHRAGISTIILPKDNEADMEDIPED
                     VRKQLTFHCVSTLDEVFAIALVPIGDQVETSPTLMEEMEDKAETMVA"
     gene            complement(470..>2212)
                     /locus_tag="VFI12_04590"
     CDS             complement(470..>2212)
                     /locus_tag="VFI12_04590"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HET7055717.1"
                     /translation="MHPDIIDRSQVSGTAGISRKFHEQAAAREMEKHYTKEKILEAYL
                     NQINLGRGWYGIDAGSRHYFGHPAAQLTLAEAATLAGLPKSQPTYDPISHPARARERR
                     NLILQKMAEQGYITQELADKTKLEPVNTAPNGGSSAPSGYFIDAVRQQAERAGIPVMN
                     GGYRLYTTLDPELQRDAVTAVVDGTNKIESQKGYKHLTQAAAKGNQSDYLQALAVAID
                     PQTGDVRALVGGRNYARAPFNRAVLARRQPGSSIKPIVYAKAIEDSIPANAVIPDTAL
                     TIDLPTGDQYKPTEIDGRFWGIHTWREGVPTATAMTMREGLIHSRNMVAIQLGLRAGM
                     DSVAALAQRLGITPPMDPVPASAIGASVVHPIDLVAAYTAFANNGAVVQPRFVTRIDD
                     LAGKTVFSRPPSTPQQILDPRVAFIMRDMMRDVAERGSGTQARKAVPEGIPVAGKTGT
                     TNDNVDVWFLGMTPDLVAGIWLGFDKPKTIASGAVGGGLAAPIWGQMMGRYYANRPTA
                     GWGPPPDGLVYAELDRTTANLATPMTPADQRYIEYFLPGTEPLELRNNPWNVPQWGPL
                     FMPTSRTPGGIPKH"
BASE COUNT          377 a          658 c          726 g          451 t
ORIGIN      
        1 atgcctccaa gctgaagatt cccgaggatc gcctcggttc gatcgaggtt cacattcacg
       61 ttcctcaggg cgccattcct aaggatggtc cgtccgcggg aacgacgatg gcgacggcgc
      121 tcgtgagcgc gatgtcgggc cggcctgtgc gaagggatgt cgcgatgacc ggggagatca
      181 cgctccgtgg tcgcgtgctg ccgatcggtg gcgtgaagga gaaggtcctc ggcgctcacc
      241 gtgccggaat ctcgaccatc attctgccga aggacaacga agcggacatg gaggacatcc
      301 cggaagacgt ccgcaagcag ctcaccttcc attgcgtgag cacgctcgat gaggtcttcg
      361 ccatcgcgct ggtcccgatt ggcgatcagg tcgagacttc gccgacgctg atggaagaga
      421 tggaagacaa ggccgagacg atggtggcgt aacccaccat cgccgggatt tagtgttttg
      481 gaatgccgcc tggggttcgc gacgtcggca tgaagagcgg accccactgc ggcacgttcc
      541 acgggttgtt acgaagctct aaaggctccg tgcccggcag gaagtactcg atgtagcgct
      601 gatcggccgg tgtcatcggc gtggcgaggt ttgccgtggt acgatcgagc tccgcgtaga
      661 ccagtccgtc cggcggtggt ccccacccgg ccgtcgggcg attggcgtaa tagcggccca
      721 tcatctgtcc ccagattggc gcggcgagcc cgccgcccac tgcgccggac gcaatcgtct
      781 tcggcttgtc gaagccgagc caaatgccgg caacgagatc gggcgtcatt cccaggaacc
      841 acacgtcgac gttgtcgttc gtcgtgcctg tcttgccggc gaccggaatg ccctcgggta
      901 ctgctttgcg agcctgcgtc ccactgcccc gctcagcgac gtcgcgcatc atgtcgcgca
      961 tgatgaaggc gacgcgcgga tcgagaatct gttgtggtgt cgagggcgga cgtgaaaaaa
     1021 cggttttccc cgcgaggtcg tcgatgcgag tcacgaaccg cggttgaacg acagcgccat
     1081 tgttggcgaa cgcggtgtac gcggcgacga gatcgatcgg atgtacgacc gaagcaccga
     1141 ttgcactggc tggaaccgga tccattggcg gcgtgatccc gagccgctgt gcgagcgccg
     1201 cgactgaatc catcccagcc cgaagaccga gttgaatcgc gaccatgttg cgcgagtgga
     1261 tgagaccttc gcgcatcgtc atcgcggtcg ccgtcggcac gccttccctc caggtgtgga
     1321 tcccccaaaa cctcccatcg atttcggtcg gcttgtactg gtctccggtc ggcaagtcga
     1381 tggtgagagc agtgtcagga atgacggcgt tcgcggggat gctgtcttcg atggcttttg
     1441 cgtagacgat cggcttgatc gacgaaccag gctggcgtcg cgcgaggacc gcacgattga
     1501 atggcgcccg agcatagttg cgaccgccga cgagagcgcg gacgtcgccc gtctgcggat
     1561 caatggcgac ggcgagtgct tgcaggtagt cgctctgatt ccctttcgcg gcggcctgcg
     1621 tgaggtgttt atagcctttt tgagactcga tcttgttggt cccgtcgacg accgccgtca
     1681 ctgcgtcgcg ctgcagttcc ggatcgagcg tcgtgtacag gcgatagccg ccgttcatca
     1741 ccggaattcc ggcgcgctct gcctgctgcc gcaccgcatc tatgaagtag ccggacggtg
     1801 ccgagctgcc gccgttggga gcggtattca ccggctcgag cttcgtcttg tccgcgagct
     1861 cctgcgtgat gtagccctgt tcggccatct tctgcagaat caggttgcgt ctctcacgag
     1921 cacgggccgg gtgtgagatg ggatcgtagg tcggctgcga tttcggaaga cccgcgagcg
     1981 ttgctgcctc ggcgagcgtc agctgcgccg cgggatggcc gaagtagtgc cgcgagccgg
     2041 catcgatacc ataccaaccg cgcccgaggt tgatctggtt gagatatgcc tcgaggattt
     2101 tttctttggt gtagtgcttt tccatctcgc gagccgccgc ctgctcgtgg aattttctcg
     2161 agatcccggc ggttccactg acctgagagc ggtcaatgat gtccgggtgc at
//
LOCUS       DASSGX010000263         4292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1030214, whole genome shotgun sequence.
ACCESSION   DASSGX010000263 DASSGX010000000
VERSION     DASSGX010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030214"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..881)
                     /locus_tag="VFI12_04595"
     CDS             complement(33..881)
                     /locus_tag="VFI12_04595"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HET7055718.1"
                     /translation="MRRVRWILAALLVPALMLSWSAIAFAQDYGDATPSPGASEGNQP
                     SVFVRQDPVLGAILTDAKGMTLYMWANDTTKGETTCYDKCAQNWPPYSASEPFSLPFT
                     VKGEITSVQRTDGASQIAFNGIPLYTFVKDTEPGQTNGQGVGDVWFVVHPDAQFGQTA
                     AAEATPTTAMTAGTPAAAGTVDVTLIDGVISASATTFTVGQKYTFNVANNGTIQHEFY
                     IEKAGAVDQPLKTDSGEEAEIKRFDPGQTMTLDFTFTEPGIYQFACHVPGHYPAGMAL
                     TIMVVQ"
     gene            1046..1648
                     /locus_tag="VFI12_04600"
     CDS             1046..1648
                     /locus_tag="VFI12_04600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7055719.1"
                     /translation="MALTWDLTTETVPDWMADDDDAAWAMRARTDRAAFGVLYDRYVD
                     RIYAYCRRRLQTQEAAEDATSQTFLKALGAIARYQPEAGTFRAWIFTIANRVVIDSYR
                     REKPAASLDGIGDVRDPESGPEHVALDREASRELYALVDRLPEEQANLIQLRLAGLTD
                     REIAGVLGRSYGAIRVAQHRAIKRLQELATNASATKRTRQ"
     gene            1645..2820
                     /locus_tag="VFI12_04605"
     CDS             1645..2820
                     /locus_tag="VFI12_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055720.1"
                     /translation="MTRPDQHRASPHDPDADRLSATWNDLVAGRQPVESDELTDAVQS
                     LHAAAPTYRPTSTFRDELREQLMHTAMPIEMPGVRQQPRLREQTAEPRLRVPTVLGGH
                     AVRWTAIAATIALLLTTLAAGYLGGLLPAGNGSGPTRNVAAPYLVNGTPVAGTPVSGT
                     PVANVCDTVGRYLPCGPNPEIGPGMFDPSRLPAEALDVTKAQLQGWEVEGSGTVTFSA
                     PHNPVTGVAVDVVVMGAYRAKFSAPVTVYRAYPGSGGVWLYPEAGAQIELSRGDSVSY
                     QIGTKTELENPFNAWTLKFKTALFYDGDPSPGNFGAAGNVQVRIDGDGTLPKPLAELG
                     APEISVEISYATLPEVGAPISGTTFLTPVIGPVASTQVLGGPNEGFVVWVSTNAPRG"
     gene            complement(2980..3387)
                     /locus_tag="VFI12_04610"
     CDS             complement(2980..3387)
                     /locus_tag="VFI12_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055721.1"
                     /translation="MERGNPPGTSDTFANTGDAGPIEKVHKGMHVVDAAGDDIGRVEF
                     VKMGDPQAATIGADADTRDGLAENFAEIFGWDGEPDLPPALAARLMRVGFIKIDSKGL
                     FAKDRYVAADRIAGVSGDTVRLTVNKDDMAEES"
     gene            complement(3913..>4292)
                     /locus_tag="VFI12_04615"
     CDS             complement(3913..>4292)
                     /locus_tag="VFI12_04615"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HET7055722.1"
                     /translation="LAPDGDHLDVLILVEEPTFPGCMIEIRAIGGLDMSDEKGSDFKV
                     LAVPVGDPRFAHVNSLEDLGQHWLREIETFFDTYKLLEPKQTNVQGWHDIDETRQVIA
                     DCRAAYERSRAGEESSSRAGGTL"
BASE COUNT          734 a         1393 c         1438 g          727 t
ORIGIN      
        1 atggccgccg gcgcgcgaac cgagtgcgac ggttactgga caaccatgat cgtcagcgcc
       61 atgccggccg gatagtggcc gggcacgtgg caggcgaact ggtagatgcc gggctcggtg
      121 aaggtgaagt cgagcgtcat cgtctgcccg ggatcgaagc gcttgatttc cgcttcctcg
      181 ccggaatcgg tcttgagcgg ctggtccacc gcgccggcct tctcaatgta gaactcgtgc
      241 tggatcgtgc cgttgttggc gacattgaag gtgtacttct ggccgaccgt gaaggtcgtg
      301 gccgaggccg agatcacgcc gtcgatcagc gtgacgtcaa ccgtgccggc ggcggccggc
      361 gtgccggcgg tcattgccgt agtcggggtc gcctctgcgg cggccgtctg cccgaactgc
      421 gcatccgggt gcacgacgaa ccagacatcg ccgacaccct gcccgttggt ctggcccggc
      481 tcggtatcct tgacgaaggt gtagagcggg atgccgttaa aggcgatctg gcttgcgccg
      541 tccgtccgct ggaccgaggt gatctcgccc ttgaccgtga acggcagcga gaacggctca
      601 ctcgcggagt atggcggcca gttctgggcg cacttgtcgt agcaggtggt ctcgcccttg
      661 gtcgtgtcgt tggcccacat gtagagggtc atgcccttgg catcggtcag gatcgcgccg
      721 agcaccgggt cctgccggac gaagacggag ggctgattgc cttcactcgc gcccgggctc
      781 ggggtcgcat cgccgtaatc ctgggcaaac gcgatcgcgc tccagctgag catgagcgcc
      841 ggcaccagca gtgccgccaa tatccatcgc acacgtcgca tggtctctcc tccatggcga
      901 aaactcggcc acgagccgga cgccggtgac cggatgaggt tcttacggag cagaagcggc
      961 tttggatgag gcagcggtaa cagctccgcg acaatcgcgt atagcttcct gatcgcacgg
     1021 tgcgatcggg ggtggtgatg ggttggtggc gttgacctgg gacctgacaa ccgaaacggt
     1081 cccggactgg atggcggatg acgacgatgc ggcctgggca atgcgcgccc gaaccgatcg
     1141 ggcggcgttc ggcgtcctct acgaccgcta tgtggatcgc atctacgcct actgccgccg
     1201 gcggctgcag acgcaggagg cagcggagga cgcgaccagc cagaccttcc tcaaggcgct
     1261 cggcgcgatc gcccggtacc agccggaggc gggcacgttc cgggcctgga tcttcaccat
     1321 cgccaaccgc gtggtgatcg acagctaccg gcgcgagaag ccggcggcct cgctcgacgg
     1381 catcggcgac gttcgcgacc ccgaatcggg gccggagcac gtcgcgctcg accgggaagc
     1441 cagccgcgag ctctacgcgc tggtggatcg gttgccggag gagcaggcga acctgatcca
     1501 gctccggctg gccgggttga ccgaccggga gatcgccggg gtgctcggcc gcagctatgg
     1561 cgcgatccgg gtggcccagc accgggcgat caaacgactc caggagctgg caacgaacgc
     1621 atccgcgacg aaaaggacac ggcagtgacg agaccagacc agcaccgcgc atcccctcat
     1681 gatccggacg cggatcgcct cagcgcgacc tggaacgacc ttgtcgccgg ccgtcagccc
     1741 gtcgaatccg acgaactgac cgatgccgtc caatcgctgc acgccgccgc gccgacctac
     1801 cgaccgacat cgaccttcag agacgagtta cgggagcaac tgatgcacac tgcaatgcca
     1861 atcgagatgc cgggagtccg gcagcagccg cgcctgcggg aacaaactgc cgagccccgg
     1921 ctgcgtgttc ccacggtcct gggcggccac gccgtccgct ggaccgcaat cgccgcaacc
     1981 atcgccctgc tgctgacgac cctggcggcg ggctacctcg gcggcctcct tccagccggc
     2041 aacggttcgg ggccgacccg gaacgtggcc gcgccatatc tggtgaatgg cacgccggta
     2101 gcgggcacgc ctgtctccgg aacaccggtc gccaacgtct gcgacacggt cgggcgatat
     2161 ctgccgtgcg gtccgaatcc agagatcggg cccggcatgt tcgatccctc ccggctgccc
     2221 gcagaggcgc tggatgtgac gaaggcgcag cttcagggct gggaagtaga gggatcgggg
     2281 acggtcacgt tctctgcccc gcacaatcca gttaccggtg tggcggtcga tgtggtggtg
     2341 atgggcgcat accgggcgaa attctccgcg ccggtcaccg tgtaccgcgc ctatccgggt
     2401 agcggcggcg tttggctgta tccggaggcg ggcgcacaga tcgagctgag ccggggcgat
     2461 tccgtctcgt atcagatcgg gacgaagaca gagctggaga atccattcaa tgcgtggacg
     2521 ctcaagttca agaccgcgct gttctacgat ggcgacccgt cgccgggtaa tttcggcgcg
     2581 gccggaaacg ttcaggtgcg tatcgatggc gatggcacat tgccgaagcc actcgccgaa
     2641 ctcggcgcgc cggagatcag cgtcgagatc agctacgcga ccttgccgga ggtcggcgca
     2701 cccatcagcg gtacgacgtt tctgacgccg gtgatcggcc cggtagccag tacgcaggtg
     2761 ctcggcggcc cgaatgaagg cttcgtcgtc tgggtctcga ccaacgcccc gcgcgggtag
     2821 cgagctcaga ccaaaccgaa cgaaagacgg ggcgttcagc aaaggacgcc ccgcttcgtg
     2881 cagcaatgcc ggccaccggg gctatgcttc gtccgaaaac cgaaaaccga aaaccgaaaa
     2941 ccgaaaaccg aaaaccgaaa accgaaaacc gaaaaccgac tacgactctt ccgccatgtc
     3001 gtccttgttc acggtcaggc gaacggtgtc gcccgagacg ccggcgatgc ggtcggcggc
     3061 gacatatcga tccttggcga agaggccctt ggaatcgatc ttgatgaagc cgacccgcat
     3121 cagacgggcg gccagggcgg gcgggagatc gggctcgccg tcccagccga agatttcggc
     3181 aaagttctcg gccagcccgt cccgggtatc ggcgtcggcg ccgatcgtgg cggcctgggg
     3241 atcgcccatc ttgacgaatt cgaccctgcc gatgtcgtcg ccggcggcat cgacgacgtg
     3301 cattcccttg tgaacctttt cgattgggcc ggcatcaccg gtgttggcga aggtatcgga
     3361 tgtgccgggt gggtttccgc gctccattgc agcattctcc cgtggtgggc tcgtgcaccg
     3421 ggcgatgttc gcccggccat cagccgttta gcctagcaag ggagcagcag cccacggtgg
     3481 tcggaggcta cgccggggtt gagggtgggg gtggagggag acggtgtagg ggcagatggc
     3541 atctgcccgc cgctcccccg aatgcccggt tgcccgtacc acgaccccgg tgtagcgcgg
     3601 tccccccgtg gaccgccccc gttcattccc ccgcacggtc gcatgcaccg aaccggcgcc
     3661 ggggttggtt cccgctcacg accgggtcag gatcgggtcc gggcggtcca cgggggaccg
     3721 tgctagatct tgggtcccgc tctccccagc cggcttcagc cgggttcaga tttcagccgg
     3781 ggtgtttacg cccgggcgat cccgggtacg aacgcccata tgccaccggg tcctcgggca
     3841 acgaagggca gatgccatct gcccctacac cttcttcgcg cctccatacc cctgatctgc
     3901 ccccgcagat ccctacagcg tcccgcctgc tcggctgctc gactcctcgc ctgctcgact
     3961 ccgctcgtac gctgcgcggc aatcggcgat cacctggcgg gtttcgtcga tgtcgtgcca
     4021 gccctggacg ttggtttgct tcggttcgag caatttgtag gtgtcgaaga aggtttcgat
     4081 ctcgcgcagc cagtgctggc cgagatcctc gagcgaattg acgtgggcga agcgcggatc
     4141 gccgaccggt acggccagta ccttgaaatc cgagcccttt tcatcggaca tgtccagccc
     4201 gccgatcgcg cggatttcga tcatgcagcc ggggaaggtc ggctcctcga ccaggatcaa
     4261 tacgtcgagg tggtcgccgt ccggcgcgag gg
//
LOCUS       DASSGX010000264         4275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_594382, whole genome shotgun sequence.
ACCESSION   DASSGX010000264 DASSGX010000000
VERSION     DASSGX010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4275
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594382"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /gene="ruvA"
                     /locus_tag="VFI12_04620"
     CDS             <1..570
                     /gene="ruvA"
                     /locus_tag="VFI12_04620"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HET7055723.1"
                     /translation="AGLRGEIAAKTDDAILVDVNGVIYQVRTSTNTLAETGPTGDPID
                     LRTILIVREDALTLYGFVTAEELRFFEVMTGVSGIGPRLACAILSTLKPDALYAAVQQ
                     GDVEMLATVPGIGRKTASRIIVELSGKLPESQFVQEDVLQDRDVLEALRAMGYSASEA
                     QQALSRAQITPGMTVEERLVAAFQQLGER"
     gene            complement(567..1898)
                     /gene="hisD"
                     /locus_tag="VFI12_04625"
     CDS             complement(567..1898)
                     /gene="hisD"
                     /locus_tag="VFI12_04625"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HET7055724.1"
                     /translation="MSDVIRILTDLSEVRAALTRSRGFEEPELSPQARAGVKRVFGEE
                     LTAGQVVDRVLERVRTEGDVGVAALTAAIDGQAPDQVEVPRDEWQSAFDRIDPALQQA
                     MRTAAAQIEAFHRHQVRTSWFETTTEGILGQLVRPIERVGVYAPGGTAVYPSSLLMTA
                     VPARVAGVSEVVVCSPPHNGAVSDLILAAAHVAGVDRVFQIGGAQAIAAMAYGTETVP
                     HVDKIYGPGNIFVVLAKQKLYGVAAIDALPGPTETLLIADDMADPVLAAADLLAQAEH
                     DPMASAILLTDSRTLADRVAVELERQLAELERSAIAAQSLKANGLIGIVPDLVTGVDL
                     ANAYAPEHLCLLTHSPWDLVPYVKHAGGLFIGEDSPEALADYTTGPSHVMPTGGSARF
                     FSPVHINDFVKVINVAAANRAALKRLGPATIALARAEGLTAHARAIERRLD"
     gene            complement(1891..4158)
                     /gene="pheT"
                     /locus_tag="VFI12_04630"
     CDS             complement(1891..4158)
                     /gene="pheT"
                     /locus_tag="VFI12_04630"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HET7055725.1"
                     /translation="MPDVQVLADVQAGEHRLTVVTGAPNIAAGQKVALALAGARLYDG
                     HSDSPTPEIKTLKPGTIRGIKSEGMVCSEKELGISDEHEGILVLPDDAPVGTPFVDYY
                     GDTVIEFEITPNLAHAFSINGIAREVHALLDVPVEQPPHLELSLIADAPDDVVVIADP
                     ERCNRYMVLLVDNLVVGPSPVWMVQRLEAAGMRSINNVVDMTNYVMHEFGFPMHAFDR
                     DKLAGGRVIVRAAEPGEHLETLDHIERQLTESMTVIADLEKPVGLAGIMGGFEAEVSG
                     ATTRLLLEVAHFDPTITRATSRALKLRTEASARYERGLDPAGIPTAVARAAQLIQELC
                     PGATLVGLADAYPAPVEQRAISFPFDRIEQLLGMRIGERTVLDILRRLEFNADIADGV
                     LSAVVPTYRRDVRSREDIIEEVARIAGYDLLPATLPSGEPQHVHRDPMYRLRKAARSV
                     LVGAGFNEAITYVTLENQDIDRFSDGATSGVVVESCRQRLIQLRNALQADRNILRPTL
                     IPSLLASLSANLNHEPGVRLAEIARVYAPHDGDVLPREVEVAGLVAAGRALPLGLDAT
                     DDQINFFDLKGTIALMLERLGVTGVLTERWSHPAFHPGRSAQMRSDNDILARFGELHP
                     EIATAYGIDDVRVIAGEVNLSAALSAIKPRGRDAVVPRFLPVQQDFAVVVQNDVPAAD
                     VEAAFRGGAGPLLTDITLFDQFRGPQIGEGNVSMAWRLTFAAPDRTLTDNDLVKIRPK
                     IEKTLKQRVGGVLRV"
BASE COUNT          707 a         1395 c         1463 g          710 t
ORIGIN      
        1 gccggcctgc ggggcgagat tgccgccaag acagacgatg cgatccttgt cgacgtcaac
       61 ggcgtcatct accaggtccg caccagcacg aacacgctgg ccgaaaccgg gccgaccggc
      121 gatccgatcg acctgcgcac aattttgatc gttcgtgaag acgcgctcac gctctacggt
      181 ttcgtcacgg cggaggagct gcgcttcttc gaggtgatga ccggtgtgtc gggcatcggt
      241 ccccggctag cctgcgcgat cctttcgacg ctcaagcccg acgctttgta cgcggccgtg
      301 cagcagggcg atgtcgagat gctcgccacg gtgcccggga tcggccggaa gacggcatcc
      361 cgcatcatcg tcgagctcag cggcaagctc ccggaatcgc aattcgtcca ggaagacgta
      421 ctccaggatc gcgacgtcct tgaggcactg cgggcgatgg gctactcggc gtccgaggcg
      481 caacaggcgc tttcccgggc gcagatcacg cccgggatga ccgtcgagga acggctcgtc
      541 gcggccttcc agcagctcgg cgagcgttaa tccagccgcc gctcgatggc gcgcgcgtgg
      601 gcggtcaggc cctcggcgcg ggcgagcgcg atcgtggcgg gtcccagccg cttcagcgcc
      661 gcccggttgg ccgcagcgac attgatcacc ttgacgaaat cgttgatatg caccggcgag
      721 aagaagcgcg cgctgccacc ggtcggcatg acgtggctcg gaccggtcgt gtagtcggcc
      781 agggcctcgg ggctatcctc gccgatgaac aggccgcccg cgtgcttgac atatggcacg
      841 agatcccacg gcgagtgcgt cagcaggcaa aggtgctccg gcgcgtaggc gttggccaga
      901 tccacgccgg tgacgaggtc cgggacgatc ccgatcagcc cattggcctt gagtgattgc
      961 gcggcgattg ccgaccgctc caactctgcc aattgccgtt ccagctcgac tgcgacgcgg
     1021 tccgccaggg tgcgcgagtc ggtcagcaag atggccgacg ccattggatc gtgctcggcc
     1081 tgtgcgagca ggtcggccgc ggcaaggacc gggtcggcca tgtcgtcggc gatcagcagg
     1141 gtttccgtcg ggccggggag cgcatcgatc gccgccacgc cgtacagctt ttgcttggcc
     1201 aggacgacga agatgttgcc aggcccgtag atcttgtcaa cgtgcgggac cgtctctgtg
     1261 ccatacgcca tcgccgcgat cgcctgcgcg ccgccgatct ggaagacccg gtcgactccg
     1321 gcgacgtgcg cggcggcgag gatcaagtcc gaaaccgcgc cgttgtgtgg cggcgagcag
     1381 acgacgactt ccgatacccc tgcaacgcgc gcgggaaccg ccgtcatcag gagcgacgac
     1441 gggtaaaccg ccgtgccgcc cggcgcataa acccccaccc gctcgatcgg tcggacaagc
     1501 tggccgagaa tgccctcggt cgtcgtctcg aaccacgaag tccggacctg gtggcggtgg
     1561 aatgcctcga tctgcgcggc ggcggtccgc atcgcctgct gcagcgcggg atcgatccgg
     1621 tcaaaggcgg attgccactc atcgcgcggc acttcaacct ggtcgggcgc ctggccatcg
     1681 attgcggccg tcagggcggc cacgccgacg tcgccttcgg tgcggactcg ctcgaggacg
     1741 cggtccacaa cttgtccggc cgtcagttcc tcgccgaaga cgcgcttcac gccggccctg
     1801 gcctgcggtg atagctctgg ttcctcgaag ccgcgcgaac gggtcagcgc cgctcgcacc
     1861 tcgctgaggt ctgtgaggat ccggatcacg tcagacacgg agcacccctc cgacgcgctg
     1921 cttgagcgtc ttctcgatct tcggccggat cttgaccaga tcgttgtccg tcaacgtccg
     1981 atccggcgcg gcgaaggtca ggcgccaggc catgctgacg ttcccttcgc cgatctgcgg
     2041 accccggaac tggtcgaaga gcgtgatatc cgtcaggagc gggccagcgc cgccacggaa
     2101 tgcggcctca acgtcggccg ccgggacatc gttctggacc acgacggcga agtcctgctg
     2161 gaccggcaag aagcggggta cgaccgcgtc ccggccacgc ggcttgatcg cggaaagcgc
     2221 ggcggacagg ttgacctcgc cggcgatcac ccgcacatca tcgatgccgt acgccgtggc
     2281 gatctcggga tgaagctccc cgaacctggc gaggatgtca ttgtccgaac gcatctgcgc
     2341 tgaacgaccc gggtgaaatg ccgggtgtga ccagcgctca gtcagcactc cagtaacccc
     2401 gagccgttcg agcatgagcg caatcgtgcc cttgaggtcg aagaagttga tctgatcgtc
     2461 cgtagcgtcg agaccgagcg ggagcgcccg gccggcagcg accaggccgg cgacttccac
     2521 ttcacgcggg aggacatcgc cgtcgtgcgg tgcgtagact cgcgcgatct ccgcgagacg
     2581 gacgcccggc tcgtggttca ggttggcgga gagtgaggcg agcaacgacg gaatcagtgt
     2641 cgggcggagg atgttgcggt cggcctgaag ggcatttcga agctggatca ggcgctgcct
     2701 gcatgattcc acgacgacgc ccgacgtagc gccatcagag aaccggtcga tgtcctgatt
     2761 ctcgagcgtc acgtaggtga tcgcttcgtt gaatcccgca ccaacgagaa ctgaccgcgc
     2821 cgccttccgc aggcggtaca tcgggtcgcg gtggacatgc tgcggctcgc ccgaggggag
     2881 cgtggcgggg agcaggtcgt accccgcgat ccgcgcgacc tcctcgatga tgtcctcgcg
     2941 actgcgcacg tcgcgccgat aggtcgggac aaccgcagac aggactccat cggcgatgtc
     3001 ggcgttgaac tcgaggcggc gcaggatgtc gagcacagtc cgctcgccga tccgcatacc
     3061 gaggagctgc tcgatccgat cgaacgggaa cgagatcgca cgctgctcga ccggcgccgg
     3121 ataggcatcg gccaggccca ccagtgtcgc gccggggcag agttcctgga tcaactgagc
     3181 ggcgcgagcg acggcggttg ggattccggc cggatcgaga ccgcgctcgt accgggcgct
     3241 ggcctcggtc cgcagtttca gggctcgaga tgtcgcccgg gtgatcgtcg gatcgaagtg
     3301 cgcgacctcg agcaggagtc gggtcgtcgc gccggacact tcggcctcga agccgcccat
     3361 gatcccggcc aggccgaccg gcttctcgag gtcggcgatg acggtcatgc tctcggtgag
     3421 ttgtcgttcg atgtgatcga gcgtctcgag gtgttcgccg ggctcggccg cgcggacgat
     3481 cacccgaccc ccggcgagct tgtcgcggtc gaaggcgtgc atcgggaagc cgaactcatg
     3541 catgacgtag ttcgtcatgt cgaccacgtt gttgatcgag cgcattccgg ctgcctccag
     3601 ccgctggacc atccagactg gcgacgggcc gaccacgagg ttgtcgacga gcaacaccat
     3661 gtaccggttg cagcgttccg ggtcggcgat cacgaccacg tcatcgggcg cgtcggcgat
     3721 cagcgacagt tcgaggtgcg gtggttgttc gacggggaca tcaagcagcg cgtggacttc
     3781 gcgggcaatc ccgttgatcg agaaggcatg cgccaggttc ggcgtgatct cgaattcgat
     3841 taccgtgtcg ccgtaataat cgacaaaggg cgttccgacc ggcgcatcat cgggcagcac
     3901 gaggatgcct tcgtgctcgt cggagatgcc gagctccttc tcggagcaga ccattccttc
     3961 ggacttgata ccgcggatcg tgcccggttt gagcgtcttg atctccgggg tcgggctgtc
     4021 cgagtggccg tcatagagcc gcgcgccggc cagcgccagc gccaccttct ggccggcggc
     4081 gatgttcggc gcgccggtga cgacggtcag gcgatgctcc ccggcctgga catcggcgag
     4141 gacctggacg tctggcatta cgccatcgcg acgggcgcga tgcaggagat cgcgcccggc
     4201 gcggccggct gaggccgcgt cccccgccgt agatccccgc tcgatacctc atgcggcgag
     4261 gggcggggcg gtagg
//
LOCUS       DASSGX010000265         4282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1357478, whole genome shotgun sequence.
ACCESSION   DASSGX010000265 DASSGX010000000
VERSION     DASSGX010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1357478"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(211..1317)
                     /locus_tag="VFI12_04635"
     CDS             complement(211..1317)
                     /locus_tag="VFI12_04635"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET7055726.1"
                     /translation="MNYQRMIMEVESPEQIGYDRVRVNLAESSMRDRSLHDLGFDPAT
                     LGDLLLNYGDHRGPETLRQEIAVDGCGALTAADVLVTVGAAGALFLTATALLGPGDRL
                     VVARPNYASNLETPRAIGADLAYLELDFDSGYQVDPDRLADLITPGTRLVSLTSPHNP
                     TGAVIPPDQLAEIVRVVERAGTWLLLDETYRELGDEIHPPASVLSDRVISVSSLSKTY
                     GVPGIRVGWVTCRNRDLMTTLLAAQEQAALAHSVLDLAVAEHVFAQRDRLLPDIRNHI
                     AEQRRIVADWIAGDDRIEWVEPKGGVVCFPRFADPGIDARAIYVQLQNDGIFLGPGWW
                     FEQEDRHMRLGFGYPTADELKTGLTAISAQLHTR"
     gene            complement(1314..2204)
                     /locus_tag="VFI12_04640"
     CDS             complement(1314..2204)
                     /locus_tag="VFI12_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055727.1"
                     /translation="MTDQGPVRRYERTAEQTHPAEPSRTIAVAERDAAIAGSGLSDGG
                     KALVAALLRTSRPRIFPGGGLGIEYGALLKTAARPDLLEPHIAEVVAHLRSAQIDILF
                     VPGMSGYPIGAMYSQASGIPALLLKKQPYPATPETELVPGAFVIPSYTGAGDTLISAD
                     TAAAADILAGIIDRQLAEQADSPWVQLVIRIAGADEIIDKATMATAITETAPVFCQSV
                     IEDVLVARRERIGQRPVAVGISVDAWVTPLLKGYNGAAGILQSRCGITPFAGVTITSL
                     HVDPPAIGIEGIGVLACEEP"
     gene            2241..2453
                     /locus_tag="VFI12_04645"
     CDS             2241..2453
                     /locus_tag="VFI12_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055728.1"
                     /translation="MLQKPDDEHIDLDVNPVADLLDDDEFTDVDPYGEPPVELTELPG
                     WVRALIIIVVVAMVLSVTWWIVPALR"
     gene            complement(2461..3405)
                     /locus_tag="VFI12_04650"
     CDS             complement(2461..3405)
                     /locus_tag="VFI12_04650"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7055729.1"
                     /translation="MDSVWSIAFLVAVLSAAIPAGTAILYACLGELLSERSGVLNLGV
                     EGMMLMGALGGVAVTIWTGSAWIGVLGAILAGAAMASLHAIVTVGLRANQVVSGLALT
                     LLGTGLSAFLGRNLVGKPLPSKFGDVSIPLLGDIPKLGDILFDQSPLVYLSFVVAPVM
                     WWYIFRTQPGLHLRSIGENPAAADAMGVPVNWLRFLYVIIGGGFAGLGGAVISLSTNP
                     GWTEGMTAGRGWIAVALVIFAGWNPAWAVAGAYLFGGVQAGQFQLQTVGVGISPFFLN
                     MLPYLFTVIVLVIASGDFARKRIGAPAALGRPFLREER"
     gene            complement(3398..>4282)
                     /locus_tag="VFI12_04655"
     CDS             complement(3398..>4282)
                     /locus_tag="VFI12_04655"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7055730.1"
                     /translation="GLAVAIAFRMQLWNIGAEGQLYMGAVAAAGTALFLLPDARGLVL
                     IPAMLIAGAIGGAFWGGIPGVLRAKFNANETITTLMLNYVAILFTQYLVHGPWRDPQA
                     FGFPGTKSFPDAAALPHYGTYRVHLGLVFGLVAAVILLAVLRRTRFGYELDVIGDNPR
                     AARYVGMRTTRMIIIVMAISGALAGIAGMSEVAGIGHSLQRSISPGYGYTAIIVAWLA
                     RLNPIAIIAVSFLMSVILVGSDQLQTAIGLPGSVGPMLQGAILFFLLAGEFLTNFRVH
                     IRRPMVETIDAEPEPARG"
BASE COUNT          740 a         1345 c         1584 g          613 t
ORIGIN      
        1 gtgcctgata ccgcccccga tgtaggggtc cgcggggggc ggacccgccg ttcccgaatc
       61 gcgcggtgtg tgatgggaag cgcgcagatc agcgtggttc cgaacgcgac cgggttcacc
      121 ggcgaggcgg gtatgccccc acacgcccct gcacggccga cgccatccat ggacgaattc
      181 agcaggaggt gggtttgcgg cagcgcatcc ctaccgggtg tggagctggg cggagatggc
      241 ggtgaggccg gtcttcagct catcagcggt ggggtagccg aagccgaggc gcatgtggcg
      301 gtcctcctgc tcgaaccacc agccggggcc gagaaagatg ccatcgttct gcagctggac
      361 gtagatggcg cgggcgtcga tccctgggtc ggcgaagcgg gggaagcaga cgacgccacc
      421 tttcggctcg acccactcga tgcgatcgtc accggcgatc cagtcggcca cgatccggcg
      481 ctgttcggca atgtgattgc gaatatcggg cagcaggcga tcccgctggg cgaagacgtg
      541 ctccgccacc gcgagatcaa gcacggaatg ggcgagcgcg gcctgttcct gggcggccag
      601 cagggtcgtc atcaggtcgc gattccggca ggtgacccag ccgacgcgga tgccggggac
      661 gccgtaggtc ttcgaaaggg acgagacgct gatcacgcgg tcgctcagga ccgaggccgg
      721 cgggtggatc tcgtcgccga gctcgcgata ggtctcgtca aggagaagcc aggtgccggc
      781 ccgctcgacc acccggacaa tctccgcgag ctgatcgggc ggaatgaccg cgccggtcgg
      841 gttgtgcggg ctggtcaggc tgacgagcct ggtgccgggc gtgatcaggt cagcgagacg
      901 atcggggtcg acctggtagc cggaatcgaa gtcgagctcg agataagcga ggtcggcgcc
      961 gattgcgcgg ggtgtctcga ggttgctggc gtagttcggg cgggcgacga cgagacgatc
     1021 gccgggaccg agcagcgcgg ttgcggtcag gaagagggcg ccggccgcgc cgaccgtaac
     1081 cagcacgtcg gcggcggtca gggccccgca tccgtcgacg gcgatttcct gccggagcgt
     1141 ttccgggccg cggtgatcgc catagttcag caaaaggtcg ccgagcgtgg ccggatcgaa
     1201 gccgaggtcg tggagagagc gatcccgcat cgagctttcg gcgaggttga cgcgaacacg
     1261 gtcgtagccg atctgctcgg ggctctcgac ctccatgatc atgcgctggt agttcatggc
     1321 tcctcgcagg cgagcacgcc gatgccctcg atcccgatcg cgggcgggtc gacgtggagg
     1381 ctggtgatgg tgacgccggc gaagggggtg atcccgcagc gggattggag gatcccggcc
     1441 gcgccgttgt aacccttgag cagcggcgtc acccaggcat cgaccgaaat accaaccgcg
     1501 acgggccgct ggccaatccg ctccctacgc gcgaccaaca cgtcttcgat cacggactgg
     1561 cagaagaccg gcgcggtttc ggtgatcgcg gtcgccatgg tggccttgtc gatgatttcg
     1621 tcagcacccg cgatgcggat cacgagctgg acccagggcg aatccgcctg ctcggccagt
     1681 tggcggtcga tgatcccggc caggatgtcg gccgcggcgg cggtgtcggc cgagatcaga
     1741 gtatcgccgg cgccggtgta ggacgggatg acgaaggcgc cgggcacgag ctcggtctcg
     1801 ggcgtggccg ggtagggttg cttcttgagc aggagcgccg ggatgccgct ggcctggctg
     1861 tacatcgcgc caatcgggta accggacatg cccggcacga agaggatgtc gatctgcgcg
     1921 cttcgcaggt gcgcgacgac ctcggcgata tggggctcga gcaagtcggg ccgggccgcg
     1981 gtcttgagca gggcgccgta ctcgatcccg agtccgccgc cgggaaagat ccggggccgg
     2041 ctggtccgga gcaatgccgc gaccagcgcc ttgccgccat ccgagagccc gctcccggcg
     2101 attgcagcat cccgctcggc gaccgcaatc gtccggctcg gctcggcggg gtgcgtctgc
     2161 tcggcggttc gctcgtagcg gcgcacgggt ccctgatcgg tcatggcgta ctgttgcccg
     2221 aatagggagg gggtttgctg ttgctccaga agcctgacga cgagcacatc gacctggacg
     2281 tgaatccggt cgcggacctg ctcgacgatg acgagttcac cgatgtcgat ccgtacggtg
     2341 aaccgcccgt ggagctgacc gaactgccgg gatgggtgcg cgcgctgatc atcatcgtgg
     2401 tcgtggccat ggtgctgtcg gtcacgtggt ggatcgtgcc ggcgctcagg taggacatga
     2461 tcaccgctcc tcgcggagga agggacgacc gagcgcggcc ggggcgccga tccgcttccg
     2521 ggcgaaatcg ccggaggcga tcacaagcac aatgacggtg aacaggtacg gcagcatgtt
     2581 caggaagaag ggcgagatgc cgaccccgac cgtctgaagc tggaactggc cggcctgcac
     2641 gccgccgaag agataggccc cggcgaccgc ccaggccgga ttccagccgg cgaagatgac
     2701 cagcgcgacg gcgatccagc cgcgcccggc ggtcatcccc tcggtccagc ccgggttcgt
     2761 gctgagcgag atgaccgcgc cgccaagtcc cgcgaagcca ccgccgatga tcacgtacag
     2821 gaagcggagc cagttgaccg gcacgcccat cgcatcggcc gcggccgggt tctcgccgat
     2881 cgagcggagg tgcaggccgg gctgggtgcg gaagatgtac caccacatga ccggcgcgac
     2941 cacgaacgag aggtagacga gcggcgactg gtcgaagagg atgtcgccca gcttcgggat
     3001 atcgcccagc agcggaatcg agacgtcgcc gaacttgctc ggcagcggct tcccgacgag
     3061 gttgcggccg aggaaggcgg agaggccggt accgagcagg gtcagggcga gaccgctgac
     3121 gacctggttg gcgcggaggc cgaccgtgac gatcgcgtgg agcgaggcca tcgccgcccc
     3181 ggccaggatc gcgccgagca cgccgatcca ggcgctgccg gtccagatcg tgaccgcgac
     3241 gccgccgagc gcgcccatca gcatcatccc ctcgaccccg aggttgagga cgccggagcg
     3301 ttcgctgagc agctcgccga ggcaggcgta gaggatcgcg gtcccggccg ggatcgccgc
     3361 cgagaggacg gcgaccagga aggcgatcga ccagacgcta tccacgggcc ggctccggtt
     3421 ccgcatcgat ggtctcgacc atgggacggc gaatgtggac gcggaagttg gtcaggaact
     3481 cgccggccag caggaagaag aggatcgcgc cctggagcat cggcccgacc gacccgggca
     3541 ggccgatcgc ggtctggagc tggtcgctgc cgacgaggat gaccgacatc aggaagctga
     3601 cggcgatgat cgcgatcgga ttcaaccggg cgagccaggc gacgatgatg gcggtgtagc
     3661 cgtacccggg cgagatcgag cgctggagcg agtgaccgat gccggcgacc tcgctcatcc
     3721 cggcgatccc ggccagcgcg ccactgatgg ccatgacgat gatgatcatc cgggtcgtcc
     3781 gcatgccgac gtagcgggcg gcgcgcgggt tgtcgccgat cacgtcgagc tcatagccga
     3841 agcgggtccg gcggaggacg gccagcagga tgaccgccgc gacgaggccg aagaccaggc
     3901 cgaggtgaac gcggtaggtc ccgtagtgcg gcaaggcggc ggcgtccgga aacgatttcg
     3961 tgccggggaa accgaaggcc tgcgggtcgc gccaggggcc atggacgagg tactgcgtga
     4021 agaggatcgc gacgtagttg agcatcagcg tcgtgatcgt ctcgttggca ttgaactttg
     4081 cccggagcac gcccgggatc ccgccccaga aggccccgcc gatggccccg gcgatcagca
     4141 tggccgggat caggaccagg ccgcgcgcat ccggcagcag gaaaagggcc gtgcccgccg
     4201 cggcgaccgc acccatatag agctggccct cggcgccgat gttccagagc tgcatccgga
     4261 aggcgatcgc caccgcgagg cc
//
LOCUS       DASSGX010000266         2090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_485131, whole genome shotgun sequence.
ACCESSION   DASSGX010000266 DASSGX010000000
VERSION     DASSGX010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485131"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..544
                     /locus_tag="VFI12_04660"
     CDS             212..544
                     /locus_tag="VFI12_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055731.1"
                     /translation="MRADVRLRSDGRTVTGKLPEGRRFGVSEGRTERIERMVRSEYPD
                     GTVVDSEPVEVAQGAVPTWAGIAGMFTRMKEPPGTSRRVFTRRVTVEAGPWTEVVVPT
                     DGPAEGRA"
     gene            541..879
                     /locus_tag="VFI12_04665"
     CDS             541..879
                     /locus_tag="VFI12_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055732.1"
                     /translation="MKALVIGGERHGEFVDVLDGAQVWVDIEHAVTHRMRRITNTITD
                     AQGHVKEVYTLDLAVHEGMLGPQELNYTMASLSTLAMNAFTREHGTAQEVPQEPAGSS
                     LIVPGQDNKP"
     gene            876..1256
                     /locus_tag="VFI12_04670"
     CDS             876..1256
                     /locus_tag="VFI12_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055733.1"
                     /translation="MIDLVARLRHAWDERERQLAEDDRVARAAIADDGGLDGGFENAT
                     WLTDVRLIKPRFGDDAAAMIRRFAVPRRVLAEVERERRDLAAKRRILDRVEPEIDDGT
                     SGSRIARFTIRALAEAENLVEVVS"
     gene            1253..1720
                     /locus_tag="VFI12_04675"
     CDS             1253..1720
                     /locus_tag="VFI12_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055734.1"
                     /translation="MTERMTGTDRTGDPLLSLDEACIALRQLLISSQWYSTYLFGMPG
                     APIGERWHRRYREPRPGELCFVTDHAINPRADDDTIVKSFGYFLGEQQEPIYDWDTWV
                     MVQDQYDDMVECPTERVFYIQYGPAPDDVCRWANADHQVIATQAISDELHNRC"
     gene            1722..2006
                     /locus_tag="VFI12_04680"
     CDS             1722..2006
                     /locus_tag="VFI12_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055735.1"
                     /translation="MAPAFGSLVIDEWIFPVTGVRLTGGKVRIAAEIEGPTPEISLNT
                     YVVQGDDGRTVYQSSRGESIPPVEEGWTMTIILDLEMEGLVRPAGPILIT"
BASE COUNT          365 a          664 c          752 g          309 t
ORIGIN      
        1 aggccgatgc cgacatcggc acgtggcgac ccgaagacct acgttccggt cgcatcgagc
       61 gcgagttgcg gacgctccgc aagccgcaat cggtacggtc cggcataccg caacggcccc
      121 gcaagggact gtccgcaatc ccgcagacct gaccgcaagt acagtgcgac cagcagaaac
      181 aggagatgat gaccatgacg aaggaatgcc cttgcgagcc gatgtccggc tgcgttccga
      241 tgggcgcacg gtgaccggca agctgccgga gggcaggcgg ttcggcgtgt ccgagggccg
      301 caccgagcgg atcgagcgga tggtccggtc ggagtacccg gacggcacgg tggtcgacag
      361 cgagccggtc gaggtcgccc agggtgcggt gccgacgtgg gcgggcatcg ccggcatgtt
      421 cacgaggatg aaggagccgc cgggcacttc gcgccgggtc ttcacccggc gggtaacggt
      481 tgaggcggga ccctggaccg aggtggtcgt cccgacggac ggcccggcgg agggtagggc
      541 atgaaggcgc tggtcatcgg cggggagagg cacggcgagt tcgtcgacgt cctggacggc
      601 gcgcaggtct gggtggacat cgagcacgcg gtgacgcatc ggatgcgccg gatcacgaac
      661 acgatcaccg acgcacaggg ccacgtgaag gaggtctaca cgctcgatct ggccgtgcac
      721 gagggcatgc tcggcccgca ggaactcaat tacacgatgg cgtcgctgag cacactggcg
      781 atgaacgcgt tcacccgtga gcacggcacg gcgcaggagg tcccgcagga gccggccggc
      841 tcctcgctga tcgtgcccgg tcaggacaac aagccgtgat cgacctggtg gcgcggctgc
      901 gtcacgcctg ggatgagcgg gaacgccagc tcgccgagga cgatcgggtg gcgcgggcgg
      961 ccatcgcaga cgacggtggc ctggatggcg gcttcgagaa tgcgacgtgg ctgaccgacg
     1021 ttcggctcat caagccccgc ttcggcgacg acgcggccgc tatgatccgg cgcttcgccg
     1081 ttccgcgccg cgtgctggcc gaggtggagc gggagcggcg cgacctcgcc gccaagcggc
     1141 gcatcctcga ccgcgtcgag cccgaaatcg acgacggcac cagcggatcg cggatcgccc
     1201 ggttcaccat ccgtgcgctc gccgaggctg aaaacctggt cgaggtggtc tcgtgaccga
     1261 gcggatgacc ggcaccgacc ggacgggcga tccgctgctc agcctggacg aggcgtgcat
     1321 cgcgctgcgc cagctgctga tctcgtcgca gtggtactcg acctacctgt tcggcatgcc
     1381 gggcgcgccg atcggcgagc gctggcaccg ccgctaccgc gagccgcgcc cgggcgagct
     1441 gtgcttcgtc accgatcacg cgatcaaccc gcgggccgac gacgacacga tcgtcaagtc
     1501 gttcggctac ttcctcggcg agcagcagga gccgatctac gactgggaca cgtgggtcat
     1561 ggtgcaggac cagtacgacg acatggtcga gtgcccgacc gagcgggtct tctacatcca
     1621 gtacggccca gccccggatg atgtgtgccg gtgggcgaac gccgatcatc aggtgatagc
     1681 cacccaggcg atcagcgacg agctccacaa tcggtgctga ggtggcgccc gcgttcggct
     1741 cgttggtcat cgacgagtgg atcttccctg tcaccggcgt gcgcctgacc ggtggcaagg
     1801 tgcggatcgc cgccgagatc gagggcccga ctcccgagat cagcctgaat acctacgtgg
     1861 ttcagggcga cgacggccgc acggtgtacc agtcttcacg tggcgagtcg atcccgccgg
     1921 tggaagaggg atggacgatg acgatcattc tggatctaga gatggaggga ctggttcgcc
     1981 cggccggtcc gatcctgatc acgtagcatt aaggcgactg ctcggtacga acacgactgg
     2041 ccccgcgacc gatgaggcgc ggggcttcgt cgtgccatga tctggtgcca
//
LOCUS       DASSGX010000267         5448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1031273, whole genome shotgun sequence.
ACCESSION   DASSGX010000267 DASSGX010000000
VERSION     DASSGX010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5448
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031273"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VFI12_04685"
     CDS             <1..409
                     /locus_tag="VFI12_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HET7055736.1"
                     /translation="IFDEPVYNYTRDFPYLWEEITLPIPYTADRAAAEQIMREAAERH
                     TGELATQSAAVLARLARAYSLQSSSTEPQVYYQLARDRLELTVRFVVDARGGRDTKDL
                     ISRDILNDFDAAGIAINPETPAPIVTASNPPPR"
     gene            complement(381..2348)
                     /locus_tag="VFI12_04690"
     CDS             complement(381..2348)
                     /locus_tag="VFI12_04690"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET7055737.1"
                     /translation="MRTRARIVTAAIVLLACILPLLRATAQNQPPGTPEAIERPTATS
                     TPEVASPAAVENAIPTSTELPDAASPAAAAETPGTALLTPTAVPPPLSCQVSKTRGYV
                     GTHVDFSCTGGDRARPLYVFLNGNRFFALSPTGDSTRSGTVRIPAYWAGTWRFSISDK
                     TVATWRTQFTIVPHFWVKPSSGPIGTKVLVKLAGFPALEQITLRFVDSGGKTIHFATV
                     STMHNGSASATWFINGSMRPGVGHFEAITGGTIVTAGAVQVASVQILTRRAFTVTERP
                     TATSTSTTTPTPTASNTPIATNTPTPTPTPTRTPTSPAGGSAVIVAAGDIACGAASTG
                     SSCKQKATSDLVVAQAPSAVLVLGDNQYECGETADWNAFYDPSWGRAKGITHPAIGNH
                     EYSTSPENPSCSSSVAGAPGYWSYFGAAATPRQPGCRVGCGGYYSYDVGSWHLIALNS
                     TCSQSGGCGAGSAQEQWLRADLAAHTNACTLAYMHHPRFSSGQIGNLAAMQPLWQALY
                     DSGADVVLVGHDHDYERFAPQTPGGALDDSFGIREFVVGTGGRNHTSFAAIQPNSEVR
                     NDSAFGILTLTLRSTGYDWRFLPISGSSFSDSGSGNCHGKPTAGMRGDNTVATTSRSI
                     FGLAQAPVLLGLLCMVTVRRRRSTVAAGSRQ"
     gene            complement(2509..2991)
                     /locus_tag="VFI12_04695"
     CDS             complement(2509..2991)
                     /locus_tag="VFI12_04695"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7055738.1"
                     /translation="MPTPLITLRPALVDEADALSALTRRSKQSHGYSDEFMERFMSQG
                     DMVITPESITRNPVMVAELDGRIAGFAHLKPINQPDAVCLEDLFIEPDAQGRGIGRVL
                     FDWALAEAARRGFAWLEWESDPNAAGFYEKLGAEKIGDSKSTVIEGRVLPMYRLRTRR
                     "
     gene            complement(2994..3878)
                     /locus_tag="VFI12_04700"
     CDS             complement(2994..3878)
                     /locus_tag="VFI12_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055739.1"
                     /translation="MSDLVPVPSEHAGEYARLLIEIETRKRVIGTLQTERDGLEMTLT
                     NFAVELKSRVGGLRVELNRVKRQVAEYRRRIERLRESDLLDPEQVEREVAEEFAEQLE
                     QERLAEEQAAREGQRIDVERPRPQLDAETEAEILRLYRELAKRFHPDRARTEEDRARR
                     TDLMLRINVAYSERDLVTLQAMARRADTGESAAVLLSDEERVQWAHRVIARMILQITE
                     LSEQIEMLRRSETYQMWKSPEDSSQSIQQVERRVRDRLNRERDRLDEAIIDYNRIVRR
                     RRLSHISVEREYAGSPGE"
     gene            4131..>5448
                     /locus_tag="VFI12_04705"
     CDS             4131..>5448
                     /locus_tag="VFI12_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055740.1"
                     /translation="MRRFLTALTLVLAIIPGPTAHAQSSRLAPSSFRQAPAGACGPDA
                     PDGLILHQAFAPDGSLADPPLALFNPTTNESGPVDVPDPREWLPMQYGCNAIVKTATG
                     AYYIVFGSTGESFRLDFLKPEEEWKFDTTAYWGTDGERRFGVFKTSAISGEQAILMDF
                     EQGIYWNLAPLITDDGSMQDASFSQSGDYLVVEGGPVLDQRTWLLPTSDPGQGRQIAF
                     PQFSNFHEFDEAEGRMLYWSVGAGEGEQLIVEEIDSADWYLVATDPEGKDEYDPYLSG
                     WFIPGHDDLIAVLHGDRFALVDISGPDPVEQFVIEGHRPFSNFYVAPSGKYGILDRKP
                     ATIFVDLETGEWREVTTEDGTAINSVDPYGRWGIATIGMVDEPPRGIGVIDLETAEFR
                     TVYMFGEGEKDDYNGTAWSRDGAVGLVTIVDANQQRHLSLISNRRGT"
BASE COUNT          900 a         1742 c         1854 g          952 t
ORIGIN      
        1 catcttcgac gagccggtct acaactacac gcgggatttc ccatacctct gggaagagat
       61 caccctgccc atcccgtaca ccgccgatcg cgccgcggcc gagcagatca tgcgcgaggc
      121 tgccgagcgc cacaccgggg agctcgccac gcagtccgcg gcggtactcg cccggttggc
      181 gcgggcctat tccctccagt cgagctcgac cgagccgcag gtctattacc agctggccag
      241 ggatcggctg gagctgaccg tccgcttcgt cgtcgatgcc cgcggcgggc gggacaccaa
      301 ggacctgatc agccgcgaca tcctcaacga cttcgacgcc gccggcatcg ccatcaaccc
      361 cgaaaccccc gcgccgatcg tcactgcctc gaacccgccg ccacggtaga gcggcgccgg
      421 cggaccgtca ccatgcagag gagcccgagc aataccggcg cctgcgccag cccgaatatg
      481 ctccgggacg tggtggcaac cgtgttatca ccgcgcatgc cggcggtggg cttaccgtgg
      541 caattgcctg agcccgaatc gctgaagctg gatccggaga tcgggaggaa ccgccagtca
      601 tagccggtcg accgcagggt caacgtgagt atcccgaagg cgctgtcgtt gcggacctcg
      661 ctattgggct gaattgcggc gaaggacgtg tgattgcggc cgccggttcc gacaacgaac
      721 tcgcgaatcc cgaagctgtc atcgagcgct cccccgggcg tctgcggggc gaagcgctcg
      781 tagtcgtggt catgcccgac cagcactacg tcggccccgg agtcgtagag ggcctgccag
      841 agtggctgca tggccgcgag gttcccgatc tggccggagc tgaagcgggg atggtgcata
      901 taagcgagtg tgcacgcgtt cgtgtgagcg gcgagatcgg cccgaagcca ttgctcctgt
      961 gctgatcctg ctccacatcc acccgactgg gaacaggtgg agttaagggc gatgaggtgc
     1021 caggagccga catcgtagct gtagtaaccc ccgcagccaa cgcggcagcc gggctggcgg
     1081 ggcgtggcgg cagctccgaa atagctccag tacccggggg cgccggcgac tgagcttgag
     1141 cagctcgggt tctcggggct ggtcgagtac tcgtggttgc cgatcgcggg atgcgtgatt
     1201 cccttggccc gtccccagga ggggtcgtaa aaggcgttcc agtcggccgt ctctccgcat
     1261 tcatactggt tgtcgccgag gacaagcacg gccgagggcg cctgggcgac caccagatcc
     1321 gaggtggctt tctgcttgca gctgctcccg gtgctggcgg cgccgcaggc aatatcgccc
     1381 gcggccacga tcacggccga gccaccggcc ggtgaggtcg gcgtccgggt cggcgtgggc
     1441 gtgggcgttg gtgtgttggt tgcgatcggg gtgttgctcg cggtcggggt aggcgtcgtg
     1501 gtcgaggtgg acgttgcggt cgggcgctcg gtcacggtaa atgctcggcg cgtgaggatc
     1561 tggacgctcg ccacctggac cgcacccgcg gtgacgatcg tgccgccggt gatcgcctcg
     1621 aagtggccca caccgggccg catcgagcca ttgatgaacc acgttgcgct cgcgctgcca
     1681 ttgtgcatcg tgctgaccgt ggcgaaatga atggtcttgc cgccagaatc gacgaagcgc
     1741 agggtgatct gctccagggc ggggaaaccg gccaatttga cgagcacctt cgtcccgatt
     1801 ggtccggatg acggcttgac ccagaagtgg ggcacgatcg tgaactgcgt gcgccaggta
     1861 gcgaccgtct tgtccgatat cgagaaccgc caggtccctg cccagtaggc cgggatacgg
     1921 accgtgccgg atcgggtcga gtcgcccgtc ggggaaagcg cgaagaagcg attaccgttc
     1981 aggaagacgt acagcggccg ggcccggtca ccacccgtgc aactgaagtc gacgtgcgtg
     2041 ccgacgtagc cgcgggtctt actgacctgg cagctcaggg gaggcggtac ggcagtcggc
     2101 gtcagcaggg cggtgcctgg cgtctcggcg gccgcggcgg gggaggccgc gtctggcagt
     2161 tcggtcgatg tcgggatcgc attctcgact gcggccggtg atgcgacctc aggcgtgctc
     2221 gtcgcggttg ggcgctcgat tgcctctgga gtcccgggcg gctggttctg cgcagtggcc
     2281 ctgagcagcg gcaatatgca ggcaaggaga acgatcgcgg cggtcacgat gcgggcgcgg
     2341 gtccgcatgg cggagctccg atctggcgga gggatgtcac gatcccgccc atcgcttgac
     2401 gctgtgtcac tttatggtat gtgcccggtc aataggtatg gttcatgttc tgctaccaag
     2461 gcagcgccgg gcgaagcgtg gccagcaggg accagggaac tgccgggatt agcggcgggt
     2521 tcgcagccgg tacatgggga ggacgcggcc ctcgatgacg gtggatttcg agtcgccgat
     2581 cttctccgcg ccgagcttct cgtagaagcc ggccgcgttc gggtccgatt cccattccag
     2641 ccacgcgaat ccccgccggg cggcttctgc cagcgcccaa tcgaagagga cacggccgat
     2701 gccccggccc tgcgcgtccg gctcgatgaa aaggtcctcg aggcacacgg cgtccggctg
     2761 attgatcggc ttgaggtgcg cgaatccggc tattcggccg tccagctcgg ccaccatgac
     2821 cgggttccgc gtgatcgact cgggcgtaat taccatgtcg ccctggctca tgaaccgttc
     2881 catgaactca tcgctgtagc cgtgcgattg cttggagcgg cgagtcagcg cgctcagcgc
     2941 gtccgcttcg tcgaccaggg ccgggcgcaa ggtgatcagc ggcgtgggca tcgctattct
     3001 cccggtgacc cggcgtactc gcgctcgacc gagatgtgag agagccggcg gcggcgcacg
     3061 atgcggttgt aatcgatgat cgcctcgtcc agccggtccc gctcgcggtt gagccggtca
     3121 cgcacgcggc gctcgacctg ctggatgctc tggctgctgt cctcgggcga tttccacatc
     3181 tggtaggtct cgctccggcg cagcatctcg atttgctcag acaactcggt gatctggagg
     3241 atcatccggg cgatcacccg gtgcgcccat tgcacccgct cctcgtccga gagcaggacg
     3301 gcggccgatt caccggtgtc ggctcgccgg gccatcgcct ggagcgtgac aaggtcccgc
     3361 tcgctgtagg cgacgttgat ccggagcata aggtcggtcc ggcgggcgcg atcttcctcg
     3421 gtgcgggcgc gatcggggtg gaaccgcttg gccagttcgc ggtagagccg gaggatctca
     3481 gcctcagtct cggcgtcgag ctgcggccgc ggccgctcga catcgatccg ctggccctcg
     3541 cgcgcggcct gctcctcggc cagccgctcc tgctcgagct gctcggcaaa ctcctccgcc
     3601 acttcgcgct cgacctgttc cggatcgagc agatcgctct cgcggagtcg ctcgatgcgg
     3661 cggcgatact cggcaacctg gcgcttgacc cggttcagct cgacccgcag cccgccgacg
     3721 cggctcttga gctcgaccgc gaaattggtc agcgtcatct cgaggccgtc gcgctcggtc
     3781 tggagcgtgc cgatgacccg cttgcgcgtc tcgatctcga tcaggagacg agcatactcg
     3841 ccggcgtgtt cgcttgggac agggacaaga tcgctcatgc agtggcggcg cggtggccgg
     3901 tcctcgcttt gctcgggcac ttccagccac ggtcgggatc gaccagtcag ccgggaaaat
     3961 aatcagcccc ctattgtccc acgccggagc ctgagaatcg acgtttattg acgagctggg
     4021 ctggattcgc ggagtgaggg tagcccatcg gacgctattg cgttaccgcg cataagcaat
     4081 acactgggcg aaacccgtgc ggtttcgaga cgaataggga ggaagcgcct atgcggcggt
     4141 ttctgacggc cctgaccctc gtgctggcaa tcatcccggg cccgaccgcc catgctcaat
     4201 catccagact ggcgccctcg tcgttccggc aggcgccggc gggtgcatgc ggccccgatg
     4261 ccccggacgg cctcatcctg caccaggcat tcgcgccgga cggcagcctg gccgatccac
     4321 ccctggctct cttcaatccc accaccaacg aaagcggtcc ggtcgatgtc cccgacccgc
     4381 gcgagtggct cccgatgcaa tacgggtgca acgcgatcgt gaagacggcc accggggcgt
     4441 actacatcgt ctttggcagc accggcgagt cgttccgact cgacttcctg aagcctgagg
     4501 aggagtggaa gttcgatacg acagcctact ggggcaccga cggcgagcgg cggttcggcg
     4561 tgttcaagac ctcggccatt tcgggggaac aggccatcct gatggacttc gagcagggga
     4621 tctactggaa cctggcaccg ttgatcaccg atgacggcag catgcaggac gccagctttt
     4681 cccaatccgg cgactacctc gtggtcgagg gcggcccggt gctggaccag cgaacgtggc
     4741 tgctgcccac gagcgatccc ggccagggac gccagatcgc atttccgcag ttctcaaact
     4801 tccacgagtt cgatgaggcc gaggggcgga tgctctactg gagcgtcggc gctggggaag
     4861 gcgaacagct cattgtcgag gagatcgact ccgccgactg gtacctggtc gcgaccgatc
     4921 cggaagggaa ggatgaatac gatccgtacc tctcgggttg gttcatcccg ggccatgatg
     4981 atttgatcgc cgtcctgcat ggggatcgct tcgccctggt cgacatcagc gggccggatc
     5041 cggtcgagca gttcgtgatc gaggggcacc ggcccttctc caatttctac gtcgccccga
     5101 gcggcaagta cggcatcctg gaccgcaagc cggccacgat cttcgtcgac ctcgagaccg
     5161 gcgagtggcg cgaagtcacg actgaagacg ggacggcgat caactcggtc gatccgtatg
     5221 gccgatgggg catcgccacg atcgggatgg tcgacgagcc gccgcgcggc atcggcgtga
     5281 tcgacctcga gacggccgaa ttcagaacgg tgtacatgtt cggcgagggc gagaaggacg
     5341 attacaacgg cacggcgtgg tcccgagacg gcgcggtcgg gctggtcacg atcgtcgacg
     5401 cgaatcagca gcgccacctg agcctcatca gcaacaggcg gggcacga
//
LOCUS       DASSGX010000268         3356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1248098, whole genome shotgun sequence.
ACCESSION   DASSGX010000268 DASSGX010000000
VERSION     DASSGX010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1248098"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..732)
                     /locus_tag="VFI12_04710"
     CDS             complement(76..732)
                     /locus_tag="VFI12_04710"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7055741.1"
                     /translation="MAEIRWFGHNCFRIRAKEATVIIDPVGKNTGYAMAKQTADIVLL
                     SHDHPGHANLAGVKSGYQIVEGPGEYEMHDVFITGIRTYHDGVKGTERGYNTAYLLEV
                     EGMKFCHLGDLGHMLNEDKLEVLNSCDILMIPVGGGDVITTEQAAELIGHLEPKVVLP
                     MQYATAIGDKKLATLEPFTKALGVTIPEAEEKLTLRSSDLSDSMRLVVLTPEAEAARR
                     "
     gene            1520..2425
                     /locus_tag="VFI12_04715"
     CDS             1520..2425
                     /locus_tag="VFI12_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055742.1"
                     /translation="MPPPAIGYDPDYSPGQPLTPLRGPRGANIVGPLLAVAALAIIVA
                     AVAFIVSQFRGGDTNNPQPTAVVAVNASPTAETDQQANTGAVDTSGGTGTNDPAAQPT
                     SETDSSEPTNTPRVNRPTETGDLSGSSDSNGSDGSTAPADRTRARQWLPTTKTVGSGY
                     QQTDNGTRDAAQVANSFPNPDEATTNLTEFGWAENAYREFSLPDGSATDTTVVTVSVH
                     RFETERGAKDALPYFVSGSNLAEVDKAPSYGEESTTVQGQLEAGSAYVIYIRQNNFIL
                     RISGFSAEGDPAETTQGVAERILAK"
     gene            2443..3165
                     /locus_tag="VFI12_04720"
     CDS             2443..3165
                     /locus_tag="VFI12_04720"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HET7055743.1"
                     /translation="MCGRYVITWQPDEISERFQLRRLPEGMFQTYMNFNAAPTQELPV
                     IVVDESGERVLRPMRWGLRARWSKPGDTKAPAPFNARAESVLEKPMFRNLVKSKRCLV
                     PARGYFEWQNRGDHKQPFLIGLPGDPLIAFAGLYDEFVPQGEEEPVASYTILTTDATE
                     FAGQFHTRMPVILEPEDEADWVDPANTDVMGVLPMAHGYEGEMEAYPVSRAVNNVRNN
                     DPSLLEPIEDPDGEEAEQADLF"
BASE COUNT          652 a         1092 c         1075 g          537 t
ORIGIN      
        1 aatgagggga ggcgacgctg ttgcgccgcc tcccggtgcg tccacgttct gtactggcgc
       61 ggggctgaag ttccgtcatc gacgtgccgc ctcggcctct ggggtaagca cgaccaatcg
      121 catcgagtcg gaaaggtccg agctgcgcag ggtcagcttc tcctccgctt ccggaattgt
      181 tacgccgagc gccttcgtga agggttccag cgtagcaagc tttttatcgc cgatcgcggt
      241 cgcatactgc ataggcagga ctaccttcgg ctcgagatga ccgatcagtt cggccgcctg
      301 ctccgtggtg atcacgtcgc caccgccaac cgggatcatc aggatgtcgc agctgttgag
      361 cacctcgagc ttgtcctcgt tcagcatgtg acccagatcg ccgaggtggc agaatttcat
      421 gccctcgact tcgagcagat aagcggtgtt atagccccgc tccgtgccct tgacgccatc
      481 gtgataggtg cggatgcccg tgatgaagac atcgtgcatc tcgtattcgc ccggaccctc
      541 cacgatctga tacccggact tgacgccggc gaggttggcg tgaccggggt gatcgtggga
      601 gagcaggacg atgtccgcgg tttgcttggc catcgcgtag ccggtgttct tgccgacggg
      661 gtcgatgatg accgtcgcct ccttggcccg aatccggaag cagttatggc cgaaccatcg
      721 tatctctgcc atatccctca gtccggccca gcgggagccg gctccagata aacggaaaac
      781 agcaagccgc ccgttctggt gaggaacgga atgcttgttt ggaggggcaa tacaacgccc
      841 accgcaatct gcggatgtct ggggtgcctc tgtcagtata gcagtcgagg agtcgaggag
      901 tcgaggaggc gagaagcagg gcgcgaaggc acgatgtagg ggtttgcggg ggcaaacccg
      961 ctctacccaa caggcctgtc gctctgcgcc ggcgtagcgc ggtccccccc gtgggccgca
     1021 tcatgcctct tcccacacga tcgatgtcgc cggatcgatt accgggttgg ttcccgtgaa
     1081 caatcggacc gtagatgggt ccgggcggcc cgcgggggac cgcgctacga ttcgcccgaa
     1141 aaccgaaaac cgaaaaccga aaaccgaaaa ccaaagacga aagacccacg cagaaacctc
     1201 acacgcgtgc cggtcgttag tggtgtgacc gaaagtcgat accgggtttt gccgaggtat
     1261 tggcccggct caccgtatac tcatagcgca cgcgccattg tcgacagcat gttccggcgt
     1321 gcacttggcg cgaagccagg aaatcgttcc gcgcaccgca gagagtaggc actcgttcat
     1381 ggagcctcgc ccccaacgtc caacacgcgc gacgggcccg tacatcagca ccggcactcc
     1441 gaatccgccc agcgcgccaa cccccaagtc cggttccggg tatggcactg cgccattcga
     1501 cgcgagcagc gccccgcccg tgccgccgcc cgccatcggg tacgacccgg attactcgcc
     1561 cggccagccg ctgaccccgc tgcgcggtcc gcgcggcgcg aacattgtcg ggccgctgct
     1621 cgccgttgcc gcgctggcaa ttatcgtcgc cgcggtcgct ttcatcgtct cccagttccg
     1681 gggcggcgat accaacaacc cgcagccgac ggcagtcgtc gcggtcaatg ccagcccgac
     1741 ggccgaaacc gatcagcagg ccaacaccgg cgcggtcgac acctccggcg ggaccggaac
     1801 caacgatccg gccgcgcagc cgacttcgga gacggacagc tccgagccga ccaacacgcc
     1861 gcgcgtcaac cggcccaccg agaccggcga cctgagcgga tcgtcggaca gcaacggctc
     1921 cgacgggagc acggccccgg cggatcgcac ccgggcccgg cagtggctcc cgacgacgaa
     1981 gacggtcggt agcggctatc agcagaccga caatgggact cgcgatgccg cgcaggttgc
     2041 caattcgttc ccgaatccgg atgaggcaac caccaatctg accgaattcg gctgggccga
     2101 gaacgcctac cgggagttct cgctgccgga cgggagcgct acggacacga cagtcgtcac
     2161 cgtgagcgtc caccggttcg agacggagcg cggcgccaag gatgcgttgc cgtacttcgt
     2221 gagcggaagc aacctggcgg aggtggacaa ggcgccatcg tatggcgagg agtcgaccac
     2281 cgtgcaaggg cagctcgaag caggcagtgc ctacgtcatc tacatccggc agaacaattt
     2341 cattctcagg atcagcggat tctcggccga gggcgatccc gcggagacga cccagggagt
     2401 tgccgagcgg atcctggcga agtagcgagc gagggcaggc gcatgtgcgg acggtacgtc
     2461 atcacctggc aaccggatga gatctcggag cggttccagc tccgtcgcct gcccgagggc
     2521 atgttccaga cgtatatgaa cttcaacgcg gcgccgacgc aggagttgcc ggttatcgtc
     2581 gtcgacgaat cgggcgagcg cgtcctccgg ccgatgcgct gggggctccg ggcgcgctgg
     2641 tcgaagccgg gcgacacgaa agcaccggcg ccgttcaacg cgcgggccga atcagtgctc
     2701 gagaagccga tgttccggaa cctggtcaag agcaagcgct gcctcgtccc ggcccggggc
     2761 tacttcgaat ggcagaaccg gggcgaccac aagcagccgt tcctgatcgg gctgcccggc
     2821 gatccgctga tcgccttcgc cggcctctac gacgagttcg ttccccaggg cgaggaggag
     2881 ccggtcgcct cgtacacgat cctgacgacc gacgcgaccg agttcgccgg ccaattccac
     2941 acccggatgc ccgttatcct cgaaccggag gacgaggccg actgggtgga cccggcgaat
     3001 acggacgtca tgggcgtcct gccgatggcg cacggatacg agggcgagat ggaggcctat
     3061 ccggtcagcc gcgcggtcaa caatgtccgc aacaacgacc catcgctcct cgagccgatc
     3121 gaagacccgg acggcgaaga ggcagagcaa gccgacctgt tctaactcga gttaagggaa
     3181 cggatgtagg ggtgcattgc aatgtgcccg ccgtgtccga cggaccggtc aactaccggc
     3241 gttcgagccc agggacgccc gggcgtaaac acccgggcct aatcaaataa gctccctgat
     3301 ggtcgttgcc gaacccgttc ggtaatcgga acccggaaac ggagcgcggg gaaacc
//
LOCUS       DASSGX010000269         2704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1358466, whole genome shotgun sequence.
ACCESSION   DASSGX010000269 DASSGX010000000
VERSION     DASSGX010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1358466"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VFI12_04725"
     CDS             complement(<1..282)
                     /locus_tag="VFI12_04725"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HET7055744.1"
                     /translation="MPPPYHVVFMGTPGFAVPSLEALAALSDVELAGVVTQPDRPAGR
                     GRRLTPPPVKFAAERLDVPIMQVTTLRDAAARQRLEAWQADAFIVAAFGL"
     gene            437..>2704
                     /locus_tag="VFI12_04730"
     CDS             437..>2704
                     /locus_tag="VFI12_04730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET7055745.1"
                     /translation="MLLVLILALGFALRLYGLNWDHGTYLHPDERHIVADVMVGRITL
                     SWPPDWSHLLDPARSTINPRPFNPKTGQYSGFAYGTFPVYITELTAQVAQAATRVDWH
                     SYSREVYLGRAWNVLLDTATILLIYLIGRRVLSVTAGLIAALLYAVSPIAIQLAHFFT
                     TESWVMFFTALSLLFAIEAAERGHKRAFFLTGAAFGLALACKSSVFALGGVLALAIIM
                     DAWSRARLYLSIPRQVSALLIRAFLVAVGTVALFFVGEPFGLLDPRAYWDSMSQQASI
                     QSGQWDVPFTREYIGTPRLWYQLGQLVHWGLGPAIGLAGVIGLAFLAWRSVAWLSRGA
                     TVILAWLVLYGAVILLPESKFLRYLLPIVPVFCLAGAALGDRALQWARSGRRIVFAPL
                     VALLIVVPAIATGLSYSAVYAKDQPRLTASVWMFEHLPPGSRTSDEIWDDRLPISLMP
                     GLDSYSRRIDGVSLNLFQDFPTEGDAQQLVTAFNSVSAGQPVARAIAARDYTEAARIL
                     QHAAGAQLASLDADTRVHVAGLLGHAADAMTTSPSLTRATIKAGQTMLQGGEAATGGF
                     ADLEGELAKLPDQTAAAYLYDNLSQADYYVLASNRVMKGIVTAPWRYPVQIAFYDNLF
                     QGKLGFTLVKDFADYPRLGSIAFPDNTADESFINYDHPHVWVFKKTDFPTRAGFSQLF
                     ATSSLQKTYAQREAPDQKSLMLDQPVDQLPVVSDARWSAAWTSHSWVALGFWVVLLIV
                     LQVAARPLVDLVFAGF"
BASE COUNT          394 a          964 c          834 g          512 t
ORIGIN      
        1 cagcccgaac gcggccacga tgaacgcatc ggcctgccac gcttcgaggc gttgacgtgc
       61 cgccgcgtcg cggagcgtgg tgacctgcat gatcggcacg tcgagccgct ccgccgcgaa
      121 cttcactggc ggcggcgtca gacgccgacc gcgaccggcc ggccggtccg gttgcgtcac
      181 cacgccggcg agttccacgt ccgacagcgc ggccagcgcc tccagggatg gcaccgcgaa
      241 cccgggggtg cccatgaaga cgacgtgata cggcggcggc atgctctctc ctccttcgac
      301 cgctcacgga tcatgctagt cttagccgcg gtgaagcggc tggccgcaag ctgattgatg
      361 cacgaggcga ggacgtttga tggaagcgcg cgcggggcaa ccacgggaac gatcggcgcg
      421 agcggcgctc cctatcatgc ttctcgtgct gattctcgca ctcggcttcg cgcttcgtct
      481 ttacggcctg aactgggatc acgggaccta tctccacccg gacgaacggc acattgtcgc
      541 ggatgtcatg gttggccgga tcaccctgag ctggccaccg gactggtcgc acctgctcga
      601 tccggcgaga agtacgatca atccgcgccc gttcaatccg aagaccgggc agtattccgg
      661 cttcgcctac ggcaccttcc cggtctacat cactgagctc accgcccagg tcgcgcaagc
      721 ggcgacccgc gtcgactggc actcatacag ccgtgaggtc tacctggggc gcgcctggaa
      781 cgtgctgctc gacaccgcga cgatcctcct gatctacctt attggccgcc gcgtcctctc
      841 ggtcaccgcc ggcctgatcg cggccttgct ttacgccgtt tcgccaatcg cgatccagct
      901 cgcccatttc tttaccaccg aatcctgggt catgttcttc accgcgctca gcctgctctt
      961 cgcgatcgag gcggccgaaa ggggccacaa acgcgccttc ttcctaaccg gcgccgcctt
     1021 cggcctcgcg ttggcgtgca agagcagcgt cttcgccctc ggcggcgtgc tcgcattggc
     1081 gatcatcatg gacgcctggt cccgcgcgcg cctttatctc tcgatcccgc gacaggtgtc
     1141 ggcgctgctc atccgcgcct ttttggtggc agtgggcacg gtcgccctct tcttcgtcgg
     1201 tgagccgttc gggctgctcg atccgcgcgc gtactgggac tcgatgagcc agcaggcgtc
     1261 gatccagtcc gggcagtggg atgtgccctt cacccgcgag tacatcggca cgccgcgcct
     1321 ctggtatcag ctcggccagc tcgtgcactg ggggctggga ccggccatcg gccttgcggg
     1381 cgtgatcggc ctcgcgttcc tcgcctggcg atcggtcgcg tggctcagcc ggggcgcgac
     1441 cgtcatcctc gcctggctcg tgctctatgg ggctgtcatt ctcctgccgg agtcgaagtt
     1501 cctgcgttac ctcttgccga tcgtgcctgt cttttgtctg gcgggagccg cgctcggcga
     1561 ccgcgcgctg caatgggcgc ggtcgggccg gcgcatcgtc ttcgcgccgc tggtcgcact
     1621 gctcatcgtc gtgccggcga tcgccaccgg tctcagctac agcgccgtct acgccaagga
     1681 tcagccgcgg ctgaccgcgt cggtctggat gttcgagcat ctgcctccag gcagccgcac
     1741 gagcgacgag atctgggacg accggctgcc gatctccctc atgcccgggc tcgactcgta
     1801 ttcccggcgc atcgacggcg tcagtctcaa cttgttccag gattttccca ccgagggcga
     1861 tgcgcagcag cttgtgaccg ccttcaactc ggtgtcggcg gggcagccgg tcgctcgagc
     1921 catcgcggcg cgggactaca cggaagcggc gcgaatcctg cagcacgctg ccggcgccca
     1981 gctcgcttcg ctcgacgctg acacgcgggt ccacgtcgcc ggtctgctcg gccacgccgc
     2041 cgacgcaatg accaccagcc cgtcgctcac ccgggcgacg atcaaggctg gacagacgat
     2101 gctccagggc ggagaggcgg cgaccggcgg cttcgccgac ctggagggag agctcgccaa
     2161 gctaccggac cagaccgccg ccgcctatct ctacgacaat ctctcgcagg ccgactacta
     2221 cgtgctcgcc tccaaccgcg tgatgaaagg catcgtcacc gccccatggc gctacccggt
     2281 ccagatcgcg ttttatgaca atctcttcca gggcaagctc gggttcacgt tggtcaagga
     2341 cttcgcggac tacccgcggc tcggctccat cgccttcccg gacaacaccg ccgacgagtc
     2401 gttcatcaac tacgaccatc cgcacgtctg ggtcttcaag aagaccgact tcccgacccg
     2461 cgccggcttt agccagcttt tcgctacctc atcgctccag aagacctatg cccagcgcga
     2521 ggcgcccgat cagaagtcgt tgatgctcga ccagccggtc gatcagctcc ccgtcgtcag
     2581 cgatgcccgc tggagcgcgg cgtggacgag ccattcctgg gttgcgttgg gtttctgggt
     2641 cgtgctcctg atcgtcctgc aggtcgccgc gcggcctttg gtcgacctcg tcttcgccgg
     2701 gttc
//
LOCUS       DASSGX010000270         4000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_486472, whole genome shotgun sequence.
ACCESSION   DASSGX010000270 DASSGX010000000
VERSION     DASSGX010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486472"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1988)
                     /locus_tag="VFI12_04735"
     CDS             complement(<1..1988)
                     /locus_tag="VFI12_04735"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HET7055746.1"
                     /translation="MPIRRLLATLLLLAGSRAFADVDPVLFQDLHWRLLGPFRGGRVL
                     AVTGIPGQPEHFYFGSVNGGVWETLDAGHTWKPIFDGQPIGSIGALAVAPSDPKVIYV
                     GSGEADMRSDIAQGDGMYKSTDAGRTWTRIGLADSQQIGRILVHPDHPEVVYVAALGH
                     PYGPNAERGVFRSRDGGRTWRKVLGKDDNTGAIDLAFEPGRPEVIYASLWQTRRTPWN
                     VYPPSNGPGSGLYKSTDGGDTWTQLRTGLPADPGRIGVAVAPSEPRRVYAMVDAKEGG
                     LYRSDDAGATWTRTSGDKRIWSRGWYFGGITVEPRNADTVYSCNVNLYRSEDGGRTFV
                     PVRGAPGGDDYHQLWIDPRSPERRILGVDQGATVSINGGATWSSWYNQPTGQFYRVTT
                     DARFPYWVYGAQQDSGAAGVPSRTNTYDGINLTQFREVTAGGENDNIAPDPRDPDVLY
                     GGRVDRLDLRTGLTRSVDPTLGHPDHYRSVWTLPLVFSRRDPRVLYFSNQRLFRTVDG
                     GEHWTVISPDLTREDPGVPANLDPVTAKNSDEPGPRRGVIYAIAPSRLADHDLWVGTD
                     DGLIWRSRDEGGHWTNVTPAELTPWSKVGILDASHFDAETAYAAVDRHRLDDFKPYIY
                     RTHDGGRHWQSIANGIPDGSFVNAVREDAVRPGLLYAGT"
     gene            complement(2081..2965)
                     /locus_tag="VFI12_04740"
     CDS             complement(2081..2965)
                     /locus_tag="VFI12_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055747.1"
                     /translation="MPSMAHEILVDLFKSCPPLAAEILAEVLGVAFPDYTEARIVSAD
                     LSEIQPAEYRADAVVVLLDGKVSVRVIIVEVQLAVDPRKRLSWPAYVTVSRAIHGCPA
                     ALLVVAPEPIVAGWCAESIETGMPGFVLRPFVLHRTAVPVVTDLAEAVRRPELGVLSA
                     MAHGESDQGAMIAAAVLPALQGLDEDRATFYYDLVYNSMNDAARRALEETMKGYEYQS
                     DFAKKYVAQGRSEGRSEGRSEEAARNLLTVLRVRGIAVPDAARERILAQKDPEQLERW
                     LEKAVVASSVTAILDEAN"
     gene            3197..>4000
                     /locus_tag="VFI12_04745"
     CDS             3197..>4000
                     /locus_tag="VFI12_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055748.1"
                     /translation="MRRCIAISATIMFLAQASYARPSGEQSSGQELLQKVKQALKAPG
                     KAWAGTMVSGKAVRANVEHAYSFAFLPDGRFVQSFKGSDSKTLGNDGRRYWQEDSSHV
                     VQELDFGDRDRLMAINLLLSGNWLFAPAPIQLTASEGVVHLVFKETGMAVDIHVDPGT
                     YLPTDASFQTPGGTVLKLSDWRVAGDRRVPMHVEVTTDGDTETYSADAARRIRPGDVH
                     TTMPKQDVSDFSFDAAKPATVEASFAGRTHILVRALVNGQDVGWFLLDSA"
BASE COUNT          663 a         1287 c         1385 g          665 t
ORIGIN      
        1 gttccggcgt agagcagccc cggccggacc gcgtcctcgc gcaccgcgtt cacgaagctg
       61 ccgtccggga tgccgttcgc gatgctctgc cagtgccgtc cgccgtcgtg cgtgcggtag
      121 atgtacggct tgaaatcgtc gaggcggtgg cggtccacgg ccgcgtacgc ggtctccgcg
      181 tcgaaatggg aggcgtcgag gatgcccacc ttggaccacg gggtcagctc cgcaggcgtg
      241 acgttggtcc agtgcccgcc ttcgtcccgc gagcgccaga tcaggccgtc gtcggtgccc
      301 acccagagat cgtgatcggc cagccgcgac ggcgcgatgg catagatcac gccccggcgc
      361 ggtcccggct cgtcgctgtt cttcgcggtc accggatcga ggttcgccgg cacgcccggg
      421 tcctcgcggg tgagatcggg actgatcacc gtccagtgct cgccgccgtc cacggtgcgg
      481 aacagccgct ggttggaaaa atagagcacg cgcggatcgc ggcgcgaaaa cacgagcggc
      541 agcgtccaga ccgagcggta gtgatcggga tggccgagcg tcgggtcgac cgaccgtgtc
      601 agcccggtgc gcaggtcgag gcggtccacc cggccgccgt agagcacgtc cggatcccgc
      661 ggatcgggcg cgatgttgtc attctctccg cccgcggtga cctcgcggaa ctgggtgagg
      721 ttgataccgt cgtacgtgtt ggtgcggctg gggactccgg cggctccgga atcctgttgc
      781 gcgccataga cccagtaggg aaaccgggcg tcggtggtga cgcgataaaa ctgaccggtc
      841 ggctggttgt accacgagct ccaggtcgcg ccgccgttga tcgataccgt cgcgccctga
      901 tcgaccccga ggatgcgccg ctccgggctc cgcggatcga tccagagctg atggtaatcg
      961 tcgccgcccg gcgcgccgcg gaccggcacg aacgtccggc cgccgtcctc cgagcgatac
     1021 aggttgacgt tgcaggagta gaccgtgtcg gcgttgcgcg gctcgaccgt gatgccgccg
     1081 aagtaccagc cgcggctcca gatgcgcttg tcgccgctcg tccgcgtcca ggtggccccg
     1141 gcgtcgtccg accggtagag cccgccctcc ttggcgtcca ccatggcata cacccgccgc
     1201 ggctcgctcg gcgccaccgc gacgccgatc ctcccggggt ccgccggcag gccggtccgg
     1261 agctgggtcc aggtgtcgcc gccgtcggtc gatttgtaga ggccgctgcc cgggccgttc
     1321 gacggcgggt agacgttcca gggcgtgcgc cgcgtctgcc acagggaggc gtagatcacc
     1381 tccggccggc cgggctcgaa cgcgagatcg atggcgccgg tgttgtcgtc cttgccgagc
     1441 accttccgcc aggtccggcc gccgtcccgc gagcggaaca cgccgcgctc ggcgttcggc
     1501 ccataggggt gaccgagggc cgcgacatag acgacctcgg gatggtccgg gtgaaccagg
     1561 atccggccga tctgctgcga gtcggcgagg ccgatgcggg tccaggtgcg gccggcgtcg
     1621 gtggacttgt acatgccgtc gccctgggcg atgtcggacc gcatgtcggc ctcgcccgag
     1681 cccacataga tcaccttcgg gtccgacggc gccacggcga gcgcgccgat ggagccgatg
     1741 ggctgtccgt cgaagatcgg cttccacgtg tggccggcgt cgagcgtctc ccagacgccg
     1801 ccgttgacgc tgccgaagta gaagtgctcc ggctggcccg ggattccggt caccgcaagg
     1861 acgcggccgc cgcggaacgg gccgagcagg cgccaatgga ggtcctggaa gagaaccggg
     1921 tccacgtccg cgaacgcgcg ggagccggcg agcaggagga gggtagcgag caggcggcgg
     1981 atcggcatgg cagggccgat gatatccgct ttggccggcg ctcaggtcgg gtggtggcgt
     2041 cctctcccgc gcagcggggg aggatcgagg agggggctcc tcagttcgct tcgtcgagga
     2101 tcgccgtgac cgaagaggcc acgacagcct tctccagcca gcgttccaac tgctccggat
     2161 ccttttgggc cagaatacgc tcgcgggcgg cgtctggcac cgcgatgccg cgaacccgga
     2221 ggacagtcag cagattgcgc gccgcctcct cggaacgtcc ctcggaacgt ccctcggagc
     2281 gtccctgtgc gacatacttc ttcgcgaaat cgctctggta ttcgtaaccc ttcatcgttt
     2341 cctccaacgc acggcgggcc gcgtcgttca tggaattgta cacaaggtcg tagtagaagg
     2401 tggcccgatc ttcgtcaagt ccctggagcg cgggcagcac cgcggcggcg atcatcgctc
     2461 cctggtcgga ctctccgtgc gccatcgcgg agagcacgcc aagctcaggc cggcggaccg
     2521 cctccgcgag gtccgtcaca accggaacgg cggtgcggtg aagtacgaag gggcgcagga
     2581 cgaagcccgg catgccggtc tcgatcgatt ccgcgcacca gccggcgacg atcggctcgg
     2641 gggcgacgac gaggagagcc gccgggcagc cgtgaatggc acgggagacc gtgacgtagg
     2701 cgggccagct cagacgcttt ctggggtcca ccgccagttg gacctcgaca atgatcaccc
     2761 ggacggagac cttgccgtcc agcaggacga cgacggcgtc ggcgcggtat tcggcgggtt
     2821 ggatctcgct caagtcggcc gaaacgatgc gggcttcggt gtagtcaggg aacgcgacgc
     2881 cgagcacctc ggccaggatt tcagcggcga gaggggggca gctcttgaac agatccacaa
     2941 ggatctcgtg ggccatggat ggcatggcgg cgatgctcgc atacttgctg gaaggggtca
     3001 aacatgcccg gtcccgggaa gatttcccgg acagggttcc caggaacgat gccggcacgc
     3061 gccgccggcg agcttctgag cggtcggccg tcaggcaact ggaggctcaa gtgaggttta
     3121 tgattcagac gaagcccagg tgccgttcgt cggcaaacgg gaggctccct tcggactcct
     3181 tgtggtagga ggttctatgc ggcggtgcat cgcgatcagc gcgacgatca tgtttctcgc
     3241 tcaggcgagc tacgcgcggc cctccggcga acagagctcc ggtcaagagt tactccaaaa
     3301 ggtgaagcag gctttgaaag cccccggcaa ggcttgggcg gggacgatgg tttcggggaa
     3361 agccgttcga gccaacgtcg aacatgccta ttccttcgcc tttctgccgg acggacggtt
     3421 tgtgcaatcg ttcaagggat cggattctaa aactctcgga aacgatggac ggcggtactg
     3481 gcaagaggac tcttcgcatg tcgtgcaaga gctcgatttt ggagaccggg acaggctaat
     3541 ggcgatcaac ctcctgctct ccggcaattg gctcttcgcg cccgctccca tccaacttac
     3601 agcaagcgaa ggggttgtcc accttgtctt caaggaaacg ggaatggccg tcgatattca
     3661 cgtcgatccc ggcacttatc ttccaacgga cgcctctttt caaaccccgg gtggaacggt
     3721 gctgaaactg agcgattggc gggttgcagg ggaccgaaga gtcccgatgc acgtcgaggt
     3781 tacgaccgac ggcgataccg agacctacag cgcagatgca gcccggcgga ttcgccccgg
     3841 cgacgttcat accacgatgc cgaaacagga cgtctccgac ttcagcttcg atgcggcaaa
     3901 gcccgccacc gtcgaggcga gtttcgcagg gaggacccac atcctggttc gcgctctggt
     3961 caacggccag gatgtcgggt ggttcctcct ggacagcgcc
//
LOCUS       DASSGX010000271         2316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1467955, whole genome shotgun sequence.
ACCESSION   DASSGX010000271 DASSGX010000000
VERSION     DASSGX010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1467955"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VFI12_04750"
     CDS             complement(<1..394)
                     /locus_tag="VFI12_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055749.1"
                     /translation="MRYRISAFWLVSVLVTLAFTLGPVGIVAAQEATPAATPGSEVTV
                     VASGLTNPRGFTWGDDGTLYLALAGTGGTEQLVVEATPMPFFPGATASIVTVANGCTT
                     TIADGLPSIMLAEPGWVFGVTDVATLNGQ"
     gene            complement(557..1108)
                     /locus_tag="VFI12_04755"
     CDS             complement(557..1108)
                     /locus_tag="VFI12_04755"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HET7055750.1"
                     /translation="MTKDQTIRYVALLRGINVGGHRMIKKDELTAIFAGCGFTDVTPV
                     LASGNVVFSSDLVDEAALKERIETALNDALGYSVDVMVRTIAYLQDLIALDPFGEVGP
                     GDGHNYVTFLTHAPEQAPDLPEDLPDQYALALGLHDREFFAVSRKRPDGRYGDFAAYM
                     RQHFGKQPVTTRNWKTVVKLAAM"
     gene            1305..1844
                     /locus_tag="VFI12_04760"
     CDS             1305..1844
                     /locus_tag="VFI12_04760"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HET7055751.1"
                     /translation="MSQVVDPAHVDGEEIDLDVVREILSHFTYHDFQEAQEQLLGALM
                     EVNHRFGWVSLAAAKVVADYFGTSDNRVYALLTFYADFRTQPRGKHFMLLCHGMSCYV
                     MGSRKLIDELEQKYEVVDGGTTADGELTIQVVNGCLGVCDRAPVVELDHEFHTDLTVE
                     KFNDVLRKAIANGGGWRHH"
     gene            1846..>2316
                     /locus_tag="VFI12_04765"
     CDS             1846..>2316
                     /locus_tag="VFI12_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055752.1"
                     /translation="MQMPGDSTLPRLTSRGEFEQYAATATDKWNAMWSSMNTVVTVLV
                     GSSSMAKGSEKVLDAAAEYALMKSGIVVRKSGVDGADWMEVQVRIKRPGAPEVHYADV
                     APTDIVDLIEGRLEEKALGVDGDQSYNGIPPMKDHPFFKYQQRLILRDVGVIDPE"
BASE COUNT          427 a          787 c          673 g          429 t
ORIGIN      
        1 cctggccatt cagcgtggca acgtccgtga cgccgaatac ccagccgggt tctgcgagca
       61 tgatcgacgg caacccgtca gcgatcgtgg tagtgcagcc gttcgcgacc gtcacgatgc
      121 tcgcagtcgc gccgggaaag aacggcatcg gcgtggcttc gaccacgagt tgctccgtgc
      181 caccggtgcc agccagggcg agatagagcg tgccgtcgtc gccccatgtg aagccgcgcg
      241 gattggtcaa cccgctcgcg acgaccgtga cttccgagcc cggggtggcg gccggcgtag
      301 cttcctgcgc cgcgactatc cccaccggac ccagcgtaaa tgccagggta accaggaccg
      361 aaacgagcca gaacgcggaa atccggtaac gcatgacttc ctcccctgtg aagcaatcga
      421 agccgccgga ctcacatctg tctgtagcgt gtgtaactct tatcctatca cgaccttttg
      481 atacgtcaag agtacgaatg gcgggatgag aagtgggcgc atccctcgcc gcgccccagc
      541 tgctcgcctt cgcgcctcac atcgcggcca gcttcaccac cgtcttccag ttccgcgtcg
      601 tgaccggttg cttgccgaag tgctgccgca tgtacgccgc gaaatcgccg tagcgcccat
      661 ccggacgctt ccgggaaacc gcgaagaact cgcgatcatg cagcccgagc gccagcgcat
      721 attgatccgg caaatcctcg ggcagatccg gcgcctgctc cggcgcgtgc gtcaggaagg
      781 tcacgtagtt gtgtccgtcg cccggcccga cctcgccgaa cgggtccagc gcgatcaggt
      841 cctgcaggta cgcgatcgtc cgcaccatga catcgaccga atagccgagg gcatcgttga
      901 gcgcggtctc gatccgctcc ttcagcgccg cctcatcgac caggtcgctg ctgaacacga
      961 cattcccgct tgccagcacc ggcgtgacat cggtgaaccc gcatcccgca aagatcgccg
     1021 tcagctcatc cttcttgatc atccgatgcc cgcccacatt gatcccgcgc agcaacgcca
     1081 cgtaccggat ggtctggtct ttagtcattg gtctctcgtc tttcgtcttt ggtggttttc
     1141 cgttttccgt tttccgtttt ccgtgcttgc taccagctac tggctaccgg caactgtccc
     1201 atgtcgttcg cacgactcac gtctcacgac tcacgactcc tttatactca cccttggcgg
     1261 tccgcgtacc ggccgccgtc atgccgttca gaaggagcta ttgcgtgtcc caggtggttg
     1321 accccgcaca cgtcgacggt gaagagatcg atctcgatgt cgtgcgcgaa atcctctccc
     1381 acttcacgta ccacgatttc caggaagccc aggagcaact cctcggtgcc ctgatggagg
     1441 tcaaccatcg gttcgggtgg gtctcgctcg cagccgccaa ggttgtcgcg gattacttcg
     1501 gcacgagcga caaccgcgtc tacgcgctcc tcaccttcta cgccgacttc cggacccagc
     1561 cgcgcggcaa gcacttcatg ctcctttgcc acggcatgtc ctgctatgtc atgggctccc
     1621 gcaagctgat cgacgagctg gagcagaagt atgaggtcgt cgatggcggc accacggccg
     1681 acggcgagct gacgatccag gtcgtgaacg gctgcctcgg cgtctgcgac agggcgccgg
     1741 tcgtcgagct cgaccacgaa ttccacaccg acctgaccgt cgaaaagttc aacgacgtcc
     1801 tgcggaaggc aatcgccaac ggcggcggat ggagacacca ctgatatgca gatgcccggt
     1861 gattcgaccc tgccgcgcct gacctctcgc ggcgaattcg agcaatacgc ggccacggcg
     1921 accgacaagt ggaacgccat gtggtcgtcc atgaacacgg tcgtgaccgt cctcgtcggc
     1981 agttcatcga tggcgaaggg ctccgagaaa gtgctcgacg ccgccgccga gtacgccctc
     2041 atgaaatccg gcatcgtcgt ccgcaagtcg ggcgtcgacg gcgcggactg gatggaggtc
     2101 caggtccgga tcaagcgacc gggcgcgcct gaggtccact acgccgacgt cgccccaacc
     2161 gacatagtcg acctgatcga gggccggctc gaggagaagg cgctcggcgt cgacggcgac
     2221 cagtcgtaca acggcattcc gccgatgaag gaccacccct tcttcaagta ccagcagcgt
     2281 ctcatcctcc gggacgtcgg cgtgatcgac ccggag
//
LOCUS       DASSGX010000272         2865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1358700, whole genome shotgun sequence.
ACCESSION   DASSGX010000272 DASSGX010000000
VERSION     DASSGX010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1358700"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VFI12_04770"
     CDS             complement(<1..309)
                     /locus_tag="VFI12_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055753.1"
                     /translation="MAEAPIGAVGIIANPASGKDIRRLVAHASTFDNNEKINIVRRAL
                     LALDSLGIPEVWYLPDSYAIVERAAGSADLALRLVPLPMEVIGNASDSYEAAARMADA
                     G"
     gene            551..2134
                     /gene="aceB"
                     /locus_tag="VFI12_04775"
     CDS             551..2134
                     /gene="aceB"
                     /locus_tag="VFI12_04775"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HET7055754.1"
                     /translation="MANGVMLTADIPEHAAHVFTDEALDFVADLHRTFNARRLELLDL
                     RAQRVARIPTSFGLDFLPETKSVRDGDWRVAPATSGLIDRRVEITGPTDRKMMINALN
                     SGAKVFMADLEDANSPTWQNVVEGQVNLYDAVRHQIRFEQEDGKVYELEPVIATLVVR
                     PRGWHLVERHVEVDGEPASASLVDFGLTFFNNGAEQGLRGLGPYFYLPKLESHLEARL
                     WNDVFVWAQERIGMPQGSIRATVLIETIFAAFEMDEILYELREHAAGLNAGRWDYIFS
                     LIKTFSSSPNHILPDRAQVTMAVPFMKAYSDLLVKTCHQRGAHAIGGMAAFIPTRRDP
                     EANELAFAKVREDKQREANAGFDGTWVAHPGLVPVAEAEFNAVLGDKPNQTDRQRTDV
                     EPTAAALLTTDIAGGQVTEAGLRTNISVAIQYLDNWLRGNGAAAINNLMEDAATAEIS
                     RAQIWQWIRHRVRTEDGEPITVERYRALRDEELAKLNNPAGNRYADAATILNLLIESE
                     AFIPFLTIPAYDFLTAEDA"
     gene            2131..>2865
                     /locus_tag="VFI12_04780"
     CDS             2131..>2865
                     /locus_tag="VFI12_04780"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HET7055755.1"
                     /translation="MITTGTPQLLVSGFNSPEGPAFDRDGNLFFVNWLTSSVNRMDSD
                     GNVVEFYNTGGIPAGLAFDRDGMLWVADEGDDIHGLLRIAPDGHAEIVVNEYEGAPLN
                     GANDLVIDRNGVIYFSDPWRSNAENPVGGFYRYFPDGRLEQIDSGLAFPNGVALNADE
                     SAVYLAETYRTRILRYEIRPDGSLSPRQVWGDTGKPAGPDGMAFDAEGNLYCAHHGGG
                     RVDIFNPDGERIGEIPIPGANVTNCAF"
BASE COUNT          530 a          987 c          894 g          454 t
ORIGIN      
        1 cccggcatcg gccatccggg ccgccgcctc atacgaatcg ctcgcgttcc cgatcacctc
       61 catcggcaat gggaccagcc tcagcgccag gtccgcgctg ccggctgctc gctcgacgat
      121 cgcgtagctg tccggcaggt accagacctc ggggatgccg agcgaatcga gcgcgagcag
      181 ggcgcgacgg acgatattga tcttctcgtt gttgtcgaag gtcgacgcgt gggcgacgag
      241 ccggcgaatg tccttgcccg aggccggatt cgcgatgatc ccgaccgccc cgattggcgc
      301 ctccgccatg taaatgccct cccccgaacc gtttgcgacc gccagccacg gcctgtgtga
      361 gtgtagccgg gcaacggatc cgccgtgttg ccggcccgct ccctgccgcg ccggaagccg
      421 gtaccgtttc gccggacacc gcaatcgctc ccgggtgtgc ggcgcggatt cccccgcttt
      481 cgggctacga cccccccaaa cgtgcgaaga tggtgtcaga gcgaacaggc aatcaccagg
      541 ggaggtgacc atggccaatg gggtaatgct gactgccgat attccggaac acgctgccca
      601 cgtctttaca gacgaagcgc tggatttcgt cgccgatctg caccggacgt tcaacgcgcg
      661 ccgcctggag ctgctcgacc tgcgagccca acgcgtcgcc cggatcccga ccagcttcgg
      721 gctcgatttc ctgcccgaaa ccaaatcggt ccgcgatggc gattggcggg tcgccccggc
      781 gacgtccggc ctgatcgacc ggcgcgtcga aatcaccggc ccaaccgacc gcaagatgat
      841 gatcaacgcc ctcaactccg gcgcgaaggt cttcatggcc gacctcgagg acgccaactc
      901 gccgacctgg cagaacgtgg tcgaagggca ggtcaacctt tacgacgccg tccgccacca
      961 gattcgtttc gagcaagagg atggcaaggt ctacgagctg gagccggtaa tcgcgacgct
     1021 cgtcgttcgt ccgcgcggct ggcacctcgt cgagcggcat gtcgaggtcg acggcgagcc
     1081 ggcctcggcc tcgctggtcg atttcggact gaccttcttc aacaacggcg ccgagcaggg
     1141 actgcgcggg ctcgggccgt atttctacct gcccaagctg gagagccacc tcgaagcgcg
     1201 actctggaac gacgtcttcg tctgggcgca agagcggatc ggcatgccgc agggctcgat
     1261 ccgtgcgact gtcctgatcg agacgatctt cgccgccttc gagatggacg agatcctcta
     1321 cgagttgcgg gaacatgccg ccgggctcaa cgccggtcgc tgggactaca tcttcagcct
     1381 gatcaagacc ttcagcagca gtccaaacca catcttgccg gacagggcgc aggtcacgat
     1441 ggctgtgccc ttcatgaagg cgtacagcga cctcctggtg aagacatgcc accagcgcgg
     1501 cgcccacgcg atcggcggga tggccgcctt catcccgact cgccgagatc ccgaagccaa
     1561 tgagctggcc ttcgccaagg tgcgggagga caagcagcgc gaggccaacg ccggattcga
     1621 cggcacgtgg gtcgcgcatc cgggcctggt cccggtggcc gaggccgagt tcaacgccgt
     1681 cctcggcgac aagccgaacc agaccgatcg ccagcgtact gacgtagagc cgacagccgc
     1741 cgccctgctc actacggaca tcgccggcgg gcaggtgacg gaagccgggc tccgcacgaa
     1801 catcagcgtc gcgatccagt atctcgacaa ctggctgcgc ggcaacggcg cggccgcgat
     1861 caacaacctg atggaagacg ccgctaccgc cgagatctca cgcgcccaga tctggcaatg
     1921 gatccggcac cgcgtccgca ccgaggatgg cgagccgatc accgtcgagc ggtaccgcgc
     1981 ccttcgagac gaagagctag ccaagctcaa caacccggcc ggaaaccggt acgcagacgc
     2041 cgccacgatc ctgaacctgc tgatcgagag cgaggcgttc atccccttcc tgaccatccc
     2101 ggcctacgac ttcctgacgg cggaggacgc atgatcacga ccgggacgcc gcaactgctg
     2161 gtcagcggct tcaactcgcc ggaagggccg gccttcgatc gtgacggcaa cctcttcttc
     2221 gtcaactggc tgaccagctc ggtcaaccgg atggattcag acgggaacgt tgtcgagttc
     2281 tacaacaccg gcggcatccc ggctggactc gccttcgacc gcgacggaat gctctgggtg
     2341 gccgatgagg gcgacgacat ccacggtttg cttcggattg cgccggacgg ccacgcggag
     2401 atcgtcgtca acgagtacga gggagcgcca ctgaacggcg caaacgacct cgtgatcgac
     2461 cggaacggtg tcatctactt ctccgacccc tggcggtcga atgcggagaa cccggtcggc
     2521 ggcttctacc gctacttccc ggacggccgg ctcgagcaga tcgattccgg cctcgccttc
     2581 ccgaacggtg tcgcgctcaa tgcggacgag tcagccgtct acctcgccga gacctaccga
     2641 acgcgaatcc tgcgctacga gattcggcct gatggctcgc tgagtccgcg tcaggtgtgg
     2701 ggagataccg gaaagccggc cgggcccgac ggcatggcgt tcgacgccga gggcaacctc
     2761 tactgcgcgc accacggcgg cggccgggtc gacatcttca acccggacgg cgagcgaatc
     2821 ggcgaaatcc cgatccccgg tgcgaacgtc accaactgcg ccttc
//
LOCUS       DASSGX010000273         3052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1468089, whole genome shotgun sequence.
ACCESSION   DASSGX010000273 DASSGX010000000
VERSION     DASSGX010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1468089"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..775)
                     /locus_tag="VFI12_04785"
     CDS             complement(<1..775)
                     /locus_tag="VFI12_04785"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HET7055756.1"
                     /translation="MKAAGAATAMTIGGRTIASAQQVSGLPAGMALVASPRLPLYGIG
                     SADVANVLSGAVPDWRELGSAVSLKVEPIALAGGTPTAGTPATTVNDYEALAAELDKR
                     PGGVALVPADQVDFRASVIAVDGYDPVRHEDGAIRVGVVGDIVPGRNVNNKMVAFGDY
                     THPFRKVAAELSSYDVMIANLEGNLSNTLQPPEDAHTFTFVSSPQMVDGFKLAGIDAV
                     TLANNHSMWNSEGWGAQGLLDTIDALQGGGIPHFGAGNDL"
     gene            complement(1021..1983)
                     /locus_tag="VFI12_04790"
     CDS             complement(1021..1983)
                     /locus_tag="VFI12_04790"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HET7055757.1"
                     /translation="MRVPQRRYGEVVALKWAGVLLILLAALTAFALIVRDIRSDPEST
                     PLPAPQLVIPAASGGTGSDPAAAIAPNPAFQGKVICLDAAHGGVDRGFRRTGDSTAPA
                     MDEALYTAAYARELAGQLTAMGFTVVQTRDGDTVRNTQFQDVNRDGQTRESGVTDADA
                     QHSAQLDEMQARIDYCNDEQADLLISLHFDGSTNPGVSGFSTWYAEGRTDSAESQRLA
                     ELIDQQLGRGLTAAGYSVVDDGALPESGAVTRGNQMLYDSLFMITGARDGLKDPSRMP
                     GVVAELLTISNASDAQLLASEAGREAIVSALAQAISDYFTGTTP"
     gene            complement(2464..2937)
                     /locus_tag="VFI12_04795"
     CDS             complement(2464..2937)
                     /locus_tag="VFI12_04795"
                     /inference="COORDINATES: protein motif:HMM:NF024861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HET7055758.1"
                     /translation="MSRVDSSTLVFFDASCLIAAAVSSTGGSAFVWRLCERGLLSAAV
                     SLAVLAEAESNLSAKFEPECLQRHRHQLRAGAPRVAPIPRLDVQPRLFPGINPKDEHV
                     VAAAHAIRADILLTLDQPLAAEIERSGLGILARSPGECIRLDLPAHSLFASLREE"
BASE COUNT          575 a          947 c         1021 g          509 t
ORIGIN      
        1 agaggtcgtt tccggcgcca aaatggggaa tgccgccacc ctgcaacgca tcgatcgtgt
       61 cgagcagtcc ctgagcgccc cagccttcgc tattccacat cgaatggttg ttcgccagcg
      121 tcaccgcgtc gatcccggcc agcttgaagc cgtcgaccat ctgcgggctg gaaacgaagg
      181 tgaaggtgtg cgcgtcttcg ggcggttgca gcgtgttcga gagattgcct tcgaggttgg
      241 cgatcatgac atcgtacgag ctcagctcgg cggcgacctt gcggaaggga tgggtgtagt
      301 cgccaaaagc gaccatcttg ttgttgacat tgcggccggg cacgatatcg cccaccacgc
      361 ccacgcggat cgcgccgtct tcgtgtcgca ccggatcgta accatcgacg gcaatgacgc
      421 tggcacggaa atcgacctgg tcggccggca ccagcgcgac cccgccggga cgtttgtcca
      481 gctccgccgc cagcgcctcg tagtcgttca ccgtcgttgc cggtgtgccg gcggtgggcg
      541 tgccgccagc aagcgcgatc ggctcgactt tcagcgaaac cgccgaaccg agctcccgcc
      601 aatcgggaac cgcaccggac agcacgttgg cgacgtcggc gctgccgatg ccatacaggg
      661 gcaagcgggg cgaggccacc agggccatac cggcggggag tccactgacc tgctgggcgg
      721 aagcgatcgt gcgccctcca atcgtcatcg cggtcgccgc gccggcggcc ttcaagacgg
      781 ttcgacgcga aacactccga tcaggaaacg atgacggcac gtggactcct ctcggctcgg
      841 cgggacatcc gccgaattcc ggcaaatcgt accccgagcg cgatcgggaa tcatccctat
      901 ggcggatttg catgtagcaa cttggtcaag agtcaaggat caagggtcag gtgaaacgtc
      961 tactcgtgcc gctctggact cttgaccctt gacccgttct ggcgagttcg cgatcccgcc
     1021 tcagggagtc gttccggtga aataatcgct gatcgcctgc gccagcgcgg agacgatcgc
     1081 ctcgcggccg gcttcgctgg ccagcagctg cgcgtcgctg gcattggaga tggtcagcag
     1141 ctcggcgacc accccgggca tccggctggg gtccttcaac ccatcccgtg cgccggtgat
     1201 catgaagagc gagtcgtaga gcatctggtt gccacgggtg accgcgcccg attccggcag
     1261 ggcgccgtca tcgaccacag aatacccggc ggcggtcagc cctcgaccga gctgctggtc
     1321 gatcagctca gccagccgtt gcgattcagc gctgtcggtt cgaccctcgg cataccaggt
     1381 ggaaaatccg gacacgcccg ggttggtcga gccgtcgaaa tgcaacgaaa tcaacaaatc
     1441 ggcctgctca tcattgcaat agtcgatgcg cgcctgcatc tcgtcgagct gcgcgctgtg
     1501 ttgggcatcg gcgtcggtga cgccgctctc ccgagtctgg ccgtcgcggt ttacatcctg
     1561 gaattgggta ttgcgcacgg tgtcgccgtc acgcgtctgc accacggtga agcccatggc
     1621 cgtgagctga ccagccagct cgcgggcata ggcggcggtg tagagcgcct cgtccatggc
     1681 cggggcggtg gaatcgccgg tgcggcggaa tccgcgatcg accccaccgt gcgccgcgtc
     1741 gaggcagatc acttttccct gaaaggccgg gttgggcgcg atcgcggcgg ccgggtcgct
     1801 gccggttccg ccggacgcgg cgggaatgac gagctggggc gccggtaagg gtgtggattc
     1861 cggatcggag cgaatatccc gcacgatcaa tgcgaaggcc gtcagcgccg ccagcaggat
     1921 cagcaacacc ccggcccatt tgagcgccac cacttcgcca tagcggcgct gcggcacccg
     1981 cacatatggg ggaaccgcgg gagcggccgg ccgcagcggg acgcgttcgg acggataggg
     2041 cagggccgcg gcgctcgtcg aaacggcccg aggcgagggg ccacctggcc ggccgggtgg
     2101 cacacgacga ctcggagccg gtcgcggctg ctcgcggcgt ggaggcagtt gcgggggagg
     2161 tccaagccag ggaagctcgt cctcgaacca atcttcggac atcagcctca gcgggcgagc
     2221 cggatcgttg caggcgatcc cagctcgttc ggtcgacgac gaatgctatg gagtgacgga
     2281 tagcgaagat ggcaatgcgg cactccagcc agtgcgcggc cagcatagga gaacgttcag
     2341 gcgaaatgca agcgagtccg gagttcggag aacgctcatg ccctcggttg ccccgggccc
     2401 aggacgagac cggtcgggcg acacctcacc gaggaaccaa ctcgaccatg cgagccgatg
     2461 cggctattcc tcgcggagtg aggcaaacaa ggaatgcgcg gggaggtcca gccgaataca
     2521 ttctcctggc gaccgagcga gaataccgag gcctgatcgt tcgatttctg cggccagtgg
     2581 ttgatcgagc gtcaagagga tgtctgcgcg aatcgcatga gcagcggcaa cgacatgctc
     2641 gtctttcggg ttgatcccag gaaacaaacg tggctggacg tcgagtcgag gtatcggtgc
     2701 aacgcggggc gcaccggctc gaagttggtg gcggtgacgc tgcaagcatt ccggctcgaa
     2761 tttggcggaa aggttggatt ctgcctccgc cagcaccgca agcgaaacgg ctgcgctcag
     2821 cagtccacgt tcgcatagcc tccagacgaa tgccgatccc ccggttgatg aaacggcggc
     2881 ggcgatcaga caagatgcat cgaaaaagac aagcgtcgag gagtcaacgc gactcatggg
     2941 cgccggaaga tccgtccacg aaggggaacg atccaatcac agcctgggtc tctgcatcga
     3001 gtgcatcttg atcgaggaat gtttgcagtt gccggtcgga ataggtgcgc ag
//
LOCUS       DASSGX010000274         3336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1358847, whole genome shotgun sequence.
ACCESSION   DASSGX010000274 DASSGX010000000
VERSION     DASSGX010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1358847"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="VFI12_04800"
     CDS             complement(<1..813)
                     /locus_tag="VFI12_04800"
                     /inference="COORDINATES: protein motif:HMM:NF020028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055759.1"
                     /translation="MTDMTPPRWDLTGIFPSPISGEYIAAVVNLRANIEQMQQLFDEY
                     GIGLDSGAHPDTDAGKIISTWNEAGDLADLLDNYLYCWTSTDSNDAAAASAYGEFDMI
                     TSPMKALRSRLTTWMGTLRSDALTDPVAKDHRYQVEQARIGAQHVMAPELEDLAGALQ
                     ATGGQAWVRARDESNAGLTASITIDGETQTKSLTELEALLSSPDREKRRIAAEAMDTA
                     HEKAAPAFAASLNAIKGEALTLAKRRQWHSPLDWSLYVSAIDQDILEAMFAAA"
     gene            complement(879..>3336)
                     /locus_tag="VFI12_04805"
     CDS             complement(879..>3336)
                     /locus_tag="VFI12_04805"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HET7055760.1"
                     /translation="NNCEGFHANSSNSICGFVDQDPVFEYGHGSCNSLSGGAFIPNGV
                     WPSTYDGTYFFSDYTCGTIWTLTNSGGTWSRTTFASGLGAVTELEFGPYGSNVALYYA
                     DYGSSQIRRISYNAANNQAPVADIKAAPTFGAVPLTVGFEGRGSSDPDADPLTYRWDF
                     GDGTSSTRSTFSKTYKTAKKFRVTLTVTDGKGGTDTDAVTINAGNTRPSAEITSPSAS
                     SQFSVSQMITLTGLGTDTEDGALSSSRMTWEVLLHHDTHTHPWFGPVKGNNLTFQAPA
                     PEDLKAAGTSYLEIKLTVRDSGGLGKLVTMNLMPNTVNVTVGSNVPGMLLWIEGSPYR
                     APATVLTWANNPLTVVAPDQIGPDDAPYIYKSWSDGGRRSHEYVAPASDSTVTATFNL
                     LPGNAFAPHADARVSEANPTRNEGLGTGLSVKGGTSSDYETLIRFKVSGVGDRVYRAR
                     LYLYAYDPTVDGPSIYTTSSSWRETGLTWNTRPGPTSSQFDNYGPIADNSWIAYDVTS
                     AISGDGYVTFLLRGTSSDAVSWYSRQAGSRQPRLVIWSSGVTTAEVPPEPTTTPEPSP
                     EASPFAQPTESASPEPTEVIETPTPVASPETALPITDDFEGNLSGWSGEGALLAPGTG
                     RDDTQAAQMRSSGSDVVSGTPSYLQRALPAGETNIHVALDVMPRTLDSGVTRFVTITG
                     ADNAAIAALYLGPDGLMGIRFGEGDSLIPVGSIHTSNWSRIEVAFAAESGSATVQIWV
                     NGQYAGSATDVAAPTELQTVVLGGWATDRTFDLLIDNVAIDRGCISGCPAAPAPVETN
                     TPVPAPGDVIAPDPGTPVSG"
BASE COUNT          543 a         1027 c         1137 g          629 t
ORIGIN      
        1 cgctgccgcg aacatggcct cgaggatgtc ctggtcgatc gcgctgacat agagcgacca
       61 gtcgagcgga gaatgccact ggcgccgctt ggcgagggtc agcgcctcac ctttgatcgc
      121 gttcagggag gcggcaaagg ccggcgccgc cttctcgtgg gccgtgtcca tcgcctcggc
      181 ggcaatgcgc cgcttttcgc gatccggcga agagaggagc gcctcgagtt cggtcagtga
      241 cttcgtttgc gtttcgccgt cgatcgtgat cgatgcggtc aggccggcat tcgactcatc
      301 ccgggcgcgg acccatgctt gcccgccggt agcctgcagc gcgccggcca ggtcctcgag
      361 ctccggggcc ataacatgct gcgcgccgat ccgcgcctgc tcgacctgat accggtgatc
      421 cttcgcgacc gggtccgtaa gtgcgtcaga cctgagcgtg cccatccagg tcgtcaggcg
      481 cgagcggagc gctttcatcg gcgacgttat catgtcgaac tcgccgtagg ccgaggccgc
      541 ggcggcatcg ttcgaatcag tcgaagtcca gcagtagagg tagttgtcca gcaggtcagc
      601 gagatccccg gcctcgttcc aggtcgaaat gatcttgccg gcgtctgtat ccggatgtgc
      661 gccggaatcg agcccgatgc cgtactcatc gaagagctgc tgcatctgct caatgttcgc
      721 gcgcaggttc acgacggcag caatgtattc gccgctgatc gggctcggga agatccccgt
      781 caggtcccag cgcggcgggg tcatgtcagt catgcgtgat acgtcctcgt gtcagtgcgc
      841 ggcgcctctt cgtgccgcgg cgaacaatcc ggctgccatt atccactgac cggtgtgccg
      901 gggtccggcg cgatgacatc accgggcgcc gggaccggtg tgttcgtctc cactggcgcc
      961 ggcgcggcgg ggcaccccga gatgcagcca cgatcgatcg cgacgttgtc gatcagcagg
     1021 tcaaaggtcc ggtccgtcgc ccaaccgccc aggactaccg tctgaagctc ggtcggggcg
     1081 gccacatcgg tcgcactgcc ggcgtactgc ccgttgaccc agatctggac cgtggccgag
     1141 ccggactcgg ccgcaaaggc gacttcgatg cggctccagt tggaggtgtg gatcgacccg
     1201 accgggatga gcgaatcgcc ttcgccgaat cggatgccca tcaacccatc gggacccagg
     1261 taaagcgccg cgatcgccgc gttgtcggct ccggtgatcg tcacgaagcg agttacgccc
     1321 gaatcgaggg ttcgcggcat cacgtcgagc gccacgtgga tattcgtttc gccggcagga
     1381 agtgcgcgtt gcaggtacga cggtgtgccg gaaaccacat cgctgccgga tgaacgcatc
     1441 tgcgcggcct gcgtatcgtc tcgccccgtg cctggtgcga gcaaagcgcc ttcgccggac
     1501 cagccggaca ggttgccctc gaagtcatcc gtgatcggca acgcggtttc cgggctcgca
     1561 acaggtgtcg gcgtctcgat cacctccgtc ggctccggcg atgcggattc ggtaggttgc
     1621 gcgaagggcg atgcctccgg gctgggctcc ggcgtggtgg tcggctcggg tggtacctcc
     1681 gccgtcgtca ccccgctcga ccagatgacc agccgcggct ggcgggatcc cgcctggcgc
     1741 gaataccacg agacggcgtc ggatgacgtg ccacggagga ggaaggtaac gtacccgtcg
     1801 cccgagatgg ccgatgtcac gtcataggcg atccaggagt tgtcggcgat cgggccgtag
     1861 ttgtcgaact ggctgcttgt cgggccggga cgagtgttcc aggtgaggcc ggtttcgcgc
     1921 cagctcgagc tggtcgtgta gatcgaggga ccgtcaactg tcgggtcgta ggcgtacagg
     1981 tagaggcggg cgcggtagac ccgatcgccg actccggaca ccttgaagcg aatcagcgtc
     2041 tcgtaatccg acgaggtgcc gcccttgacg ctcaggccgg tgccgagacc ttcattgcgc
     2101 gtcggattag cttcggacac ccgggcgtcg gcatggggcg cgaacgcatt gcccggcagg
     2161 aggttgaagg tcgccgtcac ggtcgagtcc gatgccggcg cgacgtactc atgtgatcgc
     2221 cggccaccgt ccgaccacga cttgtagatg tacggcgcat cgtccgggcc gatctggtcg
     2281 ggcgccacta cggtcaacgg gttgttcgcc caggtcagga ccgttgccgg ggcgcggtac
     2341 gggctgcctt caatccagag cagcatcccg ggcacgttcg agcccacagt tacgttgacc
     2401 gtgtttggca tcagattcat cgtgaccagc ttgccgagtc cgccggagtc gcgcacggtc
     2461 aacttgatct cgaggtagct ggttccggca gccttcaggt cttcgggagc gggcgcctgg
     2521 aaggtcaggt tattgccctt taccgggccg aaccaggggt gcgtgtgggt gtcgtggtga
     2581 agcagtacct cccacgtcat ccggctggat gaaagcgcgc cgtcctcggt atccgtgccc
     2641 aggccggtca gggtgatcat ctggctgacg ctgaactgtg acgaagccga tggcgaggtg
     2701 atctcggctg agggtcgcgt gttgccggcg ttgatcgtga cagcgtcggt gtcagtgccg
     2761 cccttgccat ccgtgacagt cagggtgacc cggaatttct tcgccgtttt gtaggtcttg
     2821 ctgaaggtcg agcgggtcga gctggtgccg tcgccgaagt cccatcggta cgtgagcggg
     2881 tcggcgtcgg gatcggagct tccgcgacct tcgaagccga ccgtcagtgg gacagccccg
     2941 aaggtcgggg ccgccttgat atcagcgacc ggcgcctgat tattggcggc gttgtaggag
     3001 atacggcgga tctgactgct gccgtaatcg gcatagtaga gcgcgacatt gctgccgtac
     3061 gggccgaatt cgagctcggt caccgcgccg agaccgctcg cgaatgtcgt gcgcgaccac
     3121 gttccaccgg agttcgtcag ggtccagatc gtgccgcagg tgtaatcgga gaagaaatac
     3181 gtcccgtcgt acgtcgacgg ccacacgccg ttggggatga aggcgccgcc cgacaaggag
     3241 ttgcaggacc cgtggccgta ctcgaagacc gggtcctgat cgacaaatcc gcagatggag
     3301 tttgagctgt tagcatggaa accttcgcag ttgttc
//
LOCUS       DASSGX010000275         3019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1141383, whole genome shotgun sequence.
ACCESSION   DASSGX010000275 DASSGX010000000
VERSION     DASSGX010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1141383"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..1575)
                     /locus_tag="VFI12_04810"
     CDS             complement(166..1575)
                     /locus_tag="VFI12_04810"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET7055761.1"
                     /translation="MAGEEFAYRSADELIEGYASGEFSPVEVTEAMLARIERLNPELN
                     AFTDVTADIARESAKHAEAEYRAGTAGPLSGVPVTIKDITFIEGLRCRRGSKIYADDA
                     PASFSSPFVQRVIEAGAVLLGQTTTPELGWKGETSSPLSCTTRNPWNVERTPGGSSGG
                     AGACVAAGIGTMAHGTDGAGSIRIPSAFSGIFGLKPSMGLVPVYPASAVGDLAYHGPM
                     TWWVRDAARMLNVVAGADARDRFSWNSGVDYLAALTDLDLRGLRVAWSRDLGYAAVVP
                     EVAALAEDASKVFAELGAEVVEDHPNLPDPWSIEDILWASAMAGARMDDFEQVQRIMD
                     PGLVEIVERGQKLTAAEMSRARTDQCIYAAAWAEWMRSYDLLLTPTLPCTAFPVGLNQ
                     PGTVNGVETSYLSWTAFTYPFNLAGLPAATVPCGFASDGLPAGLQIVGRLKDDALVLR
                     AAAAYEDARPWQGKRPPVS"
     gene            1689..2333
                     /locus_tag="VFI12_04815"
     CDS             1689..2333
                     /locus_tag="VFI12_04815"
                     /inference="COORDINATES: protein motif:HMM:NF039470.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6483 family protein"
                     /protein_id="HET7055762.1"
                     /translation="MQQDWLLRMIGQLSMFVSGIKKLRENKEWGEVHVQIGEGIGRFA
                     GLNASLVHAISEDDLIQLLSTRGGVDLDRWWALAELLREEGLALEEQGDAAGAERAFS
                     KSLRLYLEVLAEAEEIPSYLNVTDLEAVIERMLDRDLTRATRLELIQYLVATQRYDQA
                     ENVALWALDGPDIKAPQDARAFYESLRSKSDTELEAGGLSREEVEMGLANLTSE"
     gene            complement(2320..>3019)
                     /locus_tag="VFI12_04820"
     CDS             complement(2320..>3019)
                     /locus_tag="VFI12_04820"
                     /inference="COORDINATES: protein motif:HMM:NF013663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HET7055763.1"
                     /translation="MADAGVGAIITLGGDGTNRVVAKGCGEVPLVAVSTGTNNVFPRM
                     VEGTLAGLAAGLAAFGVADEAIARRPRLDVVVNDELRDIALIDVVTSPQQWIGARAVW
                     EPGHIREIMLSRIASGEIGICGIGGMIFPDAAGSSFGAHITVGAGPRVMAPLAPGIIR
                     EVPIADAVKVLPGESVRLQSAPGTIALDGEREIELLTDQDEIVVTLNPHGPFVVDIPG
                     AITQGSLAGLFTRS"
BASE COUNT          529 a         1082 c          954 g          454 t
ORIGIN      
        1 tggtcgctcc ccgcaggaac ttggtcgttc taccagcagg gctggcagag ctactactac
       61 gtcgacggca cgaccatcga cggcggcaag ccgtatgtct tcacgacaga tggatcaagc
      121 agcgccagcc gtctcgtcca ggacgagatc gtcgtcatcc agtaatcagg agaccggcgg
      181 ccgcttgccc tgccacggcc gggcatcttc gtacgcggcc gccgcccgca gcaccagcgc
      241 gtcgtccttg aggcgaccga cgatctgaag cccggccggc aacccgtcgc tcgcgaaacc
      301 gcacgggacc gtcgccgccg gcaacccggc caggttgaac gggtaggtga aggcggtcca
      361 gctcaggtag ctcgtctcga caccgttgac cgtgcccggc tggttcagcc cgaccgggaa
      421 cgcggtgcac ggtagcgtcg gcgtgagcag caggtcgtaa ctgcgcatcc actccgccca
      481 ggcggctgcg tagatgcact gatctgtgcg agcccgcgac atctcggcgg cggtcagctt
      541 ctggccccgc tcgacgatct cgaccagacc cgggtccatg atccgttgca cctgctcgaa
      601 gtcgtccatc cgcgcgccgg ccatcgccga tgcccagagg atgtcttcga tcgaccacgg
      661 atcgggcagg ttcggatgat cctcaacaac ctcggcgcca agctccgcga agaccttcga
      721 ggcgtcctcc gccagcgccg cgacctccgg cacgaccgcc gcgtaaccga gatcccggct
      781 ccaggcgacc cgcaaaccac gcagatcgag gtcggtcaaa gcggcgaggt agtccacacc
      841 cgaattccag gagaatcggt cccgggcatc cgcgcccgcg acgacgttca gcatccgcgc
      901 cgcgtcccgc acccaccagg tcatcggtcc gtgatacgcg agatcgccga ccgcgcttgc
      961 cgggtagaca gggaccagcc ccatgctcgg cttcagcccg aagatgccgg aaaaggccga
     1021 ggggatccgg atcgaaccgg ccccgtcggt cccgtgcgcc atcgtgccga tcccggccgc
     1081 gacgcaggcg ccggccccgc cactggagcc gcccggtgtc cgctcgacgt tccaggggtt
     1141 ccgggtcgtg cacgagagcg ggctgctcgt ctcgcccttc cagccgagct cgggcgtcgt
     1201 cgtctggccg agcaagaccg cccccgcctc gatcacccgc tggacgaagg gcgagctgaa
     1261 ggaggccggg gcgtcgtcgg cgtagatctt cgagccccgc cggcagcgca gcccctcgat
     1321 gaaggtgatg tccttgatcg tgaccggcac gcccgaaagc ggcccggccg tccccgcccg
     1381 gtattcggcc tcggcgtgct tcgccgattc ccgcgcaatg tcagccgtca catcggtgaa
     1441 ggcgttcagc tcggggttga gccgctcgat ccgggcgagc atcgcctcgg tcacctcaac
     1501 cggcgaaaac tccccactcg catacccctc gatcagctca tccgccgacc gatacgcaaa
     1561 ctcctcaccc gccactactc acctgccctc atccatcccg gtcatccgat tgcgccatcc
     1621 taccgggaac aggcggacgc ttgcgacacc cgcaacgacg ataatcggtg cctacaaagg
     1681 agcgcgaggt gcagcaggat tggttgcttc ggatgattgg gcagctgagc atgttcgtct
     1741 caggcatcaa gaagctgcgc gagaacaagg aatggggcga ggtccatgtg cagatcgggg
     1801 agggcatcgg tcggttcgcg ggtctgaacg cctcactggt ccacgccatc tccgaggacg
     1861 acctgattca gctcctgagc acgcgtggcg gcgttgacct cgaccgctgg tgggcgctgg
     1921 ccgagctgct ccgtgaggaa gggctggcgc tcgaggagca gggcgatgcg gccggggccg
     1981 agcgcgcctt ctcgaagtcg ctccggctct atctcgaggt gctggccgag gccgaagaga
     2041 tcccgtccta cctcaatgtg accgatctcg aagcggtgat cgaacggatg ctggaccgcg
     2101 atctcacccg cgcaacccgg ctcgaactga tccagtacct cgtcgcgacc cagcgctacg
     2161 accaggccga aaacgtcgcg ctttgggcgc tcgacggccc ggacatcaag gcgcctcagg
     2221 acgcccgcgc cttctacgaa tcgctcaggt cgaagagcga taccgagctg gaggcaggag
     2281 gactgagccg ggaagaggtc gagatgggcc tggcgaacct tacgagcgag tgaacaggcc
     2341 ggccagcgag ccctgcgtga tcgcgcccgg gatgtcaacc acgaaggggc cgtgcgggtt
     2401 gagcgtgacc acgatctcgt cctgatcggt cagcagctca atctcccgtt caccatccag
     2461 cgcgatcgtg cccggagcgg actgcagccg taccgattcg cccggcagca ccttgacggc
     2521 atcggcgatc ggcacctcgc ggatgatccc cggcgccagc ggcgccatca cccttggacc
     2581 ggcgccgacc gtaatgtgcg cgccgaagct ggagccggcc gcgtcgggga agatcatccc
     2641 gccgatcccg cagatgccga tctcgccgct cgcaatccgc gacagcatga tctcccggat
     2701 gtggcccggc tcccagaccg cccgcgcccc gatccactgc tgcggcgagg tgaccacgtc
     2761 gatcagggcg atgtcgcgca actcgtcgtt gaccacgaca tcgagccgcg gacgacgagc
     2821 gatcgcctca tccgccacgc cgaacgccgc caaccccgcc gccaatccgg ccagtgtgcc
     2881 ctcgaccatc cgcggaaaga cattgttggt cccggttgag acggccacga gcgggacttc
     2941 gccgcagccc ttggccacga cccgattcgt accgtcgccg ccgagcgtga tgatcgcgcc
     3001 gaccccggca tcggccatc
//
LOCUS       DASSGX010000276         2206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1359002, whole genome shotgun sequence.
ACCESSION   DASSGX010000276 DASSGX010000000
VERSION     DASSGX010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1359002"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VFI12_04825"
     CDS             complement(<1..205)
                     /locus_tag="VFI12_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055764.1"
                     /translation="MATVPENATILGSTIVFAPFSIEQALEGLAAAGYRNCEIGAVKG
                     WFEHIDPDTATDKEIADIKQRLND"
     gene            complement(217..1029)
                     /locus_tag="VFI12_04830"
     CDS             complement(217..1029)
                     /locus_tag="VFI12_04830"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7055765.1"
                     /translation="MPRAKVGDLDIAYDVTGEGDPVLMINGIGAPRGAWGLQVPAVSE
                     RYAAITFDNRDVGETGPGENAQRYSTDQFARDAAGLLNHLGIEKAHIVGASMGGCIAQ
                     AFATLYPERTQTATIICSWATVEPWLDELWEQWEGLFEARGPIEWNRTTWLWVFTHRA
                     YDEPGFLDNLLMSAHEDPHPQTLEMYLRQSHAARTHELLDRLGSVTAPAHVICGEEDI
                     FTPPRYSIEIANTIPGARLTVMPEVGHGMFWEATDDFNRTVLEFIDQHPLKG"
     gene            complement(1132..>2206)
                     /locus_tag="VFI12_04835"
     CDS             complement(1132..>2206)
                     /locus_tag="VFI12_04835"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET7055766.1"
                     /translation="PKPVFAEYQRWQLELERQPVEFLGRRYYDLMAESRAALAAYLNA
                     DPDDLIYVDNATAGVNVVVRSLELGPGDEIVTTDHEYGACELTWQFLQESRGAIIKRA
                     HIGLPLSTHEEIVDALWAQVTERTKVIYLSHITSFSALIFPVEEICRRAREAGIFTVI
                     DGAHAPGQIPLDMQAIGADCYAGNLHKWLCAPKGAGFLFVRPEHQDRMMAVIVSWGWG
                     EGRPEFGTTQFLRRNTWQGTRDPAAYLAVPAAIQFQAAHDWPAVRERCHELVVQARTR
                     ISNLTDLPPISPAGCDWFVQMATCPVRTKEIAALKAGLYDQYRVEIPCGEWQGMQYVR
                     VSIQAYNDGADIDRLMEGLGRLL"
BASE COUNT          346 a          707 c          770 g          383 t
ORIGIN      
        1 ggtcgttcag ccgctgcttg atgtcggcga tctccttatc agtcgcggtg tcgggatcga
       61 tgtgctcgaa ccagcccttg accgcgccga tctcgcagtt gcgatagccg gccgcggcca
      121 gcccctcgag cgcctgctcg atgctgaagg gcgcgaagac aatggtcgag ccgaggatgg
      181 tcgcgttttc cggaactgtt gccattcgct gtctccctat cccttgagcg gatgctgatc
      241 gatgaactcg agcacggtgc gattgaagtc gtcggtggcc tcccagaaca tcccgtgccc
      301 gacctccggc atgacggtca ggcgcgcgcc ggggatggta ttggcgatct cgatcgagta
      361 gcgcggcggg gtgaagatgt cctcctcgcc gcagatgaca tgcgccgggg cggtgaccga
      421 gccgagccgg tcgagcagtt cgtgggtgcg ggcggcgtgg gactgtcgca ggtacatctc
      481 gagcgtctgc gggtgcgggt cttcgtgcgc cgacatgagc aggttgtcga ggaaaccggg
      541 ctcgtcgtaa gcccggtgcg tgaagaccca gagccaggtc gtccggttcc actcgatcgg
      601 tccgcgcgcc tcgaacaacc cctcccactg ctcccacagc tcgtccagcc acggctcgac
      661 ggtcgcccac gagcagatga tcgtcgcggt ctgggtccgc tccgggtaga gcgtagcgaa
      721 ggcctgggcg atgcagccgc ccatcgacgc cccgacgata tgcgctttct caatgccgag
      781 gtgattgagc aggccggcgg catcgcgcgc gaactggtcg gtggaatagc gctgggcgtt
      841 ctcaccggga ccggtctcgc cgacgtcgcg attatcgaag gtgatcgccg cgtagcgctc
      901 gctgacggca gggacctgca agccccatgc gccgcggggc gcgccgatcc cgttgatcat
      961 caggaccgga tcgccctcgc cggtcacgtc gtaggcaatg tccagatcgc caaccttggc
     1021 ccggggcacc ggcgcaacct cctcaattga cttgaagcgg ggcgagccgc cccgcgctac
     1081 gtcctggtgg ttgcgccatt atcccggagg aatgcgattt tcgccggatg gtcagagcag
     1141 gcggccgagc ccttccatca ggcgatcgat gtcggccccg tcgttgtagg cctggatcga
     1201 gacgcgcacg tattgcatcc cctgccactc gccgcacggg atctcgaccc ggtattggtc
     1261 gtagaggccg gccttgaggg ctgcgatctc cttcgtgcgg actgggcagg tcgccatctg
     1321 cacgaaccag tcgcagccgg ccggcgagat cggcgggagg tcggtcaggt tcgagatccg
     1381 ggtccgcgcc tgcacgacca actcatggca gcgctcgcgg acggccggcc agtcgtgcgc
     1441 ggcctggaac tgaatcgcag ccggaacggc gaggtaggcg gcgggatcgc gcgtgccttg
     1501 ccaggtgttg cggcgcagga actgggtcgt gccgaattcg ggccggccct cgccccaacc
     1561 ccacgagacg atgaccgcca tcatcctgtc ctggtgctcc gggcggacga agaggaagcc
     1621 ggcccccttt ggcgcgcaga gccacttgtg caggttgccg gcgtagcaat cggccccgat
     1681 cgcctgcatg tcgagcggaa tctggcccgg ggcgtgggca ccgtcgatca cggtgaagat
     1741 gcccgcctcg cgggcgcggc ggcagatctc ctcaaccggg aagatcaggg cactgaagga
     1801 ggtgatgtgc gagaggtaga tgaccttcgt gcgctcggtc acctgcgccc agagcgcatc
     1861 gacgatttcc tcgtgcgtgg agagcggcag gccgatgtgc gcccgcttga tgatcgcgcc
     1921 gcgcgactcc tgcaggaact gccaggtcag ctcgcaggcg ccgtactcat ggtcggtcgt
     1981 cacgatctcg tcgccggggc ccagttcgag tgagcgaacg acaacgttga cgccggcggt
     2041 cgcgttgtcg acgtagatga ggtcgtccgg gtcggcgttg aggtacgcag cgagcgctgc
     2101 gcggctctcg gccatcaggt cgtagtaccg gcggccgagg aactcgaccg gctggcgctc
     2161 cagctcgagc tgccagcgct ggtactcggc gaacacgggc ttggga
//
LOCUS       DASSGX010000277         4360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1577440, whole genome shotgun sequence.
ACCESSION   DASSGX010000277 DASSGX010000000
VERSION     DASSGX010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1577440"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /locus_tag="VFI12_04840"
     CDS             <1..836
                     /locus_tag="VFI12_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET7055767.1"
                     /translation="QMAQQYIGADFDRIVVHHGDTGNTPQGNGTMGSRGLPVGGAALV
                     RSLNKIKEKAAQIAAHMLEAAVDDIEMVDGKYQVKGVPDRGVSLGDIAGKAYSGDLPD
                     EIEAGLETTDFFKPADETFPFGTHVSVVEVFPETGEIKLLRHVCVDDCGNIITPFLVT
                     GQVHGGLAQGIGQVILEELHYDDSGELITGTLNDYAVPRAHHFPMFESHHTTTTTPLN
                     PMGAKGIGEAATIGATPAAANAVIDALSHWGIHHLDTPFTPEKIWRAINEASATNSAA
                     D"
     gene            complement(1394..1654)
                     /locus_tag="VFI12_04845"
     CDS             complement(1394..1654)
                     /locus_tag="VFI12_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055768.1"
                     /translation="MKSAIFFAATRASQPSIRQITSAEAVVRLVQQSASEQLDRAGPS
                     EGAVAIVNNITCFELTPSDLDSTVKAILIHMGVDWPFSGSAA"
     gene            complement(3992..>4360)
                     /locus_tag="VFI12_04850"
     CDS             complement(3992..>4360)
                     /locus_tag="VFI12_04850"
                     /inference="COORDINATES: protein motif:HMM:NF026049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HET7055769.1"
                     /translation="ASADFSLATDAADLLAKRGVPFREAHEVVGRLVRTCTDSGRGFE
                     DLTVEEWAAAHPVFAEERPPLTAAESVAARDIPGGTAPNRVRAALGAARERYSDVRAW
                     HAREDHRLANVMKRDSTGPE"
BASE COUNT          905 a         1353 c         1312 g          790 t
ORIGIN      
        1 cccagatggc gcagcaatac attggcgctg atttcgaccg gatcgtcgtc catcatggcg
       61 acaccggcaa tacgccgcaa ggcaacggca cgatggggag ccgcggcctc ccggtcggcg
      121 gcgcggcgct cgtccgctcg ctgaacaaga tcaaggagaa ggccgcgcag attgccgcgc
      181 acatgcttga ggcagcggtc gacgacatcg agatggtcga cggcaagtat caggtcaagg
      241 gcgtgcccga tcggggcgtc tcactcggcg atattgccgg gaaggcctac agcggtgatc
      301 tgccggatga gatcgaggcg ggtctcgaga cgaccgactt cttcaagccg gccgacgaga
      361 cgttcccctt cggtacgcac gtgtcggtcg tcgaggtctt cccggagaca ggcgagatca
      421 agctgctccg ccacgtctgt gtagacgact gcggcaacat catcacgcca ttcctggtca
      481 ccgggcaggt ccatggtggc cttgcgcagg gcatcggtca ggtgatcctc gaagagctgc
      541 actatgacga cagcggtgag ctgatcacgg gcacactcaa cgactacgca gtgccgcgcg
      601 cgcaccactt cccgatgttc gagtcgcacc atacgacgac gacgacgccc ctcaatccga
      661 tgggcgccaa gggcatcggc gaggcggcaa cgatcggcgc aacgcccgcc gcggccaacg
      721 cggtgatcga cgcgctctcg cactggggca tccaccacct cgatacaccc ttcacgccgg
      781 agaagatctg gcgcgcgatc aacgaggcct cggccacgaa ctcggccgcc gactagtccg
      841 agaatgaggc caaatcaagc aagggccggg gccaaacccc tgcccttgct tttccaaagg
      901 tgcccgggag tcgatcgcca catacgctgg tactggcggc ccaggccgtc actcggccaa
      961 gggcaagggt tgagaccgta cggccaccgg aaccgtcctc acatcgacct cgcgcccatc
     1021 ttttcattag acgcgatgaa cacgagtacg ttgaccggta atccggatcg ggccacggct
     1081 aagcgccacc aagccccggc ttcatctgct ccaactgatt caccttcctc gccccaatcg
     1141 cggtgcgctg acgacgatca ctttcggcga atctctgcat gctgctggcc gggaacgcct
     1201 ccgtcacctc gtttctggcg accggcgtgg ggcgtggggt gggcaacacc gcaacatgtt
     1261 gtggttaccg ataattagtt ggctcaattg accccggctt acgcttgtat tgcctcgcaa
     1321 tcccaacttc aggtaccgca aacgaatgct ggcgcttcat cgccctcgga gctcctgact
     1381 ggaacttgct cccctacgcc gcagaacccg agaagggcca gtccaccccc atatgtatga
     1441 ggatcgcttt taccgtggaa tcgaggtccg agggagtcaa ttcgaagcaa gtaatgttgt
     1501 tgacgatcgc gaccgcgccc tcagagggac cggcgcggtc aagttgctct gaggcggatt
     1561 gctggacaag gcgtacgacg gcctcggccg aagtgatctg ccggatcgat ggctggctgg
     1621 cgcgagttgc cgcgaagaaa atggcagact tcacccgcgg tcccggttgc tcggcagggc
     1681 aaaatccggg gagaatcgtg cggtcgatgc tctcaaaaag cctcctgctt gccgggatac
     1741 gaaggccccg agctcgtaac attgctcgag atcttcgatc caacttaatt ggtcgcggaa
     1801 cgctcagcgc ccggaagctg ctccgctcaa cgagcgcgac atcgtcggtg tagacatcca
     1861 ggccggctag accgagcgcc atggtagtag tgctcttacc ggcgcctgac gggccaatga
     1921 gcagagcgca ccgatcacct gttccgacta gaccggcatg cacatgcacc gcgtgcgtcc
     1981 attgcttgag agcctgggag acgagtaacc actcggctcg tatcgctgcg gcgcctcgtt
     2041 ttgtagattc cggaatcctg cgctcaccgt ctgacactat ccacgagggg tatccggact
     2101 ctgcaggctc aatggtcagg tggagattcg gctcggaatt aggcaacgcc cgtggataac
     2161 cggcgagcac gatcgacagc gtcgaattca gcgaccaggg agccgtcacc tcagccgtta
     2221 gtccgtgaag ggaaatccgg tgcgttacga gtgttgaacg cactggccgc caaccgaacg
     2281 gtgatcactg gtcagtttgg aagtacgacg ggaatcaagt gaagcagcac cgcggataat
     2341 caggtcgtgg aacaaaggta tctccaggaa tctcaccgca tagtcgccga tgccttcgat
     2401 gctgactttg gtcaaatcat ccctgcttag cgcatttcga tcgctaacat cgttcatcat
     2461 tggtcacgag accatacgaa catacgttgt tcctcaagac gaccacatct tgaccggctt
     2521 ccaaacgcat cgcctgggtc atcactcata ttcaacggga acgcgctcgt cgagttgaca
     2581 cgcgaccccg gtgattacgc tggagcttga attgaggcca actgctcaag cagggcttca
     2641 actgatgggc cgccgaacat acggaagatg accgtaccgg attcgtcaac gattactgtc
     2701 aaccgccgag ggacgtcagt gccgggaagg agcgctccat acgcggcatg aacattgttc
     2761 tcactatcga tcacgaccgg gtacgggagc gaaagggaat ccaggaattc gctcgccagg
     2821 agccgctcgc gagcgataat cgcgaggggc tcaaaaccga gagagcggat ctgatgtatg
     2881 tgttcggtga cgactcggga cgggattcga tcgccgcaac accctctgag cccgctcggg
     2941 taaaacagca ggagcgtgcc gcgagggccg cgaacttgtt caagttcaac gggcttgccg
     3001 cgcgtcccag tcagcctgaa gctgggagcc aaatccccac ggtccatcat cacgcgttct
     3061 ccagtttctg aataacgtca ggatagaaag gagaggtgcc caatggcacc tctccgaagc
     3121 cgatctgagg cgaactattg gactatccgc cgatagtgcc ctgaccagtg gactggcgca
     3181 catcagcacc accagcatca ccgagcgggc actggcccgt cagggacgtc gggacgcaga
     3241 ccggatcgat gcaacaggta tcgattgcgc acacactgcc atccgggcac gcgttattcg
     3301 cgtcacacgg tccgatgatg ctgcatgacg tgctgcccgg cacgcagaat ccaccgacgg
     3361 gcgtggtaat acagacgcaa tccccattgc tggaactaca ttggataaag gatgcacagg
     3421 tagcgcctac gcactccgga tgttggttgc taatgctctg cgccatcgcg gactgcagct
     3481 tgaagctggc cgctacaagt ggcgcagcgt acgcaatctt cgcgccggtt ttaacgatcg
     3541 tgcgccgagt cgtctgagca tcaagctgtt ggactacccc gtctaacgcc atcgatgacc
     3601 accctttcgc gggtttcggg caaagcaccg gccaccatgg ccggatccgt tgcgggcacg
     3661 cgcccgttgt caggaaacac gcatacgtta tgacctgcat ccagagctgt caattgaacg
     3721 tacacctcga atcttcactg ttgcaatagt ctatttgcca gtccgcttgg cgggcgctcg
     3781 cgggccaaac ttggcgatga agctatttcc tcgctcgccg acgcgcacgc cccaggcgtg
     3841 acgcgcatgg gaaatgggat gcggtgtttc gagggccagc accggcacac gtgaaaccag
     3901 acaacgttgg aattcacgct gccggagcca ggtgagaatg ctaacaaggt ttgggttcac
     3961 aactgttgaa cggcggtcag caggagctcg attactccgg gccggtgctg tctcgcttca
     4021 tgacgttggc gaggcggtgg tcctcgcggg cgtgccaggc gcggacgtcc gagtatcgct
     4081 cccgggccgc accgagcgcc gcgcgaaccc ggttcggcgc cgtgccgccg gggatgtcgc
     4141 gcgccgccac gctctctgcg gcggtcagtg gtggccgctc ctcagcgaag acgggatgcg
     4201 ccgcggccca ctcttcgacc gtcaggtcct cgaacccgcg accggaatcg gtgcaggtcc
     4261 ggaccaggcg cccgaccacc tcatgggcct cgcggaacgg cacgccgcgc ttggcgagca
     4321 aatcagccgc gtcggtcgca agcgagaaat cggccgatgc
//
LOCUS       DASSGX010000278         2623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_486897, whole genome shotgun sequence.
ACCESSION   DASSGX010000278 DASSGX010000000
VERSION     DASSGX010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486897"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="VFI12_04855"
     CDS             complement(<1..660)
                     /locus_tag="VFI12_04855"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HET7055770.1"
                     /translation="MASALVTVLACGTDRSPRARAGSSESSAKADSFRVALLTPGPIS
                     DRSWNGGAYAGLVAIRDSLGATVSHVQTKTPAEFEENFRQYGAQGYNLVIGHGFEFQD
                     AAVRVAPDFPKTLYLTTSGNTVRANVAGLEFAFEEASYLAGILAAGMTKSGVIGAIGG
                     TELPPVKRSFAAYAAGAHSANPQVAIVTSYVGNWDDVSAGKEQALAQVARHVDVIFQN
                     AD"
     gene            complement(793..1254)
                     /locus_tag="VFI12_04860"
     CDS             complement(793..1254)
                     /locus_tag="VFI12_04860"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HET7055771.1"
                     /translation="MSIFTKPTTEKGLMRNDAPPPEAGLSVIASGMKIVGDIESTGVV
                     KIEGIVEGAIRGARQLLLGRQGTVHGDIRAHEVVIGGTVVGTIVADERVEIQGTSSVQ
                     GDIHTKSIVVLEGGVINGTVRMGDTAAREGAREPRDANADNPVRPGLALSQ"
     gene            complement(1818..>2623)
                     /locus_tag="VFI12_04865"
     CDS             complement(1818..>2623)
                     /locus_tag="VFI12_04865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HET7055772.1"
                     /translation="FRKIAIQQIKPNPFQPRKEFRDEELAQLQASLDANGLLQPVTVR
                     PVGNHYELIAGERRLRAATNLGWTEIPAIVRQYDDKALLTLALVENLQRSDLNPIEEA
                     EGYSRLVAEFDLTQQDVATVVGKDRSTVANALRLLNLPAVVRRLLQEGRLSVGHARAL
                     LAIPTERVMVDLAREAVAKNLSVREVERRVKQATTSARQPTAAPEKNGAARAAELRRL
                     TDRLRRRLQTDVAVDIDDKDRGQLRINFYSADDLNRLIELITGHTTEDV"
BASE COUNT          480 a          804 c          800 g          539 t
ORIGIN      
        1 gtcagcattc tgaaaaatca catcgacgtg tctggcgacc tgcgcaaggg cctgctcttt
       61 cccggcgctc acatcgtccc agttcccaac atacgaggtc acgatggcga cctgcgggtt
      121 cgcggagtgc gcaccggccg cgtacgcggc gaaactccgc ttgactgggg gcagttccgt
      181 cccgccgatc gccccgatca cgcctgattt ggtcatccct gccgcgagga ttccggccag
      241 gtaagacgct tcttcgaatg caaactccaa cccggcaacg ttcgcgcgca cggtgttacc
      301 cgaggtcgtg agatagagcg ttttgggaaa gtcgggagcg acgcgtactg cggcgtcttg
      361 aaattcgaag ccgtgcccga tcactaggtt gtaaccttga gcgccgtact ggcggaaatt
      421 ctcttcaaac tcggccggcg tcttagtctg gacgtggctt accgttgcgc ctaatgaatc
      481 gcggatcgcc acgagcccgg cgtatgcacc gccattccac gagcggtccg agatcggacc
      541 cggggttagc agcgcgaccc ggaacgagtc tgccttggca gagctctcac tggagccggc
      601 tcgggcacgc ggcgagcgat cggtgccgca agccaggacg gtgacgagcg cgctcgccac
      661 gctgatgccg gcgatggaac gacgggccaa cagggaattc ataagttgta gtcgcgcgac
      721 aggatgcctg atctgctcaa tcagacgttt gcgggcttct gagctttacc cgtgcgctgc
      781 tgccgcgcca aactactggc tcagagccaa gcccggccgg accggattat ccgcgttggc
      841 gtccctgggt tcccgcgcgc cctcgcgagc ggccgtgtct cccatccgca cggttccgtt
      901 gatcacgccg ccttcgagga cgacgatcga cttcgtgtgg atgtcgcctt ggacgctcga
      961 cgtgccttga atttcgacac gctcgtcggc gacgatcgtt ccaacgacag tccccccaat
     1021 gaccacttcg tgggcccgaa tgtcgccgtg cacggtcccc tggcgtccga ggaggagctg
     1081 tcgcgcccca cgtatggcgc cttcgacgat gccttcgatc ttgaccaccc cagtgctctc
     1141 gatatcgccg acgatcttca tccccgacgc gatgacggac aatccggctt ccggtggcgg
     1201 cgcatcgttt cgcatcagac ccttctctgt tgtcggcttc gtgaagatcg acattgcggc
     1261 tacctacagc ggtgacaggc ggacaaacga gtgactggtg agcaacgtgg cctaacgagt
     1321 ggcggtccca ctgtctgccc ggggcgactc cgtcggtggc gcaggcggac gcttggcgtt
     1381 aggtccgacg cgcgccgcgg ccctcgacgc tgcggcgggc gacggagtgt ttgcggaatc
     1441 gggccgtacc acggaagtac cgccggccgc gagcatctgt cgaacctgct cgtagcggct
     1501 gtgcatgcgc gagagggcga cctgaagcga atcgatgcgc cggttgtcgc gctccaattc
     1561 cgcgatgcga acagccagga cggggacgcg tacgctctgg actgcaaaga gtagccacgt
     1621 gccgataaat acggccaccg cagcgaggcc caagcgcgaa cctatcaacc ggacgagcgc
     1681 gacctggcgt tcgctgataa cgatagtccg gttggcgagc ccggtttcac gttgcacttg
     1741 gatgatcatg gaacctgcgt gcggagtgta gatattcccg tagccattcc ggagccggtt
     1801 catgaatcca aggcgcatca gacgtcctct gtcgtgtgtc cggtaatcag ttcgatgagc
     1861 ctgttgaggt catcagccga atagaagttg attcggagtt gccctcgatc cttgtcatcg
     1921 atgtctactg ctacgtccgt ctgcagccgg cgccggagac ggtcggtcaa gcggcggagc
     1981 tctgccgcgc gggccgcccc gtttttctct ggagctgcag tgggttgacg cgctgaggtg
     2041 gtcgcctgtt tcactcggcg ctcaacctca cgtaccgaaa gattcttggc aaccgcctct
     2101 cgcgcgaggt ccaccatcac acgttccgtc ggaatggcaa gcaaagcacg cgcatggccg
     2161 acggaaagtc gtccctcttg aaggagacga cggacaacag caggtaaatt caatagccgc
     2221 aatgcgttag ctacagtcga ccgatctttg ccgaccaccg ttgcaacgtc ttgctgcgtc
     2281 aagtcaaact cggcaactag cctgctgtag ccttcggctt cctcaattgg attcaagtct
     2341 gagcgctgga gattttcaac gagcgcgagt gtgaggagtg ccttgtcgtc gtactggcgg
     2401 acaatcgcgg gaatctcagt ccatccaagg tttgtggcgg ctcgaagcct ccgttctcca
     2461 gcgatgagct cgtagtgatt acctacaggc cgtacagtaa ccggctgtaa tagtccattg
     2521 gcgtcaagac tcgcttgtag ttgtgcgagc tcttcgtctc gaaactcttt gcgcggctgg
     2581 aacggattcg gcttgatttg ttgtatcgcg atctttcgga acg
//
LOCUS       DASSGX010000279         2511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1141526, whole genome shotgun sequence.
ACCESSION   DASSGX010000279 DASSGX010000000
VERSION     DASSGX010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1141526"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /locus_tag="VFI12_04870"
     CDS             complement(<1..1011)
                     /locus_tag="VFI12_04870"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET7055773.1"
                     /translation="MRLASRMQRLGTETAFEVLVRARALEAQGRDVVHLEIGEPDFDT
                     PDNVCDAGITAIQEGWTHYGPAAGQPDLRQAIVDYLNRSRGTAYVPGNVVVTPGGKPI
                     MFFVILALLEEGDEAIYPDPGFPIYRSMIDFTGARAVPLPIREENDFRLDVDELASLI
                     TPRTKLLIINSPANPTGGVLTRDDFAAIARLAVEHDLIVLADEIYAEIIYSGEHHSIS
                     TFPGMAERTVILDGFSKTYAMTGWRLGYGVMPEELADQVSRLMVNSVSCTASLVQRAG
                     LEALTGPQDAVRDMVAAFRRRRDLMVQGLNQIPGISCLEPAGAFYVFPNITGTGMTSK
                     AFA"
     gene            complement(1154..2335)
                     /locus_tag="VFI12_04875"
     CDS             complement(1154..2335)
                     /locus_tag="VFI12_04875"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HET7055774.1"
                     /translation="MENAVMTTGPFRVVRWLVAALMIVLAALPLSGDALAQGSPSLDV
                     VSPQDGATITANDIPVQVKVSDLKLDCAALGRPDENGVGQVLALIDGTTIAQLANFYC
                     TDTFTISGEGLAPGKHTLTFVLASNTHAPMMDTAKQVMVDFQPPQPAPLPAANYTGDP
                     GVTLSSPHDGDTVPTSFPVQITPVNFSPTAALEGKTNVPGYGHYHVWVDAPEKPASLA
                     NLVLMPGTNAFTLDLSAWGPGKHTIRIEPAQNDHTMYDPAQPATFTVTVSDTATPTAM
                     ASPAASPAAAAASAPTVNMTDELRFVPADLTIKVGDTVTWVNASAIQHTSTDDPAQNP
                     VASAHPEYAQLPSGAEPWNSGLLQPGQSFAQEFTVPGTYHYFCIPHVLSGMRGTITVQ
                     S"
BASE COUNT          414 a          751 c          899 g          447 t
ORIGIN      
        1 ggcaaaggct ttgctcgtca tgccagtgcc ggtgatattg ggaaagacgt agaacgctcc
       61 ggcgggttcg aggcaggaga tgccggggat ctgattgagt ccctggacca tcagatcgcg
      121 gcggcggcgg aaggcggcga ccatgtcgcg gacagcgtcc tgcggcccgg tgagcgcttc
      181 cagcccggcg cgctgcacga gcgaggcggt gcaggagacg ctgttgacca tgaggcggct
      241 gacctggtca gccagctcct ccggcatcac gccgtagccg agccgccagc ccgtcatggc
      301 gtaggtcttg gagaagccat cgaggatcac cgtccgctcg gccatgccgg ggaacgtcga
      361 gatggagtgg tgctctccgg agtaaatgat ctccgcatag atctcgtcgg cgaggacgat
      421 gaggtcgtgc tcgaccgcga ggcgggcgat ggccgcgaag tcgtcgcggg tgagcacgcc
      481 accggtcgga ttggccgggc tattgatgat gaggagcttc gtgcgcggcg tgatcagcga
      541 cgccagttcg tcgacgtcga ggcggaagtc gttttcctcg cggatcggca agggcaccgc
      601 gcgggcgccg gtgaaatcga tcatgctgcg gtagatcggg aaccctgggt cggggtagat
      661 agcctcgtca ccctcctcca gcagcgcgag gatgacgaag aacatgatcg gcttgccgcc
      721 gggagtaaca acgacattgc ccggaacgta ggccgtaccg cggctgcggt tgaggtaatc
      781 gacgatagcc tggcgcaggt ccggctgacc cgcggccggg ccgtaatggg tccagccctc
      841 ctggatggcg gtgatgcccg cgtcgcagac gttgtctggg gtgtcgaaat cgggttcgcc
      901 gatctccaga tggacgacat cccgtccctg ggcttccagg gcgcgggcgc gcacgaggac
      961 ttcgaaggcc gtttccgtgc cgagccgctg catccgactc gcgagtcgca tctctcacct
     1021 ccaggattgc cgggtcgttg tttcgccgca agcataccgg aggagcgttg acgagctatg
     1081 ccggagagcg cagtcagggc agagatcgga tcccgcaacg ggcagataca tccgatccct
     1141 gcccccggag gcgtcagctc tggaccgtga tcgtgccacg catcccggag agcacgtgcg
     1201 ggatgcagaa gtagtggtag gtgccgggga cggtgaattc ctgcgcgaac gactgacctg
     1261 gctgcagcag acccgaattc cacggctccg ccccggatgg caactgcgcg tactccgggt
     1321 gtgccgaggc gaccgggttc tgggccgggt catcggtcga ggtgtgctgg atagcgctcg
     1381 cgttgaccca ggtcaccgtg tcgccgacct tgatcgtcag gtccgcgggc acgaaccgga
     1441 gctcgtccgt catgttgact gtcggcgcgg aggccgctgc ggctgctggg ctggcggcgg
     1501 gactggccat cgccgttggc gtcgccgtgt cgctcaccgt aaccgtgaac gtggccggct
     1561 gggccgggtc gtacatggtg tggtcatttt gcgctggctc gatgcggatc gtgtgcttgc
     1621 cgggacccca ggcgctgagg tcgagcgtga aggcgttcgt gcccggcatc agcaccagat
     1681 tggccaatga cgcgggtttc tccggcgcat cgacccagac gtggtagtgg ccatagccgg
     1741 gaacgttcgt cttgccttcg agcgcggcgg ttgggctgaa attgaccggg gtgatctgga
     1801 ccgggaagct ggtcgggacg gtgtcgccgt catgaggact ggagagcgtg acgcccggat
     1861 cgcccgtata gttcgctgcc ggcaggggcg ccggctgtgg cggctggaaa tcgaccatga
     1921 cctgtttggc ggtgtccatc atcggcgcgt gggtgttgct ggccaggaca aatgtcagcg
     1981 tgtgcttgcc cggcgccaag ccctcgccgg agatggtgaa cgtatcggtg caatagaagt
     2041 tggcgagttg ggcgatggtc gtgccatcga tcagggcgag cacctgaccg accccgttct
     2101 catcggggcg acccagcgcg gcgcagtcga gcttcagatc gctcaccttc acctggacgg
     2161 ggatgtcgtt cgctgtgatc gtggcgccgt cctggggaga gacgacatcg agcgacgggc
     2221 tgccctgggc cagggcgtca cccgagagcg gcagcgccgc cagcacgatc atcagcgcag
     2281 cgacgagcca tcgcaccacc cggaacggtc ccgtggtcat aaccgcgttt tccatttcca
     2341 tgctccatga cggtcccact cgtgacgcca catcgcgccg gacaatcgac caaggcgagg
     2401 tctgcatacg ccatgccatg cggatcaggt tcggagccac tccggtaggg atggcccgct
     2461 gccgcgcatt tggcgctgtt gactgggtca atcgggcagc gtagagtcac g
//
LOCUS       DASSGX010000280         4667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1248857, whole genome shotgun sequence.
ACCESSION   DASSGX010000280 DASSGX010000000
VERSION     DASSGX010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1248857"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /gene="rpsB"
                     /locus_tag="VFI12_04880"
     CDS             <1..780
                     /gene="rpsB"
                     /locus_tag="VFI12_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HET7055775.1"
                     /translation="VHFGHQKKRWNPKMRNYIFTEKNGIHVIDLNQTVNLLNDAYQFV
                     TNVTASGGRILFVGTKKQAQEVVAREATRSGQFYVNRRWLGGTLTNFVTIRSRLRTLK
                     QLQSEAASGGFERLPNQEAATKRQELERLERTLGGMRDMTSLPGAVFVIDPKREHLAI
                     QEARRLHIPVIAITDTNCDPDLVDYIIPGNDDAIRSVRLISAAIADAAIQGSMQAEIA
                     VAEQEMDMAGADADAAAAAIVAAEAPAEVETPAPADETAAV"
     gene            817..1377
                     /gene="tsf"
                     /locus_tag="VFI12_04885"
     CDS             817..1377
                     /gene="tsf"
                     /locus_tag="VFI12_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HET7055776.1"
                     /translation="MVTTSLVKELREQTGAGIMDCKRALEQAGGDLKKAVDLLKQTGL
                     AKADKKSGRAALQGVIETYVHAGGRIASMVELNCETDFVARTPDFKTLAREIAMQVAA
                     MNPRHISADAVPDAEWAALESEFGDRKKAGEAVCLYEQPFIRDPKVSIRDLIRETVAK
                     VGENIQVQRFARFEVGADRTETGADE"
     gene            1409..2182
                     /gene="pyrH"
                     /locus_tag="VFI12_04890"
     CDS             1409..2182
                     /gene="pyrH"
                     /locus_tag="VFI12_04890"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HET7055777.1"
                     /translation="MLPYRRVLLKLSGEALMGDAPYGIDPTVAKSIAAQIKRVLDQEI
                     QMSVVIGGGNIWRGLAASSKGMDRATADYMGMVATVLNALALQDALEKAGVHTRVMTA
                     IEMHEVAEPYIRRRAIRHMEKGRVVILAAGTGNPYFTTDTAAALRALELDADVLLMAK
                     HGVDGVYSEDPRTNPEARRYRQVNHQDAIERNLRVMDTAALSLCRENGLPILVFDMSA
                     DASIFAAVTGSAVGTLVSSGETIFDEVVVAVVTESESPQ"
     gene            2179..2736
                     /gene="frr"
                     /locus_tag="VFI12_04895"
     CDS             2179..2736
                     /gene="frr"
                     /locus_tag="VFI12_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HET7055778.1"
                     /translation="MMKDAIANAEERMKKAMDALRRDFSTIRTGRASPALLDRVHVEY
                     YGADTPLNQLAGVSAPEARMLMIQPWDRGSIPAIEKAIQKSDLGLTPNNDGQVIRLSI
                     PPLTEERRKQLVKTVHAMVEDSKVSVRNVRRDAVHAVQKLTSEKQISEDEERRGITQL
                     DDLAKQYTDQASAIGKEKEHEVLEV"
     gene            2766..3494
                     /gene="uppS"
                     /locus_tag="VFI12_04900"
     CDS             2766..3494
                     /gene="uppS"
                     /locus_tag="VFI12_04900"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HET7055779.1"
                     /translation="MEPISAARIPRHVAIIMDGNGRWAAQRGLPRLAGHDAGTENIRR
                     ITFKAGELGIRYLTLWAFSTENWRRPPDEVQGIMQILARAIESETQELHRQGAQLRHI
                     GDLSALDPELQQAVRDAIELTRDNDRLVLTLAFNYGGRQELIHAIQQIIADGAQPGQV
                     DEALVDQYLYTAGTPDPDLVIRTSGECRMSNFLLWQASYAELYFSSVLWPDFGPDELE
                     AAVREFGKRERRFGHISAAALTAE"
     gene            3498..4367
                     /locus_tag="VFI12_04905"
     CDS             3498..4367
                     /locus_tag="VFI12_04905"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HET7055780.1"
                     /translation="MRQRSISAVGVVFAGIVPALIGGPAWAIAIALFTGVGLYEYNRL
                     ADGAGGRTTHAGYVAVFGACLVAAYGGSVTLTMLPLALAVGLVFSLVLLRGNAPGSLN
                     TIAFDLSGALYLAIPAFAAIALRRVEGTISRDWLTSTADFLSPSWNAAERGLAWLLFV
                     IVVTWLSDTGAYLVGRSFGKTPLVPAISPKKTVEGLAGGLIAAVLFGLLANWLFGLGL
                     PVLAAAGAALLLALFGVVGDLAESLLKRQAGVKDSGTLIPGHGGMLDRIDALLFTWTA
                     GLYLAHLCDRIWA"
     gene            4364..>4667
                     /locus_tag="VFI12_04910"
     CDS             4364..>4667
                     /locus_tag="VFI12_04910"
                     /inference="COORDINATES: protein motif:HMM:NF014700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055781.1"
                     /translation="MTMNSTAGLIRIALLGSTGSIGTQTLDVVRAHPDRFAVTAIAAG
                     SNADLLEAQVNEFRPMIASLDCAAEIELSSGTRLLRGAEGLLACATHSEVDVVVVAT"
BASE COUNT          857 a         1526 c         1471 g          813 t
ORIGIN      
        1 gttcacttcg ggcaccaaaa gaagcgctgg aacccgaaga tgcgcaacta catcttcacc
       61 gagaagaacg gcatccacgt catcgacctc aatcagaccg tcaacctgct caacgacgcc
      121 taccagttcg tgaccaatgt caccgcgtcg ggcgggcgaa ttctcttcgt cggcaccaag
      181 aagcaggcac aggaagtcgt tgcccgcgaa gcgacgcgct ccggccagtt ctatgtcaac
      241 cggcgctggc tcggtggcac cctgaccaac ttcgtcacca tccgctcgcg cctgcgcacc
      301 ctcaagcagc tccagagtga ggccgcatcg ggcgggttcg agcggctgcc gaaccaggaa
      361 gctgccacca agcgccagga actcgagcgg ctcgagcgga cgctcggcgg catgcgggac
      421 atgaccagcc tgcccggcgc ggtcttcgtg atcgatccca agcgcgagca cctcgccatc
      481 caggaggcgc gccggctcca catccccgtc atcgccatca ccgacacgaa ttgcgacccg
      541 gatctggtcg attacatcat cccgggcaac gacgacgcga tccgctcggt ccgcctgatc
      601 tcggccgcca tcgccgacgc cgcgatccag ggctcgatgc aggctgagat tgccgtcgcc
      661 gagcaggaga tggacatggc cggcgccgat gcggatgcag ccgctgcggc gattgtcgca
      721 gccgaagccc ctgccgaggt ggagactccg gccccggctg acgaaacggc cgccgtctaa
      781 cgctttcgaa ttcaatctgg aaggaagata ggaccagtgg ttactacctc tctcgtcaag
      841 gagctgcgcg agcagacggg cgccggcatc atggactgca aacgcgcgct cgagcaagcc
      901 ggcggcgacc tgaagaaggc cgtggacctg ctgaagcaga ccggccttgc caaggccgac
      961 aagaagtccg gccgcgccgc gctccagggc gtgatcgaga cctacgttca cgccggcggc
     1021 cggatcgcgt cgatggtcga gctgaactgt gaaaccgact tcgtcgcccg cacgccggac
     1081 ttcaagaccc tcgcgcgcga gattgccatg caggtcgcgg cgatgaaccc gcgccatatc
     1141 tcggccgacg ccgtgccgga tgccgagtgg gccgcgctgg aatccgagtt cggtgatcgc
     1201 aagaaggctg gcgaggcggt atgtttgtat gagcagccgt tcatccgcga cccgaaggtc
     1261 tccattcggg acctgatccg cgagaccgtc gccaaggtcg gcgagaacat ccaggtgcag
     1321 cgcttcgccc gcttcgaagt cggcgcggat cgcacggaga ccggcgccga cgagtagctc
     1381 gatcgaccag gtggaaggga atccgagggt gttaccgtat cgccgtgtcc tgctcaaact
     1441 cagcggcgag gccctcatgg gggacgcgcc ctacggaatc gaccccaccg tcgccaagtc
     1501 gatcgccgcg cagatcaagc gcgttctcga ccaggaaatc cagatgtccg tcgtcatcgg
     1561 cggcggcaac atctggcgcg gcctcgcggc cagcagcaag ggcatggacc gggccactgc
     1621 cgattatatg gggatggtgg ccacggtcct gaacgcgctc gcgttgcagg acgcgcttga
     1681 gaaggccggg gttcacacgc gggtcatgac ggcgatcgag atgcacgaag tcgccgaacc
     1741 ctacatccgc cgccgggcga tccggcatat ggagaagggc cgcgtcgtca tcctcgccgc
     1801 cggcacgggc aacccctatt tcaccaccga cacggcggcc gcactgcggg cgttggagtt
     1861 ggatgcggat gtgctcctta tggcgaagca cggcgtggac ggcgtctatt ccgaagatcc
     1921 gcgcaccaat cctgaggccc ggcgctatcg gcaagtgaac catcaggatg ccatcgagcg
     1981 taacctgaga gtaatggata ccgccgcgct ctccttgtgc cgcgagaatg gcttgccgat
     2041 tctggtattc gacatgagcg ccgatgcgtc gatcttcgcc gccgttaccg gctcggcagt
     2101 tggcacattg gttagttccg gcgagactat cttcgacgaa gtcgtcgttg cggtcgtgac
     2161 agaaagtgag agccctcaat gatgaaggac gccatcgcca acgccgaaga gcggatgaag
     2221 aaggccatgg acgcgctgcg gcgtgacttc tccaccattc ggacgggacg cgcgtctccg
     2281 gcactcctgg accgcgttca cgtcgagtac tacggcgccg atacgcccct caatcagctt
     2341 gccggcgtgt ccgccccgga agcccgcatg ctgatgatcc agccgtggga ccgtggctcg
     2401 atcccggcga tcgagaaggc gatccagaag tccgaccttg gcctgacgcc aaacaacgat
     2461 ggtcaggtca tccgcctcag cattccgccg ctcaccgagg agcgccgaaa gcagctcgtc
     2521 aagaccgtcc acgcgatggt cgaagactcg aaggtgtcgg tccgcaacgt tcgccgggac
     2581 gccgttcatg cagtccaaaa gttgacgtcc gaaaaacaga tttccgagga cgaagagcga
     2641 cgcggaatca cgcagctcga tgacctcgcc aagcagtaca ctgatcaggc ctccgcgatc
     2701 ggcaaggaaa aagagcacga ggtcctcgag gtctagcctc tttcccgggg gacctcagcc
     2761 aaacgatgga accgatttcc gccgcccgaa tacctcgtca cgttgccatc atcatggatg
     2821 ggaatgggcg gtgggctgcg cagcgcggtc tgccgcgcct ggcgggacat gacgccggga
     2881 ccgaaaacat ccgccggatc acgttcaagg cgggtgagct cggaatccgc tacctgacac
     2941 tctgggcgtt ctcgaccgag aactggcgcc ggccgccgga cgaggtgcag gggatcatgc
     3001 agattctggc gcgggcgatc gagtccgaga cgcaggagct gcatcgccag ggagcgcagt
     3061 tgcggcacat tggcgacctc agcgcgctcg atccagaact ccagcaggct gtccgcgacg
     3121 ccatcgagct cacccgcgac aacgatcgac tcgtcctgac gctggcgttc aactacgggg
     3181 gccgtcagga gctgatccac gcgatccagc aaataatcgc cgacggtgct caacctgggc
     3241 aggtcgatga ggcgctggtt gaccagtacc tctacacagc cggcacgccg gatcccgatc
     3301 tggtcatccg cacctctggc gagtgccgaa tgagcaactt cctgctctgg caggcatcct
     3361 acgccgagct ctacttcagt tcggtgctgt ggcccgattt cggccctgat gagctcgaag
     3421 cggccgtgcg tgagttcgga aagcgggaac ggcgcttcgg gcacatctct gctgccgccc
     3481 tcactgccga gtaagctgtg cgccaacgct ccatcagcgc ggtcggtgtc gtatttgccg
     3541 ggatagtgcc cgccttgatc ggcgggccgg cttgggccat cgcgatcgcg ctcttcaccg
     3601 gtgtcggtct ctacgaatac aaccgcctgg ctgatggcgc gggcgggcgt acgacgcatg
     3661 ccgggtatgt cgctgttttc ggcgcatgcc tcgtcgcggc gtatggcgga tcagtcacgc
     3721 tcacgatgtt gcccctggcc ctcgccgtcg ggctcgtctt cagcctcgtc ttgctccgcg
     3781 gcaatgcgcc cggctcgctc aatacgatcg cattcgacct ttccggcgcg ctctacctcg
     3841 cgatccccgc gttcgccgca atcgcgctcc ggcgggtcga ggggacgatt tcccgggact
     3901 ggctcacttc gactgccgat ttcctttcgc catcctggaa cgccgccgag cggggactcg
     3961 cctggctcct gttcgtgatc gttgtgacct ggttgagcga taccggcgcg tatctcgtcg
     4021 gccggagctt cgggaaaacc ccactcgttc ccgcgatcag tcccaagaag acagtcgaag
     4081 ggctggccgg tggcctgatt gcagcggtgt tgtttgggtt gctggcaaac tggctgttcg
     4141 gactcggcct gccggtcctc gccgccgccg gggccgcatt attgctcgcg ctattcggtg
     4201 tggtgggcga tctggccgag tcgctcctca agcgccaggc cggcgtcaag gacagcggca
     4261 cgcttatacc cggtcatggc gggatgctcg atcgcatcga tgcgctcctg ttcacctgga
     4321 cagcggggct ctacctggcg cacctgtgcg accggatttg ggcatgacga tgaactctac
     4381 cgccggactc atacgaatcg ccctgctcgg ctcaaccggg tcgatcggca cacagacgct
     4441 ggatgtggtg cgcgcccatc ccgatcggtt cgccgtaacc gctattgcag ccgggagcaa
     4501 cgcggacctt ctcgaagcgc aagtcaacga attccggcct atgatcgcga gtctcgactg
     4561 cgctgcggag atcgaactct catccggtac ccgccttctg cgtggagcag agggtttgct
     4621 cgcctgcgcc acgcattccg aggttgacgt cgtagtcgtg gcgacgt
//
LOCUS       DASSGX010000281         5217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_379231, whole genome shotgun sequence.
ACCESSION   DASSGX010000281 DASSGX010000000
VERSION     DASSGX010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5217
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379231"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..700)
                     /locus_tag="VFI12_04915"
     CDS             complement(188..700)
                     /locus_tag="VFI12_04915"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HET7055782.1"
                     /translation="MVTLIPHRANIEPALSPAERDALAGAIAIEGAQVGDLLAVAKLQ
                     RRCFRRSLAYRPTTLLMLLLWPRARFLVARAGRTIVGCVIGDVSGGQSRVISICVDPD
                     WRRRAIGSRLLAAIATALPEGNMILMVEAGNIGAQQMYRDAGYLPVGESRDYYGRGRN
                     GIWMQKTRPR"
     gene            complement(720..3098)
                     /locus_tag="VFI12_04920"
     CDS             complement(720..3098)
                     /locus_tag="VFI12_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055783.1"
                     /translation="MSCTLHRLRLGAIALLIAVLFAGTPLAPALAQSDPTETPTGDAP
                     TGVLTVNVTACTRDDQPEGTISIVPAGETDSLECQPGDSASLSIDGGDPSTIANGDTI
                     ELPVGSHTVTETTQGASLTFEISESAETAIEVTTAVAPAEAPAATETPGVSVTETGPN
                     TVTVVAHLCREGITRDDFAADRPWTDDVRDCPVLTLPDNYGDVPADVVSANDPDNPLP
                     FDVSLAYDTGDASAIVALADATFTPSNVCESDFGQNLNGVANDDRCWDLSGYQVNDVV
                     PGQVTVSVDTLPEGYTLGGATLDGGAAITTTDPDAATTTIDTSVTGDADVHLFFVPKP
                     ATNEITVISHLCGAGIDSRAKFNAIGDHWAKLNACPTIVGTGDAPAPDSVSAGAVDFT
                     VEVQPLNGTAQQLADGTFLQRKVCEADLPVDINGNPNDNVCLDLSKYDFADVTQGVVT
                     IRANKQLPPNAIFVGISTVPDSGDDAAVTGVGMTGTIKLDTTNDGHVTIHVFYGPPPP
                     ATAAPPTATATPTKAPTKAATNTPAPAGPTKTPTRTATPAPTKTPGGPTETPSATNTP
                     APQAGSLQIFKLWCEGDESLTRINALAPGQDATRSDLGDATCQSGNSDFVLFDGGGSQ
                     LQTVSVPAIGVLMVDNLPAGSYSIRDTRSSASGRFEIRAGTITKVISLHYQAAEEIPD
                     APIVPTVDLGEDGNPPDVEDPLPVDDETDFPFDGPVAIPDGSDPFTVVDDPEAEARVE
                     SVDSFDELPGVGIGQGLTGSDSPLLWFATLTGIVTLLGGARLRSRRFVRNRP"
     gene            complement(3549..4328)
                     /locus_tag="VFI12_04925"
     CDS             complement(3549..4328)
                     /locus_tag="VFI12_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HET7055784.1"
                     /translation="MSELNRWRSEPWVMLPTEPASPAMNLGLDEELTLRVGAGERPPT
                     LRFWSWTASCIVLGRFQSVRNEIQVENAQERGVEIVRRISGGGAMFIEPEGAITYSLY
                     APARIAEGMSFAESYAFFDSWVISALRSLGIDAWYAPLNDITSANGKIGGAAQARRGG
                     AILHHTTMAYQMNIPVMMEVLRIGQEKLSDKGIRSADKRVGPLRQQTNLPRETIIEHL
                     IAEFADLTDLRQDTLTDDERAGAETRVRERFATDAWRYILP"
     gene            complement(4321..4617)
                     /locus_tag="VFI12_04930"
     CDS             complement(4321..4617)
                     /locus_tag="VFI12_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--protein ligase"
                     /protein_id="HET7055785.1"
                     /translation="MHGAFKTPGGKLVQVDFDVEDERLRNVEVTGDFFLYPEEALTAI
                     TASVEGSPADMPIAERTATIAAAIPPGVEWLGSSPEALAAAIERALLASLDTYE"
     gene            complement(4632..5045)
                     /locus_tag="VFI12_04935"
     CDS             complement(4632..5045)
                     /locus_tag="VFI12_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055786.1"
                     /translation="MLDAFRELIDELLSAPAALRGAGLATTTDDGQRTTLALITERDA
                     ALLDRIQTTIRTNTPLLKAPPDAPSAATLDGTSEELMDRFDTGRGELVSLLMNLTLKD
                     WERTAIDWTGQEISVADDVEQHVEFDEQVRDELGG"
BASE COUNT          806 a         1645 c         1846 g          920 t
ORIGIN      
        1 ccacggggga ccgcgctacg atttggttcg ggtgcatgca aacgggccag atgagatgcg
       61 gcgggcagat gccatccgcc cctacccatg ttctcgccac cggcaaccgg cgggcggcgg
      121 cggattttcc ccgcaaatcc ctacgatgct gttcctgatc ccgaatccct gccccgtgcg
      181 gacgtagtca gcgtgggcgc gtcttttgca tccagatgcc gttgcggccc cgaccgtagt
      241 agtcgcggct ttcgccgacc gggaggtagc cggcatcccg gtacatctgc tgcgcgccga
      301 tgttgccggc ctcgaccatc aggatcatgt tgccctcagg gagcgcggtc gcgatcgccg
      361 caagcaggcg gctcccgatc gcgcggcgtc gccagtcggg gtcgacgcag atgctgatca
      421 cgcgggattg tccgccgctg acgtcgccga tcacgcagcc gacgatcgtg cggccggccc
      481 gggcgaccag gaaccgcgcc cgcggccaga gcagcagcat cagcagggtc gtcggccgat
      541 aggcgaggga gcggcggaag caccggcgct ggagcttagc gaccgccagg agatcaccca
      601 cctgagcgcc ctcgatggcg atggcaccgg ccagtgcatc ccgctccgcc ggagagagtg
      661 ccggctcgat attcgcgcga tgcgggatca gggtgaccat agtgcgctcg ccgaaatgct
      721 cagggacgat tgcggacgaa acggcgcgac cgtaaccgcg cgccgccaag cagggtaacg
      781 atgccggtca gggtcgcgaa ccagagcagc ggcgagtccg agccggtcag gccctgccca
      841 ataccgacgc ccggcagctc atcgaagctg tctacgctct caacccgtgc ctcggcctcc
      901 gggtcgtcga cgactgtgaa cggatcactg ccgtccggga tcgcgaccgg accgtcgaac
      961 gggaagtcgg tctcgtcatc aaccggtagc gggtcctcga catcgggggg attgccgtct
     1021 tcgccgagat cgaccgtcgg aacgatcggc gcgtccggga tctcctcggc ggcctggtaa
     1081 tgcagcgaga tgaccttggt gatggttccg gcgcggatct cgaagcgacc gctggccgac
     1141 gagcgggtgt cccggatgct gtagctgccg gccggcaggt tgtcgaccat cagcacgccg
     1201 atcgccggga ccgagacggt ctggagctgg ctgccgcccc cgtcgaagag gacgaaatcg
     1261 ctgttgccgc tctggcaggt agcgtcgccg aggtcgctgc gggtggcgtc ctggccgggc
     1321 gccagcgcat tgatccgggt cagcgactcg tcgccctcgc accacaactt gaagatctgg
     1381 agcgatccgg cctgcggagc cggggtattc gtggccgagg gtgtctcggt cggaccgccc
     1441 ggtgttttgg tcggggccgg agtggccgtc ctggtcggtg tctttgttgg tcccgccggg
     1501 gccggcgtgt ttgtggcggc cttcgtcggc gcctttgttg gagtggcggt cgcggttggt
     1561 ggcgcggcag tggccggtgg tggtgggccg tagaagacgt ggatggtcac gtggccgtcg
     1621 ttggtcgtgt cgagcttgat cgtgccggtc atcccgacac ccgtgacggc cgcatcgtcg
     1681 cccgaatcag gcacggtcga aatgccgacg aagatggcat ttggcgggag ctgcttgttg
     1741 gcccggatgg tgaccacgcc ctgtgtcacg tcggcgaagt cgtatttcga gaggtccagg
     1801 caaacgttgt cgttcgggtt gccgttgatg tcgaccggca gatcagcctc gcagaccttc
     1861 cgctggagga acgtgccgtc cgccaattgc tgggccgtgc cgttgagcgg ctggacctcg
     1921 acggtgaagt ccaccgcgcc ggccgagacg ctgtccggcg ccggcgcgtc gccggtcccg
     1981 acgatggtgg ggcaggcgtt gagcttcgcc cagtgatcgc cgattgcatt gaacttcgcg
     2041 cgcgagtcga taccggcgcc gcagaggtgg ctgatgacgg tgatctcatt cgtagccggc
     2101 ttgggcacga agaagaggtg gacgtcggcg tcaccggtca cgctggtgtc gatcgtggtc
     2161 gtggcggcgt cgggatcggt ggtcgtgatc gccgcgccgc cgtcgagcgt agctcccccg
     2221 agcgtgtagc cctcgggaag cgtgtcgacg ctgactgtga cctggccggg gacgacgtca
     2281 ttgacctgat agccggagag gtcccagcag cggtcgtcat tcgcgacacc gttcaggttc
     2341 tggccgaaat ccgattcgca gacgttgctg ggcgtaaagg tggcatcggc cagggcgacg
     2401 atggccgaag catcgccggt gtcgtacgcg agcgagacgt cgaagggcag cggattgtcc
     2461 gggtcatttg cgctgaccac gtcggccggg acatccccgt agttgtccgg caaggtcagg
     2521 accgggcagt cgcgcacatc gtcggtccac gggcgatcgg ccgcgaagtc atcccgcgta
     2581 attccctcgc ggcagagatg agcgacgacg gtgacggtgt tcggtccggt ttcggtgacc
     2641 gagacgccgg gagtctccgt tgcggccggc gcttcggccg gggcgacggc ggttgtgacc
     2701 tcgatggcgg tctcggcgct ctctgagatc tcgaaagtga gtgatgcgcc ctgggtcgtc
     2761 tcggtgacgg tgtgcgatcc aaccggcagc tcgatcgtgt cgccgttcgc aatcgtcgac
     2821 gggtcgccgc cgtcgatcga gagggaggcg ctgtcgcccg gctggcattc gaggctatcc
     2881 gtctcgcccg ccggtacgat gctgatcgtg ccttcgggct ggtcgtcgcg ggtacaggcg
     2941 gtgacattga cggttagcac gccggtcggc gcgtccccgg tcggtgtttc ggtcggatca
     3001 ctctgggcca gggcgggagc aagcggcgtt cccgcgaaca ggacggcaat gagcaatgcg
     3061 atcgcgccga gccgcagccg gtgcagggta caagacatga aaagggccct cgcaggcggg
     3121 gtcggatggt gcatcgcaga gataccagcc acgagcgatg cgtccggggc cgccgtgtgg
     3181 tcgtgccgtt caggatagca acccgaattt ggatagtcga cacatgccgg ggcccgatgg
     3241 gcctatccgg cggtcaactg ttgccacggt tacgaaccgg gaacgagcgg atcgcgtctc
     3301 tggttacgga gcaaacgcat gtcttcggcc cccagtccgc agttgtaggg atctgcaggg
     3361 acagatctgc cgcttcctga tggtcggatt gattctacga acgagacttc cggcgtcgcc
     3421 cgggcgtaaa cacccgggct gaatcaaata agcccgctga agcaggctgg atggtgcgcg
     3481 ctggaaccat gcgcccgcac cgcgctacat ttttcgttcc ccaagccctc caccctcaac
     3541 cctcgcccct acggcaggat gtagcgccag gcgtcggtgg cgaagcgttc gcggacgcgg
     3601 gtttcggcgc cggcgcgctc gtcgtcggtc agggtgtcct ggcggagatc ggtcaggtcg
     3661 gcgaattcgg cgatgaggtg ctcgatgatc gtctcgcggg gcaggttcgt ctgctgccgg
     3721 agcggcccga cgcgcttgtc ggccgagcga atccccttgt cgctcagctt ttcctggcca
     3781 atccggagca cctccatcat gaccgggatg ttcatctggt aggccatcgt ggtgtggtgg
     3841 aggatcgcgc cgccgcggcg ggcctgggcg gcaccgccga tcttgccatt ggctgaggtg
     3901 atgtcgttga ggggcgcgta ccaggcatcg atgcccaacg agcgcagcgc ggagatcacc
     3961 cacgagtcga agaaggcgta ggactcggcg aagctcatcc cctccgcgat ccgggccggc
     4021 gcgtagagcg agtaggtgat cgcgccctcc ggctcgatga acatcgcgcc gccgccgctg
     4081 atccggcgca cgatctcgac gccccgctcc tgcgcattct ccacctggat ctcgttccga
     4141 accgactgga accggccgag gacgatgcaa ctcgccgtcc agctccagaa gcggagggtc
     4201 ggggggcgct ctccggcccc gacgcggagg gtcagctcct catcgagacc gaggttcatg
     4261 gccggcgagg ccggctccgt cgggagcatc acccacggtt cggatcgcca gcggttcagc
     4321 tcactcatag gtgtccaggg aagcaagcaa cgcccgttcg attgccgccg ccagtgcctc
     4381 aggggaggag ccgagccatt cgacaccggg cggaatggcg gccgcgatcg tcgcggtgcg
     4441 ttccgcaatc ggcatgtcgg ccggcgaacc ctcgaccgac gcggtgatcg cggtcagcgc
     4501 ttcttccggg tagaggaaga agtcaccagt gacctcgacg ttgcgcagcc gctcgtcctc
     4561 gacgtcgaaa tcgacctgga cgagcttgcc gccgggagtt ttgaaggcgc cgtgcatgga
     4621 tgcgttcctg atcagccgcc cagttcgtcg cggacctgct cgtcgaactc gacatgctgc
     4681 tcgacgtcgt cggcgacgct gatctcctgg ccggtccagt cgatggcggt ccgctcccag
     4741 tccttgagcg tcaggttcat gagcagggag acgagctcgc cgcggcccgt gtcaaaccgg
     4801 tccatcagct cctcggaggt tccgtccagc gtcgccgccg atggcgcgtc cggtggcgcc
     4861 ttgagcaggg gcgtgttggt tcggatcgtc gtctggatgc ggtcgagcag cgcggcatcg
     4921 cgctcggtta tcagggccag cgtggttcgc tggccgtcat cagtggtggt ggcgaggccg
     4981 gcgccgcgca gggcggccgg ggcagagagg agctcgtcga tcagctcgcg aaaggcatcc
     5041 agcacggcga agctcagccg agctgcggca cggcggccgc atccacgaat tgccagatca
     5101 gcccgtaaat gtcgcggacc gtcagcgatc ccggctggtc ggcgattatc gccgtacccc
     5161 gcatcagggc atcaccccgc acctttatga acgtctcgtg gtcggctttc agccgga
//
LOCUS       DASSGX010000282         2142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_487237, whole genome shotgun sequence.
ACCESSION   DASSGX010000282 DASSGX010000000
VERSION     DASSGX010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487237"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="VFI12_04940"
     CDS             complement(<1..795)
                     /locus_tag="VFI12_04940"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7055787.1"
                     /translation="MKSDALHSFVADAMSGRLSRREMFARAAALGLSATAASSLINLA
                     AAQDATPSSLTGKLEVFSWWTSPGEHPALEALFAAYTKANPGVEIIDAAVAGGGGGPA
                     QAVLQTRLQGKNPPDSWQSHPGHELIDQYVDAGYCETVTDLYASEGLDKAVPQGLIDQ
                     CSKDGEQYLVPVGVHRGNGLWYNKDVVQKAGVTVGETMTQDEFMAALDTLKGAGVTGL
                     AVGDKDGFVDAQTFENHLLGALGPDKYVSLFSGESAWDDQEVKDVMT"
     gene            1067..1747
                     /locus_tag="VFI12_04945"
     CDS             1067..1747
                     /locus_tag="VFI12_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055788.1"
                     /translation="MWRLKYMIYGMIPSAVLGILLYFLAPQLADALRDAVRDSAASTV
                     RSTFQQEVPGHVKPGRLVITEDQLLTAVEDADARERSWNISGMAVVIGDGRVSFVEDD
                     GNRSTSDATIASAVPQVVDGRIVLTDRRGVLSIFKPARDAIADEIENQLEILFARSKV
                     TPVSVTANNGQLVIVTQAIGGTTSTGNSAPTPTTGAQPTVPAVTPTPESTRSGGLIGN
                     PFSKTPTP"
     gene            complement(1836..>2142)
                     /locus_tag="VFI12_04950"
     CDS             complement(1836..>2142)
                     /locus_tag="VFI12_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055789.1"
                     /translation="PNPERPWESSAAAITASGLFQLAELTTDAERSTRYRDYAVRIMA
                     TLLDPEFVADQTPGWEGILKHGTYHERLDLGVDESVMWGEYFFVEALDKLLGAPAVQ"
BASE COUNT          362 a          718 c          670 g          392 t
ORIGIN      
        1 ggtcatgaca tctttcacct cctgatcgtc ccaggccgac tccccgctga acagggaaac
       61 gtacttgtcc gggccgagcg cgccgagcaa gtggttctcg aaggtctgcg cgtcgacgaa
      121 accgtccttg tcgccaacgg ccagtccggt cacgccggcg cccttgagcg tgtcgagcgc
      181 ggccatgaac tcatcctgcg tcatggtctc gccgacagtg acaccagcct tctggacgac
      241 gtccttgttg taccagagtc cgttgcctcg gtggacgccg accggcacca gatactgctc
      301 gccgtccttc gagcactggt cgatcagccc ctgcggaacc gccttgtcga gcccttcgga
      361 cgcgtagaga tcggtcactg tctcgcaata gccggcgtcg acgtactggt cgatcagctc
      421 gtggccgggg tgagactgcc aggaatccgg cgggttcttg ccctggaggc gggtctggag
      481 gactgcctgg gccgggccac cgccgccgcc ggcaacggct gcatcgatga tctcgacgcc
      541 cggattggcc ttcgtgtacg cggcgaacaa cgcttcaagg gcgggatgct cacccggcga
      601 ggtccaccag gagaagactt cgagcttgcc ggtcaggctg cttggggttg catcctgggc
      661 ggccgccaga ttgatcaggc tgctcgccgc tgttgcgctg aggccaagcg ccgctgcccg
      721 cgcgaacatc tcgcggcggg aaaggcgccc gctcatcgca tcggccacaa acgagtgcaa
      781 cgcgtcagac ttcacggtgt ctcgcctcct gaattgtctg cttcgccggt cgacaccgac
      841 aggcggcgcg cgatgcttca cgaacaagac ccccggaagt cgcctcccgg acgccgtagt
      901 tgtgcgattg cgcgttcgcc ggagttatag cccgtgggta tgacctcgtc aacatgggag
      961 cgctcccgtg cgtgtttcgc cgtgatgcca catggctgct cgcagtaatc ggggctcgtt
     1021 atgcacaata gcggcagcac attcgttgtt tggaggtacg cgcgcgatgt ggcgactgaa
     1081 atacatgatc tacggcatga tcccgtccgc ggtgctcggg atcctgctct acttcctcgc
     1141 cccgcaattg gccgacgcgc tgcgcgatgc cgtccgggat tccgcggcca gcaccgtgcg
     1201 atcaacgttt cagcaggaag tgcccgggca cgtcaaacct ggccggcttg tcatcacgga
     1261 ggaccagctc ctcaccgcgg tggaagatgc cgacgcccgc gagcgaagct ggaatatcag
     1321 cggtatggcg gtcgtcattg gggacgggcg ggtcagcttt gtcgaggatg acggtaatcg
     1381 ctcgaccagc gatgcgacca tcgcgtcggc ggtgccccag gtcgtcgacg ggaggatcgt
     1441 gctgaccgac cgccgcggcg tgctcagcat cttcaagccg gcccgcgacg cgatcgccga
     1501 cgagatcgag aaccagctcg agatcctctt cgccaggagc aaggtcacac cggtctccgt
     1561 cacggccaac aacggtcaac tggtgatcgt cacccaggcg atcggcggca cgacaagtac
     1621 cggtaactca gctcccacgc caacgaccgg cgcccagcca accgttccgg ccgtgacacc
     1681 gacgcctgaa tcgactcgct caggcggcct gatcggcaat ccgttcagca agacgccgac
     1741 tccgtaggtc agtggtcagt ggtcagtggt cagtggtcag catagtcccg gccccctggc
     1801 ccgccgttgc tgctcgcctg ctcgcctgct cgccttcatt gcaccgccgg cgcgccgagg
     1861 agcttgtcga gcgcttcaac gaaaaagtac tcgccccaca tcacactctc gtccacgccg
     1921 agatcgagcc gctcgtggta cgtgccgtgc ttgaggatgc cctcccatcc gggcgtctgg
     1981 tccgcgacga attcgggatc aagcagtgtc gccatgatcc ggaccgcgta gtcgcggtag
     2041 cgcgtgcttc gctccgcatc cgtcgtcagc tcggccagtt ggaacaggcc cgaagccgta
     2101 atggctgccg ctgaactctc ccatggccgc tcgggattcg gc
//
LOCUS       DASSGX010000283         2874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_814722, whole genome shotgun sequence.
ACCESSION   DASSGX010000283 DASSGX010000000
VERSION     DASSGX010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814722"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..1075)
                     /locus_tag="VFI12_04955"
     CDS             complement(35..1075)
                     /locus_tag="VFI12_04955"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7055790.1"
                     /translation="MISRITLQAQRSAATSSVLAVIGACLAGAVLLLLTGHDPWTVYR
                     LIVERGLLDLDGLTESLKKMAPLLIITAGLLTCLRAGIWNIGIDGQVVIGAIACGVIA
                     GELAGDLPRSLLLIVGAVVGGVAGGLWALGPAVLTARFGLNEIITTIMMNYLAFNLVS
                     WLVKGPFKAPEIVTAQTKQIPIGDRLPDIPFTDIHIGLIIGLVVIVAVWLLFRETVPG
                     TMLDVLGRNRRAARHAGLPVTRLIVGAFLLSGATAGLAGAIDVLGIFGLFKATYQPGY
                     GFMAFALAYLARLRSLAVVPFAAFLSVLVIGGDAMSRRANVPTEFVSVLEGLMLLFFA
                     LAVWFEGRRGAA"
     gene            complement(1072..2607)
                     /locus_tag="VFI12_04960"
     CDS             complement(1072..2607)
                     /locus_tag="VFI12_04960"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7055791.1"
                     /translation="MAVIALSGIAKSYGEVQALQGVSLTLQRGEIHALLGENGAGKST
                     LLGILSGLVQPDGGTIALDGRAIRLNTPADAIAAGVATVYQHFTLVPPLTAAENLRLG
                     LSRAGRISGRALARQLTALGIAVPLDTPVELLPVGQRQLVEIAKALLRRPRVLLLDEP
                     TSVLAGAEIAHLLELIRTIAGRGTTVVLVTHKLDEALAVADRVTVLRRGRVTGEINLR
                     AATERRPSLTTRLIAMMFGGREPVPESIPHAAARPEDSRVVARLHEIAARDDRGAPAL
                     NGLSLDCHAGELLGIAGVEGNGQQELAEILSGERHIDSGSLEINGRPMTNAGTRALID
                     AGVGVTTADRIAVGCVPGTSLAINLALKRIGSPPFAEGIRLHKFEIRQFAQSLIGRFD
                     IRPGNPDQPILQLSGGNIQRALLARELAFRPDLLVCHQPTAGLDLLTAEQMLARIHEA
                     AGEGAAVMLISSDLDELLRHCDRIAILYRGAVAGILSRAGFDPDRIARLMVTGLDEQE
                     PAA"
BASE COUNT          481 a          966 c         1043 g          384 t
ORIGIN      
        1 gcggcgaaat cgacgaccga gacgccgttg atggtcatgc cgcgcccctc cgcccctcaa
       61 accagacggc cagcgcgaag aacaacagca tcagcccctc cagcaccgag acaaactcgg
      121 tcgggacgtt ggcgcgccgg ctcatcgcat ccccgccgat cacgaggacc gagaggaagg
      181 cggcgaaggg gaccactgcc agcgagcgca gccgggcgag gtaggcgagg gcgaaggcca
      241 tgaagccgta gccgggctga taggtcgcct tgaacaggcc gaagataccg agcacgtcga
      301 tcgcgccggc cagcccggcc gtcgccccgg agaggaggaa cgctccgaca atcaggcggg
      361 ttacgggcaa accggcatga cgcgcggccc ggcggttgcg gccgaggacg tcgagcatcg
      421 tcccgggcac ggtctcccgg aagagcagcc agacggccac gatcacgacc aacccgatga
      481 tcaggccgat gtggatgtcg gtgaagggga tatccggcag gcggtcgccg atcgggatct
      541 gcttcgtctg cgcggtcacg atctccggcg ccttgaacgg ccccttgacc agccagctga
      601 cgaggttgaa ggcgagatag ttcatcatga tcgtggtgat gatctcgttc aggccgaacc
      661 gcgcggtcag caccgccggc ccgagtgccc acagcccgcc cgccacacca cccaccaccg
      721 cgccgacgat gagcaggagc gagcggggca ggtccccggc cagctccccg gcgatcaccc
      781 cgcaggcgat cgcgccgatc acgacctgcc cgtcgatccc gatgttccag atcccggcgc
      841 ggaggcaggt cagcagcccg gccgtgatga tcaggagcgg agccatcttc ttgagcgatt
      901 cggtcaggcc gtcgagatcg agcaggcccc gctcgacgat cagccggtag acggtccacg
      961 ggtcgtgacc ggtcagcaga agcaggaccg ctccggccag gcaggcgccg atcacggcca
     1021 gcacggagct ggtcgcggcc gagcgctgtg cctggagggt gatccggctg atcacgccgc
     1081 cggctcctgc tcatcgaggc cggtcaccat cagccgcgcg atccggtccg gatcgaatcc
     1141 ggcgcgggac aagatacccg cgaccgcgcc gcggtagagg atcgcgatcc ggtcgcagtg
     1201 gcgaagcagc tcgtcgaggt cggaggagat cagcatcacc gccgcgccct cgccggccgc
     1261 ctcgtggatc cgggccagca tctgctccgc agtcaggagg tcgaggccgg cggtcggctg
     1321 gtggcagacg agcagatcgg gccggaaggc gagctcccgg gccagcagcg cgcgctggat
     1381 attgccgccg gagagctgga ggatcggctg gtccgggttg ccgggccgga tgtcgaagcg
     1441 gccgatcagg ctctgcgcga actggcggat ctcgaacttg tgcagccgaa tcccctcggc
     1501 gaaggggggc gagccgatcc gcttcagggc gaggttaatt gccaggcttg tgcccggcac
     1561 gcagccgacg gcgatccgat cggccgtggt gacgccgacc ccggcgtcga tcagggcgcg
     1621 ggttccggcg ttggtcatcg gccggccgtt gatctcgagg ctgccggagt cgatgtgccg
     1681 ctcgccggaa aggatttcgg ccagctcctg ctgcccgttg ccttcgacgc cggcgatgcc
     1741 gagcagctca ccggcgtggc agtcgagcga gagaccgttc agggccggcg cgccacggtc
     1801 atcgcgggcg gcgatctcgt gcaggcgggc gacgacgcga gagtcctcag gccgggcagc
     1861 ggcgtgcgga atcgattccg gaaccggctc ccggccgccg aacatcatcg cgatcaaccg
     1921 ggtcgtcagg ctggggcggc gctcggtcgc ggcccgcagg ttgatctcgc cggtgacccg
     1981 cccccgccgc aggaccgtga cccggtcggc gacggccagc gcctcgtcca gcttgtgcgt
     2041 gaccaggacg accgtcgtgc cgcggcccgc gatcgtgcgg atcagttcga gcaagtgcgc
     2101 gatttcggcc ccggccagga ccgaggtcgg ctcgtcgagc agcaggacgc gcggccggcg
     2161 gagaagggcc ttggcgatct cgaccaactg ccgctggccg accggaagca attcgaccgg
     2221 tgtgtccagc ggcaccgcta tcccaagcgc tgtgagttgc cgggcaagcg cgcggccgct
     2281 gatccgcccg gcgcgggaga gcccgagccg caggttctcg gccgcggtca gcggcgggac
     2341 gagggtgaag tgctggtaga cggtcgcaac tccggcggcg atagcgtcgg ctggggtgtt
     2401 taggcggatg gcgcggccgt cgagggcgat cgtgccgccg tcgggctgga cgaggccgga
     2461 gaggatgccg agcagggtcg atttgcccgc cccgttctcg ccgagcaggg catggatctc
     2521 accgcgctgc agcgtcagcg agacgccctg caacgcctgc acctcgccat acgatttggc
     2581 aatcccggac agcgcaatga cggccatgac gggatgatag gcgagcaggc gagcaggcga
     2641 gcaggcgagg aggcgcgaag aaggggcgcg gagacggtgt aggggctcgc gggtgcaaac
     2701 ctgccctctc cacccatccc gtctcctgga gccaatcccg ttgtaggggc gtgcgggggc
     2761 acgcccgcgt tgacggccaa cccgggtcct gtccggcacg gaccttcatc cccggccttc
     2821 cttgcccaac gggccggggg atgaagggcg gatttgcccc cgcaaatccc taca
//
LOCUS       DASSGX010000284         2134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1469139, whole genome shotgun sequence.
ACCESSION   DASSGX010000284 DASSGX010000000
VERSION     DASSGX010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1469139"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VFI12_04965"
     CDS             complement(<1..189)
                     /locus_tag="VFI12_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055792.1"
                     /translation="MMAGEDDLTRASSGSAGQSAPARISRRAVFGGAGAMAIAGIGAG
                     TRVLAHGGDDDDDDDHSGP"
     gene            312..1184
                     /locus_tag="VFI12_04970"
     CDS             312..1184
                     /locus_tag="VFI12_04970"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HET7055793.1"
                     /translation="MVLLENSLVTTDWLQQNLGAEDLSIIDIRGYVKSRDLGGGKQVA
                     DYIPATDEYLAAHIPGSVFVDWTVDITDPDDSVKTQIAPPERFRQAMEARGIGDDSGV
                     VIVDHSSGHFATRMWWALRYYGHDNVAILDGGFNKWQREGRPLTDTVPTPSPAAFTPM
                     LRPESRATWEDVAREIDSPETTIVDARDADTYTGRVWRGPRKGHIPSAINLSSKTLYT
                     PEGTWKSDEELAEIMSAAGVTPERKTVAYCNGGVTATAVLFALHRLGNHNFANYDGSW
                     NEWGERQDLPAETD"
     gene            complement(1199..1271)
                     /locus_tag="VFI12_04975"
     tRNA            complement(1199..1271)
                     /locus_tag="VFI12_04975"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1236..1238),aa:Phe,seq:gaa)
     gene            complement(1292..1367)
                     /locus_tag="VFI12_04980"
     tRNA            complement(1292..1367)
                     /locus_tag="VFI12_04980"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1332..1334),aa:Val,seq:tac)
     gene            complement(1380..1467)
                     /locus_tag="VFI12_04985"
     tRNA            complement(1380..1467)
                     /locus_tag="VFI12_04985"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1431..1433),aa:Leu,seq:gag)
     gene            1665..>2134
                     /locus_tag="VFI12_04990"
     CDS             1665..>2134
                     /locus_tag="VFI12_04990"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642298.1"
                     /note="Catalyzes the reversible oxidation of malate to
                     oxaloacetate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HET7055794.1"
                     /translation="MSSASSGRRKVTVIGAGMVGGTVAQMLAIRDYADVVLVDIVEGM
                     PQGKSLDLMQMGTLLGFDTRLSGTNTYEETADSDVIVITSGIARKPGMSRDDLLKTNM
                     GIVESVTTQVVAGSPNAVVIVVSNPLDAMCHVAFDAAGFPRERVIGMAGVLDSAR"
BASE COUNT          389 a          680 c          674 g          391 t
ORIGIN      
        1 cgggccggag tggtcatcgt catcatcgtc gtcgccgccg tgtgcgagga ctctggttcc
       61 cgcgccgatt ccggcaattg ccattgcgcc ggcgccgccg aacactgccc gccggctgat
      121 gcgggccgga gcagattggc cggcgctgcc cgacgaagcc cgggtcagat cgtcttcacc
      181 cgccatcatg ctcctcccac atctgccggt cccgcatcca tatcagatac tgtacgggtc
      241 agcatgcacg ccggttggtt tggcgctacc cagacgccga atcccggttt gacggagttt
      301 tgggagcgat catggttcta ctcgagaatt cgcttgtgac gaccgattgg ctgcagcaga
      361 acctcggtgc tgaggatctc agcatcatcg atatccgcgg ctatgtgaag tcgcgcgatc
      421 tcggcggcgg caaacaggtg gccgactaca tccccgcgac ggacgaatac ctggcggcgc
      481 acatccctgg ttcggtcttc gttgactgga ctgtcgacat taccgacccg gacgactccg
      541 tcaagaccca gatcgcgccg cccgagcgct tccggcaggc aatggaagca cgcggcatcg
      601 gcgacgacag cggcgtcgtg atcgtcgacc attccagcgg ccacttcgcc accaggatgt
      661 ggtgggccct gcgctactac ggccacgaca atgtcgcgat cctcgacggc gggttcaaca
      721 aatggcagcg cgagggtcgg ccgctcacag acacggtgcc gacaccgtcg ccggccgcct
      781 tcacgccgat gctgcggccg gaatcccgcg caacctggga ggatgtcgcc cgggagatcg
      841 actcgccgga gacgacgatt gtcgatgcgc gcgacgccga cacgtacacc ggtcgcgtct
      901 ggcgcggtcc ccggaagggc catattccga gcgcgatcaa cctctcatcg aaaacgctgt
      961 acacgccaga aggcacctgg aagtccgatg aggagctggc cgaaatcatg tcggcggccg
     1021 gagtcacgcc agagcggaag accgtcgcct attgcaatgg cggcgtgacg gcaacggccg
     1081 tgctttttgc gctgcaccgg cttggcaacc acaacttcgc gaactatgac ggctcgtgga
     1141 acgagtgggg ggaacggcag gatttgcctg cggaaaccga ttgatcccca gggccgggtg
     1201 ccaagggccg gactcgaacc ggcgaccgca cgattttcag tcgtgagctc taccaactga
     1261 gctaccttgg cgggtggctg tggggcaccg ctggtgggcg atagagggct cgaacctctg
     1321 acatccacgg tgtaaacgtg gcgctctacc agctgagcta atcgcccgcg gtttgattgt
     1381 ggtgcccagg aagggatttg aacccccacg ccctgtacgg gcactagccc ctcaagctag
     1441 cgcgtctgcc gattccgcca cctgggcaag tgaccacaaa ggccaacgga ttatgcgaac
     1501 tcgccgcctc cgtgtcaacc gggaccccag tattttaccc cgaatcggga cctcccggcc
     1561 aacctgacaa gccggtgtgg cagcatcgcg atgcgaaggc ccgcgtgcgg tctgcctata
     1621 ctaccggggt tgatttgctt gggttcatcg caggagttgg gctaatgtca tctgcttcgt
     1681 cgggtcgtcg caaggtcacc gtcatcggcg ccggcatggt cggcggcacg gtcgcccaga
     1741 tgttggcgat ccgggactac gcggacgtcg ttttggtgga catcgtcgag gggatgccgc
     1801 aaggcaagtc gctcgacctg atgcagatgg gcaccctcct cggcttcgac acgcggttga
     1861 gcggtactaa tacctatgag gagacggccg actccgacgt gatcgtgatc acctccggca
     1921 ttgctcgcaa accgggcatg agccgtgacg acttgctcaa gacaaacatg ggcatcgtcg
     1981 aatcagtcac cacgcaggtt gtagccggat ctccgaatgc tgtggttatc gtcgtctcca
     2041 atccgctcga cgcgatgtgc cacgttgcct tcgacgctgc gggcttcccg cgcgagcggg
     2101 tgatcggcat ggccggcgtg ctcgactcgg cccg
//
LOCUS       DASSGX010000285         2642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_487420, whole genome shotgun sequence.
ACCESSION   DASSGX010000285 DASSGX010000000
VERSION     DASSGX010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487420"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..1259
                     /locus_tag="VFI12_04995"
     CDS             132..1259
                     /locus_tag="VFI12_04995"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7055795.1"
                     /translation="MAEIRVDQLTKTFGSLRAVDDVSFTFPDGQVTCLLGPSGCGKTT
                     LMRIIAGLEQQTSGAVYFGDQNVSRLSPSKRNIGMVFQYPVVYRGISVYRNIELPLQS
                     LKLSKAERTRRVEEAIHLMGLTASASTEVGKLDNGTRQKVSVAREVARQPQFMLFDEP
                     ITNVDANAKLQLKRSLKELTRALRQTTVYVTHDQTEAMTLADQIALMRDGKIVQCAPP
                     RELYNHPADRFGGWFLGNPGMVFFHATIAGDAESRVLASPLFASPVAVVGAIPGMSEV
                     TIGIRPEHIRVSGEPLSDGVPALLERKTIQIGGQYLVSLAVAGLSEHGFKAKVAPSAG
                     ERLPASGPVFVELPLDRITLFDAHGDKIAATLQPAQVPVAR"
     gene            1259..2038
                     /locus_tag="VFI12_05000"
     CDS             1259..2038
                     /locus_tag="VFI12_05000"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HET7055796.1"
                     /translation="MTRPFLFGTNFKMNQTPAESAAFYERLAASVTMPSGVHLFVIPA
                     YTSLAAVAEVAGGEPHEIWVGAQNVHWAPEGAYTGEISVPMLLALGVDLVLLGHAERR
                     RYFHEADVELNRKMLAALAGGLRVLLAIGETAEERGFGVSGETVLRQLKIGLHGVQPD
                     QLDRLQIAYEPVWSIGADGTPALPEDVAPIAATIRAALGELFAAPGHQIPILYGGSVD
                     PANAGSFTALPDIDGLFVGRAAWTVDGFLATYRAGLAGKRR"
     gene            2175..>2642
                     /locus_tag="VFI12_05005"
     CDS             2175..>2642
                     /locus_tag="VFI12_05005"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET7055797.1"
                     /translation="MAEVVSLGIHILDVLGRPVTRIPPRQDVDLIEEIRLTVAGTAAG
                     TSVDLAKLGAGVLAMGAIGEDAAGNFIVDTMSRYGIDTRGLVRKSGVQTSATMLPIRP
                     NGERPALHVLGANAELTAADIDLDAIATARHLHFGGTYLMPKLDGAPTARILAF"
BASE COUNT          445 a          921 c          820 g          456 t
ORIGIN      
        1 gccgaatcat ctggcgctct tcgacgccgc gactggcgcg gccctcggcc acggcgccca
       61 cgctcaccat cccgactccc cgccgtcgac tctcgactct cgactctcga ctctcgactc
      121 gggtgtccac catggctgag atccgcgtcg accagctcac caaaaccttc ggttcgctcc
      181 gcgccgtcga cgatgtctcg ttcaccttcc ccgacggcca ggtgacctgc ctgttggggc
      241 catccggctg cggcaagacg acgctgatgc ggatcatcgc cggtctcgag cagcaaacat
      301 caggagcggt ctacttcggt gatcagaacg tctctcgcct gtcaccgagc aagcgcaaca
      361 ttgggatggt cttccagtat ccggtggtct accgcggtat cagcgtctac cggaatatcg
      421 agcttcctct gcaatcactg aagctgtcga aggccgagcg gacgcgccgc gtcgaggaag
      481 ccatccacct gatggggctc acggccagcg cgagcaccga ggtgggcaag ctggacaacg
      541 gcactcgcca gaaggtgtcg gtggcgcgtg aagtggcccg gcagccgcaa ttcatgctct
      601 tcgacgaacc gatcaccaac gtcgatgcca atgccaagct ccaattgaag cggtcgctga
      661 aggaactgac ccgcgccctg cgccagacca cggtctacgt cacgcatgac cagacggagg
      721 cgatgactct ggcagatcag atcgccctca tgcgcgacgg gaagatcgtc cagtgcgcac
      781 cgccgcgcga gctctacaac caccctgcgg atcgcttcgg cggttggttc ctcggcaatc
      841 ccggcatggt ctttttccac gccaccattg ccggtgacgc cgagtcgcgc gtgctggcgt
      901 cgccgctctt cgcctcgccc gtggctgtcg tcggcgccat accagggatg tccgaggtga
      961 cgatcgggat ccggccggag cacatccggg tgtcgggtga acccctgtca gacggtgtac
     1021 cggcattgct cgaacggaag acgatccaga tcggcggaca gtacctggtt tcgctcgccg
     1081 tcgccggcct cagcgaacac gggttcaagg caaaagtcgc cccgagcgcc ggggagcggc
     1141 tacccgcgtc aggtccggtt ttcgtcgagt tgccgctcga tcggatcacc ctgttcgatg
     1201 ctcatggcga caagatcgcg gccacgctcc agccggccca agtcccagtc gctcgctgat
     1261 gacgcggcca ttcctcttcg gcacgaattt caagatgaac cagacgccgg ccgagtcggc
     1321 ggcgttctac gagcggctcg ccgcgtccgt gacgatgccc tccggagtgc atctgttcgt
     1381 catcccggcg tacacctccc tcgcggctgt cgccgaggtt gccggtggcg aaccacatga
     1441 gatctgggtc ggcgcgcaga acgtccattg ggcgcccgaa ggcgcgtata ccggcgagat
     1501 ctccgtcccg atgctgctcg ccctcggcgt cgatctcgtg ctcctcgggc acgccgaacg
     1561 cagacgctac ttccacgagg cggacgtcga gctgaaccgg aagatgcttg ccgcgctcgc
     1621 cggtgggctg cgcgtcctcc tcgccatcgg cgaaaccgcg gaagagcgag ggttcggcgt
     1681 tagcggcgag accgttctgc gccagctcaa gatcggcctg cacggggtcc agcccgacca
     1741 gctcgaccgg ctccagatcg cctatgagcc ggtctggtcg attggggcgg acggcacgcc
     1801 cgccctccct gaggacgtcg ccccgatcgc cgccacgatc cgagccgcgc tgggcgagct
     1861 cttcgccgcg cccggccacc agattccgat cctctatggc ggcagcgtcg accccgccaa
     1921 cgctggctcc ttcaccgcgc tgccggatat cgacggcctc ttcgtcggcc gcgccgcctg
     1981 gaccgtcgat ggcttcctgg ccacgtaccg ggccggtttg gcaggcaagc gaaggtgaga
     2041 gggggaaagg ctgatggggt gttggggaat cgagaagtcg gcgaagacga tcacctgatc
     2101 gccgatcgcc gatcgccgat agccgatagc ccctaacacc ctatcacccc aacacccgtg
     2161 gaaaggattt gcgcatggcc gaggtcgtga gtctcggaat tcacatcctc gatgtgcttg
     2221 gtcgcccggt gacgcgcatt cctccccggc aggatgttga tctgatcgag gagattcggc
     2281 tgaccgtcgc cggcaccgcc gccggcacca gtgtcgatct tgccaagctg ggcgcgggag
     2341 tcctcgccat gggcgccatc ggcgaggacg cggccggcaa tttcatcgtc gataccatga
     2401 gccgctacgg catcgacacg cgcggcctcg tccgcaaatc gggtgtgcag acctcggcca
     2461 caatgctgcc catccggccg aatggcgagc gcccggcgct gcatgtcctc ggcgccaacg
     2521 ccgagctgac cgcagcagac atcgatctcg acgccattgc gacggcgcgg catctccatt
     2581 tcggcggcac ctacctgatg ccgaagctcg atggcgcgcc gacagcgcgc atcctcgcct
     2641 tc
//
LOCUS       DASSGX010000286         3213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1469355, whole genome shotgun sequence.
ACCESSION   DASSGX010000286 DASSGX010000000
VERSION     DASSGX010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1469355"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VFI12_05010"
     CDS             complement(<1..284)
                     /locus_tag="VFI12_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055798.1"
                     /translation="MPRRSPLNRIPPLWLAVAVALLLLAPSLPASAESPVRVELAASP
                     VAITPTPMTAASGTSVKLPPAPAIDEGSSGPNVRTRTDRQGSDQIPKAPEP"
     gene            483..2243
                     /locus_tag="VFI12_05015"
     CDS             483..2243
                     /locus_tag="VFI12_05015"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7055799.1"
                     /translation="MTAPTSERVRYDNPPFPPYPKTERQAELMLLADELAAIAAEQAD
                     AHDRDNTFPFDTFAALKDAGYLALTVPKEYGGGGVNPLELMLAQERLARGNGSVALGV
                     TMHLTVVAGLEGGRGFPKPLLERVYRDVVENGAMINSAASEPDLGSPSRGGMYATTAV
                     RDGDGWRINGRKNWTTLAPGLAYTVVLLSEIQENGTRIRGNFLVPMDTPGVQIDETWD
                     NLGMRATGSHDVVYDNVWVSDEYRLPPSKEAPNTKVSEWSLVGSSVYLGIASAARDWI
                     VEYAKNRKPAGMSAPIAELPTVQQKIARIEVLLLESRATLYHTIGTWLDFPDSRPEIG
                     WQFAAAKHAVTNNAVEITDLAMRIAGSAGQFRRYPLERYFRDVRSGLGNPPIDDIALS
                     IVARSAWDSPSDRRYAVGFAGTLKSYLERKSEAVKAARASASDNQAERMQRLTAQVTA
                     EGGSGIRRVRIRDFQIITDAGPAMAGYDLGPRSPEVLLGALGSCISHTVLIQAAVDGL
                     PLDSLVVDVSADIDSLAGNADVRTPLQNFAFEIQIESSAQQEKFRRIIELLPDICPVL
                     NVLHTPQTFETTIMFNGTRI"
     gene            2257..2754
                     /locus_tag="VFI12_05020"
     CDS             2257..2754
                     /locus_tag="VFI12_05020"
                     /inference="COORDINATES: protein motif:HMM:TIGR04029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain protein"
                     /protein_id="HET7055800.1"
                     /translation="MSDDVINQFAGIAPRSDLAQLREQRPETLGYAQGSFDALLEPDD
                     PGGVSRAEREAIALRVATLERFPDLAGFHRERLRALGVSDTEIDAIVLFPGAGVLSAR
                     LAAIVRHTDLLTIDSRRGSPEALADLKQAGLAAADIVTISQLIAYLSYQIRIIALLRA
                     MRGEG"
     gene            2751..>3213
                     /locus_tag="VFI12_05025"
     CDS             2751..>3213
                     /locus_tag="VFI12_05025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01926.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HET7055801.1"
                     /translation="MSPRRAEQFTIDQLDWDPWIETVTEIGLSDEELAEVYGQAPNAK
                     GSPYFATLALDLPALVQRTGLFNNTMRSPGGASNAERELAATTVSRENGCVYCASVHA
                     QRYVFYANRPEVMQRLLDTGVDAPQDDPREQAIVDFSVKLTRNPAHLAAADL"
BASE COUNT          559 a         1035 c         1075 g          544 t
ORIGIN      
        1 ggttccggag ccttggggat ctggtcgctg ccctggcggt cggtgcgggt ccggacattc
       61 gggccggatg aaccttcatc aatcgccggc gccggtggca gctttacgga tgtgccggag
      121 gctgcagtca tcggcgttgg ggtgatggcg accggcgagg cggcgagctc gacacggacg
      181 ggactctccg ctgaggccgg gagcgacggc gcaagcagga gcagcgcgac ggccacagcc
      241 agccagagcg gcggaattcg gttcagcggg ctccgtcgtg gcacgccaat ctcccgatct
      301 gggcgatgcg cggggcgcgt ggatcaatct ccgagagctt tcgatagtct cagcatacgg
      361 cctctttccc gatttggatg tagcgtgggc taggcctgac cggggtactc cgcgttttcg
      421 cccggccatc tacaatcaat gaatgagata gccgcgattc ccgccgtgcc cgtggagcat
      481 tgatgaccgc accgaccagc gaacgcgtcc gctacgacaa tccgcccttc ccgccctacc
      541 cgaagaccga gcgccaggcc gagctgatgc tgctggccga cgagctggcg gcgatcgcgg
      601 ccgagcaggc ggatgcgcac gatcgcgata acaccttccc gttcgacacc tttgccgctc
      661 tcaaggacgc cggatacctt gccctgaccg tgccgaagga atacggcgga ggcggggtca
      721 acccgctcga gctgatgctg gcccaggaac ggctggcgcg tgggaacggc tcggtcgcgc
      781 tcggcgtgac gatgcacctg acggtcgtgg ccggtctcga aggcgggcgg ggcttcccga
      841 agccgctgct ggagcgcgtc tatcgcgacg tcgtcgagaa cggcgcgatg atcaactccg
      901 cggccagcga gcctgacctc ggcagcccca gtcgcggcgg tatgtacgcg acgaccgccg
      961 tccgcgacgg tgacggctgg cgaatcaacg gccgcaagaa ctggaccacg ctcgccccgg
     1021 gtctggccta caccgtcgtc ctgctctccg agatccagga gaacggaacc aggattcgcg
     1081 gcaactttct ggtgccgatg gacaccccgg gagtccagat cgacgagacg tgggataacc
     1141 tcggcatgcg cgccacggga agccacgatg tcgtgtacga caacgtctgg gtatcggacg
     1201 agtaccgcct gccgccgagc aaggaagcgc cgaatacgaa ggtcagcgag tggagcctgg
     1261 ttggcagctc ggtgtacctc ggcatcgcca gtgccgctcg agactggatt gtcgagtatg
     1321 cgaagaaccg gaagccggcc ggcatgtccg cgccgattgc cgagttgccg acggtccagc
     1381 agaagatcgc caggattgag gtcctcctgt tggagtcgcg cgccacgctc taccacacga
     1441 tcggaacgtg gctcgacttt ccggactccc gtccggagat cggctggcag ttcgctgccg
     1501 ccaagcacgc ggtgacgaac aacgcggtcg agatcaccga tctcgcgatg cgaattgccg
     1561 gttcggccgg gcaattccgc cgctacccgc tcgagcgcta tttccgcgat gtccggtccg
     1621 ggctcggcaa cccgccgatc gacgatatcg ctctctcgat cgtggccagg tcggcctggg
     1681 actcaccaag cgataggagg tatgcagtgg gatttgcggg cacgttgaag tcatacctgg
     1741 agcgtaagtc ggaggcggtc aaggcggcgc gggccagcgc ctcggacaat caggccgagc
     1801 gcatgcaacg tctgaccgcc caggtcacgg ccgagggcgg gagcggtatc cgaagggtgc
     1861 ggatccgcga tttccagatc atcaccgacg ccggtccagc gatggcgggg tacgaccttg
     1921 gcccgcgctc cccggaggtg ctgctcggcg cgcttgggag ctgcatctcg cacacggtcc
     1981 tgattcaggc cgccgtcgac gggctgccgc tcgattcgct tgtggtcgat gtctcggctg
     2041 acatcgattc gctggccggc aatgctgatg tgcgcacgcc gctccagaac ttcgccttcg
     2101 agatccagat cgagtcgagc gcccagcagg agaaatttcg gcggatcatc gagctgctgc
     2161 ccgatatttg cccggtcctg aacgtgctgc acaccccgca gacattcgag acgacgatca
     2221 tgttcaacgg cacccggatc tgatcgcgag gcgacggtga gtgacgacgt gatcaaccag
     2281 ttcgccggga tcgcacccag aagtgacctc gcgcagttgc gggagcagcg accggagacg
     2341 cttggctatg cccagggcag cttcgatgcg ctcctcgagc cggatgatcc cggtggggtt
     2401 tcgcgcgccg agcgagaggc gatcgcattg cgagtggcga cgctcgagcg gttcccggat
     2461 ctcgccgggt tccaccgcga acggctgcgc gcgctcggtg tttcggatac ggagatcgat
     2521 gccatcgtgc tgttcccggg cgcaggtgtg ctttcggctc gacttgccgc gatcgtgagg
     2581 cacacggacc tgctgacgat cgactcccgc cgcggctctc ccgaggcgct tgccgacctg
     2641 aagcaggcag gcctcgccgc ggccgacatc gtcacgatca gccagttgat cgcctatctc
     2701 agctaccaga tccggataat cgccctgctg cgcgcgatgc gaggcgaggg atgagcccac
     2761 gacgcgccga gcaattcacg atcgaccagc tcgactggga tccgtggatt gagacggtga
     2821 ccgagatcgg actgtcggac gaggagctgg ccgaggtcta cggacaggcg ccgaacgcga
     2881 aggggagtcc atatttcgcg acgcttgcgc tcgacttgcc ggcgctcgtc cagcgaaccg
     2941 gcctcttcaa caacaccatg cgcagtcccg gcggcgcgtc gaacgccgag cgcgagctgg
     3001 ctgcgaccac cgtttcccgc gagaacggtt gcgtttattg cgcctcggtc catgcccagc
     3061 gctacgtgtt ttacgcgaat cgacccgaag tgatgcagcg gctgctggac acgggtgtcg
     3121 atgctccgca ggacgatccc cgcgagcagg cgattgtcga tttctcggtc aaactcacgc
     3181 ggaatcctgc ccacctggcc gccgccgacc tcc
//
LOCUS       DASSGX010000287         3621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1033132, whole genome shotgun sequence.
ACCESSION   DASSGX010000287 DASSGX010000000
VERSION     DASSGX010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1033132"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..749)
                     /locus_tag="VFI12_05030"
     CDS             complement(27..749)
                     /locus_tag="VFI12_05030"
                     /inference="COORDINATES: protein motif:HMM:NF020968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase family protein"
                     /protein_id="HET7055802.1"
                     /translation="MTRDDKPLGQKAYGHIGHLPGSRRGPGDHGLSEGQARILTDKAR
                     DRHDVVIVQEKLDGSCVSVAKINGELVALGRAGYRAISSPFAMHHMFHVWATDRWAIF
                     NELLGEGERVVGEWLAQAHGTRYDLTGREPWVAFDLMRGVQRVTVEELRSRTMPLDIR
                     TPATLGTGPTTIEAAMKALGPFGHYGALDPAEGAVWRVERKGVVDFLGKYVRPDKVDG
                     SYLDSVTGGEPVWNWKPEPKAA"
     gene            complement(1074..1715)
                     /locus_tag="VFI12_05035"
     CDS             complement(1074..1715)
                     /locus_tag="VFI12_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055803.1"
                     /translation="MLQEARTVAEFRDLRDFASSMREWARAHGLGIEAENEACAFVLR
                     AEREIGRELLRMAESGERAVYGRFIVDQPRDENGRVLKGAKSTLPTLRDLGISLTNAA
                     NWQRLAGIEEEHFERMIAQALAPDANGKLRRIAKKRFYPWPEDRRGPHHGSDYRKDDP
                     LTPLFHLRTYADQVLGSSLSRLSSEDLTEVGKVGVKLVKAARAELDRRQTRAA"
     gene            complement(2033..2302)
                     /locus_tag="VFI12_05040"
     CDS             complement(2033..2302)
                     /locus_tag="VFI12_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055804.1"
                     /translation="MSVRPFPAWRDAPKGDEDLTAALCRIADCTNRPAYFVRDREYVD
                     VVCGQHIAPFLAEVLGTMGLSVCVEMIWRTEKVADATSGSEPVKL"
     gene            complement(2345..2512)
                     /locus_tag="VFI12_05045"
     CDS             complement(2345..2512)
                     /locus_tag="VFI12_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055805.1"
                     /translation="MPSKKPQTPKRPADMTTDEAIAHLFHPKVVEHAKRHARAAEKPA
                     TKADPKSMDGS"
     gene            2506..3438
                     /locus_tag="VFI12_05050"
     CDS             2506..3438
                     /locus_tag="VFI12_05050"
                     /inference="COORDINATES: protein motif:HMM:NF033547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HET7055806.1"
                     /translation="MASRKPTFTQEMNLVQLIEDFGSEDKCRKYLEALRWPNGVRCIR
                     CQSPKISRIKTRDQFDCDACGYQFSVRAGTIFHDSHLPLWKWFLAVYVMGESKKGISA
                     NQLKRMLGVSYKTAWYLCHRIRAAMLEEWEREPLSGIVEADETYTGHGPIRFGRELPG
                     RAVGGNKTAIIGAVQREGKVRLKVSDRVDRKALHAFLKAVVSDEAEAIYTDDYTAYFG
                     IADENTRHETVNHSRKQWVQGDVHTQTMEDVWSLFKRSVIGSYHHLSVKHLQAYLDEL
                     AFRYNNRQNDYLFRDTLLALIGSGSMPYAELIAG"
BASE COUNT          632 a         1203 c         1127 g          659 t
ORIGIN      
        1 ggatcccgat ctcgtggtgt taggggttag gcagccttgg gttccggctt ccagttccag
       61 acgggttcgc cgccggtcac gctgtccagg tacgagccgt cgaccttatc gggccggacg
      121 tacttgccaa ggaagtcgac gacacccttg cgttcgacgc gccagaccgc gccctcggcg
      181 gggtccaatg cgccgtagtg accgaacggt ccgagcgcct tcatcgcggc ctcgattgtc
      241 gtcggtccag ttccgagcgt cgcgggcgtt cggatgtcga gcggcatcgt ccggctgcgg
      301 agttcctcga cggtcacgcg ctggacgcca cgcatgaggt cgaacgccac ccacggctcg
      361 cggccggtca ggtcatagcg ggtcccgtgg gcctgagcca gccattcccc gacgacgcgc
      421 tcaccctctc cgagcagttc gttgaagatc gcccagcgat ccgtcgccca gacgtggaac
      481 atgtggtgca tggcgaacgg tgacgagatc gcccggtagc ccgcccgccc gagcgcgact
      541 agttcgccgt tgatcttggc gaccgacacg cacgagccgt cgagcttctc ctgaacaatc
      601 accacgtcat ggcggtcgcg ggccttgtcg gtgaggatgc gcgcctgacc ttcgctcagc
      661 ccgtggtcgc ccggtccccg acgcgatccg ggaaggtggc cgatgtgccc gtacgccttc
      721 tggccgagcg gcttgtcgtc gcgcgtcacc tcgcacctcg cagttgtcga atcaccggac
      781 gatctgagcg ctcatccacg atcaccagcc tccattcctg atctcagcga acgtccacgc
      841 gcagggtcgt gcgtggggtg gtcggacttt ggatccgtga acctggcaac catgttggca
      901 accatcgagg gccacgatgc ggtccagacc ggagccgcaa gccaccgtca cccgttcacg
      961 tcaggcacgc caggagaggg gagtgccgaa ggtgggatta ggcaccatcc gtgcggcagg
     1021 aacggctggg gagggtcttt ggcaaccatg ttggcaacca tccatgccga acgtcatgcg
     1081 gccctcgttt ggcggcggtc tagctcggcg cgagccgcct tgacgagctt cacgccgacc
     1141 ttcccgactt cggtcagatc ttcacttgac aatcgagaga gcgatgaccc gagtacttga
     1201 tctgcatagg tccgcaagtg aaacaggggc gtgagcggat cgtccttccg gtagtcggat
     1261 ccgtggtgag ggccgcggcg gtcctcgggc cacgggtaga accgcttctt ggcgatccgc
     1321 cggagcttcc cgttggcatc gggcgcgagc gcctgcgcaa tcatccgctc aaaatgttcc
     1381 tcttctattc ctgcgagccg ctgccagttc gccgcgttcg tcaggctgat cccgagatcg
     1441 cgcagagtcg gcagtgtgct cttggcaccc ttgagcacgc ggccgttctc gtctcgcggt
     1501 tgatcgacga tgaaccgacc gtagaccgct cgctccccgg actcggccat gcgaagaagt
     1561 tcccggccga tctcccgctc cgctcgcaag acgaacgcgc acgcctcgtt ctcggcttcg
     1621 atcccgagcc catgcgcacg agcccactcc cgcatgctgg acgcgaagtc gcgcagatca
     1681 cgaaactcgg caaccgtgcg cgcttcttgg agcatggtct tagcttttga gagttgagcg
     1741 atctcggtca cttctcggga caccccccac gacggggcga accctgaaca agacgccgcc
     1801 agcaggctca caaagaaatg tccggcccgc gccgcgcgtc gtctggccca ggcggacgtt
     1861 caggttgaac cgcctcccct ggttgccgca gacccggcag tcgccaacct cgttcatcat
     1921 cggtacagcg ccgcccaaag cccagcgcgg caccgatagc acaggcgttg acgccgccag
     1981 cgtcgccgat gacagccgcg gatagcgcag aaccacaggc ggtttttcat gctcacaatt
     2041 tgaccggctc actaccactc gttgcgtcgg caaccttctc ggtccgccag atcatctcga
     2101 cgcagacgct cagtcccatc gtcccaagga cctcggccaa gaacggagcg atgtgctggc
     2161 cgcagaccac gtcgacatat tcacgatcgc ggacgaagta ggcgggacgg ttcgtgcagt
     2221 ctgcgatccg gcaaagagcg gccgtcaggt cctcatcgcc tttcggcgca tcccgccaag
     2281 ccgggaacgg ccggacgctc atcggcttaa tgggttgtat acttgacaca gggccgttca
     2341 cgatctacga tccatccatg gacttcggat cggccttcgt cgcaggcttc tcagccgccc
     2401 gagcgtgccg cttcgcgtgc tccaccacct tcggatggaa caggtgggcg atggcctcgt
     2461 cggtcgtcat gtctgccggt cgcttggggg tttgaggctt cttcgatggc atcacgcaag
     2521 cccaccttca cgcaggaaat gaacctcgtt cagttgatcg aggatttcgg ctccgaggac
     2581 aagtgccgga agtacctgga ggcgcttcgc tggccgaacg gtgtccgctg catccgctgc
     2641 cagtccccga agatcagccg gatcaagacc cgcgaccagt tcgactgcga cgcctgcggc
     2701 taccagttca gcgtccgggc cggaacgatc ttccacgact cgcaccttcc cctgtggaag
     2761 tggttcctcg cggtctacgt catgggcgag tccaagaagg ggatcagcgc caaccagttg
     2821 aagcggatgc tcggggtctc ctacaagacc gcgtggtacc tgtgccaccg catccgggcc
     2881 gcgatgctgg aggaatggga gcgcgagcct cttagcggca tcgtggaggc cgacgagacc
     2941 tacaccggcc acggcccgat ccgcttcggg cgcgagctgc ccggtcgggc ggtcggcggc
     3001 aacaagaccg cgatcatcgg agccgtccag cgggagggca aggtccgcct gaaggtctcg
     3061 gatcgggtcg atcgcaaggc gctccacgcc ttcctgaagg cggtcgtgtc ggacgaggcc
     3121 gaggccatct acaccgacga ctacaccgcc tacttcggca tcgcggacga gaacacccgg
     3181 cacgagaccg tcaaccactc gcgcaagcag tgggtccagg gcgacgtcca tacgcagacc
     3241 atggaagacg tctggagcct gttcaagcgg tcggtcatcg gctcgtatca ccacctgagc
     3301 gtcaaacacc ttcaggcgta cttggacgag ctggccttcc gctacaacaa ccggcagaac
     3361 gactacctgt ttcgggacac gcttctggcg ctgattgggt cggggtcgat gccctacgcg
     3421 gagctgatcg cgggctgaca gacagcaaag accgccgccg atttcggata ttagtccttg
     3481 accatggcgg cggtctttgt gcttggggcc gggtcgcttg gcggcgattc gggctcccgc
     3541 gcggtgtcgg aacgcagcta ctgtcccacg ctcccagcta cgcgtcaagc gtccgattcg
     3601 ggtagtttcg cacagtgaat c
//
LOCUS       DASSGX010000288         8364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_380443, whole genome shotgun sequence.
ACCESSION   DASSGX010000288 DASSGX010000000
VERSION     DASSGX010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8364
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380443"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VFI12_05055"
     CDS             <1..447
                     /locus_tag="VFI12_05055"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7055807.1"
                     /translation="TDAAVERGLHKLYPMLRDVPVTHRWGGPIDRSRSGTLIFGRLTG
                     SPNVCYGVGYSGSGVGQSVVGGKILASTALDRVDEYSTSRLNQGHVLLYPPDPIRFFG
                     GLGVRAVLTHREEGEEDGRAPHPVVKAISKLAYPTLPRGLDRSRTR"
     gene            complement(546..1769)
                     /locus_tag="VFI12_05060"
     CDS             complement(546..1769)
                     /locus_tag="VFI12_05060"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET7055808.1"
                     /translation="MKPHARVQDNIFTRRAILAGGLTFGLAGCGGGAETTPTPAIPEL
                     QPTNTPEPVPTQAPLGSPVAGYSQPGKWDGRTLNVAAWGGNIEDAQGTAFFNPFEEAT
                     GASVQIKKADLGRLKEQVDSEDVSWDVLTVPADTALALARTGYLEPIDFAVVDKTPLV
                     PEVVLQYAVGEGFYSTVIVYRADNPAPPRGWTDFWTFSERESPDDPIPPQDLRTLPRN
                     PVGTLEFALLADGVPVEQLYPLDLERAFASLDRIRDYVVAWYEDGKQPIELLVADQVA
                     MGSAWNVRAWQLGVTTEVGTQWNGGMLSADMWVVPKGAPNKDIAMDFINFSTRAIPSA
                     NFSRLVPYGPVNLEAPPLLDPERLKQMPTAPENFSSQFIQNWVWWADNLDAVTARFED
                     WLLSKPESSPEAQSH"
     gene            1918..3435
                     /locus_tag="VFI12_05065"
     CDS             1918..3435
                     /locus_tag="VFI12_05065"
                     /inference="COORDINATES: protein motif:HMM:NF019011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HET7055809.1"
                     /translation="MTIRIALGGIVHETNTFALTPTDLAEFEANGLYVGDELRRLTGT
                     NTVVGGAIEAIDADPELELIPTVFSHAIPGGTVSREAFEHIVGQITDGITAAKPDAVV
                     LDLHGALVVDGFPGGDGEVARRVRAIVGPGVPIVSPLDLHGNIDDVLVQNVDILLPYH
                     TYPHVDTAERGREAVELAVRMAKGEIRPTMRAVKLAISPPGPQQYSGFEPTVSLQKIA
                     RDWETKPGVIDTSILFAFPYSDVPHNGMAVVATTDNDPELAEQICREIGAEIVRRHRE
                     FWPEMMTVEEAVHAAMSEPKGPVVLADYGDNPGGGSSCDGTALLWGLLDLGAQGAVVG
                     HICDPDVARVAHEAGEGAEINVELGGKRDHWHGAPIPVTATVLRLSGGDYVYEGPMNR
                     GVHNSMGPTAVLACRGRHGNIVEVIVSSRRVQALDTAIFRSQGIEPTERKILVVKSSV
                     HFRGAFAPIASRIILVNTPGLLQLDVSAFPFRHITRPLWPHDQVEPRMIMLQPSA"
     gene            3484..3750
                     /locus_tag="VFI12_05070"
     CDS             3484..3750
                     /locus_tag="VFI12_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055810.1"
                     /translation="METYNIYMDEASADGLNGDTELDVTFRVTAASADTEDAEENAVI
                     VGLDLVDLLNLRDALQNEIDSFTLQALEATALLQSGELEDEDLP"
     gene            complement(3838..4578)
                     /locus_tag="VFI12_05075"
     CDS             complement(3838..4578)
                     /locus_tag="VFI12_05075"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7055811.1"
                     /translation="MQSSANEVWKNDWVVRRFLDGVRGGIPYANDQLEVMLRLLDAGD
                     RPIKRFLDLGSGSGLLAIAILTRHPEAKATLVDFSEPMMEAARDLLGEDTTLPSFVLA
                     DLNSPDWVNELQDEEPFDAIVSGFTIHHLPHDRKAALYRELLPLLAPGALFINVEHVA
                     SASPWLEALWNEAMIDSLTEYHERQRTGQSREMITREYGQRPDQDANVLASVETQLTW
                     LRDAGFVDVDCAFKYFELAVLSGRRPVE"
     gene            complement(4607..5233)
                     /locus_tag="VFI12_05080"
     CDS             complement(4607..5233)
                     /locus_tag="VFI12_05080"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HET7055812.1"
                     /translation="MDWLILPKGMLLGVSIAAPVGPIGVLTIRRTIGDGFRMGFATGL
                     GAATADTTYGLVAAFGLTGVMSVLTAQADLIRLVGGLMLGMIGLRGIWHGRAAVRADS
                     AEVRRTMTPAGSYAQTVGLTLTNPMTIVAFIGMFAGVGISSGEGTLISALALVVGVGL
                     GSVAWWLFLAFTVSRVRHRLPDGAMRLINIGSSTVIVAFGVAALIAAR"
     gene            5687..7114
                     /locus_tag="VFI12_05085"
     CDS             5687..7114
                     /locus_tag="VFI12_05085"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7055813.1"
                     /translation="MNDGNGHLAPGYDIGKVRDAIKDNVWLHYTNMKAVRESTEKPLV
                     LVKGEGSTVWDSDGNAYLDCLAGIYSVNAGYGQRAIADAMMAQLEQIPFINPFGFTSV
                     PTAVFADRLADLVPLGGDARIFFTSGGGEAVETALKIAKKYQTVKGFPHRYKTISRRT
                     AWHGTTFGALSVNGLTDARNGFGPLMPGARHAPMSHRYRCPYCQNDTACNMTCYDEVE
                     RLVEFEGSETVACIIMEPVQNAGGCIPPGSRDYFKKIRKLCDETGILMIMDEVICGFG
                     RLGELFASDYYGVMPDIITTAKGITSSYAPLGAAMARKEIANTIDEGSVLNHGITFGG
                     HAVSCAAGIANLDIMLNEDLPGKAKETGAYLRQQLEDRLGNHPNVGDIRGAGLFLGVE
                     MVKDRETKETLPYDADYLNGMTDEMRRLGLIIRNDSRGDPTTQLCPPLVITKEECDTV
                     VDVLEHCFNQLGKKLGTVGTTVAVS"
     gene            complement(7271..>8364)
                     /locus_tag="VFI12_05090"
     CDS             complement(7271..>8364)
                     /locus_tag="VFI12_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7055814.1"
                     /translation="LGGSNDEREQTLNQLLVEMDGFDTDVEVIVMAATNRPDVLDPAL
                     LRPGRFDRQVTVGLPDRKGREAILAIHTRGIPVAKEVDLPGIARATTGFSGADLSNLV
                     NESALMAARKNRKEILNSDFDEALDKILLGTTRTNLMSPEERKVVAYHEGGHALVAHL
                     TPGADPLRKVTIVPRGRALGVAIQTPEEDRYNVSRLYLEGRLSVALGGRAAEIATFDE
                     ITTGAESDLKQATQLARRMVGLWGMSEEVGPVYLGTGEEHVFLGREIVQDKAFSDATS
                     ERVDTAVRQIVEQALETAQTLIEANKPKLEKLVAALLEKETLDAVEVTDILGPRPQLS
                     PREAEIMVPPPVAEPPLIGEPISTANRPE"
BASE COUNT         1524 a         2766 c         2661 g         1413 t
ORIGIN      
        1 accgacgcgg cggtcgagcg gggcctgcac aagctctacc cgatgctgcg cgatgttcca
       61 gtgacgcatc gctggggcgg accgatcgat cgcagccggt cgggcacgtt gatcttcggc
      121 cggctgaccg gatcgccgaa cgtctgctat ggcgtcggct actcgggctc aggcgttggc
      181 cagagcgtgg tcggcgggaa gatcctcgcg tcgacggcac tcgatcgcgt cgacgagtac
      241 tcgaccagcc ggcttaacca gggccacgtc ctgctctacc cgcccgatcc gattcgcttc
      301 ttcggcggct tgggcgtccg cgcggtcctg acgcaccggg aagagggcga agaggacggc
      361 cgcgcgccac acccggtcgt caaggccatc tcgaaactcg cctacccgac gctcccacgg
      421 ggccttgacc gctcccgcac ccggtaaacc atcggcccaa atcgtagttc cgggcgaccc
      481 gcccggcctg tcgacaacgg aagccgggat catcgcccgg aaccccatca agatgcgtcg
      541 gtcaatcagt ggctctgggc ctcgggcgat gattccggct tgctgaggag ccagtcctcg
      601 aatcgcgccg tgaccgcgtc gagattgtcc gcccaccaca cccaattctg gatgaactgc
      661 gaggagaagt tctcaggcgc ggtcggcatc tgcttgagcc gctccggatc gagcagcggc
      721 ggcgcctcga gattgaccgg tccgtagggc accaggcgcg agaagttggc cgatggaatc
      781 gcgcgggtgg agaagttgat gaagtccatc gcaatgtctt tgttcggcgc gccttttggg
      841 accacccaca tgtcggccga gagcattccg ccgttccact gcgtgccaac ctcggtcgtg
      901 acgccgagtt gccacgcccg cacgttccag gcgctgccca tcgccacctg atcagccacc
      961 agcagctcga tcggctgctt cccgtcctcg taccaggcga cgacgtaatc gcgaatccgg
     1021 tccaggctcg cgaaagcacg ctcgagatcg agcggataga gctgttccac cggtactcca
     1081 tccgccagca atgcaaattc aagcgtgccg acagggttgc gcggcaacgt gcggaggtcc
     1141 tgcggcggga tcgggtcgtc cggcgattcc cgctccgaaa aggtccagaa atcggtccag
     1201 ccgcgcggcg gagccgggtt gtcggcgcgg tagacgatga cggtcgagta gaagccctcg
     1261 ccgaccgcgt attgcaggac gacctcgggc acgagcgggg tcttgtcgac cactgcgaag
     1321 tcgatcggct ccaggtagcc ggtgcgggca agagcgagtg cggtatcggc cggcacggtc
     1381 agaacatccc aggagacgtc ttcggaatcg acctgctctt tcagccgacc gagatccgcc
     1441 ttcttgatct ggactgaagc gccggttgcc tcctcgaatg gattgaagaa tgcggttccc
     1501 tgcgcatctt cgatgttgcc gccccacgcc gcgacgttga gggtccggcc atcccatttg
     1561 cccggctggc tatagccggc gacaggggag ccgagcggcg cctgggtcgg cacaggctcc
     1621 ggggtgttgg tcggttgcag ctcgggaatg gccggcgtcg gagtcgtctc ggcgccgccg
     1681 ccacatcccg ccaggccgaa cgtcagccct ccggcaagga tggcacgccg ggtgaagatg
     1741 ttgtcctgaa cgcgtgcgtg cggcttcacg attcccccgt ttcgccgcag tgtccggcga
     1801 tagctcattc agccgcacat tatcgcgcat ggctcgcccg atgatgaatc ggcaccgaat
     1861 cgtccacttt ccctgctagg atggcgcgat tgcgtcgcat gagctggcag ggagagcatg
     1921 accattcgta tcgcgctcgg gggaatcgtc cacgaaacca ataccttcgc gctgacgccg
     1981 accgacctcg cggagttcga ggcaaacggg ctctacgtcg gcgatgagct gcgccggttg
     2041 accgggacca acaccgttgt cggtggcgcg atcgaggcga tcgacgccga cccggagctt
     2101 gagctgatcc cgaccgtgtt cagccacgcc atccccggcg ggacggtcag tcgcgaggcg
     2161 ttcgagcata tcgtcgggca gatcaccgac ggcataaccg ccgccaagcc ggacgccgtc
     2221 gtcctcgacc tgcatggcgc gcttgtcgtc gacgggtttc cgggcggcga cggtgaggtg
     2281 gcgcgtcggg tgcgggcgat cgtcggcccg ggcgtgccga tcgtctcccc gctcgatctg
     2341 cacggcaaca tcgacgacgt gctggtccag aacgtcgaca tcctcctgcc gtaccacacc
     2401 tatccacacg tcgacaccgc cgaacgcggg cgggaagcgg tcgagctggc ggtcaggatg
     2461 gcgaagggcg aaatccggcc gacgatgcgt gccgtcaagc tcgccatctc gccgcccggg
     2521 ccgcagcagt acagtggttt tgaaccaacc gtctcgttgc agaagatcgc gcgggactgg
     2581 gaaacgaaac cgggcgttat cgacacgtcg atcctcttcg ccttccccta ctccgacgtc
     2641 ccgcacaacg gcatggccgt cgtcgccacg acggacaatg atcccgaact ggccgagcaa
     2701 atctgccggg agattggggc ggagatcgtc cgccggcatc gcgagttctg gccggagatg
     2761 atgacggtcg aggaggccgt ccacgccgcg atgtcggaac cgaagggacc ggtcgttctc
     2821 gcggattacg gagacaaccc gggcggcggc tcgtcctgcg atggcacagc gctcctctgg
     2881 ggcctgctcg acctcggcgc gcagggtgcg gtggttggcc atatttgcga tcccgacgtg
     2941 gcccgggtcg cgcacgaggc gggcgagggc gccgagatca acgtcgagct cgggggcaag
     3001 cgcgaccact ggcacggcgc gccgatcccg gtcaccgcca ccgtcctgcg tttaagtggc
     3061 ggcgattatg tctacgaagg accgatgaat cgcggcgtcc acaactccat ggggccgact
     3121 gccgtgctcg cctgccgagg gcggcatggg aacatcgttg aggtgattgt cagctcgcgg
     3181 cgggtacaag cgctcgacac cgccatcttc cggtcacaag ggatcgagcc gaccgagcgg
     3241 aaaatcctgg tcgtcaagtc gagtgtccac ttccggggcg ccttcgcgcc gatcgcgtcg
     3301 cggatcattc tcgtcaacac gccgggattg ctccaactcg acgtctctgc cttcccattc
     3361 cgccacatca cgcgcccact ttggccgcat gatcaagtcg aaccgcgtat gatcatgctg
     3421 caaccgtccg cgtagccggt gcgttgcacc gatacgtact cagggaccct ggaggagcac
     3481 cacgtggaga cctacaacat ttatatggat gaggcctcgg cggacggtct gaacggcgat
     3541 accgaactcg acgtcacctt ccgcgtcacg gcggccagtg ccgacacgga ggacgcggaa
     3601 gagaacgcgg tcattgtcgg gctcgatctg gtcgatctgc tgaacctgcg cgacgcgttg
     3661 caaaacgaga tcgacagctt cactctgcag gccctggagg cgaccgccct cctccaatcc
     3721 ggagagctcg aagacgagga tctcccgtag cgaaccctga tcaatggaac ctcgacagga
     3781 aacgccgggc aaacccgccc ggcgtttccg ttcgacgaaa tacggtggtt cgccggccta
     3841 ttcgaccggc cggcggccgg acagcactgc caattcgaag tacttgaagg cgcagtcgac
     3901 gtcgacgaaa ccggcgtcgc gcagccatgt aagctgagtc tcaaccgagg ccagcacgtt
     3961 tgcatcctga tccggccgtt ggccgtactc gcgcgtgatc atctcccggc tctggccggt
     4021 tcgctggcgc tcgtggtact cggtcaacga atcgatcatc gcctcgttcc agagcgcttc
     4081 cagccaggga gaggcggacg cgacatgctc aacgttgatg aagagcgcgc ccggcgccag
     4141 caacggcaag agctcgcggt agagcgccgc cttccggtcg tgcggcaagt gatggatcgt
     4201 gaagccggag acgatcgcgt cgaacggctc ctcgtcctgc aactcgttca cccaatccgg
     4261 gctattgagg tcggccagga cgaaggacgg cagcgtcgtg tcttcgccga gcagatcgcg
     4321 cgcggcttcc atcatcggtt ccgaaaagtc gaccagcgtc gctttcgctt cggggtggcg
     4381 ggtcaggatc gcgatcgcga gcaagccaga cccgctgccg aggtcgagaa accgcttgat
     4441 cggcctatct cccgcgtcga gcaaccggag catgacctca agttggtcgt tcgcgtacgg
     4501 aatgccgccc cggacgccat ccaggaatcg gcggaccacc caatcgttct tccagacctc
     4561 gttcgcgctc gactgcatcg cttccttcca tcacgcagcc cctagcttac cgagccgcga
     4621 tgagcgccgc cacgccgaag gccacgatca cggtactgga gccgatattg atgagtcgca
     4681 tcgccccgtc cggaagccgg tgacgaaccc gcgatacggt gaaggccagg aacagccacc
     4741 aggcgaccga tccgagaccg accccgacga ccagcgccag cgcgctgatc agtgtcccct
     4801 ccccggacga gatgccgacg cccgcgaaca tgccgatgaa ggccacgatc gtcatcggat
     4861 tcgtcagggt cagaccaacg gtctgtgcgt aggacccggc gggcgtcatg gtccgccgca
     4921 cctcggctga atcagcgcgg accgccgctc gcccatgcca gatgccgcgc agcccgatca
     4981 tgcccagcat tagtccgccg accagccgga tcaggtcggc ttgggcggtg aggacggaca
     5041 taactccggt cagcccgaat gcggccacga ggccgtacgt ggtatccgca gtggctgcgc
     5101 cgagcccggt tgcgaagccc atgcggaagc cgtcgccgat cgtccgtcga atagtcagta
     5161 cgccgatcgg gccgaccggc gccgcaatcg acacgccgag gagcatgccc tttggcaaaa
     5221 tgagccaatc catctggagt tcctggaatt gaaacccgtt accgaccgat gaagtccact
     5281 ggccaccgct gaatgaagcc attaatggtg gcgccgggtt gctggtcgtc caccaagatc
     5341 aggcagaggt catcgggggc agaaccgagc gagccggcac gtggcccacg aacaacgaac
     5401 atgaggttgt ccagtttgtc gcgcctcatg cgtcacgcca cccgatcgtc agtcgctgcg
     5461 aggtgtgacg ggatcataca cgggaacctg ggccctcacc ccgcgtcgac agccctcgcc
     5521 agactccggt atgctggcgc aacgaccaca ttgaatatcg atggacgaaa acggcatgcc
     5581 cctgcttctt cgcgattact ggatctcgag cggcgcatcg ccgccaggcc gcgtctaccg
     5641 cacccactga tccgtcacat tttggaatcg tagaaaggcc gcgcgtgtga acgacggtaa
     5701 cggtcacctg gctcccgggt acgacatcgg gaaggtccgg gacgccatca aggacaacgt
     5761 ctggctccat tacacgaaca tgaaggcggt tcgggaatcg accgaaaaac cgctcgtcct
     5821 ggtcaagggc gaaggcagca cggtctggga ctcggacggg aacgcctacc tcgactgtct
     5881 ggccgggatc tactccgtca acgccgggta cgggcaacgc gcaatcgccg acgcgatgat
     5941 ggcgcagctc gagcagattc cgttcatcaa cccgttcggc ttcacgagcg tgccgacggc
     6001 ggtctttgcc gaccggctgg ccgatttggt cccgctcggc ggcgatgccc ggatcttctt
     6061 cacgtccggt ggcggcgagg ccgtcgaaac agccctcaag atcgcgaaga aataccagac
     6121 ggtaaagggc ttcccgcacc ggtacaagac gatatcgcgc cgcaccgcct ggcacggcac
     6181 gactttcggc gcgctctcgg tcaatggcct gaccgacgcc cgcaacggat ttggcccgct
     6241 catgccgggc gcgcgccatg cgccgatgtc gcaccgctac cgctgcccct actgccagaa
     6301 cgacaccgcc tgcaacatga cctgttatga cgaagtcgag cgactggtcg agttcgaggg
     6361 gtcggagacg gtagcttgca tcatcatgga accggtccag aacgcgggtg ggtgcatccc
     6421 gccagggtct cgcgactact tcaagaagat ccgcaagctt tgcgatgaga cgggcatcct
     6481 gatgatcatg gatgaggtca tctgcgggtt cggccggctc ggggagctat tcgcctccga
     6541 ttactacgga gtcatgccgg acatcatcac gacggccaag ggcatcacat cgtcatacgc
     6601 gccgctcggc gcggcgatgg cccggaaaga gatcgcgaac acgattgacg agggctccgt
     6661 cctgaaccac ggcatcacct tcggcggcca cgcggtctcc tgtgccgccg gcatcgccaa
     6721 cctcgacatc atgctcaacg aggacctgcc ggggaaggcg aaggagaccg gcgcgtacct
     6781 ccggcagcaa ctcgaagatc ggctcggcaa ccacccgaac gtgggcgaca tccgcggcgc
     6841 gggcctcttc ctcggcgtcg agatggtcaa ggaccgcgag acgaaggaga cgctgcccta
     6901 cgacgccgac tacctgaacg ggatgaccga cgagatgcgg cggctcggat tgatcatccg
     6961 caacgacagc cggggcgacc cgacgacgca gctctgccct cccctcgtga ttacgaagga
     7021 ggagtgcgac acggtcgtcg atgtgctcga gcattgcttc aaccaactcg gaaagaagct
     7081 cggcaccgtc gggaccaccg ttgcggtgag ctgatcaaga cgtagccccg ggcggcctcg
     7141 ccaggttttc caaagcgaga ggccgggcga gccgcccgga gctacatcca gaattcccgt
     7201 actgacacgc tccgcagaga aatgaaaagt ccccgaagcc ggtcggcttc ggggactttg
     7261 ttgtcatgcg ctattccggg cggttagcgg ttgagatcgg ctcaccgatc agcggcggct
     7321 cggccaccgg cggcgggacc atgatctccg cctcgcgcgg gctcaactgc ggtcgcggac
     7381 cgaggatgtc agtcacctcc accgcgtcga gcgtctcctt ctcgaggagg gcggctacca
     7441 gcttctccag cttgggctta ttggcctcga tcagcgtctg tgccgtctcc agcgcctgct
     7501 cgacaatctg gcgcacggca gtgtcgacgc gctcggacgt ggcgtcagag aaggccttgt
     7561 cctgcacgat ctcgcggccc aggaagacgt gctcctcgcc ggttccgagg tagaccgggc
     7621 cgacctcttc actcatgccc cagagtccga ccatccgccg cgccagctga gtcgcctgct
     7681 tgaggtcgct ctccgcgccg gtcgtgatct cgtcgaaggt cgcaatctcc gctgcccggc
     7741 cgccaagggc gaccgagagg cggccctcga ggtacaggcg cgagacgtta tagcggtcct
     7801 cctccggtgt ctggatcgca acgcccagcg cccggccgcg cggcacgatc gtcaccttgc
     7861 ggagtggatc ggcgccgggg gtcaggtgcg cgaccagcgc gtgtccaccc tcgtggtacg
     7921 cgacgacttt gcgctcctcc gggctcatca ggttggtccg ggtcgtgcca agcagaatct
     7981 tgtcgagcgc ttcgtcgaag tcggagttga ggatctcctt ccggttcttg cgggctgcca
     8041 tcagagccga ttcattcaca aggttggaga ggtccgcgcc cgagaagccg gttgtcgccc
     8101 gcgcaatgcc cgggagatcg acttccttgg cgaccgggat gccacgcgta tggatcgcca
     8161 ggatcgcctc gcgtcccttc cggtccggca agccaaccgt cacctggcgg tcgaaccggc
     8221 ccggccgcaa gagcgcgggg tcgagcacgt ccggccggtt ggtggcagcc atgacgatca
     8281 cttcgacatc ggtgtcgaaa ccatccattt cgaccagcaa ctggttcagc gtctgctcgc
     8341 gctcgtcgtt cgatccgccg agtc
//
LOCUS       DASSGX010000289         2213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1142773, whole genome shotgun sequence.
ACCESSION   DASSGX010000289 DASSGX010000000
VERSION     DASSGX010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142773"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="VFI12_05095"
     CDS             complement(<1..939)
                     /locus_tag="VFI12_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055815.1"
                     /translation="MGTHQNSVTRKRHRLGAARWFAMIGLMLALIPAWTPAVRAADQA
                     TINITAVADDGTPLPFARFQVIDSNGTLVTTRETTPPNGTVSIDVDVTDPSLTYTVTM
                     ETPPACATKPADQDVGPFSDGDSVDLTFETSFESSCDLGSVSFYAYTCPAGLDLTVDD
                     YGQYRDNCLQADNGEAFTLTEKGNTHQTFTLTTGEYGIAGRAPIVGLVPGDFTASQDG
                     SDPATTLAYCLTWEGTPVEAPAPSDTSKMKLNKDGEIALTVNGGDRIACDFFTVSEPL
                     PGADSGNDGGNSSADQPPADNGGTVSEDGQNAPEGAI"
     gene            1329..>2213
                     /locus_tag="VFI12_05100"
     CDS             1329..>2213
                     /locus_tag="VFI12_05100"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET7055816.1"
                     /translation="MTNPDRDAVDRESTPAADQAAGDGLTSHPVKRKPAIRNDARTAS
                     ESSAEVDAGADLAPRKVKPRRLDAPILTDLTGKQQIQGKHSGDRYVRVVRPQGEDFER
                     AGPGHLVATPEASEPSGPIRRGYMKVKRILIGQPLTTASAAHERLTKVKALAVLSSDA
                     LSSVAYATEEILRVLLVAGGLAILDVSLPISAAIILLLVIVGISYRQTIKAYPHGGGS
                     YIVAKDNLGDGPALVAGASLLAAYTLTVAVSIAASVAALISAFPALGTHRVALGIAFI
                     VLVTILNLRGIRESGSIFA"
BASE COUNT          363 a          735 c          722 g          393 t
ORIGIN      
        1 gatcgcgccc tcgggagcgt tctggccatc ctcggaaacg gttccgccgt tgtcggccgg
       61 tggctggtcg gcgctggagt tgccgccgtc gttgccggaa tcagcaccgg gcagtggctc
      121 ggagacggta aagaaatcgc aggcgatgcg atccccaccg ttaacggtga gcgcaatctc
      181 gccgtccttg ttgagtttca tcttcgacgt atcggacggc gccggcgcct caaccggcgt
      241 cccttcccag gtcaggcagt aggccagcgt ggtcgccggg tccgatccat cctggctcgc
      301 ggtgaaatca cccggcacga ggccgacgat cggggcgcgg ccggcaatgc cgtactcgcc
      361 ggtggtcagg gtgaaggtct ggtgtgtgtt gcccttctcg gtcagggtaa aggcctcgcc
      421 gttgtcggcc tggaggcagt tgtcgcggta ctggccatag tcatcaacag tcaggtcgag
      481 gccggcgggg caggtgtagg catagaagga gacactgccg aggtcgcagc tgctttcgaa
      541 agaggtctcg aaggtgagat caactgaatc gccgtccgaa aacgggccga cgtcctgatc
      601 ggccggcttg gtcgcacagg cgggcggcgt ctccatcgtg accgtgtacg tcagcgacgg
      661 gtccgtgacg tcgacgtcga tcgagacggt gccattcggc ggcgtggtct cacgagtggt
      721 gacgagtgtg ccgttactgt cgatgacctg gaagcgggcg aacggcagcg gcgtgccgtc
      781 gtcggcgacc gccgtgatat tgatcgtcgc ctggtcagcc gcgcgcaccg ccggcgtcca
      841 ggccgggatc agcgcgagca tgaggccgat catcgcgaac cagcgcgccg cgccgagtcg
      901 atgccgcttc cgtgtaaccg agttctggtg cgtacccatt tcctgccttc ccatgacagc
      961 gcgccacgcg ctccccctgt catgcacccg tttctggcac tcaaggacga ttcttggcct
     1021 cgtccccggg ccgtcgcgat cccctatacg tccccaacac tgccaccgac ggtcacgcgc
     1081 gctgggccat acaccccaca taacgcaccg gcagggaatg ggtttcgggg gcgcacacag
     1141 attctatgcg aaattgggtg gcgttgcccg gcgaggctgc tacaatccac cggccatgag
     1201 gaatcaatgc ctcgcactcc gagccatgcc gccagatgcc ggcccgattc ggaaacgtag
     1261 cggcgtggac agtggcgcca gtttgggccg tgcacaatgt cggcctgacg ggagcgagaa
     1321 gtcgcacgat gacgaatcct gatcgcgatg cggtcgaccg cgagtccaca cctgccgcgg
     1381 atcaagcggc gggcgatggg ctcaccagcc atcctgtcaa gcgcaaaccg gcaattcgca
     1441 acgatgcgcg aaccgcgagc gaatcatcgg ccgaggtcga tgccggtgcg gatctggccc
     1501 ctcgtaaggt gaaaccgcgc cggctcgacg ctccgattct gactgacctc accggcaagc
     1561 agcaaattca gggcaagcac tccggcgacc gctatgtccg cgtcgtccgg ccgcagggcg
     1621 aggacttcga gcgggccgga ccgggtcacc tggtcgccac gccggaggcc tccgagccga
     1681 gcggcccgat caggcgcggc tatatgaagg tcaagcgcat cctgatcggg cagccgctga
     1741 ccaccgcctc ggccgcgcat gagcgattga ccaaggtcaa ggcgctggcc gtgctttcgt
     1801 ccgacgccct ctcctccgtg gcgtatgcga cggaggagat cctgcgcgtc ctgctcgtcg
     1861 ccggcggctt ggcgattctc gatgtcagcc tgccgatctc ggcggcgatc atcctgcttc
     1921 tggtcatcgt cggcatctcc tatcgccaga ccatcaaggc ctacccccat ggcgggggca
     1981 gctatatcgt cgccaaggac aacctcggcg acgggccggc cctcgtcgcc ggcgcgtccc
     2041 tgctcgcggc gtacaccctg accgtcgcag tctcgatcgc cgcctcagtc gcggccctga
     2101 tctctgcctt cccggcgctg ggaacgcacc gtgtcgcgct cggcattgcc ttcatcgtgc
     2161 tggtcacgat tctgaacctg cgtggcatcc gcgaatcggg cagcatcttt gcc
//
LOCUS       DASSGX010000290         2662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_925902, whole genome shotgun sequence.
ACCESSION   DASSGX010000290 DASSGX010000000
VERSION     DASSGX010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_925902"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VFI12_05105"
     CDS             complement(<1..624)
                     /locus_tag="VFI12_05105"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7055817.1"
                     /translation="MTSSVHGFREGTAAMISGAGAFGNNQFDAIVIGSGAAGLTCATR
                     LAQAGQRVLLAEKNDWLGGYSHGFTQDGFYWDHGGHIFLAYKLGAQARQVFQRLKLDQ
                     RLEMVPDKHDYRCIFPDESLAIPADMTEAADVFAQRFPDEREGIARVLLIMESMIDQV
                     DTFVPSFRVADRPGERHLFDPVMEQFQRPKFGSLLSRNVRGQLMPGSN"
     gene            839..1396
                     /locus_tag="VFI12_05110"
     CDS             839..1396
                     /locus_tag="VFI12_05110"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HET7055818.1"
                     /translation="MTDWGKVEETNLRAVTDVLTSWNRHDVNGVLDFYDDGIIWTNVA
                     LEEQYHGKQEVGGFIRQLITAFPDLHFSVSERIPHEQYVAEKWEIHGTHLGPFLNIPA
                     TGRPIVIPGMSMVEMRNGKFYRDSFYFDSMGVLRQMGLMPSLTATQTLPGRALLWAAV
                     NRLPVATLAAFIAIVAFFARHSRRG"
     gene            1479..2585
                     /locus_tag="VFI12_05115"
     CDS             1479..2585
                     /locus_tag="VFI12_05115"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HET7055819.1"
                     /translation="MNDRSNSQAGGNSSAASSDHLFDVVAAFSAVPGPSGHEAPVRAF
                     LRERWQSHMAEWQEDRVGNILCRVGGSGPKLLIQAHMDEIGFVVRAITDDGFLLLDNA
                     QGTRRMSPERRQMIGQLAQVLGRHGVVAEGVFATASGHVLTQGQLDKPHLDFTDFFID
                     VGAKNRHEVDARGIHVGSPVIWNWPCRRFGTRIAGKAMDDRMLLAVMDLLLDDLDTDA
                     LRYDLWFGATIQEENGLHGARAMAHSTNFDAMIALDVGLVGDIPAISETEFVAKLGDG
                     PTLVHKDRDVHYDQPLIWRLADVAETHSIPFQHGVYTNYCSDGQPFIDHGTPAVLIGV
                     PTRYTHTAFEMVDESDITATVALLRAFATTPPDA"
BASE COUNT          534 a          817 c          829 g          482 t
ORIGIN      
        1 gttcgaaccg ggcatgagct gcccacgcac gttacgcgac aggagcgagc caaactttgg
       61 ccgctgaaac tgctccataa ccggatcgaa gagatggcgc tcgcccggtc gatcggcgac
      121 acggaaagag ggcacgaagg tatcgacctg gtcgatcatg ctctccatga tgagcaagac
      181 cctggcaatc ccctcgcgct cgtcaggaaa gcgctgggcg aacacatcgg ccgcttccgt
      241 catatcggcc gggatcgcga gcgactcgtc ggggaagatg cagcgatagt cgtgtttatc
      301 cggcaccatt tcgaggcgtt ggtcaagctt gagccgctgg aagacctggc gcgcctgggc
      361 accgagctta taggcaagga agatatggcc gccgtgatcc cagtagaagc catcctgggt
      421 aaagccgtgc gagtaaccgc cgagccaatc gttcttttca gccagcagca cccgttgacc
      481 cgcctgggcc aatcgagtgg cgcaggtcaa cccggcggca cccgagccga tgacgatggc
      541 atcaaattgg ttattcccaa acgctccagc acccgatatc atcgcagcag tcccctcacg
      601 gaagccatgc accgaggatg tcaacagaat tcaggttcgt tcgagcgagt tccgcttacg
      661 aaagtcacgc gaccgctggg gccacgtacc aggattcggt tcgattgtga agcccaaagg
      721 attcggacgt aagaatgcgc tcactatatg ggtgcggacg ccgggtgtca aggaaagatt
      781 gagcagcgga acggcccggc ggcatcgccc ggccgagcac gcgggaaagg attgaccgat
      841 gactgactgg ggcaaggttg aagagacaaa tctgcgcgcg gtgacggacg tgttgacgtc
      901 ctggaaccgc catgatgtta acggagtgct cgacttttat gatgatggaa tcatctggac
      961 gaacgtcgca cttgaagagc agtaccatgg caagcaggag gtcggcggct ttatccgaca
     1021 actcatcacc gccttccccg atttacattt ctcggtttcc gagcggatcc cgcacgagca
     1081 gtatgtcgcc gagaaatggg agattcacgg cacacatctc gggccgttcc tgaacatccc
     1141 ggcgaccggc cggccgatcg tcattcccgg catgagcatg gtcgaaatgc gtaacggcaa
     1201 gttctatcgc gatagcttct acttcgattc gatgggcgtc ctgcggcaga tgggcctgat
     1261 gccatcgctc acagccaccc aaaccctgcc cggccgcgct ctcctctggg ccgccgtcaa
     1321 ccgcctgccg gtcgccacgc tcgccgcgtt catcgcgatc gtcgcgttct tcgcccgcca
     1381 ttcgcggaga ggctaaccag cggaaatcat gcgatcatgg cgagcaacgc gccggcgggg
     1441 accggggcga cgattggtgt gggaaagggc gaacctccat gaacgatcga tccaactcgc
     1501 aggccggcgg caattcgagc gccgcaagct ccgaccatct cttcgatgtc gttgcggcgt
     1561 tttcggccgt gcccggccct tccgggcacg aagcgccggt gcgtgcgttt ctgcgcgagc
     1621 gctggcagtc gcatatggcg gagtggcagg aagatcgggt cggcaacatt ctctgccgtg
     1681 tcggaggaag cggtccgaag ctgctgattc aggcgcatat ggatgagatc ggctttgtcg
     1741 tgcgcgccat tactgacgac ggcttcctgc tgctcgacaa cgcccagggc acgcgccgga
     1801 tgtcgccgga gcgacgccag atgatcggcc agctggccca ggtactcggc cgacacgggg
     1861 tggtggcgga gggcgtcttc gcgacggcca gtggacacgt gctgacgcag gggcagctcg
     1921 ataaaccgca tctcgatttc accgatttct tcattgatgt cggcgccaag aatcggcacg
     1981 aagtggacgc gcgcggcata catgtgggat caccggtcat ctggaattgg ccctgccgcc
     2041 gcttcggtac gcgcatcgcg ggcaaggcga tggacgatcg catgctcctc gccgtcatgg
     2101 acctgctgct cgatgacctg gataccgacg ccctgcgtta cgacctctgg ttcggcgcga
     2161 cgattcagga ggagaatggg ctccacggcg ctcgggcgat ggcgcatagc accaacttcg
     2221 atgccatgat cgcgctcgac gtcgggctgg taggcgatat cccagcgatc tctgagacgg
     2281 agttcgtcgc gaagctcggt gacgggccga cgctcgtgca taaagaccgc gacgtgcatt
     2341 acgatcagcc gctgatctgg cgcctggccg atgtcgccga aacgcacagc atcccgttcc
     2401 agcacggcgt ctacacgaac tactgctccg acggtcagcc attcatcgac catggcacac
     2461 cggccgtgct gatcggcgtc ccgacgcgct acacgcacac cgccttcgag atggtcgacg
     2521 aaagcgacat cacggccacc gtcgcgttgc tgcgcgcctt cgcgacaact ccgccagacg
     2581 cgtgacacgc ggtgaacgcg gagattacga tctccggatc tcgacgctgg ctgagctggg
     2641 agcgagtaca cactatgctg ct
//
LOCUS       DASSGX010000291         2633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1249841, whole genome shotgun sequence.
ACCESSION   DASSGX010000291 DASSGX010000000
VERSION     DASSGX010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249841"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /locus_tag="VFI12_05120"
     CDS             <1..874
                     /locus_tag="VFI12_05120"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HET7055820.1"
                     /translation="CGHDGHVAMLLGTARMLIDRKDQFAGSVKLFFQPAEEGGGGALR
                     MINDGALDDLKPDAMFGIHLWNTRPVGTVNARVGAQLVGGDGFTITIHGKGGHGALPN
                     ICIDPVVAAAGVITALQTVISRTNDPLNPGVLTVGTIHGGEASNVIPDTVTMGGTIRF
                     VGQEQRMLMRKRLTEIAETTAAAYGARAEIDIEWGVMSTDNDPAMAALVQECAAQVVG
                     RENVSEGALLMVSEDMSEFLSRIPGCFFLVGSMNEERGLNWGHHTSRFDIDEESLGIG
                     IETMTRTVLTYLSR"
     gene            complement(894..1592)
                     /locus_tag="VFI12_05125"
     CDS             complement(894..1592)
                     /locus_tag="VFI12_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055821.1"
                     /translation="MSERPGIDQLLTEQPETSDALRVQILATEHWNLLMTRSMTWNEM
                     FTRASMYLTVVSAATVALALMAQATDFNDTFRLFALLVLPVVLFIGLATHIRLGDARG
                     EDVWFVIGMNRLRHGYLEIAPELAPYFVSSQYDDMAGIAQTYGSQGPPRPSRILASTP
                     VFVGVINAVIAGVLVGLIIEVLGGSIALAVVIGLIAGVTILVSLAGIRPYREISALQR
                     EYRPVFPQSESGEP"
     gene            complement(1714..>2633)
                     /locus_tag="VFI12_05130"
     CDS             complement(1714..>2633)
                     /locus_tag="VFI12_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055822.1"
                     /translation="LFGLLAMLGAVLLAQVGSVAVQGMDVSFSDVKLRELGYPEIVVT
                     VGTDGVDAPSELPAGYYLITLQPTEQFSTYLDIMLPPAGLSTDEATKLALDAAANDLA
                     QPDWGYLGGTNTFEVGVPVSFALYLAPGDYVWAASYYPMAQGGEETMKLVPLTVTGEA
                     TPAASPEASPAAGGPEAAVRLEMTDELVYVVSPDPLPAGDQIWEVSNTGETQAHHLVM
                     QRVPEGTTADQILDEFHGMMAGTPPAQDSISAQSTYVGYAALQSGRYTTHQEFDLDPG
                     SYAVICFIIDPATQMPHMMNGMVTVFTVK"
BASE COUNT          482 a          868 c          859 g          424 t
ORIGIN      
        1 ctgcggtcac gacggccacg tcgcgatgct gcttggcacg gccaggatgc tgatcgatcg
       61 caaggaccag ttcgccggct cggtcaagct cttcttccag ccggccgagg agggcggggg
      121 cggcgcgctg cggatgatca acgacggcgc gctcgacgac ttgaagcccg atgcgatgtt
      181 cggcatccac ctctggaaca cccgcccggt cggcacggtc aacgcccgcg tcggcgcgca
      241 attggtcggc ggcgacggct tcacgatcac gatccatggc aagggcggtc acggcgcgct
      301 gcccaacatc tgcatcgacc cggtcgtggc ggccgcgggc gtgatcaccg ccctgcagac
      361 ggtcatctcc cggaccaacg acccgctcaa tcccggcgtc ctgaccgtcg gcacgatcca
      421 cggcggcgag gccagcaacg tcatcccgga cacggtcacg atgggcggca ccatccgctt
      481 cgttggccag gagcagcgga tgctgatgcg gaagcggctc accgagatcg ccgagacgac
      541 cgccgccgcc tacggtgcca gggccgagat cgacatcgag tggggcgtca tgtcgaccga
      601 caacgacccg gcgatggccg ccctcgtcca ggagtgtgcg gcgcaggtgg tcggtcgcga
      661 aaacgtttcc gaaggagccc tgctgatggt gtccgaggac atgagcgagt tcctctcccg
      721 catccccggc tgcttctttc tggtcggcag catgaacgag gagcgcggct tgaattgggg
      781 ccaccacacc agccgcttcg acatcgatga agaatccctc ggcatcggca tcgaaaccat
      841 gacccgcacg gtgctcacgt acctctcgag gtaaggcgag caggggcgtt gtcttacggt
      901 tcgcctgatt ccgattgcgg gaataccggc cgatattccc gttgcagcgc cgagatctcg
      961 cggtatggcc gaatgccggc caatgagacc aggatggtga cgccggcaat gagcccgatc
     1021 acgaccgcca gcgcaatgga gccgccaagc acctcgatga tcaacccaac cagaacgccg
     1081 gcgatcaccg cattgatcac gccgacgaac accggcgtgc tcgcaaggat gcgactggga
     1141 cggggcggcc cctgggagcc gtaggtctgc gcgatcccgg ccatgtcgtc gtactggcta
     1201 ctgacgaagt agggcgccag ctccggcgcg atctcgaggt agccgtggcg cagccggttc
     1261 atcccgatca cgaaccagac gtcctcaccc cgcgcgtccc cgagacggat atgcgtcgcg
     1321 agcccgatga agaggacgac cggaaggacc agcagagcga ataggcggaa cgtgtcgttg
     1381 aagtcggtcg cctgcgccat caaagcgagc gcgacggtcg ctgctgagac gacggtcaga
     1441 tacatgctgg cccgggtgaa catctcgttc caggtcatgc ttcgcgtcat cagcagattc
     1501 cagtgctcgg ttgccaggat ctggacgcgg agggcatcag acgtttcggg ttgctccgtc
     1561 agaagttgat caatcccggg acgttcgctc atcggggtca cctcacgccg tgtggaggtc
     1621 gaattgcacg tgaatccatc atacggcgga cgcagaaacc cccgcgcctg ccgtttgacc
     1681 ggcgggagcg ggggttggag gcgacgggtg ctgctacttg accgtgaaga ccgtcaccat
     1741 gccgttcatc atgtgcggca tctgggtggc agggtcgatg atgaagcaga tcaccgcgta
     1801 gctgcccggg tcgaggtcga actcctggtg ggtcgtgtat cggcccgact gcagcgccgc
     1861 atacccgacg taggtcgact gcgccgaaat cgaatcctgc gccggcggcg tgccggccat
     1921 catcccgtgg aactcgtcca gaatctggtc ggccgtcgtg ccctccggga cgcgctgcat
     1981 gacgaggtgg tgcgcctgcg tttcgccggt gttgctcacc tcccagatct ggtcgccggc
     2041 cgggagcgga tcgggcgaga cgacatagac cagttcatcg gtcatttcga ggcggactgc
     2101 cgcctccggg ccgccggcgg ccggtgatgc ctcggggctg gcggccggcg tggcctcgcc
     2161 ggtcaccgtt aacggcacga gcttcatggt ctcttcgccg ccctgcgcca tcgggtaata
     2221 ggacgccgcc cagacgtagt cgcccggagc caggtagagc gcgaaggaga ccggcacgcc
     2281 cacctcgaac gtgttggtcc cgcccaggta gccccagtcc ggctgcgcga gatcattggc
     2341 cgcggcatcg agtgcgagct tcgtcgcctc atcagtcgag agaccggccg gcggcagcat
     2401 gatgtcgaga taggtcgaaa actgctccgt cggctgcaac gtgatcaggt aatacccggc
     2461 cggaagctcc gacggcgcat cgacaccatc cgtgccgacc gtgacaacga tctcggggta
     2521 ccccagctcc cgcagcttga catcgctgaa cgacacgtcc atgccctgca ccgcgaccga
     2581 cccaacctgg gccagcagaa ccgcgccaag catcgccaat aacccaaaca agc
//
LOCUS       DASSGX010000292         2661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_380988, whole genome shotgun sequence.
ACCESSION   DASSGX010000292 DASSGX010000000
VERSION     DASSGX010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380988"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..472
                     /locus_tag="VFI12_05135"
     CDS             149..472
                     /locus_tag="VFI12_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055823.1"
                     /translation="MVCAADGSAYVCSATAGAPQQEICNGVDDDCDGVIDNGAVCLPV
                     PNAISICSGGQCQYACKQGFADCDGDPVNGCEQMVSSDPLNCGGCGVICSGATPTCVN
                     GTCVA"
     gene            834..2486
                     /locus_tag="VFI12_05140"
     CDS             834..2486
                     /locus_tag="VFI12_05140"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7055824.1"
                     /translation="MASGQYNRKTGLGGAQIDRRRLLQGAAALGIGASTGVVSVQAAR
                     AQNDAATLTIAIQGSPSDLDPHSQYDYRSVIPIRGAYEGLIVLKDSETDQYEGQIAES
                     WEANEDKSVWTFHLRDGVTFQDGSPCDAEAVRASYERLLTMQKGAYNVVARFVPDPSH
                     ITAPDAKTVVFDLGKPQPLFEAAMGGTYGTQVVNVKVAMEHEEDGDFGNTWMMTYAEG
                     TGTGPYKITGFEPGDSVTLEKYDGYWAGWDGNHFDTIVVRSVNESQTIRQLLESGEAD
                     ISDRIALLPETVKELESNPKLKVDRHTTTEVVYMTMTEYGPLESAEARQAMCYAFPYA
                     EVIQGVYAGYAKQPRGAVAAATRGFDADTFQYATDTAKAKELLGTAGVAEGTKLSLMV
                     AGTTTVAELFQANLAEIGITLDIQAVDSNLFTGTFYGDSPVEERPNFMIWSWWPDYND
                     AWNHLDPQVSCDPHGSANAGMYCNERVDELLKASHDAANPEDYQTAMDEIQQILSKDD
                     PSAIYYAEPEWTTVMQADIQGFFLNPINLGTYNFYKLSRGTA"
BASE COUNT          495 a          809 c          926 g          431 t
ORIGIN      
        1 caattgcggc acgtgcggga cggcctgcat atcgggcgtg tgcacggccg gtgtttgcca
       61 ggcgccgccc agcacattct gccagcccgg ccagagtacg ggtcagccct gttcagccgg
      121 catcggagct tgccaccgcg agggcacgat ggtttgcgcg gcggatggga gcgcatacgt
      181 ctgcagcgcg acggccgggg cgccgcagca ggagatctgc aacggggtcg atgacgactg
      241 cgatggcgtg atcgacaacg gggcggtctg cctaccggta ccgaacgcga tcagcatctg
      301 ctctggcgga cagtgccagt acgcgtgcaa gcagggcttc gccgattgcg atggcgatcc
      361 cgtcaatgga tgcgagcaaa tggtctcgtc cgatccgctc aattgcggtg ggtgcggcgt
      421 gatctgctcg ggtgcgacac cgacctgtgt gaacgggacc tgcgtggcgt aaccgccgag
      481 gacaatcgat cgccaggatg gccaccgcga caggaaaggg gttgcggttg gccatcctgg
      541 agtcgattcg gtgtcgggat tggttcccgc caacatccgg gtcgggacga cgtccgggcc
      601 ggccacgggg gaccggtgct acatcgtgga acggacgcac cgatcatcgc ccggcggttg
      661 atcaacgggc acatcccctc tccaccacct ctcccctcca ggggagaggg catcatccgc
      721 gccacggtac gggcctccga ttgttcgggc gcgtctcctc gatttggttg gtaagatgcg
      781 gcgtcgcgat ggtgtgcgat gcggcacgat gacgagtatc aggaggtgcg gggatggcat
      841 cagggcagta caacaggaag accggcctcg gcggagcgca gatcgaccga aggcgcttgt
      901 tgcagggcgc ggcggccctc gggatcgggg cgagtacggg cgtggtctcg gtccaggcgg
      961 cccgggccca gaatgacgcg gcgacgctga cgatcgcgat ccagggttcg ccatccgacc
     1021 tcgatcccca ttcgcaatac gactaccgct cggtgatccc catccgcggc gcctacgagg
     1081 ggttgatcgt cctcaaggac agcgagacgg accagtacga gggccagatc gctgaatcct
     1141 gggaagcgaa cgaggacaag agcgtctgga ccttccacct gcgcgacggc gtcaccttcc
     1201 aggacggttc gccgtgcgat gcggaggcgg tccgggcctc ctatgagcgg ttgctgacga
     1261 tgcagaaggg cgcctacaac gtggtcgccc gcttcgtgcc cgatccctcg cacatcacgg
     1321 cgccggacgc gaagacggtc gtcttcgacc tcggcaagcc gcagccgctg ttcgaggcgg
     1381 cgatgggcgg tacctatggc acgcaggtcg tcaacgtcaa ggtcgccatg gagcatgagg
     1441 aagacggcga cttcggcaac acctggatga tgacctacgc cgagggcacc ggcaccgggc
     1501 catacaagat caccgggttt gagccgggcg attcggtcac cctcgagaag tacgacggct
     1561 actgggccgg ctgggacggc aaccacttcg atacgatcgt ggtccgctcg gtcaacgagt
     1621 cgcagacgat ccggcaattg ctcgaatcgg gcgaggcaga tatcagcgac cggatcgcgc
     1681 tgctgccgga gacggtcaaa gagctggagt cgaacccgaa gctcaaggtc gaccgccaca
     1741 cgacgaccga ggtcgtctac atgacgatga ccgagtacgg gccgctcgaa tcggctgagg
     1801 ctcggcaggc gatgtgctac gccttcccgt atgccgaggt gatccagggc gtctatgcgg
     1861 ggtatgccaa gcagccgcga ggcgcggtgg ccgcggcgac gcgcgggttc gacgcggaca
     1921 cattccagta cgcgaccgac accgccaagg cgaaggagct gctcgggacg gccggcgtcg
     1981 ccgagggcac gaagctcagc ctgatggtgg ccgggacgac gacggtggcg gagctgttcc
     2041 aggcaaatct ggccgagatc gggattacgc tggacatcca ggccgtggac tcgaacctct
     2101 tcaccgggac cttctacggc gactccccgg tcgaagagcg accgaacttc atgatctgga
     2161 gctggtggcc ggattacaac gacgcctgga accacctcga tccgcaggtt tcgtgcgatc
     2221 cgcacggctc ggccaacgcc gggatgtact gcaacgagcg ggtcgatgag ctgctcaagg
     2281 cctcgcacga cgcggcgaac ccggaggatt accagaccgc gatggacgag atccagcaga
     2341 tcctctcgaa ggatgatccg tcggcgattt actacgccga gccggagtgg acgacggtga
     2401 tgcaggcgga tatccagggc ttcttcctga acccaatcaa cctgggcacg tacaacttct
     2461 acaagctgtc gcggggcacg gcgtagtcgg cagacgttgg acgccagaca tcagttgaca
     2521 gaggacaggg gtcaggtggc gggtgcgctg cctggccccc gctttgtacg ggcgcgttga
     2581 tggtggttgc cattttgagt cggtaccggt gggtacgaac cgtaccgtag cgcggggtta
     2641 ccatgccccg ctgtggccgc a
//
LOCUS       DASSGX010000293         2259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_54131, whole genome shotgun sequence.
ACCESSION   DASSGX010000293 DASSGX010000000
VERSION     DASSGX010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54131"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1254)
                     /locus_tag="VFI12_05145"
     CDS             complement(<1..1254)
                     /locus_tag="VFI12_05145"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HET7055825.1"
                     /translation="MIWAMIQQVGGQVATMIVFFVLAALLPPGDFGLIGMAGAWLAIL
                     NAFCESGLGAALIQREQLREDHLTSTFGINVAVGTALTVLGVALSWPAAAYFGTPALQ
                     PVMALLSLGFLVRAFGLTQAALAQRELRFRALAVRDLTANIVGGSIGVALAVAGYGVW
                     SLVVMTLVSALLQTVLLWRLTHWRPRRFEISRRAAVELWPYSSRMLTFNLFKALAQNT
                     DRLIIGPVLGVRALGLYTLAFRIVIYPVTTFVGALGAYLFPKVARLQADSGAVRAVYR
                     AVLVGTLNLVLPALVAVAVLAPTIMPILGPRWHDAIPIVQILTIAALGQALMALVGQI
                     MKGLGRPGWLVWWSVGLTVLTAIALWIGARWGLSGVTLAYALAHLAALPVIFMIGRRL
                     TGLGMGEVLEIIWRPAVAGGILAVAL"
     gene            complement(1251..>2259)
                     /locus_tag="VFI12_05150"
     CDS             complement(1251..>2259)
                     /locus_tag="VFI12_05150"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET7055826.1"
                     /translation="LARTTVNGRGRPALAAARMLRPGAWFRTMTRVSLAARIIEAHDI
                     RVISAYNLLLGAPVGAIAAEMYGLPLVVTNLGEIYSHRAAIERQLPLVRHVTTVATVL
                     TSLTRHCAESYRQVGITRDVRVLHYGIDRERFALASGGDPVRARFGIPPSADVVLYVG
                     RLVRDMGLHVLIDGIPALLSRRPATHVLIAGGAGELDGAAKAAMARWPHRVAVAVDVP
                     ESELPGYYAAATLVVAPTVGARACGSLASAEAMAAGKPVVATRIGGIPEYVEDGVTGL
                     LVAPADPGALVEAVLTLLEDRQRLADFGRRGRARVGELFDGECTNADLEALFREVAGL
                     R"
BASE COUNT          404 a          803 c          766 g          286 t
ORIGIN      
        1 cagcgcgacc gcgagtatcc cgccggcgac cgccggccgc cagatgatct cgagcacctc
       61 ccccatcccg aggccggtca ggcgccggcc gatcatgaat atcacgggca gtgcggcaag
      121 atgcgccagg gcgtaggcga gggtgacgcc ggagagtccc cagcgcgcac cgatccacag
      181 tgcgatggcc gtcagcaccg tcaggcccac cgaccaccag acgagccagc cggggcggcc
      241 gagtcccttc atgatctgcc ccaccagggc catgagcgcc tgcccgagcg cggcaatcgt
      301 gaggatctgc acgatcggaa tcgcatcgtg ccagcgcggg cccagaatcg gcatgatcgt
      361 cggcgccaga acggcgaccg cgaccagcgc cgggagcacc aggttgaggg tgccgacgag
      421 caccgcgcga tagaccgcgc gaaccgcgcc tgaatctgcc tgcagtcgcg cgactttcgg
      481 gaacaggtaa gcacccagcg cgccgacgaa cgtcgtcacc gggtagatca ctattcgaaa
      541 cgcgagggtg tacaacccca gagcccggac cccgagcacc gggccgatga tcagccggtc
      601 ggtattctgg gcgagcgcct tgaacaggtt gaacgtgagc atccgcgaac tgtacggcca
      661 cagctcgacg gctgcccggc gcgaaatctc gaatcggcgg ggacgccaat gggtcaggcg
      721 ccacagcagc acggtctgga gcagagcgct caccagcgtc atcacgacga gactccatac
      781 cccgtagccg gccaccgcca gggcgactcc gatggaaccg ccgacgatgt tcgcggtcag
      841 gtcgcgcaca gccagcgcgc ggaaccggag ctcgcgctga gccagcgccg cctgcgtgag
      901 tccgaacgcg cgcaccagga atcccagaga gagcagcgcc atgacgggct gcagcgcggg
      961 agtcccgaag taggccgcgg cgggccagga gagcgcgacg cccaggacgg tcaacgcggt
     1021 tccgacggcg acgttgattc cgaacgtcga ggtcaggtga tcctctcgaa gctgctcccg
     1081 ctggatcagg gcggccccga gaccgctctc acagaaggcg ttcaggatgg ccagccaggc
     1141 gccggccatt ccgatgaggc cgaaatcgcc ggggggcagc agggccgcga gcacgaagaa
     1201 cacgatcatc gtggcgacct ggccgcccac ctgttggatc atcgcccaga tcatcggagc
     1261 cccgccactt cccggaagag cgcctcgagg tcggcgttgg tgcattcccc atcgaacagc
     1321 tcgcccacgc gcgcccttcc ccggcgaccg aagtcggcga ggcgctgccg atcctccagc
     1381 agcgtcagga ccgcttcgac gagggccccg ggatcggccg gagcaacgag cagtccggtc
     1441 acgccgtcct cgacgtactc gggaatccca ccgattcgcg tggccaccac cggtttcccc
     1501 gcggccatcg cctcggccga cgcgagactc ccacaggctc gtgcccccac tgtcggtgcc
     1561 acgaccaggg ttgccgcggc gtagtagccg ggcagctcgc tctccggcac gtccacggcc
     1621 accgcgacgc ggtgtggcca tcgagccatc gccgccttcg cggcgccgtc gagctccccg
     1681 gcgccgcccg cgatcaggac gtgggtggcc ggacggcggc tgagcagcgc cggaatgccg
     1741 tcgatcagga cgtgcagccc catgtctctc accagacggc cgacgtagag gaccacgtct
     1801 gccgacggag ggataccgaa acgggcgcgt accgggtcgc caccggaggc gagtgcgaac
     1861 cgttcgcgat cgatgccgta atggaggact cgcacgtcgc gcgtgatgcc cacctggcga
     1921 tagctctcgg cgcaatgccg ggtgagcgac gtcagcaccg tcgccaccgt cgtgacgtga
     1981 cggaccaggg gcagttgccg ctcgatcgcc gcgcggtggc tgtagatctc gcccaggttg
     2041 gtgacgacga gcgggagccc gtacatctcg gccgcgatcg caccgaccgg tgcgccgagc
     2101 aggaggttgt acgcgctgat cacgcggatg tcgtgggctt cgatgatccg cgcggccagg
     2161 ctcacgcggg tcatcgttct gaaccaggcc ccgggccgaa gcatccgcgc ggccgcgagc
     2221 gccggccggc cacgaccgtt caccgtcgtg cgcgcgaga
//
LOCUS       DASSGX010000294         2806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_161759, whole genome shotgun sequence.
ACCESSION   DASSGX010000294 DASSGX010000000
VERSION     DASSGX010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161759"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1206
                     /locus_tag="VFI12_05155"
     CDS             <1..1206
                     /locus_tag="VFI12_05155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HET7055827.1"
                     /translation="KLRHTPIFTFINKLDRPAKHPLELLDEIETTLRIKTIPMNWPIG
                     DGPEFRGVYDLRTQTVHLFERTEHGAKRAPVKVATLDDPSLDTLLGTQRAKELRDDVE
                     LINSAGHEFDLDAVLRGELTPVCFGSAITNFGVQLFLDTFVDYAPAPGAYATSAAVIE
                     PEQPEFTGFVFKIQANMDPRHRDRVAFLRICSGRYSRDMEVWHSRMKRKVRLAPPLRL
                     FGQEREVIEEAYAGDVIGVINPGLFAIGDTVSERDLGEFPPLPRFQPEHFALVRATRS
                     DKYKQFLKGLSQIEEEGAIQLFYPVASGRREPILAAVGALQFDVVRFRLESEYTVETT
                     LEPLSFSAVRWVAGDPQEITTVANSRGRLRAEDRDGNPVILFSTAWDLRYAEENAANL
                     TFSPTPITG"
     gene            complement(1315..2382)
                     /locus_tag="VFI12_05160"
     CDS             complement(1315..2382)
                     /locus_tag="VFI12_05160"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper oxidase"
                     /protein_id="HET7055828.1"
                     /translation="MSLVSNDVNRRRLLGMLGVAGAGATASAFAPQVPFTADKVSLAS
                     GSEHAGHDIVPVASNQKATMTADEMDAHHEAGIKMFPAKTEGKGGYILEPEMDGDIKV
                     FNLTCDVVQWEHSPGTIVEAWTYNGVVPGPEIRATEGDRVRINVTNNLPESTAIHYHG
                     LMVPNNQDGVPFITQPPIKPGQKYTYEFTIKPGNAGAHMYHSHHNSTEQVTKGLLGAF
                     VVEPKDTSTRPAYDVDYTMILGDGPIGGFHINGKGFPATEPIVVKKGQRLLIRFMNEG
                     LMIHPMHLHGFPMQVIAHDGWLVPQPYYCDTLNVAPGQRFEVIITPDEPGVWAFHCHI
                     LSHAESRDGMFGMVTALIVEE"
     gene            complement(2515..>2806)
                     /locus_tag="VFI12_05165"
     CDS             complement(2515..>2806)
                     /locus_tag="VFI12_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055829.1"
                     /translation="SIQGVIRDTESSGFTEEELEGTRQLMTGRLALTMQTNAGIASLL
                     QTIHEFDLGLDYVERYPNILAAVTLDSTRAALAGAIDPARLQVAVAGPAMAT"
BASE COUNT          503 a          907 c          881 g          515 t
ORIGIN      
        1 aagctgcggc acacgccgat cttcacgttc atcaacaagc tggaccggcc ggccaagcac
       61 ccgctcgagt tgctcgacga gatcgagacc acgctcagga tcaagacgat cccgatgaac
      121 tggccgatcg gcgacggtcc ggagtttcgc ggcgtctacg atctccgcac gcagacggtt
      181 cacctcttcg agcggaccga gcacggcgcg aagcgggcgc cagtcaaggt cgcgacgctc
      241 gatgacccct cgctcgacac gctgcttggc acacagcgcg caaaggagct tcgggatgac
      301 gtcgagctga tcaacagcgc cggtcacgag ttcgacctcg acgccgtcct gcgcggcgag
      361 ctgaccccgg tctgcttcgg gagcgcgatc acgaacttcg gcgtccagct cttcctcgac
      421 actttcgtcg actacgcgcc ggcgccgggc gcctatgcga cctcagccgc ggtcatcgag
      481 ccggagcagc ccgagttcac cgggttcgtc ttcaagatcc aggccaacat ggacccgcgc
      541 caccgggacc gcgtcgcctt cctgcgcatc tgcagcggcc ggtattcgcg cgacatggag
      601 gtctggcact cgcgaatgaa gcggaaagtc cgcctcgctc cgcccctccg cctgttcggg
      661 caagagcgcg aagtgatcga ggaggcctac gccggcgatg tgatcggcgt gatcaacccg
      721 ggcctgttcg cgatcgggga taccgtcagc gagcgggatc tcggcgagtt cccgccgctg
      781 ccgcgcttcc agccggagca tttcgcactg gtccgggcga cccggtccga caagtacaag
      841 cagttcctga aggggctgag ccagatcgag gaggagggcg cgatccagct cttttacccg
      901 gtcgccagcg gccggcggga accgatcctg gctgccgtcg gcgcccttca gttcgacgtc
      961 gttcgtttcc gcctggagtc ggagtacacg gtcgagacta ccctcgagcc gctgtcgttc
     1021 agcgcagtcc gctgggtggc gggcgatccg caagagataa cgaccgtcgc caatagccgg
     1081 ggccggttgc gcgcggaaga tcgggacggc aacccggtca tcctcttcag caccgcctgg
     1141 gatctccggt acgccgaaga gaatgctgcc aatctcacat tcagcccgac tcccattacc
     1201 gggtaagcac caatcaatgt agccccgggc ggccagcccg gcgtaatcac acgcgagacc
     1261 aaagagaacg agcgtcggga gtatcccgga cgcccgttca ttgcagtgac cggactactc
     1321 ttcgacaatg agcgccgtga ccatcccgaa catgccgtct ctcgactcgg cgtggctcag
     1381 aatgtggcaa tggaaggccc acacgcccgg ttcatccggc gtgatgatga cctcaaagcg
     1441 ctggccaggc gccacgttca gggtatcgca gtagtacggc tgcggcacga gccatccgtc
     1501 gtgggcgatg acctgcatcg ggaagccgtg caggtgcatc gggtggatca tcaacccctc
     1561 gttcatgaac cggatgagga gccgctggcc cttcttgacg acgatcggct cagtggccgg
     1621 gaaccccttg ccgttgatgt ggaagccgcc gatcggaccg tcgccgagaa tcatcgtgta
     1681 gtcgacgtca tacgccggcc gcgttgacgt gtcctttggc tcgactacga acgcgcccaa
     1741 caagcccttc gtcacctgct cagtcgagtt gtggtgcgag tggtacatgt gcgcgccggc
     1801 gttgccaggc ttgatcgtga actcgtaggt gtatttctga ccgggcttga tcggcggttg
     1861 tgtgatgaac ggcacgccgt cctggttgtt cggcaccatc aggccgtgat agtggatcgc
     1921 cgtgctctcc gggaggttgt tggtcacgtt gatgcggacg cgatcaccct cggtggcgcg
     1981 aatctccgga cccggcacga cgccgttata ggtccacgct tcgactattg tgcccggcga
     2041 gtgctcccac tggaccacgt cgcaggtcag attgaagacc ttgatatcgc cgtccatctc
     2101 cggctcgagg atgtagccac ccttgccctc ggtcttggcc gggaacatct tgataccggc
     2161 ctcgtgatgg gcatccatct cgtcggccgt catcgtggcc ttctggttgc tggcgacggg
     2221 gacaatgtcg tggccggcat gctccgaacc gctggccagg ctgaccttgt ccgcggtgaa
     2281 tggcacctgg ggcgcgaacg cgctcgccgt cgcgcccgcg cctgccacgc cgagcatgcc
     2341 gagcagccgt ctgcgattga catcatttga tacgagagac acagatgatc ccttctgcct
     2401 gaatccaacc gggcgcaaga acgctccggc accgctaccc aaaagggtgc ggtccgtagc
     2461 gcaaaaccag cgttgtcgat cgaaggggaa acgttacaag ttcgtaacag gcgtctacgt
     2521 tgccattgcg ggtccggcaa ctgccacttg tagccgtgca ggatcgatcg cgcccgcgag
     2581 ggcggcgcgc gtggaatcaa gcgtcacggc ggcgaggata ttcggatatc tctcgacata
     2641 gtcgaggccg agatcgaact catggatcgt ctggagcagg ctggcgatgc cggcgttggt
     2701 ctgcatggtc agggcgagcc gtccggtcat caactggcgg gtgccctcca gctcctcttc
     2761 agtgaagcca ctcgactcag tgtcccggat caccccctgg atactc
//
LOCUS       DASSGX010000295         2233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1361288, whole genome shotgun sequence.
ACCESSION   DASSGX010000295 DASSGX010000000
VERSION     DASSGX010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1361288"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..393
                     /locus_tag="VFI12_05170"
     CDS             <1..393
                     /locus_tag="VFI12_05170"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7055830.1"
                     /translation="TPQLLELAGPSARNVFFTTHEGIYGDQSDAAKKFNESYTTEYGK
                     GPDSIFAALGYDGINLLADAITRANDTAGDKIRDALAATQGFKGATGEISYSKDSHIP
                     VKSVAIVETVDGKFQLIKTTVPKVVPKA"
     gene            473..1822
                     /locus_tag="VFI12_05175"
     CDS             473..1822
                     /locus_tag="VFI12_05175"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HET7055831.1"
                     /translation="MGNQEPVTTSAIIERDNQYVADAIKIRYTPFVLAGGEGSRLFDA
                     EGNSFLDFSSLWSLANLGYSDRTVRQAVKDQVDRTMCSVLASAINEPGGLLAERLAGM
                     MSKQPKKVWFGLSGSDASEAAQRLLLKATGKRRLISFIGSWHGTTDATMALSGFAGFN
                     DRFGGSNIVKIPYPDPYRRPFPTHPDLSLTDQVLGYLEDVLFATICPPEDVAGIFVEG
                     IQADSGDIVPPDDFLPKLRALCDKHDIYLVMDEVKVGLGRTGKMFSYEHAGIEPDILL
                     LGKSLGAGLPLSAVIGPPEVLDCGTGLALYTISGNATSCAAALAGLDAIIRHGLVEQA
                     ASTGRYLLDRLTSLMRHDQVGDVRGRGMICGVDLVTDRDSKTPNSALAAKVVYRAWQL
                     GLIVFYAGTSSNVLEITPPLILTEEEVDEGVAILDQAIDDAVNGRVDEDAVAAYAGW"
     gene            1825..>2233
                     /locus_tag="VFI12_05180"
     CDS             1825..>2233
                     /locus_tag="VFI12_05180"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET7055832.1"
                     /translation="MTFDSRITTYLQIARDRYITELTRVCAIDSGSYHKPGIDQVQDH
                     FQRRMTELGFEVERVRNAEWGDDLVARRRGTGKGRILLIGHADTVYPVGEAEKRPVVI
                     EGDKLLGPGTCDMKAGILTGIYAVEALNAIGWTD"
BASE COUNT          411 a          733 c          712 g          377 t
ORIGIN      
        1 acgccgcagc tgctcgagtt ggccggaccg agcgcccgca atgtcttctt cacgacgcac
       61 gagggcatct acggcgacca gtccgatgcc gccaagaagt tcaacgagtc gtacaccacc
      121 gaatacggca aggggccgga ctcgatcttc gcggcgctcg gctacgacgg catcaacctg
      181 ctggccgacg cgatcacccg cgccaacgac accgccggcg acaagatccg cgacgcgctc
      241 gccgcgacgc aggggttcaa gggcgcaacc ggcgagatca gctactccaa ggactcgcac
      301 attccggtca agtcggtcgc gatcgtcgag accgtggatg gcaagttcca gttgatcaag
      361 accaccgtgc cgaaggtcgt gccgaaggcg taatccagtc atgtatgcga gcatgccctc
      421 gtgataagcg agggcatgtt tttctgcctg ccagcggttt ggaggcaaat tgatggggaa
      481 ccaggaaccg gtcacgacat cggccatcat cgagcgtgac aaccaatacg tcgccgacgc
      541 gatcaagatc cgctacacgc cgttcgtcct ggccggtggc gaggggtcgc gcctgttcga
      601 cgccgaaggc aattcgttcc tcgacttcag ctcgctctgg tcgctcgcca acctcggcta
      661 cagcgaccgc accgtgcggc aggcggtcaa ggatcaggtc gaccggacga tgtgctcggt
      721 cctcgcctcg gcgatcaacg agccgggcgg gttactggcc gagcggttgg ccgggatgat
      781 gtcgaagcag ccgaagaagg tctggttcgg cttgtcgggc tccgacgcca gcgaggcggc
      841 ccaacgcctc ctgctcaagg cgaccgggaa gcgacgactg atctccttca tcggcagttg
      901 gcacggcacg actgatgcga ccatggcgct ctccgggttc gccggcttca acgaccgctt
      961 cggcggctca aacatcgtca agattcccta cccggatccc taccgtcgcc cgtttccgac
     1021 gcacccggac ctctcgctga cggaccaggt gctcggctac ctcgaggatg tcctcttcgc
     1081 cacgatctgc ccgccggagg atgtcgccgg catcttcgtg gagggcatcc aggccgacag
     1141 tggcgatatc gtcccgccgg atgatttcct gccgaagctg cgcgcgcttt gcgacaagca
     1201 cgacatttac ctcgtgatgg acgaggtcaa ggttggtctc ggacggaccg gcaagatgtt
     1261 ctcctacgag catgccggga tcgagccgga catcctcttg ctgggcaagt cgcttggcgc
     1321 aggcctccca ctcagcgccg tgattggccc gcccgaggtg ctcgattgcg gcaccggact
     1381 cgccttgtac acgatctccg gcaacgccac gagttgcgcc gccgcgctcg ccggactgga
     1441 cgcaatcatc cggcacggac tggtcgagca ggcggcgtcg accggccgat acctgctcga
     1501 tcggctcacc agcctgatgc ggcacgacca ggtcggcgat gtgcggggcc ggggcatgat
     1561 ctgcggcgtc gaccttgtca ccgaccggga cagcaagacg ccgaattcgg cattggccgc
     1621 caaagtggtc taccgggcct ggcaactcgg cctgatcgtc ttctatgccg gcacctcctc
     1681 gaacgtgctc gagatcacgc cgccgctgat cctgaccgaa gaagaagtcg acgagggtgt
     1741 tgcgatcctc gaccaggcga tcgatgacgc ggtcaacggc cgggtcgatg aggacgcggt
     1801 cgccgcgtac gcgggctggt agcgatgaca ttcgacagcc ggatcaccac gtacctccag
     1861 atcgccaggg accgctatat cacggagctg acccgcgttt gcgcgatcga ctccggctcg
     1921 taccacaagc cgggcatcga tcaggttcag gaccatttcc agcgccgcat gaccgagctc
     1981 ggcttcgagg tcgagcgcgt ccgcaacgcc gaatggggcg acgatctcgt tgcccggcgg
     2041 cgggggaccg gcaagggccg cattctgctc attgggcatg ccgataccgt ctacccggtc
     2101 ggcgaggcgg agaagcggcc ggtcgtaatc gagggcgaca agttgctcgg ccccggcacc
     2161 tgcgacatga aggcgggcat cctgaccgga atctacgcag tcgaagcgct gaacgcgatc
     2221 ggctggaccg att
//
LOCUS       DASSGX010000296         4440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1361298, whole genome shotgun sequence.
ACCESSION   DASSGX010000296 DASSGX010000000
VERSION     DASSGX010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1361298"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..1148
                     /locus_tag="VFI12_05185"
     CDS             192..1148
                     /locus_tag="VFI12_05185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET7055833.1"
                     /translation="MGLARYLMTPTTRRVALKGLIGASAMAHAIKAPFASAQSDDVAP
                     ATFKVPQTLIVGGLDSRQAGEPENSDVLMLARVDLQAKTVRVISIPRDLYIDIPGFGA
                     DKITRAYDFGSKAQNGKFKAGAELMKATIEESFGLTLDGVILTTFHGFENIVDAFGGV
                     DVNNPYEVADSEYPTLDYGYMSIYFPAGEQTLTGEQALQFCRTRHQDGDPGRVMRQQL
                     VLRGLLDKATSADYADQLWDVVKENKKQVRTDLGPSKQLAFALAVPDFSNDSVKFATL
                     DDLVYADTAPNGMWIYSGMWDQIPAFAEGFLAGTIDAPRANS"
     gene            1240..2544
                     /gene="tyrS"
                     /locus_tag="VFI12_05190"
     CDS             1240..2544
                     /gene="tyrS"
                     /locus_tag="VFI12_05190"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HET7055834.1"
                     /translation="MTAQTVDAALRARERGEHVIDYLRRRGYVQDISDEAGLRDLIER
                     EVVTGYIGFDPTAPSLHVGHLLGIMVLGAFQRFGQRPIALAGGGTAMVGDPTGKTAMR
                     RVLTLDEIQVNLQSIVKQFDRYIDFKGGRFGDRSPAALLVNNADWLLQLRYIDFLREI
                     GRHFSVNEMLAAETYKLRLETTGLNFVEFNYRIVQAYDFLHLYRAVGCKLQMGGSDQW
                     GNIVAGVDLIRRVEGEQAFALVTPLLTAASGQKMGKSEGNSIWLDPTMTTPFDYYQYW
                     INSDDADVRKLLLLYTFLPEEQIDELTAVEGEALREAKRALAFESTRLTHGTDAAIEA
                     QEAATRLFSGSRTAGDLLRDEGIPSVDIPRSDVAGISIADLFVRAGLCSSRSDARRMA
                     AQGGLSIGDERLSSVDDPAPSDESALLLRAGKKRFMRVLLTK"
     gene            complement(2918..4216)
                     /locus_tag="VFI12_05195"
     CDS             complement(2918..4216)
                     /locus_tag="VFI12_05195"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7055835.1"
                     /translation="MRASLLGNPVYSNFWLSRLLSQTAQGAILYALLILITDRTDRSI
                     YTSFFVICAIVPALFFGLPAGISVDALPKVPLMVMLNFLRVLFVMALVDANLSLVGIF
                     AVALGLWTIHQFYAPAESSLMASLVDKKLYTQAQSLSNLALSLAQFLGLVLIAPLILR
                     LAGPPALFTVCGMLWIAAAILVGLLPTTRHKAAVNRKKRPSARESLLNGWRLVRTDHA
                     IYEVFIDDMLVGIGGSALVVLMPLYLKGVLNTGAENTVFVFAPAALGLLIGLRSAPIL
                     ARTIGGRRTATMALMLFSATVATFGYVNYVLDFFDNTLNLPVGELARALGLAPVTMMV
                     MLLSVPAGMATSIGSVAARSVLLARTPADMRGQVIATQSLFQNVGALIPTLLAGVAAD
                     VIGVQRVAVAIVIVTAGGALAALTIYRPATARPRISPAQR"
BASE COUNT          842 a         1391 c         1466 g          741 t
ORIGIN      
        1 tggggcgcca tccggtgcgt caaatgtccg gtgcgcagta tgcccgacat ggcccggttc
       61 cgacctgtgc gaaaccatcg cgatggctgg cgcgtaggca atcagggatg gtagggtgtc
      121 cgcgccccgc tgcaattgaa cttgggcccg gccgtcggcc gtggccacga aggtgaggac
      181 agacgttcac tatgggtttg gcacgctatt tgatgacccc gaccacgcgg cgggtcgccc
      241 tcaagggctt gatcggcgca tcggcgatgg cgcacgcaat caaggcgccg ttcgcttcgg
      301 cacagagcga cgacgtcgcg ccggcaacat tcaaggtacc gcaaacgctg atcgtcggcg
      361 ggctggattc ccgtcaggcg ggtgagccgg agaacagcga tgtgctgatg ctcgcgcgcg
      421 tcgatctgca agcgaagacg gtccgcgtga tcagcatccc gcgcgacctg tacattgaca
      481 tccccggttt cggcgcagac aaaatcaccc gcgcctacga cttcggctcg aaagcgcaaa
      541 acggcaagtt caaggccggc gcggagctga tgaaggccac gatcgaggag agcttcggac
      601 tcaccctgga tggcgtcatc ctgaccacct tccacggctt cgagaacatc gtcgatgcat
      661 tcggcggggt cgatgtcaat aacccgtacg aagtcgcgga cagcgagtac cccacgcttg
      721 attacgggta catgtcgatt tacttcccgg ccggcgagca gacgctgacg ggagagcagg
      781 cgctccagtt ctgccggacc cgccaccagg acggcgatcc gggccgggtt atgcgccagc
      841 agctcgtgtt gcgcgggctg ctcgacaagg cgaccagcgc ggactacgcg gatcagctct
      901 gggatgtcgt caaggaaaac aagaagcagg tccggaccga tctcggtccg tccaagcagc
      961 ttgccttcgc cctggccgtc cccgatttct caaatgacag cgtgaagttc gcgacgctgg
     1021 acgatctggt ctacgccgac acggcgccga atggaatgtg gatctattcg ggtatgtggg
     1081 accagattcc ggccttcgcc gagggcttcc ttgccgggac gatcgacgca ccgcgggcca
     1141 atagttgaca agcacgcttt cggatcagta gcgtagacgt cggcagggat ggcgatccct
     1201 gccgattttt ccgcttcgcg caaatcaagg gtttctggaa tgaccgctca gactgtggat
     1261 gccgcccttc gtgcccgtga acgcggagag cacgtcatcg actatctgcg ccgccgcggg
     1321 tatgtccagg atatctccga cgaggcgggc ctacgggacc tgatcgagcg cgaagtggtg
     1381 accggctata tcggcttcga tccgaccgcg ccatcgctcc acgtcggcca tttgctcggg
     1441 atcatggtac tcggcgcgtt ccagcgattc gggcaacggc cgatcgcgct ggcgggcggc
     1501 ggcacggcca tggtcggcga tccgaccggc aagacggcga tgcggcgcgt gctgacgctc
     1561 gacgagatcc aggtcaacct gcagagcatc gtcaagcaat tcgatcgcta cattgacttc
     1621 aagggcggcc gtttcggcga tcgcagcccg gccgccctgc tggtcaacaa cgccgattgg
     1681 ctgctgcagc tcaggtacat cgacttcctg cgcgagatcg gccgccactt ctcggtcaac
     1741 gagatgctcg ccgccgaaac ctacaaactg cggttggaga cgaccggtct caacttcgtc
     1801 gagttcaact accggatcgt gcaggcgtac gactttctgc acctctaccg ggccgtcggc
     1861 tgcaagttgc agatgggcgg ctccgaccag tggggcaaca tcgtcgccgg cgtcgatctg
     1921 atccggcgcg tcgagggcga gcaggccttt gccctggtca ccccgctgct gacggcggca
     1981 tcgggccaga agatgggcaa gagcgagggc aactcgatct ggctcgaccc gacgatgacc
     2041 acgccgttcg actactacca gtactggatc aactcggacg acgccgacgt ccggaaattg
     2101 ctgctgctct acaccttcct gccggaggag cagatcgacg aattgactgc ggtcgagggc
     2161 gaggcactgc gcgaggccaa acgggcgctc gcattcgaat cgacccggct gacgcacggc
     2221 acggacgcgg cgatcgaggc gcaagaggcg gcaacgcggc tcttctcggg cagccggacc
     2281 gctggtgatc tgctgcggga cgagggcatc ccgtcggtcg acattccgcg gtcagacgtg
     2341 gccggcatat cgatcgccga cctcttcgtt cgggccggac tctgctcatc ccggagcgat
     2401 gcgcgacgga tggcggccca gggcggcctc tcgatcggcg acgagcggtt gagctcggtc
     2461 gacgacccag ccccgtcgga tgaatcggcc ttgctcctgc gcgccggcaa gaagcgcttc
     2521 atgcgagtgc tcctgaccaa gtgaccgcac ggagctccgc tccggagccg tagtggcggg
     2581 gctttgtacc cgccttgaac ttctcaatgt atcggtttgg ttgagccggg ccctttccgg
     2641 cgtagcggcg gtgcttgccc cgcccggcgg ttatccccca ggttggttgc cgggggatgc
     2701 ctgcgatcgg cgccaatcga cgtacttgcc gatcacgagg gagcagcaag cggctcccct
     2761 acgtcggaga ggggccggct ccgccaaagg agccggtcgg aaagctcaat gcgggtacat
     2821 cccctctccg gctctcccca tgaggaagag agcgtccccg ccactacgga tcgtttcgag
     2881 ccgggccttg ccgccatgaa aggtattgga tgctggctta tcgctgggcg gggctgatgc
     2941 gggggcgagc ggtggcgggg cgatagatcg tgagggctgc cagcgcgcca ccggccgtga
     3001 cgatcacaat cgcgaccgcc acgcgctgca cgccaatcac gtcggccgcg accccggcca
     3061 gcagcgtcgg gatcagcgcg ccgacattct ggaagagcga ttgcgtggcg atcacctgac
     3121 cacgcatgtc ggccggggtc cgggcaagca ggaccgaccg cgcggccacg cttccaatcg
     3181 acgtcgccat cccggctggc acggacagca gcatgaccat catcgtgacc ggcgcgaggc
     3241 caagcgccct cgccagttcg ccgacgggca gattgagcgt attgtcgaag aagtcgagca
     3301 cgtaattgac gtagccgaag gtcgcgactg tggccgagaa gagcatcagt gccatcgtcg
     3361 ccgtccggcg cccgccgatt gtgcgggcca ggatcggcgc cgatcgcagg ccgatcagca
     3421 ggccgagcgc ggccggggcg aagacgaaga ccgtgttttc ggcgccggtg ttcagcacgc
     3481 ctttcaggta gagcggcatc aggacgacca aggctgagcc gccgatgcca accagcatgt
     3541 cgtcgatgaa gacttcgtag atcgcgtgat ccgtccggac gaggcgccag ccattcagca
     3601 ggctttcgcg cgcgctcggg cgcttctttc ggttcacggc ggctttgtgc cgggtagtcg
     3661 gtaagaggcc aacaaggatc gccgccgcaa tccagagcat gccgcagacg gtgaagaggg
     3721 cgggtggtcc ggccaagcgc aaaatcagcg gcgcgatcag caccagtccg aggaactgcg
     3781 cgagcgacag cgccagattc gagagcgatt gcgcctgcgt gtagagcttc ttgtcgacca
     3841 gcgaggccat cagcgagctt tcggccggcg catagaactg atggatcgtc cagaggccga
     3901 gcgccaccgc gaagatgccg accagcgaca ggttggcgtc gaccaacgcc atcacgaaga
     3961 gcacgcgcag gaaattgagc atgaccatca gcggcacttt cggcagggca tcgaccgaga
     4021 tacccgccgg caagccgaag aacagggccg gcacgatcgc gcagatcacg aagaacgagg
     4081 tgtagatcga ccggtccgtc cgatccgtga tcaggatcag cagggcgtag agaatcgcgc
     4141 cctgcgccgt ctgcgagagg agtcgtgaca gccagaagtt ggaatagacc gggttgccca
     4201 gaagcgacgc gcgcacacgc tccccgaccc acgccgtggg aacgtgggga aggtgcagtc
     4261 ggtgaggctt cggctgtggc actgcggtca ttccgaactc cgagccaagc aacgctcaga
     4321 ttcgagccta ccgcattggg cacgacaggg ctacatccga atcataccga tttgtcagtc
     4381 gctattgccg atgatcggaa gcgcatcctt gatggtgtcc atcgacggac gcacgtcgag
//
LOCUS       DASSGX010000297         2564 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1361324, whole genome shotgun sequence.
ACCESSION   DASSGX010000297 DASSGX010000000
VERSION     DASSGX010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1361324"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="VFI12_05200"
     CDS             complement(<1..916)
                     /locus_tag="VFI12_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055836.1"
                     /translation="MLAVALFAIIPPQTKIKLGLDLKGGVQDILVVHTDDALKLETDT
                     TAEQLQDAVKTANIPGVSVKPQSLTTFTATGVPSGSDQQFRQIAETMTTADYDRSPAP
                     GGGYLFTMKPNVAINNRAQAVTQAIQTIERRVNELGVSEPVVAPYGTAADSIIVQLPG
                     MTDPARAREIIKSTARLELDLVEAGPMSDQAALLQPYGGKVPDGMKLVPSSTGGTPAD
                     PASRPMYLVHAVPAVTGSDLRDAKLSRGDLGRPEVSFTLNSKGSAKFADVTGANIGRF
                     LAIVLDNAVQSAPRIDNRIDTTDARITGS"
     gene            complement(962..1312)
                     /gene="yajC"
                     /locus_tag="VFI12_05205"
     CDS             complement(962..1312)
                     /gene="yajC"
                     /locus_tag="VFI12_05205"
                     /inference="COORDINATES: protein motif:HMM:NF014725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HET7055837.1"
                     /translation="MTQATFPFAVALASPGQAQGNLWVQLLPFALMLLIFYVLVLLPM
                     KRRQKKVQDFQSSVKVGDRVITTSGIYGQITRLGDASVQVQIADKVRVEVARSAIGGL
                     QGQEPVVQSESSGL"
     gene            complement(1313..2461)
                     /gene="tgt"
                     /locus_tag="VFI12_05210"
     CDS             complement(1313..2461)
                     /gene="tgt"
                     /locus_tag="VFI12_05210"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HET7055838.1"
                     /translation="MRFDVTHRDHLARRGRLSLAHGEVETPAFMPVGTRGAVKGLTTD
                     QLHALGAEIILANTYHLHVRPGDDLVARRGGLHRFIGWDRPILTDSGGYQVFSLADRR
                     TLTEEGVVFQSHLDGAPLRLTPETATEIQARLGSDVAMMFDECPSWPAERAAVEASME
                     RTLRWARRNRDRFEAIQTGRAPDVPTPNPGQVQFGIVQGGTYLDLRDRSVAGTLAVGF
                     DAYAIGGLSVGEPVATMYDVVGHTAGQLPEDRPRYLMGTGMPDDLVEAVSRGIDLFDC
                     VLPTRNARNGQLFTRRGPISIKNARYAEDDGPVDPECGCPVCQRHSRAYLRYLFVAGE
                     MSAAVLNTLHNLYFYLDTMGRIRKAIEFGNFEPFRQTFLETYSRRQPV"
BASE COUNT          406 a          851 c          860 g          447 t
ORIGIN      
        1 agctgcccgt gatgcgcgcg tccgtcgtgt cgatgcggtt gtcgatccgc ggcgccgact
       61 gcacggcgtt gtcgagcacg atggccagga accggccgat gttcgcgccc gtcacgtcgg
      121 cgaacttcgc ggagcccttg ctgttgagcg tgaacgagac ttccgggcgg ccgaggtcgc
      181 cgcgcgagag cttcgcgtcg cgcaggtccg acccggtcac ggccggcacc gcgtgcacca
      241 gatacatcgg ccgcgacgcc gggtcggccg gcgtgccgcc cgtggacgac gggacgagct
      301 tcatgccgtc gggcaccttg ccgccgtacg gctggagcag ggccgcttga tccgacatcg
      361 gcccggcttc cacgagatcc agctcgaggc gcgcggtgct cttgatgatc tcgcgtgccc
      421 gcgccgggtc ggtcatcccg ggcagctgca cgatgatcga gtccgccgcc gtaccgtagg
      481 gcgcgacgac cggctcggac acgcccagct cgttcacgcg ccgctcgatg gtctgaatcg
      541 cctgcgtcac ggcctgcgcg cggttgttaa tcgcgacgtt cggcttcatc gtgaacaggt
      601 agccgccgcc cggcgccggg ctgcggtcgt agtccgcggt ggtcatcgtc tcggcgatct
      661 gccggaactg ctgatcgctc cccgacggca ccccggtcgc ggtgaacgtg gtgaggctct
      721 gcggcttgac gctgacgcca gggatgttcg cggtcttgac cgcgtcctgg agctgctcag
      781 cggtggtgtc cgtctcgagc ttcagcgcgt cgtcggtatg gacgacgagg atgtcctgga
      841 cgccgccctt gaggtcgagc cccagcttga tcttggtctg cggcgggatg atggcgaaaa
      901 gcgccaccgc cagcacgcca aaaatgatca gcgccttcca ccgaaggttt ctatacatgt
      961 cttagagtcc gctcgattcc gactgcacga cgggttcctg cccctgcagc ccgccgatag
     1021 ccgaccgcgc cacttccacg cgcactttat ccgcgatctg gacctgcacg gaggcgtccc
     1081 cgagccgggt gatctggccg taaatgccgc tcgtggtgat gacccggtcg cccaccttca
     1141 ccgacgactg gaaatcctgg actttcttct gccgccgctt catcggcagc agcaccagga
     1201 cgtagaagat cagcagcatc agggcaaagg gcagcaactg cacccagaga ttgccctgtg
     1261 cttggcctgg cgaggccagg gcgacggcga atgggaatgt ggcctgagtc atctaaaccg
     1321 gttggcggcg ggaatacgtt tcgaggaacg tttgtctgaa cggctcgaag tttccgaact
     1381 caatagcctt gcgaatacgc cccatggtgt caaggtaaaa gtagaggttg tgcagcgtgt
     1441 tgaggaccgc cgccgacatc tcgcctgcca caaaaagata gcgcaagtag gccctcgagt
     1501 gccgctggca gaccgggcag ccgcattccg ggtccaccgg cccgtcgtct tcggcgtacc
     1561 gggcgttctt gatcgaaatc gggccgcgcc gggtgaacag ctggccgttt cgggcgttcc
     1621 gggtcggcaa gacgcagtcg aacaggtcga tgcctcgcga caccgcctcg accaggtcgt
     1681 cgggcatgcc ggtccccatg aggtaccgcg gccgatcttc gggcaactgc cctgcggtat
     1741 ggccgaccac gtcgtacatc gtggcgaccg gctcgccgac cgacagcccg ccgatggcgt
     1801 aggcgtcgaa gcccaccgcc agggtgccgg ccacgctgcg gtcccttaaa tcaaggtagg
     1861 taccgccctg cacgatcccg aattggacct gaccgggatt cggcgtcggc acgtcgggcg
     1921 cccggccggt ctgaatcgcc tcgaagcgat cgcgattccg cctcgcccac cgcagcgtcc
     1981 gttccatcga cgcctcgacc gcggcgcgct ccgccggcca gctcgggcac tcgtcgaaca
     2041 tcatcgccac gtcggatccg agccgcgcct ggatttcggt cgccgtctcc ggcgtcagcc
     2101 gcagcggcgc gccgtcgaga tgcgattgaa agacgacgcc ctcttcggtg agcgtgcggc
     2161 gatcggccag actgaagacc tgatagccgc ccgagtccgt caggatcggc cggtcccagc
     2221 cgataaaccg gtggaggccg ccgcggcggg cgacgagatc gtcgccgggc cggacgtgca
     2281 ggtggtacgt attggcgagg atgatctcgg cgccgagcgc gtgcagttga tcggtggtga
     2341 gccccttgac ggcgccgcgc gtgcccaccg gcatgaacgc cggcgtctcg acctcgccat
     2401 gcgcgagcga caagcgcccg cgccgggcga gatggtcgcg atgggtgacg tcgaaccgca
     2461 cggcctatga tgccgattca tcggccatct gtcatcggtg atcggtcatc gtattggtta
     2521 tcggggatcg gcgatcgcga ttgagtgatc ggtgatcgag cggt
//
LOCUS       DASSGX010000298         5128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_816460, whole genome shotgun sequence.
ACCESSION   DASSGX010000298 DASSGX010000000
VERSION     DASSGX010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5128
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_816460"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..713)
                     /locus_tag="VFI12_05215"
     CDS             complement(126..713)
                     /locus_tag="VFI12_05215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7055839.1"
                     /translation="MSDDVELVARARAGDTVAFERLFERYHAPVLNYLHRMVGDRALA
                     EDLTQDAFVKAYKALPNTRPELAFKAWLYRIATNTAISHLRRRKLIRWVPFLGTQDYA
                     SNESIERSVGRKHDVEQALRQLPEHYVAVLILRHYEGLSLAETADALGITENAAKLRL
                     FRARKAFANVYTEPVEEEIPVDLETAELDRPEGAR"
     gene            1050..1322
                     /locus_tag="VFI12_05220"
     CDS             1050..1322
                     /locus_tag="VFI12_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055840.1"
                     /translation="MAHVGQPGHLIGQGGSFADALAAMLDALVPLLGPVASPADGDRS
                     ARLQAEAESLPALAADMIDALVGAAQDHDAAPTSVILDGFRAVAGG"
     gene            1551..1745
                     /locus_tag="VFI12_05225"
     CDS             1551..1745
                     /locus_tag="VFI12_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055841.1"
                     /translation="MSGQALVYFGISGGTLLMAFSLLRKRYIERDRSAVTSRGGYTMF
                     AILMLLFAVGAFLAGYFSRS"
     gene            1754..2542
                     /locus_tag="VFI12_05230"
     CDS             1754..2542
                     /locus_tag="VFI12_05230"
                     /inference="COORDINATES: protein motif:HMM:NF015402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HET7055842.1"
                     /translation="MPESRAWPRIVGVLHLPPLPGSARGGDACSIGGIIDAMRRDAAI
                     YQEAGIDTVIVENYGDLPFVRDRVDAAVTSAMTRVVDAVQRDFGLTVGVNVLRNDVLT
                     AVSIAAMTGARFVRANVYVGVMQTESGIIQGCAHAVQQELKRLGAEVEVWADLDVKHA
                     APLVERSIEEQADDAIERAFASALILTGPATGAAADPGAVRRLRTHTDASIYVGSGAT
                     PESIPTMPGATGFIVGTAMKVDGVVTNPVDPARVHAVLAAAQRT"
     gene            2551..3036
                     /locus_tag="VFI12_05235"
     CDS             2551..3036
                     /locus_tag="VFI12_05235"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7055843.1"
                     /translation="MSNPEDTLVRSSFVRIPSEARQASNQRYSMADLEKLTNFSSRTI
                     RYYIQEELLRPAHGRGPSATYDRDHLLRLLRIAELKTEVGALDEIRERLNALTTADLE
                     AHFAVKSGPEEERWRRVRIHPRLELNVREMADRDYRFEQVVEQIVDHARYLLERYESE
                     R"
     gene            3179..4843
                     /locus_tag="VFI12_05240"
     CDS             3179..4843
                     /locus_tag="VFI12_05240"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HET7055844.1"
                     /translation="MDVDLRQLFHSALRYWWLVIALPIALGVMAFLYTARQDPMYRAE
                     VSLEVRASSTNDNAYDILLGSERQAKTYQRLVKDPAVLQAAAQHIQPPMDIQALTLKV
                     GSRVDSGTSLLIISVADTNPESAATIANLIAQELVKVVDARNEESVRQRTTDLQQRIA
                     DLKTEIDTKRAEILELERGPDADTDAVVNRIADLNVDIGRDQDNIQLFQTQIGTVIAN
                     PGDTVRIFSTAVPPREPFAPRKLMNIVLGIMLGLMLAAGAILLIDYLDNTVKATLDFP
                     QIVGGPLLATVRAIDRIKPGHSQLFVLDDPKGVPAESIRLLRANIEFASATRELVSIS
                     LTSPNPGEGKSTISANLAVALAQAGFVTMLIDADLRRPTQHRIFGVANDRGLSSLLAQ
                     QDREWRWAACETMLPNLSVITSGPLPPNPADLLSLDRLRQILEELREAVDVIIIDSPP
                     MLAVSDPLIIAAHVDGTALVSLGGKTRLEALKRAVQILHRGAPRIIGVVLNQQSDKSD
                     GGYYYQEYSAVSDEPRGGFRKRRATTSTAIPATTATISPVEQTSAD"
BASE COUNT          880 a         1738 c         1683 g          827 t
ORIGIN      
        1 gtgcgcggcg agccgctgct cctgctgcgg ggcgagggga gcatcgagcc gggccgagat
       61 cagcgcctcg gcctcggcag ggttgagcgg gtgcgcggcc ggaccgtgac cgcgcgagtg
      121 gccgatcacc gcgcaccctc cggcctatcc agctctgcag tctccagatc caccggaatt
      181 tcctcctcga cgggctcggt gtagacattc gcgaatgcct tccgggcccg gaagaggcga
      241 agtttcgcgg cgttttcggt gatgccgagg gcgtcggccg tctcggccag ggataggccc
      301 tcgtaatgcc ggaggatcag aacggcgacg tagtgctccg gcagttggcg cagcgcctgc
      361 tcgacatcgt gcttgcggcc gaccgagcgt tcaatcgatt cattcgacgc atagtcctga
      421 gtccccagga acgggaccca gcggatcagc ttgcgccgac ggaggtgaga gatcgcggtg
      481 ttggtggcga tccggtagag ccaggccttg aaggcgagct ccggccgggt attcggcaat
      541 gccttgtacg ccttgacgaa ggcgtcctgg gtgagatcct cggccagggc gcgatcgccg
      601 accatgcggt gcagatagtt gagcaccggc gcgtggtacc gctcgaacag ccgctcaaac
      661 gcaacggtgt cgccggcccg cgcccgggca actaattcga cgtcatcgga cacggggtaa
      721 ctccgtgccg gggccggggg cgccggtgag gcgccgccca gccgaaaact gctggtttgt
      781 atcgggggtg ccaacgcgtc tgccatcgac taatcgatcc ctctccttcg cagcccaaaa
      841 cgcgccaccg gcgaggccgg tttcgctgat tgaaaactgc gcatggaaag gcgggattcg
      901 atcccgtgac ggagcgccca tgagttccgg agaataccaa atggccgggg agaactgccg
      961 gatcgccggc cgctccatgt ttcacatgaa acataatctg cggaatcctg cccgggagcg
     1021 cccggccggg atcgacacgg ggtgctgaaa tggcgcatgt tgggcaaccg ggacacctga
     1081 tcgggcaggg cggctcgttc gccgatgcgc tcgcggccat gctcgacgca ttggtcccgt
     1141 tgctcggacc ggtggcatct ccggctgacg gtgaccgctc tgcgcggttg caggcagaag
     1201 cggaatccct gccggctctc gccgccgaca tgatcgacgc gttggtcggg gccgcgcagg
     1261 atcacgacgc ggcgccgacc agcgtcatcc tcgacggctt ccgcgcggtc gcgggcggat
     1321 agcgggcatg ggcgacggcc acgctcagcc tgacagcgcg gcagtcactc gctgttccaa
     1381 ccgtcgatgg gctgacggtc agccaagcgg acagcgggtg gcagatttcg gccgcagtcg
     1441 tgatcgaata gagccgctgt gacaatcagt ccacccgggt ggacgggagc gccggtgccg
     1501 atccggccta caatccccgc aggaatattc gggagcagag acaagggttc atgagcggtc
     1561 aggcactggt ctattttgga atcagcgggg gcacgctgtt gatggcgttc tcgctgcttc
     1621 gcaagcggta catcgagcgg gatcggtcgg cggtcacctc acgtggcggc tatacaatgt
     1681 ttgcgatctt gatgctgctg ttcgcggtcg gcgccttcct cgccggatac ttctcacgga
     1741 gctgatgcca cccatgcccg aatcccgcgc ctggccccgg atcgtcggcg tcctgcacct
     1801 gccgccgttg cccggctcgg ctcggggtgg cgatgcctgc tcgatcggcg ggatcatcga
     1861 cgcgatgcgc cgggatgcgg cgatctacca agaggcgggg atcgacacgg tcatcgtcga
     1921 gaactacggc gatctcccct tcgtgcggga ccgggtggat gcggcagtga ccagcgccat
     1981 gacccgggtg gttgatgcgg tccagcgcga ttttggcctg acggtcggcg tcaacgtgct
     2041 tcgcaacgat gtcctgactg ccgtctcgat tgcggcgatg accggcgccc gcttcgtccg
     2101 ggccaacgtc tacgtcggcg tgatgcagac ggagtcgggg atcatccagg gctgcgcgca
     2161 cgcagtccag caggagctga agcggctcgg cgcggaggtc gaggtctggg ccgacctcga
     2221 cgtcaagcat gccgctccgc tcgtcgaacg ctcgatcgag gagcaggccg acgacgccat
     2281 cgagcgcgcc ttcgcctcgg ccctgatcct caccggcccc gcgaccggcg cggcagctga
     2341 tcctggcgcg gtccgcaggc tgcggacgca taccgatgcg tcgatctacg tcggcagcgg
     2401 ggccacgccg gaatcgatcc cgaccatgcc cggcgcaacc ggcttcatcg tcggcacggc
     2461 gatgaaagtc gatggcgtcg tgaccaaccc ggtcgatccg gcccgggtgc acgcggtcct
     2521 cgccgcggcc cagcggacat gagcgcggca atgagcaacc ccgaggacac gctggtccgc
     2581 tccagcttcg tgcggattcc ttccgaggca cgccaggcct cgaatcagcg ctattcgatg
     2641 gccgatctcg agaagctgac caatttctcg agccggacca tccgctacta catccaggaa
     2701 gagctgctgc gcccggccca cggtcgcgga ccgtccgcaa cctacgatcg cgaccacctc
     2761 ttgcgcctgt tgcggatcgc cgagctcaag accgaggtcg gcgcgctcga cgagatccgg
     2821 gagcggctga acgcgctcac gaccgccgac ctcgaggccc atttcgcggt caagagcggc
     2881 ccggaggaag agcgctggcg gcgggttcgc atccaccccc gactggagct caacgtccgc
     2941 gagatggccg accgcgatta ccggttcgag caggtcgtcg agcaaatcgt cgaccatgcc
     3001 cggtatctgc tcgaacgata cgagagcgag cggtaggggt ccgtcccggc caatccggga
     3061 ctttcgggcg ttcccgggac ccccaattgc cttcccccgt ccctagactg aatctggcgg
     3121 aaaccggcgg ccctgccctc cgcgtgtctc actgctcgat ccggacgggg acctgcattt
     3181 ggacgtcgat ctccgacaac tgttccacag cgcgctcagg tactggtggc tcgtcatcgc
     3241 cttgccgatc gcgctcggcg tcatggcgtt cctctacacc gcgcgccagg atccgatgta
     3301 ccgcgccgag gtgtcactcg aagtgcgcgc ctcgtcgacg aacgacaacg cctacgacat
     3361 cctgctcggc tccgaacgac aggccaagac ctatcagcgc ctggtcaagg acccggcagt
     3421 gctgcaggcc gcggcgcagc acatccaacc gccgatggac atccaggcgc tgacgctcaa
     3481 ggtcggcagc cgggtcgatt ccggcacttc actcctgatc atcagcgtcg ccgacaccaa
     3541 ccccgaatcc gccgccacga ttgccaatct gatcgcgcag gaactggtca aggtcgtcga
     3601 cgcccgcaac gaggagagcg ttcgccaacg gacaacggac ctccagcagc ggattgccga
     3661 cctgaagacc gagatcgaca cgaagcgggc cgagatcctc gagctcgagc gtgggcccga
     3721 tgccgacacg gacgcggtcg tcaaccggat cgccgacctc aatgtcgaca tcgggcgaga
     3781 tcaggacaat atccagctct tccagaccca gattggcacc gtgatcgcca acccgggcga
     3841 tacggttcgc atcttcagca cggccgtgcc gccgcgcgag ccgttcgcgc cccggaagtt
     3901 gatgaacatc gttctcggga tcatgctcgg gctgatgttg gccgccggcg cgatcctgct
     3961 gatcgactac ctggacaaca cggtcaaggc gacgctcgat ttcccccaga tcgtgggtgg
     4021 gccgctcctg gccacggtcc gcgcgatcga ccggatcaag cccggccact cgcagctctt
     4081 cgtgctcgat gacccgaagg gagtcccggc cgaatcgatc cggctattgc gggccaacat
     4141 cgaattcgcc tcggcgacgc gcgaactggt ctcgatcagc ctgacgagcc ccaacccggg
     4201 cgagggcaag agcacgattt ccgccaacct cgccgtggca ctggcccagg ccgggttcgt
     4261 gacgatgctg atcgacgccg acctccggcg cccgacccag caccgcatct tcggcgtcgc
     4321 caacgatcgc ggcctgagca gcctgctggc ccagcaggac cgggagtgga ggtgggcggc
     4381 gtgcgagacg atgctgccga acctgtcggt gatcacgtcg ggaccgctgc cgccgaaccc
     4441 ggccgatctg ctcagcctcg atcggctccg ccagatcctg gaagagttgc gggaagctgt
     4501 cgacgtgatc atcatcgact cgccgccgat gctggccgtg agcgaccccc tgatcatcgc
     4561 cgcccacgtc gacggcacgg cactggtcag cctcggcggc aagacacggc tggaggccct
     4621 caagcgggcc gtccagatct tgcaccgggg cgcgccgcgg atcatcggcg tcgtgctcaa
     4681 ccagcagagc gacaagagcg atggtggcta ttactaccag gagtacagcg cggtctcaga
     4741 cgaaccccgc ggcggtttcc gcaagcgccg ggccaccaca tccaccgcga tcccggcaac
     4801 gaccgcgacc atctccccgg tcgagcaaac ctcggcagac tgacctcagt acgcgccctg
     4861 ccatcgcttg cagggcgatg atcgttgccg ggaggcgggg tcgggatatc caacgggccg
     4921 tccggcgccg cccgggcacg aacgtccgct tgcgaatggc cgggtggatg agcgaagggc
     4981 agatgccatc tgcccctact gttccctcaa ccctcaaccc tcaaccctca ccccgggatg
     5041 gtgtcagccg aagtggcgct gctcggcggc gccccggcgg tcgccggtca cgacccacgg
     5101 cttgatctcg gccgagagcg cagggtcc
//
LOCUS       DASSGX010000299         2096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_707034, whole genome shotgun sequence.
ACCESSION   DASSGX010000299 DASSGX010000000
VERSION     DASSGX010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707034"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="VFI12_05245"
     CDS             <1..309
                     /locus_tag="VFI12_05245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HET7055845.1"
                     /translation="IYTGLFQTPEMIAVAALQEDVQVVGLSILSGAHLSLFPKIITEL
                     RTRGLDHVLLVAGGTIPPRDVQGIKDLGVDAVFGPGSSLSSIVEYIRDHAPQPATETL
                     "
     gene            310..1224
                     /gene="meaB"
                     /locus_tag="VFI12_05250"
     CDS             310..1224
                     /gene="meaB"
                     /locus_tag="VFI12_05250"
                     /inference="COORDINATES: protein motif:HMM:NF015279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HET7055846.1"
                     /translation="MGLFDRARAGDQWALARLATLIENDRPDAVAALDALGAPQRPLH
                     VIGVTGPPGAGKSSLINHLLTELRKEGTRIAVLLVDPSSQATGGAVLGDRVRMLAWGD
                     PDVFVRSQATRGQEGGLAPSTATLIDLFAHMDFETVLIETVGVGQDGIDIRAVCDTTI
                     VVQAPNLGDSIQALKAGILEIADIFVVTKSDLPGAHQVVRDLNAMLHLIGTGEQNWPV
                     PVVAVSSTEETGFETLGAKITSHRSHIGCQTAPDRRAARMRWEIAKRATARLAATARR
                     LPPEAWTASGSRDDRTRALLQATLDEYE"
     gene            complement(1250..1993)
                     /gene="larB"
                     /locus_tag="VFI12_05255"
     CDS             complement(1250..1993)
                     /gene="larB"
                     /locus_tag="VFI12_05255"
                     /inference="COORDINATES: protein motif:HMM:NF033503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HET7055847.1"
                     /translation="MRPFDDLRAALDGASDESAGQSVRSDTRRSARTGTPEVIYAAHK
                     SIEQILAGIEGLIDVRDRVVVSKIGTERAQALREALTGDLSLSLAPGNLSGVVFRRES
                     APPSGGGRIGVISAGTSDIPVAAESALIATEMGCDVHTTWDVGVAGIHRLIRPLEAMT
                     EWDADVFVVAAGMDGVLPSVVSGLVAQPVIGLPISSGYGFGGEGIGALTTMLQTCSPG
                     IAVVNIDNGIGAGITAARIANRAALARRA"
BASE COUNT          368 a          763 c          638 g          327 t
ORIGIN      
        1 atctacaccg gcttgttcca gacgccggag atgatcgccg tggccgccct ccaggaggac
       61 gtgcaggtgg ttgggctgag cattctctcc ggcgctcacc tctcgctctt cccgaagatc
      121 atcaccgagc tgagaacgcg cggactcgac catgtcctgc tcgtggccgg cggcacgatc
      181 ccgccccggg acgtgcaggg gatcaaggat ttgggcgtcg atgcggtctt cggaccgggg
      241 tcgtcgctct cctcgatcgt cgagtacatc cgtgatcacg ctccgcaacc ggccaccgag
      301 acgctctaga tgggcctctt cgaccgcgcc cgggctggcg accagtgggc cctcgcccgc
      361 ctcgcgacgt tgatcgagaa tgaccggccc gacgccgtcg cggcgctcga tgcccttggc
      421 gctccacagc ggccgctcca cgtgatcggc gtcaccggac ctcctggagc cggcaagagc
      481 tcgctgatca atcacctgct gaccgagctg cgcaaggagg gaacgcggat tgccgtgcta
      541 ttggtcgatc cgtcgagcca ggccaccggc ggcgcagtcc tcggcgatcg ggtccggatg
      601 ctggcatggg gcgatcccga cgtattcgtg cgcagccagg cgacccgcgg ccaggagggc
      661 gggcttgccc catcgactgc gaccctgatc gacctcttcg cgcacatgga cttcgagacg
      721 gtcctgatcg agacggtcgg tgtgggccag gacggcatcg acatccgcgc ggtctgcgat
      781 acgacgatcg tggttcaggc gccgaatctc ggcgacagca tccaggcgct caaggccggc
      841 atcctcgaaa tcgccgacat ttttgtcgtc acgaaatccg acttgcccgg ggcgcaccag
      901 gtcgtccggg acctgaacgc aatgctgcac ttgatcggaa ccggcgagca gaactggccg
      961 gtcccggtcg tggctgtctc gtcaaccgag gaaaccggct ttgagaccct gggagcgaag
     1021 atcacctcgc atcgatcgca catcggctgc cagaccgccc cagaccgccg cgctgcccgg
     1081 atgcgctggg agatcgcaaa gcgcgccacc gcccgcctcg ccgccaccgc tcgccgcctc
     1141 ccacccgaag cctggacagc atctggctca agagacgacc gaacccgcgc actgcttcag
     1201 gcaacgctcg acgagtacga gtagagtcgc atagcaacgc gagccggggc tacgctcgcc
     1261 gcgccaacgc cgcccggttc gcaatccgcg ccgccgtaat gcccgcccca atcccattgt
     1321 cgatattgac caccgcaatc cctggcgaac acgtctggag catcgtcgtc agcgccccga
     1381 ttccctcgcc tccgaacccg tagccggacg agatcggcag cccgatcacc ggctgcgcca
     1441 ccaaccctga cacgaccgac ggcagcaccc cgtccatccc cgccgcaacg acgaagacgt
     1501 cggcatccca ctcggtcatc gcttcgaggg gccgaatcaa ccgatggatg ccggccaccc
     1561 cgacatccca cgtcgtgtgc acgtcgcagc ccatctcagt cgcgatcagg gccgattcgg
     1621 ccgccaccgg aatgtcggac gtcccagcgc tgatcacgcc gatccgcccg ccgccgctcg
     1681 gcggcgcgga ttcccgacga aacacgacgc ccgacaggtt gcccggcgca agggagagcg
     1741 agaggtcgcc ggtcagggcc tcacgcaacg cttgtgctcg ttccgtgccg atcttggaca
     1801 cgacgacgcg atcccggaca tcgatgagcc cttcgatgcc ggccaggatc tgctcaatcg
     1861 acttgtgagc ggcgtaaatc acctcgggcg tgccggtccg cgccgagcgt cgggtgtcgg
     1921 agcggaccga ttgcccggcc gactcgtctg acgcgccatc gagcgccgcg cgaagatcgt
     1981 caaacggccg cattccccaa ccccacctgt tccagcaaat tgtctatgcg caccgggtcg
     2041 ccctctcgat aatgaagtgc ggccagaaac tcgatattcc cggccggccc ggtcaa
//
LOCUS       DASSGX010000300         2487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_382103, whole genome shotgun sequence.
ACCESSION   DASSGX010000300 DASSGX010000000
VERSION     DASSGX010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382103"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="VFI12_05260"
     CDS             complement(<1..779)
                     /locus_tag="VFI12_05260"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7055848.1"
                     /translation="MPEGEDQIVANEGVVSVPTERTISRRRLVRNSALTAGALALASQ
                     IDVKYALGQDEGPIKIGWLGDRTGDFSVVGVQKFNAANLAVKELNDAGGVLGRELVLV
                     AEDAQSDNQRYQQMARKLILQDKVDVLHACFTSASREAIRPIVDQNKMLFFYNNQYEG
                     GVCDSWVFPTGCVPDHQVVPFMPAVMEKFGPRVYTIAANYNFGQLTADWCRKIVEQQG
                     GEIIGEEFIPLDVSQFSTSITNIQNAKPDVLIVLLVGIKQSS"
     gene            complement(1062..1322)
                     /locus_tag="VFI12_05265"
     CDS             complement(1062..1322)
                     /locus_tag="VFI12_05265"
                     /inference="COORDINATES: protein motif:HMM:NF015630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HET7055849.1"
                     /translation="MSLIYINIGTELAELLGDTPEEIERRARESIVLELYRRRDLPAG
                     HAAKALGLDEVRFVRWAGEQGVPDLDMAPEGWARELRANNKE"
     gene            complement(1408..1839)
                     /locus_tag="VFI12_05270"
     CDS             complement(1408..1839)
                     /locus_tag="VFI12_05270"
                     /inference="COORDINATES: protein motif:HMM:NF023276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3368 domain-containing protein"
                     /protein_id="HET7055850.1"
                     /translation="MISLHQIGQRTLLEALFDEIMELPAVAREVAPSVGLLPLWIRVI
                     RAPALSGLPRPLGPGPRAAIALAVHLAAHYIVVDDAQGRLAAEARGLKPVGSLGLLVR
                     ARRRGLIETVRPRMDAMIAAGLYVSPRVYTEILEAAGEPGP"
     gene            complement(1872..2132)
                     /locus_tag="VFI12_05275"
     CDS             complement(1872..2132)
                     /locus_tag="VFI12_05275"
                     /inference="COORDINATES: protein motif:HMM:NF015630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HET7055851.1"
                     /translation="MGQTQVALDKRVLEVLGDTAEQIERGAIEAIVLDLYRRRQISVG
                     RAAEILGLDQLSFIRWSGALGVPFFDMTPEEWQRELRTIESM"
     gene            complement(2210..>2487)
                     /locus_tag="VFI12_05280"
     CDS             complement(2210..>2487)
                     /locus_tag="VFI12_05280"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET7055852.1"
                     /translation="AEELWERLGMPYSVHHQAWPEYDAALTQEETVEVVVQVNGKVRD
                     RLSLPVDLPESEARQRALAQSKIAETLNGREPTKVIYVPGRLVNIVV"
BASE COUNT          463 a          854 c          719 g          451 t
ORIGIN      
        1 gacgactgct tgatcccgac cagcagcacg atcaggacat ccggcttggc attctggatg
       61 ttcgtgatcg acgtgctgaa ctgcgagacg tcaagcggga tgaactcctc gccaatgatc
      121 tcgcccccct gctgctcgac gatcttgcgg caccagtccg ccgtcaactg gccgaagttg
      181 tagttagccg caatcgtgta gacgcgggga ccaaactttt ccatgacggc cggcatgaac
      241 ggcacgacct ggtggtcagg cacgcacccg gttggaaaga cccaggaatc gcagacgccg
      301 ccttcatatt gattgttgta gaagaagagc atcttgttct gatcgacaat cggccgaatc
      361 gcctcgcgag acgccgaggt gaagcaggca tggaggacgt cgaccttgtc ctgcaggatg
      421 agcttgcggg ccatctgctg gtagcgctgg ttgtccgact gcgcgtcctc ggccaccagc
      481 accagctcgc gcccaagcac gccgccggcg tcgttcagct ctttcacggc cagattcgcc
      541 gcgttgaact tttgcacgcc gacgaccgag aagtcgccgg tgcgatcgcc gagccacccg
      601 attttgatcg gcccctcgtc ctggccgagc gcgtacttca cgtcgatctg cgacgcgagc
      661 gccagcgctc ccgccgtcag cgccgaattc cgcacgagcc ggcgccgcga aatcgttcgc
      721 tccgttggca ccgaaaccac gccctcgttc gccacgatct ggtcctcccc ttcaggcacc
      781 gagcaggctc cttccgccgc accgcgcgac ggcgcgtccg cttgtccagg agcggcatgt
      841 tggaagcgac ttgccgtcaa cgcatgcgac atcgtacgtc tggtcgccgc aggctccagc
      901 acctgtctcc cctgatccaa gctacgtggc ggaaacctag caaaccggct ggattctgtc
      961 caaccgagcg tttcgtcagt cttggcgcgg tcatgcacga cgccgagggg ccaggagatc
     1021 gaccgtgcgg gaggaccgcc ggaagccaac gtcgtcagaa gtcattcctt gttgttggcg
     1081 cgcagttccc gtgcccagcc ttccggcgcc atgtcgagat cgggcactcc ttgctcgccg
     1141 gcccagcgaa cgaacctgac ctcgtcgagc ccgagagctt tggcggcatg cccggccggc
     1201 agatctcgcc ttcggtaaag ctcgagcacg atcgattccc gcgcgcgacg ctcgatttcc
     1261 tcgggtgtgt caccaagcag ctctgcgagt tcggtgccga tgttgatata aatcaggctc
     1321 atatcccgcc tcgttgtcgc agtcccaggt cgcaagcaga ctgccagttc gaagcgcacg
     1381 cgatgacgtt gcacctccgc tacgggtcta tgggcctggt tcgccagcgg cttccaaaat
     1441 ctcggtgtat acacgtgggc tgacatacag ccccgccgca atcatcgcgt ccatccttgg
     1501 ccggaccgtc tcgatcagcc ctctccgccg ggcgcggacg agcaatccca gcgaccccac
     1561 cggcttcaac ccgcgggcct ccgcggcgag gcgcccctga gcatcgtcaa ccacgatgta
     1621 gtgcgcggcg agatgcacag cgagagcgat cgccgcgcgc ggtccaggtc cgagcggacg
     1681 tggcagacca gacagcgcgg gtgctcgaat cacacggatc caaagcggca gcagtccaac
     1741 ggatggtgca acttccctcg ccacagcagg cagctccatg atctcgtcga agagagcttc
     1801 cagcaatgtt cgttggccga tctgatgcag cgagatcaaa gggctcgcgt ccgagaccgc
     1861 gacctcagga ctcacatcga ttcgattgta cggagttccc gctgccactc ctccggggtc
     1921 atatcgaaga aaggcacacc gagagcgcca gaccaccgga tgaatgaaag ttgatcgagt
     1981 cccaggatct cggccgcgcg accgaccgaa atttgacgac ggcgatagag atcgagaacg
     2041 atcgcctcta tggcgccccg ttcaatctgt tccgccgtgt ctccgagtac ctctaacaca
     2101 cgcttgtcga gggcaacttg cgtttgaccc atggaagacc ctccaacaag tgatgacacg
     2161 cgcgcatgat agcggatcga tcacggcgcc attccgacct ttccggcgcc tacaccacga
     2221 tattgaccag ccgccccggc acgtagatca ccttcgtcgg ctcccggcca ttcagcgtct
     2281 cggcgatctt cgactgcgcc agcgcccgct gccgcgcctc ggattccggt agatccaccg
     2341 gcagcgacag gcggtcgcgc accttgccgt tgacctgcac cacgacctcg accgtctcct
     2401 cctgcgtcag cgccgcgtcg tactcgggcc acgcctggtg gtggacggag tacggcatcc
     2461 ccagccgctc ccatagctcc tcggcca
//
LOCUS       DASSGX010000301         3378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_382109, whole genome shotgun sequence.
ACCESSION   DASSGX010000301 DASSGX010000000
VERSION     DASSGX010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382109"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VFI12_05285"
     CDS             <1..218
                     /locus_tag="VFI12_05285"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7055853.1"
                     /translation="RHFRDTLNSYLGSAFSLEPILTQSAWFRPHNRSEELANLYFVGA
                     GTHPGAGLPGVLCSAVIADNLIAEATA"
     gene            complement(263..1207)
                     /locus_tag="VFI12_05290"
     CDS             complement(263..1207)
                     /locus_tag="VFI12_05290"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HET7055854.1"
                     /translation="MKRIALAGARAAASRLDGEGGGCANLRADIDWRCCTEIAREHGR
                     TFYHASLFLPKARRQAVHATYAFCRIADDIADRSPNPEIASRDLDEWERQIDEPTDPV
                     AVAFAAARNRYGVPTAAVRELLAGVRMDLVPRRYSDWDDARVYCYRVAGTVGLMVAPI
                     LGCLDTAALPHAVNLGIAMQWTNILRDIGEDARRGRLYLPLEDLTAFGCDPEALMREQ
                     ATGRFVDLLDYEIARARRLYADAHHGFPALSPSGRLTALAGCELYGAILTRIEENGYD
                     VFARRAHISTRRKLAAMPGIAATFMRLSWQPRPSGFSG"
     gene            1796..2929
                     /locus_tag="VFI12_05295"
     CDS             1796..2929
                     /locus_tag="VFI12_05295"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7055855.1"
                     /translation="MEKTYAARPLPVQALRGVDLTIGQGSLTAILGASGCGKSTLLKI
                     IAGIERQDRGEITLAGLQIAADGMHTPPEQRDIGIVPQEGALFPHLSVAGNIGFGLRS
                     WRGNPVSLRARRERGRRVDELLDLVGLAGYGNRRPDELSGGQQQRVALARALAPSPKL
                     ILLDEPFSALDAGLRAELRLEVRDLLRRAGTTSILVTHDQEEALSLADHVSIMRDGVV
                     VQSGSPHEIYTRPADPTVASFVGDAVLLPAMIDGGHAEMAVCELGCVAVCASCRWLGR
                     GPCTLMVRPEQVVLDADGLPGRILGFTFYGHDTMITIGMGEDGSGSRVAARVPKGVAP
                     KIGDHVRVRVAGDAIAYADGERRDAEAAFHIPPVLGHGAESFS"
     gene            2984..>3378
                     /locus_tag="VFI12_05300"
     CDS             2984..>3378
                     /locus_tag="VFI12_05300"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET7055856.1"
                     /translation="MRRREHRRAVLKYGAAAIAVGMATTPLRALGQSTPAATPAGSSG
                     TLTIYSGQHEDLANTLAQAFAAATGATVNVRAGSDSDLANQIIEEGDQTNCDVFITEE
                     PGPAAALDAQGLLAPIDIASLAKTDDRFNP"
BASE COUNT          576 a         1091 c         1140 g          571 t
ORIGIN      
        1 cgcggcattt ccgggatacg ctgaacagtt atctcgggtc cgccttctcc ctcgagccca
       61 tcctcaccca gtcggcgtgg ttccggcctc acaaccgttc ggaagagctg gcgaatctct
      121 acttcgtggg cgcgggcacg cacccgggcg ccgggctgcc cggcgtcctc tgctcggccg
      181 tcatcgccga caacctgatc gccgaagcga cggcatgatc tgaacgccgg ggcgagggga
      241 tgttgcccat ccgttcgcca gctcatccgg aaaagccaga cggccggggc tgccaggaaa
      301 ggcgcatgaa tgtcgccgcg atgcccggca ttgccgcgag tttgcgccgg gtagaaatgt
      361 gtgctcggcg tgcaaacacg tcgtatccat tttcttcgat ccgggtcaga atcgccccat
      421 agagttcaca accggcgaga gctgtaagtc gaccggaagg cgacagcgct gggaacccgt
      481 ggtgcgcatc ggcatacagg cggcgcgccc gggcgatctc atagtcgagc agatcgacga
      541 accgcccggt cgcctgctcc ctcatcagcg cctccgggtc gcacccgaat gccgtcagat
      601 cttccagggg cagataaagc cttccgcgac gcgcatcctc tccgatgtcg cgcaggatat
      661 tcgtccattg catggcgatc cccaaattta ccgcgtgggg cagggcagcg gtatccagac
      721 aaccgagaat tggggcgacc atgagcccga cggtgcccgc gacccggtag caatacacgc
      781 gtgcatcatc ccagtcgcta taccggcgcg ggaccaggtc catgcggacc ccggccagca
      841 gttcgcgcac ggccgccgtc ggcacgccat agcgattccg tgccgcggca aaggcgacgg
      901 caacgggatc cgttggttcg tcgatctgtc gctcccactc gtcgagatcg cggctggcga
      961 tctccggatt aggggagcgg tcggcgatgt cgtcggcaat gcggcagaac gcgtaggtcg
     1021 cgtggaccgc ctgtcgccgt gccttgggca gaaacaggct cgcgtggtag aaggtgcggc
     1081 cgtgctcgcg agcgatctcc gtgcagcacc gccagtcaat gtctgcgcgg aggttcgcgc
     1141 atccccctcc ctccccatcg agccgggagg cagcggcgcg ggcacccgca agggcgatgc
     1201 gcttcatggg tggtgcctca tgtgatgcgt cgccgtccag tccctcacgc acggtgcgcc
     1261 ggctcgactg atctcgcagt gcacacccaa gtgcaattcg cgtgccgccc ggtggaatgc
     1321 ttccgtgaag agcgccgtct cagcggcgac cggggatgga cccgttgtcg tggatcccga
     1381 acgggacaaa ggccgggagc caccgaccgc gggagaacgg accggtacgc gtggcgccgt
     1441 catcgccaac cgaggtccgc atcgaccaga acgatgcctg atagtacgtg attccaggct
     1501 gccttttctt tcttgaaatg cgtggatccc tggccgtctt gatcggtcac tcctggtcgc
     1561 gcggcgggtg tacaccagca cagggagggc gaatacgacg gccgcaacct ccgcttgacc
     1621 ggaaagcaca gtgcccctat gcgaaatccg acctggctgg tatgatttcg ccatatgatg
     1681 agcgggtgcg ctcaagaccg gaaatcgttg cccagggtcg aggatgatga ccgcggtctc
     1741 ttcgaacgaa tcaaccgtcg ccgcgagcga gcgtgactgg ctgacggtgc gtggcgtgga
     1801 aaaaacgtat gccgcgcggc cgttgcccgt gcaggcattg cgcggcgtcg atctgacgat
     1861 cggtcagggg agtctgacgg cgatcctcgg cgcgtcgggc tgtggcaagt cgacgctgct
     1921 gaagatcatc gccggtatcg agcgacagga tcggggcgag atcacgctcg ccggtctcca
     1981 gatcgcggcc gacgggatgc atacccctcc ggagcaacgc gacatcggta tcgtgcccca
     2041 ggagggcgcg ctctttccgc atctcagcgt cgcgggcaat atcgggttcg ggttgcgctc
     2101 ctggcgcggc aatcctgtct ccctgcgtgc gcggcgggaa cgcgggcggc gcgtcgacga
     2161 gctgctcgat ctggtaggtc tggcgggcta cggcaaccgc cgcccggatg aactctccgg
     2221 cgggcagcaa cagcgcgtcg ctctggcgcg agccctggcg ccatccccca agctgatttt
     2281 gctggacgag ccgttctcgg cgctggatgc cggcttgcgg gccgagctgc ggctggaggt
     2341 gcgggacttg ctgcgccggg ccggcaccac atcgatcctc gtgacgcacg atcaagaaga
     2401 ggcgctctcg ctggcggacc acgtgtcgat catgcgcgac ggtgtcgtcg tgcagtccgg
     2461 atcgccgcac gagatctaca cccgaccggc cgatccaacg gttgccagct tcgtcggtga
     2521 cgccgtcctg ctgccggcca tgatcgatgg cggccatgcg gaaatggcgg tgtgcgaact
     2581 tggctgcgtc gcggtctgcg cctcgtgccg ttggctcggt cgggggccat gcacgctgat
     2641 ggtacgtccg gagcaggtcg ttctcgacgc ggacggcctc ccgggacgga ttctcgggtt
     2701 cacgttctac ggtcacgaca cgatgatcac gatcggtatg ggcgaagacg gctcgggatc
     2761 cagagtcgcc gcgcgcgtgc cgaaaggggt cgcgccgaag atcggcgacc acgtacgcgt
     2821 ccgcgtcgcg ggcgatgcca tcgcctacgc cgacggcgaa cgacgcgacg ccgaagcggc
     2881 atttcacatt cccccggtgc ttggtcacgg ggccgaatca ttctcatagg tcaacctccc
     2941 gacccggcaa ctcccggatc ggtcggcaaa ggaggagtgc gtgttgcgga gacgcgaaca
     3001 tcgtcgtgcg gtgctgaagt acggggcggc cgcgatcgcg gtggggatgg cgactacgcc
     3061 cttgcgggcg ctggggcaaa gcacgccagc ggcgacgccg gctggctcat ccggaacgct
     3121 gacgatctat tccggccagc atgaagacct ggcaaatacc ctggcgcagg cgtttgcggc
     3181 ggcaacaggg gccacggtca acgtgcgcgc ggggagcgat tccgatctcg cgaaccagat
     3241 catcgaggag ggcgaccaga ccaactgcga tgtctttatc accgaggagc ccggtccggc
     3301 ggcggcgctc gacgcacagg gtttgctggc gccaatcgac atcgcgtccc tggcgaagac
     3361 cgacgaccgt ttcaaccc
//
LOCUS       DASSGX010000302         2995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1251359, whole genome shotgun sequence.
ACCESSION   DASSGX010000302 DASSGX010000000
VERSION     DASSGX010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251359"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VFI12_05305"
     CDS             <1..357
                     /locus_tag="VFI12_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055857.1"
                     /translation="SIGDYVLTGGELAAAVVIDCVTRLLPGVIKAESVAEESHAGGML
                     EYPHYTRPATFRGIAVPDVLLSGNHAAIRRWRHEQALLRTASTRPDLLAGLSPDDQRL
                     LANLVSGQDEHTQESQ"
     gene            441..1805
                     /locus_tag="VFI12_05310"
     CDS             441..1805
                     /locus_tag="VFI12_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7055858.1"
                     /translation="MSHVLTLTEFELDQYAFGEETSSHMATTGLMERAAAPEAATVAR
                     EPQSVEQTGLDLATIADLVLKFIYYNNQITAQSISDDICLPFYNVVDRSLMVLKKEEL
                     IEVAGSNGFGELAYQYSITPKGVVRVHQILERTTYVGPAPVTVDHYRRVIMAQAISTV
                     RVGPTEVREALSDLVLEDHVMDAMGQAVNTGRSLFLFGPPGNGKTMLAEHIVPLLGGE
                     VLIPHAFTVDGQIIKVLDLHNHVPVQVTGRPDFDRRFVLCKRPAIITGGELMLEGLDL
                     VYDKFARIYEAPLQLKANGGMLLIDDFGRQQVSPRQLLNRWIVPLEKRVDYLTLHTGK
                     KIEIPFDQLIVFSTNLQPKDLVDEAFLRRIQNKIHVTNPSVDIYREIFRRQCDALGVP
                     FDQNGLVYLLREYYVKPKRELRSVHPRDILRTLIGIARYHNISPVLSPKLLDRACQTY
                     FVDL"
     gene            complement(1787..2608)
                     /gene="recO"
                     /locus_tag="VFI12_05315"
     CDS             complement(1787..2608)
                     /gene="recO"
                     /locus_tag="VFI12_05315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HET7055859.1"
                     /translation="MDSAPASTHSSARDRIYRCDAIVLRRMDLGETDRILTLLSDRFG
                     KIRVIGKGIRRPTSRLGPHLELFSTTRLMLSRGRDLDVITAAETLDLHVGLRSDLDAL
                     GVASHCAELVDRFLADRDENRAVYRLMNTALERLDAGAAPPRVARWFEVALLGEMGFR
                     PELFNCVVCDVPVAAEPNRFSVRLGGILCHEHADRDLSAILLSIGAQKVLRLLIRGEL
                     ESFLGLAASDAVMSEVETVLTVFITHQLERDLNSLKVMRRVGESLPAWDDYRSTK"
     gene            2661..>2995
                     /locus_tag="VFI12_05320"
     CDS             2661..>2995
                     /locus_tag="VFI12_05320"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HET7055860.1"
                     /translation="MATIGQYPANCDAPVNSVSYTAAFVAGVLSITSPCVLPLIPLYG
                     AYLMGSTDRSRTRRGPLMINAAAFVLGFSLVFVLVGTAFGFLGTAMAERKTLLVQLGG
                     LLLIALGLH"
BASE COUNT          571 a          963 c          899 g          562 t
ORIGIN      
        1 tccatcggcg attacgtcct gaccggcggt gagttggccg cggcggtcgt gatcgactgc
       61 gtcacccgac tgctgccggg cgttatcaaa gcggagtcgg tcgccgagga gtcccatgcc
      121 ggcgggatgc tcgagtaccc gcattacacg cgccccgcca cgttccgggg catcgcggtg
      181 cccgatgttc tcctcagcgg caatcacgcg gcaatcaggc gctggcgaca tgaacaggcg
      241 ctgctcagga cggcctcgac tcgtccggac ctgctcgccg ggctatcacc agacgatcag
      301 cgcctgctcg ccaatttggt atccgggcag gacgaacata cacaggagtc gcaatagcgg
      361 acgggacttt ggcgctatgt gattcgggct atgccaggag tagcctgaaa atcaagaaga
      421 cgatatcgag cgcgggcact gtgtcccacg tcctgacgct caccgaattc gaacttgatc
      481 agtacgcatt tggtgaggag acctcatccc acatggcgac caccggactc atggagcgtg
      541 cggctgcccc cgaagccgca accgtcgcgc gtgaacctca atcggttgag cagaccggac
      601 tcgatcttgc cacgattgcg gatctggttc tgaaattcat ctactacaac aaccagatca
      661 ccgcgcaaag catctcggac gatatctgcc tccccttcta taacgtcgtc gaccgctcgt
      721 tgatggtgct gaagaaggaa gagttgatcg aggtcgccgg ttccaacgga ttcggtgaac
      781 ttgcctacca gtattcgatc accccgaagg gtgtcgtccg ggtgcaccag atcctggagc
      841 gcaccaccta cgtcggcccc gcgccggtga ctgtcgatca ctatcgccgg gtcatcatgg
      901 cccaggcgat ctcgacggtt cgagtcggcc cgaccgaggt ccgcgaggca ctgtccgacc
      961 tggtcctcga ggaccacgtc atggacgcga tgggccaggc ggtcaacacc ggccgctcgc
     1021 tcttcctgtt cggccctcct ggtaacggca agacgatgct ggcggagcac atcgtgccgc
     1081 tgctgggcgg tgaggtgctc atcccacatg ccttcaccgt cgatggccag atcatcaagg
     1141 tgctcgacct gcataaccac gtgccggtgc aggttaccgg ccggccggac ttcgatcgtc
     1201 gcttcgtgct ttgcaagcgt ccggcaatca tcaccggtgg cgagctcatg ctcgaagggc
     1261 tggacctcgt ctacgacaag ttcgcgcgca tctatgaagc gccgctgcag ctcaaggcca
     1321 atggcggcat gctgctgatc gacgacttcg gtcgccagca ggtctcaccg cgccagctgc
     1381 tcaaccgctg gatcgtcccc ctggaaaagc gggtcgacta ccttaccctc cacacgggca
     1441 agaagatcga aatcccgttc gatcagctca tcgtcttctc caccaacctg cagccgaagg
     1501 acctcgtcga cgaggcgttc ctgcgccgta tccagaacaa gatccacgtc acgaatccat
     1561 ccgtcgacat ctatcgcgaa atcttccggc ggcagtgtga cgcgctcggc gtgccattcg
     1621 atcagaatgg gctcgtctat ctgctgcgcg agtactacgt gaagccgaaa cgggagctcc
     1681 ggtcggtgca tccgcgcgat atcctgcgga ccctcatcgg cattgcccgg tatcacaaca
     1741 tctcaccggt gctctcgcca aaactgcttg atcgggcctg ccagacctac ttcgtcgatc
     1801 tgtagtcgtc ccacgcaggg agcgactcac cgacgcggcg catgactttg aggctgttca
     1861 ggtcgcgctc gagctggtgg gtaatgaaca ccgtcagtac cgtctcgacc tcgctcatca
     1921 cggcgtccga ggcggcaagt ccgaggaagc tttccagctc gccccggata agcagacgga
     1981 gcactttctg cgcgccgatc gagagcaaaa tcgcgctcaa gtcgcggtcc gcatgctcgt
     2041 gacaaaggat tccgccgagc cggacgctga agcgattcgg ctcggcggca accggcacat
     2101 cgcagacgac gcagttgaat aattccggcc ggaatcccat ttcgccgagc agcgcgacct
     2161 cgaaccagcg ggcgacccgt ggtggcgcgg cgccggcgtc caaccgctcg agcgcggtgt
     2221 tcatgagtcg atagaccgcg cggttctcgt cacgatcggc caggaaacga tcgaccagct
     2281 cagcgcaatg tgacgcgacg ccgagcgcat cgaggtccga ccgcaagccg acgtgcagat
     2341 cgagcgtctc tgccgccgtt atcacgtcca gatcccggcc gcgcgagagc atcagccgcg
     2401 tcgtcgagaa aagctcaaga tgcggcccca gccgggaagt agggcgacga atccccttgc
     2461 cgattacccg aatcttgccg aagcggtcgg agagcaacgt caggatgcga tccgtctcgc
     2521 cgagatccat gcggcgcagg acgatcgcgt cgcatcggta gatgcggtca cgggcgctgg
     2581 aatgtgtgga ggcgggtgcg gaatccatgc agtgagtgcc gtcgtagagc ccaacggatt
     2641 gtcccgatga ctgtaccatt gtggcgacga tcggccagta tcccgcgaac tgcgatgccc
     2701 cggtgaacag cgtttcctat acagctgcat ttgtcgccgg agtgctgtcc atcacgtcgc
     2761 cgtgcgtgct gccgctcata ccgctgtacg gtgcctacct gatggggtca accgaccgct
     2821 cgcggacgcg acgcggaccg ctcatgatca atgcagcagc gttcgtgctg ggattctcgc
     2881 tcgtcttcgt attggtcggc actgcgttcg gattccttgg cacggccatg gcggagcgaa
     2941 agacgctcct cgtccagctc ggcggtttgc tgctgatcgc cttggggctg catca
//
LOCUS       DASSGX010000303         3722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1251588, whole genome shotgun sequence.
ACCESSION   DASSGX010000303 DASSGX010000000
VERSION     DASSGX010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251588"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1030
                     /locus_tag="VFI12_05325"
     CDS             <1..1030
                     /locus_tag="VFI12_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055861.1"
                     /translation="ITFSGEQRSIPPDRAGVSGNQYIDQANRWAVTWDASALTAENWI
                     PSDSTSTQGVQLTSASGNFMTIFTDEATSLRACVSAQVDRFDGTAFSGFTPAEGVGPP
                     ATGKNARAALFQGVFTSQSGEHSAIYLSVECRPLVMAGQKVERHFLVATLISDVSTFA
                     SDVPAWSDVLRSVRFDAAGTTGISGDTYTSSIGYQLAWDDSVYVADYLDTANPDLGIS
                     LSSRDAILNIQVSGDPTADVCVSAEVDIVEGLDGMGTLRRSRVHGPEPGAGSASRMFD
                     SAVTFKNGTESAAVVYIECRPLGEVSDGPLFVVIRMVGIKSSYSDELPRWQDILDSVT
                     VIRPAGQD"
     gene            1112..2239
                     /locus_tag="VFI12_05330"
     CDS             1112..2239
                     /locus_tag="VFI12_05330"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HET7055862.1"
                     /translation="MAEPLSIDTLAVRGGYEPKHGDGISPKIDLSTTFHLAGDGDDGK
                     GTAYSRSDVPAYQVFERAIADVEGADHALVLNSGTSAMVALFDELTAGDRLVMTQDAY
                     FGFYIYARDTLGARGVTVDFIDFSNLAMVEQAISGAALAWVETPTNPHLHVTDLHAVA
                     GICAAHGVRWVADNTFSSPVLTRPIDLGAWAVMESVTKYLGGHSDVLLGAVASNDADL
                     IARAEHRRSQIGTQPDAFSCWLARRGMQTLALRVRRQSDTALRIAQELHAHPKVTRVH
                     YPGLEGDPGHEIAKRQMCGGFGGMLSFVVTGGKAAANRVTELTKVWVPATSLGSVESL
                     IERRSRWDGDDVVDPALLRLSAGIEHPDDLWNDLAQALEQI"
     gene            2471..>3722
                     /gene="proS"
                     /locus_tag="VFI12_05335"
     CDS             2471..>3722
                     /gene="proS"
                     /locus_tag="VFI12_05335"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HET7055863.1"
                     /translation="MRMSHMFGRTLREAPGDAEIISHKLMVRAGMIRSLGSGLFSMLP
                     LGYRTIKKIEQIIREEMDAIGGQEMLMPVVHPADIWKESGRWYAVGPELTRFTDRADR
                     DMVLAMTHEEIVTDIARQDINSYRQLPQIVYHLQTKWRDEPRARAGLIRVREFTMKDS
                     YSLDLDEAGLDVSYEMHREAYHKLFGRMGLQFMIVGADVGMMGGSKSEEYMAFSPNGE
                     DTILVCPSGDYAANREVARFIRDTPAPQEPLPIEEVATPNTPTIESLAKFLGIETKQT
                     AKAVFFEGGSGKFIFAVIRGDLDINETKLRHLVNETDLVPATAERIKAHGAEPGYGSP
                     VGVKDAVIVVDESIRDGANFVAGANKPGYHLKNVNLGRDYQAAVVGDIAEAKAGMLCP
                     ICGTPLTEERAIEIGNIFKLGSRYS"
BASE COUNT          682 a         1181 c         1199 g          660 t
ORIGIN      
        1 gatcacgttc tccggcgagc agcgatcgat accccctgac cgtgccggcg tgagcgggaa
       61 tcagtacatc gatcaggcga accgctgggc agtgacgtgg gacgcatccg cgctgaccgc
      121 cgagaactgg attccgtctg attcgaccag cacgcagggt gtgcagctta cctccgcgag
      181 cggcaatttc atgaccatct tcaccgatga agcgacatcc cttcgcgcct gcgtgtccgc
      241 ccaggtcgac cggtttgatg gcaccgcgtt cagtggcttc acaccggccg aaggtgttgg
      301 tccgccggca acgggaaaga atgctcgcgc cgcgctcttc cagggcgtct tcacaagcca
      361 gtcgggcgag cacagcgcaa tctacctttc tgtcgaatgc cgaccgctcg tcatggccgg
      421 ccagaaggtt gagcggcact tcctggtcgc aaccctgatc agtgatgttt cgacctttgc
      481 cagcgatgtg cctgcatggt cggacgtgct gcgatccgta cggttcgacg cggccggcac
      541 taccggcatt agcggcgata cgtatacctc cagcatcggt tatcaactcg cctgggatga
      601 ttctgtctac gtggcggact atctcgacac ggcgaacccg gacctcggca tcagtctgag
      661 ctcgcgcgac gcgatcctca atatccaggt gtccggcgat cccacggccg atgtatgcgt
      721 cagcgcagag gtcgacatcg tcgaagggct ggacggcatg ggcacactcc ggcggagtcg
      781 cgtgcacggt ccggaaccgg gcgccggctc tgcctcacgc atgttcgaca gtgctgtgac
      841 cttcaagaac ggcaccgagt cggccgccgt ggtctacatc gagtgccgcc cgcttggcga
      901 ggtaagtgac ggcccgttgt tcgtggtcat ccgcatggtc ggaatcaaga gctcctacag
      961 cgacgagctc ccgcgctggc aggacatcct cgactccgtc acggtcatcc gcccggccgg
     1021 ccaggactga cggcgacacc tcgccacgaa tccgcgccct ctatactcac ccggaccgaa
     1081 tccggaaata cgcgtcagac aggggacctt catggcagaa ccgttgtcca tcgatacgct
     1141 cgccgtgcgc ggcgggtacg agccaaagca cggtgatggg atttcgccga agatcgacct
     1201 gtcgacgacg ttccacctcg ccggcgatgg cgacgacggg aagggcacgg cctactcgcg
     1261 ctccgatgtc cccgcatatc aggtcttcga gcgggccatc gcggacgtcg aaggggcaga
     1321 ccatgcgctg gttctgaatt cgggcacgtc ggcgatggta gcgttgttcg acgagctcac
     1381 ggcgggcgat cggctcgtca tgacgcagga tgcctatttc ggcttttaca tttatgcccg
     1441 ggacaccctc ggcgcccggg gcgtcacagt cgatttcatc gatttctcca acctggcaat
     1501 ggttgagcaa gccatctccg gcgcggcgct cgcctgggtc gagacaccga ccaatccaca
     1561 cctccacgtc accgatctcc atgccgtggc cggtatctgc gcggcgcatg gcgtgcgctg
     1621 ggtggccgac aatacctttt cctcgccggt cctgacgcga cccatcgacc tcggggcatg
     1681 ggcggtgatg gagagcgtga ccaagtatct cggcggccat tcggacgtcc tgctcggtgc
     1741 ggtggcctct aatgatgcgg acctgatcgc ccgggcagag caccgccggt cacagatcgg
     1801 gacgcagccg gacgccttct cctgctggct ggcccggcgg ggcatgcaga ccctcgccct
     1861 tcgcgtccgc cgccagtcgg acacagcgct ccggatcgcg caggagttgc acgcgcatcc
     1921 gaaggtgacg cgcgtccatt atcccggcct ggagggcgac ccgggccatg agatcgcgaa
     1981 gcggcagatg tgcggcgggt tcggcggcat gctctcgttc gtcgtcacgg gcggcaaggc
     2041 agccgcgaac cgggtgaccg agctgacgaa ggtctgggtg ccggcgacga gcctcggcag
     2101 cgtcgagtcg ctgatcgagc gccgcagccg ctgggatggt gacgatgtcg tcgatccggc
     2161 cctgctccgt ctctcggccg gtatcgagca cccggacgac ctctggaacg acctggcgca
     2221 agcgctggag cagatctgag gacttcagtt ataggtggtt agtgattagt ggttagtgat
     2281 tggcgtgaat ccattcacta gccactaatc actcaccact aaccactgat gtccgatgtc
     2341 tgacgtccga cgacttgttc cggtggctaa atctgcgcgg cggttctatt ctcacgcgag
     2401 agattgatcg tttgggccgg tggcgtcccg tccaccggct gtgttttgcc acaagcaagg
     2461 accgatacca atgcggatgt cccacatgtt cggccgcacc ctgcgggaag ccccggggga
     2521 tgccgaaatc atcagccaca agctcatggt tcgggccggg atgatccgct cgctgggctc
     2581 cggcctgttc agcatgctgc cgctcgggta ccggacaatc aagaagatcg agcagatcat
     2641 ccgcgaggag atggacgcga tcggcgggca ggagatgctg atgccggtcg tccacccggc
     2701 cgacatctgg aaggaaagtg gccggtggta cgcggtcggg ccagagctga cgcgatttac
     2761 cgatcgggcg gaccgggaca tggtcctcgc gatgacgcac gaggagatcg tcacggacat
     2821 cgctcggcag gacatcaatt cctaccgcca gctcccgcag atcgtctacc acctgcagac
     2881 caagtggcgc gacgagccgc gggcccgggc aggcctgatc cgtgtccgcg agttcacgat
     2941 gaaggactca tacagcctcg acctcgatga ggccggactg gacgtctcgt atgagatgca
     3001 tcgcgaggcg taccacaagc tgttcggccg gatgggtctg cagttcatga tcgtcggcgc
     3061 ggacgtcggg atgatgggcg gctcgaagtc tgaggagtac atggccttct cgccgaacgg
     3121 cgaggacacg atcctggtct gcccgagcgg cgactacgcg gccaatcgcg aggtggcccg
     3181 gttcatccgc gatacgccgg cgccgcagga gccgctgccg atagaggagg tcgcgacgcc
     3241 gaatacgccg acgatcgaga gtctggcgaa gttcctcggc atcgagacga agcagacggc
     3301 caaggcggtc ttcttcgaag gcggatccgg caagttcatc ttcgccgtca tccggggcga
     3361 tcttgatatc aacgagacga agctccgcca cctggttaac gagaccgacc tcgtcccggc
     3421 cacggccgag cggatcaagg cccatggcgc ggaaccgggg tacggctcgc ctgtcggagt
     3481 aaaggacgcg gtgatcgtgg tcgacgagag catccgcgac ggcgcgaact tcgtcgccgg
     3541 ggcgaacaag cccggatacc acctcaaaaa cgtcaacctc ggccgagatt accaggcggc
     3601 tgtcgtcggc gatatcgcgg aggccaaggc gggcatgctc tgcccgatct gcggcacgcc
     3661 gctgacggaa gagcgagcga tcgagatcgg caacatcttc aagctcggct cccgctacag
     3721 cc
//
LOCUS       DASSGX010000304         4099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_55432, whole genome shotgun sequence.
ACCESSION   DASSGX010000304 DASSGX010000000
VERSION     DASSGX010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55432"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VFI12_05340"
     CDS             complement(<1..324)
                     /locus_tag="VFI12_05340"
                     /inference="COORDINATES: protein motif:HMM:NF020905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HET7055864.1"
                     /translation="MQALHGRVAIASRRITPGTAARFELRTDQLIQIVTLDGRQAAEM
                     IAFRADDSSERLSTAATRAKNKSIMIEAGKKLYSSRFAPMLELVTDTVGRHDLLMASS
                     EPVKPA"
     gene            460..1560
                     /gene="chrA"
                     /locus_tag="VFI12_05345"
     CDS             460..1560
                     /gene="chrA"
                     /locus_tag="VFI12_05345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HET7055865.1"
                     /translation="MTKLALRMGLTAFGGPAAHIAILRHEAVEKRQWLSERDFLDYLS
                     LANLIPGPNSTELVMHVGARVGGQRGLWAAGAAFIGPAAAITLTFAYVYKRWGSSPAA
                     EGILSGLAPALIVVIIFALVPLARTAIRSQWAVGLAAALVLLYVAGINELLLLLLGTL
                     AYVVPFALRALKDRTLAVAAPLLLFATAADQPDPTLVRIFLVFLKIGAVLYGSGYVLI
                     AFLQRDVVNHRHWITQQQLVDAVAVGQVTPGPVFSTATFIGYLVGGWGGAAAATLGIF
                     MPAFLLVGATHGLAARIRSSASLSHILDGLNLASNALLVGVLVTLIRDLDVSVVNQLV
                     GAIALAGLASKRVGPTTVLVAAALVGFLWHGL"
     gene            1713..3113
                     /locus_tag="VFI12_05350"
     CDS             1713..3113
                     /locus_tag="VFI12_05350"
                     /inference="COORDINATES: protein motif:HMM:NF017967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HET7055866.1"
                     /translation="MTPTRSPHARRSARPRFGPTLAFLALMLAIFGAAVTNGGLAAKK
                     ADTIVSIGESNSQDQRDELLGYFGADKNAKVDVITVAQTKDAMQDVIPDFNLSSAFSS
                     AALTCRPLGEGLDVTTINITQITPAMYAMALVTAGVGDAKLIVAAPAAAPAQGMTALT
                     GIFKSWDKVECESAQTTPARQQLALRELAVTVEISTAIGEVQTGYAGAFVIDTQRVIV
                     TNKYTTRDEITSAVQQMEATYGFAVPEPSRTKLIDLLVDLAKEKIDWSTFSAGWTIEY
                     PTATQIKMTGDGIAIRNAQASATAKAAKEQTAAAKAKTATARAEAEMTQTAIAADLTA
                     TALAQPTATATATPMPNEYSGTLTAPVTDNTLAVKFADGVEKSYQIAKTVAITRDGKA
                     ATADKLKKGDSVALKVDAMTDEVVDIVATAPAKSGTPFAKLIFLLPVLLLIPLGMVVK
                     GKSGGDPFVVKRVARD"
     gene            3132..3665
                     /locus_tag="VFI12_05355"
     CDS             3132..3665
                     /locus_tag="VFI12_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055867.1"
                     /translation="MSVLTARNVPKKEPKDLKPAYNQADAIPPELLVRRDNMVRYEKD
                     GPIYATDGKVGTLRKVVVDEASAEVTEIVVAVDGSDRCIVLPSDVVDRSAGAALFISL
                     NSVQFAERVASGPGYTRNHFARADLKALLHHKDGSRPMHARRSVTNVGQDFVETPLGS
                     PLDRLKPPMPAGSVAAD"
     gene            3855..>4099
                     /locus_tag="VFI12_05360"
     CDS             3855..>4099
                     /locus_tag="VFI12_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055868.1"
                     /translation="MNSVPPMAPERSPFATTSTVPPYAEQVTEEPKNTETAEESGFAT
                     WDTATTPEPEVEETPGRSWDAPPPQDTAVPVNEDMIAS"
BASE COUNT          739 a         1375 c         1289 g          696 t
ORIGIN      
        1 cgccggtttg accggctcgc tcgaggccat cagcaggtcg tgccggccga cggtatcggt
       61 cacgagctcg agcatcggcg cgaatcgcga ggagtagagc ttcttgccag cctcaatcat
      121 gatcgacttg ttcttggccc gcgtggctgc cgtcgaaagc cgctcagatg aatcgtcggc
      181 gcggaaggcg atcatctctg ccgcctggcg cccatcgagg gtcacgatct ggatcagctg
      241 atcggtccgc agctcgaatc tggccgcagt gcctggggta atccgccgcg aggcgatggc
      301 tacccgacca tgtaatgctt gcaccgatcg acctcccagt cgagctttca gtaatacaca
      361 accaggcaat tgtcgcagcc cgctcggctc tgattcaatt ccccggcagt ccgcattcta
      421 cgcgcgatcc ggaggaaccc tctgcctgtc ctgagcgaca tgaccaagct ggccctgcgg
      481 atggggttga cggccttcgg tgggccggct gcgcacatcg ccatcctccg gcatgaggcg
      541 gtcgagaagc ggcaatggtt gagcgagcgc gacttcctcg attacctgag cctcgccaac
      601 ctgatccccg gcccgaactc gaccgagctg gtgatgcacg tcggcgcgcg ggtcggcggg
      661 cagcgcggct tgtgggcagc cggggcggcc ttcatcggac cggcggcggc gatcacactg
      721 acattcgcct acgtctacaa gcgctgggga tcgtcgccgg cagctgaggg catcttgtcc
      781 gggcttgcgc cggcattaat cgtcgtgatc atcttcgccc tggtcccgct cgcgcgaacg
      841 gcgatcagga gccagtgggc agtcgggctt gcggccgcgc tcgtcctcct gtacgtggcc
      901 gggatcaacg aattgttgct cctgctcctc ggcacactcg cctacgtcgt cccgttcgcg
      961 cttcgagcgc tcaaggatcg aaccctggca gtcgccgctc cgttactgct cttcgcgacc
     1021 gcagccgacc agccggaccc gactctggtc cggattttcc tggtcttcct caagatcggc
     1081 gcggtgctct acgggtcggg gtacgtcctg atcgccttcc tgcagcggga cgtcgtcaac
     1141 catcggcact ggatcacgca gcaacaactg gtcgatgccg tcgcggtcgg gcaggtcacg
     1201 ccgggtccgg tcttctcgac cgcgaccttc atcggctacc tcgtcggtgg ctggggcggg
     1261 gcggccgcgg cgacgcttgg catcttcatg ccggccttcc tgctggtcgg cgcgacgcac
     1321 gggctggccg cccggatccg ctcgtccgcg tcactttcgc acatcctcga cgggctgaat
     1381 ctcgcctcga acgcgctcct ggtcggcgtc ctggtcaccc tgatccgcga cctcgacgtg
     1441 agcgtggtga accagctggt cggcgcgatc gcactggccg gtctggcctc gaagcgggtt
     1501 gggccgacga cggtcctggt cgcagcggcg ctcgtcggtt tcctctggca cgggttgtga
     1561 catccaaacg ggactaacag gttagaccgt aattccgcct gcgccggtag catcgaaggt
     1621 gcgatgtggt cggccgaacg agcctgtctg acggcgcgca tccgctgcat ttgtgggaca
     1681 gaaacggacc ctgcccagga gaccggctga agatgactcc gacccgatca ccacacgccc
     1741 gacgcagcgc ccggccgaga tttggcccga cactggcgtt cctcgcgctg atgctcgcca
     1801 tcttcggcgc ggccgtcacg aacggcggtt tggcggccaa gaaggctgac acgatcgtca
     1861 gcatcggcga gagcaattct caggatcagc gcgacgagct gctcggctac tttggcgccg
     1921 acaagaacgc caaggtcgac gtcatcacgg ttgcccagac caaggacgcg atgcaagacg
     1981 tcatcccgga cttcaacctg agctcggcct tttcgtccgc cgcgctgacc tgtcgcccgc
     2041 tcggcgaggg ccttgacgtc accacgatca acatcacgca aatcacaccg gccatgtatg
     2101 cgatggcatt ggtcaccgca ggcgtcggtg acgccaagct gatcgtagct gccccagccg
     2161 ccgctccggc gcagggcatg acggccctga ccggaatctt caagtcgtgg gacaaagtcg
     2221 agtgtgagag tgcccagaca acgccggcgc gacagcagct tgcgctgcgg gaactcgcgg
     2281 tcaccgtcga aatctcaacg gcgatcggcg aggttcagac cggatatgcc ggcgcgttcg
     2341 taatcgatac ccagcgcgtg atcgtcacca acaagtacac gacgcgcgac gagatcactt
     2401 cggctgtcca gcagatggaa gcaacctacg gattcgcagt gcccgagccc agccgtacca
     2461 agctgatcga tctcctggtc gacctggcca aggaaaagat cgactggtca accttctcgg
     2521 ccggttggac catcgagtac ccgaccgcga cccaaatcaa gatgaccggc gacggcatcg
     2581 cgatccgtaa cgcgcaggcc tccgccactg ccaaggcggc caaggagcag acggccgccg
     2641 ccaaggcgaa gaccgcgacc gcgcgtgccg aagccgagat gacccagacc gcgatcgccg
     2701 ccgacctgac cgcgacggcc ctcgcgcaac cgacggcgac cgcgaccgcc acgccgatgc
     2761 cgaacgagta cagcggcacc ctgaccgcgc cggtcacgga caacacgctc gccgtgaagt
     2821 tcgccgacgg cgtcgagaag agctatcaga tcgccaagac cgtcgcaatc acccgggacg
     2881 gcaaggcggc cacggccgac aagctgaaga agggcgattc agtcgcgctc aaggtcgacg
     2941 cgatgacgga cgaggtcgtc gacatcgttg ccaccgcgcc ggccaagagc ggcacgccgt
     3001 tcgccaaact catcttcctg ctgccggtcc tgctgctcat cccgctcggg atggtggtaa
     3061 agggcaaatc cggtggtgat ccgttcgtcg tcaagcgcgt cgcgcgcgac taaaccgcaa
     3121 ggagcgatgt aatgtcagtc ctgaccgcgc gaaacgtccc gaagaaggaa ccgaaggatc
     3181 tcaagccggc ctataaccag gccgatgcga ttccgccgga gctgctggtc cgtcgcgaca
     3241 acatggtccg ttacgagaag gacggcccga tctacgcgac cgatggcaag gtcggcacgc
     3301 tcaggaaggt cgtggtcgac gaggcgtcgg ccgaggtgac ggagattgtc gtcgccgtcg
     3361 acggcagcga ccggtgcatc gtcctgccgt cggacgtggt cgatcgatcg gccggcgcgg
     3421 cgctgttcat ctcgttgaac agcgtccagt ttgcggagcg ggttgcctcc ggtccgggct
     3481 acacccggaa ccactttgcc agggcggatc tcaaggcgct gctgcatcac aaggacggtt
     3541 cccggccgat gcacgcccgg cgctcggtca cgaatgtggg ccaggacttc gtcgaaacgc
     3601 cgctcggttc gccgctggat cgcctcaaac cgcccatgcc ggccggctcg gtcgcagccg
     3661 actgacggta cctgtccata cacctccgtg cgatactgaa tatgcgatgc gaacccgcgt
     3721 tatgggctcg cgccttcgct tgctcgctgg ttccattcag tggatgaggc aatcacatga
     3781 atcgttgtcc caattgcgcc gcccagaacc gcgaaggggc gaagttctgc acaagctgcg
     3841 gatttcgact gcccgtgaat tccgtcccgc cgatggcacc ggagcgatct ccgttcgcca
     3901 cgacctcaac cgtgccgccg tacgccgagc aggtaactga agagccgaag aacacggaaa
     3961 ccgccgagga atcgggtttc gccacctggg acacggccac gacgcccgag cctgaagtcg
     4021 aggaaacgcc tggccgctcg tgggatgcgc cgcctcccca ggacaccgcc gtgccggtca
     4081 acgaggatat gatcgcgtc
//
LOCUS       DASSGX010000305         6089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1035171, whole genome shotgun sequence.
ACCESSION   DASSGX010000305 DASSGX010000000
VERSION     DASSGX010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6089
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035171"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VFI12_05365"
     CDS             <1..232
                     /locus_tag="VFI12_05365"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HET7055869.1"
                     /translation="GGKQYDVVIGNIIARVLISIADDLRAATKPGGLLLLSGIIEPKE
                     IETRERFDALGLDLLGRNQIEDWISLAYRAPA"
     gene            310..1734
                     /locus_tag="VFI12_05370"
     CDS             310..1734
                     /locus_tag="VFI12_05370"
                     /inference="COORDINATES: protein motif:HMM:NF016354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HET7055870.1"
                     /translation="MPVISPISLQRVDLAPTVPGVIEAIEPGSLAAQIGLRVGDRVVT
                     VNGTQVLDALDFQFHAQSQSVRIEILRDEKRRRFDLKLAGDEFWGITFADPTFDGIRV
                     CENACPFCFIKQIPKGMRRSLYVMDDDYRYSMLYGSFVTLTNLSDRDWARIEEQRVSP
                     MHVSVHATDPELRVALVGNPKGAQIMDDLERLRRNNIDFHAQLVLSPGVNDGEEMDRS
                     VRELAALDNCLSIAAVPVGLTRHGMERQSKQVRLSRTCMRLLPGKQITVRRYEPEEAR
                     AVIRQAEEWQRRFRAERGETFLHLGDEFYLMCGEPVPPAELYDGYPQIEDGIGITRHF
                     LENLQTYLGRAKAGSLDGAAATIACGTLIGETMHGAIDDLNRHLGSAVDVRILENTFF
                     GPEINISGLLTGKDLIAGFANESGTAPIYISSRMISDRTQTLLDDLTMEQVQAELGRP
                     VVPCLTLSDVARDLRHRQRAARAA"
     gene            1765..2031
                     /locus_tag="VFI12_05375"
     CDS             1765..2031
                     /locus_tag="VFI12_05375"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HET7055871.1"
                     /translation="MLTVRVHPRSSKNRHLVDADQILHVWVTAPAVEGAANMAVNRYL
                     ADVLEISRSRISLVSGETARTKRVRVDLPASSVKQRLNPDQRGT"
     gene            complement(2090..3922)
                     /locus_tag="VFI12_05380"
     CDS             complement(2090..3922)
                     /locus_tag="VFI12_05380"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HET7055872.1"
                     /translation="MDLLLGLGNRLVLLLTAVLLVGVLGLVAVRAIYSGKVLPSVYVA
                     DVPVGGLSKDEAYAAVQQRASTLINETFVFDYDGRQWTTTLADLGVQPDVQRSVDTAF
                     AVGREQEARDRVGNTISLATGNQTIPLALRLDSGKVNSWVETVTGDIDRSPQDAQIQI
                     TNGKVSVSKDVDGIVVDKDRLMAVISESIRTMAPYRGPLPIKLSAPSIRENDLDPAVA
                     EINSVLSNPVKIVYKDKQWMLQPADLGTFLVQTPNIGGTGVTVSVDSDALGQWLYNLV
                     GDRINRAPVNAEIKWNDAKNKVVATSGSSKGVTLLAGPLADSVVESMTGNHGDVDVPV
                     KGVKPEIDSDHLDDLGITTKLGVGTSSFYGSDENRATNIWVGTGHLNGAIVRPGQTFS
                     FNNAIGDITPEAGYVEASVVDGERIGKDVGGGICQVSTTVFRAAFLAGFPIGEWWPHL
                     YRLAFYEYDGWTPGLDASILQEGPRANWGDFTFENPTNSYLLVEAYVDGQTDVVTIYG
                     PETGWDVFVSDPWEGKPILGDDQPDVETVDSDLPPGTVMQSEYRQDGLEISYERIVKD
                     ADGKVIDDWIAYSRFAARGDVWKVSPDMKGQSPASLNPHKVSSN"
     gene            complement(4081..4536)
                     /locus_tag="VFI12_05385"
     CDS             complement(4081..4536)
                     /locus_tag="VFI12_05385"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET7055873.1"
                     /translation="MIADIALPIPYDWFLLATRVLIAAILLGFLWRVLIVVGREALLT
                     APAGGQLSLALLDEFDQPVRGFRLSRRKPMTIGRDPSNDIVLADRSVSGNHAVIRYLG
                     GEWVLADLDSRNGTYVNGAAIATETSISEHDVVQFGAVRLQMLTHESSI"
     gene            complement(4561..5313)
                     /locus_tag="VFI12_05390"
     CDS             complement(4561..5313)
                     /locus_tag="VFI12_05390"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HET7055874.1"
                     /translation="MVSSILDRFEESFERLVEGSVGRVFKTPIQPAEIGRKLERAMVT
                     NQVVSVDSTIVPNDYRVVLNPQDMIAFADLLPGLGGQMESWLSEVADLHGFRLIDRMR
                     VLIVGDESVPRRAIQVTAAIADKPGVNQRQQEELQRTELYRIVAKANGVQPLRFKITN
                     GPQAGAEFLLRKSVTTLGRALDNDIVLESSEVSRQHARIELQSDGMRIIDLNSTNGTR
                     VNGRSIRSQNVKAGDDVAFGNLSAKILGDVES"
     gene            complement(5432..>6089)
                     /locus_tag="VFI12_05395"
     CDS             complement(5432..>6089)
                     /locus_tag="VFI12_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055875.1"
                     /translation="LFLFSIPIWWVFEAANSRLDNWVYILPHRYSWLAYHAEASLAFS
                     TVAPAIFVMAEFVRTVLFRGQVRWIRIAPDRNGLLAISASGALLFIATMIWPNVLFPM
                     VWISLFFAIDPIVRLFGGRSVSGWVAEARWDPVVVLFTGALICGCFWEFWNFWSMPKW
                     TYRIAFADWSRVFEMPVLGYGGYLPFALEVFAFVALADRVLGSGIMAAVRFDRANNAE
                     "
BASE COUNT         1144 a         1939 c         1904 g         1102 t
ORIGIN      
        1 cggtggcaag cagtacgacg tcgtcatcgg gaacatcatt gcccgcgtgc tgatctcgat
       61 cgcggacgat ctccgagccg ccacgaagcc gggcgggttg ctgcttttga gcgggatcat
      121 cgagccgaag gagatcgaga cgcgcgagcg gtttgacgcg ctcggccttg acctgctcgg
      181 gcggaaccag atcgaggact ggatttcgct ggcctaccgc gccccggcct gattcggaac
      241 ccgtggtaaa atgctccgtt ggccgccgtc gggcggtcgc cgcgctttgc gaaagttccc
      301 gccaccgata tgccagtcat cagtcccatt tccctgcagc gagttgacct tgcgccaacg
      361 gtgccgggtg ttatcgaggc gatcgagccc ggttcattgg ccgcgcagat cggtctccgg
      421 gtcggcgacc gcgtcgtgac cgtcaacggg acccaggtcc tcgacgcgct cgacttccag
      481 ttccacgcgc aatcgcaatc ggtccggatc gagatcctgc gggacgaaaa gcgccgccga
      541 ttcgacctca aactggccgg tgacgagttc tggggcatca ccttcgcgga tccgaccttt
      601 gatgggattc gcgtctgcga gaacgcctgt cccttctgct tcatcaagca aatcccgaag
      661 ggcatgcgtc gctccctcta cgtgatggac gacgactacc gctactcgat gctctatggc
      721 tccttcgtca ccctgacgaa cctgtccgac cgcgactggg cccgaattga ggagcagcgg
      781 gtcagcccga tgcacgtgtc ggtgcatgcg accgatccgg agcttcgggt tgccctggtt
      841 ggcaatccca agggcgcgca gatcatggac gatctcgaac ggctgcggcg gaacaatatc
      901 gatttccacg cccaactcgt cctcagcccc ggtgtcaatg acggcgagga gatggaccgc
      961 agcgtccggg agctggccgc gctcgacaat tgcctctcga tcgctgcggt gccggtcggg
     1021 ctgacacggc acgggatgga gcggcagagc aagcaggtgc gactgtcgcg gacatgcatg
     1081 cggcttctgc cgggcaagca gatcacggtt cgccgctatg agccggaaga ggcgagagcg
     1141 gtcatccggc aggctgagga gtggcagcgt cggttccggg cggagcgcgg cgaaaccttc
     1201 ctccacctcg gcgacgagtt ctacctgatg tgcggcgagc cggttccgcc agcggagctg
     1261 tatgacggct acccgcagat cgaggacggc attggcatca cccgccattt cctcgagaat
     1321 ctgcagacat acctcggccg ggcaaaggcc ggcagcctcg acggcgccgc ggcgacgatc
     1381 gcctgcggca cgctgatcgg cgaaacgatg cacggcgcga tcgacgacct caaccggcat
     1441 ctcggctcgg cagttgacgt tcgcatcctc gagaacacgt ttttcggtcc ggaaatcaat
     1501 atctcgggcc tgctgacggg taaggacctg atcgcgggat tcgcgaatga gtcgggcact
     1561 gccccgatct acatctcgtc ccggatgatc tctgaccgga cgcagacact cctcgacgac
     1621 ctgaccatgg aacaggtcca ggccgaactc ggccggccgg tagtgccctg cctgacgctg
     1681 tccgacgtgg cccgggacct ccggcatcgc cagcgcgccg cccgggccgc ctgacgagcg
     1741 atgacagcag gcgatgagtc gacggtgctc accgtacgcg tccacccgcg ctcaagcaag
     1801 aaccggcacc tggttgacgc tgaccagatc ttgcacgtat gggtcaccgc gccggcggtg
     1861 gagggtgcgg cgaacatggc cgtgaatcgc tatctcgccg acgtgctcga aatctctcgg
     1921 tcgcgcatct cactcgtgag tggcgagacg gcgcgcacga agcgggtccg ggtcgatcta
     1981 ccggcgagtt cggtcaagca gcgactcaat cccgaccaac gaggaacgta atcgggccac
     2041 tcagtacgag cggcccgttc gtgaattcag gattatcggc gagcgccggt tagttgctgc
     2101 tgaccttgtg cggattgagg ctggccggag actggccttt catatcgggg ctgaccttcc
     2161 agacgtctcc acgggcagcg aagcggctat aggcgatcca gtcatcgatc accttgccgt
     2221 cagcgtcctt gacgatccgc tcgtaggaga tctcgagacc gtcctgccga tactcgctct
     2281 gcatcaccgt gcccggcggc aggtcagagt cgacggtttc gacgtccggc tggtcatcgc
     2341 cgaggattgg cttcccttcc catgggtcgc tgacaaagac gtcccagccg gtttccgggc
     2401 cgtagatcgt gaccacgtcc gtctgcccgt cgacatacgc ctcgaccaag aggtagctgt
     2461 tcgtgggatt ctcgaaagtg aagtcacccc agttcgcgcg gggtccttcc tggagaattg
     2521 acgcgtcgag accgggcgtc cagccgtcgt actcatagaa ggccagccgg taaaggtgcg
     2581 gccaccactc gccgatcgga aaaccggcca ggaacgcggc ccggaagacg gtcgtcgaaa
     2641 cctggcatat gccgccgccg acatccttgc cgatccgctc gccgtcgacc acggaggcct
     2701 cgacgtagcc ggcttccggt gtgatgtcgc cgatggcatt gttgaacgag aaggtctgtc
     2761 ccgggcggac gatcgcgccg ttgaggtggc cggttccgac ccagatattg gtcgcccggt
     2821 tctcgtccga gccatagaac gaggatgtcc cgacgccgag cttggtcgtg atgccgagat
     2881 cgtccagatg gtcactgtcg atctccggct tgacgccctt gaccggcaca tccacgtcgc
     2941 cgtggttgcc cgtcatgctc tcgacgacag agtctgccag cggaccggcc agcaacgtga
     3001 cgcccttgga gctcccggat gtcgcgacga ccttgttctt ggcgtcattc cacttgatct
     3061 cggcgttgac cggtgcgcga ttgatgcgat caccaaccag gttgtacagc cactgtccaa
     3121 gggcatcgct gtcgacggaa accgtcacgc ccgtaccgcc gatattgggg gtctggacga
     3181 ggaacgttcc gaggtcggcc ggctgcagca tccactgctt gtctttgtag acgatcttga
     3241 ccgggttgct caggaccgag ttgatctccg caactgccgg gtccaggtcg ttttcgcgga
     3301 tcgacggcgc gctcagcttg atcgggaggg ggccacgata gggcgccatg gtccggatcg
     3361 actccgagat gacggccatc agccgatcct tgtcgaccac gatgccgtcg acgtccttgg
     3421 agacgctgac cttgccgttg gtgatctgga tttgcgcgtc ttgcgggctg cgatcgatat
     3481 cgccggtgac ggtctcgacc caggaattga ccttgccgga gtccaggcgc agtgcgagcg
     3541 ggatcgtctg gtttccggta gcgaggctga tcgtgttgcc gacgcgatcg cgcgcttcct
     3601 gctcccggcc gacggcgaag gccgtgtcga ccgagcgctg cacgtccggc tgcacgccga
     3661 ggtcggcgag cgtggttgtc cattggcgac catcgtagtc gaagacgaag gtctcgttga
     3721 tcagcgtcga cgcgcgctgc tgcaccgctg cgtacgcctc gtccttgctc aatccgccga
     3781 cgggcacatc ggccacgtac accgacggca agacctttcc gctgtaaatc gcgcgcactg
     3841 ccacgaggcc gagcacaccg acgagcagaa cagccgtcag cagcaggacc aggcggttgc
     3901 caaggcccag cagcagatcc aattttgatt ttccggtgcg tgcgggcgcg gaagcggcct
     3961 ccgcggttcg ttgttcgcct gacagcgaat cggaaagcct ggctatcgct tcagccacgt
     4021 tttcgagtcc ccagccgttg gtgtaccacg tgtcgtatcc gcatggtggt acgcccgaac
     4081 tcagatcgaa gattcatgcg tcaacatctg gaggcgaacc gctccgaatt gtacgacgtc
     4141 atgctccgaa atgcttgtct cggtggcgat tgccgcgccg tttacgtacg ttccgttccg
     4201 gctgtcgagg tcggcaagta cccattcccc gcccaggtac cggatcacgg cgtggttgcc
     4261 ggaaacggag cgatcggcga gaacaatgtc attggagggg tcccgaccga tggtcattgg
     4321 tttccggcgc gagagccgga agccacgtac gggctgatcg aactcatcga gcagggccag
     4381 tgagagctgc ccgcccgcgg gagcggtgag caacgcctcg cggccaacca caatcaagac
     4441 gcgccagagg aacccgagca ggatggccgc gatcaggacg cgcgtcgcga gcaggaacca
     4501 atcgtatggg atcggcaggg cgatatcggc gatcacgatc cgtcgccggt gggggagtcg
     4561 tcaggactcg acgtcgccca ggatctttgc actgaggttg ccgaaggcga catcatctcc
     4621 ggccttgacg ttttgcgatc gaatgctgcg tccattgacc cgcgttccgt tcgtgctgtt
     4681 caggtcaatt attcgcatgc cgtcgctctg gagttcaatg cgcgcgtgct ggcgtgatac
     4741 ctcgctcgac tcgagcacga tgtcattgtc cagcgcgcgg ccgagggtag taaccgattt
     4801 acggagcagg aactcggcgc cggcctgcgg tccattcgtg atcttgaagc gaagcggctg
     4861 aacgccgttg gccttggcca cgatccgata aagctcggtc cgctgcagct cctcttgctg
     4921 gcgctggttg acgcccggct tgtcagcgat tgcggccgtg acctggatcg cccggcgcgg
     4981 cacagactcg tcgccgacga tgaggacgcg catccggtca atcaggcgga acccgtgcag
     5041 atcggcgacc tcgctcagcc acgattccat ctgtccgccg aggcctggca gcaggtccgc
     5101 aaacgcgatc atgtcctgcg ggttgagcac cacacggtag tcattcggaa cgatagtcga
     5161 gtcaacggag acgacctggt tcgtgaccat cgcgcgctcg agtttgcggc cgatttcagc
     5221 cggctggatc ggggtcttga atacccgacc gacggaccct tcgaccagcc gttcgaacga
     5281 ctcttcaaat cgatccagaa tgctcgatac cattccgcgc agacgcctcc cggcggtgat
     5341 tcggccaaaa cgaccgttca tgcattatag gaacggcgct ccgcgcgctc aatccgaaac
     5401 gagcccgcgg gcataggacc tttgaacaga atcactccgc attatttgcc cgatcgaagc
     5461 gcaccgccgc catgatcccg gatccaagca cgcgatccgc cagcgccacg aacgcaaaca
     5521 cctccagggc gaacgggaga tacccaccat atcccagaac cggcatctcg aaaacccggg
     5581 accagtccgc gaatgcgatc cgatacgtcc acttcggcat ggaccagaaa ttccagaact
     5641 cccagaagca cccacagatc aacgcgcccg tgaagagcac gacgacggga tcccagcgcg
     5701 cctcggcaac ccacccactg actgagcggc ccccgaagag ccggacgatc ggatcgatcg
     5761 cgaagaagag gctgatccag accatcggaa acaggacatt cggccaaatc atggtcgcga
     5821 tgaaaagcag tgcgccggac gcgctgatcg ccagcagacc gttccggtcc ggcgcgatcc
     5881 ggatccagcg cacctgcccc cggaacagaa ccgtccgaac gaattccgcc atgacgaaga
     5941 tcgccggcgc cacggtcgag aacgccaggc ttgcctcggc gtgataggcc agccacgaat
     6001 agcggtgcgg aaggatgtaa acccagttgt cgaggcggct gttggccgct tcgaacaccc
     6061 accagattgg tatggagaac aggaagagc
//
LOCUS       DASSGX010000306         2372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_383084, whole genome shotgun sequence.
ACCESSION   DASSGX010000306 DASSGX010000000
VERSION     DASSGX010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383084"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="VFI12_05400"
     CDS             <1..764
                     /locus_tag="VFI12_05400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET7055876.1"
                     /translation="VPEYYDEVWRDASMLHCCSEALWQRCRERGCPPETSHLVIPGGV
                     DLDFFDPGKRPQPGIAGDRERPLRILSVGRLVWTKGHDLAIGALRILVELGIDATLTI
                     VGGGAMVDELRFAAWDLGVFDRVHLAGSLPRRRVRAAMKQADVFLAASYSEGFGISVL
                     EAQAMQLPVVCTDADGLKENVVDGKTGFVVPRREAAPLADRLALLARAPKLRASMARA
                     GRERVAQHFRIDQDIDAFEWLYYDAIEANRCAGQA"
     gene            943..>2372
                     /locus_tag="VFI12_05405"
     CDS             943..>2372
                     /locus_tag="VFI12_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055877.1"
                     /translation="MLAYILLASRLGRPVASADVAKCTRYDMAELAFEPDQYLGWHSK
                     DRRLYLFAWQAFAEQGQMGSHWHIDRDEAVLFSGMPVPLDSPWTPGVGWADQLADKIA
                     ASDANTVAAELGGAFTLLHLQRDADSIVTNDLLGGAPIYWDASQELLVISNRANLVAS
                     VWEQPGTNSVRDWRGPTWSVFAGEPVDGDTGLKDVAALAGGEWWNLGWKKRPEQRTRP
                     LPPAPETNPVDRIEAALRRSLRAIAALPFQQCTLALDGGNASRLLLALAAAEGLLDRI
                     GLTATGDPDEAGVQIAVELADRVGRPLLLQTRSLTAPDEFSQQARIASFQRGGVGSPW
                     ELGGLLGSTDLVCIESDLAGALAPDARRRALLSGAPVFDPLGVLRPAISAFFADRIAA
                     RLGEDGDPKELMALAANPRSIAGSVETPVIGHAYPYLDPAIWSALSALSNEALLGESA
                     YTAIMERVAPKLLEVAVCDDLCASDGR"
BASE COUNT          385 a          786 c          795 g          406 t
ORIGIN      
        1 aggtaccgga gtattacgac gaggtttggc gcgacgccag catgctgcat tgctgcagcg
       61 aagccctctg gcagcgctgc cgggagcggg gctgcccgcc ggaaacatca caccttgtta
      121 ttccaggtgg ggtcgatctc gacttcttcg atcctggcaa acgcccgcaa cccggaatcg
      181 ccggcgatcg ggagcggcca ctgcgcattc tgagtgtcgg acggctcgtt tggaccaaag
      241 gacacgatct ggcgatcggg gcgctgcgca tactggtcga actggggatc gacgcgaccc
      301 tcaccatcgt cggcggtggc gcgatggtcg acgagctgcg attcgccgcc tgggatctcg
      361 gtgttttcga ccgcgtgcat ctggccggta gtctgccccg ccgccgcgtg cgcgccgcca
      421 tgaaacaggc cgatgtcttt ctggcggcca gctacagcga ggggttcggc atctcggtgc
      481 tggaagcaca ggccatgcag ctgccggtgg tctgcaccga cgccgacggt ctgaaagaga
      541 acgtggtcga cggcaaaacc ggcttcgtgg tgccgcggcg ggaggcagcg ccgctggccg
      601 accgcctggc gttactggca cgcgcgccca agctgcgggc cagcatggcg cgcgccggcc
      661 gcgagcgggt tgcgcaacat ttccgcatcg accaggacat cgatgccttc gaatggctct
      721 actacgacgc catcgaagcc aaccgctgcg cgggccaggc gtgaggcggt tgccagtccc
      781 cgtgcccgga gaatccgccg cagcgtgact gccggtcaac agggcatact atcgccggct
      841 cgcgcatgtc cgttcggtat gcccgagatg aaacgaacca cggtcgagcg caatcgcgcg
      901 accggcaaca ctggctgacc cgtcgagagg gacgacttcg gtatgctggc ctacattctt
      961 cttgcgagtc gtcttgggcg gccggttgca tcggccgatg tcgccaaatg cacccgttat
     1021 gacatggccg agctcgcttt cgagcccgac cagtatctcg gctggcatag caaagaccgg
     1081 cgactctatc tcttcgcctg gcaggcattt gccgaacaag gccagatggg ttcgcactgg
     1141 catatcgatc gcgacgaggc ggtgctcttc agcggtatgc cggtgccgct cgattctcca
     1201 tggaccccgg gtgtgggttg ggccgaccaa ctggcggaca agatcgccgc atccgatgcc
     1261 aacacggtcg cggccgagct gggcggcgca ttcacgctgc tccatctgca acgcgacgcc
     1321 gacagcatcg tcaccaacga tctgctcggc ggcgcgccga tctattggga cgcgtcgcag
     1381 gagttgttgg tcatcagcaa ccgcgcgaat ctggtcgcct cggtctggga gcagcccggc
     1441 accaactccg tgcgcgattg gcgcggtccc acctggtccg ttttcgccgg cgagccggtc
     1501 gatggcgata ccgggctgaa ggatgtggcg gcgctcgccg gcggggaatg gtggaatctc
     1561 ggctggaaga aacgccccga gcagcgcacg cgcccactcc ctcccgctcc agaaacgaat
     1621 ccggtcgacc ggatcgaagc ggcgctgcgt cgctcactgc gcgctatcgc agccctgccc
     1681 tttcagcaat gcaccctggc gctggatggc gggaacgcca gccggctgtt gctggcgctg
     1741 gccgccgcgg aagggttgct cgaccggatc gggctgaccg ccaccggcga tccggacgaa
     1801 gcaggcgtgc agatcgcagt cgagctggcc gaccgggttg ggcgcccgct gcttctgcaa
     1861 acccgctcgc tcaccgcgcc ggacgaattt tcgcaacagg cccgcatcgc ctcctttcag
     1921 cgaggcggcg tgggcagtcc atgggagctc ggcggcctgc tcggttcgac cgatctggta
     1981 tgcatcgaat ccgatctggc cggggcgctg gcgcccgacg cgcgccggcg cgcgttgctc
     2041 agcggcgcgc cggtattcga tccgcttggg gtgctgcgtc ccgcgatttc ggcgttcttc
     2101 gccgaccgca tcgccgcccg cctcggcgaa gacggcgatc ccaaggagct gatggcgctg
     2161 gcagccaatc cacgctcgat cgcgggatcg gtcgaaacac cggtcatcgg gcacgcctat
     2221 ccctatctcg acccagccat ctggagtgcg ctgagcgcgc tgtccaacga agcgctgctg
     2281 ggggaatcgg cgtacaccgc catcatggaa cgggtcgcac cgaagctgct cgaggtggcc
     2341 gtctgcgacg atctttgcgc aagcgacggc cg
//
LOCUS       DASSGX010000307         2296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_273351, whole genome shotgun sequence.
ACCESSION   DASSGX010000307 DASSGX010000000
VERSION     DASSGX010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273351"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="VFI12_05410"
     CDS             complement(<1..886)
                     /locus_tag="VFI12_05410"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET7055878.1"
                     /translation="MTTPAFSRRSVVKKGAALAGLSMMQVSGPVRAFQGDSGGEVIPW
                     LDKLAENPVPENIVIQMDWAKLDSWITPMDQFFVIKHFNLPELNEKDWSLAISGQVAK
                     PLTFSLDDIKARARREVEFTIECSGNTGLPFFNGGVGNAKWGGAPLAPLLREAGVLEP
                     GIEVVFWGADAGEHKYGDVTVSEHLARSMSLADALEGDNILAYEMNGDPLPPLHGYPL
                     RLIAPNWYGIANVKWLTRIEVLDHRYAGFFMAQEYVSISQQERDGEDVWTFTSVGRKR
                     LKSAPAKVSKSGNSYSIMG"
     gene            complement(1310..>2296)
                     /locus_tag="VFI12_05415"
     CDS             complement(1310..>2296)
                     /locus_tag="VFI12_05415"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HET7055879.1"
                     /translation="ISYKEAMDKYGTDRPDIRFDLTFQDVTSKFGQTGFNAFAGVVTS
                     GGQIKAIIVPDAADYTRREIDELTETAKRAGAKGLATVAMQEGGVKSPIAKFLSEAEI
                     AALTTDIGAKTGDLVLIVADTAAVVAKALSTLREELGQRLGLADPNVLAYCWVVDFPL
                     LEWDEENQRWDSPHNPFCGFIEEDRPKLDTDPGNIRSKQYDLVLNGNEVGGGSVRNHR
                     REDQAKIFTLMGHSPEAQQDRFGAILTALEYGAPPHGGIAMGIDRYVMLLADEVNIRD
                     VIAFPKNQRGVDLMFDAPSTVDEQQLCDVGLELLPEVREQVAAEAEIVKGPR"
BASE COUNT          397 a          735 c          710 g          454 t
ORIGIN      
        1 cgcccatgat gctgtagctg ttgccggatt tcgacacctt ggcgggcgcc gacttgagtc
       61 gtttccggcc gacggacgta aaggtccaga cgtcttcgcc gtcacgttcc tgctgcgaga
      121 ttgagacgta ttcctgcgcc atgaagaaac cagcgtagcg gtggtccagc acctcgatcc
      181 gagtcagcca cttgacgttc gcaatgccgt accaattggg cgcgatcagc cgcagcggat
      241 acccgtgcag cggcgggagc ggatctccat tcatctcgta ggcgaggata ttgtcgccct
      301 caagtgcgtc tgccagggac atacttcggg caaggtgctc ggaaaccgta acatccccgt
      361 acttgtgctc gccggcgtct gcgccccaga agacgacctc aatgccgggc tcgagcacgc
      421 ccgcttcccg gagcagcggc gcgagcggcg cgccgcccca tttcgcgtta cccacgccgc
      481 cattgaagaa tggcagcccg gtattgccgg agcactcgat ggtgaactcc acctcccggc
      541 gcgctcgggc tttgatgtcg tccagcgaga aggtcaaggg cttcgccacc tggccgctga
      601 tcgccaggga ccagtccttc tcgttcagct cgggcaggtt gaagtgcttg atgacgaaga
      661 actggtccat cggggtgatc caggaatcca gtttggccca atccatctga atgacgatat
      721 tctccggaac cgggttttcc gccagtttgt ccagccaggg gatgacctcc ccgcctgagt
      781 caccctggaa cgcacgcaca ggtccagaca cctgcatcat gctcagaccc gccagagccg
      841 cacccttctt taccactgat ctgcgggaaa acgcgggagt cgtcattgcc cttctcctta
      901 ttccgctgaa cgcaaacgtc gccgatggtg cccattgtct gaggcaagaa ctcgcgactg
      961 ctgctcaaga caccgaggat cccggtaatc tcggaaactg cgtcgtaact ggggtcacaa
     1021 ttcgatcccg tttgcggctg cgaccgggca ttatcccgtt agcgaccaag tggtcagtcg
     1081 atagcccgtg gtggtcatgg agagtgcaca gtcgccgagc cggaccgtcc gctcgttgac
     1141 cccgagcggt tcacctgcgt ttcgggcgca gtgcggctag ccggcggcag ttccggtcgt
     1201 gccggatgcc gcgatgtccg gcgtctgccc atttatgggt cctcccagaa tgggcgaccg
     1261 ccggaccgat tgcaatgctt agcgtaataa tacctagtca agtgtacgtt cagcggggtc
     1321 ccttgacgat ttcggcttcc gccgcgacct gctcgcggac ctccggcagc agctcgagac
     1381 cgacgtcgca gagctgctgc tcatcgaccg tcgagggcgc gtcgaacatc aggtcgacgc
     1441 cgcgctggtt cttcgggaag gcgatcacgt cgcggatgtt gacctcgtcg gcgagcaaca
     1501 tcacatagcg gtcgatgccc atcgcgatgc caccgtgtgg tggtgcgccg tactcgagcg
     1561 cggtcaggat cgcgccgaag cggtcctgct gcgcttcagg cgagtggccc atcagcgtga
     1621 agatcttggc ctgatcctcg cggcgatggt tccgaaccga gccgccgccg acctcgttgc
     1681 cgttcagcac gagatcgtac tgcttcgagc ggatgttgcc gggatcggtg tcgagcttcg
     1741 gccgatcctc ctcgatgaag ccgcaaaagg ggttgtgcgg cgaatcccag cgttgattct
     1801 cctcgtccca ctcgagcagc gggaaatcga cgacccagca ataggcaagc acgttcggat
     1861 cagccaggcc gagccgctgg ccgagctcct cgcgcagggt cgagagcgcc ttggcgacga
     1921 ctgccgccgt gtcggcgacg atcaggacga ggtcgccggt cttcgcgccg atgtccgtgg
     1981 tcagggcggc gatctcagct tcggagagga acttggcgat cggcgacttg acgccgccct
     2041 cctgcatcgc gacggtcgcg aggcccttgg ccccggcccg cttggccgtt tcggtcaatt
     2101 cgtcgatctc gcggcgggtg taatcggccg catccgggac aatgatcgcc ttgatctggc
     2161 ctccactcgt gacgacgccg gcgaaggcgt tgaacccggt ctgcccgaac ttgctcgtca
     2221 cgtcctggaa tgtcaggtcg aagcggatat ccgggcggtc cgtgccgtac ttgtccatcg
     2281 cctccttgta ggagat
//
LOCUS       DASSGX010000308         5100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_708313, whole genome shotgun sequence.
ACCESSION   DASSGX010000308 DASSGX010000000
VERSION     DASSGX010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5100
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708313"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..353
                     /gene="infA"
                     /locus_tag="VFI12_05420"
     CDS             153..353
                     /gene="infA"
                     /locus_tag="VFI12_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003808114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HET7055880.1"
                     /translation="MTVDGKVVDALPNAMFRVELENGHTVLSHLAGKMRTNYIRVLPG
                     DRVTVELSPYDLTRGRITYRHR"
     gene            514..1584
                     /locus_tag="VFI12_05425"
     CDS             514..1584
                     /locus_tag="VFI12_05425"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HET7055881.1"
                     /translation="MRSWRFLLGLLISAAFLFYAFRGQDYGAIVDSLRGVNYWLLLPA
                     LVLYILGVVARAFRWSVLLRPVKRIETKELLPITAIGFMANNVLPLRTGEVVRSFVLS
                     RNHGVRKTAALATIAVERICDGLTMVGFMLVAALFIDFTSELRHVMTIAVILFAIAIG
                     ILGVMTFAEGSRVRLLKFGLRFLPNSVGGRVEHIAESFFSGLAALRSPQAMGLVALSS
                     LVAWSFEASMYWVIARAFGGSVEAVMGVAETLLTTGVANLATLVPSSPGYVGPFEAGV
                     KTVVEGALKAPAAQALSYAILVHAALWFPVTLWGAVEWSRMHLSLKQVAAEEVAGPAM
                     AASGAHSETTPVREPATRTNAA"
     gene            1594..2460
                     /locus_tag="VFI12_05430"
     CDS             1594..2460
                     /locus_tag="VFI12_05430"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HET7055882.1"
                     /translation="MDTLEAVILGLVQGLTEFLPISSSGHLAIVPWLFKWDTPGLAFD
                     AALHFGTLLAVVIYFRAELIRMIRAVPLALRNLVPLLRGEAVDDPLAPDARLGLLIVI
                     GSIPGGILGLAANDAIDRFFHSESHQDRAMIVIAILLAAFGLLLWVAERVSRRDRPLS
                     DLRLADTVIIGIAQAIALFPGTSRSGVTMTAGMFRDLRRADAARFSFLLGLPLILAAS
                     LSGIKDLADSGASGVGATQIITGIVVSAVSGLAAIWGLLRFLQHAPTTVFTIYRVLAA
                     IGIMLLIATGVR"
     gene            complement(2759..4927)
                     /locus_tag="VFI12_05435"
     CDS             complement(2759..4927)
                     /locus_tag="VFI12_05435"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HET7055883.1"
                     /translation="MKIRPLLILLAIVLGMLIIAPPDTSAHANLESSAPAADSLLATP
                     PQEIELTFSEPVDMSGVTVTAVDESGQPVKLDQPRLDPTNDRRVLVSSSDFSIGAYTV
                     SWANRSLTDGHTLSGSFAFRVGGTDRAPAASTVEGQRPPAWAVVLRWLAFLGTAPAVG
                     ILLLFADPRRRRIAQGGLAVAVVVTLLDPVLLSAFPPQGSVGGSIIDAVRAEPTGWWV
                     RLVGLVLAFVLSLVPWRSESWRLAVGAAGLIGIGGLALTSHASGRETYAWAATGVAFL
                     HDAAVALWFGALALLVLAPEADRVKELGAFARRALPLAAVAVVAGLVNAGFIFPNIDT
                     ITSTDYGRVLIGKAVIVALVLGLATYHHLSLRGSLRALPSLMRNSVRLELGFIVLAVA
                     FASTLSLLAPPQVTHGELAKIDLAMPTSPEMTSDQIFVRLTIDPAKTGENTLTSYATQ
                     GPPMSVQTDANGAPKVVNESPLTDVQLMRIELSSIDLPIAPRAVDMTPLGDGRFQSSG
                     VNFSADGWWRAVVTVRRAGVAQDVTSEFILRVPHPNVIGFSQAATRKTDPTAEALYNK
                     AVAQFAQQQWVVLSQDLSGGNGGVEVSSQIWSNGGLKIATPNITMIRLNGKRYTTDKD
                     GNWRPPTDDQVPGGPSDWVTEFDGATDFTLGNQEEIDGQMTQAVHFYVPGTVLAPAFY
                     TWWVNVATGQIMQEAMISRSHYMIERLNWSAPPPAVTAPV"
BASE COUNT          827 a         1727 c         1633 g          913 t
ORIGIN      
        1 atctggggcc gcacggtctg gtgcggcgtg ccataaaaac agggagacgc gctttgccga
       61 agaaaccagc ctatcgtaag ccagttcgca acaccgcccc ggcgccgggc acggtgaccg
      121 aagaacgacc tcgccgccag gatcccgatg ccatcaccgt cgacggcaag gtggtcgatg
      181 cgttgccgaa cgccatgttc cgcgttgagc tcgagaacgg ccataccgtc ctcagccacc
      241 tcgccgggaa gatgcgcacc aactacatcc gcgtgctgcc gggcgatcgc gtcacggtcg
      301 agctgtcacc atacgacctc acccgcggcc ggatcaccta ccgccaccgc tagtcggcgc
      361 gtccgcaccc gtcgccgtct gtgacctccg ggctctcggc tcccagttct atagccacct
      421 atactcatcg ggccgcgcac ggaccgcggc cgagtgccac gtgcggtctg ggcgcgaatg
      481 ctccgcggtt atgaccggaa agcagttggg cctgtgcgct cctggcgatt tctcctcggt
      541 ctcctgatca gcgccgcctt cctgttctac gccttccgcg ggcaggacta cggcgcgatc
      601 gtcgactcct tgcgtggagt caactactgg ctgctgttgc cggccctggt cctctacatt
      661 ctcggcgttg tggcccgcgc cttccgctgg tctgtcctgc tccggccggt caagcgcatc
      721 gagaccaagg agctcctccc gatcactgcg atcgggttca tggccaacaa tgtgttgccg
      781 ttgcggaccg gcgaggtggt ccgttccttt gtcctctcgc gaaaccacgg cgtccgcaag
      841 accgccgccc tggccacgat cgccgtcgag cggatttgcg acggcctgac gatggtcggc
      901 ttcatgctgg tcgcggcgct cttcatcgat ttcacgtctg agctgcgcca cgtcatgacg
      961 attgccgtga tcctcttcgc aatcgcgatt ggcatcctgg gcgtgatgac cttcgccgaa
     1021 gggtcacgcg tccgactcct gaagttcgga ctgcgcttcc tgccgaattc cgtgggtggg
     1081 cgggtcgagc acatcgccga gtccttcttc tcgggcctgg ccgcgctgcg cagcccgcag
     1141 gcgatgggtc tggtcgccct ctcgtcgctc gtggcctggt cattcgaagc gtcgatgtac
     1201 tgggtgatcg cacgggcctt cgggggatcg gtcgaggcgg ttatgggcgt tgccgaaacc
     1261 ctcctgacga ccggcgtcgc caacctggcc acgctcgtac cctcgtcgcc cggctacgtc
     1321 gggccgttcg aggcgggcgt caagacggtc gtcgagggcg cgctcaaggc tcccgccgcg
     1381 caggcgctct cctacgcgat cctggtccac gccgcactct ggttcccggt cacgctctgg
     1441 ggtgcggtcg aatggtcgcg catgcatctc tcgctgaaac aggtcgccgc ggaggaagtg
     1501 gcgggaccgg ccatggcggc gtcgggcgct cattccgaaa ccacgcctgt ccgggagccg
     1561 gcaacgcgta cgaacgcggc ataattcgcg gtcgtggata cactggaggc cgtgatcctg
     1621 ggcctggtgc agggtctgac tgaatttctg cccatctcat catccggtca cctggcgatc
     1681 gtcccctggc tcttcaagtg ggacacgccc gggctcgcct tcgacgccgc cctgcacttc
     1741 ggcacgctat tggccgtcgt gatctatttc cgcgcggagt tgatccggat gatccgggcg
     1801 gtcccgctcg cattgcgcaa cctcgtcccg ctcctgcgcg gtgaagcagt cgacgacccg
     1861 ctcgcgcccg atgcccgcct cgggttgttg atcgtgattg gctcgatccc gggcggaatc
     1921 ctcggtttgg ccgcgaacga cgcgatcgac cgcttctttc actcagaatc gcaccaggac
     1981 cgcgcgatga tcgtgatcgc aatcctgctg gcggcattcg gtctgttgct ctgggtggcg
     2041 gagcgcgtct ctcgccggga ccggccactt tccgacttgc gcctcgccga cacggtcatc
     2101 atcggcatcg cgcaggcgat cgctctcttc ccgggcacgt cccgttccgg agtgacgatg
     2161 accgccggca tgtttcgcga cctgcgccgg gccgatgccg cccgcttctc attcctgctc
     2221 gggctgccgc tgatcctcgc cgccagcctg agcggcatca aggatctggc cgatagcggc
     2281 gcgtccggcg tcggcgccac gcagatcatc accgggatcg tcgtctccgc tgtctctggc
     2341 ctggcggcga tctggggtct gctccgcttc ctgcagcacg cgccgacgac cgtattcacg
     2401 atctaccgcg tcctcgccgc gatcggcatc atgcttttga tcgccaccgg cgtccgctaa
     2461 ccaccacagg ttgaattcgg ccccgtggtc gattagcgcg tctcgaccag tccgtttgca
     2521 ggcatcgcaa ccgagccgta gcgcggtccc ctcgtgggcc gcccgaaccc ggaaactgcc
     2581 cgatcgtgac cgggaaccaa ccgccgtggc cgggatcggc tccatccagc gggtatgggg
     2641 atgcgtgatg cggcccacgg ggggaccgcg ctacgcccca gatgaatcac tggctcttcc
     2701 caccgggcag gcggatgcac agggccgacc gccgggaccg cgctacggct ttctggaact
     2761 agaccggcgc cgtaacggcg ggcggtgggg ctgaccagtt taagcgctcg atcatgtagt
     2821 ggctgcggga gatcatcgct tcttgcatga tctgaccggt cgcgacattg acccaccacg
     2881 tgtagaaggc cggcgcaagg accgtgccgg gcacgtagaa gtgcacggcc tgcgtcatct
     2941 ggccgtcgat ctcttcctgg ttgccgaggg tgaaatcggt cgcgccgtcg aactccgtta
     3001 cccagtcgga tgggccgccc ggcacctggt cgtcggttgg tggccgccag ttgccgtcct
     3061 tgtcggttgt gtagcgcttg ccgttgagcc ggatcatggt gatgttcggc gtcgcaatct
     3121 tcagcccccc attcgaccag atctgtgacg aaacttcgac gcccccgttg ccgccagaca
     3181 agtcctggct gagcacgacc cactgctgct gcgcgaactg cgcgacggct ttgttgtaaa
     3241 gcgcctcggc agtagggtcg gtcttcctgg ttgcggcctg gctaaatccg atcacgttcg
     3301 gatgaggcac gcggaggatg aactccgacg tcacgtcctg cgcaactccg gcccgccgaa
     3361 cggtcaccac cgcgcgccac cagccgtccg cgctgaagtt gacgccggac gactggaacc
     3421 ggccatcgcc gaggggcgtc atgtcgaccg ctctcggcgc gatcggcagg tcgatcgacg
     3481 acagctcgat tcgcatcaat tgcacgtccg tcagcggcga ctcgttgacc actttcggcg
     3541 cgccatttgc gtcggtctga accgacatcg gcggcccctg cgtggcgtag gaggtaagcg
     3601 tgttctcgcc ggtcttggca gggtcgatcg tgaggcgcac gaagatttgg tcgctcgtca
     3661 tttccgggct ggtcggcatc gccaggtcga ttttcgccaa ttcgccgtgc gtgacttgcg
     3721 gcggagcgag caaggacagg gtcgaggcga aggcgacggc caacacgatg aatccgagct
     3781 cgagccggac cgagttccgc atcagggacg gcagtgctcg cagcgacccg cgcagggaca
     3841 ggtgatgata ggtggccagg ccaagcacga gggcgacgat gaccgccttg ccgatcagca
     3901 cccggccgta gtcggttgac gtaatcgtat cgatattcgg gaagatgaac ccggcgttga
     3961 cgaggccggc cacgactgcc accgccgcca acgggagcgc ccgccgcgcg aacgccccca
     4021 gctcctttac ccggtccgct tccggcgcaa ggacgagcaa tgccagggcg ccgaaccaga
     4081 gtgcaaccgc cgcgtcatgc aggaaagcga ccccggtcgc tgcccacgcg taggtttccc
     4141 gcccggaagc atgcgatgtc agggcaaggc caccgatccc aatcaacccg gccgcaccga
     4201 ctgcgagtcg ccagctctcg cttcgccacg ggacgaggct caggacgaac gccagtacaa
     4261 gaccaaccag gcgcacccac cagccagtcg gctcagcgcg gacggcgtcg atgatcgatc
     4321 caccgacgct cccctgaggc gggaatgccg agagcaagac cggatcgagc agcgtgacga
     4381 caaccgcgac cgccagtccg ccctgcgcga tccgtcgccg acgaggatcg gcaaagagca
     4441 acagtatccc gaccgccgga gccgtgccca ggaacgcgag ccagcgcagc acgacggccc
     4501 atgccggggg acgctgtccc tcgaccgtcg acgccgccgg cgcgcggtct gtcccgccga
     4561 cgcggaacgc gaacgatccg gagagggtgt ggccgtcggt cagtgagcgg tttgcccagc
     4621 tcacggtgta cgcgccgatc gaaaagtcgc tcgacgagac cagcacgcgg cgatcgtttg
     4681 ttggatcgag tcggggctgg tcgagcttga ccggctggcc tgattcgtcc accgctgtga
     4741 ccgtcacgcc ggacatgtcg accggctccg agaaggtcaa ctcgatctcc tggggcgggg
     4801 tcgccaaaag cgagtcggcc gccggagccg acgattcgag gttggcatgg gcagacgtgt
     4861 caggcggtgc aattatcagc atacccagca cgatggccag caaaatcagg agtggacgaa
     4921 tcttcacggg acgtacctcg cgccggacgg cgtgcgggga gtggtcgatc caaacgatcg
     4981 accgcgttct cttggcaata cgaatgtcgg aatcaggcag atgatcgcca tgtggcgata
     5041 ttacccgcac ggccggccgc aaaccgccgc tgaatcggcg ccaggcaacc ggaattgtgc
//
LOCUS       DASSGX010000309         4222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1362886, whole genome shotgun sequence.
ACCESSION   DASSGX010000309 DASSGX010000000
VERSION     DASSGX010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1362886"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..332)
                     /locus_tag="VFI12_05440"
     CDS             complement(162..332)
                     /locus_tag="VFI12_05440"
                     /inference="COORDINATES: protein motif:HMM:NF014898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HET7055884.1"
                     /translation="MPHSADPPISDLMTEFLEYQQSRNLSPYTLRNYRMTFASFRRFL
                     AETEVPDRASAA"
     gene            484..1197
                     /locus_tag="VFI12_05445"
     CDS             484..1197
                     /locus_tag="VFI12_05445"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET7055885.1"
                     /translation="MPANTKRKWPEIRHILIDADDTLWENNIYFEQATEEFVDFLDHS
                     TLSRDEVKTVLDDFERINARQFGYGSAVYTRSLQDCFRQLSERDIDETAIAVVMRIGR
                     RILEQELELTPHVETTLAHLGRDYVLTLCTKGDRDEQQIKIDRSGLTQYFHQIEIMAE
                     KDDSAYAGILDRLGAVPDDACMVGNSPKSDINPPLQLGMSAVFIPHDHTWQLEHQEVD
                     QSHPRLLVLRRFVELIEHF"
     gene            1294..1992
                     /locus_tag="VFI12_05450"
     CDS             1294..1992
                     /locus_tag="VFI12_05450"
                     /inference="COORDINATES: protein motif:HMM:NF013214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HET7055886.1"
                     /translation="MEQYRTAYPAQYVAQVGAEERSQFLRKVYQHVAGALAMFIVVEA
                     ILQSLPFTLNIAEQMSGVWLLVILGFWVVSWISARWTTPGLPLSQQYLGLGLFVLAEA
                     IIFMPLIAYVRLYQDSNVLPTAGVLTAALAVGLSLIAFDSRTDFSMLRGVLFIGAPVA
                     LGLILASILFGVGLGAWFSLAMIGLASASILYKTDEITKTSRTDSYVSAALILFSSFM
                     LLLWYVIRLLSSRR"
     gene            complement(2059..2322)
                     /locus_tag="VFI12_05455"
     CDS             complement(2059..2322)
                     /locus_tag="VFI12_05455"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET7055887.1"
                     /translation="MNIILWIIFGALAGWLASLINGTNREQGWIGNIVLGIVGAFVGG
                     IIWNLITGDSYTSGFNVGSLIIAIIGALIVSWLFSMFTGRRAV"
     gene            2491..2961
                     /locus_tag="VFI12_05460"
     CDS             2491..2961
                     /locus_tag="VFI12_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055888.1"
                     /translation="MHSVLLTACLDRTWPNLAHTSDCRVGVHALGGQPRRYDCARAPQ
                     TAAAMDGDRIPFGVTLGYGSDCRVEDGRRWRGKVSNRQMDLLDAEGAQCSRIVTPAGK
                     VDDAPDSHAGQPIEPGLPGGVGADYQEAGYDPVALVSPHDCLQYRVYVHLTLPL"
     gene            3263..4093
                     /locus_tag="VFI12_05465"
     CDS             3263..4093
                     /locus_tag="VFI12_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HET7055889.1"
                     /translation="MLDSFGVRWQYEPTTFPLVTSETGQLVQCFTPDFFLPEHGVYIE
                     MTTMRQSLVTRKNRKFRLMRELYPDVNVRLLYRKDVELIMERYDVRNDSVVGWPGPVA
                     ATAEQMSRKAEEAATFLAGGCQEPIALIALNSSAERFRDHVAAGLKAHGTSVASSRVT
                     VTRSRKPGAASSAAVRFSGTVDLRACRRVLVADVIGTGLTVRAALDWLAEAGIPASEV
                     ITLFDRRSARIIDVPLAVPGLPAASLWLVGAGLGGGPWARMPDVHTITAADLTPMTTN
                     "
BASE COUNT          765 a         1317 c         1326 g          814 t
ORIGIN      
        1 gctgtgagtt gccaccagtt gccgaccggt tgccgatagg agcgcatggt aagggtgcgc
       61 tccccctaag aagctggcgg ctctacggcg aacgcggcct gcccggcagc gagcagttcg
      121 tgcagtccca gcgacgagcc gctggcatag gacacggtca cctacgctgc cgacgcccgg
      181 tccggaacct cggtttcagc gaggaaacgg cgaaagctgg cgaaggtcat gcggtagttg
      241 cgcaaggtgt agggagagag gttgcggctc tgctgatact cgaggaattc cgtcatgagg
      301 tccgagatgg gcgggtcagc tgagtggggc atcggacttc tccggtcctt gatgtccttg
      361 aacgagcatc tggagggtgc cccgggctga ctcgcttccg ccggagtccg ggggaccgcc
      421 gactatactc ctggtctgga agtagacgac acttccgctc ggctgccata agaaacatcg
      481 tctatgcccg caaacaccaa gcgcaaatgg ccggaaatcc gccacatcct gatcgacgcc
      541 gacgacacgc tctgggaaaa caacatctac ttcgagcagg cgaccgagga atttgtcgac
      601 ttcctcgacc attcgaccct gagccgggat gaggtcaaaa cggtcctcga cgacttcgag
      661 cggatcaatg cccggcagtt cgggtacggc tcggcggtct acacccgctc gctgcaggat
      721 tgcttccggc aactttcgga gcgggatatc gacgagaccg ccatcgcggt tgtgatgcgg
      781 atcgggcggc ggatcctcga gcaggagctg gagctgaccc cgcacgtcga aacgacgctc
      841 gcgcaccttg gtcgcgatta tgtcctcacg ctctgcacca aaggagaccg ggacgagcag
      901 cagatcaaga tcgatcgctc cggcctgacg caatacttcc atcaaatcga aatcatggcg
      961 gagaaagacg actctgccta cgccggcatc ctcgatcggc tcggcgcggt tccggatgac
     1021 gcgtgcatgg tcggtaactc cccgaagtcc gatatcaacc caccactgca gctcggaatg
     1081 tcggccgttt tcattcctca cgaccacacc tggcagctcg aacatcagga ggtcgaccag
     1141 tcgcatccgc gcttgctggt cttgcgtcga ttcgtggaac tgatcgagca cttttgaccc
     1201 taatttgcca tcgacgggcg ggtcagaagg ggataggctt tacatatgcg tccgaacgca
     1261 gcgccgcgtt cctgcaattg aaggagtaac gagatggaac agtatcgaac ggcctatccg
     1321 gcgcaatacg ttgcgcaggt cggtgccgag gagcgatcgc agttcctccg caaggtgtac
     1381 cagcacgttg cgggtgcctt ggcgatgttt attgtcgttg aggccatcct gcaatcgctt
     1441 ccattcacct tgaacattgc cgagcagatg agcggtgtct ggttgctcgt catcctcggc
     1501 ttctgggtgg tgagctggat ttcagcccgc tggaccaccc ccggcctgcc gctctcccag
     1561 caatacctcg gtcttggcct gttcgtcctg gccgaagcga tcatcttcat gccgctgatc
     1621 gcctacgtgc ggctgtacca ggacagtaat gtcctgccga cggcgggcgt gctgacggcg
     1681 gcgctggcag tcggcctatc tctgatcgcg ttcgattccc ggaccgactt cagcatgttg
     1741 cgcggcgtgc tgttcatcgg cgcgcccgtg gcgctcggac tcatcctggc gtcgatcctg
     1801 ttcggtgtcg ggctgggcgc gtggttctcg ctggcgatga ttgggctggc ctcggcgagc
     1861 atcctctaca agaccgacga gatcacgaag acctcgcgca ccgacagcta cgtgtcggcc
     1921 gcgctcatcc tgttctcgtc gttcatgctc ctgctctggt acgtgatccg gctgctgtcg
     1981 tcgcgccgat agtcgtctca aaacggaacg aaaacggccc cgggatttcc cggggccgtt
     2041 ttcgtgttgt gcgctgggct agaccgctcg gcggccggta aacatgctga acagccagct
     2101 gacgatcagg gcgccgatga tggcgatgat caggctcccg acgttgaagc cgctggtgta
     2161 gctatcgccg gtgatcaggt tccagatgat gccgccgacg aacgcgccga cgataccgag
     2221 gacgatgttg ccgatccagc cttgctcccg gttggtgcca ttgatcaggc tcgccagcca
     2281 cccggccagc gcgccgaaga ttatccacag gatgatgttc atgctatctc tccttgggtt
     2341 accgatctgc cacgtcgcag cggtcgtctg cgggcaacga tagagagatt cgcggacgtt
     2401 ctcagtgcgc agcctacaca ttcgggttgg cgctgaagtc gggtcaaggc tgcttcaccc
     2461 tctgtccgat gcatgccgga cgacgaattt gtgcacagcg tgctcctgac ggcgtgtctc
     2521 gatcgaacct ggccgaatct cgcgcacacg tcggattgcc gcgtcggcgt ccatgccctg
     2581 ggaggccaac cacgccgcta cgactgtgcc cgtgcgcccc aaaccgccgc ggcaatggac
     2641 ggcgaccgca tcccctttgg agtgactctc ggttatggca gcgactgccg cgtcgaggac
     2701 ggcaggcgat ggcgcggtaa agtctcgaac cggcagatgg acctcctcga tgccgagggc
     2761 gcccaatgta gccggatcgt gacgccggct ggcaaggttg acgacgcgcc ggactcccat
     2821 gcgggccagc cgatcgagcc cggcctcccc ggcggggtag gggcagacta tcaggaggcc
     2881 gggtacgatc cagtcgccct ggtcagccct cacgactgtc tgcagtacag agtgtacgta
     2941 cacctaactc taccattgtg acctcctgtg agcgtccgta cagttgtcgg ctgcgagaac
     3001 gtatgcaaaa ctgcgtatag ccccgaaata ctcgcacagt gcttcaacag attgctgccg
     3061 gaacgggcag cggtgcgagg gggaattggt ggcagaagcg tggccgagcg acgacgcgcg
     3121 aacactattg tcgcggccta ccactgaacg tacacgctac aacatccccc attcaacgcg
     3181 acgacaatca gcgagttccg gcgaccgaag ggtcgagtgt ccgacccgct ttgcgcatcc
     3241 ggccgaggcc gttgtcgccg gcttgttgga ttccttcggc gttcgctggc agtatgaacc
     3301 caccaccttt ccgctcgtca cgtccgagac tggccagctc gtccagtgct ttacgcccga
     3361 cttctttttg cccgaacacg gcgtctacat cgagatgacc acgatgcggc agtcgctcgt
     3421 cacccgcaag aaccgcaagt tccggttgat gcgagagctg tatcccgatg tgaatgtgcg
     3481 cctgctctat cgcaaggacg tcgagctcat catggagcgg tacgacgtcc ggaacgactc
     3541 ggttgtgggg tggcccggcc cggtcgcggc gaccgccgag cagatgtccc gcaaggctga
     3601 ggaagcggca acattcctgg ccggcggttg ccaggaaccc atcgcgttga tcgcgctgaa
     3661 cagcagcgcg gagcgattcc gggatcacgt cgcggccggg ctcaaggcac atgggacgtc
     3721 cgtcgctagc agccgcgtga ccgtgacacg ctcccggaaa ccgggcgccg cctcgtcggc
     3781 cgccgtgcgc ttcagtggga ccgtcgatct gcgggcatgc cggcgcgtcc ttgtggccga
     3841 cgtaattggc accgggctga ccgtccgcgc cgccctcgat tggctggcgg aagccgggat
     3901 tcccgcctcc gaagtgatca ccctgttcga tcggcggagc gcgcggatca tcgatgtgcc
     3961 cctggcggta cccggattgc ccgcggcgtc actttggctg gtcggcgctg gattgggtgg
     4021 cggaccctgg gcgcggatgc ccgatgtgca cacgatcacg gcggcggatc tcacaccgat
     4081 gacgaccaac tagttcgcgg cacagattga cgagttcgga ccccgatgcc aacgattgcc
     4141 ggctgcgtca gcatcgcgat cgtcgcgggg acacttgcac tgatcctgat acggccacgc
     4201 gggttgaatg aagcatgggc tg
//
LOCUS       DASSGX010000310         2153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1472861, whole genome shotgun sequence.
ACCESSION   DASSGX010000310 DASSGX010000000
VERSION     DASSGX010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1472861"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1636)
                     /locus_tag="VFI12_05470"
     CDS             complement(<1..1636)
                     /locus_tag="VFI12_05470"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HET7055890.1"
                     /translation="MRVYVLDFQEIDQMRVAAVGGKGVHLGELSRIEGIRVPPGFCVT
                     TDAFQRIMAEAPSIDDRLDRLSRLKPDDREAIRALSTEIRRTLEGVAIPGDLAAAITR
                     ALARLGEQGAYAVRSSATAEDLPTASFAGQQDTYLNVVGPAAILQHVSRCWASLFTER
                     AVTYRLRNGFGHRKVHMAVVVQRMVFPRAAGILFTADPVTGNRKVASIEAGFGLGEAL
                     VSGRVNADIYKVRDGEVVARAIATKRPALTDAQAVRLAQLGRRIEAHFGRPQDIEWCL
                     VSDDFQIVQSRPITTLFPIPQTGDRESHVYVSVGHQQMMTDPMKPLGLSFWQMTTPRP
                     MAEAGGRLFVDVTQILASPASRPGLLAMLGRSDPLIGDALQTILGRGDFIPSLPDAGP
                     AWTPPGGGAASLETDPAIVEELIGSSQAAIAALKRDIRTRSGPALLDLIRASIQELKP
                     ADPRSMQVVMAGMEATWWLNDRMQAWLGEKNAADTLTQSVPNNVTSEMGLALLDVADA
                     IRPHPDVVAFLQNVEDEGFLDELPKIAGGREARDAIQ"
     gene            1864..>2153
                     /locus_tag="VFI12_05475"
     CDS             1864..>2153
                     /locus_tag="VFI12_05475"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HET7055891.1"
                     /translation="MKILIFGATGSAGGSVLRACLASPAVEEVRALARRPLAMADPKL
                     RAIVHDDYLDYSRVESAFAGVDACLFCLGISITQVSGEAEYRRITHDFALAAA"
BASE COUNT          321 a          753 c          734 g          345 t
ORIGIN      
        1 cctggatggc gtctcgcgct tcccggccgc ccgcgatctt ggggagctcg tccaggaagc
       61 cttcgtcctc gacgttctgc agaaaggcca ccacgtccgg atgcgggcgg atcgcgtccg
      121 cgacgtccag gagcgccagc cccatctcgg acgtgacgtt gttggggacg gactgcgtga
      181 gcgtgtcggc cgcgttcttc tcacccagcc acgcctgcat ccggtcgttc agccaccagg
      241 tggcctccat ccccgccatg accacctgca tgctccgcgg atcggccggc ttcagctcct
      301 ggatgctcgc ccggatcaaa tcgagcagcg ccgggccgga ccgcgtgcgg atgtcgcgct
      361 tcaaggcggc gatggcggcc tggctgctcc cgatcagctc ctcgacgatg gccggatcgg
      421 tctcgagcga ggcagccccg ccgccgggcg gcgtccaggc ggggcccgcg tccgggagcg
      481 acggaatgaa gtcgccgcgg ccgagaatgg tttgcagcgc gtccccgatc aatgggtccg
      541 accttccgag catcgccagg aggcccgggc ggctcgccgg cgaggccagg atctgggtga
      601 cgtcgacgaa caacctcccg ccggcctcgg ccatgggccg cggggttgtc atctgccaga
      661 aggagagccc cagcggcttc atgggatcgg tcatcatctg ctgatgaccg acggagacgt
      721 agacgtggct ctcccggtcg ccggtctggg ggatggggaa cagcgtggtg atcggccggc
      781 tctggacgat ctggaaatcg tcgctaacca ggcaccattc gatgtcctgg ggacggccga
      841 aatgtgcttc gatccgccgg cccagttgcg cgagccgcac ggcctgcgcg tccgtcagcg
      901 ccggccgctt ggtggcgatc gccctggcga cgacctcgcc gtcccgcacc ttgtagatgt
      961 ccgcgttcac ccggccggag accagggcct cgccgaggcc gaagccggcc tcaatggagg
     1021 cgaccttccg gttgcccgtg acggggtcgg cggtgaacag gatgccggcc gcccgcggga
     1081 agaccatccg ctgcacgacc acggccatat ggaccttccg gtggccgaag ccgttccgca
     1141 ggcggtaggt cacggcccgc tcggtgaaga gagaggccca gcaccggctg acgtgctgga
     1201 ggatcgccgc cggccccacg acgttcaggt acgtgtcctg ctggcccgcg aaggaggctg
     1261 tcggcaggtc ctccgccgtc gcgctggacc ggacggcgta ggcgccttgc tcgccgagcc
     1321 gggcgagcgc gcgggtgatc gccgccgcca gatcgccggg gatggcgacc ccttcgaggg
     1381 tccggcggat ttccgtgctg agcgcgcgga tcgcctcccg gtcgtccggc ttcaggcgcg
     1441 acagccgatc gagccgatcg tcgatcgacg gcgcttccgc cataatccgc tggaaggcgt
     1501 ctgtcgtcac gcaaaagccg ggcggcacgc ggatgccttc gatccgcgaa agctccccca
     1561 ggtgcacgcc cttgccgccg acggccgcga cccgcatctg atcgatctcc tggaaatcca
     1621 atacatacac acgcatgccg cgcctcccat tcccggcggc cagaggatct ggccccggcc
     1681 cgcgaaccta cggcacggcc cgggtcttgc cgcaagcccc ccggtgcgct atattctgaa
     1741 agtgggaaga agggggtacg cgtcttcctg ccccccgaaa ccatccaaaa agacaactgg
     1801 catcatcatc gcgctggccg agccgctcag ttgaagctag aggcagtagc gcgtgaccgt
     1861 ctcatgaaaa tcctgatctt cggcgcgacg ggctcggcgg gcgggagtgt cctgcgcgcc
     1921 tgtctcgcct ctccggccgt cgaggaggtg cgcgccctcg cgcgccgtcc tctcgcgatg
     1981 gccgatccga agctccgggc gatcgtgcac gacgactatc tggattattc gcgggtggag
     2041 agcgccttcg cgggggtgga cgcctgcctc ttctgcctcg gcatttcgat cacccaggtc
     2101 tcgggcgagg cggagtaccg gcggatcacg cacgatttcg cgctggccgc cgc
//
LOCUS       DASSGX010000311         5135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_600846, whole genome shotgun sequence.
ACCESSION   DASSGX010000311 DASSGX010000000
VERSION     DASSGX010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5135
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600846"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1351
                     /locus_tag="VFI12_05480"
     CDS             <1..1351
                     /locus_tag="VFI12_05480"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET7055892.1"
                     /translation="HRSGPTLLLDMLIAGGGFLLYELGRIATSNGESRAFENAERMIA
                     VQKILHLPDEAAFQQFIISHELLLRVVNKVYSFFFLSTVIGILVWLYLNRPAVYRRVR
                     NALGLSTLIAIAVFAVWPMAPPRLTPASNMIDSHARVGARHGFVNQFAALPSLHIGWL
                     SLVGWGMWAGLGRVRGMLIALIPPAIMMVAVIGTGNHFWLDGVLGAALCLGAVAATGP
                     RLVPAPAAVQHAAERLSFPALLAQRERVRNVITVLCGLLIFTFLGRVLDPVFTPYWGY
                     LAGQVALLGLAILLIERRCRTHPLLSHSTYTIISFAITVDVLGTAGHMYERVDFYDKI
                     VHVMGTAAVTAVLHDIFAYQARGRSRMQPYALAAIAAALGISVGVIWEVYEVFGDVLF
                     ATARSGGFWDTTYDLIFDTIGAVSAAIILPWRLGEPDTIGPALEPPPAATELAGSGQ"
     gene            complement(1331..2830)
                     /locus_tag="VFI12_05485"
     CDS             complement(1331..2830)
                     /locus_tag="VFI12_05485"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HET7055893.1"
                     /translation="MRERRTLCIGIILLALPLAAFGLLHWIATGADAQIVVPTEHFLA
                     VSAACIVALLLAVVFSYAAVRTREPRTFFLAATYLMIAAPFSVHGLMTPGQTFSMHGF
                     HNSMVVSAQTSLVLGALALLLASRPLPRLLDDAIRDQFGPLMAGLVLLSFGYVLLCLS
                     VPTILDWAPTGGEPAGVTTVFGLDRRAVGLSIRYALMGLGIVLSLIAAVRFYRSFATT
                     RSFATAAVTITAVLLAECLFIQSFGIVWNLSWWLYHGLLLIAVVLPMTAFALLYRRGS
                     SLIAIVDSLLLTETLAKVEYSFPDAIEGFITTVEHRDPYLKGHMRRVCELAVAIADQM
                     RAPDTVIRAASYAALLHDIGKLGLPHAVLNKPGRLTDDEFAVLKQHPTRGFALVANIE
                     SLRSAAPAIRWHHERLDGTGYPDALAGDAVPLEARIIAVADVWDALTSDRVYRKAMSH
                     AEAQAILISERGTKLDGDCVDALLAVVGRAVIAAEDAAAPFTRSLAAAG"
     gene            3320..4477
                     /locus_tag="VFI12_05490"
     CDS             3320..4477
                     /locus_tag="VFI12_05490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HET7055894.1"
                     /translation="MSSATASPSETTLPKPEGLEGVVAASTALSHVFGEEGKLVYRGY
                     DIHELAGKASFEEVAHLLWIGHLPNKQELAAFQAKLEANRELPDAVIDVLRLLPAETP
                     PMDALRTGLSALGAIDSERFDETPDLDEAIALQARFPGILAAFFRLRSGEQPVKSKSG
                     HNTAQSYLYQLFGKEPEERHWKPLDTYLVLLADHGMNASTFTARVIASTQSDLVSALT
                     GAVGALKGPLHGGAPSKVLDMLHAIGTADNVEGWLTNALDNGERLMGFGHRVYKAEDP
                     RAEILREMAKTASDPDFFKLSKLTEDRALQMLNERKPGRRLYTNVEFYSAAVLAAVGL
                     PGDMFTPTFAVSRSVGWSAHVLEQVSNNRLIRPQSQFVGPHDKKFAPIEKR"
BASE COUNT          915 a         1734 c         1638 g          848 t
ORIGIN      
        1 tcaccgatcc gggccgacgc tcctgctcga catgctgatc gccggtggcg gatttctgct
       61 gtacgagctg ggaaggatcg cgaccagcaa cggcgaatcg cgtgccttcg agaatgccga
      121 gcggatgatc gccgtccaga agatcctcca cttgccagat gaggcggcgt tccagcagtt
      181 catcatctcg cacgagttgc tcctccgcgt ggtcaacaag gtctactcgt tcttcttcct
      241 ctcgacggtg atcgggatcc tggtctggct ctacctgaac cggccggctg tgtaccggcg
      301 agtccgcaat gcgctggggc tgagcacgct gatcgcgatc gcggtcttcg ccgtctggcc
      361 gatggcgccg ccacgcctga ccccggccag caacatgatc gacagccacg cccgggtcgg
      421 cgcgcggcac ggcttcgtca accagttcgc ggcattgccc agcctccaca tcggctggct
      481 ctcgctcgtc ggctggggca tgtgggcggg ccttggacgg gtgcggggaa tgctcatcgc
      541 gctcattccg ccggcgatca tgatggtcgc cgtgatcggg accggaaacc acttctggct
      601 ggacggcgtg ctcggtgcgg ccctctgcct cggtgccgtg gccgccaccg gtccccgcct
      661 cgtcccggcg ccggcggccg ttcagcacgc ggccgagcgg ctatcgttcc cagccctgct
      721 ggcccagcgc gagcgagtcc ggaacgtgat cacggtcctc tgcggcctgc tgatcttcac
      781 cttcctcggc cgcgtgctgg atccagtctt cacgccatac tggggctacc tggcggggca
      841 agtcgcactt ctcggcctgg caatcctgct catcgagcgc cggtgccgga cgcacccgct
      901 gctcagccac tcgacctaca cgatcatctc attcgcaata accgtcgatg tgctgggcac
      961 ggccgggcac atgtacgagc gcgtcgactt ttatgacaag attgtccacg tcatgggcac
     1021 cgccgcggtg acggccgtac tccacgacat cttcgcgtac caggctcggg gccggagccg
     1081 aatgcaaccg tacgccctcg cggccatcgc ggcggcgctc gggatctcgg tcggcgtgat
     1141 ctgggaggta tacgaagtct tcggcgacgt gctgttcgcg accgcccgga gtggcggctt
     1201 ctgggacacg acctacgacc tgatcttcga cacgatcggg gccgtcagcg cggcgatcat
     1261 cctgccctgg cgcctgggcg aaccggacac gattgggccc gccctggagc cgccgcctgc
     1321 cgcgaccgaa ctagccggca gcggccagtg agcgggtaaa cggggccgcc gcatcttccg
     1381 cagcgatgac tgctcttcca acgacggcca acagcgcgtc gacgcaatcg ccgtcgagct
     1441 tcgtgccccg ctcagagatc aggatggcct gggcttcggc atgggacatc gccttccggt
     1501 acacccggtc cgaggtgagc gcgtcccaga catcggctac ggcgatgatt cgcgcctcca
     1561 gcggcaccgc atcgccggcc agcgcgtcgg gatagcccgt gccgtcgagt cgctcatggt
     1621 gccagcgaat cgcaggagcg gcactgcgca ggctctcgat gttggcgacg agggcgaagc
     1681 cgcgcgtcgg gtgctgcttc aagaccgcaa actcgtcgtc ggtcaggcgg ccgggcttgt
     1741 tcaggacagc gtgcggaagg ccgagcttgc caatgtcgtg cagcagggcc gcgtagctgg
     1801 cggcgcggat cacggtatcc ggcgcgcgca tctggtcggc gatcgcgacg gccagctcgc
     1861 aaacccggcg catatggccc ttgagatacg gatcgcggtg ttcgactgtc gtgatgaaac
     1921 cctcgatcgc atccggaaag gagtattcga ccttggccag cgtctcggtc aagagcaagg
     1981 aatcgacaat cgcgatcagc gagctgcccc ggcgatagag caacgcgaag gcggtcatcg
     2041 gcaggacgac cgcgatcagc agcaagccat ggtacagcca ccaggagaga ttccagacaa
     2101 tcccgaacga ctggatgaaa aggcactccg cgagcagtac ggcggtgatc gtaaccgctg
     2161 ccgtcgcgaa ggatcgggtc gtcgcgaacg accggtagaa gcgaacagcc gcgatcaggc
     2221 tcaacacgat acccaggccc atgagcgcgt accggatgct caggccaacc gcgcggcggt
     2281 cgagcccgaa gactgtcgtc acgccggccg gctcaccccc agtcggcgcc cagtcgagga
     2341 tcgtggggac cgagaggcag agcaggacgt agccgaacga gagcaggacg agcccggcca
     2401 tcaggggacc gaactggtcg cgaatcgcat cgtcgagcag gcgtggcaac ggccgcgagg
     2461 ccagcaacag cgccagcgcg ccgagtacga gcgacgtctg ggcgctgacc accatgctgt
     2521 tatggaagcc gtgcatggag aaggtctgac cgggcgtcat caacccgtgg acgctgaagg
     2581 gcgcggcgat catcaggtag gtcgcggcca ggaagaaggt gcgcggttcc cgggtccgca
     2641 ccgccgcgta gctgaagacg acggccagca gcaaggccac gatgcaggcg gccgagacgg
     2701 cgagaaagtg ctcggtcggc acgacgatct gcgcatcggc accggttgcg atccagtgca
     2761 gcaggccgaa cgccgccagc ggcagcgcga gcaggatgat gccgatgcag agcgtgcggc
     2821 gctcacgcac tgcatcctga tcacttgata cggcgacgct ccggaccgga gtgcgtcgca
     2881 acatgcgcag cgacatccac tgtccttgct caaggcacgc ggcaatccgg ctcgatcgta
     2941 ggcaggaccc ggactaccgg catatccccc gaatgtcagg gatcagacga tgtcgaaagg
     3001 tctctcgtca ttggtctctc gtctttcgtc tttggttttc cgttttccgt atccatctac
     3061 caacgtccaa cgtccatcta ccctcagtcc cggctgctcg cctgatagac acatgacccc
     3121 gtgcattcct gtccggcttc aacagggcaa agtgctagga tgacgctgac tgccgcagcg
     3181 tgtgcggctc ccgctcgacg atcgagagac aaccaggatc gcggtcattt ccgaccaggg
     3241 cggaatcagg atctgagcac gcgttgatca ggagctggag aggcgacgat cgatcggcgc
     3301 gggaacagga gcaggatcga tgtcctcggc gaccgcatct cccagcgaga ccacacttcc
     3361 caagccggaa ggactcgagg gtgtcgtcgc tgcctcgacg gcccttagcc atgtcttcgg
     3421 cgaagagggc aagctggtct atcgcggcta cgacattcac gagctggccg gcaaggcgtc
     3481 attcgaggag gtcgcccacc ttctctggat cgggcatctc ccgaataaac aggaactggc
     3541 cgcattccag gccaagctgg aggccaaccg cgagctcccg gacgcggtga tcgatgtcct
     3601 ccgcctgctg ccggccgaga ccccgccgat ggatgcgctg cggaccggcc tttcggcgct
     3661 cggcgcgatc gactcggagc gattcgacga gacgccggac ctcgatgagg cgatcgcact
     3721 ccaggcccgc ttcccgggca tcctggcggc attcttccgc ctccgcagcg gcgagcagcc
     3781 ggtcaagtcc aaatcgggac acaacacggc ccagagctac ctctaccagc tcttcggcaa
     3841 ggagccggag gaacgccact ggaagccgct cgacacctac ctcgtcctgc tggccgatca
     3901 cggcatgaat gcctcgacct tcacggcccg ggtgatcgcc tcgacccagt cggacctggt
     3961 ctcggccctg accggcgcgg tcggcgcgct caagggtccg ctccacggcg gcgccccatc
     4021 caaggtcctc gacatgctcc acgcgatcgg cacggccgac aacgtcgagg gctggttgac
     4081 caacgcgctc gacaacggcg agcggctgat gggcttcggc caccgcgtct acaaggcgga
     4141 ggacccgcgc gccgaaatcc tgcgcgagat ggccaaaacc gccagcgatc cggacttctt
     4201 caagctctcg aagctgaccg aggaccgggc cctccagatg cttaacgagc gcaagccggg
     4261 ccggcgcctc tacaccaatg tcgagttcta ctcggcggcg gtgctggcgg cagtcggatt
     4321 gccgggcgat atgttcaccc cgacgtttgc cgtctcccgc tcggtcggct ggagcgcgca
     4381 cgtgctggag caggtcagca ataaccgcct gatccgcccc cagtcgcaat tcgtcggccc
     4441 gcacgacaag aagttcgccc cgattgagaa gcggtaaacc agcgatccaa tcacggggcc
     4501 atccgatatc ggatggcccc gttgccaggc ctgagatacc ggctcgtaac cgcgatcgag
     4561 ctctccggcg ccacccggag ataaatgccc gggctgaaca aggggaagcc cactgaaggg
     4621 gctgggacgg gcgctcgctc tgtctaaacg tagccccggg cggcctcgcc cggtgttcca
     4681 cggagactcg ttccattgcc accgagccct aatcaccgct ccgggctgaa ggccggggct
     4741 gagccaaaat aaggcggcta aagccgaccc gccttctctt ggctggacct ctcgttccgc
     4801 agcccggcgc tttgagcccc gggcgatgcc gaacagcccg atccaagtcg acgtacgcgc
     4861 ccacgaatag ttcccgctca gggtagagtt ctcgcagcaa actgaatctc agcgttggat
     4921 cgattcgcgt gcccttcgtt tcggcttcat cttcacggcg agttgagaca tgttccgcgg
     4981 cctgagcctc tcggcactgg cggccgtcgt ctgctgggca gcgatggcgc cgttcgccaa
     5041 gtacgccttc gactccttct cggccgatgc ctacctcggc ttgcgcccga tcatcgccac
     5101 cgccgccgcc ttcaccgtca tggcggcccg cggcg
//
LOCUS       DASSGX010000312         2480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_273926, whole genome shotgun sequence.
ACCESSION   DASSGX010000312 DASSGX010000000
VERSION     DASSGX010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273926"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /gene="rsmG"
                     /locus_tag="VFI12_05495"
     CDS             <1..686
                     /gene="rsmG"
                     /locus_tag="VFI12_05495"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HET7055895.1"
                     /translation="PTANCMKGGRRLRRIDGPEAFQRALAVSRETVDRLIMYENLLRQ
                     WQRAVNLVAPGTIPDIWERHFADSAQLARLVPEDAASLVDLGSGGGFPGLVLAIMLAE
                     RDRIRVSLIESDTRKAAFLREVARQTGVPVDILATRIENPETQRKVGRVDVVTVRALA
                     PLSRLLELAAPFFAPRTVGLFLKGRDIHGEIDEARVSWRVDVELVQSMTDSQGQIAVV
                     RNVAAKTEG"
     gene            690..1520
                     /locus_tag="VFI12_05500"
     CDS             690..1520
                     /locus_tag="VFI12_05500"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7055896.1"
                     /translation="MNGKTMGSQGLRVIAIANQKGGVGKTTTAINLGTALAASGERVL
                     VVDLDPQGNASTGLGIVLEDRRRTTFDVMTGRCSLADAAIGTQVPGLSIVPANSDLVG
                     IEKELMNEPTRPFRMRDAVGALVGHQRSRPEAAVTYVLIDCPPSLNLLTLNAMVAAHA
                     VLVPVQCEFFALEGISQLRENIEQIKASLNPGLEIQGVVLTMHDQRTALSREVAQNVR
                     AFFGAKVYDTMIPRNTRVAEAPSHGMPILLYDVNSAGSQAYMRLAAEIIERERRYKAA
                     "
     gene            1562..2434
                     /locus_tag="VFI12_05505"
     CDS             1562..2434
                     /locus_tag="VFI12_05505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HET7055897.1"
                     /translation="MAAPLQKGRLGRGLASLIGDPVLAEPKLPSEGEQRFVPIDQIRG
                     GKLNPRREFKEEDLAELAESIRQKGLVQPIVVRPDRLVGGYEVVAGERRWRAAQRVGL
                     HSIPCIVRELSDQEVLELGIIENVQRADLNPIEEAAGYRELIDRFGYTQDQLADVIGK
                     SRSHLANTLRLLKLPDTVRILVQEGKLTAGHARALVGREDAEALAERIIDRDMNVREV
                     EALVQSGGIGQPSRRRDKNADVLAFEKELSDVLGLKVEIKRGSGESGVLAIKYGNYDQ
                     LDYIRLRLIGSQVE"
BASE COUNT          462 a          742 c          836 g          440 t
ORIGIN      
        1 cgccaacggc aaattgcatg aagggcggtc gccgcctccg gcggatcgat ggaccggagg
       61 ccttccagcg cgcgttggcg gtttcacgtg aaaccgtcga tcgactgatc atgtacgaaa
      121 atctgctgcg gcagtggcag agagcagtca acctcgtcgc ccctggaacc atcccggaca
      181 tctgggaacg ccatttcgcc gacagtgctc agctcgcacg gctcgttccg gaggacgcgg
      241 cgagcctcgt cgacctgggc tctggtgggg gctttccagg tctggtgctc gccatcatgc
      301 tcgcggagcg ggaccggatc cgtgtcagcc tcatcgaaag cgacacgcgc aaggccgcat
      361 tcctgcgcga ggtcgcgcgt cagaccgggg tgcctgtgga catcctcgca acgaggatcg
      421 aaaacccgga gactcagcgt aaagtgggcc gagtcgatgt agtcacggtg cgtgctcttg
      481 ctccgttgag ccgcctgctc gagctggcgg ccccgttctt tgcgcctcga actgtcggtc
      541 tgttcctcaa ggggcgcgat atccacggtg aaatcgacga ggcaagggtc agctggcggg
      601 tcgatgtcga gctcgtgcag agcatgacgg acagccaagg ccaaatcgcc gtggtgcgca
      661 atgtggcagc caagacggag ggttgagccg tgaacggcaa gaccatggga tcccagggac
      721 tgcgggtgat cgcgatcgcc aaccagaagg gtggcgtcgg caagacgacc acggccatca
      781 atttgggtac ggcgctggcc gcgtccggcg aacgggtgct cgtcgtggat ctcgatccgc
      841 aaggcaatgc ctcgaccgga ctcggcatcg tcctagagga tcgccgcaga acaacctttg
      901 acgtcatgac gggtaggtgc agtctggccg acgcagccat cggcacccag gttcctggat
      961 tgtcgatcgt gccggccaat tccgacctgg tcggcatcga gaaggagttg atgaacgagc
     1021 caacccggcc attccggatg cgcgatgccg tcggagctct cgttggacat cagcggtcga
     1081 ggcccgaggc cgctgtcacc tacgtgctca tcgattgccc gccctcgctt aacctgctga
     1141 cgctcaatgc catggtcgcg gcgcatgcgg tgctggtgcc ggtccagtgc gagttcttcg
     1201 ccctggaggg catttcccag ctcagggaga acatcgagca gatcaaggcc tcgctcaatc
     1261 cgggtctcga gattcagggc gtcgtgctga cgatgcacga tcagcgcacc gctctgtcgc
     1321 gagaggttgc ccagaacgta cgcgcattct tcggtgccaa ggtttacgac acgatgatcc
     1381 cccggaatac gcgggttgcg gaggcgccgt cacacggcat gccgatcctg ctgtacgatg
     1441 tgaatagcgc aggcagtcag gcctacatgc gtctcgctgc agagatcatc gagcgcgagc
     1501 gccgctataa ggccgcgtga gcagaggcgg cgccgggtga cacaaggaga aaagaagcgt
     1561 catggcagct ccactccaga agggccggct tggcaggggt ctcgcatctc tcattggtga
     1621 tccggtgctt gcggaaccga agctgccatc cgagggcgag cagagatttg ttcccatcga
     1681 ccagattcgg ggcggcaagc tcaacccgcg gcgcgagttc aaggaggagg acctggccga
     1741 gctggcggag tcgattcgcc agaaggggct ggtgcaaccc atcgtcgtcc ggccggatcg
     1801 cctcgtcggt ggatacgagg tcgtcgccgg cgaacggcgt tggcgggcgg cgcagcgggt
     1861 cggcctccat tcgattccat gcatcgtacg cgagctctcg gaccaggagg tcctcgagct
     1921 cgggatcatc gagaacgtcc agcgggccga tctcaatccc atcgaggagg cggcgggcta
     1981 tcgtgagctc atcgaccgat tcggctatac gcaggaccaa ctcgccgatg tcatcggcaa
     2041 gagccgcagt catctcgcca acacgctacg cttgctcaag ctgccggaca cggttcggat
     2101 cctggtacag gaaggtaagc tgactgctgg tcacgcccgc gccctggtgg ggcgcgagga
     2161 cgcggaagcg cttgctgagc ggatcatcga tcgcgacatg aacgtgcgcg aggtggaggc
     2221 gctggtgcag tccggcggca ttggtcagcc gagccgtcgg cgcgacaaga acgccgacgt
     2281 actagcgttc gagaaggagc tctcggatgt tcttggcctc aaggtcgaga tcaagcgcgg
     2341 ctccggcgag agcggcgttc ttgccatcaa gtacggcaac tacgatcagc tcgattatat
     2401 ccggctgcgc ttgatcggct cgcaggtgga gtagggagcg gtcgcggggt tgctcgccga
     2461 gctggtctcg tcagtcgccg
//
LOCUS       DASSGX010000313         4239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1473223, whole genome shotgun sequence.
ACCESSION   DASSGX010000313 DASSGX010000000
VERSION     DASSGX010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473223"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VFI12_05510"
     CDS             complement(<1..372)
                     /locus_tag="VFI12_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055898.1"
                     /translation="MAALARPARERPLAAPLERARRLRRPLRLLREPRPEPHGRLDED
                     EQAHVRVLEAAELAAAAAVDADPVGVEEDPVALARDRVDLAVQLGHPEAVDHVRRRRH
                     EVDARADGDVDLVRRDRPRARI"
     gene            complement(556..1941)
                     /locus_tag="VFI12_05515"
     CDS             complement(556..1941)
                     /locus_tag="VFI12_05515"
                     /inference="COORDINATES: protein motif:HMM:NF033206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ScyD/ScyE family protein"
                     /protein_id="HET7055899.1"
                     /translation="MTRIWSLVFLALALVAPSAFAQSATPALSAVAGGLINPYGFAFG
                     AGGQLIVAETGTGGDQPAPEDVPAPAGPYLGGLTGRISRIDAGCPVPLAADLGSARAE
                     SGQTLGPSAVAVIGDTIYALLAGGGVAHANPDQPAGVYDVTSGSPALIADLGAWLRAN
                     PVVAPPAEGFDPDGQWVAMAAAPDGASLVVVESNSEQVVRVGLDGAIARIADLSAEDK
                     APSSVAIAPDGTIYVGNMSQPPYATGSASVIRIGSDGAAETVWSGLTLVSGIAIDGRG
                     TLYAAEFSAGRDRPPVIQPGTGRIIRQTGVDTVDEVATQLNLPTAIAFGPDGALFVSL
                     PLVGADAGTGVILRVDTSAELPLVAENFDLTPPSCGAPSDTVLIKVSDLGLDPASVTI
                     TAGMTVTWRNTGEFDHAVVSDPASPVQWDSGPLRPGEEFSQTFDQPGTYAYADGVFPD
                     HTGTIEVVSGG"
     gene            complement(1983..>4239)
                     /locus_tag="VFI12_05520"
     CDS             complement(1983..>4239)
                     /locus_tag="VFI12_05520"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET7055900.1"
                     /translation="LLIPIWVGERCLGILNLYSRKRNAFSNTQQTIAHTLATHAANAI
                     QNAQTHERERARRGQQEALLRLTAAATSSLNVHEAMLRVCMELCEVVDAESASIGILR
                     PELKAFEIVADVTAPGWDFADKPGDLDPIANYPSFERVIASSGAFMASREQGTPEQIR
                     FIENSPVGLETYVIASLRLNDACLGYLGIYDRRASQFNQETIELLDSASSLIALALSN
                     GLTARDQQRRAADRAAVVNVGRATISDASVAEKLQLISTACLDIHRVDGVSIYQWDAD
                     AGELTCMADTGREGWPYQLEIGYRFPVPADSPFRPFVEADDASSFTLLDQAMHPRLRE
                     DMEQSRGKASAIFPLRADDQLIGIACFYSLEPHPFDEEAIRVGKEVATQTALAIRASR
                     LLETTQRYANEQSAVLAVNRAVMDATQSSIDDVLAKISHETMVLISAECCEIECLLPD
                     LNATVLAAQVLVDDWRETASGPGLELPLNEWPITQRVLEQQQPIVLDHEVSGLSEREW
                     IGLFGFGTRSALIAPMVSGGRSVGTISFYSRVRHAFGPDEVRLAVEFGSLAALAIDRA
                     RTHMALQEQATIDGLTGVLNHRAFCDRLDHQMAIADRTEGPVSLLMIDLNDFKAVNDS
                     HGHLAGDAVLRETARFLAKVVRASDLVARYGGDEFAVILPGADAVDAIGLRNRLMEMA
                     PANRFTLADGSTVTPSFAIGWAAYPTQATDRQSLIDAADRSMYEAKHGTAGPMAASLE
                     LPAGDVVRLTG"
BASE COUNT          709 a         1395 c         1487 g          648 t
ORIGIN      
        1 tatccgggct cgcgggcgat cacggcgaac gagatccaca tccccgtccg cacgcgcgtc
       61 aacctcgtgg cgacgacggc ggacgtgatc cacagcttct gggtgcccga gctgaaccgc
      121 aagatcgaca cgatcccggg cgagcgcaac cggatcctcc tctacgccga ccgggtcggc
      181 gtctaccgcg gccgctgcag cgagttctgc ggcctccagc acgcgcacat gggcctgctc
      241 gtcttcgtcc agccggccgt gcggttccgg gcgtggctcg cgcaggaggc gaagcgggcg
      301 gcgaagccgg cgggcgcgct cgagcggcgc ggcgagcggg cgttcacgag cgggccgtgc
      361 cagagctgcc acacgatccg cggcacgagc gcggacggcg tcgtcggccc cgacctcacg
      421 cacgtcggcc ggcggacgac gctcgccgcc gacacgatcc cgaacacgcg cgaggacctc
      481 gccgcctgga tcacgggctc gcagcacgtg aagccgggga accagatgcc gaacctgcgc
      541 atcccggcgg ggacgctatc cgccggagac gacctcgatc gtgccggtgt gatccgggaa
      601 gacgccatcg gcgtaggcgt acgtgccggg ctggtcgaaa gtctgcgaga actcctcgcc
      661 ggggcgaagc gggccggaat cccactggac cgggctggcc ggatcggaga cgacggcgtg
      721 gtcgaattcg cccgtattgc gccaggtaac ggtcatgccg gcggtgatcg tgaccgaggc
      781 cggatccaga ccgaggtcgg agaccttgat cagcaccgtg tcgctcggcg cgccgcatga
      841 gggcggggtc aggtcgaagt tctcggcgac cagcggcagc tcggccgagg tgtcgacgcg
      901 gaggatgacg ccggtgccgg catcggcccc gaccagcggg agcgagacga agagggcgcc
      961 atccggacca aaggcaatgg cggtcggcag gttgagctgc gtggcgacct cgtcgacggt
     1021 gtcgacgccg gtctggcgga tgatccggcc ggtgccgggc tggatcaccg gcggacgatc
     1081 ccggccggcc gaaaattctg cggcatagag cgtgccccgg ccgtcgatcg cgatcccgga
     1141 aacgagtgtc aggccggacc agaccgtctc ggccgctccg tcggagccga tccgtatcac
     1201 cgaagcggaa ccggtcgcat agggcggctg gctcatgttg ccgacgtaga tcgtcccgtc
     1261 cggcgcgatc gcgaccgatg acggagcctt gtcctctgcg gagaggtcgg cgatgcgagc
     1321 gatcgcgccg tcgaggccga cccgcacgac ctgctcgctg ttgctctcga ccacgacgag
     1381 gctcgcaccg tccggcgcgg cggccatcgc gacccactgg ccgtcgggat cgaatccctc
     1441 ggccggcggc gccacaaccg ggttcgcgcg taaccaggcg ccgaggtccg cgatcaatgc
     1501 cgggctgccg gacgtgacgt cgtagacacc ggccggttga tccgggttgg catgggccac
     1561 gccaccgccg gcgagcagag cgtagatcgt gtctccgatc accgccactg cggatgggcc
     1621 gagcgtttgg ccactctcgg cccgcgccga gccgaggtcg gcggccagtg gcaccgggca
     1681 accagcatcg atccgggaga tacggccggt cagtccaccg aggtatggac cggccggggc
     1741 gggcacatcc tctggggcgg gttgatcccc gccagttccc gtctcggcca cgatcagttg
     1801 cccgccggcg ccgaaggcaa agccgtacgg gttgatcaga ccgccggcca ccgcggagag
     1861 cgccggtgtt gcggactggg caaatgcgga tggcgccact agcgccaatg cgaggaaaac
     1921 gagggaccag atacgcgtca tggatcctcc ttgcgcgggc gaacagccgg cgaaggaggc
     1981 cgctacccgg tcaggcgaac gacgtctcca gcaggcaatt cgagactggc agccatgggg
     2041 ccggcagtgc cgtgcttcgc ttcatacatg gaccggtcgg ctgcgtcgat cagtgactgc
     2101 cggtcggttg cctgggtagg atacgcggcc cagccgatcg cgaacgacgg ggttaccgtg
     2161 ctgccatcgg cgagcgtgaa ccgatttgcc ggggccatct ccataaggcg gttgcgcagt
     2221 ccgatcgcgt cgacggcatc ggcgccgggg agaatgaccg cgaactcgtc gccgccgtac
     2281 cgggcaacca ggtccgacgc ccggacgact ttcgccagaa accgggccgt ttctcgcaag
     2341 accgcatcgc cggccaggtg gccgtgcgaa tcgttgaccg ccttgaagtc gttcaggtcg
     2401 atcatgagca agctgacagg gccctcggtc cgatccgcga tggccatctg gtgatcgagc
     2461 cggtcgcaaa acgcgcggtg gttgagcacg ccagtcaacc cgtcgatggt cgcttgctcc
     2521 tgaagggcca tgtgcgtccg cgcgcggtcg atggcgagcg cggccaggct gccaaattcg
     2581 acggccagcc ggacctcgtc gggtccgaaa gcgtggcgca cgcgcgaata gaagctgatc
     2641 gtgccgaccg agcggccacc actcaccatc ggcgcgatca aggcggacct ggtgccgaat
     2701 ccgaacaggc cgatccactc ccgctcgctc aatccggata cttcatggtc gaggacgatc
     2761 ggctgttgct gctccagcac gcgctgggta atcggccact cgttgagcgg tagctccagc
     2821 cccgggccgg acgcggtctc ccgccagtca tcgacgagga cctgggccgc caggacggtc
     2881 gcattcaggt ccggcagcag gcactcgatc tcgcagcact cggcgctgat caacaccatc
     2941 gtctcgtgcg agatcttcgc cagcacgtcg tcgatcgagg actgcgtcgc atccatcacg
     3001 gcccggttga cggcgaggac ggccgactgt tcgttagcgt agcgctgggt cgtctcgagc
     3061 aagcgggaag cgcggatcgc gagggcggtc tgcgtggcga cctccttgcc gacgcggatg
     3121 gcctcctcgt cgaatgggtg cggctcgagc gagtagaagc aggcgatccc gatcaattga
     3181 tcgtcggccc ggagcgggaa gatcgccgat gccttaccgc gggactgctc catgtcctcg
     3241 cgcagccgcg ggtgcatggc ctggtcaagc agggtgaagg aggaggcgtc gtctgcttcc
     3301 acgaacggcc ggaaggggct atcggcgggg accgggaagc gatagccgat ctccaactga
     3361 tagggccaac cctcccgccc ggtatcggcc atgcaggtca actccccggc atcggcatcc
     3421 cactggtaga tcgagacacc gtcgacccgg tgaatgtcga ggcacgcagt gctgatcagt
     3481 tgcagtttct cagccacgct ggcatccgaa atcgtcgcgc ggccgacatt gacgaccgcc
     3541 gcgcggtcgg cggcgcgacg ctgctggtca cgcgcggtca ggccgttcga gagggcgagc
     3601 gcgatcaggg atgatgcgct gtccagcagc tcgatcgtct cctggttgaa ctggctcgca
     3661 cgccggtcat agataccgag atagccaagg cacgcgtcgt tcaagcgaag cgacgcgatc
     3721 acgtaggtct cgagtccgac cgggctgttc tcgatgaacc ggatctgctc cggcgtgccc
     3781 tgttcccggc tggccatgaa cgcgccgctg ctggctatca cccgctcaaa cgacgggtaa
     3841 ttggcgatcg ggtcaaggtc gccgggcttg tccgcgaagt cccaacccgg agctgtgacg
     3901 tcagcgacga tctcgaaggc cttcaactcg ggacggagga tgccgatcga cgcggattcg
     3961 gcgtccacga cctcgcacag ctccatgcag acgcgcagca tcgcctcgtg cacgttcagg
     4021 cttgacgttg cggccgcggt caaccgcagc agtgcttctt gctggccgcg gcgggcgcgt
     4081 tcgcgctcgt gcgtctgcgc attctgaatg gcgtttgcgg cgtgggtcgc cagggtgtgg
     4141 gcgatcgtct gctgcgtgtt ggagaaggcg ttgcgcttgc gggagtagag gttgaggata
     4201 cccaggcagc gctccccgac ccagatcggg atcagcaga
//
LOCUS       DASSGX010000314         4281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1036355, whole genome shotgun sequence.
ACCESSION   DASSGX010000314 DASSGX010000000
VERSION     DASSGX010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4281
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1036355"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1206)
                     /locus_tag="VFI12_05525"
     CDS             complement(<1..1206)
                     /locus_tag="VFI12_05525"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7055901.1"
                     /translation="MAAVTAAIHSRTEGLSREQLQFGFRVLAITTFLMTLALSSFDSI
                     QANFFRDSLGMDGELNGYLIAIREVPGFLLIFIAAMMLRQGLARATAVSIFIAGIGFS
                     LFAFTHSFSQAIIPILICSIGYHSWLQLQYALGLSLARNGEEGTTLGKLQGIGFLGTF
                     LALVLVWGALQTTEWLTGSDHQDRILRIFWIFAGAAAVIGAVAILRFPQSQRDRHEAK
                     VAPRLTWRREYWLYYVLATLDGSRQQIYFAFAPFVLVERFNVPARHLIIVLIIAALIK
                     WRMGEVIGKMVDQYGEKSTLNLAYVLHLIVFLGFALSPNVWFAYVSYLGYNFLSLFSI
                     GTTTYIKKIARREDLTPSLAMGVSLAHLTAIVVPVFGAALWDQLGYRFPFLFGTIFVI
                     LSLVFTQKID"
     gene            complement(1257..2522)
                     /locus_tag="VFI12_05530"
     CDS             complement(1257..2522)
                     /locus_tag="VFI12_05530"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7055902.1"
                     /translation="MTAAGKPRVVIIGGGFAGLNAAKQLKGADVEVTVVDQHNHHVFQ
                     PLLYQVATASLSPADIAGPIRGILRTQENLRVVMAKVTGVDLDQRIVQMGGDEMGYDY
                     LIIAAGATHSYFGHDEWEPYAGGLKTLDDAVRMRNQILLGFEAAERAHSTDERQRDLS
                     FAIVGGGPTGVELAGAIAEIARHSISRDFDNIDPRQARILLIEAGPRLLATFPESLSA
                     HAQVDLERLGVEVRLNSMVTGIDERGLTLASGERIEAGTKLWAAGVQASQLGKALGVD
                     LDRAGRVRVEPDLSIPGRPNVFVAGDLAALNGRDGKQLPGVAQVAMQEGRAAAKNILR
                     RIAHEPTKPFHYKDLGNMATIGRNRAVADIHGLRLHGYIAWIIWLFIHLINLIGFRNR
                     FIVLSQWIWGYLTFHRRVRLITKPGTTSD"
     gene            complement(2541..2831)
                     /locus_tag="VFI12_05535"
     CDS             complement(2541..2831)
                     /locus_tag="VFI12_05535"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HET7055903.1"
                     /translation="MTIGVAQITLYLEAADSLKDKRRVVKSLIQRIHNRFNVAAAEVA
                     DLDDHRVATLGVAVVSNSGAHCSDVLQAVIAFVDGNVELGSMGEVETELIPY"
     gene            complement(2828..3298)
                     /locus_tag="VFI12_05540"
     CDS             complement(2828..3298)
                     /locus_tag="VFI12_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055904.1"
                     /translation="MADGQHENDELEFGAEEQLELLRSNTLAMAYGTIAFLQEQGIPS
                     TAWVEALGDIFARGWDAEEQWTPEDFLDATILNLAAFGGEAIQAEFGDDEATALIVYF
                     PDPGRIAELGLDDVDGDLLYELITPIARVCGVRYRWQRDGEHVRIRIRPAAAAR"
     gene            3550..>4281
                     /locus_tag="VFI12_05545"
     CDS             3550..>4281
                     /locus_tag="VFI12_05545"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7055905.1"
                     /translation="MPANTYDVVIIGGGHNGLVAACYLAKAGFSVCVLERYHEVGGAA
                     FTQEIHPGYLLSEGSYVLSLMPRKIIDELGVTDDIQLVKRNPRFFMPFPDGQSLTAWE
                     DDQRFLDEIGKFSKKDAANYPHYDAFVEKASEVMDQFILRPPPTFAEFANQFKTTEEA
                     RIFQKMILGSAANVAEYFFESEQIQANAVAFGLIGTFRGPRDAGTAYVKLYHAMGQVT
                     GRRGQWAYVKGAMGAVSQAYKRVALA"
BASE COUNT          784 a         1412 c         1354 g          731 t
ORIGIN      
        1 gtcgatcttc tgggtgaaga ccagcgacag gattacaaag atcgtcccga acaggaacgg
       61 aaagcggtag ccgagctgat cccagagcgc cgcgccgaag accggcacga cgatcgccgt
      121 caaatgcgcg agcgagacgc ccattgccag cgacggcgtc agatcctccc gccgggcgat
      181 cttcttgata taggtcgtcg tcccgatgct gaacagcgac aggaagttgt acccgaggta
      241 gctgacgtaa gcgaaccata cgttcggcga gagtgcgaag ccgaggaata cgatcaggtg
      301 gaggacgtag gcgaggttca acgtcgactt ctcgccgtac tgatcgacca tcttgccgat
      361 cacctcaccc atccgccact tgatgagcgc cgcgataatc aggacgatga tcaggtggcg
      421 cgccggcacg ttgaaccgct cgaccagcac gaagggggca aaggcgaaat agatctgctg
      481 ccggctgcca tcgagcgtgg ccagcacgta gtagagccag tattcgcgcc gccaggttag
      541 cctgggcgcg actttcgcct catggcggtc ccgctgcgac tgtgggaagc ggaggatcgc
      601 caccgcgccg atgacggccg cggcgcccgc gaagatccag aagatgcgca ggatccggtc
      661 ctggtggtcg ctgcccgtca gccattcggt ggtctgcagc gcgccccaga ccagcacgag
      721 cgcaaggaac gtgccgagga agccgatccc ctgcagcttg cccagcgtgg tgccctcctc
      781 gccgttccgg gcaagtgaca gaccgagcgc gtactggagt tgcagccacg agtggtagcc
      841 gatggagcag atcaggatcg ggatgatcgc ctggctgaac gaatgggtga aggcgaacag
      901 cgagaagccg atcccggcga tgaagatcga gaccgccgtc gcccgggcca atccctgccg
      961 gagcatcatg gcggcgatga agatcagcag gaaaccgggc acctcgcgga tcgcgatcag
     1021 gtagccgttg agctcgccgt ccatgcccag gctgtcgcgg aagaagttcg cctggatcga
     1081 atcgaacgag ctgagggcaa gcgtcatcag gaacgtggtg atcgccagca cccggaagcc
     1141 gaattggagt tgttcgcgtg aaagaccttc cgtgcgggaa tgaatcgccg ccgtaaccgc
     1201 tgccaccagt gtccctgacc ctgccctaac ccgatacctc gtaccgggat tctacgctag
     1261 tcggaggtgg tgcccggctt ggtgatcagg cggacgcggc ggtggaaggt gaggtagccc
     1321 cagatccatt gcgacaggac gatgaacctg ttccggaagc cgatcaggtt gatcaggtgg
     1381 atgaagagcc agatgatcca ggcgatgtat ccgtgcagcc ggagcccgtg gatatccgcc
     1441 accgcccggt tccgcccgat cgtcgccatg ttgccgaggt ccttgtagtg gaacggcttc
     1501 gtcggctcat gcgctatccg gcggaggatg ttcttcgccg cggcccggcc ctcctgcatc
     1561 gccacctgcg cgacgcccgg aagctgcttc ccgtccctcc cattcaacgc ggcgaggtcg
     1621 cccgccacga acacgtttgg acgaccggga atcgagagat ccggctcgac ccggaccctc
     1681 ccggcccggt cgagatcgac gcccagcgcc ttgccgagct gcgacgcctg cacgccggct
     1741 gcccaaagct tggtgcccgc ctcgattcgc tccccgctgg ccagggtcag gccgcgctcg
     1801 tcgatgccgg tcaccatcga gttgagccgg acctccactc ccaaccgctc gaggtccact
     1861 tgggcatgtg cggatagcga ctccgggaag gtcgcaagca ggcgcggccc ggcttcgatc
     1921 aggaggatcc gcgcctggcg gggatcgatg ttgtcgaagt cccgcgagat gctgtgccgg
     1981 gcgatttcgg caattgcgcc ggccagctcg acgccggtcg gaccaccgcc gacgatcgcg
     2041 aagctcaggt cgcgctgtcg ctcatcagtc gagtgcgcgc gttcggccgc ctcgaaaccg
     2101 agcaggatct ggttccgcat ccggaccgca tcgtcgagcg tcttgagacc gcccgcgtac
     2161 ggctcccact cgtcgtgtcc gaagtaggag tgcgtcgccc cggccgcgat gatcaggtag
     2221 tcgtagccca tttcgtcccc gcccatctgg acaatgcgtt gatccaggtc gacgccggtc
     2281 accttcgcca tgaccactcg cagattctcc tgcgtccgga gaatgccccg aattggcccg
     2341 gcgatgtcgg ccggcgagag cgacgccgtc gcgacctggt agaggagcgg ttggaagacg
     2401 tgatgattgt gctggtcgac gaccgtaacc tcgacgtcgg cgcccttgag ctgcttggcc
     2461 gcgttcaacc ccgcgaaccc gccgccgatg atcacgaccc gtggcttgcc cgccgccgtc
     2521 atgtccgccc tcccttgcgc ttaatacggg atcagctccg tttccacctc gcccattgat
     2581 ccaagctcga cattgccatc gacgaacgcg attacggcct ggagcacgtc gctgcaatgc
     2641 gcgccgctgt tgctgaccac ggccacacca agcgtcgcga cccgatgatc gtcgagatcg
     2701 gctacctccg ccgccgcgac gttgaaccgg ttatggatcc gctggatgag cgatttgacc
     2761 actcgccgtt tgtccttgag cgagtcggcc gcctccaggt agagcgtgat ctgcgcgacc
     2821 ccgatcgtca tcgtgctgcg gccgccgggc gaatccggat ccgcacatgc tcgccatccc
     2881 gctgccagcg ataccgcacc ccgcagaccc gggcaatcgg cgtgatcagc tcgtagagga
     2941 ggtcgccgtc gacgtcatcg aggccaagct cggcgatgcg gccgggatcg gggaagtaga
     3001 cgatcagcgc ggtcgcctcg tcgtcgccga actcagcctg gatcgcctcg ccgccgaatg
     3061 cggccaggtt gaggatcgtg gcatccagga aatcctcggg cgtccactgc tcctcggcat
     3121 cccagccccg ggcgaagata tctccgagcg cctcgaccca ggccgtcgag ggaatcccct
     3181 gttcctggag gaacgcaatg gtcccgtaag ccatggcgag cgtgttgctc cgcaggagtt
     3241 ccagttgctc ctccgcgccg aactccagct cgtcgttctc atgctggccg tctgccacga
     3301 tgccctccgc tcatgactca aatatgcaat acaaatgtag ccccgggcgg cccgcccggc
     3361 cccgttggag aaacgccggg cgagccgccc ggggctacct caatgggcgg gctccggact
     3421 cgagcattgt gtcgcccacc agacagtatc ggatagcggc gtggacaggg gcgagattgc
     3481 gccgttgcga ccgaatcggt aggctgcagc agtcgggaca ccactggact gtcgggaaag
     3541 gactgccgcg tgcctgccaa tacctatgac gtcgtcatta tcggcggtgg ccacaacggg
     3601 ctggtcgcgg cgtgttacct ggcgaaggcc ggcttctcgg tctgcgtgct ggaacggtat
     3661 cacgaggtcg gcggcgccgc cttcacgcag gaaatccacc ccggctacct gctctccgaa
     3721 ggctcgtacg tcctctccct gatgccgcgc aagatcattg atgagctcgg cgtgaccgac
     3781 gacatccagc tcgtcaagcg caacccgcgc ttcttcatgc ccttccccga tggccaatcg
     3841 ctgaccgcct gggaggacga ccagaggttc ctcgacgaga tcggcaagtt ctcgaagaag
     3901 gacgccgcga actacccgca ctacgacgcc ttcgtcgaga aggcttcgga agtgatggat
     3961 cagttcatcc tccgtccgcc gccgaccttc gccgagttcg cgaaccagtt caagacgact
     4021 gaggaagcgc ggatcttcca gaagatgatc ctcggctcgg ctgcgaacgt tgccgagtac
     4081 tttttcgaga gcgaacagat ccaggcgaac gccgtcgcgt tcggcctcat cggcaccttc
     4141 cgcgggccgc gcgatgccgg tacagcctat gtcaagctct accacgcgat ggggcaggtc
     4201 accggccggc gcggtcagtg ggcctacgtc aaaggcgcga tgggcgctgt cagccaggcc
     4261 tacaagcgag tcgcgctcgc g
//
LOCUS       DASSGX010000315         3774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_819059, whole genome shotgun sequence.
ACCESSION   DASSGX010000315 DASSGX010000000
VERSION     DASSGX010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819059"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..956)
                     /locus_tag="VFI12_05550"
     CDS             complement(45..956)
                     /locus_tag="VFI12_05550"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET7055906.1"
                     /translation="MAGKRPLRIGLYIPNGTGYMANGVHRWSDILAYAQAAEAVGFDS
                     VWVADHMLFRFPGEETQSRWECWSVLSAIAAVTTTVELGPLVSCMSFRNPAHLAKITE
                     TVDEISNGRVILAVGAGWHEPEYTAYGFPFDHRASRFEEGFQIVHDLLRTGSSDFSGR
                     FYSVDDCEVTPRGPRPNTIPLIGGTNGERLLRITARLADGWNTDWIGTPDAVPALREA
                     VDRACELEGRDPATLSRSACAFIDMPNASGRFPKADPRAPGPQSHEQIIETLHRYAEE
                     GLSEVMVWLDPNTIEAIEEFGSLMARL"
     gene            1071..2198
                     /locus_tag="VFI12_05555"
     CDS             1071..2198
                     /locus_tag="VFI12_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055907.1"
                     /translation="MDFVVVGIVIGAIIVGAGAVVRDLGPRRRPFIDRRIRREISPEA
                     KAKAWRRFCQSLGTFVLTMGMIVILATVVAMVLSLSDSTGWLMVGASSALALVASGIG
                     VLVIPGHYQRGGFDPVIKQVTVRPAKAPPPVVMHDVEVPLSELPGDSIDDLFSLDVGL
                     EMPEPVPAPVEHMPAPAESVPIDDGITLPGTVFAEPEEPVDILPLTREPELTSPAAAE
                     PHAERRSARRVTFSRPNTTRSDPEQTPSPARPVREPDPMPPVYETPLIDPDEDLPAWN
                     AQPKPIPQPQPYVPPARVERPQSDPETGFQSSLFSDLSASGEEGTDVTGPFQSRLLNE
                     LTRNERRPEPEPEGGGADVLIDEVSLPVRNPNEQRNANNGR"
     gene            2179..3255
                     /locus_tag="VFI12_05560"
     CDS             2179..3255
                     /locus_tag="VFI12_05560"
                     /inference="COORDINATES: protein motif:HMM:NF020318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1786 domain-containing protein"
                     /protein_id="HET7055908.1"
                     /translation="MPTTVANPLGRTARSDTILAIDIGAGTQDILVYDPAQTPENCFR
                     LVMPSRTQIVGRQIREVTEAGRPLHLVGHVMGGGASTSAIRHHLEAGLPVTATPAAAR
                     TIHNDPQRVRHHGVWITDEPPRGAVEIELGDIDLAAIETALHSFGVDLPERYAIAVQD
                     HGYRTGHGNNDVRGDYLHWLVNSAGHLHRAVFLTPPEEMTRMQAVHLLAPGAIVMDTG
                     AAAVLGAMRDPVVAQAAEEGAVLVNLGNMHTFATLVRQGRLLGVFEHHTGGLDHDLLG
                     RLVERLRYGELTNREFRERYDGHGAVIAEDYRAFSPFRFVAVTGPNRGIAEGLNWHQA
                     APHGDMMLTGAFGLVDGYLASEGG"
     gene            complement(3357..3668)
                     /locus_tag="VFI12_05565"
     CDS             complement(3357..3668)
                     /locus_tag="VFI12_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055909.1"
                     /translation="MFRLATVPQFAGRRFARPALISRPKARACVAEKAAHASTADIRI
                     RRFADASVNEDTYSAILPNEGTSPVDLLRAITEAMQDLPEFQIQGAEDERFVLTLTLK
                     A"
BASE COUNT          610 a         1303 c         1236 g          625 t
ORIGIN      
        1 tcccgggcac cgccgatacg aaacttccct tcccgaaagc ccgctcacaa ccgcgccatc
       61 aatgacccga actcctcaat cgcctcaatc gtgttcgggt caagccagac catcacctcg
      121 ctcaacccct cttccgcgta ccggtgcaag gtctcgatga tctgctcgtg cgactgcggc
      181 ccgggcgcgc gcggatccgc cttcgggaag cggccggacg cgttcggcat atcaatgaag
      241 gcgcacgcgc tccgggacaa cgtcgccgga tcccgcccct ccagctcgca ggctcgatcc
      301 accgcctcgc gcagcgctgg cacggcgtcg ggcgtgccga tccagtcggt gttccagcca
      361 tcagccagcc gcgccgtgat ccgcaggagc cgctcgccgt tcgtcccgcc gatcagcggg
      421 atggtgttcg gccgcggacc gcgcggcgtc acctcgcagt catccaccga atagaagcgg
      481 cccgagaagt cgctgctgcc ggtgcggagc agatcgtgga cgatctggaa cccctcctcg
      541 aaccggctcg cccggtgatc gaacgggaag ccgtaggccg tgtactcggg ctcgtgccag
      601 cccgctccga ccgccaggat cacccgcccg ttgctgatct catcgacggt ttcggtgatc
      661 ttcgccaggt gcgccgggtt gcggaagctc atgcagctca ccagcggacc aagctcgacc
      721 gtggtggtga ccgccgcgat cgccgagagg acgctccagc attcccagcg gctctgcgtc
      781 tcctcgccgg ggaagcggaa gagcatgtgg tcggccaccc agaccgaatc gaacccgacc
      841 gcctccgccg cctgcgcgta ggcgaggatg tcgctccacc gatgaacgcc gttcgccatg
      901 tacccggtgc cgttcgggat gtacagcccg atccgcaatg gacgcttgcc agccacgtct
      961 tccctttcct tgtcactcgt tccggtctcc agttgcgagg agtagaatag ccaaccgggg
     1021 aagcaactga tcggcgccat gctgaccggt caccgggggg acaggggtcc gtggatttcg
     1081 tcgttgtcgg catagtaatt ggggcgatca tcgtcggcgc cggcgccgtc gttcgtgatc
     1141 tcggaccccg ccgccggccc ttcatcgacc gtcgcattcg ccgggagatc tcgcccgagg
     1201 cgaaggccaa ggcgtggcgc cgcttttgcc agtcgctcgg cacgttcgtc ctgactatgg
     1261 gcatgatcgt catcctcgcg accgtcgtgg ccatggtcct cagcctgtcc gactcgaccg
     1321 gctggttgat ggtcggcgca tcctccgccc tcgccctggt cgcgtccggg atcggcgtcc
     1381 tggtcatccc cggccactac cagcggggcg gtttcgatcc cgtcatcaag caggtgacgg
     1441 tccggccggc caaggcgccg ccgcccgtcg tcatgcatga cgtcgaggtg ccactcagtg
     1501 agttgccggg cgattcgatc gacgacctct tctcgctcga cgtggggctc gagatgcccg
     1561 agcccgttcc tgccccggtc gagcacatgc cggcgccagc cgaatcggtc ccgattgacg
     1621 atgggataac gctgcccggg acggtgttcg cggagccgga ggagcccgtc gatatcctgc
     1681 cactgacccg ggaaccagag ctgacgtcgc cggccgccgc cgaaccgcac gcggagcggc
     1741 ggtcagcacg gcgggtcacc ttctcgcgcc cgaacacaac ccggtccgac cctgagcaga
     1801 cgccgtctcc ggcacggccg gtccgcgaac cggaccccat gccgccggtg tatgagacgc
     1861 cgctgatcga tccggacgaa gatttgccgg cctggaatgc gcagccgaaa cccattccgc
     1921 aaccgcaacc atacgttccc ccggcccgcg tcgagcgacc gcaatccgat ccggaaaccg
     1981 ggttccagtc gagcctcttc tccgatctca gcgcctcagg cgaggagggg accgatgtta
     2041 ccgggccgtt ccaatcgcgg ctcctcaacg agctgacgcg aaacgaacgg cgaccagagc
     2101 cggagccgga aggcggcgga gccgatgttc tgatcgacga ggtgtcgctc ccggtccgca
     2161 atccgaacga gcagcgcaat gccaacaacg gtcgctaacc cgctcggccg caccgcccgc
     2221 tctgacacca tcctggcgat cgatatcggc gccgggacgc aggacatcct ggtctacgac
     2281 ccggcccaga cgcccgagaa ttgcttccgg ctggtcatgc cgtcccggac tcagatcgtt
     2341 ggccggcaaa tccgcgaggt gaccgaggcc ggccggccgc tccacctcgt cgggcacgtc
     2401 atgggtggcg gcgcatcgac ctcggcgatc cgtcaccatc tcgaagccgg gttgccggtg
     2461 accgccacgc cggcggccgc ccgcacgatc cacaacgacc cgcagcgtgt ccgtcaccac
     2521 ggcgtctgga ttaccgacga gcccccgcgc ggcgcggtcg agatcgagct gggcgacatt
     2581 gacctcgctg caatcgaaac ggccctgcat tcgttcggcg tcgacctccc ggagcggtat
     2641 gcgatcgcgg tgcaggacca cggctaccgg accggtcacg gcaacaacga tgtccggggc
     2701 gactacctgc actggctggt caattccgcc ggccatcttc atcgcgcggt cttcctgacc
     2761 ccgcccgaag agatgacccg gatgcaggcg gtccacctgc tcgctccggg ggcgatcgta
     2821 atggacaccg gcgcggcggc tgtcctcggc gcgatgcgcg accccgttgt ggcccaggcc
     2881 gccgaggagg gagccgtgct ggtcaacctc ggcaatatgc acaccttcgc cacgctcgtc
     2941 cggcagggac ggttgctcgg cgttttcgag caccacaccg gcggtctgga tcatgatttg
     3001 ctggggcggc tggtcgagcg gctgcgctat ggcgagttga ccaaccggga gttccgggag
     3061 cggtatgacg gccacggcgc ggtgatcgcc gaggattacc gggcattctc gccgttcagg
     3121 tttgtggcgg tcaccggacc gaatcgcggg attgccgagg gtctgaactg gcatcaggcc
     3181 gcgccacacg gagacatgat gctgaccggc gcgttcgggt tggtcgacgg gtacctggcg
     3241 tccgagggcg gctgagcgcg gggcgggaac ggtgcggggt ccgtcccggt gggatgcggg
     3301 gagtgcgggt gcgggcgggg cggcagggat cgccccggcg aggtgcggga gcgggatcag
     3361 gccttcaggg tcagcgtcag gacgaagcgc tcgtcctcgg cgccctggat ctgaaactcg
     3421 ggcagatcct gcatcgcctc ggtgatcgcg cgaagcagat cgaccggcga ggtgccctca
     3481 tttggcaaga tcgccgagta cgtatcctcg ttgaccgagg catccgcaaa gcggcgaatg
     3541 cggatgtcgg ccgtggaggc gtgcgcggcc ttctcggcga cacaagcgcg cgctttgggt
     3601 cggctgatca gcgccggcct ggcgaagcgg cgaccggcga actgggggac ggttgcgagt
     3661 cggaacatca ctgtctcctg ctagtacagt cgtttcgaac aactgtacgt acagtatcga
     3721 acacttgttc ctttgtcaag taggcaacac cacgccactt cagttcctgc tggt
//
LOCUS       DASSGX010000316         3812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_491800, whole genome shotgun sequence.
ACCESSION   DASSGX010000316 DASSGX010000000
VERSION     DASSGX010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491800"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1385)
                     /locus_tag="VFI12_05570"
     CDS             complement(<1..1385)
                     /locus_tag="VFI12_05570"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7055910.1"
                     /translation="MASAQSSPAALRPTQTDAVLPRLTRLELIGFKSFAAKTLFQFEP
                     GITAVVGPNGSGKSNIADAVRWALGETSYSSLRSKKTEDVIFAGGAGKAPAGMAEVTL
                     TFDNATGWLPIDFTEVTVTRRAFRSGETAYLINGRKVRLKDIHQLTASLGQSYTVVGQ
                     GLVDAALSQRAEERRGLFEHAADLTGLRLKAAEAERNLAETGANTARLTDLLAEVEPR
                     LRSLERAARQANEYRDVRDRLRMLQRGHFRQLLLDVSERLAAVEQAAATGQSAAESIQ
                     NRHDELVARAAVLRSERDQAGDVLAQHRAGSQATAEQLRRVMHEREIVSERLASLIRR
                     REDMTETQQGLEEQIASVLANLEDVRTQLAAADAAAGSARQERDRADAAMAAERAGRV
                     AVEREHARLLALVTDRERQIADASRRKALLVQRAETDAAERERAEQTSGDRRERHQYL
                     TADLTALDQSE"
     gene            1626..1925
                     /locus_tag="VFI12_05575"
     CDS             1626..1925
                     /locus_tag="VFI12_05575"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HET7055911.1"
                     /translation="MASSKHRTAFIIGALLGASAGVAAAFLNAPQSGRRTRDQIQQAA
                     ERVLFKALDMVPFQNQPTDRLATSYPVDEVVIAAEPPVDIVIGSRPSDMGMTHTP"
     gene            1922..2068
                     /locus_tag="VFI12_05580"
     CDS             1922..2068
                     /locus_tag="VFI12_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055912.1"
                     /translation="MIDKAQAKMKFFVYGMLLGLAFAPYSGKENRSRVMGWFAGGIRD
                     VVKF"
     gene            2087..2806
                     /locus_tag="VFI12_05585"
     CDS             2087..2806
                     /locus_tag="VFI12_05585"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET7055913.1"
                     /translation="MFPIGDENRGIRSRAYVNYALIAVNILVFLYQLTLSERDLARFF
                     IDWGVVPWNISHGERLATLVTSQFMHGGWLHIGGNMLFLWVFGDNIEDTMGHIGYLIF
                     YLLCGVAAGLAQVLVNTDSRIPQIGASGAISGVLAAYLVLFPRGKVRTLLFVGIPFII
                     LIPAWGMIGYWIVIQFINGVATLGVKTQQTSGVAYFAHIGGFIAGLVLVWVFRDRETH
                     QRQLEMRAGNRPFERTRLLGR"
     gene            complement(3193..>3812)
                     /locus_tag="VFI12_05590"
     CDS             complement(3193..>3812)
                     /locus_tag="VFI12_05590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055914.1"
                     /translation="RWDGKEVLELPSLGGSNNRATGINDAGIIVGYASQDETGDRVHA
                     VRWVEGAVEDLGTLGGEIGQAMAINRHGQIVGSSTSEKGFSAVDHAFVWADGEMTRLG
                     RLKSVTIHGRSGKIKLDRSVSVGINDAGVICGFSVSASENDPVSVATLWDGDEVLDLN
                     SAIGKANRDLVLSSADGINRDRDLVCTGYLLDDPQTPRLFRLEPA"
BASE COUNT          598 a         1230 c         1298 g          686 t
ORIGIN      
        1 tcttcggatt ggtcgagcgc ggtcaggtcg gccgtcagat actggtgacg ctcgcgtcga
       61 tccccggatg tctgctccgc gcgctcccgc tcggcggcgt cggtctcggc gcgctgaacc
      121 agcagggcct tgcgccggga ggcatccgca atctgccgtt cacggtcggt gacgagcgcc
      181 aggagccgcg cgtgctctcg ctcgacggcg acccggccgg cacgttcggc ggccatggcg
      241 gcgtcggccc ggtcccgctc ctgccgggcg gacccggcgg cggcatcggc ggcggcaagt
      301 tgcgtccgga cgtcctcgag attcgccagg accgacgcga tctgctcctc gaggccctgc
      361 tgcgtctcgg tcatgtcctc gcggcgccgg atgagtgacg ccaatcgctc gctgacgatc
      421 tcccgctcgt gcatgacccg gcgcaattgc tcggcggtcg cctgcgaccc ggcgcggtgc
      481 tgcgcgagca catcgccggc ctggtcacgc tcggaacgca ggactgcggc gcgggcaacg
      541 agctcatcgt gccggttctg gatcgattct gctgccgact ggccggtggc ggccgcctgc
      601 tcgaccgccg ccagccgctc actgacatcg agcagtagct ggcggaagtg gccgcgctgg
      661 agcatccgca gccggtcccg gacatcccgg tattcgtttg cctgccgggc ggcccgctcg
      721 agcgagcgca ggcgcggctc gacctcggcc agcaggtcgg tcaagcgggc ggtgttggcc
      781 ccggtttcgg ccagattgcg ctcggcctcg gccgccttga ggcgcaggcc ggtcaggtcg
      841 gccgcgtgct cgaagaggcc gcgccgctct tccgcgcgct ggctcagggc agcatcgacc
      901 agcccctggc cgacgacggt gtagctctgg ccgagcgacg cggtgagctg gtgaatgtcc
      961 ttgaggcgaa ccttgcgacc gttgatcagg taggcggtct cgccgctgcg gaaggcgcgg
     1021 cgggtgacgg tgacctcggt gaaatcgatt ggcagccaac cggtcgcgtt gtcgaaggtg
     1081 agcgtgacct cggccatccc ggccggagcc ttgcccgcgc cgccggcgaa gatgacatcc
     1141 tcggtcttct tgctgcggag cgagctgtag ctggtttcgc cgagggccca gcggaccgca
     1201 tcggcgatgt tcgacttgcc gctgccgttc gggccgacga cggcggtgat gcccggctcg
     1261 aactggaaga gggtctttgc ggcgaagctt ttaaagccga tcagctcgag gcgggtcagg
     1321 cgcggcaata cggcatcggt ctgggttggc cgcaaggcgg ccgggctcga ttgagcggat
     1381 gccatggggt tcgttgatcc tcgagcggac tggtctgcgc ggcacaggca acattcaccg
     1441 agtcatgcga atcggctctc atgcgcagcg ccactgttcg tgcgcatatg gtacagccgg
     1501 aggggttcga acgccgaatt gtcgccggga atcagcgatt ttttggccga acgattaggc
     1561 tggctggtag gatgcgcgtg gtgaccagat gacaccgccg atttcatacc ccggagagca
     1621 cgtctatggc ttcatccaag catcggaccg cgttcatcat cggggcgctg ctcggcgcca
     1681 gcgccggtgt ggcggccgca ttcctgaacg ctccgcaatc cggccggcgg acgcgggatc
     1741 agatccagca ggcggcggag cgggtgctgt tcaaggcgct ggacatggtc ccgttccaga
     1801 accagccgac cgatcgattg gccacgagtt atccggtcga cgaggtcgtg atcgccgcgg
     1861 aaccgcccgt ggatatcgtg attggcagcc ggccgagtga tatggggatg acgcacacgc
     1921 catgatcgac aaagcccagg ccaaaatgaa gttcttcgtc tacggcatgc tgctcggact
     1981 cgccttcgcg ccctacagcg gcaaggaaaa ccgctcccgc gtgatggggt ggttcgcggg
     2041 cggcatccgc gacgtcgtca agttctagcg gccgacccaa agacgcatgt tcccgatcgg
     2101 cgatgagaat cgcggcattc gctcgcgtgc ctacgttaac tatgccctga tcgcggtcaa
     2161 catcctcgtc ttcctgtatc aactgacgct ctccgagcgg gacctggccc ggttcttcat
     2221 cgactggggc gtggtgccgt ggaacatcag ccacggcgag cggctggcca cgctggtcac
     2281 ctcgcagttc atgcacggcg gctggctgca catcggcggc aacatgctgt tcctctgggt
     2341 cttcggggac aacatcgagg acacgatggg ccacattggc tacctgatct tttacctgct
     2401 ctgcggggtg gcggctgggc tggcgcaggt gctggtcaac accgacagcc ggataccgca
     2461 gatcggcgca tcgggcgcga tctcgggcgt cctcgccgca tacctcgtgc tcttcccacg
     2521 cggcaaggtc cggacgctgc tgtttgtcgg cattcccttc atcatcctga tcccggcgtg
     2581 gggaatgatc ggctactgga tcgtcatcca gttcatcaat ggggttgcga cattaggcgt
     2641 gaagacacag cagaccagcg gcgtcgccta tttcgcgcac atcggcggat tcattgccgg
     2701 gctggtattg gtctgggtct ttcgggaccg cgagacgcac cagcgccaac tcgagatgcg
     2761 agccgggaat cggccgtttg agcgcacgcg gttgctcggc cggtgaagcc gcccccgatt
     2821 tccaatggat ctccccggcg tcgcccgggg ataaatgccc gggctgagga acgacaagcc
     2881 cgctaaaggg gctgaccagg ggttccaacc gggagagggg cattccggcg ccgccccaga
     2941 ttgaaatcca gggccagggt acggatgtcg gttgaaaccg attttttcag tccctttgga
     3001 gcgggtttca gaagaagtgc caccagccca atgtagcccc gggcgccgcc ggacagccgg
     3061 tgggacagtc ggagtccgtc aacaggcagt gatcaacgcc ctacaggcga tcgccgggca
     3121 catcccctct ccaacgctcc ccgccgcggg agagggcatt acccgcgcca cgttcctgga
     3181 cgcctgcaaa tttcaggcgg gttcgaggcg gaagaggcgg ggcgtttgcg ggtcgtcgag
     3241 caggtagccg gtgcagacga ggtcgcgatc ccggttgatg ccgtcggcgc tggagaggac
     3301 gaggtcccgg ttggccttgc cgatcgcgct gttgagatcg agcacttcgt cgccgtccca
     3361 gagggtggcg accgagaccg ggtcgttctc ggatgcgctg accgagaagc cgcagatcac
     3421 gcccgcgtcg ttgatcccga ccgagaccga gcgatcgagc ttgatcttgc ccgaacggcc
     3481 gtggatcgtg accgacttga gccggcccag gcgggtcatc tcaccgtcgg cccagacaaa
     3541 ggcgtgatcg accgcgctga accccttctc gctggtcgat gagccgacga tctggccgtg
     3601 ccggttgatc gccattgcct ggccaatctc cccgccgagc gtgccgagat cctcgaccgc
     3661 accctcgacc cagcggacag catggacccg gtcgccggtt tcgtcctggc tggcatagcc
     3721 gacgatgatc ccggcgtcgt tgatcccggt cgcgcgattg ttcgatccgc cgagcgaggg
     3781 gagttcgagc acctctttgc catcccagcg ca
//
LOCUS       DASSGX010000317         2258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1473711, whole genome shotgun sequence.
ACCESSION   DASSGX010000317 DASSGX010000000
VERSION     DASSGX010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473711"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="VFI12_05595"
     CDS             complement(<1..472)
                     /locus_tag="VFI12_05595"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7055915.1"
                     /translation="MPAQDGTGMIATLRQRNFALLWTAGLVSLTGDWAFYAAMPIFIL
                     DRTGSAFQSGLVWAAIALPGIFIGPFAGVYVDRLDRRRIMLFGNIAQAAAASILLLIG
                     GAEGVWIAMAVLLIQSSLYAVYSPAESALLPTLVDDQHLPTANALNALNDNIARI"
     gene            complement(582..1154)
                     /locus_tag="VFI12_05600"
     CDS             complement(582..1154)
                     /locus_tag="VFI12_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055916.1"
                     /translation="MSSATDSLPRRERTRLWYRAESHTALEDHLISEQPSGEHVRGTG
                     RLPRTGQLRALEALQATLNQDDAEASATETIEITEALTDWRDSPRHCPVLLLSASPLL
                     DARNPGPLLGPGTRYRIVTVEAGLVCLQVDALDGRVGVGYCNAVDLMCYEPEIMYLRH
                     QKRTGRLQTAKLSLSRLSQRITGGTGSLSS"
     gene            1371..1733
                     /locus_tag="VFI12_05605"
     CDS             1371..1733
                     /locus_tag="VFI12_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055917.1"
                     /translation="MDERDRTAQPRQRTISKLPFDQRAIGEIAALLGATPSLAPFQLP
                     GGEVHQLTVQRDEAPPSVLLTLWPTIRRVDAISQSATVVFTNVVGVELVEGVEVLFRR
                     GTGEYLIVTVAGKVIVRA"
     gene            complement(2075..>2258)
                     /locus_tag="VFI12_05610"
     CDS             complement(2075..>2258)
                     /locus_tag="VFI12_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055918.1"
                     /translation="LGDACTRAGIQPGLPFTQRRVWVLPRLFRGKSVGWQNTIAQAAA
                     VIVLGISLAGFLTLQR"
BASE COUNT          381 a          678 c          821 g          378 t
ORIGIN      
        1 caattcgcgc gatgttgtcg ttgagcgcgt tgagggcgtt ggcggttgga agatgctggt
       61 cgtcgacgag cgtcggcagc agcgcgctct cggccggtga atagaccgcg tagaggctgg
      121 actggatcag gagcacggcc atcgcgatcc agacgccctc ggccccgccg atcaggagca
      181 ggatgcttgc tgctgcggct tgggcaatgt tgccgaagag catgattcgc cggcgatcga
      241 gccgatcgac atagacgccg gcgaacgggc cgatgaagat gcccggcagc gcgatcgcgg
      301 cccacacgag gccggattga aaggccgagc cggttcgatc caggatgaag atcggcatcg
      361 ccgcgtagaa ggcccagtcg ccggtcagcg agacgagccc ggcggtccag agcagcgcga
      421 aattccgttg acgaagcgtg gcgatcatcc cggtcccgtc ttgcgctggc acagcggcca
      481 agtttacccg gggatcggcc ggggcacacc aacggcggcg gtgcgccggg ggagagcgca
      541 cgtgccgccg ttggtgacgg gagagttgga tgcgggagcg gttagctgct cagcgagccg
      601 gtgccgccgg tgatccgctg cgagagccgg ctgagactga gcttggcggt ctgaagccgg
      661 ccggtgcgct tctgatggcg caggtacatg atttccggtt cgtagcacat gagatcgacc
      721 gcgttgcagt agccgacgcc gacgcggcca tccagcgcgt cgacctggag acagacgagc
      781 cccgcctcga ccgtgacgat gcgatatcgc gtcccgggac cgaggagcgg acccggattg
      841 cgggcgtcaa ggagcgggct ggctgagagc agcaacaccg ggcaatgacg gggcgaatcg
      901 cgccagtcgg tcagtgcctc ggtaatctca atggtctcgg tcgctgatgc ttcggcgtcg
      961 tcctgattga gggtcgcctg gagcgcttcg agggcacgga gctggccggt gcgcgggagg
     1021 cggccggtgc cgcggacatg ctcgccgctt ggctgctccg agatcagatg atcttcgagt
     1081 gccgtgtggc tctcggcgcg gtaccagagc ctggtgcgtt ctcgccgtgg caggctgtcg
     1141 gtcgcagaag acaacgtcgt actcgcgtgc tgggacatga cgcgcatttc tccggtcttg
     1201 ttcctggtgg ttgccgggaa gccggcgttt cgataacgcc gcggggtgtg gaccactacc
     1261 tgattgtcat agcccgatag taatggcccg ggcggccatt cggagggacc cgcgggtcct
     1321 actccaaacg ggacgatggt accggtcggc tgaccggcga tactttggcc gtggatgagc
     1381 gagaccggac tgcgcaaccc cggcagcgaa cgatcagcaa actgccgttc gaccagcgcg
     1441 cgatcggcga gattgcggcg ttgctgggcg cgacgccgtc gctggcgccg ttccagttgc
     1501 caggtggcga ggtgcatcag ttgaccgtgc agcgcgatga agcgccgccg tcggtgctac
     1561 tgacgctgtg gccgacgatc cggcgggtgg atgcaatcag ccagtcggcg acggtcgtct
     1621 tcacgaacgt cgtgggcgtg gagctggtcg agggagtcga agtcctgttc cgccgcggta
     1681 ccggcgagta cctgatcgtg accgtggctg gcaaggtcat cgtccgggca taaccgggcc
     1741 ggcggagcac tacccgggcc tgaagacccg ggctgaaatc tgaacccggc tgaagccggc
     1801 tgaggatgcg cctgcgtggc cggcccgggc ccgggcccct ctaatgtttg ggttgggatc
     1861 gggcccgctg ccaggtcggc tcaatcgaac gagagcgggg atgcttgatg cggtccacaa
     1921 tggaccgcgc tacggtcgga gcgagtgcat gccaacgggc cggttgggac gcggcgggca
     1981 gatgccatca gcccctacat tgtgccattc ccaattcttc caaccaccat ccaccaacca
     2041 ccatccaccg ttccctcgac cctccaccct caacttaccg ttgcagggtg aggaagccgg
     2101 cgagggagat gccgaggacg atgacggcgg ctgcttgggc gatggtgttt tgccagccga
     2161 ccgatttgcc ccggaagagg cgcggcagga cccagacgcg gcgttgcgtg aatggcagac
     2221 cgggctggat gccggccctg gtgcaggcat cgccgagc
//
LOCUS       DASSGX010000318         2187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1364291, whole genome shotgun sequence.
ACCESSION   DASSGX010000318 DASSGX010000000
VERSION     DASSGX010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1364291"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1171
                     /locus_tag="VFI12_05615"
     CDS             <1..1171
                     /locus_tag="VFI12_05615"
                     /inference="COORDINATES: protein motif:HMM:NF017669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HET7055919.1"
                     /translation="PLLLARLLNLSDAQSGVLQLVFRYADDNGLLLLDLKDLRSLLQY
                     IGDNRKDVQTSYGNVSPQSVGAIQRSLLTLEEAGGDKIFGEPAISLSDFMRTDQHGRG
                     YINILASEQLINSPLVYATFLLWLLSEMFESMPEVGDPDKPKLVFFFDEAHLLFTECP
                     EALRDKIEQVIRLIRSKGVGVYFISQNPLDVPDIVLGQLGNRVQHALRAFTPRDQKAV
                     RSAAETFRPNPKFEVEQVITELGTGEALTSFLEEKGAPGVVERAFVVPPRSRIGVITP
                     EERAKVVNWSPVQGQYETPVDRESAYELLKVKAERAAQAAEAARTAQEQAKADEEFQK
                     QQDKLARSASRSRRSSSADATLETVARSAARSAGNSIGREIARGLLGSLFGGRRR"
     gene            1872..>2187
                     /locus_tag="VFI12_05620"
     CDS             1872..>2187
                     /locus_tag="VFI12_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055920.1"
                     /translation="MSEERAFRDLLDGVLTGKVTRREMMKRAAMLGLSASAVSTLSLA
                     AIAVPGTVSIVSAQDATPEPVAGGTLRMGMQADPSAFDPQKLSATAIWRVVEHMYNGL
                     TRI"
BASE COUNT          408 a          695 c          686 g          398 t
ORIGIN      
        1 cccactgctg ctggcacgcc ttctcaacct gagcgacgcc cagtccggtg ttctccagct
       61 cgtgttccgg tatgccgacg acaacgggct gctgctcctc gacctgaagg acctgcgctc
      121 gctgcttcaa tacatcggcg acaatcgtaa agacgttcag acatcctatg gcaacgtctc
      181 gccgcagtcc gtcggcgcca tccagcgctc cctgctgacg ttggaagagg ccggcggcga
      241 caagatcttc ggcgaaccgg cgatcagcct gtccgacttc atgcgaaccg atcagcacgg
      301 gcgcggctac atcaacatcc tggcctcgga gcagttgatc aactcgccgc tcgtctacgc
      361 gacattcctg ctctggttgt tgtcggaaat gttcgagtcc atgccggagg tcggggatcc
      421 cgataagccg aagctcgtct tcttcttcga tgaggcgcac ctgctcttca ccgaatgccc
      481 ggaggcgctc cgggacaaga tcgagcaggt aatccggctg atccgctcca aaggggtcgg
      541 cgtctacttc atcagccaga acccgctcga tgtgcccgac atcgtccttg gccagcttgg
      601 caaccgcgtt caacacgcgc tccgggcctt cacgccccgc gaccagaagg cggttcggtc
      661 ggccgccgag acgttccggc cgaacccaaa gttcgaggtc gagcaggtga tcaccgagct
      721 cggcaccggc gaggcgctga cctcgttcct cgaggagaag ggtgccccgg gcgtcgtcga
      781 gcgcgccttc gtcgtgccgc cgcgctcacg gatcggcgtg attactcctg aggagcgggc
      841 aaaggtcgtc aattggtcgc cggtgcaggg acagtacgag acaccagtcg accgggagtc
      901 ggcgtatgag ctgctgaagg tcaaggccga gcgcgccgcg caggcggctg aggcagccag
      961 aactgcccag gagcaggcga aggccgatga ggaatttcag aagcaacagg acaagctggc
     1021 gcgctccgcg agccggagtc gccggtcttc gtcggcggac gccacgctgg aaacagtcgc
     1081 ccggagcgct gcccggtcgg ccgggaactc aatcgggcgc gagattgcgc gggggcttct
     1141 gggtagcctg ttcgggggcc ggcggcgatg aaaccgggac taccggactg ttggtagccc
     1201 agccttcagt ggcacttgca ttcggcatgg cccgggtcta ccctgtaatc aggccactcg
     1261 ataagggccg cggaagacag agacaccatt cgataccgtg accaggtggc agggttgaat
     1321 ggaacggaaa cgcaccgcgc tgtagctcga gaatggccag aaagaggagg tgtgccatgc
     1381 acatgaatcg tatcctcttc ggggtatggc aagttgacag gtcacgcccc gattgtctcg
     1441 cggggcaagc gactccgggt aactcctgac tcacccgtgg gttgtgatgg ctccccagcc
     1501 aacccgtctt gtcgtcctct gacgatacgt gtgagataag ccaaccggct tcactcgaac
     1561 catcgagaca accagttcaa tagaccaaat gcacaccccg gcaacggttt tcgaaccgcc
     1621 ggggtgttgc cttgcccgcc gcatctcctc catccgtgtc cgattactga ttccctgatg
     1681 cgtttctgac cgccagccaa tagggccacg gtcggcgatt ggcggtccgt tcgtgctaat
     1741 ctcaccgcta cgctgatccg tcgcggatcg tcaactaccc tggttaccgt caaccaaccg
     1801 tgcgcgttgg actgccgcac ttcggcaggc gacggaacga gcccgggcca tggtgaaagg
     1861 atgttggaat cgtgagtgaa gagcgagcgt ttcgggatct gttagacggt gttctgaccg
     1921 gcaaggtcac gcgccgggag atgatgaagc gggcggcaat gctcggcctc tcggccagcg
     1981 ccgtatccac cctctcgctt gccgcgatcg cagtgccggg cacggtgtcg atcgtcagcg
     2041 cccaggacgc gacccccgag ccggtcgctg gcggcacgct ccggatgggc atgcaggccg
     2101 atccgtccgc tttcgacccg cagaagctct ctgcgaccgc gatttggcgc gttgtcgagc
     2161 acatgtacaa cggcttgacc cggatca
//
LOCUS       DASSGX010000319         2260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_929619, whole genome shotgun sequence.
ACCESSION   DASSGX010000319 DASSGX010000000
VERSION     DASSGX010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929619"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..1424
                     /locus_tag="VFI12_05625"
     CDS             270..1424
                     /locus_tag="VFI12_05625"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7055921.1"
                     /translation="MKVSTFTLERWMSRWETEVEFDIAESGVYPLTTRDVLNLLPEAA
                     RAGALDALLDLRLGYTEARGTRGLRETLATVYANTTADNILVTTGAIEANYLLFNTLL
                     DPGDHVVAVYPAYQQLYAVAEAIGCDVSRWELRPETDFQYDLDELRRLVRPDTKMIVV
                     NTPHNPTGAILSPEQLDEVYDLAGSVGAWLLCDEAYRWLDLPGQPPMAPPIRNKGGHG
                     ISVGTMSKPFGLPGLRIGWIAAPEEVVAACWSSRDYVSLSPARLSDALAMIAIQHRDA
                     IVIRNQAIAAENMAAATEWFAEHADLATWTPPKAGLLALMKYNLDVPSKDLSDRLAGE
                     YSVMLAPGSAFGFEGYLRIGIGQRPDLFREGLYRTAACFRDLKSTSRLPH"
     gene            1680..>2260
                     /locus_tag="VFI12_05630"
     CDS             1680..>2260
                     /locus_tag="VFI12_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014155166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7055922.1"
                     /translation="MAQAQYAPARAGELHPALMPVPGQEQGPIISVTGLSKRFGRHTA
                     LANLTFDVPRACIGLLGANGAGKTTLVRALLGLTNPTSGEAMVLGLNTRTHGIEIRKR
                     VGYMPESDCLPPGATAADFVAHMGEMSGLPAQAARQRAADVLYQVGLREERYRLIKGF
                     STGMKQRVKLAQALVHDPALVFLDEPTNGMDPQG"
BASE COUNT          383 a          777 c          728 g          372 t
ORIGIN      
        1 acttcggtga ttagtggata gtgaatagtg gctggttccg gcctatccac taatcactgg
       61 cgcctgacga ccgaaatctg acgtctgccg cctacaatac atagacccgg ccttgtcggc
      121 cgggcctatg cgggaagggg aggtggagcg tgtgcggcgc tccacttggg cttgggagaa
      181 cacatgcgac ggttggatcg ctgtgttctg tacctttctt acgcaacacg cgacggattg
      241 gtttcacaga gggggtgggg ttcgccgcaa tgaaggtgtc cacgtttacg ctcgagcgct
      301 ggatgtcgcg ctgggagacg gaagtcgagt tcgacatcgc cgaaagcggc gtctacccac
      361 tgaccacgcg ggacgtgctc aacctgctgc cagaagcggc ccgggccggc gcgctcgacg
      421 ccctgctcga tctccgcctc ggctataccg aagcccgcgg cacgaggggc cttcgcgaaa
      481 cactggcgac ggtctatgcg aatacgaccg ccgacaacat cctggtcacg accggcgcga
      541 tcgaggccaa ttacctcctc ttcaacactc tgctcgaccc gggcgaccac gtcgtcgccg
      601 tctaccccgc ctaccaacaa ctctatgcgg tggctgaggc gatcggctgc gacgtctccc
      661 gctgggagct gcggcccgag accgatttcc agtacgacct cgacgagctg cgacggctgg
      721 tccggcccga cacgaagatg atcgtggtca acacgccaca caacccgacc ggcgcgatcc
      781 tcagccccga gcagctggac gaggtctatg acctggccgg ctctgtcggc gcatggttgc
      841 tctgcgacga ggcgtatcgt tggctggatc tgcccgggca gccgccgatg gccccgccga
      901 tccggaacaa gggcggccac ggcatcagcg tcggcacgat gtcgaagccg ttcggcctgc
      961 cgggcctgcg gatcggctgg atcgccgcgc cggaagaggt cgtcgcggcc tgctggtcct
     1021 cacgcgatta cgtctcgctc tccccggccc gcctcagcga tgcgctggcg atgatcgcga
     1081 tccagcaccg ggacgcgatc gtgatccgca accaggcgat cgccgccgag aacatggcgg
     1141 ccgcgaccga gtggttcgcc gagcacgccg atctcgccac ctggacgccg ccgaaagccg
     1201 ggttgctcgc gctgatgaag tacaacctcg acgtgccgtc gaaggatctc tccgaccggc
     1261 tggccggcga gtacagcgtc atgctcgcgc ccggctccgc cttcggcttc gagggctacc
     1321 tgcggatcgg gatcgggcag cggccggacc tcttccgcga agggctatac cggacggccg
     1381 cctgtttccg ggacctgaaa tcgaccagtc gccttccaca ctgatggtag actcagccag
     1441 atggagccca cgctcggtct ccatgcacac gttgtgcgga caagacgacc tggaacgtag
     1501 cgcggggcgg ctcgccccgc atcgcccgta gggcgcttcc cgttgctgac cggcgcgttt
     1561 cccagtgcga atatccgcct ttggcggagg cggggcgagc cgccccgtgc tactacatca
     1621 gctggcattg gtgaagcggc ccgcatgtac caggatcgcg gtagaggcag gtttcacgcg
     1681 tggcacaagc ccaatatgct ccagcgcggg caggtgaact ccatcctgcg ctgatgccgg
     1741 tgcccggcca ggaacagggg ccgatcatct cggtgaccgg cctgtccaag cggttcggca
     1801 ggcacactgc cctcgccaat ctcaccttcg atgtcccccg cgcctgcatc ggattgctcg
     1861 gcgcgaacgg agccggaaag accacgctcg tccgggcgct gctcgggctc accaacccga
     1921 cctccggcga agcgatggtg ctcggtctca acacccgcac gcacggcatc gagatccgca
     1981 agcgggtcgg ctacatgccc gagtccgact gcctgccgcc cggggcgacc gccgccgatt
     2041 tcgtcgcgca catgggcgag atgagcggct tgcccgcgca ggccgcccgc cagcgcgccg
     2101 ccgacgtcct ctaccaggtc gggctgcgcg aggagcgcta ccgcctgatc aagggcttct
     2161 cgaccggcat gaaacagcgg gtcaagctgg cccaggcact ggtccacgac ccggcgctgg
     2221 tcttcctcga cgagccgacc aatggcatgg acccgcaggg
//
LOCUS       DASSGX010000320         2871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1253820, whole genome shotgun sequence.
ACCESSION   DASSGX010000320 DASSGX010000000
VERSION     DASSGX010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253820"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VFI12_05635"
     CDS             <1..702
                     /locus_tag="VFI12_05635"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET7055923.1"
                     /translation="VLGDNQYEMGKLLKFQESYGPTWGRFKDITYPIPGNHEYQTTNA
                     AGYFAYFGAVAGNPTKGYYSFDIGTWHIVALNSECVQIGGCKAGSPEEQWLKADLASH
                     PAMCTLAMWHRPEFDSGDYASTSFLAFWTDLYNAGADVILNGHSHIYERFAPQTPNGT
                     LDTAYGIRQFTVGTGGDDHTPINKIKPNSETRNATTFGVLKLTLHASGYNWQFIPIAG
                     ETYTDSGSADCHGAP"
     gene            842..1741
                     /locus_tag="VFI12_05640"
     CDS             842..1741
                     /locus_tag="VFI12_05640"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET7055924.1"
                     /translation="MPPATITSQATTAPSATSIVPAAATATRVSTTKRPLLDHIYLIM
                     MENKESTSIVGSSNAPYLNSLIQQYGVAKDYTGVAHPSQPNYLALWAGSTFGITDDSI
                     HNLTGETLGDQIEAANMTWRVYAENYPAGASCYTDSSANGGPDGAGSYARKHNPAMSF
                     TNVNADATRCSQHITNFSHFDAAAAAFSLIVPNLCHDMHDCPVKQGDAWLQQWLPQHI
                     LNTSTWKNSNSAIFITWDEGTSATGGGGTVPLIVISHKTPPGFVSTKPYNHYSLLRTI
                     ENAWGFYCLRDACQAPDLNEFFP"
     gene            complement(1907..>2871)
                     /locus_tag="VFI12_05645"
     CDS             complement(1907..>2871)
                     /locus_tag="VFI12_05645"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET7055925.1"
                     /translation="LVHHRRRLRRGRHDAGHRLMRADKERYRALLRSVRPDLSTARFD
                     DLGEGWDNILFVIDEERIVRFAKDERTSRMLESEASLLQAIGPALPVRTPHPEFVARS
                     PVAPDIALMIYRRIPGLSLDDIALDDALVAGIAADLARFLDVLHGISPGLLDAIEIPR
                     FTPDDWVERHQMLYRRTRDAVRRGLGTMEFERYEAWWSDYLTNPEYRAFEPRLIHGDL
                     APEHVLIEREPWRLSGIIDFGDAMWADPALDLAGLPDLLARAVVTAMQSITPDWVFWA
                     RRAAYRRIAPLHAVFAGRYLGRPELLRGGIDALRRIFGQLETSL"
BASE COUNT          607 a          964 c          806 g          494 t
ORIGIN      
        1 gtgctcgggg acaatcagta cgagatggga aaactgctga aatttcagga gtcgtacggc
       61 ccaacctggg ggcgattcaa agacatcacc taccccatcc ccggcaacca cgagtatcag
      121 acgacgaatg ctgccggata cttcgcctat ttcggagccg tagcgggtaa cccgacgaag
      181 ggctactact ccttcgacat cggaacctgg cacatcgtgg cgctcaacag tgaatgcgtt
      241 cagatcggcg gctgcaaagc agggagcccg gaggagcaat ggctcaaggc cgacctggcc
      301 agccatcccg cgatgtgcac cctggcgatg tggcatcggc cagagttcga ctcgggagat
      361 tacgcctcaa cctcgtttct cgccttctgg accgatctgt ataacgcggg tgcggatgtt
      421 atcctcaacg gccactcgca tatctatgag cgcttcgcgc cgcaaacacc caacggcacg
      481 ctcgacacag cctacggcat acggcagttc accgtcggga ctggtggtga cgaccatact
      541 ccgatcaaca aaatcaagcc gaacagcgag acacggaacg cgaccacctt cggcgtactg
      601 aagctgacgc ttcacgcttc cggctacaac tggcagttca tcccgatcgc gggagagaca
      661 tataccgatt caggaagtgc ggattgtcat ggcgcaccat aacactggtc acttgactca
      721 cagagtgcgg cgactgcccg gcgcggccgc cgccctcctc gtagcgttgc tcatcctcgc
      781 cggctgcggg acagccgcga tcgctccaac gcgggtggcg acgagctcac cgcccccgac
      841 aatgccgccc gccacgatca catcgcaggc cacaaccgcg ccgagtgcaa ccagcatcgt
      901 tccggctgcg gctacagcca ctcgcgtgtc gacgacgaag cggccgctgc tcgaccatat
      961 ctatctcatc atgatggaga ataaagagtc gacgtcaatc gttggcagca gcaatgcgcc
     1021 gtatctcaat tcgctcatcc aacagtacgg cgtggcgaaa gactacaccg gagtcgctca
     1081 tccgtcgcaa ccaaactacc tcgcgctctg ggccggttcg acgttcggga tcaccgacga
     1141 cagtatccac aacctgaccg gtgaaacgct cggagatcag atcgaagcgg caaacatgac
     1201 ctggcgtgtc tacgccgaaa attacccggc cggtgcgtcc tgctacacgg attcttccgc
     1261 caacggcggt cccgacggtg cgggctcgta cgcgcgcaaa cacaacccgg ccatgagctt
     1321 caccaacgtg aatgccgatg ccacccgttg cagccagcac atcaccaatt tcagccattt
     1381 cgacgcggcc gctgctgcct tcagcctcat cgttccgaac ctctgccacg atatgcacga
     1441 ctgcccggtg aaacagggcg atgcctggct gcagcagtgg ctgccgcagc acatcctcaa
     1501 cacgtcaacc tggaaaaact caaatagcgc gatctttatc acctgggatg aaggcacgtc
     1561 cgctacgggc ggcggtggca cggtcccgtt gatcgtcatc tcgcacaaga cgccgcccgg
     1621 cttcgtctcg accaagccgt acaaccacta ctcgctcctg cgtacgatcg aaaacgcctg
     1681 gggcttctac tgcctgcgcg atgcctgcca ggcccccgac ctcaacgagt tcttcccatg
     1741 atgcgcctcc ggtgtagggg tttgattgat taaacccggc gcgccgttca ggtggcgctc
     1801 gtcttcccca ttccccaggc atccgcgttg ccaatcctcg acacgctcgc caaccacacg
     1861 cctcgtgcgc tgcgcgcaca tcggctgggt ttgatcaatc aaacccctac agggatgttt
     1921 ccagttggcc gaagatgcgc ctgagcgcgt cgatcccgcc ccgtagcagt tcaggcctgc
     1981 cgagataccg accggcgaaa acggcatgca acggcgcgat ccgacgatac gcagcacgcc
     2041 gcgcccagaa tacccaatcg ggcgtgatgg actgcatcgc cgtgacgacc gcccgtgcga
     2101 gcaaatcggg cagccccgcc agatcgagcg ccggatcggc ccacatggcg tcgccgaagt
     2161 cgatgatgcc gctcaggcgc cacggttctc gttcgatcag cacatgctcc ggcgccagat
     2221 cgccatggat cagacgcggc tcgaacgccc ggtactccgg attcgtcaga tagtcgctcc
     2281 accatgcctc gtatcgctcg aactccatgg tacccagtcc ccgccgcaca gcgtcgcgtg
     2341 ttcgacggta gagcatctga tggcgctcaa cccagtcgtc cggggtaaac cgtggaatct
     2401 cgattgcatc gagcagaccc ggtgagattc cgtgcagcac gtcgagaaag cgcgccaggt
     2461 ctgccgcgat cccggcgacc agcgcatcgt cgagcgcgat gtcgtcaagt gacaggccgg
     2521 gaatccgccg gtagatcatg agcgcgatat ccggcgctac gggagatcgc gcgacgaatt
     2581 cggggtgtgg cgtccgcacc ggcagcgccg gaccgatcgc ctgcaagagc gacgcttcgg
     2641 actccaacat gcgcgacgtc cgctcatcct tcgcgaagcg cacgatccgc tcctcgtcga
     2701 tgacgaagag gatgttatcc caaccctcgc cgaggtcgtc gaaacgcgcg gtcgataggt
     2761 caggccgaac cgagcggagt aacgcgcgat agcgttcttt atctgccctc atcaagcggt
     2821 gaccagcgtc atgacgcccg cgacgaaggc gacgacgatg atgaaccagc g
//
LOCUS       DASSGX010000321         2315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_930020, whole genome shotgun sequence.
ACCESSION   DASSGX010000321 DASSGX010000000
VERSION     DASSGX010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2315
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930020"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="VFI12_05650"
     CDS             <1..642
                     /locus_tag="VFI12_05650"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET7055926.1"
                     /translation="MAYFYLLEKPFLRQTLLENIQPGSRLRVAATWDAVEESIGGWLR
                     SRIILGVIVGIITIIAFGALRLPYWPLLGLLAGLTEPIPILGPWIGGIPAVFLALTVS
                     PVKAVIVALFILGRQMLVDAVLVPRVTRDAVGLSPLTVFAAVLAGTELIGPAGAFLAI
                     PLAAAVQILIVDALAARRQITEPRPALRWRWLLNPEESVPDVAGPGDRPTRGG"
     gene            complement(794..2176)
                     /locus_tag="VFI12_05655"
     CDS             complement(794..2176)
                     /locus_tag="VFI12_05655"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET7055927.1"
                     /translation="MTNYRDVSYWLESAGDDLTPRPALPESIDVDVAILGAGYSGLWT
                     AYYLKERDPSLEIAIIEREIAGFGASGRNGAWCTSEFPTGPGALAHTYGDDTARAVVT
                     AMRETVTEIKRVTKAEGIACDWADGGQLLIARGPHQRPAIDDEYQTLSRLRLADGLEL
                     LDADQARARVNVTELTGGLFSPYTAVIHPGKLVRGLARAVEGRGVRIYEQTAVTRVHP
                     GRYPTLETDRGNVRAKAIVLCGEAYLSQVPGYGRAVMPVYSLITLTEPLSEADWAEIG
                     WRNRECVASCRYTVDYLSRTADGRILFGGRGAPYRFGSKITPEMDKHAGTHQLLQDNV
                     RAWFPRLKDVRFTHTWGGPLGWSRDFMPTVSFDKRQGLGMAHGYTGNGVATTNLAGRT
                     LAAMITGQQSDLLHLPHANHTTRPWEPEPFRYLGVRFVQQSFMRIDRQAEATGIAPSG
                     TSLAERLTAH"
BASE COUNT          353 a          705 c          857 g          400 t
ORIGIN      
        1 atggcctact tctacctgct ggaaaagccg ttcctgcggc agacgctgct ggagaatatc
       61 caacccggga gccggctacg ggtcgcggcg acctgggacg cagtcgagga gtcgatcggc
      121 ggctggctgc ggagccggat catcctcggc gtgatcgtcg gcatcatcac gatcatcgcg
      181 ttcggcgcgc tgcggctgcc ctattggccg ctactggggt tgctggccgg gttgactgaa
      241 ccgattccga ttctcggacc gtggatcggc gggatcccgg cggtgttcct cgcgctgacc
      301 gtctcgccgg tcaaggcggt catcgtcgcg ctgttcatcc tggggcggca gatgctggtc
      361 gacgcggtgc tcgtaccccg ggtgacccgg gacgccgtcg ggttgtcgcc gctgaccgtc
      421 ttcgcggcgg tgctggccgg gacggagctg atcgggccag cgggggcgtt tctggcgatc
      481 ccgctggcgg cggcggtgca gatcctgatc gtggacgcgc tcgcggcgcg ccggcagatc
      541 acagaacccc ggccggcact gcgctggcgg tggctgctca atccggagga gtcagtcccg
      601 gatgttgccg gtcccggaga tagaccgacg cgcgggggtt gaatacgcca ccggcccggt
      661 gccacaccgg aatgaacctg cgccgggggt tgaaaccccc gtctaagaga cggaagtcgg
      721 ttgaaaccaa ctaccagtgt tcgataccca ggccagcccg ggaacaccca tcattcctga
      781 actcccaacc tggttaatgg gcggtgaggc gttcggcgag ggacgtacca gagggggcga
      841 tgccggttgc ttcggcttgg cggtcgatgc gcatgaagga ttgctggacg aagcgaacgc
      901 cgaggtagcg gaatggttcc ggttcccacg ggcgggtcgt gtgattcgca tgcggcaggt
      961 ggagcaggtc ggattgctgg ccggtaatca tggcggcgag ggtgcgaccg gccagattgg
     1021 tcgttgcgac gccgttgccg gtgtagccgt gggccatgcc gagaccctgc ctcttatcga
     1081 aggagacagt cggcatgaaa tcgcgcgacc agccgagcgg gccgccccag gtgtgggtga
     1141 agcggacgtc tttcaggcgc gggaaccagg ctcggacgtt gtcttgcagc aactggtgcg
     1201 tgccggcgtg cttgtccatc tcgggcgtga tcttcgagcc gaagcggtag ggcgcgcccc
     1261 ggccaccgaa caggatgcga ccgtcggcag tgcgcgacag gtaatcgacc gtgtaccggc
     1321 aggaggcgac acattcgcgg ttgcgccagc cgatctcggc ccaatcggct tcggagagtg
     1381 gttcggtcag ggtgatcagc gagtagaccg gcatgacggc gcggccgtag ccgggcacct
     1441 ggctgaggta ggcctcgccg cagaggacga tcgccttggc gcggacgttg ccgcgatcgg
     1501 tctcgagcgt cgggtagcgg ccgggatgga cgcgggtgac agcggtctgc tcatagatac
     1561 ggacgccacg cccttcgacg gcccgggcga ggccgcggac gagcttgccg gggtggatga
     1621 cggcggtgta gggcgagaag agcccgccgg tcagctcggt cacgttgacc cgcgcccgcg
     1681 cctgatcggc gtccagcagc tccagcccgt cagcgaggcg aaggcggctc agggtctggt
     1741 actcgtcgtc gatcgccggg cgctgatgcg ggccacgggc gatcagcagc tgaccgccat
     1801 cggcccagtc gcaggcgatc ccttcggcct tggtgacgcg cttgatctcg gtcacggtct
     1861 cccgcatcgc ggtgacgacg gcgcgggcgg tgtcatcgcc gtatgtgtgg gcgagggcgc
     1921 cgggaccggt cggaaactcg ctggtgcacc aggcgccatt gcggccgctg gcgccgaaac
     1981 cggcgatctc ccgctcgatg atcgcgatct cgagcgacgg gtcgcgctcc ttgaggtagt
     2041 acgcagtcca gagcccggag tagccggcgc ccaggatcgc gacgtcgaca tcaatcgatt
     2101 cgggcaatgc cggccggggc gtgaggtcgt cgcccgcgga ttcgagccag tacgagacgt
     2161 cgcggtaatt ggtcatttcc ctaccctgca aggttgatgg cggcgttgag gagcaggttg
     2221 gcgccggctt cgatgtcggc cggcgtgctg tattcggttt cgcagtggct gagtccgcct
     2281 tcgctgcgga cgaagatcat cccgctcggg cagac
//
LOCUS       DASSGX010000322         2869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_710222, whole genome shotgun sequence.
ACCESSION   DASSGX010000322 DASSGX010000000
VERSION     DASSGX010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710222"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1200)
                     /locus_tag="VFI12_05660"
     CDS             complement(<1..1200)
                     /locus_tag="VFI12_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055928.1"
                     /translation="MSNQELDPITDTRVPEAPKTDAEPLFPGAGMDDELDPMPDALSP
                     DAASAGLDDIVLPQPVAGRPVVSPDGATVAIIQPDQTGRDRIWLAPVDGGEAEILDVD
                     LELASDANPNGPQWSPDGTRLAVTAPHPADGRSAIWEIVVATKLARVLVEHEAADSSP
                     IWSPDGEWIAFLSRRFGRTAASVIRADGLGDPLQVSHAPRGFDDHSLTWSRDSARLAF
                     AREAVDGDKVGDHIHTVEVKTGGTKQVTTRLAGRHSLTWAPDRNLIMHVANDSEWDHV
                     AVVNADNASGWNIAAEKGDKSDPRWSPDGQRVVYLRRLDGVVRCCERGTSTATSESID
                     PGEGVASGAQFLSDKRVCYVYQSATTGPQVIVQEPQPDAERTVLPIIPEWTPDRSLTT
                     PHYQSFEI"
     gene            1471..>2869
                     /locus_tag="VFI12_05665"
     CDS             1471..>2869
                     /locus_tag="VFI12_05665"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HET7055929.1"
                     /translation="MSRVSGSLVRISAVVALLMSFALGMLAHPAAVGAQKTSKNETGV
                     SGSTYTSPSFGYSIEWDRSWDVTDETVEDDYNMLYLNDGTSLLYFEGLAPASAVDECV
                     STYVATYENLKGVSEFESTKPEESADGVLSTEFSFVLTYTDEDTGDDVAIDTQGYVSC
                     QEINDGDANLAITHIAPADNWDDEVPAREDVVSTLTFDGETPSTNTPDKEETPETTPD
                     EPSNNDSGTGQMTDVQGDGELPANADELLSLFQTSVSDINDYWAREYPLISGGQTYEP
                     PAAFVPWTGTIDTACGPADSFDANAGTGMGPFYCPPDQTIYLDMGFANFQFDAVGDVP
                     FLIPVVLAHEVGHHVQDELGMQVCYQTPCLDPNVLTSQEIEYMADCYAGAWSRDAELR
                     GRLGSSDIDANIVQYAVLLGGGKEGADPGGHGEGPKRVWWFLNGYVEGAAKCFETSKV
                     TAGWAQSGPPNANASA"
BASE COUNT          503 a          937 c          950 g          479 t
ORIGIN      
        1 gatctcgaag ctctggtagt gcggcgtggt cagcgaccgg tccggcgtcc actcggggat
       61 gatcggaagc acagttcgct cggcatccgg ctgcggctcc tggacgatca cctgcggacc
      121 agttgtcgcg gactggtaga cataacacac tcgcttgtca gacagaaact gcgcaccgga
      181 ggcgacgcct tcgcccggat cgatcgactc cgaggtcgcg gtgctcgtcc cgcgctcgca
      241 acagcggacg acgccatcaa gccggcgcag gtaaacgacc cgctgtccgt ccggcgacca
      301 gcgagggtcg gacttgtcac ccttctcggc ggcgatgttc cagccggagg cgttgtcggc
      361 attgacgacg gcgacgtggt cccattcgct gtcgttggcg acgtgcatga tcaggttgcg
      421 gtccggcgcc cacgtcaacg aatggcgtcc ggcgaggcga gtggtgacct gcttggtccc
      481 gcccgtcttc acctcgacgg tgtggatgtg atcgccgacc ttgtcgccgt cgaccgcttc
      541 ccgcgcgaag gcgagccggg cgctgtcccg cgaccaggtg agcgagtgat cgtcgaagcc
      601 gcgcggcgcg tggctgacct gcagcggatc gccgagcccg tcggcccgga tcacgctcgc
      661 ggcggtccgg ccgaagcggc gggagagaaa ggcgatccac tcgccatccg gcgaccagat
      721 cggggacgaa tcggcagcct cgtgctcgac caggactcgc gccaacttcg tggcgacgac
      781 gatctcccag atcgcggagc gaccatcggc cgggtgcggc gcggtcacgg ccagacgcgt
      841 gccatctggc gaccactgcg ggccgttcgg gttagcgtcg ctggccagct ccaggtcgac
      901 gtcgaggatc tcggcctcgc caccgtcgac cggcgccagc cagatccggt cccggccggt
      961 ctgatcgggc tggatgattg cgacggtagc gccatcgggc gagacgacgg gccggccggc
     1021 aaccggctgc ggcagaacga tgtcgtcgag gccggcactc gccgcgtcgg gcgagagggc
     1081 gtccggcatc ggatcgagct cgtcgtccat accggcgccg ggaaagagcg gctcggcatc
     1141 agtcttgggc gcctcgggca cgcgtgtgtc cgtgatcgga tcgagttcct ggttgctcat
     1201 agtggcttgg ttcaccgcac ggtaaggctg attcgtaggc gacaactata gtagacagcc
     1261 cgtccagtaa tgtctgccgg gaaccagtcc tattctcccg gcgttgaata tccggtactg
     1321 gatgcgagtt ggccgccgtt gccgtcttga cacggcgata cggtcatgta cgctgactaa
     1381 gcggtccgat atagggtggg ttgtaccgcc gtggaaaccg gcggtcattt tgtgtggctt
     1441 gggcggagaa atcgccccgg ggagatctgc atgagtcgcg tatctggctc tcttgtgcgc
     1501 atcagcgccg tcgtcgcgct gctgatgtcg ttcgcgctcg gtatgctggc gcatccggcg
     1561 gccgtcggcg cgcagaagac gtcgaagaac gaaaccggcg tatccggcag cacctacacc
     1621 agcccgtcct tcggctacag catcgagtgg gatcgctcgt gggacgtcac ggatgagacg
     1681 gtcgaagacg actacaacat gctgtacctg aacgacggca ccagcctcct ctatttcgag
     1741 gggctcgctc cggcgtccgc cgtcgacgag tgcgtgagca cctatgtcgc cacgtacgag
     1801 aacctgaaag gtgtgagcga attcgagagc acgaagccgg aggaatcggc cgatggcgtg
     1861 ctcagcaccg agttcagctt cgtcctgacc tataccgatg aagacaccgg cgacgatgtc
     1921 gcgatcgaca cgcaggggta cgtctcctgc caggagatca acgacggcga cgcgaacctg
     1981 gccattaccc acatcgcccc cgcggacaac tgggatgacg aggtcccggc ccgagaagat
     2041 gtcgtcagca cgctgacctt cgacggcgaa accccatcca ccaacacccc ggacaaggaa
     2101 gagacgcccg aaaccacgcc ggacgagccg agcaacaacg actccggcac gggccagatg
     2161 accgatgtcc agggcgacgg cgagctgccg gccaacgccg atgagctgct gtccctgttc
     2221 cagacctcgg tctccgatat caatgactac tgggcgcgtg agtacccgct catctccggc
     2281 ggtcagacgt acgagccgcc ggcggcgttc gttccgtgga ctggcaccat cgacacggcc
     2341 tgcggtcccg ccgattcctt cgatgcgaac gccggcaccg gcatgggccc gttctactgc
     2401 ccgccggacc agacgattta cctcgacatg ggcttcgcca acttccagtt cgatgcggtc
     2461 ggcgacgttc cattcctgat cccggttgtg ctcgcccatg aggtcggcca ccacgtccag
     2521 gacgagctcg gcatgcaagt ctgctaccag actccgtgcc tcgacccgaa cgtgctgacg
     2581 tcgcaggaaa ttgagtacat ggccgactgc tatgccggcg cctggtcccg cgacgcggag
     2641 ctgcgcggcc gcctcggctc gagcgacatc gacgccaaca tcgtccagta cgccgtcctc
     2701 ctcggtggcg gcaaggaagg cgccgatccg ggcgggcacg gtgaagggcc gaagcgcgtc
     2761 tggtggttcc tgaacggcta cgtcgagggc gcggccaagt gcttcgaaac aagcaaggtg
     2821 accgccggct gggcgcagtc cggtccaccg aacgccaacg cgagtgccg
//
LOCUS       DASSGX010000323         7356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1474402, whole genome shotgun sequence.
ACCESSION   DASSGX010000323 DASSGX010000000
VERSION     DASSGX010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7356
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1474402"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1296
                     /locus_tag="VFI12_05670"
     CDS             <1..1296
                     /locus_tag="VFI12_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7055930.1"
                     /translation="TAGQYQSILFEIKGCEEFASSLTPGEGTPEADPAAYETTKAALG
                     AKVIDDTTLELTLTHPAPYYPTVASLWVFYPAKKELVEQGGDDWWKKPELQIGNGPFQ
                     MTQFEDNQLIGFKANENYWGGRPKLDGVEIVYQGEYAVALEAYRAGDLDIVQVDPQQI
                     PEIQADPELSQQIVQYPVASTYTLNFNLTMEPFNDIKVRQAFSQAFDRETYCAVVRNG
                     DCSPTLSWIPAGLPGSIETDKYGFDAEAAKAALAESSYGSADKLPPIKLYYNSNDPAN
                     TARAEWVAGQYRDILGVTITLEPTEGTALVALRKEPTTFPQMLLVGGWIQDYPDPQNW
                     LSVYFKCDATFAKRFAYCNPEFDKLVDQADVEFDQTKRMELYQQAGQILVDDIPGPFL
                     YNLSNIVLVKPNVTGYTATAIDAEWPGQFGSLLTVDKSE"
     gene            1483..2496
                     /locus_tag="VFI12_05675"
     CDS             1483..2496
                     /locus_tag="VFI12_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7055931.1"
                     /translation="MLNFVIRRLLWIVPVIVVVSAATFFLMYQAPGGPWDLEKPMPPQ
                     AVANLNKKFGLDKPQWINYDKFSTELDNGERNPVSLAGAFLDSQFFNYLGGVFTGDLG
                     PTYASRGSETVQHVIVSQFPVSARIGLVAILFAICVGVPLGVIAALRQNTWADYLSLL
                     TSTIGISVPTFVSGLILIIFMSRQFHVSPIKRPEAWQGLFSTAYILPGIVLGLGTTAY
                     ITRLTRSSMLEVMKNDFVRTARAKGLSGGVVVRRHMLRNALIPVITIIGPATADLVTG
                     SIIIETIFGAPGIGKEFVTSIGKRDYSVIMGITIFYAVLIAIANVLVDLSYGFIDPRI
                     RRR"
     gene            2574..3536
                     /locus_tag="VFI12_05680"
     CDS             2574..3536
                     /locus_tag="VFI12_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7055932.1"
                     /translation="MDDSPLTAMRPQRSLWSNAWRRLRKNLMAMAALCYLFFLLMVAI
                     LAPVIAPHNPVKTDVAHAGTLRKAAWVEDPNPLRTGTWDYPLGTDAVGRDVFSRLVYG
                     TRVSLVVGFIPMAVILLIGVPVGLIAGFAGGRVDNLLMRMTDVVYAFPALLLAIIMQI
                     SFGDTSIGKLLNGLVLLFISLSIVNWTGVARLVRGETLSLKEKEFVEAARTSGASRFR
                     LITRHILPNALGPIIVAGAFIVPGAIISEAILSYLGVGVRPDVNLNAPFPTSWGQMIL
                     DGSKTYNSQLWMLLAPSIAVALTTLSFTFVGDGLRDALDPRQSD"
     gene            3543..4286
                     /locus_tag="VFI12_05685"
     CDS             3543..4286
                     /locus_tag="VFI12_05685"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7055933.1"
                     /translation="MRERQDGPTYSDADVAALYDVLNRWGPGDDFYLTLIRDAESVLD
                     VGCGTGTLLHHARQAEHTGRLCGVDPDQAILAVARSHRGIEWCDGTAASMTWTGEFDL
                     AVMTGHAFQCLITDEEVRASLVAIRHALVPGGRFAFETRNPLVRAWEHWDGMDPIEAV
                     DPQGRAVRVSYDVEDIVEDVVALAEITCDSENRRLRVDRARLRFMDNQLLTDFLDGAG
                     FDIEAQYGNWDRSALSPSSPEIITIARTR"
     gene            4417..6714
                     /locus_tag="VFI12_05690"
     CDS             4417..6714
                     /locus_tag="VFI12_05690"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HET7055934.1"
                     /translation="MPRTSKATLISEQSASDGIPIPTALRPPATFPADRLIEVARSRL
                     QSELVRRNDGAVPAAIERAVDRAIEWHAGQVRKSGEPYVVHPIEVAILLSRMSVDSEA
                     IIAALLHDVVEDSSVGLSTIREEFGDRVALLVDGVTKLGQIQWTGDGDQATRERAVQA
                     ENLRKMFLAMIDDIGVVLIKLADRLHNMRTLDHMSRDKQIRIAQQTMEIYAPLANRLG
                     IWQFKSELEDLAFRYLQPEAYQSIKARLEERGRDQAAYIAQVMQDLKDAIQAEGIDVE
                     LTGRTKHTYSVYRKMKQKQRTFDEIYDVIGIRCIIPDDRIKDCYGVLGVIHSMWHPIP
                     GEFDDYIATPKQSMYQSLHTAVIGPQGHALEIQIRTQRMHEIAEFGVAAHWRYKEGKK
                     ANAKVEAKVAWLRQMLEWQAEMKDAEDFVETVKSDVLEEMIYVFTPRGDIIELPAGAT
                     PLDFAYRIHTEVGHHTVRAKVNDQLVRLDTKLKNGQVVEISTSKTKLGPSRDWLREDL
                     GYLTTASAKEKIRQWFRRQEREENLAQGKEILEKELRRLGVDLKPEDIYKHFPRYSKV
                     EDMIAAIGYGAISPRLIAARLGETETKNVLSDTPHVPKPRVPLRLDVMGVGDLLTSLA
                     TCCKPVNGDSIVGYVTRGRGITVHRADCSNLVNLPDPERLIQVTWGSKAGETYPVNIM
                     VRAWDRVGLLKDISTLLADEKVNILYVQTVTNDDRTVNLRITIEVENVSQLSRVLHKI
                     ESVPAVDEVRRDTNNGATDTRLAGD"
     gene            6719..>7356
                     /locus_tag="VFI12_05695"
     CDS             6719..>7356
                     /locus_tag="VFI12_05695"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET7055935.1"
                     /translation="MTDSRTVLCLGEVLIDMIVSDGSPTLEDAGEFVARPGGAPANVA
                     VALRRLGVPSAFCGAVGDDPFGARLRRVLIADGVDTAGLMIVPGQATTLAFAWKDERG
                     DGHFRLVRLADAALSALAVENAGIPERAAIIVGSVALTEEDSRTAIYRAVEIAGDSGV
                     PVCFDVNMRPTLWRSVEDARAACQPVIDMVTMLKLSVDDAEILFGRREPEAI"
BASE COUNT         1358 a         2349 c         2294 g         1355 t
ORIGIN      
        1 acggccggcc agtaccagtc gatcctgttc gagatcaagg gctgcgagga gtttgccagc
       61 tcgctgacgc ctggtgaagg cacgcctgag gctgatccgg ccgcgtacga gacgaccaag
      121 gccgctctcg gcgccaaggt gatcgacgac accacgctgg agctcacgct gacgcacccg
      181 gcgccctact atccgacggt tgcctccctc tgggtcttct acccggccaa gaaggagctc
      241 gttgagcagg gcggcgacga ttggtggaag aagccggagc tgcaaatcgg caacggcccc
      301 ttccagatga cccagttcga ggacaaccag ctcatcggct tcaaggccaa tgagaactac
      361 tggggcgggc ggccgaagct cgatggcgtc gaaatcgtct accaaggcga gtacgcggtc
      421 gcgcttgagg cgtatcgcgc cggcgacctc gacattgtcc aggtcgaccc gcagcagatc
      481 cccgagatcc aggccgatcc ggagctgagc cagcagatcg ttcagtaccc ggtcgcctcg
      541 acctacacgc tcaacttcaa cctgaccatg gagccgttca acgacatcaa ggtccggcag
      601 gcgttctcgc aggcattcga ccgcgagacc tactgcgccg tcgttcgcaa tggcgactgc
      661 agcccgaccc tgagctggat cccggccggg ctcccgggct cgatcgagac tgacaagtac
      721 ggcttcgatg cggaggctgc caaggccgcc ctcgccgagt cgagctatgg cagcgcggac
      781 aagcttccgc cgatcaagct ctactacaac agcaacgacc cggcgaacac cgcccgcgcc
      841 gagtgggtgg ccggtcagta ccgcgacatc cttggtgtga ccatcacgct ggagccgacc
      901 gagggcaccg ctctcgtcgc cctgcgcaag gagccgacca ccttcccgca gatgctgctg
      961 gtcggtggct ggatccagga ctacccggat ccgcagaact ggctgagcgt ctacttcaag
     1021 tgcgacgcca ccttcgcgaa gcggttcgcc tactgcaacc ctgagttcga caagcttgtc
     1081 gaccaggcgg atgtcgagtt cgatcagacc aagcgcatgg agctctacca gcaggctggt
     1141 cagatcctgg tcgatgacat tccaggcccg ttcctctaca acctctcgaa catcgtcctc
     1201 gtcaagccga atgtgaccgg ctacacggcg acggcgatcg atgcggagtg gccgggccag
     1261 ttcggctcgc tgctcactgt cgacaagtcc gagtaggacc cgacaacccg ggggttggcg
     1321 catgcttgcg ccaacccctc ttcacgcgtc cagtctcgtt tcgtttccgc tctgtttttt
     1381 agaccgcttg ttcgttctgg cccatgcgtc tcgccggcag tattatggag acctggcatg
     1441 gctccgtgat acacagcccc cgatgagcgg ttcgaggtct ccatgctgaa tttcgtcata
     1501 cgcaggctgc tctggattgt gcctgtgatc gtcgtcgttt cggcggcgac cttcttcctg
     1561 atgtatcagg cgccaggtgg tccgtgggac ctcgaaaagc cgatgcctcc acaggcggtc
     1621 gccaacctga acaagaagtt cggattggac aagccccagt ggatcaatta cgacaaattc
     1681 agcaccgagc tcgataacgg cgagcggaat ccggtctcgc tggccggcgc attcctcgac
     1741 agccaattct tcaactacct cggtggcgtc ttcaccggcg acctgggtcc gacctacgcg
     1801 tcgcggggca gtgagacggt gcagcacgtc atcgtcagcc agttcccggt ctcagcgcgg
     1861 atcggcctgg tggcgattct cttcgccatc tgcgtcggcg tgccgctcgg cgtcattgcc
     1921 gcgctccggc agaacacctg ggccgactat ctgagcctcc tgacctcaac gatcggtatt
     1981 tcggttccga catttgtcag cggattgatc ctgatcatct tcatgagtcg ccagttccat
     2041 gtctcgccga tcaagcggcc cgaggcatgg caggggctct tcagcactgc ctacatcctg
     2101 ccgggcatcg ttcttggtct cggcacgacc gcctacatca cccgcttgac gaggagcagc
     2161 atgctcgagg tcatgaagaa cgacttcgtg cgcactgccc gcgccaaggg actgagcggc
     2221 ggcgtggttg tccggcggca catgttgcgg aatgccctca tcccggtcat cacgatcatc
     2281 ggtcccgcaa cggcggacct cgtaaccggt tcgatcatca tcgaaaccat ctttggcgcg
     2341 ccgggaatcg gcaaggaatt cgtcacgtct attggtaagc gcgactattc cgtgattatg
     2401 gggattacga ttttctacgc ggtcctgatc gcaattgcga acgttttggt cgacctcagt
     2461 tacggcttca tcgacccccg gattcgccgc cgatgagtag cacagagctc gcaaagagcg
     2521 catccacctc tccggccacg gcagtcgccc cggccgcacc gagccttcac actatggatg
     2581 attctccgct cactgcgatg cggccacagc ggagcctctg gagcaatgcc tggcgccggt
     2641 tgcgcaagaa cctgatggcg atggctgcgc tttgctacct gttcttcctg ctgatggtcg
     2701 cgatcctggc gccggtgatc gcgccgcaca acccggtcaa gaccgacgtc gcccatgccg
     2761 gcacattgcg gaaagcggcg tgggtcgagg atccgaaccc cctgcgaacg ggcacctggg
     2821 attatccgct cggtaccgat gccgtcgggc gtgacgtctt cagccgcctc gtctacggga
     2881 cgcgcgtctc cctggtcgtc ggcttcatcc ccatggcagt catcttgctg atcggcgtgc
     2941 cggttgggtt gatcgcaggg tttgccggcg gcagagtcga taacttgctg atgcgaatga
     3001 ccgatgtcgt ctacgcattc ccggcactgt tattggcgat catcatgcag atctccttcg
     3061 gtgatacgag catcggcaag ctgttgaacg gtcttgttct gctcttcatt agcctctcaa
     3121 tcgtgaactg gaccggtgtc gcccgccttg tccgtggcga gacgctctcc ctcaaggaga
     3181 aggagttcgt cgaggcggcc cggacctctg gcgcatcgag gttccgcctg atcacccggc
     3241 atatcctgcc gaacgccctt gggccgatca tcgtcgccgg tgcgttcatc gttccgggcg
     3301 cgatcatcag cgaggcgatc ctgagctatc tcggcgtcgg cgtgcggccg gacgtcaacc
     3361 tgaatgcgcc gttcccgacc agctggggcc agatgatcct ggacgggtcg aagacctaca
     3421 actcgcaact ctggatgctg ctcgcgccga gcatcgccgt cgcgctgacg acgctctcgt
     3481 tcaccttcgt cggcgacggc ctccgcgacg cgctcgaccc gcgccagtcg gactagcggg
     3541 ctttgcgtga acggcaggac ggcccaacgt actcggacgc agatgtcgcg gcgctctacg
     3601 atgtcctgaa tcgctggggc ccgggcgacg acttttacct aacccttatt cgcgatgccg
     3661 aatcggtcct ggacgtcgga tgcgggaccg gcacgctcct gcaccatgcc cgccaggcgg
     3721 aacacactgg gcgattgtgc ggggttgatc cggatcaggc gatactggca gtggcgcgat
     3781 ctcaccgcgg catcgagtgg tgcgacggca ctgccgcatc gatgacctgg accggcgagt
     3841 tcgatctcgc cgtcatgacc ggtcatgcct tccagtgcct gatcacagac gaagaggtcc
     3901 gagcctccct ggtcgcaatc cgccatgcgc tggtcccggg cggccggttc gcgttcgaga
     3961 cacggaatcc gttggtccgt gcctgggagc actgggacgg tatggatccc atcgaggcag
     4021 tcgatccaca gggtcgggcg gtacgcgttt cctatgatgt ggaggacatc gtggaagacg
     4081 tggtcgcgct cgcggagatc acatgcgaca gcgaaaaccg gcgcctgcgg gtggacaggg
     4141 cacgcttgcg cttcatggac aaccaattat tgaccgattt ccttgacggc gcgggattcg
     4201 acattgaggc gcagtacggc aattgggacc gttccgccct cagcccatcc agccccgaga
     4261 tcatcacgat cgcgcgcacc cggtagccca tagatccgga caggaccacc ggacggaccc
     4321 cgtaattgac tgaaaactgg gtgaaaccta cacttcggcc atcgtcggat tacccttggt
     4381 cggggattgg ctgttttcga cggtcgaagg atgtccatgc cgcgcaccag caaggccacg
     4441 ctgatttctg agcaatccgc gtcggatggc atacccatcc ccacggcgct tcgtccccct
     4501 gccacatttc cggcggatcg cttgatcgag gtggcgcgct cccgattgca gagcgagctc
     4561 gtgcgacgca acgacggcgc tgtcccggcc gccattgaac gcgcggtaga ccgtgcgatc
     4621 gagtggcacg cggggcaagt tcgaaaatcc ggagagccgt acgtggtcca cccgattgag
     4681 gtggccattc ttttatcccg gatgtccgtc gattccgagg cgatcatcgc cgcgctgctc
     4741 cacgacgtcg tcgaggacag cagcgtcggg ctgagcacga tccgggagga gttcggcgat
     4801 cgcgtcgcgc tcctggtcga tggcgtcacc aagctcggtc aaatccagtg gaccggcgac
     4861 ggcgatcagg ccacccggga gcgggcggtc caggccgaga acctgcgcaa gatgttcctg
     4921 gcgatgatcg acgatatcgg cgtcgtcctg atcaagctgg ccgaccggct gcataacatg
     4981 cgcacgctcg accacatgtc gcgcgacaag caaatccgca tcgcgcagca gacgatggag
     5041 atctacgcgc cgcttgcgaa ccgactcggt atctggcagt tcaagtcgga gctcgaggac
     5101 ctcgccttcc ggtatctcca gcccgaggca taccagtcga tcaaggcccg gctcgaggag
     5161 cgcgggcggg atcaggccgc gtacatcgct caggtcatgc aggatctgaa agacgcgatc
     5221 caggcggagg gcatcgatgt cgagttgacc ggccgcacca agcacaccta ctcggtctac
     5281 cggaagatga agcagaagca gcggaccttc gacgagatct atgacgtaat cggcatccgc
     5341 tgcatcatcc ccgacgaccg gatcaaggac tgctacggcg tcctcggcgt catccattcg
     5401 atgtggcacc cgatcccggg cgagttcgac gactacattg ccacgcccaa gcagagcatg
     5461 taccagagcc tgcacacggc agtgatcgga ccgcaggggc atgcgctcga aatccagatc
     5521 cgcacccagc gcatgcacga gattgccgag ttcggcgtgg ccgcgcactg gcggtacaag
     5581 gaaggcaaga aggccaacgc caaggtcgag gcgaaggtcg cctggctccg gcagatgctg
     5641 gagtggcagg cggagatgaa ggacgccgag gatttcgtcg agacggtcaa gtccgacgtc
     5701 ctcgaggaga tgatctacgt cttcacgccg cggggggaca tcatcgagct cccggccggc
     5761 gcgactccgc tcgattttgc gtaccggatc cataccgaag ttggtcatca taccgtgcgc
     5821 gccaaggtca atgaccagct cgtccggctg gacacgaaac tcaagaacgg gcaagtcgtc
     5881 gagatttcga ccagcaagac caagctcggc cctagccgcg actggctgcg ggaagatctc
     5941 ggctatctga cgactgcatc ggccaaggaa aagatccggc agtggttccg ccggcaggag
     6001 cgcgaggaga acctggccca gggcaaggag atcctggaaa aggagctccg ccgcctcggc
     6061 gtggacctca agccggagga catctacaag cacttcccgc gctacagcaa ggtcgaggac
     6121 atgatcgcgg cgatcgggta cggcgcgatc tcgccgcggc tgatcgcggc tcgtctcggc
     6181 gagaccgaga ccaagaacgt cctctcggac acgccgcacg tcccgaagcc gcgcgttccc
     6241 ttgcggctcg acgtgatggg cgtcggcgac ctgctgacct cgctcgcgac ctgctgcaag
     6301 ccggtcaacg gcgactcaat cgttggctac gtgacccggg gacggggcat cacggtccac
     6361 cgggccgatt gctccaacct ggtcaacctg cccgatcctg agcggctgat ccaggtgacg
     6421 tggggctcga aggccggcga gacctacccg gtcaacatca tggtccgggc gtgggatcgc
     6481 gtcggcctgc tcaaggacat cagcacattg ctggctgatg agaaggtgaa catcctctac
     6541 gtccagacgg tcacgaatga cgatcggacc gtgaacctgc ggatcaccat cgaggtcgag
     6601 aacgtcagcc agctcagtcg cgtcctccac aagatcgaga gcgtcccggc ggtcgatgaa
     6661 gtccggcgcg acacgaacaa cggagcgacc gatactcgat tggccggcga ctgacgccgt
     6721 gaccgattcc cgcactgtgc tctgcctcgg tgaggtcctg atcgacatga tcgtgtcgga
     6781 cggctcaccg acgctcgagg atgccggcga gttcgtcgcg cggccgggtg gcgccccggc
     6841 aaacgtggcc gtcgccttgc ggcggttggg cgtgccgagc gccttttgtg gcgcggtcgg
     6901 cgacgatcca ttcggcgccc gattgcggcg agtgctaatc gcggacgggg tcgatacggc
     6961 aggtctgatg attgtgcccg ggcaggccac gacactggcc tttgcctgga aggatgagcg
     7021 tggggacggt catttccggc ttgtccgcct ggccgacgcc gctctttcgg cgctggcggt
     7081 cgagaacgcc gggattcccg agcgcgcggc catcatcgtc gggtcagtcg cgttgacaga
     7141 agaagactcc cggacggcga tctaccgtgc cgtcgaaatt gccggagatt ccggcgttcc
     7201 tgtctgcttc gacgtcaaca tgcggccgac cctgtggcgc tcggtcgagg atgcgcgggc
     7261 ggcctgccaa ccggtcatcg acatggtgac gatgctgaag ttgagcgtgg acgatgccga
     7321 aatcctgttc ggccgccggg agccggaggc gatctt
//
LOCUS       DASSGX010000324         2322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1254398, whole genome shotgun sequence.
ACCESSION   DASSGX010000324 DASSGX010000000
VERSION     DASSGX010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254398"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VFI12_05700"
     CDS             complement(<1..356)
                     /locus_tag="VFI12_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HET7055936.1"
                     /translation="MTTKTISPVTKSLEINGRTLTLETGLIAEQASGAVTIRYGDTQL
                     LVTVVGAPEPKENAAWFPLSVDYEERMYAAGKIPGGFIKREGRPSEKEILTSRQIDRP
                     IRPLFPEAFRNETQVVA"
     gene            complement(691..960)
                     /gene="rpsO"
                     /locus_tag="VFI12_05705"
     CDS             complement(691..960)
                     /gene="rpsO"
                     /locus_tag="VFI12_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HET7055937.1"
                     /translation="MALVKEHKTEIIKGFQVHEGDTGSPEVQVAILTERINQLTDHLR
                     GNKHDHHSRRGLLQMVGKRRRLLAYMNKKDPERYRTAIARLGLRK"
     gene            1073..1936
                     /gene="mtnP"
                     /locus_tag="VFI12_05710"
     CDS             1073..1936
                     /gene="mtnP"
                     /locus_tag="VFI12_05710"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HET7055938.1"
                     /translation="MTKLGVIGGSGLYNIEQMTDVRRALVPTPFGMPSDAAVIGRIGD
                     HEIVFMPRHGATHDLTPTEVPYRANIAAMKQLGVTHLLSVSAVGSLTERYPPLTLVCP
                     DQIIDRTVLRDRTFFDNGVVAHVGLADPFCSTFSDQIIDRAAKAGVTLERGGTYVAIE
                     GPQFSTRAESELYRSWGAAIIGMTAMPEARLAREAELHYAQLAMVTDFDVWHESEAPV
                     TVETVVANLHQNSANAKRVLLAILDGALEQSDSCGCTTALKHAIITAPDKISAEQRER
                     LSVIAGRYLQS"
     gene            1940..>2322
                     /locus_tag="VFI12_05715"
     CDS             1940..>2322
                     /locus_tag="VFI12_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055939.1"
                     /translation="MLWRLQRELSLTLFAAALLIGGYAASWANLHSVATISIGQVRTA
                     AILAAVLLLVPMVWAGARMAGDEVIFPICALLLAIGLISMRRLQPDIGGPNSDLGNLS
                     GRHTLYVVLSVIVMTATGRWFPFWG"
BASE COUNT          408 a          740 c          707 g          467 t
ORIGIN      
        1 gccacaacct gcgtctcatt ccggaaggcc tccggaaaca gcggacgaat cgggcggtcg
       61 atctgccgcg aagtcagaat ctccttttcg ctcggccgtc cctcgcgctt gatgaagccg
      121 cccggaatct tgccggccgc gtacatccgc tcttcgtaat ccaccgagag cgggaaccag
      181 gctgcgttct ccttcggctc cggcgcgccg acaacggtca cgagcagttg cgtatcgccg
      241 tagcgaatcg tgacggcgcc actcgcctgc tcggcgatca ggccggtctc gagcgtgagg
      301 gtacggccgt tgatttccag tgacttggtc accgggctaa tagtcttggt agtcatgttg
      361 tttggttttc tccaccatac attccgctgc cgccgaattg cgggcaagcg gcgaacaagt
      421 cgactggtgg cggacgggag aaaggaactg gagtgcgaag gccggtctcg tggcgccctc
      481 cgcgctccaa tacctccctc accgcgcgcg tcaccaggtg ttatcggttc atttgtacgt
      541 caggcagtct cctttcgatg tcatcttcgc gacacaacaa aacccggcga aggtcagact
      601 ccgccgggat tcgcattgca ctcggggtcg gagcagccaa ccgcgccatg cgcggcgacc
      661 gcaccaggag gtatcggacc ggggaaccgc ctatttgcgg agccccagac gagcaatcgc
      721 cgttcggtat cgctcgggat ccttcttgtt catgtaggcc agtagccggc ggcgcttgcc
      781 gaccatctgc agcagcccac ggcggctgtg atgatcatgc ttgttgcccc gcaagtggtc
      841 ggtcaactgg ttgatccgct ccgtcaggat cgcaacctgg acctccggag atccggtatc
      901 gccctcgtgg acctggaagc ctttgatgat ctcggtcttg tgctctttaa ctaacgccat
      961 gttcggcgct gctccctttg ccgcaaggca tgcgtaaatc tgctgcacgc tcgaattgtc
     1021 acagcgaacc agtataccat cccgctgttg atctggatag cgagggcaac cggtgacaaa
     1081 actcggcgta atcggcggaa gtggactgta taacatcgag cagatgacgg atgtgcggcg
     1141 cgcgctcgtg ccgacaccat tcggaatgcc aagcgatgcg gcggtgatcg gccggatcgg
     1201 tgatcacgag atcgtcttca tgcctcgtca cggagctacc catgacctga cgcccaccga
     1261 agtcccgtac cgggcgaata ttgcggcgat gaagcagctc ggcgtcacgc atttgctcag
     1321 cgttagcgcc gtcggcagcc tgaccgagcg ctacccacct ttgactcttg tctgcccgga
     1381 tcagatcatc gatcggaccg tcctccgtga ccggacattc ttcgacaacg gcgtcgtcgc
     1441 gcatgtcggt ctggctgatc cgttttgttc caccttctcg gaccaaatca ttgaccgggc
     1501 agcaaaggcg ggagtcacgc tggagcgcgg cgggacctac gttgcgatcg aaggaccgca
     1561 gttctcgacc cgcgccgaat ccgagcttta tcggtcgtgg ggagcggcga tcatcggcat
     1621 gacggccatg cccgaggccc gtttagcgcg agaagccgag ctccactacg cccagctcgc
     1681 gatggtgacc gatttcgacg tctggcacga gagcgaggcg ccggttacgg tcgagacggt
     1741 cgtcgcgaat ctgcaccaga actcggcgaa cgcaaaacgg gtcctgctcg cgatcctgga
     1801 tggcgccctc gagcaatccg actcctgcgg ctgcacgacg gcgctcaaac acgcaatcat
     1861 cacggccccc gacaagatct cggccgagca gcgcgagcgg ttgtcggtca tcgcgggccg
     1921 atacctccag agctgatctg tgctctggcg actgcaacgc gagcttagcc tgactctgtt
     1981 cgccgcggcg ctgctgatcg gcgggtatgc cgcaagctgg gcgaatctgc actcggttgc
     2041 gacgatttcc atcggccagg tgcggacagc cgcaatcctg gcggcagtcc tcctgctcgt
     2101 gccaatggtc tgggccggcg cccgcatggc cggcgacgaa gtgattttcc cgatctgcgc
     2161 gttgttgctg gcgatcgggc tcatttcgat gcgccgcctc cagccggaca tcggcggtcc
     2221 gaattcggac ctcggaaacc tctccggccg gcacacgctg tacgtcgtgc tttcggtcat
     2281 cgtgatgacc gccacgggcc gttggttccc cttctggggg tt
//
LOCUS       DASSGX010000325         2011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1474690, whole genome shotgun sequence.
ACCESSION   DASSGX010000325 DASSGX010000000
VERSION     DASSGX010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1474690"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..436)
                     /locus_tag="VFI12_05720"
     CDS             complement(74..436)
                     /locus_tag="VFI12_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET7055940.1"
                     /translation="MPRFVAVYTMKPEDLAAFRAQAKSEQEATDKLGLKAWKEWGERN
                     AAAIVATDVMVGKTKRVTKAGIADARNQIAGFLIVEAADIAAAARLFQDHPHITVFPG
                     DGIDVMPVVTEPPEDEPP"
     gene            826..1710
                     /locus_tag="VFI12_05725"
     CDS             826..1710
                     /locus_tag="VFI12_05725"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET7055941.1"
                     /translation="MVGAGVKVATFEWRGKRAVGLVRPEEGTVASFDLPEEAMREGVS
                     CLIGLDRQPRLLDPVALSEVTLQAPLPRPRRNIFCVGKNYYEHAHEFSKSGFDSSAAA
                     GAVPAHPIVFSKVPESVIGPSAPILIDPAVSTAIDYEAELAVIIGRAGRGIRPENAFD
                     HVWGYTIINDVTARDLQGRYSQWLIGKSQDTFCPMGPWAVTRDAFDISNADIRSRVNG
                     ELRQESNTGRLIFDVPTIIATIAAGVTLLPGDVIATGTPAGVGIGFDPPKYLAPGDIV
                     SVEVEGIGVLENSCQLMV"
     gene            complement(1763..>2011)
                     /locus_tag="VFI12_05730"
     CDS             complement(1763..>2011)
                     /locus_tag="VFI12_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7055942.1"
                     /translation="KFLLLDEPSQGLAPVIVDIVVDTLVRLRNEKIGILLVEQNAEIA
                     LEIGDEATIIEQGEIVHRGTAAETRANPDVMTAHLSVG"
BASE COUNT          329 a          614 c          670 g          398 t
ORIGIN      
        1 gaagggtaga cactcgcgac gggcatcgca tagacgcggc gccagttggc ggcggacctg
       61 gctgttgctt gcttcacggc ggctcgtcct ctggtggctc ggtgacgacc ggcatgacgt
      121 cgatgccgtc gccggggaag acggtgatgt gagggtggtc ttggaagagg cgggcggcag
      181 cggcgatgtc ggcggcttcg acgatcagaa agccggcgat ctggttccgc gcgtcggcaa
      241 tgccggcttt ggtgacacgc ttggtcttcc ccaccatgac gtcggtcgcg acgatggcgg
      301 cggcgttgcg ctcgccccac tccttccacg ccttcaagcc gagcttgtcg gtcgcctcct
      361 gctcggattt ggcctgcgcg cggaaagcgg cgaggtcctc cggcttcatg gtgtagacgg
      421 cgacgaagcg aggcatgggc gtactcgaga tttggtgccg ggttgcgttg acgaaggtgt
      481 ctcgcccgat gtgccgggtg atggtgccgt cctctttcgg cagctcgaaa gcgcgcctga
      541 cagtgttgcg cgcaaccctg aactctcgcg catgatcccg ttccggagga atccgtccgt
      601 ctccgcccac tgccccgcgg cgatccgctt acgcagggtc gcggcaatct cgtctgctct
      661 aatgcgcgcc atggcagagt tgtgaggagg actgagccaa gaatgatccg attagcatcg
      721 gcttatctgg gcagtccagc caaaagcctc gacctcttgg tgaggctggc agtgattgtc
      781 tctctattgc cattggtcta acaatgcgcc gggaacggtt cagtaatggt gggggctggt
      841 gtgaaggtcg cgacgttcga gtggcgaggg aagcgggctg tcgggctggt caggccggag
      901 gaggggacgg tcgcttcttt cgatcttccc gaggaagcga tgcgcgaggg cgtgtcgtgc
      961 ctgatcggcc tggatcgcca gccgaggctc ctcgatccgg tggcgctgtc ggaggtgaca
     1021 ctgcaggcgc cgcttccacg gccgcgccgg aacatcttct gcgtcggcaa gaactactac
     1081 gagcacgctc acgagttctc aaaaagcggg ttcgattcca gcgccgcggc cggcgccgtg
     1141 ccggcccatc cgatcgtctt ttcaaaggtg ccggagagtg tcatcgggcc ttccgcgccc
     1201 atcctgatcg acccggcggt ctcgaccgcg atcgactacg aggcggagct cgccgtcatc
     1261 atcgggcggg ccgggcgagg catccgtccg gagaatgcct tcgaccatgt ctggggctac
     1321 accatcatca acgacgtgac cgcgcgcgat cttcaggggc ggtacagcca gtggctgatc
     1381 ggcaagtcac aggacacatt ctgcccgatg ggcccctggg cggtaacgcg ggacgcgttc
     1441 gacatttcca acgcggatat ccgcagccgc gtgaacggcg agctgcgcca ggaatccaat
     1501 accggacggc tgatcttcga cgttccgacc atcattgcga cgatcgccgc gggcgtcacc
     1561 ctgctgccgg gcgacgtcat cgccaccggc actccggccg gcgtcggcat cggcttcgat
     1621 ccgccgaagt atctcgcacc cggcgatatc gtcagcgttg aagttgaagg tatcggggtc
     1681 ctggagaatt cctgccaact catggtctga tgagtggacc ggttacatcg aaacggaacg
     1741 gccccagccg gttgtttctg gtctaaccaa cggagagatg cgccgtcatg acatcggggt
     1801 tggcccgcgt ttctgcggcc gtgccgcggt gaacgatttc gccctgctcg atgatggtcg
     1861 cctcgtcgcc gatctcgagc gcgatctcgg cgttctgctc gaccagtaga atgccgattt
     1921 tctcgttcct cagccgcacc agcgtgtcga ccacgatatc tacgatcacc ggcgcgagcc
     1981 cttgtgaggg ctcgtccagg agcaggaatt t
//
LOCUS       DASSGX010000326         2817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_492857, whole genome shotgun sequence.
ACCESSION   DASSGX010000326 DASSGX010000000
VERSION     DASSGX010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492857"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VFI12_05735"
     CDS             complement(<1..446)
                     /locus_tag="VFI12_05735"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET7055943.1"
                     /translation="MSARQTAINTAVILLVVLVAWLLVQVRSTLVLLIIGILFAAAIE
                     PLVNRLRRRGLKRGQAILLIYAGILAAVFLALLLAVPEIVRQTTSLIDNIPDILQSAR
                     ERAAEIKSTAIRNAALRGINEVDDFYIDTRTNPDAGLASNAAIVLLT"
     gene            complement(452..736)
                     /locus_tag="VFI12_05740"
     CDS             complement(452..736)
                     /locus_tag="VFI12_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055944.1"
                     /translation="MSEEHAWMRPTLEAMFASVDLETAREAVLSDVRRGELRSQLNRE
                     LDDDAQIPDGQYADNLLQGLLALRMKFDRDEAMRKLELNLAMRAERRNRP"
     gene            complement(733..1014)
                     /locus_tag="VFI12_05745"
     CDS             complement(733..1014)
                     /locus_tag="VFI12_05745"
                     /inference="COORDINATES: protein motif:HMM:NF016230.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET7055945.1"
                     /translation="MSDGETYLCRCECITTEQVDAAIAEGNVTTNDVKRRTRAGMGIC
                     QGIFCLSAMAERLQAAGVDAGAIEPMTSRPPTRLITVGLAASHDAEQDA"
     gene            complement(1011..1904)
                     /locus_tag="VFI12_05750"
     CDS             complement(1011..1904)
                     /locus_tag="VFI12_05750"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7055946.1"
                     /translation="MSVAIVGAGPAGMIAATLLADRGGKVTLIDEQSAFGGHLTHDRY
                     TVGMDGHDSDSWLAEMRSALDSASVTSLPLAIAWAAFRNGDGFALGVNHAGAEKTVVT
                     DHLVIAAGTTDLPLIAPGATLPGVLTGRAIRILVNRHGVIPGQRIVVVGVGPKADRLR
                     DDLIAVGCDVVAQLAVAEVAAITGNGGVQEVQTADGRTLDADLIGIAVGEVPDVQLAG
                     MVEAPRVFDPNLNGWRLASEGVPAGLHVIGGSLLGQATPEEIVQSAVAVADRIRPGGE
                     NLRDVGLDISDAILRQEAVQR"
     gene            complement(1901..2221)
                     /locus_tag="VFI12_05755"
     CDS             complement(1901..2221)
                     /locus_tag="VFI12_05755"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET7055947.1"
                     /translation="MNAHGARRRATPTGSAVQITFDGEPVSALETDTVASALLAAGYR
                     TFTIDPHDGSPRGGFCFVGRCSDCLMVIDGQPGTMACITPVRDGMRVETQQGRGHWGV
                     GREA"
     gene            2388..>2817
                     /locus_tag="VFI12_05760"
     CDS             2388..>2817
                     /locus_tag="VFI12_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7055948.1"
                     /translation="MATTVASKTSQAEMDPTAPAYLNKEAMDHVWIHSVPWIELAERN
                     GLHVFDRGEGSTIYDVNGNAYLDGIAGLWVVNAGHGRKEIAEAMGRQAARLAYVSSMN
                     FTTVPAVQLADMIAERTPGDLNRVFFAAGGSEAVESAIKIA"
BASE COUNT          496 a          960 c          881 g          480 t
ORIGIN      
        1 gtcagcagca caatcgctgc attgctggcg aggcccgcgt cggggttggt gcgggtgtcg
       61 atgtagaagt cgtctacttc gttgatgccc cgcagcgccg cgttcctgat ggcggtgctt
      121 ttgatctcgg ccgcccgctc gcgcgccgac tggaggatgt cggggatgtt gtcgatcagc
      181 gacgtcgtct gccggacgat ctcgggaacg gccagcaaga gggcgaggaa gaccgccgcc
      241 aatatgcccg catagatgag caggatcgcc tggccgcgtt tcagcccgcg ccgacgcagg
      301 cgattgacca gcggctcgat cgcggccgcg aacaggatcc cgataatcag caggaccagc
      361 gtcgaccgca cctgaaccag cagccaggca accagcacga cgagcaagat gacggcggtg
      421 ttgatcgccg tctgccgggc cgacatgggc gtcacggacg gttccttcgc tcggcgcgca
      481 tggccaggtt cagctcgagc ttacgcatcg cctcgtcgcg gtcgaacttc atgcgcaggg
      541 cgagcaatcc ctggagcagg ttgtcggcat attggccgtc cggaatttgc gcatcatcgt
      601 cgagttcacg gttgagctgc gaccgcagct ccccgcgccg gacatcgctg agcaccgctt
      661 cccgggcggt ctcgaggtcg accgaggcga acatggcctc gagcgtcggc cgcatccagg
      721 cgtgctcctc gctcacgcgt cctgctcagc atcgtgagag gcggcgagtc cgaccgtgat
      781 cagacgggtc ggcgggcggg aggtcattgg ctcgatcgca ccggcatcga cgccggccgc
      841 ctgtaaccgc tcggccatcg cgctcaggca gaagataccc tggcaaatgc ccatcccggc
      901 ccgggtgcgc cgcttgacgt cgttggtggt cacgttgcct tccgcgatcg ccgcatcgac
      961 ctgctcggtc gtgatgcact cgcatcggca caggtatgtc tcaccatcgc tcaccgttga
     1021 accgcctcct gccgcaggat cgcatcggat atatcgagcc cgacgtcccg caaattctcg
     1081 ccaccgggac gaattcggtc cgccacggca accgcgcttt gcacgatctc ctccggcgtg
     1141 gcctgcccca ggagtgagcc accaatcaca tggagaccgg ccgggactcc ttcggaagcg
     1201 agtcgccagc cattcaggtt gggatcgaag acgcgcggcg cctcgaccat cccggccagt
     1261 tgcacgtccg gaacttcgcc gaccgcgatt ccgatcaggt cggcgtcgag tgttcggccg
     1321 tcggctgtct gaacctcttg cacgccacca ttgcccgtaa tcgccgcaac ctcagcgacg
     1381 gcgagctggg ccactacatc gcagccaacg gcgatgagat cgtcccggag ccggtctgct
     1441 ttcgggccaa ccccaacgac cacgatccgt tgccccggga tgacaccgtg gcggttcacc
     1501 agaatgcgga tcgcccgacc ggtcaacacg cccggcaagg tcgcgcccgg agcgatcagc
     1561 gggaggtccg tcgtaccagc cgcgatgacc aggtgatcag taacgaccgt tttctccgcg
     1621 ccggcgtggt tcacccccag cgcgaagccg tcgccattcc gaaacgctgc ccaggcgatt
     1681 gccaggggca gggatgtcac gctggcggag tccaacgcag agcgcatttc cgctagccaa
     1741 ctatccgagt cgtgcccgtc cattccgacc gtgtaccggt cgtgggtgag atggccaccg
     1801 aatgccgact gctcgtcgat cagggtgacc tttccgccgc ggtccgccag gagggtggcc
     1861 gcgatcatcc cggcgggacc ggccccaaca attgcgacgc tcatgcctct ctcccgacgc
     1921 cccagtggcc acgcccctgc tgcgtctcga cccgcatccc gtcccgtacc ggcgtgatgc
     1981 acgccatcgt gcccggctgc ccgtcgatca ccatcaggca atcgctgcat cggccgacga
     2041 agcagaaccc gccacgtggt gagccgtcgt gcggatcgat cgtgaaggtg cgataccctg
     2101 ccgcgagcag cgcgctggcg acagtgtcgg tttccagagc cgaaactggt tcaccatcaa
     2161 atgtgatctg aacggcgctg ccggtcggcg tggcccgcct gcgggcccca tgtgcattca
     2221 tccccaacat tcccggtaat agtgagctca tctggcgcgt catattgtca ttccgggcgg
     2281 agagcgtcaa acggaatccc ggggcgctcg gctatactca ggcagtttca caccaatccg
     2341 ccaacggctg ccggattgac agccctgggc tcgaggagaa gacctttatg gcaaccacag
     2401 tcgcgtcgaa gaccagtcag gcggagatgg atccaactgc tcccgcctat ctgaacaagg
     2461 aggccatgga tcacgtctgg atccattccg tgccgtggat cgagctcgcc gagcggaacg
     2521 ggctgcacgt cttcgatcgc ggtgaaggca gcacgatcta cgacgtgaac ggcaatgcct
     2581 acctcgacgg catcgccggc ctgtgggtcg tcaacgccgg gcacggacgc aaggaaatcg
     2641 ccgaggccat gggccgccag gccgctcgcc ttgcctacgt ctcatccatg aacttcacga
     2701 cggtaccggc ggtccagctc gccgacatga tcgccgagcg cacgccgggc gacctcaacc
     2761 gggtcttctt cgcggccggc ggttcagaag cggtcgagtc ggcgatcaag atcgcga
//
LOCUS       DASSGX010000327         8427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_385115, whole genome shotgun sequence.
ACCESSION   DASSGX010000327 DASSGX010000000
VERSION     DASSGX010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8427
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385115"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1268
                     /gene="lhgO"
                     /locus_tag="VFI12_05765"
     CDS             <1..1268
                     /gene="lhgO"
                     /locus_tag="VFI12_05765"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HET7055949.1"
                     /translation="LPVARATTVERRESGMANSDVVVIGAGIVGLATAREILLRRPGT
                     QLTVLEKEADIAQHQTGHNSGVIHAGIYYKPGTLKARLCVAGGADLTCYCDEQGIPYQ
                     LCGKLIVATDEGELPRLADLERRATANGCQGIRIVEAEEIREIEPHVRGIKALWSPRT
                     GIIDYGVVARHYAQDVNNMGGEIRTSSPVTGIQRGTGDTTVQTEQGDFTARHVIACAG
                     IYSDRVARMTGASKLPLIAPFRGDYYVLRPERRDLIRTNIYPVPDPQFPFLGVHFTPR
                     MNGDVWLGPNAVLAFSRTGYRFRDVNPRDMAQLFTSPGFLKFGRKHWRTGIDEMARDL
                     SKKRFLASLQRYVPELTEEDLLPGPSGVRAQALTSAGEMVDDFVIDRQEGILHVRNAP
                     SPAATSSLQIGAYIADALAEMDDRYAARA"
     gene            complement(1270..2490)
                     /locus_tag="VFI12_05770"
     CDS             complement(1270..2490)
                     /locus_tag="VFI12_05770"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7055950.1"
                     /translation="MTESTGIRRHAGLLLLLAAGGFTSALNVTLLSPLIVAIARDFDV
                     SEAAAGQLATLTAASSGVMAVVVAPWMDRYPRRFWLRLECGLLATGTLITALAPAFAW
                     MFLGRMLCGLGGAVIGATCLASCGDIFPDSTDRNRALGMINSAFTLGATIGLPLVAMT
                     ADWTDWRVAIVIPAPLALLVLIGTRRMPVVRRRAVGSIWEAWRAGYGRVLRSRETVWL
                     LASEILLQVVWFGWLIYFGAFTDTVYGISAALLSLLFFVGGGAEIIANNLTPILTRNR
                     SITAVAYPAVAILALNLLMIGIALDQKWTLFPFIAFGSLTGAALLLCFNIALLDSLPE
                     DPGAVMSLQSGSFEFGGSVGVALTGIALALIDDYEAVYRLLGVVTPLIAVTFWLSTRA
                     RSAERQPAAASAGI"
     gene            complement(2550..3419)
                     /locus_tag="VFI12_05775"
     CDS             complement(2550..3419)
                     /locus_tag="VFI12_05775"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7055951.1"
                     /translation="MIRRLWLLAALLFLSLGLLAPSAIAQDATPAAVAPPQDLLPPFT
                     DEVAGQTVEINGANIYYEVYGTGEPLILIHGGLGNGKYWVNQIPVFAAKYQVIVMDSR
                     GHGRSSFDEQPITYALMASDVLGLMDHLAIDKASIVGWSDGGIIGLEIAIHHPERLNK
                     VVAYGANFDPTGVRLDIGDNAYFNAYIERASEDYLTLSPAPERWDEFLNNIGTMWATL
                     PNYTEDELMAITTPFLILDGETEEAIDLNQTKLMALLMPNADLKLMPGTGHFAMFEQP
                     DEFNAIVMDYLAP"
     gene            3868..5121
                     /locus_tag="VFI12_05780"
     CDS             3868..5121
                     /locus_tag="VFI12_05780"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET7055952.1"
                     /translation="MTGSQIGGRYELLETIGEGGFAVTWRAWDTRLERAVALKLLRPQ
                     YASDERFVVRFVREARAAASVSHPNVVRLYDFGQESRAVYLAMQYVQGQTLRDLSRRF
                     PGGMPERDAVGLIAPVLRGLSAIHAAGIVHRDVKPDNVLIDRDGDVLITDFGIALLTD
                     STRLTAADTTFGTAAYMAPEQGRGEPVSAATDLYATGVMLFEMLTGRLPFTAENPVAM
                     MMAHQDQSPPRPSQIAPGNAISPAVEGAVMRVLAKRPADRLQSASSFLDALSGRAAAP
                     TTRGAPPTATMPIQRAPRIQPAAVPQRPVRQPAPVRRSSAWSWLLVVLVIAGVAAAGA
                     AYATGYFDSSDAPNQNAPVVEPTATDTPIDEPTQSAIEGPTIAPVEEATEPVETPTPV
                     FAEEPTDTPESSPHVPIEPINGAGP"
     gene            complement(5129..6106)
                     /locus_tag="VFI12_05785"
     CDS             complement(5129..6106)
                     /locus_tag="VFI12_05785"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HET7055953.1"
                     /translation="MRTRFTELLGITHPIVSAPMAGSAYAELVAAVSAAGGFGLIGAS
                     HATAKPERPEWLRAQIRQIRERTDRPFGVGLALCFPQFDELLAVSLAEGVAGVAISFG
                     DVIPYVERVKAAGAVLLSQVQTVAEAKRLAVAGVDVLAAQGVEGGGHIGEISTFTLLP
                     AVRDAVDVPVLAAGGIADGRGLAAAIMMGADGAWMGTRFVASTESASGKWHKDAIVAA
                     GADDTVRTLAYDLARNVPFPEGIAGRAIKNRFTEQWHGRDDEIVSRREELDAEFAAAI
                     SSGTAEWSAAWAGSASEVIHSIEPAGELVRRITADAESILGRRPSAILD"
     gene            complement(6106..7464)
                     /locus_tag="VFI12_05790"
     CDS             complement(6106..7464)
                     /locus_tag="VFI12_05790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET7055954.1"
                     /translation="MPTLIIPVESQPRVQRRAIPIAARWRTFAAIALLSAVTLWHLWP
                     AVATTPFHRDEARWIGNSAVLREWRHPLGEKWQDEGYRNEYGTIDEVNRRRSQPPLAM
                     YLFGIGLTVQGEGLPQTGYWIMDKDTEWNTAHGNMPSGSELRAARRTNVVVALLTVLI
                     LYAIGARLTNRLGGVIAGLAYALHPLVTETSTRAFSDPLVVLCIVAAAGAAIRFGARP
                     TFGRAALIGVLLGLGAATKLSPLLLAAAIGALGIAPIAWGVATRRRVALRSGLAIGLI
                     PIVAGLTFFALYPYLWTDPVTHAQRMFEFRELSFDWQADVSPHARVIGLGDATRRFGV
                     QLGERESTAGYLLSELRRRVDVGDWLWFRELDLMLAAAGWILLFVLLVRRRFPADQVM
                     PLLVLGGQVALIAATFRLDYARYMLPLLPAIAIGIGAIAGLAWQHANGLVAARRIRAE
                     ER"
     gene            7657..>8427
                     /locus_tag="VFI12_05795"
     CDS             7657..>8427
                     /locus_tag="VFI12_05795"
                     /inference="COORDINATES: protein motif:HMM:NF015686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HET7055955.1"
                     /translation="MDWISNPEIWTALLTLTALEIVLGVDNIIFISILAGKLPVHQRD
                     RARILGLAAAMLTRILLLFTLNWIIGLTRTLFTVFGEDISGRELILLFGGLFLLGKST
                     FEIHEKLEGAEEHSAQRTGASFASVIIQIMLLDIVFSLDSVITAVGMAKHIEVMVAAV
                     VISVGIMLIASGPISRFVDQHPTVKILALSFLMLIGMSLVAEAFEQHVPKGYIYFAMG
                     FSVLVEVLNLRIRKKDVEPVQLHNPRMNEEIVDAAARVG"
BASE COUNT         1501 a         2852 c         2710 g         1364 t
ORIGIN      
        1 tactgccggt ggcgcgtgca acgacggtgg aaaggcgtga atcgggaatg gcgaacagcg
       61 acgtggtggt gatcggggcg gggatcgtcg ggttggcgac ggcgcgggag atcctgctcc
      121 gccggccagg cacgcaactg acggtcctcg agaaggaagc cgacatcgcc cagcaccaga
      181 ccggccacaa cagcggcgtc atccacgccg gcatctacta caagccgggc acgctcaagg
      241 ccaggctgtg cgtggcgggc ggcgccgatc tgacgtgcta ttgcgacgag cagggcatcc
      301 cgtaccagct ctgcggcaag ctgatcgtgg ccaccgacga gggcgagctg ccccgcctgg
      361 ccgacctcga gcgccgggcc accgccaacg gctgccaggg cattcggatc gtggaggccg
      421 aggaaatccg cgagatcgag ccgcacgtcc ggggtatcaa ggcgctctgg tcgccccgca
      481 ccggaatcat cgactacggt gttgtcgccc gccactatgc ccaggatgtc aacaacatgg
      541 gcggcgaaat ccgtaccagc agtcctgtca caggcatcca gcggggcacc ggcgacacga
      601 ccgtacagac ggagcagggc gatttcaccg ccaggcacgt cattgcctgc gccgggatct
      661 actcggatcg cgtcgccagg atgaccggcg cttcgaagct gcccctgatc gctccctttc
      721 ggggcgacta ttacgtgctt cggcctgagc gccgcgatct gatccgaacc aatatctatc
      781 cggtgccgga tccgcaattc ccgttcctgg gcgtccattt caccccgcgc atgaacgggg
      841 atgtctggct cgggccgaac gctgtgctgg cattctcgcg gaccggatat cggttccggg
      901 acgtcaaccc gcgcgacatg gcccagctct tcaccagccc cggctttctc aagttcggcc
      961 gcaagcactg gcgaaccggg atcgacgaga tggcgcgcga cttgagcaag aagcggttcc
     1021 tggcctcgct ccagcgctac gtgccggagt tgaccgagga ggatctgctg cccggcccat
     1081 cgggagtacg cgcccaggcc ctcaccagcg ccggcgagat ggtcgatgac ttcgtgatcg
     1141 accggcagga gggcatcctg cacgtccgca acgcgccctc accggctgcg acatcctcgc
     1201 tccagatcgg cgcgtacatc gccgacgcgt tggcagagat ggacgaccgg tacgccgccc
     1261 gggcctgact cagatgccgg ccgacgccgc tgccggttgc cgttcggccg atcgcgcccg
     1321 ggtgctaagc cagaacgtga ccgcgatcag cggcgtgacc acaccgagca accggtagac
     1381 cgcctcgtag tcgtcgatca gagcgagcgc gatgcccgtc aatgccaccc cgactgaacc
     1441 gccgaactcg aagctccccg actggagcga catcaccgcg cccgggtcct ccggcagcga
     1501 gtccagcagc gcgatgttga agcacagcag cagggccgcc ccggtcaggc tgccgaacgc
     1561 gatgaatggg aacagcgtcc acttctggtc gagagcgatg ccgatcatca ggagattcaa
     1621 tgccaggatt gcgaccgccg gataagcgac cgccgtgatc gaccggttcc gggtcaggat
     1681 cggcgtcagg ttgttggcga taatctccgc cccgcccccg acgaagaaga gcagcgagag
     1741 tagcgccgcc gagatgccgt agaccgtgtc ggtaaaggcg ccgaaataga tcagccagcc
     1801 gaaccagacc acctggagca ggatctcgga ggccagcagc cagactgtct cccgggatcg
     1861 cagcacacgc ccgtacccgg cgcgccacgc ctcccagatc gatcccacgg cgcggcggcg
     1921 gaccacgggc atccggcgcg tgccgatcag gaccagcaat gcgagtggcg ccgggatcac
     1981 gatcgccacc cgccagtcgg tccagtcggc cgtcatcgcg accagcggca gtccgattgt
     2041 cgcgccgagc gtgaaggcac tgttgatcat gccgagcgcc cgattgcggt cggtcgaatc
     2101 cgggaagatg tcgccgcacg aagccaggca ggtcgcgccg atgaccgccc cgcccagccc
     2161 gcagagcatc cgcccgagaa acatccatgc gaatgccggc gcaagggccg tgatcagcgt
     2221 cccggtcgcc agtagcccgc attccagccg gagccagaag cggcgcggat accggtccat
     2281 ccacggcgcg acgacgaccg ccatcacccc cgagctggcg gcggtcaggg tcgcaagttg
     2341 accggctgcg gcctcgctta cgtcgaaatc ccgcgcgatc gcgacgatca gcgggctgag
     2401 caaggtcaca ttgagcgcgg aggtgaatcc gccggccgcg aggagcagga gcagccccgc
     2461 atgccggcga ataccggtcg actcggtcat gacaagactc ggttggcggc gtcccggcgc
     2521 ataacgagct gccgggacac ctggatgcgc tacggggcca ggtaatccat cacgatcgcg
     2581 ttgaactcat cgggctgctc gaacattgcg aagtggcccg tgccgggcat cagcttcaaa
     2641 tccgcgttcg gcatgagcag cgccatcagc ttggtctggt tgaggtcgat cgcctcttcc
     2701 gtttccccgt cgaggatcag gaacggcgtc gtgattgcca tgagctcgtc ctcggtgtag
     2761 ttcggcaatg tggcccacat cgtgccgata ttgttcagga actcgtccca tcgctccggc
     2821 gccggcgaga gcgtcaggta atcctcggat gcgcgctcga tgtaggcgtt gaagtaggcg
     2881 ttgtcgccga tgtccagccg gacgcccgtc ggatcgaaat tcgccccata cgccacgacc
     2941 ttgttcagcc gctccgggtg gtggatcgcg atctcgagac cgatgatgcc gccgtcgctc
     3001 cagccgacaa tcgaggcctt gtcgatcgcg aggtggtcca tcagccccag cacgtcggat
     3061 gccatcagcg cgtaggtgat cggctgctcg tcgaaggagg agcgcccgtg cccgcggctg
     3121 tccatcacga tcacctggta cttcgccgcg aagaccggga tctggttcac ccagtacttc
     3181 ccgttgccga ggccgccgtg gatcaggatc aatggctcgc cggtgccgta gacctcgtaa
     3241 tagatattcg cgccgttgat ctcgaccgtc tgccccgcga cctcatccgt gaagggaggc
     3301 agcaagtcct gcggcggcgc gactgccgcc ggagtggcat cctgggcgat cgccgaggga
     3361 gccaggagcc cgagcgagag gaacaggagc gctgcgagaa gccagagtcg ccgaatcatg
     3421 gggacctcct gcgatttcgg ttgcgcatca cccggtcggt ggacgggaac atgcgcatta
     3481 tcgggccgga ttggaggctg tcaagaggaa tatgccggta gcacgcagta cgtccgctga
     3541 tgggccactg cccgtaggat gctttcgtta tcagtggatt ggcggccgat gggagcccca
     3601 atcatcgggt cgaggaggac tgtgatatgc gaattggcgg tggcgcgctc gcgctgatca
     3661 tcatcattct cttgctcatc tggctactct agagctggtc agaattggca atgcggagcg
     3721 cccggcgaac actgccgggc gctctcgtgt ctgcgtccgc aaatgcgccc cggctccccg
     3781 ctcgcctacc attagctgcc gaagcggggg agggcctcgt attcgggttg tgacaggcgg
     3841 cgccggttcg ggaccgtcca gagagacatg acgggttcgc aaatcggtgg ccggtacgaa
     3901 ttgctcgaga cgatcggtga gggcggtttc gccgtcacct ggcgcgcgtg ggatacccgt
     3961 ctcgagcgtg ccgtcgccct gaagctgctg cggccgcagt acgcctccga cgagcgcttc
     4021 gtcgttcgtt tcgtccgcga agcgcgcgcg gccgcatcgg tctcgcaccc gaatgtcgtc
     4081 cggctctacg acttcggcca ggagtcgcgc gccgtctacc tggccatgca atacgtccag
     4141 ggtcagaccc tgcgagacct gtcgcgccgc ttcccgggcg gcatgccgga gcgggacgcg
     4201 gtcggcctga tcgcgccggt gctgcgcgga ctctcggcga tccacgctgc cggcatcgtc
     4261 caccgcgacg tcaagcccga taacgtgctg atcgaccgcg acggtgatgt gctgatcacc
     4321 gacttcggca tcgcgctcct gaccgacagc acccgcctga ccgccgccga caccaccttc
     4381 ggcacagcgg cctacatggc gcccgagcag ggccggggcg aaccggtcag cgccgcgacc
     4441 gacctttacg cgaccggcgt catgctgttc gagatgctga ccggccggct gccattcact
     4501 gccgagaacc cggtcgcgat gatgatggcg caccaggatc agtcgccgcc ccgaccgagc
     4561 caaatcgcgc ctggcaacgc catctcaccg gccgtcgagg gcgcagtgat gcgggtgctg
     4621 gccaagcggc ccgcggaccg cttgcagagt gcgagctcgt tcctcgacgc actgtccggt
     4681 cgagccgcgg cgccgacgac tcgtggcgcc ccacctaccg ccacgatgcc gatacagcgc
     4741 gcaccacgca tacagccggc ggcggtccct cagcgtccgg tccgacaacc agccccggta
     4801 cggcgatcat ccgcgtggag ctggctgctg gttgtgctcg tgatcgcggg agtcgctgcc
     4861 gccggcgcgg cgtatgccac cggttacttc gattcgtcag acgctccgaa tcagaacgca
     4921 ccggtcgttg agccgaccgc caccgacact ccgatcgacg agccaacgca atcggcgatc
     4981 gaaggtccga cgatagcgcc tgtagaggag gcgaccgaac cagtcgaaac gccgacgccg
     5041 gtattcgccg aggagccgac cgacacgccg gaaagtagcc cgcacgtgcc aatcgaaccg
     5101 atcaacggcg ccggaccata atcctgttct agtcgagaat ggctgaagga cgcctgccca
     5161 ggatcgattc ggcgtcggcg gttatgcgcc gaacaagctc tccggccggt tcgatcgagt
     5221 ggatcacctc ggacgcgctc ccggcccagg ccgcgctcca ctcggcggtg ccggaagata
     5281 tggcagccgc gaattccgca tcgagctcct cgcgccggga gacgatctcg tcgtcgcgcc
     5341 catgccattg ctcggtgaac cggttcttga tcgcccgccc cgcaatgccc tcggggaacg
     5401 ggacgttgcg ggcgaggtcg tacgcgagcg tgcgcaccgt gtcgtccgcg ccggccgcaa
     5461 cgatcgcgtc cttgtgccac ttcccggagg cggattcggt gctggcaacg aagcgggtgc
     5521 ccatccaggc gccgtccgcg cccatcatga tggctgccgc caatccacgc ccgtcggcga
     5581 ttccaccggc ggccagcacc ggcacgtcga ccgcatcgcg cacagccggg aggagcgtga
     5641 aggtgctgat ttcaccgatg tggcctccgc cctcgactcc ctgcgcggcc aggacatcaa
     5701 cgccggccac agccagccgc ttcgcctccg cgacagtctg gacctggctg agcagcaccg
     5761 cgccggccgc cttcacgcgc tcaacgtacg ggataacgtc gccgaaggag atcgccaccc
     5821 cggcaacccc ttcggcgagc gaaacggcca ggagctcgtc gaattgcggg aagcagagcg
     5881 ccaggccgac cccaaacggc ctgtcggttc gctcccggat ctgccggatt tgcgctcgga
     5941 gccactcggg ccgttccggc ttggccgtgg cgtgcgacgc gccgatcaag ccgaacccgc
     6001 ccgcggccga gactgccgcc acgagctccg cataggcgct gccggccatt ggcgctgaga
     6061 cgatcggatg cgtgatgccg agtaattccg taaaccgggt ccgcactatc tctcctccgc
     6121 tcgtattcgt cgagcagcga ccaagccgtt tgcgtgctgc caggccaggc cggcaattgc
     6181 gccgatcccg atcgcgatcg ccggtaacag cgggagcatg tatcgcgcgt aatcgagccg
     6241 gaacgtcgcc gcgatcaggg ccacctgtcc gcccagaacc agcagcggca ttacctgatc
     6301 agccgggaac ctcctccgaa ccagaaggac gaagagcagg atccagccgg ccgccgccag
     6361 catgagatcc agctcgcgga accagagcca gtccccgacg tcgacacgtc tccggagctc
     6421 gctgagcaga tatccggccg tgctctcgcg ctcgccaagc tgaacgccga accggcgcgt
     6481 tgcgtcgccc aggccgatca cccgcgcgtg gggcgagacg tcagcctgcc agtcgaagct
     6541 cagctccctg aactcgaaca tgcgctgagc gtgcgtgacc ggatcagtcc agaggtaggg
     6601 gtacaacgcg aagaacgtca ggccggccac gatcgggatc aacccgatcg ccagaccgga
     6661 ccgtagtgcc accctccgcc gcgtggccac gccccacgcg atcggtgcaa tgcccagcgc
     6721 gccgatcgcc gccgccagca ggagcggact cagctttgtc gccgccccaa gcccgagcag
     6781 gacgccgatc aacgcagccc gcccgaaggt cggccgcgcg ccgaaccgga tcgcggcccc
     6841 ggcggcggcc acgatgcaga ggactacaag cggatcagag aacgcgcggg tcgacgtctc
     6901 ggtcaccagg ggatgcagtg cataggccag cccggcaatt acgccgccga gccgattcgt
     6961 cagccgcgcg ccgatcgcgt acaggatcag gacagtcagc agcgcaacga cgacgttcgt
     7021 tcgccgtgcg gcccggagct ccgagccgga cggcatgttg ccgtgcgccg tgttccactc
     7081 cgtatccttg tccatgatcc agtagccggt ttgtggcagc ccctcgccct gcacggtcaa
     7141 cccgatgccg aagagataca tggcgagcgg cggctgagac cgtcgccggt tgacctcatc
     7201 gatcgtgccg tattcgttgc ggtacccctc gtcctgccat ttctcgccga gcggatgtcg
     7261 ccactcgcgc agcacggccg agttcccgat ccaacgcgcc tcgtcacggt gaaagggagt
     7321 cgtcgcgacg gcaggccaca ggtgccagag cgtgacagcg ctcaacagcg cgatcgccgc
     7381 gaatgttcgc caccttgccg caatcgggat agcgcgccgc tggacacggg gttgggattc
     7441 aacgggaatg atgagggtcg gcatccagca ggctcactgg gtcagggttg cgggcagacg
     7501 gtgctgccat gggattgata gcacgaattc gtcgccgcgc tcggcactga ccgggatttc
     7561 gcccgtacac tgctttccgc cgcgcgtttt gcgccggctc ggcggtcgat cgcgcatcgc
     7621 cgaagtccag atgacgaccc ggagagggga gccaccttgg attggatatc gaaccccgag
     7681 atctggaccg cgctgctgac gttgaccgcg ctcgagatcg tgctcggcgt cgacaacatc
     7741 atcttcatct cgatcctggc cggcaagttg ccggtgcacc agcgggatcg cgcccgtatc
     7801 ctcggtctcg cggccgcaat gctcacccgc atcctgctcc tcttcacctt gaactggatc
     7861 atcggcctga ccaggacgct cttcacggtc tttggagaag atatctccgg gcgtgagctg
     7921 attctgctgt ttggcggcct gttcctgctt ggaaagagca cattcgagat ccacgagaag
     7981 ctcgaggggg cggaggagca tagtgcccag cgaacgggtg catcgtttgc atcggtcatt
     8041 atccagatca tgctgctcga catcgtcttt tcgcttgact cggtgattac tgcggtcggc
     8101 atggccaagc acatcgaagt gatggtcgcg gccgtggtga tctcagtagg gatcatgctc
     8161 atcgcctcgg gtccgatcag ccggttcgtc gatcagcatc caaccgtcaa gatccttgcc
     8221 ctgagcttcc tgatgttgat cggcatgtcg ctcgtggccg aggcgttcga gcagcacgtc
     8281 ccgaagggct acatctattt cgcgatgggc ttctcggtcc tcgttgaagt gctgaacctg
     8341 cgaatccgga aaaaggatgt cgagccggtt cagctccaca acccccgcat gaacgaagaa
     8401 atcgtcgacg cggctgctcg cgtcggc
//
LOCUS       DASSGX010000328         7095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_820287, whole genome shotgun sequence.
ACCESSION   DASSGX010000328 DASSGX010000000
VERSION     DASSGX010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7095
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820287"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="VFI12_05800"
     CDS             <1..576
                     /locus_tag="VFI12_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055956.1"
                     /translation="DPQADGSYLARISDKVLQEYVDFCEKRKLHLLLDMQYGRLTTEQ
                     ELEAIAPWLEYPFVHPALDPEFSIEEGEVPGENLGSIDGSDVTFAQEWVADFCATRGL
                     PPKILIVHQFNVYSISNKETIHPVDGVQFCLEVDGFGTPDMKLETYGVLTRDPIEFHG
                     FKLWYSGEDDPLMSETDVVNLDPSPDVIIYQ"
     gene            704..1330
                     /locus_tag="VFI12_05805"
     CDS             704..1330
                     /locus_tag="VFI12_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055957.1"
                     /translation="MARNLRHRSFGACARTGFVLFIATLLLSGAAGFALRPARAADTP
                     AVALSAGCTGLNDPFYDGRSAGANAGTGSFLAGERISIAAGDPSSGAPQSFTVYVNFM
                     DTVIRAFPGTFTFTVPTDGSYNIFWGIDTGTDATWSVSCREPGQDSTPTPTPEPTATA
                     VPSTDTCVARGNENAERASQNGKEHSCLDQSKTSTLSNKPGKGNKHNR"
     gene            1444..1677
                     /locus_tag="VFI12_05810"
     CDS             1444..1677
                     /locus_tag="VFI12_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055958.1"
                     /translation="MSSDPNETPADPIDYDAIVEHRLAAITSRFPDRFTGEQVDQIRR
                     RVARSVTLGANLAKVELPNGIGPNFDPRAMANG"
     gene            1670..3061
                     /locus_tag="VFI12_05815"
     CDS             1670..3061
                     /locus_tag="VFI12_05815"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET7055959.1"
                     /translation="MADDLSFASLVELGLRLRAGELTSVQLTEHALDRLERHGPALNA
                     VVTLTRERALREARQADEERAEGIDRGPLHGIPYGAKDLLATIDYPTTWGAEPFRNQQ
                     FDHDADVITRLREAGAVLVAKLSMVELAGGLGYEQPNAALTGPGRSGWNPDRWAGGSS
                     SGSGSAVAAGLVPFAIGTETWGSIHCPSSFNGITGLRPTYGRVSRGGAMALSWTMDKI
                     GPMARSAADCALVLRAIAGASATDSTTLGQPELIADDRRGGFRFGLLKGSIESSAPGV
                     AANATGAVERLREIGTVEEIELPELPWDDAASIIIMCEAASAFADFLARGDGMRLTAP
                     EDRTGLYHALTVPSTDYLQAMRIRTFGAGRMAELLAGFDALVAPAYPVTPPPAVGNFE
                     AYFDKHPGHSLSAMGNLVGLPSIAVPTGLDDGLPTSLEILTSWWTESTAVAIASAYQS
                     KTTWHTAKPPLFA"
     gene            complement(3337..4170)
                     /locus_tag="VFI12_05820"
     CDS             complement(3337..4170)
                     /locus_tag="VFI12_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055960.1"
                     /translation="MTVWLCATCGLEHADTERPPESCAICSDDRQYIPRTGQRWTTLP
                     EVQVDRHAQISEMEPDLYGIAITPKVGIGHRPLLIRTPNGNLLWDPPGFVDDVLVTEI
                     KRLGGIAAIASSHPHLTGVSVTISHLFGSVPVWYNADDQRWIRRPDEVIRLWRDLQDV
                     LPGLTVVQCGGHFPGSAVLHWPAGANGRGAIMTGDTIRPNADWHTVSFMRSYPNLIPL
                     PPRSVQKIAAAVAPLAFDRIYGGFEGELIDTNGADAVQSSARRYMEWVTDQVQDLDER
                     V"
     gene            4388..>7095
                     /locus_tag="VFI12_05825"
     CDS             4388..>7095
                     /locus_tag="VFI12_05825"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET7055961.1"
                     /translation="MPVVPDPANVETKLPEPPERIGAIDPELQAERAAVEVARVLEAE
                     LTARISGEVRFDRTSRMLYSTDASNYQIEPVGVVIPKTADDAIGAVELAASHGVPILP
                     RGGGSSLAGQTVGAALVIDFSKYCNRVLDLNLEAGTVSVETGSSLDLLNRQLKANGLM
                     FGPDPSSSNRATIGGVIGNNSTGAHSILYGMTADNVVSTRITLSGGGTFELGPGSPEE
                     LTALAAHSDARGRLLTQLLDYQERHSDLIARDFPPHWRRSTGYSLNEFIKSDFNPGRL
                     MASSEGTLATVLSATMKVVPVPRKTALVILQFADLVASMEVTPLILETGPSAIELMDR
                     MLIDLTRAQPAYAPQIAFIEGDPAAVLCVEFYGETEQELQQKCDHLLARLTGKSIGMS
                     ADPIVVMEPKQQADIWKVRKDGLGLLYSIRGDFKPIPVIEDVSVPVEHLPEFVRTVEE
                     MVAEYETTAAYYAHASAGCLHIRPLVNLKEQRGVEIMTELADRATEMARRFGGVMSGE
                     HGDGLQRSELNERIFGPELYEAMRELKRIFDPAGLMNPGKKVDAPSMIENLRFGPSYN
                     PAPINTYFDYTREGGFLRAVEQCNGSAVCRKLKAGTMCPSFMATRDEQDTTRARANAL
                     RNALAGRAGFELKDFTSQATYDVMDLCLSCKACKTECPSSVDMAKLKMEFLAHYQDKH
                     GTPLRSRVFGHIHTISRLTRPVAPIANFVLSRRLSRPLMRAMGVHPDRKLSPFQRATF
                     SSWWRKHAANLPFGRKTRGKVVYFHDTFTEYNYPRIGVAAVQLLEACGFEVVIEHRRA
                     CCGRPMLSKGLLNDARRMAARNVALLAPYANAGIPIIGTEPSCILTLRDEYIDLLPMN
                     GDAEAVAGHSYMIDEFLAKLETTGRLGVGWKSDPQPVMFHGHCHQKA"
BASE COUNT         1225 a         2390 c         2301 g         1179 t
ORIGIN      
        1 gatccgcagg ccgacggaag ctacctggcc cggatctcgg acaaggtgtt gcaggagtac
       61 gttgatttct gcgaaaagcg gaagctgcac ctgctgctgg acatgcagta cggccggctg
      121 acgaccgagc aggagttgga agcgatcgcg ccctggctcg agtacccctt cgttcacccg
      181 gcgctggacc ccgaattctc aattgaagag ggtgaggtgc cgggcgagaa cctcggctcg
      241 atcgatgggt ccgatgtcac cttcgcccag gagtgggtgg ccgacttctg cgcgacccgc
      301 gggctgccgc ccaagatcct gatcgtccac cagttcaacg tctattcgat ctcgaacaag
      361 gagacgatcc atccggtcga cggcgtgcag ttctgcctcg aggtggacgg attcggcacg
      421 cccgacatga agctggagac gtacggcgtc ctcacccgcg atccgatcga gttccacggc
      481 ttcaagctct ggtacagcgg cgaggacgac ccgttgatgt cggagacgga cgtcgtcaac
      541 ctcgacccat cccccgatgt gatcatctac cagtagttac ctcccgacca aactcagcac
      601 cttgccggcc agggatcgac tgtattccgg accggttcgc tggtaggctg gtgccactgc
      661 cccatgaaca aaccgtgtgc attggtatga ggaggagagt gcaatggcac gcaacctgcg
      721 acaccgctcg ttcggtgctt gcgctcggac ggggttcgtc ctgtttatcg cgacactgct
      781 gttgtccggc gcggccggtt tcgccctgag accggcgcgc gccgccgaca caccggcagt
      841 ggcgctttca gccggatgca caggcctgaa cgacccgttc tatgacgggc gctccgccgg
      901 cgcgaatgcc gggaccggca gcttcctggc cggcgagcgt atttcgattg ccgccggtga
      961 cccgtcttcc ggcgcgccgc aatcatttac tgtctatgtg aacttcatgg acacggtcat
     1021 cagggcgttc cccggcacct tcactttcac cgttccaacg gacggcagct acaacatctt
     1081 ctggggtatc gataccggta ccgacgcgac ctggtcggtg agctgccggg agccgggtca
     1141 ggattcgacg ccgaccccga caccggaacc gacggcgact gccgtcccgt ccactgacac
     1201 ctgcgtggcg cgcggcaacg agaacgcgga gcgcgcgagc cagaacggca aggagcacag
     1261 ctgcctcgac cagtccaaga ccagcacgct gtcgaacaag ccgggcaagg gcaacaaaca
     1321 caaccgctaa ccagtgcgtt tcgtgacgag acgggtgccc gatttcccgg gcacccgttt
     1381 tgcgtgccct gcccgatcct gtactctgcc gggaacgtgg tcgcctgcct gacgggagaa
     1441 tgcatgagct ccgatcccaa cgaaacgccc gctgatccga tcgactacga tgccatcgtc
     1501 gagcatcgct tggcggcgat cacgagccgc ttccccgacc gatttaccgg cgagcaggtc
     1561 gaccagatcc ggcgccgggt cgcccgcagc gtgacgcttg gcgcgaacct cgcgaaggtc
     1621 gagctgccaa acggtatcgg gccgaatttc gacccgcgag cgatggccaa tggctgacga
     1681 tctctccttc gcctcgctgg tcgagcttgg tctccggctc cgggccggtg agctcacgtc
     1741 ggtccagttg actgagcacg cgctcgaccg gctagagcgg catgggccgg cgctgaacgc
     1801 cgtcgtcacg ctcacccgcg aacgcgccct tcgcgaggcg cggcaggctg acgaggagcg
     1861 tgccgaaggg atcgatcgcg gccccctcca cggcatcccc tacggcgcca aggacctgct
     1921 ggccacgatc gactacccga cgacctgggg cgcggagccc ttccggaacc agcagttcga
     1981 ccacgatgcc gacgtgatca cccggctgcg agaggcaggc gcggtgctgg tcgccaagct
     2041 ctcgatggtc gagctggccg gcgggctcgg ctacgagcag ccgaatgccg ccctgaccgg
     2101 tcccggccgg agcggctgga acccggatcg ctgggccggt ggatcgtcga gcggttcggg
     2161 ctccgcggtc gcggccggat tggtcccgtt tgcgatcggg accgagacgt gggggtcgat
     2221 ccactgcccg tcgtcgttca acggcatcac cggcctgcgc ccgacatatg gccgggtgag
     2281 ccggggcggg gcaatggcgc tctcctggac gatggataag atcggcccga tggcccgttc
     2341 cgccgccgat tgcgcgctcg tcctgcgggc gatcgccggc gcgtcggcga cggacagcac
     2401 gacactaggc cagccggagt tgattgcgga cgatcgccgg ggcggattcc ggtttgggct
     2461 gctgaaaggg tcgatcgagt cgagcgcgcc gggcgtcgcc gcgaacgcga cgggggccgt
     2521 cgagcggttg cgcgagatcg ggactgtcga ggagatcgaa ctgcccgaac tcccgtggga
     2581 cgatgcggcg tcgatcatca tcatgtgcga ggcagcctcg gccttcgcgg atttcctggc
     2641 gcgtggcgac gggatgcggc tgacggcgcc cgaggatcgc accggtctct accacgcctt
     2701 gaccgtgcct tcgaccgact atttgcaggc gatgcggatc cggacgttcg gcgcgggccg
     2761 gatggcggag ctgctggccg gattcgatgc actggtggcg ccggcctacc cggtcacgcc
     2821 gccgccggcc gtgggcaact tcgaggcgta tttcgacaag cacccgggcc acagcctgag
     2881 cgcgatgggc aacctggtcg gcctgccgtc gatcgccgtc ccgaccggcc tggatgacgg
     2941 cctgccgaca agcctcgaaa tcctgacctc gtggtggacg gaatcaacag cggtcgcaat
     3001 cgcctccgcc taccaatcga aaaccacctg gcacaccgca aagccaccac tgttcgcgta
     3061 atcagtgtcg ccgggggaat gggcactgtc ggggtccgcg ggggcgggcc cgccgctcca
     3121 ccagggcccg gcggccaagg gcggaccggc ggatgaacgc catgccaaac gggaaaggcg
     3181 ttcgcggtca acgcgcgggc gtgcccccgc acgcccctac accggtacga gtgtcgcccg
     3241 gcgggccagc ggaggcagag gcgcatcgca tatgcccgca catccgatgc cgaccagccc
     3301 gatccctggt ccctgactca cgactcacga ctcgcctcac acccgctcgt ccaggtcctg
     3361 cacctggtcc gtgacccact ccatgtagcg gcgggcagac gactggacgg cgtccgcgcc
     3421 gttggtatcg atcaactcgc cctcgaaccc gccatagatg cggtcgaacg cgagcggagc
     3481 gaccgcagcg gcgatcttct ggactgagcg aggcggaagc gggatcaggt ttggatagct
     3541 ccgcatgaac gagaccgtgt gccagtcggc gttggggcgg atcgtgtcac cggtcatgat
     3601 cgcgccgcgt ccgttcgcgc cggcgggcca gtggagcacg gcgctgcccg ggaagtgtcc
     3661 gccgcactgg acaaccgtca ggcccggcag cacgtcttga aggtcgcgcc aaagccggat
     3721 gacctcgtcc ggccggcgaa tccagcgctg gtcgtccgcg ttgtaccaga ccggcacgct
     3781 accgaagaga tgactgatcg tgaccgagac gccggtgagg tgcgggtggc tggaggcgat
     3841 cgccgcgatc ccgccgagcc gcttgatctc ggtgaccagc acgtcgtcga cgaatcccgg
     3901 cgggtcccag agcaggttgc cgtttggcgt ccggatcaga agcgggcgat ggccgattcc
     3961 gaccttcggc gtgatcgcga tgccatagag gtccggctcc atctcgctga tctgcgcgtg
     4021 gcgatcaacc tggacctccg gcagcgtcgt ccagcgctgc ccggtgcgcg ggatgtactg
     4081 tcggtcgtcc gaacagatcg cgcacgactc cggcggccgt tccgtatccg catgctccag
     4141 cccacacgtc gcgcataacc aaaccgtcat cggtcatctc ctggcgtcta tcaattccag
     4201 caacgcattt taggttttgg gtttcaggtt ccaggttttg gactctttgt tccctcaact
     4261 ctcaattctc aagcctccac cctcaacctg acttcgcgcc tcagcgcctt cgcgcctccg
     4321 cgcctacaat tcccccacga caatcaccga gcgccacccg gcgttgcgta gagagagacg
     4381 gcgcctcatg ccggttgtac cagaccccgc gaatgtcgag acgaagttgc cggagccgcc
     4441 cgagcggatc ggcgcgatcg atcccgaact gcaagctgaa cgtgctgccg tcgaggttgc
     4501 gcgtgttctc gaggctgagc tgactgcccg aatcagcggc gaggttcgct tcgatcgcac
     4561 ctcgcggatg ctctattcga ccgacgcctc caactaccag atcgagccgg tcggcgtggt
     4621 catcccgaaa acagccgatg acgcgatcgg cgctgtcgag ctggccgcga gccacggcgt
     4681 gccgatcctg ccgcgcggcg gtggctcgtc gctggccggg cagacggtcg gcgcggcact
     4741 cgtcatcgac ttctcgaagt attgcaaccg cgtgctcgac ctcaacctcg aagccggtac
     4801 ggtctcagtc gagaccggct cctcgctcga cttgcttaac cgccagctca aggcgaacgg
     4861 gttgatgttc gggccggatc cctcatccag caaccgggcc acgatcggcg gcgtgatcgg
     4921 caacaactcg accggcgcgc actcgatcct ctacgggatg accgccgaca acgtggtcag
     4981 cacgcgcatc accctttccg gaggcgggac attcgaactc ggacccggct cgcccgagga
     5041 actgaccgcc ttggcggccc atagcgacgc ccggggccgc ctcctgaccc agttgctcga
     5101 ttaccaggag cgccactccg acctgatcgc gcgcgatttc ccgccgcact ggcgccgctc
     5161 gactggctac tcgctcaacg agttcatcaa atccgatttc aatcccggcc ggctgatggc
     5221 ctcgtctgaa ggcacgctgg cgacggtcct ttcggccacg atgaaggtcg tgccggtgcc
     5281 gcgcaagacg gcactggtca ttctccagtt cgccgacctc gtcgcgtcga tggaggtgac
     5341 cccgctcatc ctcgagaccg gcccatcggc gatcgagctg atggacagga tgctgatcga
     5401 cctgacccgg gcgcagccgg cctacgcgcc gcagatcgcc ttcatcgagg gcgacccggc
     5461 ggcggtcctt tgcgtcgagt tctacggtga aaccgaacag gagctgcagc agaagtgcga
     5521 ccacctgctc gcccggttga ccggcaaatc gatcgggatg agcgcggacc cgatcgtcgt
     5581 gatggagccg aagcaacagg ccgatatctg gaaggtgcgc aaggacgggc tcggcctgct
     5641 ctacagcatc cgcggcgact tcaaaccgat cccggtgatc gaggacgtct cggtcccggt
     5701 cgagcatttg cccgagttcg tgcggaccgt cgaggagatg gtcgccgagt acgagacgac
     5761 cgctgcgtac tacgcgcacg catcggccgg ttgcctgcac atccgcccgc tggtcaacct
     5821 gaaggagcag cgcggcgtcg agatcatgac ggaactggcg gaccgcgcga ccgagatggc
     5881 gcgacggttc ggcggcgtga tgagcggtga gcacggcgac gggctccagc ggtcggaatt
     5941 gaacgagcgg atattcgggc ccgagctgta cgaggcgatg cgcgagctga agcgcatctt
     6001 cgacccggcc gggctgatga acccgggcaa gaaggtcgac gcgccctcga tgatcgagaa
     6061 cctgcgcttc ggcccgtcct acaaccccgc tcccattaac acctacttcg actacacgcg
     6121 ggagggcggc ttcctgcgcg cggtcgagca gtgcaacggc tcggcagttt gccggaagct
     6181 caaggcaggg acgatgtgcc cgtccttcat ggccacgcgt gacgagcagg acacgacccg
     6241 tgcccgcgct aacgcgctcc gcaatgcgct ggccgggcgg gccggattcg agctgaagga
     6301 cttcacctcg caggcaacct acgacgtgat ggacctctgc ctctcctgca aggcgtgcaa
     6361 aaccgaatgc ccctccagcg tcgacatggc caagctcaag atggagtttc tggcccacta
     6421 ccaggacaag cacggcacgc cgctgcgatc gcgcgtcttc ggccatatcc acacgatctc
     6481 gcggctgacc cgaccggtcg cgccgattgc caacttcgtt ctctcccggc gattgagccg
     6541 gccgctgatg cgggcgatgg gtgtccatcc cgatcgcaag ctgtcgccgt tccagcgcgc
     6601 taccttctcg agttggtggc ggaagcacgc ggccaacctg ccgttcggcc ggaagacccg
     6661 gggcaaggtc gtctacttcc acgacacctt caccgagtac aactacccgc ggatcggcgt
     6721 cgcggccgtg caattgctgg aggcgtgcgg gttcgaggtg gtgatcgagc atcgccgcgc
     6781 gtgctgcggc cggccgatgc tctcgaaggg gctgctcaat gacgcccggc gcatggcggc
     6841 gcgcaacgtc gcgctgctcg cgccgtacgc gaacgcggga atcccgatca tcggcaccga
     6901 gccgagctgc atcctgactc tgcgggatga gtacatcgat ctcttgccga tgaacggcga
     6961 cgccgaggcg gttgccggcc actcctacat gatcgacgag ttcctggcca agctggagac
     7021 gaccggccgg ctcggcgtcg gttggaagag cgacccgcag ccggtcatgt tccatggaca
     7081 ctgccaccag aaggc
//
LOCUS       DASSGX010000329         2764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1365430, whole genome shotgun sequence.
ACCESSION   DASSGX010000329 DASSGX010000000
VERSION     DASSGX010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1365430"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VFI12_05830"
     CDS             complement(<1..194)
                     /locus_tag="VFI12_05830"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase / HUMPS
                     family protein"
                     /protein_id="HET7055962.1"
                     /translation="MTRLQLAIDLVAPDDFERVVVPLLPLIDVVEAGTPLIKQNGMAA
                     VQALRLLAPNHLLVADMKTM"
     gene            complement(191..1381)
                     /locus_tag="VFI12_05835"
     CDS             complement(191..1381)
                     /locus_tag="VFI12_05835"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7055963.1"
                     /translation="MVDSRPYRRDTITLFLLATTAMHRSVFHSMGAVMPALRKDLGIS
                     ATLTNAHVSAIALGMMAMGLVADRVAARVGRRPAVWLGMAGIVLGMVLLALAPVVWLT
                     LPIALLFIGALGSLVLALTPAALSDHQGEHRAVALSESSLVSSIIGGIVPATVGLSQR
                     LGFGWGGATIVPVAILGAMAAKFRSTPVPDTVPHPDQPQSTQPLTRGFWAFWLTLVFT
                     DSFEFGLIFGAAVLLEFRGMSQAGAATALSLLFWGMIVGRFAGTRLVRRVSPLRLLRF
                     TFALAAIGTVAHWLSPVLILSIIGLFLAGIGISNLYPLTTSIGLQAAEFNSSAAGSRL
                     SLASGISMLVAPLLFGVLTDLADIRVAFAVEPIFLLLAVGAAAYGARWLTRPAPVSVE
                     AFAT"
     gene            complement(1389..>2764)
                     /locus_tag="VFI12_05840"
     CDS             complement(1389..>2764)
                     /locus_tag="VFI12_05840"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET7055964.1"
                     /translation="AHERGLKVIIDFVPNHTSDQHPWFIESRSSRNNPKRDWYIWRDP
                     KPDGSPPNNWLATFGGPAWTLDEATGQYYHHGFLPQQPDLNWRNDEVKDAMLDNIRFW
                     LERGVDGFRFDVAHHIMKDPELRDNPMNEGVTALHKPMGEFGTQLHVMDRMHSDLHGM
                     YRQIRSVINSFDTELQPRFMIGETHVFDPTTWATLYGENLDEMHMPANFGLLKVGWNA
                     KEVRGHVDAIEAALPPGAWPNYVLSNHDDWRTATRVGQKQVKVAMMLLLSLRGTPTVY
                     YGEEIGMENVEIPPHLERDPAGLRVPGIGLGRDPERTPMQWDASPNAGFCPDGVMPWL
                     PIADDYKTRNVAVKKEDPKSILNLTRELLELRKSHPALAVGDYAPIDGLPEQCFVYTR
                     STSDQCLLIALNLTNEEHQFMLPYTGTGQVVTSTGKDRTGKISLTFMVLQPNEGILID
                     ITGAKLA"
BASE COUNT          479 a          861 c          974 g          450 t
ORIGIN      
        1 tccatcgtct tcatgtcggc gaccagcagg tggttgggcg caagcaatcg cagtgcctgc
       61 accgccgcca tgccgttctg cttgatcagc ggcgtgccgg cctcgacgac gtcgatcagc
      121 ggcaggagcg ggactacgac gcgctcgaaa tcatccggtg cgaccaggtc gatcgccagt
      181 tgcagcctgg tcacgtcgcg aacgcctcga ccgagaccgg cgccggacgg gtcagccacc
      241 gcgccccgta ggctgccgcg ccgacggcca gcagcagaaa gatcggttcg accgcgaagg
      301 cgacccggat atcggccagg tcggtcagca cgccgaacag cagcggtgcg accagcattg
      361 agatgccgct ggcgagcgag aggcgcgaac cggccgcgct cgagttgaac tcggccgcct
      421 ggagcccgat cgaggtggtc aggggataga gattcgagat gccgataccc gccaggaaca
      481 atccgatgat cgaaaggatc agcaccgggc tcagccagtg cgcgaccgtg ccgatcgccg
      541 cgagcgcaaa cgtgaagcgt agcagccgga gcggggagac gcggcggacc aatctggttc
      601 cggcgaaccg cccgacgatc atgccccaga acaacagcga gagggcggtc gccgcgccgg
      661 cctgggacat gccacgaaat tcgagcagga ccgccgcgcc gaagatcagg ccgaactcga
      721 acgagtcggt gaagacaagt gtgagccaga aggcccagaa tccccgggtc agcggctgcg
      781 tcgattgcgg ctgatcgggg tgtgggaccg tgtcgggcac cggcgtggac cggaacttgg
      841 cggccatcgc gccgaggatc gcgaccggca cgatggtcgc gccgccccag ccgaagccga
      901 gccgctggct gagaccgacc gtggccggga cgatgccgcc gatgatgctt gagaccagcg
      961 acgactcgga gagggcaacc gcgcggtgtt ctccctggtg gtcggagagg gcggccggtg
     1021 tcagggcgag gacgagcgac ccgagcgcac cgatgaagag cagcgcgatc ggcagcgtca
     1081 gccagacgac cggcgcgagg gcgagcagca ccatcccgag cacgatcccg gccatgccga
     1141 gccaaaccgc cggccgccgg ccgacccggg ccgcgacccg atcggccacc agccccatcg
     1201 ccatcatgcc gagcgcgatc gcgctgacat gcgcgttggt gagtgtggcc gagatgccca
     1261 ggtccttgcg cagcgccggc atgaccgctc ccattgaatg gaagacggag cggtgcatgg
     1321 ccgtcgtggc cagcaggaag agggtgatcg tgtcccggcg atagggacgg ctgtcgacca
     1381 tcgccggatc aggcgagttt ggcgccggta atgtcgatca ggataccctc gtttggctga
     1441 aggaccataa aggtcaggct gatcttgccc gttcggtcct tgccggtcga cgtcacgacc
     1501 tggccagtgc cggtataggg cagcatgaac tggtgctcct cgttcgtgag gttgagcgcg
     1561 atcaacaggc actggtcgct cgtgctgcgc gtatagacga agcactgctc gggcaggccg
     1621 tcgatcggtg cgtaatcgcc gaccgccagc gccgggtgcg attttcgcag ctcgaggagc
     1681 tcgcgggtga ggttgaggat cgacttcgga tcctccttct tgaccgcgac gttccgggtc
     1741 ttgtagtcgt ccgcgatcgg caaccagggc atgacgccgt ccgggcagaa gccggcgttc
     1801 ggcgacgcat cccactgcat gggagttcgc tcgggatcgc gaccgaggcc gatgccgggc
     1861 acgcgcaggc cggccgggtc ccgttccagg tgcggcggga tctcgacatt ctccatgccg
     1921 atctcttcgc cgtagtagac cgtcggcgtg ccccgcaacg agaggaggag catcatcgcg
     1981 accttgacct gtttctggcc gacgcgcgtc gcggtccgcc agtcgtcgtg gttgctgagc
     2041 acgtaattcg gccaggcgcc gggcggcagg gcggcttcga tcgcgtcgac gtgaccgcgc
     2101 acttccttcg cgttccagcc gaccttgagc aggccgaagt tggccggcat gtgcatctcg
     2161 tcgaggtttt cgccgtagag cgtggcccac gtagtcgggt cgaagacatg cgtctcgccg
     2221 atcatgaagc gcggctgcaa ctcggtgtcg aaggagttga ttacggaccg gatctggcga
     2281 tacatgccgt gaaggtcgga gtgcatccgg tccatgacat ggagctgcgt gccgaattcg
     2341 cccatcggct tgtgcagggc ggtcacgccc tcgttcatcg ggttatcgcg gagctccggg
     2401 tctttcatga tgtggtgcgc gacatcgaac cggaagccgt ccaccccgcg ctcgagccag
     2461 aagcggatgt tgtccagcat cgcgtccttg acctcgtcgt tgcgccagtt gaggtcgggc
     2521 tgctggggga ggaagccgtg gtggtagtac tggcccgtcg cctcgtcgag cgtccaggcc
     2581 ggaccaccaa aggttgccag ccagttgttc ggcggcgatc cgtccggctt cgggtcccgc
     2641 cagatgtacc agtcccgctt cgggttgttg cggctggagc gggactcgat gaaccaggga
     2701 tgctggtcgg aggtgtggtt tgggacgaag tcgatgatga ccttgagccc gcgttcgtgg
     2761 gctt
//
LOCUS       DASSGX010000330         2788 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1583963, whole genome shotgun sequence.
ACCESSION   DASSGX010000330 DASSGX010000000
VERSION     DASSGX010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1583963"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(480..>2788)
                     /locus_tag="VFI12_05845"
     CDS             complement(480..>2788)
                     /locus_tag="VFI12_05845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET7055965.1"
                     /translation="GRYFLDTNLAGWKVRRRNVLAILMTLYWLPILFVAAFANENNER
                     YLLHLHPLGLILIGFAAQELIVGERVRPALSFAPAAGTMALPGSRIEVVGERFAPTLQ
                     WLTPARVYAAAVAFAIGVGAVLRVWGYNRLSMWLDEGFSLLYSKQDWAAATGFHGFYS
                     PHPPLYFTLIKVFNLVLPDVSAGRTVAVLCGVLVLPVFYLLARRLLDPVAALVATTVF
                     MLSPIHIYYSQEARMYSLVVLSVTASFLALVGFIQTLDRRWAALYFLSLAVAVYADYS
                     SLFVLGPQAGVLLYYVWRYRGKMIPLLIAAGLAVLAYVPWLPQVWNSVNSANEDERRA
                     DYLGAGFKRIMLIVLRITGVSSDNRGAYFPSLAKTPWDRLHDLQSLILIAMAPVVILG
                     VVGLWQRWTAMAVVFSFLGCIVVAVIVSLLSPGFAERTILSAAVGWSLLLGAAFNGRI
                     NRQRTPLAAASLLVVLVLCLGTIGNIHASAVKQRWDDASADLAMVSPLEFPVVTYSYG
                     AVADTLVEAYEPGLLDSMRLITVRDGELEKTLSNDTIPQKGITIADVDAGKLSELLPD
                     TPDNDVIWYLYYQRRGEEFVRAGIERAGYVRIIDKVYDAPRGLVFLDLYARPGAALGD
                     PVPGVATYQNAAAWGIPAGRSPVQSSEDGTSVAITNQSRAGTAVVTQVKTSGRALYTL
                     DVDVQSRLLGSSALVTLTCQTQAGGVLNALTAGTQGAKVNDVMHHQSAIYCPEETAQV
                     RITLTNLGNGEMIFMTPTLRKLAIPDRE"
BASE COUNT          512 a          900 c          943 g          433 t
ORIGIN      
        1 cgagcagtgg gacattgtgc ggggttacca cgccctgata tcgcccgcac ggcgatgact
       61 gtcggtggac ggtgggttcc ggacacccaa ccggcttacc ggcgccgccc ggagctaaag
      121 gtccgggcta aatgacaaga agtccactaa agggactaga aggaagtcgg cttcagccgc
      181 cttattctct ctcagcgccg gctttcagcc cggggcggtg acctgggcgc ggtcgccatg
      241 gagcggatct ccgggcagca cagccgggcg gggccgcccg gggcgccatt gtcgagagag
      301 cgcctgtccc agccccttca gtgggcttct gcctgttcag cccgggcctt tagccccggg
      361 cggaggttga cggccggcta ggtacttgga acccgccgtc cactaacgat catcgcccgg
      421 cgggcgattg cgaggcgtgg taactcctcg ccaggatgaa gggtacgccg gttcgttcgc
      481 tattcccggt cggggatggc gagtttgcgc agggtgggcg tcatgaagat catctcgccg
      541 ttgccgaggt tggtcagggt gattcgcacc tgtgccgtct cttccgggca gtagatggcg
      601 gattggtggt gcatgacgtc gttcaccttg gcgccctggg tgccggccgt cagtgcgttc
      661 aggacaccgc cggcctgcgt ctggcacgtc agcgtgacca gcgcgctcga tcccagcagc
      721 cgtgattgca cgtccacgtc cagcgtgtag agcgcgcggc cgctggtctt gacctgcgtg
      781 acgactgcgg tgccggcccg cgactgattg gtgatcgcga ccgacgtgcc gtcctcgctc
      841 gactggactg gcgagcggcc ggccgggatg ccccaggctg cggcgttctg gtatgtcgcg
      901 acacccggta ccgggtcacc gagggcagct cccggccgcg cgtaaagatc gaggaagacg
      961 aggccgcgag gcgcgtcgta gaccttgtcg atgatgcgca catagcctgc tcgctcgatt
     1021 ccggcccgga cgaactcctc gccgcgccgc tggtaataga ggtaccagat cacgtcgtta
     1081 tcaggcgtat ccggcaggag ctcgctcagc ttgccggcat cgacatcggc tatcgtgatg
     1141 cccttctgcg ggatcgtgtc gttggacagc gtcttttcga gctcgccatc gcgcaccgta
     1201 atcagccgca tcgagtcgag caagcccggc tcgtacgcct cgacgagcgt gtccgccacc
     1261 gcgccgtagg agtaggtcac gaccgggaac tcgagcgggc tgaccatcgc caggtcggcg
     1321 gaggcgtcat cccagcgctg cttgaccgcg ctggcgtgaa tgttcccgat cgtgccaagg
     1381 cagagcacga gcacgaccag caggctggcg gcggcgagag gcgtgcgctg gcgattgatc
     1441 cggccgttga aggccgcccc gagcaagagc gaccagccga ccgctgcgct caggatcgtc
     1501 cgctcggcga agccggggct gagcaagctg acgatcaccg caaccacgat gcagcccagg
     1561 aagctgaaga cgaccgccat cgctgtccag cgctgccaca ggccgaccac gccgaggatg
     1621 actaccggcg ccatcgcgat caggatcagc gactggagat cgtgcagcct gtcccacggc
     1681 gtcttcgcga ggctcgggaa ataggctccg cggttgtccg agctgactcc ggtgatgcgc
     1741 aggacgataa gcatgatccg cttgaacccc gcgccgaggt aatcggcccg gcgctcgtcc
     1801 tcgttcgcgg agttgaccga gttccagacc tgtggcagcc agggcacgta cgcaaggact
     1861 gccagaccgg ctgcaatcag cagcgggatc atcttgcccc ggtatcgcca gacgtaatac
     1921 agcagcaccc ccgcctgtgg cccgagcacg aagagcgagc tgtaatcggc atagaccgcg
     1981 acggcgagcg acaggaaata gagcgccgcc cagcgccggt ccagtgtctg gatgaatccg
     2041 accagcgcca ggaacgaggc cgtgaccgac agcacgacca gcgagtacat ccgcgcttcc
     2101 tgcgagtagt agatgtgaat tggcgagagc atgaaaaccg tcgtcgcgac cagggccgcg
     2161 accgggtcga gcaggcggcg agccagcagg taaaagaccg gcagcaccag gacgccgcag
     2221 agcaccgcga cggtgcgccc tgccgagaca tcgggcagga cgagattgaa caccttgatc
     2281 agggtgaagt agaggggcgg atgcgggctg tagaagccgt ggaacccggt cgcggcggcc
     2341 caatcctgct tcgaatagag cagcgagaag ccctcgtcca gccacatgct caaccggttg
     2401 tagccccaca cccgcaggac cgccccgacc ccgatcgcga aagccaccgc cgcggcgtaa
     2461 acccgggccg gtgtcagcca ttgcagggtc ggggcgaatc gctcgccgac cacctcgatc
     2521 cgcgaaccgg gcagcgccat ggtccccgcc gccggggcga agctgagggc cgggcgaacc
     2581 cgctcaccga cgatcagctc ctgggccgcg aagccgatca ggatcaggcc gagtgggtgc
     2641 aagtggagca ggtaacgctc gttgttttca ttcgcgaagg cggccacaaa cagaatcggc
     2701 agccagtaga gcgtcatcag gatcgccagc acgttgcgcc ggcgcacctt ccagccggcg
     2761 aggttggtat ccaggaagta gcggccga
//
LOCUS       DASSGX010000331         4256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_493378, whole genome shotgun sequence.
ACCESSION   DASSGX010000331 DASSGX010000000
VERSION     DASSGX010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493378"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VFI12_05850"
     CDS             complement(<1..190)
                     /locus_tag="VFI12_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055966.1"
                     /translation="MTRFSRRTLLAGAAAVPIIGAASVQAQTNSPITPLEPSVTPTDS
                     GTVTSSARYFGDTGHNLKA"
     gene            339..1331
                     /locus_tag="VFI12_05855"
     CDS             339..1331
                     /locus_tag="VFI12_05855"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HET7055967.1"
                     /translation="MQSVLAHVTRGDCVESVHHGSIAVVDQNGKLVAHAGNPDLFLYF
                     RSSAKPIQAIPVVESGAADRFGFTPAELALCCASHSGTPRHQKQVLAMLAKLGLNDGA
                     LQCGSPAPYDDTAFSRVEAGLDPRTPLGCDCSGKHTGMLAACLQLGFPIDDYLNPNHP
                     LQQSILDLIAEVCAVDQDSIALATDGCSLPTFGSTLRAFARSYAVLADPQIHREALTR
                     LRDAMMAHPDNVGGPGQHVTDLMAAGKGAIVAKSGAEGLICIGIPEHGLGIAIRIADG
                     SFRAHPVVTAEVLRQLDVVPQSVLDAVLELHDPTIYNHNRRVVGEIRPAFTLSA"
     gene            1347..2384
                     /locus_tag="VFI12_05860"
     CDS             1347..2384
                     /locus_tag="VFI12_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET7055968.1"
                     /translation="MTAGLEGNHWAERVVAVMRSTVSHLAERAPLLAEMAEYHLGWTD
                     DMAGQERYRGKMIRPLIAVYAARAVGGVDEAEAAVPLAAAIELLHNFTLIHDDIQDNS
                     PSRRHRPTVWAKWGEAQAINAGDALFAAAHLALLEASEAGVPAAVVIDLIRGFDATTI
                     EIVSGQVLDLQFEQRQDVTSAEYIRMITGKTARIVEFAAGGGAWVGGAEADLAGHLAA
                     FGLALGLGFQVQDDLLGTFGASAETGKAAADDIRRKKQSLPVLLLRQAASPDHRARLA
                     AIYERPELDPDSVGEVLALMARYQIEEQVRQFVTEYHDQAVAALTATRLGDNMLRPLL
                     DLTRRLESRTS"
     gene            2780..3058
                     /locus_tag="VFI12_05865"
     CDS             2780..3058
                     /locus_tag="VFI12_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055969.1"
                     /translation="MQLVWTLPSPEEETAPPIQAFIGQYQDMLRAVGAWVDARGFRLT
                     GISGSGDALLVEVETSAPGDDAHREAFRLDYVAIERLVRAAQLDRNRF"
     gene            3254..4174
                     /locus_tag="VFI12_05870"
     CDS             3254..4174
                     /locus_tag="VFI12_05870"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET7055970.1"
                     /translation="MEERFRAMASRRGLITRGAAVTGAAVAMTGFSGHTARAIEDPGQ
                     SRLKQILDAGKIRVGTGSTNPPWHFEDENGKLVGFDIEMAKIVAGGLFGLTQEQVLGD
                     EEPRKHIEFVVHEADARIPDLMADKVDINFQFMTVTAGRALQVEFTIPYYREGVGLLF
                     LKDSPYNTRADLEGKGSNISILQNVYSEEIVHRGVSDANVQQFDSVAAAIEALDSSRV
                     DAAASDLSTLAWLAKNNGDKYKVDTNGWDPNSYAASIKPGDQIFLNFVNTAIQESMVG
                     LDFPLYKQAFNTYFGVDLQPAPTGFPAEWR"
BASE COUNT          741 a         1445 c         1312 g          758 t
ORIGIN      
        1 gcgctttcag gttgtggccg gtatcgccga agtatcgtgc cgagctggtc acggttcctg
       61 agtcggtcgg tgtcacgctc ggctcgagtg gcgtgatcgg gctattggtc tgggcctgga
      121 ccgatgcggc gccgatgatc ggcacagccg ccgcgccggc aagcagtgtt cgtctggaga
      181 atcgtgtcat caaggctgtc ctgtgcattc ggctcgtcct gctatttaaa cgagttcagc
      241 cggggcgtgg tttccggctc aggcaggttt gccgcgtttc gcgtagagtg caaagtcgac
      301 atgtgctgat ccattcatcc tcgtgaaaga gcaaacaggt gcaatcggtt cttgcgcacg
      361 tgacccgtgg cgactgcgtc gaaagtgtcc atcacggctc gatcgccgtc gtcgaccaaa
      421 acggcaaact ggtcgcccac gccggcaacc cggacctctt cctttatttc cgctcgtccg
      481 caaagccgat ccaggctatc ccggtcgtcg agtccggcgc ggccgaccga ttcggcttca
      541 cccctgccga gttggccctc tgctgcgcat cccatagtgg tactccgcga caccaaaaac
      601 aggtcctggc catgctcgcc aagctcgggc tgaatgacgg cgcgcttcag tgtggcagcc
      661 cggctcccta cgacgatacg gccttctccc gagtcgaagc tgggctcgat ccgcgcacac
      721 ccctcgggtg cgattgctcc ggcaagcaca ctggtatgct ggccgcctgc cttcaactcg
      781 gctttccgat cgatgactac ctcaacccca accatccact ccagcagtcg atcctcgacc
      841 tgatcgccga agtctgcgct gtggatcagg attcgatcgc gctcgccacc gatggctgca
      901 gcctgccgac cttcggctcg accctgcgcg ccttcgcccg ctcgtacgcg gtcctggccg
      961 acccgcaaat ccaccgcgag gcgctgaccc gcctgcggga cgcgatgatg gcgcatcccg
     1021 acaacgtcgg cggacccggc cagcatgtca ccgacctgat ggcagcgggc aagggcgcga
     1081 tcgtcgccaa gtcgggcgcg gaagggctga tctgcattgg catccccgag cacggactcg
     1141 gcatcgcaat ccggattgct gacggctcgt tccgcgcgca ccccgtcgtc acggccgagg
     1201 tcctgcggca gcttgacgtg gtcccacaat cggtcctgga tgcagtgttg gaattgcacg
     1261 atccgaccat ctacaaccac aatcgccgcg tcgtcggcga gatccgcccg gcgttcacgc
     1321 tcagtgcgta gcgggacggg gagcgaatga ccgcgggact cgagggaaac cactgggcgg
     1381 agcgcgtcgt ggccgtgatg cggtcgacgg tttcgcacct cgccgagcgc gcgccgctcc
     1441 tggccgagat ggccgagtac cacctcggtt ggaccgacga catggccggc caggaacgat
     1501 accggggcaa gatgatccgg ccgttaattg cagtctacgc agcccgggcg gtcggcggtg
     1561 tcgacgaggc ggaggcagcc gttccgctcg ccgccgcgat cgagctgctc cacaacttca
     1621 ctctgattca cgacgacatc caggacaaca gcccgtcgcg ccgccatcgg ccgacagttt
     1681 gggcgaagtg gggcgaggcg caggcgatca acgccggcga cgccctcttc gccgcagcgc
     1741 acctggccct gctcgaagcc agcgaggcgg gggtgccggc cgcggtcgtg atcgacctga
     1801 tccgcggctt cgacgcaacg acgatcgaga tcgtttccgg ccaggtgctc gatctccaat
     1861 tcgagcagcg gcaagacgtg acctcggccg agtacatccg gatgatcacc ggcaagacgg
     1921 cccggatcgt cgagttcgcg gccggcggcg gcgcgtgggt cggcggcgcg gaggccgacc
     1981 tggccggcca tctcgccgcg ttcggcttgg cgctcgggct cggattccag gtccaggacg
     2041 acctgctcgg caccttcggc gcatcggccg agactggcaa agccgcggcc gacgacatcc
     2101 ggcgcaagaa gcagtcgctg ccggtcctcc tgctccgcca ggcggcgtcg ccggaccacc
     2161 gcgcccggct cgctgcgatc tacgagcgcc cggagctcga tcccgactcg gtcggagaag
     2221 tccttgcgct catggcccgg tatcagatcg aagagcaggt ccgccagttc gtgacggaat
     2281 accacgacca ggcagtggct gccctgaccg cgacccggct cggcgacaac atgctccgcc
     2341 ccctcctcga cctcacccgc cgcctggaat ctcgcaccag ctaggtcaca ggttttcggt
     2401 tttcggtttt cggttttcgg ttttcggaga cgagcgtacc ggcgcccgac gttgcccagc
     2461 atctcgttcc gcccggggtt gaaaccccgg gctcagaaca cgaagcctct ccgaggctgg
     2521 aatgcgtggc aagaggtgtc gcattgccat gcgacgctac cgtctacttc tcgattcctc
     2581 gcctgctcgc ctcattccta agcaccgcta accggttcga ccttggtatc gctgtgtccg
     2641 gacgcttagg ccatggctgc ccggcgcaag cggcatacga ttgcttcagg aacggacgct
     2701 ccggttcgga tacgctgccg ggattgacct ggcgcgccgc ggctcgtcgt gccacgcacg
     2761 ccagacagga ttgacctcga tgcagctcgt ttggaccttg ccctcacctg aggaagagac
     2821 cgcgcctccg attcaggcct tcattggcca gtaccaggac atgctgcgcg ccgtcggcgc
     2881 ctgggtcgac gcccgcggat tccgcctgac cggcatctcc ggctctgggg acgccctcct
     2941 ggtcgaggtc gaaaccagcg cacccggcga cgacgcccat cgcgaagcct tccgtctcga
     3001 ctacgtcgcc atcgagcggc tcgtccgcgc tgcccagctt gaccgcaacc ggttctgagc
     3061 accgcgccgc gttaccgggc gcgcgttccc catgtccgtc atccccattt ccaggtgttg
     3121 acagcattcc aggatcgcca tagcatcctg ccgtctcacc aggcggaccg tgcggtccga
     3181 tcaacgcacg gtgagagccg gaacggccgc acgcgcgacc gatggtccgg catacaggga
     3241 atgggaggtc gcaatggaag agcgatttcg tgcaatggcc agtcgtcgcg gattaatcac
     3301 gcgtggcgcg gcggtaaccg gcgcggcggt tgccatgacc ggcttctcgg gacacacggc
     3361 ccgggcaatc gaagatccgg gccagagccg gctcaagcag atcctcgatg ccggcaaaat
     3421 ccgggtcggt accggcagca ccaacccgcc atggcatttc gaggatgaga acggcaagct
     3481 cgtcggcttc gatatcgaga tggcgaagat cgtcgctggc ggcctcttcg gcctcacgca
     3541 ggagcaggtc ctgggcgacg aagagccacg caagcacatc gaattcgtcg ttcatgaggc
     3601 ggatgcgcga attccggacc tcatggccga caaggtggac atcaacttcc agttcatgac
     3661 ggtgactgcc ggccgcgctt tgcaggtcga gttcaccatc ccctactacc gcgagggtgt
     3721 cggcctcctc ttcctcaaag actcgcccta caacacgcgg gccgatctcg aaggcaaggg
     3781 ctcgaacatc tcgatcctgc agaacgtcta ctcggaagag atcgtccatc gcggcgtctc
     3841 ggacgcgaac gtccagcagt tcgactcggt cgcggctgcg atcgaagcgc tcgactccag
     3901 ccgcgtcgat gctgccgcct ccgatctttc gacgctggcc tggctcgcca agaacaacgg
     3961 cgacaagtac aaggtcgaca cgaacggctg ggatccgaac tcatacgccg cttcgatcaa
     4021 gccgggcgat cagatcttcc tgaacttcgt caacaccgcg attcaggaat caatggtcgg
     4081 gctcgacttc ccactctaca agcaggcctt caacacctac ttcggagtcg atctccaacc
     4141 ggcgccgacc ggattcccgg ccgagtggcg gtaacgagat aacctggcaa tcatcgcctc
     4201 ccgggctgcg tatagcccgg gaggcgatga ccatttgaaa ccggatgtct tgtgta
//
LOCUS       DASSGX010000332         2366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1366150, whole genome shotgun sequence.
ACCESSION   DASSGX010000332 DASSGX010000000
VERSION     DASSGX010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1366150"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VFI12_05875"
     CDS             complement(<1..578)
                     /locus_tag="VFI12_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HET7055971.1"
                     /translation="MARMIGIDLGTTNSVMATIEGGEPVVIPNAEGERLTPSVVAMSA
                     QGERLVGRFARRQAVTNPENTIFSIKRFMGRKFTDPHVQKDLSLIPYKVREASNGGVE
                     VKMGDRWYSPQEISAMILQKMKADAEAYLGDKVDRAVVTVPAYFDNSQREATKDAGKI
                     AGLTVERIINEPTASALAYGLDKKHDEKVAVY"
     gene            complement(630..1163)
                     /locus_tag="VFI12_05880"
     CDS             complement(630..1163)
                     /locus_tag="VFI12_05880"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HET7055972.1"
                     /translation="MSMKEDMLAQETDIVEEQEGTASDQSAIESLTAERDQLIDQLQR
                     SVAEFQNYRRRNEQDRFKMREIATRDVLLSILPPIDDLDRAIRNIPDEEQMNSLREGL
                     LAIERKFLNVLERNGVTQVGSAGDEFDPAFHEAVATDESGERSHVVEVYQTGYRQGDA
                     SLRPAMVKVGGKPTFQA"
     gene            complement(1168..2229)
                     /gene="hrcA"
                     /locus_tag="VFI12_05885"
     CDS             complement(1168..2229)
                     /gene="hrcA"
                     /locus_tag="VFI12_05885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HET7055973.1"
                     /translation="MDQELSQTQPHEPVLTLRQRDILRMVIYEYVQTGRAVPSKTLVE
                     KYRVPFSSATIRNEMAVLEEFGLLQVLHTSGGRIPTDAGYRFYVHHLMGNVELPAGDQ
                     IMIRHQFQQAADRLDAWVELAATVLASASGNVSLVTAPRTIVPRLRHFELIQLQPSVA
                     LIVLVTFESAVRQSMIHLPVETTQDVLSRLADGLAPDLRGLTADEIEHRATGSDPIAR
                     EVLSQVCAMLHQFESAEQIEIRHSGLEHMVGQPDVADLDLQMMMSLVSSGGLMSALLP
                     QISSAGDVQVFIGSDDLPSGLNRFGVVLSPYGVADVMTGVLGVMGPTRMSYWRTISTV
                     RYMAALMSDLMAGLNPRLD"
BASE COUNT          429 a          757 c          696 g          484 t
ORIGIN      
        1 tcgtagacgg cgaccttctc gtcgtgcttc ttgtcgaggc catacgcaag tgccgaggcc
       61 gtcggctcgt tgatgatccg ctcgacggtc agcccggcga tcttgccggc gtccttggtt
      121 gcctcacgct ggctgttgtc gaaataggcc ggaacggtga ctaccgcgcg gtcgaccttg
      181 tcgccaagat aggcttcggc atcggccttc atcttctgca ggatcatcgc cgagatttcc
      241 tgcgggctat accagcggtc gcccatcttg acctcgacac cgccgttcga ggcctcgcgg
      301 accttgtacg ggatcagcga gaggtccttc tggacgtgcg ggtcggtgaa cttgcgaccc
      361 atgaatcgct tgatcgagaa gatcgtgttc tcgggattcg tgaccgcctg ccggcgcgca
      421 aagcgaccga caagtcgctc gccctgggcg ctcatcgcca ccacggacgg ggtgaggcgc
      481 tcaccttcgg cgttcggaat gacaaccggc tcgccacctt cgatggtggc catcaccgag
      541 ttcgtcgttc ccaggtcgat tccaatcatt cgtgccattg gtctgttttc cttttccttg
      601 ctctgtccgt cttaaccgtt gcctgtcagc tatgcctgaa atgtgggctt gccgcccact
      661 ttgaccatcg ccggtcgcaa cgaagcgtcc ccctggcgat agccggtctg atagacctcg
      721 acaacgtggg atcgctcgcc gctttcatcc gtcgcgaccg cttcgtgaaa tgcgggatcg
      781 aactcatcac ccgccgaccc gacctgcgtc acgccgttgc gctcgagcac gttcaagaac
      841 ttgcgctcga tcgcgagcaa cccttcccgg agactgttca tctgctcttc gtcggggatg
      901 ttcctgattg cgcggtcgag gtcgtcaatc ggcggcagaa tcgacaggag cacatcgcgc
      961 gtcgcgatct cccgcatctt gaatcggtcc tgctcgttgc ggcggcgata gttctggaac
     1021 tccgccacac tgcgctgcag ttgatcgatc agttgatcgc gctcagcggt cagcgattcg
     1081 atcgcggatt ggtcggaagc cgttccttcc tgctcctcca cgatgtcggt ctcctgtgcc
     1141 aacatgtcct ccttcatgct catgtcctta atccaaccgc gggttgagac cggccatcag
     1201 gtcgctcatc aacgccgcca tatagcggac tgttgaaatc gtgcgccagt acgacatgcg
     1261 ggtcggcccc atcacgccga ggacgccggt catgacatcc gcgaccccat acggcgaaag
     1321 cacgacgccg aagcggttca acccactcgg caggtcgtcg ctgccgatga agacctgaac
     1381 gtccccggcg gacgaaatct ggggcagcag ggcagacatc agtccccccg aactcaccag
     1441 actcatcatc atctgcaggt cgaggtcagc gacgtcgggc tggcccacca tgtgctcgag
     1501 accagagtgc cggatctcga tctgctcagc cgactcgaac tggtggagca tggcgcaaac
     1561 ctggctcagg acctcgcggg caattgggtc ggacccggtc gcccggtgct caatctcgtc
     1621 cgcggtcagg ccgcgcaaat ccggcgccag gccatccgcc aaccgggaca acacgtcctg
     1681 cgtcgtctcg accggcaaat gaatcatgga ctgccgcacg gcgctttcaa aggtgaccag
     1741 gacgatcagg gctaccgacg gctggagctg aatcagctcg aaatggcgca aacggggcac
     1801 gattgtgcgc ggcgcggtca ccagcgagac attgccgctg gccgacgcca gcacagtcgc
     1861 ggcgagttcg acccaggcat cgagccgatc ggccgcctgc tggaactggt gccggatcat
     1921 gatctggtcg cccgccggca gctcgacgtt acccatcagg tgatggacgt aaaagcgata
     1981 gcccgcgtca gtcgggatgc gtccacccga ggtgtgcagt acctggagca agccaaactc
     2041 ctcgagcacg gccatctcgt ttcggatggt cgcggaggag aacggaacgc ggtacttctc
     2101 gacaagcgtc ttcgacggga ccgcgcgacc ggtttgcacg tactcgtaaa tcaccatgcg
     2161 caaaatgtcg cgctggcgca gcgtgagtac gggctcgtgc ggctgggttt gactcaattc
     2221 ctggtccatc tgcgcggttg cttcccctgt tagcactctt gattgcagag tgctaacctc
     2281 aaataggata gcaactttac acggaccgtg tcaaggttcg gggcgccatt tcacccctgg
     2341 tttcaccgac gggatgggtg aattat
//
LOCUS       DASSGX010000333         2101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1038739, whole genome shotgun sequence.
ACCESSION   DASSGX010000333 DASSGX010000000
VERSION     DASSGX010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038739"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..1790
                     /locus_tag="VFI12_05890"
     CDS             234..1790
                     /locus_tag="VFI12_05890"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HET7055974.1"
                     /translation="MAAPAPGPEQARPVILRAGGIEKRYGGIVALRGVDLEIAQGEVH
                     ALMGENGAGKSTLAKIIAGVQTADAGTIEWQGRPVSFRATTEARDAGIAIVLQELNLI
                     PDLSVAENIFLTHPESYAGGLFLKRRDINQRTHELFDRLGWELPIDPAQKVSELSVAE
                     QQMVEILRALSWEARLYILDEPTATLSSREVDVLFGMIRRLQEQSISFLLVTHRLEEV
                     FAISDRITVYRDGANSGEFVTSQTTEQELIRAMVGRDLGDFFGVRTRQEPGDPILTVT
                     NLCRGQRLRNCSLTVRRGEVVGIAGLVGAGRTELIRAIFGADKADKGEVQLRGRAGLV
                     GAPSQAIRESTAMVPEDRKAHGLLVDLPIQHNVAMVGLTTSRGFWLRKRAERDLMTQM
                     TEHLQIRATDPQKPAGSLSGGNQQKIVLAKWLAIDPDLLILDEPTRGIDVGTKYEIYK
                     LIDGLVAQGKGVLLVSSELPEVLALSDRIIVMRDGKLVGELSHDEADEASILALAAQG
                     SLIEEKAEVA"
     gene            1793..>2101
                     /locus_tag="VFI12_05895"
     CDS             1793..>2101
                     /locus_tag="VFI12_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055975.1"
                     /translation="MAHAAAAPIGQRSRRGSTRFIEEYRVELAMLGVLILLGLVLSVI
                     ADNFLTERNLRNVMWQSTAIGIVAIGQTLVILTAGIDLSVGGMAALSAMVGGKAMMET
                     N"
BASE COUNT          383 a          705 c          694 g          319 t
ORIGIN      
        1 ggatcttcct ggcgccggag atcatgcggt tgctggagga gtaagcggtc caccgtcgcg
       61 caagacaatc gccgtccgcc cgaatcccga atcccgaatc ccgaacatcg aacatcgaac
      121 atcgaaccat tagcacccaa aaacccgagt ctaatcccta taatgaactg aagatcagtg
      181 ccaccgagtc atccgccggc catccccggg ttggctcgtc caggagttaa cgtatggcag
      241 caccggcccc cggcccggag caggcccggc ccgtcatcct gcgggcgggc ggcatcgaga
      301 agcgctacgg cggcatcgtc gccctgcgcg gtgtcgacct ggagatcgcc cagggcgaag
      361 tccacgccct gatgggcgag aacggcgcgg gcaaatccac ccttgccaaa atcatcgccg
      421 gcgtgcagac ggccgacgcc ggcaccatcg agtggcaggg ccggccggtc tccttccgcg
      481 ccaccactga agcgcgagat gccggcatcg cgatcgtcct ccaggagttg aacctgatcc
      541 cggacctctc ggtcgccgag aacatcttcc tgacccatcc cgagagctac gccggcggcc
      601 tcttcctgaa gcggcgcgac atcaaccagc gcacgcacga gctcttcgac cggctcggct
      661 gggagttgcc aatcgacccg gcccagaagg tcagcgaact ctcggtcgcc gagcagcaga
      721 tggtcgagat cctgcgcgcc ctctcctggg aggcgcggct ctacatcctc gacgagccga
      781 ctgccaccct cagcagccgc gaagtcgatg tcctcttcgg catgatccgc cgcctccagg
      841 agcagagcat cagcttcctg ctcgtcaccc accggctgga ggaggtcttc gccatcagcg
      901 accggatcac ggtctaccgg gacggcgcca actccggcga gttcgtcacc agccagacga
      961 ccgagcagga gctgatccgc gcgatggtcg ggcgcgacct cggcgacttc ttcggcgtcc
     1021 gcacccggca ggagccgggc gacccgatcc tgactgtgac caacctctgc cggggccagc
     1081 gactgcgcaa ttgctccctg acggtccggc gcggcgaagt ggtcgggatc gccgggctgg
     1141 tcggggccgg gcgaacggag ctgattcggg ccatcttcgg ggcggacaag gccgataagg
     1201 gcgaggtcca gctccgcggc cgggcggggc tcgtcggcgc tccgagccag gcgatccggg
     1261 aatcgaccgc gatggtaccg gaggaccgta aggcgcacgg cctgctggtc gatttaccga
     1321 tccagcacaa cgtcgcgatg gtcgggctga cgaccagtcg gggcttctgg ttgcgcaaac
     1381 gggccgaacg cgatttgatg acgcagatga ccgagcatct ccagatccgg gccaccgatc
     1441 cgcaaaagcc ggccggcagc ctgagcggcg gcaaccagca gaagatcgtg ctggccaagt
     1501 ggctcgcgat cgacccggac ctgctgatcc tggacgagcc gacccggggc atcgatgtcg
     1561 gcacgaagta cgagatctac aagctgatcg acggcctcgt agcccagggc aagggggtcc
     1621 tgctcgtctc ctccgagttg ccggaggtgc tggctctctc cgaccggatc atcgtcatgc
     1681 gcgacggcaa gctggtcggt gaactgagcc acgacgaagc cgatgaggcg tcgatcctgg
     1741 cgctcgcggc gcaggggtcg ctgatcgaag agaaggcgga ggtggcctag cgatggcgca
     1801 cgcggcggca gcgccgatcg ggcagcggtc ccggcgcgga tcaacccggt tcatcgagga
     1861 gtaccgcgtc gagctggcga tgctcggcgt cctgatcctg ctcggcctgg tgctgagcgt
     1921 catcgccgac aacttcctga ccgagcgcaa cctgcgcaat gtcatgtggc aatcgaccgc
     1981 gatcggcatc gtcgcgatcg gccagacgct ggtgatcctg accgccggga tcgacctttc
     2041 ggtcggtggc atggcggcgc tctcggcgat ggtcggcggc aaggcaatga tggaaacgaa
     2101 c
//
LOCUS       DASSGX010000334         4903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_59905, whole genome shotgun sequence.
ACCESSION   DASSGX010000334 DASSGX010000000
VERSION     DASSGX010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4903
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59905"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VFI12_05900"
     CDS             <1..437
                     /locus_tag="VFI12_05900"
                     /inference="COORDINATES: protein motif:HMM:NF040269.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055976.1"
                     /translation="RTEIVVSYLALTPGTPHLASGWTWLPVSSLNGRIAHPDQVVLDY
                     AIVRLQAKIGYTTIAFHLMPQSFSLSDLQLTYETILGRLLDPRNFRRRVVSSRLLQPE
                     NRLRREGSHRPAALYRFAGNHDPASFLTPAAPVTRDRDRTAE"
     gene            446..1297
                     /locus_tag="VFI12_05905"
     CDS             446..1297
                     /locus_tag="VFI12_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HET7055977.1"
                     /translation="MTQSIGIIAAFSKPEASELAVRIREHLESLGCDVTPEQELAQAD
                     PAGLQAIVVLGGDGLMMRAASMYPDVPLFGINFGKVGFLAMVEQRDWLIAINALLAGL
                     YTVQDAATLAASVSRGANTLDQGWAINDVVIRGGVRMIDLEVYIDGHYVNTYPGDGMI
                     VSTPRGSTAYCMAAGGPILTAGVQGFAIVPISCHSPIRTPFIVAESALIELVVATKHD
                     CSLILDGRQTAELSEQDVVRVQRGEHQFRLVTLDSMDFYEAFRTKFNFKIRPDAVPSR
                     VQATTGS"
     gene            complement(1294..2085)
                     /locus_tag="VFI12_05910"
     CDS             complement(1294..2085)
                     /locus_tag="VFI12_05910"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET7055978.1"
                     /translation="MEATIARALQVLIALSGAYLAALWVVLIVWTYRDIESRSRSVVT
                     QVFSTMLSALFFVPGVLLYMMLRPKETLDLTFQRSLEEEYLLQDLETNVSCPHCNRAI
                     SDEFVVCPHCHTKLKEACENCSREIDVRWSLCPYCGHEKGGPLAEIRPVIQPIERFVQ
                     SDEEPIVRELPTTQAIEQLEAQAFYRMEGPPAPAAIATNAGRAFDRRKTREMHRARNL
                     NGRTEHAPSPNGNGHENGVSEASLNAKGDTAETSEVGSLSTDTEV"
     gene            complement(2114..2446)
                     /locus_tag="VFI12_05915"
     CDS             complement(2114..2446)
                     /locus_tag="VFI12_05915"
                     /inference="COORDINATES: protein motif:HMM:NF017920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HET7055979.1"
                     /translation="MKLIFAVTERGLADSAMSELTGRGFPVTMVHEAGGLLVPGQGTL
                     MLGVPDGAVEFVLNVLDRVCGTRLRQVNALLPGSDPTDLMISEQSSVIEGGVSIFVVP
                     VRRFERIA"
     gene            complement(2443..3081)
                     /gene="tmk"
                     /locus_tag="VFI12_05920"
     CDS             complement(2443..3081)
                     /gene="tmk"
                     /locus_tag="VFI12_05920"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HET7055980.1"
                     /translation="MRTGTFIAFEGIDGSGKSTAMRVVAERLRERGLDVVATREPGGT
                     PPGRQIRALLLDADHALTPATELLLMCADRAEHVATVIRPALQAGKIVLCDRYAGSTR
                     AYQGYGLGLDMRTVDAAIAIATGGLSPDLTILFDLEPSAAHARRSGDAGSLNQLDVRD
                     LAFRTRVRDGFLALAGSSSDWRIIDASQSTERVADMALELIDLIIAGQRVVQ"
     gene            complement(3083..4777)
                     /locus_tag="VFI12_05925"
     CDS             complement(3083..4777)
                     /locus_tag="VFI12_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HET7055981.1"
                     /translation="MIDNLDEVLLALPVPIADAVREELAAESHGPLIEIVMDLGRRPE
                     ARFQNGESYLSDRDVTRDDLQHVVDRIGVFGDDNRAGIERTLHRISAIRNRTGAIVGL
                     TCRVGRAVYGTISIIRDLVESGESVLLLGRPGVGKTTMLREVARVLADDLGKRVVIVD
                     TSNEIAGDGDIPHPAIGRARRMQVPTPTAQHAVMIEAVENHMPEVIVIDEIGTELEAM
                     AARTIAERGVQLIGTAHGNTLENLMMNPVLSDLIGGIQSVTLSDEEARRRGTQKSILE
                     RKAPPTFNVMVEIINRDEVSVHRDVAMTVDSILRGIAARPELRKRDESGELAIVNSVP
                     PPPGPGSDYAPHPRRSAVREVPTTTLREVPPQVRSMLSPEPAVQAGMASVTPLPRGRG
                     TPQKPIRIFPFGVSRNRLEQAIAQTRVHATIVRDLREADIVITLKNYFRQKPAPIRDA
                     ETRGVSVYVLRANTIVQMESVLSSLMPAAHRPAESAAPSRPEREQEQSNMLGALEEAE
                     QAIGMVIEGAPPISLTPQESYIRKLQHQLADRYNLGSRSKGREPHRHVEIYRAGIK"
BASE COUNT          869 a         1593 c         1526 g          915 t
ORIGIN      
        1 cccgcaccga gatcgtcgtg agttacctgg cgctcacccc tggcacgcca cacctggcca
       61 gtggctggac ctggttaccg gtgagcagcc tgaacggcag aattgcgcat ccggatcaag
      121 tggtgctgga ttacgcgatc gtgcgcctgc aggcaaagat cggctacacg acgatcgcct
      181 tccacctcat gccccagtca ttcagcctga gcgacttaca attgacctat gagacgatcc
      241 ttggccgact gctggatcct cgcaacttcc ggcggcgcgt ggtttcgtcg cggctgttgc
      301 agccggagaa ccgcttgcgt cgggaaggaa gccaccgtcc ggccgcgctc tatcgctttg
      361 ccggtaatca tgatcccgcc tctttcctga cacccgccgc gcccgtgact cgggatcgtg
      421 atcgcaccgc cgaatgagga atgccatgac ccagtcgatc ggaattattg ccgcattcag
      481 caagccggag gcgtcggaac tggcggttcg tatccgcgag cacctggagt ccctcggatg
      541 cgatgtgacg cccgagcagg agctggccca ggcagatccg gcgggcctgc aggcgatcgt
      601 ggtgctcggc ggcgacggtc tgatgatgcg ggccgccagc atgtaccccg atgtgccgct
      661 gttcggcatc aacttcggga aggtcggctt cctggcaatg gtcgagcagc gcgactggct
      721 gatcgcgatc aatgccttgc tcgccgggct ctacactgtg caggatgctg cgacactggc
      781 cgcttcggtc tccaggggag ctaacaccct cgatcagggc tgggcgatca acgacgtcgt
      841 cattcgcggt ggggtccgca tgatcgacct cgaggtctac atcgatgggc attacgtcaa
      901 cacctacccc ggcgacggca tgatcgtctc gacgccgcgc ggcagcaccg cctactgcat
      961 ggcagccggc gggccgatcc tgaccgccgg agtacaaggg ttcgcaatcg tcccgatctc
     1021 gtgccattct ccgattcgca caccgttcat cgtggcagaa tccgccctga tcgagctcgt
     1081 ggtcgccacg aagcatgatt gctcgctgat cctcgatggg cggcaaacgg cggagctgag
     1141 cgaacaggat gtcgtccgcg tacagcgggg cgagcatcag tttcggctgg tcacgctcga
     1201 ttcaatggac ttctacgagg cattccggac caagttcaac ttcaagatcc ggccggacgc
     1261 cgttcccagt cgcgtacagg cgacaaccgg ttcctagacc tcggtgtcgg tcgataggct
     1321 gccgacttcg gaagtctcgg ccgtgtcgcc cttggcattc agagatgcct ctgacacgcc
     1381 gttctcgtgg ccgttgccgt tcgggctcgg ggcgtgttcg gtgcggccat tgaggttgcg
     1441 cgcccgatgc atctcccgcg tcttgcgccg gtcgaacgcg cgtcccgcgt tggtcgcgat
     1501 cgccgccgga gcgggcggcc cttccattcg gtagaacgcc tgcgcctcca gttgctcgat
     1561 tgcctgggta gtgggcagct cccggacgat cggctcctcg tcgctctgca cgaaccgctc
     1621 gatcggctgg atcaccgggc gaatctccgc cagcggtccg cccttctcat gtccgcaata
     1681 ggggcaaagc gaccagcgca catcaatttc gcgactgcag ttttcgcacg cttccttgag
     1741 tttcgtgtga cagtgtgggc agacgacgaa ctcgtcgctg atcgcgcgat tgcagtgcgg
     1801 acacgacacg ttggtctcca ggtcctgcag gaggtattcc tcctccagcg agcgctggaa
     1861 tgtgagatca agcgtttcct tgggccgcag catcatgtag agcagtacgc cggggacaaa
     1921 gaagagcgcc gaaagcatgg tcgaaaagac ctgcgtgacg acgctccgac tccgggactc
     1981 gatatcccgg taggtccaga cgatcagcac gacccagagc gccgccaaat aggcgccgct
     2041 cagagcgatc aatacctgta atgctcgggc gattgttgct tccatcagcc tgcttcgtct
     2101 gccggcagat gcgctacgcg atccgttcga accggcgcac tggcacgacg aatatcgaga
     2161 ctcctccttc gatgaccgag gactgctctg agatcatcag atcggtcggg tccgatccag
     2221 gcaacaacgc gtttacctgg cggaggcggg tcccgcacac gcgatcaagc acattcagga
     2281 cgaattcgac ggccccgtcg ggcacgccga gcatcagtgt accttgcccg ggcaccaaca
     2341 aaccgcctgc ctcatggacc atcgtcacgg ggaaaccacg gccggtcagt tcggacatcg
     2401 ccgaatcagc caacccccgt tcagtaactg caaagattaa cttcactgca cgacccgctg
     2461 tccagcaatg atgaggtcga tcagctccag cgccatgtcc gccacacgtt cggtggattg
     2521 cgacgcgtcg ataatgcgcc aatcgctgga tgagcccgcc agcgccagga aaccgtcgcg
     2581 cacgcgcgtc cggaatgcga ggtcccggac atccagttgg ttcaggcttc cggcgtcgcc
     2641 ggatcggcgg gcgtgcgccg cggatggctc gagatcgaac aggatcgtaa gatcgggcga
     2701 cagaccgccc gttgcgatcg caatggccgc gtcgacagtg cgcatgtcga gaccaaggcc
     2761 gtagccctga taggcgcgag tcgagccagc gtaccggtcg cagagcacga tctttccggc
     2821 ctgcaaagca ggccggatca ctgttgcgac gtgctctgcc cggtcggcgc acatcagaag
     2881 gagctcggtg gcgggagtca gcgcgtggtc ggcgtcgagc agcagtgcgc gaatctgccg
     2941 gcccggcggg gtgccaccgg gctcccgcgt cgcgacgacg tcgaggccgc gctcccgcag
     3001 gcgttcggcg accacgcgca tggcggtcga cttgcccgaa ccgtcgatgc cttcgaaggc
     3061 aatgaaggtg ccagtcctca agctacttga tcccggcgcg gtagatctcg acgtgccggt
     3121 gcggctcccg gcctttgctg cgcgagccca ggttgtatcg atcggcgagc tgatgttgca
     3181 gtttgcgaat gtacgactcc tgcggtgtca ggctgatcgg cggcgcgccc tcgatcacca
     3241 tgccgatggc ctgctcggcc tcttcgagag cgccgagcat attcgactgc tcctgctccc
     3301 gctccggacg gctgggagcc gccgactcgg ccggacggtg cgctgcgggc atcagtgaag
     3361 agagcacact ctccatctgc acgatcgtat tcgcgcgcag gacatagacc gacacgccac
     3421 gcgtctccgc gtcccggatc ggcgcgggct tctgccggaa atagttcttg agcgtgatta
     3481 cgatatccgc ttcacgcaga tcgcgaacga tcgtcgcgtg gactcgggtc tgcgcgatcg
     3541 cctgctcgag ccgattgcgg cttactccga aagggaagat acgaatcggc ttctgcggag
     3601 tacctcggcc ccgcggcaac ggcgtcaccg atgccatgcc agcctgcacg gccggttctg
     3661 gcgagagcat ggagcgaacc tgcggaggga cttcgcggag cgtggtcgtc ggaacttccc
     3721 gaaccgccga ccggcgagga tgaggagcat aatccgaacc aggtccgggc ggcggtggaa
     3781 cagaattcac aatcgccagc tcgccggatt cgtcgcgctt gcgcagctcg ggtcgcgccg
     3841 cgatgccgcg caggatcgaa tccaccgtca ttgccacgtc ccgatggacc gacacttcgt
     3901 cccggttgat gatctcgacc atgacattaa aggtaggcgg cgccttgcgc tcgaggatcg
     3961 acttctgtgt gccgcggcga cgcgcttcct cgtccgacag ggtgaccgac tggataccgc
     4021 cgatcagatc ggacagcacc gggttcatca tcaggttctc gagggtattg ccgtgagcgg
     4081 ttccgatgag ctgcacgccg cgctcggcga tcgtgcgggc cgccatagct tcgagctcgg
     4141 tgccgatctc atcgatgacg atcacttccg gcatgtggtt ctcgacagcc tcgatcatga
     4201 ccgcgtgctg cgcggtcggc gtcggcacct gcatccggcg cgcacggccg atggccgggt
     4261 gcgggatatc gccgtcaccc gcgatctcat tgctcgtgtc gacgatcacg acgcgcttgc
     4321 cgagatcatc ggccaggaca cgcgccacct cgcgcagcat cgtcgtcttg ccaacgcccg
     4381 gtcgaccgag cagcagcacg ctctcgccac tctcgacgag atcgcgaatg atcgagatgg
     4441 tgccgtaaac agctcgcccg acgcgacagg tgagcccgac gatcgcgccg gtccggttgc
     4501 gaatggccga gattcggtgc agggtccgct cgataccggc tctgttgtcg tcgccgaaaa
     4561 cgccgatacg gtcgacgacg tgctgaaggt catcgcgcgt cacgtcccgg tctgagagat
     4621 agctctcccc gttctggaaa cgcgcctcag ggcggcgacc aaggtccatc acgatttcga
     4681 tcagcgggcc gtggctctct gccgccagct cttcccggac ggcatcggcg atcggcaccg
     4741 gcagcgccag cagcacctcg tccaggttgt cgatcatctt ctggtccgcc ggccagccct
     4801 gaagcctcgt ggaacctgtc ttcagacgtg tttctatcaa ttcgacgtcc ccgtcacttg
     4861 aaaaatacgc attcgttcta tctcataagg cagggtactc acg
//
LOCUS       DASSGX010000335         2931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_932160, whole genome shotgun sequence.
ACCESSION   DASSGX010000335 DASSGX010000000
VERSION     DASSGX010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932160"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="VFI12_05930"
     CDS             <1..949
                     /locus_tag="VFI12_05930"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HET7055982.1"
                     /translation="TGKLEVRPNTRVTKILSNDQGQVTGVQYLDENGQEQEQPAGVVI
                     LNTYVYENVRLLLVSTSDFYPNGLGNNGGQVGKNYMSHAYVGRNGLFPGKKLHLFSGT
                     SGQAAAMDDLNGDNFDHTGLGFIRGAVVFASSGDLPIGKSRSVAPGVPQWGPEYKRWV
                     HENADSVGAVFAQVEPLPYEMNFLDLDPKVTDPIGMPVIRVTYDFGENENKATTYIDG
                     KLDELIKTIGATETWPGYPARTPLPINSHAYGGTRMGEDPAGSVVNKYGLAHEAPNLM
                     ILGGSTFPASSGYNPTETIEALAWFAADYLAQNLDAIAV"
     gene            1022..1276
                     /locus_tag="VFI12_05935"
     CDS             1022..1276
                     /locus_tag="VFI12_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055983.1"
                     /translation="MHRTLIVVAIAALGLGAGGYALAQDAGTPAATELCATPGAGPSG
                     TPAASPVAVAMATAAAGVPGANVGPAPSPTLLYECATPTK"
     gene            1491..1727
                     /locus_tag="VFI12_05940"
     CDS             1491..1727
                     /locus_tag="VFI12_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055984.1"
                     /translation="MKQIGVREFRDHATTYLSGPDPVAVSKHGRIIGFYIPLKRDEEA
                     VRRAIDKFGKAIDQVRQEAGLTEEELIDMLGLST"
     gene            1745..2179
                     /locus_tag="VFI12_05945"
     CDS             1745..2179
                     /locus_tag="VFI12_05945"
                     /inference="COORDINATES: protein motif:HMM:NF021634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HET7055985.1"
                     /translation="MADASVLVAEFLRERSRAFFSESELRVVVAEEQWSEFRHELERR
                     LAVIVQQGRIEPEQAMVIRQEIQRLINEHAIEVVPRHVYHHVETTARRRIPRDPNDWP
                     TVALALTLNAGILVNDNDFLSGGCPTWSFATLKAELEQGQPP"
     gene            2233..2739
                     /locus_tag="VFI12_05950"
     CDS             2233..2739
                     /locus_tag="VFI12_05950"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET7055986.1"
                     /translation="MVHPLVEQLRFARSEFRRGLEGVSDEDARRRFLPMNCLSWNVGH
                     LAAQEQRYWLSLAQGQVLLPEIDRAFRYGAPASTPALDDTFRAWETITAAADPWLDAV
                     TEETLLTRVTRDGQPTNVTFGSLLLRTTYHYWYHTGENAAIRQQLGHTALPEFVGNID
                     DEAPYRKE"
BASE COUNT          543 a          947 c          957 g          484 t
ORIGIN      
        1 aaccggcaag ctggaggtgc ggccgaacac ccgcgtgacg aagatcctca gcaacgacca
       61 gggccaggtc accggagtcc agtacctcga cgagaacggg caggagcagg agcagccggc
      121 cggcgtcgtc atcctgaaca cgtacgtcta cgagaacgtg cggctgctgc tggtgtcgac
      181 ctccgacttc tatcccaacg gcctgggcaa caacggcggg caggtcggca agaactacat
      241 gtcgcacgcc tacgtcggcc gcaacgggct cttcccgggg aagaagctcc atctcttctc
      301 gggcacgagc ggccaggcgg ccgccatgga tgacctgaac ggcgacaact tcgatcacac
      361 cgggctcggc ttcatccgtg gtgcggtcgt cttcgccagc agtggcgacc tgccgatcgg
      421 gaaatcgcgc agcgtcgcgc cgggcgtgcc gcagtggggt cccgagtaca agcgctgggt
      481 ccacgagaac gccgactcgg tcggcgcggt cttcgcccag gtggagccgc tcccctacga
      541 aatgaacttc ctcgacctcg acccgaaggt cacggatccg atcgggatgc cggtgattcg
      601 ggtcacctac gatttcgggg agaacgagaa caaggccacc acctacatcg acggcaagct
      661 cgacgagctg atcaagacga tcggcgcgac ggagacctgg ccggggtacc cggcgcggac
      721 gccgctgccg atcaacagcc acgcctacgg gggcacccgc atgggagagg atccggcggg
      781 ctcggtggtc aacaagtacg ggctcgcgca cgaggcgccg aatctcatga tcctgggcgg
      841 gtccacgttc ccggccagct ccggttacaa cccgaccgag accatcgagg cgctggcctg
      901 gtttgcggcc gattacctcg cccagaacct ggacgcgatc gccgtctagg acatctggcg
      961 gctgtttcct cggggcgagg catcactcgc tccgaggctg cccctggcaa ggagacccgc
     1021 catgcatcgc acgctcatcg tcgtcgccat cgccgcgctg gggctcggcg cgggtggcta
     1081 cgcgctcgcc caggatgccg gcaccccggc cgcgacggaa ctctgcgcca cgccgggcgc
     1141 tggcccgagc ggaacgcccg cggcgagccc ggtggcggtg gcgatggcca cggcagcggc
     1201 cggtgtgccg ggcgccaatg tggggccggc tccctcgccg acactcctgt acgagtgcgc
     1261 gacgccgacg aagtagggag ggaatacagc gtccgtgcac acgggtcatg ctgaggaacg
     1321 aagcatctcg gctcaattga agcccggttc cctgagtgcc gagattcctc cttcgtcgga
     1381 atgacacgag tttggcgcac tgctgcagtg tcacgacgtg gctctggtgg ttgaccgcgg
     1441 tctgcggctc acctagaatt gtgaccacga ggtgaccacg cctgaccacg atgaaacaga
     1501 ttggcgtccg cgagttccgc gaccacgcca cgacctatct ctccggacct gaccccgtcg
     1561 ccgtcagcaa gcacgggcgc atcatcggct tctacatccc gctcaagcgc gatgaggagg
     1621 cagtccgccg cgcgatcgac aaatttggca aagccatcga tcaggtgcgg caggaagcgg
     1681 ggctgactga ggaggagttg atcgacatgc tcggcctcag cacgtagctg acgccgtgct
     1741 gattgtggct gatgccagcg tcctcgtcgc agaattcctc cgtgaacgca gtcgtgcgtt
     1801 tttcagcgaa tctgaacttc gcgtggtcgt cgcggaagaa caatggagcg agtttcggca
     1861 tgaactggag cgtcgcttgg cggtcatcgt ccagcagggt cggatagagc ctgaacaggc
     1921 aatggtgatc cggcaggaga tccaaagact catcaatgag catgccattg aagtcgtccc
     1981 gcgacacgtg taccaccatg tggagaccac cgcgcggcgc cggattccgc gtgatcccaa
     2041 tgattggccg acggtcgcgt tggcgctgac actcaacgcg ggaatcctcg tcaacgacaa
     2101 cgactttctg agcggcggct gtccgacatg gtccttcgct acactgaaag cggaattgga
     2161 acaggggcag ccaccgtgag gcgactctgg ggataagggc tgtcgcttca atcatatggt
     2221 ccgaggtctc gcatggttca cccgttggtc gagcagttgc gctttgcccg cagcgaattc
     2281 cggcgtgggc tggagggcgt gtcggacgag gatgcgcggc ggcgcttcct gccgatgaat
     2341 tgcctcagtt ggaacgtcgg ccatctggcg gcgcaggagc agcgctattg gctctcgctc
     2401 gcgcaaggcc aggtgctcct gccggagatc gaccgcgcct tccggtatgg cgcgccggca
     2461 tcgacaccgg cgctcgacga tacgtttcgg gcatgggaga ccatcacggc cgcggccgat
     2521 ccctggctcg acgcggtcac ggaggagacg ctgctcacgc gcgtcacccg cgatggccag
     2581 ccgactaatg tcacctttgg ctctctgctg ctacggacga cctaccatta ttggtatcac
     2641 actggcgaga acgcggcgat tcgccagcaa ctcggacaca ccgctctgcc cgaattcgtc
     2701 ggcaacatcg acgacgaagc accgtaccgc aaggagtgac agcgcaaccg atgccgctgt
     2761 cccggcaacc accggcgtcg ggtcgtcccg ggctgacacc aacccgtcgt tagcggtttg
     2821 ccgtgagcgg gttcatccac gtgatccccg ggaaccgcga gaaatcgcgg tccgtcgtgc
     2881 agactgtggc gccgtgctcg agcgcgatgg ccgccagcac ggcgtccatg a
//
LOCUS       DASSGX010000336         2112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1149196, whole genome shotgun sequence.
ACCESSION   DASSGX010000336 DASSGX010000000
VERSION     DASSGX010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149196"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            302..613
                     /locus_tag="VFI12_05955"
     CDS             302..613
                     /locus_tag="VFI12_05955"
                     /inference="COORDINATES: protein motif:HMM:NF013999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HET7055987.1"
                     /translation="MTTPTLSPGQLLESLMQVIDPEVGINIVDLGLVYAVDLTGASAT
                     VEMTLTTPGCPLHASIEAAVKRALMTNHPELNEVNVQLVWDPRWNVDFITEEGRRQLG
                     W"
     gene            648..1481
                     /locus_tag="VFI12_05960"
     CDS             648..1481
                     /locus_tag="VFI12_05960"
                     /inference="COORDINATES: protein motif:HMM:NF021520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HET7055988.1"
                     /translation="MTQTTQPILASEKMSDVLKKHPALLETLIDLSPAFRNLRNPVLR
                     RVQTRLVTVSQAAGIAGIEPALLTQRLNEAAGIFAPSQTEPVTHVDETLGAAIDPMID
                     GAPVAAEIDVRPLMARGEEPFKAIMTAARTVPAGSTLRLVAGFEPLPLYDVLAKQGFI
                     HHTRRLEADAWEVRFYRERSDSEAETVASDTAASRTVDWAETPSSEITIDVSELVPPE
                     PMIRILEALESLPPEGRLLVHHVRRPIHLYERLDEMGYGYDTRELGPGRIEVLIQKRP
                     A"
     gene            1478..>2112
                     /locus_tag="VFI12_05965"
     CDS             1478..>2112
                     /locus_tag="VFI12_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055989.1"
                     /translation="MNGLNSANAPSLDLPARFMALSVIALATIGLTAPWTLPLMRGSF
                     SDFQLLAFVHLTTLGFVGAMIIGASYQLVPVALQVPLASARFGRISFWFYLAGLGFFL
                     TGLATEWLLALGIGGSLLAIAFLLYIGVIGATWFRAPERDVVAWHILLGLAGAGVGMT
                     LGFSLALNKSNGMLGPHLLNMLAAHITLMLGGWMLITFSGVAYRLIGMFTLS"
BASE COUNT          380 a          692 c          635 g          405 t
ORIGIN      
        1 cggaacgcca agtcgatcta ttggcaccgt tcgccaaaac gatgcgcccg ccaggagcga
       61 gacctgctcc tcgagcgtca tctgaccgac caggcggcga atctccgacc caaccatgcg
      121 cactccttcg tgtcgatcat cagcaaactg gccggttcca gtctatcccg ccgccgtatg
      181 ccgcggttga tccggcatca atgcatccag gtgcgattga tttcaggagc ggcgctcact
      241 aaaatacgcg tattccgacc aaataagtcg gatatctcgc acatcgcatc aggacgctgt
      301 catgactaca ccgacgcttt cgcccggaca gcttctcgaa tccctcatgc aggtcatcga
      361 ccccgaggtc ggcatcaaca tcgtcgatct tggcctcgtc tacgcagtgg acctgaccgg
      421 cgccagtgcg accgtcgaaa tgacgctgac gacgccgggc tgtccgctcc acgcctcgat
      481 cgaagccgcc gtcaagcgcg cgctcatgac caaccacccc gagctcaacg aagtcaacgt
      541 gcagcttgtc tgggacccac gctggaacgt cgacttcatc actgaggagg gacgccgaca
      601 actcggctgg taactgacga atcgcaggcc acgcacggga gagcaccatg acgcagacca
      661 cgcagcctat tctcgcgagc gaaaagatga gcgacgtcct caagaagcac ccggccctgc
      721 ttgagacgct gatcgatcta tcccccgcat tccggaatct ccgcaacccg gtgctccgcc
      781 gcgtccagac ccggctcgtg acggtcagcc aggcagcggg aatcgcgggc atcgagcccg
      841 ccctcctgac gcaacgcctg aatgaggcgg cgggcatctt cgcgccaagc cagaccgaac
      901 cggtcaccca cgtggacgag acgctgggtg cagccatcga cccgatgatc gatggtgctc
      961 cggtggcggc ggagatcgac gtccggccgc ttatggcgcg cggtgaggag ccattcaagg
     1021 cgattatgac ggctgcgcgg acagtgccgg ctggatcgac gttgcggctc gttgccgggt
     1081 tcgaaccgtt gccgctttac gacgtgctgg cgaagcaggg attcattcac cacacccgtc
     1141 gtctcgaagc cgatgcgtgg gaagtcaggt tctacaggga gcggagtgac tccgaggcgg
     1201 agacggtagc gtccgatacc gccgcctcca gaacggtcga ctgggcggag acgccgtcat
     1261 ctgagatcac gatcgatgtc agcgaactcg ttccccctga gccgatgatc agaatcctcg
     1321 aagcgctgga gagtttgccg cccgagggcc ggttgctcgt ccatcatgtt cgccgcccga
     1381 tccacctcta tgagcggctg gatgagatgg gatatgggta tgacacccgg gagctcgggc
     1441 cgggccggat cgaagtcctg atccagaagc gaccagcgtg aacgggctca attccgccaa
     1501 tgcgccgtcg ctcgatctgc cagcgcggtt catggcattg agcgtgatcg ctctggcaac
     1561 catcggcctg acagcaccgt ggacgctgcc gctgatgcgc ggctccttca gtgacttcca
     1621 gctcctcgcc ttcgtgcacc tcacgacgct cggatttgtc ggcgcgatga tcatcggcgc
     1681 gagttaccag ctcgttcccg tcgcgctcca ggtgccgctc gcctctgcac gattcggccg
     1741 gatctcgttc tggttctatt tggccggcct cgggttcttc ttgaccgggc tggcgaccga
     1801 gtggctgctg gccctcggca tcggtggatc gctcctggcg atcgcgttcc tgctttacat
     1861 cggcgtcatc ggagcgacct ggtttcgcgc gccggagcgt gatgtcgtcg cctggcacat
     1921 cctgctcggc ttggccggag cgggtgtcgg catgacctta gggttctcgc tcgcgctcaa
     1981 caagagtaac gggatgctcg gcccgcattt gctcaatatg ctggccgcgc acatcacgct
     2041 gatgctcggc ggctggatgc tgatcacgtt ttccggtgtc gcctaccgct tgatcggcat
     2101 gttcacgctg tc
//
LOCUS       DASSGX010000337         2302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1149228, whole genome shotgun sequence.
ACCESSION   DASSGX010000337 DASSGX010000000
VERSION     DASSGX010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149228"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="VFI12_05970"
     CDS             <1..1122
                     /locus_tag="VFI12_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055990.1"
                     /translation="LVLLSLLATLYLLARRWAAPLPAALLAAAALFVSEVNLSMRPQI
                     ISFLLIAITTAAWLRTREDQSIRWWLIPLTWLWAMTHGMWPIGIGIGFVAVAGLAMDR
                     ALPRHILARASLVPFLSAVGAALTPTGPALYGAVLAVNDRKQYFSEWASPNFSNPGCV
                     VLGILLALGATLMIRRTDRTVLSLGLFLVAAMCAVWSWRTVPVSAMILVPLVARQIPA
                     GTLANRRERFLIAGVSALALAALAVAVPRTASDPPSQPAWLDPALSALPAGTKVVDSS
                     DYGGYLMWRYPQLDLLSHGYGDTFTVAELQRNVNIATLAPGWDRELRMTGCTVAILRP
                     TSTLGYALIHEEHWIVLHRSATVAELRAPAGWLTTDEHE"
     gene            1260..>2302
                     /locus_tag="VFI12_05975"
     CDS             1260..>2302
                     /locus_tag="VFI12_05975"
                     /inference="COORDINATES: protein motif:HMM:NF021436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HET7055991.1"
                     /translation="MPPFVGLDEDQHAKRAAAVAHGDFNPRHRLAGSYGGVVEVPADL
                     VLAAEPVCETRGVTDQFNCRPITDLRNGMVTVTSTASAYNPIFYAMVGLAGRPFNGTQ
                     SLYAMRACAEILCALMIALAAAITSRWASTRAPLVALQLAAAPVLWYSASILAPNGLE
                     MCCALVMWAALLGIVRRQGDADRLTVVAGTVAACLLAAVRGLGPFWLVLIAMAVLVIT
                     PHETLRAVKAARWARTSALAIAACVAYAVAWTLAASATSVTPPVANPGSSIAAAFRGL
                     PLWALGTFAMFPGRTETAPVAVYTIGVAVWILFLAIAIGRARTTIRISAAFIVLAAAL
                     IPFALTVVTYHRIG"
BASE COUNT          355 a          778 c          712 g          457 t
ORIGIN      
        1 ctcgtattgc tgtcgctact tgcgaccctg tacctacttg ctcgacggtg ggcggcgccg
       61 ctcccggccg cgctgcttgc agcggccgcc ctcttcgtct cggaagtgaa cctttcgatg
      121 cggccacaga tcatcagttt cctattgata gcgatcacga ccgctgcctg gctgcggacc
      181 cgcgaagacc aatcgatccg atggtggctc atcccactga cctggctctg ggcgatgacc
      241 cacgggatgt ggccgatcgg tatcggcatc ggtttcgtag cagtcgcggg tctcgccatg
      301 gatcgagcac ttccacgaca catactggcc cgcgcgtcgc tcgtgccttt cctctcggcg
      361 gtgggggccg cactgacccc gaccgggccg gccttgtacg gtgccgtcct cgctgtcaac
      421 gatcgcaagc agtacttctc cgagtgggcc tcgcccaact tctcaaatcc cggatgcgtc
      481 gtcctcggaa tcctgctcgc tctgggcgcc accttgatga ttcgccgcac ggaccgcacc
      541 gtactgagct tggggctatt cctggttgct gcaatgtgtg cggtgtggtc gtggcgtacc
      601 gttccagtgt cagcgatgat cttggttccg ctggttgccc gccagattcc cgccggcaca
      661 ctggctaaca ggagggagcg gttcctcatc gctggggtta gcgcgctcgc cctggccgcc
      721 ctcgcggttg ctgtccccag gaccgccagc gatccgccga gtcaacccgc gtggctcgac
      781 cccgcgttgt cagcgcttcc ggcggggacc aaggtcgtcg actcgtcgga ctatggcgga
      841 tacctgatgt ggcggtaccc gcaattggac ctgctttcac acggctacgg ggatacgttc
      901 accgtcgccg agctgcagcg caacgtgaac atcgcaacgc tcgcgccagg gtgggatcgc
      961 gagcttcgta tgacaggctg cacagtggcg atcctccggc cgaccagcac gctgggctac
     1021 gcgctgatcc acgaggagca ctggatcgtg ctccaccggt ccgccaccgt cgccgagctg
     1081 cgtgcaccag cgggttggct cactacggac gagcacgaat gacgacagca ccgacccagc
     1141 caattcctgc ggttccggac ggcctgggtg cgagtcagcg ccgacgtgct cgcgcacggc
     1201 acatcttcct actgacgctg ctcggagcac tgttaatgga ggccgcttgg agtctcagcg
     1261 tgccgccatt cgtcgggctg gacgaggacc aacacgccaa acgggcggcc gccgtcgccc
     1321 atggcgactt caatccccgg caccgactgg cgggctcgta cggcggcgtc gtcgaggtgc
     1381 ccgcggatct ggtactcgcc gcagaaccag tgtgcgagac acgcggcgtc acagaccagt
     1441 tcaactgccg cccgatcacg gacctgcgga atggcatggt caccgtgacc agcaccgctt
     1501 ctgcgtacaa cccaatattc tatgcaatgg tcggactagc gggcaggcct ttcaacggga
     1561 cgcaatccct gtacgccatg cgagcgtgcg ccgagattct ctgcgcgcta atgatcgcac
     1621 tggcggcggc gatcaccagc cgctgggcca gcacacgggc tccgttagtc gcactgcagc
     1681 tcgcggcagc acctgtgctc tggtactccg cgagcattct ggctcccaat ggactggaga
     1741 tgtgctgcgc cctcgtcatg tgggccgcac tgctaggcat cgtccgtcgt cagggggacg
     1801 ctgatcgcct cactgtggtt gcgggaacag tggcggcgtg tctcctagcg gcggttcgcg
     1861 gattgggacc attctggctg gtcctgatag cgatggctgt tctggtcatc acgcctcacg
     1921 agacgctccg tgcagtcaag gccgcgcggt gggcaaggac gagcgctctg gccatcgctg
     1981 catgtgtcgc gtacgccgtc gcctggacgc ttgccgcctc ggcgacttct gtcacgcctc
     2041 ccgtcgcgaa ccctggatct tcgatcgcgg ccgctttcag gggcctgcca ctctgggcac
     2101 tcggcacatt cgccatgttc ccgggacgca cggaaacggc cccggtcgcc gtttacacaa
     2161 tcggagttgc tgtctggatc ctgtttcttg ccatcgccat cggccgtgcc cggaccacca
     2221 tccggataag cgccgcattc attgtgctgg ctgcggcact catcccattc gcactcacgg
     2281 tcgtgacgta tcatcggatc gg
//
LOCUS       DASSGX010000338         2271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_167654, whole genome shotgun sequence.
ACCESSION   DASSGX010000338 DASSGX010000000
VERSION     DASSGX010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167654"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="VFI12_05980"
     CDS             complement(<1..1003)
                     /locus_tag="VFI12_05980"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HET7055992.1"
                     /translation="MKSRLSSIRRLRSAFALFTAAVLMTTVSFLDGPAAQAAVPSGFT
                     DNVVTSVSQPTDIAFTPTGTMLVTSQAGRLYKWDGGTRTQLLDLSGKICSDFERGLLG
                     VTVDPAFASNNYIYLFYTFNKNNTCVDNSPSSPVNRVSRFVFNGTSVSGETVLVDNMP
                     SPNGNHNAGGLVFGKDGYLYISIGDGGCEIGSPGECGGDNDNARVINRLQGKILRVTR
                     DGKAPTSNPFYSQGGECRISGSTTAGMHCRETYAWGFRNPFRIAADPNGTTTRIFAND
                     VGQNLWEEVDHVLAGKDYGWNNCEGFHANSSYSTCGFADQDPIFEYGHGSCNSLSGGA
                     "
     gene            complement(1104..1349)
                     /locus_tag="VFI12_05985"
     CDS             complement(1104..1349)
                     /locus_tag="VFI12_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055993.1"
                     /translation="MTDQDPKAQLGYRLYWKRIALHLNLGDIAKELGTRSVSLSKFEK
                     GDFTQLTREQLLGYLETLDMANLAENYYALMPEPLEA"
     gene            complement(1384..1464)
                     /locus_tag="VFI12_05990"
     tRNA            complement(1384..1464)
                     /locus_tag="VFI12_05990"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1429..1431),aa:Leu,seq:caa)
     gene            1559..>2271
                     /locus_tag="VFI12_05995"
     CDS             1559..>2271
                     /locus_tag="VFI12_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET7055994.1"
                     /translation="MATTIASLKEYSLLIDGKSVASASGKTFDTVSPTTNQPIGRVPL
                     AGIEDVERAIQAARTAFDDGPWPRWTPLERSRALHRVSHILRDRIDEISTLETMNCGK
                     IIVESRADVTASANCFEYYGNLATQLWGEQIPMNGPLLDYTLREPVGVCAQIVPWNFP
                     LLMAAWKLAPALAAGNTRSLKPASATPLTAIVLGEICRDAGIPNGVVNVITGPGGSIG
                     EAMVTSPVVDKVAFTGETST"
BASE COUNT          414 a          689 c          741 g          427 t
ORIGIN      
        1 aggcgccgcc cgacaaggag ttgcaggacc catggccgta ctcaaagatc gggtcctgat
       61 cggcaaatcc gcaggtggag tatgagctgt tagcatggaa accttcgcag ttgttccagc
      121 cgtaatcctt gccggcaagt acgtgatcga cctcctccca gaggttctgc ccgacgtcgt
      181 tggcgaagat ccgtgtcgtc gttccgttcg gatcggcggc aatccggaat ggattgcgaa
      241 agccccaggc gtaggtctcg cggcaatgca tgccagccgt cgtgctgcca ctgatccggc
      301 actcgccgcc ctgggaatag aacgggttcg acgtcggagc cttaccgtcc cgggtcacgc
      361 ggaggatctt gccctggagg cggttgatga cgcgggcatt gtcgttatcg ccgccgcact
      421 ctcccggtga gccaatctca cagccgccat cgccgattga gatgtagagg tagccgtcct
      481 tgccgaagac gaggccgccg gcgttgtggt tgccgttcgg ggagggcatg ttgtcgacca
      541 gcaccgtctc gcccgaaacg gaggtgccat tgaagacgaa acgcgagacg cggttcaccg
      601 gcgagctcgg gctgttgtcc acgcatgtgt tgttcttgtt gaaggtatag aaaagataga
      661 tgtagttgtt cgaggcaaat gcagggtcga ccgtgacgcc gagcaggcca cgctcgaagt
      721 cggagcagat cttgcccgag agatcgagga gctgcgtcct cgtaccgccg tcccacttgt
      781 aaagccggcc agcctggctt gtgaccaaca tcgtgccggt cggcgtgaag gcgatgtcgg
      841 tcggctggct gaccgatgta accacgttgt cggtgaaacc gctggggacg gcagcctgcg
      901 cggcggggcc gtccaggaac gagaccgtcg tcatcaggac agcagcggtg aacagcgcga
      961 acgcgctgcg caatcgacga atcgacgaga gtcggctctt catacggctc cccggagctc
     1021 accgcatatc gcaatgcagg tgcgattgtg tcgttttgag gagtctacgg gttcagcgag
     1081 cgcgcgtcac tatgccgggt gggctacgcc tcgagtggtt ccggcatcag ggcgtagtag
     1141 ttttccgcca ggttcgccat atccagcgtt tcgaggtacc cgaggagctg ctcgcgggtg
     1201 agctgcgtga aatcgccctt ttcgaacttg ctcaaagaga ccgacctggt cccgagctct
     1261 ttggcgatgt cgccgaggtt aaggtgaagc gcaatccgct tccaatacag ccgatagcca
     1321 agttgcgcct ttggatcctg gtcggtcatg cgggtaggtc tccggaatcg ccattgaatt
     1381 cggtaccggg agagggattc gaaccctcac gggttgcccc ggcggatttt gagtccgctg
     1441 cgtctgccat ttcgccaccc cggcaggtga tcgaagggaa attgtagcgg atgaggccgg
     1501 ccggagggga gtatcctacc ggtatgggtt cctgattcat cgctaaccgg agacgcctgt
     1561 ggccaccaca atcgcatcgc tcaaggaata ctcgctgctg atcgacggca agtccgtcgc
     1621 gagcgccagc ggcaagacgt tcgacaccgt cagcccgacc acgaaccagc cgattggccg
     1681 cgttccgctc gcgggcattg aggatgtcga gcgcgccatc caggccgccc gaacggcctt
     1741 cgatgacggc ccatggccgc gctggacgcc gctcgagagg tcgcgggccc tccatcgggt
     1801 gtcgcacatt ctgcgggacc ggatcgacga gatctcgacc ctcgagacga tgaactgcgg
     1861 caagatcatc gtcgaatccc gcgccgatgt gaccgcctcg gccaactgct tcgagtatta
     1921 cgggaacctc gcgacgcagc tgtggggcga gcagatcccg atgaacgggc cgctgctcga
     1981 ctacaccctg cgcgagccgg tcggcgtctg cgcccagatc gtgccctgga acttcccgct
     2041 cctgatggct gcctggaagc tcgccccggc gttggcggcg ggcaacacgc ggagcctcaa
     2101 gccggcctcg gccacgccgt tgaccgcgat cgtgctcggc gagatttgtc gcgacgcggg
     2161 cattccgaac ggcgttgtca acgtgattac cggccccggc ggatcgatcg gcgaggcgat
     2221 ggtgacgagc ccggtcgtcg acaaggtcgc cttcacgggc gagacctcga c
//
LOCUS       DASSGX010000339         2155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_932466, whole genome shotgun sequence.
ACCESSION   DASSGX010000339 DASSGX010000000
VERSION     DASSGX010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932466"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..614)
                     /locus_tag="VFI12_06000"
     CDS             complement(96..614)
                     /locus_tag="VFI12_06000"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HET7055995.1"
                     /translation="MSPSQALWRTAEPAQTKITLTGEGERALRAKLTELRRELEEIYP
                     ARLREARSFGSAGGNDDYLQIKEEEALLALRVTRLQAMLDGATVLRSSDAEAGVVAIG
                     HRVEVEDRDSGEVSGYDLVGGFERVVNGVSADSPVGRALIGRRVGDRTEVELPGGRVV
                     RLRILGCRLSEG"
     gene            complement(714..1166)
                     /locus_tag="VFI12_06005"
     CDS             complement(714..1166)
                     /locus_tag="VFI12_06005"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HET7055996.1"
                     /translation="MKRSAALTLLSHDHHHALVVAQRLRRAEDRSQATRELLDFWESQ
                     GREHFEVEEEVLVPTWLELDPDADRALAERLADEHLVIRSLVRAAQSGALDLDGLAET
                     GRLLADHVRFEERDLFPLIEGALRSGALATLGDEIGDRRTGSAPGRSS"
     gene            complement(1163..>2155)
                     /locus_tag="VFI12_06010"
     CDS             complement(1163..>2155)
                     /locus_tag="VFI12_06010"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HET7055997.1"
                     /translation="NFFQAAAGGDVISYQHIFWFYSHPAVYIMMLPGFGIVSEVISTH
                     ARKPIFGYRLMAISLLGIVVLSFSVWAHHMFVSGMFAWLRVPMMLTSALIAIPTGIKI
                     FSWVGTLFNGKIHLESTGMLFALGFVGSFILGGISGVMTAMVPLDIHVTDTYFIVAHI
                     HFVLFAGSVFTIFAGIYHWFPKMTGRMYDERLGHWHFWLTLLGTWGTFVPMHWVGMEG
                     MPRRVADYAAQFGDWNLFISICGFCLGASQLIFLYNMIVSWRWGPRAPANPWGAKSIE
                     WLVSSPPPKFNFPTIPRVVGGPYEFGVKGARHAVFDPVGEPRKAPDQVAAAPGK"
BASE COUNT          374 a          728 c          732 g          321 t
ORIGIN      
        1 gaccggcaag atcgatcgga ccggcctgaa gcggatggcc gaggaccacc tccacccgca
       61 cgggctcgac tgaccggggc cagaccgggg ccggctcagc cctcgctgag ccggcagccg
      121 aggattcgca acctgaccac ccgaccgccc gggagctcga cctcggtccg gtcgccgacg
      181 cggcgcccga tcagcgcccg gccgaccggg gaatcggcgg agaccccgtt gacgacccgt
      241 tcgaagcctc cgaccaggtc gtagccgctg acctcacccg agtcacgatc ctcgacctcg
      301 acccgatgtc cgatcgcgac gacgcccgct tcggcatcgg agctgcgcag caccgtcgcc
      361 ccgtcgagca tcgcctgcag gcgggtgacc cgcagcgcca gcagcgcctc ctcctccttg
      421 atctggaggt agtcgtcgtt acccccggcg ctaccgaacg agcgcgcttc ccgcaggcgc
      481 gccggataga tctcttcgag ctcgcggcgc agctcggtca gcttggcccg cagggcacgt
      541 tcgccctcgc cggtcagcgt gatcttcgtc tgtgcgggtt ccgccgtgcg ccacagagcc
      601 tggctcgggc tcacggggtc ggtcgtcgct gtctgtgctg ttgtgcttgt gctcatgggg
      661 ccagtatcgg cgccggggac ccggcgttga atgggtccaa atgagcatct ttttcagctg
      721 cttcgccccg gtgccgatcc cgttcggcga tcgccgatct catcgccgag cgtcgccagg
      781 gcgccggatc tgagcgcccc ctcgatgagc ggaaagagat cgcgctcctc gaagcgcacg
      841 tggtcggcga gcaggcgccc ggtctcggcc agcccgtcca ggtcgagggc gcccgattgt
      901 gctgcgcgaa ccagcgagcg gatgaccagg tgctcgtcgg cgagccgctc tgcgagcgcc
      961 cgatcggcgt cggggtcgag ctcgagccag gtcggcacga gcacctcctc ctcgacctcg
     1021 aagtgctcgc ggccctgcga ctcccagaag tcgagcagct cccgcgtggc ctgcgatcga
     1081 tcctcggccc gtcgcaatcg ctgggcgacg acgagcgcgt gatgatgatc gtgggaaagc
     1141 agcgtcagcg cggcgctgcg cttcatttcc ctggggcggc cgccacctgg tcgggcgcct
     1201 tacgcggctc gccgaccggg tcgaagacgg cgtggcgcgc cccctttacg ccgaactcgt
     1261 aggggccgcc gacgacccgc ggaatggtcg ggaagttgaa cttcggcggc ggcgaggaga
     1321 ccagccactc gatcgacttg gcgccccacg ggttcgccgg agcccgcggc ccccaacgcc
     1381 agctcacgat catgttgtag aggaagatca gctgggaggc cccgagacag aagccgcaga
     1441 tcgagatgaa caggttccag tcgccgaact gggcggcgta gtcggccacc cggcggggca
     1501 tcccctccat cccgacccag tgcatcggca cgaaggtccc ccaggtgccg agcagggtga
     1561 gccagaaatg ccaatggccg aggcgctcgt cgtacatccg cccggtcatc ttcgggaacc
     1621 agtgatagat cccggcgaag atcgtgaaga ccgagccggc gaagagcacg aagtggatgt
     1681 gggcgacgat gaaataggtg tcggtgacgt ggatgtccag cggcaccatg gccgtcatca
     1741 cgccgctgat cccgccgagg atgaagctgc cgacgaagcc gagcgcgaag agcatcccgg
     1801 tgctctcgag atggatcttg ccgttgaaca gggtgccgac ccaggagaag atcttgatgc
     1861 cggtggggat cgcgatcagc gccgaggtca gcatcatcgg cacgcgcagc caggcgaaca
     1921 tgccggagac gaacatgtgg tgcgcccaga cgctgaacga cagcacgacg atgccgagca
     1981 gcgagatcgc catcaaccgg taaccgaaga tcggcttgcg ggcgtgggtg gagatcacct
     2041 ccgagacgat cccgaagccc ggcaacatca tgatgtagac ggccgggtgg gagtagaacc
     2101 agaagatgtg ctggtagctg atcacgtcgc cgccggcggc cgcctggaag aagtt
//
LOCUS       DASSGX010000340         2236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1585723, whole genome shotgun sequence.
ACCESSION   DASSGX010000340 DASSGX010000000
VERSION     DASSGX010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1585723"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="VFI12_06015"
     CDS             complement(<1..649)
                     /locus_tag="VFI12_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7055998.1"
                     /translation="MRGILTIERGEVYDPQSLGERNLIAGGGKTLAVEAAAGSMDLPC
                     ERRIDAGGLRVIPGLIDQHLHLIGGGDGNGISGRVPELDARTILAGGTTTAVGVLGVE
                     TVTRGLLLLLRKTQELRAGGLSAFMYTAGMPLPAQNMLGTVFADVSLIDQVLGAKTAI
                     SESLHPNTDPHLLTALAGELMRARAMTGKAAVLHCHVGGLEAALEPLFALHEKLRM"
     gene            complement(857..1252)
                     /locus_tag="VFI12_06020"
     CDS             complement(857..1252)
                     /locus_tag="VFI12_06020"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HET7055999.1"
                     /translation="MPPRTWKAWLWASNSISCVCSGQWVAHPGNIIDYRIDITRPDDP
                     VMQGLSGFPYRSEQYYMHVDPSNEVLATTSFSGEHAWWIDGVVMPAVWKRRHGKGRVF
                     YSSLGHVSAEFEVPEMRTILRRGMNWAAR"
     gene            1188..1385
                     /locus_tag="VFI12_06025"
                     /pseudo
     CDS             1188..1385
                     /locus_tag="VFI12_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011255179.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1573..>2236
                     /locus_tag="VFI12_06030"
     CDS             1573..>2236
                     /locus_tag="VFI12_06030"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HET7056000.1"
                     /translation="MKTDFHVAENSDSVRSVTRALGLLTALGKEGRSLTELARAVGIS
                     PSTASRLLNTLQGQGFIANTEENKYVPGLALTSLLYSTDQWAPLRQVARAATAALRAE
                     LNETVAFFVRNAGDRLCIESSESAQVVRRICRPGERKTIFRGAAGKALLAFGEDKDPW
                     ADLFGTDDRFKMATDSVRTVAELKTECDEIRRQGYAFSRQESTLESWAVAAPVYIDGK
                     LVG"
BASE COUNT          427 a          697 c          699 g          413 t
ORIGIN      
        1 gcatacgaag cttttcatgg agggcaaaga gcggctccag agccgcctcc agcccgccga
       61 catggcagtg gagcaccgcc gccttgccgg tcatcgcccg ggcacgcatg agttcgcccg
      121 caagcgcggt gagcaggtgc ggatcggtgt tcggatggag gctttccgaa atcgcagtct
      181 ttgcgcccag cacctgatcg atcagcgaaa cgtcggcaaa aacggtgcca agcatgttct
      241 gtgccgggag cggcattccg gccgtgtaca tgaacgcgga aaggccgccg gccctcaatt
      301 cctgggtctt gcgcagcagg agcagcagcc cccgcgtgac ggtctccacg ccgagcaccc
      361 cgacggcggt cgtggtccct cccgcaagga tggtccgcgc gtcaagttcc ggcacccggc
      421 cggaaattcc gtttccgtcg ccgccaccga tcaggtggag atgctggtcg atgaggccgg
      481 ggatcacacg caggccgcca gcatcgatgc gccgctcgca aggcaggtcc atacttcccg
      541 cagccgcttc gacggccagc gtcttgccgc ctcctgcgat gaggttccgt tctcctagag
      601 actgcggatc gtaaacctcg ccgcgttcga tcgtcagaat gccgcgcatc gttgcgtgaa
      661 tgccctacat gctttccgaa ccgtggtcgc acaatggcag ccatcggtgc gatctcgaaa
      721 ccatcattcg caagccagag ccgtatgtca ataattagaa ttttcgtcct atatattggg
      781 acagtccatt gcccgggccg gtatctggct tgggtcacaa tctgccgcgc ctgggtcgcg
      841 aatcatttgc ggtcggtcag cgcgcggccc agttcatgcc gcgccggagg atggtgcgca
      901 tctcgggcac ctcgaactcc gccgacacgt ggccgagcga ggagtagaag acgcgcccct
      961 tgccgtgccg ccttttccac acggcgggca tgacgacgcc gtcgatccac cacgcatgtt
     1021 cgcccgagaa gctcgtggtc gccagcacct cgttggaggg gtcgacatgc atgtaatatt
     1081 gctccgagcg atagggaaac ccggacagcc cctgcatcac ggggtcatcg ggacgggtga
     1141 tgtcgatgcg atagtcgatg atgttgccgg gatgggcgac ccactgtccg ctgcagacgc
     1201 acgagatgct gttcgatgcc cacaaccacg ccttccaagt tctgggcggc atccctggcg
     1261 cgggatctac gacaacatgc gtacggcggt cgacctgatt gatcgcggca aagatcgcta
     1321 ggtcaacgcc cgcttctcgg ccatggtcag ccatcacctg tttcgaggcc gagatctgca
     1381 acccaatact ttagggcttc tttgcccctt aaaagctcac ggcgggttcg aaacagggtc
     1441 gttcggcccc ggactcggaa tttgcaaaaa tgccggcggc taagcagggc tgttcgttca
     1501 taagagccgg agaacggcat tttccgtcgt gacaattgat attttgttca atcgcagtat
     1561 aaattccgaa caatgaaaac cgatttccac gtagcggaaa attctgacag cgtgcgctcg
     1621 gtcacccggg cgctcggtct gttgaccgcg ctcggcaagg aaggacgctc gctcaccgag
     1681 ctcgcgcggg ccgtcggtat cagcccgagc acggcatcgc ggttgttgaa cacgctgcag
     1741 gggcagggct ttatcgccaa taccgaggag aacaaatacg tgcccggcct ggccctgacc
     1801 tcgttgctct acagcacgga tcagtgggcg ccgttgcgtc aggtggcgcg ggccgcgacc
     1861 gcggcgctgc gcgccgagct gaacgaaacc gtggccttct tcgtccgcaa cgcaggcgac
     1921 cgtctgtgca tcgaatcctc tgagtccgca caggtggtgc ggcgtatctg tcgccccggc
     1981 gaacgcaaga cgattttccg cggagcggcg ggcaaggcgc tgctggcctt cggcgaagac
     2041 aaggatccat gggccgacct gttcggtacg gacgaccggt tcaagatggc gaccgacagc
     2101 gtgaggacgg tcgcggaact gaagacagaa tgcgacgaga tccgccggca gggctacgcc
     2161 ttcagccggc aggaatcgac gctggaatca tgggccgtcg cggcgcccgt ctatatcgac
     2221 ggcaagctgg tcggcg
//
LOCUS       DASSGX010000341         3413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_277442, whole genome shotgun sequence.
ACCESSION   DASSGX010000341 DASSGX010000000
VERSION     DASSGX010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277442"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..1924
                     /locus_tag="VFI12_06035"
     CDS             293..1924
                     /locus_tag="VFI12_06035"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET7056001.1"
                     /translation="MVDALERGRTHFDERHWGAAHAELTHADRDAPLDIPDLERLAVA
                     AYLIGHDAESVAAWSRAYRGHLDSDNLQAAARCAFWSAFGLMARGEYAQGSGWLARAR
                     RHVEGFDKDCPERGMVLVPGAIQQLEEGDTPGAFAAFTEIGAIGARHGIADLVATSNL
                     GCGHALIRFGQVADGVSLLDEAMVCVTSDEVSPVLVGVIYCGVIEVCHEIYDLQRARQ
                     WTEALSRWCESQPDLVPYRGDCLVHRAEIMQIHGSWQDALAQAIEACELLSDPPGQPA
                     IGHAFYQRAEIHRLRGEVRDAEEAYRQASEWGWAAQPGMALLRLAQGHSDAAEAAIRR
                     ETLEIRDHSRRCRLLPAYIEVMLGVGDVESARTAADELSKIAGEIDVAPVRAASDQAL
                     GAVLLAEGDPAQALETLRRAWKTWHELGIPYEAARVRVLIGQCCREVGDEETARLELH
                     AALGTFRRLGARPDIARAEALARKASPGPAGDLTAREVEVIRLVAAGKTNRMIAAELF
                     LSEKTVARHVSNIFTKLGISSRSAATAYAFEHRLV"
     gene            2049..2480
                     /locus_tag="VFI12_06040"
     CDS             2049..2480
                     /locus_tag="VFI12_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056002.1"
                     /translation="MTARTETARPSGVEIDLLSRRFAETFETLTASRDVFADDAFFDL
                     NMPIWRFQLIGPEAFSDQLKHINQGPISIDVLRTVPSDSGFTTEHEEHQMVDGEDLTA
                     RRLWLCEVRDGLITAAIGYCSGEWDAELRARHAIEAPMVRP"
     gene            2545..>3413
                     /locus_tag="VFI12_06045"
     CDS             2545..>3413
                     /locus_tag="VFI12_06045"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET7056003.1"
                     /translation="MITGTHTITSIEGSQVRLDGAQVDVLERRIAGRIITEGQPGWDD
                     AVLIWNAMWPARPALVAQPRSARDVAAIVSFARENGLLVSIKGGGHNIAGTAIAEGGL
                     MIDMAAMRDVVVDPAARLVHAGGGCLLGDVDRATQQYGLATVLGLVSDVGVAGLTLGG
                     GFGNLARRFGWAVDNLESVEVVTADGQIRVASANEHPDLFWAIRGGGGNYGVVTRFTF
                     RLHDVGPNVFGGMLVWGVDRARDVFDAYRDVTDRGPRELMTGMIVSTAPPAPFVPVEW
                     HGKRIVAVLVCHSG"
BASE COUNT          599 a         1044 c         1165 g          605 t
ORIGIN      
        1 atcaacacct tcatgctgga gcgctccgcc tacctgatga agttcgtgaa cgacctttcg
       61 aagatcaacg gcgggcgcgt cttcttcgcg agcccggacc acctcggcga atacgttctg
      121 gtcgactacg ttcagaacaa gcggaagtgg atccagtaac gtcgtacggc aactgaacga
      181 atccaattgt ccgacctgcg agcctccccg gcccctcccg gcgggtgtag agtgcctgtg
      241 caacccgaga ctgtgtccag atcaattagc ctctggccgg gaacatacgc acatggtcga
      301 tgcactcgaa cggggccgaa cccacttcga tgaacgccac tggggcgccg cgcacgccga
      361 gttgacccat gccgaccggg acgcgccgct ggacattccc gatctcgagc ggcttgcggt
      421 cgccgcctac ctgatcgggc acgacgcgga aagtgttgcc gcctggtcgc gcgcgtaccg
      481 cggacacctg gacagtgaca acctgcaagc ggcggcgcgt tgtgcgttct ggtcggcgtt
      541 cggactcatg gcccgcggcg aatatgcgca gggcagcgga tggctggcac gagcccggcg
      601 gcacgtagag ggattcgaca aggattgccc ggagcgcggg atggtcctgg tgccgggcgc
      661 gatccagcag cttgaagagg gcgacacccc gggcgctttc gccgcgttca ccgaaattgg
      721 ggcaatcggc gcgcgtcatg gcatcgccga cctggtcgcc acgagcaacc tcggctgcgg
      781 ccatgccctg atccgcttcg gccaggttgc ggatggtgtt tcgctcctcg acgaggcaat
      841 ggtctgcgta acgagtgacg aggtttcgcc ggtcctggtc ggggtgatct actgcggcgt
      901 gatcgaggtg tgccacgaga tctacgattt acagcgggcg cggcagtgga ccgaggccct
      961 gagccgctgg tgcgaatcgc aacctgatct cgttccatat cgcggagatt gccttgtcca
     1021 ccgggccgag atcatgcaga tccacggttc ctggcaggat gcgctggcgc aggcaattga
     1081 ggcgtgcgag ctgttgtcgg atccacccgg acaaccggct attggtcatg cgttctacca
     1141 gcgcgcggag atacaccggc ttcggggcga agttcgggat gcggaagagg cgtatcggca
     1201 agccagtgag tggggttggg ccgcgcagcc gggcatggcg ctcttgcggc ttgcccaggg
     1261 tcattccgat gcagcggagg cggccatccg gcgcgagacg ctcgaaatcc gggaccattc
     1321 ccgcaggtgt cgcctgctgc ctgcttacat agaggtgatg cttggcgtgg gtgatgtcga
     1381 atcggcccgc accgccgccg acgaactttc taagattgcg ggagagatcg acgtggcgcc
     1441 tgtgcgagcg gcgtcggacc aggccctcgg cgcggtattg ctcgccgagg gcgatcccgc
     1501 gcaggcgctt gagaccttgc ggagagcctg gaaaacctgg cacgagcttg gcattccgta
     1561 cgaagccgcc cgagttcgcg tgctgattgg ccaatgttgc cgggaggtcg gcgatgagga
     1621 gaccgcccgc ctggagcttc acgctgcgct gggaacattt cggcgcctcg gagcgcggcc
     1681 ggacattgcc cgtgctgaag ccctggcccg aaaggcatcg cctggaccag cgggcgatct
     1741 gacggcgcga gaggtagagg tcatccgcct cgtcgctgcc ggaaagacga accgcatgat
     1801 cgccgccgaa ctcttcctca gcgagaagac cgtcgcccgg cacgtcagca acatcttcac
     1861 caagctcggc atttcgtcgc gatccgccgc gaccgcctat gccttcgagc atcgcctcgt
     1921 gtaacgccct acacagaaat acccataccg gcgcatccgg cgaattgggc ataacgcccg
     1981 acgcgaatac agcagcgtct ccctagagtc gttcctgtcg acaggagtga atgaaaggga
     2041 gaccagctat gacagcacgc accgaaacgg cgagaccgag cggcgttgag atcgatctgc
     2101 tcagccgccg ttttgcggag acgttcgaga cgctcacggc gagccgggat gtctttgcgg
     2161 atgacgcctt tttcgacctc aacatgccga tttggcgatt ccagctcatt gggccggagg
     2221 cattctcgga ccaactcaag cacatcaatc aggggccgat ctccattgac gtgctgcgga
     2281 cggtgccgtc cgattccggc ttcacgactg agcatgagga gcaccagatg gtcgacggcg
     2341 aggacctgac cgcgcgccgg ctctggctgt gcgaggtccg ggacgggctc atcacggcgg
     2401 caatcgggta ttgcagcggt gagtgggacg ccgagctgcg cgcccgccac gcgatcgaag
     2461 cgcccatggt gcggccgtga cagttctatg gaatgagata tacgcatgcg ccgtcaaatc
     2521 gacggctgcg caaggagcca ggcgatgatt acaggaacgc acactattac cagtatcgaa
     2581 ggcagccagg ttcggctcga tggagcccag gttgatgtgc tcgaacgacg catcgcgggg
     2641 cgcatcatca ccgaaggtca acccggctgg gatgacgccg tgttgatctg gaatgcgatg
     2701 tggccggcac ggcccgcgct ggtggcgcaa ccgaggtctg cccgggacgt cgcggcgatc
     2761 gtctcgttcg cgcgcgaaaa tgggctgctg gtcagcatca agggcggcgg ccacaatatc
     2821 gcgggcacgg cgatcgcaga gggcgggctg atgatcgaca tggccgcgat gcgggatgtc
     2881 gtcgtcgatc cggcggctcg tctcgttcat gccggcggcg ggtgcctgct cggggacgtc
     2941 gatcgggcaa cgcaacagta cggcctggcg accgtccttg gattggtctc ggacgtcggt
     3001 gttgccgggc tcaccctcgg cggtgggttc gggaaccttg cgcgccggtt cggttgggcg
     3061 gtcgacaacc tcgagtcggt cgaggtcgtc acggcggacg gccagattcg cgtggccagc
     3121 gcgaacgagc atccggacct gttctgggcg attcgcggcg gcggcggaaa ctacggggtc
     3181 gtgacccggt tcaccttccg gctccatgat gtgggaccga atgtcttcgg cggcatgctg
     3241 gtgtggggtg tcgaccgggc ccgggacgtc ttcgacgcct atcgcgacgt gaccgatcgt
     3301 ggcccacgtg agctgatgac cggaatgatc gtgtccaccg cgccgcccgc gccgttcgta
     3361 cccgtcgagt ggcacgggaa gcgcatcgtg gcggtgctgg tgtgccactc cgg
//
LOCUS       DASSGX010000342         2698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_822241, whole genome shotgun sequence.
ACCESSION   DASSGX010000342 DASSGX010000000
VERSION     DASSGX010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822241"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="VFI12_06050"
     CDS             <1..287
                     /locus_tag="VFI12_06050"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7056004.1"
                     /translation="QVGEEIGQEMHPDVDQFFRVEHGQADAILESEIHKLVDGSVLII
                     PAGTEHNVVNTSPNEPLRLYTIYSPPNHPDGTINKTKAEAEEYERIHHGH"
     gene            372..1973
                     /locus_tag="VFI12_06055"
     CDS             372..1973
                     /locus_tag="VFI12_06055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HET7056005.1"
                     /translation="MLQHRVNQKVAVSVVYICAMLMNSLDSTVVNVALPTLSRQFHVD
                     SAAIESIIVGYLVSLAVFIPASGWLGDRFGHKRILLIALALFVTASALCGAAQSLDQL
                     VLFRILQGAGGGLLTPVGMALLFRTFPPEERVGVARVLMFATIIGPAAGPILGGFMIE
                     HFSWRWIFYINLPVGISALLFGLLCLEDHPERQEDPFDIPGFLLAGSGFAFAMYALSE
                     GSRSGWTAPVIVISGLIGIALLAAFVFVELRTEKPMVQLRVLANRLFRRTMTVSLFAT
                     AAFLGTLFAVPLFLQDGIGVSPLTSGLTVFPEAIGVVVSTQLVARIYPRIGPSRLMSG
                     GLVLVAAVLGGLSLLDFGVDLWLFRALMFGLGAGMACIFVSNQAAALADISREMTGRA
                     SMLYSVQRQIGAACGVALLSTIMLSVGARRTLPGGETTPDITAYRAAFLVAAGLALIG
                     AALATRVPDEDAEASMRKPVKRRAGEEPSRRAANVSREPSDVGLQSSSKGQGAGNEVG
                     AVRHLPGTARHTIPTAEPTDRRHPV"
     gene            complement(2040..2402)
                     /locus_tag="VFI12_06060"
     CDS             complement(2040..2402)
                     /locus_tag="VFI12_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056006.1"
                     /translation="MYAKLIVWRLGAEYRDGPGYERFLRNFAPASLQKLRQYGMLDGF
                     IVRLTHNTVLTVNLYEMADGAYSAWHNVVSREDYAAAGDLEMLFHLISRGDDLPMVGE
                     MVAGADDQHKDDANNIGE"
     gene            complement(2512..>2698)
                     /locus_tag="VFI12_06065"
     CDS             complement(2512..>2698)
                     /locus_tag="VFI12_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056007.1"
                     /translation="PFPDVRDPELRAQYAAVTVLCSCGEESHATVSGFDEADVRCACG
                     RGWGVRLALNPIVSQSE"
BASE COUNT          443 a          919 c          843 g          493 t
ORIGIN      
        1 tgcaggtcgg cgaggaaatc gggcaggaga tgcatccgga cgtcgaccag ttcttccgag
       61 tcgagcacgg tcaagccgat gcgatcctcg agagtgagat acacaagctc gtggacggtt
      121 ccgtcctgat tatcccggcc ggcaccgagc acaacgtcgt caacacctcg cctaatgagc
      181 cgctccggct gtacacgatc tattcgccac cgaatcaccc tgacggaacg atcaacaaga
      241 ccaaagccga ggcggaagaa tacgagcgaa tccaccacgg tcactagccg ctgaagcccg
      301 ccttccgtcg tcttccgccc cgccccggta tccttcccgg gcgcgggtcg cgcggatggc
      361 gggtgaaatc agtgctgcag catcgggtca atcagaaggt cgcggtctcc gtcgtctaca
      421 tctgcgcaat gctgatgaac tcgctcgact cgaccgtcgt caatgtcgcc ctcccgaccc
      481 tcagtcgcca gttccatgtc gattccgcgg cgatcgagtc catcatcgtc ggctatctgg
      541 tctcgctcgc cgtcttcatc ccggcatcgg gctggctcgg cgaccgcttc gggcacaagc
      601 ggatcctgct gatcgccctc gccttgttcg tgaccgcctc ggcgctctgt ggcgccgccc
      661 agagcctcga ccaactcgtc ctgttccgga tcctgcaggg agccggcggc ggattgttga
      721 cccccgtcgg gatggccctg ctcttccgca ccttcccgcc cgaggagcgc gtcggcgtgg
      781 cgcgggtgct gatgttcgcg acgatcatcg ggccggccgc gggacccatc cttggcggct
      841 tcatgatcga gcacttctcg tggcgctgga tcttctacat caacttgccg gtcggcatct
      901 ccgcgctgct tttcggcctg ctctgcctgg aggaccatcc ggagcggcag gaggacccgt
      961 tcgatatccc tggcttcctc ctggccggca gcggtttcgc ttttgccatg tacgccctca
     1021 gcgaaggttc gcgcagtggc tggaccgcgc cagtgatcgt catctccggg ctgatcggga
     1081 tcgcgctgct ggccgcattc gtcttcgtcg agctgcggac agagaaaccg atggtccagc
     1141 tccgggtact ggccaaccgc ctcttccggc ggacgatgac ggtctcgctg ttcgcgacgg
     1201 ccgcgttcct cgggacgctc ttcgccgtgc cactcttcct gcaagacggc atcggtgtct
     1261 ccccgctgac gtctggcctg accgtcttcc cggaggcgat cggcgtggtc gtctcgacgc
     1321 agctcgtcgc gcggatctac ccgcggatcg ggccgagcag gctgatgagc ggcggcctgg
     1381 tcctggtcgc ggcggtgctg ggcgggctga gtctgctcga tttcggcgtc gacctctggc
     1441 tgttccgggc gctgatgttc gggctgggcg cgggcatggc ctgcatcttc gtttcgaacc
     1501 aggcggccgc gttggccgac atctcccgcg agatgaccgg ccgggcctcg atgctctaca
     1561 gcgtccagcg gcagatcggc gcggcctgcg gtgtggcgtt gctgagcacg atcatgctct
     1621 cggtcggcgc ccggcggacg ttgcccggtg gcgagacgac cccggacatc acggcctacc
     1681 gcgccgcctt cctcgttgcc gccggcctcg ccctgatcgg cgcggcgctg gcaacccggg
     1741 tgccggacga ggacgcggaa gcaagcatgc gcaagccggt gaaacggcga gcgggtgagg
     1801 agccgagcag acgagcagcg aacgtaagtc gtgagccatc ggacgtcgga cttcagagct
     1861 caagcaaggg gcagggcgca ggcaacgagg taggggcagt tcgccatctg cccggcacag
     1921 cccggcacac gatacccact gccgagccca cagatcgccg ccaccccgtg taggggcgct
     1981 tttcaatgca cctgcgcaga cgaacccgcg cgtcaccatg gttcccgatc actgacctgt
     2041 tactccccga tgttgttggc gtcgtccttg tgctggtcat ccgctccagc gaccatctcg
     2101 ccaaccatag gcaagtcgtc accgcgactg atcaggtgaa acagcatctc cagatcgcca
     2161 gccgcggcgt agtcctcccg gctgaccacg ttgtgccacg ccgaatacgc gccatcagcc
     2221 atctcataca gattgacggt cagcacggtg ttgtgggtga ggcggacgat aaagccgtcg
     2281 agcatgccgt attggcggag cttttgaagc gaagcgggcg caaagttgcg caggaatcgc
     2341 tcgtaccctg gcccgtcccg gtactccgct cccagccgcc agacaatcaa ttttgcgtac
     2401 accggtgcgc tcccttacgc cggccaccgc cggtccgaac gtgccaaaga actgaacgtt
     2461 ggcgtggaaa tcgaccccac acaccgggac gaatacgatc gcaaccggca gctattccga
     2521 ctggctcacg attggattga gggcgagccg aacgccccag ccgcgtccac aggcacagcg
     2581 gacgtctgcc tcgtcgaatc ccgaaacggt cgcgtgcgac tcttccccgc acgagcacag
     2641 taccgtgacc gcggcgtatt gggcacgcaa ctccgggtcg cgcacgtcgg gaaacggg
//
LOCUS       DASSGX010000343         2413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_168361, whole genome shotgun sequence.
ACCESSION   DASSGX010000343 DASSGX010000000
VERSION     DASSGX010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168361"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="VFI12_06070"
     CDS             complement(<1..502)
                     /locus_tag="VFI12_06070"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HET7056008.1"
                     /translation="MTARRLVLGNWKMNTTLEEAVDIAERLRAEKSFDGVSVGIAPPF
                     PWLAAVKQALGDADVLLGAQTLAATANGAFTGEVSAAMVAELCDFVLIGHSERRTMLG
                     ETDEIVVEKLRRAVEAGLIPVVCVGETLAQRLAGDQERIVIEQISAALSGFPNEALHR
                     LVIAYEP"
     gene            complement(499..1680)
                     /locus_tag="VFI12_06075"
     CDS             complement(499..1680)
                     /locus_tag="VFI12_06075"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HET7056009.1"
                     /translation="MAIRSYEDADVSGKRVLVRVDFNVPLDGSEIRDDTRIRAALPTI
                     RNLLDRGAAVILISHLGRPKGQPNPKYSLAPVAKRLSTLLDRPVSFVPDIVGQQAVNT
                     ARQLQPGNVLLLENVRFEPGEEKNDPAMASKLAALGDVYVNDAFGAAHRAHASTEAIA
                     HLLPAYAGALMLGEIDALQQLVDSPREGFVAIIGGAKVSDKIAVLERLVDRVDSLLIG
                     GGMANTFLLERGREIGTSLAEADATDDARRVVDLAKQRDTTILLPADVVVAPGIDSDK
                     TSVVSIEAVPPDQGIFDIGPETIDAFANVIRNAKTIFWNGPMGVFEQPQFAIGTTAIA
                     NAVAESDAFSVIGGGDSVAAVEAAGVAGKISHISTGGGASLEFVEGRRLPGIEAFREE
                     S"
     gene            complement(1691..>2413)
                     /locus_tag="VFI12_06080"
     CDS             complement(1691..>2413)
                     /locus_tag="VFI12_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HET7056010.1"
                     /translation="TDAEKARGHIEAGAKKVIISAPGKGDDVTICMGVNEDQYDAEKD
                     VIISNASCTTNCLAPVAKVVLDNFGIKRGLMTTVHSYTNDQVILDGPHKDPRRARAAA
                     TNIIPTSTGAARALALVIPELKGKFDGFSLRVPTPTVSIIDFVAETEKGTTVEELNAA
                     FKAASESDAMRGILGYTEEPLVSSDFRGDPRSAIIDAESTMVIGDTMVKVIAWYDNEW
                     GYSCRIADLTALICEAGIPGTA"
BASE COUNT          406 a          787 c          766 g          454 t
ORIGIN      
        1 ccggctcata ggcgatcacc agcctgtgaa gcgcttcgtt cgggaagcca gagagcgcgg
       61 cgctgatttg ctcgatgacg atccgttcct gatcgccagc cagccgttgg gccagggtct
      121 cgccgacgca cactaccgga atcaggccgg cttcgacggc acgccgcagc ttctcgacta
      181 ctatctcgtc cgtctcgccc agcatcgttc tccgctcgga gtggccgatc aggacgaagt
      241 cgcaaagctc cgcgaccatg gccgccgaca cttcccccgt gaacgcgccg ttggcggtcg
      301 ccgccaacgt ctgcgccccg agcagcacgt cggcatcgcc aagagcttgc ttgactgcgg
      361 cgagccaggg gaatggcgga gcgatgccga cgctcacccc gtcgaacgat ttttcggccc
      421 gaagtcgctc ggcgatatcg accgcttcct caagcgtggt gttcatcttc cagttgccga
      481 gcacgagtcg tcgcgcggtc atgattcctc ccgaaacgcc tcaattccgg gcaacctgcg
      541 gccctcgacg aactccagtg atgcgccgcc acctgtcgag atgtgcgaga tcttaccggc
      601 cacgccggcc gcctcgaccg ccgcaaccga gtcgccgccg ccgatcaccg aaaatgcatc
      661 cgattcggcg accgcattcg caatcgcggt cgtgccaatc gcaaactgcg gctgctcgaa
      721 cacgcccatc ggtccgttcc agaagatcgt cttcgcattc cgaatcacgt tggcaaaggc
      781 gtcgattgtc tcaggtccga tgtcgaagat cccctgatcc ggtggcacgg cctcgatcga
      841 cacgacagag gtcttgtcgg agtcgatacc cggcgccacg actacgtcgg ccgggagcaa
      901 aatcgtcgtg tctcgctgtt tggcaaggtc gacgactctt cgcgcgtcgt cggtcgcgtc
      961 cgcctccgcc agtgatgtgc cgatctcgcg gccgcgctcc agcaaaaagg tgttggccat
     1021 accgccgccg atcaggagtg agtcaacccg atcgacgagc cgctcgagga cggcgatctt
     1081 gtcggagacc ttcgccccgc cgatgatcgc gacaaacccc tcgcgcgggc tgtcgaccag
     1141 ctgctggagg gcgtcgatct cgccgagcat aagtgcgccg gcatatgcag gcaggagatg
     1201 cgcgatcgct tcggtagatg cgtgggcccg gtgcgccgcg ccgaacgcgt cattgacgta
     1261 tacgtcccca agcgcggcga gtttcgaggc catcgcgggg tcgttcttct cctcgcccgg
     1321 ctcaaaccgc acgttctcga gcaggagaac attccccggc tggagttgac gggcggtatt
     1381 gacggcctgt tgccccacga tgtccggaac gaaggacacc ggacggtcga gcagcgtcga
     1441 caaccgtttg gcgaccggcg caagtgagta tttcgggttc ggctggccct tgggtcggcc
     1501 gaggtggctg atcaggatga ccgccgcgcc gcggtccagc aggttccgga tggtgggcag
     1561 cgcggcccgg atcctcgtgt cgtcgcgaat ctcgcttccg tcgagcggca cgttgaaatc
     1621 cacgcggacc aggacgcgct ttccgctgac atcggcatct tcataggagc ggattgccat
     1681 cgcggccagc tcaggcggtg ccggggatgc cggcctcgca gattagcgcg gtcaggtcgg
     1741 cgatacggca ggagtaaccc cactcgttgt cgtaccaggc gatgaccttg accatggtgt
     1801 cgccgataac catggtcgat tcggcgtcga tgatcgccga gcgcgggtca ccccggaagt
     1861 cggacgagac gagcggttcc tcggtgtagc caaggatgcc gcgcatcgcg tcgctttcgg
     1921 atgccgcctt gaaggccgcg ttcagctcct cgacggtcgt ccctttttcg gtctcggcca
     1981 cgaagtcgat gatcgagacg gtcggcgtcg gaacccgaag tgagaagccg tcgaacttgc
     2041 ccttcagctc gggaatgacg agggcgagcg cccgcgccgc tccggtcgaa gtcgggatga
     2101 tgttggtcgc agccgcgcgg gcccggcgcg gatccttgtg cggtccgtcg aggatgacct
     2161 ggtcgttggt gtaggaatga accgtggtca tcaatccgcg cttgatgccg aagttatcga
     2221 gcacgacctt ggcgaccggc gccaggcagt tcgtggtgca cgacgcgttc gagatgatga
     2281 cgtccttctc ggcgtcatac tgatcctcgt tgacgcccat gcaaatagtg acatcgtcgc
     2341 ctttgcccgg cgcggagatg atgaccttct tcgccccggc ttcgatgtgg ccgcgcgcct
     2401 tctcggcgtc tgt
//
LOCUS       DASSGX010000344         4078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1586300, whole genome shotgun sequence.
ACCESSION   DASSGX010000344 DASSGX010000000
VERSION     DASSGX010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1586300"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..1056
                     /locus_tag="VFI12_06085"
     CDS             118..1056
                     /locus_tag="VFI12_06085"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7056011.1"
                     /translation="MMATTDAAPLTSVTAPTEGIRRKSRTNFQLARQRFFRQRLAVVG
                     LVIVIAFLLCAIFAPLLAPTGYAAANLMDANKFPSREFPFGTDTIGHNMLSRVIFGIR
                     TSMLVAFSAVGLACLIGIPLGLAAGLKGGWLDFIVLRIVEIMTALPGILFAIFLMSVL
                     SSGAFDHILVGGQVTNVVVVIAITSWVPLCRLMRAQILQLREQEYVTAARSIGASEYR
                     VAVTHLLPNAIPPMIVAITLGIPAAIFAEAGLSFLGIGINEPVPSLGKMVSDSRQYIQ
                     LYWHLGLFPSLAIALAVLGFAFLGDGLRDALDPRQI"
     gene            1102..2904
                     /locus_tag="VFI12_06090"
     CDS             1102..2904
                     /locus_tag="VFI12_06090"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7056012.1"
                     /translation="MNEIDKLTRSDETQEEPRRVTRRRVISTTAAAAAAGIFGSGLLE
                     QAGAAPLNPARHITTLRQGTPPADAAPPEKQVLVFPDNVNTAKVIDFYQAVYERPSAS
                     FADILSEPLVRLNRNFEIIPGQAESWEGSEDGKTWTFKLKKELTWSDGNPLTAADWIA
                     TFQWGANPEHAWDFTWFFDGVLKNWTQAIAGDAGLDTIGVRQGADEYELVIETEVPAP
                     YIPAMMLYSLPLSKAALDATGSGLYNSDPATSVTCGPYMLTEWTQQQQLVYTKNPKYT
                     GSMKVMPEKLVVKLATPDAYFTMYQNNEIDYIEAPDPASLTLMMADETTAKEVHSGVG
                     DFPTWYIFFDVTKAPFDNKMVRQAWSHAIDRDTLKSQVLGPNGTQAYSWLAPGFPASQ
                     REALQDIQKFDPAMAKDLLSQAGFPDGKDFPKQTMWLRAPSPLDKTVAGALAAMIKQN
                     LNIDVELEEHDSQGFTAALNAKPTQILLGYVRYGMDYLDPSNMLSVWKSGGRHSWSNP
                     EYDAKLKEASEYLGDPAERTKLFQEAERILVEDVPGVFVYHGTPVQLIKPWLKGDFVA
                     PDKNGIAALHWPNFTTMDTVPEEIYVGSDAPDRG"
     gene            complement(3370..>4078)
                     /locus_tag="VFI12_06095"
     CDS             complement(3370..>4078)
                     /locus_tag="VFI12_06095"
                     /inference="COORDINATES: protein motif:HMM:NF017509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056013.1"
                     /translation="TEEEFREEIRPDVVKRLRNSLVLQEIAKRENIAVSDDEVESEIE
                     TIVSGAPNPDQMRQVYSGDRYMRSVLRNEMYDERLSNFLIDTATEGRGATLNGYVPGA
                     EETTPGAEAAEEKPKAKKSSKKKETAEEPGGAKADSPRGSVEGSGESACPEGYPIKGN
                     ASSMIYHLPGQSSYENTIPEICFATEEDAEAAGYRASKARGAAAAAGQAVADSAPGAP
                     EGEAETESLSGGEIVGE"
BASE COUNT          677 a         1382 c         1304 g          715 t
ORIGIN      
        1 ggcccttgtg ctgttggtcg cggccgtctg gggcttgacc tatctgatta ccgacgtcct
       61 ctacacgatc ctcgatccgc gcatccgaat tggcggcgga tcgatccacg gagcgtgatg
      121 atggcaacga ccgatgccgc tccactcaca tcagtgactg cgccgacgga aggtattcgg
      181 cgcaagagcc gcacgaactt ccagcttgcc cggcaacgct tcttccggca gcggctggcg
      241 gtggtcggcc tggtaatcgt gatcgccttc ctgctctgcg cgatcttcgc gccgctgctc
      301 gcgccgaccg gatatgccgc cgccaacctg atggacgcca acaagttccc gagccgggag
      361 ttccccttcg gcaccgatac gatcggccac aacatgctca gccgggtcat cttcggcatc
      421 cgcacctcga tgctggtcgc cttctccgcg gtcggcctgg cctgcctgat cgggatcccg
      481 ctcgggctgg ccgcggggtt gaagggcggc tggctcgatt tcatcgtgct gcggatcgtc
      541 gagatcatga cggcgctgcc cggcatcctg ttcgcgatct tcctgatgtc ggtcctgagc
      601 agcggcgcgt tcgatcacat cctggtcggc gggcaggtca cgaacgtggt ggtcgtgatc
      661 gcgatcacga gctgggtgcc gctctgccgg ttgatgcgcg cgcagatcct gcaactccgg
      721 gagcaggaat atgtgacggc ggcgcggagc atcggcgcga gcgagtaccg ggtcgcggtc
      781 acgcatctgc tgccgaacgc gatcccgccg atgatcgtgg cgatcacgct cggcattccg
      841 gcggcgatct ttgccgaggc cggcctcagc ttcctcggga tcggcatcaa cgagccggtg
      901 ccgagcctcg gcaagatggt atcggacagt cgccagtata ttcagctgta ctggcatctc
      961 gggctcttcc catcgctggc catcgccctg gccgtgctgg gattcgcatt tctgggggat
     1021 ggtctgcggg acgcactcga cccgcgccag atataacgag ggctgcccga tggcgggccg
     1081 gccacgagga aaggatcacc ggtgaacgaa atcgacaagc tcacccgttc cgatgagact
     1141 caggaggagc cacgccgggt cacgcgccgg cgcgtcatct cgacgaccgc cgccgcggcc
     1201 gctgccggca tcttcggcag cggcttgctc gagcaggccg gcgccgcgcc gctcaacccg
     1261 gcccgccaca tcacgaccct tcggcaaggc acgccgccgg ccgacgccgc gccgccggag
     1321 aagcaggtcc tggtcttccc ggacaacgtc aacaccgcca aggtgatcga cttctaccag
     1381 gcggtttacg agcgcccctc ggcctcgttc gccgacatcc tcagcgagcc gctggtccgg
     1441 ctgaaccgga acttcgagat cattcccggc caggccgagt cctgggaggg cagcgaagac
     1501 ggcaagacct ggaccttcaa gctcaagaag gagctgacct ggagcgacgg caacccgctg
     1561 accgcggccg actggatcgc gacctttcag tggggcgcca acccggagca tgcctgggac
     1621 ttcacgtggt tcttcgacgg cgtgctcaag aactggaccc aggcgatcgc cggcgatgcg
     1681 gggctcgaca cgatcggcgt ccgccagggc gcggacgagt atgagctggt gatcgagacc
     1741 gaggtcccgg cgccgtacat cccggcgatg atgctctact cgctgccgct ctccaaggcg
     1801 gcgctggatg cgaccggcag cgggctctac aactccgacc cggcgacgtc ggtcacctgc
     1861 ggcccgtaca tgctgaccga gtggacccag cagcagcagc tcgtctacac gaagaacccg
     1921 aagtacaccg gctcgatgaa ggtcatgccg gagaagctgg tcgtcaagct ggccacgccg
     1981 gacgcctact tcacgatgta ccagaacaac gagatcgatt acatcgaggc gccggatccg
     2041 gcctcgctga ccctgatgat ggccgacgag acgacggcca aggaggtcca ctccggcgtc
     2101 ggcgacttcc cgacctggta catcttcttc gacgtcacca aggcgccatt cgacaacaag
     2161 atggtccgcc aggcctggag tcacgcgatc gaccgcgaca cgctcaagtc ccaggtgctc
     2221 ggcccgaacg ggacgcaggc ctactcctgg ttggccccgg gcttcccggc gtcgcagcgc
     2281 gaggcgctcc aggatatcca gaaattcgac ccggccatgg ccaaggatct cctcagccag
     2341 gccggcttcc cggacggcaa ggacttcccg aagcagacga tgtggctgcg cgcgccgagc
     2401 ccgctcgaca agacggtcgc cggcgcgctg gcggcgatga tcaagcagaa cctgaacatc
     2461 gacgtcgagc tggaggagca cgactcgcag ggattcaccg ccgcgctgaa cgccaagccg
     2521 acccagatcc tgctcggcta cgtccgctac ggcatggact acctcgaccc gtcgaacatg
     2581 ctctcggtct ggaagtcggg cggccgccac tcgtggtcga acccggagta cgacgccaag
     2641 ctcaaggagg cgtccgagta cctgggtgac ccggccgagc gcacgaagct gttccaggag
     2701 gcggagcgga tcctggtcga ggacgtgccg ggcgtcttcg tctaccacgg cacgccggtc
     2761 cagttgatca agccgtggct gaagggcgac ttcgtggcgc cggacaagaa cggcatcgcc
     2821 gcgctgcact ggccgaactt cacgacgatg gacacggtgc cggaggagat ctacgtcggc
     2881 tccgacgccc cggaccgcgg ctgatcaagg tctctggccg ttggtctttc gtctctgata
     2941 caggggcggg ggcaggggag caatctcccc tcctccgcct ctttccttct agggatcggt
     3001 ctcacggtag gggtccgcgg gggcggaccc gcgtagttcc ccaagcccga tggtgtggag
     3061 cgacatcacg gttcggcgtg gtcccaggca cggacatatc ggccgactaa agcgggtttc
     3121 agaaaacgtg cctgctgccc aatgtagccc cgggcggctc gcccggattt ggtgtgacac
     3181 ggccgggcga gaccgcacgg ggctacgctt tctgaaccgg tacttcttcc gggcaccgct
     3241 ctagacgcaa acgtgccccc gcacgtccct acaacagggc agcggattcg ccgaacgatg
     3301 gctgtgacac aacgaggccg gatgcaatgt gcatccggcc tcgttccatg tttcggttgt
     3361 tcgcaagagt tactcgccga cgatttcgcc gccggagagg gactcggtct cggcttcgcc
     3421 ttcgggagct cccggggccg agtccgctac cgcctggccg gctgcagccg cggcgccacg
     3481 cgccttgctg gcccggtagc ccgcagcctc ggcatcctct tcagtcgcga agcagatctc
     3541 cgggatcgtg ttctcgtagg agctctggcc cggcaggtgg tagatcatcg acgaagcatt
     3601 gcccttgatc gggtaaccct ctggacaggc ggactcgccg ctgccctcga ccgaacccct
     3661 tggcgaatcc gccttggcgc cgcccggttc ctcggcggtc tccttcttct tgctgctctt
     3721 cttcgccttc ggcttctctt cggccgcctc agcccccggg gtggtctcct cggcgcccgg
     3781 gacgtagccg ttgagcgttg cgccccggcc ctcggtcgcg gtgtcgatca ggaagttcga
     3841 gagccgctcg tcgtacatct cgttgcggag aactgagcgc atataacgat cgccgctgta
     3901 gacctgccgc atctggtccg ggttcggcgc accggagacg atggtctcga tctccgattc
     3961 gacctcatcg tccgagaccg cgatgttttc ccgcttggcg atctcctgca gcacgagcga
     4021 gttgcgcagc cgcttgacga cgtcgggacg gatctcctcg cggaactcct cctcagtc
//
LOCUS       DASSGX010000345         3174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_933245, whole genome shotgun sequence.
ACCESSION   DASSGX010000345 DASSGX010000000
VERSION     DASSGX010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933245"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VFI12_06100"
     CDS             complement(<1..240)
                     /locus_tag="VFI12_06100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HET7056014.1"
                     /translation="MEVEDRPLIVASNRGPVTFTREADGSFTSRKGSGGVVTAVTGIA
                     KGRQPVWVACPMTLGDRRRAELARERDEEFIIPEGL"
     gene            539..862
                     /locus_tag="VFI12_06105"
     CDS             539..862
                     /locus_tag="VFI12_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056015.1"
                     /translation="MAASSSPQTRDCPWCGSRKTKLVQRGYTGPTDEINQYYTCQGCG
                     KVTYELVSKTAREMRLGRYLPGGIYKDTANQTRYQITRVLKVGINEFLIYLKPMPGAD
                     DFDGR"
     gene            892..1209
                     /locus_tag="VFI12_06110"
     CDS             892..1209
                     /locus_tag="VFI12_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056016.1"
                     /translation="MAQLDPAPNYDHRLVEWVTLYLQTRDERIFPFTQYFDDERRAAF
                     IHDLRVALSELQDSGSARKTSATGFIVNDSTLHGIVRAWAMAGGGWPDTSNPANPIGL
                     AVD"
     gene            complement(1280..2419)
                     /gene="solA"
                     /locus_tag="VFI12_06115"
     CDS             complement(1280..2419)
                     /gene="solA"
                     /locus_tag="VFI12_06115"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF008425.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HET7056017.1"
                     /translation="MAARTWDVIILGGGTMGTAATWALSTRGVSVLVLEQFGHIHTMG
                     SHGGGTRIFRHAYAEGPEYVPLMFHADELWCSLENELGTHIVHRVGILEMDAPGYDHA
                     KRARSSAEKNGVELEWLDAAEIRKRWPAFAPPDDWEGGYSPIAGFLDIEAGLSGLAAV
                     AKRGDVTFRTDEPVVGWSASEGGVRVETAQGQYLANKLIVTAGAWASSMLAELELPLE
                     VVRKQNFWVDVDRPSSFQPEVFPVFATASDVGEIYGFPIFRRPGIKMAEHRGGDTTTP
                     QTIDRVARDDEAQSCVDLAALAMQGVSRRVLGGVICMYTRTPDDHFIVDRHPSAPNVV
                     LAAGFSGHGFKFTPAIGEQLANLTLDEDAEPFALFGIDRFAAVRS"
     gene            complement(2419..>3174)
                     /locus_tag="VFI12_06120"
     CDS             complement(2419..>3174)
                     /locus_tag="VFI12_06120"
                     /inference="COORDINATES: protein motif:HMM:NF018038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HET7056018.1"
                     /translation="SSRMSIQVTVWNEYRHELSDEDVRAVYPDGIHTAIAAGLQEHAD
                     LVVRSATLDEPEQGLPPEVLDSTDVLIWWGHKAHPEVADELVDRVQQRVLDGMGLIVL
                     HSGHHSKIFKRLMGTTANLKWREATDKERIWVVQPGHPIVHGVGEYIELPVEEMYGEF
                     FDIPQPDELITISWFTGGEVFRSGCCFFRGRGKIFYFRPGHETYPTYFDPAIRKVIAN
                     GVRWAAPVDGPIPDFGLNGPNAPLEPLPPREEQ"
BASE COUNT          611 a          985 c          995 g          583 t
ORIGIN      
        1 gagcccttcc gggatgatga attcctcatc ccgttcgcgc gccagctcgg cgcgtcgccg
       61 gtcgccgagg gtcatggggc acgccaccca gacgggctgc cgtcctttgg cgatacccgt
      121 caccgcggtg acgacaccgc ccgacccttt cctggaggta aacgaaccat ccgcctcacg
      181 ggtgaaggta accggcccgc gattcgaggc gacgatgagt gggcgatctt caacttccat
      241 ttgctacgcc ctcgccttcc tcgatgtcat cgtttcccgg gccgtgcgcg gattcgccgc
      301 ctggcgccct cgctgtcaac gagaggagcc gtcgcgttcc tcgcaccggt gcacgccgcc
      361 gtgcacatgc tcatagtatg cccggttctc tgttccagag gcagtagggg cgccagccgg
      421 cgcccaggca ttgccggcta tgtcaacgcg gatgtgtggt ctgaagcggt ctggccggcg
      481 ctggtacaat ccgcagacga tggaaggcac gggaccagat caggagcgac tctttttcat
      541 ggcggcgtcg agcagtccgc agactcgcga ctgtccgtgg tgcgggagcc gcaagaccaa
      601 actggtgcag cgggggtata ccggacccac cgatgagatc aaccagtact acacctgtca
      661 ggggtgtggg aaggtaacct atgagctggt gtccaaaacc gcgcgggaga tgcggctggg
      721 tcgctatttg ccgggaggaa tttacaagga caccgccaac caaacccgct atcaaatcac
      781 ccgcgtgctg aaggtcggca tcaacgagtt tctgatctat ctcaaaccga tgcccggtgc
      841 ggacgatttc gacgggcgct agcatatcgt cgtacaaggg aaaggcgtta cgtggcgcag
      901 ctcgatccgg ctccgaacta cgatcatcga ctcgtggaat gggtcacgct ctatttgcag
      961 acccgcgatg agcgcatctt cccattcacg cagtatttcg acgacgaacg acgggcagcc
     1021 ttcatacatg acctgcgcgt tgcgctctcg gagctgcagg atagcggcag cgcgcgcaaa
     1081 acctcggcaa ccgggttcat cgtgaacgac tcgaccctac acggcatcgt gcgggcctgg
     1141 gcgatggccg gcggtggctg gccggataca tcgaatcccg ccaatccgat cggattggcg
     1201 gtcgattgaa ggaggttgga ttttggggtc aggaattggc gccggtgggc caaacgtcct
     1261 acatcccaac tcctaaccgc taactcctca cggcagcgaa acggtcgatg ccaaagaggg
     1321 cgaagggctc cgcatcctcg tcgagcgtca gattcgccag ctgctcgccg atggcggggg
     1381 tgaacttgaa tccgtggccg ctgaacccgg cggcaagcac gacattcggc gcagatgggt
     1441 gccggtccac gatgaagtga tcgtcgggtg tgcgcgtata catgcaaatg acgccaccga
     1501 gaacgcgacg ggagacgccc tgcattgcca acgcggccaa atcgacacaa ctctgtgctt
     1561 catcgtcgcg cgcgactcgg tcgatggtct gcggggtcgt tgtgtcgccg ccgcgatgct
     1621 ccgccatctt gattccgggg cggcgaaaga tcgggaagcc gtagatctca ccgacatccg
     1681 aggccgtcgc gaagacagga aagacttcgg gttggaacga cgacggccgg tcgacgtcga
     1741 cccaaaagtt ttgcttgcga acgacctcca ggggaagctc gagctcggcc agcatgctgg
     1801 atgcccacgc gccggcggtc acgatcagct tgttggcgag gtactgaccc tgagccgtct
     1861 cgaccctgac gccaccttcg ctggctgacc agccgaccac cggctcgtcg gtgcggaatg
     1921 tcacatcgcc tcgtttcgcc acagcggcaa ggccggagag tccggcctcg atatccagga
     1981 acccggcgat cgggctgtat ccgccttccc agtcgtccgg gggagcgaac gcgggccagc
     2041 gcttgcgaat ctcggccgcg tccagccact ccaattcgac accgttcttc tcggccgagg
     2101 agcgggcgcg tttggcatga tcgtagccag gcgcatccat ttccagaatg ccgacccggt
     2161 ggacgatgtg ggtgccgagc tcgttttcga gcgagcacca aagctcgtcg gcatggaaca
     2221 tgagcggaac atattctgga ccttcggcat acgcgtgccg gaagatgcgc gtgccaccac
     2281 cgtgcgagcc catggtgtgg atatgcccga attgctcgag caccagtacg ctgactccac
     2341 gggtagaaag cgcccaggtg gcggccgtgc ccatggtgcc gccgccgaga atgatgacgt
     2401 cccaggtgcg agcagccatt actgctcctc gcgtggcggc agcggctcga gcggcgcgtt
     2461 tggcccgttc aacccgaagt cgggaatggg gccgtcgacg ggagcagccc agcgaacgcc
     2521 gttcgcgatg accttacgaa ttgctggatc gaaataagtc ggataggtct catggccagg
     2581 gcggaagtag aagatcttgc ccctgccgcg gaagaagcag caacccgagc ggaagacttc
     2641 tccgccggta aaccagctga tcgtgatcag ctcgtccggc tgaggaatat cgaagaactc
     2701 gccgtacatc tcttcgacag gaagctcgat gtactcgccg acgccatgca cgatcggatg
     2761 gcccggctgc acgacccaga tgcgctcctt atccgtagct tcgcgccatt tgagatttgc
     2821 ggtggtgccc atcagccgct tgaaaatctt ggagtgatgg ccggagtgga ggacgatcaa
     2881 ccccatcccg tcgagcacgc gctgctgaac tcggtccacc agctcgtcgg cgacctcggg
     2941 atgcgccttg tgtccccacc agatcagcac atcggtggaa tccagcacct ccggtggaag
     3001 cccctgttcg ggctcatcga gtgtggcgga gcgcacgacg agatcggcat gttcctgcaa
     3061 gccggcggct atggcggtat gaatcccgtc cggatagacc gcgcggacat cctcgtcgct
     3121 gagctcatgt cgatattcgt tccagacggt gacctggatg ctcattctgc tgct
//
LOCUS       DASSGX010000346         2513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_933877, whole genome shotgun sequence.
ACCESSION   DASSGX010000346 DASSGX010000000
VERSION     DASSGX010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933877"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..925
                     /locus_tag="VFI12_06125"
     CDS             122..925
                     /locus_tag="VFI12_06125"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7056019.1"
                     /translation="MTRYVFDNASEQPTAQRFANLERLYDGRTIAALEKTGVAAGWRC
                     LEVGAGSGSIATWLADRVGPSGQILATDIDPRFLETLASENRPNVEVRRHDIATDPLP
                     ERAFDLIHARLVLLHVPAAPAVLARLVTALRPGGWLVVEDFDPTFIDRSFPVTDPAQA
                     AVIHAAYAALGTLIDRSGAGRGWARGLSGHFMAAGLDDVAAEGHLSIRSGGSPGAQLD
                     AVNFAQVRNALIDGELLTTEVLDATLQLLGDAGCMVSSPMMFTAWGRRP"
     gene            complement(1261..1512)
                     /locus_tag="VFI12_06130"
     CDS             complement(1261..1512)
                     /locus_tag="VFI12_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056020.1"
                     /translation="MVMTVTRPQGVSAVPGQEITRPTAAGSVEITFQSGTTAVDQAPC
                     QIIIVQPEAGSARSSEYTVASSGFGFSDEEPTWEDAAWQ"
     gene            complement(1541..1678)
                     /locus_tag="VFI12_06135"
     CDS             complement(1541..1678)
                     /locus_tag="VFI12_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056021.1"
                     /translation="MLPYTAVDLIRSMGATIASPERDAPRSALRARIAGLVVRYRSTA
                     R"
     gene            complement(1891..>2513)
                     /locus_tag="VFI12_06140"
     CDS             complement(1891..>2513)
                     /locus_tag="VFI12_06140"
                     /inference="COORDINATES: protein motif:HMM:NF042779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TOPRIM nucleotidyl transferase/hydrolase
                     domain-containing protein"
                     /protein_id="HET7056022.1"
                     /translation="EELRTLNPVDRQAVILVEGISDQVAVETLAARLGHDLAGEGVSV
                     EPVGGAHAFGRALARLGSHEPSIRLAGLYDIGEERVVQRALERSGLSAGVPVTQAGIQ
                     RLGFYACVVDLEDELIRALGAAAVEAIIDAQGELTSFRTLQKQPSWRGQRIEAQLRRF
                     MGSGATRKIRYARLLVDALDLDQLPRPLRLVLAHVSSDSSPNALSR"
BASE COUNT          439 a          796 c          810 g          468 t
ORIGIN      
        1 tatattcagc ggacatggaa gccatttccc ctgtccttac gttcatgcca tactgctgtt
       61 gccgtcccac ggttcaactg cgcgaaatct gtcgttgatg cactgccgga ggggagtacc
      121 cgtgacccgc tacgtcttcg acaatgcgag cgagcaaccc accgcgcagc gatttgccaa
      181 cctggagcgg ctttacgacg gccggacaat cgccgccctg gagaaaacgg gcgtcgcggc
      241 cggatggcgc tgccttgagg tcggtgccgg aagtggttcc atcgccacgt ggctcgccga
      301 tagggtaggt ccctccgggc agattctggc caccgacatc gatcctcggt tcctggagac
      361 tcttgccagt gagaaccgcc cgaacgtgga agtacgtcgg catgacatag cgacggaccc
      421 attaccggag cgggcgttcg accttatcca tgctcgtctc gtacttcttc acgtcccggc
      481 tgcgccggca gtccttgccc gcctggtgac tgccctgcgc ccgggcggct ggctcgtcgt
      541 cgaggatttc gacccaacat tcatcgaccg gtcatttccg gtgaccgacc ccgcgcaggc
      601 tgcggtgatc catgctgcgt acgccgcgct cgggacactc atcgaccgca gcggtgcggg
      661 gcgcggctgg gcgcgcggat taagtgggca cttcatggcg gcgggattgg atgatgtcgc
      721 agccgaaggg cacctctcga tccggtccgg cggctcgcca ggagcgcaat tggacgccgt
      781 caacttcgcc caggtccgga atgcgttgat tgacggtgag ctgctcacca ccgaggtcct
      841 ggacgcaacc cttcaactcc tgggtgatgc ggggtgcatg gtttcctctc caatgatgtt
      901 caccgcctgg ggtcgccgcc cgtgaactga gcacgcacag tcggaatgac ggacaggatg
      961 gctcgtcggc aacgattggc gatgaccgac ccgggcgcct caggtcagag gcggatttcc
     1021 gtcggtttca ccgttcgcca ggagatgcgt tatcgccacg cagcgcagcg ggttctgcac
     1081 gctatgaagt cggcgcgctc catgatttcg ggtcctgcac ggcctgaaac acgtggcgga
     1141 tcgccgtgac tgccaggacg gccaatatcc gactcgccac cgaaccggga agcgccgagg
     1201 ggcccgaagg cccctctcgc agttggattg gattcatgac cccggggaac caggttgtcg
     1261 ttactgccag gcggcgtctt cccaggtcgg ttcctcgtcg gagaaaccga agccggacga
     1321 tgccacagtg tattcggatg accgagcgct accggcctcc ggctgcacga tgatgatttg
     1381 gcagggtgct tgatcaacgg ctgtggtgcc ggactggaac gtgatctcga cggagccggc
     1441 tgcggtcggt cgtgtgatct cctggccggg cacggctgat acaccctgcg gtcgcgttac
     1501 ggtcatgacc atcgtctcgt ctccttatgt ttgagccgcg ctagcgcgcc gtgctcctgt
     1561 agcgaacgac gagcccggcg attcgggcgc ggagggcgct gcgtggcgcg tcccgctcgg
     1621 gggacgcgat ggttgcgccc atggagcgga taagatcgac tgcggtatac ggcaacatcg
     1681 gacgctcctt tcggtagaaa ttaatcggtg acgcgaactg aaactctgcc ttggagggta
     1741 cgcgctcacc ggtccaggca catcggccaa gcggcggaga cgaacccaca tctcgtgacg
     1801 gaagccgttt ggcggaatga accaacgtca cggcaaagag aacggctgta cgcatctcct
     1861 tccgtggccg cccggggcta tatgttcaat tcatctggac agcgcatttg gagacgagtc
     1921 cgaagagacg tgagccagca cgaggcggag tggacgaggc aactggtcga gatcgagggc
     1981 gtcgacaagt aaccgtgcat accggatctt gcgcgtggcg ccgcttccca taaatctgcg
     2041 caactgcgct tcgatacgct gtcctcgcca tgagggctgc ttctgaagcg tgcggaagga
     2101 ggtcagctcg ccctgggcgt cgatgatcgc ctcgacggcg gccgcgccga gtgcccggat
     2161 caactcgtcc tcaaggtcga cgacgcatgc gtagaacccc agccgctgaa tgcccgcttg
     2221 agtcaccggg accccggcgc tgagcccgga tcgctcgagc gctcgctgga cgacacgctc
     2281 ctcccctatg tcgtagagcc cggccagcct gatgctcggc tcatgtgaac cgagtcgcgc
     2341 gagggcacgg ccgaatgcgt gcgccccgcc tacgggctcg acggaaacgc cctcgcccgc
     2401 aagatcgtgg ccgaggcgcg ccgcgagtgt ttcgacggcg acctggtcgc tgatcccttc
     2461 gaccaagatg accgcttgcc ggtcgaccgg attcaacgtc ctgagctcct ccg
//
LOCUS       DASSGX010000347         2738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1150909, whole genome shotgun sequence.
ACCESSION   DASSGX010000347 DASSGX010000000
VERSION     DASSGX010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150909"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /gene="truA"
                     /locus_tag="VFI12_06145"
     CDS             complement(<1..858)
                     /gene="truA"
                     /locus_tag="VFI12_06145"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HET7056023.1"
                     /translation="MARLKVKLVIAYDGTEFSGSQRQPGRRTIQDELERAVGILAHEP
                     VPVALAGRTDAGVHALGQVASFEDVRPDLEGRRLVSALNALLPEDVSVQSATRVEAEF
                     DPRRDARWRAYQYRICNGPENPLERRYTLQVTQRLDLVAMIDAASHFLGEHDLASFCG
                     LGAGVPWSERKNIGRGTVRRIFKCAVAQAEREPVVTTDVVGDAFLPNQVRAMVGALLD
                     VGRGRRDHDWIARLLTEPDRRHGPKTAPPHGLTLMQVGYSDVEGTAAEDRAEAETIQG
                     LVNDGSTDLF"
     gene            complement(884..1237)
                     /gene="rplQ"
                     /locus_tag="VFI12_06150"
     CDS             complement(884..1237)
                     /gene="rplQ"
                     /locus_tag="VFI12_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012660847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HET7056024.1"
                     /translation="MRHRKAGRKFGRNPAQRRALFRQLAVSMILHERITTTEAKAKSM
                     RSIVEKLVTIARDDSPAHRKLIMSKIDHPLATAKLFEVIAPRFTETPGGYTRIAKLEP
                     RRGDAAPMALIEFIA"
     gene            complement(1240..2337)
                     /locus_tag="VFI12_06155"
     CDS             complement(1240..2337)
                     /locus_tag="VFI12_06155"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HET7056025.1"
                     /translation="MFEKPQFRIDTEETGLNYGRYRIEPLEPGYGTTLGNALRRILLR
                     SLEGVAISRVQIEGVWHEFSTINNVREDVVEIVLNLKRVRLKRVTEFSGETRAHLYVR
                     ADSSGERVVTAGDIAWPSEVEIVNPDQVIATLTGTDAILDMDLWIARDRGYRPAEHQE
                     AYSIGQIPIDAIFTPVQKVNFVVEHTRVGQVTDYDRLILEILTDGTIEPEEALSEAAR
                     VLMDHARIFADFNRKEPEAAVTPGTFISEEVQNKALADLGLSPRVLNALRSRQIEKVG
                     QVLTMDPDQLLSIRNFGPRSLKELRDKLSEYGYLPEGDAGIFSADHGYFDALESAMDE
                     GEDDESRDVAEAIAALGGDDLGMNETNGEEA"
     gene            complement(2377..>2738)
                     /gene="rpsD"
                     /locus_tag="VFI12_06160"
     CDS             complement(2377..>2738)
                     /gene="rpsD"
                     /locus_tag="VFI12_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HET7056026.1"
                     /translation="NLLQLLESRLDNVVYRMGFADSRAQARQLVRHGHFVVNGRKTDI
                     PSFIVKPSDVVTVKAEKRSKTYFEDFNVQLETKRPPDWVSVNVNDLSGKVLSLPTRDQ
                     MELPPFNEALIVEYYSR"
BASE COUNT          480 a          899 c          815 g          544 t
ORIGIN      
        1 gaaaaggtcc gtcgatccat cgttcaccag tccctgtatc gtctctgcct ccgcgcgatc
       61 ctcggcggcg gttccttcaa cgtccgaata accaacctgc atcagcgtca agccatgtgg
      121 cggcgctgtc ttcggtccgt gtctgcgatc aggctcggta aggagcctcg cgatccaatc
      181 gtgatcacgc cgaccgcgac cgacatccag gagcgctccg accatggcgc gcacctggtt
      241 cgggagaaat gcatctccca cgacatcagt cgtgacgacc ggctctcgct ccgcttgcgc
      301 aaccgcgcat ttgaagattc gccggacggt tccacgtccg atgttcttcc gctccgacca
      361 cgggacacct gccccaagtc cgcagaagct ggcgagatca tgctcgccca gaaaatggct
      421 cgccgcgtcg atcatcgcca cgagatcgag tcgttgcgtc acctgcaacg tgtaccggcg
      481 ctccagcggg ttctcaggcc cattgcaaat ccggtactgg tacgctcgcc accgcgcgtc
      541 ccggcgcggg tcaaactccg cctcgactcg cgttgcggac tgaaccgata cgtcctccgg
      601 caagagcgca ttcaacgcgc ttaccaggcg gcggccctca agatcgggcc ggacatcctc
      661 gaacgatgca acctggccta gtgcgtgcac acctgcatcg gttcggcccg cgagcgcgac
      721 cgggaccggc tcatgtgcca gaatcccgac tgctcgctcg agctcgtcct ggattgtccg
      781 cctgcctggc tgccgctgcg agccagagaa ctccgttcca tcgtacgcga tgaccagctt
      841 gaccttaagc cgtgccaatg ctcgcgacga atcgtcagtc aactcaggcg atgaattcga
      901 tcaacgccat cggggccgca tcaccccggc gcggctcaag cttcgcgatt cgcgtgtacc
      961 cacccggtgt ctcggtgaac cggggcgcaa tcacttcgaa cagcttcgcg gtcgcaagcg
     1021 ggtggtcgat cttcgacatg atcagcttgc gatgcgccgg gctgtcgtcg cgcgcgatcg
     1081 taacgagctt ctccacgatc gagcgcatgc tcttggcctt ggcctcggtc gtcgtaatcc
     1141 gctcgtggag gatcatcgac acggccaact gccggaacag cgcccgtcgc tgggctgggt
     1201 ttcggccgaa tttgcggccc gccttacggt gtcgcatcgc tatgcttcct cgccatttgt
     1261 ctcgttcatg ccaagatcat cgccaccgag agctgcgatg gcctcggcca cgtcgcggct
     1321 ctcgtcgtcc tctccctcat ccatggccga ttcgagcgcg tcgaagtagc cgtggtcggc
     1381 ggagaagatg ccggcatcgc cttccggcaa gtagccgtat tcgctgagtt tgtcccgcag
     1441 ctccttgagg gaacgcggtc caaagttgcg gatcgacagg agctgatcgg gatccatcgt
     1501 caggacctgg ccgacctttt cgatctgccg gctacgcagc gcgttgagga cgcgcggcga
     1561 gagaccgagg tcggccaacg ccttgttctg gacttcctcg gagatgaagg tgccaggcgt
     1621 aaccgccgcc tccggctcct tgcgattgaa gtccgcgaag atgcgcgcgt ggtccataag
     1681 gacccgcgcc gcctcgctca gcgcctcttc cggctcgatc gtgccgtcgg tcaggatctc
     1741 gaggatcagc cggtcgtagt ccgtgacctg gccaacgcgg gtgtgctcca ccacgaagtt
     1801 gaccttctgg accggagtga agatcgcgtc gattggaatc tggccgatcg agtaggcctc
     1861 ctgatgctcg gccgggcgat atccgcggtc ccgggcaatc cacaggtcca tatcgaggat
     1921 cgcatcggtg ccggtcagcg tcgcgatcac ctggtcgggg ttaacgatct cgacctcgga
     1981 cggccaggcg atgtcaccgg ccgtgaccac gcgctcgccc gagctatccg cccggacata
     2041 gaggtgcgcg cgggtctcac cgctgaattc ggtaacccgc ttgagtcgca cccgcttcag
     2101 gttcaggacg atctcgacga catcctcgcg gacgttattg atcgtcgaga actcgtgcca
     2161 gacgccctca atctgaaccc gggaaattgc cacgccctcg agggagcgga gcaggattcg
     2221 ccggagcgca ttgccaaggg tcgtgccata accgggctcg agtggctcga tccggtaacg
     2281 accgtaattc aggccggtct cttctgtgtc gattcggaat tgcggtttct cgaacaagcg
     2341 agatcgcctc cttcttaatg cgctatgcgc cggagattac cggctgtagt actcaacgat
     2401 cagcgcctcg ttgaacggcg gcagctccat ctggtcgcgg gtcgggaggc ttagcacctt
     2461 gccgctcaga tcgttgacgt tcacgctgac ccaatcgggc gggcgcttcg tctcgagctg
     2521 cacattaaag tcttcaaaat acgtcttcga ccgcttctcg gccttgacgg tcacgacatc
     2581 gctcggcttc acaatgaacg agggaatgtc ggtcttccgg ccgttgacca caaaatggcc
     2641 gtgccgtacg agctgacgcg cctgggcgcg gctgtcggcg aagcccatgc ggtacacgac
     2701 gttatcaagg cggctctcga ggagctggag aaggttct
//
LOCUS       DASSGX010000348         4407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_169345, whole genome shotgun sequence.
ACCESSION   DASSGX010000348 DASSGX010000000
VERSION     DASSGX010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4407
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169345"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(333..980)
                     /locus_tag="VFI12_06165"
     CDS             complement(333..980)
                     /locus_tag="VFI12_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HET7056027.1"
                     /translation="MRHLRLLVTGLFVALILVVSQAQMSSAQTAGASLEIHKRVCPAD
                     ATGNIFDDCHGNPPTQTTTFSVDGGAATAVDASGNVTFTGLAAGAHTVTEVEGIPLEF
                     ANLRVFCSIEGDGTGAVEVATTGPDFSVTLADGQHMICDSYSLPINLSGLTPTPGTGG
                     TTPTPAIQLPNTGSGFDGGSGPLGLMSIFMVAIGLGVAALALVRRPVSKRVRAER"
     gene            complement(1135..1821)
                     /locus_tag="VFI12_06170"
     CDS             complement(1135..1821)
                     /locus_tag="VFI12_06170"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HET7056028.1"
                     /translation="MEDDSRAQSSAERTVRIPVGSVVLEGSLVVPADAVGVVLFAHGS
                     GSGRHSPRNRAVANVLQAGRIGTLLIDLLTEKEEEVDQTTRHLRFDIPLLAERLTGAI
                     DWLGAEPMTASLPIGLFGASTGGGAALLASVARPEPVRAVVSRGGRPDLADDALPEVI
                     APTLLIVGGLDDVVIALNQVAMEQMRCEKELRIVPGATHLFEEPGTLEQVADLARDWF
                     SVHLSRTARL"
     gene            complement(1965..3137)
                     /locus_tag="VFI12_06175"
     CDS             complement(1965..3137)
                     /locus_tag="VFI12_06175"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HET7056029.1"
                     /translation="MTRLAVILGIGLIGVILIDAFETMILPRRVTRRFRLTRLIVLNG
                     WRIWARAARLLPSSGNREGGGLREGFLGGFGPLALLLLLGTWAAGLVTGFGLLIWGTG
                     TPLGGPDPHPGLDAVLYLSGETFFTIGYGDLAPKAGLGRFLAVTEGATGFSFLAVVIA
                     YLPVIFTAYSQRETRITLLDARAGSPPTAAAFLARQASGSQPFDLDEYFREWELWSAE
                     LLEIQLSFPILVSFRSQHDRQSWLGALTVILDTTALVIAGLETSEGPVPGRQARLTFA
                     VARHAVGDFCQILVAPPSPPASDRLPAADLGRLREQLAASGLVLRLDADRLTALRQLY
                     EPYVAALAARIQVAVPPWLPAADAQDDWETTAWQWDPEVARTAIREPRDTGTVAGH"
     gene            complement(3134..>4407)
                     /locus_tag="VFI12_06180"
     CDS             complement(3134..>4407)
                     /locus_tag="VFI12_06180"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HET7056030.1"
                     /translation="RLMGGTSAVNGSVWLRGAPADYDAWAAMGNPGWGYADLAPWFRK
                     AENDPLEGTTGPVPIRRLDDGQPNEVERALIDAAGASGIPWVDNQNLPDRQDVCISGA
                     PRNVVENVRMNGALTYLAAARNRPNLEIIPGAPVDCVAITDGRATAVQTVDGRIFAGR
                     ETVLAAGAYGSPAILMRSGIGPADHLREFGIPVARDLPGVGANLLDHPLAANGFCLHL
                     VHPNAVPDRQAFIRLVIKGRSRQLADGIDYFLFSMVNNDPDAGGWVFVLPTSLMVARS
                     QGTVRLTSPDPNATLAIDHNHFADPADLEAICDGIELARRLAATPPLAEILSPARASL
                     AWSSREELRELVPAHADTTYHPSSTCRMGPADDPGAVVDLAGRVHGVRGLRVVDASVF
                     PTSPRANIHATVVAVAEKLAYALRGESAGPA"
BASE COUNT          766 a         1543 c         1427 g          671 t
ORIGIN      
        1 gacgcagtcg cggtgctcag ctgccgcgcg ggctggtcgt ccagcacccg cttgagggcg
       61 acctcccgaa atgtgccgtt gaggatcgct accgggatca tggcgaccca ggtcaatgcg
      121 gctctgcgaa tcatcacgtc actcctctgg gggaacgcga aaccatgcgt ccacctccag
      181 atccgagcat aggctcgctc ccgcgaggat cgcgtgtcgc ggggaatccc ggtatcacaa
      241 tttcgtgtca atgaattgct gaataccggg cacagaaaac gacagcggca caccgtgtgc
      301 cgctgtcgtc tcgagcgtac tttcggcgcg cgttaccgtt ccgcgcggac tcgcttggaa
      361 actggccggc gaaccagcgc cagcgcggcg acgccgagtc cgatcgccac catgaagatc
      421 gacatcaggc cgagcgggcc cgatccaccg tcaaagccgc tgccggtgtt cggcagctgg
      481 atcgccggcg tcggcgtggt gccgcccgtg cccggggtcg gcgtcaggcc ggacagattg
      541 atcggcaggc tgtacgaatc gcagatcatg tgctggccgt cagccagggt gacgctgaag
      601 tccggtcctg tggtcgcgac ttcgacagcg ccggtcccat cgccctcaat cgagcagaaa
      661 acccgcaagt tggcgaactc gagcgggata ccctcgactt cggtgaccgt gtgcgcaccg
      721 gccgccagcc cggtgaaggt cacgttgccg ctggcgtcaa cggccgttgc cgcgccgcca
      781 tcgaccgaga aggtagtggt ttgcgtgggc gggttgccat gacagtcgtc gaagatgttg
      841 ccggtcgcat ccgccgggca gacccgcttg tggatctcca ggctagcgcc ggctgtctgc
      901 gccgagctca tctgggcctg gctgaccacc aggatcaacg cgacgaacag gccggttacc
      961 aacagtcgca ggtgcctcat cgttttcctc ctcctcggac ggccgcgcgg ccgcctgacg
     1021 aacaccgaaa aaacgaaccg aggtgctcat gcccatgatg cactgtcgtt aagacgcact
     1081 ttacatggca gaggtttcgg ccgctacaaa gcatcccgaa ccttgccctg gacgttacag
     1141 gcgcgcggtc cgcgaaaggt ggacgctgaa ccagtcgcgc gccaggtcag cgacctgctc
     1201 gagcgtcccc ggttcctcaa aaaggtgcgt cgcgcccggg acgatccgca actccttttc
     1261 gcagcgcatc tgctccattg ctacctggtt gagcgcgatc acgacatcat cgagcccacc
     1321 gacgatcagc agcgtcggcg cgatcacctc cggcagggcg tcgtcggcca ggtccggccg
     1381 gcccccgcgc gaaacgactg cccgaaccgg ttccggccgc gcaaccgacg ccagcaacgc
     1441 cgccccgccg ccggtgctcg ccccgaacag gccgatcggc agggacgcag tcatcggctc
     1501 tgccccgagc cagtcgatcg cgccggtcag ccgctccgcc agcagcggga tgtcgaaccg
     1561 caggtgccgc gtcgtctgat cgacctcttc ctccttctcg gtcagcagat cgatgagcag
     1621 cgtcccgatc ctgcccgcct ggagcacgtt tgcgacggcg cggttgcgcg ggctgtgccg
     1681 tccgctgccg ctcccatgcg cgaacagcac cacgccgacg gcgtccgccg gcaccaccag
     1741 gctgccttcg agcacgacag aaccgacggg aatcctcacg gttcgctctg cgctggattg
     1801 cgcccggctg tcatcctcca ttgccccgcg tcacctcctg gttcgtcgcc gtaccgacgc
     1861 taaccgtgcc gccgcgaatt catcgtgagg agcctcgttc catcctggtg cgcgtacaaa
     1921 caacgagatt tccctcttgg atgtagcccc ggtcagccaa cacattaatg accggccact
     1981 gtgccggtgt cgcgaggttc ccggattgcg gtacgcgcca cctccggatc ccactgccag
     2041 gcagtggtct cccaatcatc ctgagcatct gccgccggca gccacggcgg caccgcgacc
     2101 tggatccgcg cagcgagagc agcaacgtac ggctcgtaca gttgccgaag ggcagtcagc
     2161 cggtcggcat ccagccggag cacgagcccc gaagcggcca attgctcccg caaccgcccg
     2221 agatctgcgg cgggcaaccg gtcactcgcc ggcggcgaag gcggggcgac caggatttgg
     2281 cagaaatctc caaccgcgtg ccgggccacc gcgaacgtta atcgagcctg ccgcccgggg
     2341 accgggccct cgctcgtctc gagtccggcg atcacgagcg cggtcgtatc gaggatcacc
     2401 gtcagggcgc cgagccacga ctggcggtcg tgctgcgagc gaaaagagac caggatcggg
     2461 aaggagagct gaatttcgag taactcggca gaccacagct cccactcccg gaagtattcg
     2521 tcgaggtcga acggctgcga tccactcgcc tgtcgagcca ggaaggccgc ggccgtgggc
     2581 ggagagccgg cccgggcatc gagcagggta atcctggtct cccgctgcga gtaagcagtg
     2641 aagatgaccg gcaaatacgc aatcacgacg gccaggaagc tgaagcccgt cgctccctcg
     2701 gtgaccgcca gaaatcggcc gagccccgct ttcggagcca ggtcgccgta gccgatcgtg
     2761 aagaacgtct cgccgctcag gtagaggacc gcgtccaaac cgggatgcgg atccggccca
     2821 ccgaggggcg tgccggtccc ccagatcaag aggccgaacc cggtcacgag cccggcggcc
     2881 caggtgccca gcagcaggag cagggccaag ggaccgaacc cgccgagaaa tccctcccga
     2941 agcccacctc cttcgcgatt tccggagctt ggcaggagcc gggccgcgcg ggcccagatg
     3001 cgccagccgt tgagcacgat cagccgggtc aaccggaagc ggcgggttac ccgccggggc
     3061 aggatcatgg tctcgaaggc atcgatcagg atcacgccga tcaggccgat ccccaggatg
     3121 acggcgagac gtgtcacgcg ggcccggccg attccccgcg cagggcgtag gccagcttct
     3181 cggcaaccgc gaccaccgtc gcgtggatgt tggcgcgcgg actggtcgga aagacggacg
     3241 catcaaccac tcgcaatccc cgaacgccgt gcacccgacc cgccagatcc accacggccc
     3301 ccggatcatc ggccggaccc atccggcagg tgctggacgg gtggtaggtg gtgtccgcgt
     3361 gggccgggac cagctcccgc aactcctccc ggctcgacca tgccagcgag gcccgcgcgg
     3421 gagacaggat ctcggccagc ggcggtgtcg ccgccagccg acgggccagc tcgatcccgt
     3481 cgcagatcgc ctccagatcg gccggatcgg cgaagtggtt gtggtcgatc gcgagtgtcg
     3541 cattcgggtc cggggacgtc aggcggacag tcccctgcga ccgcgccacc atcaggctgg
     3601 tcggcaggac gaagacccag ccgccggcat ccgggtcgtt gttgaccatc gagaagagga
     3661 aatagtcgat cccgtcagcc aattgccggc tccgaccctt gatcaccagc cggatgaagg
     3721 cctgtcgatc cgggacagca ttcgggtgga cgaggtgcag gcagaagccg tttgcggcga
     3781 gcgggtgatc gagcaggttc gccccgacgc ccggcagatc ccgcgcgacc ggaattccga
     3841 actcgcgcag gtgatcggcc gggccgatcc cggaccgcat gaggatcgcc ggcgatccgt
     3901 acgcgccagc cgccagcaca gtctcccgcc cggcaaagat ccggccgtcg actgtctgta
     3961 ccgccgtcgc gcgaccgtcc gtaatcgcga cacaatcgac tggcgcaccc gggatgatct
     4021 cgaggttcgg gcgattccgc gcggcggcca ggtaggtcag cgcgccattc atgcgtacgt
     4081 tttcgaccac gttgcgcggt gccccgctga tgcagacgtc ctgcctgtcc ggcaggtttt
     4141 ggttgtcgac ccacgggatc ccggaggccc cggccgcgtc gatcagggcg cgttcaacct
     4201 cgttcggctg cccgtcgtca agccgccgga ttggcaccgg tccggtcgtg ccctcgagcg
     4261 ggtcgttctc ggctttccgg aaccagggcg cgaggtcggc gtagccccag ccggggttgc
     4321 ccatcgccgc ccacgcgtcg tagtcggccg gcgcgcctcg cagccagacc gaaccgttga
     4381 cggccgacgt gccgcccatc agccggc
//
LOCUS       DASSGX010000349         2774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1368832, whole genome shotgun sequence.
ACCESSION   DASSGX010000349 DASSGX010000000
VERSION     DASSGX010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1368832"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="VFI12_06185"
     CDS             complement(<1..816)
                     /locus_tag="VFI12_06185"
                     /inference="COORDINATES: protein motif:HMM:NF023354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-dependent oxidative N-demethylase subunit
                     alpha family protein"
                     /protein_id="HET7056031.1"
                     /translation="MLGQLPPPYQPFLVRDDLIVRADLFPMPGTYNGRREALHFELDN
                     QVCAFLEEKLAALERAPEQYRCLDRSEAGALAEVYWRMFQILAREHPDFVQIDKSTVE
                     LRLLGLSLDLSDPQRLGIAIQPGAPNIGRRIAEWIEAQSGIDRLGDALALACQEDIVI
                     MRTLDDGRHLAESLHVMLPSAWSPLEKYRQSFGTIHEPVADSARLVASANNVIKAMVT
                     RGPYVRFGLSLSTLPRLDNHPDHAKPWNPTWLDDPDLLASKVSVRIERQTTFPM"
     gene            complement(830..1693)
                     /locus_tag="VFI12_06190"
     CDS             complement(830..1693)
                     /locus_tag="VFI12_06190"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET7056032.1"
                     /translation="MKLLTFKADGVLKSGLVTDKGVIDVAAASLALGIDAPGTVAGLA
                     DGLGKLQELASKVQGGEHWLHDEASLTLGPCVSDPGKIVCVGLNYAKHAAESGMPIPQ
                     FPVLFSKFNNALAGNGEDVPLIPDVASEFDYEVELCVAIGKTARNVSEEDALSYVAGY
                     CTANDLSVRDLQMRTSQWLLGKTSDKFFPIGPYLVTADEVGDPQKLRLTCKVNGETRQ
                     DSNTADMVFSVAQLVSYCSTYFTLEPGDLITTGTPEGVAMGMPNKPWLKKGDVVEVEV
                     EKLGKLTNTMA"
     gene            complement(1764..>2774)
                     /locus_tag="VFI12_06195"
     CDS             complement(1764..>2774)
                     /locus_tag="VFI12_06195"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7056033.1"
                     /translation="FEATSAGATRYTQSGGVLPLRQAIADRYAVKWGKEVTPNMVMAS
                     HGGVNAINSTIFALIDRGDEVLVPDPGWPNYRQILNLVGGVSVPYPMSVENGYLPDPE
                     ALERLVTPKTKMIIICNPGNPTGAVWPEATVNAILDLAKKHDLFVLSDEIYEELVFDG
                     VHVPAAKFDDDGRVITVSGFSKTYAMTGWRLGWAIADVSLIQMINKMLEPLISCPSSV
                     SQQAGIAALTGTQQPVAVMCNAYRKRRDLAAELLEPAGLLPVRPQGAFYALVDLRETG
                     MNGRDLAMALLKEKRVATAPGDTFGKVLADGFVRISLASSDEDVAEGCRRLIAFRDEH
                     RQ"
BASE COUNT          477 a          902 c          879 g          516 t
ORIGIN      
        1 catcgggaac gtcgtctgcc gctcgattcg gacgctgacc ttgctcgcca gcaggtcggg
       61 atcgtcgagc caggttgggt tccacggttt cgcatggtcc gggtggttgt ccagcctcgg
      121 cagcgttgac agggagagac cgaagcgcac gtatggtccc ctggtgacca tcgcctttat
      181 gacgttgttt gccgatgcga ccagtcgggc gctgtccgcc actggttcgt ggatcgtccc
      241 gaacgactgc cggtactttt caagcggact ccacgcgctc ggcagcatta cgtgaagcga
      301 ctccgccaga tgccgtccgt catcgagcgt ccgcatgatc acgatgtctt cctggcaggc
      361 gagcgcgagc gcgtcgccga ggcggtcgat tcctgattgc gcttcgatcc actctgctat
      421 gcgccgcccg atgttcggtg caccgggctg gatcgcgatg ccgaggcgtt gcgggtcgct
      481 caggtcgagc gacagcccaa gcaaccgcag ctccacagtc gacttatcga tctgcacgaa
      541 gtccgggtgc tcgcgcgcca ggatctggaa catccgccag tacacttcgg cgagcgcccc
      601 ggcctccgat cggtcgaggc agcggtactg ctccggcgct cgctcaagcg ctgccagctt
      661 ctcttcgaga aatgcgcaca cctgattgtc gagttcgaag tgcagcgcct cccgccgccc
      721 gttgtacgtg ccgggcatcg ggaagagatc cgcgcggacg atcaggtcat cgcggaccag
      781 gaacggctga tacggcggcg gaagctgacc gagcacgctg gatgacggat caggccatcg
      841 tattggtcag cttgcccagc ttctcgactt ccacctcgac gacgtcgccc ttcttgagcc
      901 acggcttgtt aggcatgccc atcgcgacgc cctcgggcgt gccggtcgtg atcaagtcgc
      961 ccggctcgag ggtgaagtat gtgctgcagt agctgacgag ctgcgcaacc gagaagacca
     1021 tgtctgcggt gttggagtcc tgccgggttt cgccgttgac tttgcaggtc aggcgaagtt
     1081 tctgggggtc gccgacctcg tcggccgtga ccaggtacgg gccgatcggg aagaatttgt
     1141 ccgacgtctt gccgagcagc cactggctgg tccgcatctg caaatcgcgc acactcaggt
     1201 cgttcgctgt gcagtaaccg gcgacatagg agagcgcatc ttcctcggag acgtttcgcg
     1261 cggtcttgcc gatcgccacg cagagctcca cctcatagtc gaactcgctc gcaacatcgg
     1321 gaatcagcgg tacgtcctcg ccattgccag ccagcgcgtt gttgaacttg gagaacagga
     1381 ccgggaactg cggaatcggc atgcccgact cggccgcgtg cttggcgtag ttcaggccga
     1441 cgcagacgat cttgcccgga tccgagacac acggcccgag cgtgaggctc gcctcgtcat
     1501 ggagccagtg ctcgccgccc tgtactttgc tcgcaagctc ctgcagcttg cccaagccat
     1561 cggcaaggcc ggcgaccgta cccggggcat cgatgccgag cgcgaggctg gcagccgcga
     1621 cgtcgatcac gcccttgtcg gtgaccaagc cggacttgag tacgccgtca gccttgaacg
     1681 tcagtagttt catgagatcc gcgtgtcctc tccctggaaa tcccgactcc aaaccagtcg
     1741 gtagcctacc aaagggcgtc cgcctattgg cgatgctcgt cccggaatgc gatcaaccgg
     1801 cgacacccct cggccacgtc ctcgtctgac gaggcgagcg agatccgcac gaatccatcg
     1861 gccagcacct taccgaaggt gtcgccgggc gccgtggcga cccgtttctc cttgagcagc
     1921 gccatcgcca ggtcgcgccc attcatgccc gtctcgcgca gatccaccag cgcgtagaag
     1981 gctccctgcg gacggaccgg caacaaaccg gccggctcga ggagttcggc cgccaggtcc
     2041 cgtcgcttcc ggtaggcatt gcacatcacc gcgaccggct gctgcgtgcc ggtcagggcg
     2101 gcgataccgg cctgctgcga gacggatgac gggcaggaga ttagcggctc gagcatcttg
     2161 ttgatcatct ggatcaggga gacatcggcg atcgcccacc cgagtcgcca gccggtcatc
     2221 gcgtaggtct tcgaaaagcc cgagaccgtg atcacccggc cgtcatcgtc gaacttcgca
     2281 gccgggacat gcacgccgtc gaagaccagc tcctcgtaga tctcgtcgga gaggacgaag
     2341 agatcgtgct tcttcgcgag gtcgaggatc gcattgaccg tcgcctccgg ccagactgcg
     2401 ccggtcggat tgcccgggtt gcagatgatg atcatcttcg tcttcggcgt gaccaggcgc
     2461 tcgagggctt cgggatcggg cagatagccg ttctcgaccg acatcgggta cgggacggag
     2521 acgccgccga cgagattcag gatctggcga taattcggcc aacccggatc cggaaccagc
     2581 acctcgtcac cgcggtcgat cagggcgaag atcgtcgagt tgatcgcgtt cacgccgccg
     2641 tggctggcca tcaccatgtt cggcgtgacc tctttgcccc acttgaccgc gtagcgatcg
     2701 gcgatcgcct gccggagggg gaggacgcca ccgctctggg tgtaccgggt cgcgccggcg
     2761 gaggtcgcct cgaa
//
LOCUS       DASSGX010000350         2283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1041355, whole genome shotgun sequence.
ACCESSION   DASSGX010000350 DASSGX010000000
VERSION     DASSGX010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1041355"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..1128
                     /locus_tag="VFI12_06200"
     CDS             67..1128
                     /locus_tag="VFI12_06200"
                     /inference="COORDINATES: protein motif:HMM:NF024755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HET7056034.1"
                     /translation="MPRDAAELAHRLAREAEAVCRHYLSNGRREGRYWLVGDARNTPG
                     RSMFVRLKESPKGPAGKWTDAATGEHGDLLDVIRESRGLIDFHDVADEARRFLSLPRI
                     ELEPPPKHPRPPAPIGSPDAARRLFAMAQPVTRTLAETYLRNRGITPLHEDGALRFHP
                     RCYYRPDEHSPTETWPAMVARVTDLDGRITGAHRTWLDPDGFDATRLGKAPIDTPRRA
                     MGDLLGNAVRFGVANEVLAAGEGIETMLSLRCVLPTLPMAAALSANHLAALLLPPTLR
                     RLYIARDSDAAGAMAATALTERAEATGVETIVWTPRRGDFNEDLRGFGLDALRAALRV
                     QLVPQDVARFMLLMAAETG"
     gene            1580..>2283
                     /locus_tag="VFI12_06205"
     CDS             1580..>2283
                     /locus_tag="VFI12_06205"
                     /inference="COORDINATES: protein motif:HMM:NF022151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2493 domain-containing protein"
                     /protein_id="HET7056035.1"
                     /translation="MAGAVADIFDALVSTLNDTRLEPDLEDLLWSTVNLFHRAVDRIE
                     RELDDNEQAQHRSQREQNGSEVRSVELERLTAEGLTLTERRNCMELFRDQAAERFEVH
                     TGTPWRPRSGSLVNHRRLTAAMIDSRDFLAAKRRAETEVMLPPGPKIALTGGLDFNDH
                     RLIWDRLDKVHAKHPDMVLLHGGSPKGAELIAAKWASNRKVPQIAFKPDWTKHAKAAP
                     FKRNDAMLAVLPIGVL"
BASE COUNT          392 a          833 c          713 g          345 t
ORIGIN      
        1 cgctgtcctg gcgcgggtac tcgaacgcta tcccgtcgag cgcgtcggca aacgggaggc
       61 cgcgtgatgc cacgcgacgc cgccgaactg gcgcatcgcc tcgcgcgtga ggccgaggcg
      121 gtgtgccggc actatctctc caatggccgg cgagaaggcc gctactggct ggtgggcgac
      181 gcgcgcaaca cacctggacg ctccatgttc gtccgcctca aagagtcgcc gaaagggccg
      241 gccggcaaat ggaccgacgc cgccaccggc gagcacggcg atctgctgga cgtcattcgc
      301 gagagtcgtg gcctcatcga cttccacgat gtcgccgacg aagcccgacg ctttctgagc
      361 ctgccacgca tcgaattgga gcctccgccg aaacatccgc gacctcccgc gccgatcggc
      421 tcgccggacg cggcacgacg gctcttcgct atggcccagc cggtgacgcg cacacttgca
      481 gagacgtatc tccgtaatcg cggcattacg cctttgcacg aagacggcgc actgcgcttc
      541 catccgcgct gctattaccg gcccgacgag cattcaccga cggagacctg gccggcgatg
      601 gtcgcccggg tgaccgatct cgacggccgg atcaccggcg cgcaccggac ctggctcgat
      661 cccgacggtt tcgacgccac ccgtctcggc aaagcgccga tcgacacgcc gcgacgcgcc
      721 atgggcgacc tcctcggcaa cgccgtccgc ttcggcgtgg cgaacgaggt gctcgccgcc
      781 ggcgaaggca ttgagaccat gctgtcgttg cgctgcgtgc tgccaaccct gccaatggcc
      841 gccgcgctct cggccaatca cctcgcagcc cttctgctcc cgccgacgct gcgccgcctt
      901 tacatcgctc gcgacagtga tgcggccggc gcaatggccg cgacggctct gaccgaacgc
      961 gccgaggcga ccggcgtcga gacgatcgta tggacgccca gacgtggcga cttcaacgag
     1021 gatctgcgcg gcttcggcct cgatgcactt cgggcagcct tgcgggtcca gctcgtgcca
     1081 caggacgtcg cccgcttcat gctcctgatg gcggccgaga cgggctgaag cagcagcagt
     1141 tcgggtgtcg ccatagccgg tcgggcgagg ccgaacagcc ggacagagtc cgcgccctgg
     1201 ccttctagag ggcgatcgga cggcagccgg cccggcccgg caatggctgc gcccggctat
     1261 tttccgccgg ccctctcggg cctttgcatc gcgaagcaaa atagccgggc ttcgccatcc
     1321 tccgctgacg cttcggccct gcgctgcgct ccgggttctg ggcagtgccg gccgccgtct
     1381 ggtgatcgcc acgaaggccg cgatgggcgc ggccgatccg gcaaagggcc tcaccatgac
     1441 gaccgaccac gacgacaccg atttcgaacc gccgcacagc tcctctccga ccgatcacgt
     1501 cctcaacgag cttcagctct atggctaccg tcccttccag gacgagcccg atccgaggcc
     1561 gctccccgaa ggcaacgcgg tggccggcgc cgtcgcagac atcttcgacg ccctggtctc
     1621 gaccctgaac gacacgcgtc tcgaaccgga cctcgaggat ctgctctggt cgaccgtcaa
     1681 tctcttccac cgcgccgtcg accgcatcga acgcgagctc gacgacaacg agcaggcaca
     1741 gcaccggagc caacgcgagc agaatggctc cgaggtgcga tcggtcgagc tcgagcgatt
     1801 gacggccgaa ggtctcacac tgaccgagcg ccgtaactgc atggagctct tccgcgacca
     1861 ggccgccgag cgcttcgaag tccacaccgg cacgccctgg cgtccccgct cgggatcgct
     1921 ggtgaaccat cgcaggctga ccgccgcgat gatcgacagc cgcgacttcc tggccgccaa
     1981 gcgccgcgcc gagacggaag tgatgctgcc gcccgggcct aagatcgcgc tcaccggcgg
     2041 gctcgacttc aacgatcatc gcctgatctg ggatcggctg gacaaggtcc acgccaagca
     2101 tcccgacatg gtgttgctgc acggcggctc gccgaaaggc gccgaactca tcgctgccaa
     2161 atgggcgagc aaccgcaagg tgccgcagat cgccttcaag cccgactgga cgaagcacgc
     2221 gaaagccgct ccattcaaac gcaacgacgc gatgctcgcg gtgttgccga tcggcgtcct
     2281 tca
//
LOCUS       DASSGX010000351         3523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1587335, whole genome shotgun sequence.
ACCESSION   DASSGX010000351 DASSGX010000000
VERSION     DASSGX010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1587335"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VFI12_06210"
     CDS             complement(<1..478)
                     /locus_tag="VFI12_06210"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7056036.1"
                     /translation="MTTSIQQTMTRPEPVAPAPLLEVRSVTKAFGGNLAVNDCSFTVG
                     HGTITGLIGPNGAGKSTLLNIIAGALRADGGSILLDGEPITGLGPHMLLRRGLARTFQ
                     VPRPIPTMTVLENLVLAGPAQPGERIWNTWFRPGQVGRREDAIIDEALTVLDYVNLL"
     gene            complement(610..1950)
                     /locus_tag="VFI12_06215"
     CDS             complement(610..1950)
                     /locus_tag="VFI12_06215"
                     /inference="COORDINATES: protein motif:HMM:NF013275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7056037.1"
                     /translation="MVGRKGRDVKSASRGGPGRWGWRVLAALLLVIPLVTALGGRGAV
                     AQDDVAPLRIGTLFPFTGDLSDFGPAFWNAAELAANQINAGGGVNGKPIELVRADTAT
                     SPQQAVEEARRLVDVEGVPAMIGPAGSGEALPVAESIAGPSHVLVISPSATSPALSIA
                     NDDDFFFRTTISDAAQGVVMADLAHEQGYTTACVFYLNNAYGQGLSDAFAKQFTSDGG
                     TVTAQVPHEGEQASYASELATCTADNPDVMIAISYPESGRIFLRELVESGNVPALIFS
                     DGLKSSDLFQELGWEQFEGDYGTAPGSAETEIGKAFDQAWQEAYGALPSYPYLRETYD
                     AVYVIALAAQQAHSVDATGIRDALRGVANAPGTVVTPGTEGWKKAVAALDAGEDIDYE
                     GAVGPLNFDQNGDVDKGTIVIWQIKNGEIVTAETREIDLSGAAPSGTPAATPAA"
     gene            2633..3022
                     /locus_tag="VFI12_06220"
     CDS             2633..3022
                     /locus_tag="VFI12_06220"
                     /inference="COORDINATES: protein motif:HMM:NF017880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HET7056038.1"
                     /translation="MANQRIPGPAPAGVTLHLTPEPVWRAQRSLPEYRPEGFAAGGFI
                     HNTDGETEVLAVANRYYRGDARPYLLLDVDLARVTAPAIYEDAARRYPHIYGPIPLDA
                     VLRVRRVERAPDGAFTAIGDVLEDAGP"
     gene            3137..>3523
                     /locus_tag="VFI12_06225"
     CDS             3137..>3523
                     /locus_tag="VFI12_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056039.1"
                     /translation="MFALLGRMAYRWRRAILIAWGVALLLALPTLPRVSGELKVGGFS
                     SPHTESARASEVLQHELGFAPSTLLVIYQSETMRATDPAFQNAVGRSLAGVRTLPNVT
                     GIILPSVDASLISRDGDTAYAIVGVGR"
BASE COUNT          572 a         1172 c         1200 g          579 t
ORIGIN      
        1 ggagcagatt gacgtagtcg agcacggtga gcgcttcgtc gatgatggcg tcctcgcgac
       61 gaccgacctg gccaggccga aaccaggtat tccagatccg ctctcccggc tgcgccggac
      121 cagccaggac gagattctcc aggacggtca tggtcgggat cgggcgcggc acctgaaacg
      181 tgcgcgcgag cccgcggcga aggagcatgt gggggccgag cccggtgatc ggttcgccat
      241 cgaggaggat cgacccaccg tcggcgcgca gggcgccggc aataatgttg aggagggtcg
      301 acttgccggc gccgttgggg ccgatcaggc cggtgatcgt accgtgcccg acggtgaacg
      361 aacaatcatt gaccgccagg ttgccgccga acgccttggt caccgaccgc acctcgagca
      421 ggggcgcggg ggcgaccggc tcggggcggg tcatggtctg ctgaatcgag gtcgtcatgc
      481 tctccacaat gcagggctac gtcgtatctg gtctcgctgg accacctcca acccgaagag
      541 gagccgatcc gcgtgtgcga cggatcggct cccgtgacga ccaggtcgac gagcgaccgg
      601 ggtgccggct caggccgctg gtgtcgcggc cggagtgccg ctcggcgccg caccggacag
      661 gtcaatctcc cgtgtctccg cggtgacgat ctcgccgttc ttgatctgcc agattacgat
      721 cgtccccttg tcgacgtcgc cgttttgatc gaagttgagc ggtccgaccg cgccctcgta
      781 atcgatatcc tcgccggcat cgagcgcggc gactgccttc ttccaccctt ccgtgcccgg
      841 ggtgacgacc gtgcccggcg cgttcgcgac gccccgcagc gcgtcgcgga tccccgtcgc
      901 gtcgacagaa tgggcctgtt gcgcggcgag cgcgatcacg tagacggcgt cgtaggtctc
      961 gcgcagatag gggtaggacg ggagcgcacc gtaggcctcc tgccaggctt ggtcaaaggc
     1021 cttgccaatc tccgtctctg ccgaaccggg tgcggtaccg tagtcgccct cgaactgctc
     1081 ccacccgagc tcctggaaga gatcggacga cttcagaccg tcgctgaaga tcagggccgg
     1141 cacgttcccg gactcgacca actcacgcaa gaagatgcga ccgctctccg ggtaggagat
     1201 ggcaatcatg acgtccggat tgtccgccgt gcaggtggcc agctccgagg cgtagcttgc
     1261 ctgctccccc tcgtgcggca cctgcgcggt gacggtgccc ccatccgaag tgaactgttt
     1321 ggcgaaggcg tcgctcaggc cctggccgta ggcgttgttg aggtagaaga cacaggccgt
     1381 cgtgtacccc tgctcgtggg cgagatcggc catgaccacg ccctgggcgg cgtcggagat
     1441 cgttgtgcgg aagaagaagt cgtcgtcgtt cgcgatcgac aacgctggcg aggtggccga
     1501 cggggagatg acgagcacgt gggagggtcc ggcgatagac tcggccacgg gcagagcctc
     1561 gccgctgcca gccggcccga tcatggccgg caccccttcg acgtcgacca ggcggcgggc
     1621 ttcctcgacg gcctgctgcg gcgaggtcgc ggtatccgcc cggaccagct cgatcggctt
     1681 gccgttgacg ccgccgccgg cgttgatctg attcgccgcc agttcggcgg cattccagaa
     1741 cgcgggaccg aagtcagaga ggtcaccggt gaaggggaag agcgtgccga tgcgcagcgg
     1801 cgccacgtca tcctgggcga cggcgccgcg gccgccaagt gcggtgacga gaggaatgac
     1861 cagcaggagc gcagcgagga cgcgccaccc ccaacgcccg ggtccgcccc tgctggcgga
     1921 tttcacgtcg cggcctttcc gaccaaccat cacaccctcc tcgatcgtga ccgggacgct
     1981 acccattccg cgccccggca aataacgctc gcacgggctc gccgttcgca atcgcttcct
     2041 caagtcgcag aaaaacacag aaatccgggg agtgaaaccc tctccggagg gtcgcctgga
     2101 tcgggctccg cctcacgatc ggatcgcccg atccagccga gttccgaacc gccgaatgta
     2161 ccgaacaggc taccggcagg ggctgcgccg agtcaaggaa tgcttgcagt ttggcgcgga
     2221 atctggcctg acaccctccg cgaaacacga gcgaccgggc gaattggatc ggtcgttcga
     2281 cgctgagtgc tcgcggtagc gattcgccct cctggcgcaa ccacgagccc cgttccacgg
     2341 ggcttgcctt ttgagttagc cgggtgtctt gagcgcccgg cgcagcggcg gaggcggtcg
     2401 aggtggcaac ggcccttgtc tccatccgcc gccgcctcgt ctcgccagcg gctaaagacc
     2461 gctggctgcc tgaacgaaag ccctgtggaa cggggctgaa gaatgcgctg tcctcgagct
     2521 tgaggggtgg cttggtgaag ctcgggcggg cggcatgtcg ccccgcggat catggatagt
     2581 tccctcggta gcgcatggca ctgtgcattc aagcagaaca ctggaggcag cattggcgaa
     2641 tcagcgcatc ccgggacccg cgccggcagg tgtcaccctc catctgacgc cggagccggt
     2701 ctggcgcgcg cagcggtccc tgccggagta ccggccggaa gggttcgccg ccggggggtt
     2761 catccacaac accgatggcg aaaccgaggt gctcgcggtg gcgaaccgct actatcgcgg
     2821 tgatgcgcgg ccctacctcc tcctcgatgt cgatctcgcg cgggtgacag cgccagcgat
     2881 ttatgaggac gcggcgcggc gctacccgca catctacggt ccgatcccgc tcgatgcggt
     2941 gctacgcgtg cggcgggtgg agcgagcacc ggatggcgcc tttacggcga tcggcgacgt
     3001 gctggaggac gccggcccgt agccgccgcc ggcactgcat tcccgcgagc ggcgatcatt
     3061 gccgctgtgg gccgcgcatc gcgccgcgcg gcgcctggct agaggaccga cgagatacga
     3121 ggaccggact tcatcggtgt ttgcactact cggcaggatg gcgtaccgct ggcggcgagc
     3181 catcctgatt gcctggggcg tggcgctcct gctcgcgctg ccaacactgc cgcgggtgag
     3241 cggcgaactg aaagttggcg gcttctcgtc tccgcatacg gaatcggcgc gcgcgagcga
     3301 ggtgctccag cacgagcttg gcttcgcacc gtcgacgctc ctcgtcatct accagtccga
     3361 gacgatgcgg gcgaccgacc cagcgttcca gaatgctgtg ggccggtcgc tggccggcgt
     3421 ccgcaccttg ccaaatgtca ccggcatcat cctgccctcg gtcgatgcca gtctcatctc
     3481 ccgcgacggc gataccgcct acgccatcgt cggggtgggg cgg
//
LOCUS       DASSGX010000352         2193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_934280, whole genome shotgun sequence.
ACCESSION   DASSGX010000352 DASSGX010000000
VERSION     DASSGX010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934280"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /gene="ftsY"
                     /locus_tag="VFI12_06230"
     CDS             complement(<1..855)
                     /gene="ftsY"
                     /locus_tag="VFI12_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HET7056040.1"
                     /translation="MSKGLEKTRRGVFSEIARLFDRGAFGEGFFDELEMLLIQADLGW
                     DISQQLVEDLRASVEQHAITDPQFAKDYLRSDMVRLLELATRNRKVKILQRGVPFVIM
                     VVGVNGVGKTTSIAKLAAYHQRFGRSVMLAAGDTFRAAAIDQLNVWGDRLDVPVIAQT
                     QGSDPGAVVFDAMQAAHNRNIDVLIVDTAGRLHNKSNLMAELTKIRKIMQRHVEDAPQ
                     EVLLVIDATTGQNGLVQAKEFAKSVDITDIMIAKLDGTAKGGIAFSVAKELGTPISYI
                     GTGEKATDL"
     gene            1031..1723
                     /gene="radC"
                     /locus_tag="VFI12_06235"
     CDS             1031..1723
                     /gene="radC"
                     /locus_tag="VFI12_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HET7056041.1"
                     /translation="MLAEMPEAERPRERLLRHGPSALTNVELLAILLNTGVVGESVMT
                     VAERLLREHGGFPGLMKLSADQLSRLHGLGPAKATKLKASMEIANRILASNPDQKPRI
                     ASPDDITNLIGLEMSLLEQEQLRVVLLNTRNEVIGIRTLYQGTTNQAPVRIGEVFREA
                     VRANAVAIIVVHNHPTGDPTPSSADIELTRELVSAGKTLDVPLLDHMIIGHGRHVSMK
                     RLGLGFTSDYGR"
     gene            1730..>2193
                     /locus_tag="VFI12_06240"
     CDS             1730..>2193
                     /locus_tag="VFI12_06240"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET7056042.1"
                     /translation="MERGYGVSALVSPDLIAPLAQAAEADGYQTFWVNDVPGGNGLEQ
                     LAQAQAVTSGIRLGVGVLPVDRWSAEQIAAEVHRLRLDPERLLLGIGAGAMHKGSLDA
                     IGNLASELIRLVPTRVLIGALGPKMCRLAGSAAHGVILNWLTPDAAADLALQT"
BASE COUNT          403 a          681 c          694 g          415 t
ORIGIN      
        1 gagatctgtc gctttttcgc ccgttccgat ataggagatc ggcgtgccga gctccttggc
       61 gaccgagaaa gcgatgccgc ccttggccgt gccgtccagc ttggcgatca tgatatcggt
      121 gatatcgacc gacttcgcga actccttcgc ctggacgagg ccgttctggc cggtcgtcgc
      181 gtcgatcacg aggagcactt cctgcggcgc gtcctcgaca tgccgttgca tgatcttgcg
      241 gatcttggtt agctcggcca tcaggttcga cttgttgtgc agccgtcctg ccgtgtctac
      301 gatcaaaacg tcgatgttac ggttgtgcgc ggcctgcatc gcgtcgaaga caaccgcgcc
      361 gggatcggat ccctgcgtct gagcgatcac cggcacgtcc agccgatcgc cccagacgtt
      421 gagctggtcg atcgccgcgg cccggaacgt gtcgcccgcc gccagcatca ctgagcggcc
      481 gaagcgctgg tggtaagcgg ccagcttcgc aatcgacgtc gtcttgccga caccgttcac
      541 gcccacgacc atgatcacga aggggacacc gcgctgcagg atcttgacct tgcgattgcg
      601 cgtcgctagc tccagcaagc ggaccatgtc gctccggagg tagtccttcg cgaactgcgg
      661 gtcagtgatg gcgtgctgct cgaccgatgc cctcaaatcc tcgacaagtt gctggctgat
      721 atcccagccg aggtcggcct ggatcaggag catttccagc tcgtcgaaga agccctctcc
      781 gaacgctccg cgatcgaaca gtcgagcgat ctcgctgaag acgccgcgcc gggtcttttc
      841 cagacccttg ctcaattgct gatcaggttc ggtttgacgt cggaaaagac ggctgaagac
      901 cacagtgcta tgcctctgtc gaaggtgagt cagatgcgag gatggctcga gcgccggttc
      961 accccaaagt atatcatcgg cacgattctg cccggcatgc gatcatcttt tcgtgaccga
     1021 acgacgacat atgttggcgg agatgccgga ggctgagcga ccgcgcgagc gactgctccg
     1081 gcacggcccc tccgcgctga caaacgtcga gctgctcgcg atcctactca acactggcgt
     1141 cgtcggggaa tcggtgatga ccgtggctga gcggttgctg cgcgaacacg gcggcttccc
     1201 cggcctcatg aagttgagcg ccgaccagct gtcccggctg cacgggcttg gaccggccaa
     1261 agcgacgaag ctcaaggcat cgatggagat cgcgaaccgc atcctggcat cgaacccgga
     1321 ccagaagccg cgcattgcca gcccggacga cattacgaac ctcattggac tcgaaatgtc
     1381 gctgctcgag caggagcagc tacgggtcgt actgctcaac acgcggaatg aggtgatcgg
     1441 cattcggacg ctctatcagg ggacgacgaa ccaggcgccg gttcggatcg gtgaagtatt
     1501 ccgtgaggcg gtccgggcca atgccgtcgc gatcatcgtc gttcataatc atccgaccgg
     1561 cgacccgacg ccctccagcg ccgatatcga gctgacgcga gagctggtca gtgccggcaa
     1621 gacgctcgac gtgccgctcc ttgaccacat gatcatcgga catggccggc acgtctccat
     1681 gaagcgactc gggctcggtt tcacgagcga ctacggacgg taggacgaca tggaacgggg
     1741 atacggcgtt tcggctctcg tcagtccgga tctgatcgcg cccctcgcgc aggcggcgga
     1801 ggcagacgga tatcagacgt tctgggtcaa tgacgttccg ggcggaaacg ggctcgagca
     1861 gcttgcccag gcgcaggccg taacgtcagg tatccggctc ggagtgggcg tgttaccggt
     1921 cgatcgctgg tcggccgagc agatcgcggc tgaggtccat cggctcagac tggacccgga
     1981 acggttgctg ctcggcattg gtgccggcgc aatgcacaag gggtcccttg atgcgatcgg
     2041 gaatctcgcc agcgaactga ttcgcctcgt tccaacacga gtgttgatcg gcgcgctcgg
     2101 gccaaagatg tgccgcctgg caggttcggc cgctcacggc gtgatcctga attggctgac
     2161 cccggacgcg gcggcagatc tggcgctgca gac
//
LOCUS       DASSGX010000353         2514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_170254, whole genome shotgun sequence.
ACCESSION   DASSGX010000353 DASSGX010000000
VERSION     DASSGX010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170254"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(275..616)
                     /locus_tag="VFI12_06245"
     CDS             complement(275..616)
                     /locus_tag="VFI12_06245"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HET7056043.1"
                     /translation="MSEDQPYLQHIVDAITQIEEYVTVGRDRFVTEHHWQDSVIFQLI
                     IIGEATKHLSADLRVRHPEVAWRSMAAMRDVLVHNYMGIDLNVVWDVAVRMLPEVKRG
                     VESILDEESRG"
     gene            complement(613..1320)
                     /locus_tag="VFI12_06250"
     CDS             complement(613..1320)
                     /locus_tag="VFI12_06250"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HET7056044.1"
                     /translation="MQKNLRPCKRRILPDMDQTIVDRDIARLLHIRDAIARIEEYAAA
                     GHEQFLVESMTQAAILRQLEIVCEAAKRLSPELRRRHAEFPWGRLRVLQDEFDHHFLE
                     LDLNLVWDVTLETIPHLKRMVEAVLDEAAPAEDTGSVMAIDALLIDNRAEILRIAKEH
                     GALSVRIFGSFAKGTAGPNSDVDVLVDLEPGRSLFDLIGIKHGIEDLLGRAVHIVTEE
                     AISPYLREDIIRSAAPL"
     gene            1319..1810
                     /locus_tag="VFI12_06255"
     CDS             1319..1810
                     /locus_tag="VFI12_06255"
                     /inference="COORDINATES: protein motif:HMM:NF003819.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HET7056045.1"
                     /translation="MASLSSFDIVSDFDQQEMKNALDQTQREIHTRYDLKDTKTTIEA
                     TDNQLTVNADTEYTLGTVRDLLESKLIRRGLSLKILDYQQEEPASGGRVRQVVKLRRG
                     VPEDLAKQLSKRIRDDFKKVTPQIQGDAVRVQAKNKDDLQAVIQALKAEDFPVALQFT
                     NYR"
     gene            1943..>2514
                     /locus_tag="VFI12_06260"
     CDS             1943..>2514
                     /locus_tag="VFI12_06260"
                     /inference="COORDINATES: protein motif:HMM:NF013112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HET7056046.1"
                     /translation="MTETPTRAADRAFHILTLGCSKNRVDSDGMDHLLRSRGMVAADR
                     PEDAQVVIVNTCGFLGAARAESVAAIGELIDRRQEGQVVIAAGCMPALGDYRNDIPTG
                     VDRVLTTREWFRIGDVVGDLLGEAPQPEVAGCEGMLTTFSRAQAGPSAYVKVADGCDH
                     ACAFCTIPSIKGRQASKRPLHVLQEIVDLV"
BASE COUNT          496 a          812 c          758 g          448 t
ORIGIN      
        1 gggcgccgag tcgccgtcga cccggcgtac gaaccaaagt tcacgatccg cgcctcgctg
       61 tcgtcatcct ccttcctgaa gcactttcgg acgagcatcc ccagcatcgc attggcctac
      121 cggaatccag gaaggcgacc ccagcacgac cgccgtcgtc acgccgtgcg gtccgaccaa
      181 ccgacacgat gcgcgccggc tcgccgggag ccgatgtgga agccctcgga tcatagcgca
      241 cggctcgggg acccgctaca ggattggaac caggtcagcc gcggctctcc tcgtcgagga
      301 tcgactcaac cccccgcttc acctcgggaa gcatgcgtac cgccacatcc cacacaacgt
      361 tgaggtcgat ccccatgtag ttgtgcacga gtacgtcccg catcgctgcc atgcttcgcc
      421 aagcgacttc gggatggcgc acgcggagat cggccgagag atgctttgtg gcctctccta
      481 tgatgatcaa ctggaagatg acggaatctt gccagtggtg ctcagtgacg aaccggtctc
      541 gtccgacagt gacgtattcc tcaatttggg tgattgcgtc gacgatgtgc tgaagatatg
      601 gctgatcttc gctcacaggg gggccgcgct gcggatgatg tcctctcgca aatacgggct
      661 gatcgcctct tcggtaacga tatggaccgc acgccccaag aggtcttcga tgccgtgctt
      721 gatcccgatg agatcgaaca ggctccggcc gggctcaaga tccacaagaa cgtcgacatc
      781 gctgttcggg cctgctgtgc ccttggcgaa cgagccgaag atccgcacgc tcaaagcgcc
      841 atgctctttc gcaatgcgaa ggatctcggc gcgattgtca atgagcaacg catcgattgc
      901 catcacggaa cccgtatctt cggcgggcgc cgcttcgtcg aggaccgctt cgaccattcg
      961 cttcaggtgc gggatcgtct ccaacgtgac atcccagacc agattgagat cgagttcgag
     1021 gaaatggtga tcgaactcat cttgcagcac ccgaagccgg ccccacggaa actccgcatg
     1081 cctcctgcgc agttccggtg aaagacgttt tgccgcctcg catacgatct cgagctgtcg
     1141 gaggatggcg gcttgcgtca tcgactccac caggaattgc tcatggccgg ccgcagcata
     1201 ctcctctatc cgggcgatcg cgtcccggat gtgcagaagt cgggcaatgt ctctatcgac
     1261 gatagtctga tccatatcgg gtagaatacg acgtttgcac ggacggaggt ttttctgcat
     1321 ggcgtcactc tcatcattcg acatcgtttc cgatttcgat cagcaagaaa tgaaaaacgc
     1381 gctcgatcag acccagcgcg agattcacac gcgctacgac ctgaaggaca ccaagacgac
     1441 gatcgaggcg acggacaacc aactgacggt caacgccgac accgagtaca ccctgggcac
     1501 ggtccgcgac ctgctggaat cgaagctcat ccggcgcggg ctctcgctca agatcctcga
     1561 ctaccagcag gaggagccgg cctccggcgg ccgcgtgcgc caggtcgtca agctgcggcg
     1621 cggcgtcccc gaagacctcg ccaagcagct cagcaagcgc atccgcgacg atttcaagaa
     1681 agtcaccccg cagatccagg gcgacgccgt ccgcgtccag gccaaaaaca aggacgacct
     1741 gcaagccgtg atccaggcgc tcaaggcgga ggatttcccg gtcgcgttgc agttcacgaa
     1801 ttaccgttga gggctatcgg ctaacagtcg ggaagtcggt gagtcggtca gtcggtaagg
     1861 acgagcaatc gccgacgaag agccgatagc cgatagccga tagccgatag ccggtagccc
     1921 actacccccg aggagcccca tcgtgaccga gaccccgact cgcgccgccg atcgcgcgtt
     1981 tcacattctg acccttggct gttcgaaaaa tcgcgtcgat tcggacggga tggatcatct
     2041 gctgcgctcg cgcgggatgg tcgccgccga tcggccggag gacgcccagg tcgtcatcgt
     2101 caacacttgc ggcttcctcg gtgcggctcg cgcggagtcg gtcgcggcga tcggcgagct
     2161 gatcgaccgc cggcaggagg ggcaggtcgt catcgccgcc ggctgcatgc cggccctcgg
     2221 ggactaccga aacgacatcc ctaccggcgt cgatcgcgtc ttgacgacgc gggagtggtt
     2281 tcgcatcggc gatgtggtcg gcgatctgct gggggaggcg ccgcagccgg aggtcgccgg
     2341 ctgcgagggg atgctgacca cctttagccg cgcgcaggcc ggcccctccg cctacgtcaa
     2401 ggttgccgat ggctgcgacc acgcctgcgc cttctgcacc atccccagca tcaagggacg
     2461 gcaagcgagc aagcggccgc tgcacgtcct gcaggagatc gtcgatctgg tcag
//
LOCUS       DASSGX010000354         4159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_825232, whole genome shotgun sequence.
ACCESSION   DASSGX010000354 DASSGX010000000
VERSION     DASSGX010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4159
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825232"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1238
                     /locus_tag="VFI12_06265"
     CDS             <1..1238
                     /locus_tag="VFI12_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056047.1"
                     /translation="LWRNLGLIAAGKKRYELMMVGGSYVGYIRSTRQWWAPVDRLLRE
                     QKLDQRPIYFVSSNMHSFANVLSGTARRRERELTEFVRTSTALGLADELVKIESGRTQ
                     SSLDNLLYFAARPYFALPEHVADRDRRAEEELERGIYHIEPRSSVDVGVQVIDLRRTD
                     PTAFDPRLCRSGEETLDPSATDAIIVNVNYPLGFAAYHLLSQVSMATEQMRGIYILGK
                     AATLNGRIGDVMLANVVFDEHSGNTYWFDNCFGYQDVAPFLLFGAALDNQKAVTVKGT
                     YLQNQGYLDFYYRENFTVVEMEAGPYISAIYEDSRLERHPSEQAINLRGAIGHDIDLG
                     IIHYASDTPYTRAQTLGARGMSFFGMDSTYASTIAILRRIFQKTGMLGSRAGEKPGAE
                     SRRREGAKLDEATRRKGNL"
     gene            complement(1494..2051)
                     /locus_tag="VFI12_06270"
     CDS             complement(1494..2051)
                     /locus_tag="VFI12_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056048.1"
                     /translation="MSAPFDGTDEHAIREPGQSHWHRPIGRRNVIAGIAGLGIAAGIG
                     VTQVVAQDATPSATEETGAGTDQTTREAKVGAAYQDFIGKLTTNLGESDPAKVDTAIR
                     DALKAMVDEQFDAGTISANLRDDLKSRIDSSDAPLGVAMVAGVRMHRMEGRRERRKDR
                     KHDDQSGEDDGGSTAQPSTTATPTI"
     gene            2210..2875
                     /locus_tag="VFI12_06275"
     CDS             2210..2875
                     /locus_tag="VFI12_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7056049.1"
                     /translation="MRILLVEDDEALADVIARGLRQAGLAVDIAPDGESAIESASINE
                     YDVVVLDRQLPGIHGDDVCRALLRRDAPPRILMLTAAGNLDDRVRGLEIGADDYLPKP
                     FAMAELIARIQALGRRPSAVRLPVIEIGDLQIDSNRRAARRGHHDLALTRKEFGVLEI
                     LAAEPGRVVSSEELLEKVWDEAADPFTNAVRITIMTLRRKLGEPPLIETVVGVGYRLS
                     EPA"
     gene            2872..4014
                     /locus_tag="VFI12_06280"
     CDS             2872..4014
                     /locus_tag="VFI12_06280"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7056050.1"
                     /translation="MTPFRPSVRLKLTLLYGALFLIAGLFLISVTYGLVRRELAPNAQ
                     RPISRVVQDRPGNHQDGPDDRVAGARREERRFALRQLWRQSVFALAIATVGALGLGWL
                     FAGQVLHPIRRITAHARSSSAATLDERIRLDGPEDELKELADTFDSMLDRLQAAFRAQ
                     QGFAAQASHELRTPLAIIRAEAELARDAPDASEQERQSADAILTAAGRSERLVDGLLA
                     LTRSESTMLQTAPVDLADLAGDVVGEYVREADQAGVRVDLALDAATVQGDPALLRQMI
                     GNLVQNAIRYNRPDGWVRVSVAPTDRAGRLVVENSGQLVGREEIDRIFEPFARGEWAR
                     RNRTGFGLGLAIVRTVAIAHHGTIQALAGPDGGLRITIDILLPLQQ"
BASE COUNT          710 a         1397 c         1363 g          689 t
ORIGIN      
        1 ggctctggcg caacctcggc ctgatcgcgg ccggcaaaaa gcggtatgag ctgatgatgg
       61 tcggcggcag ctatgtcggc tatatccgtt ccacgcggca gtggtgggcg cccgtcgacc
      121 ggttgctgcg cgagcagaag ctggatcagc ggccgatcta cttcgtctcc tcgaacatgc
      181 acagcttcgc caacgtgctc tccggcacag cacggcggcg ggagcgcgag ctgaccgagt
      241 tcgtgcggac gtcgaccgca cttggcctgg ccgacgaact cgtcaagatc gagtccggcc
      301 gcacccagtc ctccctcgac aacctgctct acttcgccgc caggccctat ttcgccttgc
      361 ccgagcacgt cgccgaccgg gaccgtcgcg cggaggaaga gctggagcgc ggcatctacc
      421 atatcgagcc ccgctcatcg gtcgatgtcg gtgtgcaggt gatcgacttg cggcggactg
      481 atccgaccgc attcgacccg cgcctctgcc gatctggcga agaaacgctc gatccttcgg
      541 caaccgacgc gatcatcgtc aatgtcaact acccgctcgg cttcgcggcc tatcacctgc
      601 tgtcgcaggt ctcgatggcg accgagcaga tgcgaggcat ttacatcctc ggcaaggccg
      661 caacccttaa cggccggatc ggtgacgtca tgctcgccaa cgtcgtcttt gacgagcact
      721 cgggcaatac ctactggttc gacaactgct tcggatacca ggacgttgcc cccttcctgc
      781 tcttcggcgc cgcgctcgac aaccagaagg ccgtcaccgt caagggcacc tacctccaga
      841 accagggcta tctcgacttc tactaccggg agaatttcac ggtcgtcgag atggaggccg
      901 gcccgtacat cagcgcgatc tacgaggaca gccgcctcga gcgccacccg agcgagcagg
      961 cgatcaacct gcgcggcgcg atcggtcacg acatcgatct cggcatcatc cactacgcct
     1021 cagacacgcc ctacacccgc gcccaaaccc tcggcgcccg cggcatgtcc ttcttcggca
     1081 tggactccac ctacgccagc accatcgcga tcctgcggcg gatcttccag aagacgggaa
     1141 tgctgggaag tcgagcaggc gagaagccag gcgcggagtc gcggaggcgc gaaggcgcga
     1201 agttggacga ggcgacgaga cgaaaaggca acctgtagag tcgcatggca atgcgacccc
     1261 agcctcggag aggcttcgtg ttctcagccc gggggttcaa cccagcgaat cgctcagaaa
     1321 acggaaaacc agagacgaaa gaccaaagac gaaagaccaa agacgagaga ccgccttcgc
     1381 gcctatgaaa acaacgaaac gggccggggc tagatcctcc cggtccgctt cgttgaagag
     1441 ggttggggcg gcccggccca cggggaatcg ggccgagccg gggtcggggc gtgctagatc
     1501 gtgggcgtgg cggtggttga cggctgcgcc gtcgagccgc cgtcgtcctc gccggattgg
     1561 tcgtcgtgct tgcggtcctt gcggcgttct cgccggccct ccatccggtg catccgaacg
     1621 ccggcgacca tggccacacc gagcggcgcg tcgctcgaat cgatgcgcga cttgaggtcg
     1681 tctcgcaggt tggcggagat ggtcccggcg tcgaactgct cgtcgaccat cgccttgagc
     1741 gcgtcccgga tcgcggtgtc gaccttcgcc ggatcggact cgccgaggtt cgtagtcagc
     1801 ttgccgatga aatcctggta ggcggcgccg accttggcct ccctcgtcgt ctggtcagtc
     1861 ccggcgccgg tctcttccgt cgcggagggt gtcgcgtcct gggcgacaac ctgagtcacg
     1921 ccgatgccgg ccgcgatacc gagcccggca atgccggcga tcacgttgcg gcgaccgatc
     1981 gggcgatgcc agtgcgactg cccgggctca cgaatcgcgt gctcgtcggt tccatcaaac
     2041 ggtgcgctca tgcggttctc ctggttgcgt tcggtcagct cgaccatcag tcattggccg
     2101 tacggtagcg aaacccggat atggtccgca tatggcccga ccatatgaaa accacagctg
     2161 gattagatga gaatgaaccg ggcgggagtg acatccgggg attgtggccg tgcggatctt
     2221 gttggtcgaa gatgacgagg cgttggcgga tgtaattgcc cgtggcctcc ggcaggccgg
     2281 gctcgccgtc gacatcgcgc cggatggcga atcggcgatc gagagcgcat cgatcaatga
     2341 atacgacgtg gtcgtccttg atcgccaatt gccgggcatc cacggcgacg acgtctgccg
     2401 cgccctgctc cggcgcgatg cgccaccccg gatcctgatg ctgaccgccg ccgggaacct
     2461 tgacgatcgg gtgcgcggac tcgagatcgg ggcagacgac tacctgccca agcccttcgc
     2521 catggcggag ctgatcgccc ggatccaggc cctcggccgc cgtccgagcg cggtccgcct
     2581 gccggtcatc gagatcggcg acctccagat cgactccaat cgccgggcag cccggcgcgg
     2641 ccaccacgac cttgccctga cccgcaagga attcggcgtg ttggagatcc tggcggctga
     2701 gccgggccgc gtcgtcagct ctgaggagct gctcgagaaa gtctgggacg aggcagccga
     2761 tcccttcacg aatgcagtcc ggatcacgat catgaccctc cgccgcaaac tcggcgaacc
     2821 gccgctgatc gagaccgtcg tcggggtcgg ttaccggctt tcagaaccgg catgacaccg
     2881 tttcggcctt ccgtccggtt gaagctgacc ctgctctacg gcgcgctctt cctgattgcc
     2941 ggcctcttcc tgatctcggt gacgtacggt ctggtccggc gcgagctggc gccgaacgcc
     3001 caacggccga tcagccgcgt cgtgcaggac cggccgggca accaccagga cggcccggat
     3061 gatcgcgtcg ccggcgcgcg ccgggaagag cgccgcttcg ctctgcggca gctctggcgg
     3121 cagtcggtgt ttgcccttgc gatcgcgacg gtcggcgcgc tcgggctcgg ctggctcttc
     3181 gccggccagg tgctgcaccc catccggcga atcacggcgc atgcccggtc gtcatcagcg
     3241 gcaacgctcg atgagcgaat ccggcttgat gggccggagg acgagctgaa ggagctggcc
     3301 gacaccttcg attcgatgct ggaccggctc caggccgcgt tccgggcgca gcagggcttc
     3361 gccgcgcagg cctcgcacga gctgcggacg ccgctcgcga tcatccgggc cgaagccgag
     3421 ctggctcgcg atgccccgga cgcctcggag caagaacggc agagcgccga tgcgatcctc
     3481 acggccgccg gccggagtga gcgactggtc gacggtctgc tcgcgctaac ccgcagcgag
     3541 agcacgatgc tccagacggc tccggtcgac ctggccgatc tggccggcga tgtggtcggc
     3601 gaatacgtgc gggaggcgga tcaagccggc gtccgggtag atctcgcgct ggacgcggcc
     3661 acggttcagg gcgatccggc tctgttgcgg cagatgatcg gcaacctcgt ccagaacgcg
     3721 atccgctaca accggccgga cggctgggtc cgggtctccg tcgcgccgac ggatcgcgct
     3781 ggtcgtctcg tggttgagaa cagcggtcaa ctggtcggcc gggaggagat cgaccggata
     3841 ttcgagccgt tcgcgcgcgg cgagtgggcc cggcgcaacc ggacgggatt cggcctcggc
     3901 ctcgccatcg tccgcacggt cgccatcgcc catcacggca cgatccaggc cctcgccggc
     3961 cccgacggcg gcctgaggat cactatcgac atcctcttgc cactccagca ataaccgtct
     4021 tgcgccgccc caaggtaatg gcgcgcaacc cgaaacgcag tgcggggaag ttctgccccc
     4081 gaccgaacgg ggagaagcca gatgggggaa gcgccgctgt gcccgtacgc cacgatggtt
     4141 cctgtttgag gggacggtc
//
LOCUS       DASSGX010000355         2561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_170672, whole genome shotgun sequence.
ACCESSION   DASSGX010000355 DASSGX010000000
VERSION     DASSGX010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170672"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="VFI12_06285"
     CDS             <1..828
                     /locus_tag="VFI12_06285"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HET7056051.1"
                     /translation="AKARRRGVVASHGNATPAAHPSFGEASCSQPGVSTPGNAGHDDP
                     RKTENGKPPKTRDERRETKLETRNEKLETHSGVGIGADGKPRCSWCLGDALYEAYHDE
                     EWGRPLRDDALLFGLLMLEGFQAGLSWRTILHKRENFHEAFHGWDPERIAAYTPDDIA
                     RLLANPGIVRNRLKCEGAVRNATAWLQVREELGSFSDHLWSFVGGETITRSRPTDWTE
                     LPATTPESDAMSKDLRKRGFTFVGSTICYAFMQAAGMVDDHMEGCYLVKGGEVASSR"
     gene            complement(882..1130)
                     /locus_tag="VFI12_06290"
     CDS             complement(882..1130)
                     /locus_tag="VFI12_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056052.1"
                     /translation="MDRSAAGIPDDPICAGCGVALNEPFGWCSNCSAAFCDACAATHF
                     CLPTCQAAGCIAGLCVRRVSDGHLSLEWRKPGTVASEQ"
     gene            1532..>2561
                     /locus_tag="VFI12_06295"
     CDS             1532..>2561
                     /locus_tag="VFI12_06295"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HET7056053.1"
                     /translation="MSDPVRQFRVATAVQMGNGCSANLLASIRAAGGRRVTVVTDPGV
                     RGTGMVERIIEPVQAAGIAVDIDDDVKPNPRDVDCDAGAARAKEFGTDLVVAIGGGSP
                     IDQAKAIAMLVTNGGVATDWQMKRPFEVDPLPLIAIPTTAGTGSEVTRVSVITDTGRM
                     FKMTMGDPRMAPIAAYIDPALTVSVPKGTTAATGMDALTHAIEAYTCTVHNPVSDGLA
                     LRAIRMIAKHLVPAVDDGGNLAAREGMMYASTIAGMAFGNADVAAVHCISEAIGGLYD
                     TPHGIANSVFLPWVFDFNKGADPRRHADVAEALGIARDGRTDDAVAAEGARLLEDLSE
                     RIGIPRFAD"
BASE COUNT          475 a          849 c          845 g          392 t
ORIGIN      
        1 gcgaaggcgc gaaggcgcgg agttgtagcg tcgcatggca atgcgactcc cgccgcgcat
       61 cccagcttcg gagaggcttc gtgttctcag cccggggttt caaccccggg taacgcgggc
      121 catgatgacc cgcggaaaac ggaaaacgga aaaccaccaa agaccaggga cgaaagacga
      181 gagaccaaac tggaaacgag aaacgagaaa ctggaaactc acagcggagt tggcatcggc
      241 gcggatggca agccgcgctg ttcatggtgc ctgggcgatg cgctgtatga ggcgtaccac
      301 gatgaggagt ggggccggcc actgcgggac gatgcgctgc tcttcggctt gctgatgctc
      361 gaggggttcc aggccgggct ctcgtggcga acgatcctgc acaagcggga gaacttccac
      421 gaggccttcc acggctggga cccggagcgg atcgccgcct acacaccgga cgacatcgcg
      481 cggctgctgg ccaaccccgg catcgtccgc aatcggctga aatgcgaggg cgcggtccgc
      541 aacgcgacgg cctggttgca ggtgcgcgag gagctcggct ccttcagcga ccacctctgg
      601 tccttcgtcg gcggggagac gattacccgg tcccggccaa ccgactggac cgagctgccg
      661 gccaccacgc ccgaatcgga cgccatgtcg aaggacctcc gcaagcgcgg cttcaccttc
      721 gtcggcagca ccatctgcta cgccttcatg caagcagccg gcatggttga tgaccacatg
      781 gaagggtgct acttggttaa gggcggggaa gtagccagta gccggtagtc agtagccagc
      841 aagcgtacgt tcaaacgccg gatcaaaaaa ctggctaccg gctactgttc actggctact
      901 gtccccggtt tgcgccattc cagggagagg tggccgtcgc tgacgcggcg cacgcagaga
      961 ccggcgatgc agccggccgc ctggcacgtc ggcaggcaga agtgggttgc ggcgcaggca
     1021 tcgcagaagg cggctgagca attcgagcac cagccgaacg gctcgttgag cgctacgccg
     1081 cacccggcgc agatcggatc atccggaatc ccggccgcgg atcggtccat gcggtgatga
     1141 taggcgagga ggcgagcagc cggaaacggc tgtaggagca gatggcatct gcccgccgtg
     1201 tcctaactgg cccggtcaca tgcgggggag cgcgcccaat accgcccggg cgtaaacacc
     1261 accgcccggg cgtaaacacc cgggctgaat ccaaaaagcc cgctaaagca ggctgagcac
     1321 gcatcttccc aaccgacttc aggcgggttc aggtttcagc cccggtcttc aggccggggc
     1381 ctcgggcagg tgcggcgggg tgtcgcatgg caatgtgacg cgacccgtat tccctccacc
     1441 ctcaactctc caccctcaac cccggctgct cgcctgctcg cctccaaact gctcgcctcc
     1501 catacaatgc ccgtaggttc aggaggcgtg catgtccgat cccgttcgcc aattccgcgt
     1561 tgcgaccgct gtccagatgg gcaacggttg ctcggccaat ctgctcgcat ccattcgcgc
     1621 cgccggaggc cgccgcgtga ccgtcgtcac cgatccaggt gtgcgcggga ccggcatggt
     1681 cgagcggatc atcgaaccgg ttcaggcggc cgggattgcg gtcgacatcg acgacgacgt
     1741 caagccgaat ccgcgcgatg tcgattgcga cgccggcgcg gcgcgggcga aggaattcgg
     1801 gacagacctc gtcgtcgcga tcggcggcgg cagcccgatc gaccaggcca aggcgatcgc
     1861 gatgctggtt accaacggcg gcgtggccac cgactggcag atgaagcggc cgttcgaggt
     1921 cgatccgctg cccctgatcg cgatcccgac caccgccggc accggcagcg aggtgacccg
     1981 ggtctcggtc atcaccgaca cgggccgcat gttcaagatg acgatgggtg acccgcggat
     2041 ggctccgatc gccgcctata tcgacccggc cctgaccgtc tccgtcccca agggcacgac
     2101 cgccgcgacc gggatggatg cgctgaccca cgcgatcgag gcctacacct gcacggtcca
     2161 caacccggtc tcggacggcc tggcgctgcg ggcgatccgg atgatcgcga agcacctcgt
     2221 cccggcggtc gatgacggcg gcaacctggc cgcacgcgag gggatgatgt acgccagcac
     2281 gatcgccgga atggccttcg gcaatgccga tgtggcggcc gtccactgca tctctgaggc
     2341 aatcggcggg ctctatgaca ccccgcacgg catcgccaat tccgtcttcc tgccgtgggt
     2401 cttcgatttc aacaagggag ccgacccccg tcgtcatgcc gacgttgccg aggcactcgg
     2461 catcgcgcgg gatggccgga ccgacgacgc ggttgcggcc gagggtgcgc ggctgctcga
     2521 ggatctctcc gagcggatcg gaatcccgcg ctttgccgac c
//
LOCUS       DASSGX010000356         2809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_390804, whole genome shotgun sequence.
ACCESSION   DASSGX010000356 DASSGX010000000
VERSION     DASSGX010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390804"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="VFI12_06300"
     CDS             <1..1107
                     /locus_tag="VFI12_06300"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET7056054.1"
                     /translation="FVRPMASIDPREHNQSVLRPFGVWAVLAPFNFPFALSAGMSSGA
                     LVAGNTVVFKPASATPLAGYELARCYVDGGVPAGVFNFITGAGEVVGDRLALHEDVDG
                     VIFTGSKDVGLELFKRFSTDFPKPCITEMGGKNPTIVTANADIDKAVDGVARSAFGFS
                     GQKCSACSRVYVEQPVYDEFMTKLTDRVKTLKVGNPEERDVYVGPVIDDKAVARFEKA
                     VAEAKGGTVRAGGSRLTEGEYSKGTFVEPTVVDGLPADHELFKRELFLPFVVVAPVDS
                     LDEAITLSNNTEYGLTAGIFSENEGEIDQFFNGIEAGVTYANRRGGATTGAWPGCQSF
                     CGWKASGSTGKGGLGPYYVQQFLREQSRTRVVEE"
     gene            1144..2196
                     /locus_tag="VFI12_06305"
     CDS             1144..2196
                     /locus_tag="VFI12_06305"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HET7056055.1"
                     /translation="MPSPAHVDLEYMVQATAELCRIPSPTGYTQLVSDRLIEMIGELG
                     LTATPTVKGSLIVTLPGPTTGDGRMLSAHVDTLGAMVKQIKPNGRLKLTNIGGFDWST
                     VEGEFCLVHRANGDPITGTIVTTKSSYHVFAQALVDLKRDAESIEVRLDEIVSSADDV
                     RGMGIAVGDFISFDPKTTVTGNGFIKSRHLDDKACAAVCLGVAKAVVDRSLVLDRPAH
                     LYFSVFEEVGHGTSAGIPEGVTDVIAVDMAAVGEGQTSDERAVTICTKDSSGPYHYGL
                     TRQLIDLAAREGIDYRTDIYPYYGSDVSQALRAGYDIRGALVGPGVDASHAYERLHRD
                     SLEATATLLLAWLCEP"
     gene            complement(2193..>2809)
                     /locus_tag="VFI12_06310"
     CDS             complement(2193..>2809)
                     /locus_tag="VFI12_06310"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET7056056.1"
                     /translation="IAAGDLGSVVLFRAKRNCSRSWFPTYGDRAHTVYETIIHDIDLA
                     LWFTQSRCRSVYAIQRNISGRTYPDALVATLQFENGTMVTLETNWFVPDHAPQNVLAG
                     DWTGTIDAEFELVGTEQTARYRLLDSGMSIATANQIIHPEVGLWPEVHGSIGGALRLE
                     DEHFISCVRTGQQSTIASLDDAHHGLEIAEAIVRSAESRVEVSL"
BASE COUNT          496 a          869 c          930 g          514 t
ORIGIN      
        1 tttgtgcggc cgatggcgtc gatcgatccg cgcgagcaca accagtcggt actacgcccg
       61 tttggggttt gggctgtgct ggcgccgttc aactttccgt tcgccctttc ggccggcatg
      121 tcctcgggcg cgctcgtcgc cggcaacacg gtcgtcttca agccggcatc ggccacgcca
      181 ctggccggat atgagctggc gcgctgctac gtcgatggcg gagttccggc cggagtcttc
      241 aacttcatca ccggcgcggg cgaggttgtc ggggatcgcc tggcgctaca cgaagatgtc
      301 gacggcgtga tcttcacggg gtcgaaggat gtcgggttgg agctgttcaa gcgattcagc
      361 accgattttc cgaagccgtg catcaccgag atgggcggca agaacccgac aatcgtgacc
      421 gccaatgctg atatcgacaa ggcggtcgac ggcgtggccc gctctgcatt tggcttctcc
      481 ggccagaagt gttcggcttg ctcgcgggtc tacgtcgagc agccggtcta cgacgagttc
      541 atgaccaagc tgaccgatcg cgtgaagacg ctcaaggtcg gcaaccccga ggagcgcgac
      601 gtgtacgtcg gtccggtgat cgatgataag gcggttgcgc ggttcgagaa ggcggtcgcg
      661 gaggcgaagg gcggcaccgt ccgggccgga ggatcgcggt tgactgaggg tgagtacagt
      721 aagggcacct ttgtcgagcc gacggtcgtg gacggcctgc cggccgatca cgagctgttc
      781 aagcgcgagc tcttcctgcc attcgtcgtg gttgcgccgg tcgactcgct tgacgaggcg
      841 atcacgctct ccaacaacac cgagtacggc cttaccgccg gcatcttcag cgaaaacgag
      901 ggcgagatcg accagttctt caacgggatc gaggccggcg tgacctatgc aaatcggcgt
      961 ggtggggcga ccaccggcgc gtggccggga tgccagtcgt tctgtggctg gaaggccagc
     1021 ggcagcaccg gcaagggcgg cctcggcccg tactatgttc agcagttctt gcgggagcaa
     1081 agccggaccc gcgtcgttga ggagtagcgc gcggttccgc gccgcgccga atcaggagca
     1141 tccatgccgt cgcctgccca cgttgatctc gagtacatgg tccaggcgac ggcggagctc
     1201 tgccgcatcc cgagcccgac cggctacacc cagctcgtca gcgaccgcct gatcgagatg
     1261 atcggcgagc tcgggctgac ggcaacgccg acagtcaagg gttcgctgat cgttaccttg
     1321 ccgggaccga cgaccggcga cgggcggatg ctctcagccc atgtcgacac gctcggcgcg
     1381 atggtcaagc aaatcaagcc gaacggccga ctcaagctga ccaatatcgg tggattcgac
     1441 tggtcgacgg tcgagggcga attctgcctc gttcatcgtg ccaacggaga tcccataacc
     1501 gggaccattg tgactacgaa atcttcgtac catgtgttcg cccaagcgct tgtcgacctc
     1561 aagcgtgatg ccgagtcgat cgaggtccgg ctggatgaaa tcgtctcgtc ggcggacgac
     1621 gtccgcggga tgggcattgc ggttggcgac ttcatcagct tcgacccgaa gacgaccgtg
     1681 accgggaatg gtttcatcaa gtctcgccat ctcgacgaca aggcgtgcgc ggcggtatgc
     1741 ctcggcgtgg caaaggccgt ggtcgatcgg tcgctggtgc tcgaccggcc cgcccatctc
     1801 tacttcagcg tcttcgagga ggtcgggcac ggcacctcag ccggaattcc ggagggtgtt
     1861 accgacgtga tcgccgtcga catggcggcg gttggcgaag gtcagacctc tgacgagcgt
     1921 gccgtcacga tctgtaccaa ggacagcagt gggccctatc actacggcct cacccgccaa
     1981 ctcatcgacc tggccgcgcg cgaagggatt gattaccgga ccgacatcta cccgtactat
     2041 ggctcggacg tcagccaggc actgcgagcc ggatacgaca tccggggcgc gctggttggc
     2101 ccgggtgtcg acgcctcgca cgcctacgag cggctgcacc gcgattcgct cgaagccacc
     2161 gcaacgctgc tccttgcctg gctctgcgaa ccctagaggc taacctccac acgtgattcg
     2221 gcgctgcgga cgatcgcctc ggcgatctca aggccgtggt gcgcatcgtc gagcgatgcg
     2281 atcgtggact gttgcccggt ccggacgcat gagatgaaat gctcatcctc gagtcgcagg
     2341 gctccgccaa tcgacccgtg gacttctggc cagagcccga cctcgggatg gatgatctgg
     2401 ttcgcggtcg cgatcgacat gcccgagtca agcaaccggt agcgcgcggt ctgctcggtg
     2461 ccgaccagct cgaactcggc gtcgatcgtg ccagtccagt ccccggccag cacattttgc
     2521 ggggcgtgat ccggcacgaa ccagttcgtc tccagcgtca ccattgtccc gttctcgaac
     2581 tggagcgtcg ccacgagagc gtcagggtag gtccggccgg agatgttgcg ctggatcgcg
     2641 tagaccgaac ggcagcggct ctgggtgaac cagagtgcca ggtcgatgtc gtggatgatc
     2701 gtctcgtaga ccgtgtgggc acggtcgcca tacgtcggga accagctccg ggagcaattg
     2761 cgcttggcac ggaagaggac gacggagccg agatcgccgg cggctatcc
//
LOCUS       DASSGX010000357         3071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_281057, whole genome shotgun sequence.
ACCESSION   DASSGX010000357 DASSGX010000000
VERSION     DASSGX010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281057"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..820)
                     /locus_tag="VFI12_06315"
     CDS             complement(131..820)
                     /locus_tag="VFI12_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HET7056057.1"
                     /translation="MSITLESLRGRVEGVRQLVEFSAERVGRNPAAITIVAVSKTVDR
                     DTMMAAYDLGLRNFGENRVPDARQKLADPMPPDTTLHLIGQLQTNKANHAVDLFDVIE
                     SVDRSSLIAELEKQAAKRGITLPVLLQVNVAREEQKAGAAPEEVGSLVNEITWAKHLS
                     LIGLMTMAPLVADPEDVRPVFRKLREMRDHLQSDAGLKLPVLSMGMTNDYMVAIEEGA
                     TEVRVGRAIFG"
     gene            complement(817..1647)
                     /gene="pgeF"
                     /locus_tag="VFI12_06320"
     CDS             complement(817..1647)
                     /gene="pgeF"
                     /locus_tag="VFI12_06320"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HET7056058.1"
                     /translation="MTVSTRLTSPTSDLARHVERSRLLSAIPWVRHGVTRRVPGLGLA
                     DGNVGYTAPRDEADAWEMRQRWAKAIGVDPSDLVRVRQVHGTVVRIATERDTERGTHP
                     DAGEAPIGDAMITAEPNVALMTLHADCLAMLLVDPERRVVGAIHAGWRSTVQDIAGET
                     VREIVATFGGDPGNILAYVGPSIGAERYEVGDEVIDAWRSHPGSSEAPIGRHNGTWRF
                     DLKAANVHQLRMAGLREQNVEISDVCTASETERWFSHRAQGPGTGRFAAVIAITEAWA
                     "
     gene            complement(1644..>3071)
                     /locus_tag="VFI12_06325"
     CDS             complement(1644..>3071)
                     /locus_tag="VFI12_06325"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HET7056059.1"
                     /translation="TFIWANLIGVTLLFLSIGYWLGGRVIDRHPDARWLYALVAIAGI
                     ALALVIPISRPILRASLDAFDDRNAGAFLGSLAATLLLFAPSLLALGMVAPAAIRLRM
                     SSIEDAGKAAGSLYAISTAGSILGSFLPVIVLLPEIGTHATFAAIAIVMIVMSIIGLV
                     AEGRPIPALATAAVFAVAIGALIIGSQGNLKPPYRGQMLAELESDNNYIQVLQDGDET
                     LLALNDGHAVHSIYNPHSLETGGPWDYFALGPILTGDLVPHDALIVGLAGGTAARQLH
                     AAYPGITVDGFEIDQKIVDVGRTYFGLTDQVANVTVADGRYALETTDKTYDLVCLDAY
                     RQPYVPFHLATVEYFQLVSQHLNPHGVVVVNAGRTDTDFRLVDALSTTLRKVFPTVLV
                     VDVDRFDNSMIYATSDAVSVDQFAQRLRETAGVPLVETVSGWALEHGNIREASATGTV
                     FTDDKAPVEWIIDQIIIDEAVREEK"
BASE COUNT          529 a          967 c         1042 g          533 t
ORIGIN      
        1 gcggagtccg gcccggtctg actcggccag cgcctggtcg agcgcgtcat cggcgcgggt
       61 gagctgatcg ccgaaccagg catcgaccga tttccagagt tgctcgtcca tgtgcgctcc
      121 tcctgattga tcaaccgaag atggcgcgcc ccacccgcac ttccgtcgcg ccctcttcga
      181 ttgcgaccat gtagtcgttg gtcatgccca tggagaggac gggcagcttc agcccggcgt
      241 cggactgcag gtgatcccgc atctcgcgca gcttgcgaaa gaccggccga acgtcctcgg
      301 gatcggcaac cagtggcgcc atggtcataa gcccgattag cgagagatgc ttggcccagg
      361 tgatctcatt gacgagcgag ccgacctcct cgggcgcggc gccggctttt tgctcctcgc
      421 gggcgacgtt gacctggagc agcaccggca gggtgatccc gcgcttggcg gcctgctttt
      481 cgagctcggc gatcaacgat gagcggtcga ccgattcgat gacatcgaac aggtcgactg
      541 catggttcgc cttgttcgtc tggagctggc cgatcaggtg cagcgtggtg tcgggaggca
      601 tcgggtcggc cagcttctgg cgcgcgtcgg ggacccggtt ctcgccgaaa ttgcgcaggc
      661 cgaggtcgta ggcggccatc atcgtatcgc ggtccaccgt cttcgagacc gcgacgatcg
      721 tgatcgcggc aggattgcgg ccgactcgtt ccgccgagaa ttccaccagt tgccgaacgc
      781 cttcgacccg gccgcgcagt gactcaagcg tgatgctcat gcccatgcct ccgtgatcgc
      841 gatcaccgcg gcgaaccggc cggtacctgg accctgggca cggtgcgaga accagcgctc
      901 ggtctccgag gcggtgcaga catccgagat ctcgacgttc tgctcgcgga gaccggccat
      961 gcgaagttga tgaacgttcg cggccttcaa atcgaaccgc cacgtcccgt tgtgccggcc
     1021 gatcggtgct tcggatgaac ctgggtgcga tctccaggcg tcgatcacct catcaccgac
     1081 ctcgtaccgc tcggcgccga tcgacggtcc gacatacgcc aggatgttcc cgggatcgcc
     1141 gccgaaggta gcaacgattt cgcgcaccgt ctcgccggcg atgtcctgga cggtcgatcg
     1201 ccagccggca tggattgcgc cgaccacgcg ccgttccgga tcgacgagga gcatcgccag
     1261 gcagtcggcg tggagcgtca tcaaggcaac gttcggttcg gccgtgatca ttgcgtcgcc
     1321 gatcggggct tcgcctgcgt caggatgcgt cccgcgctcg gtatcccgct cggtggcaat
     1381 ccggaccacc gtgccatgta cctgccggac tcggaccagg tcggatgggt cgacgccaat
     1441 cgccttggcc cagcgctggc gcatctccca ggcgtcggcc tcatcgcgcg gagccgtgta
     1501 tccgacattg ccgtccgcca gaccgagccc gggaacccgc cgggtaacgc cgtggcgtac
     1561 ccaggggatc gcggagagca gtcgcgaccg ctcgacgtgc ctggcaaggt ctgaagtcgg
     1621 cgatgtcagg cgtgtggata cggtcacttt tcctcccgga cggcctcgtc gatgatgatc
     1681 tggtcgatga tccattcgac cggcgccttg tcgtcggtga agaccgtgcc ggtcgcggat
     1741 gcttctcgta tgttgccatg ttccagtgcc cagcccgaaa ccgtctcgac aagcgggacg
     1801 ccggcggtct cgcgcaggcg ttgcgcgaac tggtcgaccg agaccgcgtc gctcgtggcg
     1861 tagatcatgc tgttgtcgaa ccggtccaca tcgacgacca ggacggtcgg gaagaccttg
     1921 cggagtgtgg tcgagagcgc atcgaccagt cggaagtcgg tgtcggtccg ccccgcgttg
     1981 acgacgacca cgccatgcgg gttcaggtgt tgcgagacga gctggaaata ttcgaccgtg
     2041 gcgaggtgaa acggcacgta gggctgccgg tacgcgtcga ggcagaccag gtcatacgtc
     2101 ttgtcggtcg tctcgagtgc gtaccggccg tcggcgaccg tgacattggc cacctggtcg
     2161 gtcaggccga agtatgtgcg gccgacatcg acgatcttct ggtcgatctc gaatccgtcg
     2221 accgtgatgc ccgggtaggc cgcatggagc tggcgcgcgg cggtaccccc ggccaacccg
     2281 acgatgagcg catcgtgcgg taccaggtcg ccggtcagga tcgggccgag ggcaaagtag
     2341 tcccacgggc cccccgtctc gaggctgtgc gggttgtaga tggaatggac ggcatgcccg
     2401 tcattgaggg cgagcagcgt ctcgtcgccg tcctgcagga cctggatgta gttgttgtcg
     2461 gattccagct cggcaagcat ctgcccacgg tacggcggtt tcaaattgcc ctgcgatccg
     2521 atgatgagcg cgccgatggc aacggcgaac accgccgccg tcgcgagggc agggatcggc
     2581 cgcccctcgg ccacaagccc aataatcgac ataacgatca ttactattgc gatcgcggcg
     2641 aaggtggcgt gtgtacctat ctccggcagg agaactatca caggaaggaa cgagccgagg
     2701 atcgagccgg ccgtggagat cgcatagagg ctgccggcgg ccttcccggc atcctcgatg
     2761 gaactcatgc gcagacggat tgccgccggc gcgaccatgc cgagcgcgag cagcgacggc
     2821 gcgaagagga gcagcgtcgc ggcgagcgag ccgaggaagg cgccggcatt gcggtcgtcg
     2881 aaagcgtcga gcgaagcgcg caggatcggc cgactgatcg ggatgacgag cgcgagggca
     2941 atgccggcga tggcgaccag ggcgtagagc cagcgggcat cgggatggcg atcgatgacc
     3001 cggccgccga gccagtagcc gatggacagg aagagcagcg tcacgccaat caggttggcc
     3061 caaatgaagg t
//
LOCUS       DASSGX010000358         2578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1044110, whole genome shotgun sequence.
ACCESSION   DASSGX010000358 DASSGX010000000
VERSION     DASSGX010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1044110"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..1919)
                     /locus_tag="VFI12_06330"
     CDS             complement(57..1919)
                     /locus_tag="VFI12_06330"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HET7056060.1"
                     /translation="MTHPFLDRLSSQRLLADGAMGTLIHDAGVPMGTCFDVLNLTNPD
                     LIGAIHRSYLGAGADIIETNTYGANRLKLAEHDLEEKVREINLRAAKIAREAREVVGR
                     EVFVIGSIGPTRSAMDPLMAEEIREIGPVYTEQIQALLEGGVDAICIETISDIREMVV
                     AVNAVRGASDLPIIAMLAFGEDGTTPGGNAVNEVVATLLALGVDVIGANCSVGPNQML
                     PIVQTMTSLVSTTYRRDIPVSTMPNAGWPARVDGRYVFNSSPDYLGRFACEAANAGAR
                     LIGGCCGTTPEHTRAMRRALDEQADANGHVIHEIKLPAIWEFEEHDQDSPTELREKLG
                     NEFVICVEIDPPKGLNPKKALDGARLLKDAGVTAINVADSPMARVRMSALMMSHLIQH
                     EVGVEAIVHFTTRDRSLMAIQSELLGAHAAGVRNILALTGDPPSLGDYPDSSAVYDID
                     SIGLIKVLSKMNEGMDWSGAPIGHKAAFTIACAVDPTREDLQLEASRLRQKLEAGAHF
                     VMTQPIYDVEVWLRFLEIFGGPIPVPVMIGILPLQSARHAEFLHNEVPGITLTEQALE
                     RMRAAGNHGRAVGVEMARELLLDLKPHAQGVYLMPSFGRYEVAAEVLEGVVTRV"
     gene            2084..>2578
                     /locus_tag="VFI12_06335"
     CDS             2084..>2578
                     /locus_tag="VFI12_06335"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HET7056061.1"
                     /translation="MNQKAELVRNDLVDHHPGTVMAIGGAEDKFRERAILSTFMTFAG
                     MSKARIVIIPTASSIETAGERYKGIFLEFGAESAHIAFVHDRTHANEADWSRVFDGAT
                     GIYITGGNQLRLSTRLGGTLIEQEIKRAWHGGATVAGTSAGASILSSHMVAFGTSGPT
                     PRQRM"
BASE COUNT          452 a          837 c          817 g          472 t
ORIGIN      
        1 tgcgagccga ctgccaattc tagcacaaaa tgccggcagt cgcgtcgttc gagcgcctaa
       61 acccgcgtaa cgacgccttc cagcacttcg gccgcaacct cataacgtcc aaacgacggc
      121 atcaggtaga cgccctgcgc atgcggcttg aggtccagca acagttcccg cgccatctcg
      181 acgcctactg cccggccatg attgccggcc gctcgcatgc gctccagcgc ctgctcggtg
      241 agggtaatgc ccggcacctc gttatgcagg aattcggcgt gacgcgcgct ctgaagcggc
      301 agaatcccga tcatgaccgg gaccgggatc ggtccgccga agatctccag gaagcgcagc
      361 cacacttcga cgtcgtagat cggctgcgtc attacgaagt gcgctccggc ctccagcttc
      421 tgcctgagcc ggctcgcctc gagctgcagg tcctcccggg tcgggtcgac agcgcaggca
      481 atggtgaacg ctgctttgtg accgatcgga gcgccggacc agtccatgcc ctcgttcatc
      541 ttgctgagga ccttgatcag gccgatagag tcaatgtcgt agacggccga actgtccgga
      601 taatcgccga ggctcggcgg gtcgccggtc agcgcgagga tattgcggac tcccgccgcg
      661 tgcgcgccaa gcaactcgct ctggatcgcc atcaggctgc ggtcacgggt cgtgaagtgg
      721 acgatcgcct cgaccccgac ttcgtgctgg atcagatgcg acatcatcag cgcgctcatc
      781 ctgactcgcg ccatgggtga gtcggcaacg ttgatcgccg tcacgccggc gtccttgagc
      841 aagcgcgcgc catccagcgc ctttttggga ttgagccctt tcggcggatc gatctcgaca
      901 caaatcacga actcgttgcc cagcttttct cgaagctcgg ttgggctgtc ctggtcgtgc
      961 tcctcgaact cccagatcgc cgggagcttg atctcgtgga tcacgtggcc gttcgcgtcc
     1021 gcctgctcgt cgagcgcgcg gcgcatcgcg cgggtgtgtt ccggcgtcgt tccgcagcag
     1081 ccgccgatca gacgcgcacc ggcgtttgcg gcttcgcagg cgaagcggcc gaggtagtcc
     1141 ggcgagctgt tgaagacgta gcggccgtcc acccgggccg gccaaccggc gttcggcatg
     1201 gtcgaaacgg gaatgtcgcg gcggtaggtc gtgctgacga gcgaggtcat cgtctggacg
     1261 attgggagca tctggttcgg cccgaccgag cagttcgcgc cgatcacatc gacgccgagc
     1321 gccagcaggg tcgcaacgac ctcattcacc gcgttgccgc cgggggtcgt cccatcctcg
     1381 ccgaaggcga gcatggcgat gatcggcaaa tcggaggcgc cgcggactgc gttgaccgcg
     1441 accaccatct cccggatgtc cgagatcgtc tcgatgcaga tcgcgtcgac cccgccttcc
     1501 agcagcgcct ggatctgctc tgtatagacc ggcccgatct cccggatctc ctccgccatc
     1561 agcgggtcca tcgcggagcg agtcggtcca atcgagccga tcacgaagac ctcacgaccg
     1621 acgacctccc tggcctcccg tgcaatcttc gcggcgcgga ggttaatctc tcgcaccttt
     1681 tcctcgagat cgtgctcggc gagcttgagt cggttcgcgc cataggtgtt ggtctcgatg
     1741 atgtcggctc cggctccgag gtaagagcgg tggatggcgc cgatcaggtc cggatttgtc
     1801 aggttgagca catcgaaaca ggtgcccatt ggcacgccgg cgtcgtggat gagggtgccc
     1861 atggcgccgt ccgcaagcaa cctttgggac gagagtcggt cgaggaatgg gtgagtcaag
     1921 gaagtggctc ctggcgaaaa cccggctggt gtctcatacc agagtgagtg cggcaacggt
     1981 gccggcgtgg tttgcaaagc atacacgttg ctgaaagtac gccgaattga cccccgttcg
     2041 cttggcgcgg tagattcccc caacgaagcg accgtccgcg gcaatgaacc agaaggcaga
     2101 actcgtgcgg aatgacctgg tcgaccatca tcctggcacc gtcatggcga tcggcggcgc
     2161 cgaggacaag ttccgggagc gcgcgatcct ctcgacattc atgacgttcg cggggatgtc
     2221 gaaggcgcgc atcgtcatca tcccgactgc ttcgtcgatc gagacggccg gcgagcggta
     2281 caagggcatc ttcctcgaat tcggcgcgga atccgcccac atcgcgttcg tccatgaccg
     2341 cacacatgcg aacgaggcgg actggagccg ggtgttcgac ggtgcgaccg gcatttacat
     2401 caccggaggc aatcagcttc gcctttccac tcgcctcggc ggcacgctga tcgagcagga
     2461 gatcaagcgg gcctggcacg gcggcgcaac cgttgccggt acaagtgccg gcgcctcgat
     2521 cctcagctcg cacatggtcg cgttcggcac gagcgggccc actccccggc agcggatg
//
LOCUS       DASSGX010000359         4951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1261123, whole genome shotgun sequence.
ACCESSION   DASSGX010000359 DASSGX010000000
VERSION     DASSGX010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4951
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261123"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="VFI12_06340"
     CDS             <1..605
                     /locus_tag="VFI12_06340"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7056062.1"
                     /translation="LSDRIGTRWILFGGLLTQVVGIFYIIRQSTLGATPWTLLPALVL
                     TGLGMGFTFAPMTAAVMRDVPPRIAGSASGILNTMRNIGQVLGIAVLGSVLQNRVSAH
                     VGNRLEPVVADPGTRGQIVEYAQQSQFERIAALAPAGQINAVFDAVRHGFTDSIHNTF
                     FVGALICLIAAGVAWFLRDPVQARAEARDETASPAAAVAD"
     gene            875..1948
                     /locus_tag="VFI12_06345"
     CDS             875..1948
                     /locus_tag="VFI12_06345"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HET7056063.1"
                     /translation="MGSIGIPGVPLMGTADRRSLLKRAAALGLAVPAAAGLGGTDVRA
                     QDTGGHDHNATPVPGGIPQGGTGEATPIPGEISPFQRYDPYLKPAEAGPHDIVIEARD
                     QTLYVAKDVAYAAWTFDGTVPGRVFRVTEGDTVNFTLKNSAQMAHSMDFHSAKTPPNV
                     NYKTIPPGQEFSFTFPATTAGAFMYHCGTPPVLMHIGAGMYGTMIVDPKEGWSPAQEL
                     VFVQSEYYLIDSGDGSGVKLPDFKKMMTLNFMDYVVFNGHASQYVDEPIDVKVGELIR
                     IFVMNAGPNVWSSFHVVGAIFDKAYINANPHNELRGLQSITIGPGDGACVEFTVEEPG
                     HYVAVNHAFGHATHGAVAVLNAT"
     gene            1978..2343
                     /locus_tag="VFI12_06350"
     CDS             1978..2343
                     /locus_tag="VFI12_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7056064.1"
                     /translation="MTGPMTDTYISIAEQLARIPPPEAGKRSLILHKSDQLRVILQRM
                     TAGAELREHSAPGEITLQGLSGRFTVSVGNSTYALTDDSLIMLEEGIRHTVVCDEDGA
                     FLLTIAWPHGPDAVSSGEA"
     gene            2350..2817
                     /locus_tag="VFI12_06355"
     CDS             2350..2817
                     /locus_tag="VFI12_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056065.1"
                     /translation="MDRLTSIMLRTALIWLLVGFVIGGLMLTDRRVPGDWRLWLAPGH
                     GHMLFVGWFLQFAIGVAYWLLPRKRSPAQPIGYAERPAYVAVVALNIGLLLRVSAEAA
                     ERSGHASDLTLGILAIAAVLQILAAGIFVVQLWPRVAARPIRSGTSSTKGAPS"
     gene            2814..3035
                     /locus_tag="VFI12_06360"
     CDS             2814..3035
                     /locus_tag="VFI12_06360"
                     /inference="COORDINATES: protein motif:HMM:NF016303.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF542 domain-containing protein"
                     /protein_id="HET7056066.1"
                     /translation="MTETIKTTITDTLIRDLVERYPDTMRVLSPLGIDLCCGGGHPLG
                     EALDLHEIDRATVLPQIEQIVAAQTEQKG"
     gene            3041..4315
                     /locus_tag="VFI12_06365"
     CDS             3041..4315
                     /locus_tag="VFI12_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056067.1"
                     /translation="MGRHLVPATILLAMLAFAVAAFTAGLVPYRGWWWNPAVRLAVVG
                     GITPMIYAVNIRIVPVFSRRNWRSEWMLRAQILLTIAGAWLLFAGGTQRERDVVLMGH
                     VLALAGGVLFLTNTFLLFRQEPLKNRPAPPLPYPEHRAIDKIATRFTSLSGIFLLIGL
                     GIGLYTHIWPPTSGRWDLVWAHTMLVGFFLSMVSGVCYHVLARWTGHRWQSVRLIQIH
                     LYVTLIALPLMLIALATNRETLFSYAGPIQGIALWLFLWNIAPMVWRMTGPTRPALVL
                     AITSLLVGVFLGAMFAGHPVVGVHYRIVHADLNLFGWTGLLISGVAYYLVPRFFGRPL
                     RWPRLAAIQVWLMAAGIVAAALSWTWRIEKGGAEDYVTVTQGVVALSFVLLGVIVSGT
                     LAFGKSGATTISAVPMAKQFKRPQPPLQPSGR"
     gene            4441..>4951
                     /locus_tag="VFI12_06370"
     CDS             4441..>4951
                     /locus_tag="VFI12_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056068.1"
                     /translation="MTENQPESTTTVVTTTEQPAEPKRGWGPFGKNDKPFDNEIERIT
                     IRSYPKIIFLYPSVVFAFICAIFVQADWGSRDNWGLAFMVVLTFNLFVLAFDFPRTAS
                     LTLLFSVIAIVLAAILLNERAEFLPALADQTHKLKPSANATFYWMYAFILTIMLGVVW
                     VIHRFIDYFE"
BASE COUNT          828 a         1722 c         1490 g          911 t
ORIGIN      
        1 ggctgagcga ccggatcggc acccgctgga tcctcttcgg cgggttgctg acgcaggtgg
       61 tcggcatctt ctacatcatt cgccagtcga ccctcggggc gacgccgtgg acccttctgc
      121 cggcactcgt cctgaccgga ctggggatgg gcttcacctt cgcgccgatg accgccgccg
      181 tcatgcgaga cgtgccgccg agaatcgccg gcagcgcctc cggcatcctg aacacgatgc
      241 gcaacattgg ccaggtcctc ggcatcgcgg tgctcggctc cgtgctccag aaccgggtca
      301 gcgctcatgt cggcaaccgc ctcgagccgg tcgtcgcgga ccccggcacc cgtggacaga
      361 tcgtcgaata cgcgcagcaa agccagttcg agcggatcgc cgccctcgcg ccggccggtc
      421 agatcaacgc ggtcttcgac gcggtccgcc atggcttcac cgacagcatc cacaacacct
      481 tctttgtcgg cgcactcatc tgccttatcg ccgcgggcgt tgcctggttc ctgcgtgacc
      541 cggtccaggc ccgagccgag gccagggacg aaaccgcctc gccagcggcc gccgtcgccg
      601 actaggaagc cggacaacac atcgcgtcgc gacgaattga cacgattttg taatgctgat
      661 cgcgcggatc agacattttc gtatgccttt tcgcgtaccc tgatcgtcag gatgcggtgg
      721 ggcgcttagc ctacctgcca gagcttcttc ggagtgcccc gcccgcatct gtcctacatg
      781 cgcatgggga gcgcgtccgc ggactgcaat tgggctgtct gtgtcgttcc cgaagaaggc
      841 agggcgaacg gtccctgtca ggaggctaag aaacatgggt tcgattggga ttcctggcgt
      901 cccgctgatg gggacggctg atcgacgctc actcttgaaa cgggcggcgg cgctcggtct
      961 cgcggtcccg gcagcggccg gcctcggcgg aacagacgtt cgcgcccagg acaccggcgg
     1021 gcacgaccac aacgcgaccc cggtccccgg cggaattccg cagggtggca ccggtgaagc
     1081 aaccccgatt cccggtgaga tatcgccgtt ccagcgatac gatccgtatc tcaagccggc
     1141 agaagccggt ccgcatgaca tcgtgatcga ggcgagagac caaactctct acgtcgccaa
     1201 ggacgttgcc tacgcggcgt ggaccttcga tggcacagtt ccgggccgcg tcttccgcgt
     1261 gaccgagggc gataccgtca acttcaccct caagaacagt gcgcaaatgg cacactcgat
     1321 ggactttcac tccgccaaga cgccgccgaa cgtgaactac aagacgatcc cgcccggcca
     1381 ggagttctcc ttcacattcc cggccaccac ggccggcgcc ttcatgtacc actgcggcac
     1441 gcccccggtc ctgatgcaca tcggcgccgg catgtacggc acgatgatcg tcgaccccaa
     1501 ggaaggctgg tcgccggccc aggagctcgt cttcgtccag agcgagtact acctgatcga
     1561 cagcggggac ggcagtggcg tcaagctgcc cgacttcaag aagatgatga ccctgaactt
     1621 catggattac gtcgtcttta acgggcatgc ctcccagtac gtcgacgagc cgatcgacgt
     1681 caaggtcggt gagttgatcc gcatcttcgt catgaatgcc ggaccgaacg tctggtccag
     1741 cttccacgtc gtcggcgcga tcttcgacaa ggcctacatc aacgccaacc cgcacaatga
     1801 gctgcgcggg ctccagtcga tcacgatcgg ccctggcgat ggcgcttgtg tcgagttcac
     1861 cgtcgaggag ccgggccact acgtcgccgt caaccacgcc ttcggccacg ccacccacgg
     1921 ggcggtggcg gtcctgaatg cgacgtaggc ccaaccagca ggatcaggag gaacagcgtg
     1981 acaggcccga tgaccgacac gtatatctcg atcgcggaac agctcgcccg gataccgcca
     2041 cccgaggcgg gaaagcgatc cctgatcctg cacaaatccg atcaactgcg cgtcatcctg
     2101 cagcgcatga ccgccggcgc cgaattgcgc gagcactcgg cgcccggcga gattacgtta
     2161 caggggctga gcggccgatt tacggtttcg gtcgggaata gcacctacgc tctgaccgat
     2221 gacagcctga tcatgctcga agagggtatc cgccacacgg tcgtctgcga cgaagatggc
     2281 gccttcctgc tcacaatcgc ctggccccac ggacccgatg cggtctcgtc cggagaggcg
     2341 tagccatcgg tggatcgcct cacgagcatc atgctccgga ctgcgctgat ctggctgctc
     2401 gtcggcttcg tgatcggcgg cctgatgctg accgaccggc gggtacccgg cgattggcgg
     2461 ctctggctcg cgccgggaca cgggcacatg ctctttgtcg gctggttcct gcaattcgcg
     2521 atcggtgtcg cctattggct cctcccccga aagcgatcgc cggcccagcc catcgggtac
     2581 gccgagcggc cggcgtacgt cgccgtcgtg gcgctcaaca ttggcctgct gctgcgcgtc
     2641 tcggctgagg cagcggagcg ctccggtcac gcgagcgacc tgacgctcgg aatactcgcg
     2701 atcgccgccg tgctgcaaat cctggcggcg gggatcttcg tcgtgcaact ctggccgcgc
     2761 gttgcggcca ggccaattcg atccggtacg tcgtccacaa aaggagctcc atcatgaccg
     2821 agacgatcaa aaccaccatc accgacaccc tgatccgcga cctcgtcgag cgataccccg
     2881 acaccatgcg cgtcctctcg ccgctgggca tcgatctctg ctgcggcggt ggtcacccgc
     2941 tcggcgaggc gctcgacctg catgagatcg accgcgccac cgtcctgccg cagatcgagc
     3001 agatcgttgc cgcgcagacg gagcagaagg gctaacagac gtggggcggc acctcgttcc
     3061 ggcaacgatc ctgctggcga tgctggcatt cgcggtcgcc gccttcacgg ccggcctggt
     3121 tccctatcgt ggctggtggt ggaacccggc cgtccgcctg gcagtcgtcg gcggcatcac
     3181 gccgatgatc tacgcggtca acatccggat cgtgcccgtc ttctcccggc gcaactggcg
     3241 ctcagagtgg atgctgcggg cacagattct gttgaccatc gccggcgcat ggctgctctt
     3301 cgccggcggc acccagcggg agcgggatgt ggtcctcatg ggccatgtcc tggctctcgc
     3361 cggcggcgtc ctcttcctca ccaatacctt cctgctcttc cgacaggagc cgctcaagaa
     3421 ccgcccggcg ccgccgctcc cctaccccga gcatcgcgcg atcgacaaga tcgcgacccg
     3481 cttcaccagc ctctccggca tctttctcct gatcggcctc ggcatcggcc tctacaccca
     3541 catctggcct ccgacgtcag gccgctggga cctcgtctgg gcgcacacga tgctggtcgg
     3601 cttcttcctg tcgatggtct cgggcgtctg ctaccacgtg ctggcccgct ggaccggcca
     3661 tcgctggcaa tcggtccgcc tgatccagat ccacctgtat gtcacgctca ttgcgctgcc
     3721 gctgatgttg atcgccctcg ccacgaatcg tgagacgctt ttcagctatg ccggaccgat
     3781 ccagggaatc gccctctggc tgttcctctg gaacattgcg ccgatggtct ggcggatgac
     3841 cggcccgacc cggccggccc tggtcctggc gatcacatcg ctgctcgtcg gcgtcttcct
     3901 gggggcgatg ttcgccggcc acccggtcgt cggggtccac taccggatcg tccacgccga
     3961 cctcaatctg ttcggctgga ccggcttgct gatctcgggc gtcgcctact acctggtgcc
     4021 gcgcttcttc ggccggccgt tgcgctggcc gcgactggcc gcgatccagg tctggctgat
     4081 ggcggccggc atcgtggcgg ccgcactctc ctggacctgg aggatcgaga agggcggagc
     4141 cgaagactac gtcacggtca cccagggcgt ggtcgcgctc agtttcgtgc tgctcggcgt
     4201 gatcgtctcc ggcaccctcg ccttcggcaa gagcggcgcc accaccatct ccgccgtgcc
     4261 catggccaag caattcaagc gcccccaacc cccgctccaa cccagcggcc gctaatttcc
     4321 ggaatcctga cgtttggacc agcgtttcga tcccgcccga cgtgctataa gctatgccgt
     4381 gtacgtacga ccgtgatgag cgcgatcagc cgggccgggg tggccaggag cactgcggga
     4441 atgacagaga atcaacccga atcaaccacg acggtggtca ccaccacaga gcaaccagcc
     4501 gaaccgaagc ggggctgggg gccgttcggc aagaacgata agccgttcga caacgagatc
     4561 gagcggatca ccattcgctc ctacccgaag atcatcttcc tctatccatc ggtggtcttc
     4621 gcgttcatct gcgccatctt cgtccaggcc gactggggca gccgcgacaa ctggggcctg
     4681 gccttcatgg ttgtgctgac gttcaacctg ttcgtgctgg ccttcgactt cccgcgcacg
     4741 gcctcgctga ccctgctctt ctcggtgatc gccatcgtgc tggcggccat cctgctcaat
     4801 gagcgggccg agttcctgcc ggcgctggcc gaccagaccc acaagctcaa accctctgcc
     4861 aacgcgacct tctactggat gtacgccttc atcctgacga tcatgctcgg ggtcgtctgg
     4921 gtcatccacc gtttcatcga ctacttcgaa g
//
LOCUS       DASSGX010000360         2512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1481754, whole genome shotgun sequence.
ACCESSION   DASSGX010000360 DASSGX010000000
VERSION     DASSGX010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1481754"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..177)
                     /locus_tag="VFI12_06375"
     CDS             complement(<1..177)
                     /locus_tag="VFI12_06375"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7056069.1"
                     /translation="MTAVATHRHDVIVVGAGVAGCSAAYRLAQAGRKVLVLEKRGIAS
                     GASGRNGGMTGVGSA"
     gene            complement(266..616)
                     /locus_tag="VFI12_06380"
     CDS             complement(266..616)
                     /locus_tag="VFI12_06380"
                     /inference="COORDINATES: protein motif:HMM:NF014677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HET7056070.1"
                     /translation="MDDQLDGLGIRVRAYFGERDHHHGKPLWSALLEFLRTNGSAGAT
                     VTRGIAGYGAHSKIHAASIVDLSSDLPLILEWVDSDERVAALLPGLEEMLQGGLITTD
                     PVTVIRYQPHADRA"
     gene            739..1131
                     /gene="crcB"
                     /locus_tag="VFI12_06385"
     CDS             739..1131
                     /gene="crcB"
                     /locus_tag="VFI12_06385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HET7056071.1"
                     /translation="MSAFLLVSAGGFVGANVRYGISLWAARRFGAGFPVGTLIANLGG
                     SFLMGFALGLLAGWFDDDRGARLLLATGFLGAETTFSTWTWETMALIREGQIRDALRN
                     LLGSIGLGVIAATLGLVLAWLLTDGLTG"
     gene            1128..1538
                     /gene="crcB"
                     /locus_tag="VFI12_06390"
     CDS             1128..1538
                     /gene="crcB"
                     /locus_tag="VFI12_06390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HET7056072.1"
                     /translation="MSDLAHTGWRATIPTVLAVGIGGIVGANLRWQVGEWAADRWATP
                     FPWGTLVINLTGSFVLGLYLTLVTERFAGRPLTRLFFATGLLGAYTTFSTFAYETVRL
                     LQDGHLVTAATYVAASLVFGLVASIAGIAVARMR"
     gene            1595..>2512
                     /locus_tag="VFI12_06395"
     CDS             1595..>2512
                     /locus_tag="VFI12_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056073.1"
                     /translation="MRNLLGRLNARSVQDLSRIADAWQTPLAGRDRLAQISQLYRTMT
                     RPSMVRAQWDGLDPDQQTVVRALLDAADTGRTIDELADELGAAPDLVRVMCVDLYDQG
                     VIAYEGNASTLPVGEQPRLSVPIELASAIRAAIREIEAGDVSDRPFAELIQTRDERDL
                     FDAASYWGIEVIPGVTTRAQMVDSLTKAAAVGATRHAQVDKLGQEVRALWEKLRAVPP
                     GTPVPVEDLIGAGSERAMYGRRNAINELEDRLLAWPTVLDGGARALFVPAEVANAEGN
                     TGPDAARPKPVSVVGSEPPYRPPGPLAWDL"
BASE COUNT          377 a          823 c          893 g          419 t
ORIGIN      
        1 ggccgagccg acgccggtca tgccaccgtt gcgcccggac gcgcccgagg cgatcccgcg
       61 cttctcgaga accagtacct tgcggccggc ctgcgccagc cggtaggcgg ccgagcagcc
      121 ggcgacgccg gctccaacga cgatgacatc atgacgatgg gtggcgacag ccgtcacaga
      181 acttcactcc aggactcagc gcgcgacgaa cgcggtgcat gggctcgatt gcgtgtcggc
      241 gcacagtatg cgggatgttc cgggctcagg cgcggtcggc atggggctgg tagcggatga
      301 cggtgaccgg gtcggtcgtg atcaggccgc cctggagcat ctcttcgagc cctggcagca
      361 gcgccgcgac gcgctcgtcc gaatcgaccc actcgaggat cagcggcaga tcggaagaaa
      421 ggtcgacgat cgaggcggca tggattttgc tgtgcgcgcc gtatccggcg atgccccggg
      481 tgacggtcgc cccggctgag ccgttcgtcc gcaggaactc gagcagcgcg ctccagagcg
      541 gtttgccgtg atgatggtcg cgctcgccga agtaggcgcg gacgcgaatt ccgagtccat
      601 caagttgatc gtccatgccg gtctcccgcc agaaatcgcc tgatgacgtc actgtagtcc
      661 ggacgtccag tggtctgcga cactttcgcg catgacgctg aacagagatc gcaccgcgac
      721 cagtctgggc gagggccgat gagcgcgttc ctgctcgtca gcgccggcgg atttgtcggc
      781 gcgaacgtgc gctacggcat ctcgctctgg gccgcgcggc gcttcggcgc cggctttccg
      841 gtcgggacac tgatcgccaa cctcggcggc tccttcctga tggggttcgc gctcggactt
      901 ctggcgggct ggttcgatga cgaccggggg gcgcgcctgc tgctcgcgac cggctttctc
      961 ggtgcggaga cgaccttctc gacatggacc tgggagacga tggcgttgat ccgggaggga
     1021 cagatccggg atgctctccg gaacctcctc ggcagcatcg ggctcggcgt gatcgcggcc
     1081 acgctcggcc tggtgctcgc ctggctgctg acggatggcc tgacggggtg agcgatctgg
     1141 cgcatacggg atggcgggcg acgatcccga ccgtgcttgc cgttggcatc ggcggaatcg
     1201 tcggggccaa cctgcgctgg caggtcggcg agtgggcggc cgatcgatgg gcgacgccct
     1261 tcccgtgggg cacgttggtg atcaacctga ccggcagctt cgtcctgggc ctctacctga
     1321 cgctggtgac ggagcgcttc gccgggcgac cgctgacccg gctcttcttc gcaaccggtc
     1381 tcctcggcgc ctataccacc ttctccacct tcgcctacga gacggtgcgg ctccttcagg
     1441 acgggcatct cgtgactgcg gccacctatg tggccgcgag cctggtgttc gggctggtcg
     1501 caagcatcgc cgggatcgcc gtggcccgga tgcgctaggc cgccactgtc ccggggtggc
     1561 ggtatcatgg ccgttatgta gtgacagggt gagtatgcgc aatctgctcg gtcggctgaa
     1621 cgcccgatcg gttcaggacc tctcccggat agccgacgcc tggcagacgc ccctcgcggg
     1681 ccgggatcgg ctggcccaga tcagccagct ctaccgcacg atgacccgtc cctcgatggt
     1741 gcgcgcccag tgggatggac tcgatcccga ccaacagacc gttgtccggg cgctgcttga
     1801 cgcggctgac acgggccgga cgatcgacga gctggcagac gagctcggcg ccgcgccgga
     1861 tctcgtccgg gtgatgtgcg tcgatctcta cgatcagggc gtgatcgcct acgaaggcaa
     1921 cgcgtcgacg ctgccggtcg gcgagcagcc gcggctgtcc gtgccgatcg agctcgccag
     1981 tgcgatccgg gccgcgatcc gcgagatcga ggccggcgat gtcagtgacc ggcccttcgc
     2041 agaattgatt cagacccggg acgagcgcga cctgttcgat gccgcgagct actgggggat
     2101 cgaggtcatc cccggcgtca cgacccgtgc ccagatggtc gactcgctga ccaaagccgc
     2161 ggccgtcggc gcgactcgcc atgcgcaggt cgacaagctt ggccaggagg tccgcgcgct
     2221 ctgggagaag ctccgggccg tgccgccggg aacgccggta cctgtcgagg acctgatcgg
     2281 cgcgggcagc gaacgggcaa tgtacggccg ccgtaacgcg atcaacgagc tggaggaccg
     2341 gctactggcg tggccaacgg tgctggacgg cggcgcgcgg gcgttgttcg tcccggccga
     2401 ggtggcgaac gccgagggca acactggtcc ggacgcagcc cgtccaaaac cggtaagcgt
     2461 tgtcggatcg gagccgccat atcgcccgcc gggaccgctg gcgtgggatc tg
//
LOCUS       DASSGX010000361         2500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_391455, whole genome shotgun sequence.
ACCESSION   DASSGX010000361 DASSGX010000000
VERSION     DASSGX010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391455"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..1087
                     /gene="modA"
                     /locus_tag="VFI12_06400"
     CDS             200..1087
                     /gene="modA"
                     /locus_tag="VFI12_06400"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7056074.1"
                     /translation="MRRTVSVSAVMILLVTLMAGLMPDLTRAQAQGWSCDVPVAATPG
                     ATPATALTPKARPFPTEGGNLTVFAAASLTDAFTKMADDLKGAHPNLNITFNFAGSQA
                     LVTQIAQGGAQADVLALASPAQMKSAAEAGIPAEGAATFVTNRLAVVVPADNPAGITA
                     YADLAKAGVRLVLANPDVPAGAYARQSLCKAAAAGGPTTDDYAANIVSEEDNVRSVLT
                     KVQLGEADAGIVYVTDAMTAGGKVRTIEIPADQNVVASYPVAAISDNPLAAAFVGYIF
                     SPEGQATLASFGFDPVNTP"
     gene            1126..1905
                     /locus_tag="VFI12_06405"
     CDS             1126..1905
                     /locus_tag="VFI12_06405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7056075.1"
                     /translation="MPWLVGGVLLLFLALPLFALSWRAIAHLDNLHSAGRTTVADALV
                     LSATTSLTSLVLIVGFGTPLAFGIARRSFPGKSLLETLIDLPMVLPPAVAGLALLMAF
                     GRRGVLGTWLDDAGIRIGFTTTAVVFAQLFVSSPFYVRSARAAFSRLDRTVEDAAADL
                     GASPARVLRTVTLPLAAPGLMAGAVLAWARAVGEFGATIMFAGNLPRETQTLPLAIYG
                     RYEAGDLDTALAMALILLASSFLVLVSVRGLGGRLRLSAAD"
     gene            complement(1837..>2500)
                     /locus_tag="VFI12_06410"
     CDS             complement(1837..>2500)
                     /locus_tag="VFI12_06410"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7056076.1"
                     /translation="LIVPRASARQTMAGSIGQATYEPIAEADFTFRPLSPCDFPVMFR
                     WRINPAVAQRWDPAPDTIDELEPEFGPMIDGAEPVNGVIVEHAGTPIAFIQWYRLHDH
                     PDYSGIDLAPAGAAGIDLFIGEDDYRYRGYGAPMLRAFLREVLFAQPDITACAIDPLV
                     ENTSAISAYRRTGFREAGIRYNAYEHGDSLIMLIDRADLIGSREAQAAAEAADADQDQ
                     KG"
BASE COUNT          390 a          826 c          849 g          435 t
ORIGIN      
        1 catcgagatc ggagaagagc gtcggcgagt agagcgattc cggcatcggc gggtcctcca
       61 cgattgcgcc tgtcacccag gagtggacga taccagcaat gggccggacc gggtcctgcg
      121 ttgacagcaa ttgccgactg ggataggctc ggctgcggca catagtcaat cattgagtat
      181 tgagaccggg agtgggcagg tgaggcggac cgtcagcgtt tcagccgtga tgatcctgct
      241 ggtgacgctc atggccgggt tgatgccgga tttgactcgc gcacaggcgc aggggtggtc
      301 gtgcgatgtg cccgtcgccg caacgccggg cgcaaccccg gcaaccgcgt tgacacccaa
      361 ggcgaggcca tttccgactg aaggtggcaa cctgaccgtc ttcgctgcgg cctccctgac
      421 ggacgccttc acgaaaatgg ccgacgatct caagggtgct catccgaacc tcaacatcac
      481 cttcaacttc gccggatcgc aggcgctggt cacgcaaatc gcgcagggcg gtgcgcaggc
      541 cgatgtgctg gcgctggcca gtcccgccca gatgaagagc gccgccgagg ccggcattcc
      601 ggccgagggg gcagcaacgt tcgtgacgaa ccggctggcc gtcgtcgtac cggccgacaa
      661 cccggccggc atcacggcat acgccgatct cgcaaaagct ggcgtgcggc tcgtcctggc
      721 caaccccgat gtcccagccg gcgcctacgc ccggcaatcg ctctgcaagg ccgcggcagc
      781 cggcggaccg acgaccgacg actatgcggc gaatatcgtc tcggaagagg acaacgtgcg
      841 ctcggtcctg accaaggtcc agctcggcga agccgacgcg ggcatcgtgt atgtcaccga
      901 cgccatgacc gccggcggca aggtccgcac gatcgagatt ccggccgatc agaatgtggt
      961 cgccagctac ccggtcgccg cgatcagcga caatccgctt gctgccgcct tcgtcgggta
     1021 tatcttctcg ccggaagggc aggcgacgct ggcctcgttc ggattcgacc cggtgaacac
     1081 gccctgagcg cggaaccaga caacccggcg cattcgcgat cggccgtgcc atggcttgtg
     1141 ggcggcgttc tgctcctgtt cctggcgttg ccgctcttcg cgctctcttg gcgagcgatc
     1201 gcgcacctcg acaacctgca ctcggcgggc cgcacgaccg ttgccgacgc cctggtcctg
     1261 agcgcgacga cctcgctgac cagcctggtc ctgattgtcg gcttcgggac accgctggcg
     1321 ttcgggatcg cccggcgctc cttcccgggc aagtcgctac tggagacgct gatcgacctg
     1381 ccgatggtgt tgccgccggc ggtggcgggt ctggcgttgc tgatggcctt cggacgacgg
     1441 ggcgtgctcg ggacgtggct ggacgatgcc gggatccgga tcggcttcac gaccacggcg
     1501 gtcgtcttcg ctcagctctt cgtctcgtcg ccgttctatg tccgctccgc ccgggccgcg
     1561 ttcagccggc tggatcgaac cgtcgaggat gccgcggccg acctcggggc ttcgccggcg
     1621 cgggtcttgc ggaccgtcac cctgccgttg gcagcgccgg ggctgatggc cggcgcggtg
     1681 ctggcctggg cgcgggcggt cggcgagttc ggcgcgacga tcatgttcgc cggcaacctg
     1741 ccccgcgaga cgcaaacgct gccgctcgcg atttacgggc ggtacgaagc tggcgacctc
     1801 gacacggcgc tggcgatggc gctgatcctg ctcgcctcat cctttttggt cctggtcagc
     1861 gtccgcggcc tcggcggccg cctgcgcctc tcggctgccg attaggtcgg cccggtctat
     1921 cagcatgatc agggaatcgc cgtgctcgta cgcgttgtag cggataccgg cctcgcggaa
     1981 tccggtccgg cggtaggccg agatcgccga cgtgttctcg acgaggggat cgatggcgca
     2041 ggccgtgatg tcgggctgtg cgaagaggac ctcacgcagg aatgcgcgca gcatcggcgc
     2101 gccatacccg cgatatcggt agtcgtcctc gccgataaag aggtcgatgc cggccgcgcc
     2161 cgccggggcg agatcgatgc cggagtagtc agggtgatca tgcagccggt accactggat
     2221 gaacgcgatc ggggtgccgg cgtgttccac gatgacccca ttgaccggct cggcgccgtc
     2281 gatcatcggg ccgaactccg gttccagctc gtcgatcgtg tccggagccg ggtcccaccg
     2341 ctgcgcgacg gcgggattga tccgccagcg gaacatcacc gggaagtcgc acggagataa
     2401 cggccggaag gtgaaatcgg cttccgcaat cggttcgtaa gtcgcctgtc ctatactgcc
     2461 cgccattgtt tgcctcgcac tcgccctggg aacgatgaga
//
LOCUS       DASSGX010000362         7899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_172455, whole genome shotgun sequence.
ACCESSION   DASSGX010000362 DASSGX010000000
VERSION     DASSGX010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7899
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172455"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(206..1363)
                     /locus_tag="VFI12_06415"
     CDS             complement(206..1363)
                     /locus_tag="VFI12_06415"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HET7056077.1"
                     /translation="MRVLVVLMVLVASAIAVAQPARAATCIVAGQSYVVTIRGSRYIT
                     GTVGDDVILGSSGRDVITGNGGNDVVCGGAGNDDMTLGAGNDKVNGGSGRNTIRGGAG
                     DDLLIGGSGDDSITGQGGNDVIAGGDGVGKLVGGNGDDQITGGADADRIYGQTGRDRL
                     DGGAGNDSISAGDDDDQVIGGPGDDKLVAGSGNDVITGGDGNDRLYGQSGADQMAGDD
                     GDDSLSGNGDADILTGGAGVDILSGWSGDDQLFGGDGNDELYGQDGDDLIDGGNDHDY
                     IKGDDGADTEFGGDGNDVMHDGLGDDQVSGGPGNDLIQGWYGDDIYWGAEGDDRINGW
                     DGNDTIYGGIGDDQLSGDAGEDTCDGEDDTAATGGPGDTADATCEVQIGIP"
     gene            1577..1810
                     /locus_tag="VFI12_06420"
     CDS             1577..1810
                     /locus_tag="VFI12_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056078.1"
                     /translation="MTRLFRWTVDLDALTDEAYAFVTDPRPSRNERPVIQELHDINPE
                     TVDVLESMLIDGTEERRDVADYIDAVFGPYAAR"
     gene            complement(1843..2970)
                     /locus_tag="VFI12_06425"
     CDS             complement(1843..2970)
                     /locus_tag="VFI12_06425"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET7056079.1"
                     /translation="MIATPEVVPAADSASLEAIEDAFRAQISPMRLFPGAALAAYQHG
                     ELALDLVGGYADTQSGELVDSSSLFPLFSGTKPFGAVALWQQIERGKLALDDRVAEYW
                     PAFGVNGKEEVLVRHILSHRGGFPTTPPELAMEMWGDAAVVNRAIERMPLAHPPGTAP
                     AYHFLTQHWVIGELIRRLDGRTFDAYLDDEITGPLGLRDTFVGLPAMHDGRVVKLHAT
                     DGTDELSLTALRETRSVPFYRMVIPGASGVSSARDMARFYAAIAAGGSIDGATILQPE
                     TVDRMMEIEIDGDIDLTFDAPVRRGLAFELGGLADPRRHWPGATSTERTFWHGGMGSS
                     VCWADRDTELTMAFLSNGIRRDAAGAIARRDLSDLVRSTHR"
     gene            complement(2984..4552)
                     /locus_tag="VFI12_06430"
     CDS             complement(2984..4552)
                     /locus_tag="VFI12_06430"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-coumarate--CoA ligase family protein"
                     /protein_id="HET7056080.1"
                     /translation="MIIRSPHPDIAIPDIALTPFILRHAERLAAKPALIDGVSGRTMT
                     YQELARAIRGTAAGLAARGFRAGDVFAIYAANCIEYAVAFHAVSWLGGVMTTVNPAYT
                     ADELEHQLKDSGASRLLTTPDLMAKAGPAARGAGITEIYSTGKADGTIPFADVSESVG
                     DPPVAIVDAANDTVALLYSSGTTGLPKGVMLTHRNLVANIEQLAAVRPYGQDDVALCL
                     LPIFHSFGLQVLLNHGLAVGATLVLLPRFEIESFLTAIERYGVTYTALVPPVVLILST
                     HEAVDRYDVTQLRRINCGAAPLSAGTSRDCASRLDCAVEQGYGLTETSPVAHMTIPGR
                     NIEGSVGPLVPNTEAKIVDLVSGESLGPNSPGELWVRGPQITKGYLNRPDATATTIDE
                     DGWLHTGDIACVDEAGSFAIVDRLKELIKYKGYQVAPAELEAVLLTHPAVADAAVIPS
                     PDLDTGEVPKAFVVLRGPAGADELMAFVADRVAPHKKVRRVEFIDCIPKSPTGKILRR
                     VLVEQERVALAVAG"
     gene            complement(4752..7016)
                     /locus_tag="VFI12_06435"
     CDS             complement(4752..7016)
                     /locus_tag="VFI12_06435"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7056081.1"
                     /translation="MVGRQRELAAVRDLLLRPDVPMVTVTGPGGVGKTRLAMHVAGDL
                     AAAFRDDARFVPLAAIADVDLVIPAIAQGLGLSGLGGQSAEVGLRDFLQDRELLLVLD
                     NMEHVIGAAPALSSLLRACPSLTLLVTSRETLRVEGEQEYPLKPLAVPDVDTRATAES
                     VAGTDAVALFVQRARAAQPGFALDEGNAAAVAAICSRLDGLPLAIELAAPRIKMLPPQ
                     ELLNRLATRFNLLSRDARDMPARLRTMRDAVGWSYDLLPLEEQALFRQLSIFGGGFSL
                     EAAEALFAEGSDPAGSILERISSLEEKSLVRRVEDSAAPRFGMLETIRRFGFELLVAY
                     GESDAALNAMSSWCLELFSSGFEHMFGPEQGTWLKIFDAEYDNVRIALGWLLENGEAA
                     RAQLLAVAMGRYWSVRGYFAEGRSWIERALAAGPTNRQSYGHALLVGGWLALQLGDRE
                     SAKRMIDEGIELAQDTADQHWYGQSLTVLGFVLEDMGRVDEAVARHEEALAVYRSYGD
                     AVWPAYVLNSLGLAAYRQGDLERAVPNLRQSLAEFRDAGNSYGESLVLMNLGRVARRR
                     GEYAEAHGLMVDSLALRWEQGDRPGILSCLRGLATLALLTRKHADAARLLGAAEALRG
                     EIAAPEPRDHDRYDCEIDRARSALGEVDFAAAKKAGSETALREIASGILGGNGPNARG
                     AATPGQARRSGLTPREVEVLRLIRDGLSNRDIGERLFVSERTAQTHVQHILDKLDVGT
                     RAEAAADAVERGLI"
     gene            complement(7187..>7899)
                     /locus_tag="VFI12_06440"
     CDS             complement(7187..>7899)
                     /locus_tag="VFI12_06440"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET7056082.1"
                     /translation="AIRDDLRSLPNGDMRLLPTGLELPIRHFAERMIAGSDNTATDHL
                     IGRLGRPRIQAGFADQGHSRPDANRPLLMTREWFAIRMRLRDTQIERYLAADAGKRLR
                     ILANTVDPLAAQLSDAEPWPGPRFLDRIEWFAGGADLVRAIEWLWRRSDGVEGAPVRD
                     ALSLNPGSCWHPDTWRYVGYKGGYETGAMSNSWLLQRQDGRWFAFSAVINDTQREIDA
                     NGLWLLQTAAERLLAATD"
BASE COUNT         1390 a         2704 c         2425 g         1380 t
ORIGIN      
        1 cacgcagctc gatgatgacc ggctcgttcg attcgatcac ttcggtcatc aatgcgccca
       61 gtcgattctt tgcctcagta gctgtgactg tcttggtcat gacttgctcc ttagctattt
      121 cattagccaa tataatagct atcggggacc gagtgagcaa tcactcttgc cagccaacgg
      181 actgccctgt cccgggcttt gcccgtcagg ggattccgat ttggacctcg caggtggcgt
      241 cggcggtgtc gcccgggccg ccggttgccg cggtgtcatc ttcaccgtcg caggtgtctt
      301 cgccggcatc gcccgagagt tggtcgtcgc caatgccgcc atagatggtg tcgttgccgt
      361 cccagccgtt gatgcggtcg tcgccttcgg cgccccagta gatgtcgtcg ccgtaccagc
      421 cctggatcag gtcgttgcca ggcccgccgg agacctgatc gtcgccgagg ccgtcgtgca
      481 tgacgtcgtt gccgtcgccg ccgaactcgg tgtccgcgcc gtcgtcgccc ttgatgtagt
      541 catggtcgtt gccgccgtcg ataaggtcgt cgccgtcctg gccgtagagc tcgtcgttgc
      601 cgtccccgcc aaacaactgg tcatccccgg accagccaga gaggatgtcc acgcccgctc
      661 cgccggtcag gatgtcggcg tccccgtttc cggagaggga gtcgtcgccg tcatcgcccg
      721 ccatctgatc ggcgccgctt tgaccgtaga ggcgatcatt gccatccccg ccggtgatga
      781 cgtcattacc cgatccggcg accagcttgt cgtcgcccgg accgccgata acctggtcat
      841 cgtcatcgcc ggccgagatc gagtcattcc ccgctccgcc atccaggcga tcccggccgg
      901 tctgaccgta gatacgatcg gcgtcggccc cgcccgtgat ctggtcatcc ccgttgccgc
      961 cgaccagctt gccgacgcca tccccaccgg cgatgacgtc gttgccaccc tggccggtga
     1021 tcgagtcatc gccggagccc ccgatcagca agtcgtcacc cgctccgccc cggatcgtgt
     1081 tgcgaccgct tccgccattg accttgtcgt tgccggcgcc gagcgtcatg tcgtcgttgc
     1141 cagccccacc gcagacgacg tcattgccgc cgttgccggt gatcacgtcc cgaccggatg
     1201 agccgaggat gacgtcgtca cccaccgttc cggtgatgta ccgcgacccg cggatggtaa
     1261 ccacgtatga ctgtccagcg acgatgcagg ttgcggcgcg cgccggctgg gcaacagcaa
     1321 ttgccgatgc aaccagcacc atcaggacca cgagcacgcg catgccaacc gactccgcat
     1381 ccggaccacg acgaaacact gtccctgagt ctgtcgcaat ccgacacagg acgcagtacc
     1441 ggagtggggg agattcggac cgctctcgcc gggtcgtgcg tctggtcccg cattcgatgc
     1501 gggggcctca cacccggatg gaatcgcgcg tcataattga tgcacgcgga tctgctccca
     1561 tgaaaggaga cgcaccatga cccgcctatt ccgctggact gttgacctcg atgccctgac
     1621 cgacgaggcc tacgccttcg tgaccgaccc gcgcccctcg cgcaatgagc ggccggtgat
     1681 ccaggagttg cacgacatca acccggaaac cgtcgacgtg ctcgaatcga tgctgattga
     1741 cggtaccgag gagcgccggg acgtcgccga ctacatcgat gccgtcttcg gcccctacgc
     1801 cgcccgctag aaccaggccg gacccagacc atgcgtgccg gtttaccggt gggtcgatcg
     1861 caccaaatcc gagagatcgc ggcgggcgat tgccccggcc gcatccctac ggatgccatt
     1921 tgagaggaat gccatcgtca gctccgtgtc ccggtctgcc cagcacacgg atgagcccat
     1981 tccgccgtgc cagaacgtcc gctcagtact ggttgctcct ggccagtgac gccgggggtc
     2041 ggccaggccg ccaagctcga acgcgaggcc gcggcgtacc ggggcatcaa atgtgagatc
     2101 gatatcgccg tcgatttcga tctccatcat cctgtcgacg gtctccggtt ggagaatggt
     2161 cgcgccatcg atactgccgc ccgcggctat cgcggcatag aaccgcgcca tatcccgggc
     2221 ggacgaaacg ccgctggcac cgggaatcac catccgatag aacggtaccg accgggtttc
     2281 gcgcaaggca gtgaggctga gctcatcggt cccgtcggtg gcgtgcagct tcactacacg
     2341 tccatcgtgc atggctggca atcccacgaa ggtatcccgc aatccgagcg gaccggtgat
     2401 ctcgtcgtcc aggtaggcgt cgaaggtgcg gccatccagc ctccgtatca gttcgccgat
     2461 aacccagtgc tgcgtgagaa agtggtacgc gggcgcggtg ccgggcgggt gggccagcgg
     2521 catcctctcg atcgcccgat tcacgacggc ggcatcgccc cacatctcca tcgcgagctc
     2581 ggggggagtt gtcgggaatc cgccgcggtg ggacaggatg tgccgaacga gcacctcctc
     2641 cttgccattg actccaaacg ccggccagta ctcggctacg cgatcatcca gcgccagctt
     2701 gccgcgctcg atctgctgcc aaagcgcaac cgctccgaac ggcttcgtcc ccgaaaagag
     2761 ggggaagagc gagctggaat ccacgagttc gccgctttgc gtgtcggcat acccaccgac
     2821 cagatccagt gccaactctc cgtgctggta ggccgccagc gccgcgcccg ggaagaggcg
     2881 catcgggctg atctgggcgc gaaaagcgtc ttcgatcgcc tcaagactcg cggagtctgc
     2941 agcggggacg acttcgggtg ttgcaatcat ctcgatgtca ccttcagccc gccactgcca
     3001 gcgccacgcg ttcctgctcg actagcaccc ggcgcaggat cttgccggtc ggcgatttgg
     3061 gaatgcagtc gatgaactcg acgcgccgga ccttcttgtg gggagcaacg cgatcggcaa
     3121 cgaacgccat cagctcgtcc gctccggccg ggccgcgcaa gacgacgaac gccttcggga
     3181 cttcacccgt gtcgagatcg gggctgggaa tcacggccgc atcggcgacc gcgggatgcg
     3241 taagcagcac ggcctcgagc tcggccgggg ccacctggta ccccttgtac ttgatcagct
     3301 ccttgagacg gtcgacgatc gcgaacgacc ccgcctcgtc gacacaggca atatcgccgg
     3361 tgtgcaacca gccgtcctca tcgattgtgg tcgcggtcgc gtccggccgg ttgaggtacc
     3421 ccttcgtgat ctgggggccg cgcacccaca actcgccggg actgttcggg ccaagtgact
     3481 caccggacac caaatcgacg atcttcgcct ccgtattcgg gaccagcgga ccgacgctcc
     3541 cttcaatgtt ccggccagga atggtcatat gcgcaaccgg tgaggtctcg gtcagcccgt
     3601 atccctgttc gaccgcgcaa tcgagccggc tggcgcagtc gcgactcgtt cccgcactca
     3661 gtggagctgc gccgcaatta atgcgccgga gttgggtcac gtcgtagcgg tcgaccgcct
     3721 catgcgtcga caggatcaga acgaccggcg gcaccagcgc ggtgtaggtc acgccgtacc
     3781 gctcgatggc cgtaagaaac gattcgatct cgaagcgcgg cagcagcacc aacgttgcgc
     3841 ccaccgcgag gccgtgattg agtagtactt gcaggccgaa ggaatggaag atgggaagca
     3901 agcagagggc aacgtcgtcc tgaccatacg gacgcacggc cgcaagctgc tcgatattgg
     3961 ccaccaggtt ccggtgggtg agcatcacgc ccttgggaag cccggtcgtt ccgctcgaat
     4021 agagcagcgc gacggtgtca ttcgcggcat cgacgatcgc aaccggcggg tcgccaacgc
     4081 tttcagacac gtccgcgaaa ggaatcgtcc cgtctgcctt gccggtggag tagatctcgg
     4141 taatcccggc ccctcgcgct gccggacccg ccttcgccat taggtcaggc gtcgtcagca
     4201 ggcggctggc acccgaatcc ttgagctggt gctccagctc atcggcggtg taggccggat
     4261 tgactgtcgt catcacgccg ccgagccatg agaccgcgtg gaatgcgacc gcgtactcga
     4321 tgcagttcgc ggcatagata gcgaacacgt cgcccgcacg gaatcctcgc gcggcaagac
     4381 cggctgccgt gccgcgaatc gcccgggcaa gctcctgata ggtcatcgtg cgtccactca
     4441 ccccatcgat gagcgccggc ttcgcggcca gtcgctcggc atggcggagg ataaacggcg
     4501 tcagggcgat atcagggatt gcgatatcag ggtgcggact gcggatgatc atggaccgct
     4561 tcctttcgag gtctgacgcc gcccgcgact cctcacttgt gaggcgtggg tacgtcttcg
     4621 ccaaaccgca ttgaactgat atcagtctgc tggccccggg ctgcaccgtc gtccgctgat
     4681 ctgccgattt gcgcccacgc actcggccaa ccgcgccata cgtatacgta ggtcgacggc
     4741 aagtggcgga tctagatcaa tccgcgctcg accgcatccg cggcggcctc cgcccgggtt
     4801 ccgacatcga gcttgtcgag aatgtgctgg acatgcgtct gggcagttcg ctcgctgacg
     4861 aacaggcgct cgccgatatc gcgattggac aggccgtccc ggatgagccg cagcacttcg
     4921 acctctcgcg gtgtgaggcc gctccgcctc gcctgccccg gcgtggcggc cccccgcgcg
     4981 ttcgggccgt tacctccgag aattccggag gcgatctcgc gaagggctgt ctctgatccg
     5041 gccttcttcg ctgcggcgaa gtcaacttcg cccaaggctg atcgagcccg gtcaatctca
     5101 cagtcgtaac gatcatgatc tcgtggttct ggcgccgcga tctcgccacg caatgcttcc
     5161 gccgctccaa gcagccgcgc cgcatcggcg tgttttctgg tcagcaaggc gagggtagcc
     5221 agtccccgaa ggcagctgag gatccccggt ctatccccct gctcccagcg cagggccaga
     5281 ctatcgacca tcagcccgtg tgcttcggcg tattcgccgc gcctcctggc aaccctgccg
     5341 aggttcatca ggacaaggct ttcgccgtat gaatttccgg catcgcgaaa ctcggccagg
     5401 gactggcgga gattgggcac agcccgctcc aggtccccct gacggtaggc agccagtccg
     5461 aggctattga gcacgtatgc cggccagacg gcatctccat agctccggta gacggccaac
     5521 gcctcctcat gtcgcgcgac cgcttcgtcg acccggccca tgtcttcaag cacgaatccc
     5581 agcacggtca gactctgccc gtaccagtgt tgatcggccg tatcctgagc cagctcgatg
     5641 ccttcatcga tcatgcgctt ggcgctctca cggtcgccaa gctgaagcgc cagccatccc
     5701 ccaacgagca atgcatggcc gtaactttgc cgattggtgg gaccagccgc gagcgctcgc
     5761 tcgatccagc ttctaccctc ggcaaagtag ccacggacag accagtagcg ccccatcgca
     5821 accgccaaca actgtgctcg tgcggcctcc ccgttctcga gcagccagcc caacgcaatc
     5881 cggacattgt catactcggc gtcgaagatc ttcagccacg ttccttgctc cggaccgaac
     5941 atgtgctcga agccggagct gaacagttcc aggcaccacg aggacatcgc attcagcgcg
     6001 gcgtcactct cgccatacgc cacaagcagc tcgaacccga accggcgaat cgtctccagc
     6061 atcccgaacc ggggtgcggc cgaatcttcg acccggcgaa ccaggctctt ctcctccagc
     6121 gatgagatgc gctccagaat cgagccggct gggtccgaac cctcggcgaa caaggcttcc
     6181 gccgcctcga gtgagaaccc gccgccaaat atggacaact gccggaacag cgcctgctcc
     6241 tcgagcggaa gcaggtcata gctccagccc acggcgtcgc gcatggtgcg caagcgcgcc
     6301 ggcatgtccc gggcgtcccg gctaagcaga ttgaaacgcg tggccagcct gttcaggagc
     6361 tcctggggcg ggagcatctt gatgcgcggc gcggcgagct cgatcgcgag tggcaaccca
     6421 tccaggcggg agcagatggc cgctacagcc gcggcatttc cctcatcgag cgcaaagccc
     6481 ggttgtgctg ccctggcccg ctgcacaaag agagcgaccg catctgtgcc ggcgaccgat
     6541 tcggcggttg ctcgcgtgtc cacatcggga accgccagcg gcttgagcgg atactcctgc
     6601 tcgccttcga cccgaagcgt ctcacgactc gtcaccagca gcgtcaagct ggggcaggcc
     6661 cgcagcaagc tcgaaagcgc cggggcggcg ccgatgacgt gctccatgtt gtccagcacc
     6721 agcagcagct cgcgatcctg caggaaatcc cgcagtccga cttcggctga ctggccgccg
     6781 agcccggaga gcccgaggcc ctgcgcaatg gctggaatga cgaggtcaac atcggcgatg
     6841 gcggcgagag gaacgaaccg ggcatcgtct ctgaatgcgg cggcaaggtc tccagcgacg
     6901 tgcatcgcga gccgcgtttt gccgacaccg cccggtccgg tcacggtcac catcggaaca
     6961 tcggggcgga gaaggagatc gcgaaccgcg gcaagctcgc gctggcggcc aaccatcggc
     7021 gtccgcggaa caggaaggag cgcgcctggc tcggtagcgg atggaccgga cattgccgta
     7081 ccccgcactg gaatcgttta tcgggtataa cggtaatcct gtcacaatcg gcgcgagcgg
     7141 tcaaacgctc gcgattgggg cactgcgcgc cggctccgac tgcgtactag tccgtcgcag
     7201 ccagcaaccg ctcggccgca gtctggagca gccaaaggcc gttggcgtcg atctcccgtt
     7261 gcgtgtcgtt gatcaccgcc gagaaggcga accagcgccc atcctgccgc tgcaagagcc
     7321 aggaattgct catcgcgccg gtctcgtagc cgcccttgta cccgacgtat cgccaggtgt
     7381 cggggtgcca gcagctgccg gggttgagcg aaagggcatc ccgcaccggt gcaccctcaa
     7441 caccgtccga acgccgccag agccactcga tcgcccggac cagatcggcc ccgccggcga
     7501 accactcgat ccgatcgagg aagcgcggcc ccggccacgg ctcggcatcg ctgagttgcg
     7561 cggccagcgg gtcgaccgtg ttggcgagaa tgcgcaaccg cttgccggca tccgccgcca
     7621 ggtagcgctc gatctgcgta tcgcgcagcc gcatccggat cgcaaaccac tcccgcgtca
     7681 tcaggagcgg ccggttggca tccggccggc tatggccttg gtcggcaaac ccggcttgaa
     7741 tccggggccg gccgagtcgt ccgatcaggt ggtcggtcgc ggtgttgtcg ctgccggcga
     7801 tcatccgctc ggcgaagtgg cgaatcggca actccagccc ggttggcagc agccgcatat
     7861 cgccgttcgg caacgagcgg agatcgtcgc ggatcgcca
//
LOCUS       DASSGX010000363         2287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1590251, whole genome shotgun sequence.
ACCESSION   DASSGX010000363 DASSGX010000000
VERSION     DASSGX010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590251"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..787
                     /locus_tag="VFI12_06445"
     CDS             95..787
                     /locus_tag="VFI12_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056083.1"
                     /translation="MSLSYAETLRNLGYCDTGGNSRTIKKYVDLWEVDVAHFNPDFAR
                     LKALKNPRRPIQEVLVANCTYSRGTLKSRLFSEGLKARNCEFCGQGEQWRGQRMGLIL
                     DHINGVRDDNRLDNLRILCPNCAATLDTHCGRKNRIPKEARSCLRCGTDFFPKYETHR
                     YCSRNCGQRVGATASRPAARRVERPPHVQLMAEIEATSYLAVGRKYGVSDNAIRKWVR
                     AYEREAGEGEGD"
     gene            816..2204
                     /gene="glmU"
                     /locus_tag="VFI12_06450"
     CDS             816..2204
                     /gene="glmU"
                     /locus_tag="VFI12_06450"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HET7056084.1"
                     /translation="MSAPTVLIMAAGQGTRMKSAVPKVLHPICGRALVSWPIEAARQA
                     GAGRVAVIVSPDRDISGALPEGVESIPQPEANGTGGALLAAAEAVAESETVLVLSGDV
                     PLVSAELIAEIVATHEREGAAATVVTAVLDDPGAYGRVVRSADGAAIERIVEAKADGD
                     ATEAELAINEINAGIYVFDGPALAAALPELSNDNAQGEYYLPDTLPLLRAAGGRIAAH
                     ISADPNVILGVNSRADLALVEAEARRQILERHMLAGVTIVDPASTRIEVEVDIAADVR
                     IEPGCSLIGTTTIGTGSVVGPLTTLIDSRLGAAVNVPHSYLLECEVADRCTVGPFAYL
                     RPRAELAEGAKAGTFVEIKNSRIGAGTKVPHLSYIGDADVGEGANLGAATITANYDGY
                     VKNRTKIGHGARTSVDTTLVAPVEIGDSAYTGAGSVIDEDVPPGALGIARARQTNIEG
                     FAERKAKENEQK"
BASE COUNT          417 a          766 c          761 g          343 t
ORIGIN      
        1 ggccgaaaac ggggccgtcc ggagggcgaa ctagtgttcg tgtatgaggc gacggccggc
       61 gttcacacag aaagacctgc aagaggcagt cgaggtgtcg ctcagctacg ccgagaccct
      121 tcgcaatctc ggctactgcg ataccggggg caacagcagg acgatcaaga aatatgtcga
      181 tctatgggag gtcgacgtgg cccatttcaa cccggatttc gccaggctga aggcgctcaa
      241 gaaccctcga cggcccatcc aagaggtact ggtcgccaac tgcacctaca gccgcggcac
      301 cctcaagagc aggttgttct cagaggggct gaaagcgaga aactgcgaat tctgcggcca
      361 aggtgagcaa tggcggggcc agcgcatggg gttgattctc gaccacatca acggcgttcg
      421 cgacgacaac cgtttggaca acctcagaat tctctgcccg aactgtgcgg cgacgcttga
      481 tacccattgc ggacggaaga accgaatccc taaggaggcg cgttcttgtt tgcggtgcgg
      541 gacggatttc ttccccaagt acgagacgca tcgctactgc tccagaaatt gcggtcaacg
      601 ggttggcgca acggcctctc ggccggctgc ccgacgggtt gagcggccgc ctcacgtgca
      661 gctgatggct gagatcgagg cgaccagcta tctggccgtc ggtcgcaagt acggcgtctc
      721 cgacaacgcg atccgcaagt gggtgcgtgc atacgagcgt gaggctgggg agggggaggg
      781 cgactgagct tggccgccgc aatacgatgc gctgagtgag cgctccgacc gtcctgatca
      841 tggccgccgg tcagggaacc cggatgaagt ccgcggtgcc caaggtgctc catccgatct
      901 gcggccgcgc cctggtctcc tggccgatcg aagccgcgcg ccaggcgggc gccgggcggg
      961 tggccgtgat cgtctccccg gatcgcgaca tctcgggagc gctgccggag ggggtcgagt
     1021 cgatccccca gccggaggcg aacgggaccg gcggcgcgct gctggccgcc gccgaggcgg
     1081 tggccgaatc ggagaccgtc ctggtcctct cgggcgacgt ccccctggtc tccgcggagc
     1141 tgatcgctga gatcgtcgcc acccacgagc gtgagggcgc agcagccacc gtcgtcaccg
     1201 cggtcctcga cgacccgggc gcctacgggc gcgtcgtccg atccgccgac ggtgctgcga
     1261 tcgagcgcat cgtcgaggcc aaggccgacg gggatgcaac cgaggctgag ctggcgatca
     1321 acgagatcaa cgccggcatc tacgtgttcg acggtccggc cctggcagcg gcccttcccg
     1381 aactctccaa cgacaacgca cagggcgagt actacctgcc cgacacgctg ccgctgctgc
     1441 gggcggcggg gggtcgcatc gccgcgcaca tctcggccga cccgaacgtg atcctcggag
     1501 tcaactcccg cgccgacctg gcgctggtcg aggccgaggc ccgccgtcag atcctcgagc
     1561 gccacatgct cgccggagtc acgatcgtcg atcccgcctc gacccggatc gaggtcgagg
     1621 tcgatattgc cgccgacgtc cggatcgagc ccggctgctc gctgatcggc accaccacga
     1681 tcggcaccgg gagcgtcgtc gggccgctga ccaccctgat cgattcacgg ctcggcgccg
     1741 cggtcaacgt gccacattcc tacctgctcg agtgcgaggt ggccgatcgc tgcacggtcg
     1801 gcccgttcgc ctacctgcgc ccccgcgccg agctcgccga gggcgcgaag gccggcacgt
     1861 tcgtcgagat caagaactcg cggatcggcg ccggcacgaa ggtgcctcat ctctcctaca
     1921 tcggcgacgc cgacgtcggg gagggtgcca acctcggcgc cgcgacgatc acggccaact
     1981 acgacggcta cgtcaagaac cggacaaaga tcgggcacgg tgcgagaacc agcgtggaca
     2041 cgacactcgt tgcccccgtt gagatcggcg attccgccta cactggcgcc ggttcagtga
     2101 tcgacgagga cgtgcccccg ggcgccctcg gcatcgcccg ggccaggcag accaacatcg
     2161 agggtttcgc cgagcggaaa gccaaggaaa acgagcagaa gtgagccagc ccggcaagat
     2221 gacgaccctt tcgcatcctc cctccgacca ggacgagacg gatccgcggg cggtcacctc
     2281 gatccag
//
LOCUS       DASSGX010000364         4575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1261889, whole genome shotgun sequence.
ACCESSION   DASSGX010000364 DASSGX010000000
VERSION     DASSGX010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261889"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VFI12_06455"
     CDS             <1..208
                     /locus_tag="VFI12_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056085.1"
                     /translation="MTIDVENRTLSVPEIGVEVEFPMDDFTQHRLLNGLDDIGLTLQH
                     QNDIAAFEARRPGFLPVTIGGEAR"
     gene            205..987
                     /locus_tag="VFI12_06460"
     CDS             205..987
                     /locus_tag="VFI12_06460"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HET7056086.1"
                     /translation="MIRVSVVELPDNRDRFELAWPALLQHVSFAESDVIVLPELPASS
                     WFGVTPSFDQSRWDRIVAAHDALVSRLDDFGSAIVIGSRATIVGDQRQNVAFVWSKET
                     GLIDLHPKAILPEEPGFHEQSWYHAGTDDLRVVEVRGITIGVLLCSELMHTERARKLG
                     NAGATIIGVPRATGDHPRWQIASQMAAVTSGAFVLTSNRSGQAQGDPSVIFGGRSMIV
                     DPDGAILSETGRDKSFASASVDLGASRAARKSYPRYLDYGDR"
     gene            1006..2106
                     /gene="leuB"
                     /locus_tag="VFI12_06465"
     CDS             1006..2106
                     /gene="leuB"
                     /locus_tag="VFI12_06465"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HET7056087.1"
                     /translation="MSKPKIDLVLLPGDGVGPEIIAAAQAVLSAVERRFQISVETDSR
                     LIGGAAIEAHGTPLRAEDLERCNLADAVLLGAVGGPKWDDPKATVRPEQGLLRLRKGM
                     GLFANLRPVAVMASLVDRAPIKPELLTGVDMMVVRELTGGVYFGRPRKSWRERRGRAA
                     LDTLIYREYEVERISRLAFEIARDRKGKLASVDKANVLDTSRLWRSVVTEMAPEYPDV
                     ELEHVLVDSMTMHLIREPARFDVIVTENMFGDILTDEASVLAGSIGMLPSASIGAPRS
                     GSDSRRRGVYEPIHGSAPTIAGKGLANPVGTILSLAMLFRISLQRDDVGSAIETAVST
                     VINSGKRTPDIADIDSTVVSTGELGSAIAQAI"
     gene            2190..2552
                     /locus_tag="VFI12_06470"
     CDS             2190..2552
                     /locus_tag="VFI12_06470"
                     /inference="COORDINATES: protein motif:HMM:NF017920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HET7056088.1"
                     /translation="MKLVVAIIQDYDTDAFLKAVSMHNLMATRIASAGGFLRTGNTTV
                     FMGVEDDEVPLVKRLLVEHCRIREQQAVAVEFPYVDDADFEHISVSRIGGGVAFVARI
                     ERFEKFARATNDPSETTA"
     gene            complement(2557..2826)
                     /locus_tag="VFI12_06475"
     CDS             complement(2557..2826)
                     /locus_tag="VFI12_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056089.1"
                     /translation="MIGKIEKAHRYAREPERVRLNSLNATFHGGHDEYTIELKDGHWT
                     CSCHTFSSHAVGTCAHVMALQQMLGEMLPADLRYDVAEQEHAAVV"
     gene            3108..3743
                     /locus_tag="VFI12_06480"
     CDS             3108..3743
                     /locus_tag="VFI12_06480"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HET7056090.1"
                     /translation="MDSEQAKVLNSEVTVAILAGGKSARFGSDKALASIPGEPVSFLE
                     RAVVVARVVADQVIVIGPTRPGVDRLDLRFVPDRFPGEGPAGGVITALVGVATARLVV
                     LACDQPLIAPLDLIALLEDGRGHPAVAFASSDGYLQPLPCYLDVATCRDMAISAFDSG
                     SRSLREILQTCGVASIPSDRNGRLADIDTVDDYHALADSIAPGRRDGTDSV"
     gene            complement(4213..>4575)
                     /locus_tag="VFI12_06485"
     CDS             complement(4213..>4575)
                     /locus_tag="VFI12_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056091.1"
                     /translation="MAGGNTYEIYALGQSAAGLRIILRETFRDPSNADRVSFPRQISQ
                     AGKYLRERDLLRQRDEADFYFSDEEEEEGEVEAEGEAAETPDEDEENVAEGEEFITEG
                     EEPEEAEEDAEADEDEEL"
BASE COUNT          775 a         1425 c         1474 g          901 t
ORIGIN      
        1 gatgacgatc gacgtcgaaa accggacact ctcggtcccg gagatcggcg tcgaggtcga
       61 gttcccgatg gacgacttca cgcagcatcg gctgttgaat ggcctcgacg acatcgggct
      121 gacgctccag catcagaacg acatcgcggc attcgaggcg cgccgtcctg gctttctgcc
      181 ggtgacgatc ggcggtgagg cccggtgatc cgcgtctcag tcgtcgagtt gccggacaac
      241 cgggatcggt tcgagctcgc ctggcccgcc ctgcttcagc atgtcagctt cgccgaaagc
      301 gacgtgatcg tcctgccgga gctgcctgcc agctcctggt tcggagtcac gccctcattc
      361 gaccagtcga gatgggatcg catcgttgcg gcgcacgatg cgctagtctc ccggctcgac
      421 gatttcggga gtgcgatcgt gatcggctcc cgcgcgacca ttgttggcga tcagcgccag
      481 aacgtcgcat tcgtctggtc gaaggagacc ggactgatcg acctgcatcc gaaggcgatc
      541 ttgcccgaag agccggggtt ccatgagcaa tcctggtacc acgctggaac cgacgatctg
      601 agggtggtcg aggttcgcgg gatcacgatc ggcgtcctgc tctgttcgga attgatgcat
      661 accgagcggg cgcgaaagct tggcaatgcc ggcgcgacca tcatcggcgt gcctcgcgcg
      721 acgggcgatc atccacgctg gcagatcgcc agccagatgg cagcggtcac atccggcgca
      781 ttcgtcctga cctcgaatcg atccggccag gcacagggcg atccgtcagt catcttcggc
      841 ggccgttcga tgatcgtcga ccccgatggc gcgatcctca gcgaaaccgg gcgagacaag
      901 tcattcgctt ccgcctcggt ggacctcggc gcaagccggg ctgctcgcaa atcgtatcca
      961 cgctatctcg attacggcga ccgctagtcc ctggagcagt gccccttgtc aaagcccaag
     1021 attgaccttg tcctgctgcc cggtgatggt gtcggcccgg agatcatcgc tgcagcgcaa
     1081 gccgtgctgt ctgccgtcga acgccgcttt cagatttcgg tcgagaccga ctcccgattg
     1141 atcggcgggg cggcgatcga agcgcatggc acgccgcttc gggctgagga tcttgagcgg
     1201 tgcaacctgg cggatgcggt gctgcttggc gcggtcggtg ggccgaagtg ggacgatccg
     1261 aaggccacgg tccggccgga gcagggcctg ctccgtctcc gcaagggtat gggcctcttc
     1321 gcaaacctgc gaccagtagc cgtgatggca tcgctcgttg atcgggcgcc gatcaaaccg
     1381 gagctgctca cgggtgtcga catgatggtc gtccgggagc tgaccggcgg cgtctatttc
     1441 ggccggccgc gcaagtcgtg gcgggagcgc aggggtcggg cggcgctcga tacgctgatc
     1501 taccgcgaat acgaagtcga gcgcatctcc cggctcgcgt tcgagatcgc gcgcgaccgc
     1561 aagggtaagt tggcttccgt cgataaggcg aatgtgctcg acacgtctcg cctctggcgg
     1621 tcggtcgtga ccgaaatggc gccggagtac cccgacgtcg agctggagca cgtgctggtc
     1681 gattcgatga ccatgcacct gattcgcgag ccggcgcggt tcgatgtgat cgtaacggag
     1741 aacatgttcg gtgatatctt gaccgacgag gcgtcggtgc tggccggctc aatcggtatg
     1801 ctgccctcag caagtattgg cgcgccgcgg tcggggagtg actcgcggcg tcgcggtgtc
     1861 tacgagccga tccatggcag cgccccgacg atcgccggca agggactcgc caatccggtt
     1921 ggcacgatcc tgtcgttggc gatgctgttc cggatatcgc tccagcggga cgacgtcggt
     1981 tcggcaatcg aaacggcggt ctcgaccgta atcaactcgg gcaagcgaac accggacatc
     2041 gccgatattg attccacggt tgtctcgacc ggcgaactcg ggtcggcgat cgcgcaggca
     2101 atctgaaacg gggaggccgc aatgacgcgg aaaatggcga tcacgacgat gtggcgcgcg
     2161 ggttttggca gcaatcgcga gcaggcgcga tgaagctggt cgtcgcgatc atccaggact
     2221 acgacaccga tgccttcctc aaggcggtca gcatgcacaa tctgatggca acccggatcg
     2281 ccagtgccgg tgggttcctt cgcaccggca atacgaccgt cttcatgggt gtcgaggatg
     2341 atgaagtgcc gctcgtcaag cgactgctcg tcgaacactg ccgaattcgg gagcagcagg
     2401 cagtagcggt ggagtttccc tacgtggacg atgccgattt cgagcacatc tcggtctccc
     2461 gcatcggcgg cggtgtcgcg ttcgttgccc ggatcgagcg cttcgagaag ttcgcccggg
     2521 caacgaacga tccgtcagag acgacggcgt gacgggtcag acgacggccg cgtgttcttg
     2581 ctccgcgacg tcgtagcgca gatccgccgg cagcatttcg ccgagcatct gttggagcgc
     2641 catgacgtgg gcgcaggtgc cgacggcatg cgacgagaac gtgtggcagc tacacgtcca
     2701 gtgcccgtcc ttgagttcaa tcgtgtattc gtcatgacca ccgtggaatg tcgcattcag
     2761 actattgagg cggacgcgct cgggttcgcg ggcatagcgg tgcgcttttt cgatcttgcc
     2821 gatcatgctt gagtgcatct ctgcagaccc cctcgttgtt cgatctcccg accgagctgt
     2881 atcacgagcc cgaactcccg cgacatcgcc gccaaaagca aagcggcgca ggtctcaatg
     2941 acactgcgcc gctctcaagg acgtctttcg gttgcgatgt ttcgttggca agatcgcatc
     3001 ctccccggag ctctctcggt tccaacgtgg gcgaggcacc gttgcctcgg actgctcgtt
     3061 ccagacacta taccccggac aggcgggtga gaaaaggggc cggacgggtg gatagtgagc
     3121 aggcgaaggt cctgaattcc gaagtgacgg tggcgattct ggctggtggc aagagtgctc
     3181 gttttggctc cgacaaggcg ctcgcatcca tcccgggcga gcccgtctcc ttcctcgagc
     3241 gggccgtggt ggtagcccgc gtcgtcgccg atcaggtgat tgtgatcggt ccgacacgac
     3301 cgggtgtgga tcgtctcgac cttcgcttcg tcccggatcg gtttccgggc gagggaccgg
     3361 ccggcggcgt gatcaccgcg ctcgtgggtg tcgccaccgc aaggctggtc gtgctcgcat
     3421 gcgatcagcc gctgatcgcg cccctggacc tgatcgccct gctcgaagac gggcgcggcc
     3481 atccggcggt cgcgttcgcg tccagtgacg gctatctgca gccgctcccc tgctatctcg
     3541 atgtcgcaac ctgtcgtgac atggcgattt ctgcgttcga ctccggctcg cgatcgcttc
     3601 gggagatatt gcagacctgc ggtgttgcat cgatcccctc cgaccggaat ggtcggcttg
     3661 ccgacatcga cacggtagac gattatcacg ctcttgcgga ttccatcgca ccaggacgca
     3721 gggacgggac ggattcggtc tgatggcatg ctggttcgtc gctatacttg gcacgcatca
     3781 gtttgagcgc agcaaccaag gatcaacgta acgcaatgtc tcaatccact tccagtcgcc
     3841 agcgacgcca ggcgcaagcc cgcaaaaatg cacggggtgc ggctcgcccg aaccaatcac
     3901 agttgcgggc ggcggagatc cgggcagccg agagcacggc ggttgccgcg actccaactg
     3961 caaccgtaac cactgccact gcccgccccg gacgtggtcg gtcggctgcc cgtgggattg
     4021 gactgagccg cgcggcggag atgcggtatc agcgcaacga cctgcggcga ctgctctaca
     4081 cggccggtgt tctcttcgtc ttgatgattg tcctgctctt tatcctcgac tgagcacgat
     4141 cactgtcacc acaaacgaga acgggcgggt ggatcgtttc cacccgcccg ttctcgttcg
     4201 caggaagagt ggctagagct cttcgtcctc gtcggcttca gcgtcttctt cggcttcctc
     4261 gggctcctcg ccctcggtaa tgaactcctc gccctcggcg acgttctctt catcctcgtc
     4321 cggtgtttcg gcggcctcgc cttcggcctc gacctcgcct tcctcttcct cctcgtccga
     4381 gaagtagaaa tcggcctcgt ctcgctggcg aagcaggtcc cgctcgcgga gatacttgcc
     4441 ggcctggctg atctggcgcg ggaatgagac acggtcggcg ttcgagggat cgcggaaggt
     4501 ctcgcgaagg atgatccgca ggcctgccgc cgactgtccg agcgcgtaga tctcgtacgt
     4561 gttgccgccg gccat
//
LOCUS       DASSGX010000365         4067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1154696, whole genome shotgun sequence.
ACCESSION   DASSGX010000365 DASSGX010000000
VERSION     DASSGX010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4067
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154696"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..1367
                     /locus_tag="VFI12_06490"
     CDS             141..1367
                     /locus_tag="VFI12_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056092.1"
                     /translation="MITLNPGVTLRGGTLKFGAKGVRLTRDNILDGVTVLTEDDEIAI
                     LNDTGVSDLGTLTLRGIRVTGQVLLLAEDAVRRGHVLVDGLTIDRADVRGRSERPHGF
                     GVEALHGAFTLWNRQADPDVVITAELLNISAGSADAPVRGSGVFVGGHGNRDGMADGG
                     TVRVTTLRTGEIHADGRIPPGTPDLISGGVFVISGAEVEEVINEGPVTTYGQNDMVLD
                     NWGSVATWTASAPVTSRGPSGIGFVNFGDIDRLDVQAPIQTYGTGARGFNLYEGSLQH
                     ASFTSIATHGDGSVGVQVSKPLPVLDISGDLSTEGGEGQSLVKGVQTTLKAIALSVKP
                     GGDIDRVSIGGQIRTAGDDVVTVEIEGDVGELVAAGGIIARGQNSDAIHTRNHIAGLD
                     QLTVHAEHGQDVVRAG"
     gene            1399..1833
                     /locus_tag="VFI12_06495"
     CDS             1399..1833
                     /locus_tag="VFI12_06495"
                     /inference="COORDINATES: protein motif:HMM:NF017663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HET7056093.1"
                     /translation="MGTLRLTLPLPPSINSQYVTVGRRRVLSKDAKSFKSDVKKLIGN
                     LRTTGKIPIQLEAELQKSLLGVYLTFHFETPFRRDLDGGLKIALDSLAEALGFDDRMV
                     VDLHLVKQMDTLNPRLEVEIETIADWQFDPQYVLLEPAQELE"
     gene            1884..3461
                     /gene="guaA"
                     /locus_tag="VFI12_06500"
     CDS             1884..3461
                     /gene="guaA"
                     /locus_tag="VFI12_06500"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HET7056094.1"
                     /translation="MTETLDSSTPAAVEPVATEMVAVLDFGSQYSQLITRRVREAGVY
                     SELFHHDTPWSEIESRNVRGVILSGGPASVYEAGAPQLPDWVLKRGLPVLGICYGMQL
                     IAQALGGSVEPAERREYGPARMWVTSKDSPLFAHLPDAIDVWMSHGDHVAKMPDGFTT
                     IGQSDNAPCAAIARGKVVAIQFHPEVAHTPLGKVVLRNFLTEICGLQPTWTPASFVEH
                     AIAQIRLQVGTGLVLLGLSGGVDSSVAAALIHRAIGEQLTPVFVNNGLLRLGEADLVQ
                     DVFARAFGMNLVYADASARFLERLKGVTDPEQKRKAIGETFIRVFEAEAGKAGPFQFL
                     AQGTLYPDVIESTTKDTKAAVKIKTHHNVGGLPEDMQFELVEPLKYLFKDEVRAVGRE
                     LGLPDEVVDRQPFPGPGLAVRILGEITSHDLELLRHADAIIRHEIEESGEDVWQFFGV
                     LLPVNSVGVMGDYRTYGRVCAVRAVTSEDAMTADWARLPYDVLGRISNRIVNEVRGIT
                     RVVYDITSKPPATIEWE"
     gene            complement(3639..>4067)
                     /locus_tag="VFI12_06505"
     CDS             complement(3639..>4067)
                     /locus_tag="VFI12_06505"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HET7056095.1"
                     /translation="RLMEEINRILRLDQTWDVALVGFGNLGRAIAQYGGFVPSTFRIA
                     CIFDRNEDRIGQDVDGIVIQSDAEIAESSRRLGVKIGIIAVPQASAQIVADALIAGGI
                     EAILNYAPCHLRTPELVTVRDVDPVGAMQSMTYYLSSNDN"
BASE COUNT          717 a         1341 c         1328 g          681 t
ORIGIN      
        1 gatggactcg cgtcgaagcg ggtttcatgg tgagcactat gagcaaatct accgtcgtgg
       61 attcagccgc cagtttgctg gacgcgatcc agtctgatgc gagagaaatc gagatcaggg
      121 gcaccatcag cggcatgccg atgatcaccc tgaatcccgg cgttaccctg cgcggtggca
      181 cgctcaagtt cggcgcgaag ggcgtgcgcc tgacccgcga caacatcctc gatggcgtca
      241 cggtgctgac cgaggacgac gagatcgcca tcctgaacga caccggcgtc tccgatctcg
      301 gcacgcttac gctgcgcgga atccgtgtga ccggtcaggt cctgctgctc gccgaggacg
      361 cggtccgacg gggtcacgtg ctcgtcgatg gactgacgat cgatcgcgct gatgtccggg
      421 ggcgctcgga gcggccgcac ggattcgggg tggaagcgct ccacggcgcg ttcaccctct
      481 ggaaccggca agccgatccg gacgtggtga tcaccgccga gctgctcaac atcagcgccg
      541 gttcagccga tgccccggtg cgcggcagcg gcgtgttcgt cggcggtcac ggcaaccggg
      601 atggcatggc cgacggcggc acggtccggg tgacgacact tcggaccgga gagatccacg
      661 ccgacgggcg cattcccccc ggtacgcccg acctgatcag cggcggcgtc ttcgtcatct
      721 cgggcgctga ggtggaggag gtcatcaacg agggtccggt cacgacctac ggccaaaacg
      781 acatggtcct cgacaactgg gggtccgtcg caacctggac ggccagtgcg ccggtgacct
      841 cgcgggggcc gagtgggatc ggattcgtca acttcggcga catcgaccga ctggatgttc
      901 aggcgccgat ccagacctac ggaaccggcg cccggggctt caacctgtac gaagggtcgc
      961 tccagcacgc ctccttcacc agcatcgcga cccacggcga cggctctgtc ggcgtccagg
     1021 tcagcaagcc gctgccggtg ctcgacatct ccggcgacct ctcgacggag ggcggcgaag
     1081 gccaaagtct cgtgaagggt gtccagacga cgctcaaggc gatcgccctg agcgttaagc
     1141 ccggtggaga catcgaccgc gtctcgattg gcggtcagat acgcaccgcc ggcgacgatg
     1201 tcgtaacggt cgagatcgag ggcgatgtgg gcgagctggt tgcggcaggc ggcatcatcg
     1261 ctcgcggcca gaactcggat gcgatccata cccgcaatca cattgccggc ctcgatcagc
     1321 tcacggttca cgccgagcac gggcaggatg tcgtccgagc cggctgagcg tccgcagccg
     1381 ccgtgggaca attcatccgt ggggacgctt cgactgacat tgccgctgcc gccgagcatt
     1441 aatagccagt acgtgaccgt cgggcggcgg cgcgtcctca gcaaggacgc gaagtccttc
     1501 aagtccgatg tcaagaagct gatcgggaat ctccgcacaa ccggcaaaat cccgattcag
     1561 ctcgaggcag agctgcagaa gtcgctgctc ggcgtctacc tgaccttcca cttcgagacg
     1621 ccgttccgcc gcgacctcga cggcggcctc aagatcgcgc tcgattcgct cgccgaagcg
     1681 ctcgggttcg atgaccggat ggtggtggac ctccacctcg tcaagcagat ggacacgctc
     1741 aacccgcgcc tggaggtcga gatcgagacg attgccgact ggcagttcga cccgcaatat
     1801 gtcctgctcg aaccggcgca ggaactagaa taggcgcagt atccggcgct cgccgccacg
     1861 cccctccgga ggaatgcccg cccgtgaccg agaccctcga ttcgtcgacc cccgccgccg
     1921 tggagcccgt cgccaccgag atggtggccg tgctcgattt cggctcgcag tacagccagc
     1981 tgatcacccg gcgggtgcgc gaggccggcg tctacagcga gctctttcac cacgacacgc
     2041 cgtggtctga gattgagtcg cgcaacgtac gtggcgtcat cctctccggc ggaccggcca
     2101 gcgtctacga agcgggtgcg ccgcaattgc ccgactgggt cctgaagagg gggctgccgg
     2161 tgctcggcat ctgctacggg atgcagctga tcgcgcaggc actcggcgga tcggtcgagc
     2221 cggccgaacg gcgcgagtac ggtccggccc ggatgtgggt gacctcgaag gattcgcccc
     2281 tcttcgccca tttgcccgac gcgatcgatg tttggatgag ccacggcgac cacgtcgcga
     2341 agatgccgga tggcttcacg acgatcggcc agagcgacaa tgcgccctgc gccgcgatcg
     2401 cccggggcaa ggtcgtcgcg atccagttcc acccggaggt cgcccacacc cccctcggca
     2461 aggtggtcct gcgcaatttc ctgaccgaga tctgcggcct ccagccgacc tggacgccgg
     2521 cctccttcgt cgagcatgcc atcgcgcaaa ttcgattaca ggtcgggacc ggactggtcc
     2581 tgcttggctt gagcggcggg gtcgattcct cggttgcggc cgctctcatc caccgggcga
     2641 tcggcgagca gctgaccccg gtcttcgtaa acaacgggct gctgcggctc ggcgaggcgg
     2701 atctggtcca ggacgtcttc gcccgcgcct tcggcatgaa cctggtctac gccgacgcat
     2761 cggcccgctt cctcgaacgg ctgaagggcg tgaccgatcc cgagcaaaag cggaaggcga
     2821 tcggcgagac cttcatccgc gtcttcgagg ccgaagccgg gaaggccggc cccttccagt
     2881 tcctggccca gggcacgctc tatccggacg tgatcgagag cacgaccaag gacacgaaag
     2941 ccgcggtcaa gatcaagacg caccacaacg tcggcggtct gcccgaggac atgcagttcg
     3001 agctggtcga gccgctcaag tacctgttca aggatgaggt ccgcgccgtc ggccgcgaac
     3061 tgggcctacc ggacgaggtt gtcgatcgcc agccgttccc gggtccgggc ttggccgtcc
     3121 gcatcctcgg cgagattacg tcccacgatc tggagctgtt gcgccacgcc gacgccatca
     3181 tccgccacga gatcgaggag agcggcgagg acgtctggca gttcttcggc gtcctgctac
     3241 cagtcaattc cgtcggcgtg atgggcgatt accggacata cgggcgggtc tgtgcggtcc
     3301 gggcggtgac cagcgaggac gcgatgaccg ccgactgggc gcgcctgcca tacgacgtcc
     3361 tcggccggat cagcaaccgg atcgtcaacg aagtgcgcgg catcacccgc gtcgtctacg
     3421 acatcacctc caaacctccc gccaccatcg agtgggagta ggattggccg cgttggcgcc
     3481 agctccgccc aggaatggat tcctggactc aggtacggat gttggttgaa accgaccatt
     3541 tcgacgtggg gtgaaccagc gatgcccaac cagtcgcgga gaggcttcgt gtccttggcc
     3601 cggggtttca accccgggag cactccgtcg cgcagaacct agttgtcgtt gctcgagagg
     3661 taataggtca tcgattgcat cgcgccgacg gggtcgacgt cgcggacggt gaccaattcg
     3721 ggcgtgcgca agtggcaggg cgcgtagttg agaatcgcct cgatgccgcc cgcaatcagc
     3781 gcgtcggcca cgatttgcgc ggacgcttgc gggacggcga tgatcccgat cttgaccccg
     3841 agccggcggc tcgactcggc gatttccgcg tcgctctgga tcacgatgcc gtcgacatcc
     3901 tgcccgatcc ggtcctcgtt gcggtcgaag atgcaggcga tccggaaggt ggatggtacg
     3961 aacccgccgt actgcgcgat cgcccggcca agattaccga aaccgaccag cgccacgtcc
     4021 cacgtctgat ccagccgcag gatgcggttg atctcctcca tcagccg
//
LOCUS       DASSGX010000366         2102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_827546, whole genome shotgun sequence.
ACCESSION   DASSGX010000366 DASSGX010000000
VERSION     DASSGX010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827546"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VFI12_06510"
     CDS             complement(<1..352)
                     /locus_tag="VFI12_06510"
                     /inference="COORDINATES: protein motif:HMM:NF024313.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056096.1"
                     /translation="MSADATLETDTQAAAAAPDRASAAELATLAPDLTSRPARSPAQI
                     ALARFRRSKLAMLGLLMIGLLTLAAIFAGTIAPYGENEIDLFHITAPPSAAHWLGTDD
                     LGRDELSRLIYGARV"
     gene            complement(381..1334)
                     /locus_tag="VFI12_06515"
     CDS             complement(381..1334)
                     /locus_tag="VFI12_06515"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7056097.1"
                     /translation="MTRFLIRRLLGAIPLLLGVAVLSFIFMQLAPGGPDALFARNARM
                     SEEQLQAIRHNMGLDRPMHEQLLIWLGNLARGNLGISYSQYRPVSEVIWDTVPNTLLL
                     MGTGMLISLAAALTFGILAARRPYGFFDNIVSFVSYFGLAMPVFWFGLMLQILFAVRL
                     GWLPSAGMYSATGGGGPGDLVRHLALPAFTIAIGSIAGWSRYVRSSTIEALGEDYVRT
                     ARAKGLAERRLLMGHVLRNALIPFVTVVGIDVPLYLTGAVLTETVFSWPGMGRLFYDA
                     LTVRDYPILMGVLMLGAVFIVAGNLIADILYGVCDPRISYG"
     gene            complement(1399..>2102)
                     /locus_tag="VFI12_06520"
     CDS             complement(1399..>2102)
                     /locus_tag="VFI12_06520"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7056098.1"
                     /translation="IADQAPGSWAFNPNIEPRPYDLEQAKALLADAGLTPGADGVLEG
                     PVPTDDPNVGDGEVKPLEIELWFVSGNVDSERISQVVAQSWNSIGVKTTVLNQDISTI
                     WGPEGYQFTEKMTGCLFAWVNGNDPTDTFYWHSSQIPETPTGSGGNLPAYFHQYSFQD
                     KIDELTTAGDTTTDQDERQKIYWQIQELLHEEVPVTFIYWEKSFPAVYNTIGGFWPSA
                     FNNLLWNAQEWYLTK"
BASE COUNT          360 a          667 c          719 g          356 t
ORIGIN      
        1 agacccgcgc tccgtagatc agccggctca gctcgtcgcg tcccaggtcg tcggtgccga
       61 gccagtgcgc ggccgagggt ggcgcggtga tgtgaaagag gtcgatctcg ttttccccgt
      121 agggggcgat cgtcccggcg aagatcgcgg cgagcgtcaa taggccgatc atgagcagac
      181 cgagcatggc cagcttcgag cggcgaaacc gcgccagtgc gatctgggcc gggctgcgcg
      241 ctggcctgct ggtcaggtcc ggggccaacg tcgccaattc ggcggcgctg gccctgtcgg
      301 gcgcagcggc ggctgcctgt gtgtcggtct cgagcgtagc gtcggcgctc atcgcccgtt
      361 ccccttctgc ctgcggcggc tcatccgtag ctgatccggg ggtcgcatac gccgtagagg
      421 atgtcggcga tcagattgcc agcgacgatg aagacggccc cgagcatgag aacgcccatc
      481 agaatcgggt aatcgcgcac cgtcagcgcg tcgtagaaga ggcggcccat gcctggccag
      541 gagaaaaccg tctcggtcag cacggcgccg gtgagataga gcggcacgtc gatcccgacc
      601 accgtcacga acgggatcag cgcgttgcgc agcacgtggc ccatgagcag gcgccgctcg
      661 gccagacctt tggcgcgagc ggtgcgcaca tagtcctcgc ccaacgcctc gatcgtcgag
      721 gagcggacgt agcggctcca cccggcgatg ctgccgatgg cgatggtgaa cgcgggcagc
      781 gccagatgtc gcaccagatc gccgggtccg ccgcctccgg tggccgagta cattccggcc
      841 gatggcagcc agccgaggcg gacggcgaac aggatctgca gcatcagccc gaaccagaag
      901 accggcatcg ccagcccgaa gtaactgacg aacgagacga tgttgtcgaa gaagccgtac
      961 ggccgccgcg cggccaggat gccgaaggtg agcgccgccg ccagagagat gagcatgccg
     1021 gtgcccatca gcaggagcgt gttgggcacg gtgtcccaga tcacctctga gaccgggcgg
     1081 tattgactgt aggagatgcc gagatttcct cgggccaggt tgcccagcca gatcaggagc
     1141 tgctcgtgca tgggccggtc cagccccatg ttgtgccgga tcgcctgcaa ctgctcttcg
     1201 ctcatgcggg cgttgcgcgc gaagagcgcg tcggggccgc ccggcgcgag ctgcataaag
     1261 atgaacgaca gcacggccac cccgagcaga agcggaatgg ctcccagcag gcgtcggatg
     1321 aggaagcggg tcatggcgcg aaccccggca cgccgggcac gggcggaggt gcccgtgccc
     1381 ggcattcatc ccgttacgtt acttggtgag ataccactcc tgcgcgttcc agaggaggtt
     1441 gttgaacgcg ctcggccaga acccgccgat ggtgttgtac accgccggga acgacttctc
     1501 ccaatagatg aaggtcactg gcacttcttc gtggagcagc tcctggatct gccagtaaat
     1561 cttctgccgc tcgtcctggt ccgtggtggt gtcgccggcc gtcgtcaact cgtcgatctt
     1621 gtcctggaag ctgtactggt gaaaataggc cggcaggttg ccgccggacc ccgtcggtgt
     1681 ctccgggatc tggctggagt gccagtagaa cgtgtcggtc gggtcattgc cattgaccca
     1741 agcgaagagg cacccggtca tcttctccgt gaactggtat ccctcgggtc cccagatggt
     1801 cgagatgtcc tggttgagca cggtggtctt gacgccgatg ctgttccagc tttgggcgac
     1861 aacctggctg atgcgctcgc tgtcgacgtt gccggagacg aaccacaact cgatttcgag
     1921 cggcttcacc tcgccgtcgc cgacgttcgg gtcgtccgtc ggcaccggac cctccagcac
     1981 gccgtccgcg ccgggggtca agccggcgtc ggccagcagt gccttggcct gctccagatc
     2041 gtaggggcgc ggctcgatgt tcgggttgaa cgcccaggac ccaggagcct ggtcggcaat
     2101 gc
//
LOCUS       DASSGX010000367         5174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_500366, whole genome shotgun sequence.
ACCESSION   DASSGX010000367 DASSGX010000000
VERSION     DASSGX010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5174
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500366"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VFI12_06525"
     CDS             complement(<1..267)
                     /locus_tag="VFI12_06525"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET7056099.1"
                     /translation="MDQEQAQMLMDYARAATSSAYVPYSQFPVGAAVMTESGAIFTGA
                     NIENASFPLTCCAERTAVYAAVNAGHKVITAVAVTAPNVVTVTPC"
     gene            300..929
                     /locus_tag="VFI12_06530"
     CDS             300..929
                     /locus_tag="VFI12_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056100.1"
                     /translation="MSDSWSPKIAAFVVQALDLALDPLDLAPEIVALKREPTSGISAV
                     ELQSSVGPTPFLVYHYLIGNGGPSQFDADVATLETAAALDTPGPRIVAHASAGDEAFI
                     LATTPSVQRSLTGQAEQKSTRPPMPIERARTRVPDRLIDKLREANRLAGDWLTAIRTA
                     GENGDDLSLTEQEAALALYLLDERSIQDLLQALNVLISTARNQAGEALG"
     gene            complement(937..1416)
                     /locus_tag="VFI12_06535"
     CDS             complement(937..1416)
                     /locus_tag="VFI12_06535"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET7056101.1"
                     /translation="MDLLDQMLEHDRWATAVLLDQSRTLSDAQLDQSFDIGHRTIRAT
                     FLHMIPNVEFWTRLMAGQIVDGEPEDASISELTQLHERSYDGFARQARRFRDDGRLND
                     AYVDHYQVRKSLGGTILMVIQHNAEHRTEIVHMLTRLGAPNVPEVDLGVWDYLQHNT"
     gene            complement(1435..1914)
                     /locus_tag="VFI12_06540"
     CDS             complement(1435..1914)
                     /locus_tag="VFI12_06540"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET7056102.1"
                     /translation="MASNTEAESVNRKLAVREDHWCFGCGRQNPIGLKLTFFEDSEQV
                     WAPWTPRREHQGYEGIVHGGLITTVLDEVMGWAIYVRKLWAVTGSMNIHFRKPVQIGE
                     PLTARAWVASVSGRKVDVRAELRRDSDGQLLADATALFIRVPEEQASAWQGRYIDQP"
     gene            2083..3507
                     /gene="lpdA"
                     /locus_tag="VFI12_06545"
     CDS             2083..3507
                     /gene="lpdA"
                     /locus_tag="VFI12_06545"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HET7056103.1"
                     /translation="MAEVAAPTHQFDLVVLGGGTGGYVAAIRAKQLGLNVAVVEELIL
                     GGCCLHRGCIPTKAYLKSADVYDDVKRATEFGVDIKGDISFNYNQALARSKGVVESQY
                     KGLLYLFDKKHKIPVFMGRGKLLNKSTVGVTPKDGGQPFSLQAKNIIINTGSRPRAIK
                     GIPYDGKTVINSDHAVVLEKLPKSFIVRGGGATGVEWASVYHRYGVPTTLVGNVVPQE
                     DEEIQQAMERALRRQKMDVVVGARPTADDIDITKNGVTMRLKDAKGKERTVEAETLLV
                     GIGREPVVEDIGLEELGIKTDKGGFIIADPMMRTNVEGIYAIGDVNGQQMLAHTAQHH
                     GIIAVEHICGLNPFPLDVLNSPSCTYAAPEIGSVGMTEKQARDAGINVKIGKFPMKPN
                     AKATIEGHTDGFTKMIADADTNDLLGIHIIGPHATEMIAEGGLARLLESTPYELAISV
                     HPHPTVSEVIGEAAHDLLGHAIHI"
     gene            complement(3887..4096)
                     /locus_tag="VFI12_06550"
     CDS             complement(3887..4096)
                     /locus_tag="VFI12_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056104.1"
                     /translation="MFRLNDLEIRAHALRHEENIARAHRASRLSWTWEIADESSCNKT
                     GWVSTLFSRMTTSAFQPRQPMQTCS"
     gene            complement(4332..>5174)
                     /locus_tag="VFI12_06555"
     CDS             complement(4332..>5174)
                     /locus_tag="VFI12_06555"
                     /inference="COORDINATES: protein motif:HMM:NF021493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadG family pilus assembly protein"
                     /protein_id="HET7056105.1"
                     /translation="VMKSKAVQSEVDTVVNQNKMNFGTVTSIQCEFINDASQPLGSCS
                     GAIPSSATGVRVTVTENHPTFFIKVVPGAPNNVTTSAVAAANIRVLPPLGGGPFLPCS
                     KQAIRTDNGETMDLVFLDDNYNFLEVNDEAVGVKFNIYGPNDKVVEDCGIAPDKYNGA
                     ADQSRPCVTAPGWCYFKEGSYTGPVNQAVDGIDGCQAGAVSPYNCVAFLPIATIKDQP
                     LPANADRVWVVAFLPFYIEQGAQPNKYYGTLLDDYAIMGLGQEGSGGWTPDYHGPVTI
                     RLTL"
BASE COUNT          959 a         1624 c         1612 g          979 t
ORIGIN      
        1 gcagggcgtg accgtcacca cgttcggcgc ggtcactgcc accgccgtga tcaccttgtg
       61 gccggcgttg accgcggcat agacggcggt ccgctcggcg cagcaggtga gcggaaagct
      121 ggcgttctcg atgttcgcgc cggtgaagat cgcgccgctt tcggtcatga ccgccgcccc
      181 aaccgggaat tgcgagtaag gcacgtaggc ggatgaggtc gccgcgcgag cgtagtccat
      241 gagcatctgc gcttgctcct gatccatcga ccgtcaccct ttcccggtat catcgcgcca
      301 tgtctgattc atggtcgccg aaaatagctg cattcgtcgt ccaggcgctc gatcttgcgc
      361 tcgatccgct cgatctggct ccggagattg tcgcactcaa gcgcgagccg acttccggca
      421 tcagtgccgt ggagttacag tcgagcgtcg gcccgacacc cttccttgtc tatcactacc
      481 tgatcggcaa cggcggcccg tcgcaattcg acgccgacgt cgcaacgctc gaaacggcgg
      541 ccgcactcga tacgccggga ccgaggatcg ttgcccatgc ctcggccggc gatgaggcgt
      601 tcatcctggc caccacgccg tcggtccagc gttcactgac cggccaggcc gagcagaagt
      661 cgacgcgacc gccgatgccg atcgaacgtg cccggacgcg ggtgccggac cggctgatcg
      721 acaagctgcg cgaggcgaac cggctggccg gggattggct gaccgcgatc cggaccgccg
      781 gcgagaacgg cgacgacctc agcctgaccg agcaagaggc ggcgctcgcg ctctacctcc
      841 tcgacgagcg ctcgattcag gacctcctcc aggcgctgaa cgtcctgatc tcaaccgccc
      901 gcaaccaggc cggagaggcc cttggttagc tgacgttcat gtgttgtgct gcaggtaatc
      961 ccagacgccg agatcgacct cgggcacgtt aggcgcgccg aggcgggtga gcatgtggac
     1021 gatctccgtg cggtgctcgg cattgtgctg gatgaccatc aggattgtgc cgccgagcga
     1081 cttccgcacc tggtagtgat cgacgtaggc atcgtttagc cgcccatcgt cgcgaaacct
     1141 gcgcgcctgc ctcgcgaaac cgtcgtacga tcgttcgtga agctgggtaa gttcggagat
     1201 cgaggcgtct tccggttcac cgtcaacgat ttggccggcc atcaatcggg tccagaactc
     1261 gacattgggg atcatgtgga gaaaggtggc acggatcgtc ctgtgcccga tgtcgaacga
     1321 ctggtcgagc tgcgcgtcgc tcagggtgcg tgactgatcg agaagcaccg cggtggccca
     1381 gcggtcgtgt tccagcattt ggtcaagcag atccattcat gcctcctgcg tgtgctatgg
     1441 ctgatcaatg tagcggcctt gccaggcgct ggcttgctcc tcggggacgc ggatgaagag
     1501 ggcggtggcg tcggcgagga gctgtccatc gctgtcgcgt ctgagctcgg cgcggacgtc
     1561 gaccttgcga ccggaaactg acgcaaccca cgccctggcg gtcaggggct cgccgatctg
     1621 gaccggcttg cggaaatgga tgttcatgct gcccgtaacc gcccaaagct tgcggacgta
     1681 gatggcccag cccatgacct cgtcgagcac cgtcgtaatc aatccgccat ggacgatccc
     1741 ctcgtagccc tgatgctcgc gccgtggcgt ccagggagcc cagacctgct cactatcctc
     1801 gaagaatgtc agcttgagtc cgatcggatt ctgacgcccg cagccgaagc accagtggtc
     1861 ctcgcggacc gccagcttgc gattgaccga ttccgcctct gtgttgctcg ccaagagcct
     1921 gcctcccgcc tcagatatca cgccgtcgag tctaccgccc gacgcgctcg caccgggagc
     1981 gccggcccgc caccgtatac ttccgggacg gagatgccgg gaatgcgccg gcgcacgggc
     2041 aggctacgtg cctgcgatgt tcacgaggag tccagcctga cgatggcgga agttgcggca
     2101 cctacccacc aattcgatct cgttgtgctc ggtggcggca ccggcggcta cgtggcggcg
     2161 atccgcgcca agcagctcgg attgaacgtc gcggtcgtcg aggagttgat tctcggcgga
     2221 tgctgcctgc accgcggttg cattccgacc aaggcgtacc tgaagtcggc cgacgtctac
     2281 gacgacgtca aacgggccac cgaattcggc gtcgacatca agggcgatat ctccttcaac
     2341 tataaccagg cgctggcacg gtcgaagggt gtcgtggaga gccagtacaa gggcctcctc
     2401 taccttttcg acaagaagca caagatcccg gtgttcatgg gccgcggcaa gctgctcaac
     2461 aagtcgaccg tcggcgtcac gccgaaggac ggcggccagc ccttctcgct ccaggcgaag
     2521 aacatcatca tcaacaccgg ctcccggccg cgcgcgatca aggggatccc gtacgatggc
     2581 aagaccgtga tcaactcgga tcacgcagtc gtcctcgaaa agttgccaaa gagcttcatc
     2641 gtccgcggcg gcggcgcgac cggcgtcgag tgggcctccg tctaccaccg ctacggcgtc
     2701 cccaccacgt tggtcggcaa cgtcgtccct caggaggacg aggagatcca gcaggcaatg
     2761 gagcgagcgc tccgccgcca gaagatggac gtcgtggtcg gcgcgcgccc gacggccgat
     2821 gacatcgaca tcaccaagaa cggcgtgacg atgcgcctga aggacgccaa gggcaaggag
     2881 cggaccgtcg aggccgagac attgctggtc gggatcggcc gcgagccagt ggtcgaggac
     2941 atcggtctcg aggaactcgg gatcaagacg gacaagggcg gcttcatcat cgctgacccc
     3001 atgatgcgga cgaacgttga ggggatttac gcgatcggcg atgtgaacgg ccagcagatg
     3061 ctcgcccaca ccgcgcagca ccacggcatc atcgccgtcg agcatatctg tgggctcaac
     3121 ccgttcccgc tcgatgtcct gaactcacca tcctgcacct atgccgcgcc cgagatcggt
     3181 tcggtcggca tgaccgagaa gcaggcccgg gatgccggca tcaatgtcaa gatcggcaag
     3241 tttccgatga agccgaacgc caaggcgacg atcgaggggc acaccgacgg cttcacgaag
     3301 atgattgccg atgccgacac caacgatctg ctcggcatcc atatcatcgg accgcacgcg
     3361 accgagatga tcgccgaggg tggtttggcg cggttactcg agagcacgcc gtacgagctg
     3421 gcaatctcgg ttcatccaca cccgaccgtc tccgaagtga tcggcgaggc cgcgcacgac
     3481 ctgctgggac acgcaatcca tatctagtcg tgagacgtgc gacgtgagtc gtgtgcaaga
     3541 aaacgggcga gggatttctc ctcgcccgtt tcatcgttca attgacatgt gcccgatctc
     3601 tgattatgaa gccgactcgc actgctcgac gttcctggat cgcagtggag ccgacaggaa
     3661 tgagccgatc gctccgatta gcgacgcggc gaagatgcgt actcgcctgg ggtttctggc
     3721 ttccgcgttt gcgcgtcgct tcttgaggtc agaccaaatc gggccgaacc gttcggcttc
     3781 gcgaatgtat cgcgtgtggt cgctcattgc ggcggcgtaa tccgcctctg aaatcctgat
     3841 catgttcgtt cccttttcaa ctcggagtca atgtgcggcg cgattcctag gagcaggtct
     3901 gcatcggctg ccggggctgg aaggccgatg tcgtcatgcg gctgaacagg gtcgagaccc
     3961 acccggtctt attgcagctc gactcgtcgg cgatctccca ggtccagctc aagcgggacg
     4021 cgcgatgcgc ccgggcgatg ttttcttcat gcctcagggc gtgggcgcga atttcgaggt
     4081 cgttcagacg aaacattgtg cggtttcctc ccgctcttgg acccttctgg tccatccttc
     4141 aactgcgctc ccttacgcca catcctgatc tttgacaaga gaaaacacat ccgtcaaagg
     4201 gcggagcttc cctcccaatt tcgacggatt ccaagcgcgg gtcgaggatc tccgcgagcg
     4261 gaactacgac agcgaacgct cggtggggcg agcgttcgct gtcaaccgct atggagttgt
     4321 gagggtgggg atcagagcgt caggcggatc gtgaccggac catgatagtc aggcgtccaa
     4381 ccgccgctcc cctcctggcc gagccccatg atcgcgtaat cgtcgagcag tgtgccgtag
     4441 tacttgttcg gctgagcacc ctgctcaatg tagaacggca ggaatgcgac cacccaaacc
     4501 cggtctgcgt ttgccgggag gggttggtct ttgatagtgg cgatcggcag gaatgccacg
     4561 cagttgtatg gagacaccgc gccggcctgg caaccgtcga tcccatccac tgcctggttg
     4621 accggcccgg tgtagctgcc ttccttgaaa taacaccacc ccggcgcggt cacgcacgga
     4681 cgcgactggt cggccgcccc gttgtacttg tctggagcga tgccacagtc ctcgacgacc
     4741 ttgtcattcg gaccgtagat gttgaacttg acgcccacgg cttcgtcgtt gacttcgagg
     4801 aagttgtagt tatcgtccag aaacacgagg tccatggtct cgccgttgtc cgtgcggata
     4861 gcttgcttcg agcagggcaa gaatggccct ccgccaagag gcgggaggac acggatgttg
     4921 gccgcggcga ctgccgaggt agtgacgtta ttcggcgcgc cgggcaccac cttgatgaag
     4981 aaggtcgggt gattttccgt gaccgtgacc cgcacacctg ttgcactcga cggaatggcg
     5041 cccgagcaac tccccagtgg ctggctcgcg tcattgatga actcgcactg aatcgatgtt
     5101 acggtgccga agttcatctt gttctggttg acgacggtgt cgacctcaga ctggactgcc
     5161 ttcgatttca tcac
//
LOCUS       DASSGX010000368         3533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1591108, whole genome shotgun sequence.
ACCESSION   DASSGX010000368 DASSGX010000000
VERSION     DASSGX010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1591108"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1169)
                     /locus_tag="VFI12_06560"
     CDS             complement(<1..1169)
                     /locus_tag="VFI12_06560"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HET7056106.1"
                     /translation="MDSVLKLLAVFALVAINGVFVAAEFALVGARPTRIDQLAAQGNR
                     WARLVKRAMDDPNRFISAAQVGITMASLGLGWLAEPAVAELLIPVLQPLLGEGGKIGA
                     HLVAGAIALFVITLLHIVLGEQVPKMIALQRAESTILTIAPVITAIAIPFRPLIALLY
                     WLTSVVLKALGLQWQGEHHLVYSEDELKMMITASHQKGILDENEEAMINRVFGFAEMM
                     TEQVMVPRTEIEAIPIRATLDEVVKAVADSGHSRYPVYGENLDDVRGVFHAKDLYSLL
                     ARNEQHRFSLRRMVREPLMVAESTNLDDLLSRMKRERTQIAIVVDEYGGTSGMVTLED
                     VMERIIGDVQDEFETAGEDVVVLEKGSAEVSGLLSLEDINDRFGQHFEDSYYNTLG"
     gene            complement(1277..2290)
                     /locus_tag="VFI12_06565"
     CDS             complement(1277..2290)
                     /locus_tag="VFI12_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7056107.1"
                     /translation="MSDSLQTFDVGKIDALVAETVGQDDKVDVPEEIQTQMAGDIEEP
                     EQDLERDLDVLQRLDPNFINDPVRLYLREIAETPLLTHQQEIDLAKRVEMDDQDATQL
                     FVRANLRLVVSIAKRYVNRGLTLLDLIQEGNIGLMRAVQKYDWRKGFRFSTYATWWIR
                     QAITRAIADQSRTIRLPVHMGDSISRYRKTLNTLAQELGRQPTPEEVAEAMAVAPEKI
                     HQIVQAAQRTISLETPIGSEDETSLGDLIADEVSETPYEAASESMLKRDVSAALDTLT
                     PREKLVLQLRFGLGHSHQHTLAEVGEQLQISRERVRQIENEALQKLRRLDGDRLFAYH
                     QEL"
     gene            complement(2780..3160)
                     /gene="erpA"
                     /locus_tag="VFI12_06570"
     CDS             complement(2780..3160)
                     /gene="erpA"
                     /locus_tag="VFI12_06570"
                     /inference="COORDINATES: protein motif:HMM:NF010147.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HET7056108.1"
                     /translation="MAATSTPMLSITEEAVNQLRSFLDEQGTPEHALRVFVAPGGCSG
                     LQYGMTIEESADEGDEVIETEGVRIIVDNFSAMYLGGATVDFVNSLMGGGFTIHNPNA
                     VASCSCGHSFDTGDDGQTSHGCSR"
     gene            complement(3279..>3533)
                     /locus_tag="VFI12_06575"
     CDS             complement(3279..>3533)
                     /locus_tag="VFI12_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056109.1"
                     /translation="PGLSAPSKVEEIWKDEFSYMYRDVPNGIYTLTMHPEIIGKGHRI
                     MLLERLVDWMADHEGVAFARLGDAADQWRGQQGGAPGTER"
BASE COUNT          627 a         1098 c         1115 g          693 t
ORIGIN      
        1 ccgagggtgt tgtagtacga gtcctcgaag tgctgtccga accgatcgtt gatgtcctcg
       61 agcgagagca ggccgctgac ttccgcggag cccttctcca gcacgacgac atcctcgccg
      121 gcggtctcga actcgtcctg gacgtcgccg atgatccgct ccatgacatc ttcgagcgtg
      181 accatgccgg acgtgccgcc gtactcatcg accacgatcg cgatctgcgt ccgctcgcgc
      241 ttcatccggc tcagcaagtc gtcgaggttg gtcgattcgg ccaccatcag cggctcgcgg
      301 accatgcggc gcaggctgaa gcggtgctgc tcgttccggg ccagcaggct gtacaagtcc
      361 ttggcgtgga agacgccgcg aacgtcgtcg aggttctctc cgtagaccgg gtagcgggaa
      421 tggccgctgt ccgccacggc cttgacgacc tcatcgagcg tggcccggat cgggattgct
      481 tcgatctcgg tgcgggggac catgacctgc tcggtcatca tctcggcgaa gccgaagacg
      541 cggttgatca tcgcctcctc gttctcatcg aggatgccct tctggtggct ggcggtgatc
      601 atcatcttga gttcgtcttc ggagtagacg agatgatgct ctccttgcca ctgcaggccc
      661 agggccttca ggacgactga tgtcagccag tacagcagcg cgatcagggg ccgaaatggg
      721 atcgcgattg ccgtgatcac cggtgcaatc gtgaggatgg tcgactcggc gcgctgcaac
      781 gcgatcatct tcgggacttg ctcgccgagc acgatgtgca ggagcgtgat cacgaacaag
      841 gcgatcgcgc cggcgacgag gtgggcgccg atcttcccgc cttcgccgag gagcggctgc
      901 aggaccggga tcagaagctc ggcgaccgcg ggctcggcca accaaccaag gccaagactg
      961 gccatcgtga tgccgacttg cgcggccgag atgaaccggt ttgggtcgtc catggctcgc
     1021 ttgacgagcc tggcccaccg gttgccttgc gccgcgagct gatcaatccg ggtcggtctg
     1081 gccccgacca gcgcgaactc ggcggcgacg aagacgccgt tgatcgccac gagggcaaag
     1141 acggcgagga gtttcagaac gctgtccatc gaattgccct agcgggcgcg catcctcccg
     1201 cgcacggcaa agcagcccag tcctcccgag gaggactggg ctgcaaggtt cgtaggttga
     1261 ctggggagat ccagtgctag agctcctggt ggtaggcaaa caggcggtca ccgtcgagtc
     1321 ggcgcagctt ctgcagcgcc tcgttctcga tctggcgcac gcgctcgcgg ctgatctgga
     1381 gctgttcccc aacctcggcc agcgtgtgct ggtgactgtg gcccaagccg aatcgtagct
     1441 gcaacaccag cttttcgcgc ggggtgagcg tgtccagcgc ggcgctgaca tcgcgcttga
     1501 gcatcgactc gctggcggcc tcgtacggag tctccgaaac ctcgtcggcg atcagatcgc
     1561 cgaggctcgt ttcgtcctcg ctgccgatcg gcgtctcgag cgagatggtc cgctgggcgg
     1621 cctgcacgat ctggtgaatc ttctccggcg ccaccgccat cgcctcggcg acctcttccg
     1681 gagtcggctg gcggccgagc tcctgggcga gggtgttgag ggtcttgcgg tagcgagaga
     1741 tcgaatcgcc catgtggacg gggagtcgga tcgtccggct ctggtccgca atcgcgcggg
     1801 tgatcgcctg gcgaatccac caggtcgcgt aggtgctgaa gcggaacccc ttgcgccagt
     1861 cgtacttctg gacggcgcgc atgaggccga tgttgccctc ctggatcagg tccagcaggg
     1921 tgagaccgcg attgacatac cgcttggcga tcgagaccac caggcggagg ttcgcccgaa
     1981 cgaagagctg cgttgcgtcc tggtcgtcca tttccacgcg cttggcaagg tcgatctcct
     2041 gctgatgagt cagcagtggt gtttcggcga tctcgcgcag gtagaggcgg acgggatcgt
     2101 tgatgaagtt cgggtcaagt cgctgaagga catccagatc gcgctcgaga tcctgttccg
     2161 gctcttctat gtcgccggcc atctgcgtct ggatttcttc ggggacatcg accttatcgt
     2221 cctggccgac ggtttcggca accagggcgt cgatcttccc aacgtcaaac gtctgcaacg
     2281 aatcactcat taagtgcagt actccttaca gggtcgttcc gtctcgaaat gacgggtggc
     2341 agttcatccg gcgatccgaa gcggtgacac ggcaagaacc caacggccga aaatgatgcg
     2401 cgcgagcgcg ttacgtaaag gtacgtcgac cgggcccgcg gggttcttgc tctgcaattc
     2461 ttcgcgacgt ctggtgcaac tgctcgcgat acggtcctat tggcgagaca gaatgagagg
     2521 cgttgtgagt caacagaccg tcccacaact tgtgaatatt atcacatatg ccccgatttg
     2581 ggaaggggtc gagctgtagt ttccgcaacg cacccacctc tgccgcgcct gtgaaaccgc
     2641 tcccacaacc gatcatatcg gatgtcgagc gaagcgcaat accctgtcgc gtgcaaagct
     2701 cacatcaggg gcggaaaata tgagggcgcg gccgatctgg ccgcgccctt gacgcagata
     2761 acctggttgt gggtcgcgcc tagcggctgc agccgtgcga ggtctgaccg tcgtcacccg
     2821 tgtcgaacga gtgcccgcag gagcaggagg cgaccgcatt cgggttgtgg atcgtgaatc
     2881 cgccacccat cagcgagttg acgaaatcga ccgtcgcgcc gcccaggtac atcgcgctga
     2941 agttgtcgac gatgatccgg acgccttcgg tttcgatgac ctcgtcaccc tcatcggcgg
     3001 attcctcgat cgtcatgccg tactggaggc cggagcaacc acccggcgcc acgaaaacgc
     3061 gcagcgcatg ctccggcgtg ccctgctcgt ccaggaacga cctgagctga ttgacggctt
     3121 cctcagtgat ggaaagcatt ggcgtgctgg tagcggccac ccgggactcc ttatgaatca
     3181 accttgcggt tcaaattacc tcaatgcggc ccgaaccgaa cgtccgggca atacttacgt
     3241 taggatagtt taccctatcc ggctgtcaag gagttacatc accgctccgt gccgggtgct
     3301 ccgccttgct ggccccgcca ctgatcggcg gcatcgccca ggcgggcgaa tgcgaccccc
     3361 tcgtggtcgg ccatccagtc gaccaaccgt tccagcaaca tgatccggtg ccccttgccg
     3421 atgatctccg ggtgcatggt cagggtgtag atgccgttcg gcacgtcccg gtacatgtag
     3481 gagaactcgt ccttccagat ctcctcgacc ttgctcggcg cggaaagacc cgg
//
LOCUS       DASSGX010000369         2836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1591460, whole genome shotgun sequence.
ACCESSION   DASSGX010000369 DASSGX010000000
VERSION     DASSGX010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1591460"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1332
                     /locus_tag="VFI12_06580"
     CDS             <1..1332
                     /locus_tag="VFI12_06580"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET7056110.1"
                     /translation="LALARLNIPGFLLYGGTIMPGEVNGQEATVRSVYEKIGAYKAGK
                     ITEEELKLFEDHACPTHGACGGQYTANTMAMAMEFIGLSPLGTASPPAVSDEQWHEGI
                     GTRGDVVNPRKMEIGRQAGKLIMEMLAKGIRPLDILTRDAFENAIAGVAATGGSTNSV
                     LHLLALAREAKVELTIDDFDEISRRTPLIADMMPGGKYAAADLDKAGGTPVVAKRMLE
                     GGYLKGDQMSPSGQTLSEEAAVAVETPGQDVVHTLDNPINEHGGLIILKGNIAPDGSV
                     IKLFGYERHYHKGPARVFDSEHEALEAVYGNKINPGDVVVIRYEGPRGGPGMQEMLAI
                     TAAIIGQGLGAECALVTDGRFSGATKGLMCGHVAPEAAVGGPIAAVHEGDTVVMDIDN
                     RRLDIEVSDDEIAKRLQSWRPQPARYTSGVMAKYASLVQQANEGAITKPLG"
     gene            complement(1459..2295)
                     /locus_tag="VFI12_06585"
     CDS             complement(1459..2295)
                     /locus_tag="VFI12_06585"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HET7056111.1"
                     /translation="MSTNSLTGNRTEVAVFVDLENIRYSTINSFGREPDPLVWRDKAL
                     NYGLMAVARAYADFDQHPAAIRMRLEVAGFEAQHFPAKRTTDSQGRERVVSRSDLNFA
                     VDIINTALARPDIETFLLFTGDKDFIRLVTTLRNRLGRRVVICGIPGSISPDLVTSAG
                     EEDQIILEPCIDNDVAVIRAIHTYIGQLHDGFVPTQSHMSRTLWRFLDKNALPSEHIE
                     AKIMEFVRKGILTKRQTVNGQNQELVTTELNWSNTLVIEALTDWTPEAPDDQFVDTQM
                     AK"
     gene            2519..>2836
                     /locus_tag="VFI12_06590"
     CDS             2519..>2836
                     /locus_tag="VFI12_06590"
                     /inference="COORDINATES: protein motif:HMM:NF013813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056112.1"
                     /translation="MKSIPFTKMSGSGNDFVVIDDRSGILGGLDLAAFTRTVSRHRVS
                     VGADGVIVLTDPPAERPALAYTWRYINADGSDGEMCGNGAMCAARFAVRAGIAEPHHR
                     FLTP"
BASE COUNT          523 a          879 c          943 g          491 t
ORIGIN      
        1 ctggcgctgg cccgcctcaa catccccggc ttcctgctct atggcggcac gatcatgccg
       61 ggcgaggtga acgggcagga agcgacggtc cgctcggtct atgagaagat cggcgcctac
      121 aaggccggca agatcaccga ggaggagctg aagctcttcg aggatcacgc ctgcccgacc
      181 cacggcgcgt gcggcggcca gtacaccgcg aacacgatgg cgatggcgat ggagttcatc
      241 ggcctctcgc ccctcggcac ggccagcccg ccggcggtca gcgacgagca gtggcacgag
      301 ggcatcggca ctcgcggcga cgtggtcaac ccgcgcaaga tggagatcgg ccggcaggcg
      361 ggcaagttga tcatggagat gctggcgaag ggcatccgtc cgctcgacat cctgacccgt
      421 gacgcattcg agaacgcaat tgccggcgtc gccgcgacgg gtggttcgac caattcggtc
      481 ctgcacctgc tggcgctcgc ccgcgaggcg aaggtcgagc tgacaatcga cgacttcgac
      541 gagatctcgc gccgcacgcc gctgatcgcc gacatgatgc cgggcggcaa gtacgccgcg
      601 gccgatctcg acaaggccgg cggtacgccg gtcgtcgcca agcggatgct cgaaggcggc
      661 tacctgaagg gcgaccagat gagcccgagc ggccagacgc tctcagagga agcagccgtg
      721 gcggtcgaga cgccggggca ggacgtggtc cacaccctcg ataacccgat caacgagcac
      781 ggcgggttga tcatcctcaa gggcaacatc gcgccggacg gctcggtgat caaactcttc
      841 ggctacgagc gccactatca caaggggccg gcccgggtct tcgattcgga gcatgaggcg
      901 ctcgaggcgg tctatggcaa caagatcaat ccgggtgatg tcgtcgtgat ccggtatgag
      961 gggccgaggg gtggcccggg catgcaggag atgctcgcga tcacggcggc gatcatcggg
     1021 caggggctcg gcgccgaatg cgcgctcgtc actgacggcc ggttcagcgg cgcgaccaag
     1081 ggcctgatgt gcggccacgt cgccccggaa gcggccgtgg gcggtccaat cgccgcggtt
     1141 cacgagggtg acacggtcgt catggacatc gacaaccgcc gcctcgacat cgaggtctcg
     1201 gacgacgaga tcgcgaagcg gctccagtcg tggcgtccgc aaccggcccg ctacacgagc
     1261 ggggtgatgg ccaagtacgc ctcgctggtc cagcaggcga atgaaggcgc gatcaccaag
     1321 ccacttgggt agtcgtgagg cgtgagactg gattgagtgg ggagcgtact tcgctcaatc
     1381 caggcatgaa ctgctcataa agtgagccgg accggaattc cggtccggct ctttctgttc
     1441 actggccact atcgtctgtc acttggccat ttgcgtgtcg acaaactggt cgtccggtgc
     1501 ttcgggtgtc cagtcggtga gggcctcgat gaccagggtg ttgctccagt tcagctcggt
     1561 cgtgaccagc tcctggttct gcccattgac cgtctggcgc ttggtcagga tgcccttgcg
     1621 gacgaattcc atgatcttgg cctcgatatg ctccgaaggc agcgcgttct tgtcgaggaa
     1681 gcgccaaagc gtccggctca tgtggctctg cgtgggcacg aagccgtcgt ggagctgacc
     1741 aatgtaggtg tggattgccc ggatcacggc gacgtcgttg tcgatgcatg gctcaaggat
     1801 gatctggtcc tcttcgccgg ccgacgtgac gagatcgggg ctgatcgagc cggggatgcc
     1861 gcagatcacc acgcgccgcc cgagccggtt gcgcagcgtc gtcaccagtc ggatgaaatc
     1921 cttgtcgccg gtgaagagca ggaaggtctc aatatccggg cgagcgaggg ccgtattgat
     1981 gatgtcgacc gcaaaattga ggtccgatcg ggagacaacg cgctcgcggc cctgactgtc
     2041 ggtggtgcgc ttggcgggga aatgctgcgc ctcgaacccg gctacttcga gccgcatccg
     2101 gatcgccgcg gggtgctggt cgaaatcggc ataggcccgg gcgacggcca tcaagccgta
     2161 attcagcgcc ttgtcccgcc agacaagcgg gtccggctcg cgcccaaatg agttgattgt
     2221 ggaataccgg atattctcga gatctacgaa aactgcgact tcggtgcggt ttcccgtcag
     2281 actattagtg ctcatagata cgtccgatcc gactctctta cgtcaaacga ccgcgcctca
     2341 ccgatcgcga atacgggcca gccatgtatc tcggagtgct tctccaccca aacctgcgcc
     2401 ttggcgagct ccgccgaaac gcatgcgcag cacgaaacgt gatctacgcc acattactgt
     2461 ctgatagtat cacgtcgccc gggaatgcag aatccccggg cggaagggca atttgcgcgt
     2521 gaaatcaatc ccgttcacga aaatgagcgg tagcggcaac gacttcgtgg tgatcgacga
     2581 ccggtcggga attctcggcg gtctcgatct cgccgcattc acgcggaccg tcagccgtca
     2641 ccgggtttcc gtcggcgcgg acggcgtgat cgtgcttacc gatccgccgg cagagcggcc
     2701 cgcgctggcc tatacgtggc gctacatcaa cgcggacggc agcgacggcg agatgtgcgg
     2761 caacggcgcg atgtgcgccg cccggttcgc cgttcgcgcc gggatcgccg agccgcacca
     2821 ccgcttcctg acgccg
//
LOCUS       DASSGX010000370         2303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_173796, whole genome shotgun sequence.
ACCESSION   DASSGX010000370 DASSGX010000000
VERSION     DASSGX010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173796"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VFI12_06595"
     CDS             <1..264
                     /locus_tag="VFI12_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056113.1"
                     /translation="QYWGGWKDGLVDLSPITDLVPAEIKTEAETMIESFKKGEQTIYT
                     VFTGPLKDQAGADKVADGTAMTDEELLGMTWFVEGVNGELPKS"
     gene            461..2062
                     /locus_tag="VFI12_06600"
     CDS             461..2062
                     /locus_tag="VFI12_06600"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7056114.1"
                     /translation="MKRDETMAVEATHHPVAIELIDVTKRFGPVVANQDISFDARYGE
                     VHALIGENGAGKSTLMSIVAGLYRPDSGRLRINGQPVLFKSPRDAIERGIGMVYQHFM
                     LVETMTVAENLLIGMPQPGIGLKTSAAATAVRDLSARFGLDVDPDAHIWQLSVGEQQR
                     VEIIRLLFRGAKILVLDEPTAVLTPAEAAGLIKTVRAMAAEGYCIIFISHKLDEVLQV
                     ADRITVLRRGQTVGTVTPAEVDRRALARMMVGREMAAAPIDRPAPGTGIALAVRNLCV
                     MSDRGLHAVNDLSLDVRNGEILGIAGVAGNGQREFAEAVTGLRPVVSGEIEVGGRDLS
                     GSSAAGMAAAGVAHIPEDRLATGLAGGIDVETNATLRNYKNAPLSKGPVLMRKAMGAF
                     ADRLIAEHDVKTPSRRTRARQLSGGNQQKLIIARELAGSPAVIIASYPTRGVDVGATE
                     AIHHLLRSHRDRGAAIILISEDLDELRSLSDRIGVLVRGRIIDTIPVEQAGNERLSML
                     MAGIGPDYAMSTPNGAPVESSAVDA"
     gene            2052..>2303
                     /locus_tag="VFI12_06605"
     CDS             2052..>2303
                     /locus_tag="VFI12_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056115.1"
                     /translation="MTHSRPQITLERIHHASPARWAGVTVSSILLALIACGIVLLISG
                     ENPFTVYRAMFEGAFGDRYALSETLVKMIPLLLAGLGVAI"
BASE COUNT          376 a          796 c          761 g          370 t
ORIGIN      
        1 cagtactggg gcggctggaa ggatggtctc gtcgaccttt caccgatcac cgacctcgtc
       61 ccggctgaga tcaagacgga ggctgagacg atgatcgagt ccttcaagaa gggcgagcag
      121 acgatctata ccgtcttcac cggcccgctc aaggatcagg ccggtgccga caaggtcgcc
      181 gacgggaccg ccatgaccga tgaggagctg ctcggcatga cctggttcgt cgagggcgtc
      241 aatggcgagc tcccgaagag ctagtgcgcg caggtgatga agcgtcgttt gatgtagcgc
      301 ggggcggccc gccccgctgt ggccgaaggc cacgatggtg cctttcgaaa gctgcttcgc
      361 gcatgcgaat cggtttcttg tcgacgaatg cggggcgagc cgccccgcgc tacgtttccc
      421 gggcgatgtt ctgaatgggt cgctgcttga ggctacgctc atgaagaggg acgagaccat
      481 ggccgtggag gcgacgcacc atccggtcgc gatcgagctg atcgacgtca ccaagcggtt
      541 cgggccggtc gtcgccaacc aggacatcag cttcgacgcg cgctacggcg aggtgcacgc
      601 gctgatcggc gagaacggcg cgggcaaatc cacgctgatg agcatcgtgg ccggcctcta
      661 tcgccccgat agcggccgcc tccggatcaa cggtcaaccg gtcctgttca agtccccgcg
      721 ggatgcgatc gagcgtggga tcggcatggt ctaccagcac tttatgctgg tcgagaccat
      781 gaccgtcgcc gagaacctgc tgatcgggat gccgcagccg ggcatcggcc tcaagactag
      841 cgccgccgcg accgccgtgc gcgatctctc ggcccgcttc ggcctcgatg tcgatccgga
      901 cgcccacatc tggcagctct cggtcggcga gcagcagcgc gtcgagatca tccgcctcct
      961 cttccggggc gcgaagatcc tcgttctcga cgagccgacc gccgtcctga ccccggccga
     1021 agccgccggg ctgatcaaga cggtccgggc gatggccgcc gagggctact gcatcatctt
     1081 tatctcgcac aagctggacg aggtgctcca ggtcgccgac cggatcactg tccttcgtcg
     1141 cggccagacc gtcggcacgg tcacgccggc cgaggtcgat cgtcgcgcac tggcccggat
     1201 gatggtcggc cgggaaatgg cagccgcgcc gatcgaccgg cccgcgcccg gcaccggaat
     1261 cgccctcgcg gtccgcaacc tctgtgtcat gagcgatcgc ggcttgcacg cggtcaacga
     1321 cctctcgctc gatgtccgca atggcgaaat ccttggcatc gcgggcgtgg ccggcaacgg
     1381 ccagcgcgag ttcgccgaag cagtcaccgg actgcgcccc gtggtcagcg gtgaaatcga
     1441 ggtcggcggc cgcgacctct ccggctcgtc agccgccggg atggccgccg ccggcgtcgc
     1501 ccacatcccg gaggaccgcc tcgcaaccgg gctggccggc gggatcgatg tcgagaccaa
     1561 tgccaccctg cgcaactaca agaacgcgcc gctctcgaag gggcccgtcc tgatgcgcaa
     1621 ggcgatgggc gccttcgccg atcgcctgat cgccgagcac gacgtcaaga cgcccagccg
     1681 ccgcacccgt gcccggcagc tctccggcgg caaccagcag aagctgatca tcgcgcggga
     1741 gctggccggc agtccggccg tgatcatcgc cagctacccg acccgcggcg tcgacgtcgg
     1801 cgcgaccgag gcgatccatc acctgctccg ctcgcatcgt gaccgcggcg cggcgatcat
     1861 cctcatctcg gaggacctcg acgagctgcg cagcctgtcg gaccggatcg gcgtgctggt
     1921 ccggggccgc attatcgaca cgattccggt cgagcaggcc ggcaacgagc ggctgagcat
     1981 gctgatggcc ggtattggac cggactacgc tatgtcgacg ccgaacggcg cgccggtcga
     2041 gagctccgct gttgacgcat agccggccgc agatcaccct cgagcgcatc caccacgcca
     2101 gcccggcgcg gtgggctggc gtgactgtca gctcgatcct gctcgccctg atcgcctgcg
     2161 gtatcgtcct gcttatctcc ggcgagaacc cgttcaccgt ctaccgcgcg atgttcgagg
     2221 gcgcgttcgg cgaccgctac gccctctcgg agacgctggt caaaatgatc ccgctcctgc
     2281 tggcggggct cggcgtcgcg atc
//
LOCUS       DASSGX010000371         4348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1155800, whole genome shotgun sequence.
ACCESSION   DASSGX010000371 DASSGX010000000
VERSION     DASSGX010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155800"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VFI12_06610"
     CDS             complement(<1..251)
                     /locus_tag="VFI12_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7056116.1"
                     /translation="MTPLIGRDIEVIDVEEALRRPGIRLVTITGPGGVGKTRLALHLA
                     QSLRTLFPDGLWYVPLDAIRTPDLVMPTLAQAVGVADDGS"
     gene            complement(393..2981)
                     /locus_tag="VFI12_06615"
     CDS             complement(393..2981)
                     /locus_tag="VFI12_06615"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7056117.1"
                     /translation="MTTSPPLPIPLTPLIGRDVDLIEIEELLRRPAVRLVTITGPGGV
                     GKTRLALHVAGRLEDAFPDGIWLVRLEAIREVELVLATIAQAVGIADSSASDLAGHIA
                     SWLGPGRHLFLIDNFEQVADASPVLIDLLQRCPGLNVLVTSRMSLGVSGEREYPLGPL
                     PLPEAVDGGHVGALLEYPAVRLFVDRASEVKPSFALTAENAADVLAICQRLDGLALAI
                     ELAAARIRVLTPAALRERLTSRLKLLTSGHRNLPARQQTMRDAIAWSYELLAEQDRPI
                     FRLLSAFAGGFGLDALEVVAAHLVATKLPDYADLDPDFLIDQMTDLVDHSLVRRIDAD
                     SGEPRYALFQTIQEFAEDQLAACGEAVAARRAQAEWVADFVERAEPELTGPNQAEWFA
                     RLGQEHDNIRAALSFAIEQQDAQLGQRIGGRLWRFWWIRGHMIEGHRWLEQILALPDE
                     ERGIARGHATFSMAALAEELGDWGAATAGYREALAAGQTFDDVLLQGHALNSLANVAH
                     MQGNYDESIRLNHEARACFEAAGYRRGVAGCMHNLATVEYLRGNLEGAEAGYRQSLAI
                     LEELQDERSRVMILANIGVIAYERRDFEAARDIQIQSVELTRAMGDEIGLCNALTNLA
                     GALRELGDIDQALTLNRQALEIADRLSLKRNVGYIRSGIGQILRDQGDNAGAARSFID
                     SLHDVRECGDQAATANVLGSLADLAHELGDSRLAARLFGAAKAIRDAIGTSHAPDQVD
                     DYERVLGLVRVAAGDDAFDMAWAAGMAAPLDDVVREAGCLVELASAARLDPRDVDLQQ
                     RTGLNPRELAIVRQFVAGKSNQEIADALGLNLGFVTTLIGQIYTRLGVDSRAGVTAFA
                     FKNGIV"
     gene            3109..>4348
                     /locus_tag="VFI12_06620"
     CDS             3109..>4348
                     /locus_tag="VFI12_06620"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HET7056118.1"
                     /translation="MRPWLDLFRARLRESADLPTLVHELEQIVREIAGSRTALLWAEP
                     EIAQHWSNPVSLPSFSPGDLLVTYCVAHPTVTYAERARIDSPALAALDAGLLIPLVTL
                     GEFVGLIVLDATEFTPDQIAALDEIAGLAAPALRAAQLAHRVDAAERSHATTAEELRV
                     AEIIQRSLLPESLPTIAGWRLDAFYKPARVVGGDLYDIIPLPGDLLGIVLGDASDKSV
                     PAALVMATVRTLLRASALRLVLPGQVLARVNDDLCAQIPPGMFVTCFFAILDTSTGRL
                     RFANAGQCAPYLCGPDGVTELRASGWPLGMLPGRTYDEGEITFAPGSTVVCFSDGLTE
                     THSPGGDMFGTDRITGTLAESERCAPLIPALLAAQQAFAGPSWDQEDDVTLISVTRMP
                     ESEPIQVASPAEPIVLASFTI"
BASE COUNT          708 a         1546 c         1337 g          757 t
ORIGIN      
        1 gacccgtcgt ccgcgacacc gaccgcctgg gcaagtgtcg gcatgaccag atccggggtc
       61 cggatcgcat ccagcgggac ataccagagc ccgtccggga acagggtccg cagcgattgc
      121 gccaaatgca gcgcgagccg cgtcttcccg actccccccg gccccgttat cgtcaccagc
      181 cggattcccg gacggcgcaa cgcctcctcg acatcgatca cttcgatgtc gcgcccgatc
      241 aatggtgtca tgggaatggg gacggtcatg tggtgctaag ggtttccagt ttctagtttc
      301 tcgtctttgg ttttccgttg tccgttttcg gttttccgtg tctatctacc aacgtccaac
      361 gtccatcaac ctctgctgct cgccttctcg actcacacta tcccattctt gaatgcgaag
      421 gccgtcacgc cggcgcggga atcaacgccg agcctggtgt agatctgccc gatcagggtg
      481 gtcacgaagc cgaggttcag gccgagggca tcggcgatct cctggtttga tttgcccgcc
      541 acgaactggc gcacgatcgc gagctcgcgc gggttcaatc cggtccgttg ctggaggtcg
      601 acatcgcgcg ggtcgagccg cgccgcgctc gcgagctcca ccaggcatcc ggcctcccgc
      661 acgacgtcgt cgagcggcgc agccatcccg gcggcccagg ccatgtcgaa agcgtcgtcc
      721 ccggccgcca cccggaccaa cccgagcacg cgctcgtaat catcgacctg gtccggcgcg
      781 tggctcgtgc cgatcgcgtc gcggatcgcc tttgccgcgc cgaacaaccg cgcggcgagc
      841 cgcgagtcgc ccagctcgtg ggccaaatcc gccagcgagc ccagcacgtt ggccgtcgcc
      901 gcctgatcgc cacactcccg cacatcgtga agcgaatcga tgaatgagcg ggccgctccg
      961 gcattgtctc cctgatcgcg caggatctgg ccgattccag agcggatata gcccacattc
     1021 cgcttgagac tgagccggtc ggcaatctcc agcgcctgcc gattgagcgt caatgcctgg
     1081 tcgatatcgc cgagctcacg gagcgcgccg gcgaggttcg tgagcgcgtt gcagagcccg
     1141 atctcgtcgc ccatcgcccg cgtcagctcg actgactgga tctggatgtc gcgcgctgct
     1201 tcgaaatccc gtcgctcata cgcgatcacg ccgatgttgg ccaggatcat cacccgggag
     1261 cgctcgtcct gaagctcctc gaggatcgcc agcgactgcc ggtacccggc ctccgccccc
     1321 tccaggtttc cacggagata ttcgactgtg gccaggttgt gcatgcagcc ggcgacaccc
     1381 cgccgatatc cggccgcttc aaagcacgcc cgcgcctcgt gattgagccg aatagactcg
     1441 tcgtaattgc cctgcatgtg agcgacgttg gccagggaat tgagcgcgtg cccctgcaag
     1501 agcacgtcgt cgaaggtctg gccggccgcc aacgcctccc ggtacccggc cgtcgccgct
     1561 ccccaatcgc ccagctcttc agccagggcg gccatcgaga aagtggcgtg gccgcgcgcg
     1621 atcccgcgct cctcgtcggg cagcgccagg atctgctcca gccaacgatg cccctcgatc
     1681 atgtgaccgc ggatccacca gaagcgccag agccggccac cgatccgctg cccgagctgg
     1741 gcatcctgct gctcgattgc gaatgacagt gccgcacgaa tgttgtcgtg ctcttgccca
     1801 agacgcgcga accattcggc ctggttcggc ccggtcagct ccggctcggc ccgttccacg
     1861 aagtcggcca cccactcggc ttgagcgcga cgagcggcga ctgcctcccc acacgcggcg
     1921 agttgatctt cggcgaattc ctggatcgtc tggaacaggg cgtagcgcgg ctcgcccgaa
     1981 tcggcgtcaa tacgccgaac caggctgtga tcgaccaggt cggtcatctg gtcaatcagg
     2041 aaatccgggt cgagatcggc atagtccggt aatttcgttg cgaccaggtg ggccgcgact
     2101 acctcgagcg cgtccagccc aaaaccgcca gcgaaggcgc tcagcagccg gaagatgggc
     2161 cgatcttgct ccgcgagcag ttcatagctc caggcaatcg cgtcgcgcat cgtctgctgc
     2221 cgcgccggca ggttgcgatg accactcgtc agcagtttga gccggctcgt cagccgctcc
     2281 cgcagcgcag ccggcgtcag gaccctgatc cgcgccgccg ccagctcgat cgccaacgcc
     2341 agcccgtcca accgctggca tatcgccagc acgtcggccg cattctcggc cgtcagggcg
     2401 aacgacggct tgacctcgct cgcccgatcg acgaacagcc gcaccgcggg atactccagt
     2461 aacgcgccga catgcccgcc atcgaccgcc tcgggcaagg gcagcggccc gagcggatac
     2521 tcgcgctcgc cggagacccc gagcgacatc cggctcgtga ccagcacgtt caacccgggg
     2581 cagcgttgga gcagatcgat cagcactggg ctggcgtcag ccacctgctc gaagttgtcg
     2641 atcaggaaga ggtgccggcc gggaccgagc caactcgcga tgtgcccggc cagatcactc
     2701 gccgagctat cggcaatccc gaccgcctgc gcgatcgtgg ccaggaccag ctcgacctcg
     2761 cggatcgcct ccagccggac cagccagatg ccgtccggga aggcgtcctc cagccgcccc
     2821 gccacgtgca gggcgagtcg agtcttgccg acgccgccgg gaccggtaat cgtcaccagg
     2881 cgcacggcgg gacggcgcag cagctcctcg atctcgatca ggtcgacatc gcgcccgatc
     2941 aacggcgtca ggggtatggg aaggggaggc gatgtagtca tatcatcagc ccatgcactg
     3001 ggctcagcct tttcgagata gatattggtt gcgccatatg aatatgcggt gtgccgatat
     3061 catacgtgat tatcaatcga tgctacctgt cggatcgccg tgtttgtgat gcgcccctgg
     3121 cttgacctct tccgcgcccg gctccgcgag tcggccgacc tccccacgct cgtccacgag
     3181 ctggagcaga tcgtgcgcga gatcgctgga tcccggacgg cgctgctctg ggccgaaccg
     3241 gagatcgcgc agcattggtc taatccggtc tcgcttccgt ccttctcgcc aggcgatctc
     3301 ttggtcacct actgtgttgc gcatccaact gtgacatacg ccgagcgcgc gcggatcgat
     3361 tcaccggccc tggccgcgct cgatgccggg ctgctgatcc cgctggtaac actcggcgaa
     3421 ttcgtcggcc tgatcgtcct cgacgctacc gaattcactc ccgaccagat cgccgcgctt
     3481 gacgagatcg ccggcctcgc cgcgcccgcg ctccgggccg cgcagctcgc gcaccgggtc
     3541 gatgcggccg agcgatccca cgcgaccacg gccgaggagc tgcgcgtcgc cgagatcatc
     3601 cagcgcagcc tgctgccgga atcgctgcca acgatcgccg gttggcggct cgatgccttc
     3661 tacaagccgg cccgggtcgt cgggggcgat ctttacgaca tcatcccgct ccccggcgac
     3721 ttgctcggaa tcgtcctcgg cgacgcgagc gacaagagcg tgccggccgc cctcgtgatg
     3781 gcgacggtcc ggaccctgct ccgggcatcc gcgctccgtc ttgtcctgcc cggccaggtg
     3841 ctggcccggg tcaatgacga tctttgcgcg cagattccgc ccgggatgtt cgtgacctgc
     3901 ttcttcgcga tcctcgacac cagcaccggg cggctccgct tcgcgaacgc cggtcagtgc
     3961 gcgccctacc tgtgcggtcc cgacggcgtt accgagctgc gggcgagcgg ctggccgctt
     4021 ggcatgttgc ccggcagaac ctacgatgag ggcgagatca ccttcgcgcc cggttcgacc
     4081 gtcgtctgct tcagcgacgg cctgactgag acgcacagcc ccggcggcga tatgttcgga
     4141 accgaccgca tcaccgggac gctggctgag tcggagcggt gcgctccgct gatcccggcg
     4201 ctgctggctg cccagcaggc attcgccggt ccgagctggg accaggaaga cgacgtgacg
     4261 ttgatcagcg tgacccgcat gccggaatct gaaccgattc aggttgcgtc cccggccgag
     4321 ccgatcgtgc tcgcgtcatt cacaatcc
//
LOCUS       DASSGX010000372         4390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1484225, whole genome shotgun sequence.
ACCESSION   DASSGX010000372 DASSGX010000000
VERSION     DASSGX010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1484225"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..1208)
                     /locus_tag="VFI12_06625"
     CDS             complement(3..1208)
                     /locus_tag="VFI12_06625"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7056119.1"
                     /translation="MTSLTREPASLSYRETSAGLPSASVIVVGYNSREYLDRCFLSLL
                     RVEYPGKFDILFVDNRSEDNSADYVRLAYPTVRVVDANENLGYAGGNNLGARRSSGDV
                     LVFLNPDTEVEPGWLAALVSPMVADSGVGLTTSRILMMHDRGRINTCGNEVSLAGITW
                     CRGAGQPAQDFDLDADVSAVSGCSFAIRRSLYTDLEGFDERFFMYLEDTDLSWRARAL
                     GYRCRYVANSIVYHDYSLSLSPRKVELLERNRYRMLGKHLSLRSVLALTPALLSTEVL
                     TWGYSTLRGPRHLLAKARATGWAVTQVGPVVRKRWRPSEVAILRAHDPSPPVVDGIGG
                     APSRIAQRALGAFARRTAEISLRFLPDPDQTDSPAFPSAKVIPEDAVVSQAGAPDFDS
                     SADSAGEPS"
     gene            1437..1676
                     /locus_tag="VFI12_06630"
     CDS             1437..1676
                     /locus_tag="VFI12_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056120.1"
                     /translation="MTTTVTTTTIASESIIGVGAVIALVVVLLLIGFLIAKEIMSSVL
                     DDRAPAVGRILNVGAIPLVLVFSFIIVTKLLPIFG"
     gene            1877..2770
                     /locus_tag="VFI12_06635"
     CDS             1877..2770
                     /locus_tag="VFI12_06635"
                     /inference="COORDINATES: protein motif:HMM:NF019380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1616 domain-containing protein"
                     /protein_id="HET7056121.1"
                     /translation="MDATLAERIRLGHLPYDLIVAFLLTLASAAASLLEAPPFVRIPL
                     GILLVLALPGFALVCMLFPSVDGPDGVARVTLSVALSLAAIPIVALVIDRSPWRIERV
                     TVSIALALVSAIALIVAAGLRARLYSDERYRAEITLPALPHPRAWTRDQFALVAAFAL
                     AIALFVYGGFDAAVTRISGEPTTEFALYNADGQAQFYPRDVTIGQATEIQISIANHEG
                     KRVRYEIVVGGAGKAVTVLPDLTLEAGKTWQGPVRFTVTQAGDQLPVRFELYRVDDQA
                     DSMPYRTLELLVTGHQPGERP"
     gene            2875..3732
                     /locus_tag="VFI12_06640"
     CDS             2875..3732
                     /locus_tag="VFI12_06640"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET7056122.1"
                     /translation="MVPAAYTLPLSEIGPALIGPRALLYLVGLMLAEIVTVLVDSRWG
                     VVGHAVILAFLVAQSGISAFPVGNDAASAPRSVERLRANFLVSLALVPLIRIVSLAMP
                     LTQFPEWTWYGLIAVPLLAAAWAAARACGYTRAEIGLSLDLKPAALLTTSVVGAAGVG
                     LGYIEYQILHPESLVETLSPVKVAAVTVTLFIGTGLTEELIFRGVLQVAGAELFGTMT
                     GIIYSAATFALLHIGHRSAVNVGYVFIVGIAFGLIARYTRSLAGVTIAHGVTNTCLFV
                     LFPHLLGGG"
     gene            3900..>4390
                     /locus_tag="VFI12_06645"
     CDS             3900..>4390
                     /locus_tag="VFI12_06645"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HET7056123.1"
                     /translation="MLAGGKGSRLRPLTATGAKQLVPVANKPVLFYSIEQLVEAGITE
                     IGIITGDTGEQVKAAVGDGSRFGATITYLPQSAPLGLAHAIITAREWLGDDPFCMFLG
                     DNFLRRGIGPFAEGFRANACNAQILLKPVVDPSAFGVAVLNEAGRVECLVEKPKDPIS
                     NLAV"
BASE COUNT          826 a         1311 c         1312 g          941 t
ORIGIN      
        1 catcaggagg gttctccggc actgtccgca gacgagtcga aatcgggtgc gcccgcctgg
       61 ctcaccaccg cgtcctcggg gatcaccttg gcagatggaa aagcgggact gtctgtctga
      121 tcggggtccg gcaggaatcg caggctaatt tccgcggtgc ggcgggcaaa ggcgccgagt
      181 gcccgctggg caatcctcga cggcgctccg ccgatcccgt ccacaacagg aggggacgga
      241 tcgtgggctc gcaagatggc gacttccgat gggcgccacc gcttgcgcac gaccggcccg
      301 acctgcgtga cagcccagcc tgttgcgcgc gctttcgcca gcaagtgcct tggcccacgc
      361 aaggtggaat atccccaggt cagcacttcg gtgctgagca gtgccggtgt cagtgccaaa
      421 acgctcctga gcgaaagatg tttgcccagc atgcgatagc gattccgctc caggagctct
      481 acctttctcg ggctgagcga gagggagtag tcgtgataca cgatcgaatt ggcaacatat
      541 cggcatcgat atcccaacgc tctcgcacgc caggagaggt cggtgtcctc caggtacatg
      601 aagaatcgct cgtcgaaccc ctccaaatcg gtgtatagcg agcgcctgat tgcgaatgag
      661 cagccggaca cggcagatac gtctgcgtcg aggtcaaaat cctgcgctgg ttgaccagct
      721 ccgcgacacc aggtgatgcc tgcaagcgag acctcattgc cgcacgtatt gattcggcca
      781 cgatcatgca tcatcaggat tcgggacgtc gtaagcccaa ctccactgtc ggcgaccatc
      841 ggcgacacga gagccgccaa ccaacccggc tctacttccg tatccggatt gaggaagaca
      901 agcacgtcgc cagacgagcg ccgggcaccc agattgttcc cgcccgcata cccgaggttc
      961 tcgtttgcat ccacaacgcg cacggtgggg taggccagtc ggacatagtc cgcagaattg
     1021 tcctctgatc gattgtcaac aaacaggatg tcgaacttgc ccggatattc cacgcgcagg
     1081 agggacaaga agcatcggtc cagatattcc cggctgttgt atccaacgac aatgacgctt
     1141 gcgctgggga gtccggctga agtttcccgg tacgacaggg aagccggttc gcgggtcagc
     1201 gaggtcatct acgagcgggt cctgtctata ggcggtttgg ggggcggcgt tgtctgtcgc
     1261 aaatcgcacc ataccagccg ggagacgttt gggctagccc gttcttctac aaacggaaga
     1321 attgggacct ttgcacttcc agtcgcgctc tctcagcaaa acgtcaattc ggctcgaatg
     1381 tgaccgtgtt gtacgttcaa ttgacgggca cgaggccgtg tgtgatgatc tggtgcatga
     1441 cgactacggt gaccacaacc acgattgcca gtgagtccat catcggtgtg ggcgcagtca
     1501 ttgcgctcgt ggttgtgctg ctcctgatcg gattcctgat cgccaaagag atcatgtcat
     1561 cggtgctgga cgatcgggcc cccgccgttg gccgaatcct gaacgtcgga gcaatcccgc
     1621 tggtgctggt cttcagcttc atcatcgtga cgaaactgct gccaatcttc ggctagtagt
     1681 tccatttcac ctaagatata tctcgacggc caacccaagg tgttgtacat caacaactta
     1741 ttgcggcgca cgctttgttc ctgtgcaggc gcaatcaact gtattgccgt cgagagcatg
     1801 ggcgtggatg actgagcgca ccgagcttgt acatcccaaa tcgggatcga tacccgctac
     1861 gcacactgaa tctgacgtgg acgcaactct tgctgagcgc atccggttgg gtcatcttcc
     1921 gtacgacctg atcgtggcat tcctcctcac gctggccagc gcggccgcct cgttgctcga
     1981 agcgcctccg ttcgtgcgaa tcccgttagg catactgctg gttctcgcct tgcccggatt
     2041 tgcgctcgta tgcatgctct ttccaagtgt cgacggaccg gatggcgttg cccgggtcac
     2101 actgagtgtc gcgctatcgc tcgccgcaat cccgatcgtg gcgctggtta tcgaccgctc
     2161 accatggcgc atcgagcgcg tgacggtaag cattgctctt gcgctggttt cagcaatagc
     2221 cctcatcgtt gcggcgggct tgcgcgctcg tctttattct gacgaacgct atagggccga
     2281 gatcaccctt cctgcgctac ctcatccacg tgcatggact cgcgaccaat ttgcgctagt
     2341 ggcggcattt gcactcgcca tcgcactctt cgtctacggt ggcttcgatg cggcggttac
     2401 ccggatctcc ggcgagccga ccactgagtt cgcgctctac aacgcggacg gccaggccca
     2461 gttctatccg cgtgacgtga ctataggcca agcgacggaa attcaaatca gcatcgccaa
     2521 ccacgaggga aagcgcgttc gctacgagat cgtcgtcgga ggcgcaggca aggcggttac
     2581 cgtgctgccc gatctgacgc tcgaagctgg gaagacctgg cagggaccgg ttcgtttcac
     2641 ggtcacccag gcgggcgacc agttgccggt gcgttttgag ctttaccgcg ttgatgacca
     2701 ggcagactcc atgccctatc gcaccctcga attattggtg accggccacc agccgggtga
     2761 gcgtccgtga aagtggcgat gaagcggcgc ggaccgcgat cacggcatcc ggtccgtact
     2821 cccggccgcc tcgtatctcg gagggtaccg gacggaccgg atcgtaagcg gttggtggtg
     2881 ccggctgcct atacgttgcc gctgtcggag atcggaccag cactgatcgg acctcgagcg
     2941 cttctctatc tggttggcct gatgcttgcc gaaatcgtga ccgtgctggt cgattcccgc
     3001 tggggtgtag tcggccatgc ggtcattctt gcgttccttg tggcccaatc cggaatcagc
     3061 gcgttccccg tcggaaatga cgcggcatcg gcgcctcgat cggttgagcg tctccgtgcc
     3121 aatttccttg tctcgcttgc tttggtgccc ttgattcgga tcgtttcgtt ggcgatgccg
     3181 ttgacccagt tcccggagtg gacatggtac ggactaattg ccgtgccact cctcgccgcg
     3241 gcctgggccg ccgcccgcgc ttgtggatat acccgcgcag agataggact cagtctcgac
     3301 ctgaaaccgg ccgcgctcct gaccacgagc gtggtgggtg cagccggagt cggcctcggc
     3361 tatatcgagt accagatctt gcatcccgag tcgctcgtcg aaacactctc cccggtcaag
     3421 gtggccgcag ttacggtcac gcttttcatc ggtaccgggc tcaccgagga gctgatattc
     3481 cgcggcgtcc tccaggttgc cggcgctgaa ttgtttggca cgatgaccgg catcatttac
     3541 tccgctgcca ccttcgcctt gttgcacatc ggtcaccgct cggcagtgaa cgttggctat
     3601 gtgtttatcg tcggcattgc ttttgggctg attgcacggt acacgcgatc actcgcggga
     3661 gtgacgatcg ctcatggtgt gacgaacaca tgcctgttcg tgctttttcc gcacctgctt
     3721 ggaggaggat gacctctgaa catgggcgaa tgattacggc attcgaaagc gaatcctgag
     3781 atcgtcggct atgctggcta ccaggtctca acgcgattaa ttgatgacgt gccggacgga
     3841 cgtcgagaat gatgatggag ccactgatgg gcgataggcg accgtctttg aagggactcg
     3901 tgctggccgg aggcaagggc tcgcggttgc gaccactgac tgcaaccggg gcaaagcaac
     3961 tggtgcccgt cgcgaacaag cccgtcttgt tctatagcat tgaacagctc gtcgaggcag
     4021 gaatcaccga aatcggaatt attaccggcg acaccggtga gcaggtgaag gccgccgtag
     4081 gcgatggatc gcgctttggc gccactatca cctacctccc gcaatcggcg ccgctcggac
     4141 tggcgcatgc gatcatcacc gctcgcgagt ggctcggaga cgatcccttc tgcatgttcc
     4201 tcggtgacaa ctttctgaga cgaggaattg gtccatttgc cgaaggattc cgggcaaacg
     4261 cctgcaacgc gcaaattctc ctgaagccgg tggttgaccc gtcggcgttc ggagtcgccg
     4321 tcctgaacga agcgggacgg gtggagtgtt tggtcgagaa gccaaaggat cccatctcaa
     4381 atctcgcggt
//
LOCUS       DASSGX010000373         3438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_67598, whole genome shotgun sequence.
ACCESSION   DASSGX010000373 DASSGX010000000
VERSION     DASSGX010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3438
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67598"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VFI12_06650"
     CDS             <1..510
                     /locus_tag="VFI12_06650"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7056124.1"
                     /translation="PYIDPAVAEAMGVDLVDLDTLLRESDFVVVSAALTPETRNMIDA
                     DRLALMKPTAFLISTARGGLVDQQALYEALRDHRIAGAGMDVFAQEPVDPNEPILQLD
                     NVVVTPHALCWTDECERMMGENVLGSIQDVAAGRVPTNVVNRDAAETPTMQEKLRRYR
                     ERTEGSGAS"
     gene            507..1286
                     /locus_tag="VFI12_06655"
     CDS             507..1286
                     /locus_tag="VFI12_06655"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HET7056125.1"
                     /translation="MRENRVKRALQNGERSLGTMVFEFPTTGIGRIIAEAGAEFVIYD
                     MEHTGWSIETIRGLMASTRAADLVPMVRVPATEYHLIARPLDVGAMGLMIPMVETEAQ
                     ARKIVDSAKYPPVGHRGAAFGVAHDDYTGGDILAKMASANEELLLIAQIETGTGVENA
                     DAIAGVEGIDCLWIGHFDLTNSLGIPGQFTHPTYLEAVDRVLAACDRHGKTPGIMAGS
                     VADGEVELQQGFRALAYSGDLWLYQQALRSGLDALRAVPNA"
     gene            1386..2972
                     /locus_tag="VFI12_06660"
     CDS             1386..2972
                     /locus_tag="VFI12_06660"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET7056126.1"
                     /translation="MDRKLSIDPKHRDAIEAFARSRTSRRQVLRGAAGLAALGVAGRT
                     RVAAARSAQPSLTPAAISAAQRALRRQSADSAQAAVDAANALDPKPAELNVVYEDSLQ
                     TQDPINFSGPMWEELTGIKINTVAKPFPELFAAQVAEHLGATGAYDVLSFPPAWTADF
                     VAQGLPEPLDPYIEQYMAPEDLDDFHPLYKALMNYGGQKYGIFDDGDVIILYYRTDLF
                     GDAANQDDFKAQYGYDLAAPKDWQQYDDIQAFFTEQGGGNSWGGASQRAPGQVYGWFS
                     EEFRNRGGRFFDDETLDAALDSQAGIDTLSRMIASNKTMPPGVETWGFVEVLTGWMAG
                     QLAMIGGTWPPIGRWSEGTTVEQLNWVPETQVQGKVGYSIMPMGHSLHNSGFSLGVSS
                     DSANKEAAYLFIQWMTSPSISLQRVMLPYALRDPYRLSHFSSPEYRGRWATAGDYLDT
                     LQAAADTALLDIIMPGSSEYHTALDQLVTSAQAGTSVEEALATGNAAFNEITDRIGRD
                     SQKVAYETFKALKGSYYEGS"
     gene            3156..>3438
                     /locus_tag="VFI12_06665"
     CDS             3156..>3438
                     /locus_tag="VFI12_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056127.1"
                     /translation="MLPALLLILGFGVVPLIQSLVLSFQRWDLQSQAHPFIGFENYRA
                     ALSDSRVWGALQNTGIIVVAATGLEFLLGLGLALLFSGDFKGKRLAMPVL"
BASE COUNT          593 a         1122 c         1177 g          546 t
ORIGIN      
        1 ccctacatcg atccggccgt ggcggaggcg atgggggtcg atctcgtcga tctcgacacc
       61 ctcctgcgcg agtccgattt cgtcgtcgtc tctgccgcgt tgacaccgga gacgcgcaat
      121 atgatcgacg ccgaccgcct cgccctgatg aaaccgaccg cttttctgat cagcaccgcc
      181 cgcggcgggc tggtcgatca gcaggcgctc tacgaggcgc tgcgcgacca tcgcatcgcg
      241 ggtgcgggga tggacgtttt cgcgcaggag ccggtcgatc ccaacgagcc gattctgcag
      301 ctcgacaacg tcgtcgtcac gccgcacgcg ctctgctgga cggacgagtg cgagcggatg
      361 atgggcgaga acgttttggg ctcgatccag gacgtcgccg ccgggcgcgt gccaacgaac
      421 gtggtcaacc gcgacgcggc cgagaccccg acgatgcagg agaaattgcg gcggtatcgc
      481 gagcggacgg agggatcagg ggcatcatga gggagaaccg ggtcaagcgt gctttgcaga
      541 acggcgagcg gtcgctggga acgatggtct tcgagttccc gacgactggt atcggccgca
      601 tcatcgccga agccggggcc gagttcgtca tctacgacat ggagcacacc ggctggagca
      661 tcgagacgat ccgcgggctg atggccagca cgcgagcggc ggatctggtg ccgatggtgc
      721 gcgtaccggc gaccgagtac cacctgatcg cgcgcccgct cgacgtcggc gccatgggct
      781 tgatgatccc gatggtcgag accgaggccc aggcgcgcaa aatcgtcgat tcggccaagt
      841 acccgccggt cgggcatcgc ggtgcggcct tcggcgtcgc ccacgacgac tacaccggcg
      901 gcgacatcct ggccaagatg gcttcggcca acgaagagct gctcctcatc gcccagatcg
      961 aaaccgggac cggcgtcgag aacgcggatg cgatcgcggg tgtcgagggg atcgactgcc
     1021 tttggatcgg gcacttcgat ctgaccaatt cgctcggcat ccccggccag ttcacccacc
     1081 cgacgtacct ggaggcggtc gaccgggtgc tggcggcctg cgatcgccac gggaagacac
     1141 cggggatcat ggcgggttcg gtggccgacg gcgaggtgga attgcaacag gggttccggg
     1201 cgctggcgta ttccggtgat ctctggctct atcagcaggc actgcggtcg gggctggacg
     1261 ccctgcgcgc ggtgcccaac gcctaggccg agcggagcgc ctcggctggc acctccgccg
     1321 cgcgcgggcg cggcggcccg ggaatggccc acgcggccgt gggccggagt cgaaaggcga
     1381 cgcgcatgga tcggaaactg tcgatcgacc ccaagcatcg tgacgccatc gaggcatttg
     1441 cccgctctcg gacatcgcgc cggcaggtgc tgcgcggcgc ggcaggcctg gccgcgctcg
     1501 gagtcgccgg gcgaacgcgg gtcgccgcgg cccgcagcgc gcaaccgagc ctgaccccgg
     1561 cggcgatcag cgcggcgcag cgcgctctgc gccggcagtc ggccgactcg gcgcaagcgg
     1621 ccgtcgacgc ggcgaacgcg ctcgatccga agccggccga acttaacgtc gtctacgagg
     1681 attcgctgca gactcaggat ccgatcaatt tctccggacc gatgtgggaa gagttgaccg
     1741 ggatcaaaat caacaccgtc gccaaaccct tcccggagct gttcgcggcg caagtcgcgg
     1801 agcatctcgg cgcgaccggc gcctacgacg tgctgagctt ccccccggcc tggacggccg
     1861 atttcgtcgc ccaagggctg cccgagccgc tcgatcccta catcgagcag tacatggcgc
     1921 cggaagatct cgacgatttc cacccgctct acaaggcgct gatgaactac ggcgggcaaa
     1981 agtacgggat cttcgacgac ggcgacgtca tcatcctcta ctaccgcacc gatctcttcg
     2041 gcgacgcggc caaccaggac gacttcaagg cacagtacgg ctatgacctg gcggcgccga
     2101 aggactggca gcagtacgac gatatccagg ccttcttcac cgagcagggc ggcggcaata
     2161 gctggggcgg cgccagccag cgcgcgccgg gccaggtcta cggttggttc tcggaagagt
     2221 tccgcaaccg gggcggccgc ttctttgacg acgagacgct cgacgccgcg ctcgacagcc
     2281 aggccggcat cgacacgctc tctcgcatga tcgcctccaa caagacgatg ccgcccggcg
     2341 tcgagacgtg gggtttcgtg gaagtgctca cgggctggat ggcggggcaa ctcgcgatga
     2401 tcggcggcac ctggccgccg atcggtcggt ggtcggaggg aaccacggtc gagcagctca
     2461 actgggtacc ggagacgcag gtgcagggca aggtcggcta ctcgatcatg ccgatgggcc
     2521 acagcctgca caactccggg ttctccctcg gcgtctcctc cgacagcgcg aacaaagagg
     2581 cggcatacct cttcatccag tggatgacga gcccatcgat cagcctgcag cgggtgatgc
     2641 tcccctacgc gttgcgcgac ccgtaccggc tgagccactt ctcctcgccc gagtaccgtg
     2701 gccgctgggc gacggccggc gattacctcg acacgctgca agcggcggcg gacaccgcgc
     2761 tgctcgacat catcatgccg ggctcctccg agtaccacac cgcgctcgat caactcgtga
     2821 cgtcggccca ggctggcacg tcagtcgaag aggcgctggc gacgggcaac gccgctttca
     2881 acgagatcac ggaccgtatc ggccgcgaca gccagaaggt ggcctacgag acgttcaagg
     2941 cgttgaaggg gtcgtactac gaagggtcat agggtgttgg gggggtaggg tgatcgggtg
     3001 atcgggtgat agggagccga gagtcggaat tcgacagccg cggggtagat ggagggacgc
     3061 tgccctcgtc gcaggctgcc gcccacccat caccctatca ccctgtcacc ctgtcaccct
     3121 acgtaaggcg ccggcgcatc gagggctggc tgctcgtgct gccggcgctg ctgctcatcc
     3181 tcgggttcgg cgtcgtcccg ctgatccagt cattggtcct cagcttccag cgctgggatc
     3241 tccagagcca agcgcatccc ttcatcgggt tcgagaacta ccgcgcggcg ctcagcgatt
     3301 cgcgcgtgtg gggcgcgctg cagaacacgg gaatcatcgt ggtcgcggcg acggggctgg
     3361 aattcctgct cggtttggga ctggcgctgc tgttctcagg ggacttcaag gggaagcggc
     3421 tggcgatgcc ggtgctga
//
LOCUS       DASSGX010000374         3833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1484360, whole genome shotgun sequence.
ACCESSION   DASSGX010000374 DASSGX010000000
VERSION     DASSGX010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1484360"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VFI12_06670"
     CDS             <1..409
                     /locus_tag="VFI12_06670"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HET7056128.1"
                     /translation="SYPVAGMVRDWRPEGYAVDHAIVPHGISVILNAPAVFRFTAPAA
                     PHRHLCAAQMLGADITDAGPQDAGEVLANRLIEIMRMTGMPSGLAEVGYSEADLDRLV
                     EGTLPQHRVTKLSPRPASADDLRGIFHNALRYW"
     gene            461..1219
                     /locus_tag="VFI12_06675"
     CDS             461..1219
                     /locus_tag="VFI12_06675"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7056129.1"
                     /translation="MTQLRDEPERTLSLNEMADIAALSPFHAVRVFRSITGYTPAAMQ
                     TAVRVQEAKRLLVAEGSSVTDACYAVGFSSLGSFSQRFATLTGVNPSEYRTAVDRADA
                     MADDLARLERRPLLRMHERHTVSGRIVGDTERAAFYFVGIFPPGPPQGRPLRGDVLDE
                     PGSFVIRHVPDGVYSIYCAAVAHPQHSIDWVMPDTEWQTGGGQKVRMICGVPVHSLEI
                     EMRHRRPVVVPLMTTVMAIPNLADDARLNHTLLA"
     gene            complement(1231..2052)
                     /gene="murI"
                     /locus_tag="VFI12_06680"
     CDS             complement(1231..2052)
                     /gene="murI"
                     /locus_tag="VFI12_06680"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HET7056130.1"
                     /translation="MNVHPSAPIGVFDSGLGGLSVVEQLRIELPRESILYAADSRFCP
                     YGQRSHDEIQERSRAMTRYLVAHGAKLVIVACNTASAAAIELLRAEFMVPIVALEPAV
                     KPAIALTRTGRIVVLATPSTAASPRLRGLIGRYGAGFDIRPIGVPGLADRIEAGDVDG
                     PAVHALLADRVQSQVADGVDVIVLGCTHYPFVRPVIHELAGPAVRIVDSGNAVARRAR
                     EVLMRAGLLAGIAAIPELRIHTSGEPDLVGPIVERMIGGRVPVGLIADDRVRMTA"
     gene            complement(2101..2472)
                     /locus_tag="VFI12_06685"
     CDS             complement(2101..2472)
                     /locus_tag="VFI12_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056131.1"
                     /translation="MDHAAFARPSREHPISPSAGNDGGVALDRALHLLRPAPQPFAWQ
                     NGNQLLLLDIEEAQWLVAELAFDPNGCIYTEVRRAAYQSQREAIGVLLSRALARGESA
                     LIDTVEQLDRYMTRHYAVTLI"
     gene            2671..3513
                     /locus_tag="VFI12_06690"
     CDS             2671..3513
                     /locus_tag="VFI12_06690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HET7056132.1"
                     /translation="MPKKSNAPKSNGSRSAEREPLLPNPSLNLAAKTGRPTEDSLLFA
                     WDQELRERAAAFTHSDTWRVLRIMGEFVSGFDALAEIGPAVTIFGSARVGPSHPMYKA
                     AREVGKLLAKAGLATITGGGPGIMEAANRGAAEAGGTSVGANIELPMEQGVNRWVNVP
                     LNFRYFFVRKTMFVKYAEGFIIFPGGYGTLDELFESLTLIQTGKLWNFPVVLFGTEFW
                     DGLVDWIEKRLVTDLMIKHEDRNIFRVTDNPQEAVDIMLASFRKHSEQLLLDDLDRMP
                     GDLR"
     gene            3514..>3833
                     /locus_tag="VFI12_06695"
     CDS             3514..>3833
                     /locus_tag="VFI12_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056133.1"
                     /translation="MATVLPPKGPGDTLAIRFISEGDQTAGDIAGQMAEFSRRAEHRL
                     DLAVYDCRLDDATARPIRDALLDRLQSGVKVRLIYDDSKKKPQSHDQFAAIGGDFAEG
                     DTNER"
BASE COUNT          696 a         1280 c         1216 g          641 t
ORIGIN      
        1 gtcctatcca gtggccggca tggtccggga ttggcgaccc gaggggtacg ccgtcgacca
       61 cgcgattgtc ccgcacggca tctcggtcat cctgaatgcg ccggccgtct ttcggttcac
      121 cgcgccggcc gcaccgcacc gccacctgtg cgccgcgcag atgctcggcg ccgacatcac
      181 cgacgcggga ccacaggatg ccggcgaagt gctggccaac cgcttgattg agatcatgcg
      241 catgacgggc atgccgagcg ggttggccga ggtcggttat tccgaagccg atctcgatcg
      301 gttggtcgaa ggcacgctgc cgcagcaccg ggtcacgaag ctctcgccgc gtccggcctc
      361 ggccgacgac ctgcgcggga tctttcacaa cgcgctgcgc tactggtgag ccatgcgtcc
      421 tgtcacagaa gccgaccata gggagcgaat tgagcgggtc gtgacgcagc tccgcgacga
      481 gcccgagcgc acgctgtcac tcaacgagat ggccgatatc gcggcactca gcccgttcca
      541 cgcggtccgg gttttccggt caatcaccgg gtacaccccg gccgccatgc agaccgccgt
      601 ccgggtccag gaagcgaagc gtttgctggt tgccgaagga agctcggtca cggatgcctg
      661 ttacgcagtc ggtttctcga gcctcggcag cttcagccag cgatttgcga cgctaaccgg
      721 ggtcaacccg agcgaatatc gcaccgcggt cgaccgggcc gatgcaatgg ccgacgatct
      781 ggctcgactc gaacgcaggc cgctcctccg catgcacgag cgccataccg tcagcggacg
      841 gatcgtggga gacaccgagc gcgccgcttt ctactttgtc ggcatcttcc cgcccgggcc
      901 gccgcaaggc cggcctttgc gtggcgatgt gctcgacgag ccggggagct tcgtgatccg
      961 acatgtgccg gatggggttt actcgatcta ctgcgccgcc gtcgcccatc cccagcattc
     1021 gatcgactgg gtcatgcccg acacggagtg gcagacgggc ggtgggcaga aggtgcgtat
     1081 gatctgcggg gtgcctgtcc actcgctcga gatcgagatg cgccaccgcc gcccggtcgt
     1141 tgtgccgctg atgacgaccg tgatggcgat cccgaacctg gcggacgatg cgcgcctcaa
     1201 tcacaccctg ctggcatagc aggacgatcc tcaggcagtc atccggaccc ggtcatcggc
     1261 gatcaggccg accggaaccc ggccgccaat cattcgctcg acgattgggc caacgaggtc
     1321 gggttcgcca ctggtatgga tccggagctc cggtatggcg gcaatgccgg ccagcagacc
     1381 ggcccgcatc agcacctcgc gcgctcgtcg ggcaacggcg ttaccgctgt ctacgatccg
     1441 cacggccggc ccggccagct catggatcac ggggcggaca aacgggtaat gcgtgcagcc
     1501 gagcacgatg acgtcgacac catccgcaac ctgcgactgc acccggtccg ccaatagcgc
     1561 atgcaccgcc ggcccatcga cgtcgcctgc ctcgatacga tcggcaagac ccggcacgcc
     1621 gatcggccgg atatcgaatc cggcgccgta ccgcccgatc agcccccgca atctcggact
     1681 tgcggccgtg ctcggcgtgg ccagcacaac gatccggccg gtccgggtca gggcgatggc
     1741 cggcttcacc gccggctcca gcgccacgat cgggaccata aattcggcgc gaaggagctc
     1801 gatggcggcc gcgctggcgg tgttgcaggc tacgatgacc agctttgccc catgcgcgac
     1861 caggtatctc gtcatcgccc gactgcgctc ctgaatctcg tcatgcgagc gctggccata
     1921 ggggcaaaag cggctgtcgg ccgcgtagag aatcgactcg cgcggcagct cgatccggag
     1981 ctgttcgacg acgctcaggc cgcccaatcc ggaatcgaag acgccgatcg gcgcggacgg
     2041 gtgtacgttc atgtacccat cctagcatcg tgcttgccga aaaggaaacc gggaacggag
     2101 ttagatcagg gtgaccgcgt agtgccgggt catgtaccgg tccagttgct caaccgtgtc
     2161 gatcagggca ctctctccgc gggccagggc acgactcaac agcacgccga tcgcttcccg
     2221 ctgggactgg taggccgccc gccgaacctc ggtgtagatg catccgttgg gatcgaacgc
     2281 cagctcagca accagccact gggcctcttc gatatcgagc aggaggagtt ggttgccgtt
     2341 ctgccaggca aacggctgag gcgccggccg gagaagatgc agtgcgcggt ccagcgcgac
     2401 accgccatcg ttacccgcgg atggcgaaat ggggtgctct cgagatggtc gcgcaaacgc
     2461 cgcgtgatcc atggcaatgc cctgcataca gagacattct cggggcagaa cgtcgactgc
     2521 ggtaaccgac ttcgttcggt tcgtgtccga caggcaaagc gtatcactcc accgtgtctc
     2581 gacacaacag tctgggcaac tggccggagt acagttcctc ctatagtcct gcctgttccg
     2641 gcaattctgt gagggagtgg ctgcgtctcc atgccgaaga aatcaaacgc tccgaagagc
     2701 aacggatccc gatccgctga acgcgagccc ctgttaccga acccgagcct caacctggcc
     2761 gcgaaaaccg gtcgaccgac cgaagacagc ttgctcttcg catgggatca ggagttgcgg
     2821 gagcgcgccg ccgcctttac ccatagcgac acctggcgtg tgctccggat catgggcgag
     2881 ttcgtcagcg ggttcgatgc cctcgcggag attggtccgg cggtcacaat ctttggctcg
     2941 gcccgcgtcg gaccgagtca cccgatgtac aaggcggcgc gagaggtcgg caagttgctc
     3001 gccaaggccg gcctggccac gatcacgggc ggcgggcccg gcatcatgga agcggccaac
     3061 cgcggcgcgg ccgaggccgg cggcacctcg gtcggcgcga acatcgaact gccgatggag
     3121 cagggcgtca accgctgggt caatgttccg ctcaacttcc gctatttctt cgtgcgcaag
     3181 acgatgttcg tcaagtatgc cgagggcttc atcatcttcc ccggcgggta cggcacgctg
     3241 gacgagctct tcgagtcgct gacgttgatc cagaccggca agctctggaa cttcccggtc
     3301 gtcctcttcg gcacggagtt ctgggacggg ctcgtggact ggatcgagaa gcggctggtc
     3361 accgacctga tgatcaagca cgaagatcgc aacattttcc gggttaccga caatccccag
     3421 gaggccgtcg acatcatgct cgcctcgttc cgcaagcact cggagcagct cctgctcgac
     3481 gacctcgacc ggatgccggg ggacctgcgc tagatggcga ccgtcctccc acccaaaggc
     3541 ccgggtgaca cgctcgccat ccgcttcatc tccgagggcg accagactgc cggtgacatt
     3601 gccggacaga tggccgagtt cagtcgccgt gccgaacacc ggctcgatct cgcggtctac
     3661 gactgccggc tcgacgatgc gaccgccaga cccatccgcg acgcgctcct ggaccgcctg
     3721 caaagcgggg tcaaggtccg gctcatctac gacgacagca agaagaagcc gcaatctcac
     3781 gatcagttcg ccgcgatcgg cggcgatttc gcggagggcg acacgaacga acg
//
LOCUS       DASSGX010000375         3203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_720558, whole genome shotgun sequence.
ACCESSION   DASSGX010000375 DASSGX010000000
VERSION     DASSGX010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720558"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1829)
                     /locus_tag="VFI12_06700"
     CDS             complement(<1..1829)
                     /locus_tag="VFI12_06700"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HET7056134.1"
                     /translation="MRVPGHIARTRRGTLQRSRSSQARWLNAVTAMLLLAGLLANFVT
                     IAPLPVAAQQKDNTPPPYPTPQSVVAVGSFQTAVGCGQDFDKDCDLTQLQANSDGTWT
                     GTFQIPAGSYTYRIVTRSDIDRSFGKDGDPNGDDISLDVPDGALGVFFSFNEWTGEIY
                     AVPVTNQIELSTDLGQFPMQPNKDGGAFAVIDAQPNSSFGAHVILDGNPVGDVVTVDT
                     GDSGRVVVTMDQNGSLKSDSVEQSTLTVTKTDADGNPLPGACFSVYDGKDVAGQGCDS
                     SDGEDGSTRIEFPVGAGNGLELRETRTPDGQERADSQDIDLNPGDNQVTVTAGGGGGQ
                     ITPPEEETATAVEEETPTEEVTPELGTITLYTVDENGNPVPGVCYTVSSYSDVQCDED
                     GDGDMGLTDVPAGDYTAQQASVPDGYQLDNQEQTITVSGGEEATGTFTSPTTQAQTFT
                     VTVTITDQDGNPVPGACFTVTDANANQGSEQCDDDQNGDVQFDLANGDYTLSMTTTPD
                     GYETAEDQSFTVSDQDVDLQVSVNAVAPSETATTGGEGNGAINVSVTDQDGFAIIGAC
                     VTIQGPTSGEVCDNGDGDANGEDGLIRIENLPDGDYTVTVSQLP"
     gene            complement(2086..3144)
                     /locus_tag="VFI12_06705"
     CDS             complement(2086..3144)
                     /locus_tag="VFI12_06705"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET7056135.1"
                     /translation="MRLALETGPELWRTPDGVAKSVELAQLAEQLGFDSIWASEDPDG
                     WDAFSALTVLARATERLHLGTGVTNPYYRHPNLISASVATLDRASDGRAFLGLGRGEP
                     DWYRTAFGMELGSPLEHVRETVDLLRQWWGPDQTANSDGEITVRSWRREFSPLGNPPI
                     YLAATGRKMQALAGRIADGLRLNALGSLPFLRRSVETFLDSARSVNHDPADLRIFANP
                     GLTVTATDAEIEPALERKKTTVALIHALPGMEQQLEGLEAEFDLEAILARVRRHMRTE
                     EILARGGSFADLRREGDLDAARKAIPLELVDRVAVVGPPERVLPRLTEYAELGITDIF
                     ATPRQLQDDQLRIAIRSL"
BASE COUNT          501 a         1030 c         1094 g          578 t
ORIGIN      
        1 ggaagctggc tcacggtcac ggtgtaatca ccgtccggca ggttctcgat ccggatcagt
       61 ccgtcctcgc cgttagcgtc gccgtcgccg ttgtcgcaga cctcacccga cgtcgggccc
      121 tggatcgtga cgcaggcgcc gatgatcgcg aaaccgtcct ggtcggtcac ggagacgttg
      181 atcgcgccat tgccttcgcc accggtcgtc gccgtctcag acggggcaac cgcgttcacg
      241 gagacctgca gatcgacatc ctggtcggaa acggtgaagg actggtcctc agccgtctcg
      301 tagccatccg gtgtcgtcgt catcgagagc gtgtagtcgc cgttggcgag gtcgaactgg
      361 acatcgccgt tctggtcgtc gtcgcactgc tcgctgccct gattagcgtt ggcgtcagtg
      421 acggtgaagc aggcaccggg gaccggatta ccgtcctggt ccgtgatcgt taccgtgacg
      481 gtgaaggtct gggcctgggt cgtcgggctg gtgaaggtgc ctgtcgcttc ctcaccgccg
      541 ctgaccgtaa tcgtctgttc ctggttgtcg agctggtagc catccggcac cgaggcctgc
      601 tgggcggtgt agtcgccggc cgggacatcg gtcagcccca tgtcgccgtc gccatcctcg
      661 tcgcactgga cgtcgctgta actgctcacg gtgtagcaga caccggggac cgggttgccg
      721 ttctcgtcga cggtgtagag ggtgatggtg ccgagctcgg gcgtgacctc ctcagtcggg
      781 gtctcctctt cgacagccgt ggccgtctct tcctcagggg gggtgatctg gcctccgccg
      841 ccaccggccg taaccgtcac ctggttgtcg ccggggttga ggtcgatgtc ttgcgagtcg
      901 gcccgctcct ggccgtcggg cgtgcgcgtc tctcgcagct cgaggccgtt gccggcgcca
      961 accgggaact cgattcgggt cgagccgtcc tcgccatcgc tggaatcgca gccttggccg
     1021 gccacgtcct taccgtcgta gacggagaag caggcgcccg gcagcgggtt gccatcggcg
     1081 tcagtcttgg tcaccgtcaa ggtggactgt tcgaccgaat ccgacttcag gctgccgttc
     1141 tggtccatcg tgaccacgac gcggccggaa tcgccggtat cgaccgtgac gacatcgccg
     1201 accggattgc catcgaggat tacgtgcgcc ccgaacgagc tgttcggttg cgcgtcgatc
     1261 acggcgaagg cgccgccgtc cttattgggt tgcatcggaa actggccgag atcggtcgag
     1321 agctcgatct ggttcgtgac cgggacagcg tagatctcgc cggtccactc attgaacgag
     1381 aagaagacgc cgagcgcgcc atccggcacg tcgagcgaga tgtcgtcacc gtttgggtcg
     1441 ccgtccttgc cgaaggagcg gtcgatgtcc gaccgggtca cgatgcggta ggtgtaggag
     1501 ccggccggga tctggaacgt gccggtccag gtgccatcgc tgttcgcctg gagctgggtg
     1561 agatcgcagt ccttgtcgaa atcctggccg cacccaaccg ccgtctggaa cgatccaacc
     1621 gcgaccacgc tctgcggggt cgggtacggt ggcggcgtgt tgtccttttg ctgcgcggca
     1681 accggcagcg gcgctattgt cacgaagttg gccagcagcc cggccagcag gagcatggcc
     1741 gtcacggcat tcagccagcg agcctgggag gaacgcgacc gctgcaacgt tccgcggcgg
     1801 gtgcgagcaa tgtgaccagg aacgcgcatc gaatcgacct ccccagatct ggacggcttc
     1861 tctgccgaat ccttcaagag cgtgtctcgt tcccgatccc ccgggacggt gcaacgaccg
     1921 tgtggtacgg atggggagat cggggctccc gaaggcgacc gtacgtcgat ggctcatcat
     1981 acccgacgtt ccagcgaccg ctttccccgt ccctaccaag gacgggcagg ttgcatctcc
     2041 cataacgcga gggctcgcac aggggtttcg gaaaggagac ggggattaca gcgagcggat
     2101 cgcgatgcgc aattgatcgt cctgaagctg acgcggagta gcgaagatgt cggtaattcc
     2161 gagctcggcg tactcggtca accgaggcag cacccgctcc ggcggaccaa ccacggccac
     2221 gcgatcgacc aactcgagcg ggatcgcctt tcgggcggcg tcaaggtcgc cctcccgccg
     2281 caaatcggcg aagctgccgc cccgggcgag gatctcctcc gtccgcatat gcctccgcac
     2341 tcgcgccagg atcgcctcga ggtcgaactc ggcctccagc ccctcgagct gctgctccat
     2401 cccgggcagc gcgtggatca aggcgacggt cgtcttcttg cgctccaggg ccggctcaat
     2461 ctcggcgtcg gtcgcggtga ccgtgagtcc ggggttcgcg aagatgcgaa gatcggccgg
     2521 gtcgtggttc acggaccggg cggaatcgag gaaggtctcg acgctccgcc tgaggaaggg
     2581 cagcgagccg agtgcgttga gccgcagccc gtccgcgatc cggccggcca acgcctgcat
     2641 cttgcggccg gtcgcggcga ggtagatcgg cgggttgccg agcggcgaga actcgcgccg
     2701 ccagctccgc acggtgatct cgccgtcgct gttggcagtc tggtccggtc cccaccactg
     2761 ccgcagtagg tcgacggtct cgcggacatg ttcgaggggc gagccgagct ccatcccgaa
     2821 cgcggtgcga taccaatcgg gctcgccgcg gccgaggccg aggaaggcgc ggccatccga
     2881 ggcgcggtcg agcgtcgcca ccgaggccga gatcaggttc gggtggcgat aataaggatt
     2941 ggtgacgccg gtgccgagat ggaggcgctc agtcgcgcgg gcgagaaccg tcagggcgct
     3001 gaaggcgtcc cagccgtcgg gatcctcact cgcccagatc gagtcgaagc caagttgttc
     3061 ggcaagttgg gcgagctcga ccgatttcgc gacgccgtcc ggcgtgcgcc agagctccgg
     3121 tccggtttcg agcgcgagcc gcatcggtta tcgctgcggg cggtagtaga agacgatccc
     3181 gcgcggccgc tcgtcaccgg tcc
//
LOCUS       DASSGX010000376         2857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_829764, whole genome shotgun sequence.
ACCESSION   DASSGX010000376 DASSGX010000000
VERSION     DASSGX010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829764"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2832)
                     /gene="rpoB"
                     /locus_tag="VFI12_06710"
     CDS             complement(<1..2832)
                     /gene="rpoB"
                     /locus_tag="VFI12_06710"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000428 - DNA-directed RNA polymerase
                     complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET7056136.1"
                     /translation="MAEVYCRERDMTYSARLRISARLVINETGEVKETGPDGIFIGDF
                     PMMTSDGTFIINGAERVVVSQLVRSPGVYFDRNTDPTTGKLVSTAKLIPNRGAWLEFE
                     TSNRDVISVKVDRKRKMPVTILLRAIGLETDEALRAAFADVDTNEDRKYIQTTMEKET
                     TKSRDEALIELYRRLRPGDPPTKDNASTMLENLFFNDRRYDLARVGRHKLNQRLHGEK
                     QGTDDQISTRILTVEDLVEIIREMIRINNGQGQGDDIDHLGNRRIRAVGELIQMHVRT
                     GFQRLERGIRERMTIQDPETVTPQSLISTTRPVMAAVREFFGGSQLSQFMDQTNPLAE
                     LTHKRRLSALGPGGLSRDRAGFDVRDVHSSHYGRICPIETPEGPNIGLIGSLATYGKI
                     NQFGFIETPYRRVVSSITLDDPVIDPVGQFLRDDAVNPENGKVVLASGSELTAANVES
                     LKKAGVKLVRIQAVASNLIDYLSADKETNVSIAQASTPLEVNGRIIPDSVTVRVGGSG
                     HSFPQIPAEQVAYMDVSPKQVVSVATALIPFLEHDDANRALMGANMQRQAVPLVRPRS
                     PIVGTGVEHQAAKDSGQVLVSRHNGTVKHVNSREVVIVEDDGAEHIYQLRKFVRSNQD
                     TCINQRPSVSKGDRVTAGQIVADSSSTENGELALGQNVLVAFMPWEGGNFEDAILISE
                     RLVRDDVFTSIHIEKYETEARDTKLGPEEITRDIPNVGEESLANLDENGIIRIGAEVR
                     PNDILVGKVTPRGETELSAEERLLRAIFGEKAREVKDTSLRVPHGVHGKIIDVKVFRR
                     DESPDHELPAGVNEMVRVSIAQKRKISEGDKMAGRHGNKGVISRILPIEDMPYLPDGQ
                     SVDIILNPIGVPSRMNLGQVLETHLGWAAERLGYKVATPVFDGAKETEIRKALVDAGL
                     PEDGKVDLYDGRTGEKFDRPVTVGMIYMLK"
BASE COUNT          471 a          940 c          877 g          569 t
ORIGIN      
        1 cttgagcatg tagatcatgc caaccgtaac cgggcgatcg aacttctcac cggtgcggcc
       61 gtcatagaga tcgaccttgc catcctctgg cagacccgca tcgaccaggg ccttgcgaat
      121 ctcggtctcc ttcgcgccgt cgaagaccgg cgtcgcgacc ttgtacccga gccgctccgc
      181 cgcccagccg aggtgtgtct ccagcacctg accaaggttc atacgtgacg gcacaccgat
      241 cgggttgagg atgatgtcga ccgattgccc atcgggcagg tacggcatgt cctcgattgg
      301 caggatccgg ctgatcacgc ccttgttgcc gtgccggcca gccatcttgt cgccttccga
      361 gatcttgcgc ttctgcgcga tgctgacgcg caccatctcg ttgacgcctg ccgggagctc
      421 gtgatccggg ctctcgtcgc gcctgaagac cttgacgtcg atgatcttgc cgtggacacc
      481 gtgcggcacg cgcaacgagg tgtccttcac ctcgcgcgcc ttctcaccga agatcgcgcg
      541 gagcaggcgc tcctcggcgc tcagctcggt ctcgccacgc ggcgtgactt tgccaaccag
      601 gatgtcgttc ggtcgaactt cggccccgat tctgatgatg ccgttctcgt cgaggttcgc
      661 caggctttcc tcgccgacgt tcggaatatc ccgagtgatc tcttccggac cgagcttggt
      721 atcccgcgcc tctgtctcgt acttctcgat gtggatcgag gtaaagacgt cgtcgcggac
      781 gagtcgctcg ctgatcagga tcgcgtcctc gaagttcccg ccttcccacg gcatgaacgc
      841 caccagcaca ttctggccaa gggcaagctc gccgttctcg gtcgacgacg aatcggcgac
      901 gatctggccc gcggtcaccc ggtcgccctt gctcacgctc ggacgctggt tgatgcaggt
      961 gtcctgattg gaccgcacga acttccgaag ctgatagatg tgctcagccc catcgtcctc
     1021 aacgatcacg acctcgcgcg aattgacgtg cttgaccgtg ccgttgtgac gggagacaag
     1081 cacctgcccc gagtccttcg cggcttgatg ctcgacgccg gtgccgacga tcggcgaccg
     1141 cggacgaacc agcggaaccg cctggcgctg catgttcgcg cccatcaagg cgcggttcgc
     1201 atcgtcatgc tccaggaacg ggatcaaggc cgtcgccacc gaaacaacct gcttcggcga
     1261 gacgtccatg tacgcaacct gctcggccgg gatctgcggg aagctgtgac cacttccacc
     1321 gacgcgaaca gtgacactgt ccggaatgat ccggccattc acctcgagcg gcgtcgacgc
     1381 ctgcgcgatc gacacgttgg tctccttgtc cgcggacagg tagtcgatca gattgctggc
     1441 gactgcctgg atgcgaacca gcttgacgcc ggccttcttg agcgactcga cattcgccgc
     1501 ggtcaactct gagccggacg caagcacgac cttcccgttc tccgggttga ccgcatcgtc
     1561 gcgcaggaac tgcccgaccg gatcgataac cgggtcgtcc agggtgatcg acgaaaccac
     1621 ccggcgatac ggcgtctcga tgaagccgaa ctggttgatc ttgccgtagg tcgcgagcga
     1681 gccgatcaga ccgatgttcg ggccctccgg cgtctcgatc gggcagatcc ggccgtagtg
     1741 cgacgagtga acgtcgcgga cgtcgaagcc tgcgcggtcc cggctgagac cgcccggacc
     1801 gagggccgaa agccggcgct tgtgcgtcaa ctcggccagc gggttcgtct gatccatgaa
     1861 ctgcgagagc tgcgaccctc cgaagaactc gcgcacggcg gccataaccg gccgggtcgt
     1921 gctgatcaag ctctgcggcg tgaccgtctc ggggtcctgg atcgtcatgc gctcccggat
     1981 gccgcgctcg aggcgctgga agccggtgcg gacatgcatc tggatcaact cgccgaccgc
     2041 gcggatgcgg cgattgccga gatggtcgat gtcgtcgccc tggccctgac cattgttgat
     2101 ccggatcatt tcgcggatga tctcgaccag gtcctcgacc gtcaggatgc gggtcgagat
     2161 ctgatcgtcg gttccctgct tctctccgtg caggcgctgg ttgagcttgt gccggccgac
     2221 tcgcgccagg tcatagcggc ggtcgttgaa gaacagattc tccagcatcg tggaggcgtt
     2281 gtccttcgtc ggcggatcgc ctggacgcag ccgacgatag agctcgatca aggcctcatc
     2341 gcggctcttg gtcgtctcct tctccatcgt cgtctggatg tacttgcgat cctcgttggt
     2401 atccacgtca gcgaatgccg cgcgcagagc ctcatccgtc tccaggccaa tggcacggag
     2461 caggatcgtg accggcatct tgcgcttgcg atccaccttg actgagatga catcgcggtt
     2521 agacgtctcg aactccagcc aggcgccccg gttcgggatc agcttggccg tggagacgag
     2581 cttgcccgtg gtcggatcgg tgttgcggtc gaagtagacc ccgggcgagc gcaccagttg
     2641 ggagactacc acccgctcgg cgccgttgat gatgaaggtg ccgtcggacg tcatcatcgg
     2701 gaagtcgccg atgaagatgc cgtccggacc ggtctccttc acctcgccgg tctcgttgat
     2761 aaccagcctg gcggagatcc ggagccgggc cgagtaggtc atgtctcgct cgcggcagta
     2821 gacctccgcc actcgcggat cctgctttgc tcgctcc
//
LOCUS       DASSGX010000377         2111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1157152, whole genome shotgun sequence.
ACCESSION   DASSGX010000377 DASSGX010000000
VERSION     DASSGX010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157152"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..468)
                     /locus_tag="VFI12_06715"
     CDS             complement(7..468)
                     /locus_tag="VFI12_06715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HET7056137.1"
                     /translation="MREVLGCFATGVAVVTTLGDRWAPVGVTINSFNSVSLEPPLVLW
                     SLALESPSLGAFRAHRAFAINILSVDQARIGRQFANPAPDKFAGVDWEPGFRGVPLLA
                     DAAARLECRTWRRYEGGDHEIIIGEVIAMAATEKAPLVFHRGRFARVDELR"
     gene            641..1579
                     /locus_tag="VFI12_06720"
     CDS             641..1579
                     /locus_tag="VFI12_06720"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET7056138.1"
                     /translation="MSNLKLQQIRLFVAVYEEGSFTAAARREHATQSGLSMQIKDLET
                     ALGVRLFDRLPTGVVPTVVGRRLYEHAIRILREVGHLTDGIATLSGAITGEVHVGLMP
                     TFTRAILAPALLSFSAAHPHVNVRVTEAYSGVLTQEVAKQALDFAIVPPGHAGRTIRS
                     RRLGTDREYLVTRPDTHRTHLSPIELSRESIPIRLIVPGPSNTRRGRIDAYLETCGVP
                     VAAILEMDAMMGTLDLVARSDWVTILSGVLCAPDRDGRTRKLHPLVNPELNVDYMLIE
                     PSTRSLSPAARLFAEAITVEMLRLIDQATCSGHQHL"
     gene            1674..>2111
                     /locus_tag="VFI12_06725"
     CDS             1674..>2111
                     /locus_tag="VFI12_06725"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET7056139.1"
                     /translation="MEVSFFTMPVHPLDKPIAQTLKEDREAFLLADELGYAEAYCGEH
                     TTDLAENIPSCVAFLASLAYQTKRIRLGTGTVNLPNSHPAAVASQVAMLDHMLEGRLN
                     FGISPGGLMSDAEVFGNLDRDRNAMFVEGINMVLDIWAGEAPYD"
BASE COUNT          370 a          723 c          647 g          371 t
ORIGIN      
        1 cgcgtctcaa cgaagctcat ccactcgggc gaagcgaccc cgatgaaaga cgagcggagc
       61 cttctccgtt gccgccatcg caatcacctc gccgatgatg atctcatggt ccccgccttc
      121 gtagcggcgc cacgtcctgc attccagtcg cgcggcggcg tcggcaagca gcggcacgcc
      181 gcgaaatccg ggctcccagt cgacgccggc gaacttgtcc ggcgcagggt ttgcgaactg
      241 ccggccgatc ctggcctggt ccacggagag gatgttgatc gcgaatgccc ggtgagcgcg
      301 gaacgcgccg aggctgggtg actccagcgc taggctccac agaacgagcg gcggctccag
      361 ggagacggag ttgaaggagt tgatcgtcac gcctaccggc gcccaacggt cgcccagggt
      421 cgtcaccacg gcgacgccgg tcgcgaagca gccgaggacc tcgcgcaact cgcgagtccc
      481 gatcgagggc gaggtctcgt cggctccgca caggtcttcg gcagaggcta tccggtcggc
      541 attcgcgcag gtcattgcgc gaacataaga tcgatgctgt atcagctcaa attctattgc
      601 ctaatgctgg ctatcagatc gactgattcc gactggtgcg atgtccaatt tgaagctcca
      661 gcagatccgg ctgttcgtgg ccgtctacga ggagggctcg ttcaccgcag ccgcccggcg
      721 cgagcacgcg acgcaatccg ggctgtccat gcagatcaag gatctggaga cggcgcttgg
      781 agtgcggctg ttcgaccgac tgccgacggg cgtggtgccg accgtcgtcg gccggcgcct
      841 gtacgagcac gcgattcgca ttctgcgcga ggttgggcat ctcaccgacg gcatcgccac
      901 tctgtcgggc gcgatcaccg gcgaggtcca cgtcggtctg atgccgacct ttacgcgtgc
      961 cattctggcg ccggcgctcc tgtccttctc cgccgcgcac ccgcacgtca acgtccgcgt
     1021 gaccgaggcg tacagcggcg tgctcacaca ggaggtcgcg aagcaggcgc tggacttcgc
     1081 aatcgtgccg cccggtcatg cgggcaggac catccgctcg cgccgcctcg gcaccgaccg
     1141 ggagtatctg gtcacgcgcc cggacaccca ccgcacgcac ctgtcgccga tcgagctgtc
     1201 acgagagtcg attccgatac gactgatcgt gcctgggcct tccaacaccc gccgcggccg
     1261 gatcgatgcc tatctcgaga cctgcggcgt gcccgtcgcc gcgattttgg agatggacgc
     1321 aatgatgggc acgctcgacc ttgtcgcccg ttccgactgg gtcacgatcc tgtcgggcgt
     1381 gctgtgcgct cccgaccgcg acggcaggac ccgtaagctg caccccttgg tcaaccccga
     1441 gctgaacgtc gactacatgc tcatcgagcc aagcacccga agcctgtcgc cggccgcgcg
     1501 cctcttcgcc gaagcgatca ccgtcgagat gctgcgactg atcgatcagg ccacctgctc
     1561 tgggcatcag catttgtgaa cgcgcagata atgaaagatc attacttctg ctggacgcta
     1621 caccttatcg tctcgtctcc ttctcattcc accgagccgt gcggagccga tacatggaag
     1681 tgtccttctt caccatgccc gtccacccgc tcgacaagcc tattgcccag accctcaagg
     1741 aggatcgcga ggcattcctg ctggctgacg agctgggcta tgccgaggcc tactgcggag
     1801 agcacaccac ggaccttgcc gagaacatcc cgtcctgcgt cgccttcctc gccagcctcg
     1861 cctatcagac gaagcgcatc cggctcggca ccggcacggt gaacctgccc aacagccatc
     1921 ccgcggcggt ggcgagccag gtggccatgc tcgaccacat gctcgaaggg cggctcaatt
     1981 tcggcatcag cccgggcggg ctgatgtccg atgccgaggt gttcggcaac ctggatcgcg
     2041 accgcaacgc catgttcgtc gaagggatca acatggtcct cgacatctgg gcgggcgagg
     2101 caccttacga c
//
LOCUS       DASSGX010000378         2029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_940888, whole genome shotgun sequence.
ACCESSION   DASSGX010000378 DASSGX010000000
VERSION     DASSGX010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940888"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..1194
                     /locus_tag="VFI12_06730"
     CDS             169..1194
                     /locus_tag="VFI12_06730"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7056140.1"
                     /translation="MSRFAVGIGSDFYDAEGIPKLPALRTAFLDLPEDVDIVTVQCSG
                     GAYQCDVIHDVDALLMSGGACDASSLLNNDRLAVVCRWGVGYDTVNLKDCAEAGIVVA
                     NAPEGVRRSMAQTAMAFVLALAHRLFDQDRAMRSGGCWPIKHQYIGTGLVGKTLGVVG
                     LGNIGREILRVASVFDCEVIGYDPYADLSAGPVTRVELDELMRRSDFIILQCALTPET
                     RGMINRERIAMMKPTAYLVNTSRGPVVEEPALIEALQEKKIAGAALDVFMREPLEEDS
                     PLLKLDNVILTPHSAGWTDDFARRTAESISQTIRSIMNGELPANAVNKRDLEESGVKP
                     RYLRYRQ"
     gene            1191..1964
                     /locus_tag="VFI12_06735"
     CDS             1191..1964
                     /locus_tag="VFI12_06735"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HET7056141.1"
                     /translation="MSPSELKQALADGQVVAGTMVFEFNTPGIGLIAAEAGAEFVLYD
                     MEHTGWSIESIRAQMAWSRSAPITRLVRVPATQYDYISRALDVGAQGVMAPMVESAEQ
                     ATAIATASSYPPEGRRGAAFGIAHDGYAPGAVLDKMRAANAERVMIAQVETITGVKNA
                     REIAAVPGIDILWVGQFDLTVSLEIPGQFDHPDFVAAVKAVADAARNAGKACGAMALS
                     VDDAKTWVSRGYNMIAYSGDLWIYQLALTGSLRALRDAL"
BASE COUNT          369 a          614 c          701 g          345 t
ORIGIN      
        1 cgacaagcat cgccggaagt atggacgcga gtttgagcaa acgagaaacg ggcacgtcca
       61 atcctggcga tcgtggggcc ggtgtccgcc gagtggcggt cggatagtag gtgtccgttc
      121 gggccagcgt caatgcggcc ggcccgaccg cgaggcaggg gagcgttaat gagcaggttc
      181 gccgtcggta tcgggtccga tttctacgac gcggaaggca tccccaagct gccggcgctg
      241 cggaccgcct tcctcgatct gcccgaggac gtcgatatcg tgacggtcca atgctctggt
      301 ggtgcgtacc agtgcgacgt gattcacgat gtggacgcgt tgctgatgtc cggcggggct
      361 tgcgacgcct cgagtctgct caacaacgac cggctggccg tcgtttgccg ctggggcgtc
      421 gggtacgaca ccgtcaacct caaggattgc gccgaagctg gcatcgtcgt cgcaaatgcg
      481 cccgagggag tgcgacggtc gatggcccag accgcaatgg ccttcgtgct cgcactggcg
      541 catcgcctct tcgatcagga ccgggccatg cgaagcgggg gctgctggcc gatcaagcat
      601 cagtacatcg gcaccggcct ggtgggcaag acgctcggcg tcgtcggcct cggcaatatc
      661 ggccgggaga tcctgcgggt cgcgtcggtc ttcgactgcg aggtgattgg ctacgacccc
      721 tatgccgacc tcagcgccgg tccggtcacc cgggttgagc tggacgagtt gatgcgcagg
      781 tccgacttca tcatcctgca atgcgccctg acgcccgaga ctcgcggcat gatcaatcgc
      841 gagcgaatcg caatgatgaa gccaaccgcc tacctcgtga acacgtcgcg cgggccggtc
      901 gtcgaggagc cggcactgat cgaggcgctt caggagaaga agatcgcggg cgcggcgctc
      961 gacgtcttca tgcgggagcc gctcgaggaa gacagtccgc tcctcaagct cgacaacgtc
     1021 atcctgacgc cgcactcggc cggctggacc gatgatttcg cgcgtcggac agccgagagc
     1081 atctcgcaga cgatccggtc gatcatgaac ggcgagctgc cggcgaacgc ggtcaacaag
     1141 cgcgatctcg aagaatcggg cgtcaagccg cgctatttgc gatatcgcca atgagcccgt
     1201 cggagctgaa gcaggcgctg gccgacggac aggtcgtcgc cggcacgatg gtcttcgagt
     1261 tcaatacgcc ggggattggc ctgattgccg cggaagcagg cgccgagttc gtcctctacg
     1321 acatggagca taccggctgg agcatcgagt cgatccgggc acagatggcc tggtcgcggt
     1381 cggcgccgat cacccggctc gtgcgcgtgc cggccacgca gtacgactac atcagccggg
     1441 cgctcgatgt cggtgcgcag ggcgtaatgg cgccgatggt cgagtcggcg gagcaggcga
     1501 cggcgattgc gactgcgtct agctacccgc cagaaggtcg ccgcggcgcc gccttcggga
     1561 tcgctcacga tggatacgcg cccggcgcgg tcctcgacaa gatgcgcgcc gccaacgcgg
     1621 agcgggtcat gatcgcgcaa gtcgagacaa tcaccggcgt gaagaacgcg cgcgagatcg
     1681 cggcggtacc cggaatcgac atattgtggg tcgggcagtt tgacctgacg gtttcgctcg
     1741 agatcccggg gcaattcgac catccggatt tcgttgcggc ggtcaaagct gtagccgatg
     1801 cggccaggaa cgcagggaaa gcgtgcggcg cgatggcact gtcggtcgac gacgcgaaga
     1861 cctgggtatc gcgtgggtac aacatgatcg cgtactcggg cgacctctgg atttaccagc
     1921 tggcgctgac ggggtcactg agggcgctca gggatgcatt gtgacgggat tcccacgcgg
     1981 tgggcgatag tggactcgaa ccactgacct ctacgatgtc aacgtagcg
//
LOCUS       DASSGX010000379         3677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_175539, whole genome shotgun sequence.
ACCESSION   DASSGX010000379 DASSGX010000000
VERSION     DASSGX010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175539"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VFI12_06740"
     CDS             complement(<1..465)
                     /locus_tag="VFI12_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056142.1"
                     /translation="MRIESFTATPDLIGRRVRLSWTFTPDFGETIADVPRVTVRRKRR
                     DFAYPPPAPGDPYLVYDSATFPPAPIPGSLLVRDLPDRNMIDGPQRIREQTTTVAAID
                     AGITHEVLRRTLRVVFGPDRTPLREEVELIDIGGIGGSLDPGDNYFYEIDSPI"
     gene            complement(462..2066)
                     /locus_tag="VFI12_06745"
     CDS             complement(462..2066)
                     /locus_tag="VFI12_06745"
                     /inference="COORDINATES: protein motif:HMM:NF016736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HET7056143.1"
                     /translation="MLSPRPAIDYTDKDFRSLRDAMLRLAQDRLPEWTDRSPADLGML
                     FVDLFAYMGDITLYYQDRIASELFPSTAVERRSIVDLLRLIGYDLSASSPARADLLLT
                     FTAPVGPQTVTVPNGARFSARPANASPVEFVYLGPDLAITLSSDQVRPALDAAGNAVV
                     LYEGLPVEQSVEAGPLIIGSSTGEPNQVFALPGAGVVRESVLVEVDEGSGWVVWDRSD
                     SLLFDIAPDGRAILSHPDARHYQLIVDAADVTNVLFAADRQPPIGTNNIRASFRRTQG
                     AAGNVAAGTINTLVTPVPALVAVINPGDAAGGSDAERSDHAIRYAPLAYRSNNRAVTI
                     SDYVALAQSTGAIAKVRAQSQAWNQIDLYVAPAGPVCTPVPEALRNWLLGYMEDKRMA
                     GTFVRVLDAHCVPIDISIELIIEERFAADSVISRVYDAVAQLLAFDRVDFAEKLFQSD
                     VYAAAEGVAGVIAVTVKRFRRRDQPATDLEAELARYNLPSLAELPDFLRNAISSEIAP
                     EGRIEAGEFEIPSIGDLSITTRPSTS"
     gene            complement(2080..2445)
                     /locus_tag="VFI12_06750"
     CDS             complement(2080..2445)
                     /locus_tag="VFI12_06750"
                     /inference="COORDINATES: protein motif:HMM:NF016829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HET7056144.1"
                     /translation="MEDASGPAFPFRIDSRTGGIAWETGANKIRDDLRILIGTRLGER
                     PMLREFGTRVHSLVHENNDDVTADLIRRQAHEAVVQWERRVVVTRASVQQREAELRLV
                     LNYMHSDRPIAGEMIVPIA"
     gene            complement(2459..2677)
                     /locus_tag="VFI12_06755"
     CDS             complement(2459..2677)
                     /locus_tag="VFI12_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056145.1"
                     /translation="MPLNVDDLKNALRQVFADGKSAPDSDTVANDLAQAIHDYIVTAS
                     VNGVVVNVVDGGGHPLGTGTQTTAVAVG"
     gene            complement(3048..>3677)
                     /locus_tag="VFI12_06760"
     CDS             complement(3048..>3677)
                     /locus_tag="VFI12_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056146.1"
                     /translation="VPPVDARVWVEFEEGERTSAIWSGAWYPQGKAPVQAQVSPPESR
                     VIQTPAGHTIQIVDKAGEEQILIQHAKDAFISIDSKGSILISNPNGSHIHLDADGKQA
                     TFVEQHGNYLVMNDKGTAIVNPDGTTVNIAGDTVHLKAGSVVVEAKSVALGMGASDPT
                     IMGNAFDTYWKALLLHTHPTAMGPSGPSIELQPLQLIPGVHLTSSVAVK"
BASE COUNT          620 a         1081 c         1313 g          663 t
ORIGIN      
        1 gatcgggctg tcgatctcgt agaagtagtt gtcgccgggg tccagcgacc cgccgatgcc
       61 gccgatgtcg atcagctcga cctcctcgcg tagaggcgtg cggtcgggcc cgaacactac
      121 gcgcagggta cgccgcagga cttcgtgggt tattccggcg tcgatcgcgg ccacggtggt
      181 cgtctgttcg cgaatccgtt gcggtccatc gatcatgttg cggtcgggca gatcgcggac
      241 gagcagcgat ccagggattg gcgccggcgg gaaggtggca ctgtcgtaga ccaggtaggg
      301 atcgcccggc gccggcggtg ggtaggcgaa gtcgcgacgc ttgcggcgca ccgtgacgcg
      361 tggcacgtca gcgatcgtct cgccgaaatc gggcgtaaac gtccagctga gcctgacgcg
      421 ccgcccgatg aggtccggag tcgccgtgaa gctctcgatc ctcatgacgt agacggcctt
      481 gtcgtgatcg agaggtcacc gatggaaggg atctcgaact cgccggcttc gatacgtcct
      541 tcgggtgcga tctcgctgga aatggcgttg cgcaggaagt ctggcagctc ggccagggaa
      601 ggcaggttgt agcgtgcgag ctccgcctcc aggtccgtgg cgggctgatc gcggcggcgg
      661 aatcgcttca cggtgacggc gattacgccc gcaaccccct cggccgctgc atacacgtcg
      721 ctctggaaca gcttctccgc gaagtcgacg cgatcgaagg cgaggagctg cgcgaccgcg
      781 tcgtagacac ggctaatgac ggagtcggcg gcgaagcgct cctcgatgat cagctctatg
      841 gagatatcga tcggtacgca gtgcgcatcc aggacgcgca cgaaggttcc ggccatgcgc
      901 ttgtcttcca tgtagccaag gagccagttg cgcagcgcct cgggcacggg cgtacacacg
      961 gggccggcgg gcgccacgta taaatcgatc tggttccatg cctggctctg tgccctgact
     1021 ttagcgatgg cgccggtgct ctgagccagg gcgacgtagt cgctgatcgt tacggcgcgg
     1081 ttgttggagc gataggcgag cggcgcgtag cgaatcgcgt ggtccgagcg ctcggcgtcg
     1141 ctgccgcctg cggcatcgcc ggggttgatc accgctacca gcgcaggcac cggcgtaacc
     1201 agcgtgttta tcgtgccggc tgcgacgttg ccggcggcgc cctgggtgcg gcggaagctg
     1261 gcgcgaatgt tgttggtacc gattggcggt tggcggtcgg ccgcgaatag cacgttggtg
     1321 acgtcggccg cgtccacgat aagctggtag tgacgcgcgt ccggatgcga gagaatcgcg
     1381 cgtccgtcgg gtgcgatgtc gaacagcagg ctatcgctgc ggtcccagac gacccaaccc
     1441 gagccttcgt ccacctcgac gaggaccgac tcgcgcacga ctccagcgcc aggcagcgcg
     1501 aacacctggt tcggttctcc cgtcgaggag ccgatgatca gcggtcccgc ttccaccgat
     1561 tgctcaaccg gcagtccttc gtacaggacg acggcgttac ccgcggcatc gagcgccggt
     1621 cgtacctggt cggacgagag cgtgattgcc aggtctggcc caagatagac aaactcgacc
     1681 gggctcgcgt tggccggtcg ggccgaaaag cgcgcaccgt ttggcaccgt gaccgtttgc
     1741 ggtccaaccg gcgccgtgaa tgtcaggagc aagtcggcgc gcgccggaga cgaggctgag
     1801 aggtcgtagc cgatgagccg cagaaggtcg acgatgctgc gtcgttcgac agcggtcgaa
     1861 ggaaatagct cgctggcgat gcggtcctgg taatagagcg tgatgtcgcc catgtaggcg
     1921 aagaggtcga cgaacagcat gccgaggtct gccggggaac gatcggtcca ttcgggcaag
     1981 cggtcctggg cgaggcggag catcgcatcg cgcaaagaac gaaagtcctt gtcggtgtag
     2041 tcgatcgccg gtcgcggtga cagcatgccc agacctttct caggcgatcg gaacgatcat
     2101 ctcgccggcg atcggccggt cgctatgcat gtagttgagg accagcctca gctcggcttc
     2161 tcgctgctgt acgctggcgc gcgttaccac gacgcggcgc tcccattgca cgacggcctc
     2221 gtgagcctgg cgccggatca ggtccgccgt gacgtcgtcg ttgttctcgt ggaccaggga
     2281 gtggactcga gtaccgaatt cgcgcagcat ggggcgctcg ccgaggcgcg tgccgatcag
     2341 gatgcgcaag tcgtcgcgga tcttgttagc gccggtctcc caggcgatgc cacccgttcg
     2401 agaatcgata cggaaaggga atgctggacc gcttgcatct tccacttgaa gcgcctttct
     2461 agccgacagc cacggccgtt gtctgcgtgc cggtccccag cggatggcca ccgccatcga
     2521 ccacgttcac gaccacaccg ttgacgctcg cggtgacgat gtagtcgtga atcgcctgcg
     2581 cgaggtcgtt ggccacggtg tcgctgtccg gcgcgctctt accgtcggcg aagacttgcc
     2641 ggagggcgtt tttgaggtca tcgacgttca atggcacggg tacctcctca atccaaagga
     2701 catgccggaa tcgcgggcgg ctgcggcagc ggcggtggcg ccggaatcgt cgggaggccg
     2761 gggatcgcag gcagtgcggg caggatgtcc ggtatcggtg gcaaggcagg cagggttacg
     2821 gagggcagcg agagctgcgg cagcggcggc ggcgatggaa ttgccgggag gccggggatc
     2881 gcaggcagag cgggcaggat attcggtatc ggtggcagag ccggcagggt tacggagggc
     2941 agcgagagct gcggcaacgg cggtggcgac ggaattgccg gaaggccggg gatagcgggc
     3001 agcgcgggca gtatgtcggg gaatggtggg aagctgcagc ggctcattca tttcaccgcc
     3061 accgagctcg ttagatgcac gccggggatg agctgtaacg gttgcagctc gatagagggg
     3121 ccggaggggc ccatcgcggt ggggtgggtg tggagcaaga gcgccttcca gtacgtgtcg
     3181 aaggcgtttc ccatgatcgt cgggtccgag gcgcccatgc cgagcgcgac gctcttggct
     3241 tcgacgacca ccgagccagc tttgagatgc acggtgtctc ccgcgatgtt cacggtcgtc
     3301 ccgtccgggt tgacgatggc ggtgcccttg tcgttcatca cgaggtagtt accgtgctgc
     3361 tcgacgaagg tcgcctgctt gccatcggca tcgaggtgga tatgtgaacc gttcggattc
     3421 gagatgagga tgctgccttt cgaatcgatc gagatgaagg catctttcgc gtgctggatg
     3481 aggatttgct cttcgccggc cttatcgacg atctggatcg tgtgaccggc cggcgtctgg
     3541 atgacgcggc tttcgggcgg tgacacctgg gcctggaccg gcgccttgcc ctggggatac
     3601 caggcgcccg accagatggc agaggtgcgc tcgccctctt cgaactccac ccagacacga
     3661 gcgtcaacgg gcgggac
//
LOCUS       DASSGX010000380         3033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_721262, whole genome shotgun sequence.
ACCESSION   DASSGX010000380 DASSGX010000000
VERSION     DASSGX010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_721262"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            436..1341
                     /locus_tag="VFI12_06765"
     CDS             436..1341
                     /locus_tag="VFI12_06765"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HET7056147.1"
                     /translation="MEWFATNQRDLPWRQTRDPYRILVSEVMLQQTQVDRVIPYYERW
                     LELFPTAEALADAPTADVIKAWSGLGYNRRAVYLQKTARAVVDEHGGVFPRSRDELLK
                     LPGVGPYTAGAIAAFAFEQDVAFMDTNIRRVVHRLVFGSDIPARRATEQRVLEAAEHL
                     VPDGFGWAWNQAMIEFGALQCTQRRPACVICPLQRCCAAFPAIQTDIAELPVCARLKN
                     ESAFHGSNRQFRGRVVEELRRMSSATLAELGPKVKTDFAEQDLPWLYGVVSGLARDGL
                     VTIREPAAEYDAGNPASDLVVSLPG"
     gene            complement(1345..2022)
                     /locus_tag="VFI12_06770"
     CDS             complement(1345..2022)
                     /locus_tag="VFI12_06770"
                     /inference="COORDINATES: protein motif:HMM:NF022747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3105 domain-containing protein"
                     /protein_id="HET7056148.1"
                     /translation="MTEKTTTGPQTRAERRQAMVKERRNVHRQRYNRNKREMLIIKGV
                     SVALGLVILGALAFTAVNYIREQDLNRAPDGVTTYAYASANHIEGDIDYKTQPDYKGE
                     VPPAGGAHNSTAQQCEVYSQPIRQEYAIHSLEHGAVWMTYQPDLPADQVEKLKEIVGS
                     DPYLMLSPYPGLPTPIVLTAWNHQLQMPSFDKDTVERFIRSYKNKRGITPEFGASCAG
                     TTMTVGQ"
     gene            complement(2044..>3033)
                     /locus_tag="VFI12_06775"
     CDS             complement(2044..>3033)
                     /locus_tag="VFI12_06775"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF003211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HET7056149.1"
                     /translation="RTLTDARQFNLMFRTTIGPVSESGTQVYLRPETAQAIFVQYKNI
                     LATSRVKLPFGVGQIGKAFRNEITPGNFIFRDIEFEQMEIEYFVRPDQGAAKFDEWLA
                     AMEGWVRQIGLNPDRVRLREHAKDELSHYSSRTVDFEYLFPEPMGWKELYGLANRTDF
                     DLGRHQEYSGEDLRYFEQETGERFVPHVIEPTFGVDRTVLVVLLDAYDEEETVDVNGK
                     PDTRTVLRLAPVLAPYKAAVLPLMKKPELTDVARSLFGEIQRQTNFVVDYDETANIGK
                     RYRRQDEIGTPFCFTVDYESLEDKAVTVRHRDTMEQERIAIVDVQQFLRDAIR"
BASE COUNT          514 a          937 c          974 g          608 t
ORIGIN      
        1 gaggaaaagc gcgagaacag agccatagag ggtgcgccgc atccaggccg gcgagtccgc
       61 gaccgccgac cacgcgaacg ccgcgcatct gatcgccaca ccgaggacga gtgatccgcg
      121 gcgcggtttc tcggaagcga cccgccgggg atgtcgtggt gagagcgcag attgcacgga
      181 cgattccctc tgcgggacgg ttcaacgcac tattcgccga atgtacgaac aggttgtaca
      241 aacatagcga atcagtgcct ctgaggcaag ccccgtacga ggtatttctc agtggatggc
      301 ggcaaatcga tcggcgttcg tgactaatcg acacgaacac aacgtgttga tcggcgacgt
      361 gtccgatctc ccggctatgc ttcggacatg gaggagctgg acgcagaaaa catcggagaa
      421 atccggagcc acttgttgga gtggttcgcg acgaatcagc gggaccttcc ctggcgtcag
      481 actcgcgacc cgtaccgcat cctcgtctcc gaggtcatgc tccagcagac gcaggtcgac
      541 cgggtcatcc cctattacga gcgctggctg gagctctttc cgaccgccga agctctggct
      601 gatgcgccga ccgcggatgt catcaaggcg tggtctggtc tcggctacaa ccgccgcgcc
      661 gtctatctgc agaagactgc ccgcgctgtc gtcgatgagc acggcggcgt ctttccccgg
      721 tcccgggatg aactgctgaa gctgccgggc gtcgggccat acacggctgg ggcgatcgct
      781 gcatttgcgt tcgagcagga cgtcgccttc atggacacga acatccgccg ggtcgtccac
      841 cggctcgtgt tcgggagcga tatcccggcc cggcgcgcga ccgagcagcg ggtgctcgag
      901 gcggcggagc acttggtgcc ggacgggttc ggttgggcgt ggaatcaggc gatgatcgag
      961 ttcggcgcgc tccagtgcac ccagcggcgg cccgcctgcg tgatctgccc gctccagcgg
     1021 tgttgcgccg cgttcccggc gatccagacc gatatcgccg agttgccagt gtgcgcgcgc
     1081 ctcaagaatg agtccgcgtt ccacggctcg aaccggcaat ttcggggccg ggtcgtcgag
     1141 gagttgcgcc gaatgtcgag cgcgaccctg gccgagctcg gcccgaaggt gaagaccgac
     1201 tttgccgagc aggacctgcc atggctctac ggcgtggtgt ctggcttggc ccgggatggg
     1261 ctggtgacaa tcagggagcc ggctgccgag tacgacgccg gcaacccggc ttccgacctg
     1321 gtggtctcgt tgcccgggta gcgcttactg gccgaccgtc atcgtcgtgc ccgcgcagga
     1381 ggcgccgaac tccggcgtaa tgccgcgctt gttcttgtag ctgcgaatga accgctcgac
     1441 cgtgtccttg tcgaacgacg gcatctggag ctggtgattc caggcggtca ggacgatcgg
     1501 tgtcggcagg ccagggtatg ggctcaacat caggtacggg tcgctgccaa cgatctcttt
     1561 cagcttctcg acctgatcag ccggcaagtc aggttgatac gtcatccaga ctgcgccgtg
     1621 ctcgagcgag tggatcgcat actcctgccg gatcggctgg gagtacacct cgcactgctg
     1681 ggcggtgctg ttgtgcgcgc cacccgccgg cgggacctct cccttgtagt cgggctgcgt
     1741 cttgtagtcg atgtcgcctt cgatgtggtt cgcgctggca taggcatagg tcgtgacacc
     1801 gtctggcgcg cggttcaggt cctgctcgcg aatgtagttc actgcggtga acgcaagcgc
     1861 gccaaggatg accaggccga gcgcgaccga cacgcccttg atgatcaaca tttcccgctt
     1921 gttgcggttg tagcgctgcc ggtgaacgtt gcggcgctcc ttgaccatgg cctgccgccg
     1981 ctcggcgcgg gtctgtggtc cggtcgtcgt cttctcagtc atgcagggat gtcctcgatc
     2041 tctttacctg atagcgtcgc gcaggaattg ctgcacgtcg acaatcgcga ttcgctcttg
     2101 ctccattgtg tcccggtgtc ggaccgtgac cgccttgtcc tcgagcgatt cgtaatccac
     2161 cgtgaagcag aacggcgtgc cgatttcatc ctgccgccga tagcgcttcc cgatattcgc
     2221 ggtttcgtca taatcaacaa cgaaattggt ttgccgctgg atctccccga agagcgagcg
     2281 cgccacgtcc gtcagttccg gcttcttcat gagcggcagg accgccgcct tgtatggggc
     2341 cagtacggga gctaatcgca gcactgtgcg ggtgtccggc ttaccgttaa cgtcgaccgt
     2401 ctcctcttcg tcatacgcgt cgaggagcac aacgagtacc gtccggtcca caccgaatgt
     2461 cggttcgatc acgtgcggga caaaccgctc gccggtctcc tgctcgaaat agcggaggtc
     2521 ctcgccgctg tattcctggt gccggccgag atcgaaatcg gttcggttcg ccagcccgta
     2581 aagctctttc cagcccatcg gctccggaaa gaggtactcg aaatcgacgg tgcgactgga
     2641 atagtgcgag agctcatctt tcgcatgctc gcggagacgg acgcgatcgg ggttgaggcc
     2701 gatctgcctt acccagccct ccatggccgc gagccattcg tcgaacttcg ccgcgccctg
     2761 gtccgggcgg acgaagtact cgatctccat ctgctcgaac tcgatgtcac ggaagatgaa
     2821 gttgccgggc gttatctcgt tccggaacgc cttgccgatc tgtccgacgc cgaatgggag
     2881 cttgacgcgg gaggtcgcca ggatgttctt gtactggacg aagatcgcct gcgccgtctc
     2941 gggccgcagg tagacttggg taccgctctc gctgaccgga ccaatcgtcg tccggaacat
     3001 caggttgaat tggcgcgcgt cggtcagggt gcg
//
LOCUS       DASSGX010000381         2009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1157993, whole genome shotgun sequence.
ACCESSION   DASSGX010000381 DASSGX010000000
VERSION     DASSGX010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157993"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VFI12_06780"
     CDS             <1..375
                     /locus_tag="VFI12_06780"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HET7056150.1"
                     /translation="MARGIDRSRDRPPRLLSGRARPPGHLIAIVATAATLALAACADR
                     QETVALPPVPTLVVVTATAGPPPVPTPGEQQRYVVREGDTLSGIAARFGVSEDAILQE
                     NPLSDRDRLLVGQELIIPPAQP"
     gene            complement(709..1374)
                     /locus_tag="VFI12_06785"
     CDS             complement(709..1374)
                     /locus_tag="VFI12_06785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HET7056151.1"
                     /translation="MANEQRELGAERRELLGKQVSRLRRAGRIPGVVYGPVIESTVPV
                     SVDRREFLKFYQANGHSTLFVLRWEGGEESVFIREVQEDPLRREPLHIDFFAPNLRNP
                     VRAMVPIVLHNPVSTSEAVLTEARTQVEVEALPMHIPSQIDLDVSGLLRAGDLIRVGD
                     LTLPPDVTSVTDADEVVVQMEAVYRPPVEEPEVVEAPEAAIPEPQPDNVPATEASGGS
                     GED"
     gene            1780..>2009
                     /locus_tag="VFI12_06790"
     CDS             1780..>2009
                     /locus_tag="VFI12_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7056152.1"
                     /translation="MGRYLLRRALISVVTLIAISMVVYAILAIAPGDPLAGFASNPNV
                     PPELRERIRKTMGLDDPIHIQYLRWASAYIHG"
BASE COUNT          330 a          723 c          620 g          336 t
ORIGIN      
        1 atggcccgag gaatcgaccg ctctcgcgac cggccgcctc gtcttctgag cgggcgcgcg
       61 cgtcctcccg gacacctcat cgcgatcgtg gcaacagcgg cgactctggc gctcgccgcc
      121 tgtgccgacc gccaggagac agtggcgctg ccacccgtgc ccacgctggt ggtggtgacc
      181 gccaccgccg gtcctccacc ggtgccgacg ccgggcgaac agcagcgcta tgttgtacgc
      241 gaaggggaca cgctctccgg catcgccgcc cgcttcggcg tgtccgaaga cgcgatcctg
      301 caggaaaatc cgctctccga ccgcgatcgc ctgctggtcg gccaggagct catcatcccg
      361 ccggcccaac cctgacctgt gggcgggacg gacgcgaagc ttctggtcgg cgccgccatc
      421 gtcatcagtg ccgaatctgg agcctgcaca gccgaccggc caacggcccc cttgaccgcg
      481 aatcgggggc atggtaccgt tccgttgacg tcgcgagatg cggcgtgaac tccaagaccg
      541 agcagggtaa aggaggtgat gcccatgagt agtcacggta tcaagggcat cgcggtggag
      601 gctgttcgcc gctaggcgca gccgccgtga tgccatgatg acgagggccg gacatttcgt
      661 ccggccctcc gtctttctcg gcaatcgttc gggtcgcccc gcagccgctc agtcctcgcc
      721 gcttcccccg gaggcttccg ttgccggcac gttgtccggc tggggctccg ggattgccgc
      781 ttctggcgcc tcgaccacct ctggctcttc cactggcggc cggtaaaccg cctccatctg
      841 caccacgacc tcgtccgcgt cagtcaccga tgtgacatcc ggcggcaacg tcaggtcacc
      901 gacccggatg agatcgccag ctcgcagcag ccccgataca tcgaggtcga tctgcgacgg
      961 aatatgcatc ggcagcgcct cgacttcgac ctgggtgcgc gcttctgtga gcaccgcctc
     1021 cgaggtgctg accggattgt gcagcacgat cggcaccatg gctcgcaccg gattgcgcag
     1081 gttcggcgca aagaagtcga tgtgcagcgg ctcccggcgc agcgggtctt cctgcacctc
     1141 ccggatgaag actgactcct cgccaccctc ccagcgcagc acgaacaggg tggagtggcc
     1201 gttcgcctgg tagaacttga ggaactcccg gcggtcgacg ctgaccggga ctgtcgattc
     1261 gatcaccggc ccgtagacga ctccggggat gcggcccgcg cgccgcagcc ggctcacttg
     1321 cttgccgagc agctcgcgcc gctccgctcc gagttctcgt tgctcgttcg ccatctcgac
     1381 tatccgatcc tgaatccgaa cacccgccgc gaccatcggg tcacggcacg caatcacaac
     1441 aatcccggcc tggctcgcca cgagccaacc cggaagcgac cagccatcct accacaaaga
     1501 gcccgtggct ctgcccgatt cgccgcgctg gcgaaccctc ttgggagcgc caggccgcga
     1561 attaccttga cgcatcagcc cgcgagccgt accatcagcc ggactttcca tccgtctcgc
     1621 atgcccacgc gttgtcgaga caaagacacg gatcacgcga tgaaacgcct ggagagttgc
     1681 cagactcggc gcgacgtcgg cgggcgaaac cactgccgtc gcgcaacgat cggcggcgcc
     1741 gccgcctggt cgcaacgcgg ctgacaagga ggtcgccggt tgggacgcta tttgctacgc
     1801 cgggcgttga tctcggtggt caccctcatc gccatcagta tggtcgtcta cgcgatcctg
     1861 gccatcgcgc cgggtgaccc gctggctggc ttcgcctcca acccgaacgt cccgcctgag
     1921 ttgcgggagc gtattcggaa gaccatgggg ctcgacgacc cgatccacat ccagtacctg
     1981 cgctgggcgt cggcctacat ccacgggga
//
LOCUS       DASSGX010000382         2949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_68892, whole genome shotgun sequence.
ACCESSION   DASSGX010000382 DASSGX010000000
VERSION     DASSGX010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68892"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1515)
                     /locus_tag="VFI12_06795"
     CDS             complement(<1..1515)
                     /locus_tag="VFI12_06795"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7056153.1"
                     /translation="MQQMIPIFIPSFNNPTYLRGMLDQLHARGLSNIIVVDNASTLPE
                     MQALLESDLGVTVVRLDRNAGPRSIFLDDDNYALLPDLFCVTDPDLELNPGLPPDFLA
                     RLAGLTERFQIGKAGFSLDISDRESLRQETFDMGWDSRYTIWDWEQQFWVNPVGELDD
                     LGTIYRATIDTTFSVYNKRYFRRDQYMDAVRVAGMFTCRHLPWYKEATIPRLEAETYR
                     ATSQYSCYPPEEPAADEASGETDEGGGDWQETPSLGEAILGVPARDVLVIVPTRSRPE
                     NAAAFAEAFVSNSRRSDLLFGLDEDDAHNYPRLPGVMYEVNPRLRLNGTLNLIAKKYM
                     DRYEYIAFMGDDHRVRTPGWDELLVEAIVDVPHGIAYGNDLYQGANLPTAVLMKSSII
                     RRIGYMTPPQLTHLCLDDFWLDLGQSLGSIRYIDSVIIEHLHPSLGKANYDGSYLETN
                     TNEMYERDLAALARYKNERFALDIALLQGNMTRLRLRPPYSETELRQIYATPHQH"
     gene            1784..>2949
                     /locus_tag="VFI12_06800"
     CDS             1784..>2949
                     /locus_tag="VFI12_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056154.1"
                     /translation="MGASMEYVVERGMRWLPESSWRPVMQRSIDAFYEGKPDEGVAAC
                     RTLLNAVGLPDEIRNLTYQNQTYYARPLTELIPGAAWQPLPMPVPAGWVARDPSPAAR
                     DERLLVLVRAAPATEPVESGEEAGAAIPEGMEIVVLRDEDRGGPRLSEARPFFAGEAI
                     QVAAVVREPGEGVRDRAGVVTLTEGKAGEPRLLEPRPGNFRGGWSPLLTDAGPRFIGW
                     WEPTEVFRLDDATGEFARVALRMAPHLAERFQGGSQGVPVPGGYLLLVNETVTMGDGN
                     AVTLSRFVRVDEGFQITDVSPQFYLGERGRDVATGLARQGERLIAAFTANGRSALATM
                     DLAPVLDLDDRPGPSATNPDGTRSYGIDPDVAADDGVAFARGLRAAGVVPVVKH"
BASE COUNT          504 a          900 c         1031 g          514 t
ORIGIN      
        1 atgttggtgt ggcgtagcat agatttgccg aagttcggtc tcggagtacg gtggcctcaa
       61 acggaggcgg gtcatattgc cctgcagtag tgcgatatct aacgcgaaac gctcattttt
      121 gtagcgagca agcgcggcga ggtctcgctc gtacatctcg ttggtgttcg tctctaggta
      181 ggagccatca taatttgcct tcccgagcga gggatgcaaa tgttcgatga tgacgctgtc
      241 gatgtagcgg atactcccta gggactgtcc cagatcaagc cagaaatcgt cgaggcagag
      301 gtgtgtcaac tggggaggag tcatataacc aatcctacgg ataatcgaag acttcattag
      361 tacggcggta gggaggtttg ccccctggta caggtcattg ccgtaggcaa taccgtgcgg
      421 cacgtcgacg atcgcttcca caagcaactc atcccagcct ggtgtccgca cccgatggtc
      481 atcacccatg aacgcaatgt actcgtatcg atccatgtac ttcttcgcga tgagattcag
      541 cgtgccgttc aggcgcaggc gggggttcac ttcgtacatg acgcccggaa gtcgcggata
      601 gttgtgcgca tcgtcttcgt ccaagccaaa gaggagatcg gaccgccgag agttggagac
      661 gaacgcctcg gcgaaggccg cggcgttctc gggccgagag cgcgtcggca cgatcactag
      721 tacgtcgcgt gccgggacac ctaggatcgc ctcaccgagg gacggagttt cctgccaatc
      781 accccctccc tcgtccgttt caccgctcgc ctcgtccgct gccggttcct ccggcgggta
      841 gcacgaatac tgcgaggtcg cgcggtaggt ttccgcctcc agccggggta tcgtggcctc
      901 cttataccaa gggaggtgac gacaggtgaa cataccggcc acgcgcacgg cgtccatgta
      961 ctggtcacgc cggaagtacc gcttgttgta gacggagaac gtcgtatcaa tggtggcgcg
     1021 atagatcgtg cccaggtcgt cgagctcccc gactgggttg acccagaact gctgctccca
     1081 atcccagatc gtgtagcggc tgtcccagcc catgtcgaag gtttcttggc ggagcgactc
     1141 gcggtctgag atatcgaggc tgaaccccgc tttgccgatc tggaaacgct cggtcagccc
     1201 ggcgaggcga gccagaaaat ctggcggcag gccgggattc agttccaggt ccgggtctgt
     1261 gacgcagaac aagtcaggca gcagcgcgta gttgtcgtcg tcgagaaaga tgctccgcgg
     1321 cccggcgttc ctgtcgaggc gaacgacagt cacgccgaga tcgctctcca gcagggcctg
     1381 catctcgggc agcgtcgagg cgttgtcgac gacgatgatg ttggacagcc ccctcgcgtg
     1441 gagctggtcg agcatgcccc gcaggtaggt cggattgttg aacgacggga tgaaaatcgg
     1501 gatcatctgc tgcatggatg ccgccagcga cggcgatgcg gccgatgccg gcgtgcacgg
     1561 aaatgcgggt tgccaaccca ttggtcgccc ctcgaatgtt cgccccgatg ttgagaggtg
     1621 catggtaacg catctgccgc tcgaatggaa tgccgaagcg ctgagttttc ccgccattct
     1681 gcgagattcg ggagctgctg cggagtgtgt tgggttccca gggtcatacg cagggtgtac
     1741 gtcgtgcagt aagatcgccg cgaaccggaa ggggcgaaca gccgtgggag cgtcgatgga
     1801 atacgtcgtc gaacggggca tgcgctggct gccggagtcg tcctggcggc cggtgatgca
     1861 gcgctcgatc gatgccttct acgaggggaa gcccgacgag ggggtggcgg cgtgccgcac
     1921 gctcctcaac gcggtcgggc tccccgacga gatccggaac ctcacctacc agaaccagac
     1981 ctactacgcg cggccgctga cggagctgat ccccggcgcg gcatggcagc cgctgccgat
     2041 gcccgtgccg gcggggtggg tggcgcgcga cccgagcccg gccgcgcgcg acgagcggct
     2101 gctggtcctg gtgcgggcgg cgccggcgac ggagcccgtg gagagcggcg aggaggcggg
     2161 tgcggcgatc ccggagggga tggagatcgt cgtcctgcgc gatgaagacc ggggcgggcc
     2221 gcggctgagc gaggcgcgtc ccttcttcgc gggcgaggcg atccaggtgg cggcggtcgt
     2281 gcgggagccg ggggagggag tgcgcgaccg ggcgggagtg gtcacgctga cggagggcaa
     2341 agcgggcgag ccgcggctgc tggagccgag gccgggcaat ttccggggcg ggtggtcgcc
     2401 gctgctgaca gacgcagggc cgcgcttcat cggctggtgg gagccgacgg aggtcttccg
     2461 gctcgatgac gcgacgggcg aattcgcgcg ggtggcgctg cgcatggcgc cgcacctcgc
     2521 ggagcggttc cagggcgggt cgcagggcgt gccggtcccc ggcggctatc tcctcctcgt
     2581 caacgagacg gtcaccatgg gcgacggtaa cgcggtcacc ctcagccgct tcgtccgcgt
     2641 cgacgaaggg ttccagatca ccgacgtctc gccgcagttc tatctcggcg agcgcgggcg
     2701 cgacgtcgcc accgggctcg cccgccaggg agagcgactg atcgccgcct tcaccgccaa
     2761 cggccggtcg gcgctggcga cgatggacct ggcgccggtg ctcgacctcg acgaccgtcc
     2821 cgggcccagc gccaccaacc cggacggcac ccgctcctac ggcatcgacc cggacgtcgc
     2881 cgccgacgac ggcgtcgcct tcgcgcgggg cctgcgagcc gccggcgtcg taccggtcgt
     2941 caagcactt
//
LOCUS       DASSGX010000383         2285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1485821, whole genome shotgun sequence.
ACCESSION   DASSGX010000383 DASSGX010000000
VERSION     DASSGX010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1485821"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VFI12_06805"
     CDS             <1..330
                     /locus_tag="VFI12_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056155.1"
                     /translation="QWADPSAAPLRYATWRPMFDPFNLLNLVFSENGFLSRHKNPDIQ
                     ALIDQAAVETDPAKRAALYQQLGTLMFEQPAALYLWDVTALYGTAAGLNWSPRPDDAI
                     VPTFHGS"
     gene            296..946
                     /locus_tag="VFI12_06810"
     CDS             296..946
                     /locus_tag="VFI12_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET7056156.1"
                     /translation="MTPSSPRSTEADLDRPTGLDALIRGRRSVRKLLPDPVPGEVIRG
                     AIEAAGWAPSPHGRQPWRFAIIESAAVKRQLAEAMAATWREQLALDGQDQAVIGIRLR
                     KSQERITGAPVIVVACLYLADLDVYPDPDRAEAERTMAIQSLGAAVQNFLLHVYASGY
                     DAGWMCAPLFRPDVVRDTLDLPAGLIAQAMLPVGRAAADPVRRPRRPVDDLIAMWE"
     gene            951..1793
                     /locus_tag="VFI12_06815"
     CDS             951..1793
                     /locus_tag="VFI12_06815"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HET7056157.1"
                     /translation="MSAEHRMLMIFAHPDDESFGFAGTLAALHEAGVEVSLVTATRGE
                     EGQILVPELATRETLGRVREQELRDAMALVGITDIRFLGFHDSGMAGSPENANPKAFM
                     NQDVEALAATVADIIAEQRPTMVLTYGLDGVYGHPDHVTVHKVAYAAVLKAGEDGRWK
                     TPNLYYSSAPRERFRRMAELPNNPFASMDPAVVATFGTPASEITTWIDTSRWADQKVA
                     VLKAHRTQVGDDGPFKDFPDDIRRMWFSLETARNVPLPWNPQPVDVLAEILPAATHDH
                     PFRT"
     gene            complement(1961..>2285)
                     /locus_tag="VFI12_06820"
     CDS             complement(1961..>2285)
                     /locus_tag="VFI12_06820"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HET7056158.1"
                     /translation="VEKYPIITFVSREVKPLGKDKFQLIGDLTIKDITEATVINVEPT
                     GESTSPWGQQVMGFEASTNVNRKDYGLTWNVALETGGFLVGDDIKINLEVEAIQQQAE
                     AAEAA"
BASE COUNT          388 a          758 c          746 g          393 t
ORIGIN      
        1 caatgggccg atccgagcgc cgctccgctg cggtacgcga cctggcggcc gatgttcgac
       61 ccgttcaacc tgctcaacct cgtcttcagc gagaatggct tcctctcccg tcacaagaac
      121 ccggacattc aggcccttat cgatcaggcg gcggtcgaga ccgacccggc gaagcgggcc
      181 gcgctctatc agcaactcgg cacgctcatg ttcgagcaac cggccgcgtt gtacctttgg
      241 gatgtcacgg cgctgtatgg cacagccgcc ggtctgaact ggagcccgcg cccggatgac
      301 gccatcgtcc ccacgttcca cggaagctga cctggaccga ccgaccggcc tcgatgcgtt
      361 gattcgaggc cggcggtcgg tccgcaagct gctgcccgac ccggtcccgg gagaggtgat
      421 tcgtggcgcg atcgaggcgg ctggctgggc gccctccccg catggtcgcc agccgtggcg
      481 gttcgcgatc atcgagtcgg ctgccgtcaa gcggcagttg gccgaagcga tggccgccac
      541 ctggcgggag caattggcgc ttgatgggca ggaccaggcg gtaatcggca tccggctgcg
      601 caagagccag gagcggatca ccggcgcgcc ggtgatcgtc gtcgcctgcc tgtacctggc
      661 cgacctcgac gtctaccccg atccggatcg cgcggaggcg gagcgaacca tggcgatcca
      721 gagcctcggc gcggccgtgc agaacttcct gctccacgtc tatgccagcg ggtacgatgc
      781 cggatggatg tgcgccccgc tgtttcgtcc cgacgtggta cgggatacgc tcgatctgcc
      841 ggcggggtta atcgcgcagg caatgctgcc ggtcgggcgc gcggctgccg acccggtccg
      901 ccgaccgcgc cgtcctgttg atgatctgat agccatgtgg gagtagtgca ttgagtgccg
      961 agcaccggat gttgatgatc ttcgcccatc ctgacgacga atcgttcggc ttcgcgggga
     1021 cgctggcggc gctgcacgag gccggtgttg aagtctcact ggtgacggcg acacgcggcg
     1081 aagagggcca aatcctcgtg ccggagctgg ctacccggga gacgctcggc cgggtccggg
     1141 agcaggagtt gcgcgacgcg atggcgctcg tcggcataac cgatatccgc ttcctcggct
     1201 ttcacgactc aggcatggcc ggctcgccgg aaaacgcgaa cccgaaggcg ttcatgaacc
     1261 aggacgttga agcgttggcg gcgacggtgg ccgacatcat cgccgagcag cgaccgacca
     1321 tggtcctgac ctacgggctg gacggcgtct atggacatcc cgaccatgtc acagtccaca
     1381 aggtcgccta tgccgccgtg ctcaaggcgg gtgaagatgg acggtggaag acgccgaacc
     1441 tgtactactc atcggcgcca cgagagcgat tccggcggat ggccgaactg cccaataacc
     1501 ccttcgcatc gatggatccg gccgtggtgg ccaccttcgg cacgccggcg agcgaaatca
     1561 cgacctggat cgacaccagc cgctgggccg accaaaaggt ggccgtcctg aaggcgcatc
     1621 ggacccaggt cggtgacgac gggccattca aggatttccc ggacgatatc cgccggatgt
     1681 ggttctcgct cgagacggcg cggaatgtgc cgctcccctg gaatccccag ccggtcgacg
     1741 tgctcgccga gatcctgccc gccgccacgc acgatcaccc cttccggaca tagcgcggct
     1801 caagaaacgt cgccccgggc ggctcgcccg gcttcagccc acggcggccg ggcgggctgc
     1861 ccgggctgca tcgcgacaag agcgatcgcc gcatacagca caaacaccaa aacggcggct
     1921 ggaattccag ccgccgtttt ggttgagaaa ggagggaagc ctatgcggcc tcggcggcct
     1981 cagcctgctg ctgaatcgcc tcgacctcga ggttgatctt gatatcgtca ccaacgagga
     2041 acccgcccgt ttcgagcgcc acgttccagg tcagaccgta gtccttgcgg ttgacgttgg
     2101 tgctcgcctc gaagcccatc acctgctggc cccacggcga cgtgctctca ccggtcggct
     2161 cgacgttgat cacggttgcc tcggtgatgt ccttgatggt caggtcgccg atgagctgga
     2221 acttgtcctt gccgagcggc ttgacctcgc ggctcacgaa cgtgatgatc gggtatttct
     2281 ccacc
//
LOCUS       DASSGX010000384         3982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_285872, whole genome shotgun sequence.
ACCESSION   DASSGX010000384 DASSGX010000000
VERSION     DASSGX010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3982
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285872"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="VFI12_06825"
     CDS             complement(<1..757)
                     /locus_tag="VFI12_06825"
                     /inference="COORDINATES: protein motif:HMM:NF026042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HET7056159.1"
                     /translation="MDAAEVDALTGAMASYAIAGQGFADIEVDYTRRIRLLAFVDASF
                     YSNFIERAGPRLPIYTGDAQWFRDNIPCMTACPSHTDISRYIALIADGRYADSFELNR
                     ENNVFPGCLGRMCARPCEDACRRKEIDAPIGICYLKRVASDYRGETRREVPQPWNGLT
                     AAVIGAGANGLTGSRELARRGYKVTIYERYPVPGGVMWAGVPEWRLPRDVIMDEVSLI
                     TDLGIEIKYNTEVGRDISLKELADTHDVVLISGG"
     gene            1143..1478
                     /locus_tag="VFI12_06830"
     CDS             1143..1478
                     /locus_tag="VFI12_06830"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HET7056160.1"
                     /translation="MDEQPTGNRDEDALAEIIRRIVRVAAPERVVLFGSAARGEAGPD
                     SDLDLLVIKPGRYHRGRLTDEIYRSLIGVGQAVDVVVVTPEDVERYRDALGLVIAPAL
                     REGRVVYAA"
     gene            1468..1830
                     /locus_tag="VFI12_06835"
     CDS             1468..1830
                     /locus_tag="VFI12_06835"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HET7056161.1"
                     /translation="MPPERRPPDDPCEWLNRARSNLVRARVTLPGVYLEDLCFDAQQA
                     AEKAIKAVLIARGVSFPLVHDLARLLAVLGQSGETIPPDIADATDLTRFAASTGYPGV
                     AEPVTAAEHRRAVTIADA"
     gene            complement(1931..2926)
                     /locus_tag="VFI12_06840"
     CDS             complement(1931..2926)
                     /locus_tag="VFI12_06840"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HET7056162.1"
                     /translation="MVATTWTVAVTRRIPEAGIRPLRDVAEVRIWEEELPPSPDQLVA
                     LLADCDGAITLLTDQIDGALLDRVPSLRVVSNFAVGYDNIDVAAGTERGVLVCNTPGV
                     LTEATADFTWALLLAAARRIPESVDYVHEGRWQTWGPTLLLGQDVSGATLGVVGLGRI
                     GKEVALRAGGFGMRVLAYDPVPDEVFAKEHGVAYVPLDDLLDESDFVTLHVALTPETE
                     HLIGTPELARMKPTAVLVNASRGPVVDTEALVAALEDGEIFAAALDVTDPEPLPADHP
                     LVSMPNCIVVPHTASATVQARDRMARLAADNLLAGLRGERPPASVNADEVLGANR"
     gene            complement(3254..3556)
                     /locus_tag="VFI12_06845"
     CDS             complement(3254..3556)
                     /locus_tag="VFI12_06845"
                     /inference="COORDINATES: protein motif:HMM:NF015630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HET7056163.1"
                     /translation="MATIQLDLDEDLLILLRRQDPSPSIEVAARELMVMELYRRGAVS
                     RGKSAELLGIPLVEFLQRANELGIPYFDYTEEEWAGQMRQVEREVASHPSSPTRAH"
     gene            3707..>3982
                     /locus_tag="VFI12_06850"
     CDS             3707..>3982
                     /locus_tag="VFI12_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HET7056164.1"
                     /translation="MTGKRFRNTTQRAEVLEVVRCAETHLTAGEIFERVRRRDPRIAY
                     GTVYRTLHLLAEHGLIQELTFADQASRYDARVERHDHVHCLHCGILLD"
BASE COUNT          707 a         1257 c         1346 g          672 t
ORIGIN      
        1 agccgccgga gatgaggacg acgtcatggg tgtccgcgag ctctttgagc gagatatcgc
       61 gcccgacctc ggtgttgtac ttgatctcga taccgagatc ggtgatcagg ctcacctcgt
      121 ccatgatgac gtcgcgcggc aggcgccact cggggacgcc ggcccacatg acgccgccgg
      181 ggacggggta gcgctcgtag atggtgacct tgtagccgcg gcgggccaac tcgcgcgagc
      241 ctgtcaggcc attggcgccg gcgccgatga cggcggccgt cagcccgttc catggctgag
      301 gcacttcgcg ccgggtctcg ccacggtagt ccgacgccac ccgcttcaga tagcagatac
      361 cgatcggggc gtcgatctcc ttgcgccggc aggcgtcctc acagggacgc gcgcacatgc
      421 gcccgaggca tcctgggaag acattgttct cgcggttgag ctcgaaggag tcggcgtagc
      481 ggccatcggc aatgagggcg atatagcggg agatgtcggt gtgcgagggg caggccgtca
      541 tgcaggggat attgtcccgg aaccactgcg cgtcgccggt gtagatcggc aggcgaggac
      601 cagcgcgctc gatgaagttc gaatagaacg aggcgtcgac gaacgccagc agccggatgc
      661 gccgcgtgta gtcgacctcg atatcggcaa acccctggcc ggcgatcgcg tacgacgcca
      721 tggcgccggt gagcgcatcg acctccgccg cgtccatggg acggaagcga tcggcgagcg
      781 cgccacagga gacacagaca tccgggggcg cctcgccctc gtgaacccag ccgcagttct
      841 tgcatcggaa catgagcgcc actttccagc tcaagcaacc tcgacagtgc gggccggccc
      901 gcgtttgcgc cgaccgtgcg ctgggagtat acccgtggcg cgccgcagat tcgcgccgct
      961 ccccactaat cgctgccata atgtctcaaa tggagggcga ggaagcgcaa ccgatcgccc
     1021 gcactgtccg cgtcaactgc ccgcgtcaat cgtatttagc gacgggtccc gcaggtcaat
     1081 tgaatccttt gcggaaacat tcccgcggcc gaacccgtcg cttatgccaa caggtgagcc
     1141 tgatggacga gcaaccaacc ggcaatcgcg acgaagatgc gctggccgag atcattcgcc
     1201 gcatcgtccg ggttgccgcg ccggaacgcg tcgtcctttt cggctctgcc gcccgtggcg
     1261 aggctgggcc ggacagcgac ctcgacctgc tggtcatcaa gcccggtcgt taccaccgcg
     1321 gacgtctgac cgacgagata taccgaagcc tgattggcgt cggccaggcg gtggacgtgg
     1381 tcgtggtcac accggaagat gtcgagcgct accgagacgc ccttgggctg gtgatcgccc
     1441 cggcgctgcg ggagggccgg gtcgtctatg ccgcctgaac gtcggccgcc agacgatccg
     1501 tgcgagtggc tcaaccgcgc ccgcagcaac ctggtgcgag cacgggtgac gctgcccggc
     1561 gtctacctgg aggatctctg tttcgatgcc cagcaagcgg cggagaaggc catcaaggcc
     1621 gtcctgatcg cgcgcggcgt ctcctttccc ctcgtccacg acctggcccg tctgttggcc
     1681 gtcctcgggc agagcggcga gacgattcct ccggatatcg cggacgcaac cgatctgacg
     1741 cgttttgcgg ccagcaccgg gtacccgggc gtcgcagagc cggtcactgc cgctgagcac
     1801 cgacgggccg tgacgatcgc cgatgcgtaa cccaccatcc gcggtccgta tcagaccggc
     1861 gggcggatcg ccgggcctga agcgccatgc aaagcttgtg tcatgtcggg cggctaacag
     1921 cgaacgagac ctacctgttg gcgccgagta cctcatcggc gttgaccgag gcgggcggtc
     1981 gctcgccgcg caagccggcc aggaggttgt cggcggcgag tcgcgccatg cggtccctgg
     2041 cctggaccgt ggccgaggca gtgtgcggga cgacgatgca gtttggcatg ctgacgagcg
     2101 ggtggtcggc tggcagtggc tcggggtccg tgacgtcaag tgccgcggcg aaaatttctc
     2161 cgtcttcgag ggcggcgacg agtgcctcgg tgtcgacgac cggaccgcgg gaggcattaa
     2221 ccaggaccgc ggtcggtttc atgcgggcta gctctggtgt gccgatcagg tgctctgtct
     2281 ctggcgtgag ggcgacatgg agggtgacga agtcggactc gtccagcaga tcgtcgagcg
     2341 ggacgtaggc aacgccgtgc tctttggcga acacctcgtc cgggaccggg tcgtacgcga
     2401 gaacgcgcat gccgaaaccg ccggcccgca aggcgacttc cttcccgatg cgcccgagac
     2461 cgacgacccc gagcgtggcg ccgctgacgt cctgaccgag caggagcgtc ggtccccacg
     2521 tttgccaccg cccctcatgg acgtaatcga ccgattccgg gatgcgccgc gcggcggcga
     2581 ggaggagggc ccaggtgaaa tcggcggtcg cctcggtgag gacgccgggg gtgttgcaga
     2641 cgagcacgcc gcgctcggtg ccggccgcga catcgatatt gtcgtagccg accgcgaagt
     2701 tcgagacgac gcgcagggat ggaacacggt cgagcaacgc gccgtcgatc tgatcggtga
     2761 ggagcgtgat agcgccgtcg cagtcggcga gcaacgcgac gagttggtcc gggctgggcg
     2821 ggagctcctc ctcccagatg cggacttccg cgacatcccg cagtgggcgg atgcccgcct
     2881 ccggaatgcg ccgggtcacc gccacggtcc aggtcgtcgc caccacgttc cctcctatct
     2941 gcgtgccaat gacttccagc cgtctgggca acctcattgt cgccggattc gcgagattgt
     3001 gggaatggcg atgcgtttgc ctcggctagc gcaattccca ccctattggc taggttgcgc
     3061 atggtggcta agactcattc tcaactacag cacgcgatgt gatgaccggg aagcgattca
     3121 ggaactcgac gcagcgccgc ggtgccggag gagattcgct gctcgccgga tcgtcaccgg
     3181 atcgtcatgg gaacgacgac ttcgccgaaa gcgccagcca acaaatcaat tcggctgatg
     3241 cgatcgagag cgattaatgg gctcgcgttg gagacgacgg gtgagacgcg acttcccgct
     3301 cgacctgccg catctgcccg gcccattctt cctccgtata gtcgaagtag gggatgccaa
     3361 gctcgttggc gcgctgcaga aactcgacaa gcgggatgcc gagcagctcc gccgacttgc
     3421 cgcgggagac ggcgccgcgc cgatacagct ccatcaccat caattcgcga gcggcgacct
     3481 cgatagaagg agaaggatcc tgccggcgca agaggatcag aaggtcctcg tcgagatcga
     3541 gctggatggt ggccatttct gctgctccgg agcggcttgt ggcaaatgaa cggacggggg
     3601 aatcgtagcc gagaaatcac taccctgacg tagcgaattt ccgaccagta tggtatgatt
     3661 tcctcaggtt agtgagactc attctcaact ctatctggtt gaggtcatga ctgggaagcg
     3721 gttccgcaac acaacccaga gggcagaggt gctggaggtg gtccggtgtg ccgagacgca
     3781 tctcaccgcc ggtgagatct tcgagcgggt gcgcagacgc gatccgagga tcgcgtacgg
     3841 gacggtctac cgcaccctcc atctgctggc cgagcatggg ttgatccagg aactgacctt
     3901 cgcggatcag gccagccggt acgacgctcg ggtcgagcgg cacgaccacg tgcactgcct
     3961 gcactgcggc attctgctgg ac
//
LOCUS       DASSGX010000385         2272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1486538, whole genome shotgun sequence.
ACCESSION   DASSGX010000385 DASSGX010000000
VERSION     DASSGX010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1486538"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..984)
                     /locus_tag="VFI12_06855"
     CDS             complement(<1..984)
                     /locus_tag="VFI12_06855"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7056165.1"
                     /translation="MLPSSGVARHAPVYSRRRLLQSIGAAIPAFTPIAAARQGTSSTF
                     TLVTNRTSSDLDPHSAYDPGSGVVLQGPFEGLIRLKPGSVNAYEPVLAESWSANRDGS
                     VWSFRLREGVTFHDGSPLDAEAARVSFERLFRLGLAPSTVLGRFIADPTQVVAQDART
                     LVFDLGQPQPLFEAAVAAPYGTSVVNVAALLQHEVDGDLGHSWAQTNSEGLGTGPYRI
                     IEFDTENGVVLERYDGYWGERKAGHFDRVLIRIVVEPQTRRALLENGDADLATTLPLA
                     AIRDLEQHPELTVDRRYNLAVNYLAMTVSGPLQSPEARQAMCWAFPYREVID"
     gene            1179..>2272
                     /locus_tag="VFI12_06860"
     CDS             1179..>2272
                     /locus_tag="VFI12_06860"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET7056166.1"
                     /translation="MRDPKFARAAARSTLSRRTLLGAATAAALGALSTRGSAAAAPGP
                     WGFEIAVTRAHSAALADDPLPSWNDGPRKQAILDFVAAATDQATTDFLPLPDRIATFD
                     MDGTLWVEMPFYTENAFLVARLQALSADHPDWRTTPPFAKVLTSGGEALLTLNDQDWE
                     AIMAASQVGLTMEEYANTVATWIAAAKHPRFDRLYTDLIYQPMLEVMAYLRDNDFRTY
                     IVSGSGQEFMRTYADQVFGVAPEQVIGTTFQVSYEMGNDGAPVIRIDAAPLTDDNFAG
                     KPEDIALFIGRRPAAAFGNSTGDQQMLEWAGAGERATLMMLVYHDDAAREYAYGPAGG
                     LPDTHVGTFTDALMTEAKDRGWSVISMKDD"
BASE COUNT          410 a          769 c          750 g          343 t
ORIGIN      
        1 gtcgatcacc tcccggtagg ggaaggccca gcacatcgcc tgtcgcgctt cggggctttg
       61 caggggaccg gagacggtca tggccaggta attgacagca aggttgtagc gtcggtcgac
      121 ggtcagctcc gggtgctgtt ccagatcgcg gatcgccgcc aggggcaagg tcgtggcgag
      181 gtcggcgtcg ccgttctcga gcagggcgcg gcgcgtctgc ggctcgacga caatgcggat
      241 caagacgcgg tcgaagtggc cggctttccg ttcaccccag tacccgtcgt agcgttcaag
      301 gaccacgccg ttctcggtgt cgaactcgat gatgcggtac ggaccagtgc cgagaccctc
      361 gctgttggtc tgcgcccacg agtggccgag gtcgccatcg acttcgtgct ggagcagcgc
      421 ggcaacattg acaaccgacg tgccgtacgg cgcggcaacg gcggcctcga agagcggttg
      481 cggctggccc aggtcgaaga cgagcgtgcg cgcgtcttgc gccacgacct gggtcggatc
      541 ggcgatgaag cggccaagga ccgttgacgg ggcaagcccc aggcggaata ggcgctcgaa
      601 cgagacccgc gccgcttcgg cgtcgagcgg agatccgtcg tgaaacgtta ctccttcgcg
      661 caggcggaaa gaccagacgc tgccgtcgcg attggccgac cacgactcgg ccaggaccgg
      721 ttcataggcg ttgacgctgc ccggcttgag gcgaatcagc ccctcgaagg gcccctgcag
      781 gacgacgccc gagccagggt cgtacgccga gtggggatcg aggtcggatg acgtccggtt
      841 ggtgaccagg gtaaaggtgg atgacgtgcc ttgccgagcg gcggcaatcg gggtaaatgc
      901 cggaatcgcg gcgccgatgc tctgcagcag gcgccgtcgc gagtacaccg gagcatgtct
      961 tgccactccc gatgacggaa gcataatcct cctcgcatct tgccaactca aatgcggtcc
     1021 aatgaacaca gatcatgagg ccattgcgcc gaacagtcaa ccggtcgccg cagccgccgg
     1081 gtcccggcgc ttgacctgac gtcaacaatc ggatcaaatg cgcgcaacac atcaaccgag
     1141 aagcccggac ctcacctgcg ccggagggaa cgaccatcat gcgcgacccg aagttcgcac
     1201 gcgccgcggc gcggagcacc ctcagccgcc gcacgctcct cggcgccgcc accgccgcgg
     1261 cgctgggtgc gctatccacc cgcgggagcg ccgccgcggc tccggggcca tggggtttcg
     1321 agatcgccgt caccagggcc cactcagcgg cgctcgcgga tgatccgctc ccttcctgga
     1381 acgatggtcc caggaagcag gcgatcctcg atttcgtcgc cgcggccacg gatcaggcga
     1441 ccacagactt ccttccgctg ccggaccgca tcgcgacgtt cgacatggac ggcaccctct
     1501 gggtcgaaat gccgttctac accgagaacg ccttcctcgt cgcccgcctg caagcgctct
     1561 cggccgacca cccggattgg cgcacaaccc caccctttgc caaggttctc accagcggcg
     1621 gcgaggcgct cctgacgctc aacgaccagg actgggaggc gatcatggcc gcatcgcagg
     1681 tcggcctgac gatggaggaa tacgcgaaca cggtcgcgac gtggatcgcc gcggcgaagc
     1741 acccgcggtt cgaccggctc tacaccgacc tgatctacca gccgatgctg gaggtgatgg
     1801 cctatctgcg cgacaacgac ttccgcacct acatcgtctc cggcagcggt caggagttca
     1861 tgcgcaccta cgccgaccaa gtcttcggcg tcgcgccgga gcaggtcatc ggcaccacct
     1921 tccaggtgtc gtacgagatg gggaacgacg gcgccccggt gatccgcatc gacgccgcgc
     1981 cgctcacgga cgacaacttc gccggcaagc cggaggacat cgccctgttc atcggccgcc
     2041 gcccggccgc ggcgttcggc aactcaaccg gcgatcagca gatgctggag tgggcgggcg
     2101 cgggcgagcg cgccacgctg atgatgctcg tctaccacga cgacgccgcg cgcgaatacg
     2161 cctacggccc cgccggagga ctgccggaca cgcacgtcgg gacctttacc gatgccctga
     2221 tgacggaggc caaggatcgc ggctggtcgg tcatcagcat gaaggacgat tg
//
LOCUS       DASSGX010000386         3512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_69681, whole genome shotgun sequence.
ACCESSION   DASSGX010000386 DASSGX010000000
VERSION     DASSGX010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69681"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1469)
                     /locus_tag="VFI12_06865"
     CDS             complement(<1..1469)
                     /locus_tag="VFI12_06865"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET7056167.1"
                     /translation="MTTEPHPLLLGQSTLLEILHAGLPHGVAILDANLRLVFANARWS
                     AFAGQATGAADPLPGTPFADLIPDIEGLHAAARQSIATGRPHDCPGLPLASDAGESFW
                     DLTLGPLSDGNRVVGMKVIAHEATERVLASRAAAAGARLAAFRAGVSQALASSDDIGA
                     VLQSCAEAMVRHAHAAFARIWELDEADDAYHLRASAGLYTRLDGSYSRIPAEWIRQKT
                     FGGHSPAIAIDILKTHHVREPEWAAENGLVAWANQPLVVRGQIIGFISMFARQNFDQD
                     TLEELGTVADAIAQFIERKRTERAALDGLEERVAERTRELSLLLDISRNVASTLELRP
                     LLELILEQLQTVIPYTGTALLTVEDGDLIFAGQHGPLPEDAAHRIRYPIPLMAPVWNR
                     LKAGEAIIIPDIRGETAEADVFRSVVGADLDEQLAFIGSCLWAPLVVRDELIGLLSIA
                     NRTIDAFTPRHAELATAIARQAAVAIENARLFEQVRGLAA"
     gene            complement(1566..3275)
                     /locus_tag="VFI12_06870"
     CDS             complement(1566..3275)
                     /locus_tag="VFI12_06870"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7056168.1"
                     /translation="MDARNDSPDSRPILLVVDADPDARERLGEALCRRFGADYRVLAA
                     DSAAAGLATLDRLANDGAEVALVAADHTLPGTDGIAFLALAQARHPSASRALLVGMDR
                     RGTRIPFSALESLQRATALGRIDFWVVKGWQSPEELLYPQVQEALSQWTRANRPRHEV
                     VRVIGERWSAQCHTVLDTLSRNTVPFGFYEAVSLAGQEMMGEYDVDPAQLPAAIFHDG
                     TVLRNPSGTDLAAALGVQTEPTSELFDLAIVGGGPAGLAAAVYGASEGLRTVVIEQQA
                     IGGQAGTSSMIRNYLGFPRGLSGGELTFRAFEQALLFGTQFIFSHSATGLKRNDGQPV
                     ITLADGAEVRASAAIIAAGVSYRRLGIPTLDRLIGRGVFYGAAGVEATAVAGEQVCVI
                     GGANSAGQAALHLAKFAAKVTLLVRGDSLEAGMSDYLIRQLQASPNIAVRLRTQVVDG
                     QGTDRLEALVLENGEAGTSEDVPAAAVFILIGAEPRTEWLDGAVARDERGFVMTGRDL
                     CPEPWSLQRQPLPFETSQPGVFAVGDVRHGAVMRVASAVGEGSVAVGSVHRYLAETCR
                     HGS"
     gene            complement(3316..>3512)
                     /locus_tag="VFI12_06875"
     CDS             complement(3316..>3512)
                     /locus_tag="VFI12_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056169.1"
                     /translation="IQDQGISPVPGLYFIGRSWQRSRGSALLTGVGVDAAFIVEQLDR
                     AAEPGIEVARLQPAPGAAGM"
BASE COUNT          572 a         1197 c         1176 g          567 t
ORIGIN      
        1 gcggccaaac cccggacctg ctcgaacaag cgcgcgttct cgatcgccac cgctgcttgc
       61 cgcgcgatcg cggtcgctaa ctcggcatgc cgcggcgtga aagcgtcgat cgtccggttg
      121 gcgatgctca gcaggccgat cagctcatct ctgaccacga gcggcgccca gaggcatgag
      181 ccgatgaagg cgagttgctc atccaggtcc gcgccgacga cagaccgaaa cacgtcggcc
      241 tccgccgtct cgccccggat gtccgggatg atgattgcct ccccggcctt aagtcggttc
      301 caaaccggcg ccatcagcgg gattggatag cggatgcggt gcgcggcgtc ctccgggagt
      361 ggaccgtgct gcccggcaaa gatcagatcg ccgtcctcga cggtcaggag cgccgtgccg
      421 gtgtaaggaa tgaccgtttg cagctgttcg agaatcagtt ccagcagcgg tcgtaactcg
      481 agcgtagacg cgacatttcg gctgatgtcg agcagcaacg aaagctcgcg agtccgctct
      541 gcgacgcgtt cctcgagccc gtcgagagcg gctcgctcgg tgcgcttccg ctcgatgaat
      601 tgcgcgattg cgtcggcgac cgttccgagc tcttcgaggg tgtcctgatc gaagttctgc
      661 cgcgcgaaca tggaaatgaa gccgatgatc tgcccgcgga cgaccagggg ttgattggcc
      721 catgcgacca gcccgttctc ggccgcccat tcgggttcgc ggacatggtg cgtcttcagt
      781 atgtcgattg cgatggccgg tgagtgcccc ccgaacgtct tttgccgaat ccactcggcc
      841 gggatacgcg agtacgaacc atccagccgc gtgtagagcc cggcgctggc gcgcagatgg
      901 taggcatcat ccgcctcatc cagctcccag atcctggcga aggccgcgtg cgcgtgccgg
      961 accatcgcct cggcgcatga ctgcagaacc gcgccgatgt cgtccgagct ggccaatgcc
     1021 tggctgacac ccgcccggaa tgctgccagt cgcgctccgg ccgccgctgc ccggctcgcc
     1081 aggacgcgct ccgtcgcctc gtgggcgatg actttcatgc cgacgacccg gttgccgtcg
     1141 gagagtggtc cgagcgtgag gtcccagaac gattcgccgg cgtcggaggc gagcggcaag
     1201 cctggacagt cgtgcggccg accagtggcg atactctgcc gggcagccgc atggagcccc
     1261 tcgatatcgg ggatcagatc agcaaacggc gtgccgggca ggggatcggc cgcgccggtt
     1321 gcctgccccg cgaacgccga ccaccgggcg ttggcgaaga ccagccgcag attggcatcg
     1381 aggatcgcga ccccgtgcgg gagtcccgcg tgcaggatct cgagcagagt ggattggccc
     1441 agcaggagcg ggtgcggttc ggtggtcatc gtcgaagtgc cagatatcca aaatcgccgc
     1501 tggttgatcg ccagcggcgg ttgatcaggg gcaatgcatc gtttcgatgc ctcattttag
     1561 caggctcacg acccgtgtcg acaggtctcc gccaggtacc ggtgtacgga accgacagcc
     1621 accgacccct caccgacggc cgaagcgacg cgcatgaccg cgccgtggcg aacgtccccg
     1681 accgcgaaaa cgcctggctg gctggtctcg aacggtaatg gttgccgctg cagcgaccag
     1741 ggctctgggc agagatcgcg accagtcatg acaaatcccc gttcgtcccg cgcgactgca
     1801 ccatccagcc actcggtccg gggctcggcc ccgatcagga tgaagaccgc cgccgccggt
     1861 acgtcctcgc tcgtgcctgc ctcaccattt tcgagcacga gcgcctccag ccggtccgtc
     1921 ccctgtccgt cgacgacctg cgtgcgcagc cggaccgcta tgtttgggct cgcctggagc
     1981 tgcctgatca ggtagtccga catgccggcc tcgagcgaat cgccgcgcac gagcagggtc
     2041 accttcgccg cgaacttcgc gagatgcagc gccgcctgtc cggctgagtt cgccccgccg
     2101 atcacgcaga cctgttcgcc cgcaactgcc gtcgcttcga ccccggccgc gccgtagaag
     2161 acgccgcgcc cgatcaggcg gtccagcgtc gggatgccga gccggcggta cgagacgccg
     2221 gcggcgatga tcgcggcgct cgcccgtacc tcggcaccgt ccgccagggt gatcaccggc
     2281 tgaccgtcat tcctcttgag gccggtggcg ctgtgggaga agatgaactg cgtgccgaag
     2341 agcagggctt gctcgaaggc ccggaaggtc agctcgccgc cactcaaccc gcgcgggaag
     2401 ccgaggtagt tccggatcat cgagcttgtc ccggcctgtc caccgatcgc ctgctgctcg
     2461 atcacgaccg tccggagccc ctccgatgct ccgtagacag ccgcggccaa cccggccggt
     2521 ccgccaccga cgattgcgag gtcgaacagc tcggacgtgg gctccgtctg gacgccgagt
     2581 gccgccgcga ggtcggtccc ggaggggttc cgaagcacgg tgccatcgtg aaagatcgcc
     2641 gccggcaatt gcgccggatc gacgtcgtat tcccccatca tctcctgacc ggcaagggaa
     2701 actgcctcat agaagccgaa cgggacggtg tttcgggaga gcgtgtcgag cacggtgtga
     2761 cattgcgccg accagcgctc gccgatcacg cggaccacct cgtggcgcgg ccggttggcg
     2821 cgggtccact ggctgagggc ctcctgcacc tgcggataga gcagctcttc cggtgattgc
     2881 cagcccttca ccacccagaa gtcgatccgg ccgagggcag tcgcccgctg cagcgattcc
     2941 agggcggaga aggggatccg cgtgccgcgc cggtccatgc cgaccagcaa tgcccggctc
     3001 gccgatgggt gccgggcttg ggctagcgcg aggaacgcga ttccgtcggt cccgggcagg
     3061 gtgtggtcgg cggcgacgag ggcgacctcc gccccgtcat tggcgagccg gtcgagcgtc
     3121 gccagaccgg cggcggcgga atcggcggcc aatacccggt aatcggcccc gaagcggcgg
     3181 cagagcgcct cgccgagtcg ttcccgggcg tcgggatcgg cgtcgaccac gagcaggatg
     3241 ggcctgctgt cgggggaatc gtttcgcgcg tccatggtcc gttccttcaa taccgagcct
     3301 ggcgagccgc ccgggctaca ttcccgctgc acctggcgca ggctggagcc gggcgacttc
     3361 gatgcccggc tccgcagcgc gatcaagttg ctcgacgata aaggcggcat cgaccccgac
     3421 gccggtcagc agggcggagc cccggctgcg ctgccagctc cggccgatga agtagagccc
     3481 cgggaccggt gagattcctt gatcctggat ga
//
LOCUS       DASSGX010000387         2301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1158951, whole genome shotgun sequence.
ACCESSION   DASSGX010000387 DASSGX010000000
VERSION     DASSGX010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158951"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="VFI12_06880"
     CDS             complement(<1..851)
                     /locus_tag="VFI12_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HET7056170.1"
                     /translation="MTQSISHDPQSLAGGALLDWFVTRLRAEIDQRGYRLIGQDEQTD
                     EGGIVLYPVGPEGPKSFRRRNRAVFVVGIDSAESYPEEPLKVGYPMLLRTLANLFIMI
                     CEKGRQGSPEAYFFTLEQGYYAIPYRGNQDEFISQVVDRLVPLATSQLIIENDFVTDL
                     PEALWKGDEITASITRAGKHLDDLDLLPSPFPLEDYFSPEDFRHVKSLFQMGGLSYGN
                     LSARKDETRFWMSASGVDKSKLTEVGRDILLVTDYIPERRAMRLSVPPNIQPRRVSVD
                     AIEHWMI"
     gene            1007..1873
                     /locus_tag="VFI12_06885"
     CDS             1007..1873
                     /locus_tag="VFI12_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HET7056171.1"
                     /translation="MEPFVSGPQHVAIAAIQFNPGDDKAANIEKALSLIDQAASSGAR
                     LVMLPEIWTYMGDPFRNRDNAEPIPGDLTARLADRARRHGIYLHAGTFAELVVGDERV
                     RNTAVVFDPDGEMIAIYRKIHMFDVTLDGVASYKESETVAPGEEIVTFELDGVTVGLA
                     TCYDLRFPEIFRILALRGAEVILLPAAFTMTTGKDHWEVLIRARAIENQVYLVAAGQF
                     GPNLPGKWCYGRSMIVDPWGTVLATAPDTESVITAVVDRNHLQSIRRQIPSLANRQPE
                     TYRWPDEVLVAR"
     gene            complement(1980..>2301)
                     /locus_tag="VFI12_06890"
     CDS             complement(1980..>2301)
                     /locus_tag="VFI12_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056172.1"
                     /translation="MEGPHCYEQDNCDQTGLTMPFFSYTHQEGGCSITGGYVYRGKAI
                     PSLAGAYLAGDYCSGLVWAVNPETGKASAPVETNLSISSFAEDAEGELYLIDLNGAIY
                     KIVP"
BASE COUNT          405 a          714 c          772 g          410 t
ORIGIN      
        1 tagatcatcc aatgctcgat cgcgtcgacc gagacccggc gcggctgaat gtttggcggc
       61 acgctcaggc gcatcgcccg gcgctccggg atgtagtcgg tcacgagcag gatgtcgcgg
      121 ccgacctcgg tcaacttgct cttgtcgacg ccgctggcgc tcatccagaa ccgggtctcg
      181 tctttgcggg cgctcagatt gccgtaggaa aggcccccca tctggaagag gctcttgacg
      241 tggcggaagt cctcgggcga gaagtagtcc tcgagcggga agggcgaggg cagcagatcg
      301 aggtcatcga gatgcttgcc ggcccgggtg atcgacgcgg tgatctcatc gcccttccag
      361 agcgcctccg gcaggtcggt gacgaagtcg ttttcgatga tgagctgcga ggtcgccagc
      421 ggcaccaggc gatcgacaac ctgcgagatg aactcatcct ggttgccgcg gtacgggatc
      481 gcgtagtagc cctgctccag cgtgaaaaag taggcctccg ggctgccctg ccggcccttc
      541 tcgcaaatca tgatgaagag gttggccagc gtgcggagca gcatcgggta gccgaccttg
      601 agcggctcct ccgggtagct ctcggccgaa tcgatgccga ccacgaagac cgcgcggttc
      661 ctgcggcgga aactcttcgg cccctccgga ccgaccgggt agaggacgat cccgccctca
      721 tcggtctgct catcctgacc gatcagccgg tagccgcgct ggtcgatctc cgcgcgaagt
      781 ctggtcacaa accagtccag cagcgcgccg cccgcaagac tctggggatc atgcgatatg
      841 gattgagtca tacggccctc ctgaggtggt ccgggtcaat ggttctgttc ggtaagtatg
      901 ccctgtgcgc tgcccgggca tcgattgatg ccccagaggc gcgaattggc ccctacagta
      961 agatagccgc agagggaata gaccgcaggt ggaggaggta cgggcaatgg agccctttgt
     1021 cagtggaccg caacacgtgg cgattgccgc gattcagttc aaccccggcg acgacaaggc
     1081 cgcgaacatc gagaaggctc tgagcctgat cgatcaggca gcgagcagtg gcgcgcggct
     1141 ggtcatgctg cccgagatct ggacctatat gggcgatccg ttccggaacc gggacaacgc
     1201 ggagccgatc cccggcgacc tgaccgcgcg gttggccgac cgagcccggc ggcacggcat
     1261 ctatctccac gccggcacct tcgccgaact ggtggtggga gacgagcggg tccggaacac
     1321 cgccgtcgtt ttcgatccgg acggcgagat gatcgccatc tatcgcaaga tccacatgtt
     1381 cgacgtcacg ctcgacggcg tcgcctccta caaggagtcg gagacggtcg cgccgggcga
     1441 ggagatcgtc accttcgagc tggacggcgt tacggtcggt ttggcgacgt gctacgacct
     1501 gcgcttcccc gagatcttcc ggatcctggc cctgcgcggc gccgaggtga tcctgttgcc
     1561 ggccgcgttc accatgacga ccggcaagga ccactgggag gtgctgatcc gggcgcgggc
     1621 gattgagaac caggtctacc tggttgcggc cggccagttc gggccgaatc tgccgggcaa
     1681 gtggtgctac gggcggtcga tgatcgtcga tccctggggc acggtactgg cgaccgcgcc
     1741 ggacacggag tcggtgatca cagccgtcgt cgatcggaat cacctccagt cgatccgccg
     1801 ccagatcccg tccctcgcca accgccaacc cgagacctac cgctggccgg acgaggtgtt
     1861 ggtcgcgcgg tagcggggtc ggagatgacc gaaccctgac aacggacgat gtatacccaa
     1921 ccggcgccgg gacgccggtt gggtattcga gtgcagcccc atcttcagtg gtcacccggc
     1981 tatggcacga ttttgtagat ggcgccgttg aggtcgatca aatagagctc gccctccgcg
     2041 tcttcggcga aggagctgat cgagagattg gtctcgaccg gggctgaggc cttgcccgtt
     2101 tccggattga cggcccagac gagccctgag cagtagtcgc cggcgaggta ggcgccggcc
     2161 aggctgggga tggccttgcc gcgatagacg tagccgccgg tgatcgagca gcccccttcc
     2221 tggtgcgtgt acgagaagaa gggcatggtc aggcctgtct ggtcgcagtt gtcctgctcg
     2281 tagcaatgcg gcccttccat g
//
LOCUS       DASSGX010000388         3848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_722458, whole genome shotgun sequence.
ACCESSION   DASSGX010000388 DASSGX010000000
VERSION     DASSGX010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722458"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2033
                     /locus_tag="VFI12_06895"
     CDS             <1..2033
                     /locus_tag="VFI12_06895"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET7056173.1"
                     /translation="ARSVEGTSVDALIGALANRHMLLVLDNFEHLIGAAVDIADLLNR
                     CRRLTVLVTSRTLLRVSGERAYPVPPLGLPDDSSSTLDQIAGTEAVRLFVTRATAIRP
                     DFALTDANATEAAAICRRLDGLPLAIELAAARTSALTPAELRTRLDRSLPLLTGGPRD
                     SPERQQTMRTAIAWSYELLTEPEQRLFRRLAVFAGGFGLEAAEAVAARDPDIDALDVI
                     TALVEKSLLRPVSGPEGQARFLMLETIREYGLEQLAAAGEEQAARAAHAGYYLALAER
                     TAQDRTGGEYGHWIAVFAHEIDNVRAAHRHFEQTGNTVAELMITAYLGWYWSEYRYRE
                     GLERLERALAKSPPASLDVRTLGFAWAGHFAAALGDIDAAERYAVEALPLARELGDSR
                     RMALTLMLLSNVAIERGDISSGREYLEQVLALPRKEGPDRGIASTLQGLGYMAMVTGD
                     FTRAQELISESLALNRSVGNTVELARGSGLLGLTLALAGDIPQAARLAQAQLDACLQN
                     GWDHAFDCPLAIAVHTGQFELAARLLGADELFAERTGHMRHGDELFRDTWERMVRTTR
                     DALGPELYDQARVTGRSTPRPQLRADVLAFVRSLADPPRGDVAAVDTFGLTPRELDVL
                     RLVAQGKSNQEIADTLFISVPTTKVHVRAILTKRNLDSRTAAAAFAIRNGLA"
     gene            2172..2408
                     /locus_tag="VFI12_06900"
     CDS             2172..2408
                     /locus_tag="VFI12_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056174.1"
                     /translation="MQTIPSTQGRIRTFASVALIACAIATSSLIGFGGTAAAQDPTMQ
                     VIQRHRGTPLTFTAQPARSGRSTYLHWRTGFVAP"
     gene            complement(2482..3408)
                     /locus_tag="VFI12_06905"
     CDS             complement(2482..3408)
                     /locus_tag="VFI12_06905"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET7056175.1"
                     /translation="MSARNLAALVLLAAIWGSSYLFIRVAVIPLGPLMVALARVGTAG
                     LGLAGYALLTRKRDDLARLDRRFLMLGLGNAAVPYVLIAFAELHLTASMAGILNATTP
                     LFAAVVVAAWSHERITSRVSLGIALGIVGVAVLVGWNPGPLTGIVLLAVLAMLLSSFS
                     YAVTTVYAKRSLRGVSSLRAAMGQQVGAVVLLAPFTAGAAVTGAGEGTPSLKVVLAVL
                     ALGLLCTSFAYLLYFHLIAAVGPVNTASTTFLIPVFSILWSAIFLDEAIRPVMLLGLA
                     IILGSVTLVTGQRMPGAGAPRTFLRREQASSE"
     gene            complement(3577..>3848)
                     /locus_tag="VFI12_06910"
     CDS             complement(3577..>3848)
                     /locus_tag="VFI12_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056176.1"
                     /translation="LQKLGMAPAKINLVVTWQEAGIFSEREEAALAWAESLTLLAETH
                     VPDAAYDAARAVFSEHELAALTVAVATINVWNRFNVAYRFPPEIG"
BASE COUNT          694 a         1355 c         1222 g          577 t
ORIGIN      
        1 tcgctcgctc ggtcgaggga acatcggtag acgccttgat cggagcactc gcgaaccggc
       61 atatgctgct ggtgctcgac aacttcgagc acctcatcgg cgcggcagtg gacatcgcgg
      121 atctgctgaa cagatgcagg cgactgactg tgctcgtaac gagtcggacg ctgctgcgcg
      181 tcagcggcga gcgggcgtat cccgttccgc cgctcggatt gcccgacgac tcaagctcca
      241 ctctcgatca gattgccggg acagaggcgg tccggctctt cgtgacccgc gcaactgcga
      301 tccggccgga tttcgcgctg accgatgcca acgccaccga agccgcggcg atctgccgac
      361 gtctcgatgg gctgccgctg gcaatcgaac tcgccgccgc ccgcaccagt gccctcaccc
      421 cggcggagtt acgcacgcgc ctcgatcgct cgcttcccct gttgaccggc ggcccacggg
      481 actcgcccga acgacagcaa acaatgcgca ccgccattgc ctggagctat gagttgctga
      541 cggagccgga gcaacgcctg ttccgccgcc tcgccgtatt cgccggcggg ttcggactcg
      601 aggcagccga agcggtcgcc gcccgagatc ccgacatcga cgcgctcgat gtgatcacgg
      661 cgctcgtcga gaagagcttg ctccgcccag tcagcggacc cgaaggtcag gcccgcttcc
      721 tgatgctgga gacaatccgt gagtacggcc tggaacaact cgccgcagcc ggcgaggagc
      781 aggctgcccg ggccgcgcat gctggctact acctggcgct tgccgagcgg accgcgcagg
      841 acaggaccgg cggcgagtat gggcattgga tagccgtctt cgcccatgaa atagacaatg
      901 tgcgagccgc gcaccgccat ttcgagcaaa ccgggaacac ggtggcggag ctcatgatta
      961 ccgcctacct gggctggtac tggtccgagt accggtatcg cgaggggctg gagcgtctcg
     1021 agcgagcctt ggccaagtcc ccgccggctt cgctcgacgt gcggacattg ggattcgcgt
     1081 gggccggcca cttcgcggcc gcgttgggcg atatcgatgc ggcagagcgg tacgcggtcg
     1141 aggcactgcc actggcccgg gagttgggcg acagcaggcg aatggcgctc acgcttatgc
     1201 tcttgtcaaa cgtggcgatt gagcgcggag acatcagttc gggtcgggaa tatctggagc
     1261 aggtgctcgc gctgccgcgc aaagagggac ccgaccgagg aattgcgagc accttgcagg
     1321 gtctcggcta catggcgatg gtcacaggcg atttcaccag ggcgcaagag ctgatctcgg
     1381 aaagtctcgc gctgaaccgc tcggtcggca acaccgttga gctggcgcgc ggatcggggc
     1441 tcctcggcct gacacttgcg ctggccgggg acattcccca ggcggcacga ctcgcgcagg
     1501 cgcaactcga cgcatgcctg cagaacggct gggaccacgc gttcgattgt ccactcgcga
     1561 tcgcggtcca caccggccag ttcgagctgg cggcacgttt gctcggcgcc gacgagctgt
     1621 ttgccgagcg cacagggcat atgcgccatg gggacgagct ctttcgcgat acctgggaac
     1681 gcatggtccg aacaacccgg gacgcgctgg ggccggagct gtatgaccag gcgcgggtta
     1741 cgggacgatc tacaccgcgg ccccagcttc gcgccgacgt ccttgccttc gtcaggtcgc
     1801 tggcggaccc gccgcggggt gatgtcgcag cagtcgatac cttcggcctc actccccgcg
     1861 agctggacgt cctgcggctc gtcgcgcaag gcaagtccaa ccaggagatc gccgacaccc
     1921 tcttcatcag cgtgccgacc acgaaggtcc atgtccgcgc gatcctgacc aagcgcaacc
     1981 tcgactcccg cactgccgcc gcggccttcg ctatccggaa cggcctcgcc tgagcctgcc
     2041 tacgtctttg gacgtattcc tgcccatccc ctgccgatac gccttcgagt cgatgttccc
     2101 cgtcctgccg aagcgcagga tggggccacg gtcgatcgcg gtgatcggcc ggacgcagag
     2161 ggagcacaga gatgcagacg ataccttcca cccagggccg catccgcacg ttcgccagcg
     2221 tggcgctgat cgcatgcgca atcgcgacca gttccctgat cggtttcggc gggaccgccg
     2281 ccgcgcagga cccgacgatg caggtcatcc agcgtcatcg cggcacgccc cttaccttca
     2341 ccgcgcaacc ggcccggtcc ggccggagca cctacctcca ctggcggacc ggcttcgtgg
     2401 ccccgtaacg ctcccatccc acaatccacg ggtccaggaa caggatgtcg tccaacccgc
     2461 gacgtcctgt ttccatgtcc gctattcgct cgatgcctgt tcgcgccgca agaacgtccg
     2521 gggcgcgccg gcacccggca tgcgctgccc ggtgacgagc gtcacgctgc cgaggatgat
     2581 cgccaggccg agcagcatca ccggccggat cgcctcgtcg aggaagatcg cgctccagag
     2641 gatgctaaag accgggatca gaaaggtcgt gctggcggta ttgaccggcc cgaccgccgc
     2701 gatcaggtgg aaatagagca ggtaggcgaa cgaggtgcag agcaggccga gcgccagcac
     2761 ggccagcacg accttgagcg acggcgtccc ctcaccggct ccggtcacgg ccgcccccgc
     2821 cgtgaacggc gcgagcagca ccaccgcgcc gacctgctgg cccatcgccg cccgcagcga
     2881 tgacaccccg cgcaacgacc gcttcgcgta gaccgtcgtc accgcgtaag agaacgacga
     2941 cagcagcatc gccagcacgg ccagcagcac gatcccggtc agcggccccg gattccaccc
     3001 gaccagcacc gccacgccga cgatccccag cgcgattcca agcgagaccc gcgaggtgat
     3061 gcgctcgtgg ctccaggccg ccacgaccac cgccgcgaaa aggggcgtgg tcgcgttcag
     3121 gatgcccgcc atcgacgccg tcaggtgcag ctccgcgaag gcgatcagga cgtacggcac
     3181 ggccgcattg cccaggccga gcatcaggaa gcgccgatcc agccgggcca ggtcatcccg
     3241 cttccgggtc agcagcgcgt acccagccaa gccgagcccg gccgtcccga cccgcgccag
     3301 ggccaccatc aacggcccga gcggtatgac cgccacccgg atgaacaggt aggaggaacc
     3361 ccagatcgcc gccagcagca ccagggccgc caaattccgc gcgctcaatg gacagatttc
     3421 tccgtaccga aggggcgatc gctcaaaaaa cggtgggcgg ggttatcatg ccccgcccgg
     3481 acccggccgc accgttggag ggaacccaac ccgcttccgg atcgactcca tcgaccgcgc
     3541 gtggggatcc ccgctacgtc aatatgcgaa agagatctac ccgatctccg gcgggaaccg
     3601 gtaggcgacg ttgaagcgat tccagacgtt gatcgtcgcg accgccaccg tcaaagcggc
     3661 cagctcatgc tcggaaaaga ccgcgcgagc agcatcatac gcggcgtccg gcacatgcgt
     3721 ctcggccagc agcgtcagcg actccgccca ggccagggca gcctcttcgc gctccgaaaa
     3781 gatacccgcc tcttgccagg tcacgaccag gttgatcttc gccggggcca tgcccagctt
     3841 ctgcaggt
//
LOCUS       DASSGX010000389         2073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1048898, whole genome shotgun sequence.
ACCESSION   DASSGX010000389 DASSGX010000000
VERSION     DASSGX010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048898"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="VFI12_06915"
     CDS             <1..491
                     /locus_tag="VFI12_06915"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF012912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HET7056177.1"
                     /translation="SGPFPTELHDETGQLIRERGREYGTTTGRPRRTGWFDAVAARHV
                     VRLNGVTEITLTLLDVLDALEEIQVCTSYDLGGEKLTLIPASTERLSHAKPTYAAQPG
                     WMQDITGARAASDLPERALAYVREIESLVGAPITMVGVGPAREQLVALSANAAMNAAA
                     AA"
     gene            507..947
                     /gene="fabZ"
                     /locus_tag="VFI12_06920"
     CDS             507..947
                     /gene="fabZ"
                     /locus_tag="VFI12_06920"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HET7056178.1"
                     /translation="MSYQAPLDAVEIMKIIPHRYPFLLVDRIDELEAGKRAVGVKNVS
                     MGEPFFQGHFPDYPVMPGVLIVEALAQVGAVAMLVMEEYQGKIAFFAGLDNVRFKRQV
                     KPGDTLRLEVELGQIRRNIGTGTAIATVNGEVACKGEFMFAIPN"
     gene            964..>2073
                     /locus_tag="VFI12_06925"
     CDS             964..>2073
                     /locus_tag="VFI12_06925"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HET7056179.1"
                     /translation="MLPIVGIDASRYPGEIRTGTETYSRELLDAMAELDSLPFEVRLY
                     VNRIDAGADEDLARLGNVRRLPFPRLWTHGRLSFEMLRDRPDLLFVPSHVIPLAHPRS
                     VVTIHDLGYLHEPEAHPVRQRRLLDLTTRWNARVSAHIIAISDTTRADLIQRYGAPAE
                     KITVIHHGVNDRFVPATPDEQTRVRYKWQLPDRFVLAVGTIQPRKNLARLAEAVARLQ
                     GDFPGLALVVAGKRGWMADSVLSDISRTLSSGLIHELGYVPLNDIPGLYSAASVTALV
                     STYEGFGLPALEALACGSPLVISDTPALVEVAGKAARIARAASVDEIAQGIEASLNEA
                     NNETGKRLGMVHAAAFTWANTAQQTRDVLNHVLENR"
BASE COUNT          379 a          714 c          629 g          351 t
ORIGIN      
        1 gtagcggccc cttcccgacg gagctgcacg acgagaccgg ccagctgatc cgcgagcgag
       61 gccgcgagta cgggacgaca accggccgcc cacgccgcac cggttggttc gatgctgtcg
      121 cggcgcggca cgtcgtccgg ctcaacggcg taaccgagat cacgttgacg ctcctggacg
      181 tgttggacgc actcgaagag atccaggtct gcaccagcta cgatctcggt ggcgagaagc
      241 tcaccttgat cccggcaagc accgagcgcc tatcgcacgc gaaacccacc tacgctgccc
      301 agccgggatg gatgcaggac atcaccggcg cgcgcgccgc atccgatctg ccggagcgcg
      361 cgctcgcgta cgtcagggaa atcgaatcac tcgtcggcgc tccaattacg atggttggtg
      421 tcggcccggc ccgggaacaa ctggtcgcgc tctcggcgaa tgccgcgatg aacgcggccg
      481 ccgctgcata gggagaagga gcgttcatgt cgtatcaggc gccgctcgat gccgttgaga
      541 tcatgaagat catcccgcac cggtatccat tcctgctggt cgaccggatc gacgagctcg
      601 aggccggcaa gcgcgcggtc ggcgtcaaga atgtctcgat gggcgaaccc ttcttccagg
      661 gccacttccc ggattacccg gtcatgcccg gcgtcctgat cgtcgaggcg ctcgcccagg
      721 tcggcgccgt cgcgatgctg gtcatggagg agtaccaggg caagatcgcc ttcttcgccg
      781 ggctcgacaa cgtccgcttc aagcggcagg tcaagccggg cgacaccctc cgcctcgagg
      841 tcgagctcgg ccagatccgc cgcaacatcg ggaccggcac ggcgatcgcc acggtcaacg
      901 gcgaagtcgc ctgcaagggc gagttcatgt tcgcgatccc gaactagagc gcccacaacc
      961 aacgtgctgc ccatcgtcgg catcgacgcc agccggtacc cgggcgaaat ccgcaccggc
     1021 accgagacgt attcccggga attgctcgac gcgatggccg aactcgatag tctgccattc
     1081 gaggtccgct tatatgtcaa tcggatcgac gcgggggccg acgaagatct cgcgcgcctc
     1141 ggaaacgtcc gacgcttgcc gttccctcgc ctttggacgc atgggaggct ctcgttcgag
     1201 atgcttcgag atcgaccgga cctgctcttc gtcccctctc acgtcatccc gctcgcccat
     1261 ccccgctccg tcgtcaccat ccacgatctc ggctatctgc atgaacctga agcgcacccg
     1321 gttcgacagc ggcgcttact ggacctcacc acgcgctgga acgcgcgtgt gtcggcccac
     1381 atcatcgcga tctcggacac taccagggcc gacctgattc agcggtacgg cgctccggcc
     1441 gagaagatca cggtcatcca ccacggcgtg aacgatcggt tcgtacctgc cacgccggac
     1501 gagcagacgc gagtccggta caaatggcaa ctaccggacc ggttcgtgct ggcagtcggc
     1561 acgatccagc cgcgcaagaa cctcgctcgg ctcgccgaag ctgtcgcgcg actgcaaggc
     1621 gatttcccgg gcctcgccct ggtagtcgct ggcaagcggg gatggatggc cgactcggtc
     1681 ctatcggata tttcccgcac cctttcgtcc ggactcattc acgagctggg atatgtcccg
     1741 ttgaacgata tccccggtct ttactccgcc gcttccgtca cggcgctggt ttcgacctac
     1801 gaaggcttcg gattgccggc gctcgaagcc ctcgcctgtg gatccccgct cgtcatctcc
     1861 gacacacccg cgctcgtcga ggtggctggc aaagccgccc ggatcgcgcg tgcagcatcg
     1921 gtcgatgaga tcgcccaggg tatcgaggcg tctctgaacg aggcaaacaa cgaaacgggc
     1981 aagcgcctgg gcatggtgca cgcggccgcc tttacctggg cgaacaccgc ccaacagacg
     2041 agagacgtac tcaatcacgt gctggaaaac cgc
//
LOCUS       DASSGX010000390         6270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1048913, whole genome shotgun sequence.
ACCESSION   DASSGX010000390 DASSGX010000000
VERSION     DASSGX010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6270
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1048913"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="VFI12_06930"
     CDS             complement(<1..900)
                     /locus_tag="VFI12_06930"
                     /inference="COORDINATES: protein motif:HMM:NF019156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056180.1"
                     /translation="MRALLTKVFGDANQKLLNQLQPIVDEINDLEPEMEQASDGELRA
                     MGDEFRSRLAGGETLDDLLPEVFAAVREIAKRVSGKRHYDVQLIGGIVLHQGKIAEMK
                     TGDGKTLTATLPIALNALEGKGVHLVTVNDYLAKRDTQWMGPIYHELGLSVASSQHDE
                     AFMFDPEWESEDERLAFLRPITRQEAYAADITYGTNNEFGFDYLRDNMVVSADQCVQR
                     DLNYAIVDEVDNILIDEARTPLIISGQAERATDRYYQFAQIVKQLRPNRHYEVDVKHK
                     SVTLTDEGVDKVEQLAEIPAGESI"
     gene            1009..1425
                     /locus_tag="VFI12_06935"
     CDS             1009..1425
                     /locus_tag="VFI12_06935"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HET7056181.1"
                     /translation="MPKVANMRENGSKFIDRVHEVVARIPFGRVTTYGAIARSLGEAR
                     AARMVGWAVNSPPPELNLPCHRVVNRIGFLSGGWHFGHPDVMRDALLAEGITFVAEYQ
                     VDLSRYFWDPSLESAATDEMDDLKDISGFEKRSGEF"
     gene            complement(1575..2153)
                     /locus_tag="VFI12_06940"
     CDS             complement(1575..2153)
                     /locus_tag="VFI12_06940"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET7056182.1"
                     /translation="MILDRAGARSDVAEVAAAAIRGGADVVQVREKESPTPEVVEIVR
                     RVILAVGDPLKIAVNGFPGIAAEFGTHVHLPEVHGPIGEEVRLADGALLSRSIHGPTR
                     RPRADYAILGNLLETGSKPGKAGLGFARFGAIARACPVPVLAIGGVEPRHVADVLRHG
                     GHGVAVRSFVIGSQDPEAAARQIRMEFDKWAK"
     gene            complement(2180..2812)
                     /gene="thiE"
                     /locus_tag="VFI12_06945"
     CDS             complement(2180..2812)
                     /gene="thiE"
                     /locus_tag="VFI12_06945"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET7056183.1"
                     /translation="MRPDLASRLRLYLVADPDYVHGDLLSTVGHALNGGVTAVQLRYK
                     HGTDRAAMELARTLRSITVDHGALFLVNDRLDLALASGADGIHLGVDDLPLEDARRIG
                     GPDLIIGYSPETDEQTQDAAERGANYLGVGPIFGTATKSDAGLAIGLETLNRRSELAG
                     VPVVGIGGIRAQNARSVIDAGAIGVAVVSAISMQENPKAAAVALRRALDQ"
     gene            complement(2802..3587)
                     /locus_tag="VFI12_06950"
     CDS             complement(2802..3587)
                     /locus_tag="VFI12_06950"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HET7056184.1"
                     /translation="MVRNDTPLVIAGTELSSRLFVGTGKYRNSGEMNAAFDASGTNLI
                     TVALRRIDFDDPKSRSVLEDVDWKRYRILPNTAGCQTAEEAIRIARMARAMELSDWIK
                     LEVIPDPKYLLPDPIGTLEAAQVLIDEGFVVLPYIGADPILAKRLQEMGTATVMPLGS
                     PIGSGQGILNLEAIKIIIEQATVPVVVDAGIGTPSDAAIAMEAGADAVLINTAIALAE
                     DPAAMADAMRQGVEAGRKAYLAGRIPVKPYASASSPTTNLVAS"
     gene            complement(3577..4458)
                     /gene="thiD"
                     /locus_tag="VFI12_06955"
     CDS             complement(3577..4458)
                     /gene="thiD"
                     /locus_tag="VFI12_06955"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HET7056185.1"
                     /translation="MTTDLTYANPMGHPGVPRAMTIAGSDSGAGAGIQADLKTFAAHG
                     VYGTSVLTAVTAQNTTSVLAIAEVPEEVIVHQIDGVLEDIGTDAIKTGMLSSASIIEV
                     VADRIEAWGVPNVVVDPVMIAKSGDALLQPTAVRTLVKRLLPLALIVTPNIPEAEVLS
                     GKHIINAASEEEAARIINRLGPKYVVIKGGHRESDDAVDLVYDGASFERIGGPRISTR
                     NTHGTGCTFSAAIAANLALGRNPLEAITRAKEYLSGALRESFSIGEGHSPVNHFHAVP
                     QIDPRGTTVASGGSHGS"
     regulatory      complement(4463..4570)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4677..5021
                     /locus_tag="VFI12_06960"
     CDS             4677..5021
                     /locus_tag="VFI12_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056186.1"
                     /translation="MNVIEGLRIVRQRLGENDAEDATLAHVDQILKRASVPSVGNASA
                     QSLLQLTRMLMRHPTAAANSAVYNDLALLEEQLESAAVAGAKRAAEEADKPMPKTKKY
                     YKELKEKQKKAE"
     gene            5059..>6270
                     /locus_tag="VFI12_06965"
     CDS             5059..>6270
                     /locus_tag="VFI12_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7056187.1"
                     /translation="MVEAALDPNQLIPRVIGRYSPVTVDHGEGIYVWDTDGNRWTDFT
                     SGIAVTNTGHCHPRVVAAIQEQAAKIIHAQQNIFAHEPMLKAAAALTETLPERLNQVF
                     WANSGAEAIEGAIKLAKVATGRSAIIAFRGAFHGRTHAAMSVTSSRAKVRGHYEPLLA
                     GIYFAPYPYLFRNPYKGSVEEADLAYFNELETLFETMVMPDDVAGIIVEAIAGEGGYM
                     VPTARWFQKVREICDKYGIMLIVDEIQTGVGRTGKMWGFEHFGIEPDIITIAKGIASG
                     MPVSAVVSDKAIMDKWAPGAHGGTYGGNAVGTAAAYATLQVMKDENLVGNAATMGAVL
                     KAGLEEIQARNPVIGDVRGKGLMLATEFVTPIGAPNPEAVARVIQKCQDEKVLLLNCG
                     TYDQAIRIIPPL"
BASE COUNT         1123 a         1971 c         2028 g         1148 t
ORIGIN      
        1 gatgctctcg ccggctggaa tctctgcgag ctgctcgacc ttgtccacgc cctcgtcagt
       61 gagcgtgacg ctcttgtgct tgacgtcgac ttcgtaatgc cggttcggcc gcagctgctt
      121 gacgatctgg gcgaactggt aatagcggtc ggtggcgcgc tcggcctggc cagagatgat
      181 cagcggcgtc cgggcctcat cgatcaggat gttgtccacc tcgtcgacga tcgcatagtt
      241 cagatcgcgc tgcacgcact gatcggcgct gacgaccatg ttgtcgcgaa ggtagtcgaa
      301 cccgaactcg ttattggtcc cgtaggtgat atcggcggcg taggcctcct ggcgcgtgat
      361 cgggcgcagg aacgcgagac gctcgtcctc ggattcccat tcggggtcga acatgaacgc
      421 ttcgtcatgc tggctcgatg cgaccgacag gccgagctcg tggtagatcg ggcccatcca
      481 ttgcgtgtcg cgcttggcca ggtagtcgtt gacggtgacg aggtggacgc ccttgccctc
      541 aagcgcgttg agcgcgatcg gcaatgttgc agtcagcgtc ttgccgtcgc cggtcttcat
      601 ctcggcgatc ttgccctggt gaaggacgat gccgccgatc aattgcacgt cataatggcg
      661 cttgcctgag acgcgcttgg cgatctcgcg gaccgccgcg aagacttccg gcaggagatc
      721 atcgagcgtc tcgccacctg ccaggcggga gcggaactcg tcacccatgg cccggagctc
      781 gccgtcgctg gcttgctcca tttcgggctc aaggtcattg atctcgtcaa cgatcggctg
      841 gagctggttg agtaactttt ggttggcgtc gccgaagact ttggtcaaga gggcgcgcat
      901 ggagtgtaga cctttcgatg tgaaagacgg agagagtcgc gcagttatga ggcgctccga
      961 tcatagaagt atagcgggtg cgggaaggct gacccggacg acgcggccat gccgaaggta
     1021 gcaaacatgc gggaaaacgg ctccaaattc atcgaccgtg tgcacgaagt ggtcgcacgg
     1081 atcccgttcg ggcgagtcac cacgtatggc gcaattgccc ggtcgctggg cgaggcacga
     1141 gcggcgcgga tggtcggctg ggcggtcaat tcgcccccgc cagagttgaa cttgccgtgc
     1201 caccgggtcg tgaaccggat cggcttcctg agcggcggct ggcacttcgg ccaccccgac
     1261 gtgatgcgag acgccctgct cgccgaggga atcaccttcg ttgccgaata ccaggtcgat
     1321 ttgagccgct atttctggga cccgtcgctc gaatcagccg ccaccgacga aatggacgat
     1381 ctcaaggaca tctccggatt cgagaagcgt tccggcgaat tctgagcggc tgaggattga
     1441 tccattgcgc tcgaccacaa ccagcttctc gcgcaggtca agttcatcca gcagcccgga
     1501 aaccggcaag ccggcgctga ccgcgcgttc tttgccgttg agccggatat tgatcattga
     1561 tgtatccggc gcagtcactt cgcccacttg tcgaactcca ttctgatttg ccgcgccgcg
     1621 gcctccggat cctggctccc gatcacgaag gagcgcaccg cgacgccgtg gcccccatgc
     1681 cgtagcacgt ccgccacgtg ccgtggttcg acaccgccta tggccagaac cggaaccggg
     1741 catgctcggg cgattgctcc gaacctcgcg aatccgaggc cggccttgcc cggcttgctc
     1801 ccggtttcca gcaagttgcc gaggattgcg tagtcggcgc gcggtctgcg ggtcgggccg
     1861 tggatcgagc ggctcaacag cgcaccgtct gccaaccgga cttcttcgcc gatcgggccg
     1921 tgcacttccg gcaggtgcac gtgcgtcccg aactcggcag cgatcccggg gaacccgttg
     1981 actgcgatct tcagcgggtc gccgaccgct aatatgaccc ggcggacgat ttccacaact
     2041 tccggggtag gcgactcctt ttcgcggacc tgcacgacgt cggcgccgcc ccggatcgcg
     2101 gccgccgcga cttcagcgac gtcgctccgg gctccggcgc gatcaaggat cacgacgaac
     2161 ctcggggtct ttagggcggt cattgatcca gcgcacgacg cagggcgact gcggccgcct
     2221 tcggattctc ctgcatcgag attgcggaga cgacagcgac gccgattgcg ccggcatcga
     2281 tcacggaccg ggcgttctgc gcgcgtattc cgccaattcc gacgaccggg acgccggcga
     2341 gctcggagcg ccgattgagc gtctcgagtc cgatggccaa accggcatcc gattttgtcg
     2401 cggtgccaaa gatcggaccg acgccaaggt agttcgcccc gcgctcggct gcgtcttgcg
     2461 tctgctcgtc tgtctcaggt gaataaccga tgatcagatc gggcccgcca attcgccgcg
     2521 cgtcttccag cgggaggtca tcgacgccga ggtggatgcc gtccgctccg ctcgccagcg
     2581 cgaggtctag ccggtcattc accaggaaca gcgcgccgtg atccaccgtg atcgaacgca
     2641 aggtccgggc cagctccatt gccgcgcggt ccgtcccgtg cttgtaccgg agctgcaccg
     2701 ccgtcacgcc gccattcagc gcgtgcccga ccgtcgagag caggtcgccg tggacgtaat
     2761 ccgggtcggc gaccaggtag aggcgaaggc gcgacgccag atcaggacgc aacgagattc
     2821 gtggtcgggc tgctggccga agcgtatggc ttgaccggga tccggccagc caggtacgcc
     2881 ttgcgacctg cctcgacgcc ctgcctcatc gcatcggcca tcgctgccgg gtcttccgca
     2941 agggcgatcg ccgtgttgat caggaccgcg tcggccccgg cctccatcgc gatcgcggcg
     3001 tcgctcggcg tgccgatgcc ggcatcgacc acgaccggaa ccgtcgcctg ctcgatgatg
     3061 atcttgatcg cctccaggtt caggatgccc tgtcccgatc cgatcgggga gccaagtggc
     3121 atgaccgtgg ccgtgcccat ctcctgcagt cgtttcgcca aaatcgggtc ggcgccgatg
     3181 tagggcagga cgacaaagcc ctcgtcgatc aggacctgcg ctgcctcgag cgtcccgatc
     3241 gggtccggga gcaggtactt tggatcggga atcacttcga gcttgatcca atccgacagt
     3301 tccatggccc gggccatccg cgcgatccgg atcgcctctt cggcggtctg gcaaccggcc
     3361 gtgttcggca ggattcggta gcgcttccag tcgacgtcct ccagcacaga gcggctcttg
     3421 ggatcgtcga agtcgatccg gcgcagcgcg acggtgatca ggttggtgcc cgacgcgtcg
     3481 aacgcggcgt tcatctcacc cgagtttcgg tacttgccgg tgccgacgaa gagccggctg
     3541 gaaagctcgg ttccggcgat cacgagtgga gtgtcgttac gaaccatggg atcctcctga
     3601 tgcgacggtc gtgccgcgcg ggtcaatttg ggggacggcg tggaagtgat tgaccggcga
     3661 atgcccctcg ccaatcgaaa acgactcgcg gagcgctccc gagaggtatt ccttcgcacg
     3721 ggtgatcgcc tccagcggat tgcggccgag ggcgagattc gcggcgatcg cggccgaaaa
     3781 cgtgcagccg gttccatgcg tgttgcgagt cgagatcctc ggccctccga ttcgctcgaa
     3841 tgatgcaccg tcatagacga ggtcgaccgc gtcgtccgat tcgcggtgac cacccttgat
     3901 cacgacgtac ttcggaccga gccggttgat gattcgggca gcttcttcct ccgatgcggc
     3961 attgatgatg tgcttcccgc tcaacacctc ggcctcgggg atgttcggcg tgacgatcag
     4021 cgccagcggc agcaaacgct tgaccagcgt gcggaccgcc gtcggttgga gcagggcatc
     4081 gccactcttg gcgatcatca ccgggtcgac gacgacattc gggacgcccc acgcctcgat
     4141 ccggtcggcg acgacttcta tgatcgacgc gctggaaagc atgccggtct tgatggcgtc
     4201 ggtcccgatg tcctcaagga cgccgtcgat ttgatggacg atgacctctt cgggcacctc
     4261 cgcgatggcg agaaccgagg tcgtgttctg ggccgtcacg gcggtcagaa ccgatgtgcc
     4321 gtagacgccg tgggcggcga atgtcttgag gtcggcctga atgcccgcgc cggcaccgga
     4381 atcggaaccg gcgatagtca tcgcgcgcgg tacgccggga tggcccatcg gattcgcgta
     4441 tgtcaaatct gttgtcatgt ctggtaattc atcagcgcct tcctacgccg gtacgagccg
     4501 gatcaggttc cgagggttgg cggcccgatg ccgcgctctc agcgtccaaa acgcacccct
     4561 ggcacatcgc aattttacca ttgaaacgac gacacgggac gtgcgtcggc cggaacagca
     4621 gagccggtgc gctagaattc cagcgcgtgc ccggccgctc tcaaaggatg cccaacatga
     4681 atgtgattga aggtctgcgg attgtccgcc agcgactcgg agagaacgac gccgaggacg
     4741 ctacgctcgc gcatgtcgac caaattctca agcgtgcttc ggtgccgtcg gtcggcaacg
     4801 cctccgcgca atcgctcctg caactgaccc ggatgctgat gcggcaccca acggcggccg
     4861 ctaactccgc tgtgtacaac gatctggcgt tgttggagga gcagctcgag tcggctgcgg
     4921 tagccggggc gaagcgggcg gccgaagagg ccgataagcc gatgccgaag acgaagaagt
     4981 actacaagga gctcaaggaa aagcaaaaga aggcggaata ggagagcatg tcccggatgc
     5041 gtaccaggga ggatatcgat ggttgaagca gcgctcgatc cgaaccaatt gattccgcgc
     5101 gtgatcggcc ggtactcgcc ggtcacggtc gaccacggcg agggaatcta cgtctgggat
     5161 actgacggta accggtggac tgacttcacg tccggcatcg ccgtcaccaa cacgggccac
     5221 tgccatcccc gtgtcgtcgc ggccatccag gagcaggcgg ccaaaatcat ccatgcccag
     5281 cagaacattt tcgcccatga gccgatgctc aaggcagcgg cggcgctgac cgagacgctg
     5341 cctgagcggc tcaaccaggt cttctgggcg aacagtgggg ccgaagcgat cgagggcgcg
     5401 atcaagttgg ccaaggtcgc tactggccgg tcggcgatca tcgccttccg aggcgcattc
     5461 cacggtcgca cccacgccgc gatgtcagtg acgtcgtcgc gggcgaaggt gcgcggccac
     5521 tatgaaccgc tgttggcagg aatctacttc gcgccctacc cgtacctctt ccgcaacccg
     5581 tacaagggca gcgtcgaaga ggccgatctc gcgtacttca acgaactgga gacgcttttc
     5641 gagacgatgg tcatgccgga cgatgtcgcg ggaatcattg tcgaggccat tgccggcgag
     5701 ggcggctaca tggtcccaac cgcgcgctgg ttccagaagg tgcgcgagat ctgcgacaag
     5761 tacggcatca tgctgattgt cgatgagatt cagaccggcg tcggccgcac cggcaagatg
     5821 tggggcttcg agcacttcgg catcgaaccg gacatcatca caatcgccaa gggcatcgcc
     5881 tccggcatgc cggtcagcgc ggtcgtttcc gacaaggcga tcatggacaa gtgggcgccg
     5941 ggcgcgcatg ggggcaccta cggcggcaac gcggtcggca cggctgctgc ctacgccacg
     6001 ctccaggtca tgaaggacga gaacctggtc gggaacgcgg cgacgatggg cgcggtgcta
     6061 aaggccggtc tcgaagagat ccaggcccgg aacccggtca tcggcgatgt ccgtggtaag
     6121 ggcttgatgc ttgcgacaga gttcgtcacc ccgatcggcg cgccgaatcc ggaggccgtc
     6181 gccagggtga tccagaagtg ccaggacgag aaggtgcttc tcctgaactg cggaacctac
     6241 gaccaggcga tccggatcat cccgccgctg
//
LOCUS       DASSGX010000391         2035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_504109, whole genome shotgun sequence.
ACCESSION   DASSGX010000391 DASSGX010000000
VERSION     DASSGX010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504109"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VFI12_06970"
     CDS             <1..258
                     /locus_tag="VFI12_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056188.1"
                     /translation="ARNSPGPDDAGAPTMRAERLRHQADGNPTRVEARHDTSLAESFA
                     PIASGDVSGSTASTAKGLNQLQSRRRKRRARRARQPYHRFD"
     gene            complement(353..1021)
                     /locus_tag="VFI12_06975"
     CDS             complement(353..1021)
                     /locus_tag="VFI12_06975"
                     /inference="COORDINATES: protein motif:HMM:NF012565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HET7056189.1"
                     /translation="MVLGLLALMQPIGAARADIALSELIVELRSGAHNREDVEIWNKG
                     PERAYVAVQPREIVDPGQAGQSAHEEPDPQKLGLLVAPARMILEPGQRKLVRIATLAS
                     DNGRERVYRVTVKPVAGPLASAASGLKVLVGYDVLVLVRPAVARPKVTGVRSGNRLTV
                     RNEGNVSVELTQGRQCELAKAVCRDLPGKRLYAGAEWTVTLPGSGPADYVVKSPVGQS
                     PIRF"
     gene            complement(1048..>2035)
                     /locus_tag="VFI12_06980"
     CDS             complement(1048..>2035)
                     /locus_tag="VFI12_06980"
                     /inference="COORDINATES: protein motif:HMM:NF027306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CS1-pili formation C-terminal domain-containing
                     protein"
                     /protein_id="HET7056190.1"
                     /translation="SLRNDEGIGKDYTIGPSVSWPILMRSGLQLVLQADAQKTRTTTA
                     AFVGFRAQLTRNDVSLVGTLGHAQRNTDDEDSLGDAGRAVGSLGATFFHEAPDRTQVS
                     ASAGFERNLDSSQIQAAASLHSRFGSARGDLLHRLGGGQGLQYGLTLQTGAAIDRHEV
                     ALGGRDIAESALVVSLDGESGTSRFDVLVNEQPRGRVAAGAPLPLFLEPYREYAVRLR
                     PVGAVSVDYDSGMRSVTLYPGNVQHLAWDTHAFFTVFGQALRPDGRPVANARIRSKRG
                     IGESDENGYFQIDVAVGDMLSFESAGSGSCSAPVSATRSEEEFVPLGKVTCQ"
BASE COUNT          364 a          717 c          654 g          300 t
ORIGIN      
        1 gctaggaatt cgcccgggcc cgacgacgcg ggggcgccga cgatgcgtgc cgagcggctg
       61 aggcatcaag ccgacggcaa cccaaccagg gtcgaggcaa ggcatgacac gagcctggcc
      121 gagtccttcg cgccgattgc gagcggcgac gtgagcggct cgaccgcctc caccgcgaag
      181 ggcctcaacc agctgcagag tcggcgacgt aagcgccgcg cacgacgcgc gcggcaacct
      241 taccaccgat tcgattagcc gccggacctt cgcttatggc agcgccaacc ggctgaccgc
      301 ggccttgggc ggggtcagca acctaaccgc actcgggcgg atgagcactg cctcaaaacc
      361 ggatcggact ctgcccgaca ggcgacttca ccacataatc ggccgggccg ctgccgggca
      421 gtgtcaccgt ccactccgcg cccgcataga gccgctttcc ggggaggtcg cggcaaacgg
      481 ccttggccaa ctcacattga cgcccctggg tcagctcgac gctgacattg ccctcattgc
      541 gaacggtcag cctgttcccc gaccggacgc cggttacctt gggccgtgcc accgccgggc
      601 gcaccaaaac gaggacgtca tagccgacca gaactttcag cccggacgcg gccgaggcga
      661 gcggaccggc aaccggcttg acggtgaccc gataaacgcg ttcgcggcca ttgtccgacg
      721 ccagcgtcgc gatccgtacg agcttgcgct ggccaggctc gaggatcatt cgcgccggag
      781 cgacgagcaa tccgagcttc tgcggatcgg gctcctcgtg cgcggactgg cctgcctggc
      841 cgggatcgac gatctcgcgc ggctggaccg cgacataagc gcgctcgggg cccttgttcc
      901 agatctccac atcctcgcgg ttgtgcgccc ccgagcggag ctcgacgatg agctcgctga
      961 gcgcgatgtc cgcccgcgcg gcaccgatcg gctgcattag cgcgagcagg ccgaggacca
     1021 tatgacgcgc ccgggcgccg aacccggtca ctggcaggtc acctttccaa gcggcacgaa
     1081 ttcctcctcc gacctggttg cactgaccgg ggcgctgcac gaacccgatc ccgcggattc
     1141 gaaggaaagc atatcgccga ccgcgacgtc gatctggaaa tagccattct catcgctttc
     1201 gccgatcccc cgcttcgatc ggatcctggc attggcgacg ggccggccat ccggccgcag
     1261 cgcctgaccg aacaccgtga aaaaggcatg ggtgtcccaa gcgagatgct ggacgttgcc
     1321 cggatagagc gtcaccgagc gcatcccgct gtcataatcg accgagaccg cgcccaccgg
     1381 gcggagcctg accgcatatt cgcgataggg ctcgaggaac agcggaagcg gcgctccggc
     1441 cgcgacccgg ccacgcggct gctcgttgac caggacgtcg aaccgggagg tgccgctttc
     1501 cccgtcaagc gacacgacca gcgcgctttc ggcaatgtcg cggccgccga gcgcgacctc
     1561 gtgccggtcg atcgccgcac ccgtctggag ggtaagccca tattgcaggc cctgcccgcc
     1621 gcccaggcgg tggaggaggt cgccgcgggc gctcccgaaa cgcgaatgaa gagacgcggc
     1681 cgcctggatc tggctcgaat cgaggttgcg ctcgaagccc gccgaggcgg aaacctgcgt
     1741 ccggtcaggc gcctcatgaa aaaaagtcgc gcccaaggag ccgacggcgc ggccggcatc
     1801 gcccagggaa tcctcatcgt cggtgttccg ctgtgcatga ccaagggtcc cgaccagcga
     1861 aacgtcgttc cttgtcagtt gcgcacggaa cccgacgaac gccgcggtcg tggtccgcgt
     1921 cttctgcgca tcggcctgga gcaccagctg gaggccgctg cgcatcagga tcggccagct
     1981 gaccgagggt ccgatcgtat aatccttccc aatcccctca tcgttcctga gcgag
//
LOCUS       DASSGX010000392         4165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_69976, whole genome shotgun sequence.
ACCESSION   DASSGX010000392 DASSGX010000000
VERSION     DASSGX010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4165
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69976"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1447)
                     /locus_tag="VFI12_06985"
     CDS             complement(<1..1447)
                     /locus_tag="VFI12_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HET7056191.1"
                     /translation="MTPDSSLDGLMQWRCIGPFRGGRVVAVTGSYSDRNTFYFGGCAG
                     GVWKTVDSGTYWRNVSDGFFSTSSVGAIAVAPSDSNVIYAGTGETTIRIDVSHGDGVY
                     KSTDEGRTWKNIGLRDTRHIGKIRVHPQDPDTVWVAALGHAFGPNKERGIFKSTDGGE
                     SWRQVLFVSEQAGAVDLTVDPANPRVLYAGVWEAHRTFWQISSGGPDSGLWQSLDGGE
                     TWENISGRLGLPKPIWGKVGVAASGATPGRAWALIEHKTEGGLYRTDDLGQTWEKVSE
                     DQNLLSRAWYYTHLTADPQDGNTVYVNNLDFHKSSDGGRTFEKIPTPHGDNHDLWIDP
                     LDNTRMIQGNDGGANVTLNGGYTFSSIYNQPTAQFYHLATDNRDPYTVYGTQQDNSSI
                     AVPSGVNHGAITWGDCFVAGSGESGYIAVHPENPDVVYVGAIGSSPGGGNSLQRYDRK
                     RDQIRLITTWPEGDRGHGAKDLKYRFAWTYPI"
     gene            complement(2046..3380)
                     /locus_tag="VFI12_06990"
     CDS             complement(2046..3380)
                     /locus_tag="VFI12_06990"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7056192.1"
                     /translation="MPATTDRRARRPQNSWAERFGLPHLTRPGAVTLYVATWIDAVGS
                     GLFLTFYLLYLTKAAGFSLGVAGTVLSISSGLALAANPIAGSLIDKIGARRMMLASQI
                     ICAAGYVGLLFVKGSIPLLVVASTMAVIGERIFWVGFPSFISIMAPDSERDRWFAFMG
                     MTREAGFGVGGLLAALIVALAGTDGYRILVLLNACSFAIAATIIWLRVPSPPLEPVHH
                     DHGGWKAVLRDRPVMKMAVANTVAVMTVLIGGLAMPIYVVDELDLPAWLPGLLFVATT
                     IVLAGGQSIGLRIVTGWLRTRVYLLAAAIWVIGAIAFAFAQVVPREILIPYMFFAIVV
                     TIGGDLFHAPQTNGLPSALAPAALRGRYLALFSLSWGVGRTVAPGIVSGLLWLGPTWP
                     WVGMVLMAGLAAVIALHTERELDPERQRMARSIHLEEPVADDLIEPVEPVAA"
     gene            3579..3845
                     /locus_tag="VFI12_06995"
     CDS             3579..3845
                     /locus_tag="VFI12_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056193.1"
                     /translation="MTKKQVALEVTMMLAAQQLVDRWLRSPGRGPFQRAVSRELRERW
                     NDLPGTEAQQRAFRRLANAIRVGEGHAIANPGPLLKIARQALHP"
BASE COUNT          732 a         1315 c         1412 g          706 t
ORIGIN      
        1 cgatcgggta ggtccaggcg aagcggtact tgaggtcctt ggcgccgtgt ccgcgatcgc
       61 cttcgggcca ggtcgtgatc aggcgaatct gatcccgctt gcgatcgtag cgttgcagtg
      121 aattgccgcc gccgggcgat gacccgatag cgccgacgta gacgacgtcg ggattctcgg
      181 gatgcacggc gatatagccg ctctcgccgc tcccggccac gaagcaatcg ccccaggtga
      241 tcgcgccgtg gttcacgccg ctcggcacgg cgatgctcga gttgtcctgc tgggtcccgt
      301 agacggtgta cggatcgcga ttgtcggtcg cgaggtgata gaactgggcg gtcggctggt
      361 tgtagatgct gctgaaggta tagccgccgt tcagggtgac gttcgcgccg ccatcgttgc
      421 cctggatcat gcgcgtgttg tcgagcgggt cgatccagag gtcatggttg tcgccatgcg
      481 gcgtcgggat cttctcgaac gtgcggccgc cgtcgctgct cttgtggaaa tcgaggttgt
      541 tgacgtagac cgtgttgccg tcctggggat cggcggtgag atgggtgtaa taccaggcgc
      601 gcgagagcag gttctgatcc tccgagacct tctcccacgt ctggccgagg tcgtccgtcc
      661 ggtacagccc gccctcggtc ttgtgctcga tcagggccca ggcgcggccg ggggtggcgc
      721 cggacgcggc cacgccgacc ttgccccaga tcggcttcgg caatccgagc cggccggaga
      781 tgttctccca ggtctcgccg ccatccaggc tctgccaaag tccgctatcc ggcccgccgc
      841 tgctgatctg ccagaaggtg cggtgcgcct cccagacgcc ggcgtagagc acgcgcggat
      901 tggcgggatc gaccgtgaga tcgaccgcgc ccgcctgctc gctgacgaac agcacctgcc
      961 gccaggattc gccgccgtcg gttgatttga agatgccgcg ttccttgttc gggccgaagg
     1021 cgtggccgag cgccgccacc cacaccgtgt cgggatcctg cgggtggacg cggatcttgc
     1081 cgatgtggcg ggtgtcgcgc aggccgatat ttttccaggt ccggccctcg tcagtgctct
     1141 tgtagacgcc gtcgccgtgg gagacatcga tgcggatcgt cgtttcaccg gtgccggcgt
     1201 aaatcacgtt cgaatccgag ggcgcgaccg cgatcgctcc gacggacgac gtagagaaga
     1261 atccatcgga gacgttgcgc cagtaggtgc cggagtcgac ggtcttccag acgccgccgg
     1321 cgcatccgcc gaagtagaac gtgttgcggt cggagtagct tccggtgacc gccacgacgc
     1381 gcccgccgcg gaatgggccg atgcagcgcc attgcatcag gccgtcaagc gaagaatccg
     1441 gcgtcatgtc tcaatccttg ttcacacgtg cgaaatcgtt gcggacacgg tagcgcaaat
     1501 tgcggcgacg tgaaggtgag gaggcgagca gttggggcgc gaagaccgtt ataggggttt
     1561 gcgggggcaa acccgccgct tcgcaacggg cctgttcgca tgcatatgta cccaaccgta
     1621 gcgccggccc ccgtggccaa cgctgtcgca ttaaccgctg cgcagcgctg tgagcagggt
     1681 cttgccggtg gcgtggagcc aggcgatcgc actgaccgtg gtggggtgcg gcggggactc
     1741 gccgctacag tgctgtgtgc aaacgtcaac caatggtttg cgcggaaaac aggatgtagc
     1801 gcgggttctg gaaaaacggc gcgatcccgt ccgcattccg ggtttcagtg ggtgaatcac
     1861 ccaaaagtgc cgaaaaaggg gtccattcgg acccgtgacg actagtcata cgcggctatc
     1921 gtgtgagtaa gccattccgg cggttgtgcc agccacacga tcgtcgcccc tttccctcga
     1981 gtggttttgc tgagacgccc ggcgccatcc cgccgggcgt ctttgcatct ccggaacgat
     2041 gtttcctacg cggcgaccgg ctcgaccggc tcgatcaggt catcggcgac cggctcctcg
     2101 aggtggatgc tgcgggccat gcgctgccgt tccgggtcca gctcgcgctc ggtgtgcagc
     2161 gcgatcaccg cggccaatcc ggccatcagc accatcccga cccacggcca ggtcggcccc
     2221 agccagagca ggcccgagac gatgccgggc gcaaccgtcc ggccgacacc ccacgacagc
     2281 gagaagagtg ccaggtagcg gccccggagc gcggccgggg caagggccga gggcagccca
     2341 ttcgtctgcg gcgcgtggaa gaggtcgccg ccgatcgtga cgacgatcgc gaaaaacatg
     2401 tatgggatca ggatctcgcg cggcacgacc tgggcaaacg cgaaggcgat cgcgccaatc
     2461 acccagatgg ccgcagcgag cagatagacg cgggttcgca gccagccggt cacgatccgc
     2521 aacccgatcg attggccgcc ggccaggacg atcgtggtcg ccacgaagag caggccgggc
     2581 agccaggcgg gcagatccag ctcgtcgacg acgtagatcg gcattgccag gccgccgatc
     2641 aagacggtca tcaccgctac agtattggcg acggccatct tcataaccgg gcgatcccgc
     2701 aggaccgcct tccagccgcc gtggtcgtga tggaccggtt ccagcggcgg cgaggggacc
     2761 cgcagccaga tgatcgtcgc ggcgatggcg aacgagcagg cgttgagcag gaccaggatc
     2821 cgatagccgt cggtgccggc cagtgcgacg atcagcgccg ccaataatcc gccgacgccg
     2881 aagccggcct cccgcgtcat ccccatgaag gcgaaccagc ggtcccgctc gctgtccggg
     2941 gccatgatcg agatgaagga ggggaagcca acccagaaga tgcgctcccc gatcactgcc
     3001 atcgtcgagg cgacgaccag gagtggtatc gagcccttga cgaaaagcag gccaacgtag
     3061 ccggcagcgc agatgatctg gctagcgagc atcatccggc gcgcgccgat cttgtcgatc
     3121 aggctgccgg cgatcgggtt cgcggcgaga gcgagcccgg acgagatcga gaggactgtc
     3181 ccggcgacgc cgaggctgaa tccggcagcc ttggtcagat agagcaaata gaaggtgagg
     3241 aagaggccgc tgccgacggc gtcgatccag gtcgcgacgt agagcgtgac cgcgccgggc
     3301 cgggtcaaat ggggcagacc gaagcgttcc gcccacgaat tttggggccg ccgggcccgg
     3361 cggtcggtgg tcgcaggcat gaagcgcgat ctctcgggac gcgggatgac acaccggtgc
     3421 gaggcacgcc gcggccggga gattttgcca cattttgtgt agcgcagctt cttgtcgttg
     3481 gtggtatgta cagagtgtca gtctgtcgcg ttgattacca ttgccctact gagcgcggag
     3541 cgccggtgcg ctgctccgct accaggagga tccctgacat gacgaaaaaa caagtcgcgc
     3601 tcgaggtcac catgatgctc gcagcgcagc aattggtgga ccggtggttg cggtccccgg
     3661 gtcgagggcc cttccagcgc gcggtttctc gcgagctgcg agagcgctgg aacgacctcc
     3721 ctggcacgga agcgcagcag cgcgcgttcc gtcgcctggc caatgcaatt cgggttggag
     3781 agggacatgc gatcgccaat cccggtcccc tcctgaagat cgcccgccag gccctgcacc
     3841 cctgatcgaa cgcggcagta cgggcaacga tctcgatccc cttcccaact gacgctgccc
     3901 gagctcatcc ggtgacccat gaatcatccc gttcgtaggg cataaaccgg gccgcctgac
     3961 cgtgggctgg tagggtaagt ggcgcgctgg tggttggcca gccacgccgg gccgttccac
     4021 aaataagcgc ccttcctcca gaatcctcgg ctcggctgat gctcgctgat gtcccaagga
     4081 gcccgcccat ggaacctacc ctggtcaacg tgacgttcac gctcaatggg gtcgagcagg
     4141 cgagctcagt cgacgcgcgg cggac
//
LOCUS       DASSGX010000393         2139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_177065, whole genome shotgun sequence.
ACCESSION   DASSGX010000393 DASSGX010000000
VERSION     DASSGX010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177065"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..260)
                     /locus_tag="VFI12_07000"
     CDS             complement(132..260)
                     /locus_tag="VFI12_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056194.1"
                     /translation="MLQFVPVDAEPALPAIDVPLPAIEPAESRDDGRFSLFGDLER"
     gene            complement(839..1621)
                     /locus_tag="VFI12_07005"
     CDS             complement(839..1621)
                     /locus_tag="VFI12_07005"
                     /inference="COORDINATES: protein motif:HMM:NF036563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5343 domain-containing protein"
                     /protein_id="HET7056195.1"
                     /translation="MLEAINPRPSEDERRPYAPAANVLGLLDRIRRRNLPETIDPDFM
                     RIANIPEGSMGRVAATLRFLDLVDEAGRPTDQLRAMARADDDEYRALLGAVVREAYAA
                     DFARVDPAEDTTARIMSAFQRYEPRSQTARMVMLFLGLARAVGIEVLEAPRERQMRDR
                     PTARATPVRTRRDRPQPTTAAPAVDPIRGAIPPLTGSLLFGVTIDDIGNLESQADFET
                     VWSALGLIARARATSLKAARETAEKAAAQRSVDAGEGEVERE"
BASE COUNT          323 a          720 c          741 g          355 t
ORIGIN      
        1 atgcgaagcg cgccccgggc gcggcgatcg acctgaccgt ggaggcgcag ccggtcgcga
       61 cctgcccggc ctgaccgcga tccctttgca cggcgcgctc gcgagcgcgc acgacgccgg
      121 cgcttgggtc gtcagcgctc caggtcgccg aacaggctga accgcccgtc gtcgcgggat
      181 tccgccggct cgatggcggg cagtggcaca tcgatcgccg gcaaggccgg ctcggcatcg
      241 acgggcacga actggagcac cggcaggtcc cgccacgcgg cgcccccggg cagcgcagcc
      301 cgggctggct cgggcccggc ggccggcccg gccccattcg gccgctccgt ccagcagccg
      361 cgaagctccg tgccggtcag tggccggcgg cgtcgatcga gggcacacca gccgacatcg
      421 gcagcgaggg ccgcgcgaaa gtgccggcag gtcgaacagg tggtcgatga gcgacggcaa
      481 acccagcatc tcgaagccgc cgcctgtgcg gcaccgcagt gtgggcacga ccacatcgcc
      541 acgccccgat ggtacctgcg cggagaacgc cgcacttgcc ggtactggct caatctcaag
      601 cgaacgggcg ttcgggtgac gcctcgtgac tgggcgctat gattcccgcc ggagaaagcc
      661 gaaaggcccg ctcgcgcggg ccttccggtt tgctggcatc cgggcacacg ctcggcgggg
      721 ttggttcccg ctcggtggcc ggagccgtgt cgtcacaagc gaacacggga tgcctcgctc
      781 cttcttctgg gccggtggct ggatgcgcgc ctcgcttcct gaaagccccg gcccttatct
      841 attcccgctc gacttcgccc tcccctgcgt caacggatcg ctgggcggcg gccttctcgg
      901 ccgtctcccg cgcagccttg agggaggtgg cccgagcgcg ggcgatcagc ccgagcgccg
      961 accagaccgt ttcgaagtcc gcctgcgact ccaggttgcc aatgtcgtcg atcgtgacgc
     1021 caaagagcag cgatcccgtc agtggtggga tcgcaccccg gatcggatcg acagcgggcg
     1081 cagcggttgt tggctggggg cgatctcgcc tagttcgcac aggggtagct cgggcagtcg
     1141 ggcggtcgcg catttgacgc tcgcgcggag cctctagcac ttcgatgccc acggcgcggg
     1201 cgaggccgag gaacagcatc accatccggg ccgtctggct gcgcggctcg taccgctgga
     1261 aggccgacat gatccgcgcc gtcgtgtcct ctgccggatc gacgcgcgcg aaatccgcgg
     1321 cgtaagcctc gcggaccacc gctccgagga gcgcgcggta ctcgtcgtca tcggcgcggg
     1381 ccatcgcacg cagctggtcg gtcggtcgcc ccgcttcgtc gaccagatca aggaagcgaa
     1441 gcgtggccgc aacacgaccc atgctgcctt ctgggatgtt cgcgatccgc atgaagtccg
     1501 gatcgatcgt ttcgggcaga tttcgacgcc ggatgcggtc gaggagcccg aggacgttcg
     1561 cagctggagc gtaggggcgt cgctcgtcct cgctcggtcg cggattgatg gcttcgagca
     1621 cgattccggg cctccttacg cccgcgagag gatcttgggc cgaatcccaa ggcttgtcaa
     1681 gggcatctgc tctacgtcac gattgagctg gtcacgttgc gcgacgagcg gcggttgcga
     1741 cggtatggtc gtctgaacgt ggtattcggt gcaggtcggg agaagcagaa gatcgtgacc
     1801 atacacaggt ggtgacttta gaccagattc acggtggcgt tgccgccaga gcgcccgaga
     1861 ttggcctggc cggagggtga ggagcgcgcg gtctccaatg tccgcattcc agtcgagatt
     1921 tgggcgcgct gcctcgctcg agagggcatt ctcgagcccg cctctcggac cgtggcatcc
     1981 gcttcgttcc aatggcgggc gcattttcat cgagccgcgc caggaagctg tatcacaata
     2041 ggccatgttg cgcggcgtcg gctgtgcgtc acgcgcgatg gtcagtcgcg atccgtcagg
     2101 accctgggtt cccctcgaat cattgcgcca gccgtactc
//
LOCUS       DASSGX010000394         3413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1595031, whole genome shotgun sequence.
ACCESSION   DASSGX010000394 DASSGX010000000
VERSION     DASSGX010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1595031"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VFI12_07010"
     CDS             <1..262
                     /locus_tag="VFI12_07010"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HET7056196.1"
                     /translation="QYNVPPLHSTDTIEAIGAPREIAKPLLVKPGTPLILMRRLATTH
                     RDAPIELTEEYARPDRLRYRMRLYADPVVDIAAETPTEEQTR"
     gene            259..1407
                     /locus_tag="VFI12_07015"
     CDS             259..1407
                     /locus_tag="VFI12_07015"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET7056197.1"
                     /translation="MTRPLKAAVIGTGFIGPVHIEALRRLGIEVTGILSSTLEHGQTR
                     AAALRLPKAYGSLDELLAEPGLDAVHITTPNYLHKPMVEAAIAAGKHVVCEKPLAMNS
                     EEGQYLLDLAEKAGIVHATNFNFRFYPLCQEARTIVQGGKLGTVYQIYGRYVQDWLLK
                     ETDWNWRLEAEQGGEMRAVADIGSHWLDLTRFIAGQDVTAVCADFATFLPVRKKPLVP
                     LETFAGKELQPDEYEEREIRTEDYASILLHFADGARGVLTVSQVAAGRKNRNEFEING
                     SSGSISWNSERVEEMWIGRRDKASELLLKDASLMSSEARMTTETPGGHAEGFRDTFKM
                     LYKRVYQAIEAGGPPEQPDYPTFADGVYALKLGEAIFESAQQRAWVTV"
     gene            complement(1912..2714)
                     /locus_tag="VFI12_07020"
     CDS             complement(join(1912..2372,2372..2714))
                     /locus_tag="VFI12_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012340188.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HET7056198.1"
                     /translation="MSKLLLPDELWAIIEPLLPSEPPKPRGGRPRVPDRAALTGILFV
                     LRSGIPWELLPPEMGCGSGVTCWRRLRDWQQAGVWDRLHRALLDRLGAADLIDWSRAA
                     LDSASVSAKKGAFAIGPNPTDRGKPGTKRHVVVDRNGIPLAAVLSGANRQDSMLFEPL
                     LDAVPPIKRPSGQRRQRPTKLHADKAYDHPKCRSACRSRHIIPRIARRGIESSERLGR
                     HRWVVERTLAWLNRFRRLTVRYERRADIHHAFLTLGCALICWNFLEKEF"
     gene            2901..>3413
                     /locus_tag="VFI12_07025"
     CDS             2901..>3413
                     /locus_tag="VFI12_07025"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET7056199.1"
                     /translation="MPVSYPKTEAPLIEPGKDDGRGRRLHIPGRNNLLKVGTVLQNRY
                     RVLDIRGVGGMSTVYKARDLRFTSVDRLCAIKEMFNSAEDAKLRQVRLANFQREASLL
                     ATMTHSAIPRIYDYFEQQGTIYLVLELIHGEDLETLLSQNGGPFDQERVVDWAIALCS
                     VIAYLHNQKPE"
BASE COUNT          609 a         1036 c         1194 g          574 t
ORIGIN      
        1 gcagtacaac gtgccaccgc tgcattcgac cgacacgatc gaagcgattg gcgcgccgcg
       61 ggagattgcg aagccgctgc ttgtcaaacc gggaacgccg ctgattttga tgcggcggct
      121 ggcgacgacg caccgggacg cgccgatcga gctcaccgag gagtatgccc ggccggatcg
      181 gttgcgctac cggatgcggc tttacgccga cccggtcgtg gatattgccg cggagacgcc
      241 gacggaggag cagacgagat gacgcgaccg cttaaagccg ccgtgatcgg gaccggattc
      301 atcggtccgg tccacatcga ggcgttgcgc cggctgggga tcgaggtgac cggcatcctc
      361 tcctcgacgc tcgagcacgg gcagacgcgg gccgcggcgc tgcggctgcc gaaggcgtac
      421 gggtcgctcg atgagctgct ggccgagccc gggttggacg cggtccacat cacgacaccg
      481 aactacctgc acaagccgat ggttgaggcg gcgatcgcgg ccgggaagca tgtcgtctgc
      541 gagaagccgc tggcgatgaa ctccgaggag gggcaatacc tgctcgattt ggccgagaag
      601 gcagggatcg tccacgcgac gaacttcaat ttccgcttct acccgctctg ccaggaggcg
      661 cggacgatcg tccagggggg caagctcggc acggtctacc aaatctacgg ccgctacgtg
      721 caggactggc tgctcaagga gaccgattgg aactggcggc tggaggcgga gcaggggggc
      781 gagatgcggg cggtggccga catcggctcg cactggctgg atctgacccg gttcattgcc
      841 gggcaggacg tgacggcggt ctgcgccgat ttcgcgacgt tcctgccggt tcggaagaag
      901 ccgctggtcc cgctcgagac tttcgccggc aaggagctgc agccggacga gtacgaggag
      961 cgcgagatcc ggaccgagga ctacgcgagc atcctgctcc atttcgcgga cggagcgcgg
     1021 ggcgtgctga ccgtctcgca ggtcgcggcc gggcgcaaga atcgcaacga gttcgagatc
     1081 aacggcagct ccgggtcgat ttcgtggaac tcggagcggg tcgaggagat gtggatcggt
     1141 cgccgggaca aggcaagcga gctgctgctc aaggatgcct ccctgatgtc gagcgaggcg
     1201 cggatgacga ccgagacgcc gggcgggcac gccgaggggt tccgcgacac gttcaagatg
     1261 ctctacaaac gggtctacca ggcgattgag gccgggggtc cgccggagca gccggattac
     1321 ccgacctttg ccgacggtgt ctacgcgctg aagctgggcg aggcgatttt cgaaagcgcg
     1381 cagcagcggg cgtgggtgac ggtgtaggtt ttgggtggtc ggttttcgga gttcggtttt
     1441 cggttctcgg ggattgcggc tagccgacgt tgcgcggggt tgccatgccc tgaggtgccc
     1501 gtagaccgcg atggtgttgg gcgatgaacg ggttaatgat ttcgaacgag tcgtgcgaca
     1561 tcgcccggcg ctaaagcacc gggctacatc aaataagccc gctaaagcag gctgaaattg
     1621 agtcggattc aaccggcttc tggtggtcag agggggtaca tggttgttgc cggacaggtc
     1681 cggataccgc acggcccagt cggtttcaac cgacttccga tggtgagacg gggtttcaac
     1741 cccccgcgga gcggcgtatc cggatcaacc cggcaccttc atcaatcccc ggagacgtcg
     1801 gcgaaccggt agcaggagga gcgatcatcg cccttcgggc gattgcgggg cgtggtaacc
     1861 ccgcgctacg tctcgatacc gctctccttt cagcctgctt tagagcacct ttcaaaactc
     1921 cttttcaagg aagttccagc agatcagggc gcagccgagg gtgaggaagg cgtggtggat
     1981 atcggcgcgg cgttcgtagc ggacggtgag gcggcggaag cgattgagcc acgccagcgt
     2041 ccgttccacg acccagcggt gccgaccgag tcgttcgctc gactcgatgc cacgccgggc
     2101 gatgcggggg atgatgtggc ggctccggca ggcgctccgg cacttggggt ggtcgtacgc
     2161 cttgtcggca tggagtttgg ttgggcgctg ccggcgctgc cctgacgggc gcttgatggg
     2221 cggcaccgca tcgaggaggg gctcgaagag catcgagtcc tggcggttgg ccccgctcag
     2281 gaccgcggcg agcggaatcc cgttccggtc gaccacaaca tggcgcttcg tgcccggttt
     2341 gccgcgatcc gtcgggttcg ggccgatggc gacgccccct ttttagcgga gacgctggcg
     2401 ctgtccagtg ccgcccggct ccagtcgatc aggtcggcgg cacccagccg atcgaggagc
     2461 gcgcgatgga gccggtccca gaccccggcc tgctgccagt cgcgcagccg ccgccagcag
     2521 gtgacgcccg aaccgcagcc catctccggc ggcagcagct cccacgggat gccgcttctg
     2581 agcacgaaga gaatgccggt gagggcggcc cggtccggga cgcgcggccg cccgcccctg
     2641 ggcttgggtg gctccgacgg caacagcggt tcaatgatgg cccacaactc gtcgggcaac
     2701 aggagtttgc tcatcctccc actgtgccac gccggcatgg gttttgaaag gcactctaaa
     2761 gccacaagtc ggcccaatgg gccatgcgca tgactatccg atctgattgt cgcttcctag
     2821 aatgaaaccg gtcgtgccga acatgcacat cgcgcagacg catccaggga ccgttcgtgc
     2881 caccgggcaa gaggagcgac gtgccagttt cctatccgaa aaccgaagca ccgctgatcg
     2941 aacccggcaa ggatgacggg cgcggccgcc ggctgcacat tccgggccgg aacaacctgc
     3001 tgaaggtcgg cacggtgctc cagaaccgct accgggtgct ggacatccgc ggcgtcggcg
     3061 gcatgagcac cgtttacaag gcgcgggatc tccggtttac cagcgtggac cggctttgcg
     3121 cgatcaagga gatgttcaac tcggccgagg acgccaagtt gcggcaggtg cggctcgcca
     3181 atttccagcg cgaggcctcg ctgctggcta cgatgacgca ctcggcgatc ccgcggatct
     3241 acgactattt cgagcagcaa gggacgattt acctcgtcct cgagctcatt cacggcgagg
     3301 acctcgagac gctgctcagc cagaacggcg ggccgttcga ccaggagagg gtcgtcgatt
     3361 gggcgatcgc gctgtgctcg gtgatcgcct atctgcacaa ccagaagccg gaa
//
LOCUS       DASSGX010000395         2994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_287077, whole genome shotgun sequence.
ACCESSION   DASSGX010000395 DASSGX010000000
VERSION     DASSGX010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2994
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287077"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="VFI12_07030"
     CDS             <1..645
                     /locus_tag="VFI12_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056200.1"
                     /translation="PEIDPANFTTKIDNPFLPLMPGMRWMYEGEADGVKQVNTVVVTS
                     DTKALMGVTCVAVHDTVEEQGDVTEDTIDWYAQDTDGNVWYFGEDTKELTKGKVTSTE
                     GSWQAGKDGAQPGIAMQAKPKPNGSYRQEYLAGEAEDMAKVIKRGARVSVPFGTYTDV
                     LVTEEWTPLEPGVRERKYYAQEVGMISSKITKGGDEHFELTTFESGKATPEASR"
     gene            1173..1337
                     /locus_tag="VFI12_07035"
     CDS             1173..1337
                     /locus_tag="VFI12_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056201.1"
                     /translation="MSDHEPSAFEAVTRQMVETLAEELREIRHRVDGLLWMGAGAILL
                     DTVLRLAGIR"
     gene            1481..2944
                     /locus_tag="VFI12_07040"
     CDS             1481..2944
                     /locus_tag="VFI12_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056202.1"
                     /translation="MSEQVAAFSPDIVSLSRDSFPRYALRPYQIDPARAITESIRLKQ
                     GKQFVVIFSRQSGKDELLAQILVWTLLRYAETGGTAVVAAPTLRPQGAISRDRLIARM
                     REILPDAVGTREGHIVELGKASARFVSALPTANVRGQTADLLLVANEAQAIQPEVWDP
                     VFDPMAASTNATTLFMGTPWRKDSLLSRQLRHLREVELETGERRVWTVPWTEVAEVLP
                     KYRERVEARIAQFGERHPTIKTEYWLEELDGEEGLFPPHRLAQMQGDHSRRDRAEPGT
                     RYALLIDVAGEDELGGSAEVFASGMRRDCTALTVVEIDAALGRLPLYRVVDRRRWIGT
                     KHTQLRDVLADLARHVWQAERVVIDATGIGAGLASFLAERLQDRALGRAIPVDRFIFT
                     QQSKSQLGWDFVGLIETGRYREYVDPAPIGSAERAVTEAFWAELKGIEFAVAAGPGQA
                     MRWGAAGRGHDDLVMSAALAATLDGFGWERRVARGSE"
BASE COUNT          525 a          873 c         1145 g          451 t
ORIGIN      
        1 cccgaaatcg atccagccaa cttcacgacg aagatcgaca atccgttcct gccgctgatg
       61 ccggggatgc gctggatgta cgagggcgag gcggacggcg tgaaacaggt caacacggtc
      121 gtcgtcacat ccgacacgaa ggcactgatg ggcgtgacct gcgtggctgt gcatgacaca
      181 gtcgaggagc agggtgacgt aaccgaggac acgatcgact ggtatgcgca ggacacggat
      241 ggcaatgtct ggtacttcgg cgaggacacg aaggagctta cgaagggcaa ggtgaccagc
      301 acggagggat cctggcaggc gggcaaggac ggcgcgcagc cgggcatcgc catgcaggcg
      361 aagccgaagc caaacggcag ctaccggcag gaatatctgg ccggtgaggc cgaggacatg
      421 gccaaggtga tcaagcgcgg cgccagggta tccgtgccat tcggcacgta caccgatgtg
      481 ctcgtgaccg aggagtggac gccgttggag ccgggcgtgc gggagcgcaa gtactacgcg
      541 caggaggtgg ggatgatctc ctcgaagatc acgaagggtg gagatgagca tttcgagctg
      601 acgacatttg agtcgggcaa ggcgactccg gaggcgagca ggtgatgcag ggatctgcgg
      661 gggtagatcc gccacttgcc gggacgcggg tttcgggcct gtgaacgggt attccggcat
      721 cgtccgggcg taaacacccg ggctgaatca gatgagcccg ctgacgcagg ctgggatgca
      781 ggtcggccgg cgtagcgccg cccctcccca tggctggcat ggacccctcg cccaaccgcc
      841 cgtcggaggg cagcaacccc gttcgccgga ttggtcccgt cgaccgcgcc ggggaggcac
      901 gatgcggtcc ccgggggccg cgctatgcca tggtggtggg gtctgtaacc gggttcaccg
      961 ggcggcaagg cccgccacgg gggcgggggc tacggtttga cgcggccggg acagcccctg
     1021 tcccggccgt gttatgtctt ccgaaatggg ccgattttgg ggtccgaaaa atcgaaaaat
     1081 caaggctaaa tcgtcacgca gcggggggtc tgcgcggata tgtagataga acatttgttt
     1141 ttacccgcgc tgcggagaga acaggagggg cgatgagcga ccacgaacca tcggcattcg
     1201 aggcggtgac ccgccagatg gtcgagacgc tggccgagga gctgcgcgag atccggcacc
     1261 gggtcgatgg cctgctctgg atgggggccg gcgcgatttt gctcgacacg gtgctgcggc
     1321 tggccgggat cagatagggg cgaaaggcga ggaggcgagc agtcgccggc ccacgtccgg
     1381 aatttgcggg cacagacaag gtgcaggttt tgaaatcgat cggctggtga actgaaacgc
     1441 actggcgagg tgagatgtgg cgagggggaa gcgctcggag ttgtccgagc aagttgccgc
     1501 cttcagcccg gacatcgtct cgctctcccg cgactcgttt ccccgctacg cgctgcgtcc
     1561 ctaccagatc gatccggctc gggcgatcac cgagagcatc cggctcaaac agggcaagca
     1621 gttcgtcgtg atcttcagcc gccagtccgg caaggatgag ctgctggccc agatcctggt
     1681 ctggaccctg ctccgctacg ccgagaccgg cgggacggcg gtggtcgccg cgccgacgct
     1741 gcggccccag ggcgcgatca gccgggaccg attgatcgcc cggatgcggg agatcctgcc
     1801 ggatgcggtc gggacgcggg aggggcacat cgtcgagctg ggcaaggcca gcgcgcggtt
     1861 cgtctcggca ttgccgacgg cgaacgtgcg cgggcagacg gccgacctgc tcctggtcgc
     1921 gaatgaggcg caggcgatcc agcccgaggt ctgggacccc gtgttcgacc cgatggcggc
     1981 cagcacgaac gcgacaaccc tcttcatggg cacgccgtgg cggaaagatt cgctgctctc
     2041 ccggcagctg cggcacttgc gcgaggtcga gctcgaaacc ggtgagcggc gagtctggac
     2101 ggtcccatgg accgaggtgg ccgaggtgct gccgaagtac cgcgagcggg tcgaggcgcg
     2161 gatcgcccag ttcggcgagc gccacccgac gatcaagacc gagtactggc tcgaggagtt
     2221 ggacggcgag gaggggcttt tccctccgca tcggctggcg caaatgcagg gggatcactc
     2281 ccggcgggat cgggccgagc cgggcacgcg gtacgcgctg ctgatcgacg tggccggcga
     2341 ggatgagctc ggcggctcgg ccgaggtctt cgcgtccggc atgcggcggg attgcacggc
     2401 cctgacggtc gtcgagatcg acgccgcgct cgggcggttg ccgctctacc gcgtggtcga
     2461 ccggcggcgc tggattggga cgaagcacac gcaactgcga gatgtgctgg ccgatctggc
     2521 ccggcacgtc tggcaagccg agcgggtcgt gatcgacgcg accgggatcg gggccggatt
     2581 ggcttcgttc ctggccgagc ggttgcagga tcgtgcgctg ggccgggcga tcccggttga
     2641 ccgcttcatc ttcacgcagc agagcaagag ccagctgggc tgggacttcg tcgggctaat
     2701 cgagaccggc cggtatcgcg agtacgttga tccggcgccg atcgggtcgg cggagcgggc
     2761 ggtgaccgag gcgttctggg cggagctgaa ggggatcgag ttcgcggtcg cggcggggcc
     2821 ggggcaggcg atgcgctggg gcgcggcggg gcgaggtcac gacgacctgg tgatgagcgc
     2881 ggcgctggcc gccacgctgg acggcttcgg ctgggagcgg cgcgtggcgc gcggctccga
     2941 gtgaacgaag ggggcgacat gagcgaaggg agcggcatgg acgaccaggc gacg
//
LOCUS       DASSGX010000396         2093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_832003, whole genome shotgun sequence.
ACCESSION   DASSGX010000396 DASSGX010000000
VERSION     DASSGX010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832003"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..545
                     /locus_tag="VFI12_07045"
     CDS             141..545
                     /locus_tag="VFI12_07045"
                     /inference="COORDINATES: protein motif:HMM:NF039679.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6188 family protein"
                     /protein_id="HET7056203.1"
                     /translation="MRELSDRWILPLAGQTVTRCCVDDALNIELSEAGSSEATIQIIG
                     ALDLQLRGRTWRLDPQGDAVALAPALALVGLTVDHAEAFKDGTLNVAFRDGSSFQVAP
                     DRDYEAWVFAGRTGAKAVSLPGGDIAVWRSAA"
     gene            complement(714..2018)
                     /gene="nuoD"
                     /locus_tag="VFI12_07050"
     CDS             complement(714..2018)
                     /gene="nuoD"
                     /locus_tag="VFI12_07050"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HET7056204.1"
                     /translation="MSTETAPPTPESQLAAPYEASAVFSPDASSLGPLTIEADVALER
                     GERLMTLNMGPQHPSTHGVLRVILKLDGELITDCDPVIGYLHRGTEKLGEHHRYAQVI
                     PWTDRTDYVAAPMNNLGCVLAFEKLLGLEAPERAQYWRVIMSELARIASHLVWLGTHA
                     LDIGALSMSLYCFRERELILDLFETFCGARLTTNMMEIGGFSRPIPENLPGMVRDFVK
                     IFPERQQEYADILSANPIWMARTKGVGVIPPEVAINYSLTGACLRGSGVNYDIRKAMP
                     YLVYDRLDFEVPLGQHGDTYDRYLVRMEEMRQTLKILTQALDQCEMMDTPLMAYESEY
                     VIPPHLGTMTTAEDMQRHFVWVIKGISPPVGEAYAAVEHPKGELGYYVVSDGGPLPYR
                     FRIRSPDFVNLASLPYMATGSMLADVVALIGTIDIVLGSVDR"
BASE COUNT          362 a          645 c          721 g          365 t
ORIGIN      
        1 cgcagggcta tctccgctac tacaatagcg ctggccagcc gcttgacgtc tctggacaac
       61 ctggtccgcc cgccttgacc cacattccac tcgactatgc cggcccttgg agcggctggc
      121 ccacctgagc gaggtcaacg atgcgcgaac tctccgatcg ctggattctg ccgctcgccg
      181 gacagacagt gactcgatgc tgcgttgatg acgctctcaa cattgaattg tcggaagccg
      241 gctcttcaga ggcgacgatc cagatcatcg gcgctctaga tctgcagctt aggggcagaa
      301 cctggcgcct ggatccgcag ggagatgcgg tcgcgttggc gcccgcgttg gcattggtcg
      361 gcctgacggt cgatcacgcc gaggcattca aagacggaac actcaacgtg gccttccgcg
      421 acggcagcag cttccaggtt gccccagacc gggactacga ggcctgggtg ttcgcgggaa
      481 ggaccggagc gaaagccgtg tccttgcccg gaggagacat cgccgtctgg cgctcagccg
      541 cgtgatcgcc gccggatgcc agccatgtcg ccgacgacgc gcgggatgcc cctcggctct
      601 ttcgggcgtg ccggacaccg gaacgatggc tggcgccagt ggggcaacgt tggacaacag
      661 cttcgggacg cgggcctctc acgccaattt ccggtagtga caggcgctcc gggttagcga
      721 tcgactgacc cgaggacgat atcgatcgtg ccgatgaggg cgaccacgtc ggcgagcatg
      781 gagccagttg ccatgtaggg gagggacgcc aggttgacga agtctgggct gcggatgcgg
      841 aaccggtagg gcaggggacc gccgtccgag acgacgtagt agcccagttc gcccttggga
      901 tgctcgaccg cggcgtaggc ctcgccgacg ggcgggctga tccccttgat gacccacacg
      961 aagtggcgct gcatgtcctc ggcggtcgtc atggttccca ggtggggcgg gatgacgtac
     1021 tccgactcgt aggccatcag cggggtgtcc atcatctcgc actggtcgag agcctgggtg
     1081 aggatcttca gcgtctgccg catctcctcc atgcggacga gatagcggtc gtaggtgtcg
     1141 ccgtgctggc cgaggggaac ttcgaaatcg aggcggtcgt agaccagata cggcatcgcc
     1201 ttgcgaatgt cgtagttgac gcccgagcca cgcaggcagg cgccggtcag cgaatagttg
     1261 atcgccacct cgggcgggat gacgccgacg cccttggtgc gcgccatcca gatcgggttc
     1321 gcggagagga tatcggcgta ctcctgctgg cgctcgggga agatcttcac gaaatcgcgg
     1381 accatccccg gcaggttttc cgggatcggg cgggagaacc cgccgatctc catcatgttc
     1441 gtcgtcaggc gggcgccgca gaaggtctcg aagagatcga ggatcagctc ccgctcgcgg
     1501 aagcagtaga gcgacatcga gagcgcgcca atgtcgaggg cgtgagtgcc gagccagacc
     1561 aggtgggacg cgatccgcgc cagctccgac atgatcacgc gccagtactg cgcgcgctcc
     1621 ggcgcctcta gcccgaggag tttctcgaag gcgagcacgc agccaaggtt gttcatcggc
     1681 gcggcgacgt agtcggtgcg atcggtccag gggatcacct gggcgtagcg gtgatgctcg
     1741 cccagcttct cggtaccgcg gtgcaggtag ccgatgaccg gatcgcagtc ggtgatcagc
     1801 tcaccgtcga gtttcagaat gacccgcagg acgccgtgtg tgctcgggtg ctgcggtccc
     1861 atattcagcg tcatcagccg ctcgccgcgc tccaacgcaa cgtcggcctc gatggtcagc
     1921 gggccgagtg aggaggcgtc ggggctgaac accgcgctcg cttcatacgg agcagcgagc
     1981 tgggattcgg gagtcggggg cgcggtctca gtactcatga cgctcctcgt ccagtccagc
     2041 gggtgcggac gcggccgacg gtgcgctcgg tgttgtagac cgggaactcg ctc
//
LOCUS       DASSGX010000397         2595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_942759, whole genome shotgun sequence.
ACCESSION   DASSGX010000397 DASSGX010000000
VERSION     DASSGX010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942759"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="VFI12_07055"
     CDS             <1..989
                     /locus_tag="VFI12_07055"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET7056205.1"
                     /translation="QIVADNRTAEANATPGAAAQATPAQTLAGFDQLEAEGRLTPVLT
                     ATKDFYHVSKNFTDPTVSADGWSLEIGGLVSTPKSYTHDELVSRSTTKNITTLCCISN
                     ELGGDLISTAEWTGVPLIDLLKEAGVAQNAVDLKLTASDDYTESIPISIAQDPSVLVV
                     TGMNGEMLPDDHGFPARLIVPPIYGMKNVKWLKKIEPVDNDYMGYWEERGWSDTARYQ
                     IWGRIDYPDGALNAAGPAVSCGMASAGDRGVSRVEVSTDNGATWADAMLEPALNNPFT
                     WVRWAFPFTAQSGQVKMKIRVTDGKGEVMKQEEQSPLPDGATGYPSRTINVK"
     gene            complement(1029..1619)
                     /locus_tag="VFI12_07060"
     CDS             complement(1029..1619)
                     /locus_tag="VFI12_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056206.1"
                     /translation="MSDDYTLSRRSWLPRLNAGQRFTSGTETTPSAPQEPVAVLDEPA
                     PAERSRNNLLVQDWSSALETQFGLTAPEPPHASGRDDSVAVLRDRLAYYEHFDDLIRE
                     NIARSAALFQAVFAEREKARKVQAEAESTMNAVSMEADRRMTAERQHIQHVLMSLMDE
                     ATFLHQRSDALIQRLAEALTELAPVSNEDDEPVAGA"
     gene            complement(1827..>2595)
                     /locus_tag="VFI12_07065"
     CDS             complement(1827..>2595)
                     /locus_tag="VFI12_07065"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7056207.1"
                     /translation="AGRDTFGISYQPDILLARAQKLPVVAVCAIVPRPLQGIMSLTSA
                     GITRPSDLRGKTVGYPGIPSQEAFLTTMLATDGARMEEITLNNVEFNLLPTLISGQAA
                     AVMGAFWTHETIVAEQEGYPVSIMKVEDWGVPLYDELVLVTSEETLATRADMVASALG
                     AIRAGYLAAAADQSQAIDMLSTAYPETDRAVEEQGIALLAPLWTEADPGFGIMRPDDW
                     ARFADWMMEHQLLPAGFELAGTIGANQPTSSPATPTA"
BASE COUNT          447 a          814 c          909 g          425 t
ORIGIN      
        1 agcagatcgt ggccgacaac cggaccgccg aagccaatgc cacgccgggc gcggcggccc
       61 aggcgacgcc ggcgcaaacc ctcgccggat tcgaccagct cgaggctgag gggcgcctga
      121 cgccggtcct gaccgcgacc aaggacttct accacgtctc caagaacttc acggacccga
      181 cggtgagcgc cgatggctgg tccctcgaga tcggcggcct cgtctcgacg ccgaagtcct
      241 acacgcatga tgagctggtc agccgctcga cgacgaagaa catcaccacg ctctgctgca
      301 tctcgaatga gctcggcggc gatctgatca gcacagccga gtggaccggc gtgccgctca
      361 tcgacctgct caaggaagcc ggcgtggccc agaatgcggt cgatctcaag ctgactgcgt
      421 cagacgacta caccgagtcg atcccgatct cgatcgctca ggatccgagc gtgctggtcg
      481 tgaccggcat gaacggcgag atgctgccgg acgaccacgg cttcccggcg cggctgatcg
      541 ttccgcctat ttacgggatg aagaacgtta agtggctcaa gaagatcgag ccggttgaca
      601 acgattacat gggctattgg gaggagcgcg gctggagcga cacggcccgg taccagatct
      661 ggggccggat cgattacccg gacggcgcgc tcaatgcggc gggtccggct gtctcctgtg
      721 gcatggcctc ggcgggagat cgcggcgtat cgcgggtcga ggtcagcacc gacaacggcg
      781 cgacctgggc cgatgcgatg ctcgagccgg cactgaacaa ccccttcacc tgggtccgct
      841 gggccttccc cttcaccgcg caatcgggcc aggtcaagat gaagatccgg gtcacggacg
      901 gcaagggcga ggtcatgaag caggaggagc agtcgcccct ccctgacggc gccaccggct
      961 acccctcgcg cacgatcaac gtgaagtagc gtgtggccag tggccaggat tggagtggcg
     1021 gggctgttct acgcgccggc gacgggttcg tcgtcctcgt tgctgaccgg ggcgagctcg
     1081 gtcagggctt cggcgaggcg ctggatcagg gcatcggaac gctggtgcag gaaggtggcc
     1141 tcgtccatca gcgacatcag gacgtgctgg atgtgctggc gctcggcggt catgcgccga
     1201 tccgcctcca tcgagactgc gttcatggtc gattcggcct ccgcctggac cttgcgtgcc
     1261 ttctcccgct ccgcgaagac ggcctggaag agcgccgccg agcgggcgat gttctcccgg
     1321 atcagatcgt cgaagtgctc gtaataggcc aggcgatccc gcagcacggc aaccgaatcg
     1381 tcgcgcccgg atgcgtgcgg cggctccggc gcggtcaggc cgaactgggt ttcgagggcg
     1441 ctggaccaat cctgcacgag caaattattc cggctccgct cggccggcgc cggctcgtcg
     1501 aggacggcga ccggttcttg cggcgcgctg ggtgtcgttt ccgtgccaga cgtgaagcgc
     1561 tggccggcat taagcctcgg caaccagctc cggcgggaca aggtgtagtc atcagacacg
     1621 gtggtaacag cctccgggga gtgatcagtg cgcgtccggg cccgcgtgga ccggttgtct
     1681 gcgcttgaaa agaggctatt gcccggcgcg gggccggagt atcctccgga agtaccgaag
     1741 cgggccggca tccgaatctc tccgggcaag gaaaaaggga cgggcacgaa catgccagtc
     1801 ccttctcgcg ttggacagag attcgatcag gcggtgggcg ttgccgggct ggaggtcggc
     1861 tggttcgcgc cgatcgtgcc ggccagctcg aagcctgcgg gcaggagctg gtgctccatc
     1921 atccagtcgg cgaagcgggc ccagtcgtcg ggccgcatga taccgaagcc gggatcggcc
     1981 tcggtccaga gcggggccag gagcgcgatt ccctgttcct cgacggcgcg atcggtttcc
     2041 gggtaggcgg tcgacagcat atcgatcgcc tgtgactgat cagccgcggc ggcgagatag
     2101 cccgcgcgga tcgcgccgag cgcgctggcg accatgtcgg cgcgcgtggc gagcgtttct
     2161 tcactcgtga ccagcaccag ctcatcgtac aggggcacgc cccagtcctc gaccttcatg
     2221 atcgagaccg gatacccctc ctgttcggcg acgatcgtct cgtgagtcca aaaagcgccc
     2281 atgacggcgg ccgcctgacc ggagatgagg gtcggcagca ggttgaactc gacgttgttg
     2341 agggtgatct cttccatccg ggccccgtca gtcgcgagca tggtcgtgag gaaggcttcc
     2401 tggctgggaa tgccagggta gccgaccgtc ttgccgcgca gatcggaggg gcgagtgatg
     2461 ccggcgctgg tgagcgacat gatgccctgg agcggccggg gcacgatcgc acagacggcc
     2521 acgacgggca gcttttgcgc gcgcgcgagc aggatgtcag gctggtagga gatgccgaag
     2581 gtgtcgcggc cggcg
//
LOCUS       DASSGX010000398         3163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_723138, whole genome shotgun sequence.
ACCESSION   DASSGX010000398 DASSGX010000000
VERSION     DASSGX010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723138"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..1519)
                     /locus_tag="VFI12_07070"
     CDS             complement(173..1519)
                     /locus_tag="VFI12_07070"
                     /inference="COORDINATES: protein motif:HMM:NF005914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET7056208.1"
                     /translation="MPVEDIRTRAEAMMPDITEDLAALVRIPSCAFPGFPPEPVHEAA
                     EAVVDLLERYGVPNARLLDVPSGYPTVYAEIPAPAGAPTILLYAHYDIQPAPMEQGWD
                     MEPFEPVISEGRLYGRGASDDKSGVMIIASALRLWGGRPPVGVRVLIEGEEETGSNLE
                     SLIAANPDLVRCDAYVINDGGNMRAGEPELTAALRGIAACDVAVRTLKGMAHSGSYGG
                     GAPDALMALIRILDQLLDANGDVAIPGLTRFEWEGGGIPEEEYREAAGVLPGVGLMGT
                     GTLATRLWARPSVTAIGLDAPATDDASNSLIPEAKARVSLRIAPGSDPEESLRRLMQH
                     LHDSAPWHVEVVTAPVNTGDAFMGQTGGPVFAAAREAAAEAFDTEPKVTGSGGTIPLL
                     TTLQRVAPDAEFVIWGPGDQYSQVHAANESLDLVELQRMIVAETLLLEKLGQQAVS"
     gene            complement(1726..2028)
                     /locus_tag="VFI12_07075"
     CDS             complement(1726..2028)
                     /locus_tag="VFI12_07075"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HET7056209.1"
                     /translation="MDEWEELGKLLDDEEPRDYLQRIASNPAVLVGKPVIRGTRISVE
                     LILERLSGNLDLDEFFAAYPHLAAEDVRAALLFARDAVRASYLESEERKDALTAPD"
     gene            2344..2583
                     /locus_tag="VFI12_07080"
     CDS             2344..2583
                     /locus_tag="VFI12_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056210.1"
                     /translation="MSEPAVQFYETSALTHEARINHIERQDEWAIIHDRFNDEWEVWA
                     DGQLYDVSRSSATARQKLHEAMEERPRSGEYRYKR"
     gene            2642..2950
                     /locus_tag="VFI12_07085"
     CDS             2642..2950
                     /locus_tag="VFI12_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056211.1"
                     /translation="MSECPFAMHQMAADTETGPNSESMTIACAICGVGHTFRITWTGH
                     NRARVDTIIFPAEGSHTISREVIRWSIASCEGTVGDMEQDGVIVTRGSTDIQGQPVGL
                     "
BASE COUNT          547 a         1003 c         1085 g          528 t
ORIGIN      
        1 cttcgtgtcc gctccaccgc atcccgcgaa cgccggtacg cagcggagct gcagcagcgt
       61 gcagaaaccg tgacattctg agtgctgtga ccgggtgaaa tcgtctcaat tcgtgtcatg
      121 cttcgctttg ctcggcatga cacgaatttc gcatggcgct ggagcgggtc ggttatgaca
      181 ccgcctgctg gcccagcttc tccagcagca gcgtctcggc gacgatcatg cgctgcagct
      241 cgaccagatc gagactctcg ttagcagcgt ggacctgcga gtactgatcg cccgggcccc
      301 agatgacgaa ctcggcgtcc ggcgccacgc gctgcagcgt ggtcagcagc gggatcgtgc
      361 cgccggagcc ggtcaccttc ggctcggtgt cgaacgcttc ggcggcggct tcccgcgccg
      421 cggcgaagac cggcccgccg gtctggccca tgaaggcgtc gcccgtgttg actggggcgg
      481 tgacgacctc gacgtgccag ggcgcggagt cgtgcaggtg ctgcatcagc cgccgcagcg
      541 actcctccgg gtctgagccg ggcgcgatgc ggagcgagac gcgcgctttg gcctcgggga
      601 tgaggctgtt ggaggcatcg tcggtcgccg gggcgtcgag cccgatcgcg gtcacggagg
      661 gccgcgccca gagccgcgtg gcgagggtgc cggttcccat caggccgacg cccggcagca
      721 cacccgccgc ctcccggtac tcttcctccg ggatgccgcc gccctcccac tcgaaccggg
      781 tcaggccggg gatcgccaca tcgccgttgg catcgaggag ctgatccagg atccggatga
      841 gcgccatcaa cgcgtctggc gcgccgccgc cgtaggagcc ggagtgcgcc atgcccttca
      901 acgtgcgcac cgcgacatcg caggcggcga taccgcgcag ggcggccgtc agctctggct
      961 cgccagcgcg catgttgccg ccgtcgttga tgacataggc gtcgcagcgg acgaggtcgg
     1021 ggttggcggc gatgagggat tccagattgc tcccggtctc ctcctcgccc tcgatcagca
     1081 cccggacccc gacgggaggg cggccgcccc agaggcgcag cgcggaggcg atgatcatga
     1141 cgcccgactt gtcgtcggag gcgccgcggc cgtagagtcg gccctcgctg atgaccggct
     1201 cgaacggctc catgtcccac ccctgctcca tcggcgcggg ctggatgtcg tagtgggcgt
     1261 agaggaggat cgtcggcgca ccggccgggg ccgggatctc ggcgtagacg gtggggtagc
     1321 cgctggggac gtcgagaagg cgcgcattgg gaacgccgta gcgctccagg agatcgacga
     1381 ccgcctcggc cgcctcgtgg accggctccg gggggaaccc ggggaaggcg caagagggga
     1441 tgcggacgag agcagcgaga tcctccgtga tatcgggcat catcgcctcg gcgcgggtac
     1501 gaatgtcttc gacgggcata ctcggacctc cctcatggcg cggattcggt caaccgcggt
     1561 tgtatagcag gatgaacgct cggacggaag ggccgggctt ggcacgtcga acttcgggaa
     1621 cttggggaac ttcgggaact tttccgaggt cacccactca gcacggacta tcggcgatct
     1681 cgtcccgcgg gcgtctcacg ctttggagga cgccaggacc gctattcaat cgggtgccgt
     1741 cagcgcgtcc ttccgctctt ccgattccag atagctggct ctgacggcgt ctcgcgcgaa
     1801 tagaagcgca gcccggacat cctcagcagc gaggtgcgga taggcggcga agaattcgtc
     1861 cagatcgagg ttgccagaca accgctccag gatcagctca acggaaatgc gcgttccccg
     1921 gatgaccggc ttgccgacca ggacggccgg gttactggca atgcgctgca ggtaatcccg
     1981 gggttcctcg tcatcgagca attttcccag ctcttcccac tcatccatct ttggcttcca
     2041 cctcgttagt ttgtgttgtc caagtgtcgc atagccttcg ctgccacaag gcgatgatgg
     2101 ccgaaggcgt caatccagga cgaagagcgg accgggccgg tgccaggttg aggacggcgg
     2161 aattgggtgc ggcacgtttt cggctcggcc gcagaaccat aggctactta tttgactggt
     2221 cgcccaacgt cgctatgctg gcactctgaa ccgacggcgt cggaaccagg cctctgtttg
     2281 gactgccggc gtggatcgtg tcaggcgatg cgcggcgatt ctggaagccg agggagcgcg
     2341 gcgatgtcgg aaccagccgt ccagttttat gagacgagcg cgttaacgca cgaagcgagg
     2401 atcaatcaca tcgagcgtca ggacgaatgg gccatcatcc acgatcggtt caatgacgag
     2461 tgggaggtgt gggcagacgg gcaactctac gacgtgagcc gatcctcggc gaccgcgcgg
     2521 cagaagctgc acgaagccat ggaggagcga ccacgctccg gtgagtaccg gtacaagcgg
     2581 taggggttcc catgcggcgt tccgacggcc caaccgaggt gattgccatg agcgcaccgc
     2641 gatgagcgaa tgcccgttcg ccatgcacca gatggcggca gacactgaaa ccggccccaa
     2701 ctccgagtcg atgacgatcg cctgcgccat ctgcggcgtc gggcacacgt ttcgcatcac
     2761 ctggactggc cacaaccggg cgcgggtcga tacgatcatc ttcccggccg agggaagcca
     2821 caccatcagc cgcgaggtga tccggtggag catcgcctcg tgcgagggca cggtcggcga
     2881 catggaacag gatggggtca tcgtgacccg tggcagcacc gacatccagg ggcaaccagt
     2941 tggcctgtga tcgcgcgacg gcgccgtcac gccctcgcgc ggacggagga cgaacgtggc
     3001 cgacttctac gaatatggtc agcatttccc gccgcgcgac gtcattcatg cccgcgtcag
     3061 atcgaccagg gagacggcgc tcgtgctcga ccgggagtcg ggcttgttgg aggcctggat
     3121 cgacggcaga ctcattgcgc agagcgagag tcagatcgag gtg
//
LOCUS       DASSGX010000399         3155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_942943, whole genome shotgun sequence.
ACCESSION   DASSGX010000399 DASSGX010000000
VERSION     DASSGX010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942943"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..1309
                     /gene="gcvT"
                     /locus_tag="VFI12_07090"
     CDS             164..1309
                     /gene="gcvT"
                     /locus_tag="VFI12_07090"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HET7056212.1"
                     /translation="MTTGNTASDEAPLRETALRGRHEALGARMIPFAGWLMPVHYGGI
                     LDEHRAVRAAAGLFDLGHMGQVRVSGPDALPYLQFVTSNDVSALQPGDAQYALLPTEA
                     GGVIDDIIIYRLPDEPAYFVVVNAANHDSDVAWLLTQRARRADLDVDVSDVSAALGMV
                     AIQGPHAERIVAELTQAELATLGPFQCRRAEVAGVPVLMARTGYTGEDGFEFYVPNDM
                     TGQLWDALLEAGRADGLVPVGLGARDSLRLEARMPLYGNELAEDINPLEAGLGWAVKL
                     DKGPFIGRDAIAAMKASGVPRRTVGFRLLERAGSARHGYPVQRDGQTIGVVTSGAHSP
                     TLGTEIGLALIDAEFAGVGKPLDIVIRGRPIRAEQVRLPFYRRDRAQ"
     gene            1337..1726
                     /gene="gcvH"
                     /locus_tag="VFI12_07095"
     CDS             1337..1726
                     /gene="gcvH"
                     /locus_tag="VFI12_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HET7056213.1"
                     /translation="MPSPTDRKYTKTHEWVSVDGDVATIGITDHAQGELGDITYLELP
                     DVGDAVARDQAFGILESVKAANDIFSPVDGEVIARNEDALASPEMINQSPFDRAWLLR
                     IRVEEPRPLEQLMNSDEYEEFAASAGH"
     gene            1758..3098
                     /gene="gcvPA"
                     /locus_tag="VFI12_07100"
     CDS             1758..3098
                     /gene="gcvPA"
                     /locus_tag="VFI12_07100"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HET7056214.1"
                     /translation="MTFNPHTEDDRAAMLEATGAVDVETFFAPVPAAIRFPRLELPPA
                     LTEMEAADHLTQLAARDVVFPAEDVFLGAGAYRHFVPAAVGQVLARGEFYTAYTPYQP
                     EVAQGTLQVIYEFQSLVAALTGMDVANASIYDGATALAEGALMTVSLPRGRRRIVVSA
                     TVHPAYRAVIQTYLTGLDVEVVELPLPGSGFVTTPEDFAPFLEDDLACIVTQYPNFFG
                     GIEDLPAMADLAHGRGAALVVSTYPVPLALLRPPGELGADIVAAEGQALGIPQSYGGP
                     YVGLLAARQSYVRQMPGRLAGMTADTEGKRGFVLTLQTREQHIRREKATSNICTNQGL
                     MATAATAYMATVGPEGFREVAYRGYQNAHYLADRIAELPAYAVATAEPFFHEFVVTTS
                     VPAREVVAHLLDHGILGGLDLGTVDPSLANAMLLCATETNSRAGIDRLIAALPR"
BASE COUNT          522 a         1044 c         1059 g          530 t
ORIGIN      
        1 gggagcgatc cttgcccggt tagggcttga gaccaattcg tcttgcgtca tagacgacat
       61 atcccaggag attcgctccc gcgattgacg cacttcccaa ccaagcctag aatcggcgcg
      121 gttcgggagc acgtaccaag cgggaaagga cacctggaca acggtgacaa ccggcaacac
      181 agcatccgac gaggcgcccc tgcgggagac ggctctgcgc ggtcgacacg aggcgctggg
      241 agcgcgcatg atcccgttcg ccgggtggct gatgccggtc cactatggcg ggattctgga
      301 cgagcaccgg gcggtacgcg ccgccgccgg gctgttcgat ctcggtcaca tggggcaggt
      361 gcgagtttcc ggccccgatg cgctcccata tttgcagttc gtcacgtcga acgacgtttc
      421 cgcgctccag ccgggcgatg cccagtacgc cttgctgccc actgaggcgg gcggggtcat
      481 cgacgacatc atcatctacc ggctgccgga tgagccggcg tatttcgtcg tggtcaacgc
      541 ggcgaaccat gacagcgatg tcgcctggct cctgacgcaa cgcgcgcgtc gcgccgatct
      601 cgacgtcgat gtttccgatg tctccgccgc gctcggtatg gtggcgattc agggaccaca
      661 cgccgagcgg atcgtcgccg agttgacgca agcggagctg gcgacgctgg ggccgtttca
      721 gtgtcgccgg gcggaggtgg ccggcgtgcc ggtcctgatg gcgcggaccg ggtacaccgg
      781 ggaagacggc ttcgagttct acgttcccaa cgatatgaca ggtcagctct gggatgcgct
      841 actggaggca ggccgggcag atggtctcgt gccggttggg ctcggcgccc gggactcgct
      901 ccggctcgaa gcccgcatgc cgctctacgg gaacgagctg gcggaggaca tcaacccgct
      961 ggaggctggg ctcggctggg ccgtcaaact cgacaagggg ccgttcatcg gccgggacgc
     1021 tatcgccgcg atgaaagcgt ccggcgtgcc gcggcgcacc gtcggcttcc gcctgctgga
     1081 gcgcgctgga tcggcccgac acggctatcc ggtccaacgc gatggacaaa ccatcggcgt
     1141 ggtcacgagc ggcgcccatt cgccgaccct cggcacggag atcgggctgg ccctgatcga
     1201 tgccgagttc gctggcgtcg ggaaaccgct cgatatcgtc attcgaggtc ggcccatacg
     1261 ggccgagcag gtcagacttc cgttctatcg acgagaccgc gcgcaatagc gcgggtgcca
     1321 ggagagagac acgaaaatgc catcgccgac cgatcggaag tacaccaaga cgcacgaatg
     1381 ggtctccgtc gatggtgacg tggcgacgat cggaatcacc gatcatgccc agggtgagct
     1441 gggcgacatc acctatctcg agctgccgga cgtcggcgac gccgtcgcgc gcgatcaggc
     1501 gttcggaatc ctcgaatcgg tcaaagcggc gaacgatatt ttttcgccgg tcgacggcga
     1561 agtgatcgcg cgcaacgagg acgccctggc gtcgccagaa atgatcaacc agtcgccgtt
     1621 cgatcgggcc tggctgttgc ggatccgcgt ggaagaaccg cgcccgctcg agcagctcat
     1681 gaattccgac gagtatgaag aattcgccgc ctcggcgggg cattgatcga tccggttcca
     1741 gggagccacg caagacgatg acattcaacc cacacactga ggacgaccgc gccgcgatgc
     1801 tggaggcgac gggcgcggtc gacgtggaga cgttctttgc gccggtgcca gcggcaattc
     1861 ggttcccccg tctggaactg cccccggcat tgacggagat ggaggccgcc gatcacctca
     1921 cccagctcgc cgcgcgcgac gtcgtcttcc cggccgaaga cgtctttctc ggtgcgggcg
     1981 cgtaccggca tttcgtgccg gcggcggtcg ggcaagtgct cgcgcgtggt gagttctaca
     2041 cggcgtacac cccatatcag cccgaggtgg cgcagggcac gctccaggtg atctacgagt
     2101 tccagagcct ggtcgccgcc ctgaccggca tggacgtcgc caacgcctcg atctacgacg
     2161 gggcgacggc gctggccgag ggcgcgctga tgaccgtctc gctcccccgt gggcggcgcc
     2221 ggattgtcgt ctccgcgacg gtccaccctg cctaccgggc cgtgattcag acctacctca
     2281 ccgggctcga cgtggaagtc gtagagctgc cgttgcctgg ctccgggttt gtcaccactc
     2341 ccgaggactt cgcgccgttt ctggaagacg atctggcctg catcgtcacg cagtacccca
     2401 acttcttcgg cggcatcgaa gacctgccgg ccatggccga cctcgcgcac ggccggggcg
     2461 cggcgctagt ggtgagcacc tacccggtgc cgctcgcgct gctgcggccg cccggggaat
     2521 tgggcgcgga tattgtcgcg gcggaggggc aggcgctggg gatcccgcag agctacggcg
     2581 gcccctatgt cggcctgctc gccgcgcggc aatcctacgt gcgccaaatg ccaggccgtc
     2641 tggccgggat gaccgccgac accgagggca agcgcggatt cgtcctcacc ctgcaaacgc
     2701 gtgagcagca tatccggcgc gagaaggcga cctccaatat ctgcacgaat caggggctga
     2761 tggcgaccgc cgcgaccgcg tatatggcca cagtcggccc ggaagggttc cgcgaagtgg
     2821 cctaccgtgg ctaccagaat gcgcactacc tcgccgaccg catcgcggag ctaccggcgt
     2881 atgcggtggc gaccgcggag ccgtttttcc atgagttcgt ggtcacgacg tccgttcccg
     2941 cgcgagaggt ggtggcgcat ttgcttgacc acgggattct cggcgggctc gatctcggga
     3001 ccgtcgaccc ctcgctcgcc aacgccatgc tgctctgtgc gacggagacg aattcccgcg
     3061 ccggcatcga ccgcctgatc gcggccctgc ctcggtagcc cgccacctct ggcaccgctt
     3121 cccaccgatc ggttgtccgg ttccggccac cgcgg
//
LOCUS       DASSGX010000400         3881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1049604, whole genome shotgun sequence.
ACCESSION   DASSGX010000400 DASSGX010000000
VERSION     DASSGX010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049604"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="VFI12_07105"
     CDS             complement(<1..537)
                     /locus_tag="VFI12_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056215.1"
                     /translation="MIPLGSIIRLQVQVESLKIGDGARQRFDPAGIRTVDQVALDDGG
                     AWGITADGARLADVHHRDHPRSKIRGTLNGISVGFTGHYAAMRGRFGEHLTDGAAAET
                     ILIEHNGIMTAPELANGIIIQNATGQTIHLVEFAIADPCAPFSRHALQFPDDKRPDRT
                     VTEALQFLGDGMRGYYCRY"
     gene            complement(534..1799)
                     /locus_tag="VFI12_07110"
     CDS             complement(534..1799)
                     /locus_tag="VFI12_07110"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005306.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HET7056216.1"
                     /translation="MESQTEQYRSRLFAAIEERTDELVATVAELVREPSVLGNEAGVQ
                     RVVARRLAEAGMRVETYDLPDDTIRQPNGGSSGVPFAGRPNVHGYRTGSSGGRSLILN
                     GHIDVVSPEPVSAWSHDPWAAEIVGDRMYGRGAYDMKSGVGVNLFLARLLNDLDIPLK
                     GDFTIHSVIEEECTGNGALAASLRDTADACLIPESTNYSLTVAHPGVIWFRVRIDGRS
                     AHAGHAWQGVNAIVKAVPVINALAQLNEDLNLQVHPLWADHYHPINLNIGVIQGGDWP
                     STVPGACELHCRTSFFPGTTVEEMQAKIADAVNAAAETDEWLREHRPVVSYDGFRTNG
                     VIVDQDEPLVQLMHSTFSAVTGEKLENKIGTAVTDQRYYNFRGVPSVCFGAQGDNAHA
                     ADEWLDLTSLPVVAKVMGSFILDWCGVAG"
     gene            1869..1939
                     /locus_tag="VFI12_07115"
     tRNA            1869..1939
                     /locus_tag="VFI12_07115"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1901..1903,aa:Cys,seq:gca)
     gene            complement(2000..3013)
                     /locus_tag="VFI12_07120"
     CDS             complement(2000..3013)
                     /locus_tag="VFI12_07120"
                     /inference="COORDINATES: protein motif:HMM:NF005495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7056217.1"
                     /translation="MARPINEQVVVITGASSGIGRETALEFGKHGASVVLAARNEEAL
                     REVASEIEREGGMAHAVTTDVAEWPQVERLAQEAVNRFGRIDTWVNDAAVAEYATVEQ
                     MSIEEIDRILQVNLNGMIYGMKAALPHLKRQDGGTLINVSSVLGARAVPLQAAYTAAK
                     FGVRGFADSLRMELEHDGSHVNVTTILPSSINTPFFDHARSKMGAEPKPIPPVYEPSA
                     VAEAILFAAENSRREIIVGGAGKMMAMMQKISPGFTDRYMLLGGQMFKQQKSDRPNGH
                     KDNLFTPSTGTGSTTGEWGGRMTQSRSLYTHFLGLHPNRGRAALAAGGVLGAVALRRR
                     NAD"
     gene            3364..>3881
                     /locus_tag="VFI12_07125"
     CDS             3364..>3881
                     /locus_tag="VFI12_07125"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7056218.1"
                     /translation="MLKRRVLLVEDHPMYRKGVKRLLEDTGRFQVVGEAINGHDAIHQ
                     ADVHRPEIVLIDVQLPGVTGLKIARVLRKQHQNMKIVFLSMHLDDTRLFDAIRAGAMA
                     YLTKDIDQETLVESLRRVAQGENLINQLILSRPQLAWKVLAEFRQLSGEKAETREQEI
                     AFAALPLSAREI"
BASE COUNT          651 a         1250 c         1265 g          715 t
ORIGIN      
        1 gtagcggcag taataaccgc gcatgccgtc gcccaggaac tggagcgctt cggtgactgt
       61 gcggtccggt cgcttgtcgt ccgggaactg cagggcatgg cggctaaatg gcgcgcaggg
      121 atcagcgatg gcgaactcga ccaggtggat cgtttgcccg gtcgcgttct ggatgatgat
      181 cccgttcgcc agctcgggcg cggtcatgat cccgttgtgc tcgatcagga tggtttcggc
      241 cgccgctcca tcggtcagat gctcgccgaa ccgcccgcgc atggcggcgt aatgcccggt
      301 gaagccaacc gagatgccat tcagcgtccc ccggatcttt gatcggggat gatcgcggtg
      361 gtgaacatcc gccagccgcg ctccatcggc cgtaattccc caggcgccgc cgtcgtccag
      421 cgcgacctga tcgacggtgc ggataccagc cggatcgaac cgctggcgcg cgccgtcgcc
      481 gatcttgagg ctctcgacct ggacctgaag ccggatgatc gacccaagtg ggatcacccg
      541 gcaactccgc accagtcgag gatgaagctg cccatcacct tcgcgacgac tggcagcgag
      601 gtcagatcca gccactcgtc cgcggcgtgg gcgttgtcac cctgcgcacc gaagcagact
      661 gagggaacac cgcggaagtt gtagtagcgc tggtcggtga cggcggtgcc gattttgttc
      721 tccagctttt cgccggtgac cgccgagaag gtcgagtgca tcagctgcac caacggctca
      781 tcttgatcga cgatcacgcc gttggtgcgg aaaccgtcgt agctgacaac cgggcgatgc
      841 tcccgcagcc attcgtcggt ttcagcggcg gcattgaccg cgtcggcgat cttcgcctgc
      901 atctcctcga cggtcgtgcc cgggaagaag gacgtccggc agtgcagctc gcaggcgccc
      961 gggacggtgc tcggccagtc gccaccctga atcacgccaa tgttgaggtt gatcgggtgg
     1021 taatggtcgg cccacagcgg gtggacctgg agattgaggt cttcgttgag ctgcgcgagt
     1081 gcgttgatga ccgggaccgc tttgacgatc gcgttgaccc cctgccaggc gtggccggcg
     1141 tgggctgaac gaccatcgat ccggacccgg aaccagatca cgcccgggtg cgcgacggtc
     1201 agggagtagt tcgtcgactc cgggatcagg caggcatcgg ccgtgtcccg caggctggca
     1261 gcgagcgcgc cgttgccggt gcattcctcc tcgatcacgc tgtggatcgt gaagtcgccc
     1321 ttgagcggga tgtcgaggtc gttgagcagg cgggcgagaa aaagattgac accgacaccg
     1381 ctcttcatat cgtatgcgcc gcgcccatac atgcggtcgc cgacgatctc cgcagcccac
     1441 ggatcatgcg accaggccga gacgggctcc gggctgacca cgtcgatgtg cccgttcagg
     1501 atcagcgatc ggccgccgct ggaaccggtc cggtagccgt gcacgtttgg ccggccggcg
     1561 aaggggacgc cgctggagcc gccgttcggc tgccggatcg tgtcgtccgg gaggtcgtat
     1621 gtctcgacgc gcatgccggc ctcagccagg cgccgggcca cgacgcgctg cacaccggcc
     1681 tcattgccga ggacggatgg ctcccggacg agctccgcga cggttgcgac cagctcatcg
     1741 gtgcgctctt cgatggcggc gaagagacgg gatcggtatt gctcagtctg cgattccaac
     1801 gaatatcccc tgcctgtggt cgacgacgat ttgtcctgcc gggatggtaa acttaaacgc
     1861 tgcaacaggg cgccatggcc aagtggtaag gcaagggtct gcaaaatcct gaccgccggt
     1921 tcgaatccgg ctggcgcctc ccatgaacga aaacgccccg gtccaagcca ggaccggggc
     1981 gtttcacgtc gatgcgggat tagtccgcgt ttcggcgacg gagggcgacc gccccgagga
     2041 cgccgccggc agcgagcgct gcccggcccc ggttcgggtg cagtccgagg aagtgcgtgt
     2101 agaggctgcg cgattgcgtc atccggcctc cccactcgcc ggtcgtcgag cccgtcccgg
     2161 tcgacggcgt gaacagattg tccttgtgcc cgttcggccg gtccgatttc tgctgcttga
     2221 acatctggcc gccgagcagc atgtagcgat cggtgaagcc tgggctgatc ttctgcatca
     2281 tcgccatcat tttgcccgcg ccaccgacga tgatctcgcg gcgcgaattc tcggcggcga
     2341 acaggatcgc ttccgccacc gcgctcggct cgtagaccgg cgggataggc ttgggttcgg
     2401 cgcccatctt cgagcgcgca tggtcgaaga acggcgtgtt gatcgaagag ggcaggatgg
     2461 tggtcacatt gacgtggcta ccgtcgtgtt ccagctccat ccgcagcgag tccgcgaacc
     2521 cgcgcacgcc gaacttggcg gcggtgtacg ccgcctgcag cggcacggcg cgtgcgccaa
     2581 gcacggacga gacgttgatg agcgtgccgc catcctgccg cttgaggtgt ggcagcgccg
     2641 ctttcatgcc gtagatcatg ccgttcaggt tgacctggag gatccggtcg atctcctcaa
     2701 tcgacatctg ctcgacggtc gcatattcgg ccacggccgc gtcgttgacc caggtatcga
     2761 tccggccgaa gcggttgacc gcttcctgcg ccagtcgctc gacctgcggc cattcggcaa
     2821 catcggtggt gacggcgtgc gccatcccgc cttcgcgttc gatctcgctc gcgacctcgc
     2881 gcagggcctc ctcgttgcga gcggccagga cgaccgatgc gccgtgcttg ccgaactcga
     2941 gggcggtctc gcggccgatg ccggatgatg cgccggtgat gacgactact tgctcgttaa
     3001 tcgggcgagc catggcagag ctcctttccg cctgcaatct tgtgcatcat cacccagtgt
     3061 agggaacggc cccgaatcgg caggtgcccg ggcgaccgat gggtcgatct ggtggtgccc
     3121 ggtttatggc tatcgctggt tggggtgtga caggtcggca catagtacct ctcgccgttc
     3181 gcaccttagt actgtccgcc ggtacccagg ggccaaaatg cgctattcca ttcgattctg
     3241 ggtaaaccta ctcttgtccc tggaagagcg agacagttcg ccaccgcccc cggaaccggt
     3301 ccccgccgga tgacgaaaac ccactcgccg ttgtgcatca ataccttggg tagggagttc
     3361 gacatgttga agcgacgcgt tctgctggtt gaggatcacc cgatgtatcg caagggggtc
     3421 aagcgcctcc tcgaggacac gggccggttc caggtcgtcg gcgaggccat caacggccac
     3481 gacgccatcc accaggcgga tgtccatcgt ccggagatcg tcctgatcga cgtccagttg
     3541 ccgggcgtga ccgggctcaa gatcgcccgg gtcctgcgca agcaacacca gaacatgaag
     3601 atcgtcttcc tctccatgca cctcgacgat actcgactct tcgacgcaat ccgggccggc
     3661 gcgatggcct acctgaccaa ggacatcgat caggagacgc tggtcgagtc cctgcggcgg
     3721 gtcgcccagg gcgaaaacct gatcaaccag ttgatcctct cccggccgca gctcgcctgg
     3781 aaggtcctcg ccgagttccg ccagctttcc ggcgagaagg ccgagacgcg ggagcaggag
     3841 atcgccttcg ccgccctgcc gctttcagcc cgcgagatcg a
//
LOCUS       DASSGX010000401         4107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1487518, whole genome shotgun sequence.
ACCESSION   DASSGX010000401 DASSGX010000000
VERSION     DASSGX010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4107
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1487518"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..489)
                     /locus_tag="VFI12_07130"
     CDS             complement(<1..489)
                     /locus_tag="VFI12_07130"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET7056219.1"
                     /translation="MEVSLSPRGRTVVIWVGVIAGAVLLLEAANALRPFAWAIITAYL
                     LHPFVSKIHRRTRLPKQLITLWLYAMIGLVMTILVINFGPVVVDQITQFNDEIPSISN
                     DIENWLNDNQQQRLDDLGIDSQYINDRLDEIGAELGNTLGKAALPVLFSTVSIAIELL
                     IYL"
     gene            739..1773
                     /locus_tag="VFI12_07135"
     CDS             739..1773
                     /locus_tag="VFI12_07135"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HET7056220.1"
                     /translation="MDRPAIHHRGPLLKSLYRSILTRLKEIHRTEHDVLVWPGSGSAG
                     WEIAITNLLCPGDHVVVTVCGDFGERFARAATRLGLVVHRVDKPWGEPVLPTELRGVL
                     ATVPHCQAVLITHNETSTGVTNPLPELATVARDAGALVIVDAVSSAGALPLEVDAWGL
                     DFVISGSQKAWMCPPGLVICAIGPRAWAAYDTSTFPRFFWDIVSAQKSAADGMTPTTP
                     PLPLLFALDAALDLILDEGVEAVWTRHHELGEYARSRVRGIGLQLLADPAFASDSLTA
                     ICVPDELSARSIIDSMMANHRVMLQAGQGEMADRVLRVGHMGWVRQPDLASAFDALAA
                     TLADPGFRVR"
     gene            1924..3552
                     /gene="rpsA"
                     /locus_tag="VFI12_07140"
     CDS             1924..3552
                     /gene="rpsA"
                     /locus_tag="VFI12_07140"
                     /inference="COORDINATES: protein motif:HMM:NF005208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HET7056221.1"
                     /translation="MTDIDSSLMGGADESTQTLSLMEEFLNDPSLEYKSLKYGDVMDG
                     VIMRIDRDEILVDIGSKSEGIVPSREFSSLNSDEKGELNIGDTVLVFVVQPENQEGQA
                     VVSIDRARQEKSWRRLQELYEANESIQAEVTNYNKGGLLVNLDGVRGFVPASQVTEIR
                     GGDEASKQADMAKLIGTKLNLKVIEINRHRNRLILSERQAMQERRDEMKEKLIEELKE
                     GEVRHGRVSSICDFGAFVDIGGADGLVHLSELSWSRVRHPSEVLKVGQEVDVAVLGIN
                     PQERKIALSLKRTQAEPWSQVGDKYEVGQLVRGTITQLANFGAFARIEDGIEGLIHVS
                     ELTDERITHPKQIVKEGDELILRIIRIDPQRRRMGLSLRRALDTPDQELMAVFGEDVL
                     AERDQIVANVNAALEAEGIEPGSGREDEEEEAEEAAPVAQATAPAPDNVQALITESTS
                     GDAPMSAMQAAFAAAGAITEEPAEEVANLESAADNVEQAQEALAEAEEAGAPAAELEV
                     AADAVDSAATELESATAAVRENEESAASEEPTGE"
     gene            3823..>4107
                     /locus_tag="VFI12_07145"
     CDS             3823..>4107
                     /locus_tag="VFI12_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HET7056222.1"
                     /translation="MAQFDKFTERARKVLQLAQEEAQRFNHNYIGTEHLLLGLVREGD
                     GVAARVLNNMGVQLPKVRSAVEFIIGRGEGAVIGDIGLTPRAKKVLELAVD"
BASE COUNT          780 a         1218 c         1365 g          744 t
ORIGIN      
        1 gagatagatc agcagctcga tcgcgatcga gacggtgctg aacaggaccg ggagcgcggc
       61 cttgccgagt gtgttgccaa gctcggcgcc gatctcatcc aaccggtcgt tgatgtactg
      121 gctgtcgatg cccagatcgt cgagtcgctg ctgctggttg tcgttgagcc agttctcgat
      181 gtcgttggag attgatggga tctcgtcgtt gaactgggta atctggtcga cgacgaccgg
      241 tccaaagttg atgacgagga tcgtcatcac caggccgatc atggcgtaga gccagagggt
      301 gatcagctgc ttggggaggc gcgtacggcg atggattttg ctgacgaacg ggtgcagcag
      361 gtacgcggtg ataatcgccc aggcgaaggg acgaagcgca ttggcggctt ccaggagcag
      421 gactgcaccg gcaatgacgc caacccaaat gacgaccgtt cgcccacggg gtgaaaggct
      481 gacttccatt ggtcaactcg ccctgcgagg tgttggctgc ggcgtcacga cctgatcaat
      541 cctgcaattg tccagcgggt tgattttatc agaggtattc aaggacccca cggacgagcg
      601 cccgaggccc ctccgtatac tttggggcga ggcatcgcac acgtgccgat ggaggccagc
      661 ttggattttg accagttcga agacctgaat tttcggctgc ccgggccaac gccgcttccg
      721 ccgtcagtcc gagctgcaat ggaccgaccg gcgatccatc accgagggcc gctgctcaaa
      781 tcgctgtatc gctcaatcct gacgcggctg aaggagatcc accgcaccga gcacgacgtt
      841 ctggtctggc cgggaagcgg gtcggcgggc tgggagatcg cgatcaccaa cctcctctgt
      901 cccggggatc acgtcgtcgt gaccgtctgt ggcgacttcg gcgagcgctt cgcccgggcg
      961 gcgacccggc tcggactcgt ggtgcaccga gtcgacaagc cttggggcga accagtcttg
     1021 cccaccgagt tgagaggcgt gctcgcgacc gtgccgcatt gccaggccgt cctgatcacg
     1081 cacaacgaga cctcaaccgg cgtcacgaat cccctgccgg agctggcgac agttgcgcgg
     1141 gacgccggcg cgttggtgat cgtcgacgcg gtcagctcgg ccggtgcgtt gccactcgag
     1201 gtcgatgcgt ggggactcga tttcgtcatc tccggttcgc agaaggcgtg gatgtgccct
     1261 ccgggactcg tgatctgcgc gatcgggccg cgcgcgtggg ctgcctacga cacatcgacc
     1321 tttcctcgct tcttctggga catcgtcagc gcgcagaaat cggccgccga cgggatgacg
     1381 ccgactacgc cgccacttcc gcttctgttc gcactcgacg ccgcactcga cttgatcctc
     1441 gacgagggcg tcgaggctgt ctggacccgg catcatgagc tcggcgagta tgcacgctcc
     1501 agggtgcgag gcattggact ccagctgttg gccgatcccg cgttcgcatc cgattcgctg
     1561 acggcgatct gcgtgccgga tgagttgtcg gctcggtcaa ttatcgattc gatgatggcg
     1621 aaccaccggg tcatgctcca ggccgggcag ggagaaatgg ccgaccgggt gctccgggtt
     1681 gggcacatgg gatgggtgcg ccagccggac cttgcgtccg cgttcgacgc gctcgcggcg
     1741 acgttggccg accctggctt ccgggttcga tagaagcaac tgcgggttat tgtcatgcgc
     1801 gtgtacccat gggtatactt gctgactgtg tcttttcgcg acgcgcgagt ctaagaacgt
     1861 gttcgccttc tccggcgacg ggctgattcg ggcgcattca cgtgacgggt tacggacctt
     1921 ttgatgacag acattgattc gagtttgatg ggcggagcgg acgagtccac tcagacgctt
     1981 tccctcatgg aagagtttct caacgacccg agccttgagt acaagtcgct caagtacggc
     2041 gatgtcatgg acggggtcat catgcggatc gaccgcgatg agattctcgt cgatatcgga
     2101 tcgaagtctg aaggcattgt cccgtcgcgc gagttttcga gcctgaactc cgatgagaag
     2161 ggcgagctca acatcggcga cactgtgctg gtgttcgtcg tccagccgga gaaccaggaa
     2221 ggccaggcgg tcgtctcgat cgaccgggcg cgccaggaga agagctggcg gcgactgcag
     2281 gagctctacg aggccaacga gtcgatccag gccgaggtca ccaactacaa caagggtggt
     2341 ctgctggtga acctggacgg cgtgcgcggc ttcgtgccgg cgtcgcaggt taccgaaatc
     2401 cgcggtggtg atgaggccag caagcaggcc gacatggcca agctgatcgg caccaagctc
     2461 aacctcaagg tcatcgagat caaccggcat cgcaatcggc tgatcctctc cgagcgccag
     2521 gccatgcagg agcggcgcga cgagatgaag gagaagctga tcgaggagct gaaggagggc
     2581 gaggttcggc acggacgtgt ctcctccatc tgcgacttcg gcgccttcgt cgacatcggc
     2641 ggcgcggacg gactcgtcca cctttccgag ctctcctgga gccgggtccg ccacccaagc
     2701 gaagttctca aggtcggcca ggaagtcgat gtcgcggtcc tcggcatcaa tccgcaggag
     2761 cgcaagatcg cgctctcgct caagcggacc caggctgaac cgtggagcca ggtcggcgac
     2821 aagtacgagg tcggccagct cgttcgcggc acgatcacgc agcttgccaa cttcggcgcc
     2881 ttcgctcgca tcgaggatgg catcgaaggc ctgatccacg tttccgagtt gactgacgag
     2941 cggatcacgc atccgaagca gatcgtgaag gagggcgacg agctcatcct gcgcatcatc
     3001 cggatcgatc cgcagcgccg ccgcatgggc ctcagcctcc gccgcgcgct cgacacgccg
     3061 gatcaggaac tgatggccgt cttcggtgag gatgtcctcg ccgagcgcga ccagatcgtc
     3121 gccaatgtca acgccgcgct cgaggccgag ggcattgagc cgggcagtgg ccgcgaagac
     3181 gaggaagaag aggccgaaga ggccgcccct gtcgcccagg caaccgctcc ggctcccgac
     3241 aatgttcagg cgctgatcac cgagtcgacc agcggcgatg cgccgatgag cgctatgcag
     3301 gcggccttcg cggcggccgg cgcgatcacg gaagagccgg ctgaggaggt cgccaatctc
     3361 gagtcggcgg ccgataacgt cgagcaggcg caggaagcgc tggccgaggc tgaggaggcg
     3421 ggtgcgcccg ctgccgagct cgaggtcgcc gctgacgcgg tggactcggc cgcgaccgag
     3481 cttgagtcgg caaccgccgc cgttcgcgag aatgaagaga gcgccgcgtc cgaagagccg
     3541 accggcgagt aaacaactgc caatgttggc aggttgacca gtccaggctc gaaacgtagc
     3601 gttaatcttg acagttcaat agggcaggag cagcaatgct cctgcccttg ttgattcggg
     3661 gagtagaatt gagcaagagc gaaacgataa gcgcgtagtg tggtcatgga tgcgagagag
     3721 tacgtccggg tactcccgtt tcccgtatgc ttaacgtaga aggtagaaac gcctcgcccg
     3781 tatccgatcc gagagcgttt gcgatttggg agagccgcag taatggcaca gtttgataag
     3841 ttcacagaac gcgcccgcaa ggtgttgcag ctcgcccagg aagaagccca gcgattcaac
     3901 cacaactaca tcggaaccga gcacctcttg ctcgggctgg tgcgggaggg ggacggagtc
     3961 gccgcacggg tcctcaataa catgggcgtg cagctcccga aggtccgctc ggccgtcgag
     4021 ttcatcatcg gccggggcga gggagccgtg atcggcgata tcggtctgac gccgcgcgcc
     4081 aagaaggtgc tcgagctggc agtcgat
//
LOCUS       DASSGX010000402         2852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1595802, whole genome shotgun sequence.
ACCESSION   DASSGX010000402 DASSGX010000000
VERSION     DASSGX010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1595802"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VFI12_07150"
     CDS             <1..243
                     /locus_tag="VFI12_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HET7056223.1"
                     /translation="QDLVLNRATPSEEARRAFYAEATQLDTPDDAEFSTLAGVHGFND
                     AWITEQARRGFIARKADAEGVARISVTSKARTRYGV"
     gene            291..1283
                     /locus_tag="VFI12_07155"
     CDS             291..1283
                     /locus_tag="VFI12_07155"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET7056224.1"
                     /translation="MSRRASRFESIRRIGPWTSRGFVIRASLAVLIIVTALLTNRSFL
                     GWGLFAVFAILIVPTGRIRSFLFSFGPYAGVWFLFTALRSISDETPLARTLNLYAPEF
                     ERELFRGQLPTVTLQDRFFNPDHLHWYDYFCTGIHWSYFIVPHALAIYLWYKRPDLFR
                     RFLGGMILLLGMGLAIYFLIPSNPPWLAPEPINSPSAPVIYRIMQKIGEQIGGGIYQA
                     SYKVIGESNPRAAMPSIHMAITFLLVFPSFRLNRWFGLLMLLYAACMGYSLIYLGEHY
                     FIDVMAGCGIAAYGWYGFPKLSELIQIARQPGAKPILTPAPANQEAAVATPASA"
     gene            complement(1247..1843)
                     /locus_tag="VFI12_07160"
     CDS             complement(1247..1843)
                     /locus_tag="VFI12_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056225.1"
                     /translation="MSPYLIFGLAMLGIVIFSLVVTGNLAAGFNERAKRDMRASLEPL
                     AGQLDGTVSVDEAKVEGRFHGQITTGQVVAGPGGMGRLFETTYIEAAGGSGWRAIVRR
                     PKADGEEWERTFDGPESLSGLRTDVFRLLDALLPYPGWFELKYDPDAGSLMLIRAMQS
                     RRDVPSAERFALYLQTLEAAGLANRTAQAEAGVATAAS"
     gene            2005..>2852
                     /gene="gcvPB"
                     /locus_tag="VFI12_07165"
     CDS             2005..>2852
                     /gene="gcvPB"
                     /locus_tag="VFI12_07165"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HET7056226.1"
                     /translation="MAVEPLIFELSRPGRRGVQFPAADVPTTEIPADLRRERLDWPEV
                     SEIDVVRHFTRVSQKNHAIDINFYPLGSCTMKYNPKINEAVARYSGFASAHPLQDEAT
                     VQGSLELMYDLQQMLATISGFPAVTIQPAAGAQGEFTGVLVMRAYHRARGDEGRNTIL
                     VPDSAHGTNPATAAMAGFKVVTVPSDKRGNVDIDKLRNLVGPETAGLMITNPNTLGLW
                     DENIREAIAVVHEAGGLVYNDGANFNAILGMVRPGDLDIDIMHFNLHKTFSTPHGGGG
                     PGSGPVG"
BASE COUNT          521 a          961 c          836 g          534 t
ORIGIN      
        1 caagatctcg tactcaaccg cgcgacgcca agcgaagaag cccgccgggc gttctacgcc
       61 gaggccacgc agctcgatac gcctgatgac gccgagtttt ccaccttggc cggggtccac
      121 ggcttcaacg acgcctggat caccgagcaa gcccgtcgcg gattcatcgc ccgcaaggcg
      181 gatgccgaag gtgtggcgcg gatcagtgtc acaagcaagg cgcgcacccg ctacggggtg
      241 tagatttgtc gcggtaatcg gcctcgatcc gccggacaag atgtgtacca gtgagccgac
      301 gagcatccag attcgaatca atcaggcgta tcggcccgtg gacatcacgc gggttcgtca
      361 tccgcgcttc cctggcggtc ttgatcatcg tcacggcatt gctgaccaat cgttccttcc
      421 ttggttgggg cctctttgcg gtcttcgcga ttctgatcgt gccgaccggc cggatccgct
      481 cgttcctttt ctcattcggg ccatatgccg gagtctggtt cctcttcacg gcgctgcggt
      541 cgatctccga cgaaacgccg cttgcccgga cgttgaacct ctatgcgccg gaattcgagc
      601 gcgaattgtt ccgcggacag ttgccgaccg tgaccttgca ggatcgcttc ttcaaccccg
      661 atcacctgca ctggtacgac tatttctgca cgggcatcca ctggtcgtac ttcatcgtgc
      721 cgcatgcact ggcgatctac ctctggtaca agcgaccgga tctgtttcgc cgcttcctgg
      781 gcgggatgat cctgttgttg ggcatgggcc tcgcaatcta cttcctgatt ccgtccaatc
      841 cgccctggct ggcgcctgag ccgatcaaca gcccgtccgc gccggtcatc taccggatca
      901 tgcaaaagat cggcgagcaa atcggcggcg gcatctatca agccagctac aaggtgatcg
      961 gcgagtcgaa cccgcgggcc gccatgccct cgatccacat ggcgatcacg ttcctgctcg
     1021 tcttcccgtc gttccggctc aaccgctggt tcggcctgct gatgctgctc tacgcggcct
     1081 gcatggggta ctcgctgatc taccttggtg agcattactt catcgacgtg atggccggct
     1141 gcgggatcgc cgcatacggc tggtacggtt tcccgaagct ttccgaattg atccagatcg
     1201 cgcgtcaacc cggcgccaaa ccgatcctga cgcctgcgcc ggccaatcag gaagcggccg
     1261 tggcgactcc cgcctcggcc tgagcggtac ggttggccag tccggccgct tccaacgttt
     1321 gcaggtaaag cgcgaaccgt tcggcgctcg gcacatcccg gcgcgattgc atcgcccgga
     1381 tcagcatcag cgagccggca tccggatcgt actttagctc gaaccagcct ggatacggca
     1441 ggagcgcatc aagcagccta aagacatcag tccgcagccc ggacagggat tcaggaccgt
     1501 cgaaggttcg ctcccactct tcgccatcgg ccttcggccg gcgcacgatt gcccgccagc
     1561 ccgatccgcc ggccgcttcg atataggtcg tctcgaatag ccgtcccatt ccgccaggtc
     1621 ccgccacgac ctgcccagtg gtgatctgac cgtgaaatcg cccctcgacc ttggcctcgt
     1681 cgacactgac cgtgccgtcg agttggccgg ccagcggctc gagtgacgcc cgcatgtcgc
     1741 gcttggcacg ctcgttgaag ccggccgcga gattaccagt cacgaccagc gagaagatga
     1801 cgataccgag cattgccagc ccgaagatca gatacggcga catgaacccc ttgatcccga
     1861 tcacgcctaa ggacacgcac gacggccaaa tcagccggaa tcgtacgggc tacatcgtat
     1921 ccggcagccg aactcggtac aattccagcg cgtcgtcgcc atgacacgcc tgatccgtct
     1981 cgcatccaca aggggtcccc actgatggcg gtcgagccgc tgattttcga gttgagccgc
     2041 ccaggtcggc gcggcgtgca gtttccggcg gccgacgtgc cgacaaccga gatcccggcc
     2101 gatctgcgcc gcgagcgcct cgactggccc gaggtaagcg agatcgacgt cgttcgccac
     2161 ttcacgcgcg tcagtcagaa aaatcacgcg atcgacatca atttctaccc gctcggctcg
     2221 tgcacgatga aatacaaccc gaagatcaac gaggcggtgg cccgctattc cgggttcgcc
     2281 agcgcccacc cgttgcagga cgaagcgacg gtccagggtt cgctggagct gatgtacgac
     2341 ctgcagcaga tgctcgcgac catttccggt ttcccggcag tcacgatcca gccggcggcc
     2401 ggcgcacaag gcgaattcac cggcgtgctg gtcatgcgtg cctaccaccg ggcgcgcggc
     2461 gatgagggtc ggaatacgat tctcgtgccc gactcggcgc acggcacgaa cccggcaacc
     2521 gcggcgatgg ccggcttcaa ggtcgtcacc gtgccctccg acaagcgcgg caacgtcgac
     2581 atcgacaagc tacggaatct ggttggaccg gagactgccg gtttgatgat caccaacccg
     2641 aatacgctcg gactttggga cgagaacatc cgcgaggcga tcgcggtcgt ccacgaggcc
     2701 ggcggcctgg tctacaacga cggcgcgaac ttcaacgcga tcctcggcat ggtccggccc
     2761 ggcgacctcg acattgacat catgcacttc aatctgcaca agaccttctc gacgccgcac
     2821 ggcggcggtg gtccgggctc aggcccggtg gg
//
LOCUS       DASSGX010000403         4785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1595827, whole genome shotgun sequence.
ACCESSION   DASSGX010000403 DASSGX010000000
VERSION     DASSGX010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4785
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1595827"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1288
                     /locus_tag="VFI12_07170"
     CDS             <1..1288
                     /locus_tag="VFI12_07170"
                     /inference="COORDINATES: protein motif:HMM:NF014868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056227.1"
                     /translation="EIIAFVNERETIEGAPHLEPRHLPVFDCAFKPQNGTRSIHYMGH
                     VRMMGAIQPFISGAISKTVNMPEAATASEIEDVYLEGWKQGLKAIAIYRDNSKRSQPL
                     STGKKKGGDTAEVADAIDSEVVAQLRAQLTKAQAEAGLPHRRRLPSERTAVTHKFDIA
                     GHEGYITVGLYPDGQPGEIFLKMAKEGSTVSGLMDSFATTVSVALQYGVPLRDLVNKF
                     AHVRFEPSGFTGNSEIPIAKSIVDYIFRWLGSRFMSDDDKANLGLIDRSAIVETPPAV
                     VGLSGAATPPPAPAPSASGAVTAAPAAPATPTADNAASETATSSDEPQATAVGTQEST
                     ATEAEHAKALLASEASPVREDLAVIASNGHANGHAANGSSKPLNGGGMTLNLGGVKVA
                     FATQADAPSCMDCGSIMVRNGSCYKCLNCGSTSGCS"
     gene            2000..3388
                     /locus_tag="VFI12_07175"
     CDS             2000..3388
                     /locus_tag="VFI12_07175"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7056228.1"
                     /translation="MIAAGRHNEILRNELLDLSRRPAEFAELQRILQTYFGGQLGQVR
                     FNDLLDQFVTVEYAEPSVNHDLYSVGAGFIQVLQLLSFILGRSPGVILLDEPDAHLHS
                     SLQKTVVDVLDDLSRSRQMQVVLSTHSKEIINYVDPSRLVLIEKGADRAASIGETATQ
                     LTILQSLGEIDNVDAYALVRNRRCLFVEGVSDIAIVERLAARLGLAVFAGDDRVVVIP
                     TSGADRFEHVQQLTVFEGVLGGAIASFEIRDRDGRTEGERTRIQAGAPRPLHILDLDC
                     IESYLINPTVLARVIASVSAERGGSAAPTGDEIEAIAMEIADSMKEEVTDRIADRYAR
                     DRLRETRQYPPAAESNGAARDLVDANWNDLAHRLRVLPGKTFLGRLRERLQADFGVSF
                     GNPRLVEEFAQEEVPAELADLLKQVASQIGGTPPAVGGTAASAAGHTPTPARGASLPG
                     EPSRAPDLTPTE"
     gene            complement(3408..>4785)
                     /locus_tag="VFI12_07180"
     CDS             complement(3408..>4785)
                     /locus_tag="VFI12_07180"
                     /inference="COORDINATES: protein motif:HMM:NF018479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HET7056229.1"
                     /translation="RAPVVGQPGVIASSWSTTSRGRPRPDPRSVPSELLALPCPSGPA
                     CAVSILQRMAPPSPGKVEFKRWLFAPPRPHGALDKDRTVSNLELFYDLVYVAVIGAAA
                     SALKSDISMGGVTIFAIVFGMVWIAWLNGSLYVELHGREDGRTRLLVFLQMGILALLA
                     VFTAHAANGTGTQFALAYAAFLAVMTWQWWRVRELDRTDRPEFLPITAFYVGGMVAST
                     AIVLASAFLPTDVRLIAWTVFVIGWLAGITFAARATRTGQTEGILPTESIVERFGGFT
                     IIVLGEVILGVVAGLAAVEPDALTIVTGLLALVIGFGLWWIYFDFVGRRLPRNTRGTI
                     GAWMLSHLPITASITAAGAAIVSLVEHAHDPTTPAPTAWLLAASVALGLLSLILTSQS
                     LEDAVRLERVYRPLKLALAVGALVALAAGWLALAPWLLALLLVAILSALWFFAVAWLI
                     RAGSWTST"
BASE COUNT          910 a         1546 c         1569 g          760 t
ORIGIN      
        1 cgagatcatc gccttcgtca acgaacggga gacgatcgag ggagccccgc acctggagcc
       61 acgccacctc ccggtcttcg actgcgcgtt caagccgcag aacggcaccc gctcgatcca
      121 ctacatgggc cacgtccgga tgatgggcgc gatccagccg ttcatctcgg gcgcgatcag
      181 caagaccgtg aacatgcccg aggcggccac ggcatccgag attgaagacg tatacctcga
      241 gggctggaag cagggcctga aggccattgc catctaccgg gacaactcga agcgctcgca
      301 gccgctctcg accggcaaga agaagggcgg cgacacggcc gaggtcgccg acgcgatcga
      361 ctccgaagtc gtcgcgcagc tccgcgcgca gctgaccaag gcccaggccg aggcgggtct
      421 cccgcatcga cgccggctcc cctcggagcg cacggccgtc acccacaagt tcgacatcgc
      481 cggccacgag ggctacatca ccgtcgggct ctatcccgac ggccagcccg gcgagatctt
      541 cctgaagatg gccaaggaag gctcgacggt gtcgggcctc atggacagtt tcgctacgac
      601 cgtgagcgtc gcactccagt acggcgtgcc gcttcgcgac ctggtgaaca agttcgcgca
      661 cgtccggttc gagccctcag gcttcaccgg gaacagcgaa atcccgatcg ccaagtcgat
      721 cgtcgactac atcttccggt ggctcggcag ccgcttcatg agcgatgacg acaaggccaa
      781 cctggggctc atcgatcgca gcgccatcgt cgagacaccc ccggcggtgg tgggcctgtc
      841 cggtgcggcg acaccgccgc cggcccctgc gccttcggct agtggggccg tgacggcggc
      901 gccagccgcc cccgccaccc ccaccgccga caacgcagcc tcggagacgg ccacgtcaag
      961 cgatgagcct caggccaccg ctgtcggtac tcaggagtcc acggccaccg aggccgagca
     1021 cgccaaggca cttctcgcca gcgaggcttc tcctgtcagg gaggacttgg ctgtcattgc
     1081 cagcaatggg catgccaatg ggcatgctgc caatgggtcg tcgaagccgc tcaatggcgg
     1141 tgggatgacg ctgaacctcg gtggcgtgaa ggtggccttc gcgacccagg ccgacgcccc
     1201 gtcctgcatg gactgcggct cgatcatggt tcgcaacggc tcttgctaca agtgcctgaa
     1261 ctgcgggagc acgagcggct gcagctgagc tgggtcctag cggagtactt tgagccccgg
     1321 agacatccgg ggctcaactg tttgcgccgc caactgctgg cgagagaggt cgggtggctg
     1381 acaaatgccc gaggtagcga ctggccaact tacgctcgcc gaggcgatga cgggcgaaga
     1441 gcccttcctg ctcgacgatg aatcgccctc gccggcagcc cctccgcccg aacgtactcc
     1501 gagcggaccg gccacgagtc gtatcgcgaa actctgggtc gaaaacttca aaggattcca
     1561 aaggttcgaa gtcgcgctcg gcgacttcaa cgtgttggcg ggcccaaaca atgcgggcaa
     1621 gtcgacgctg ctgcaagcgg cagagcttgt attccgcctc gttgacctcc atcggcaggg
     1681 caaccacctt gcctccggcc gcaacgtgcc tgccgcgatc ctgcccgtgg cgctgctgcg
     1741 cgacctgtgg aaccgccgcc gctatcgcgc tggcaatgcc ttcgttcaag cggtcgtcgg
     1801 cgccgagttc caggatggct cgcttgttga attcgggatc atcggcccat tcggtgccgt
     1861 gaactcgcgc gttcaacgat ccgtcgggtt tgatgaggaa cggctggaca tgcttgccgc
     1921 caggccagcc gtctgggtgc caagcagcat cgggatcgtt cgggatgagg agtatcggcc
     1981 gcctgcccgc cgacagggaa tgatcgccgc cggccgacac aacgagattc ttcgcaacga
     2041 gttgctcgat ctctcgcgac gtccggcaga gttcgccgag ctccagcgga tcctgcagac
     2101 ctactttggt ggccagctcg gccaggtccg gttcaacgac ctgctggatc aattcgtaac
     2161 cgtcgaatat gcggagccgt cggttaacca cgacctgtac tccgtcggtg cgggcttcat
     2221 ccaggtcctg caactgctct cattcattct cggccgcagc cctggggtca tcctgctgga
     2281 cgagccggat gcacatctgc acagcagtct gcagaagact gtggtcgacg tccttgatga
     2341 cctatcccga tcgcggcaga tgcaggtcgt cctctcgacg cactcgaagg agatcatcaa
     2401 ctacgtagac ccttctcgcc ttgtcttaat tgaaaaaggc gccgatcgag ccgcgtcgat
     2461 cggtgaaacg gcaacgcagc tgacgatcct ccagtctctc ggggagatcg acaacgtcga
     2521 tgcgtacgcc ctggtgagga atcgtcgatg cctgttcgtc gagggagtct ctgacattgc
     2581 gatcgtcgag cgccttgcgg cccgcttggg actggccgtc tttgccggcg acgaccgggt
     2641 tgtcgtaatt ccaacaagcg gggcagaccg cttcgagcac gtccaacagt taactgtgtt
     2701 cgagggagtg cttggcggcg ctatcgcttc gttcgagatt cgggatcgcg atggaagaac
     2761 cgagggagaa agaaccagga ttcaggcagg ggctccgcgg cccctacata tcctcgactt
     2821 ggactgcatt gagagctacc tgataaatcc cacggtcctc gcacgtgtga tcgcaagcgt
     2881 cagcgctgag cgaggcgggt ccgcggcacc aaccggggac gagatcgaag cgatcgccat
     2941 ggaaatcgcc gactcaatga aggaagaagt aacggatcgg atcgccgatc gatacgctcg
     3001 tgatcggctt cgagaaacga ggcaatatcc gcccgctgcg gaatcgaacg gagctgcgcg
     3061 agacctagtt gacgccaact ggaacgatct agctcacaga ctccgagtgc tcccaggcaa
     3121 gacgttcctt ggacgcctgc gcgaacggct tcaggcggat ttcggcgtca gcttcgggaa
     3181 tccacgtctc gttgaggagt tcgcccaaga ggaggtcccg gccgaactcg ctgatctact
     3241 taagcaagtc gctagccaga tcggtggaac cccaccggcg gttgggggga ctgctgcttc
     3301 agccgccggg catacgccca cccccgcccg cggtgcgtca ttgcctggtg aaccttcgcg
     3361 tgcccctgac ctgactccga ctgagtaatt accggtggga cagaactcta tgtcgaggtc
     3421 cacgatcccg cgcggatgag ccacgcgacg gcgaagaacc acagcgctga caggatcgcc
     3481 acgaggagca gggccaggag ccatggggcg agcgcgagcc aaccggcggc tagggcgacc
     3541 agcgcgccca ctgcgagggc aagcttgagc gggcggtaga cacgctcgag gcgcacggcg
     3601 tcctcgagag attgcgacgt caggatcagg gacagcaggc caagggcgac cgaggcggcg
     3661 aggagccagg cagtcggggc gggcgtcgtc gggtcgtggg cgtgctcgac gaggctgacg
     3721 atcgcggcgc cggcggcggt gatcgaggcc gtgatcggca ggtggctgag catccaggcg
     3781 ccgatcgtgc cgcgggtgtt gcggggcaat cgacgcccaa cgaagtcgaa gtagatccac
     3841 cacagcccga agccgatgac gagcgcaagc aggccggtca cgatcgtcag tgcgtcgggc
     3901 tcgacggcgg caagaccggc gacgacgccc aggatcacct cgccgaggac gatgatcgtg
     3961 aagccgccga agcgctcgac gatcgactcg gtcgggagga tgccctcggt ttggccggtt
     4021 cgggtcgcgc gggccgcgaa cgtgatcccg gcgagccagc cgatcacgaa gacggtccag
     4081 gcgatgagcc ggacatcggt cggcaggaag gccgaggcga ggacgatcgc ggtcgaggcg
     4141 accatcccgc cgacgtagaa ggcggtgatg ggcaggaatt cgggtcgatc ggttcgatcg
     4201 agctcgcgga cgcgccacca ctgccacgtc atgaccgcga ggaacgccgc gtaagcgagc
     4261 gcgaattggg tgccggtacc gttcgccgcg tgggccgtga acacggccag cagcgccagg
     4321 atccccatct ggaggaatac gagcagacgc gttcggccgt cctcgcgccc gtggagctcg
     4381 acgtacagcg agccgttgag ccacgcgatc cagaccatcc cgaagacgat cgcgaagatc
     4441 gtgacgccgc ccatggagat gtccgacttc agcgccgacg cggccgcccc gatgaccgcg
     4501 acatacacga ggtcgtagaa cagttcgagg ttgctgacgg tccggtcctt gtcgagcgcc
     4561 ccgtgcggcc gcggcggcgc aaagagccag cgcttgaact cgaccttgcc ggggctcggg
     4621 ggcgccatcc gctggaggat actgaccgcg caagcaggcc cggacggaca tggcagggct
     4681 agcagctcgc tcggcaccga gcgcggatca ggccgcggcc gtccacgcga tgtggtcgac
     4741 cacgagctcg cgatcacacc cggctgcccc acaaccggtg cccgt
//
LOCUS       DASSGX010000404         5186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_178153, whole genome shotgun sequence.
ACCESSION   DASSGX010000404 DASSGX010000000
VERSION     DASSGX010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5186
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178153"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2039
                     /locus_tag="VFI12_07185"
     CDS             <1..2039
                     /locus_tag="VFI12_07185"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET7056230.1"
                     /translation="LERLRRVLGSKQVLLMLDNFEHVVEAAPFVVDLLTAFSGVKMLV
                     TSRVTLRVSGEHEFPVEPLPVPNPERLPPLAELAGFGAVALFDQRARAARPDFALDEA
                     NAAAVAEVCARLDGLPLAIELAAARSKVLSPQAMLTRLEHRLALLSGGGRDQPERLRA
                     LREAIAWSYDLLTPHQQALFRQLAVFAGGCTAEAAAFVAGGDAGPASQVLDDLSSLVD
                     SSLLVQRQQRDGEPRFSILETIREYGLERLASAGEEETTRRRHAEWCVSMADEFWPTL
                     KHGLEPAQVIGRLGPEHANLRAALMWLDQTGDREAVLRLAGAISLYWYVHEDLREGLA
                     WLECALDDAAEMPADIRARALLGAGMLSHYVTDDVRAVPWAEASLALYRTVDDAWGMA
                     FALLLLGIVSEDAGNYDQAASYFSESLVRARAAENRVLTGLSLIHLGVVAWGQGDRER
                     ATRWVIDALEVQRANGNVIYGAAESLAFLGLLACESGDLARSVELHRESLSLHVERGY
                     IEVLAVNLADVAILALAAGRPERAVRLFGAAVAQRDAIGNPFKLPERDVYERGTAAAR
                     EQLGDRFAAAWNAGRACSLADAVADAFATLDEFDGLATSTSISAPPGIEASLTSRELE
                     VLRLLVAGGSNREIADALFVSHRTVQAHVANLFAKLDVHSRAAAVSRAYELGLV"
     gene            2383..2847
                     /locus_tag="VFI12_07190"
     CDS             2383..2847
                     /locus_tag="VFI12_07190"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HET7056231.1"
                     /translation="MGAGMPEGNESVVRRYLDEVVNAGSTDLLAGLIATNHIGHDPLG
                     DHYGPEGMRIAVAEYRAAFSDLRVTAEDFVTEADKVVHRFTLRGTHTGPFMGIPPTGR
                     VVTVSGIAIDRLADGKVAESWVNLDALNLLRQLGAPPTLERGPSGPPDDHDR"
     gene            2935..4068
                     /locus_tag="VFI12_07195"
     CDS             2935..4068
                     /locus_tag="VFI12_07195"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET7056232.1"
                     /translation="MLAFVALGPATAVIARATAATPSPVAAPTTWDELLQAGLDRGLT
                     SVALRVEQGSTLLFEGAAGLASRENQTPVAATDRFRIASITKTFTAVLVLQQVDAGLL
                     RLDDTISKWLDDPVVAQIPNVDQITIRQLLSHTSGVYDYFDQDSPFWQDAYFGEGADW
                     TRVWTPLELLAYVGADKHAPYFAPGKGAHYSNTGYILLGLILEKATDQHYVDLLHAGI
                     IHPVGLTDTYFAAAEPVPGGMVDAYHLIAGDLVNVSTIDLSALWTAAAMVSTTKDLDL
                     FLDALLGGELVQPATLDEMLTFSAMDHPGFDGGLGVARWQTAGGAAIGHSGDGPGSAG
                     RMYRLADTDLTIVMLTNTGGDDEATDTTYTDAVQMALATAANS"
     gene            4172..>5186
                     /locus_tag="VFI12_07200"
     CDS             4172..>5186
                     /locus_tag="VFI12_07200"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET7056233.1"
                     /translation="MFSTARLARFSARHPWYIVAIWCVLLALAVGAATGLGDALTNEG
                     DFTNRPESVRADDLLKDRLRGGQDQPVTETVIVHSDSATVDDAAFRQTVEQTAVRLRA
                     LPAVVSNVTTFYEADAAGAPESAQLVSADRHTALMPVTLVGDIDAASEHVDEYLAAVA
                     VQNREGIQVLTVGDISVGDEFNRLAESDLIKGEGIGLLAALIILVVVFGALVVAGVPL
                     VLAIASIIVAIGLTAMLGRVMDLSFFIVNMITMIGLAVGIDYSLFIVERYREERRHGR
                     PKLQAIEITGGTATKAVVFSGTTVVLALLGMFLIPTVIFRSLGAGAILVVIVAVFATL
                     TLVP"
BASE COUNT          831 a         1730 c         1707 g          918 t
ORIGIN      
        1 cactggagcg cctgcggcgg gttctcgggt caaaacaggt gctgctgatg ctcgacaact
       61 tcgagcacgt cgttgaggcc gccccgttcg tcgtcgacct gctcaccgcc ttcagcggag
      121 tcaagatgct ggtcaccagt cgcgtgacgc tgcgtgtctc gggcgagcac gagttcccgg
      181 tcgagccgtt gccggttccc aacccggagc gccttccgcc cctcgccgag ctggccggat
      241 tcggggcggt tgccctgttc gaccagcgtg cccgggctgc ccggccggat ttcgcgctcg
      301 atgaggctaa cgctgccgcg gtggcggagg tgtgcgcgcg gctggacggg cttccgctgg
      361 cgatcgagct ggccgccgcc cggtcgaagg tgctctcacc ccaggcgatg ctcacgcgcc
      421 tcgagcaccg cctggccctg ctgtccggcg gcggtcgaga ccagccggaa cgcctgcgcg
      481 cactgcgaga ggctattgcc tggagctacg acctgctcac gccgcatcag caggcgctgt
      541 tccggcagtt ggccgtgttc gccggtggct gcactgcgga ggcagcagcg ttcgttgccg
      601 ggggagatgc cggcccggcg tcacaggttc tggacgacct ttcgtccctg gtagatagca
      661 gcttgctggt ccagcggcag cagcgggacg gcgagccgcg attttcgata ctggaaacga
      721 tccgcgagta cgggctggag cggctggcat cggccggtga ggaggagacg acgcgtcgcc
      781 gacatgcgga gtggtgcgtg tcgatggccg acgagttctg gccgaccctc aagcacggtc
      841 tggaacccgc acaggtcatt ggtcgccttg gcccggagca cgctaacttg cgggccgcct
      901 tgatgtggtt ggaccagacc ggcgatcggg aagctgtcct gcggctggcg ggcgccatct
      961 ccctttattg gtatgtccac gaagatcttc gcgaggggct ggcatggctc gagtgcgcgt
     1021 tagacgacgc ggccgagatg ccggccgaca tccgggcacg agcgctgctc ggggccggaa
     1081 tgctctcgca ctatgtgacc gacgacgtcc gcgccgtgcc gtgggccgag gcgagcctgg
     1141 ccctgtaccg gacggttgac gacgcgtggg gcatggcctt cgccctcctg ctcctcggaa
     1201 tcgtttccga agacgccggc aactatgacc aggcggcgtc gtacttctcc gagagcctgg
     1261 ttcgtgccag ggcggcagaa aaccgggttc tgaccggact ctcactcatc caccttggcg
     1321 tcgtcgcgtg gggacaaggc gaccgcgagc gggcgacgcg ctgggtaatc gatgcgctcg
     1381 aggtgcaacg ggcgaacggt aacgtcatct atggcgccgc cgaatcgctc gcctttctcg
     1441 gcttgctcgc ttgcgaatcg ggtgatctcg cgcgatcggt cgagctacac cgcgagagcc
     1501 tgtcactcca cgtcgagagg ggctacatcg aggtgctggc ggtcaacctg gccgacgtgg
     1561 cgatactggc cctggccgcc ggccgtccgg agcgcgccgt tcgcctgttc ggggcggcgg
     1621 tggcgcagcg ggacgcgatc ggcaacccgt tcaagcttcc ggaacgcgat gtctacgagc
     1681 ggggcacggc ggcggcccgg gagcaactcg gagaccggtt tgcggcggcc tggaacgccg
     1741 gccgcgcctg ttcgctggcg gatgccgtgg ccgacgcatt tgccacgctc gatgagttcg
     1801 acgggctggc gacgtccact tcgatatcgg ccccacccgg aattgaggcc agtctcacca
     1861 gtcgcgaact cgaggtactg cgtctgctcg tggctggtgg ctccaaccgg gagattgccg
     1921 atgcgctctt cgtcagccat cgcaccgtcc aggcccatgt ggcgaatctt tttgccaagc
     1981 tggacgtcca cagccgcgcc gccgccgtct cccgcgccta cgagctcggc ctggtctgac
     2041 gccccgcccc cgcaacgctt tctcgatcac ctcaaaacgg agtgcggggg taccacgccc
     2101 cgctatcgcc cgcagggcga tgatcgttca catgcggacg gttcccggtg ctcaaacccg
     2161 ttcctcagga agaccatcgg ggcctgcggc cacagcgggg cacgatgacc ccgcgctccg
     2221 tcaatgagcg gacctcggca ggcctgccgt acccaatcta ggtaatgcga ataggccatt
     2281 cggcgaatgt gcccgggcag tgtgctgcct agggtaggac tcgcggcagg cgccgccacc
     2341 ccgagacgtt cccggggcag ccgacgtcca gcaggagccg aaatgggagc agggatgccc
     2401 gaagggaacg agtccgtcgt gcggcggtac ctcgacgaag tggtcaacgc cggcagcacc
     2461 gacctcttgg ccggtctcat cgcgaccaac cacatcggcc acgatccgct cggtgatcac
     2521 tacggaccgg aaggcatgcg gattgccgtg gcggagtatc gcgccgcctt ttctgacctc
     2581 cgggtgaccg ctgaggactt cgtcacggaa gcggacaagg tcgtgcatcg cttcaccctc
     2641 cggggaaccc acaccggccc gttcatgggc attccgccca ccggacgggt ggtgacggtc
     2701 tccggcatcg cgatcgaccg cctcgctgac ggcaaggtgg ccgagagctg ggtgaatctc
     2761 gacgccctga acctgctgcg ccaactcggt gcgccgccga cactcgagcg cggaccatcg
     2821 ggtccgccgg acgaccacga tcgatgaccg ccggttgccg gccattgaat tgaaagggaa
     2881 agcacttcca tgcaatgcac gagacgacaa tttgttctca tcccggcccc agcgatgctg
     2941 gcgttcgtag cgcttggccc agccacggct gtgatcgccc gggccaccgc cgcgacgcca
     3001 tcgccggtcg cggcgcccac tacctgggac gaactcctcc aggccggact cgaccggggc
     3061 ctgaccagcg tcgccctccg ggtcgagcag ggcagtaccc tcctgtttga gggagctgcc
     3121 gggctcgcca gccgcgagaa ccagactccg gtagcggcga cagaccggtt ccgcatcgcc
     3181 agcatcacca agaccttcac tgccgtgctc gtcctgcaac aggtcgatgc gggcctcctg
     3241 aggctcgacg acacgatcag caaatggctc gacgatcccg tcgtcgcaca aattccgaac
     3301 gtcgaccaga tcaccattcg ccagcttctg agccacacga gcggggtgta cgactacttc
     3361 gaccaggaca gcccgttctg gcaggacgcc tacttcggcg agggcgcgga ctggacacgg
     3421 gtctggacac cgctggagtt gctggcgtac gtcggggccg ataagcacgc gccctacttt
     3481 gccccgggca aaggcgccca ctattccaac accgggtaca tcctgctcgg cctgatcctt
     3541 gagaaggcca ccgatcaaca ctatgtcgac ctgctgcatg ccggaattat ccacccggtc
     3601 gggctgaccg acacctactt tgccgcggcc gagccggtac ctggcggcat ggtggacgcc
     3661 tatcacctga tcgccggaga cttggtcaat gtcagcacca tcgacctgtc ggcgctatgg
     3721 acggccgcgg caatggtctc tacgacgaag gacctggacc tgtttctcga tgcgctcctc
     3781 ggcggtgagc tggtgcagcc ggcaacgctc gatgagatgt tgacattctc cgcaatggac
     3841 cacccgggct ttgacggcgg cctgggcgtg gcccgctggc aaacggcggg cggcgccgcc
     3901 atcggtcaca gcggtgatgg cccaggctcc gccggtcgta tgtaccggct tgccgacacg
     3961 gacctcacca tcgtgatgct caccaacaca ggtggcgacg atgaggcaac tgataccacc
     4021 tataccgatg cggtgcagat ggcactggca acggcggcga acagctaact gtcccggccc
     4081 gggacgttcc gtcccgggcc gaccgatccc gatgcttggc gactccccgg ggaggggagc
     4141 gcatccgttg attcaacagg agcatccggc catgttctct actgcccgac ttgcgcggtt
     4201 cagcgcccga catccctggt acatcgtcgc gatctggtgc gttctccttg ctctcgcggt
     4261 aggcgccgcc accggtctcg gcgacgcgtt gacgaacgag ggcgacttca ccaatcggcc
     4321 ggagtcggtc cgcgccgacg acctgctcaa ggaccggctg cgcgggggcc aggatcaacc
     4381 ggtgaccgaa accgtgatcg tccactccga ctcggccact gtcgacgacg ccgcgttccg
     4441 gcagaccgtg gaacagaccg cggtcaggct gcgcgcgctg ccggccgttg tctccaatgt
     4501 cactacgttt tacgaggccg acgcggccgg agctcccgag tcggcccagc tcgtttccgc
     4561 cgaccgccac acggcgctga tgccggtcac gctggtcggc gatattgatg ccgccagcga
     4621 gcatgtcgat gagtatctgg ctgccgtcgc ggtccagaat cgggagggga tccaggtcct
     4681 gaccgtcggt gacatctccg tcggggatga gttcaacagg ttggccgaaa gtgacctgat
     4741 caagggcgaa ggcattggcc tgcttgcggc cctcatcatc ctggtcgtcg tcttcggggc
     4801 attggtcgtg gcgggcgttc cgctcgtgct cgctatcgcg tcaatcatcg tcgccattgg
     4861 cctgaccgcc atgctcggcc gggtgatgga tctctccttc ttcatcgtta acatgatcac
     4921 gatgatcggc ctggcggttg ggatcgacta ctccctgttc attgtcgagc gctaccgcga
     4981 ggagcggcgc cacggccggc cgaagctcca ggcgatcgag atcaccggcg gcactgcaac
     5041 caaggcagtg gtcttctccg gcacgaccgt cgtgctggcg ttgctgggaa tgttcctgat
     5101 cccgaccgtg attttccgta gcctcggcgc gggagcgatt ctggtcgtga ttgtcgcggt
     5161 attcgccacc ctgactttgg tgccgg
//
LOCUS       DASSGX010000405         2486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_71115, whole genome shotgun sequence.
ACCESSION   DASSGX010000405 DASSGX010000000
VERSION     DASSGX010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71115"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..309
                     /locus_tag="VFI12_07205"
     CDS             55..309
                     /locus_tag="VFI12_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056234.1"
                     /translation="MKNKDVMLGSLRLNLNATRAGVSISYYDQDFRHHQGMMKVKIGY
                     LTPDELRSGEVDTGIDITPILPVAAELFTAAGGTLDEDAA"
     gene            complement(362..613)
                     /locus_tag="VFI12_07210"
     CDS             complement(362..613)
                     /locus_tag="VFI12_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056235.1"
                     /translation="MEPTWLLAMVMSAVVSRRWAIPVVAVACAIFTGLRFYDSYGMPL
                     LFGTIGALAGLPLRWGIEELIDETREVFAAIIPHRHDPR"
     gene            863..1756
                     /locus_tag="VFI12_07215"
     CDS             863..1756
                     /locus_tag="VFI12_07215"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: protein motif:HMM:TIGR00751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HET7056236.1"
                     /translation="MNATLATPSPLSIWLHAIRPKTLFASISPIIVGSAVARHEGGFH
                     LPTALVALLVALLLQILSNLANDYFDHKRGADTHRVGPLRVTQAGLVTDRQMQTAIGV
                     TIALAIAGGLYLVYRGGWPILVLGLLAIICAVAYTAGPFPLGYHGLGDLFVFIFFGLV
                     GVAGSAYVQTRDLTWFSIIAALPIACLATAIIVANNLRDIETDRAAGKHTLATRLGRP
                     GTVREYQGLFAVAYLIPLLLAIFGDVPWAWLVIPVALPIALPLVTAVQTATGKGLIPV
                     LVGTSRLTFIFGALFAIGILL"
     gene            1753..>2486
                     /locus_tag="VFI12_07220"
     CDS             1753..>2486
                     /locus_tag="VFI12_07220"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7056237.1"
                     /translation="MSQHPASGAIKPDSAVAEMFGRIVRRYDLMNRLMTGGMDARWRR
                     KAVTAALGAGNERALDVATGTGDLAIALANGGFREVVGVDFAPEMIAAAEDKRHGRSD
                     ITFQVADAMALPFPDNHFDAVTVSFGLRNMKDYQAAITEMTRVVKPGGRWICLELTPY
                     RTPVLGNLFNWYFTRVVPRVGGWLSGDAEAYHYLPTSVASFPDAKTLLDLMGIAGLAA
                     VEYELLGMGTVALHVGIKPVEQTGRRA"
BASE COUNT          400 a          905 c          756 g          425 t
ORIGIN      
        1 cgtgttctat aattgacaat ctgacacgcg actccccgct tgagaggacg agagatgaag
       61 aacaaggacg tgatgctggg atcgctgcgg ttgaacctca atgcgacgcg cgccggcgtc
      121 tccatcagct actacgatca ggatttccgt caccaccagg gcatgatgaa ggtcaagatc
      181 ggctacctga cgccggatga gctgcgctcg ggcgaggtcg acaccggaat cgacatcacg
      241 ccgatcctgc ccgtcgccgc ggagctcttc accgccgccg gcggcaccct cgacgaagac
      301 gccgcctgaa gtctcccctc tcccgctgaa cgaaggcggg agagggggtc gggggtgagg
      361 gttaccgcgg gtcgtggcgg tgcgggataa tcgcggcgaa tacctctctg gtctcgtcga
      421 tcagctcctc gatgccccag cggagcggca acccggccag cgctccgatc gtgccaaaaa
      481 ggagcggcat gccatacgag tcatagaagc gcaggccggt gaagatcgcg caggcgaccg
      541 caacaaccgg gatcgcccac cgccggctta ccaccgccga catgaccatc gcgagcaacc
      601 aggtcggttc catccgcacc tcctgccctc accatatcac ccgccctaac gggcaaagcc
      661 accgcccgta ggggcagatg gcacctgccc gcgctcatcc ccaccggacc ttcccgtgcc
      721 acgatcgggg acgggttggt agttgcgaac gggccagtcg tggcgcggtg ggcagatgcc
      781 atttgcccct acattccctc aaccctcaac ccttaacacc gactgctcgc ctcctcgcct
      841 gctcgcctcc atacttcgca ccatgaacgc aacgctcgct acgccatcgc cgctttcgat
      901 ctggctgcat gcgatccggc cgaagacgct ctttgcgtcg atctcgccga tcatcgtcgg
      961 ctcggccgtg gcccggcacg agggcggctt ccatctgccg accgcgctcg tcgctctgct
     1021 ggtcgcgctc ctgctccaga tcctctcgaa cctcgccaat gactacttcg atcacaagcg
     1081 cggggccgat acgcaccggg tcggaccgct ccgggtgacg caggccggcc tcgtgaccga
     1141 ccggcagatg cagaccgcga tcggcgtcac gatcgccctc gccatcgccg gcggcctcta
     1201 cctcgtctac cgcggcggct ggccgatcct cgtgctcggg ttgctcgcga tcatctgcgc
     1261 cgtcgcctac acggctgggc cattcccgct cggctaccac ggcctcggcg acctcttcgt
     1321 cttcatcttc ttcgggctgg tcggcgtggc cggctcggcc tatgtccaga cacgcgacct
     1381 gacgtggttc tcgatcatcg ccgccctgcc gatcgcctgc ctcgcaaccg cgatcatcgt
     1441 cgccaacaac ctgcgcgaca ttgagacgga ccgcgccgcc ggcaagcaca cgctcgccac
     1501 ccgcctcggc cgccccggca cggtccgcga gtaccagggg ctgtttgcgg tcgcctacct
     1561 gatcccgctc ctgctcgcca tcttcggcga cgtgccatgg gcgtggctgg tgatcccggt
     1621 cgccctgccg atcgccctgc cgctcgtgac cgccgtccag accgccactg gcaagggact
     1681 gatcccggtt ctggtcggca cgtcgcggct gacgttcatc ttcggcgccc tcttcgcgat
     1741 aggaatcctc ctgtgagcca gcaccccgcc tccggcgcga tcaaaccgga ctccgccgtg
     1801 gccgagatgt tcggtcggat cgtccgccgc tacgacctga tgaaccggct gatgaccggc
     1861 ggcatggatg ctcgctggcg gaggaaggca gtcacggctg ccctcggcgc cggcaacgag
     1921 cgggcgctcg acgttgcgac cggcaccggc gacctggcga tcgcgctggc caatggcggc
     1981 ttccgggagg tcgtcggtgt cgatttcgcc ccggagatga tcgccgccgc cgaggacaag
     2041 cggcacggcc gctcggacat cacgttccag gtcgccgatg cgatggccct gccctttccg
     2101 gacaatcact tcgacgcggt cacggtctcg ttcggcctgc gcaacatgaa ggactaccag
     2161 gcggcgatca ccgagatgac tcgggtcgtc aaaccgggcg gccgctggat ttgcctggag
     2221 ttgaccccgt accggacgcc ggtgctcggc aacctcttca actggtactt cacccgagtc
     2281 gtgccgcgcg tcggcggctg gctaagcggt gacgccgagg cctaccacta cctgccgacc
     2341 tccgtcgcct ccttccccga cgccaaaacc ctgctcgacc tgatgggcat cgccggcctc
     2401 gccgccgtcg agtacgagct cctcggcatg ggcacggtgg cgttgcatgt ggggatcaaa
     2461 ccagtcgagc agacgggaag gcgagc
//
LOCUS       DASSGX010000406         2145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1160272, whole genome shotgun sequence.
ACCESSION   DASSGX010000406 DASSGX010000000
VERSION     DASSGX010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1160272"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1155)
                     /locus_tag="VFI12_07225"
     CDS             complement(<1..1155)
                     /locus_tag="VFI12_07225"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7056238.1"
                     /translation="MAMRGGRRSALASASGIDNERQRQLNAEAPRIPHLRRRIAALFL
                     PYKLELGVTIALVLVSAGVTVALPLITRNIFDKALFPASGQPNMRLLGELVGLTVLLI
                     AIVAIVGIAQTWFTTRVGSRVMGDLRVQLYERLQTMEVSFFTRTKTGVIQSRLQNDVG
                     GVSNVLTNTVATVVGNSVTVVASLISMLILSWQLTIVALFLMPLMAVVQQRIGQMRAR
                     IAASTQESLSRMTSITQESLSISGILLSKAFGRQREETERYAQENQQQIQLQIREAMS
                     GQGFFAVITVFFQAVPAIVYFLAGWLMSNGNATLTAGTIVAFTTVQARLTFPLLGLMR
                     SALDLQTSGALFAHIFEYLDLRPSIVDAPDAVAVDVSQLGKVEFDHVTFRY"
     gene            complement(1220..>2145)
                     /locus_tag="VFI12_07230"
     CDS             complement(1220..>2145)
                     /locus_tag="VFI12_07230"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET7056239.1"
                     /translation="NRTMTDYGRPIEFGIFLDPAAEQSATVLDTIAFIDELGFDWIGI
                     QDHPYNRAQLDAMTLIGMGLARTERVRFVPDVATLSLRSPAMLAKQAVTLDLLGNGRF
                     ELGLGSGAFWDAIVAMGGPRRSPGEAYASLDEAIDILRAAFDGARSVDHQGEIYQLRG
                     WRPGPAPAHRIGIWLGVYGPRALRLAGRKADGWVPSLAYVPPAAAREAMQTIDSAAVS
                     AGRNPTDIRRAYNIWGGFIDAPPEPIDETSGEFAGSPAAWAAFLTHLATSIGFDTFLF
                     GVPPDSGLLRRIAGEILPRVREQVELARGRS"
BASE COUNT          427 a          693 c          692 g          333 t
ORIGIN      
        1 ataccgaaac gtgacatggt cgaattcgac cttgcccagc tgcgacacat cgaccgcgac
       61 ggcatcgggc gcgtcgacga tggacggccg caaatcgagg tattcgaaga tgtgggcaaa
      121 gagcgcgccc gatgtctgca aatccagcgc cgagcgcatc aaccccaaga gcggaaaggt
      181 gagccgcgcc tgcacggtgg tgaaggccac aatggtgccg gccgtaagcg tcgcgttgcc
      241 gttcgacatc agccagcccg ccaggaaata gacgatggcc ggcaccgcct ggaagaagac
      301 ggtaatgacc gcgaagaacc cctgaccgct catcgcctcg cgaatctgca gctggatttg
      361 ctgctgattc tcctgcgcat aacgctcggt ctcctcgcgc tgccgcccga acgctttcga
      421 aagcaggatg ccggagatgc tgagcgactc ttgcgtgatc gaggtcatgc gggagagcga
      481 ttcctgcgtg ctggcggcaa tgcgggcgcg catctgaccg atccgctgct gcaccacggc
      541 catcagcggc atgaggaaga gcgccacgat ggtcagctgc cagctcagga tcagcatgga
      601 gatcagcgag gccaccaccg tgaccgagtt gccgaccacg gttgccaccg tattggtcag
      661 cacattcgag actccgccga catcgttttg caagcgcgac tgaataacgc cggtcttggt
      721 gcgggtaaag aaggaaacct ccatggtttg cagccgctcg tagagctgca cgcgcaaatc
      781 ccccataacc cggctgccga cccgggtggt aaaccaggtc tgggcgatcc cgacgatggc
      841 gacgatcgcg atcagcagca cagtcaagcc gaccaactcg ccgagcagcc gcatattcgg
      901 ctgcccgcta gccggaaaga gcgccttgtc gaagatattg cgggtaatca gcggcaacgc
      961 gaccgtaacc ccggcgctca ccagcaccag agcgatggtg acgccaagct cgagcttgta
     1021 aggaagaaaa agagcggcga tccggcgccg caaatgggga atgcggggag cttccgcatt
     1081 cagctggcgc tggcgctcgt tgtcgatacc gctcgcactg gctaaggctg accgtcgccc
     1141 accccgcatg gccatgagac acctcccctc ccgctactct gcgctcaggg ggtcgcggcg
     1201 cgactggcag cacctcatct cacgaacggc cgcgcgcgag ctcgacctgc tcccgaaccc
     1261 gcggaaggat ctcgcccgcg atcctgcgca gcagccccga atctggcggc acaccgaaga
     1321 ggaacgtatc gaacccgatc gacgtggcga ggtgggtgag aaacgctgcc caggcggcag
     1381 gcgatcctgc gaactcgccg cttgtttcat cgatcggctc tggcggtgcg tctatgaagc
     1441 ctccccagat attgtaggcg cggcggatat cggtcggatt gcggccggcg gagacggcgg
     1501 cgctatcgat cgtttgcatc gcctcccggg cagccgcggg cgggacgtac gccagggacg
     1561 gcacccaccc gtcggccttt cgaccggcca acctgagcgc acgtggcccg tagacgccca
     1621 gccagatgcc gatccgatgc gcgggcgcgg gacctggccg ccatccccgg agttggtaga
     1681 tctcgccctg atgatcgacc gagcgagcgc catcgaacgc ggcgcgcagg atatcgatcg
     1741 cctcatccag ggaggcgtac gcttcgccgg gcgaccggcg cgggccaccc atggcgacga
     1801 tcgcatccca gaacgcgccg gagccgagtc ccagctcgaa ccggccgttg ccgagcagat
     1861 cgagggtgac cgcctgcttc gccagcatgg ccggcgaccg caatgaaagc gtggcgacat
     1921 cgggaacgaa ccggacgcgc tcggtgcggg ccagacccat gccgatcaag gtcatggcgt
     1981 cgagctgggc tcggttgtac ggatggtcct ggatgccgat ccagtcgaaa ccaagttcgt
     2041 cgatgaaggc gatcgtgtcg agcacggtcg ccgactgttc ggccgcggga tcgagaaaga
     2101 tgccgaattc gatcggccgg ccgtaatcgg tcattgtgcg gttcc
//
LOCUS       DASSGX010000407         3389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_287996, whole genome shotgun sequence.
ACCESSION   DASSGX010000407 DASSGX010000000
VERSION     DASSGX010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287996"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VFI12_07235"
     CDS             complement(<1..204)
                     /locus_tag="VFI12_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056240.1"
                     /translation="MIQRVISTIVGAVVTFLLLLLFHNGRIISNETTGYLVAVIIGAL
                     ITLFWPLIWGTYMGRRARDTQQAT"
     gene            complement(330..2015)
                     /gene="nhaA"
                     /locus_tag="VFI12_07240"
     CDS             complement(330..2015)
                     /gene="nhaA"
                     /locus_tag="VFI12_07240"
                     /inference="COORDINATES: protein motif:HMM:NF018648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HET7056241.1"
                     /translation="MSDREATQENERGVTVSVSLRIEPGRESEFDDFLAGITDAAEEF
                     PGFLSVQAYRPPRGRGRHRVLLRFDSNRSRERWRESPERRIWEDRADDISEGRPRVAD
                     ITGTSQEQPLALALGPLQEFVRTSVSGIGLLLLGAAVALIMANSHWVGTYERFWNTQV
                     TIGSVDIGITTSLRHWVNDALMALFFFILGLEIKREVLVGELREVRQAALPIAAAIGG
                     GVVPASIYALINIRGDALHGWGIPIGTDTAFALGILSLLGSRVPTVLVVFLTASSIVD
                     DIIAVAVIAIFYTDNLNVTAMIVAVGLLALLMLANRAGFHRWYVYGILGVGVWLAVFE
                     SGVHGTIAGVLVAMVVPARSWINAGDFVRVGRRLIDDFEASCSQGENILSNQQQQIAA
                     QRLDRLVEGVETPMSLMEHGLAPWVSYLVLPIFAFANAGIPLRTGLGDALGSTVTWGV
                     ILGLLIGKPIGITLFSWLSIRSGIAQSPPSTTIRDIFGVAGLAGIGFTMSLFITELAF
                     GTDPAATDARIGVIIGSIVSGSIGFFALSRTLARNGAKAAAASQHLPESGAAP"
     gene            2166..>3389
                     /locus_tag="VFI12_07245"
     CDS             2166..>3389
                     /locus_tag="VFI12_07245"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF009395.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET7056242.1"
                     /translation="MLVVMESGSSNGAAAAVRERARDAGLETILYADTEPTMIHVTGD
                     NADELAPDLAVLPGVARVMAASTSDAPVTSNLRIAGIRPLVPPAILMEQLPLPVEGAR
                     LVHQSRQEMRRILAGRDDRLIVVVGPCSVHDSEAALEYARRLRTVADELADDLRIVMR
                     VYFEKPRTTVGWKGLINDPNLDGSFAVNEGLYLARQLLLDVIAEGLPAGTEFLDPITP
                     QFIADAVSWGAIGARTTESQVHRNLTSGLSMPVGFKNGTGGGIQMAVDAMQAASYPHQ
                     FMSVTEQGLAAIVETRGNRDTHIILRGGKSGANYDAEHVQQTLGLLRTCGMPERVMID
                     ASHGNSEKDYRRQPIVIGSVAEQVAAGERGIMGVMLESFLVDGRQDLTDPAHLVPGQS
                     ITDACMGWEMTLPVLQ"
BASE COUNT          616 a         1080 c         1162 g          531 t
ORIGIN      
        1 agtcgcctgc tgtgtgtcgc gggcccggcg gcccatatag gtgccccaga tcagcggcca
       61 gaagagggtg atcagcgcgc cgatgatgac cgcaaccagg tagccggtgg tctcgttcga
      121 gatgatccgg ccgttgtgga agagcaagag caagaggaag gtaacgaccg cgccgacgat
      181 ggtgctgatc acgcgctgaa tcatgctccc ctccgggact cggtgagttc gacgatctcc
      241 catgcggcga ttcgtgaccg ccacccgacg ttactgttca caatagccga tcaggtagag
      301 agtagagggt tacgagaata accccgccat taaggggcag cgccgctttc gggaaggtgc
      361 tgagacgcgg ctgcggcttt ggccccgttg cgcgcgagcg tgcgggagag ggcgaagaac
      421 ccgatcgagc cggaaacgat cgagccgatg atcacgccga tccgggcgtc ggtggcggcc
      481 gggtcggtcc cgaaggccag ttcggtgatg aagagcgaca tcgtgaagcc gatcccggcc
      541 aggccggcca cgccgaagat gtcgcggatc gtggtgctgg gcggcgattg cgcgatcccg
      601 ctgcggatgg agagccacga gaagagcgtg atgccgattg gcttgccgat cagcaggcca
      661 aggatgacgc cccaggtaac ggtgctgccg agcgcatcgc ccaatccggt ccggagcggg
      721 atgccggcgt tggcgaacgc gaagatcggc aggacgaggt acgagaccca tggcgccagc
      781 ccatgctcca tcaggctcat cggtgtctcg accccctcga ccagccgatc gagccgctgc
      841 gcggcgatct gctgctgctg attgctgagg atgttctccc cctgggagca ggaggcttcg
      901 aagtcgtcga tcagccgccg gccgacgcgg acgaagtcgc ccgcgttgat ccacgagcgg
      961 gccggtacca ccatcgcgac cagcacgccg gcgatcgtgc catgcacgcc ggactcgaac
     1021 acagccagcc agacgccgac tccgagaatc ccgtaaacgt accagcggtg gaatccggcc
     1081 cggttcgcca gcatgagcaa cgccagcaag ccgaccgcaa cgatcatcgc cgtcacattg
     1141 agattgtcgg tgtagaagat ggcgatcacc gcgaccgcaa tgatgtcgtc gacgatcgag
     1201 gaggcggtca ggaagacgac cagcaccgtc ggcacgcggc tgccgaggag gctcaggatg
     1261 ccgagcgcaa aggccgtgtc cgtgccgatc ggaatgcccc agccgtggag cgcgtcgccc
     1321 cggatgttga tcagcgcgta gatcgacgcc gggaccacgc cgccgccgat cgccgcggcg
     1381 atcgggagcg ccgcctgccg gacctcgcgc agctcgccga ccagcacctc gcgcttgatt
     1441 tcgaggccga ggatgaagaa gaagagcgcc atcaacgcat cattcaccca gtgccggagc
     1501 gaggtcgtga tgccgatatc gaccgagccg atcgtgacct gggtgttcca gaaccgctcg
     1561 taggtcccga cccagtgcga attggccatg atcagggcga cggccgcgcc gagcagcagg
     1621 agcccaatac cggagacgct ggttcgcacg aattcctgga gggggccgag ggcgagcgcg
     1681 agcggctgct cctgcgatgt cccggtgatg tcggcgacgc ggggtctgcc ctcggaaatg
     1741 tcgtcggcgc gatcttccca gatccggcgc tccggcgatt ctcgccagcg ctcgcggctt
     1801 cgattgctgt cgaatcggag caggacccgg tggcgaccgc ggccgcgcgg cgggcgatag
     1861 gcctggactg agaggaaccc gggaaattcc tcggcggcgt cggtgatgcc agccaggaaa
     1921 tcgtcgaatt ctgactcgcg gccgggctcg atccgcagcg agacactgac cgtcacacca
     1981 cgctcgttct cctgcgtcgc ttcccggtca gacatcgcgc gctcctccgt caacggtggg
     2041 ccagttggcg gtcggccgtt ttttactgta cccgatgagc ggttctcgcc ccgtgcatga
     2101 cgaaatggcc gagacggacg aaaataggcg gattgtcgaa cttttggctc gagaggaacc
     2161 ccggcatgct ggtcgtcatg gaatctggat catcgaatgg agcggctgcg gccgttcgtg
     2221 agcgggcgcg ggacgccggg cttgagacca tcctttatgc cgataccgag ccgacgatga
     2281 tccacgtcac cggagacaac gctgacgagc tggcgccgga tctcgcggtc ttgcccgggg
     2341 tcgcgcgggt gatggccgcc tcgacctccg acgcgccggt gacgagcaat ttgcgcatcg
     2401 ccgggattcg tcccctcgtg ccgccggcga tcctgatgga gcagctcccg ctcccggtcg
     2461 agggtgcgcg gctggtccat cagagccggc aggagatgcg ccggatcctc gcggggcggg
     2521 atgaccgctt gatcgttgtc gtcggtccct gctccgttca cgacagcgag gccgcgctcg
     2581 agtacgcccg ccgcctccgg accgtagccg acgagctggc cgatgatctg cgaatcgtga
     2641 tgcgcgttta tttcgagaag ccacgcacga ccgtcggctg gaaggggctg atcaacgacc
     2701 cgaacctcga cggcagtttt gcggtcaatg agggcctcta cctcgcgcgg caattgctgc
     2761 tggacgtgat cgccgagggg ttgccggccg ggaccgagtt cctcgacccg atcacgccgc
     2821 agttcatcgc cgacgcggtg agctgggggg cgatcggcgc gcggacgacc gagagccagg
     2881 tccatcgcaa cctgacctcg ggcctgtcga tgccggtcgg tttcaagaac ggaaccggcg
     2941 gcgggatcca gatggcggtc gacgcgatgc aggccgcttc gtacccgcac cagttcatga
     3001 gcgtcaccga acagggattg gcggcgatcg tcgagacgcg gggcaaccgg gacacgcaca
     3061 tcatcttgcg cggcggcaag tccggcgcga actacgacgc cgagcacgtc caacagacac
     3121 tcggcctgct gcgcacatgc gggatgccgg agcgggtgat gatcgatgcc agccacggca
     3181 acagcgagaa ggattaccgc cgccagccga ttgtcatcgg ttcggtggcc gagcaggtcg
     3241 cggccggcga acgggggatc atgggcgtca tgctcgagag cttcctggtc gacggccgcc
     3301 aggacctgac cgacccggcg cacctcgtcc ccggccagag catcaccgac gcctgcatgg
     3361 gttgggaaat gaccctcccc gttctccag
//
LOCUS       DASSGX010000408         3011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_178775, whole genome shotgun sequence.
ACCESSION   DASSGX010000408 DASSGX010000000
VERSION     DASSGX010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178775"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            377..517
                     /locus_tag="VFI12_07250"
     CDS             377..517
                     /locus_tag="VFI12_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056243.1"
                     /translation="MGTRRLTRWVRVGLVAIALAGVTAIPMFESASAAVYSGRDNWCV
                     RC"
     gene            651..>3011
                     /locus_tag="VFI12_07255"
     CDS             651..>3011
                     /locus_tag="VFI12_07255"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET7056244.1"
                     /translation="MPDDRLVHEQESQAAAYDRPMGQGDPTSLEYPGVRFATKLTVIV
                     VSTVAVALLTGFLLDRVPFTSDQGRILAILFLFSVGSTWVPLQFAYKQEMTFEVAVVV
                     LTALLLPPDLAITVGVLGYMAGYGLRYGGRFAIDAPYNASVFALSISAAEAAFHILGW
                     NPSAERSARPEFLAAGVLAALIWFIMTRSLVSLVVMLESRQPFRQILRDSTLGMQSAE
                     QAMLGAMVALGILGAIVAQAHSAALLLLLVPAFALWLALRQNVETRHRIEASLATAQR
                     VAGIGSLDWDLKQGDIRWSDILFRILGYEPRAVQPSVDAYVSRVHPADRARVVAAFQA
                     AADGRPVGIEHRVALESGAERAFDLKFNSVTGRRGTIKRIVGTVHDITDRKQLEERLH
                     FQAYHDPLTTLPNRAMFLQRLGQAHSRQQPNRSIALLFLDLDRFKLINDTLGHDAGDQ
                     LLKIVATRLQNCVRPHDVVARLGGDEFTILLDHVHDDQEAIAVAERIISEINQPMTLL
                     GTRDVVVSTSLGIVRPGPEHTSGADLLRDADTALYRAKELGRNRYTIFDALMGAETRE
                     RLALEEDLRTAIQRGQMSLVYQPRIDLVTGRVVLVEALVRWTHPTRGEIPPTRFIPIA
                     EETGQIDAIGRWIIRTAVREAATWSRVLNPSPTLSVNITSKQLHDPDLAGGLVTTMME
                     AGLAPGRLRLEVPESVVMKNVDSAITALGALQQVGVRVAIDDFGTGHSSLTSLRRFPV
                     DTLQLDRQFVTEIGSNREATTVAQAVIGLAHGLGLRVVAAGVERPGQ"
BASE COUNT          485 a         1030 c          961 g          535 t
ORIGIN      
        1 acgccatccg ggcgggcgcc caaatcgggc gcccgcccgg ccccacaccg cgctacgccg
       61 gtgcatgcga acgggccggt cgtaagcggc gggcagatgc catctgcccc tacaatcgtc
      121 gcgcctccgc gcctccgcgc ctccaccccc aacccgactg aacggacgga ccggtacgct
      181 ggtcgtagcc cgatcgggtg gtcccagtac gtatgtctcg ccatctttct cagcctgatc
      241 ggccatagcc cgggacaatc cgccccaata ccctcgaagt agccgcaatt tgcgcaggtt
      301 ttggcgcgtg gtgcggctgc tgctcagttc gcggtcatca caaccgcgga tggcgtcgca
      361 ctcgggggat caggtaatgg gcacacgcag gttgactcgg tgggttcgcg ttgggctggt
      421 ggcgatcgcg ctggcgggag tgaccgccat accgatgttc gagagcgcct cggcggcggt
      481 ctacagcggc cgcgacaact ggtgcgtccg ctgctaaatc cgaagctggc gcggatgggc
      541 accggctgag aaacaagtca taggcaattc ggcctccgag agactatgat gaccgcgtca
      601 tcagcctcgg aggccgcttc atttactgta ccccgcatcg ggaaagccag atgccggacg
      661 atcgacttgt ccacgagcaa gagagtcagg ccgccgccta cgatcgcccc atgggacagg
      721 gagatccgac aagcctcgag tatcctggcg tgcgattcgc cacgaagctg acggtcattg
      781 tcgtttcaac cgtggcagtg gccttgctca caggattcct ccttgacagg gttccgttca
      841 ctagcgatca gggcaggatt ctggcgatcc tgttcttgtt ttccgttggt tccacctggg
      901 ttccgctcca attcgcgtac aagcaggaga tgaccttcga agtcgcggtt gttgtcctga
      961 ctgccttgct cctgcctccc gatttggcga tcacagtcgg cgtgcttggg tacatggcgg
     1021 gatatgggct tcggtatggc ggccgatttg cgattgacgc cccctacaac gcaagtgttt
     1081 tcgccctgag catctccgcc gctgaagccg cattccacat cctcggttgg aatccgtccg
     1141 cagagcgatc ggcccggccg gagttcctgg ctgctggagt gctggcggcg ctgatctggt
     1201 tcatcatgac ccgctcgctg gtgtcgcttg tcgtcatgtt ggaaagccgg cagccgtttc
     1261 gccagatcct gcgggacagc acgctcggca tgcagtcggc cgagcaggcg atgctcggtg
     1321 cgatggtcgc cctcggcatc ctcggcgcga tcgtcgcgca ggcccattcc gctgccctgc
     1381 tcctgctgct cgtgccggcc tttgccctct ggctcgcgct tcgccagaac gtcgagacgc
     1441 ggcaccggat cgaggccagc cttgccactg cccagcgcgt ggccgggatc ggctcgctcg
     1501 actgggacct caagcagggc gatatccgct ggtccgacat cctcttccgc atcctcggct
     1561 acgagccgcg cgcggtgcag ccgtcggtcg atgcctatgt cagccgggtc catccagccg
     1621 atcgagcgcg cgttgttgca gcatttcagg cggcggccga cggccggccg gtcggcattg
     1681 agcaccgggt cgcgctggaa tccggcgccg agcgagcctt tgacctcaag ttcaattccg
     1741 tcacagggcg gcgcggcacg atcaagcgga tcgtcggcac cgttcacgac atcaccgacc
     1801 gcaagcagct cgaagagcgg cttcatttcc aggcgtacca cgatccgttg accaccctgc
     1861 cgaaccgggc catgttcctg cagcggcttg ggcaggctca cagccggcag cagccgaatc
     1921 ggtcaattgc gctcctcttc ctcgatctcg accgcttcaa actgatcaac gacacgctcg
     1981 gccacgacgc cggcgatcaa ctcctgaaga tcgtcgccac gcggctgcag aactgcgtgc
     2041 ggccgcacga cgtcgtcgcc cggctgggcg gagacgagtt cacgatcctg ctcgaccacg
     2101 tccacgacga ccaggaggca atcgccgtcg ccgagcggat catcagcgag atcaaccagc
     2161 cgatgactct gctcggcacg cgcgacgtcg tcgtctcgac cagcctcggc atcgtccggc
     2221 ccggcccgga gcacacctcc ggcgccgatc tgctgcggga tgctgatacc gcgctctacc
     2281 gggccaagga gctcggccgg aaccggtaca ccatcttcga tgccttgatg ggcgccgaga
     2341 cccgggagcg gctggcgctc gaggaggatc tgcgcaccgc gatccagcgc gggcagatgt
     2401 ccctcgtcta ccagccgcgg atcgacctcg tgaccggccg cgtcgtgctg gtcgaggcgc
     2461 tcgtgcggtg gacccatccg acccggggcg agatcccgcc gacccgcttc attccgatcg
     2521 cggaggagac gggccagatc gacgcgatcg gccgctggat cattcgcacg gcggtgcggg
     2581 aggccgcgac ctggtcccgc gtgctcaacc cctcgccgac cctgagcgtc aatatcacca
     2641 gcaagcagct ccacgacccc gatcttgcgg gtggcctggt gacgacgatg atggaagcgg
     2701 gcctcgcgcc cggacggctc cggctggagg tgccggagag cgtcgtcatg aagaacgtgg
     2761 atagcgcgat cactgccctc ggcgcgcttc agcaggtcgg cgtcagggtc gcgatcgacg
     2821 atttcggcac cggccattcc agcctcacct cgctccgtcg cttcccggtc gacacgctcc
     2881 agctcgatcg ccagttcgtg accgagatcg gcagcaaccg ggaggcgacg acagtcgcgc
     2941 aggcggtgat cggcctcgcc cacggcctcg ggctgcgggt cgtcgcggcc ggcgtcgagc
     3001 gaccggggca g
//
LOCUS       DASSGX010000409         4492 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_615441, whole genome shotgun sequence.
ACCESSION   DASSGX010000409 DASSGX010000000
VERSION     DASSGX010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4492
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615441"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..999
                     /locus_tag="VFI12_07260"
     CDS             43..999
                     /locus_tag="VFI12_07260"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET7056245.1"
                     /translation="MSAPMIIAVAAFVAVCATVVLVSMLTQRRSDAVLRRLDDHAVAV
                     AKVAAKRRERINVLKDRADGSLLGRFRPGETATVELERAGSTMSVARYLLLRLALAVI
                     AFVVVAVAFGYPFLGLPVAVVAALAPRVVLMRQARRRLLRFEAQLAEAIDLLVGALRA
                     GHGFLQGLESVAKQIEDPTRHEINRVLEEVNVGIGPVEALEGVRERIPSYDLALLVSA
                     ISVQRQTGGNLAEVLENLASTVRERRRVRGEVHALTTGPRVSSYVLGAIPALLFLYFI
                     AISADYRDIMLGTMYGHILLGLAAVLSTLGYVFSRKVSKVEY"
     gene            1052..1966
                     /locus_tag="VFI12_07265"
     CDS             1052..1966
                     /locus_tag="VFI12_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET7056246.1"
                     /translation="MTFLLSSLVFVAVFGAVLVVSRRSQRAVFQINRHQIMPAGAGMV
                     AAPRVRTNILGNTGEGGVAAQFATRITRPEVRTKSGRLLREAGSPMPLATYLLMRVVC
                     TFAIMPLFVLFIWHGFGPSPIGIVAMVVGGFTIPQLPLLRIKRKARFRARAIDAAMPD
                     ALDLLVVCAEGGLSLDGAVQQVSQRTSGVLAAEFRRLLQEIGSGMVRREAFLSLGERS
                     QSESLKIFCATIIQADKMGMSIATTLRTLADTMRTRRRQAAETQARKAPIKMMPFLVI
                     FMIPSLFIVILGPAILSIIDFMRDVSKM"
     gene            complement(2020..2565)
                     /locus_tag="VFI12_07270"
     CDS             complement(2020..2565)
                     /locus_tag="VFI12_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056247.1"
                     /translation="MSLKDRYNRGDGRIQSFDKYLTDIGDDLGEFWWRITGVHRSVLT
                     QGLYVVSAWAGVQHFMVTRDATMLLITCVALLSFMGIGQSKGGLVEQLQAEATGLPKN
                     SIAFMRLLIIGIGVFSLISAACTLAASFTGELITTRAVAEPMLVGLALTALQLSDYIR
                     RTNPATPGKGGRGKLHRIDVR"
     gene            2931..3218
                     /locus_tag="VFI12_07275"
     CDS             2931..3218
                     /locus_tag="VFI12_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056248.1"
                     /translation="MRIDADMAAADLFGDDTKCHVELIGPTVGERITPPAPVERASAD
                     LPTGGRQQIRVAQDGVRREVARRVRHGRELVRDAALVSAYAPQPEVWPAGE"
     gene            3358..4314
                     /locus_tag="VFI12_07280"
     CDS             3358..4314
                     /locus_tag="VFI12_07280"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HET7056249.1"
                     /translation="MSRSNGSSREPAPWRDRVSQALPFLETFVVIASIAVIAGCLYIF
                     WVLVTDPERGEVWTSGLHPAHFYRDLDAELLDDYANTFVVGHNSGDRIDSALQAIYYG
                     ADVIEVDVVAVNGRLYAGHDAPPTWIGDNLFRGPPLDRIWVAASGAQAVKLDLKESSP
                     AFVALVMDFLAQHRGQRQVIVVSGDPAVLRRIGEQEPSVFRLLSIGSEPRLESIFENP
                     ELVDGLDGVSIRESLITGERMEWLADHHLLTFAWTVNDLERVNELVLLGVDAITTDNL
                     AIMRLLGGRSGREKRLERKPAAHPSPAAATATIGLLDRWRIL"
     gene            complement(4271..>4492)
                     /locus_tag="VFI12_07285"
     CDS             complement(4271..>4492)
                     /locus_tag="VFI12_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056250.1"
                     /translation="STFRWRRRWTARLVLAAVVLAVLTYVAAANFVVVELRLVGWVGD
                     VRLSWIVLGSAGLGLVTGFAIGRANRWLP"
BASE COUNT          778 a         1358 c         1506 g          850 t
ORIGIN      
        1 ggggcggagc gaggggatga gcaaaaaaag gggagagctg ccatgtccgc gccgatgatc
       61 attgccgtcg cggccttcgt tgccgtatgc gcgaccgtgg tgctggtctc gatgcttacc
      121 cagcgccggt cggacgcggt gctccggcga ctggacgatc atgccgtagc ggtagccaag
      181 gtcgcagcca agcggcgcga gcgaatcaac gtcctgaaag atcgcgccga cggctccttg
      241 ctcggccggt tccgcccggg cgaaacggca acggttgagt tggagcgggc cggatcgacg
      301 atgagcgttg cccgatattt actgctccgg ctcgcgctgg ccgtgatcgc cttcgtcgtc
      361 gttgcggtag cgttcgggta cccgttcctg ggactgccgg tggcggtcgt cgccgctttg
      421 gcgccgcgcg tcgtattgat gcggcaggcc aggcggcgat tgctgagatt cgaggcgcag
      481 ctcgctgagg cgatcgattt gctggtcggc gcacttcggg ctgggcatgg cttcctgcag
      541 gggctggagt cggtcgccaa gcagatcgag gacccgaccc ggcatgagat caaccgcgtg
      601 ctggaggaag ttaatgtcgg catcggcccg gtcgaggcgc tcgaaggcgt gcgggagcgg
      661 atcccgagct acgacctggc gctgctcgtc tccgcgattt ccgtgcagcg gcagaccggc
      721 ggcaatctgg ccgaggttct cgagaacctg gcctcgaccg tccgggagcg ccgcagggtc
      781 cggggcgaag tccacgcgct gaccacgggg ccgcgcgtgt cgagctatgt gctgggcgcg
      841 attccggccc tgctgttcct ctacttcatc gcgattagcg ccgattaccg ggacatcatg
      901 ctcggcacga tgtatggcca tatcctgctc ggcctggcgg cggtgctctc cacgctcggg
      961 tatgtcttct cccgcaaggt ttcaaaggtg gagtactgat gttggctgcg aacgggacca
     1021 gccagcctga ccaaggagag gagcgttcgg cgtgaccttc ctgctttcga gcctcgtttt
     1081 cgtggctgtc ttcggcgcgg tcctcgtggt cagccgccga tcgcagcgcg ccgtgttcca
     1141 gatcaatcgt caccagatca tgccggccgg cgcggggatg gtggcggcgc cccgtgtccg
     1201 gacgaatatc cttggcaaca ccggggaggg aggtgtcgca gcgcaattcg cgacccggat
     1261 cacgcgacca gaagtgcgaa ccaagtccgg ccggctcttg cgcgaggccg gcagccccat
     1321 gccgcttgcg acgtacctgc tgatgcgggt ggtttgcaca ttcgcgatca tgcccctctt
     1381 cgtgctcttc atctggcacg ggttcgggcc gtcgccgatt gggatcgtag ccatggtcgt
     1441 cggcggattc acgattccgc aattgccgtt gcttcggatc aagcgcaagg cccgctttcg
     1501 agcccgggcg atcgatgccg cgatgccgga cgcgctggat ttgctcgtcg tctgcgcgga
     1561 aggcggattg agcctggacg gtgccgttca gcaggtctcg cagcggacaa gtggcgtgct
     1621 ggcggccgag ttccggcggt tgttgcagga gatcggcagc ggtatggtcc ggcgcgaagc
     1681 cttcctgtcg ctcggcgagc gatcgcagtc cgagagcctc aagatcttct gcgcgacgat
     1741 tatccaggcg gacaagatgg gcatgagcat cgcgacgacg ctgcgcacgc tggccgatac
     1801 catgcggacc cgacggcgtc aggccgcgga gacgcaggcc aggaaggcgc cgatcaagat
     1861 gatgccgttc ctggtcatct tcatgatccc atcgctcttc atcgtcatcc tgggcccggc
     1921 gatcctctcg atcatcgact tcatgcgtga cgtgtcgaag atgtgattac ggaccgaggg
     1981 gtgatgctga tgctgacggc tggtcagaca aatacctggt taccgaacat cgatgcgatg
     2041 aagcttgcca cggccgccct tgcccggagt cgccggattg gtacggcgga tgtaatcgct
     2101 cagctggagc gctgtcagcg ccagaccaac cagcataggt tcggcaacgg cgcgggtcgt
     2161 gatcagttca ccggtgaagg acgccgcgag ggtgcatgct gcagagatca gactgaagac
     2221 ccctatgcca atgatcagca gccgcatgaa ggcgatggag tttttgggca ggccggtcgc
     2281 ctcagcctgg agctgctcaa ccaacccgcc cttcgactga ccaatgccca tgaagctcag
     2341 aagggcgacg caggtgatga ggagcatggt cgcatcacgg gtgaccatga agtgctggac
     2401 gccggcccag gccgatacga cgtacaggcc ctgcgtcagc acggaccgat ggacgccggt
     2461 gatccgccac caaaactcgc caaggtcgtc gccgatatcg gtcaggtact tgtcgaacga
     2521 ctgtatgcgg ccgtcgccgc ggttatagcg gtccttgaga ctcacacacg cgtcctttca
     2581 cccaatccgc gagcgagtcg cgggtacgtg caggcctgtg aatccgtgct cggcaggggg
     2641 acggggacag tgcccgattg gtcgatggaa ctttccgcct gaccaggtgc agtcaatacg
     2701 tacccgggct gccgaccgaa tagttacccg cagtatcaca caagagtacc attgccgaaa
     2761 gtggcacgac acacattgtc cccggttctc cgctactgac gcatgaaccc agcacaggag
     2821 aaggatcgcc gcgcagctgt gagcggtgat ctaccagccg aatacaccgg gcgaatggga
     2881 agctggacct caagttctcg aacccgaccg acctctggat gttgctccag atgcgaatcg
     2941 acgcggacat ggcggcggct gacctgtttg gcgatgacac caaatgtcat gtcgagctga
     3001 tcggaccaac ggtcggggag cggatcactc cgccggcccc ggtcgagcgt gccagcgcgg
     3061 acctgcccac tggcgggcgc cagcagatcc gggttgcgca ggacggcgtg cggagagaag
     3121 tggcccgccg cgtccgtcat ggtcgggaac tcgttcggga cgcggccctc gtcagcgcct
     3181 acgcgccgca gccggaagtt tggccggcgg gtgagtagcg cggaagtcga tctaaaccgt
     3241 gcgtactggc gcgatctcat ttccggctgg ttgcgatgcg cgtcgagtcg tgtgcggtct
     3301 tcgcgttaga gtgctgattg gtctgatcgt gtcgcatcgt ccggcgtggg tgtgctgatg
     3361 tccaggtcca atggctcctc gcgggagccg gcaccatggc gagatcgcgt ttcgcaggcg
     3421 ttgccgttcc tcgagacgtt cgtcgtaatt gcctcgattg cggtcattgc gggatgcctc
     3481 tacatctttt gggtactggt cactgatccg gagcggggtg aggtgtggac gagcggattg
     3541 cacccggctc acttttaccg cgatctggat gccgaactgc tcgatgacta cgccaacacg
     3601 tttgtcgtcg gtcacaactc gggtgaccgg atcgattccg cgttgcaagc catctattac
     3661 ggcgcggatg tgatcgaggt tgacgtcgtt gcggtgaacg ggcggctcta tgccggccac
     3721 gatgctcccc cgacctggat cggggacaac ctgtttcgcg gtccgccgct cgatcgcatc
     3781 tgggtcgccg ccagcggcgc tcaggccgtc aagctcgatc tgaaggaatc aagcccggcg
     3841 ttcgtggcgc ttgtcatgga cttcctcgcc caacatcggg gacagcggca ggtgattgta
     3901 gtttcggggg atcctgcggt cctgcgaagg atcggcgagc aagagccatc cgtgtttcga
     3961 ctgctgagca tcggctccga gccccgattg gagtcaattt tcgagaatcc ggagctggtc
     4021 gatgggctgg acggggtttc cattcgagaa tccctgataa ccggcgagcg catggagtgg
     4081 ctggccgatc atcaccttct gacgttcgcc tggactgtca acgatcttga gcgggtcaat
     4141 gagctggtgc tcctgggggt ggatgccatc acgaccgaca acctggcgat catgcggctg
     4201 ctcggtgggc ggagtggcag ggaaaagcgt ctggagcgaa aacccgccgc ccacccgtcc
     4261 cccgccgcgg ctacggcaac catcggtttg ctcgaccgat ggcgaatcct gtaacgaggc
     4321 cgagaccggc cgacccgagc acgatccacg aaaggcggac atcgccgacc cacccgacca
     4381 ggcgcaactc gacgacgacg aagtttgcgg ctgccacgta tgtcagcacc gcgagcacga
     4441 ccgctgccag cacgaggcgc gcggtccacc ggcgtcgcca ccggaatgtc ga
//
LOCUS       DASSGX010000410         2444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_397977, whole genome shotgun sequence.
ACCESSION   DASSGX010000410 DASSGX010000000
VERSION     DASSGX010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397977"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(209..952)
                     /locus_tag="VFI12_07290"
     CDS             complement(209..952)
                     /locus_tag="VFI12_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056251.1"
                     /translation="MVSREQIQINGAPIRIHGSFDREVQDESGRPTSEMEVVAIIRGR
                     LPNKQFMQLISQETVRLDYQDGQTAVTMVTRVVNHSAVASGTGEGTVYRHDITFRELS
                     ESFQRRSAERAIVAAAKAAEAPRPSPRVAVQEREQAGDISDVTTATDPSSWGDAIRQL
                     RGSMPQPKAPEEPLTMPELAGIETVLVSLRLEALIHVLESAGFVPAGAAEAQFRDLLE
                     ERFFEDASQIVGERIAQRALREMHASHQT"
     gene            1167..2336
                     /locus_tag="VFI12_07295"
     CDS             1167..2336
                     /locus_tag="VFI12_07295"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7056252.1"
                     /translation="MPTPPRLNVADPLNLESEYTPFERELRDTTRAFAQEQIAPNVQA
                     WWDAEAIPRDLIIEFGKLGIIGLMHQPGGEQHAIAYGLVNAELDAVDSGVRTLFSVQG
                     GLAMTAIYRFGSNEQRDHYLPKMRTGEILGALALTEPESGSDPGSMTTIARRDGSDWV
                     LNGYKRWNTNCLIADIAITWAMTDEGIRGFIVPTDAKGLERRAVTGKYSLRAAVATEF
                     EMHDVRLPADAVLPNVVGLKGPLACLSEARYGIIWGMLGVMRSCLETALAHSTGRVQF
                     GKPIGAYQLTQAKLSDMLLKYSQAQLLAHQIGRLKQRGELTTEQISMGKLNNVNAAIE
                     VARTARSILGGNGITSMYPVMRHMANLESILTYEGTAEVHTLIIGQGLTGLSAFR"
BASE COUNT          427 a          875 c          710 g          432 t
ORIGIN      
        1 tctcataatc agaccggaat cgtcagactt tcgatagggt ctctcgtcgt tcgtcactcg
       61 tcgttcgtct ctcgtctctc gtctctcgtc tctcgtcttt agttttccgt cttccgtttt
      121 ccgctttccg tatccatcta ccaacgacca acgaccaacg accatcaacc gtctcacgtc
      181 tcacgtctca cgtctcacgt ctcacgtctc acgtctggtg actcgcgtgc atctcccgta
      241 gggcgcgctg ggcgatccgc tcgccgacaa tctggcttgc atcttcgaag aagcgctctt
      301 ccagcaaatc ccggaactgg gcctccgccg ccccggccgg gacgaaccca gccgactcca
      361 gcacgtggat caacgcctcc agtcgcaggc tgaccaggac cgtttcgatc ccggccagct
      421 ccggcatcgt cagcggctcc tccggcgcct tcggctgcgg catgctgccc cggagctgcc
      481 tgattgcgtc gccccagctc gacggatccg tcgccgtcgt gacatccgag atatccccgg
      541 cctgctcccg ctcctggacc gcgacccggg gcgacggtcg tggcgcttca gccgccttgg
      601 ccgccgcgac aatcgcccgc tccgctgacc ggcgctggaa cgactccgac agctcccgga
      661 aggtaatgtc atgccggtag accgtgccct cgcctgtgcc gctcgccacc gccgagtggt
      721 tgacgacccg cgtgaccatg gtcaccgccg tctggccgtc ctggtaatcg agccgtacgg
      781 tctcctggct gatcagctgc atgaactgct tgtttggaag ccggccacga atgatcgcga
      841 ccacctccat ctcgctggtc ggccggccgc tctcgtcctg cacctcgcga tcgaagctgc
      901 cgtggatgcg gattggcgcc ccgttgatct gaatttgctc ccgcgaaacc acctcagccc
      961 ctcccaaggg aacgcacacg accgccgcac gacggacacc gtcgattcat cctattggct
     1021 accttggcgt ccggacagtc caccatctgt accggctgtc ccggctgttc tggtcacacg
     1081 ggccgggcat tggggaccgg aatccggcag ccgtccccta cacttttcgc atgtccttcc
     1141 gctgaatcag ggaaacaccc gtgagcatgc caacgccccc tcgcctcaac gtcgccgatc
     1201 cactgaacct cgagtccgaa tacacaccgt tcgagcgcga gttgcgggac acgacccgcg
     1261 ccttcgccca agagcagatc gcgccgaacg tgcaggcgtg gtgggatgcc gaggcgatcc
     1321 cgcgcgacct gatcatcgag ttcggcaagc tcggcatcat cggcctgatg caccagcccg
     1381 gcggcgagca acacgcgatc gcctacggcc tcgtcaacgc cgagctggac gctgtcgaca
     1441 gcggggtgcg caccctcttc tcggtccagg gcggcctcgc gatgaccgcc atctaccgct
     1501 tcggctcgaa tgagcagcgg gaccactatc tcccgaagat gcgcaccggc gagatcctcg
     1561 gcgccttagc cctgaccgag ccggaatcgg gcagcgaccc cggcagcatg accaccattg
     1621 cccggcgaga cggctccgac tgggtcctga acggctacaa gcgctggaac accaactgtc
     1681 tgattgccga tatcgccatc acctgggcca tgaccgatga gggcattcgc ggcttcatcg
     1741 tcccaaccga cgcgaagggg ttggagcgcc gcgcggtgac cggcaagtac tcgcttcggg
     1801 cggccgtggc gaccgagttc gaaatgcacg atgtccgctt gccggccgat gcggtcctgc
     1861 ccaacgtcgt gggcctcaag ggtcccctcg cctgtctgag cgaggcccgc tacgggatta
     1921 tctgggggat gctcggcgtg atgcggagct gcctcgagac ggcgctggcc cacagcaccg
     1981 ggcgcgtcca gttcggcaag ccgatcggcg cctaccagct cacccaggcc aagctgagcg
     2041 acatgctgct caaatacagc caggcccagc tcctcgcgca ccagatcggt cgtctcaagc
     2101 agcgcggcga gctaacgacc gagcagatca gcatgggcaa gctcaacaac gtcaacgccg
     2161 cgattgaggt cgcccgcacg gccaggagca tcctcggcgg caacggcatc accagcatgt
     2221 acccggtcat gcgccacatg gccaacctcg aatcgatcct cacctacgaa ggcaccgccg
     2281 aagtccacac cctcatcatc ggccagggat tgaccggcct gtcagcgttt cggtaggcga
     2341 gcagcccgga ggcgagcaga cgagcagcag ggcgcgaagg cgtggagatg taggggcaga
     2401 tggcatcgcc aatgctgtcg cattaaccgc tgcggaggag ggtt
//
LOCUS       DASSGX010000411         3212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1268006, whole genome shotgun sequence.
ACCESSION   DASSGX010000411 DASSGX010000000
VERSION     DASSGX010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268006"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            339..1559
                     /locus_tag="VFI12_07300"
     CDS             339..1559
                     /locus_tag="VFI12_07300"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7056253.1"
                     /translation="MARSPAVGGTFSALQTNPLFRRLWFASTGYSLSQWMQNIALGWL
                     AFELTDSERFVGLVAFSAGIPFVAVSIPGGALLDRFDRRSVLLISQALAAVVATCVAI
                     DVLSGNAEAWHLLLAGFLNGSLQAIITPSQQSIVPRLVEPRDLQNALGLMSAGGNMTR
                     VFGPALAGTLIAALGTGYPFLLQALAVSAAFMVIFSSDFPRMPPSTGRLGLDVISDGA
                     RIITSRPDLRELFMLSALPSLLIFPYLSFLNVYAEEVMKIGSEGLGVLLAASGIGAVI
                     GGLMVAATKTSDGMGIRLYRLTFLYCLMLVAFSIYPSWWVAIPSLCLAGLVGSYAFSG
                     NNALVQQRISDEVRGRVMGTYLLTWGLMPLGALWMGEVGQLWSIQVATFAGAAVCAAA
                     VLLLRFKSQELHAI"
     gene            complement(1556..2734)
                     /locus_tag="VFI12_07305"
     CDS             complement(1556..2734)
                     /locus_tag="VFI12_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056254.1"
                     /translation="MNSRRTDQRNSPPRERHRYPQPTLLRSLLDRRLMGLADRGGASG
                     FVGLAWGDYCRSWLMDQIGKSITVGEQIHGVVDSVMDIDAIPGLAAFASSRALQNPDF
                     VITLVTPDGPMLVAIDAKFSIETAKPRQVSAEILTALLETPGTPVRPLVPAHGVERDG
                     FFITPDYELTHLVLNGTTGILRASVRDEQVCLLDAPPDELFARPDLHALIDALAAIDS
                     NQVDWGTDLVAALYYVRCAFAGMGCRIDETKALLGRSTVRELDDPGFLSELVERGRQT
                     PSAWQLVQRWDRDAEDVRAIRIRVHQAAEIGIPNRDLRTLVERESERLGQPTPSMNRI
                     RRELTIWANQELEQRFGTVFHPLPDLGRFLIDLRRAVNELQPEVPARVREIVAGIAPA
                     "
     gene            2783..3070
                     /locus_tag="VFI12_07310"
     CDS             2783..3070
                     /locus_tag="VFI12_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056255.1"
                     /translation="MGSDTPALDTPQSPFDPFISQLMTLIATEDHLSFSELNQLCVRE
                     FHWLPGFCDVVINILRTNGLIQVFEWEPGRVHISERGRHWTHASRESVSLR"
     gene            3084..>3212
                     /locus_tag="VFI12_07315"
     CDS             3084..>3212
                     /locus_tag="VFI12_07315"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HET7056256.1"
                     /translation="MTPQTRSGAELVVRALETAGVDTVFGIPGVHTLALYDALLKSG"
BASE COUNT          542 a         1061 c          979 g          630 t
ORIGIN      
        1 attccttccc gttcacggtc atggttacag tgaccttgct cggggcaacc atggtatccg
       61 ccattccgcg cctcgctccc ttcttcatcc agcctcggtc cgcgccttcg cggcacgaga
      121 agttcgccga tgatggttga ttctcgcgcg attatatagc cggttccgca ggcgtggggc
      181 atggacttcc cgagtaatcg ctgggcggag ccgcaatcag cagatctgac ctcgaccatg
      241 tagtctttgc cgagcacgtt gatcattcgt ggagcaagcc ccgcgtggcc gtcagggtcc
      301 gacctgcaac gaaaccagct cgtcggatcc ggttcgggat ggcccgtagc ccggcagtcg
      361 gcggcacctt cagcgcgctc caaaccaacc cgctatttcg gcggctttgg ttcgcctcga
      421 ccgggtactc cctgagccag tggatgcaga acatcgcgct cggctggctc gctttcgagt
      481 tgaccgattc ggagcggttc gtcggattgg tcgcgttttc agccggcatc cccttcgtcg
      541 ccgtctcgat cccgggtggc gcgttgctcg atcgcttcga ccggcgatcc gtcctcctga
      601 tcagccaggc gttggctgcg gttgtcgcga cctgcgtggc gatcgacgtg ctctccggca
      661 atgccgaggc gtggcacctg ttgcttgccg gattcctgaa cggctcgctg caggcgatca
      721 tcacgccgtc gcagcaatcg atcgtgcccc gtctggtaga gccgcgcgat ttgcaaaacg
      781 cgctcggact gatgagcgcc ggcggaaaca tgacccgcgt cttcggcccc gccctggccg
      841 ggacgctcat cgccgccctt ggcacgggct acccgttcct gctccaggca ctcgccgtct
      901 cggccgcatt tatggtgatt ttctcctccg actttccgcg catgccgcca tcgaccggac
      961 ggcttgggct cgacgtcatc tcggatggcg cccggatcat tacctcacgc cccgatctcc
     1021 gcgagctctt catgctcagc gccttgccga gtcttctgat cttcccctac ctctcgttcc
     1081 tgaatgttta cgcggaagaa gtcatgaaga ttggctccga gggcctcggt gtgctcctcg
     1141 ccgcgtccgg catcggcgcg gtcatcggcg ggctgatggt tgccgcgacg aagaccagcg
     1201 atggcatggg aatccggctc taccgcctga ccttcctcta ttgcctgatg ttggttgcct
     1261 tttcgatcta cccgagctgg tgggtggcga ttccaagtct ctgccttgcc gggctcgtgg
     1321 gctcctacgc cttcagcggc aacaacgcac tggttcagca acgaatcagc gacgaagttc
     1381 gcggccgagt gatggggacc tatctgctga cgtggggact catgccgctc ggcgcgctct
     1441 ggatgggcga agtcggccag ctttggagca tccaggttgc cacctttgcg ggagctgccg
     1501 tctgcgccgc cgcggtgctc ctgctccggt tcaagagcca ggagctgcat gcgatctagg
     1561 cgggagcgat tccggccacg atctcgcgca ccctggccgg cacctccggc tgcaattcgt
     1621 tgacagcccg gcgcaaatcg atcaggaagc gtcccagatc cggtaacgga tggaagacgg
     1681 tcccgaatcg ctgctccaac tcctgattcg cccagatcgt caattcgcgg cggatgcgat
     1741 tcatggacgg cgtgggttga cccaaccgct ccgattcccg ctcaaccagc gtccgcagat
     1801 cccggttcgg aatgccgatt tcggccgcct ggtggaccct gatccggatc gcgcgcacgt
     1861 cctcggcatc acggtcccag cgctgcacga gctgccaggc ggacggcgtc tgccggccgc
     1921 gctccaccag ctctgacagg aaccccggat catcaagctc ccgaacagtg ctgcggccga
     1981 ggagtgcctt cgtctcgtcg atccggcacc ccataccggc gaaggcgcag cgcacgtagt
     2041 agagcgcggc aacgagatcc gtcccccaat cgacctggtt gctgtcaatg gctgccaatg
     2101 cgtcgatcag agcgtgcagg tctggccgtg cgaacaactc gtcggggggc gcgtcgagca
     2161 ggcaaacctg ttcatcccgg acggatgcgc gcaggatgcc ggtcgtccca ttcaggacga
     2221 gatgcgtcag ctcgtaatcc ggggtgatga agaagccgtc gcgctccacg ccgtgcgccg
     2281 gcacgagcgg gcgaaccgga gtgccgggag tctccaggag cgcggtcagg atctccgcgc
     2341 taacctgtcg tggcttggca gtctcgatcg agaacttggc gtcgatcgcg accagcatcg
     2401 gcccgtccgg cgtgacgaga gtaatcacga agtcgggatt ctgaagcgcg cggctggatg
     2461 cgaaggccgc caaaccggga atcgcgtcga tatccatcac cgaatcgacg acgccgtgaa
     2521 tctgctcccc gacagtgatc gatttcccga tctggtccat caaccaggac cggcagtagt
     2581 cgccccaggc cagcccgacg aacccgcttg ccccgccgcg atcggccagc cccatcaacc
     2641 gccggtcaag aagtgagcgc agcaaggtgg gctggggata gcgatgccgc tcgcgagggg
     2701 ggctattgcg ttggtcggtt cgtcggctgt tcaccggaaa atcatagccg ttcggcgccg
     2761 tcgatggtgg taccatcccg ccatgggaag cgacacgcca gcgctcgaca caccacaatc
     2821 gccgttcgat ccctttatca gccaactcat gacgctgatc gcgacagagg atcatctctc
     2881 cttttcggag cttaaccagc tctgcgtccg tgagttccac tggctgccgg ggttttgcga
     2941 cgtggtgatc aatatcctgc gcacgaacgg cctgatccag gtcttcgaat gggagcccgg
     3001 tcgcgtccac atttcggaac ggggccggca ttggacccac gcaagccggg aatcagtctc
     3061 actcagatag aaggaactac cagatgacac cacagacgcg atcgggcgcc gagttggtcg
     3121 tgcgcgcact cgaaacggcc ggagtagaca cggtcttcgg catccccggc gtccacacac
     3181 ttgcgctcta cgatgccctg ctcaaaagcg gg
//
LOCUS       DASSGX010000412         2407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1488807, whole genome shotgun sequence.
ACCESSION   DASSGX010000412 DASSGX010000000
VERSION     DASSGX010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1488807"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /locus_tag="VFI12_07320"
     CDS             <1..932
                     /locus_tag="VFI12_07320"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET7056257.1"
                     /translation="SRVNNVIARMGGSGRRLAIMAHADEICFMVKSVTDDGFLHIWPY
                     YQDTRGHPPRWVLPLNQPAIVMTSREPVEGYFATASGHVVTNREPAAKWSWNDWFIDI
                     GVSSKQEATDLGVGPGCHAIWNPPTKRLRDGRITGKAMDDRAALAIATLAGERLASRT
                     DLAYEVWLVSTIQEENGLIGAQSVADEIDLDLCINLDVGLTGDVPGVDVRDFPSKLGA
                     GPIVVYQDGSAHYSRSLSDRLVALAGHNDIPVQRAVFQQYGSDAAALLRRGVDVSLLT
                     YPTRYTHSPIETVDERDIEHCVDLIVAFATTAV"
     gene            999..>2407
                     /locus_tag="VFI12_07325"
     CDS             999..>2407
                     /locus_tag="VFI12_07325"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HET7056258.1"
                     /translation="MRAWLSNLRLRVLLLVLVAVLPAVGLLLVTVSEQRDEAVQNAEA
                     AARQLVGLAAADQGRQIESTRQLLTVLAQFPEVQSGGQACNELFADLLTQFPLYANLG
                     VIGLDGMVDCSGVPTDGPVYLGDRTYFQRVVASQVFVVGEYQTGRITEKPALNTGYPV
                     IKDNKLVGVVYAAIDLNSLAQFAAQARLPEGAVLTVLDRSGRVLARVPPEQQLVGQSL
                     VGTPVVDTILAQGTGTTEQKLDDQTYVIAFESLRSAEQGNAYLSLALPKSDITASAEK
                     RFGDNLTRLGLAVMVILIAAWVGTDLLVRRSPESYRVLVRRYYDAFSTGGLDLMEEVV
                     ADDFLDHDPMPGQPAGLLGLKQAVGEFRAAFPDGEMVVDDLVAEGDRVVARVTMSGTH
                     RGEYAGMPGTGRAVRAEGIEIFRVAHGKIAEGWSRFVLPLAMIEDAPDILANGETSAE
                     IDLDESETGRTGPIRSVLES"
BASE COUNT          417 a          781 c          789 g          420 t
ORIGIN      
        1 gatcccgcgt caacaatgtc atcgctcgca tgggcggctc gggccggcgc ctggcgatca
       61 tggcccacgc agacgagatc tgtttcatgg tcaagagcgt caccgacgac ggctttctgc
      121 acatctggcc ctattaccag gatacccggg gccatccccc gcgctgggtc ctcccgctca
      181 atcaaccggc cattgttatg acaagcagag agccggtcga gggctacttc gccactgcca
      241 gcggccacgt cgtcaccaat cgcgagcccg ccgccaagtg gagctggaat gactggttca
      301 tcgacattgg tgtctccagc aagcaagaag ccactgatct cggcgtcggt cccggctgcc
      361 acgcgatctg gaacccgccg acgaaacgcc tgcgcgacgg ccggatcacc ggcaaggcca
      421 tggacgaccg ggcggcgctc gccatcgcaa cgctcgccgg agagcgcctg gccagtcgca
      481 cggatctcgc ctatgaggtc tggctcgtct ccacgatcca ggaagagaac ggtctgatcg
      541 gggcacaaag cgttgccgac gagatcgacc tggatctctg catcaacctc gacgtcggcc
      601 tgaccggtga tgtgccgggc gtcgacgtgc gggatttccc gtccaagctc ggtgccggcc
      661 cgatcgtggt ctaccaggat ggcagcgccc actattcccg ctcattgtcc gaccggctgg
      721 tggcgcttgc cgggcacaac gatattcccg tccagcgcgc ggtatttcag cagtacggga
      781 gcgatgcggc cgccctgctg aggcgaggcg tcgatgtatc gcttctgacg tatccgacgc
      841 ggtacaccca ctcgccgatc gaaacggtcg acgagcgcga tatcgagcat tgcgtcgacc
      901 tgatcgttgc gttcgccacg acggccgtct agtcgattcc tcgcgataca ctcccagcaa
      961 acgtgccgtg acgatcgcag ggagggccag agagcgagat gagggcgtgg ctctcgaacc
     1021 tgcgcttgcg cgtcctgctg ctggtgctcg tggctgtgct gccggcggtc ggattgctgc
     1081 tggtcacggt ctctgagcaa cgcgacgaag cggtccagaa tgccgaggcc gccgcgcgcc
     1141 agttagtcgg cctggctgca gccgatcagg gccgccaaat cgaaagtacg cgccagctcc
     1201 tgacggtcct cgcgcagttc cccgaggtcc agtcgggcgg gcaggcatgc aatgagctct
     1261 tcgccgatct cctgacccaa ttcccgcttt acgcgaatct cggcgtgatc ggactcgacg
     1321 ggatggtgga ctgcagcggc gtcccgaccg acggtccggt ctacctcggg gatcgcacct
     1381 acttccagcg cgttgttgcc tcccaggtct tcgtcgtcgg cgagtatcag accggccgga
     1441 tcaccgaaaa gccggcgctg aacaccggat atccggtaat caaggacaat aagctggtcg
     1501 gcgtcgttta cgcggcgatc gatttgaatt cgttggccca attcgcggcc caggcccgcc
     1561 tgcccgaggg cgccgtgctg accgtgctcg atcggtccgg gcgtgtcctg gcgcgagttc
     1621 cgccggagca gcaacttgtt ggccagtcgt tggtcggcac accggtcgtc gatacgatcc
     1681 tggcccaggg caccggcacg accgaacaga agctggatga tcagacctac gtcatcgcgt
     1741 tcgagtcgct tcgcagcgcc gagcaaggaa atgcctacct cagtctcgcg ctgccgaaat
     1801 cggacattac agcatcggcc gagaagcgct tcggcgataa cctgacgcgg ctcgggctgg
     1861 ccgtgatggt catcctgatc gcggcgtggg tggggacgga cctgctcgtg cggcgcagcc
     1921 cggagagcta tcgcgtcctc gtccgccgct actacgacgc cttctcgacc ggcggtctcg
     1981 atctgatgga ggaagtcgtc gccgatgact tcctcgacca cgacccgatg ccgggccagc
     2041 cggcggggct gctcgggctg aagcaggcag tcggcgagtt ccgcgcggcc ttcccggacg
     2101 gtgaaatggt ggtcgacgat ctggtcgccg agggtgaccg ggtcgtagcg cgagtcacga
     2161 tgagcggcac gcaccggggc gaatatgcgg gcatgccggg gaccggccgg gcggtccgcg
     2221 ccgaaggaat cgagatcttc cgggtggcgc atggcaagat cgccgaaggc tggagccgat
     2281 tcgtgctccc gctggccatg atcgaggacg cgccggacat cctcgcgaac ggcgagacga
     2341 gcgcggagat tgacctcgat gagtctgaaa ccgggcgaac cgggccaatc cgctcggtgc
     2401 tcgagtc
//
LOCUS       DASSGX010000413         3361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_615512, whole genome shotgun sequence.
ACCESSION   DASSGX010000413 DASSGX010000000
VERSION     DASSGX010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615512"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /locus_tag="VFI12_07330"
     CDS             <1..943
                     /locus_tag="VFI12_07330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET7056259.1"
                     /translation="SHHDTESTLAELKDDFTRIPLKDLGKRTVKKIGSDQITTLSGAF
                     AYNWTFSLPPLILLIVLIGALINRVTTVPVVENLRDLINRRAPAETQEMLLKLVDNAV
                     AQVSGGAASIGAIFTALLALWAASNAIGVLITGFNRAYEVQEDRSFIRKRAIQLGLTL
                     LLVLFVNVAFALLLFGQQIGRWLADWIGLGSQFDTIWSISRWPGAILGIMLMLAVLYW
                     AGPNVDQPFRWVSIGSVIATLAWLALVAGFNLYLDVSNPGTAYGVVGSLIVLLVFLNL
                     TGIIFFLGAEINAILFAAVADHPVGIFRGPAMAVANQ"
     gene            complement(954..1403)
                     /locus_tag="VFI12_07335"
     CDS             complement(954..1403)
                     /locus_tag="VFI12_07335"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET7056260.1"
                     /translation="MATIRIRADRQIDAPAAKLYRFLADYRTHHPAILPPAFSDFTVE
                     EGGVGAGTVIHFTVKAGGRTQTFRQRVSEPDPGRVLSETTIGTRNTTTFTVAPEGDGS
                     RVTILTEYEGQRGVQGLIERFLAPLLLRRLYTDELKRLDEYAHSRAA"
     gene            1560..>3361
                     /locus_tag="VFI12_07340"
     CDS             1560..>3361
                     /locus_tag="VFI12_07340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET7056261.1"
                     /translation="MQPSPPQTPRQPDGHAQHDATTHRGDLTDPHDIDHQAISPAEHD
                     GHAGHEAHGGHDTHAGHSVDMFRDRFWLSLLLSIPVVLYSEMVQNWLNFSMPSFPGSG
                     AIAPVLGTIVFLYGGTVFLKGGWDEIRSRTPGMMLLISLAIGVAFLASALTTFGLFDL
                     DFWWELVLLVTIMLLGHWQEMRAIGQAQGALSALAALLPDEAERITATGSEMVAVSDL
                     RTGDIILVRPGGRVPADGSIVDGQAELDESMITGESRPVDRAVGDHVVAGSVAVGSSI
                     RVRVEATGEGTTLAGIRRLVAEAQESRSRAQALADRAAAALFYVAVGAGLVTFVVWAA
                     IGDVEDAVTRAVTVLVISCPHALGLAIPLVIAISTAVSARQGILVKDRLALERMRLVD
                     TVLFDKTGTLTLGEHVVTDIASVNDDDDELLALAAAVESDSEHPVARAIVHSATAKDI
                     QIPKATGFRAIAGRGVEATIAGATVAVGGPAMVRERDLTIPSALAERTRSWSDRGAGV
                     LHVVRGGVIIGAVALEDQVRPESREAIDALHQQGVRVVMITGDARSVAEGVAADLGVD
                     DVFAEVLPEDKERTVAELQQRGAIVAMVGDGVNDA"
BASE COUNT          527 a         1161 c         1088 g          585 t
ORIGIN      
        1 gtcacaccac gacaccgaat cgacgctcgc cgagctgaag gacgacttca cgcggatacc
       61 gctgaaggac ctcggcaagc gcacggtgaa gaagatcggc agcgaccaga tcaccacgct
      121 ctcgggggcg ttcgcctaca actggacctt ttcgctgccg cccctgatcc tgctgatcgt
      181 cctgatcggc gcgctgatca accgggtgac gaccgtaccg gttgtcgaga acctgcgcga
      241 cctgatcaac cgccgtgccc cggcggagac ccaggaaatg ctgctcaagc tggttgacaa
      301 cgccgtcgcg caggtcagcg gaggcgccgc cagcatcggc gcgatcttca ctgcgctgct
      361 ggcactttgg gccgcctcga acgcgatcgg cgtcctgatc accggcttca accgcgccta
      421 cgaggtccag gaggaccgct ccttcatcag gaagcgagcc atccagctcg gactaacctt
      481 gttgctcgtc ctcttcgtga acgtcgcctt cgcgctattg ctcttcggcc agcagatcgg
      541 gcggtggctt gcggactgga tcggcctcgg ctcccagttc gacacgattt ggagcatctc
      601 ccggtggccg ggcgcgattc tcggcatcat gctgatgctg gccgtgctct actgggccgg
      661 ccccaacgtc gaccagccct tccgctgggt ttcgatcggc tcagtcatcg ctacgctggc
      721 ctggctggcg ctggtcgccg ggttcaacct gtacctggac gtctcgaacc cgggcacggc
      781 ctatggagtc gtcggcagcc tgatcgtgct gctcgtgttc ctgaacctga ccgggatcat
      841 cttcttcctc ggcgccgaga tcaacgcgat cctcttcgct gccgtcgccg atcacccggt
      901 cggcatcttc cgcggcccgg ccatggcggt cgcgaaccaa taaccgccgc gggtcaggcg
      961 gcgcgggagt gggcgtactc gtccagccgc ttcagctcgt cggtgtagag ccggcgcaag
     1021 agcagcggcg cgaggaatcg ctcgatcagc ccctgcactc cgcgctggcc ctcatattcg
     1081 gtcaggatcg taacccggct gccgtcgcct tccggcgcga ccgtgaaggt ggtcgtgttg
     1141 cgggtcccga ttgtcgtctc ggacagcacg cgcccggggt ccggctcgga aacccgctgc
     1201 cgaaaggtct gggtgcggcc accggccttg accgtgaaat ggatcacggt cccggccccg
     1261 acgccgccct cctcgaccgt gaaatcagag aaggcgggcg ggagaatcgc tggatgatgc
     1321 gtgcggtaat ccgccaggaa ccggtaaagc ttggcggccg gcgcatcgat ttgccggtcc
     1381 gccctgatcc ggatcgtagc catctgcata cctcctcgtt gccaggaccg cttccgccat
     1441 tgtggggccg agcccggctc cagcactgcg cacaggccac gcggcttgaa aaagataccc
     1501 cctcccccta tactcgtacg atggttaccc caccggggta taaggatcga gcgtaaccaa
     1561 tgcaaccatc tccacctcag actccgcgcc agccggacgg gcatgcgcag catgacgcca
     1621 cgacccaccg cggcgacctg accgatccgc acgacattga tcatcaggcc atctcgcccg
     1681 ccgagcacga tggccacgcc gggcacgagg cgcatggcgg ccacgatacg cacgccgggc
     1741 acagcgtcga catgttccgc gaccgcttct ggctctcgct cctgctctcg atcccggttg
     1801 tgctctacag cgagatggtc cagaactggc tcaacttctc gatgccatcg ttccctggca
     1861 gcggcgcgat cgctccggtc ctcggcacga tcgtcttcct ctacggcggg acggtctttc
     1921 tgaagggcgg gtgggacgag atccgtagcc gcacgcccgg catgatgctc ctgatttcgc
     1981 tggcgattgg ggtcgccttc ctcgccagcg ccctgaccac attcggcctc ttcgacctcg
     2041 atttctggtg ggagctggtc ctgctggtca cgatcatgct gctcggccac tggcaggaaa
     2101 tgcgcgcgat cggccaggcc cagggcgccc tgagcgcgct cgcggccctg ctcccggatg
     2161 aggccgaacg catcacagcg accggatctg aaatggtcgc cgtatccgat ctccggaccg
     2221 gcgacattat cctggtccgc ccgggcggcc gcgtcccggc cgacgggtca atcgtcgacg
     2281 gccaggccga gctcgacgaa tcgatgatca ccggcgaatc cagaccggtc gatcgcgccg
     2341 ttggcgatca cgtcgtcgcc ggctcggtcg cggtcggctc atcgatccgt gtccgggtcg
     2401 aggcgaccgg cgaagggacg accctggccg gcatccggcg gctggtggct gaagcgcagg
     2461 agtcgcgatc ccgggcgcag gccctggccg accgggccgc ggccgcgctc ttctacgttg
     2521 cggtcggcgc cggcttggtt acattcgtcg tctgggctgc gatcggcgac gtcgaggacg
     2581 ccgtcacgcg tgcggtcaca gtcctcgtca tctcctgccc ccacgccctg gggctggcca
     2641 ttccgctcgt gatcgcgatc tcgaccgccg tgtcggcgcg acaaggcatc ctggtcaagg
     2701 atcggctcgc gctggagcgg atgcgcctgg ttgatacggt ccttttcgac aagaccggca
     2761 cgctgacgct cggcgaacac gtcgtaaccg acatcgcctc tgtgaacgac gacgatgacg
     2821 agctgctggc cctggccgcg gccgtggaat cggacagcga gcaccccgtc gcccgcgcga
     2881 tcgtccactc agccacggcg aaggacattc agatcccgaa ggcaaccggg ttccgggcga
     2941 tcgccggtcg cggcgtcgag gccaccatcg ccggcgcgac ggtcgccgtc ggtggaccgg
     3001 cgatggtgcg cgagcgcgat ctcactattc catccgcgct cgcggagcgc acgcgatcct
     3061 ggtcggatcg gggcgcggga gtgctgcacg tcgtccgggg cggcgtcatc atcggtgcgg
     3121 tcgcgctcga ggatcaggtc cgcccggagt cccgggaagc gatcgatgcg ctgcatcagc
     3181 agggcgtgcg cgtggtgatg atcaccggtg acgctcgctc ggtggccgaa ggagtcgcgg
     3241 ccgacctcgg cgtggacgac gtctttgccg aggtcctgcc ggaagacaag gagcgcaccg
     3301 tcgctgagct gcaacaacgc ggcgcaatcg tggcaatggt cggcgacggc gtcaacgacg
     3361 c
//
LOCUS       DASSGX010000414         2265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_615517, whole genome shotgun sequence.
ACCESSION   DASSGX010000414 DASSGX010000000
VERSION     DASSGX010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615517"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /locus_tag="VFI12_07345"
     CDS             <1..1006
                     /locus_tag="VFI12_07345"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7056262.1"
                     /translation="GVLIDRYDCRIVLLIAQAGVMAVAAVFALLVGINLMHPWTMLIL
                     VAAYGSAMSFVFPSRTTIVPALVERADLTNAIALNAATQNLTRVIGPSLAGVLIALIG
                     VGGTFAVAAVLQIGALATTWRLPRSTGNRAAGRFDWASLSVGFRIVLADRFLMMLILM
                     ALAPTVLVMPYINLMPVFARDELGLGSTGLGVLLGAVGLGSVAGALAIAHSERLRDWS
                     HAQLVTATAFGAVVLAFALTPSVHLALPLLFVAGWTSAAYLALNQTVLQLHVDDAVRG
                     RVISVYMLTWGMLPVGQLLVGTLADATGTPVAVAIASALSILCVIAIARKSANEARAS
                     "
     gene            complement(1003..1623)
                     /locus_tag="VFI12_07350"
     CDS             complement(1003..1623)
                     /locus_tag="VFI12_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056263.1"
                     /translation="MVLIIGLAMCFAPSVSAQQDAPLTFTIHLAECPAGYQGDDPFGD
                     CHENRKAGISFQWSVAAPGAPEMVTTDSNGVAVIETIAAYGTLGISIIELPPHHLAGY
                     SVYCSGYDGAEAVPVNYIDGEVGIAYSTRTPVSAAVTCDWYNIPIAAQGGDDTGNGGD
                     QEPVTRLPSTGAGTLDVADLSALPAALITAGLIGTFAFGLRRRALR"
     gene            complement(1813..2142)
                     /locus_tag="VFI12_07355"
     CDS             complement(1813..2142)
                     /locus_tag="VFI12_07355"
                     /inference="COORDINATES: protein motif:HMM:NF013089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HET7056264.1"
                     /translation="MSTYGLLLAAIAIEVVATTALKASHGMTRLVPSVIVVVGYVVSF
                     YLVAILLKRMDLGLVYATWSALGTAAIAIIGVVIYDDALTIWRVTGLFLIIAGVMLLN
                     LSGGHAS"
BASE COUNT          394 a          780 c          682 g          409 t
ORIGIN      
        1 cggcgtcctg atcgaccggt acgactgccg gatcgtgctc ctgatcgcgc aggccggtgt
       61 catggccgtt gccgcggtct tcgcactcct cgtcgggatc aatctgatgc atccgtggac
      121 gatgctgatt ctggtcgcgg cttacggcag cgcgatgtcc ttcgtcttcc cgagccggac
      181 cacgatcgtg ccggcgctgg tcgagcgggc cgacctgacc aacgcaatcg cgctcaatgc
      241 cgccacccag aacctgaccc gcgtgatcgg cccgtcgctg gctggtgtgc tgatcgcgct
      301 gatcggcgtg ggcggcacct tcgcggtcgc cgccgtgctc cagatcggcg cgctggccac
      361 cacctggcga ttgccccgct caaccggcaa ccgcgcggcc ggcaggttcg actgggccag
      421 cctgagtgtc ggcttccgga tcgtgctggc cgaccgcttc ctgatgatgc tgatcctgat
      481 ggccctcgcc ccgaccgtgc tggtgatgcc ctacatcaac ctgatgccgg tcttcgcgcg
      541 ggatgagctg ggcctcggct cgaccggact cggcgtcctg ctcggggcgg ttgggctcgg
      601 ctcggtcgcc ggggcgctcg cgatcgcgca ctccgaacga ctacgggatt ggtcgcacgc
      661 gcaactggtg acggcgacgg ccttcggcgc ggtcgtcctc gccttcgcgc tgacgccgag
      721 tgtgcatctg gccctgccac tgctctttgt cgccggctgg acgagcgccg cctacctcgc
      781 cctcaaccag accgtgctcc agctccacgt cgatgacgcc gtacggggcc gggtgatctc
      841 ggtctacatg ctgacgtggg ggatgctgcc ggtcgggcaa ttgctggtcg gcaccttggc
      901 cgatgcaacc ggcacacccg tcgcagtcgc catcgccagc gccctctcga tcctctgcgt
      961 catcgccatc gcccgcaagt ctgccaacga agcgcgggcg tcctaacgca gggcgcgccg
     1021 ccgcaacccg aaggcgaacg taccgatcag ccctgccgtt atcagggcgg ccggaagtgc
     1081 ggagagatca gcgacatcca atgtgcctgc gccagtcgac ggtaatcggg tcaccggttc
     1141 ctgatcgccg ccattcccgg tgtcgtcgcc accttgggca gcgatcggga tgttgtacca
     1201 gtcgcaggtc actgccgcac tcacaggtgt acgggtcgag taagcaatgc cgacctcgcc
     1261 atcgatgtaa ttaaccggga ctgcttctgc cccatcatat cccgagcaat agacggaata
     1321 gccagcaagg tgatgcggcg gcagttcaat tatcgaaata ccaagcgtgc cgtacgccgc
     1381 aatcgtctca atgacagcca cgccgttgct atcagtcgtc accatctccg gcgcgccagg
     1441 ggcggctacg ctccattgaa atgaaatgcc cgccttgcgg ttctcgtggc agtcaccgaa
     1501 cgggtcgtca ccttgatatc ccgctgggca ctccgccaag tggatggtga acgtcaatgg
     1561 ggcatcttgt tgtgcgctta cagatggagc aaagcacatg gcgagcccga tgataagcac
     1621 catcgccacg agccccatgg cccaaccttt agtcctcatt gatgccctgc ctattcaacg
     1681 tcagtccctg ggatatgact gctccattgc ggattcgccc atcctgtgag tctacccctc
     1741 aaacgcatgg cggcagcagc acggtacctg ttaagacacc ctcgcgatct cgacccagcc
     1801 cggacgcgct gctcacgagg cgtgaccgcc ggagaggttg aggagcatca cgccggcgat
     1861 gatcaggaag aggccggtca cgcgccagat ggtcaacgcg tcgtcgtaga tcacgacacc
     1921 gatgatcgct atcgccgccg tgcccagtgc cgaccaggtc gcgtagacga gcccgagatc
     1981 catccgcttg agcaggatgg ccaccaaata gaaggaaacg acgtacccga cgactacgat
     2041 cacgctcggg accagccgcg tcatcccatg cgacgccttg agcgcggtcg tcgcgaccac
     2101 ctcgatcgca atcgccgcca gcaacaaccc gtacgtgctc atcgatccgc tcccgccatt
     2161 tgccaaacgc cacgcaatcg taccgccggg cgcacctccg aacgtagcgc ggtgcggaag
     2221 caccaaatcg cgggcgccgg tcccattccc aggatggaat gtcgc
//
LOCUS       DASSGX010000415         3302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_833804, whole genome shotgun sequence.
ACCESSION   DASSGX010000415 DASSGX010000000
VERSION     DASSGX010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_833804"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..1562)
                     /locus_tag="VFI12_07360"
     CDS             complement(186..1562)
                     /locus_tag="VFI12_07360"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HET7056265.1"
                     /translation="MVQAPTKGQLIAEFEEQFSSSKAMYERAKQSIAGGIAHDGRHQR
                     PFPLYISRAEGAYKYDLDGRRLIDYAMGHGALILGHGDPDVITAMHAQIDRGTHYGSG
                     HEGEVAWAEQIRSMIPSAERVRFVASGTEANLLAMRLARSYTGRNTVLKFEGHFHGWS
                     DYVVKSEKPPFENPAVAGVPDDVLRTVATVPANNIEMLTERLSQGDVAAILVEPSGAS
                     WSTIPLDADFLTKLRDAATQAGAVLIFDEVITGFRWSPGGAQKKFGIMPDMTTMAKIV
                     AGGMPGGAVAGKEEIMTFLEFKDEPGWGKRKIIHPGTFNANPLTAAAGLACLQKCADP
                     AVQEHCDRLASHLRSGFNVVLDKRGLPGCAWGASSVFHLILDQTPSERTPGDIAQPTG
                     INPETLKASGKAGRMAPLALAMQLEGVDLFSGGGMLSVRHTDDDVAFTVEAFDRALAR
                     LEQDGYFA"
     gene            1666..>3302
                     /gene="priA"
                     /locus_tag="VFI12_07365"
     CDS             1666..>3302
                     /gene="priA"
                     /locus_tag="VFI12_07365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HET7056266.1"
                     /translation="MDALITARFAEVAVDGTLPAGDSATLTYLAPPGCETAIQPGALV
                     WVPLRKKAALGIVMSVHDQEPAFEVKPIYEVAPGDRLEEGQLEFGRWLSHETASSLFG
                     CLSLMLPPGVSHSVTPWFELKSRAPGKTKMQERVLALLRVNGAMSLEQLQKELDSSLT
                     SVLPDLERAGLVSRRYQSEARTTRRRTERWWVAANDADPAKLTGRQRDLFDVVVGAGS
                     AGIRASEATDRTGVSAPVAKKLIDSGAILAEERPIERNANIEPWGTIPTLNPEQQAAW
                     AGIEAELRRRIGRTQVIFGVTGSGKTELYLRAIAATLRQGKQAIYLLPEIALTTHIAH
                     RVRERFPGRVAVLHSGLAMGARQEAWDRIGSGEKPVVVGARSALFAPAPDLGLIILDE
                     EHDPSYKQDVDPRYDARTAAEEIARRTGAVLILGSATPRTETLWRANSGESDMYRLRK
                     RAVATAPDLPPVQIVDLRLELQSGHTSLISRPLEQAIRAATERGEQTMLLLNRRGMAT
                     VVLCRSCGLARICPNCSIPLVYHEDRGALLCHRCDHR"
BASE COUNT          534 a         1063 c         1123 g          582 t
ORIGIN      
        1 cgcagggcga tgaacgttcc cgaccgggcc ggttcccggt gctcgcaacc cacccttcgg
       61 gcgaactatc gtggcctgcg gccacagcgg ggcatgcccc ccatctggct tccccccgtt
      121 cggtcggggg gaagaacttc cccgcgctac gtatgcgcac cggcttgaca tcgctcggct
      181 cgcggtcagg cgaaataacc gtcctgctca aggcgagcca gcgcgcgatc gaaggcctcg
      241 accgtgaatg cgacgtcatc atcggtgtgg cggacgctca gcatgccacc gccgctgaag
      301 agatccaccc cttcgagctg catcgccagc gccagcgggg ccatgcgacc ggccttaccg
      361 ctcgccttga gtgtctccgg gttgatgccg gttggctggg cgatgtcgcc gggcgtccgc
      421 tctgacggcg tctggtcgag gatgaggtgg aagaccgagc ttgcgcccca tgcgcatccg
      481 ggcagcccgc gcttgtcgag cacgacgttg aagccgctgc gaaggtgcga ggcgagccgg
      541 tcgcagtgtt cctgaacggc cggatcggcg cacttctgga ggcaggcgag accggcggcg
      601 gcggttagcg ggttggcgtt gaaggtgccg gggtgaatga tcttgcgttt gccccagccc
      661 ggttcgtcct tgaactcaag gaaggtcatg atctcttcct tgccggcgac ggcgccgccc
      721 ggcatgccac ccgccacgat cttggccatg gtggtcatgt ccggcatgat gccgaacttc
      781 ttctgtgccc cgcccggcga ccagcggaag ccggtgatca cctcgtcgaa gatgaggacc
      841 gccccggcct gcgtcgcggc gtcgcgcagt ttggtcagga aatcggcatc gagcgggatc
      901 gtgctccacg acgcgccgct cggctcgacc aggatcgcgg ccacatcacc ctgtgatagc
      961 cgctcggtca gcatctcgat gttgttcgcc gggaccgtcg cgacggtccg cagcacgtca
     1021 tccggcaccc cggcgacggc cgggttctcg aacggcggct tctcgctctt gacgacgtag
     1081 tcgctccagc cgtggaagtg cccctcgaac ttgaggactg tgttccggcc ggtataggag
     1141 cgggcaagtc gcatcgcgag cagattcgcc tccgtcccgg atgcgacgaa acgcacccgt
     1201 tcggcggacg ggatcatcga ccgaatttgc tcggcccagg caacttcgcc ctcatggccg
     1261 gagccgtagt gcgtgccccg gtcgatttga gcatgcatgg ccgtgatcac gtcggggtcg
     1321 ccatgcccga ggatcagcgc gccgtggccc atcgcgtaat cgatcaggcg gcgaccgtcg
     1381 aggtcatact tgtaggcgcc ttcggcccgg ctgatgtaga gcggaaatgg tcgctggtgg
     1441 cggccgtcgt gggcgatgcc gcccgcgatg ctctgctttg cccgctcata cattgccttc
     1501 gaagaggaaa actgctcctc gaattccgca atcaactgac ctttcgtggg cgcctgaacc
     1561 atggagtgag cctcttttcc gccgtgcatg gggacgtgtc gctgccggga agtataggac
     1621 cgttgcccgc ccggcaaatt gacggaatat cgtacgcttg ttcgcatgga cgcgctgatt
     1681 acagctcgat ttgccgaggt tgcggttgac gggaccttgc ccgccggcga ttccgcaacc
     1741 ttgacgtacc tggcgccacc cggttgtgag acggcgattc agcccggcgc gctggtctgg
     1801 gtaccgctcc gaaagaaggc ggcgcttggc atcgtcatgt ccgtgcacga ccaggaaccg
     1861 gcgttcgagg tcaagccgat ctatgaggtg gcgcccggtg accggttgga ggaaggccaa
     1921 ctcgaattcg ggcgctggct gagtcatgag acggcgtcga gtctgtttgg atgcctgtca
     1981 ttgatgttgc cacccggcgt gtcgcactcg gtcacgccct ggtttgagtt gaagagccgc
     2041 gcgccgggca agacgaagat gcaagaacgc gtcctcgcgt tgctgcgcgt gaacggcgca
     2101 atgtcgcttg aacaactcca gaaggagctg gattcgtccc tgacgagcgt tctgccggac
     2161 ctcgaacggg caggactggt ctcacgccgg taccagtcgg aggcgcggac gacgcgacgg
     2221 cgcacggagc ggtggtgggt cgccgcgaat gacgctgatc ccgcgaagct gaccggtcgg
     2281 cagcgggacc tgttcgatgt cgtggtcggc gctggatcag ccggcattcg cgccagcgag
     2341 gcgaccgatc ggaccggcgt ctcggctccg gttgcgaaga agctcatcga ctcgggcgcc
     2401 atcttggccg aggagcgccc gattgagcgg aatgccaaca tcgagccgtg gggtacgatc
     2461 ccgacgctca atccggaaca gcaagcggcg tgggcgggaa tcgaggcgga gctgcggcgg
     2521 cggatcgggc gcacgcaggt catcttcggc gtcaccggca gcggcaagac tgagctctac
     2581 ctccgcgcaa tcgcagcgac gctccggcag ggcaagcaag cgatttatct gttgccggag
     2641 attgcgctga cgacgcacat cgcgcatcgc gtccgggaac gcttcccggg ccgggtcgcc
     2701 gtgctgcatt ccgggctggc gatgggcgcg cggcaggagg cgtgggaccg gatcggttcc
     2761 ggcgagaaac cggtcgtggt cggcgcacgc tcggcgctct tcgcaccggc gcccgacctc
     2821 ggcctgatca tcctcgatga agagcacgac ccgtcctaca agcaggatgt cgaccctcgc
     2881 tacgacgcgc ggacggcagc tgaggagatc gcgcgacgca ccggcgccgt cctgatcctg
     2941 ggtagcgcca cgccgcgcac agagacgctc tggcgggcga actcgggcga atcggacatg
     3001 taccgcctcc gaaagcgtgc cgtcgccacc gcgccggatt tgccgccggt gcaaatagtc
     3061 gatctccgcc tcgaattgca gtcgggccac acctcgctga ttagtcgccc gctggaacag
     3121 gcgatccggg cggccaccga gcgtggcgag cagacgatgc tgctcctgaa tcggcgtggc
     3181 atggcgacgg tcgtgctctg ccggtcgtgc ggattggccc ggatttgccc gaactgctcg
     3241 atcccgctcg tctaccacga ggaccggggc gcgctcctct gccatcgctg tgatcatcgg
     3301 ga
//
LOCUS       DASSGX010000416         3965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_506417, whole genome shotgun sequence.
ACCESSION   DASSGX010000416 DASSGX010000000
VERSION     DASSGX010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506417"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VFI12_07370"
     CDS             <1..528
                     /locus_tag="VFI12_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056267.1"
                     /translation="SNLPVRVAFPILVSNLLTDLASQPIPASLSVGDPITIAPHAGAA
                     SLEITDPGGVAHTLTVQPVSATGAAQDLTYADTGASGPYLLRELDANGQPIQAAMIMV
                     NAGHPQESDLAPNPLLAQALHSGSVEAEGSRLRDQIALWPVVIAAVLALLLVEWLLTL
                     RQERRRLPMAAGTRP"
     gene            525..3182
                     /locus_tag="VFI12_07375"
     CDS             525..3182
                     /locus_tag="VFI12_07375"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET7056268.1"
                     /translation="MTPLAAIQFAFGRERALLLLLLVPLLGFISWRTAWRSKRLRKSV
                     LLLRLAMLALLIAALAEPLAARTGTASTTVILMDRSTSVSSNADSGINAWLTDALASA
                     GGADNAAIVAFGGSADLAVPSGPASQINQDWADGIDTSAIDPAFTNIESAVALARSLP
                     VGGNRRIVLVSDGAENLGAVRNQVAQAARDGVPIDVVKVAGAGNDDLRIDSVTAPAAI
                     WQGEQPNVLVSVSTVTAGPAHLELVIDGGVVAGQDANLAAGLSSFSFAVPALDPGFHS
                     VAVRVSGDATMDQFTENDVAPLALIVRDAPRVLLVVAVGSDPSRLSNALAARGADVTN
                     ATPEQVPVQMSALSTYDAFVLDNVPASALQVEQIAGLQEATRSLGKGLIVIGGTSSYG
                     PGQYAGTRLEDMLPVTVKVTDGRQRQRVALLLVVDHSGSMSYDPLHESSKMAMAKEAI
                     RLAGNALSDGDTIGVLAFSDSQEWTFPLTQINGDATRQKMNAAVNQLDATGGTEIYPA
                     LQVGLDAIRNVDADVRHVILLSDGKSKSGTRDAYIKLVSDAGSDRTSVSTIAIGNDAD
                     TQLLQAIAQAGGGRYHFTDKPDDIPQLTLEEARSAGAQSIIRGDFQPVQTLPSPIMTG
                     LDPRALPPLDGYDFTEARPSAQVVLVSHRNDPVLAKWQYGLGRVAAWTPDDGADLASQ
                     WGSWEKYDQFWAAMLRWTLPDPENRPVSTDVSRDGPDVVLSLSTTSAASANDYVDLSA
                     MTARITGPGGAVTDNIPLTESGSGQYQVRIRNADSGAYKLELVNGSGAVQGGAYGFTL
                     PGSPELLPAPGGDQLLTSIASATGGRVLSMDKPGDLFRAPASTGETLRTYRPIWTWAV
                     VAALLFFLAELMLRLNGYARLRSLRSRLA"
     gene            3343..>3965
                     /locus_tag="VFI12_07380"
     CDS             3343..>3965
                     /locus_tag="VFI12_07380"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HET7056269.1"
                     /translation="MIRVKVHAEGTVRDPNLPLEQISDALQSDAALVWVDVVAPTADD
                     LALLEEEFGFHHLALEDASLVHERPKIDEYDDFLLIIFYALTHGAANPGLRLEQLSIF
                     AGRNYVVTLHQNDLAVLEETSGRWSASHAKIKDGASGLLVYAILDAMVDAYLPVVDRM
                     SDRIDQIEDAVFGRFDASAQQEIFHLKRDLLMMRRVLAPERDVLSFL"
BASE COUNT          633 a         1330 c         1325 g          677 t
ORIGIN      
        1 tcgaatctgc cagtgcgggt tgcctttccg attctggtct cgaacctcct cacggatctc
       61 gcgtcgcaac cgatccctgc cagcctctcg gtcggcgatc cgatcacgat cgcgccccac
      121 gccggggccg cctcgctcga aatcactgat ccggggggcg tcgcgcacac cctgacggtc
      181 caacccgtgt ccgctacggg agccgcgcag gatctgacct atgcggatac cggcgcatcc
      241 gggccgtacc tgcttcgcga gctggacgcg aacgggcagc cgatccaggc ggcgatgatc
      301 atggtcaatg ccggccatcc gcaggagtcg gacctggcgc ccaatcccct gctcgcccag
      361 gcgctgcact ctggctctgt cgaggcagag ggttcgcgat tgcgtgacca aatcgcgctc
      421 tggccggtgg tgattgccgc cgtgctggcg ctgctcctgg tcgagtggct gctgacgctt
      481 cggcaggagc gacgccgctt gccaatggcc gcgggaaccc ggccatgacc ccgctcgcgg
      541 ccatccagtt cgcctttgga cgggagcgag cgttgctcct gctcttgttg gtcccgctgc
      601 tggggttcat ctcgtggcgg acagcgtggc ggtccaagcg gctgcgaaag tccgtcctgc
      661 tgctccgcct ggcaatgctg gcgctgctga tcgccgcgct ggccgagcca ctcgcggctc
      721 ggaccggcac cgcgtcgacg accgtcatcc tgatggaccg ctcgaccagc gtcagctcga
      781 acgcggacag cgggatcaac gcctggctga cggatgcatt ggccagtgcg ggcggggcgg
      841 acaacgccgc gatcgtggcc ttcggtggtt cggcggacct ggcggtaccc tccggccccg
      901 catcgcagat caaccaggac tgggcggacg ggatcgatac ctcggcgatc gaccccgcct
      961 ttacgaacat cgaatcggcg gtcgccctgg cgcggtcgtt gccggtcggc gggaatcggc
     1021 ggattgtgct ggtctcggac ggcgccgaaa atctcggcgc ggtcaggaac caggtggccc
     1081 aggccgcgcg ggacggcgtg ccgatcgatg tcgtcaaggt ggcaggcgcc ggtaacgacg
     1141 atttgcgcat cgatagcgtg accgcgccgg ccgcgatctg gcagggtgag cagccgaacg
     1201 tactggtcag tgtctcgacg gtgacggccg gtcccgcgca cctcgagctg gtaatcgacg
     1261 ggggcgtagt ggccgggcag gacgcgaacc tggcggccgg gctgtcgagc ttctcatttg
     1321 ccgtgcccgc gctcgatccc gggttccaca gcgtggcggt gcgcgtgtcc ggcgatgcga
     1381 cgatggacca gttcacagag aacgacgtcg cccccctggc gttgatcgtc cgcgatgcgc
     1441 cacgagtgct gctggtcgtc gccgtggggt ccgatccgag ccggttgagc aacgccctgg
     1501 cagcccgtgg ggccgacgtc acgaacgcga cgcccgagca ggtgccagtc cagatgtcgg
     1561 cgttgagcac ttacgacgcc ttcgtgctcg acaacgtgcc ggcgagcgcg ctccaggtcg
     1621 aacagatcgc cgggctgcag gaggcgacgc gcagccttgg caaggggctg attgtgatcg
     1681 gcggcacctc cagctacggc cccggccagt acgcggggac ccggctcgag gatatgctgc
     1741 cggtgaccgt caaggtgacg gacgggcggc aacggcaacg ggtcgccttg ctgctggtgg
     1801 tcgaccacag cggttcgatg tcctatgacc cgctccacga gtcgagcaaa atggcgatgg
     1861 ccaaggaggc gatccggctg gccggcaacg ccctgtccga cggcgacacg atcggagtgc
     1921 tcgcgttctc cgattcgcag gaatggacgt tcccgctcac ccagatcaac ggcgacgcca
     1981 cccggcagaa gatgaacgcg gcggtcaacc agctggatgc gaccggcggc accgaaattt
     2041 atcctgcctt gcaagtcggg ctggacgcca ttcgcaatgt cgatgccgat gtccggcacg
     2101 ttatcctgct ctccgacggc aaatccaagt cggggaccag ggacgcctac atcaagctcg
     2161 tctccgacgc tggctccgat cgcacctcgg tttcgacgat cgcgatcggc aacgacgcgg
     2221 atacgcagct tttgcaggcg atcgcgcagg ctggcggcgg acgctaccac ttcaccgaca
     2281 agccggatga cattccgcaa ctcacgctcg aggaggcccg cagcgccggc gcgcagtcga
     2341 tcatccgagg cgatttccag ccggtgcaaa ccctcccctc cccgatcatg accggacttg
     2401 atccccgcgc attgccgcca ctcgacggct acgacttcac cgaggcccgt ccgagcgcgc
     2461 aggttgtcct ggtcagccat cgcaacgatc ccgtgctggc caagtggcaa tacggcctcg
     2521 gccgggttgc cgcctggacc ccggacgacg gcgcggatct ggccagccag tggggctcgt
     2581 gggagaagta cgaccagttc tgggcggcca tgctgcgctg gaccctgccc gacccggaga
     2641 accggccggt gtcaaccgac gtcagccggg atggcccgga tgtcgtcctc tcgctgagca
     2701 cgaccagtgc tgcctcagcc aatgattatg tcgacttatc agccatgaca gcgcgcatca
     2761 ccggtcccgg cggcgcggtc accgacaata ttccgctcac ggagtccggc tcgggccagt
     2821 accaggtgcg catccgcaac gccgattcgg gcgcctacaa gctggagctg gtcaatggca
     2881 gcggggctgt acaaggcggg gcgtacggtt tcaccctgcc cggctcgccg gagttgctcc
     2941 ccgcgccggg cggtgatcag ctcctgacct cgatcgcgtc cgcgacgggc ggccgggtcc
     3001 tgagcatgga caagccgggc gacctcttcc gggcgccggc gagcaccggc gagacgctgc
     3061 ggacctaccg gccgatctgg acctgggcgg tcgttgctgc cctgctcttc ttcctcgccg
     3121 agctgatgct ccgcctcaac gggtatgcgc gcctgcgctc gctccgcagc cgcttggcct
     3181 gactcccata cggcgtttga ccgtccagac gtcataccgg gcggctgcgc ccggaatggc
     3241 gtctcttgcg cggtgctgcc tgcgagtcat gcgaagcggg ccgctccgcg ctacatttgc
     3301 cgagtctgac tggcgattga tctggacggg agtggacagg gcgtgatcag ggtcaaagtg
     3361 cacgcggaag gcacagttcg cgacccgaac ctcccgctcg agcagatcag cgatgccctg
     3421 caatcggatg ccgcgttggt ctgggtcgat gtcgttgccc cgacggcaga cgatctggcg
     3481 ctgctcgagg aagagttcgg gtttcaccac ctggcgcttg aggatgcgtc gctggtccac
     3541 gagcggccga agatcgacga gtacgacgac ttcttgctga tcatctttta cgcgctgacc
     3601 cacggcgcgg ccaatccggg actccgcctc gaacagctct cgatcttcgc cggccggaac
     3661 tacgtcgtca cgctccatca gaacgatttg gcggtcctcg aggagacgag cggccgctgg
     3721 agcgccagcc acgcaaagat caaggatggc gcctcaggct tgctggtcta cgcgatcctc
     3781 gacgcgatgg tcgatgccta cctgccggtc gtcgaccgga tgtctgaccg catcgaccag
     3841 atcgaggacg cggtgtttgg ccgcttcgac gcaagcgcgc agcaggagat cttccacctc
     3901 aagcgggatc tgctgatgat gcggagggtc ctcgcgccgg agcgggacgt gctcagcttt
     3961 ctgat
//
LOCUS       DASSGX010000417         2810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_289010, whole genome shotgun sequence.
ACCESSION   DASSGX010000417 DASSGX010000000
VERSION     DASSGX010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289010"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            277..879
                     /locus_tag="VFI12_07385"
     CDS             277..879
                     /locus_tag="VFI12_07385"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7056270.1"
                     /translation="MATQVAPCAAFLRSRHSLFALLAACALIVVASPARAADPAIEFM
                     KQTARELIAAARTGSQRDFMEVVQNRGHIPAIGLYALGDYKAQLPASERDSYYGGMVR
                     FIARYASTEAPKYPVSHAEILSPVVREGNAIYVDSRVHLQTGTVYDVRWLLVPQGNSF
                     RVRDAQVMGFWMSPFLKNLFETYIGENGGNPRALVIALSR"
     gene            complement(991..1935)
                     /locus_tag="VFI12_07390"
     CDS             complement(991..1935)
                     /locus_tag="VFI12_07390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET7056271.1"
                     /translation="MHTHSHSHAHAHIGVDAGDRRVMWAVLVNVALTVGQVIGGILAG
                     SLALIADAVHNLSDAMSLGIAFAARKIARRESDPRMTFGYVRAEIVAALINYTTLILI
                     GLYLLYEAVARFYSPQDVEGWLVVAVAGLALAVDAVTVLLTYTLAKTSVNIRAAFLHN
                     LADAIGSIGVIAAGVLIILFGWTIVDPIVTMVIAVYILWLAGSEIGGVVRILMMGVPP
                     EIDVEELVAAIRKVEGVTDVHHVHVWAIDEHRNSLEAHIVVPDEAAGESKAIKDRVRV
                     LARDRFGVTHTTLEIERASECHEGADASTIGHQIEHRR"
     gene            complement(2034..2741)
                     /locus_tag="VFI12_07395"
     CDS             complement(2034..2741)
                     /locus_tag="VFI12_07395"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7056272.1"
                     /translation="MVLEAEGLVKGFDDRLLIDNLSFTLPPGGIVGVIGPNGAGKTTL
                     FRMITGQEQPDAGTIRLGETVKLGYVDQSRQALDDKKTVWEEISGGYDMIQLGKREVP
                     SRAYVGWFNFRGADQQKKVGMLSGGERNRLHLAKMLKEGSNLLLLDEPTNDLDTETLS
                     ALESALEDFPGCAVVISHDRFFLDRIATHILAFEGDSHVEWFEGNFEAYEEDKKRRLG
                     EAAVEPHRIKFKRFERA"
BASE COUNT          473 a          926 c          899 g          512 t
ORIGIN      
        1 gtgcaggaag acgaggggcg tgccccgccc agtctcctca tagtaaacct gagttcctcc
       61 gacgtcggcg taaggcatga gtcccatccc ttctggtcaa atcggtaacg gttggtacac
      121 tgtgaccgtg gagacatgac gtgtgcaagg cttctccggt atgagcctgc cgcagttgac
      181 ggggagagca acggctgcca attgatgccg tgtgcttggc tgctcgaggc ccggaacatg
      241 tcgatgttgg tccgcttctt gttcgggaga tatggaatgg ccacccaggt tgccccgtgt
      301 gcagcgttct tacgcagccg gcacagctta ttcgcgttac tcgctgcatg cgctctgatc
      361 gtcgtcgcga gcccggcccg cgctgcggat cccgccatcg agttcatgaa gcagaccgcg
      421 cgcgagctca tcgccgccgc caggaccgga tcgcagcggg acttcatgga ggtggttcag
      481 aaccgcggcc acatacccgc catcgggctt tacgcgctcg gcgattacaa ggctcagctg
      541 ccggcgtcgg aaagggatag ctactacggc ggcatggtcc gcttcatcgc gcgttacgcc
      601 tcgacggagg cgccaaagta ccccgttagc cacgccgaga tcctctctcc ggtggtccgc
      661 gaaggcaatg cgatctacgt cgacagccgc gttcacctgc agaccggcac cgtctacgac
      721 gtccgctggc tgctcgtgcc gcagggcaat tcgttccgtg ttagggatgc tcaggtcatg
      781 ggcttctgga tgagcccgtt cctcaagaac ctgttcgaga cctatatcgg cgagaatggc
      841 ggcaaccctc gcgcgctggt gattgcgctc agccggtgat cggcgccgga ctttcggcgc
      901 tcaatccaat ttcatccgtc atcccggcgc ttgtcgccgg gggccgggca caaggcccgg
      961 catgactgag gtcgcttgaa tgcagtagca tcatcggcgg tgctcgatct ggtggccgat
     1021 ggtgctggcg tcggccccct cgtgacactc cgaagcgcgc tcgatctcga gcgtcgtgtg
     1081 cgtgacgccg aagcggtccc gggcgaggac cctgacccga tccttgattg ctttggattc
     1141 cccggccgct tcgtccggca cgacgatgtg ggcctcgagg gagttgcggt gctcgtcgat
     1201 ggcccagacg tggacgtgat gcacatccgt tacgccctcg accttgcgga tggccgcgac
     1261 gagctcctcg acatcgatct ccggcggcac gcccatcatg aggatgcgga cgactccgcc
     1321 gatctcggac ccggcgagcc ataggatgta cacggcgatc accatcgtca cgatcggatc
     1381 gacgatcgtc cagccgaaca gaatgatcag cacgccggcc gcaatgacgc cgatcgagcc
     1441 gatcgcatcc gccagattgt gcaggaaggc agcgcgaatg ttcacgctcg tcttcgcgag
     1501 cgtgtaggtc agcagaaccg tcacggcatc gaccgccaag gccaggcccg caacggccac
     1561 cacgagccag ccctccacgt cctgcggtga gtagaaccgc gccacggcct cgtagagcag
     1621 gtaaagcccg atcaggatca gcgtcgtata gttgatgagc gcggcgacga tctcggcacg
     1681 gacgtagccg aaggtcatgc gggggtcgct ttcccgccgg gcgatcttgc gggcggcaaa
     1741 tgcaatgccg agcgacatcg catccgacag gttgtgaacc gcatccgcga tcagcgcgag
     1801 gctgcccgcg aggatgccgc cgatcacctg ccctacggtg agcgccacat tgacgagcac
     1861 cgcccacatc acgcgccggt cgccggcatc gacgccgatg tgcgcgtggg cgtgcgaatg
     1921 ggagtgggta tgcaaggcca tcccctgtca gacgcgcatc ggcgccggcc cgatccggag
     1981 ccggcgcacc atgggcttcc gccgcagccc ggcgggggtg accgacgtgc ggcttacgcc
     2041 cgctcgaaac gcttgaactt gatgcggtgc ggctcgactg ccgcctcgcc cagccgccgc
     2101 ttcttgtcct cctcgtaggc ctcgaagttg ccctcgaacc actcgacgtg gctgtcgccc
     2161 tcgaaagcca ggatgtgggt cgcgatgcgg tctaggaaga agcggtcgtg gctgatgacc
     2221 acggcgcagc ccgggaagtc ctccagcgcc gattcgagtg ccgacagcgt ctccgtgtcg
     2281 aggtcgttcg tcggctcgtc gagcagcagc aggttgctgc cctccttcag catcttggcc
     2341 aggtgtaggc ggttgcgctc gccacccgag agcatgccga ccttcttctg ctggtcggcc
     2401 ccgcggaagt tgaaccagcc gacgtaggcg cgcgatggca cctcgcgctt gccgagctgg
     2461 atcatgtcgt atccgccgga aatctcctcc cagacggtct tcttgtcgtc gagcgcctgg
     2521 cgcgactgat ccacgtagcc gagcttcacc gtctcgccga gccggatcgt gcccgcatcg
     2581 ggctgctcct ggcccgtgat catgcgaaac agggtcgtct tgccggcacc gttggggccg
     2641 atgacgccga cgatgccgcc cggcggcaaa gtgaacgaca ggttgtcgat cagcagccgg
     2701 tcgtcgaagc ctttgacgag accttcggcc tcgagaacca cgctgccgag ccgcggcccg
     2761 ggtggaatct ggatctgcgc gttcccgacc tgctcgcgac cagcctcgtc
//
LOCUS       DASSGX010000418         2086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1161449, whole genome shotgun sequence.
ACCESSION   DASSGX010000418 DASSGX010000000
VERSION     DASSGX010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161449"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1546
                     /locus_tag="VFI12_07400"
     CDS             <1..1546
                     /locus_tag="VFI12_07400"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7056273.1"
                     /translation="ISLAIALASLSLFFFLGRDYFPEIRSGVIQMHMRAPLGTRIEVS
                     GRIATLVANSVEELLPGKVENIVSNCGLPVGAHNLAFIPTPTIGSQDCDLTILLKDEK
                     SPVWDYRRILRKGLTERYPGTEFTFQPSDLTAKILNFGAPAPIDVQINGPDSYPNYEF
                     ARKLAGQLRRIPGATDVVIQQTMRTPTLMVEGNRTFGLGVNRTEKDIASNLLMTTAGS
                     QQVDQVYWLDPSTGMSYLINVYTPQPQINSLNSLKTIPVESSSNGSNEHTDHDVQLLG
                     NLASLSAEGTPGVITHANIMPLFDIYVSTEGRDLGGVLTDVEHVVQSMTADLPRSAAI
                     EIHGQAALMHDAYFELIFGLLAAIGLVYLLIVVNFQSWLDPFIIITALPGALAGIAWS
                     LFLTHTRLSEPALTGAIMTMGTGTANAILVVSYARERLQEHGDALRAAIEAGKTRLRP
                     VLMTASAMIIGMVPMSIGYSQNAPLGRAVIGGLLVATVFTLFFVPCVYAMIYSRRPAR
                     QKGPAS"
     gene            1543..>2086
                     /locus_tag="VFI12_07405"
     CDS             1543..>2086
                     /locus_tag="VFI12_07405"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HET7056274.1"
                     /translation="MMNRFNGKRLAFIAIVLLVLYVGHRIYESSGDAALLREKTLEEA
                     IPTVAVVSPKPGAPTETITLPGNIVGWYEAPMYARVTGYVKMWYKDYGDQVKKGDILA
                     EINAPDLDAEYAQAKADLETQRVRYRLAEVTAKRWVALRPNHAVSEQSITVKEQELKA
                     EAAMVKAAEQKIRNIDAFIRF"
BASE COUNT          412 a          665 c          610 g          399 t
ORIGIN      
        1 gatctcgctg gcgatcgcac tggcatccct gtctctcttt ttcttcctcg ggcgcgatta
       61 cttccccgag atccggtcgg gggtcattca gatgcatatg cgggcgccgc tcgggacgcg
      121 tatcgaggtg tcaggccgca ttgccacgct cgtcgccaac agcgtcgaag agttgctccc
      181 cgggaaggtc gaaaacatcg tcagtaattg cggcctgccg gtcggcgccc ataacctcgc
      241 cttcattccg acgccgacga tcggctcgca ggactgtgat ctgacgatcc tcttgaagga
      301 tgaaaagtcg ccggtgtggg actaccgacg cattctccgc aagggcttga cggaacgtta
      361 tccgggcacg gaattcacgt tccagccatc cgatctgacc gccaaaattc tcaacttcgg
      421 cgccccggca cccatcgacg tgcagatcaa cggcccggac agttacccca actacgagtt
      481 cgcccgcaag ttggcgggcc aattgcgccg gatccctggg gccacggacg tcgtgatcca
      541 gcagaccatg cgcacgccga ccctcatggt cgaaggcaac cgcacattcg gcctcggggt
      601 caaccgaacc gagaaggaca tcgcctccaa tctgctcatg acgaccgccg ggagccaaca
      661 ggtcgaccag gtctattggc tcgatcccag cacgggtatg tcgtacctga tcaatgtcta
      721 tacgccacag ccgcagatca atagcctcaa tagtctgaag accatcccgg tcgagtcctc
      781 gagcaatggg tccaacgagc ataccgatca tgatgtgcag ttgctcggca atttggccag
      841 cctgtccgcg gagggcacgc cgggcgtgat tacgcacgcg aacattatgc ccctgttcga
      901 catctatgtt tccaccgaag gacgcgacct cggcggcgtg ctcacggatg ttgaacacgt
      961 ggtccagagt atgacggccg acttgccgcg cagcgccgcg atcgaaatcc atggccaggc
     1021 cgccctcatg catgacgcgt atttcgagtt gatcttcggc ctgctcgccg caatcgggct
     1081 ggtctacctg ctgattgtcg tcaacttcca atcctggctc gaccccttca tcatcattac
     1141 ggccttgccc ggcgcgttag cgggcatcgc ctggtccctg ttcctgaccc atacgcggct
     1201 gtcggagccc gcgctgacgg gcgccatcat gacgatgggc acgggaaccg ccaatgcgat
     1261 tctcgtcgtg tcctatgcgc gcgagcggct gcaagagcat ggggacgcgc tgcgggcggc
     1321 gatcgaagcc ggcaagaccc gcctccggcc ggtgctcatg acggcctcgg ccatgatcat
     1381 cggcatggtg ccgatgtcga ttggctattc gcagaatgcg ccgctggggc gcgccgtcat
     1441 cggcggcttg ctcgtggcca ccgtcttcac cctgtttttc gtgccctgcg tctacgcaat
     1501 gatctacagc cggcgaccgg ctcgacaaaa aggaccagcc tcatgatgaa cagattcaat
     1561 ggcaaacgtc ttgcctttat cgcaatcgtc ttgctcgtgc tctatgtcgg ccatcggatt
     1621 tatgagagca gcggcgatgc cgcgctgctg cgcgagaaga cgctcgagga ggctatcccc
     1681 accgtggccg tcgtctctcc caagccgggg gcgccgaccg agaccattac gctgcccggc
     1741 aatattgtgg gctggtacga ggcgccgatg tacgcccgcg tcaccggcta tgtgaagatg
     1801 tggtacaagg actacggcga ccaggtcaag aaaggggaca tcctcgccga aatcaacgcg
     1861 ccggatctcg acgccgaata tgcgcaggcc aaggcggatc tggaaacgca gcgcgtccgg
     1921 tatcggctcg ccgaggtgac cgcaaagcgc tgggtggcgc tccgccccaa tcatgccgtc
     1981 tccgagcagt cgatcacagt gaaagaacag gagttaaaag ccgaagcggc gatggtcaaa
     2041 gccgcggaac aaaagatccg taacatcgac gcgttcattc ggttca
//
LOCUS       DASSGX010000419         3969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_399232, whole genome shotgun sequence.
ACCESSION   DASSGX010000419 DASSGX010000000
VERSION     DASSGX010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399232"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..749)
                     /locus_tag="VFI12_07410"
     CDS             complement(18..749)
                     /locus_tag="VFI12_07410"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET7056275.1"
                     /translation="MVSGMSDTDPSPRQIKLLAVSDQVDPRIHSASLRERMPDIDIVL
                     GCGDVPARYLEFLADALDRPVYFVYGNHHEELTRKGQFGKHYEPMGAVDLGGKVCRDQ
                     GTGLIIAGFPGSPKYSRESAQQYSNNEIRWMMLKMAPRLLWNRLRRGRYVDVLISHAP
                     PRDINDRDDIPHRGFPPMRTFLRWFRPAVHLHGHIHVYDRNEPVETAYYDSTVINVYP
                     YARVELQLRPCTRAERLVDFGDNCG"
     gene            complement(781..2151)
                     /locus_tag="VFI12_07415"
     CDS             complement(781..2151)
                     /locus_tag="VFI12_07415"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HET7056276.1"
                     /translation="MATEITQQTLSINPATGAALVREYEARTPRSKELAEEARTVMPG
                     GETRTITFHPPYPLTGDHGSGCRFTDVDGNTYYDLINNYTSLLHGHANPVINEAVRTQ
                     LEKGTTFATALEAQIELGQILVERVASVDMVRYTNSGTEATMNAVRAARAHTGRELII
                     KMEGGYHGSYDDFEISVHPDLTLAGPADEPTPVIDTRGVVRSTLREVLISPLNNVDAL
                     TRLMTQYGKDVAAIIIEPVMGAAGCIPADREFLHAAQGLARQHGALFIADEVMTFRLG
                     YGGAQAYYGITPDLTTFAKIIGGGFPVGAFGGSAEVMSIFDARNPNAVYQSGTFNGNA
                     ITTVAGIAAMNLFPREEVDRLNTLGDQLRQGLAQELARHGLPGVTTGYGSMIGLHLVE
                     GEVRNYRDGAGANATFKRALHLALLLEGVFAAPRLMFCMSTPMTTETVDDVTARFGRA
                     LDRVMA"
     gene            2300..3328
                     /locus_tag="VFI12_07420"
     CDS             2300..3328
                     /locus_tag="VFI12_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HET7056277.1"
                     /translation="MIDLSSDTATKPSEGMRAAMARAEVGDEQRREDPTVNELQDMVA
                     ELTGKEAGLFLPSGTMGNVIALAVHCRRGDSVVVDKTCHIATAEGGGSAFIAQVMLKP
                     VTGERGVFTSDQVRAELTVEGTHTSPTRLISVENTTNRGGGKTWSLNALAAIRGVASE
                     AGALMHMDGARLMNAVVAGGYTAAQAASYVDSVWVDLSKGLGAPVGAVLCGTTDFIEE
                     ARLLKHRLGGAMRQAGVIAAAGIYAFEHNVERLAEDHANAKVLETGIAEIPGLEPVFG
                     PVETNIVFFDVSKTGKSAEAICDAMECAGVRMSQYPDPTKIRAVTHLDVTRADCERAI
                     EVLRSVVA"
     gene            complement(3331..>3969)
                     /locus_tag="VFI12_07425"
     CDS             complement(3331..>3969)
                     /locus_tag="VFI12_07425"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7056278.1"
                     /translation="FITPPPGYFIRVSEILQRHGIPFISDEVQAGWGRTGVADFGFQA
                     YGVEPDVVVFAKGLANGVPIGGLIATDDLASSIKSLSLSTFGGNPIATTAALANLNYI
                     ATNHLRENALAVGTYLHERLRELQTKHPVIGDVRGLGLMQAIEFVTDPMSKDPATELT
                     ARLHDEAKRRGLLVGKAGLYSNIFRLSPPLIISTSDVDDAIRILDESLIAVA"
BASE COUNT          679 a         1226 c         1352 g          712 t
ORIGIN      
        1 cggtgcggac ctgttcctca accacagttg tcaccaaaat caacgagccg ctcggcccgg
       61 gtgcacggtc ggagttggag ctcgacgcgg gcgtaggggt agacgttgat gaccgtcgag
      121 tcgtaatagg ccgtctcgac cggctcgttg cgatcatata cgtggatgtg cccatgaagg
      181 tgcacagccg gccgaaacca gcgcaggaag gtacgcatcg gcggaaaccc gcggtgcggg
      241 atgtcgtcgc ggtcgttaat atcccgaggc ggcgcgtggc tgatcaggac atcgacgtac
      301 cgccctcgcc gcagccggtt ccagaggagc cggggcgcca tcttgagcat catccagcgg
      361 atttcgttgt tgctgtactg ctgggccgac tctcggctgt acttgggcga tcccgggaac
      421 cccgcaatga tcaggcccgt gccctgatcc cggcaaacct ttccgccgag atcgacggcg
      481 cccatcggct cgtagtgctt accgaactga cccttgcgcg ttaactcctc gtggtggttg
      541 ccgtagacga agtaaaccgg tcgatcgagc gcgtcggcca ggaattcgag gtagcgcgcc
      601 gggacatctc cgcaaccgag gacgatgtcg atatcgggca tgcgttcccg cagcgacgcg
      661 ctgtgaatgc gcggatcgac ctggtccgag acggcgagca gcttgatctg gcggggcgat
      721 gggtcggtat ccgacatgcc ggaaaccata ccgcctcgtg tccagttggt gtcgaacgcc
      781 ttacgccatc acgcggtcga gcgcgcggcc gaagcgggcg gtcacatcgt cgaccgtctc
      841 cgtcgtcatc ggcgtcgaca tgcagaacat gaggcgtggc gcggcgaaga caccttcgag
      901 cagaagcgcg aggtgcaacg ctcgcttaaa cgtcgcgttg gccccggcgc cgtcccggta
      961 gttccggacc tcgccctcga cgagatgcag gccgatcatc gagccgtatc ccgtcgtcac
     1021 gccgggcaag ccatggcgcg cgagctcctg cgcaaggcct tgccggagtt ggtcgccgag
     1081 ggtgttaagg cgatcgactt cctcgcgggg gaagaggttc atggcggcga tgccggcgac
     1141 cgtcgtgatc gcgttgccgt tgaaggtgcc ggactggtag accgcgttcg ggtttcgggc
     1201 atcgaagatc gacatcacct cggcgctgcc tccgaacgcg ccgaccggga agccgccgcc
     1261 gatgatcttg gcgaaggttg tcaggtcggg cgtgatgccg taatacgctt gcgctccgcc
     1321 gtacccgagc cggaaggtca taacctcgtc cgcgatgaac agcgcgccat gttgtcgggc
     1381 cagaccctgc gcggcgtgca ggaactcgcg atcggccggg atgcagccgg ccgcgcccat
     1441 gaccggttca atgatgatcg cggcgacatc cttgccatat tgggtcatca gccgggtcag
     1501 cgcatcgaca ttgttgagcg gcgagatcag gacttcgcgg agcgtgctgc ggacgacccc
     1561 tcgcgtgtcg atgaccggcg tcggctcatc ggccgggccg gccagggtca ggtcggggtg
     1621 gacgctgatc tcgaagtcgt cgtagctgcc gtggtagccg ccctccattt tgatgatcaa
     1681 ctcgcgaccg gtgtgggcgc gagctgcacg gacggcgttc atcgtcgcct cggtacccga
     1741 gttcgtgtac cggaccatgt ccaccgaagc cacccgctcg accaggatct gcccgagctc
     1801 gatctgcgcc tcgagggcgg tcgcgaaggt cgtgcccttc tcgagctgcg tcctgaccgc
     1861 ctcgttgatg accgggttgg catgtccgtg gagcagtgac gtgtagttgt tgatcaggtc
     1921 gtagtaggtg ttgccgtcga cgtcggtgaa ccggcaaccg gagccatgat cgccggtcag
     1981 cggatagggc ggatggaacg taatcgtccg cgtctcgccg cccggcatca ccgtccgggc
     2041 ctcctcggca agctccttcg agcgcggcgt gcgcgcctcg tactcgcgga ccagggccgc
     2101 accggtcgcg gggttgatcg aaagcgtctg ctgggtgatc tcggtggcca tcggcgttga
     2161 ttctccggtc gaaatcggtt gtgccgcgtg tggcgtgatc ctatcactcc cggtgcagac
     2221 gggaccggcc tccataattg atcgtcgccg gatagaatcg cgcgggagtg ctgatgacac
     2281 gcgagattgg attgtgcacg tgattgacct gtcgagtgat accgctacga agccgtccga
     2341 gggcatgcgc gctgccatgg ctcgcgccga ggtcggcgac gagcagcgcc gcgaggatcc
     2401 gaccgtcaat gagctccagg acatggtcgc ggaactgacc ggcaaggagg cgggcctgtt
     2461 cctgccgtcc gggacgatgg gcaacgtgat cgcgctcgcg gtccactgcc ggcggggaga
     2521 ctcggtcgtc gtcgacaaga cctgccatat cgcgacggcc gagggcggtg ggtcggcatt
     2581 catcgcccag gtcatgctca agccggttac cggcgagcgc ggcgtgttca cgtcagatca
     2641 ggtacgtgct gaattgaccg tcgaaggcac gcatacgtcg ccgactcgcc tgatctcggt
     2701 cgagaacacg acgaaccggg gtggcggcaa gacctggtcg ctcaatgcgc tggctgctat
     2761 ccgcggggtc gcgagcgagg ccggggcgct gatgcacatg gacggcgccc ggttgatgaa
     2821 tgcggtcgtc gccggcgggt acacggctgc ccaagctgct tcttacgtcg actcggtatg
     2881 ggtggacctg tccaaggggc tcggcgcccc ggtcggggcc gtcctctgcg gcacgacgga
     2941 cttcatcgaa gaggctcgcc tgctcaagca ccgcctcgga ggcgcgatgc ggcaggccgg
     3001 cgtgatcgcc gcggccggca tttacgcctt cgagcacaat gtcgagcggt tggccgagga
     3061 tcatgcgaac gcgaaggtac tcgagaccgg catcgccgaa atcccgggac tagagccggt
     3121 tttcggtcca gtcgaaacca atatcgtctt cttcgacgtc agcaagaccg gcaaatcggc
     3181 tgaagcgatc tgcgacgcga tggagtgcgc aggcgtgcgg atgtcgcagt acccggaccc
     3241 gaccaagatc cgcgcagtta cgcacctcga tgttacgcgc gccgattgcg agcgggcgat
     3301 cgaggttctg cgctcggtcg ttgcctgacg tcacgcgacg gcgatcaatg actcgtcgag
     3361 gatgcggatt gcgtcgtcga catcgctggt cgagatgatc agcggcggac taagccgaaa
     3421 gatattcgag tagagcccgg ccttgccgac gagtaaaccg cgccgcttgg cctcgtcatg
     3481 cagccgcgca gtcaattcgg tggcggggtc ctttgacatc gggtcggtca caaactcgat
     3541 cgcctgcatc agcccgagcc cgcgaacgtc gccgatgacc gggtgcttcg tctggagctc
     3601 ccgcagccgc tcgtgcaggt aggtcccgac ggcgagcgca ttctcccgca ggtggttggt
     3661 cgcgatgtaa ttcaggttcg cgagcgcggc ggtcgtcgcg atcgggttgc cgccgaaggt
     3721 cgacaagctg agcgacttga tcgacgaagc cagatcgtca gttgcgatca ggccgccgat
     3781 cgggacgcca ttcgccagcc ccttcgcgaa gacgacgacg tccggctcca cgccgtacgc
     3841 ctggaagccg aaatcggcca cgccggtccg accccatccc gcctggacct catcggagat
     3901 gaacgggatg ccgtgtcgct gcaggatttc cgagacgcgg atgaagtagc ccggaggcgg
     3961 agtgatgaa
//
LOCUS       DASSGX010000420         3710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_725866, whole genome shotgun sequence.
ACCESSION   DASSGX010000420 DASSGX010000000
VERSION     DASSGX010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725866"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VFI12_07430"
     CDS             <1..485
                     /locus_tag="VFI12_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056279.1"
                     /translation="HAPTAYFAISPIIGAFIGIVLQPRWGRFGDRAGACRILLLSGVG
                     LSIVPLLWAIAPVYWFGWVVDSVAFCFWPGHTLGLTLRAVELVDNEGDRPMMLGWTNL
                     AQGTGATLSPLIASMTISELSLPVILLMAFALRLASSWVISGAVPRISQGPVPTPSTA
                     "
     gene            complement(482..781)
                     /locus_tag="VFI12_07435"
     CDS             complement(482..781)
                     /locus_tag="VFI12_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056280.1"
                     /translation="MPSENGHQEATPQRAPNSDSGGKSQSATRKFVFTAWGDAVDRDL
                     CCHALTEPPEQTPISGRTGLATAEWVVVDRRDGRALICNRLHDGRCDWPWSVETD"
     gene            916..1920
                     /locus_tag="VFI12_07440"
     CDS             916..1920
                     /locus_tag="VFI12_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HET7056281.1"
                     /translation="MTKQIGIDLGTANVLVFVRGRGIVINEPSVVAISARDGRVKAVG
                     LEARNMLGREPRETIEVVRPMRDGVIADYVVTQEMLRYFIGKAMGRFSLIRPEVMICV
                     PAGVTGVERRAVRDAALNAGARRAFLISEPLAAAIGAQIPVADPSGNMVIDIGGGTTE
                     VAVISLNGIVVARSLRVGGNKFDEAISNYIKRKYNLRVGDRTSEDIKIQIGSAMPLEE
                     DEWMEVRGRDEVAGLPRTIQIHSGEVTDAITEPLEAIIGAVRAVLEDTPPELSSDIID
                     KGMILTGGGAMLRHLPDLLTEVTGVPCYVADDPLSCVAVGTGLALEHMDILRDSLEEP
                     "
     gene            1929..3512
                     /gene="malQ"
                     /locus_tag="VFI12_07445"
     CDS             1929..3512
                     /gene="malQ"
                     /locus_tag="VFI12_07445"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HET7056282.1"
                     /translation="MEPELLSRGSGILLHPTSLPGPDGIGDLGDGAYRFVDWLALAKQ
                     RYWQVMPLVPTGFGDSPYAAPSAFAGNPLLISLDILIGDGLLDPHAFRNTPFTEGVVN
                     FSGARTQKERALRAAFGRFTERDGNGLTQEFKAFRKAEASWLDDFCLFTALKAEQGGA
                     AWSSWPDDVRRRTPEAMKAAKDRLASEIRYHAFCQWLFRRQWVALRIYANERGIRIIG
                     DIPIFVAFDSADVWANPSQFRLNEDLNPVVVAGVPPDYFSRDGQRWGNPIYDWSAMAA
                     DGYAWWVKRFRALLALVDVVRIDHFRGFAANWSVPADAPTAAEGRWERGPGRALFDAV
                     FAQLGEVPIIVEDLGLITPDVVELRKEIGLPGMAVLQFAFDGNPHNAYLPHEGERNSV
                     LYTGTHDNQTTIGWFASVPENVRTEVLAYLGRDGSDIAWDFIRTALNSQSMLAVIPLQ
                     DVLRRGDEARMNTPGRPEGNWTWRMRWTDLDSGLAEGLGLLTYLSGRATPDTPPAGFD
                     PYDYTHPGTAHPLHDSGVI"
BASE COUNT          643 a         1172 c         1194 g          701 t
ORIGIN      
        1 tgcatgcgcc gacggcctat ttcgcgatct cgcccatcat cggcgcattc attgggatcg
       61 tgttgcaacc gcgctggggc cgtttcggcg atcgagcggg cgcctgccgg attcttctgc
      121 tgagcggcgt gggattgtca atcgtgccgc tgctgtgggc gatcgcgccg gtctactggt
      181 tcggctgggt cgtcgactct gttgcgttct gcttctggcc cggccacacg ctcggtctca
      241 cgctgcgtgc ggtcgagctc gtcgataacg agggagaccg gccgatgatg ctcggttgga
      301 ccaacctggc gcaggggacc ggggcgaccc tttcgccgct gatcgcgtcg atgacgatca
      361 gcgagctgtc cttgcctgtg atcctgttga tggcgtttgc gctgcggttg gcaagctcct
      421 gggtcatctc cggagcggta ccgcggatct cacaagggcc ggtcccgaca ccgagcacgg
      481 cctagtcggt ttcaaccgac catggccagt cgcacctgcc atcgtgcagc cggttgcaga
      541 tgagcgcccg gccgtctcgc cgatcgacca caacccattc ggctgtggcc aggccggtcc
      601 gccccgaaat cggcgtctgc tcaggcggct ctgtcaatgc gtggcagcag aggtcccgat
      661 cgacggcatc gccccatgcg gtgaaaacga acttgcgggt ggcggactgg ctcttcccgc
      721 cagagtcgga gttcggtgcc cgctggggtg tcgcttcttg gtggccgttc tcgctgggca
      781 cgctattcct gccattctgc aactgcctga aagtgaccct atagtacact tggctcctgc
      841 aaacagggca gagccgccct attcgacccg cagcgctcgc ccgcgccacc gtactggccg
      901 gcggagattc tacctgtgac gaaacagata ggcattgatt tagggacggc aaacgttctc
      961 gttttcgttc gtggccgcgg catcgtgatc aacgagccgt ctgtcgtcgc catctcggcc
     1021 cgggacggca gggtcaaggc ggtcggactc gaagcacgga acatgctcgg tcgcgagcca
     1081 cgcgagacca tcgaagtcgt ccgtccaatg cgagacggcg tcatcgccga ctatgtcgtg
     1141 acgcaggaga tgctccgcta cttcatcggc aaggcgatgg gccggttctc gctgattcgc
     1201 cccgaagtca tgatctgcgt gccggctggt gtgaccgggg tcgagcgccg cgccgttcgt
     1261 gatgccgccc tgaatgcggg tgctcgccgc gcctttctga tcagtgagcc acttgccgcc
     1321 gcgatcggcg cccagatccc ggtcgccgat ccatcgggca acatggtgat cgacatcgga
     1381 ggtggcacga ccgaggtcgc cgtcatatcg ctgaacggca tcgtggtcgc gcgctctctg
     1441 cgcgtcggtg gcaacaagtt cgacgaagcc atcagcaatt acatcaagcg caagtacaac
     1501 cttcgggtcg gcgatcgcac ctccgaagac atcaagatcc agatcggcag cgccatgccg
     1561 ctcgaggaag acgagtggat ggaggttcgc ggtcgcgacg aagtggccgg actgccgcgc
     1621 acgatccaga tccattccgg cgaggtgaca gacgcgatca cggaaccgct cgaggcgatc
     1681 atcggcgcgg tccgggcggt gcttgaagac accccgccgg agctctcatc cgacatcatt
     1741 gacaagggca tgatcctgac ggggggaggg gcaatgctgc gccacctgcc ggatctgctc
     1801 accgaagtaa caggcgtccc gtgctacgtc gccgacgacc cgctctcctg cgtggcggtc
     1861 gggaccggat tggcgcttga gcacatggat attctccgcg acagcctgga ggagccatag
     1921 cgcacgtgat ggaaccggaa ctgctgagcc ggggcagcgg cattcttctt catccgacct
     1981 cgctgccagg accagacggg atcggcgatc tcggagacgg ggcctaccgc tttgtcgact
     2041 ggctcgcgct cgccaagcag cgctattggc aagtcatgcc gctcgtgccg accggcttcg
     2101 gcgatagccc gtatgccgcg ccatcggcgt tcgccgggaa cccactcctg atttcactcg
     2161 acatcctgat tggcgatggc ttgcttgatc cgcacgcgtt tcgcaacacg cccttcacgg
     2221 aaggcgtggt caacttcagc ggagcccgaa cccagaagga acgggcgctt cgcgccgcgt
     2281 tcgggcgatt cacagagcgg gacgggaacg gcctgacgca ggagttcaag gcgttcagga
     2341 aggctgaagc atcgtggctg gacgatttct gcctcttcac tgcgctgaaa gccgagcagg
     2401 gcggcgcggc gtggtcatcc tggccggacg atgtcaggcg acgcactcca gaggcgatga
     2461 aggcggccaa ggatcgactg gcctccgaga ttcgctacca cgcattttgc cagtggctgt
     2521 tccgccgcca gtgggtcgcc ctgcgaatct atgcgaacga gcgcggcatc cgcatcattg
     2581 gcgatatccc gatcttcgtc gcatttgaca gcgccgacgt ctgggcgaac ccaagccagt
     2641 tccggctgaa cgaagatttg aacccggtgg tcgtggccgg cgtgccgccg gattacttct
     2701 cgcgggatgg gcagcgctgg ggaaacccga tttacgactg gtcggcgatg gcggcggacg
     2761 gctacgcatg gtgggtcaag cggttccggg cgctgttggc gctggtcgac gtcgtgcgga
     2821 tcgaccattt ccgcggtttc gcggccaact ggtcggtccc ggccgatgca ccaactgctg
     2881 ccgaagggcg atgggagcgg ggaccgggcc gcgccctgtt tgacgcggtc ttcgcgcaac
     2941 tcggcgaggt gccgatcatc gttgaggatc tgggtctgat cacgccggat gtcgtcgagc
     3001 tccgaaaaga gatcggttta cccggcatgg cagtcctgca gttcgccttc gacggcaacc
     3061 cgcacaacgc ctatttgccg catgaaggcg agcggaactc cgtgctctac accggcacgc
     3121 atgacaacca gacgaccatc ggttggttcg cgtcggtccc ggagaatgtc cgcactgagg
     3181 ttttggccta tctcggccgc gacggtagcg acatcgcctg ggacttcatt cgcaccgcac
     3241 taaactccca gtccatgctg gctgtcatcc cgctgcaaga cgtgcttcgg cggggagacg
     3301 aggcccggat gaacacaccc ggccggccgg aagggaactg gacctggcgg atgcgctgga
     3361 ctgacctgga ttccgggttg gccgaagggt tgggtttgtt gacatatctc tctggacgag
     3421 ccacgccgga tactccgccg gcaggattcg acccctatga ctacacccat cccggaacgg
     3481 cccatccatt gcacgattcc ggtgtcattt agacttgcaa acgacgcctc cgatctgact
     3541 cgcgacgaaa ggacacctgc cgaatggcga aacgcggagc cttcaccttt gtcctccata
     3601 gccacctgcc gtacgcgcgg aatgccggca tgtggccgca cggcgaagag tgggtccacg
     3661 aagcgatcgc cgacacgtac atcccgatcc tcgatgcgct gaacgagctc
//
LOCUS       DASSGX010000421         2444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_180492, whole genome shotgun sequence.
ACCESSION   DASSGX010000421 DASSGX010000000
VERSION     DASSGX010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180492"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VFI12_07450"
     CDS             <1..541
                     /locus_tag="VFI12_07450"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET7056283.1"
                     /translation="IAADDRTPTTELQPRVEDILRDTRRITPDRDPDFSIRDMTQIAD
                     AMTSATTTMTGMLGAVAGVSLLVGGIGIMNIMLVSVTERTREIGIRLAIGARESQVLS
                     QFLIEAIALSMFGGVAGIVLGLLVSFVATRALGVPFIVDWTSVLLAFGFSAFVGVAFG
                     YFPARGAARLDPIQALRHE"
     gene            complement(657..1412)
                     /locus_tag="VFI12_07455"
     CDS             complement(657..1412)
                     /locus_tag="VFI12_07455"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HET7056284.1"
                     /translation="MSSDTSSRTLPRPKAKIDPQQTPFVGNETVEDNLHEAVEVAIWT
                     VLGQGCSPVVVEVRDGGVVALTGTLPSETHRRDVVAAVRALKGVMSVVDHLDIGADET
                     VATPSAKAGDMLYVRRFCAADETSTSAAIRQAIGRLATHFAEQKMAPTTLVVIYRNLR
                     PQTVTLDIAMPVPAGTTMPNAGTLRVARMPASNAFETVAQPGFDGLIEAVTTLKKKAG
                     IRRNDTAAFWQSFDLDQVRPWTGHPQAKVHLAA"
     gene            complement(1430..2080)
                     /locus_tag="VFI12_07460"
     CDS             complement(1430..2080)
                     /locus_tag="VFI12_07460"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7056285.1"
                     /translation="MLTQTTFPIAAISGSSQDPDVRGLLTQSQPGAMTFYPVDATIYA
                     QGDEAGPLYLVEFGTVRICQMTADGRRQITSFCYAGDVFGLEPGSEHEFYAESVDGAG
                     IRVLRPANAVGFAQKLLSIALRRFARMQSHLVLLGRLSANEKIASFLCDLLDRQDSDD
                     VVHLPMQRADIADYLGLTFETVSRVLRVLKDRGIVRVDSVDRIDVVDYDALRSLTD"
     gene            complement(2159..>2444)
                     /locus_tag="VFI12_07465"
     CDS             complement(2159..>2444)
                     /locus_tag="VFI12_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056286.1"
                     /translation="FALQAEGYDVTSCASIGARERPRYDCTIVDHHALGNNQRFAADF
                     CRIFRPVILLANLVPHPLSPFAYRTVMKPLLGAALTDAIHDAIGHRAATT"
BASE COUNT          437 a          745 c          826 g          436 t
ORIGIN      
        1 catcgccgcc gatgacagga cacccaccac cgaactgcag ccgcgcgtcg aggacatctt
       61 gcgcgatacc cgccgcatca cgccggaccg cgatcccgat ttctcgatcc gcgacatgac
      121 ccagatcgcc gacgccatga ccagcgccac caccaccatg accggcatgc tgggcgccgt
      181 cgccggcgtc agcctgctgg tcggcggcat cggcatcatg aacatcatgc tggtctcggt
      241 caccgagcgg acacgagaga tcgggatccg tctcgcgatc ggcgcgcgcg agtcgcaagt
      301 gctttcgcag tttctaatcg aagcgatcgc cttgtcgatg ttcggcggcg tggccggcat
      361 cgtgcttggg ttgctggtgt cgtttgtggc gacacgagcc ctcggggtgc cgttcatcgt
      421 cgactggacc tcagttctcc tggccttcgg gttctcggcc ttcgtggggg ttgcgttcgg
      481 ttacttcccg gcgcgcggtg cggcacggct cgatccgata caggcgctac ggcacgaatg
      541 aggttggccg cccgccgggc ggcgccggcg acatcgcagg cgaaacgtca ttggcaagcg
      601 aacgttcgag ctgggcacca gctcgaacgt cggacctttc cagaggaact gccggcttag
      661 gcggccaggt ggaccttggc ctgagggtgt ccggtccagg ggcggacctg atcgaggtcg
      721 aacgactgcc agaatgcggc ggtgtcgttc cgacggatgc cggccttctt cttgagcgtc
      781 gtcacagcct cgatcaggcc gtcgaaacca ggttgggcga ccgtctcgaa ggcattggaa
      841 gcaggcattc gggcgacacg caaagtgccg gcatttggca ttgtcgtgcc cgcggggacg
      901 ggcatcgcga tgtcgagcgt gaccgtctgc ggccgaagat tgcggtagat gacgaccagc
      961 gtggtggggg ccatcttctg ctccgcgaag tgcgtcgcga gccggccgat ggcctggcgg
     1021 atcgcggcgg aagtcgaggt ttcgtcggcg gcgcagaagc gacggacata aagcatgtcg
     1081 ccggctttcg ccgacggtgt ggccaccgtc tcgtccgcac cgatgtcgag gtgatccaca
     1141 acggacatga cacctttgag cgcgcggact gcggcaacaa cgtcgcgcct gtgtgtttcc
     1201 gagggcaggg tcccggtgag ggcaaccacg cccccgtccc gcacctcgac caccacaggg
     1261 gagcaacctt gccccagaac cgtccagatc gccacctcga ccgcttcgtg gaggttgtcc
     1321 tcgacggtct cgttgccgac gaagggcgtc tgctgcgggt cgatcttggc cttgggcctc
     1381 gggagggtcc gggatgatgt atccgaagac atctggaact ccattgtcat caatccgtca
     1441 ggctgcgaag ggcgtcgtag tccacgacgt cgatgcggtc gaccgagtct acgcggacga
     1501 tgcccctatc cttgagcacg cgcagcacgc gcgaaacggt ctcgaaggtc aacccgagat
     1561 agtcggcgat atcggcacgc tgcatcggaa ggtggacgac atcgtcggaa tcctgacggt
     1621 caagcagatc gcacaggaag ctcgctatct tctcattggc gctgaggcgg ccaaggagca
     1681 ccagatgcga ctgcatgcgg gcgaagcgcc gcagggcgat actgagcagc ttctgcgcaa
     1741 agcccaccgc attggcgggg cgaagcacgc gaatgccggc gccgtcgacg ctctcggcat
     1801 agaattcgtg ctcactgccc ggctccaggc cgaacacatc tccagcgtag cagaaactcg
     1861 tgatctggcg acggccgtcg gcggtcatct ggcagatgcg gacggttcca aattcgacga
     1921 gatacagcgg accggcttca tcaccctgtg cgtaaatggt ggcgtcgacc ggatagaagg
     1981 tcatggcacc cggctggctc tgggtgagca gtccgcggac gtcggggtcc tgtgacgagc
     2041 ccgagatcgc cgcgattgga aaggtcgttt gcgtgagcat tctgtgtacc tcgttgaagt
     2101 gaggcgacac tgctgcagat cgtccgccgg cgaaattccg gtcgggggac tagggggact
     2161 acgtagtggc ggcccggtgc ccgatcgcgt catggatggc gtcggtcaga gccgcgccga
     2221 gcagcggctt catcaccgta cgataagcga agggggacag ggggtgcggc acgaggttgg
     2281 cgagcaggat aaccggcctg aatattctgc agaaatcagc tgcaaaacgc tggttgttac
     2341 ccagggcatg atggtccacg atcgtgcagt cgtagcgtgg ccgctcccgg gcgccgatgc
     2401 tcgcgcagga ggtgacgtcg tagccctccg cctggagtgc aaat
//
LOCUS       DASSGX010000422         5881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1598482, whole genome shotgun sequence.
ACCESSION   DASSGX010000422 DASSGX010000000
VERSION     DASSGX010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5881
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1598482"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..1599)
                     /locus_tag="VFI12_07470"
     CDS             complement(163..1599)
                     /locus_tag="VFI12_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056287.1"
                     /translation="MSRSQYTVSRRGVLAGSLASIGLANRAGRVRGQTPVSRESKSFG
                     ILVGTGDLTDSVALFDSATGDVVYRYDIGPRPNAAWATPVQGLALVRCERSLAIADLT
                     DGTMRPIAVPETIAPSLSPAGIQFRGSAGYGRILVGTPNSDANTFLVDLMTGERRSVV
                     GMLGAQKPPVSLQNVAVASDDRHLLAWDGRTTWIVDIATGASRVLGSGQFTFSAEFSG
                     DGSHLVYSQQLASGTTQLRMQAVDGSGDELLAESAFILVSLWLPTRDMMLIDERNDEG
                     GYLAIFDPATGVRGDLLAYTGATNIVQFSPDGRHALVGIEGDQGRDWYKLQLSLPGGY
                     SRLLDGLTDAVILPGFAFASAWALALPPTATGTESGTIKAVDLAAGTISPLVTGITSD
                     AELTGELVAPSGNAALLTIDSFTELAVHYLRLDKPGDVAVDLIAPGPSVIAPDGAGFA
                     VNQNDMTVVHDETGAEGATFPGNALAWV"
     gene            1711..2487
                     /locus_tag="VFI12_07475"
     CDS             1711..2487
                     /locus_tag="VFI12_07475"
                     /inference="COORDINATES: protein motif:HMM:TIGR03856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HET7056288.1"
                     /translation="MTIRPIRIGISARPQHTTVERMRETWRTAEEFGVDDIYIWDHFF
                     PLHGDPHGAHFECWTLLAALAEVTSRVRIGPLVSAIGYRNPNLIADMARNIDHLSGGR
                     FILGLGSGWKEEDYAEYGYDFMTARDRLRDLEAKLPVIKDRLAKLNPPPVQQPLPILI
                     GGSGEKVTLRIVAEYADMWNGFGDPKEAGRLSRVLDDWCAKVGRDPAAIERSIMPAAK
                     DAVANADAYVAQGITTLIVGVDGPEQLAVVRDLVAWRDSR"
     gene            2564..2746
                     /locus_tag="VFI12_07480"
     CDS             2564..2746
                     /locus_tag="VFI12_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056289.1"
                     /translation="MALQITERNGRKYVTATNARVNFGALLDYIKATGATVIVTVRGK
                     PRVQIRPKYPNESAVT"
     gene            2743..3051
                     /locus_tag="VFI12_07485"
     CDS             2743..3051
                     /locus_tag="VFI12_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056290.1"
                     /translation="MNRETDTKTRRIAATEARVHFGELVDDVVAGRKIAEVDRADGYT
                     VVVEPADDEADKPGFDAKAWFANLRRIQAEIRAWRERNGIPEITEMPEDIMRELHESR
                     "
     gene            3051..3476
                     /locus_tag="VFI12_07490"
     CDS             3051..3476
                     /locus_tag="VFI12_07490"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HET7056291.1"
                     /translation="MPSDLICADNSIWIRIMVDIDRRLAIEASGWFDAGVPMLAPGLL
                     HYEFSHVLSRYTRNRRLSPTYANALLESLLALEIELIDDNDLHIQALKHSTAHPGLSG
                     YDAHYLAVCERTGAELWTADKRLATFAEASDIAARLWVT"
     gene            3708..4967
                     /locus_tag="VFI12_07495"
     CDS             3708..4967
                     /locus_tag="VFI12_07495"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HET7056292.1"
                     /translation="MAADTASSDKQKLGKVVLAYSGGLDTSAIIPWLKENYDCEVIAF
                     AANLGQPDDLSNLEQKALASGASKLHLRDLREEFLTEYAFPTLRAGAIYERKYLLGTS
                     MARPLTAKHQVEVALLEGADAVAHGCTGKGNDQVRFELTYQALGPHLQIIAPWRHWHI
                     RSREDAIDYCEAHGVPVTASKTKIYSVDGNLWHLSHEGGLIEDPWNEPPADAYDLTVA
                     PEDAPDVPEYVTVGFEQGWPVSINGQKLSPLEIMAALNEIGAKHGVGRVDLLENRLVG
                     MKSRGIYETPGGTILSNAHRELEHLVIDRMTLRQKDQLAQVYADLVYNGQWFSPLKGA
                     LDAFVDKTQEVMTGEVKVKLYKGQAIAVARKSPYSLFREDLATFGKDDVYRQADAEGF
                     IRLFGLPTKVAAMRDRAVRESNAEVCG"
     gene            5382..>5881
                     /locus_tag="VFI12_07500"
     CDS             5382..>5881
                     /locus_tag="VFI12_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056293.1"
                     /translation="MTDRLTGTDGAKTTPSYLRPVTSGYSLPADNEERPMLSLGGSQA
                     VTLKTFTISAGMRGRSLVDDTDFTPDELAEILDTAARLKRMHRRGEAHSYLAGKTLAM
                     IFQHPSTRTRVSFEAGMTQLGGHALYLGMQDLQLRRGETVSDTAKVISRYCDVILARV
                     VSHDDIA"
BASE COUNT         1144 a         1893 c         1864 g          980 t
ORIGIN      
        1 cccacggtcc gcgcaggtcg acccactgat cccgccgcaa ctgggggcga ggatagtcgg
       61 tcgagtcgga aggtatcgac ataacggagt cctcgtgcca atgaacgtct gctcgcattg
      121 ctctcggcac tctaccgaaa cgattgcctc gttgcggtgc gatcagaccc aggccagggc
      181 attaccgggg aacgtcgctc cttcggcgcc ggtttcgtcg tggacgacgg tcatgtcgtt
      241 ctggttgacg gcgaatccag ctccatccgg cgcgatcacg ctcggtccgg gggcgatcag
      301 gtcgaccgcg acgtcacccg gtttatccag cctcaggtag tgcaccgcca gttcggtgaa
      361 actgtcaatt gtgagcaggg cggcatttcc gctcggcgca accagttcgc ccgtcaactc
      421 ggcgtcgctc gtgatccctg tgaccagtgg cgagattgtc cccgccgcaa ggtcgacggc
      481 cttgatcgtc ccgctctcgg tcccggttgc ggtcgggggg agtgcgagcg cccatgctga
      541 agcgaaagca aacccgggga gaatgaccgc atcggtcaaa ccgtcgagca ggcgtgagta
      601 gccgcccggc agcgaaagct gaagcttgta ccagtcgcgg ccctggtcac cttcgatccc
      661 gaccagcgca tgtcgtccgt ccggcgagaa ctggacgata ttcgtcgcgc ccgtgtatgc
      721 gagcaggtca ccgcgaacgc cggtggccgg gtcgaagatc gcgaggtatc cgccctcatc
      781 gttccgctcg tcaatcagca tcatgtcgcg ggttgggagc cagaggctaa cgagaatgaa
      841 cgcactctcg gccaggagct catcgcccga gccatcgacg gcctgcatcc ggagctgggt
      901 ggtgccggat gcgagctgct gcgagtagac gaggtgggaa ccatccccgc taaactcggc
      961 cgaaaaggtg aactggcccg aacccaggac gcgactggcg cccgtggcga tgtcgacgat
     1021 ccaggtcgtc cggccgtccc acgccagcaa atggcgatcg tcggacgcga cggccacgtt
     1081 ctggagcgag accggcggtt tctgtgcccc gagcatgccg accactgacc ggcgctcccc
     1141 ggtcatcagg tcgaccagaa acgtgttcgc atcggaattc ggcgtgccga ccaggattcg
     1201 cccgtacccc gcgcttccac ggaactggat cccggccggc gagaggctcg gcgcgatcgt
     1261 ctcggggacc gcgatcggtc gcattgtgcc gtcggtcaaa tcggcgatcg ccagcgagcg
     1321 ttcacagcgg accagcgcga gaccctggac cggcgtcgcc caggcggcgt tcggacgcgg
     1381 gccgatatcg taccgataga ccacgtctcc ggtggcggaa tcaaagagcg cgaccgaatc
     1441 ggtgaggtcg cctgtcccga ccaggattcc gaacgacttc gattcgcgtg aaaccggcgt
     1501 ctggccccga actcgaccgg cacgattggc cagcccaatc gatgcaagcg aaccggccag
     1561 cacgcctcgc cgggaaactg tgtattgcga acgcgacatc aatctctccc ccgctgccag
     1621 tctaccctct gcaaacgggc agtcgttcgc cggcgcccgg cgtcgtagag tgccgaacgg
     1681 aactgcatcg gattgaatcg ggagaactga atgacaattc gtccaattcg catcggaatt
     1741 tcggcccggc cacagcacac caccgtcgaa cggatgcggg agacctggcg gacggccgaa
     1801 gagttcggcg tcgacgatat ctacatctgg gaccacttct tcccgctcca cggcgacccg
     1861 cacggcgcgc acttcgaatg ctggacgctg ctcgcggcgc tggccgaggt gacatcgcgc
     1921 gtccggatcg gaccgctcgt ctccgcgatc gggtatcgca acccgaacct gatcgccgac
     1981 atggcccgga acatcgatca cctctcgggc ggtcgcttca tcctgggact cggttccggc
     2041 tggaaggaag aggactacgc cgagtacggc tacgacttca tgaccgcccg ggaccgcctg
     2101 cgcgacctcg aggccaagct gccggtcatc aaggatcggc ttgccaagct caacccgccg
     2161 ccggtccagc agccgctgcc gatcctgatc ggcggttcgg gcgagaaggt cacgctccga
     2221 atcgtcgccg agtacgccga catgtggaac ggcttcggcg acccgaagga ggccggacgg
     2281 ctgagccgcg tgctcgacga ctggtgcgcg aaggttgggc gcgatccggc cgcgatcgag
     2341 cgctcgatca tgcccgccgc gaaggacgcg gtcgccaatg ccgacgccta cgtggcgcaa
     2401 gggatcacga ccctgattgt cggcgtcgat ggaccggagc agttggcggt agtccgggac
     2461 ctcgtcgctt ggcgcgacag ccgataaggt gcttgccctg actatgatga cgtcctatga
     2521 tgcccttgtt gattgatcaa tcaatcaacg aggagattcc actgtggccc ttcaaattac
     2581 tgagcggaac ggccgcaagt acgtgaccgc gaccaacgcg cgggtcaact tcggcgcgct
     2641 gcttgattac atcaaagcga ctggcgccac ggtcatcgtc accgtgcgcg ggaaaccgcg
     2701 cgtgcaaatc aggcccaagt acccgaacga gagtgcggtc acatgaatcg agagactgat
     2761 acgaaaacca gacgaatcgc agcgaccgag gctcgcgttc acttcggcga gctcgtcgat
     2821 gacgtggtgg ctggcaggaa gatcgccgag gtggatcgcg cggacggata caccgtcgtc
     2881 gtcgaaccag ctgatgatga ggcagacaag ccaggattcg acgccaaagc ctggttcgcc
     2941 aaccttcgcc gtatccaggc ggaaatccgg gcatggcgtg agcggaatgg cataccggaa
     3001 atcaccgaaa tgcctgagga catcatgcgg gagttacatg agagccgctg atgcccagtg
     3061 acctgatttg cgcggataac agcatctgga tcaggatcat ggttgatatt gatcggaggc
     3121 tggctataga agcatcagga tggttcgacg ccggcgttcc gatgcttgca cccggccttc
     3181 ttcactatga gttctcgcac gttctaagcc ggtacacccg aaaccgaagg ttgtcgccga
     3241 cgtatgcgaa tgcattgctt gaatcgcttc tggcactgga aatcgaactc atcgacgata
     3301 atgaccttca cattcaggca ctcaagcatt cgacggccca tcccggactt tccggatacg
     3361 acgcccatta cctggcggtt tgcgagcgca cgggagccga gctttggacg gcagacaagc
     3421 gcctggcaac gttcgctgag gcaagcgaca tcgcggcgcg cctctgggtt acttgacaag
     3481 atggcgcggg gttcggtacc ttgtgcccgt cgcggacgac attggctcgt ccgttttttg
     3541 attgccacga tgcaggactt tacgctcgtg caggacctcc gattcaagcc aacgccgccg
     3601 cgtcgcccgc gtagccgccg gacgggtttc cgttcggcgt aagcaggatc ccatcgtcca
     3661 cggctggcga tggatggcgt tgacagattg aatccggaga caaacccgtg gcagcagaca
     3721 ccgcctcctc cgacaagcag aaactcggca aggtcgttct cgcctattcc ggcggcctcg
     3781 acacgtcggc catcatcccg tggctgaagg agaactacga ctgcgaggtc atcgcctttg
     3841 ccgcgaacct cggccagccc gatgacctca gcaatctcga gcaaaaggcg ctcgccagcg
     3901 gggccagcaa gctccacctg cgggacctgc gcgaggagtt cctgactgaa tacgccttcc
     3961 cgacgctccg cgccggcgcg atctacgagc ggaagtattt gctcggcacc tcgatggccc
     4021 ggccgctgac ggccaagcac caggttgagg ttgccctgct cgaaggggcc gacgcggtcg
     4081 cccacggctg caccggcaag ggcaacgacc aggtccgctt cgagctgacc taccaggcgc
     4141 tcggcccgca cctccagatt atcgccccgt ggcggcattg gcatatccgc tcccgcgagg
     4201 acgcgatcga ctattgcgag gcgcacggcg tcccggtcac ggccagcaag acgaagatct
     4261 actcggtcga tggcaacctg tggcacctgt cccacgaggg cggtctgatc gaggatccat
     4321 ggaacgagcc gccggcggat gcctacgacc tcaccgtcgc gcccgaggat gcgccggacg
     4381 ttcccgagta cgtcacggtc ggattcgagc aggggtggcc ggtgtcgatc aacggccaga
     4441 agctctcgcc gctcgagatc atggccgcgc tgaatgagat cggtgcgaag cacggggtcg
     4501 gccgggtcga cctgctcgag aaccggctgg ttggcatgaa gagccgcggc atttacgaaa
     4561 cgccgggcgg cacgatcctg tcgaatgcgc accgggagct cgagcacctg gtcatcgacc
     4621 ggatgacgct gcgtcagaag gaccagctgg ctcaggtcta cgcggacctc gtctacaacg
     4681 gccaatggtt ctcgccgctc aagggcgcgc tcgacgcctt cgtcgacaag acgcaggagg
     4741 tcatgaccgg cgaagtcaag gtcaaactct acaagggcca ggccatcgcg gttgcgcgga
     4801 agtcgccgta tagcctcttc cgggaggacc tggccacatt cggcaaggac gacgtctacc
     4861 ggcaggccga cgccgagggc ttcattcgcc tgttcggact gccaaccaag gtcgccgcga
     4921 tgcgggatcg cgccgtccgg gaaagcaacg cggaggtatg cggatgagca tcatcatcac
     4981 gggcgggaat ctctcccgct ctccgttccg gcggtcgtcg cttcgtacga agcggtagcg
     5041 accgccgacg cagacctcac aaccacagac ctgcgacgac agggaccagt agctggccga
     5101 tggccatcag cgaaccgggg atggtggaag cccggtacgg accgatcacc agcgaacgcg
     5161 cccttgagca gtcgcccgaa gaacgccggg atatcgcccg gcaagtaggg tcgagtgggt
     5221 ggctcccacg acagcaacag ccgagagacg cttctcgcct cttgagaagc aagaagggtg
     5281 gtaccgcgag cattgacgca tctcgcccct tcagccggat ctgttccggc ggcgtgcgtc
     5341 cgaccctcgt tgcgacgagt cggagcagga aggatgacga gatgacggat cgattgaccg
     5401 ggaccgacgg cgccaagacc acgccaagct atttgcggcc ggtgaccagc gggtattccc
     5461 tgcctgccga taatgaagag cgcccgatgc tgtcgctcgg agggagccag gccgtgacgc
     5521 tcaagacgtt caccatctcc gccggcatgc gcggccgcag cctggtggac gacactgatt
     5581 tcacgccgga cgagctggcc gagattctcg ataccgccgc ccgcctcaag cggatgcacc
     5641 ggcgcggcga ggcgcacagc tacctggccg gcaagaccct ggcgatgatc ttccagcacc
     5701 caagcacccg tacccgcgtc tccttcgagg ccggaatgac ccaactcggc ggccacgccc
     5761 tgtacctcgg catgcaggac ctgcagctcc ggcgcggcga gacggtcagc gacacggcga
     5821 aggtgatcag ccgctattgc gatgtcatcc tcgcccgggt tgtcagccat gacgacatcg
     5881 c
//
LOCUS       DASSGX010000423         3849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1162188, whole genome shotgun sequence.
ACCESSION   DASSGX010000423 DASSGX010000000
VERSION     DASSGX010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162188"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VFI12_07505"
     CDS             complement(<1..533)
                     /locus_tag="VFI12_07505"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET7056294.1"
                     /translation="MVLSSYAGAAVRRKEDPRLITGASTYVDDVKLPNLVEAAFVRSP
                     YAHARINGIDATDALAMPGVIAVYTAADLKKIMPSAWPDTTGETGKSSEEVAAEDDAP
                     IPVPHVYPLATDKVRYVGDPVAVVIAETRALAEDAAAMVTIDAEMLEPAIDPYEAMED
                     GAPQLYDNVKNNISVVE"
     gene            complement(725..1471)
                     /locus_tag="VFI12_07510"
     CDS             complement(725..1471)
                     /locus_tag="VFI12_07510"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7056295.1"
                     /translation="MSDTIRVLIVEREPLFRRGLAGCLATDNELQLVGSVGTADDGYR
                     TADEQLPDVTLIGTTLADGPGLNAATEFRRRFPSIAVIVVAAQETDDELFGAIRAGAS
                     AYTGQDIDETKLITLIKRAATGEYVINEQLLDKPYVAARVLEQFRSATSSDLAPHSAF
                     APLTDRELEILRKVSDGMTNAEIGYALGISAQTVKNHVTSILRKLAVNDRTQAVVTAL
                     KRGWLSIDDANPTGRNGAGVGSGAGIDHLR"
     gene            complement(1785..>3849)
                     /locus_tag="VFI12_07515"
     CDS             complement(1785..>3849)
                     /locus_tag="VFI12_07515"
                     /inference="COORDINATES: protein motif:HMM:NF019758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HET7056296.1"
                     /translation="TLDASDMLPAIYFLFSRADCQSYAERLAQMRPNLVSRAAQRRIR
                     TVINRYLDALRPEDRELEQVQLITRLARVGIGFHHAGLLPILKQLVESLFGSGLMQVV
                     FATDTLALGVNMPARTVVIGRMTKWDGRRRRPLTPNEFQQMAGRAGRRGMDAFGHVVV
                     PYSPWMAFREMLTVATGELEPVRSSFAVRYNTVLHLWDPPDGQRVRQMLRQSLAQFQA
                     SQRIRQLEHDIIEIGGDIAGTPKGCLIGLDAGDELLEDYRRVIASHTTAEQGRRRLDK
                     QLSDLQRETSATPWGEVNRQALRKLFRHAKPGVVFFHRTRGWGIFVARAEGDSGVGLT
                     IFDDTLVQSSNYRDVVFVSEHVRAQVPDDLVIDVLAGKQPSKQVLAEVSDQLADAPLP
                     DVAAQMAEHAARQRALVEDDVRRVQNELAQSTSILDALSEERDQHPCHPCPRRKEHRD
                     YLIRIERLDQERIALEELLVRETEAEEQRIQSVLWGIREVLHRFGYMRRGYATEKADM
                     LAGVFDTDGLILCELIDRGVLVDLAPEDLAEVFSWYSFDRDFRYGNQFVLPPAFALIR
                     RRIEEIERAVISEEREHSLFISEGHNPSFYGPARAWCLGKTMVEITETIQLSEGDLVL
                     TFNKTIDLVKQVREMLEDVKPEHGLIGPLRTTEKMLCRGIVEQSLNIGFAPIETEPDT
                     AVTAP"
BASE COUNT          630 a         1226 c         1289 g          704 t
ORIGIN      
        1 tgctcaacga cgctgatgtt gttcttgacg ttgtcgtaga gctgcggcgc gccgtcctcc
       61 atcgcctcgt acggatcgat cgccggctcg agcatttcgg cgtcaatggt gaccattgct
      121 gccgcgtcct cggccagggc gcgagtctcg gcgatcacga ccgcgaccgg atcgccgacg
      181 tagcggacct tgtcggtcgc cagcgggtag acgtgcggga cgggaatcgg ggcgtcatct
      241 tcggccgcga cctcctctga ggacttgcct gtttcgccgg tggtatctgg ccaggcggac
      301 ggcatgatct tcttgaggtc ggcggcggtg tagacggcga tcacgcccgg catggcgagc
      361 gcgtcagtgg cgtcgatacc gttgatcctc gcgtgggcgt acgggctgcg cacgaaggct
      421 gcctcgacca ggttcggcaa cttgacgtcg tcgacgtagg tcgaagcgcc cgtgatcagc
      481 cgggggtctt ccttgcggcg aacggccgct cctgcgtagc tgctcagaac catggcgtac
      541 tgctcgctcc tcttccacgg gaacgctccg gcattggcgc gtccatcaca cagtgcttcg
      601 cgggattgta tcagtccctc tccgggatcc ggggacggct cgttgggcga tccggcagta
      661 cacgacaaga acgggccggc agcatgccga cccgttccga ctgatctgaa attgcccgta
      721 agagctagcg caggtgatcg attccagctc cgctgccgac gccggcgccg ttgcggccgg
      781 tcgggttggc gtcgtcgatc gagagccagc cgcgcttgag ggcggtcaca acggcctgtg
      841 tgcggtcgtt gacggccagc ttgcgcagga tcgaagtgac gtggttcttg accgtctggg
      901 cgctgatgcc gagggcgtac ccaatctcgg cgttggtcat gccatcgctg accttgcgca
      961 ggatctcgag ctcgcggtcc gtcagcggcg cgaaggcgct gtgcggggcg aggtcgctcg
     1021 atgtggccga gcggaactgc tcaaggacgc gggctgcgac atacggcttg tccagcaact
     1081 gctcattgat aacgtactcg ccggtggcgg cacgcttgat cagggtaatc agcttcgtct
     1141 cgtcgatatc ctggccggta tatgccgagg cgccggcccg gatcgcgccg aagagctcat
     1201 cgtccgtctc ctgcgcggcg accacgatca cggcgatcga cgggaagcgg cgacggaatt
     1261 cggtggcggc gttcaggcca gggccatcag ccaaggtggt gccgatcagg gtgacgtccg
     1321 gaagctgctc gtctgcggtg cgatagccgt catcggctgt cccgacggaa ccgacgagct
     1381 ggagttcatt gtctgttgcc aggcagcccg ccagccctcg gcggaacagt ggctcccgct
     1441 ctacgatcag gacgcgaatg gtatcgctca ttaataatcc tcacctggaa aacgtgtcgt
     1501 gcgttgcggt tgaacgctca acgcaacgga gccggcaatg cgaggcaagt gagcgcgtag
     1561 accacgcggt ctgctctgtc accgggtcgc tgccaacctg caattccccc gcggttggcg
     1621 cgtgccaggc tcgatgtagc ccagtgtacg tttaccctgg tcatagcaca actggaccat
     1681 agtccttact cctgacccga atttcctggc tggatgggcc gcttgtcccg accgcgaacc
     1741 acggtacggc cggtcgtccg gttcattccg gccggaattt ggcgttacgg cgcggtaacc
     1801 gcggtgtcgg gctcggtctc gatcggcgcg aagccgatgt tgagcgactg ctcgacgatg
     1861 ccccggcaga gcatcttctc cgtcgtccgg agggggccga tcagcccatg ctccggcttg
     1921 acgtcctcca gcatctcccg gacctgcttg accaggtcga tcgtcttgtt gaaggtcagg
     1981 accaggtcgc cctcagagag ctggatcgtc tcggtgatct cgaccatcgt cttgccgagg
     2041 caccacgccc gggccggacc gtagaacgac ggattgtggc cctcggagat gaagaggctg
     2101 tgctcccgct cctcggagat aacggcgcgc tctatctctt cgatccgccg ccgaatcagc
     2161 gcgaacgcgg ggggcaacac gaactggttg ccgtaccgga agtcgcggtc gaagctgtac
     2221 caggagaaga cttcggccaa atcctccggc gccaggtcga ccagcacgcc ccgatcaatc
     2281 agctcgcaga ggatcagccc gtcggtgtcg aacacgccgg ccaacatgtc ggctttttcg
     2341 gtcgcgtagc cccggcgcat gtagccgaac cggtggagca cctcccggat gccccaaagg
     2401 actgactgaa tccgctgctc ctcggcctcg gtctcccgga ccagcagctc ctcgagcgca
     2461 atccgctcct ggtcgagccg ctcgatccgg atcaggtaat cgcggtgctc tttgcggcgt
     2521 ggacagggat gacacgggtg ctgatctcgc tcctcggaca aggcgtcgag gatggacgtg
     2581 ctctgggcga gctcattctg gacccggcgg acgtcatcct cgaccagggc gcgctggcgg
     2641 gcggcatgct cggccatctg ggcggccacg tccggcagcg gtgcgtcggc gagctgatcg
     2701 ctgacctctg ccagtacctg cttcgatggc tgcttgccag ccagcacgtc gatgacgagg
     2761 tcatccggaa cctgcgcgcg gacgtgctcc gagacgaaga cgacatcccg gtagttggag
     2821 gactggacga gagtatcatc gaagatcgtc aggccgacac cgctgtcccc ttccgcccgg
     2881 gcgacgaaga tgccccagcc gcgggtccga tggaagaaga cgacgccggg tttggcgtgg
     2941 cgaaacagct tgcgcagtgc ctggcgattg acctcgcccc acggtgtggc ggacgtttct
     3001 cgttgcaggt cgctgagttg cttatcgagt cggcggcggc cttgctcggc ggtggtgtgc
     3061 gatgcgatca cgcggcggta gtcctcgagc agctcgtcac cggcatcgag gccgatcagg
     3121 cagcccttcg gcgtgccggc gatgtccccg ccgatctcga tgatgtcgtg ctccagttgg
     3181 cggatgcgct ggctggcctg aaactgggcc agactctggc ggagcatctg gcgaacgcgc
     3241 tggccgtccg gcggatccca caagtgcagc accgtgttgt accggaccgc gaacgaggag
     3301 cgaaccggct ccagttcgcc ggtggcgacg gtcaacatct cgcggaatgc catccagggc
     3361 gaatagggga cgacgacgtg gccgaaagcg tccatcccgc gccgcccggc ccggcccgcc
     3421 atttgctgga actcgttcgg cgtcagcggg cgccgccgcc ggccatccca cttggtcatc
     3481 cggccgatca caacggtgcg cgccggcatg ttgacgccga gcgcgagggt gtcagtcgcg
     3541 aacacaacct gcatcagccc gctgccgaaa agcgattcga cgagctgctt caggatcggc
     3601 agcagcccgg cgtggtggaa gccgatcccg actcgcgcca gccgcgtgat cagttggacc
     3661 tgctccaact cgcgatcctc gggccgcagg gcgtcgagat agcggttgat gacggtccgg
     3721 atccggcgct gcgcagcccg gctgacaagg ttcgggcgca tctgggcaag tcgctcggca
     3781 tacgactggc aatcggcccg gctgaacagg aaatagatcg ccgggagcat gtcgctggca
     3841 tcgagggtg
//
LOCUS       DASSGX010000424         2104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1162770, whole genome shotgun sequence.
ACCESSION   DASSGX010000424 DASSGX010000000
VERSION     DASSGX010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162770"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VFI12_07520"
     CDS             <1..237
                     /locus_tag="VFI12_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056297.1"
                     /translation="GSRVTSAIRSALHDLTKANKPYVVAGAVVGVTALQSVILRGIVQ
                     VTRRRLVAKHTRAEAMEWLANEAKEVIRQERTSA"
     gene            complement(209..892)
                     /locus_tag="VFI12_07525"
     CDS             complement(209..892)
                     /locus_tag="VFI12_07525"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HET7056298.1"
                     /translation="MSSRDRSVVGTEVDGRSVDCLLRSLWSGLAGSLAGAADASEHRR
                     IYGVNDMAKLVFGMNQSLDGYVDHTAFAPGAVLFQHFIGEAQAQSGSVYGRRMYEVMR
                     YWDDEHPEWTAAERAFASAWRKQPKWVVSRSLNSVGPNATLVDHDLEGTIRDLKTEHD
                     GEIEVAGPDLARSLTELGLIDEYRIYLHPVVLGHGKPYFAGPRPPLRLMTYDRIGEDV
                     IRLTYVPAG"
     gene            complement(927..1883)
                     /locus_tag="VFI12_07530"
     CDS             complement(927..1883)
                     /locus_tag="VFI12_07530"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7056299.1"
                     /translation="MSITAALASLVLFTAAPVHGQSLKVTLLGTGRPDPVLDRFGPAT
                     LVEAGNQTLLFDCGRGASQRLWQLKTPLSRINALFLTHLHSDHTVGIPDLWLTGWLPA
                     PFGRRTVPLRVLGPEGTRAMMSSLRQAYQWDIRVRHDGEGMPLSGIAVDARDIRQGVV
                     YERDGLKVTAFDVNHGGILRPAFGYRIDYGGKSVVISGDTRPNENLVRFASNTDVMIH
                     EVIAVRPELLEKSPDARRIVGLHTSPEDAGKIFERVKPKLAVYYHVVLLTTDPAISAP
                     TVQELVPRTRTTYSGPLEVGEDLMTILIGDSVDVRRFSPAGH"
BASE COUNT          412 a          665 c          629 g          398 t
ORIGIN      
        1 gggtcgcggg tgacgagcgc cattcgcagc gcactccacg acctcacgaa ggccaacaaa
       61 ccctatgtcg tggccggagc cgtcgtcggc gtcaccgccc tccagtcggt gattcttcgc
      121 ggcatcgtcc aggtcacccg tcgacgactc gtggcaaagc acacccgcgc cgaagcgatg
      181 gaatggctgg cgaacgaggc gaaggaggtc atccggcagg aacgtacgtc agcctgatca
      241 catcctcgcc aatccggtcg tacgtcatca ggcggagcgg cggccggggt ccggcgaagt
      301 atggcttccc gtgacccagt acgaccgggt gcagatagat ccgatactcg tcgatcaggc
      361 caagctcggt gagacttcgc gccaggtccg gaccagcaac ctcgatctcc ccgtcgtgct
      421 cggtcttcag atcgcggatc gtgccctcga gatcatggtc gaccaacgtg gcgttcgggc
      481 caacggagtt cagggagcga gagacaaccc atttcggctg ctttcgccat gccgaggcaa
      541 aggctcgttc cgcggcagtc cactctggat gctcgtcgtc ccagtaacgc atgacctcat
      601 acattctgcg cccgtacaca ctgcccgact gcgcctgggc ttcaccgatg aagtgctgga
      661 agagcacggc gcccggcgcg aacgccgtat gatcgacgta gccgtcgagg gactggttca
      721 ttccgaacac gagcttcgcc atgtcgttca ctccgtagat gcgacgatgt tcgctggcgt
      781 ctgccgcccc agccaagctg ccggcgagcc ctgaccagag cgagcgcagc aggcagtcga
      841 cagatcgccc gtcgacctca gtgccgacaa cgctgcggtc acgagaagac atcagttgaa
      901 caaactcatt ctcgactgga caagcgtcag tgcccagcag gagagaacct gcggacatcg
      961 acactatcgc caatgaggat cgtcatcagg tcctctccga cctcgagtgg tccggagtag
     1021 gtcgtgcgcg ttctcgggac gagctcctgc acggtgggcg cgctgatggc aggatctgtc
     1081 gtcagcagga cgacgtgata atacacggcc agctttggct tgacgcgctc gaatatcttc
     1141 ccggcgtcct ccggcgaagt gtggagccca acgattcttc gcgcatcggg cgacttttcc
     1201 agaagttcag gccggaccgc aatgacttcg tgtatcatca catccgtatt gctcgcgaat
     1261 cggacgagat tctcatttgg tcgggtatcc ccggagatca cgaccgactt tccgccatag
     1321 tctatgcggt agccgaatgc gggccgcagg atacctccat gatttacatc gaacgccgtc
     1381 accttcagtc cgtcgcgctc gtagaccacc ccttgccgga tatcgcgcgc atcgaccgcg
     1441 atgcctgaca gaggcatgcc ttctccatcg tgccggacgc gaatgtccca ctgatatgcc
     1501 tgtctcagtg atgacatcat cgcccgtgtg ccttcaggtc ccagcacccg taatggaacc
     1561 gtgcggcgtc cgaacggcgc tgggagccag cctgtgagcc acaagtcggg aatgccgacg
     1621 gtgtggtccg aatgcaggtg ggtcaggaat agcgcgttga ttctcgacaa cggcgtcttg
     1681 agctgccaaa ggcgctgact tgcgccgcgc ccgcaatcga aaagcagggt ttggttgccc
     1741 gcttccacca gggtggcagg tccaaagcgg tcgagaacgg gatccggtcg gcccgtaccg
     1801 agtaacgtca ctttcaagct ctggccgtgc accggagccg ccgtgaagag aacgagggac
     1861 gccagtgcag ctgtgatcga cattcgtatc atcgctttcc tcgcttggcc gattgcgaca
     1921 tggctggaac gacgccatgc aatgaacggc gggtatccgc acgacgcctt cgctcggctg
     1981 cgagcgatcg actcatatgc ggctcaccgg cgtggctgaa ctagtatatc gcgacagacg
     2041 aacaccgcat cggcttctct tcgagctcgc gttcatgagt tgcagcgatt cgctaggtcg
     2101 cccc
//
LOCUS       DASSGX010000425         2573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_946332, whole genome shotgun sequence.
ACCESSION   DASSGX010000425 DASSGX010000000
VERSION     DASSGX010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946332"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="VFI12_07535"
     CDS             <1..720
                     /locus_tag="VFI12_07535"
                     /inference="COORDINATES: protein motif:HMM:NF019952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HET7056300.1"
                     /translation="VSHLGHWRCPRCTAARPALDVRGTDIALHGVESLELTVNRPGGA
                     PIRICAGVPGMYNAYNVLATAAVAGAMGVQPPAVQTAMAEFKSAFGRIERVEVAGRTI
                     TLALVKNPVGFNEVLRMLTMATDGLNVPTMIVINDRHADGRDVSWLWDVDFELLVDGD
                     AALTTSGIRGADMANRLKYAGVPESRIQALEPDPGASIDLFVAGIPEGGRGYILATYT
                     AMLDLRANLAERGAVERFWEQ"
     gene            723..1451
                     /locus_tag="VFI12_07540"
     CDS             723..1451
                     /locus_tag="VFI12_07540"
                     /inference="COORDINATES: protein motif:HMM:NF019305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HET7056301.1"
                     /translation="MTMTFTIGWLYPSKMNIYGDRGNVIALQRRAEWRGLCARVVDIN
                     IGDPIPADIDVFFFGGGQDEQQVAVSRDLQGAKGEAIKAAIEAGAGMLAVCGGYQLLG
                     HEYRPHATNPLPGIGLFDVISVAGPERFIGNVVVESQWGDLVGFENHSGLTNLSAGQQ
                     PMGRVKVGRGNNGSDGTEGAIYKHAIGCYLHGSLLPKNPAVTDWLIESSLRRRDPGFV
                     LAPLSNDIEQAARSTAIERAVATR"
     gene            1469..1990
                     /gene="mreD"
                     /locus_tag="VFI12_07545"
     CDS             1469..1990
                     /gene="mreD"
                     /locus_tag="VFI12_07545"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HET7056302.1"
                     /translation="MARLLYALVMFAAAIFQATVMSHYSPIEIQPDITLVLLLVWCAN
                     TGTAEGLTWAFGLGILTDVLALDALGTNPLALLPVALLAAASRKRLFQSKLLFPILLT
                     VAATIAHAVILLLLRSSAVGDVPLATVFRLVVLQSLLNSIIVPPLALFATVLSGRAAA
                     RTRRYQRSSYRTG"
     gene            2001..>2573
                     /locus_tag="VFI12_07550"
     CDS             2001..>2573
                     /locus_tag="VFI12_07550"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056303.1"
                     /translation="MLFGRHSGGKKKKRIPYDPPGSKARKKANKKANRRVDQLFLTRR
                     AMLMKSAVFGGFSILLARLGYMQIGKGEDYQAAATTNITDWRELKPSRGIIVDRKGRL
                     LAKNRKSWSVSVIPADIRQLEPEQLSYVREQLMTALRLPDVVMVNPGSIPVEAKDQVY
                     RRVGRLLGDTTEQDFENTKNWIKAQLRYNDY"
BASE COUNT          450 a          838 c          834 g          451 t
ORIGIN      
        1 gtgtctcacc tcggccactg gcgctgcccg cgctgcacgg ccgcccggcc ggcactcgat
       61 gttcgcggaa ccgatatcgc cctgcacggc gtcgagtccc tggagcttac cgtgaaccgg
      121 ccgggcggtg cgccgatccg gatttgcgcc ggcgtcccgg gcatgtacaa cgcgtacaac
      181 gtgctggcca ccgccgctgt tgccggggcg atgggcgtgc agccgccggc cgtgcaaaca
      241 gcaatggccg agttcaagtc ggcgttcggc cggatcgagc gagtcgaggt cgcgggccgc
      301 acgatcaccc tggccctggt caagaacccg gtcggcttta acgaggtcct gcggatgctg
      361 accatggcga ccgacggcct gaacgtgccg accatgatcg taatcaatga tcgccacgcc
      421 gatggccgcg acgtctcctg gctctgggat gtcgatttcg agttgctggt tgatggggat
      481 gccgcgctga cgacgtcggg cattcgcggc gccgacatgg ccaaccggct caagtacgcc
      541 ggggtgccgg agagccggat ccaggcgctc gagccggacc cgggcgcatc gatcgacctc
      601 ttcgtggccg ggatcccgga aggcggtcgc ggctacattc tcgcgaccta taccgccatg
      661 ctcgatcttc gcgcaaacct ggccgagcgc ggcgcggtcg agcggttctg ggagcagtaa
      721 cgatgaccat gaccttcaca atcggctggc tttacccgag caagatgaac atttatggcg
      781 accgcggcaa tgtgatcgca ctccagcggc gggccgaatg gcgtggcctc tgcgcgcggg
      841 tagtcgatat caacatcggc gacccgatcc cggcggacat cgacgtcttc ttcttcggcg
      901 gcgggcagga cgagcagcag gtggccgtct cgcgcgattt gcagggcgca aagggcgagg
      961 cgatcaaggc ggccatcgag gcaggagccg ggatgctggc cgtctgtggc gggtaccagc
     1021 tcctgggcca cgaataccgc ccgcacgcga ccaacccgct gccgggcatc ggcctgttcg
     1081 acgtcataag cgtcgccggt ccggaacggt tcatcggcaa tgtcgtcgtc gagagccagt
     1141 ggggcgatct ggttggcttt gaaaaccaca gcggcctgac caatctctcc gccggccagc
     1201 agccgatggg ccgggtcaag gtcggacgcg ggaacaacgg ctcggacggg acagagggcg
     1261 cgatctacaa acatgcgatc ggctgctacc tgcacggctc gctcctgccc aagaatccgg
     1321 ccgtgaccga ctggctgatc gagtcctccc tgcggcgccg tgatcccggt ttcgtgctgg
     1381 cccccctctc caacgacatc gagcaggcgg cccggtccac ggcgatcgag cgcgccgtag
     1441 ccacccgata gcggcggtat cgagcgatat ggcccggttg ctgtatgccc ttgtcatgtt
     1501 cgccgcggcg atcttccagg cgaccgtgat gagccattac agcccgatag aaatccagcc
     1561 ggacatcacg ctcgtattgc tactcgtctg gtgcgcaaat accggtactg ccgagggttt
     1621 gacgtgggcg ttcggcctcg gcatcctgac cgacgtgctg gcgctcgatg cgctggggac
     1681 gaacccgctg gcgctcttgc cggttgcgct gctcgcggcg gcatcgcgga agcggctgtt
     1741 ccagagcaag ctgctctttc cgatcctgct gacggtggcg gcgacgatcg ctcatgccgt
     1801 catcctgctc ctgcttcgca gcagcgcggt cggggatgtg ccgctggcaa ctgtcttccg
     1861 gctcgtcgtt ttgcagtcgc tgttaaactc gatcattgtt cctccgcttg cgttgtttgc
     1921 cacggtgttg agtggccgcg cggcagcacg cacccgcagg taccaacgct cgtcgtatcg
     1981 cacaggctga ttggctggca atgcttttcg gacgacactc aggcggcaaa aagaagaaac
     2041 gaatcccgta cgatcccccg ggaagcaagg cgcgcaaaaa ggccaacaag aaggccaacc
     2101 gccgcgtcga tcagctcttc ctgacccgcc gcgccatgct gatgaaatcg gccgtgttcg
     2161 gcggcttcag catcctgctc gcccgcctcg gctacatgca gatcggcaag ggcgaggact
     2221 accaagccgc cgcgaccacc aacatcaccg actggcgcga gctgaagcct tcacggggca
     2281 tcatcgtcga ccgcaagggc cggctgctgg ccaagaaccg caagtcatgg tcggtcagcg
     2341 tcattccggc cgatatccgc cagttggagc cggagcaact ctcatacgtg cgcgagcagc
     2401 ttatgacggc gctgcgcctg ccggacgtgg tcatggtcaa tccgggttcg atcccggtcg
     2461 aggccaagga ccaggtctac cggcgcgtcg gccgcctcct tggagacacc accgagcagg
     2521 attttgagaa cacgaagaac tggatcaagg cgcagctccg gtacaacgac tat
//
LOCUS       DASSGX010000426         2397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1491685, whole genome shotgun sequence.
ACCESSION   DASSGX010000426 DASSGX010000000
VERSION     DASSGX010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1491685"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VFI12_07555"
     CDS             <1..835
                     /locus_tag="VFI12_07555"
                     /inference="COORDINATES: protein motif:HMM:NF014801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HET7056304.1"
                     /translation="NAGLIARASAEWGGVPITGCAGDQHAALFGQACFDSGQAKNTYG
                     TGSFALMNTGSDVVASNNGLLTTPAWVLGDQATYALEGSVLVSGSAVQWLRDELGIIG
                     SAAEIEPLARSVPDSGGVVFVPAMTGLGAPDWDDAARGTILGITRGTTRAHIARATLE
                     AIAFQVTDVLRAMGGDAGSPVTELKVDGGAARNDLLLQLQAEFLGVPVVRSAVQETTA
                     LGAAYLAGLGVGYWSDTDVINRMWKSDGRFEPSLSADERESRIATWRRAVDRSRGWAL
                     P"
     gene            complement(832..1770)
                     /locus_tag="VFI12_07560"
     CDS             complement(832..1770)
                     /locus_tag="VFI12_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056305.1"
                     /translation="MRLRPGAKDGGTKTGKVGGARQLVSLIRGLSIEEIEASVRQPPR
                     ILFVSREPDIGDLIEELTGVRGTPTLEIIDPDRLPKDLLQYDVIVVHSPSSNEDYLKV
                     RKRAGVAAHRVFDAGPSVNINELRNRITEQAGNGTAALGRWYPGFRDAAASTIINETS
                     RENAQFALMSSVPSVIPVIGAIASAGADMIILTKNQLLMAIKLAAVHGKPLGDKREIF
                     RDLLPVIGSGFLWRTIAREAVTFLPFAAGTVPKVAIAFAGTFATGRAIDTYYRFGNKP
                     TKDQLNSYYRQAMELVKSKFGKSKPLPEPDTAAPPG"
     gene            complement(1797..>2397)
                     /locus_tag="VFI12_07565"
     CDS             complement(1797..>2397)
                     /locus_tag="VFI12_07565"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642298.1"
                     /note="Catalyzes the reversible oxidation of malate to
                     oxaloacetate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HET7056306.1"
                     /translation="VIVVSNPLDAMCHVAFDAAGFPRERVIGMAGVLDSARFRAFIAM
                     EMGVSVDDVSAMVLGGHGDTMVPLSRYSTVAGVPITELLPADRVTAIEERTARGGAEI
                     VGLLKTGSAYYAPGMSTVEMVDAIMLDKKRILPCSVLLKGEYGINDLYVGVPIKLGAG
                     GVEQIIELNLTGDESAALKKSSGAVKELVDAMASIRASS"
BASE COUNT          386 a          784 c          796 g          431 t
ORIGIN      
        1 gaatgccggc ttgatcgccc gcgcctcggc cgagtggggc ggagtcccga tcaccgggtg
       61 tgccggcgat cagcatgcgg cgctgttcgg ccaggcctgt ttcgattccg gccaggccaa
      121 gaatacgtat ggcaccggtt ccttcgcctt gatgaacacc gggagcgatg tagtcgcgag
      181 caacaacgga cttctgacga cacccgcgtg ggtcctcggc gatcaagcca cgtacgcact
      241 cgaggggtcg gtcctggttt cgggttcggc cgtgcaatgg ttgcgcgatg agctgggaat
      301 catcggctcc gccgccgaga tcgagccact tgcccgctcg gtgccagatt ccggaggcgt
      361 agtcttcgtc ccggcaatga ccgggctcgg cgcaccggat tgggacgacg cggcccgggg
      421 cacgatcctg ggcatcacgc gggggacgac cagggcgcac atcgcccggg ccacgctcga
      481 ggcgatcgcc ttccaggtga cagacgtgct gcgtgcgatg ggcggcgacg ccggatcgcc
      541 cgttaccgag ctcaaggtcg atggcggcgc tgcccggaac gacctgctgc tccagctcca
      601 ggctgaattc ctcggcgtgc cggtcgtgcg gtccgcggtg caggagacga ccgcgctggg
      661 cgcggcatat ttggccggat tgggcgtggg gtactggtct gatacggacg tgatcaaccg
      721 catgtggaag agcgacggcc gcttcgagcc gtcgctatcg gctgatgaac gggagtcccg
      781 gatcgcgacc tggcggcgcg cagtggaccg atcgcgcggc tgggctttgc cttaacccgg
      841 aggggcggcg gtatccggct cgggcaacgg cttcgatttg ccgaacttcg atttgaccag
      901 ctccatggcc tgccggtagt agctgttgag ctgatccttg gtcggcttgt tcccgaaccg
      961 gtaataggta tcgattgctc tgccggtggc aaacgtccca gcgaaggcga tcgccacttt
     1021 cggcactgtc ccggctgcga aaggcaggaa cgtgaccgcc tcgcgcgcga tcgtccgcca
     1081 caggaagccc gatccgatca cgggcagcag gtcccggaag atctcgcgct tgtcgccaag
     1141 cggcttaccg tgaactgccg ccagcttgat cgccatcagc aactgattct tggtcaagat
     1201 gatcatgtcg gcgccggccg aggcgatcgc gccgatcacc ggaattacgc tcggcacgct
     1261 ggacatgagc gcgaactggg cattctcgcg cgatgtctcg ttgatgatcg tgctggcggc
     1321 cgcatcgcgg aaaccggggt accagcgacc gagcgcggcc gtcccgttgc cggcctgctc
     1381 ggtgatccgg ttgcggagct cattgatatt gaccgacggg ccagcatcga agacgcgatg
     1441 cgccgcgacg ccggctcgtt tgcggacctt gaggtaatcc tcgttcgagg acgggctgtg
     1501 gaccacgatg acgtcgtact ggagcagatc cttcggcagg cgatccggat cgatgatttc
     1561 gagcgtgggg gtgccccgca ccccggtcag ctcctcgatc agatcgccga tgtccggctc
     1621 gcggctgacg aacaggattc gcggcggctg gcggaccgat gcctcgatct cctcgatgct
     1681 cagcccgcga atcagggaga cgagctgccg cgcgcccccg actttgcccg ttttcgtgcc
     1741 gccgtccttc gctcccggtc gtaaccgcac gctcgctcct tcccgatatc cggcttctac
     1801 gatgatgcgc ggatcgaggc catcgcgtcg accagctcct tcacggcgcc ggatgacttc
     1861 ttgagcgctg ccgactcgtc tccagtcagg ttcagctcga tgatttgctc gaccccgccg
     1921 gcgccgagct tgatcggcac gccgacatag aggtcgttga tgccgtactc gcccttgagc
     1981 agcacgctgc acggaaggat gcgcttcttg tcgagcatga tcgcgtcgac catctcaacc
     2041 gtagacatgc ccggcgcgta gtaggcgctg ccggtcttca gcaggccgac gatctcggcg
     2101 ccacccctgg ctgttcgctc ctcaatcgcc gtcacccggt cggccggcag cagctcggtg
     2161 atcggcactc cggcgaccgt cgagtagcgg gagagcggca ccatcgtgtc gccgtggccg
     2221 ccgaggacca tcgcgctcac gtcatcgacc gagacgccca tctccatcgc gatgaacgcc
     2281 cggaagcggg ccgagtcgag cacgccggcc atgccgatca cccgctcgcg cgggaagccc
     2341 gccgcgtcga aggcaacgtg gcacatcgcg tcgagtggat tggagacgac gataacc
//
LOCUS       DASSGX010000427         2074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1163610, whole genome shotgun sequence.
ACCESSION   DASSGX010000427 DASSGX010000000
VERSION     DASSGX010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1163610"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VFI12_07570"
     CDS             <1..531
                     /locus_tag="VFI12_07570"
                     /inference="COORDINATES: protein motif:HMM:NF025713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HET7056307.1"
                     /translation="AGSETIQAAQRAMGTVGWQLDASGDLYPQRIAGLEGRALSGALR
                     QYVRRIQQAGDDDALTIGAAKELLEAAARHVMVESTGSYDPKADFPTTMYRAATTLGT
                     GTPTAGMIQDLDDDPARALEQALALVALTISRLRNAEGSGHGRPQMTRATKRQGIIAA
                     NAAAAVAYVLLKDLTD"
     gene            977..1285
                     /locus_tag="VFI12_07575"
     CDS             977..1285
                     /locus_tag="VFI12_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056308.1"
                     /translation="MNAIAVGNRLALNNGSHRAYALYEAGFRNVPCVVQRVEHREELM
                     IVASGDLVSNPDRYLADPRPPLLRDYFDDQLRTPVPVPRKFRELRLQFGFEQVDVPAR
                     "
     gene            1473..1799
                     /locus_tag="VFI12_07580"
     CDS             1473..1799
                     /locus_tag="VFI12_07580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HET7056309.1"
                     /translation="MAAARFLVIWDTPKDPEAFDRHYREIHIPLANRLAGLRSYTVGR
                     NLVAVRGGDPPYIVGELEWDDIASLRAAFASPEGQATAADVNELSANASVRSMIYEVL
                     PSTELD"
     gene            complement(1840..>2074)
                     /locus_tag="VFI12_07585"
     CDS             complement(1840..>2074)
                     /locus_tag="VFI12_07585"
                     /inference="COORDINATES: protein motif:HMM:NF021758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rdx family protein"
                     /protein_id="HET7056310.1"
                     /translation="YCAECGYEPQTLALAEALMRRFGPDLAAIEIVPWLDGTFEVSVD
                     GQLVHSMARDGGFPTNQAIIDAVAESAGAISPP"
BASE COUNT          389 a          681 c          650 g          354 t
ORIGIN      
        1 gccggtagtg agacgatcca ggcggctcaa cgagccatgg gcactgttgg ttggcagctc
       61 gacgcatctg gcgacttgta tccgcagcga atcgcgggtt tggaaggccg agcgctttct
      121 ggggccttga ggcaatacgt gcgccgaatt cagcaggccg gcgacgacga cgccctcacg
      181 atcggcgcag ccaaggagct gctggaagcg gctgcgcgcc acgtgatggt cgagtcaaca
      241 ggttcgtacg acccgaaagc cgatttcccc accacgatgt accgagcagc gacaacattg
      301 gggaccggca ccccaacggc gggcatgatt caggatctcg acgacgaccc agcgagagcc
      361 ctcgagcagg cgctcgcgct agttgcattg acgatcagcc gtctccgaaa tgcggaaggg
      421 tccggacacg gacggccaca gatgactcga gcgacaaagc gacagggaat catcgccgct
      481 aacgcggctg cagcggtcgc gtacgttctg ctcaaggacc tcacggatta gacggggacg
      541 accgccagtc tcgcatcgac gtgacgccgt ctgggcacag tcgggctgcg tcgccagcca
      601 gtgacaggca acctggcaca cggtggccaa aggattgacc cggccgatcc gcagcgcagg
      661 gactttagaa catgatcgag cctccgatcg acgaattcct atttctttcc ggccgaccac
      721 ctcttcagga atacctgggc tttattgccc agcagaccac ggacgagcac ctaatggacg
      781 tgggccagct gatcaacggg tggcgtcggt atcggtgacc cgcgcgaatc agaacacatg
      841 gattgccgtg tcgccgtcga ccgaccttcg cttcctaggc ctagcgttct tgccccctga
      901 ccacgtcccc ggctatccgt caactgggta tccaaccgtc gtgctagctc tcggcatcgg
      961 ctatggatca aacaccgtga acgctattgc ggtcggcaat cgcctcgctc tcaacaacgg
     1021 cagccaccgc gcatacgccc tctacgaggc cggtttccgc aacgtgccct gcgtagtgca
     1081 gcgcgtcgag catcgcgagg aactgatgat agtggcgagc ggtgacctgg taagcaatcc
     1141 agaccgatac cttgccgatc cgcgcccacc attgctacgc gactatttcg acgatcagct
     1201 gcgaaccccc gtaccggtgc cccgaaagtt tcgggaactg cggctccagt ttggcttcga
     1261 gcaggtggat gtccctgccc gatgagccag tgccctcaga cataacttac cttatcggaa
     1321 gtagtgtcag acgttcgggg gaccgttcgg cgtcgtcgtg accagcggtg cctgtcggac
     1381 gcagagatcg ctgtcacgga tctgcctcga acgacggtcg cgctgcgctg tcggctcggg
     1441 caggcttcga ccaaccgcac acggaggcgc acgtggccgc tgctcgattc ctcgtcatct
     1501 gggacacgcc gaaggatccc gaggcgtttg atcgccacta ccgcgagatc cacatcccgc
     1561 tcgcgaaccg tctggctggc cttcggagct acacggtcgg ccgcaacctc gtggccgtcc
     1621 gcgggggtga tccgccgtac atcgtcggcg agctcgagtg ggatgacatc gcgtcgctcc
     1681 gagcggcgtt cgcctcaccg gagggtcagg cgacggccgc cgacgtcaac gagctgtcgg
     1741 ccaatgcctc ggttcgcagc atgatctacg aggtcctacc gtcgaccgag ctcgactgac
     1801 cagggcgctc ccgggccgcc aggcgccgga aggtctacgt cacggcggag agatggcgcc
     1861 tgccgactct gccacggcat cgatgatcgc ctggttcgtc gggaatccgc cgtcgcgggc
     1921 catcgagtgc acgagctggc cgtcgaccga cacctcgaac gtgccgtcga gccagggcac
     1981 gatctcgatg gccgccaggt ccgggccgaa acgccgcatc agcgcctccg ccagggcgag
     2041 ggtctgcggc tcgtagccgc actcggcgca gtag
//
LOCUS       DASSGX010000428         3200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_836431, whole genome shotgun sequence.
ACCESSION   DASSGX010000428 DASSGX010000000
VERSION     DASSGX010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836431"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..1132)
                     /locus_tag="VFI12_07590"
     CDS             complement(197..1132)
                     /locus_tag="VFI12_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056311.1"
                     /translation="MPNGMADTRAFLRSMGLPEGDLHDLPASPKRFPDGGQWRIEIPS
                     VEGPNALQAVYDTADELEVPIHRVSQGSGVMLQTDDELDSMAKLGRARHIEISLFVGP
                     RAGWDTGAMATSTAGRVVAPKIRGMDQLVQAVEDVRRSCEHGINSVLVTDEGLLWVLG
                     AMKKGGELPTDLIIKGSVMIGSCNPASIKLLEERGMTTFNVPTDLSLAHLAAIRSVVD
                     MPLDIYVEAPDDIGGFIRHYEIAELVRVCAPVYLKFGLRNAPNIYPSGTHLEATAIAL
                     TKERVRRAKLGVDLLRRLDPDAVMSPLPGDGERPG"
     gene            complement(1334..1792)
                     /locus_tag="VFI12_07595"
     CDS             complement(1334..1792)
                     /locus_tag="VFI12_07595"
                     /inference="COORDINATES: protein motif:HMM:NF022279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrdB family protein"
                     /protein_id="HET7056312.1"
                     /translation="MAGRTLASCSGASINRTPRQRTIIPSGYHPIPAIHLAIRFILEI
                     AMLAGVWMAGTRVWGYPGAILGILAAMALWGVFGALGDGSRGAPVVPTPGQLRLLLEI
                     ALFGIAAYGYWVEWSRAASETFLTISILHYAITWERQWWLIRGGPLPGGD"
     gene            complement(1749..3008)
                     /locus_tag="VFI12_07600"
     CDS             complement(1749..3008)
                     /locus_tag="VFI12_07600"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HET7056313.1"
                     /translation="MMPRRVVVTGVGAVTSLAPTAKQTWEKVLAGESGLKRIDLDPDQ
                     NPCLVRGDVDDATISNRFLDGKTLRNTSRFSRMAVEAAGEALIDAGLIDEEGSPCIDL
                     SDAGTVIGTCVGGAHDDLLPAWTTYENRGPGRVPPHLHVMFPLNLASYTIQWRWGMQG
                     PTNTVNTACATGSQAIGEAFHQVKFGRADIMVAGAVESDSHPMFIAGFAAMKALATDS
                     NDNPSGASRPFDKSRAGFVLGEGAGMVVLEEYEHAKARGAEMYAEVLGFASTNDAFHP
                     IAPRPDGSGSAKAVRNALKDAGVEPDEVGHVQAHAASTPVGDVAEANAIKEVFGDRAT
                     GIPVTSIKGAIGHCMGAAGSIESVMAILSMANQTIPPTLNYTTPDEEIGLDIVHGEAR
                     TADFAVSTKHSFGLGGQNACLVLGRID"
BASE COUNT          542 a         1080 c         1035 g          543 t
ORIGIN      
        1 ggcagggcac cgcgaccttc atctcccgcg gcatcgacca gggcggcatc gtcatcatcg
       61 ccatccgccc cccggaatag cgtcgggaag cggcgccgcg acccatgtag gcccggcccg
      121 aacctgcaaa tgtaggggca gatggcatct gccctccgcc cctacatccg ttccctcaac
      181 cctccaccct caacgctcaa cccggccgct cgccgtcccc cggcagcggc gacattaccg
      241 cgtccggatc caggcgccgc aacaggtcga cccccagttt ggcccggcga acgcgctcct
      301 tcgtcagggc gattgccgtc gcctcgaggt gcgtgccgga ggggtagatg ttcggcgcgt
      361 tgcggaggcc gaacttgagg tagaccggcg cgcagactcg gaccaactcg gcgatctcgt
      421 aatggcggat gaagccgccg atgtcgtcgg gcgcctcgac gtagatgtcg agcggcatgt
      481 cgaccaccga tcggatcgcg gcgaggtgcg caagggaaag gtcggtcggc acattgaacg
      541 tcgtcatgcc tcgctcttcc aggagcttga tcgacgccgg gttgcacgag ccaatcatga
      601 ccgagccctt gatgatcaag tcggtgggca gctcgcctcc tttcttcatg gccccgagca
      661 cccagagcaa tccctcatcc gtgaccagca cgctgttgat gccgtgctcg cacgagcggc
      721 gcacatcctc gaccgcctgg acgagctggt ccatgccacg gatcttcggc gcgacgaccc
      781 ggccggccgt tgaggtcgcc atcgccccgg tgtcccaacc ggcgcgagga ccaacgaaga
      841 ggctgatctc gatgtgccgg gcgcggccga gcttcgccat cgagtccagc tcatcatcgg
      901 tctggagcat gacgccgctg ccctggctga cccggtgaat cggcacctcc agctcgtcgg
      961 ccgtgtcgta gaccgcctgg agcgcatttg gcccctcgac cgacgggatc tcgatccgcc
     1021 actggccacc gtccgggaag cgcttcggag aagcgggcag gtcgtgcaga tcgccttcgg
     1081 gcaaccccat cgatcgcagg aaagcgcggg tgtcagccat gccgttcggc atcggaaacc
     1141 tccagtaatt gcggtcgatc aacgaccgga agacataccg gcgtcatgct aacgcggact
     1201 tcgcggttgg tgttcgcctt taggcgtagt tccgggcggc ctcgcccggc ttctcgctgg
     1261 gatgatgccg ggtaagaccg cccggaacta cattgcagga agagtcgcag cggtagttgg
     1321 ctctctgcct cgatcagtct ccgcccggga gcggtccgcc ccggatcagc caccactgcc
     1381 gctcccacgt gattgcgtag tgcaggatcg agatcgtcag aaacgtttcc gacgcggccc
     1441 gggaccattc aacccagtaa ccgtaggcgg cgatcccgaa gagcgcgatc tcgagcagga
     1501 ggcgaagttg ccccggcgtc gggacaaccg gcgcgccacg cgagccatcg ccgagggcgc
     1561 cgaagacgcc ccaaagcgcc atcgcggcga gtatgccgag gatcgcgccg ggatagcccc
     1621 aaacgcgcgt gcctgccatc cagaccccgg ccagcatcgc gatctcgagg atgaagcgga
     1681 tcgcgaggtg aatggccggg attggatgat acccggacgg aatgatcgtc ctttggcgag
     1741 gcgtgcggtt aatcgatgcg cccgagcacg aggcaagcgt tctgcccgcc aaggccgaac
     1801 gaatgcttgg tcgagacggc gaagtcagcc gtgcgcgcct ccccgtgcac gatgtccagg
     1861 ccgatctcct cgtccggcgt cgtgtagttg agcgtcggcg gaatcgtctg gttcgccatc
     1921 gagaggatcg ccattaccga ctcgatcgag ccggccgcgc ccatgcagtg gccgatcgcg
     1981 cccttgatcg acgttaccgg aatcccggtt gcgcgatcgc cgaagacttc cttgatcgcg
     2041 ttcgcctcgg caacgtcgcc gaccggcgtc gaggcggcat gcgcctggac gtggccgact
     2101 tcatccggct cgactccggc gtccttcagc gcgttgcgca cggccttggc cgagccgctg
     2161 ccatccggcc gcggcgcgat cgggtgaaac gcgtcgttcg tcgaggcgaa gccgagcact
     2221 tcggcataca tctccgcccc gcgcgccttt gcgtgctcgt actcttcgag cacgaccatg
     2281 ccggcgccct cgccgaggac aaagccggcc cggctcttgt cgaacgggcg gctggcgcca
     2341 ctcgggttgt cattgctgtc ggtcgcgagc gccttcatcg cggcgaatcc ggcaatgaac
     2401 atcgggtgcg agtcggactc gaccgcgccg gcaaccatga tgtccgcccg gccgaacttg
     2461 acctggtgga atgcctcgcc gatcgcctgc gagccggtgg cgcaggcggt gttgaccgtg
     2521 ttggtcggcc cttgcatgcc ccagcgccac tggatcgtgt agctggccag gtttagcggg
     2581 aacatgacgt gcaggtgcgg ggggacgcgc cccgggccgc ggttctcgta ggtcgtccag
     2641 gcgggcagca agtcatcgtg cgcgccgccg acgcaggtgc cgatcacggt gccggcatcc
     2701 gacaggtcga tgcacggcga accctcttcg tcgatcaggc cggcgtcaat cagcgcctcg
     2761 ccggccgctt cgaccgccat tcgcgagaag cgagaggtgt tgcgcagcgt ctttccgtcg
     2821 aggaaccggt tgctgattgt cgcgtcatcg acatccccgc gcacgagaca cgggttctgg
     2881 tccgggtcga ggtcgatccg cttcagaccg ctctcgccgg ccagcacctt ttcccaggtc
     2941 tgttttgcgg tcggagcgag cgaggtgacc gcgccgacgc cggtgacgac gacccgccgg
     3001 ggcatcatgg gcgaccgttc agaagcagat gacggagatg acatggcctt ccattcacaa
     3061 ggtcaacgac accgggacag gtgtacttgc gctacaccat tttgcgcaac gcaggctcag
     3121 tataccggtg gtcctggtcg aaatagtccg atcgtatagg ccgaaaccgc gacctgcgtc
     3181 actaaacgta cactggaggc
//
LOCUS       DASSGX010000429         2449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_727326, whole genome shotgun sequence.
ACCESSION   DASSGX010000429 DASSGX010000000
VERSION     DASSGX010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727326"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..1079)
                     /locus_tag="VFI12_07605"
     CDS             complement(150..1079)
                     /locus_tag="VFI12_07605"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HET7056314.1"
                     /translation="MPPASWSERQARAVARSIIEFHLDTPPDRLVQMGGGLSNWVYTA
                     YHAKGAFIVRFGAAAEKIEDFLKEQWAMARAREKGVPTPEILQVGTEPVSVPYMVSRR
                     VEGRIGTHHPQRLAIAREMGRVLATIHTIPTSGYGTTFDWSQNILSRNETWGDFLRDE
                     WEVDDRMAMLRDLDLLDARQSRTLHETVEEMALWSTSPVLNHGDMRLKNVLVDTEGNI
                     VAIIDWEDARSCPPPCWDLALALHDLSIDAKEALVEGYGLTGDQMRHEAPYLKALNVL
                     HYAPIAKRLATHGNRRELDRLRTRLSGAYDLFV"
     gene            complement(1083..2003)
                     /locus_tag="VFI12_07610"
     CDS             complement(1083..2003)
                     /locus_tag="VFI12_07610"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HET7056315.1"
                     /translation="MSGEPWREALRDFASSSRFRNGGGIVTDLDGTAVHEHEGRVTIP
                     HGVSHALERLSEQGHPIVINTLRFPMSVIGTFGQEWYAITNAPLPLISLNGSLTGHLT
                     ESRTGAIVFEETEACVLSHTEIGQILTKVEGLISSGLNRLLVFYYPRDWRLGEVVWTP
                     DCERMAHVRHKYLSASEVLCGDIERLRRRLMQRDMCMVFLLVEHPRDELMAYQHVRPS
                     DFITSANVDKRDGLCRLAAHMGADPADWLGAGDTPMDNFLQDVGLAVHVGPIELAYSG
                     RRHTARIRDSIELGVLLNELSRVDEKAAAE"
     gene            complement(2105..>2449)
                     /locus_tag="VFI12_07615"
     CDS             complement(2105..>2449)
                     /locus_tag="VFI12_07615"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HET7056316.1"
                     /translation="PFLFLLGINLALFVIGMFIETSAAIIVLAPILVPVAVGFGIDPV
                     HLGLVMVINLALGMVTPPFGVNLFAACTVAKISVDEIIGKLIPFVLVVLACLMVITYV
                     PQLSLFLRDALY"
BASE COUNT          448 a          799 c          778 g          424 t
ORIGIN      
        1 atattgggtc ggcggccggc ctcaacaggt cgagttcgcc gagatccaac aggccatcga
       61 aaccggaacg cagcctgacc tcggcgccaa cgagaaggtg cagttctgaa ttggctcgac
      121 ggtggttccg tcgaggcgtt ccgacctgat cacacgaaga gatcgtacgc cccgctcaat
      181 ctcgtccgca gacggtccag ctctctcctg tttccatgcg tagcgagacg cttggcgatc
      241 ggcgcgtagt gaaggacgtt gagcgccttc agataaggtg cctcgtgcct catctggtcg
      301 cccgtcaggc cgtagccctc gacgagcgcc tccttcgcgt cgatcgacag gtcgtgcagg
      361 gcaagtgcca aatcccagca cggcggcggg cacgagcgtg cgtcctccca gtcgatgatg
      421 gcgacgatgt tgccctcggt gtcgacaaga acgttcttca gccgcatgtc gccgtggttg
      481 agaacggggg acgtgctcca tagtgccatc tcctccactg tctcgtgcag ggtgcgggac
      541 tgccgcgcat cgagcagatc aaggtccctc agcatcgcca tgcggtcgtc gacctcccac
      601 tcgtctcgca gaaagtcgcc ccacgtctca ttgcgcgaaa ggatgttttg cgaccagtcg
      661 aacgtcgtcc cgtagccgct cgtcgggatc gtgtgaatgg tggcgagcac ccggcccatc
      721 tctcgcgcga tcgccagccg ctgtgggtga tgcgtcccga tccggccctc cacgcggcgt
      781 gagaccatgt agggcacgga gaccggctcg gtgccgacct gcaggatttc aggcgtcggc
      841 acgcccttct cgcgcgcccg cgccatcgcc cactgctcct tcaggaagtc ctcgatcttc
      901 tccgcggccg ccccgaaccg cacaatgaac gcgcccttgg cgtgataggc cgtatatacc
      961 caattactca atcctccgcc catctggacc aggcgatcgg gaggagtatc gagatggaac
     1021 tcgatgatgc tgcgggcgac tgccctggcc tgacgctcac tccaggatgc cggcggcatg
     1081 gtctactcag ccgctgcctt ctcgtcgaca cgggagagct cgttgagcaa gacgccgagc
     1141 tcgatggaat cgcggatcct ggcggtgtga cgccggccgg agtaggccag ctcgatcggc
     1201 ccgacgtgca ccgccaggcc gacatcctgc aggaagttgt ccatcggggt gtcgccggct
     1261 ccgagccaat cggcagggtc cgcccccatg tgagcggcca gacggcatag cccatcgcgc
     1321 ttgtcgacat tcgcgctggt gatgaagtcg ctcggccgga cgtgctggta ggccatgagc
     1381 tcgtcgcgtg gatgctcgac cagcaggaaa accatgcaca tgtcgcgctg catcagcctc
     1441 cggcgcaagc gttcgatgtc gccgcagagg acctcggagg cgctcagata cttgtgccgg
     1501 acgtgggcca ttcgctcaca gtcgggagtc cagacgacct caccgagccg ccagtcgcgc
     1561 ggatagtaga acacgagcag ccggttcagg ccgctgctga tcaacccttc gaccttggtc
     1621 aggatctggc cgatctcggt atgagacagg acgcacgcct ccgtctcctc gaagacgatg
     1681 gctccggtgc ggctctccgt gagatggccg gtcaggctgc cgttgaggga gatgaggggc
     1741 agcggtgcat tggtaatggc gtaccactcc tggccgaagg tgccgatgac gctcatcgga
     1801 aagcgcagcg tgttgatgac gatcggatgc ccctgctcgc tgaggcgctc gagcgcgtgc
     1861 gagacaccgt gcggaatggt cacgcggccc tcgtgctcgt ggacggccgt tccgtcgaga
     1921 tccgtgacga tgccgccgcc attgcggaac cgcgatgacg acgcgaagtc gcgcagtgcc
     1981 tccctccagg gctctccgct catgaacggc cccattgctc gcgatgatcg tgaccggtca
     2041 accgccgcgc agcagcaatc gttcccgggc cgcagccgcc gcctcgggct tcagcgtcgg
     2101 gtgatcagta aagtgcgtcc cgcagaaaga gcgagagctg cggcacgtac gtgatcacca
     2161 tgaggcacgc gaggaccacc agcacgaacg ggatcagctt gccgatgatc tcgtcgacag
     2221 agattttcgc caccgtgcag gcggcgaaca gattgacccc gaacggcggg gtcaccatgc
     2281 ccagcgccag gttgatgacc atcaccagcc cgaggtgcac cggatcgatg ccgaagccca
     2341 ccgccaccgg cacgagaatc ggcgccagca cgatgatggc cgccgaggtc tcgatgaaca
     2401 tgccgatgac gaacagcgcc aggttgattc cgagcaggaa caggaacgg
//
LOCUS       DASSGX010000430         3618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_508923, whole genome shotgun sequence.
ACCESSION   DASSGX010000430 DASSGX010000000
VERSION     DASSGX010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3618
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508923"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1857)
                     /gene="gyrB"
                     /locus_tag="VFI12_07620"
     CDS             complement(<1..1857)
                     /gene="gyrB"
                     /locus_tag="VFI12_07620"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HET7056317.1"
                     /translation="MATTRAKTKTDYDASSIQILEGLEAVRRRPGMYIGSTDHRGLHH
                     LVREIVDNSVDEALGGYCDHIVTTISTDGVVTVVDNGRGIPVDKHSKMNKSALEVIMT
                     VLHAGGKFGGGGYKVSGGLHGVGASVVNALSEWMEVEVRRGGKVYKQSYKRGVPDAPV
                     KVVGTSAPDEHGTTTRFFADTSIFTAGLEYDYDALIQWFRETAYLTKGLRLTLKDERN
                     DQEMTFYFEGGIISFVRHLNRNRIPVHDRPFYVSRDTPSGLVEVALQYNDSYGESTHT
                     FANNINTSDGGTHLSGFKSALTRTINDYGKKNGFLKNDESLSGEDVREGLTAIVSVKL
                     AEPQFEGQTKGKLGNAEVAGAVQTVVNESLGAFLEENPQTAKRIIDKCLTAARAREAA
                     RKARELVQRKGGLESFSLPGKLADCSERDPSRAELYIVEGDSAGGSAKQGRDRRFQAI
                     LPLRGKILNVEKARLDKILQNNEIRTLITALGTSVGDQFDISKLRYHRVIIMTDADVD
                     GAHIRTLLLTFFYRHLEQLIHDGYLYIAQPPLYRVKSGKEEIWTYTEEEREAAIKKLG
                     ERAEVQRYKGLGEMNPEQLWDTTMDPAKRTLMQVKIEDGVSVDETFDMLMGSA"
     gene            complement(1868..2758)
                     /locus_tag="VFI12_07625"
     CDS             complement(1868..2758)
                     /locus_tag="VFI12_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056318.1"
                     /translation="MSDGMSDVLAYPQLVLIPVLLDLYLLFGARISAGSLTSRLGDWF
                     AGRSGQNALDAGKWVRDLGDWDVSRLLALLMPSVLDGMGQDTYYRPFDRLAWSPSSLG
                     VVAASIGMILIGSVIFTIYITMLASRTRMIRANSLPPMRLLLDRWVKMLGLFGLLIGV
                     IASLSAVMIVPAAILAAGGVSATTMAGLAWLVGSIALVVTMFAPEAIVIDGASPIAAV
                     RASAQVVFRFFWQSAAFFAVSLMISPGLLSIWERIAGDPVGLGIAIVLNAAMVTSLSL
                     ASLAFYRARFDGVAPLQQTI"
     gene            complement(2815..3186)
                     /locus_tag="VFI12_07630"
     CDS             complement(2815..3186)
                     /locus_tag="VFI12_07630"
                     /inference="COORDINATES: protein motif:HMM:NF018021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HET7056319.1"
                     /translation="MRTAASTLKRSRRLRFEKLVDRILTQLDPAVRLALDNLVVVVEA
                     APPDGDPEQFGVYDGVAKTERGSGYTFAVPDRIVIFQKPLEEAFPDPRELEEQIRITL
                     LHEIGHHLGFDEEQLDQLGLA"
     gene            complement(3261..>3618)
                     /locus_tag="VFI12_07635"
     CDS             complement(3261..>3618)
                     /locus_tag="VFI12_07635"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HET7056320.1"
                     /translation="KPILVDAVHNPDGSISTRTEPSIWRRPISSQTADTVLSMLYESV
                     GYGYARRAAIAGINVAGKTGTAESGREAPHGWFIGSAGVDAPAIVVSVCLDYGGEGGG
                     LALDIGRELLAAAVGP"
BASE COUNT          660 a         1150 c         1106 g          702 t
ORIGIN      
        1 agcgctgccc atcagcatgt cgaacgtttc gtcgacgctc acgccgtcct cgatcttgac
       61 ctgcatcagt gtgcgctttg ccggatccat cgtcgtgtcc cagagctgct ccgggttcat
      121 ctcgccgaga cccttatagc gctgcacttc cgcccgctcg ccgagcttct tgatcgccgc
      181 ctcgcgctcc tcttccgtgt acgtccagat ctcttccttg ccactcttga cgcgatacag
      241 cggcggctgc gcgatgtaga ggtagccgtc gtggatgagc tgttccaggt gccggtagaa
      301 aaaggtcagc agcagcgtcc ggatgtgggc tccgtcgaca tccgcgtcag tcatgatgat
      361 gacgcggtgg tagcggagct tgctgatgtc gaactggtca cccacggagg tgccgagcgc
      421 cgtgatcagc gtccggatct cgttgttctg gagaatcttg tccagtcgtg ccttctctac
      481 gttcaggatc ttgccgcgga ggggcaggat cgcctggaaa cggcgatcgc ggccctgttt
      541 ggccgaacca ccggccgagt caccctcgac gatgtacagc tcggcgcgcg acggatcccg
      601 ctctgagcag tcggccagct tgcccggcag gctgaagctt tccagcccgc ccttgcgctg
      661 aaccagctcg cgagccttgc gcgccgcctc gcgggctcgc gcggccgtca gacacttgtc
      721 gataatccgc ttcgccgtct gcggattctc ctcgaggaag gccccgagcg actcgttgac
      781 gaccgtctgg acggcgccag caacctcagc gttgccgagc ttgcccttcg tctggccctc
      841 gaattgcggt tcggccagct tcacggagac gatcgcggtc aggccctccc ggacgtcctc
      901 gccggagagc gactcgtcgt tctttaggaa cccgttcttc ttgccgtagt cattgatcgt
      961 gcgggtcaac gccgacttga agccggagag gtgcgtgccg ccatcgctcg tgttgatgtt
     1021 gttcgcgaac gtgtgtgtcg actcgccgta gctatcgttg tactggagcg caacctcaac
     1081 caggccggac ggcgtgtctc gcgagacgta gaacggtcga tcgtggaccg ggatccggtt
     1141 ccggttgaga tggcgcacga aggagatgat gccgccttcg aaatagaagg tcatctcctg
     1201 gtcgttccgc tcatccttca gcgtcaggcg caaccccttg gtcaggtacg ctgtctcgcg
     1261 gaaccactgg atcagcgcgt cgtagtcata ctcgagcccg gcggtgaaga tcgacgtgtc
     1321 ggcgaagaac cgggtcgtcg taccatgctc gtcgggagcg ctggtgccca ccaccttgac
     1381 cggcgcatct ggcacgcctc gcttgtagct ctgcttgtac accttgccgc cgcgccgaac
     1441 ttcgacttcc atccattcag acagcgcgtt cacgacagac gcgccgacgc cgtggaggcc
     1501 gccggaaacc ttgtatccgc cgccgccgaa cttaccgccg gcgtgcagga ccgtcatgat
     1561 gacttccagc gctgacttgt tcatcttcga gtgcttgtca accggaatgc cgcgcccgtt
     1621 gtcgaccacg gtcacgacgc catcggtgct gatggtggtc acgatgtggt cgcagtagcc
     1681 gccgagtgcc tcgtcgaccg agttgtcgac gatctcccgg accaggtgat gcaggccgcg
     1741 gtgatcggtg ctgccgatgt acatgcccgg tcgccgccgg accgcttcaa gaccctcgag
     1801 aatctgaatc gagctggcat cgtaatcagt ctttgtcttt gccctggtag tagccaagtg
     1861 cttcccccta aatcgtttgc tgtaacggag cgacgccatc gaagcgggcc cgataaaagg
     1921 cgaggctggc gagcgagaga ctcgttacca tcgccgcgtt gaggacgatt gcgatgccca
     1981 acccgacggg atcaccggcg attcgctccc aaatcgagag caggccgggc gagatcatca
     2041 gcgagaccgc gaaaaaggcg gcagattgcc aaaagaaccg gaacacgact tgcgcgcttg
     2101 cccgaacggc tgctatcggg ctcgcaccgt caatcacgat cgcttccgga gcgaacatgg
     2161 tgacaacgag cgcgatcgag ccaaccagcc aggcgagtcc ggccatcgtg gtggcactca
     2221 cgccgcctgc cgccaggatt gcggccggca cgatcatgac tgccgacaag gaggcgatca
     2281 cgccgatcag gagtccgaac agccccagca tcttgaccca tcggtcgagc agcaaacgca
     2341 tgggcggcaa tgaattcgcc cggatcatcc gtgttcgtga tgccagcatg gtgatgtaga
     2401 tggtgaagat caccgacccg atcagaatca tgccgatgct tgccgccacg acgccgaggg
     2461 atgaagggct ccaggccaac cggtcgaacg gccggtagta agtgtcttgc cccatcccat
     2521 cgagcacgct cggcatgagc agcgccagca gccgggagac gtcccagtcg cccaggtcgc
     2581 ggacccattt gccggcatcc agtgcgttct ggccgctccg ccccgcgaac cagtcgccga
     2641 gtcgcgatgt cagcgatccc gcggagatcc gcgcgccgaa gagcaaatag aggtcgagca
     2701 gtaccgggat aaggaccagc tgcgggtacg cgagcacatc ggacatgccg tcggacacgt
     2761 gttcgagtat cccgggcggt cgattcggct gtgagaccgt ggttcgctcc accatcaggc
     2821 caatccaagt tgatcgagct gctcctcatc gaacccaaga tggtgcccga tttcatggag
     2881 gagcgtgatt cgtatttgct cttcgagttc ccgcgggtcg ggaaacgcct cttcaagcgg
     2941 cttttggaag atcacaattc ggtcgggaac cgcgaaagtg tacccacttc cgcgttccgt
     3001 cttcgccaca ccgtcgtaca cgccgaattg ttccgggtcc ccatccggag gagcggcctc
     3061 gacgaccaca acgaggttgt cgagcgccag gcgaacagct ggatcaagct gagtcaggat
     3121 ccgatcaacg agcttttcga atcgaaggcg acgggatcgc ttgagcgttg aggcggctgt
     3181 ccgcatgggt agaattgtac cacttttgcg gatattttgc ctgcgttgac aagcgggaaa
     3241 cgctgcgtac gtatgcggct ttacgggcca acggcggcgg ccagaagctc gcgcccgata
     3301 tcaagcgcca ggcccccgcc ttcgccaccg taatcgaggc agaccgagac cacgattgcg
     3361 ggcgcatcga ctcccgcaga gccaatgaac cacccatgag gcgcttcccg gccggactct
     3421 gcggtgccgg tcttgccggc cacgttgatg cctgcaatcg cggcccggcg cgcgtatcca
     3481 tatccgacgc tctcgtagag catggagagg accgtatcgg ccgtttgact gctgattggc
     3541 cgccgccaga tcgagggctc ggttcgggtg ctgatgctgc cgtccgggtt atggacagcg
     3601 tcgaccaaaa tcggcttc
//
LOCUS       DASSGX010000431         3097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_182399, whole genome shotgun sequence.
ACCESSION   DASSGX010000431 DASSGX010000000
VERSION     DASSGX010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182399"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..330)
                     /locus_tag="VFI12_07640"
                     /pseudo
     CDS             complement(28..330)
                     /locus_tag="VFI12_07640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
     gene            complement(432..2126)
                     /gene="recN"
                     /locus_tag="VFI12_07645"
     CDS             complement(432..2126)
                     /gene="recN"
                     /locus_tag="VFI12_07645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HET7056321.1"
                     /translation="MLAELAIRNFAIIDELQLQFDDGMNALTGETGAGKSIIIDALGA
                     VLGERVGSEMVRTGASSATAEAVFDLTDRDITGIAALLDELGIEDEDGTLILSRQISA
                     GGRSTARVNGRPVTVGALNQVGSLLIDIHGQSDHLKLLRTADQLDALDHFAGTMSDRE
                     RIRELVHELHAVRAARGRLRTGEREREQRIDLLRYQVDEIAAAALEPNEDERIANERS
                     VLANAERLVEDASSALQAIEPDDEGGASSMLRTALQHVQRIAEVDTSTAELVERVNEV
                     VVLADDVASDLRRYVESVDVDPGRLAAVEERFDLIQKLRRKYGATIAEIQEHLAHAEA
                     ELETLTGAGFDDAALAAQEEQLERQLTPVLDALTTARRTAGERLERAIDAAIGELGLG
                     SAHVAITIEPLAEPTETGADAVEFLFAPNKGEALKSLAKIASGGETARLMLALKSVLA
                     EGDRTPTLVFDEVDVGVGARSGQAVGEKLWSLADRHQVIVISHLAQIAAFAGAHFMIE
                     KNVTDGRTISTVRRLEGGTRLDELAEMIDGKPVSPESRAAAEQLMDRISTVKRTAG"
     gene            complement(2130..>3097)
                     /locus_tag="VFI12_07650"
     CDS             complement(2130..>3097)
                     /locus_tag="VFI12_07650"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HET7056322.1"
                     /translation="MQTAPAPTSLTGAYGGEKWSPRNASFREDMIETWGDWGSGSECG
                     TLRAVLLRRPGPELDAIEDFDAVQMRADLNADLARAQHDVMAAAYESHGVTVHRVENG
                     RMDKPNSFFLRDLMLMTPEGAIITRPASTVRAGEERLVAEALGRIGVPILMSVHGSGT
                     FEGADVSWVNEDLCFLAEGLRTNEEGANQVERMLREIGVKDVIRVGLPYGAMHLDGLL
                     NFPDRDCAVVWARRTPYKIVDTLKRRGFRIVEVPDEVEAQNCLPMNFVALAPGEILMP
                     TGGDQMRRLYEAAGIICHAIEIGELIKAGGGIHCMTAFLKRDPLV"
BASE COUNT          493 a         1038 c          966 g          600 t
ORIGIN      
        1 acagaccgag gttacgacgg caggccgcta catgcccggg atcttgagac cgccggtgag
       61 ggcgcccatg cgttcctcca tcatcgcagc tgccttggtc atcgcgtcgg tgatggcggc
      121 gttgatcatg tcctcgagca actcgacatc gtccggatcg acagcagacg ggtcgatctt
      181 gatcgactgg aactccttct gtccgttgac cttggcgacg acgacgccac cgcctgcgct
      241 accctcgacg atcgtctcgc ccagctcctt ctggatcttc tccagtcgca tctgcatctg
      301 ctggatagcc ttcatgttga tgcccatggc gtcacgtgtt cctttcgcgt tgggatgatg
      361 tgggttgggt cttcctcaga caacgatggc tgagtatagg cgagcgactg ggcgatctgc
      421 gcactagcgg atcaccctgc ggtacgcttt accgtcgaga ttcggtccat gagctgctcg
      481 gccgcggcgc gagattccgg cgagaccggc ttgccatcga tcatctcggc taattcatcg
      541 agccgggttc caccttcaag ccggcgcacg gtcgagatcg tccggccgtc ggtcacattc
      601 ttctcgatca tgaagtgcgc cccggcaaat gcggcgatct gcgcaagatg gctgatcacg
      661 atcacctgat ggcgatcggc aagcgaccag agcttttccc cgaccgcctg tcccgaccgc
      721 gccccgacac caacatcgac ctcgtcgaag accagagtcg gcgtgcgatc gccctcggcc
      781 agcaccgatt tgagcgcgag catgagccgc gccgtctcac cgcccgatgc gattttggcc
      841 agcgacttga gcgcttcgcc cttgttcggg gcgaaaagga actcaaccgc gtcggccccg
      901 gtttcggtcg gctccgccag cggctcgatt gtgatggcga catgcgctga gccaagcccg
      961 agctcaccga tggcggcgtc gattgcgcgc tccaggcgtt cgccggctgt ccgccgggca
     1021 gtcgtgaggg cgtccagcac gggtgttagc tgccgctcaa gctgttcttc ctgcgcggcg
     1081 agggctgcat cgtcgaaccc ggcgccagtc agcgtctcca gttcggcttc ggcatgggcg
     1141 aggtgctcct ggatctcggc gatggtcgcg ccgtacttgc gcctgagctt ctggatcaga
     1201 tcgaatcgct cctcgacggc ggcgagccgg cccggatcga cgtcgactga ttcgacatac
     1261 cgccgcaggt ctgaggcgac gtcatcggca agcacgacca cttcattgac ccgctccacc
     1321 agctcagcag ttgacgtgtc gacctcggcg atgcgctgga catgttgcag cgcggtccgg
     1381 agcatgctcg atgcgccacc ttcgtcatcg ggctcgatcg cttgcaacgc ggacgacgcg
     1441 tcctcgacca ggcgctcggc gttggccaga accgaccgct cgttggcgat ccgctcgtcc
     1501 tcattgggtt ccagtgcggc ggcggcgatc tcatccacct ggtaccgcaa caggtcgatc
     1561 cgttgctcgc gttcgcgctc gccggtgcgc agccggcccc gggccgcgcg gactgcgtgc
     1621 agctcgtgga caagctcgcg gatgcgttcc cggtcgctca tcgtgccggc gaaatgatcg
     1681 agcgcatcga gctggtcggc agttcggagc agcttgagat ggtcggattg cccgtggatg
     1741 tcgatcagca acgagccgac ctggttgagc gcgccgacgg tcaccggccg cccattgacc
     1801 cgcgcggtcg atcggccgcc agccgagatc tgccgagaca gaatcagcgt gccgtcctca
     1861 tcctcgatac cgagttcgtc gagcagagct gcgatgcccg tgatgtcccg gtcggtgagg
     1921 tcgaagaccg cctctgcggt cgccgacgat gcgccggtgc gcaccatttc cgagccgact
     1981 cgctcgccga gcactgcacc gagggcgtcg atgatgatcg attttccggc gccggtttca
     2041 cccgtcagcg cgttcatgcc gtcatcgaac tgcagctgca gctcgtcgat gattgcgaag
     2101 ttgcggatag cgagttcggc cagcatgccc tagacgagcg gatctcgctt caggaaggcg
     2161 gtcatgcagt ggatgccgcc accggctttg atcagctcgc cgatctcgat cgcgtggcag
     2221 atgatcccgg ccgcttcgta gagccgtcgc atctggtcgc cgccggtcgg catcaggatt
     2281 tcacccggag cgagcgcgac gaagttcatc ggcaggcagt tttgcgcttc gacctcgtcc
     2341 ggcacctcga cgatgcggaa tccgcgccgc ttgagcgtat cgacgatctt gtacggcgtc
     2401 cttctggccc agacgactgc gcaatcccgg tccggaaagt tgagcaagcc gtccaggtgc
     2461 attgctccgt agggcaatcc gacccggatg acgtccttga ccccgatttc gcggagcatc
     2521 cgctcgacct ggttcgcacc ctcttcgttc gtgcgcagcc cctcggccag gaagcaaaga
     2581 tcctcgttca cccacgagac gtccgcgccc tcgaaggtgc cggagccgtg gacggacatc
     2641 aggattggaa caccgatccg gccgagcgcc tcggcgacca gtcgctcctc accggcccgg
     2701 acggtcgatg ccggccgggt tatgatcgcg ccttccggtg tcatcagcat caggtcgcgc
     2761 aggaagaacg agttcggttt atccatccgc ccattctcga cgcggtggac cgtgacgcca
     2821 tgcgactcgt acgccgctgc catcacatca tgctgcgctc gcgcgaggtc agcgttgagg
     2881 tccgcccgca tctggaccgc gtcgaagtct tcgatcgcgt ccagttccgg accgggtcgg
     2941 cgcaagagca cggcacgcag ggtgccgcac tccgagcccg agccccagtc gccccaggtc
     3001 tcgatcatgt cctcgcggaa cgacgcgttt cggggcgacc atttctcgcc gccatacgcg
     3061 ccggtgaggg atgtcggtgc cggtgccgtc tgcatct
//
LOCUS       DASSGX010000432         8526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_75069, whole genome shotgun sequence.
ACCESSION   DASSGX010000432 DASSGX010000000
VERSION     DASSGX010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 8526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8526
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75069"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..978)
                     /locus_tag="VFI12_07655"
     CDS             complement(160..978)
                     /locus_tag="VFI12_07655"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7056323.1"
                     /translation="MSTAIETHYSSDGHVADRIRAWVLASGKVIDQLVPADLAPVDEF
                     HIRGRPATIELADRMDLRADSRVLDLGSGLGGPARTLAERSGCHVTGIDLTREFCEAA
                     TILSGWVGLGDRVRFVQGDATALPFASASFDAALTIHAAMNIPAKDRLYAEAKRVLRP
                     GAIFAIYDVLQGEGGPVVYPVPWAREPATSALATPAEMRQLLGEAGFTLLEEIDSTDA
                     CRVWYRDRVAQAERVAQPVRFQLFLGDEYAAMARNQVQNLTEKRIRIGTYICRA"
     gene            complement(1093..1329)
                     /locus_tag="VFI12_07660"
     CDS             complement(1093..1329)
                     /locus_tag="VFI12_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056324.1"
                     /translation="MSDARDPAVFRLALNRALGDAGITVGIRPITPTNVWVVAWRVNG
                     KSVMRILHVALDGETPDLVEQVAALIRREFRKYP"
     gene            complement(1708..2130)
                     /locus_tag="VFI12_07665"
     CDS             complement(1708..2130)
                     /locus_tag="VFI12_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HET7056325.1"
                     /translation="MSLSSFPSVPRRIVSDPQVLGGRWHVADTTVAVGEVRLDHATNG
                     CHAGYTYPSLSRAELAACLVFDFPPTRESGVAMLAGTVVVSCACGEDISAAGGLDEPV
                     HCVCGRVWRLRLLLNLVQDGGIAVRDTAVGAVFLPAAP"
     gene            complement(2466..2738)
                     /locus_tag="VFI12_07670"
     CDS             complement(2466..2738)
                     /locus_tag="VFI12_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056326.1"
                     /translation="MATLHADVRDTDRGERRVEVRNAYTETKGSLLTTEFWAMAGVIA
                     AILVAAQRLDHFSGQEAWTLVAAVAIGYMLSRGLAKAGSGYRDSDR"
     gene            2998..3666
                     /locus_tag="VFI12_07675"
     CDS             2998..3666
                     /locus_tag="VFI12_07675"
                     /inference="COORDINATES: protein motif:HMM:NF019544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET7056327.1"
                     /translation="MARGTVIVLNGTSSAGKSSLARAIQDVMDEPYLVLAMDVFLEHF
                     PERYLGDSGPDRVPTPVERAGIVWACTEPGGRGFRELEIGPWVKALWSGLSHAAAAAR
                     RGVNVVLDDVILDPAMRADYRAALSGVPTLMVRVCCPPDVLTAREQARGDRNAGMALA
                     MLPLVDANAPPSDLTIDTNLVTPEEAAYAIKAALADRQRVPTDRGAGYEDPPGDRSLP
                     AAAP"
     gene            complement(3722..4186)
                     /locus_tag="VFI12_07680"
     CDS             complement(3722..4186)
                     /locus_tag="VFI12_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056328.1"
                     /translation="MQMLQIRRSVVMLLIALCTIAAGPNRAGAQPGTQSALVAVTGQG
                     TGRVLIAPNRADQGTFAAQITVTVHGAAPGTTFTVARAADLVADGVCTGPTFAAFPGA
                     ILTTSGGGAGVTHIDFHRGAPFVSGISFDVVFQLAGSDGSLLESDCMTVTVL"
     gene            complement(4224..5324)
                     /locus_tag="VFI12_07685"
     CDS             complement(4224..5324)
                     /locus_tag="VFI12_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056329.1"
                     /translation="MATDPTERRLRHADTPIAPRPEFAATLLTRLLAELEADRPPTDD
                     AESGYPRAPQASQRLRSEVRAAARAVPAGTNSPVTVPRTRRGSALVAIAAVLLVAIGL
                     GARMLLPTGPDRPPAIPAMSFPSPTALGLGEILLRIDVAAWNPSTEPATPGPDGGSPQ
                     GRRIDLFRIMIEPDAPYAEYTRDLGTRLILVESGAIEVTLDGPGQLLRAGAPNADDVG
                     AGPPVTLEPGDALVTPGGTHRTLRAIDSKAATALQLIEHRWIQKDLGVVASGPQPEML
                     ARGALVVTIARGELAPNGVLPAPRAGAVAIVEVEAGTVGVERTDGGHARSFTYSRGGD
                     VVVGADPAGITELRNIGTEPARLLVVLFEPAN"
     gene            complement(5314..5862)
                     /locus_tag="VFI12_07690"
     CDS             complement(5314..5862)
                     /locus_tag="VFI12_07690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET7056330.1"
                     /translation="MVADIAAVEVSGWDLTDPDAFRAFYADALPRVYGYFFVRCGAEA
                     SVAEDLTQETFLAVVAELNRGRPVAAPLPWVVGIARHKLIDHLRRQRRAGWSVIAWEE
                     AAEEQTPLAESEEVSSARAIAALAAVPSPQREALALRHLDGLSVPEVAAALGRSVAAA
                     ESLLARGRVTFRRVYAEASDGD"
     gene            complement(6275..6784)
                     /locus_tag="VFI12_07695"
     CDS             complement(6275..6784)
                     /locus_tag="VFI12_07695"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET7056331.1"
                     /translation="MVHPLVEQLRFARIEFRRCLEGVSADDAERRLLPMNSISWIICH
                     LAGQERRFWLIWAQGRTDVAPELDEWGHIDKPANTPPLAGAWASWETVRAAVDPYLDA
                     LTQVRLLERLDVNGAPMESNIGTMLRRSTYHYWYHTGEANAIRQLLRHQNVPELVGEI
                     QKEAPYRPE"
     gene            complement(6820..7095)
                     /locus_tag="VFI12_07700"
     CDS             complement(6820..7095)
                     /locus_tag="VFI12_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056332.1"
                     /translation="MSERSEELIPTASVGYTLAMFAEEVQALLRPAGIEARLIEAEDG
                     ARSIRAWPRTAPHDSALFAIDDVRISPYQAHIVGSVIRRSFRLVTSD"
     gene            complement(7261..7458)
                     /locus_tag="VFI12_07705"
     CDS             complement(7261..7458)
                     /locus_tag="VFI12_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056333.1"
                     /translation="MFEMTPTAMTVRTMRFQDDVAQAERYHALVRGDKVVGKTRVAFP
                     AIRLPRISPISRLFHTRLRPA"
     gene            complement(7630..>8526)
                     /locus_tag="VFI12_07710"
     CDS             complement(7630..>8526)
                     /locus_tag="VFI12_07710"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7056334.1"
                     /translation="LGDENREKMAVGWLGFIALAQGDYDRTDLLAHQAIAMDGQFNTG
                     WAVYFQWGYLGQAAHGKGDLISARTYYEEALAEEAVAEEQPGSDPFDRAIVLGYLALL
                     DCQEGAYPAAATRLSEALTIWRALNNQENSAECLAQVAVLANAIGDHEASVRFLAAGI
                     ALRDAVEHAWVLPERAAYERTEQSLRETLRPDAFARAYREGLDQPLDKMLDEASAFLA
                     LYCSGEDGAPAVRAADSFGLTPRERDVLRLLAQGKSDREIAEALFIGTRTVETHVSNL
                     IAKLGVHNRTEAATLATRDHLV"
BASE COUNT         1447 a         2802 c         2889 g         1388 t
ORIGIN      
        1 ggtcgggact aggtccagcc ggggatgcgc gcgacgatga gaactagcat ggcaatggcg
       61 agcacatctc aggagtgccg agggcggttg ggttccccaa gcgttatggc acgccctgtt
      121 cgctgcgacc ggcatttgca gagcatcgcg accgggggtt caggcccgac agatataggt
      181 cccgatccgg atccgcttct cggtcaggtt ctggacctgg ttgcgggcca tcgcggcgta
      241 ctcatcccca agaaacagct ggaagcgcac cggttgggca acccgctccg cctgagcaac
      301 ccggtcccgg taccacacgc ggcacgcgtc ggtggagtca atctcctcga ggagcgtaaa
      361 gccggcctcg cccaggagtt gccgcatctc ggctggggtg gcgagggcgc tggtcgccgg
      421 ctcccgcgcc cacgggacgg gatagaccac cggcccgccc tccccctgga gcacgtcgta
      481 gatggcgaag atggcgcctg gcctgagcac ccgcttggcc tccgcgtaaa ggcggtcctt
      541 ggcggggatg ttcatcgcgg catgaattgt cagggcagca tcgaagctgg cggaggcgaa
      601 cggcagcgcg gtcgcatcgc cctgaacgaa gcgcacccgg tcgcccaacc cgacccatcc
      661 ggacagaatc gtggccgctt cgcagaactc acgggtcagg tcgatcccgg tgacgtgaca
      721 gccggagcgc tccgccaggg tgcgcgccgg gcccccgagg ccgctgccca gatcgagcac
      781 gcggctgtcg gcgcgcaggt ccatgcggtc ggccagttca atggtggccg gccggccccg
      841 gatgtgaaac tcgtccacgg gcgccagatc ggccggcacg agctggtcga tcactttgcc
      901 ggaggcgagc acccaggctc ggatccggtc ggccacgtgc ccatcgctgc tgtagtgggt
      961 ctcgatcgcc gtcgacatag ggcgctcctg ggatccggtg gccgcgcaga tcggccgcta
     1021 tcgccggcgg gcgtggccgg tctcccgctg atactacata gtggacgacg gatgcgatcg
     1081 gagcagcggc cgtcagggat acttccgaaa ctcccgccga atcagcgcgg ccacctgctc
     1141 gaccagatca ggcgtctccc cgtccagggc gacatgcagg atccgcatga ccgatttgcc
     1201 gttcacccgc cacgcgacaa cccagacgtt ggtcggagtg atcggccgga tcccgaccgt
     1261 gatcccggcg tcgcccaggg cacggttgag cgccagtcga aacaccgccg ggtcccgcgc
     1321 gtcactcatc gtggctcgat acgtcagtgc tgctgcgaaa tgctgctggg atggtaccgc
     1381 gatttatcac gccgtgtggc cgccgcttac ccggaccctg actcgggcag cgagggatcc
     1441 ggggcgtcgg ggctcgggaa ctcctcgacg agctccacct ggagctgcca gcgccgccag
     1501 cagaggggac agacgatcgg atcggtggcc tcggccacgg caatggtgcc gccacacggg
     1561 catcgcagga gtacgggccc ggtaaagcgg gcagagggtc ggcggatggg atcgggaggc
     1621 atgagggagt atccgaacaa tgcacgaggg gcaggggcac acgccgcgcc gccctcgatt
     1681 atgggccagt aggtccgggt ggagtcgtta gggagctgca ggcaggaaga cggcgccgac
     1741 agcggtgtcc cggaccgcta tgccgccgtc ctggaccaga ttgagtagca ggcgcaagcg
     1801 ccagacccgg ccacagacgc agtggaccgg ctcgtcaagt ccgcccgcgg cgctgatgtc
     1861 ctcaccacag gcgcaggaga cgacgacggt tccggcgagc atggcgacgc cggattcccg
     1921 cgtcggcgga aagtcgaaga ccaggcaggc ggccagctcc gcgcgggaga ggctggggta
     1981 ggtatagcct gcgtggcacc cgttcgtggc atggtcgaga cgcacctcgc ccacggcgac
     2041 cgtggtgtcc gcgacgtgcc aacggccacc cagaacctgg ggatccgaga cgatgcgacg
     2101 cggaactgaa ggaaaagaag agagcgacat acgtgactcc ttgggtgagc aggcatccag
     2161 cagggggagc gctcgtgggc taccaagggc ggcagtcata tttccccggg agagacccgc
     2221 aagggaaccg ggaagaagtc aagcgcgctt agcttagcat aataaccaaa tgtacgcgag
     2281 tgtgggtgcg cacgcgcggc taccggccgc cgctcgcttc gccccaccgc gagggagcag
     2341 gttagcttgg cgatggcgtc ggtccccgtc atccgccggt gccatgcgac cgacccgggg
     2401 cactactgcc ccgggtcggt gccgtgctgg tgtcaatggc tccgcggcac ggggccgcgg
     2461 accggctagc ggtcggagtc gcggtagccc gagcccgcct tggccaggcc ccggctgagc
     2521 atgtagccga tcgcaacggc ggccacaagc gtccaggcct cctgcccgct gaaatgatcg
     2581 agccgctgcg cggcgaccag gatcgccgca atcaccccgg ccatcgccca gaactcggtg
     2641 gtcaggagcg agcccttggt ctcggtatag gcgttgcgca cctcgacccg gcgctcgccg
     2701 cggtcggtat cgcggacatc ggcatgtaaa gtggccacgt ggggtactcc tcgtgatagt
     2761 cgctgactgc gcttgaggga tttccccgaa cctaccaagt atcaggggag agggccgaga
     2821 ccagctgtgc ggatgctacg ccacagaaag gttgtacggg ccagggcgcc acgcacgcgc
     2881 atcggccggc caggaaacga gcgatgctct ggcgcctatt ccgtgggctt acgccgccaa
     2941 tgtaggatcg aaagcgtttc gcaatgcgaa caggtcgcct cgggcgggag caagacaatg
     3001 gcgcgtggca cggtgattgt gctcaatggc acctcgagtg cggggaagag ctcgctcgca
     3061 cgcgccatcc aggacgtcat ggatgagccc tacctggtgc tagcgatgga cgtcttcctt
     3121 gagcatttcc cggaacgata cctgggcgat agcggcccgg atcgcgtgcc gacgcctgtc
     3181 gagcgcgccg gcatcgtctg ggcgtgcacc gagccgggcg ggcgcggctt tcgcgagctc
     3241 gagatcgggc cgtgggttaa ggccttatgg tcaggcctga gccatgccgc ggcggcggcc
     3301 cggcggggcg tcaatgttgt gcttgatgac gtgatcctcg atccagcgat gcgcgccgat
     3361 taccgcgccg ccctttccgg cgttccgacg ctcatggtcc gggtgtgctg tccgccggac
     3421 gtgctcacgg cacgtgagca ggcgcgggga gatcggaatg cgggcatggc cctcgccatg
     3481 cttcccctcg tcgacgccaa cgcgccgcct tccgacctaa ccatcgatac caaccttgtg
     3541 acccctgagg aagcggccta cgccatcaag gcggcactcg ccgaccggca gcgagtgcct
     3601 accgatcgtg gagccggtta cgaggatccg cccggggacc gctcgctacc tgccgcggcg
     3661 ccctgactaa ctagcccggt cgttgcgggc aatgcacatt gggcgctggc cgctggcgtg
     3721 actacagcac agtgacggtc atgcagtcgc tctccagcag cgatccatcg ctgcccgcca
     3781 gttggaagac gacatcaaag gagatgccgg agacgaacgg ggccccccgg tggaaatcaa
     3841 tgtgggtcac cccggccccg ccaccagagg tcgtcaggat ggctcccggg aaggccgcga
     3901 aggtcgggcc cgtgcagacg ccgtccgcga ccagatccgc cgcacgcgcg acggtgaaag
     3961 tcgtgccggg cgcggcgccg tggacggtca ccgtgatttg ggccgcgaag gtcccctgat
     4021 ccgctcggtt gggagcgatg aggacccgcc ccgttccctg gccggtcacc gctaccaggg
     4081 cactctgggt gcctggttgc gccccggctc tgtttggtcc tgccgcgatc gtacagaggg
     4141 cgatgagaag catgacgaca gaccgtcgaa tctgcagcat ttgcattggg atcctcctga
     4201 tcggcaatct cttttcccag acatcaattg gccggttcga acagaacgac gaggagccga
     4261 gccggctcgg tgccgatgtt gcggagctca gtaatacctg cggggtccgc acccacgaca
     4321 acatcaccgc cacggctgta tgtgaatgag cgagcatggc ctccgtccgt gcgctctact
     4381 cccacggtgc ccgcttccac ttcgacaatc gcgacggccc cggcgcgagg agcaggcagg
     4441 actccgtttg gggcgagctc gccccgggcg atagtaacga cgagcgcccc gcgggccagc
     4501 atctccggct ggggtcccga cgccacaacg ccaaggtctt tttggatcca ccgatgctcg
     4561 atcaattgca gcgccgtggc tgctttcgaa tcgatggcac ggagcgtgcg gtgggtcccg
     4621 cccggcgtca ccagcgcgtc gcccggctcg agcgtcaccg gcggcccagc ccccacgtca
     4681 tctgcattgg gcgctcccgc tcgcaacagt tgaccgggac cgtcgagggt aacctcgatc
     4741 gcgccggact caaccaggat caggcgggtg ccgagatctc gggtgtactc cgcgtacgga
     4801 gcgtccggct caatcatgat ccggaacagg tcaatccggc gcccctgagg gcttcccccg
     4861 tctggcccgg gtgtggcagg ctcggttgaa gggttccacg cggccacatc aatgcgaagc
     4921 aagatctcgc caagacccaa ggcggtgggc gatgggaagc tcatagcggg aatggcggga
     4981 gggcgatccg gaccggtcgg gaggagcatg cgggcaccca gcccgatcgc gacgagcagg
     5041 actgccgcga tcgccaccag cgcgcttccc cgccgcgtcc gcgggaccgt gaccgggctg
     5101 tttgtgcccg ccggcaccgc tcgggccgct gcgcgcacct cggaacgaag ccgctggctc
     5161 gcttgcgggg cacgggggta gccagactcc gcgtcgtccg tcggtggacg atcggcctcc
     5221 agctcggcaa gcaggcgcgt tagcaacgtc gcggcgaact cgggacgcgg cgcgatcggg
     5281 gtgtcggcgt gccggagccg tcgctcggtc gggtcagtcg ccatcgctgg cctccgcata
     5341 cacccgacgg aaggtcaccc gaccgcgggc gagcagcgat tcggcggcgg ccacgctccg
     5401 gcccagggcg gccgccacct cgggcacgga caacccatca aggtgtcgca atgccagcgc
     5461 ttcccgctgc ggcgacggca cggcggcgag ggccgcgatc gcccgtgcac tactgacctc
     5521 ctcgctctcc gcgagtggcg tctgctcctc cgccgcctcc tcccatgcga tcacggacca
     5581 gccggcccgg cgctgccgcc gcaggtgatc gatcagcttg tggcgagcaa tgccaaccac
     5641 ccagggcaac ggtgccgcga cggggcgccc ccggttgagt tcggccacca cagccagaaa
     5701 tgtctcctgg gtgaggtctt cggcgacgct tgcctccgcc ccgcaccgga cgaagaagta
     5761 gccatagacg cgcggcaggg cgtccgcata gaacgcgcgg aaggcatcgg gatccgtgag
     5821 gtcccatccg ctcacctcta ctgccgcgat gtcggccacc atctccccct gcgcgaggtt
     5881 cgctcgcttg ccaaaatagt cgcagggcgg gaccgttttc cgtcgcgggg acgccgatgg
     5941 gatgcgaacc ggccgcggac acctggcgcg atgtagccca ccgggctacc aagggccagt
     6001 cgttcattat ttgcgggcaa ttcaccttat ccctataaat cggcccggct cgaccgggtc
     6061 aatatggccc gctctcgcgt gaggcgtcga atttcgcgag aatcggccaa tctccggcgc
     6121 gtctggccgg tttgcggaat tgggcccgtt tgcccgttgc acgcgtcctg gggcttctag
     6181 acgcgatagc ctgttcgaat ctcaggccgg ctggcgggtt actggcgttc caccgacgac
     6241 cgccgcatcg gcggtcgtcc cgaatcggtc gggttcattc cggccggtag ggggcctctt
     6301 tctggatctc gccgacgagc tccggcacgt tctgatgcct cagcaattgg cgaatcgcgt
     6361 tcgcttcccc cgtgtgatac cagtagtgat aggttgagcg ccgcagcatg gtgccgatgt
     6421 tcgactccat cggcgcgccg ttgacgtcga gccgctccag caatcgcacc tgggtgagcg
     6481 cgtcgagata gggatcaacg gccgcccgga cggtctccca ggaggcccag gccccggcca
     6541 gtggcggcgt gttggccggc ttgtcgatgt ggccccattc gtccagctcc ggggcaacat
     6601 cggttctacc ctgcgcccag atcagccaaa agcggcgctc ctgcccggcc agatggcaga
     6661 tgatccagct aatgctgttc atcggcagga ggcggcgctc ggcatcgtcg gcagacacgc
     6721 cctccaggca gcggcgaaac tcgatgcggg caaagcggag ttgctcgacc agcggatgaa
     6781 ccatgatatt gcccttcgcc cacggttggt gaaccccgtc tagtcgcttg tcaccagccg
     6841 gaagctacgc cggatcacgc tgccgacaat atgggcctga tagggcgaga tccggacatc
     6901 gtcgatggca aagagagcgc tgtcgtgggg tgccgtgcgc ggccacgccc gaatcgagcg
     6961 cgcgccatcc tccgcctcta tgaggcgtgc ttcaatgccg gcggggcgca acagcgcctg
     7021 cacctcctcg gcaaacatgg ccagcgtgta gccgacgctg gcggtcggga tcagctcttc
     7081 gctgcgctcg ctcatggtcc ttcccctccc aggtgaagat cccatcatag ggccgctggc
     7141 aagtgcccgg ctcggtcccc ctgcaggatc ggaagtttag gggcgccggc agcgcaatcg
     7201 cccgtatgaa aaccacccgg gcggctgctt tgggttcggg gtggggacgg gtggcacgga
     7261 ttaggcgggg cggagccgcg tgtggaaaag gcggctgatc ggggagatgc gcggcaatcg
     7321 aatcgcgggg aacgcaactc gtgtcttgcc gacaaccttg tcgccgcgaa ccagcgcgtg
     7381 atagcgctct gcttgggcca cgtcatcttg gaaccgcatg gtgcggacgg tcatcgcggt
     7441 gggcgtcatt tcgaacatct cggaatcctt ccccggtgat ttttgtccgg ccagttcctc
     7501 cccggccatg caggcatcat ggcggtgcgg aaactgcgga acatcgggga atccacggaa
     7561 gcggggtacg gaattgctgg cgcgcagaat cggcgtgtct ccgtacattc ccggagggga
     7621 tggccggcgt cagaccagat ggtcgcgggt ggcgagggtg gcggcctcgg tacggttgtg
     7681 gacaccaagt ttggcaatga ggttagagac gtgggtttcg accgtacggg taccgatgaa
     7741 gagcgcctcg gcgatctccc ggtctgactt gccctgggcg aggagacgga ggacgtcgcg
     7801 ctcgcgagga gtcaggccaa atgaatcagc ggcccggacc gctggcgcgc catcctcccc
     7861 tgaacagtac agcgctagga atgctgaggc ctcgtcgagc atcttgtcca gcggctgatc
     7921 gagtccctcc cggtaggcac gggcgaaggc gtcgggacgg agcgtctcac gcagtgactg
     7981 ctctgtgcgc tcgtacgccg ctcgctccgg caacacccag gcatgttcca cggcgtcccg
     8041 cagtgcaatt ccggcagcca ggaagcggac gcttgcttca tggtcgccaa tcgcgttggc
     8101 aaggaccgca acctgcgcca ggcactccgc actattctcc tgattgttga gcgcccgcca
     8161 gatggtcagc gcttccgaca ggcgcgtggc ggccgcgggg tacgcgcctt cttgacaatc
     8221 gaggagggcg agatacccca gtacgattgc cctatcaaac ggatcagaac ctggctgctc
     8281 ttcggcgacc gcctcttccg cgagcgcctc ttcatagtag gtgcgtgctg atatgaggtc
     8341 acccttgcca tgcgccgcct gcccaaggta tccccactga aagtaaacgg cccaacccgt
     8401 gttgaattgc ccatccatgg caattgcctg gtgagcaagg aggtcggtgc ggtcgtagtc
     8461 tccttgcgcg agcgcgataa acccgagcca gcccacggcc attttctcgc gattctcgtc
     8521 tcccag
//
LOCUS       DASSGX010000433         2983 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1054169, whole genome shotgun sequence.
ACCESSION   DASSGX010000433 DASSGX010000000
VERSION     DASSGX010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054169"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            506..1276
                     /locus_tag="VFI12_07715"
     CDS             506..1276
                     /locus_tag="VFI12_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HET7056335.1"
                     /translation="MLWDASAINGYAIEASDGQLGTVSDLLFEDVGWIVRWLVVDTGN
                     WLPGRKVLLPLSALGHPDRALRHFPIKLTMQQVKDSPDVDMDLPVSRQIEAQVHDYFG
                     WDPYWSGSFPAMSNAMATPFVAPLYGQQSIPNDLADTDAQTNEGDPDLRSMAAITGCH
                     IHASDGEIGHVEDFLVDDAGWNIRYIKVDTRNWWPGQQVLISPLSVGEIDWAEKLLQI
                     NVNRQQIKDAPPYDPSITVDGAYEEKFLTYYGIRWVAA"
     gene            1309..>2983
                     /locus_tag="VFI12_07720"
     CDS             1309..>2983
                     /locus_tag="VFI12_07720"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7056336.1"
                     /translation="MSEEKKKLSGPDLTQGVELSTIPDGTMLLGHAQGEPVLLARRGD
                     EVFAIGAICTHYGAPLEQGLLDGYTVRCQWHHACFSLRTGEALRAPALNPVSHWRVEH
                     WHREEVRDVARQFTPVETPVGSVCVQEKSGGTVYVREKLESVNRPAQPLTGGIPATVV
                     IVGGGAAGNAAAEMLRREGYSGRITMLSADESVPCDRPNLSKGFLSGAVSDESNPLRS
                     EKFYKDHEIDLRLGTRVTAIDTAARQVRLADGSRHPYEALLLATGAEPVRLDIPGATL
                     RHVHYLRTLADGRALVASALAAKRAVVIGASFIGLEVAASLRARNIEVHVVGTEAVLM
                     EKVLGPEVGNFLRKLHEDHGVTFHLGTTATTIDKQSVMLKSGERLQADLVVVGIGVRL
                     AISLAEQAGLAIDRGVVVNEYLESSIRGIFAVGDIARWPDRLTGERIRVEHWVVAERQ
                     GQTAAHNILGRRERFDYVPFFWTEQYDFGLAYVGHAEHWDKAEIAGSLEDRDCAITYR
                     RGGQKRAVAVVHRDLEGLRAEVEFERVIARDSAGGRTVKGTAPPVLHAQT"
BASE COUNT          568 a          895 c          982 g          538 t
ORIGIN      
        1 ggaagcggcg atcaaaaaag actgagggcc cccggctgcg cgggtcgtac caggcgtccg
       61 aggttccggc gaccagggac acgtgctcgg aggcgaccaa tgtcgtggtc gccatcacct
      121 gccccgcaag tccgaggagc gcccgtggac gtgcgcaatc ggggccgttc ggtcggggac
      181 aaacgaacct cggctgaccc cgcggaaagg catataggcg cgatgtccgt tgggctcgtt
      241 gcccaatgaa atgcgcgcat cggccaactg gcctcgccgc ggtcggatct gcgtagtttc
      301 cgagtctccc gccgttgccc tcgctcgcta caatcggtcc aatcagcacc gggagtgatc
      361 aacgccaagc tcgcacggac aggtggaagt tcgcccctgg cgatgtcccg gagtatccgt
      421 gccgcgcgcc aggccacgcg tggagaccgt gccgccgcgc atgtcggagc ttctggccga
      481 tcggcgaata ttcgggagta cggccatgtt gtgggacgca tcggcaatca atggctacgc
      541 gatcgaggcc agcgacggcc agctcgggac cgtcagtgat ttgctgtttg aagatgtcgg
      601 ctggatcgtt cgatggcttg tggtcgacac gggcaattgg cttcccggcc gcaaagtcct
      661 tctgccgcta tcggcgctag ggcatcccga ccgggcgttg cgtcacttcc cgatcaagct
      721 gaccatgcag caagtcaagg acagcccaga tgtcgacatg gacctgcctg tgtcacggca
      781 gatcgaagcg caggtccatg actatttcgg ttgggatccg tattggagcg gcagcttccc
      841 ggcgatgagt aacgccatgg ccacgccatt cgtcgcgccg ctctacgggc agcagtctat
      901 tccaaatgac ttggctgaca ccgatgctca aaccaatgag ggtgatccgg atctgcgcag
      961 catggcagct atcaccgggt gccatatcca cgccagcgat ggcgagatcg gtcatgtcga
     1021 ggattttctc gtggacgatg ctggctggaa catccgttac atcaaagtcg ataccaggaa
     1081 ctggtggccc ggacagcaag tgctgatctc gccgttgtcg gtaggagaga tcgattgggc
     1141 ggaaaaactt ttgcaaatca atgtcaaccg ccagcagata aaagatgcgc caccctacga
     1201 tccgtccatc actgtcgatg gagcctatga agaaaagttc ctgacgtatt acggcattag
     1261 atgggtcgct gcttaacgtc gcccagcgtc aacatccagg agattcgaat gagcgaggaa
     1321 aagaagaagc tcagcgggcc cgatctcact caaggcgtcg agctctcaac gattccagat
     1381 ggaaccatgc tgcttggcca tgcgcagggc gagccggtgc ttctcgcccg gcgcggcgat
     1441 gaggtgttcg cgatcggggc catctgcacg cactatggcg cgccgctcga acaggggctt
     1501 ctcgatggct atacggttcg gtgccaatgg catcatgcct gtttcagcct gcgcaccggc
     1561 gaggctctcc gtgcgccagc gctaaacccg gtttcgcact ggcgcgttga acattggcat
     1621 cgcgaagagg tgcgcgatgt ggcgcgtcag tttacgcccg tcgaaacgcc ggtcggctcc
     1681 gtgtgcgttc aagagaaatc tggcggcacc gtctatgtcc gggagaaact ggagagcgtc
     1741 aatcggcctg cacagccgtt gacgggcggc ataccggcga cggtcgtcat cgttggcggc
     1801 ggcgcggcgg gtaatgcggc cgccgagatg ctccgccgcg aaggctactc cggccgcatc
     1861 acgatgctga gcgcggatga atcagtgccg tgcgaccggc ccaacctgtc caagggcttc
     1921 ctctcaggcg cggtgtccga cgagtcgaat ccgctccgat cggagaaatt ctacaaggac
     1981 cacgaaatcg atctgcgcct tggcacgcgc gtgacggcca tcgacacggc ggcccggcag
     2041 gttcggctcg ccgatggcag ccggcacccc tatgaggcgc tcctgcttgc taccggggcc
     2101 gagccggtgc gtctcgacat ccccggggcc actctccggc acgtgcatta tcttcggacg
     2161 ctggctgacg gccgtgcgct ggtcgccagc gctctggcgg caaagcgcgc ggttgtgatc
     2221 ggcgccagct ttattggtct ggaggtggcg gcatcgcttc gtgcccggaa catcgaagtc
     2281 cacgtggtcg ggaccgaggc ggtcttgatg gagaaagtgc tgggaccgga ggtcgggaat
     2341 ttcctgcgca agctccatga ggatcatggc gtgacattcc acctcggcac gacggcgacg
     2401 acgatagaca agcaaagcgt gatgctgaaa agcggagaac gccttcaggc ggacctggtc
     2461 gtcgtcggca tcggcgtgcg gctagcaatc tcgctagcgg agcaagcagg gctcgcgatc
     2521 gatcgcggtg tcgtcgtcaa cgagtatctc gagagcagca tccggggcat cttcgccgtc
     2581 ggcgacatcg cgcgctggcc cgaccgactg accggcgagc ggattcgcgt cgaacactgg
     2641 gtcgtcgccg agcgccaggg gcagaccgcg gcgcacaaca tcctcggccg gcgcgaacgc
     2701 ttcgattacg tgccgttctt ttggacggaa cagtacgact tcggcttggc ctatgtcggt
     2761 cacgccgaac attgggacaa ggccgagatc gcggggtcgc tggaggatag agactgcgcc
     2821 ataacctatc ggcgcggagg ccagaagcgc gcggtcgccg tggtccatcg cgatcttgag
     2881 gggctccgcg cggaggtcga gttcgaacgg gtgatagcga gggacagcgc cggcggccgg
     2941 acggttaaag gcacggcgcc accggtgcta cacgctcaaa ctg
//
LOCUS       DASSGX010000434         2782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_510418, whole genome shotgun sequence.
ACCESSION   DASSGX010000434 DASSGX010000000
VERSION     DASSGX010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510418"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VFI12_07725"
     CDS             complement(<1..317)
                     /locus_tag="VFI12_07725"
                     /inference="COORDINATES: protein motif:HMM:NF015721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET7056337.1"
                     /translation="MGRDMDSGEHIAGQVDIAESAIAELVRDAVRTCYGVVGIGTSSS
                     RGLSHRLPRPWRRQPITISADAGRISVTIPIVVEYGTPITTVARNVIQTVAFHLRQSL
                     GLDV"
     gene            complement(349..1506)
                     /gene="queG"
                     /locus_tag="VFI12_07730"
     CDS             complement(349..1506)
                     /gene="queG"
                     /locus_tag="VFI12_07730"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HET7056338.1"
                     /translation="MTAGFPHATDLSATIKRLAHDCGLAVASISSADPFPGLAELLDD
                     RIAQAHLRGMDWFTPERSVVSANPRMLHERAQSIISVGIPYFRNDVEKPDDGVKRGRI
                     ARYAWGVDYHKTLKTRMAELAARIEALVGSSIEVRLLVDTARIVDRAVAARSGLGWYG
                     KHTNIIVPGHGSFVMLGEILIDLELESDQPLAKDCGRCRICLDRCPTGAIVAPYTVHA
                     PLCISYQTIENRGIIPADVRAKMGDWVFGCDVCQDVCPYTGAARSMYDEAFAPTSTEN
                     AFPSLHWLLRMTEDEFRATYRGTAVLRAKRAGLARNAAIALGNVGDADDAAFLSEALA
                     SHDQPLVRTHIAWALGQIGGAVACAALDAALRTDPDDEVQCECRAALERAA"
     gene            complement(1503..2444)
                     /locus_tag="VFI12_07735"
     CDS             complement(1503..2444)
                     /locus_tag="VFI12_07735"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HET7056339.1"
                     /translation="MSDTKPHPKITAIGVVSWDETLLIDRYPAEGDFAFILKSFNGPG
                     GTTGNTAMAARHLGADVSLHALVGCDERGDRILEALGEAGVDTGGCMRSLKVSDLSQM
                     LVSRETSERTIFWDQQPSIKRRDRIDIDALFNADVTVVDCVDYELRKFLSDLPAHTRP
                     AARLLGTLTYLADVVADDKLEVALRHDILVGNDREYRELLGVKSATDALESVAEAMPG
                     HNLRLAVMTRRHSGAMAVTRDRSLSTPAREVTVADTTGAGDAFAGALAFAVALRWDLE
                     RSLRFANAVASIVVTEFGAQTALPTYEEALAAAGLSP"
     gene            complement(2462..>2782)
                     /locus_tag="VFI12_07740"
     CDS             complement(2462..>2782)
                     /locus_tag="VFI12_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056340.1"
                     /translation="ALLIYIVGLLIGAGVSVQRMALTELFDVRPDRDRWLALAVSGVA
                     CLAPLLVGWTGNQVAKDWVLVGGIVLAMLVMAMLGTFPGGPARQRLAHTAPSPSGPAL
                     LPRR"
BASE COUNT          499 a          884 c          940 g          459 t
ORIGIN      
        1 acatcgagac ccaacgattg ccggaggtgg aaggcgacgg tctggatcac gttgcgggcc
       61 acggttgtga tcggcgtgcc atattcaacg acgatcggga tggtgacgga gatccggcca
      121 gcgtccgctg aaatggtgat cggctggcga cgccacggcc ggggaagacg gtgagatagc
      181 ccccgggacg agctcgtgcc gatcccgacg accccgtagc acgtccgcac cgcgtcccgc
      241 acgagctcgg cgatggcgct ttccgcgata tcgacttgtc ccgcgatgtg ttccccgctg
      301 tccatatcgc ggcccatgat atcgcgcccg gccggagcgt gacgcagatt acgccgctcg
      361 ttccagcgcc gcgcggcatt cgcattgcac ctcatcgtcc ggatctgttc gcagtgccgc
      421 gtccagtgcc gcgcaggcaa ccgctccgcc gatctgtccc aatgcccacg ctatgtgggt
      481 gcggacgagc ggctggtcgt gtgatgcgag ggcttcggag aggaaagccg cgtcgtcagc
      541 gtcgccgaca ttgccgagcg caatcgccgc gttgcgcgca aggccggcgc gtttggcccg
      601 gagcaccgcc gtgccccgat aggtcgcccg gaactcgtcc tcggtcatgc gcaggagcca
      661 gtgaagggat gggaacgcgt tctccgtgct cgtgggcgcg aacgcttcgt cgtacatgga
      721 gcgagccgcg ccggtgtacg ggcaaacatc ctggcagacg tcgcaaccga acacccagtc
      781 ccccatcttc gcccgcacgt ccgcggggat gatgccgcga ttctcgatgg tctgatatga
      841 gatgcagagc ggcgcgtgga ccgtgtacgg cgcgacgatc gcgccggtcg ggcagcggtc
      901 aaggcaaatc cggcagcggc cacagtcttt ggccagcggt tggtccgatt cgagctcgag
      961 gtcgatcagg atctcgccga gcatcacgaa cgagccatgg cccggcacga tgatgttcgt
     1021 gtgcttcccg taccagccca gcccggatcg agcagcgacc gcgcgatcga cgatccgggc
     1081 cgtgtcgacc agcaaccgca cttcaatgga cgagccgacc agagcctcga tccgggccgc
     1141 cagctcggcc atgcgcgtct tgagggtctt gtgatagtcg acgccccatg cgtaccgggc
     1201 gatccggccg cgcttgaccc cgtcatctgg cttctcgaca tcgttccgga agtagggaat
     1261 gccgacggag atgatcgact gcgcgcgctc atggagcatg cgagggttgg ccgagacgac
     1321 cgaccgctcc ggcgtgaacc agtccatgcc gcgcaaatga gcttgcgcga tgcggtcgtc
     1381 gaggagctcg gcgaggccgg gaaagggatc ggcgctcgag atcgaggcga ccgccagtcc
     1441 gcagtcgtgc gcgagtcgct tgatcgtcgc gctgaggtcg gttgcatgcg ggaagccggc
     1501 ggtcatgggc tcaacccggc ggcggccagc gcctcctcat acgtgggaag ggcggtctgg
     1561 gctccgaatt cagtcaccac gatgctcgcg accgcattgg cgaatcggag tgagcgctcc
     1621 aggtcccagc gaagcgccac ggcgaaggcc agcgctccgg cgaaggcatc acccgcaccg
     1681 gtcgtgtcgg ctaccgtcac ttcccgggcc ggggtggaaa gggatcgatc ccgtgtgacg
     1741 gccattgcac cagagtggcg acgggtcatg accgccaatc gaagattgtg gccgggcatg
     1801 gcctcggcca ccgattcaag ggcgtcggtg gcagacttga cgcccagtag ctcccggtac
     1861 tcacgatcgt taccaacgag gatgtcgtgg cgaagggcga cctcgagttt gtcgtccgcg
     1921 acaacatccg caaggtacgt cagtgttccg agaagccggg cggccggccg ggtatgagcg
     1981 ggcaggtcgc tcaagaactt gcgcaactcg tagtcgacgc agtcgactac ggtgacgtca
     2041 gcattgaaca gagcgtcgat atcgatccga tcgcgccgct tgattgaagg ttgctggtcc
     2101 cagaagatgg tacgttccga cgtttcccgg gagacgagca tctgcgagag gtccgaaacc
     2161 ttcagggagc gcatgcagcc gcctgtatcg accccagctt cgccgagagc ctccagaatg
     2221 cggtcccccc gctcgtcaca cccgaccaag gcatggagcg agacgtctgc gccaaggtgt
     2281 cgagcggcca tcgctgtgtt gccggtcgtg ccgcctgggc cgttaaacga tttgagaatg
     2341 aacgcgaagt ctccttcggc aggataccgg tcaatcagga gggtttcgtc ccagctgacg
     2401 acgccgatcg cagttatttt tggatggggc ttggtgtcag acatgagccg ctactaggac
     2461 gttatcggcg cggaaggagc gcgggaccgg acggtgaggg cgccgtgtgg gccagtcgct
     2521 ggcgcgccgg tccgcccggg aatgtgccga gcattgccat gaccagcatc gccagcacga
     2581 tcccaccgac gaggacccag tctttcgcga cctgattgcc ggtccagccg acgagtagcg
     2641 gcgccaggca ggccaccccg ctcacggcga gcgccagcca gcggtccctg tcgggccgaa
     2701 cgtcgaagag ctccgtaagg gccatccgct gcacagagac gccggcgccg atcaggagcc
     2761 cgacgatgta gatcagaagc gc
//
LOCUS       DASSGX010000435         3624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1055131, whole genome shotgun sequence.
ACCESSION   DASSGX010000435 DASSGX010000000
VERSION     DASSGX010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055131"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VFI12_07745"
     CDS             complement(<1..252)
                     /locus_tag="VFI12_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056341.1"
                     /translation="MRPAIGLLCCLLLTIAGFAASATAKAGSATPAATPFDCPLTEPG
                     GKQPPEIANLGGMQGRGNDALWVSLIMWSEHPGIVEVPND"
     gene            complement(341..670)
                     /locus_tag="VFI12_07750"
     CDS             complement(341..670)
                     /locus_tag="VFI12_07750"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HET7056342.1"
                     /translation="MAYDEGLVDRVREALSGEPGLTEKRMFGSYGFLLDGNMCAGVRN
                     DELIARVPLDEYPTILARPEVRPFPGPDRPMRGWITVGPDAIAEDDDLARWIKAGVAI
                     ARSLPPK"
     gene            complement(672..1868)
                     /locus_tag="VFI12_07755"
     CDS             complement(672..1868)
                     /locus_tag="VFI12_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HET7056343.1"
                     /translation="MAAVVDRVRGDIDEIMPGVIADRRQFHENPELGFQEFETAKIVA
                     ERLRSIGVEDVRTGIAVTGVTGLIHGGKGPGRVVLLRADMDALPIHEENKVEYCSKVP
                     GKMHACGHDAHTAMLLGIARVLHDRKDEFAGTVKVLFQPAEEGGGGAKVMIDEGVLSS
                     PDVDAVFGMHMDQGTPLGTISTRPGPAMAAADRFSATIKGKGGHGAHPDLTVDPIAVG
                     AQIVTALQTIVAREIDPIKPAVCTVGALIAGEAANVIPDTAILRGTLRSFDPDVREHL
                     AEAVERLVTGVASAMRAAVDYDYAQGYPATVNEPEMTRLVMDVAAEVVGPENVIEAPP
                     MMGAEDFSYFLEQKPGCFYFVGSANEDRGLTWGHHHPRFDIDEESMANGMDVMVRTVL
                     RYFETA"
     gene            complement(1963..2145)
                     /locus_tag="VFI12_07760"
     CDS             complement(1963..2145)
                     /locus_tag="VFI12_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056344.1"
                     /translation="MAIAITMPVATPNTMLIAFPSPAFPYTTLITAPRRHPAMMPIAR
                     VRRFIIRRHPIERMDS"
     gene            2385..>3624
                     /locus_tag="VFI12_07765"
     CDS             2385..>3624
                     /locus_tag="VFI12_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056345.1"
                     /translation="MPVPACRPNRRSAIRYIAILVACLVMLGALLPGTAAAFPRSLGP
                     TSWPVAEGGSFRYHVQPAMSGATAPSAEEFRTEFGPVFDTAERLATTILATSKVGPVD
                     VYAYSNLSLFQAMTKSRPIGLTPHAAVVVDTATSDVLVDLTTLLSLSTTQVEDAVRNA
                     VTQVAVQHAAGGAAPPAVAAGIGLYVELPTPEYLARLASTVQDAAQQNRLLAWFDLNR
                     PIASSDHELALGESYAMVAFLINRYDIPALRTLLAGLPKAASWQDAYRTAFAADAASI
                     EQQWRDDLPRWTTSGWRDNLMASFDLGPAKALLGQGQYVAAKALLDPSLNLYRQLNDP
                     TSLAETQALMAQADTGIQAEALMTEIETALREYDYVRAANLLDQADIQYKALPADQVP
                     QSLLMTYRQLATDGLNATAQL"
BASE COUNT          593 a         1203 c         1187 g          641 t
ORIGIN      
        1 gtcgttcggg acttcgacga tgcccgggtg ctcgctccac atgatgaggc tgacccagag
       61 cgcgtcgttc cccctgcctt gcatccctcc gaggttcgct atctcgggtg gttgcttgcc
      121 accgggttca gtgaggggac aatcaaatgg ggtggccgcg ggggtcgcac tgcccgcctt
      181 cgcggtcgcg ctggcggcga atccggctat cgtcaggagc aggcagcaga gcagtccaat
      241 cgccggtcgc atcgcggtta cctccgatta tggcgcgtct gcccgtagta caaccgccgc
      301 cggcaatcgg ttccaccgca caccgtccga tcggttcagg ctacttcgga gggagggagc
      361 gggcgatggc gacgccggct ttgatccagc gcgcgaggtc atcgtcttcc gcgatggcgt
      421 ccggaccgac ggtgatccag ccgcgcatgg ggcggtccgg ccccgggaag gggcggacct
      481 ccggccgggc caggatagtc ggatactcgt cgagtggcac ccgggcgatc agctcgtcat
      541 tgcggacgcc ggcgcacatg tttccgtcga gcaggaagcc gtagctgccg aacatgcgtt
      601 tttcggtcag gcccggctcg ccggacaggg cctcgcggac gcgatccacg aggccctcgt
      661 cataggccat ctcaggcggt ttcgaagtag cggagcacgg tccggaccat cacgtccatg
      721 ccgttcgcca tcgattcctc gtcgatatcg aagcgcggat ggtggtggcc ccaggtcaga
      781 ccgcgatctt cattggcgct gcccacgaag tagaagcaac cgggcttctg ctcgaggaag
      841 tagctgaagt cttccgcgcc catcatcggc ggcgcctcga tcacattctc cggcccaacg
      901 acctcggcgg cgacgtccat caccagccga gtcatctccg gctcgttgac ggtcgcgggg
      961 tagccttggg cgtagtcgta gtcaaccgcg gcgcgcatcg cgctcgcgac gcccgtgacc
     1021 agccgctcga ccgcctcggc aaggtgctcg cggacgtcag gatcgaagga acggagtgtg
     1081 ccgcggagga tggccgtatc cgggatgacg ttggcggcct caccggcgat cagggcgccg
     1141 acggtgcaga cggcaggctt gatcgggtcg atctcgcgag cgacgatcgt ctggagcgcg
     1201 gtcacgatct gggcgccgac cgctatcggg tcgacggtca ggtccgggtg cgcgccgtgg
     1261 ccgcccttgc ccttgatcgt ggcggagaag cggtcggccg cggccatcgc gggaccaggc
     1321 cgggtcgaga tcgtgccgag cggagtgccc tggtccatgt gcatgccgaa gacggcatcg
     1381 acatcgggac tggacaggac gccctcgtcg atcatgactt tcgcgccacc gccgccctct
     1441 tccgccggct ggaaaagcac cttgaccgtg ccggcgaact cgtccttgcg gtcgtgcaaa
     1501 acgcgggcaa tgccgagcag catcgcggtg tgcgcgtcgt ggccgcaggc gtgcatcttg
     1561 cccgggacct tcgagcagta ctcgaccttg ttctcctcgt ggatcgggag cgcgtccata
     1621 tcggcccgga gcaggacgac tctgcccggc cccttgccgc cgtgaatcag gccggttacg
     1681 ccggtgaccg cgatgccggt ccggacatcc tcgacgccaa tcgagcgcag ccgctcggct
     1741 acgatcttcg ccgtctcgaa ctcctggaac ccgagctccg gattctcgtg aaactgtcgc
     1801 ctgtccgcga tcacgccggg catgatctcg tcgatatcgc cgcgcacccg atcaactact
     1861 gctgccacgc ctggactcct ccttaccgat aacgaacgag cgtcgcgctc gcactggatg
     1921 gcggtgattg tacggggtcc tgctcgccta tgatgaggct ccctaagaat ccattcgctc
     1981 gatggggtga cgcctgatga tgaaccggcg aacacgcgca atcggcatca tggccgggtg
     2041 tctcctcggc gcggtaatca gcgtggtgta cgggaaggcc ggactcggaa acgcaatcag
     2101 catagtgttc ggggttgcga ccggcatggt gatcgcgatg gccattgcgt ccgccaggag
     2161 gtgagcccaa cgcgtggctc cccgcgagac ggtgtgctag gctgaccgtt ccattccggg
     2221 acggggaaga gggatggttt gcccgtgcat tgcgtgccgg atgcgctgct tcggcgtaac
     2281 cggttatagt gcgtcgaatg gttgacacgc gttcataatg gagttgcgcc cggcgcgggc
     2341 cagcctcgac gctccgctga gatcgaacca ggagaagcgg tacgatgccc gtacccgctt
     2401 gccgtccgaa tcgcaggtct gcgattcgat atatagcgat cctcgtcgcc tgccttgtca
     2461 tgctcggcgc gttgctgccc ggcacggccg cggcattccc gcgatcactc ggtccgacaa
     2521 gctggccagt cgcggagggt ggctcgttcc gctaccacgt ccaaccggcg atgtccggcg
     2581 cgacagcgcc gagcgccgag gaattccgga ccgagtttgg gccggtgttc gatacggcgg
     2641 agcgactggc gacgactatc ctggccacgt caaaggtcgg cccggtcgat gtctacgcct
     2701 acagcaacct ctccctcttc caggcaatga ccaaatcgcg tccgatcggg ttgacgcctc
     2761 acgccgcggt tgttgtcgac acggcgacgt ccgacgtgct ggtcgacctg acgacactgc
     2821 tctccctttc gacgacacag gtcgaggacg ccgttcgcaa cgcggtgacg caggtcgcgg
     2881 tccagcacgc ggcgggtgga gcggcgccgc cagcagtcgc cgcggggatc gggctttatg
     2941 tcgagcttcc gacaccggaa tacctcgccc ggctggcatc gaccgtccag gacgcggctc
     3001 agcagaaccg gctcctcgcc tggttcgacc tcaatcgccc gattgcgtct tccgaccatg
     3061 agctcgcgct cggcgagtcc tacgcgatgg tcgccttcct gattaatcgt tacgacatcc
     3121 cggcgttgcg gaccctcctg gccggcctgc ccaaagcggc atcctggcag gacgcgtacc
     3181 ggacggcatt cgccgccgac gccgcgtcga tcgagcagca gtggcgcgac gatttaccac
     3241 gctggacgac ctccggctgg cgggacaacc tgatggcctc gttcgatctg ggacccgcca
     3301 aggcgctcct cgggcagggg caatatgtcg cagccaaggc gctgctcgac ccgtcgctca
     3361 acctctaccg ccagctcaac gatcccacgt cgctggccga gacgcaggcc ctgatggcgc
     3421 aggcggacac cggcatccag gccgaggcgc tgatgaccga gatcgaaaca gcgctccgcg
     3481 aatacgacta tgtccgggcg gcgaatctgc tcgatcaggc ggatatccag tacaaggcgc
     3541 tgccggcgga tcaggttccg caatcgctct tgatgaccta tcgccaactg gccaccgacg
     3601 gtctgaacgc gaccgcgcaa ctcg
//
LOCUS       DASSGX010000436         3342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_292954, whole genome shotgun sequence.
ACCESSION   DASSGX010000436 DASSGX010000000
VERSION     DASSGX010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292954"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VFI12_07770"
     CDS             <1..216
                     /locus_tag="VFI12_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056346.1"
                     /translation="IGMLSPLPGGAGIRETLMGGIARIHGAAVSPTIFAAIAYRIALF
                     ASIPVLYLLFRIWLSLRKQPSIELEDQ"
     gene            213..842
                     /locus_tag="VFI12_07775"
     CDS             213..842
                     /locus_tag="VFI12_07775"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7056347.1"
                     /translation="MSTTVHLSAEDTSNYIKHTHPNPIQRKLIDRFHKTVLQKIEDLH
                     PASFLDAGCGEGFVASLILERVPDAQLTGFDFNPASVDMAKAMNPTANFVVASIFEIP
                     FGPQSFEVTGCFEVLEHQTDPAGALRELARVTARSLILSVPHEPYFCLANVARGKNLD
                     IRPRGSDPDHKQFWTRAKFGEFVTATCPEFQIEWLGGSLPWTICIATRK"
     gene            926..2470
                     /locus_tag="VFI12_07780"
     CDS             926..2470
                     /locus_tag="VFI12_07780"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET7056348.1"
                     /translation="MSHSTRRCPNCSQFNYGKAEFCQACGEDISKVEAVFIPWAQQHL
                     LPPIAAAVREEGRRFARVDPDAAGSGLVWTGLLVIAAALLIDIPSHALALLVSGGTVV
                     VIVGLWQLRIDYGALSRLGGWLVGVSIFALAIVAWRVVEPDTPNPVHLIQSKPTAVPQ
                     VAAALVGDGEVPMQRGGPAHLGVNPGPAPTGSLYRSWRFNTGGELYSSPAVAEDMLVI
                     GSKSGFLYGLNAVSGEQVWSRDLGQYIVRSTPAIQDGVVFVNNGYQTLALSLKDGSTI
                     WTTDISFAGNTSPTVDNTTVYVASQSGAVSALDAQTGKQRWRAQLQGLIFGSPTVDAG
                     SVIVANDGGMVYGMAIANGQVNWRFTADGGIFAPLISDEGVVYFTSNAGSTYAINGDS
                     GEQIWRYDAGGASGGSVTLDTVIVGADNGGVSAINRETGKIDWLVPTGSQITVGPTIV
                     GRLVIVASGQTLYGLDIKTGEQQFTYATGYTIQVAPTVVNGAIYVGGRDGYLDAIVGD
                     APAKSH"
     gene            complement(2467..>3342)
                     /locus_tag="VFI12_07785"
     CDS             complement(2467..>3342)
                     /locus_tag="VFI12_07785"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HET7056349.1"
                     /translation="NEAILAQPPGTIQGRIKLTEQGEVISARYSIPEIAIREFELVVG
                     AVLVASFGGLQSLPKDELGPYQDVMGRLADWSREAYRELVYGDPGFVDFFQQATPIRE
                     ISELKLGSRPARRTASTDIEDLRAIPWVFSWTQARMVLPGWYGLGAALEHGCAEFGIE
                     PLRRMYRLWPFFATTISNAEMALSKADMSIAERYVAMVEPAELRDRIWGKIRSEFDRT
                     QAMILEITGETHLLEHDPVLRRSIERRNPYVDPLSFLQLELIQRLRTTRRHDELIRPV
                     LLTINGIAGALKNTG"
BASE COUNT          578 a         1129 c         1030 g          605 t
ORIGIN      
        1 atcggcatgc tcagcccgct acccggcgga gcgggcatcc gggagacgtt gatgggcggc
       61 atcgcccgga tccacggagc agccgtctcg ccgactatct tcgccgcgat cgcctaccgg
      121 atcgccctgt ttgcctcgat cccggtcctg taccttttat tccgcatttg gttgtcgctt
      181 cggaagcagc cgagcatcga gttggaggat caatgagcac cacggttcac ctgagcgccg
      241 aggatacgag caattacatc aagcacaccc atcccaaccc catccaacgc aagctcattg
      301 atcgctttca caagacggtt ctccagaaga ttgaagatct ccatccggcc tcattcctgg
      361 atgccggttg cggcgaaggc ttcgtcgcca gcctgatcct cgaacgggtg ccggatgcgc
      421 aattgaccgg cttcgatttc aacccggcct cggtcgacat ggccaaggcg atgaacccga
      481 cggctaattt cgtcgtcgcc agcatctttg agattccgtt cggtccgcag agcttcgagg
      541 tcaccggctg cttcgaggtg ctcgagcacc agaccgatcc ggccggggcg ctccgggagc
      601 tggcccgcgt tacggcgcga tcgctgatcc tcagcgtgcc gcacgagccc tacttctgcc
      661 tggccaatgt ggcccgcggc aagaacctcg atatccggcc gcgcggcagc gatcccgacc
      721 acaagcagtt ctggacccgg gcgaaattcg gcgaattcgt gaccgcgacc tgtcccgagt
      781 tccagatcga gtggctcggc ggatccctcc cctggacgat ctgtatcgcc acccgcaaat
      841 aggaactgct gtctgcatgt gatagcctga gtactccaca ccgactcgcc ccggacatga
      901 gcggcgagcg gaccggagta ccggcgtgag ccactcaacg cgtcgctgtc cgaactgcag
      961 ccagttcaat tacggcaagg ctgagttctg ccaggcgtgc ggcgaggaca tctcgaaagt
     1021 cgaggctgtc ttcatcccct gggcccaaca gcacctgttg cccccgatcg cggccgcggt
     1081 gcgtgaggag gggcgtcgct tcgcgcgggt tgacccggat gcggccggca gcggcctcgt
     1141 ctggaccggg ctgctggtta tcgccgccgc cctcctgatc gatatcccct cgcacgccct
     1201 cgctctactg gtctccgggg gcacagtcgt cgtcatcgtt ggcctatggc aattgcgaat
     1261 cgactacggc gcgctcagcc ggctcggcgg ctggttggtc ggcgtgtcga tcttcgcgct
     1321 ggcgatcgtc gcctggcgcg tcgttgagcc cgatacgccg aacccggtgc atctgatcca
     1381 gtcaaaaccg accgccgtgc cccaggtcgc cgcggctctt gtcggcgacg gcgaagtgcc
     1441 gatgcaacgg ggcggcccgg cgcacctggg cgtcaacccg gggccagcac cgaccggctc
     1501 actttaccgc tcatggcgct ttaacaccgg gggcgagctc tactcctcgc cggcggtcgc
     1561 agaagacatg ctggtcatcg ggagcaagag cggcttcctg tacggtctga acgccgtttc
     1621 cggcgagcag gtctggtcgc gcgacctggg ccagtacatt gtccgttcaa cgccggcaat
     1681 ccaggacggc gtggtcttcg tcaacaacgg ctaccagacg ctcgctctct cgctcaagga
     1741 cggttcgacc atctggacaa ccgacatttc ctttgccggc aacacgtcac cgaccgtcga
     1801 taacacgacg gtctacgtgg ccagccagag cggcgctgtc tccgcgctgg atgcccaaac
     1861 gggtaagcag cgctggcggg cgcaactcca ggggctgatc ttcgggtcgc cgacggtcga
     1921 tgcgggcagc gtgatcgtcg cgaatgacgg gggcatggtg tacggcatgg ccatcgccaa
     1981 cgggcaggtc aattggcgtt tcacggccga tggcggcatt ttcgccccac tgatcagcga
     2041 tgagggcgtc gtctacttca ccagcaacgc cggctcgacc tatgcgatca acggcgactc
     2101 cggcgagcag atctggcgat acgacgccgg cggggcgagc ggcggctcgg ttacgctcga
     2161 caccgtcatc gtcggcgcgg acaacggcgg tgtctcggcc atcaatcgcg agaccggcaa
     2221 gatcgattgg ctcgtcccca ccggtagcca gattaccgtc gggcccacga ttgtcggccg
     2281 gctcgtgatc gtcgccagcg gccagacact ctacgggctc gatatcaaaa ccggcgagca
     2341 acagtttacc tacgccaccg ggtacaccat ccaggtcgcc ccgaccgtcg tcaacggcgc
     2401 gatctacgtc gggggccggg acggctacct cgatgcgatc gtcggtgatg ccccggccaa
     2461 atcccactaa ccagtgttct tgagcgcgcc ggcgatgcca ttgatcgtga gcaggaccgg
     2521 gcggatcagc tcgtcatgcc ggcgcgtcgt ccgcaaccgc tggatcagct cgagctgcag
     2581 gaatgagagc ggatcgacgt atgggttgcg ccgttcgatc gaccgccgca aaacgggatc
     2641 gtgttccagc agatgcgtct cgccggtgat ttcgaggatc atcgcctgag tccggtcaaa
     2701 ttccgaccgg atcttgcccc agatgcggtc gcgcagctcg gccggctcga ccattgccac
     2761 gtatcgttcg gcaatcgaca tgtcggcctt gctgagcgcc atctcggcgt tgctgatcgt
     2821 cgtggcgaag aacggccaca gccggtacat gcgccgcagc ggctcaatcc cgaactcggc
     2881 gcagccgtgc tcgagcgccg cacccagtcc ataccagccg ggcagcacca tccgcgcctg
     2941 cgtccacgag aagacccagg ggattgcccg gagatcttcg atgtcagtcg acgctgttcg
     3001 ccgcgccggg cgcgacccga gcttcaactc cgagatctcc cggatcggcg tggcttgctg
     3061 gaagaagtcg acgaatcccg ggtctccgta gaccaactcc cggtaggctt cccgggacca
     3121 gtctgccaac cgtcccatca catcctggta gggtccaagc tcgtccttcg gcaggctctg
     3181 caggcccccg aaactggcca ccagcactgc cccgacgacc agctcgaact ccctgatcgc
     3241 gatctcgggg atggagtagc gcgcggagat gacctcgccc tgctccgtca gcttgatccg
     3301 gccctggatc gtgcccggcg gctgggcgag gatcgcctcg tt
//
LOCUS       DASSGX010000437         3879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1165841, whole genome shotgun sequence.
ACCESSION   DASSGX010000437 DASSGX010000000
VERSION     DASSGX010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165841"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="VFI12_07790"
     CDS             <1..909
                     /locus_tag="VFI12_07790"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET7056350.1"
                     /translation="AARILRKGKSGAYGILLTESLSYAVTDPAAVILLQGIAEVFDER
                     DAGLLVLPSRGDRSTGLEAVRNAVVDGFLIYSIAEDDPRLTAVLARNVPTVVIEEPNL
                     PNIARVSLEDREGARTVAEHVVGLGHRPLAAITFPLAEDDWSGFVGAERLARSTIRVS
                     RDRLAGFADAAETAGIDWSGAPVYECVLNSVEEGRLAAGKLLDGQVRPTAILTTADQL
                     AFGAILAARERGLRVPEDLSVAGFDDIPEAARSDPPLTTVRQPLREKGTIAARLLLDG
                     WEGPPPTKILPTELVVRDSTGPAPAQ"
     gene            949..1173
                     /locus_tag="VFI12_07795"
     CDS             949..1173
                     /locus_tag="VFI12_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056351.1"
                     /translation="MTGYQIIHDLQRVTALEARSAQATAPTVNESERPVRTPRLATLR
                     LQATVYLRRLADALEPAPLDTVPSRNGSGC"
     gene            1340..1765
                     /locus_tag="VFI12_07800"
     CDS             1340..1765
                     /locus_tag="VFI12_07800"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7056352.1"
                     /translation="MVGREQGAQRIFIWCFSEQAGELLGPHWHDGEEFFRVLSGRLRF
                     KVGDEISEVGAGEVVVIPPKVLHSHTVLEDCELEVIGEISSGVYVQVTGTDGEFSEQE
                     LFVRDVPWSRLPARASYYITRDEQLERFRLASDGDATFP"
     gene            complement(1762..2742)
                     /gene="add"
                     /locus_tag="VFI12_07805"
     CDS             complement(1762..2742)
                     /gene="add"
                     /locus_tag="VFI12_07805"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HET7056353.1"
                     /translation="MRDITALPKAHLHLHFEATARPETIHELADRAGVPYRVPNVFAS
                     FADFDAAYTAMVGLILEPEDVARIAREIVEDEAAQGVGYTEPMINPATYAAQFGISVG
                     EAFDLMRDAFFGAGEELGVEVGLMIAGIWGFADEVNEAAARFAAEQSDRGVVAFGLAG
                     VEPQGGYARALRACDIARDAGLAVIPHAGEFGGPANVREAMDVLRGDRIAHGVRAIED
                     PDVVALLADRQMVCDVAPSSNVILGVFPEMGLVPIAHFLEAGVPFTLNSDDQLFFVNR
                     VQDEYVVVRDTFGLSDVEVAAIARTSVTASRASARTKQDMLAGIDAWLAS"
     gene            2962..3399
                     /locus_tag="VFI12_07810"
     CDS             2962..3399
                     /locus_tag="VFI12_07810"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HET7056354.1"
                     /translation="MSTDENKAVIRKLFAAIDAHNLDLVEQELIDPGYHLHFDSMPEM
                     DKAGAVASFRAFVTGFPDISHTLEEMIAEGDRVGCRLTVRGTNTADFMGMPATNRAIE
                     ISSINVFRLKDSLIVNQQINSDGVGMMRQLGLIPDSGQPQERV"
     gene            3399..3839
                     /locus_tag="VFI12_07815"
     CDS             3399..3839
                     /locus_tag="VFI12_07815"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HET7056355.1"
                     /translation="MDTTSNKAIDRRYVDEVLNGRNLDAIDQICAESYVSHVPGMPDM
                     DRAGDKQLVGSMFAGFPDLEFEVGDQIAEGDRVLHTLTGRGTHQAEFMGIPPTGRRVE
                     VTGMNINRIAGGKIAESWGVIDILGLLQQLGVVPTPGASVGVEG"
BASE COUNT          712 a         1315 c         1221 g          631 t
ORIGIN      
        1 gcagcgcgca tcctccgcaa gggcaaaagc ggcgcctacg gcatcctcct gacggagagt
       61 ctctcctatg ccgtcaccga tccggccgcg gtcatcctcc tccaggggat cgccgaggtc
      121 ttcgacgagc gcgatgccgg cctgctcgtc ctcccctccc ggggcgatcg ctcgaccggc
      181 ctcgaagcgg tcaggaacgc agtcgtcgac ggcttcctga tctactcgat cgcggaagat
      241 gacccgcgcc tgaccgccgt gctggcccgc aacgttccaa ccgtcgtgat cgaggagccg
      301 aacctgccca acatcgcccg ggtctcgctc gaagaccggg aaggcgcgcg aacggtcgcg
      361 gagcacgtgg tcgggctcgg acaccggcca ctcgccgcca tcacgttccc cctcgccgag
      421 gacgactgga gcggcttcgt cggcgctgaa cggctcgcgc gatcgacgat tcgggtctcg
      481 cgagaccggt tggccgggtt cgccgacgcg gccgagaccg ccggaatcga ttggtcgggc
      541 gcgccagttt atgagtgcgt gctcaactcg gtcgaggagg gacgactcgc ggccgggaaa
      601 ctgctcgatg ggcaagtccg gccaaccgcg atcctgacca cggccgacca gctcgcgttc
      661 ggcgcaatcc tggccgcgcg ggagcgggga ttgcgcgtcc cggaggacct ctcggtcgcc
      721 gggttcgacg acatcccgga ggcggcccgc tccgatccgc cgctgaccac cgtccggcag
      781 ccgctgcgcg agaagggaac catcgcggcc aggctgctgc tcgatggctg ggagggcccg
      841 ccgcccacga agatcctgcc gaccgagctg gtcgtgcggg actcgactgg tcccgccccg
      901 gcccaataac ccgacacgat tcgacctatc aaggtagagg aaatgactat gaccggctat
      961 cagatcatcc acgacctcca gcgcgtcacc gcattggaag cacgctccgc ccaagccacc
     1021 gccccgaccg tcaacgagtc ggagcgaccg gtccggacac cacgccttgc cacgctccga
     1081 ttgcaagcga ccgtctacct gcgccggctg gcggatgccc tcgagccggc cccgctggac
     1141 accgttccga gccgtaacgg ctccggatgc tgagggcaac accattccgt tacgatcacc
     1201 cggccgcgtt tcatgcagtc ccggccgggt ttgttttggc cggttccggt atcattgccg
     1261 ctcaacccag caatctctct ttatcgcgaa gggccagcag atgaatgtcg tcaacctccg
     1321 tggtttggat gcacacccca tggttggccg cgagcagggc gcccagcgca tcttcatctg
     1381 gtgcttctcg gagcaggccg gcgaactcct cggtccacac tggcatgacg gcgaggaatt
     1441 cttccgcgtt ctctccggcc ggttgcggtt caaggtcggc gacgagatta gcgaggtcgg
     1501 cgcgggcgaa gtcgtggtca tcccacccaa ggttcttcat agccacacgg tcctcgagga
     1561 ttgcgagctc gaagtgatcg gggagatcag ctccggcgtc tacgtccagg tcaccgggac
     1621 cgacggcgaa ttctccgagc aggagctctt cgtgcgggat gtgccctgga gccggctccc
     1681 ggcccgcgcc agctactaca tcacccgcga cgagcaactc gaacgcttcc gcctcgcctc
     1741 agacggcgac gccaccttcc cctaactcgc cagccaggcg tcaatgcctg cgagcatatc
     1801 ctgcttcgtc cgagcgctgg ctcgggatgc ggtgacgcta gtccgggcga tagcagcaac
     1861 ctcgacatcg ctcaggccga aggtgtcgcg tacgacaacg tactcgtcct gcactcggtt
     1921 gacgaaaaag agctggtcgt ccgagttgag cgtgaagggc acaccggcct cgaggaagtg
     1981 cgcgatcggc accaacccca tttccgggaa gacgccaagg atcacgttcg agctcggcgc
     2041 cacgtcgcag accatctgcc ggtcggccag cagcgcaacg acgtcagggt cctcgatcgc
     2101 tcgtaccccg tgcgcgatcc ggtcgccgcg caggacgtcc atcgcctccc gcacgttggc
     2161 gggcccgccg aattcgccgg cgtgcggaat cacggccagg ccggcgtcgc gggcgatgtc
     2221 gcaggcccgc aatgcgcgag catacccacc ctgcggctcg acaccggcca acccgaacgc
     2281 gaccacgccg cgatccgact gctccgccgc aaatcgggcc gccgcctcgt ttacctcatc
     2341 cgcgaagccc cagatcccgg cgatcatcag tccgacctcg acgccgagct cctcgccggc
     2401 cccgaagaag gcatcccgca tcaaatcgaa cgcctccccg acgctgattc cgaactgcgc
     2461 ggcataggtt gccgggttga tcatgggttc ggtatagccg accccctgcg ccgcctcatc
     2521 ctcaacgatc tcgcgggcga tccgcgcgac atcctctggc tcgaggatca agccgaccat
     2581 ggcggtatac gcagcatcga aatcggcgaa tgacgcaaag acgttcggga cacggtaggg
     2641 aacgccggcc cgatcggcta actcgtggat cgtctccggc cgggcagtgg cctcgaaatg
     2701 caggtgcaga tgggccttgg gcaacgcggt gatatcgcgc acgggacctc cgggatcacg
     2761 agatgggcaa aagcagattg accgggaatg gtacgggacc gaacccaaat cagcacgcag
     2821 tgaaccggcg ccaaggacaa tccgtatcag ttattgacag caatcagttc tctcgtaaag
     2881 tggattcgtt tcatacacag gcgcggcaat ccgcgcggtt gttgtcatga ccggtcgact
     2941 ggacccagga gatgcgctgc tgtgagtacg gacgagaaca aggccgtaat ccggaagttg
     3001 ttcgcggcga tagacgcgca caacctcgac ctggtcgagc aggagctgat cgatcccggc
     3061 tatcacctcc acttcgactc gatgccggag atggacaagg cgggtgccgt tgcctccttc
     3121 cgggcgttcg tgaccggctt ccccgacatc tcgcacaccc tcgaggagat gatcgccgag
     3181 ggcgaccgcg tcggctgccg cctgaccgtg cgcggcacga acaccgccga cttcatgggc
     3241 atgcccgcca ccaaccgggc gatcgagatc agctcaatta acgtgttccg gctcaaggac
     3301 agcctgatcg tcaatcaaca gatcaactcg gacggcgtcg gaatgatgcg gcagctcggc
     3361 ctgatccccg attcgggcca gccacaggag cgcgtgtgat ggatacgacc tcaaacaagg
     3421 cgatcgatcg ccgctacgtc gacgaggtcc tgaatgggcg caacctggac gcgatagacc
     3481 agatctgcgc cgagagctac gtcagccacg tcccgggcat gccggacatg gaccgcgccg
     3541 gcgacaaaca gctcgtcgga tcgatgttcg ccggtttccc ggacctggaa ttcgaggtcg
     3601 gcgaccagat cgccgagggt gaccgcgttc tgcacactct gaccggccgg ggcacgcacc
     3661 aggctgagtt catgggcatt ccgccgaccg gccggcgcgt cgaagttacc gggatgaata
     3721 tcaaccggat cgcgggcggc aagatcgccg aatcgtgggg cgtgatcgac atcctcggcc
     3781 tcctccaaca actcggcgtc gtgcccacgc ccggcgcgag cgtaggggtt gagggttgag
     3841 ggttgagggt tgagggttga gggttgaggg ttgagggtt
//
LOCUS       DASSGX010000438         4718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_949415, whole genome shotgun sequence.
ACCESSION   DASSGX010000438 DASSGX010000000
VERSION     DASSGX010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4718
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949415"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VFI12_07820"
     CDS             complement(<1..485)
                     /locus_tag="VFI12_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056356.1"
                     /translation="MAESRLQFDPSADLERALREIAPVVSFPPTPDLADTIRARIAAE
                     QASSRPARSWSALLRPRRLAVAFLVLLLVVAAALTLSPGLRTSVANRLGVHGIEIIFV
                     EETPTPAATPVGTTLLLGENVTLAEAQARAGYPIQIPAELGPPDEVYLRQRSTGPMVT
                     LL"
     gene            complement(478..1092)
                     /locus_tag="VFI12_07825"
     CDS             complement(478..1092)
                     /locus_tag="VFI12_07825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7056357.1"
                     /translation="MYNERTIDRGRPPLDESIAIERARAGDGVAYETVVRQYQDIAFR
                     TAWMITGDAAEAEDAAQSAFVKAYFALGRFRSGAPFRPWLLQIVANEARNRRTATRRH
                     PTIDLATIADRPDPATGDAPETAALAGDQRERLVAALNHLREEDRIVIVCRYFLDLTE
                     AEMASALDCPRGTVKSRLSRALGRLREHLTATAGAGDQRRESDG"
     gene            1469..>4718
                     /locus_tag="VFI12_07830"
     CDS             1469..>4718
                     /locus_tag="VFI12_07830"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HET7056358.1"
                     /translation="MPARSAFASSNCVARWLIALILLLGVLAPLAPTGAVAQDAPAGE
                     TAPPDGTPEATSPAPTEAPAEPTSIPAPTDTATPAPPAPTATPSPSPTPTQTAAPTAT
                     RQATAPIVVAATYSVTILPKDPNGVALLGVCFELHHDGGNKTIGARMRTICDWNFEGK
                     LVTALAPGDYVLLETTHPDTVGRALPKFFTVTGTRSLTVNHQWLIDIVVTAKDDVTGG
                     AARSGCYSARLVDVSNWVSACDADDGTADGITAIHGIDRGTWQIETFPVAPEGYLPMV
                     PKQVTVTSLSGPFAVAVLHTNGGTLNVAVTNQAGDPLLDACFQLAEDAGSGTPGGQVD
                     YRCDNADGTSDGTVTLTAIPAGNYVLRQQGAAPGYLPAADQAVSITSGQTTNTTIVNR
                     PGGVVVIHARDDLGNPINDICVATYQDAGGGALGTFMGLPCVQGDSATDGESRIIGFP
                     TGNYVLIQLTSQSGYVRAAPKRISVTEGTTTTITIVNPLGGALTVHHQDNTGTAVVDG
                     CFRVVKDAGGGTRGEAVGDSVCDAYDSARDGDTHITGLPSGSYLLVDLSFSPTRSNSA
                     DIPFTIATRQTKELTVTSVNHGTLRLARTDAGGATVLGGCVDVFWAAGTPNAGQQVIF
                     NACDGLRDVADGTLELRLPNGRFEVRDGNLPLGYVQGDPITVEIIDSGLTEATIVTKR
                     MPDLTVRAVDTWGDPVKDVCVLLFPDETSGNTDMEQAVCDSADGATDGRILFRTLVGP
                     GVWLVRSTPDSSYPFAATSITLAADEVRTLDVIVTMPAPKILVGPLLMDITDTSATVY
                     FTTDQKTVSTVAFGTGDPFDQSAPATAETPRKTHRIQITGLQPNTIYDWSLTTGNGYG
                     SANADGWRFRTGASNAQIVIKKVDSAGSTLKGACFELYTDAGNGALGTYIMFECDAFE
                     ADGSNGLITFGGLDPGSYVVTEMSAPSKFKLAAPVRTTLTAGQIKQLTVTDYRGGTVV
                     KIRNEAYGHELLPGSCFYVYKRGSGESLGAYVTWSCDEFDGTDASTTVSGLPVGNYVL
                     VQSYVPDGFVEAEYMSFSITSSTGSSLSLTVVNHQTNGEQVVAVRTVNAAGTL"
BASE COUNT          797 a         1626 c         1567 g          728 t
ORIGIN      
        1 aggagcgtga ccatcggccc ggtcgagcgc tggcgcaggt aaacctcgtc tggcgggccg
       61 agctcggccg ggatctggat cgggtagccg gcgcgggcct gcgcctcggc cagcgtcacg
      121 ttctcaccga gcagcagcgt ggtcccgacc ggggtagcgg cgggggtcgg cgtctcctcg
      181 acgaagatga tctcgatgcc gtggacgccg agccggttcg cgacggaggt ccgcagcccc
      241 ggcgagaggg tgagcgccgc agcaacgacg agcaacagga cgaggaaggc gaccgccagc
      301 cgtctgggac ggagcagggc agaccacgac ctggccgggc gggatgacgc ttgctcggcc
      361 gcaatgcgcg cccggattgt atcggccaga tcgggcgtcg gcgggaagga aaccaccggc
      421 gcgatctccc gtagcgcgcg ctcgaggtcg gcggagggat cgaactggag gcgcgactca
      481 gccatcggac tccctccgct ggtcaccggc gccggccgtg gcggtcaagt gctcccgcag
      541 ccggccgagc gcccgcgaga ggcgggattt gaccgtgccg cgcggacaat cgagcgcact
      601 ggccatctcc gcctcggtca ggtccaggaa gtagcggcac acgatgacga tgcgatcctc
      661 ctcccgcagg tgattgagcg ccgccaccag ccgctcgcgc tggtcgccgg cgagcgcggc
      721 cgtttccggc gcgtcaccgg tggcgggatc cgggcgatcg gcgatcgtgg caaggtcgat
      781 agtcggatgg cggcgggtgg cggtgcgccg gttgcgggct tcgttagcga cgatctggag
      841 cagccagggc cggaacgggg cgccggaccg gaaccggccc agcgcgaaat aggccttgac
      901 gaaggcggat tgggcggcat cctcggcctc ggccgcatcg cccgtgatca tccaggcggt
      961 gcgaaaggca atgtcctggt actgccgcac aaccgtttcg taggcgacgc catctccggc
     1021 gcgggcgcgc tcgatcgcga tcgactcgtc cagtggaggc ctcccacggt cgatagttct
     1081 ctcgttatac aaccggcagg gagtgatggt tcccgcgtac ccgggtggag ggaaatggag
     1141 gcgcagatgg catctgcccg ctccgcccac ggccgcaccc gaccgtagcc ccggcccccg
     1201 tggccggttc gatgcgcagg ccttcggttc tccgcccgcc gatgcggcac gactggtcag
     1261 ggcttttcgc gcatcctata catttacatt cgccatagtt tgccgcataa tccattcaga
     1321 gccgcaacat cgtctcctaa ggatcagggg tagatcgcgc aggcgatatg actgtcgctt
     1381 ggcgcgggac gcccgtggtc agcgcccccg cgcaggatgc cggaccccgc gtcccggttt
     1441 ggcgacccat ttttagggga gaattgtcgt gccagctcga tccgcctttg cttcatccaa
     1501 ttgcgttgcg cgatggctca ttgccctgat cctgctgctc ggcgtcctgg cgccgcttgc
     1561 gccaaccggt gcggtcgcgc aggacgcccc ggccggggag acggcgccgc cggacgggac
     1621 gcccgaggcc acgagcccgg ccccgaccga agctcccgcc gagccgacat cgatcccggc
     1681 gccgactgac acggccacgc ccgctccgcc ggctccgacc gccacgccga gtccctcgcc
     1741 aaccccgacg cagacggccg cgccgaccgc gacccggcag gcaaccgcgc cgatcgtcgt
     1801 cgccgcgacc tactccgtca ccatccttcc gaaggatccg aacggtgtcg cgctgctcgg
     1861 tgtctgcttc gagcttcatc acgacggtgg caacaagacc atcggcgccc ggatgcgaac
     1921 catctgcgac tggaacttcg aagggaaact ggttaccgcg ctggcgcccg gggattatgt
     1981 ccttctcgag accacccatc cggacacggt cggcagggcc ttgccgaagt tcttcaccgt
     2041 gaccggcacc cgcagcctca cggtcaatca ccagtggctg atcgacatcg tcgtcaccgc
     2101 gaaggacgac gtcaccggcg gggccgcgcg gtccggttgc tattcggcac ggctcgttga
     2161 cgtctcgaat tgggtctccg cgtgcgacgc cgacgacggc actgccgacg gcatcaccgc
     2221 gatccacggc atcgaccggg gcacgtggca gatcgagacg ttccctgtcg cgccggaggg
     2281 ctacctgccg atggtgccga agcaagtcac ggtgacgtcc ctctccgggc cgttcgcggt
     2341 cgctgtcctg catacgaacg gcggcacgct caacgtcgcg gtgaccaacc aggcaggcga
     2401 tccgctcctc gacgcctgct tccagctcgc cgaggatgcg ggcagcggga cgcccggcgg
     2461 gcaggtcgac tatcgctgcg acaacgccga tggcacgagt gacgggaccg tgacgctcac
     2521 ggcgatcccg gccggaaact acgtgctgcg ccaacaaggc gctgcccccg gctacctgcc
     2581 cgcggccgat caggccgtgt cgattaccag cggccaaacc acgaacacca ccatcgtcaa
     2641 ccggccgggc ggcgtagtcg tcatccacgc gcgggatgac ctcggcaacc cgatcaatga
     2701 catctgcgtg gcgacatacc aggatgccgg tggcggcgcg ctcggcacgt tcatggggct
     2761 gccgtgtgtg cagggggatt ccgcgaccga cggcgagagc cgcatcatcg gcttcccgac
     2821 cggcaactat gttctcatcc aattgaccag tcagtccggg tatgtccggg ccgcgccgaa
     2881 gcggatctcg gtcaccgagg gcacgacgac cacgatcacc atcgtgaacc cgctgggcgg
     2941 ggcgctgacg gttcaccacc aggacaatac ggggaccgcg gtcgtcgatg ggtgcttccg
     3001 ggtcgtcaag gacgccggcg gcgggacgcg gggcgaggcc gtcggcgatt ccgtctgcga
     3061 tgcgtacgat tcggcccggg atggcgacac gcacatcacc ggcctgccct caggcagcta
     3121 cctgctcgtc gacctctcct tctcgccgac gcgctccaac agcgcggaca ttcccttcac
     3181 gatcgcgacg cgccagacga aggagttgac cgtcaccagc gtcaaccacg gcaccttgcg
     3241 cctggctcgg accgacgccg gcggtgccac ggtgctgggc ggctgtgtcg acgtgttctg
     3301 ggcggcgggg acgccgaatg ccgggcaaca ggtcatcttc aacgcctgcg acggcctccg
     3361 cgacgtggcg gacggcacgc tcgagctgcg gctgccgaac ggcagattcg aggtgcgcga
     3421 tggcaatctc ccattgggat acgtgcaggg agacccgatc acggtcgaga tcatcgacag
     3481 cggtctgacc gaagccacca tcgtcaccaa gcgaatgccc gacctgaccg tccgggccgt
     3541 cgatacctgg ggcgacccgg tcaaggacgt ctgtgtcctg ctgttccctg acgagacaag
     3601 cggcaacacc gacatggagc aggccgtatg cgatagcgcg gatggcgcca ccgacggccg
     3661 tattctcttc cgtaccctgg tcgggccggg cgtgtggctg gtccgctcga ccccggacag
     3721 cagctacccc ttcgccgcga cctcgatcac cctcgccgcg gacgaggttc gcacgctgga
     3781 tgtcatcgtg accatgccgg ccccgaagat cctggtcggg ccgctcctga tggacattac
     3841 cgacacctcg gccaccgtct acttcaccac cgaccagaag accgtcagca ccgtggcgtt
     3901 cggtaccggc gatccctttg atcaatccgc cccggcgacc gcggagacgc cgagaaagac
     3961 gcaccggatc cagatcaccg ggctgcagcc caacaccatc tacgactggt cgctgaccac
     4021 cgggaacgga tatggatcgg cgaacgccga tgggtggcgg ttccgaaccg gggcatcgaa
     4081 cgcgcagatc gtgatcaaga aggtggactc ggcggggagt accctgaagg gcgcctgctt
     4141 cgagctctac accgacgcgg gcaatggcgc cctcggcacg tacatcatgt ttgagtgcga
     4201 cgcgttcgaa gccgacggga gcaacggcct catcaccttc ggcgggctcg atccgggcag
     4261 ctacgtcgtg accgaaatgt ccgcgcccag caagttcaag ctcgcggcgc cggtccgcac
     4321 cacgttgacc gccggccaga tcaagcagct gacagtcacg gattaccggg gcggtaccgt
     4381 cgtcaagatc cggaacgaag cctacggcca tgagttgctg cccgggtcgt gcttctacgt
     4441 ctacaagcgc ggctccggcg aatcgctcgg cgcctatgtc acctggagct gcgacgagtt
     4501 cgatgggacg gacgcctcca ccaccgtgag cgggttgccc gtcggcaact acgtgctcgt
     4561 ccagtcctac gtgccggacg gcttcgtcga ggcggagtac atgtccttct cgatcaccag
     4621 cagtaccggg agcagcctga gcctgaccgt ggtcaaccat cagaccaatg gcgagcaggt
     4681 cgttgccgtt cgcacggtga atgccgccgg caccctga
//
LOCUS       DASSGX010000439         2290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_403331, whole genome shotgun sequence.
ACCESSION   DASSGX010000439 DASSGX010000000
VERSION     DASSGX010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403331"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="VFI12_07835"
     CDS             <1..647
                     /locus_tag="VFI12_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HET7056359.1"
                     /translation="PRFTPPPPDVKLSDLERIVEFVRQPERDENPARQLRAFKVRAMI
                     LMMFRTGVRVSELCAFRRRDIELSDGQAHVYRAKGGKSRTVHFDAETAQALQAYWNLR
                     GDAIRGAGAAPAFSGRDKVGDAGNPISPRTVEHIISQLCTRLGIESEITPHSFRHGLA
                     TEMVRRRVRESTVQTILGHASPQTTRIYVHKSAVEVADEYQDAFGQYRAPAGAD"
     gene            700..1230
                     /locus_tag="VFI12_07840"
     CDS             700..1230
                     /locus_tag="VFI12_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056360.1"
                     /translation="MLLAACSTGNADSANEAATREAKRATNVVADTEATQVVREFFDQ
                     KQTPTPFPTQRPTLANLRLTTTLREEDAPTNTVYSYTRGSGPLYADAQIGNVFPGQRV
                     IAVWSMGGTVMQTTEVNVDNPHELIWIALRWDIPPSAPAGQYTVAIQVAGPGTNDEGT
                     PADVTTEIGTLVFRVN"
     gene            complement(1243..2097)
                     /locus_tag="VFI12_07845"
     CDS             complement(1243..2097)
                     /locus_tag="VFI12_07845"
                     /inference="COORDINATES: protein motif:HMM:NF013899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HET7056361.1"
                     /translation="MRASACLTVARSSRDDRSRITRLRSDPPLVLRSTNPDGPEPPRS
                     WELPGRMPVRVSLAAGAAGPVGGDDLRLAIDVERGAALVLRTVAATLALPGPHGLPSR
                     SEVVVRVAEDGILSWLPGPLIAARGCDHHEAITVALGPGARLLLREELLLGRHGEEPG
                     AIRRRLRVCLGDQPVLDQELALGPETPGWDGPAVTGGRRAIGSLLLVDPCCERESFRT
                     QLPAPSGGTAFLPLHGPAVLVIALATDSLELRSQLNTALHAIESALSPARGQGPCLAH
                     ASKRATIL"
     gene            complement(2097..>2290)
                     /locus_tag="VFI12_07850"
     CDS             complement(2097..>2290)
                     /locus_tag="VFI12_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET7056362.1"
                     /translation="TADLLVINKTDLADRVGADLGVMERDARARRGGLPMLFSSLTED
                     PAAANVAEWVRDRLAVPAA"
BASE COUNT          403 a          787 c          782 g          318 t
ORIGIN      
        1 tgccgcgctt cacgccgccg ccacccgatg tcaagctgtc cgacctggag cggatcgtcg
       61 agttcgtccg ccagccggag cgggatgaga acccggccag gcagcttcgg gcgttcaagg
      121 tccgggcgat gatcctgatg atgttccgga cgggcgtgcg cgtttccgag ctctgcgcgt
      181 tccggcggcg ggatatcgag ctgtcggacg ggcaggccca cgtttaccgg gccaagggcg
      241 gcaagagccg gaccgtccat ttcgatgccg aaacggcgca ggcgcttcag gcgtactgga
      301 acttgcgagg cgacgcgatt cgtggcgcgg gcgctgcgcc ggccttcagc gggcgggaca
      361 aagtcggcga cgccggcaac ccgatcagcc cgcgcacggt tgagcacatc atcagccagc
      421 tctgcacgag acttggcatc gagagcgaaa tcacgccaca ctccttccgg cacgggctcg
      481 ccacggaaat ggtccggcgc cgggtacgcg aatcgacggt acagacgatc cttggccacg
      541 catcaccaca aacaacccgc atctacgtcc acaagtcggc cgtcgaagtc gccgacgagt
      601 accaggacgc cttcggccag taccgggctc cggccggggc cgattaggcg ggacgcgcgc
      661 cgctggtttg gcgtgctcct ggccggatgc tggtgcgtcg tgctgctcgc ggcctgctcc
      721 accggcaacg cggatagcgc aaacgaggcc gcgacccgcg aagcgaagcg agcgaccaac
      781 gtggtggccg acaccgaggc gactcaggtc gtccgcgagt tcttcgacca gaagcaaacg
      841 cccacgccat ttccgaccca gcggccgacg ctggccaact tgcggctgac aacgaccttg
      901 cgcgaggaag atgcgccaac caatacggtc tacagctaca cccgcggttc cggcccgctc
      961 tatgccgatg cgcagatcgg caacgtcttc cctggtcagc gcgtcatcgc cgtctggagc
     1021 atgggcggta cggtgatgca gacgaccgag gtcaacgtcg acaatccgca tgagctgatc
     1081 tggatcgcgc tccgctggga catcccgccc tccgcgcccg caggccagta caccgtcgct
     1141 atccaggtcg ccggaccggg taccaacgac gagggcacgc cggccgacgt gaccaccgag
     1201 atcggcaccc tcgtcttccg cgtcaactga cctggaacgt catcacaaga tagttgcacg
     1261 cttcgaagcg tgcgcgaggc aagggccctg gccccgggct ggcgacagcg ccgattcgat
     1321 cgcgtgcagg gcggtgttca gttgactccg gagctcaaga ctgtccgtgg ccagcgcgat
     1381 cacgaggact gccgggccat ggagcggcaa gaacgcggtt ccgccgctcg gagccggtag
     1441 ctgcgttctg aatgactcac gctcgcagca gggatcaacg aggaggagcg aaccaatcgc
     1501 ccttcgcccc ccggtgacgg ctggaccgtc ccaccccggg gtttcaggac cgagggcgag
     1561 ctcttgatcc agcacgggtt ggtcgccgag gcagacccgg agccgccggc gaatcgcgcc
     1621 cggctcttcg ccatggcggc cgagcaggag ctcctcgcgc aggagcaggc gcgccccggg
     1681 cccaagcgcg acggtgatcg cctcgtggtg atcgcaaccg cgcgcggcga tcagggggcc
     1741 aggtagccag gacaggatgc cgtcttcggc gacccgcacg acaacctcgc tccgggaagg
     1801 cagaccgtgc gggccgggca gagcaagggt agccgccacg gttcgcagca caagcgccgc
     1861 gccgcgctcc acatcaatcg ccagtcgcag atcgtccccg ccgaccggac cggccgctcc
     1921 ggccgcgaga gagacccgga cgggcatccg gccgggaagc tcccaggagc gcggcggctc
     1981 ggggccatcc gggttcgtcg accgcaggac gagcggtgga tcggagcgaa ggcgcgtgat
     2041 ccgggaccgg tcatcacgcg acgatcgcgc aacggtcagg caggccgagg cgcgcatcag
     2101 gcggccggga cggccaggcg atcacggacc cattccgcca cgtttgccgc ggcggggtcc
     2161 tctgtgaggg agctgaagag catcggcaag ccgccccgtc gtgccctggc gtctcgctcc
     2221 attacgccga gatcggcgcc gacgcgatct gcgaggtccg tcttgttgat aaccagcagg
     2281 tcggccgtgg
//
LOCUS       DASSGX010000440         5108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_76772, whole genome shotgun sequence.
ACCESSION   DASSGX010000440 DASSGX010000000
VERSION     DASSGX010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5108
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76772"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1514)
                     /locus_tag="VFI12_07855"
     CDS             complement(<1..1514)
                     /locus_tag="VFI12_07855"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HET7056363.1"
                     /translation="MAADRVLVNARVLTMAGGSVAEALAIAGGRIVAVGSDAEVRAMA
                     GPETVVEDLGGAVVLPGLIDAHNHLLMTGQILQQVQLYDCRSIDDILDRVRAAVETSP
                     QGTWILGRGWDESLLAERRHPTRHDLDRVAPEHPVVIHRVWNRLVANSRAIELAGISR
                     ETPDPPADQPYAGGFDRDADGEPTGLFRDRAKELITSAVPAPTVDQLVSAIKTACQEY
                     NRLGITAVSEPGLSPDQIRAFHEAELRGDLTVRTAMSIAGWGFGTQSEDGKIRDRLAG
                     VGVMGGFGDDLLWIDGVKLMPDGGAGDRTARMYQPYEGEPDNFGTWTVPPGELVEHIR
                     WAHDHGYAMDIHTCGDEAQDVAVQAFALAQERNPNPRLRHRVHHAYLPTEQALETMAK
                     HQIPAVVSNPFLWSLGESYLTSIGPERAARMMPMRTYLNRGVPLAGSSDSPVATHNPW
                     VGMASAITRTTVQGTELGPDERLAPEEALRMYTAGGAFVLHRETTLGTLEPGKLA"
     gene            complement(1603..2385)
                     /locus_tag="VFI12_07860"
     CDS             complement(1603..2385)
                     /locus_tag="VFI12_07860"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HET7056364.1"
                     /translation="MASESQGMLAGKNILIMGVANRWSIAYAIGEAMKAQGATLILSY
                     LDERMKKDCDVLLADQPGGKMYPCDVSKDEDIDALAESIKQDFGRLDGYIHSVGFAPG
                     DALKNRFVETSRDSWRIALDVSAYSLVAVSARMAPLMTDGGSIQTLTYLGSERVFPAY
                     KVMGVAKAALEASVRYLAWDLGEQGIRVNAISAGPIKSAAARGIPGFADMYGAMLERA
                     PLKDEFGAAQVASVAVFLASDYSSAITGEVIFADNGYHAMGM"
     gene            2453..3520
                     /locus_tag="VFI12_07865"
     CDS             2453..3520
                     /locus_tag="VFI12_07865"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HET7056365.1"
                     /translation="MSDQQRIYPVFDGHNDTITHLREAGASFFEENADFHITLPLAKR
                     SGLIGGFFAIWVPDTNEVPNFTDPNTDSYRSVETMPPMMSTGHAQEFALVSLGRLLKL
                     ERESEGAVAIVRSVGDIERAMDRGQFSIEVHIEGAEPIDPALDTLDAWYAAGLRSLGL
                     VWSRPNAFGHGVPFDFPSSPDTGPGLTDAGKALVRACNQLGIMLDISHLNEKGFWDLA
                     KISDRPIVATHCGAHALCPSPRNLTDKQLDAIRDSDGIVGVNFHVGFLNPLGNEATAE
                     NTSLTAIADHLDYIAERIGIERVALGSDFDGARMPGDLESAGGLQKLMQELKSRGYDD
                     AALAKIGYENWLRVFRATWVN"
     gene            complement(3771..3980)
                     /gene="rpsR"
                     /locus_tag="VFI12_07870"
                     /pseudo
     CDS             complement(3771..3980)
                     /gene="rpsR"
                     /locus_tag="VFI12_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005705387.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
     gene            complement(4221..>5108)
                     /locus_tag="VFI12_07875"
     CDS             complement(4221..>5108)
                     /locus_tag="VFI12_07875"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HET7056366.1"
                     /translation="RRMKFVLHLNDFSWPQEPARFGPTLAEIARASESAGFDAIAVMD
                     HLWQHPMMDGPEKPVLECYSTLAYIAAHTERIGLMSLATAVSYRSVGLLAKMVTTLDV
                     LSGGRAWLGIGAGDYEEEATGLGVPYPSLDERFLMLEETLRACLQMWEGDHGADGPFT
                     GTHVQMERTLNLPQSIARPHPPILIAGSGEQRTLRLVAQYGDACNIRPGPEIPHKLEV
                     LKRHCESVGRNYDEIERTCPFLFDVGEDGAKADALIEQLRGLARLGIQKVFGRVVGDY
                     RIYPIEIMGRDVIPTVATL"
BASE COUNT          855 a         1697 c         1646 g          910 t
ORIGIN      
        1 gcgagcttgc cgggttcgag cgtgccgagc gttgtctcac ggtgcaggac gaaggcgcct
       61 ccggcggtgt acatccgcag cgcttcctcg ggcgcgagcc gctcgtcggg accgagctcg
      121 gtgccctgca cggttgttcg cgtgatggcg ctggccatgc cgacccacgg gttgtgggtc
      181 gcgacgggcg aatcggacga tccggccagc ggcacgcccc ggttcaggta cgtccgcatc
      241 ggcatcatcc gcgccgcccg ttccggaccg atcgacgtca ggtagctctc tccaagcgac
      301 cagaggaacg ggttggagac gacagccgga atctggtgct tcgccatcgt ttccagcgcc
      361 tgctcggtcg gcaggtatgc gtgatggacg cggtggcgga gccggggatt cgggttgcgc
      421 tcctgcgcca gggcaaaggc ctgaaccgcg acgtcctgcg cctcgtcacc gcaggtgtgg
      481 atgtccatcg cgtagccatg atcgtgcgcc caccggatgt gctcgaccag ttcgcccggc
      541 gggacagtcc acgtgccgaa gttgtccggc tcgccctcat acggctggta catgcgggcg
      601 gtgcgatcac ccgcgccgcc gtcaggcatc agcttgacgc cgtcgatcca gagcagatcg
      661 tcgccgaagc cgcccatgac gccgacgccg gccaggcggt ctcgaatctt gccgtcctcg
      721 ctctgcgtcc cgaatcccca tccagcgatc gacattgcgg tgcggacggt gaggtcaccc
      781 cgcagctcgg cctcgtgaaa ggcgcggatc tggtccggcg acaatcccgg ttccgagacg
      841 gcggtaattc cgagccggtt gtactcctgg caggcagtct tgatcgcgga taccagctgg
      901 tcgacggtgg gcgcggggac tgccgaggtg atcagctcct tggcgcgatc ccggaagagg
      961 ccggtcggct cgccgtcggc gtcccgatcg aacccaccgg catagggctg atcggcgggg
     1021 ggatcgggcg tctcgcggga gatcccggcc agctcgatcg cgcgggagtt ggcgaccagc
     1081 cggttccaga cgcggtggat cacgaccgga tgctccggcg cgacccgatc gaggtcgtgc
     1141 cgcgtcgggt ggcgacgctc ggccaacagg ctctcgtccc atccccgtcc gaggatccac
     1201 gtcccttgcg gcgacgtctc gacggcggcc cggacccgat caaggatatc gtcgatgctg
     1261 cggcagtcgt agagttgaac ctgctgcagg atctgcccgg tcatcagcag gtggttgtgg
     1321 gcatcgatca acccgggcag caccacggcg ccgcccaaat cctcgaccac cgtctccgga
     1381 ccggccatgg cccggacctc ggcatcggaa ccaaccgcca cgatccggcc gcctgcgatc
     1441 gccagcgcct ctgcaaccga gccgcccgcc atcgtcagaa cacgcgcatt caccaggacg
     1501 cgatcagccg ccattggtct ctcgtctttc gtctcttgtc gttcgttctc ggttttccgc
     1561 tgtccgtact gacgtctgaa gcccgacgtc tggcgtctaa agtcacatgc ccatggcgtg
     1621 gtagccgttg tcggcgaaga tgacctcgcc ggtgatcgca ctggagtagt cgctggccag
     1681 gaagacggcg acgctcgcca cctgcgccgc gccgaactcg tccttcagtg gggcgcgctc
     1741 gagcatggcg ccgtacatgt cggcgaagcc ggggatgccc cgcgccgccg ccgacttgat
     1801 cggcccggcc gaaattgcgt tgacccggat cccctgctcg cccaaatccc aggccaggta
     1861 gcggaccgac gcctcgagcg ccgccttggc cacgcccatc accttgtagg ccgggaagac
     1921 gcgctccgaa ccgaggtagg tcaacgtctg gatgctgcca ccgtcggtca tcagcggggc
     1981 cattctggcc gagaccgcta ccaatgaata ggcgctgaca tcgagggcga tgcgccagct
     2041 atcgcgagag gtctcgacga accggttctt cagggcatcg ccgggcgcga agccgacgct
     2101 gtggatgtag ccatcgagcc ggccgaaatc ctgcttgatc gactccgcca gggcatcgat
     2161 atcctcgtcc ttcgagacat cgcagggata catcttgccg cccggctggt cggccaggag
     2221 cacgtcgcaa tccttcttca tgcgctcgtc gaggtagctg aggatgagcg tggcgccctg
     2281 cgccttcatc gcctcgccga tggcataggc gatgctccag cggttggcga cgcccatgat
     2341 caggatgttc ttgccggcca gcatgccctg cgattcagat gccattcagt gatcccctcc
     2401 gctgcggtag agtgacggcg ttcggcagat gatactcgtc cgaggtattc acttgagcga
     2461 tcagcagcgt atctacccgg tattcgacgg ccacaacgac acgatcacgc acctgcgcga
     2521 ggcaggcgca tccttcttcg aggagaacgc ggactttcac attaccttgc cactggcgaa
     2581 gcgaagcggg ctgatcggcg gtttcttcgc gatctgggtg ccggacacga acgaagtccc
     2641 gaacttcacc gatccgaaca ccgactccta ccgcagcgtc gagacgatgc cgccgatgat
     2701 gtcgaccggt catgcccagg agttcgcgct tgtctcgctc ggacgtctgc tcaagctgga
     2761 gcgcgagagc gagggcgcgg tggcgatcgt gcgctcggtc ggcgacatcg agcgggcaat
     2821 ggaccgtggt caattctcga tcgaggtcca catcgagggc gcggagccga tcgacccggc
     2881 gcttgatacg ctcgatgcct ggtacgccgc cggattgcgc tcgctcgggc tcgtctggtc
     2941 gcgcccgaac gcattcggcc atggcgtgcc atttgatttc ccctcctcgc ccgataccgg
     3001 accgggcctg accgatgccg gcaaggcgct ggtccgcgcc tgcaaccaac tcggcatcat
     3061 gctcgacatc tcgcacctca acgagaaggg cttctgggac ctggccaaga tcagcgaccg
     3121 cccgatcgtg gcgacccact gcggcgcgca cgcgctctgc ccctcgcccc gcaacctgac
     3181 tgacaagcag ctcgacgcca tccgggattc ggacgggatc gtgggcgtca acttccacgt
     3241 cggcttcctg aacccgctcg ggaacgaggc cacggccgag aacacgtcgc tgacggcgat
     3301 cgcggaccac ctcgattaca tcgcggaacg gatcgggatc gagcgggtcg cgctcggctc
     3361 ggatttcgac ggggcgcgga tgccgggcga cctcgagagc gccggtggct tgcagaagct
     3421 gatgcaggag ctgaagtcgc gcggctacga cgatgcggcg ctggcgaaga tcggctacga
     3481 gaactggctc cgcgtcttcc gcgctacgtg ggtaaattag gcgcgcgggg gcgagtgaag
     3541 gcgtagcgcg gagttaccat gccccgcccg aaccccatcc cgtttacctc gttggaggga
     3601 accaatccag ttcccggtat tggctctgtc taccggacga gtggatcgac gatgcggggc
     3661 acggtgaccc cgcgctacga cattgattcc cgacaaagag cctgaagggc gcaagaaaac
     3721 gggctccgga ttgctccgga gcccgtttcg ggttcatatc aaccggccgg ttagcggcgg
     3781 cggtcgctcg gcacgaagtg caggagggcg aggtggcggg cccgcttgat cgcggtcgtc
     3841 agctcacgct gcttgcctgc ggagcaatgg gtcttgcggc gcggcagaat cttgccccgc
     3901 tcatcaaggc acatcttgag acgctggacg tccttgtagt cgaccacctc gttgtcggcg
     3961 aagaccggtt gcttgcggcg accggttccg cggcctccgc gcggaccgtc ccggcgatct
     4021 cgtggtggtc gagacggacg ttccccagcg gcgccttgtc cgaagcgcga gcgctgctct
     4081 gtcggagcgc tctcggtcgc ctggttcgtc tgctcgttat ccatacctgt aaacgtttct
     4141 ccttgtgcgt gcgcacgata accaatggat ggtagcacag ccggccggat gcatcacctc
     4201 cggaggtcat gcctgcgccg tcacagggta gcgaccgtgg gtatcacatc gcggcccatg
     4261 atctcgatcg ggtagatccg gtaatctccc acgacccgcc cgaagacctt ctggatgccg
     4321 agtcgggcca ggccgcgcaa ttgctcgatc agggcatcag cctttgcgcc atcctcgccc
     4381 acatcgaaca ggaacggaca cgtccgttcg atctcgtcgt aattccgccc cacggactcg
     4441 cagtggcgct tgagcacctc cagcttgtgc ggaatctcgg gacccggccg gatattgcag
     4501 gcatccccat actgcgcgac aagccgcaat gtccgctgct cgccgctccc ggcgatcagg
     4561 atcggtgggt gtgggcgcgc gatgctctgg ggtagattga gcgtccgctc catctggaca
     4621 tgtgtcccgg taaatggccc gtccgccccg tggtcccctt cccacatctg gagacaagcg
     4681 cggagcgtct cttcaagcat caagaaccgc tcgtcgagcg atgggtacgg aacacccagc
     4741 ccggtcgcct cttcctcata gtcccccgcc ccaatgccga gccacgcgcg cccgccggag
     4801 agcacgtcaa gcgtggtgac catctttgcc aacaagccga ccgaccggta ggagacggca
     4861 gtggcgaggg acataaggcc gatgcgctcg gtatgcgccg cgatgtacgc aagcgtcgaa
     4921 tagcactcga ggaccggctt ttccggtcca tccatcatcg ggtgctgcca gagatgatcc
     4981 atcacggcga ttgcatcgaa gccggccgat tcgcttgccc gggcgatttc cgcgagtgtt
     5041 ggaccgaatc gggccggctc ttgcggccag gaaaagtcgt tgaggtgcaa aacgaatttc
     5101 atccggcg
//
LOCUS       DASSGX010000441         2121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1273821, whole genome shotgun sequence.
ACCESSION   DASSGX010000441 DASSGX010000000
VERSION     DASSGX010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1273821"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VFI12_07880"
     CDS             <1..481
                     /locus_tag="VFI12_07880"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET7056367.1"
                     /translation="TMDNFLLTMNISVYSFIAVAQRARAMMKPGGSMLTLTYYGAEKV
                     IPHYNVMGVAKAALESSVKYLAADLGPEGIRVNGISAGPIKTLAASGIGDFRYIMKWN
                     EYNAPLRRNVTIEDVGGAGLYLLSDLASGVTGEIHHVDAGYNVVGMKAEDAPDIATV"
     gene            486..983
                     /locus_tag="VFI12_07885"
     CDS             486..983
                     /locus_tag="VFI12_07885"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HET7056368.1"
                     /translation="MIRPATPEDAAGIRHVHLSAFPTPAEADLVQELERAGDAVVSIV
                     AEDEGIVGHVLFSRMEVLADGRPLAALGLAPVAVVQGRQSEGIGGALIEAGLSRASEL
                     GIDIVFLLGEPGYYRRFGFEASAAEPFASPYAGPYFQALALAGDYARPDSGTAGYAPA
                     FAGFE"
     gene            1004..1279
                     /locus_tag="VFI12_07890"
     CDS             1004..1279
                     /locus_tag="VFI12_07890"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HET7056369.1"
                     /translation="MSGRVQGVFFRAWAQGQARELGLSGWIRNCPDGSVEAHLDGDEN
                     AVERMIERMRRGPSDARVEDVAVEEAEPEDTGRFELRTSPRTGPSPE"
     gene            complement(1248..2102)
                     /locus_tag="VFI12_07895"
     CDS             complement(1248..2102)
                     /locus_tag="VFI12_07895"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF012912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HET7056370.1"
                     /translation="MRVCDLARLDDLGPLLDRLCAHHDALRAGFGEPPADRERLRNDL
                     AEIAEFVLPFAKPVWRDLDAAFRRGRRILFEGAQGVLLDVDHGTYPFVTSSNTVAGAT
                     GPGTGMGPSAAGFVLGIVKAYTTRVGSGPFPTELDDEVGQRLGERGHEFGTVTGRQRR
                     CGWFDAVLVRQSVAVSGVTGVALTKLDVLDGFETVKICTGYRIDGKKFDYLPPHAQDQ
                     ARVEPVYEEIEGWPGSTRGARSWADLPARAIKYVRRIEELIRCPVALVSTSPERDDTI
                     LVRDPFAG"
BASE COUNT          342 a          692 c          739 g          348 t
ORIGIN      
        1 gaccatggac aacttcctgc tgaccatgaa catctcggtc tacagcttca ttgccgtcgc
       61 ccagcgcgcc cgcgcgatga tgaagccggg cgggtcgatg ctgaccttga cctattacgg
      121 cgcggagaag gtcatcccgc actacaacgt catgggcgtc gccaaggccg cgctcgaatc
      181 gtcggtgaaa tatctcgcgg cggatctggg tcccgagggc atccgggtga acggcatctc
      241 cgcgggaccg atcaagacgc tggcggccag cggcatcggc gacttccgct acatcatgaa
      301 gtggaacgaa tacaatgcgc cgcttcgccg gaatgtgacc atcgaggacg tcggcggggc
      361 aggcctctat ctgctctccg atctagcgag cggagtcacc ggcgagatcc accatgtcga
      421 tgccggctat aacgtcgtcg gaatgaaggc cgaggacgcg ccggacattg cgaccgtgtg
      481 atgtggtgat ccgccctgca actcccgagg acgcggccgg aatccgtcac gtccatctta
      541 gcgcctttcc gacgcctgcg gaggcagacc tcgttcagga gctcgagcgg gccggagacg
      601 ccgtcgtctc gatcgtcgcc gaggatgagg gcatcgtcgg ccatgtgctc ttcagccgca
      661 tggaggtgct ggccgatggc cgcccgcttg ccgcgctcgg cctcgcaccg gtcgccgttg
      721 tgcagggccg ccagtcggaa gggatcggtg gagccctgat cgaagctggc ctgagccggg
      781 cgagcgagct gggcatcgac atcgtcttcc ttctgggaga gccgggctat tatcggcggt
      841 tcggtttcga ggcgtctgcg gcggagccgt tcgcttcgcc ctatgcgggt ccatacttcc
      901 aggcgctggc gctagccggt gactatgcgc ggccggactc aggaacggcc ggctatgctc
      961 cggccttcgc agggttcgaa tgatcatcgc ccgccatgtc cgagtgagcg gacgcgtcca
     1021 gggcgtcttc ttccgtgcct gggcacaagg tcaggcgcgc gagctcgggc taagcggctg
     1081 gatccgcaac tgtcccgacg gctcggtgga ggcgcatctc gacggggacg aaaatgcagt
     1141 cgagcggatg atcgaacgga tgcgccgcgg cccttcggat gcgcgcgtcg aagatgttgc
     1201 ggtagaggag gcggagcccg aagacaccgg ccgcttcgag cttcgcacta gcccgcgaac
     1261 gggtccctca ccagaatagt atcgtcgcgc tcgggcgagg tgctgaccag cgccaccggg
     1321 cagcggatca gctcttcgat gcggcgcaca tatttgatcg cccgcgccgg aaggtcggcc
     1381 cagctgcgtg cgcctcgggt cgagcccggc cagccttcga tctcctcgta gaccggctcc
     1441 acccgagcct ggtcctgcgc atgcggcggc agatagtcga acttctttcc gtcgatccgg
     1501 tagccggtgc agatcttcac cgtctcgaag ccgtcgagga cgtccagctt ggtcagggcc
     1561 acgcccgtga ccccgctgac ggcgaccgac tggcggacca gcacggcgtc gaaccagccg
     1621 catcgccgct gccggccggt gacggtcccg aattcgtgcc cgcgctcgcc gagccgctgg
     1681 ccgacctcat cgtccagctc ggtcgggaag gggccggagc cgacgcgcgt cgtgtaggcc
     1741 ttgacgatgc cgagcacgaa gccggccgcc gacgggccca tgccggtgcc ggggccggtt
     1801 gctccggcaa cggtgttgga ggacgtgacg aacggatagg tgccgtgatc gacgtccagg
     1861 agcacgccct gcgcgccttc gaacaggatt cgcctgcccc tgcggaaggc ggcgtcgagg
     1921 tcgcgccaga cgggctttgc gaacggcagg acgaactcgg cgatctcggc cagatcgttc
     1981 cggagccgct cgcggtcggc cggaggctcg ccgaagccgg cgcgcagggc gtcatggtgc
     2041 gcgcagaggc gatcgagcag aggcccgagg tcgtcgagac gggcgaggtc gcagacgcgg
     2101 atcgctcggc gcccgacctt g
//
LOCUS       DASSGX010000442         4147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_512692, whole genome shotgun sequence.
ACCESSION   DASSGX010000442 DASSGX010000000
VERSION     DASSGX010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512692"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..574
                     /locus_tag="VFI12_07900"
     CDS             161..574
                     /locus_tag="VFI12_07900"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7056371.1"
                     /translation="MVNHSERTGCVVPVGDKYRGKQGLDYVPAVSAENVGSHGLWFGT
                     MTIPPGGRTAAHFHEHHETAIYVISGDEVEMYSGEQLEVMEICRPGDFLYIPAGLPHV
                     AVNRSQTPAFVVAARTDPNEQESVVMRPDLDSRAP"
     gene            825..1760
                     /locus_tag="VFI12_07905"
     CDS             825..1760
                     /locus_tag="VFI12_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056372.1"
                     /translation="MRKFGLGSCAIALLVLIAGMTLPGSVAAAPISISEGSGGLYSYQ
                     IIVAGPFAPGTGVNYWTVDGTATGADGDFEAIPINGASALCPSAGNPCPTVNLYVTIN
                     GDTTPEPDEMFSVVTSNALTYEITLLNDDGPVVATNTPTNTPTNTPTDTPTNTPTNTP
                     TSTPTDTPTNTPTSTPTDTPTSTPTNTPTSTPTDTPTATPTNTATATPTDTPTNTPTN
                     TATPTETSTPTATATPTIDQTVAAATQAALQTATARAEATGTAAGDTTGDVTDLPNTG
                     SGPDGTSTGAGMALFGIVIAAGAALTLQRRRMQRG"
     gene            complement(1836..3044)
                     /locus_tag="VFI12_07910"
     CDS             complement(1836..3044)
                     /locus_tag="VFI12_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056373.1"
                     /translation="MGCGCQDQNAKTDDFCPDARDASTDVAEVTTKESLQRRTLLKAA
                     AVGSALAALAGRESLTPFSAMAHTDTKSACTAQDIDVSSGTIVNEPCTCAAGGTFQAI
                     AAFQVTNNNNAARKCITLHMGAGGTFGGQDFLLVDANGNSNIDGKGTTKTMFATLGSV
                     NCNFGQECYPGSVVAFQTAQNQSDTACPGPLTKYPGGQCRRQEICIVGFGATLACASD
                     GTCSSTGSGNCSIACGGNLFLKASATGGTAGSNGTYTFTVKNASGTTVASAPAGSSSP
                     QCLTVSSPTAGTYTLTVTDSKGCTRTASVTVAVSSITAGLAGTAGGCDGTVDFTASTT
                     GSGSCTYEFVVAGQSQQGPGSSNTFTYDPVALDHVDGTDHSVRVHVVCGSCSADATKT
                     VNTCATTTVS"
     gene            complement(3753..>4147)
                     /locus_tag="VFI12_07915"
     CDS             complement(3753..>4147)
                     /locus_tag="VFI12_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056374.1"
                     /translation="KASDGLFRSPAGLAGCSVNVKYRSSSSRRLNIENSAELRNHPDY
                     YLAFRGPKLPSGPASEKTLPLVIDAVYLFCSAELIPALVAEGVANIGPSVKSLYWQAA
                     MIYPEQVNRALILSQEQRAALALFAPLG"
BASE COUNT          790 a         1326 c         1301 g          730 t
ORIGIN      
        1 tggccgaagg ccacgatggt tctttctgcg gccaggctcc gaggaccgaa aggagatggg
       61 tcgggaacga tcatcgccct gcgggcgatc gcggggcgtg gtaaccccgc gctacgatag
      121 ccgtcacaca gcaggcggca atgagcgagg agcactcgtg gtggtgaacc attccgaacg
      181 caccggttgc gtcgtcccag tcggagataa gtaccgcggc aagcaggggc tggattacgt
      241 gccggcggtc agcgctgaga atgtcggctc gcacggactc tggttcggca cgatgacgat
      301 cccgcccggt ggccgcaccg ccgctcattt ccacgagcac cacgagaccg ctatctacgt
      361 gatcagcggc gacgaggtcg agatgtactc cggcgagcag ctcgaagtga tggagatctg
      421 ccgccccggc gacttcctct acattccggc cggcctcccg cacgtcgccg tcaaccggag
      481 ccagacaccc gccttcgttg tcgccgcgcg gaccgatccc aacgagcagg aaagcgtcgt
      541 catgcggccg gacctcgatt cgcgcgcccc ctgaaatcgc ccgccgcgct acccggtatc
      601 cccagaacga ggctccggga tcaggacctc tggtgtcggc tgtccttggt tgcgtagccg
      661 gctgttctgt agcctgaata tcatatagac tgataccccg caccgttcat ctcctcctat
      721 tcagtcgcca ggacgctgcg tcgtcgccga tcgtggcggc gattcgccag gtatccggca
      781 cgccggcagc cgtcgcgtgc cagggaagca gggagtgcgc gtttatgcgg aagttcggct
      841 tggggtcgtg cgcaattgcg ctgctagtgc tgattgccgg gatgacgttg cccgggtcgg
      901 tagcggcggc gcccatctcg atatcggaag gcagcggcgg gctgtattcc tatcagatta
      961 ttgtcgccgg gccctttgcg ccgggcaccg gagtgaatta ctggacggtc gacggtaccg
     1021 cgaccggcgc ggatggcgac tttgaggcca ttccgatcaa cggcgccagc gccctctgtc
     1081 cctccgccgg aaatccgtgc ccaactgtca acctgtacgt caccattaac ggagacacca
     1141 ctcccgagcc tgacgagatg ttctcagttg tcacgagcaa cgcgctcacg tatgaaataa
     1201 cgctgctcaa cgacgacggg ccggttgttg cgacaaacac gccaaccaat acgccgacca
     1261 acactccaac cgataccccg accaatactc caacaaacac gccgaccagc actccgacag
     1321 acacgccaac caataccccg accagcactc cgacagatac gcccaccagt accccgacga
     1381 acacaccgac cagcacgcca actgataccc cgacggcgac gccaaccaat acggccaccg
     1441 caacgccgac ggatacgccc acgaacacac caaccaatac ggcgaccccg accgaaacca
     1501 gcacgccgac ggccacagcc acgccgacga tcgatcagac cgtcgccgct gccacccagg
     1561 ccgcactcca gaccgccacc gcacgcgctg aagcgaccgg caccgccgcc ggggacacta
     1621 ccggggacgt caccgacctc ccgaataccg gctcaggccc ggacgggacg tcgaccggcg
     1681 cgggaatggc cctattcggg atcgtgatcg ccgccggcgc ggccctcacg ctccagcgcc
     1741 gcaggatgca gcgcggctag cagccggcgc ggcatgaaac gaccggcgga ctcttgggtc
     1801 cgccggtcgt tcgtgtcacg gtgagtcggc tgagatcagc tcacggtggt cgtcgcgcag
     1861 gtgttcaccg tcttggtcgc atcggccgag cagctaccgc agacgacgtg aacacggacg
     1921 gagtgatccg tcccatcgac atgatcgagc gcgaccgggt catacgtgaa cgtgttcgac
     1981 gagcctggtc cctgttggga ttgcccggcg acgacgaact cgtatgtgca acttcccgat
     2041 cccgtcgtac tggcggtgaa gtccacggtt ccatcgcatc cgccggccgt tcctgccagg
     2101 ccggccgtga tcgagctgac cgccaccgtg accgaagcgg tccgcgtgca gcccttgctg
     2161 tcggtcacgg tcaacgtata ggttccggcc gttgggctgg agaccgtgag gcactgcggg
     2221 gatgatgatc ccgccggggc cgaagcgacg gtcgtgccgc tcgcattctt gaccgtgaag
     2281 gtgtacgtcc cgttcgaacc ggcggtgcct cccgttgcgg aggccttcag gaacaggttg
     2341 ccaccacagg cgatgctgca attccctgag cccgtgctcg agcacgttcc gtcgcttgcg
     2401 cacgcgaggg tcgcgccgaa accgacaatg cagatttcct gccggcggca ctggccgccc
     2461 ggatacttcg tgagcggccc tgggcacgcg gtatccgact ggttctgcgc ggtctggaac
     2521 gccacgacgg atcctgggta gcactcctgc ccgaagttgc agttcaccga tcccagagtg
     2581 gcgaacatcg tcttcgtagt gcccttgccg tcgatgttgc tgttgccgtt cgcatcgaca
     2641 agcaggaagt cctggccacc aaatgtcccg ccggcgccca tgtgcaaggt gatgcacttg
     2701 cgggcggcat tgttgttgtt ggtgacctgg aaggcagcaa tggcctggaa cgttccgccg
     2761 gccgcgcagg tgcacggctc gttgacgatc gtgcccgagc tgacatcgat gtcctgcgcc
     2821 gtgcaggcgg acttcgtgtc ggtgtgggcc atggccgaga agggggtgag actctcgcgg
     2881 ccggccagtg cagccagggc gctgcccacg gcggcggcct tcagcaaggt gcgacgctgg
     2941 agagattctt tggtcgtaac ctctgccaca tcggtggaag catcccgcgc atcgggacag
     3001 aagtcgtcgg tctttgcgtt ctggtcttga caaccgcaac ccatgatggg actcctttgc
     3061 tcaagcgatt ggttcggcga gccgatcgac ccgggtgttg gggcacgcgg gtaaaccttg
     3121 cggggcagtc cacgcggtcg ctgagcgaat ggggaatgga cccgcggcaa agggtggcga
     3181 gttcgcaatg gaggattgcg ccagtttgcg ctgagggtta gactggggat tcctccggcg
     3241 aactcattga gttcgtgcgg atgtacgggg cgggtttggc cgtatcgttc gcgcggtagc
     3301 caaactcgcc tcggtgtgtt catgcgctgg cggcgctcgg cacatccact ccataaagat
     3361 gacctcctga aaaacactca gacggcggag gcgaactggt tgtggttcgc ggactgcgcg
     3421 agcgaccggc cggtgtgccg gccacgggca ggcgtagcca gttgactgcc gaggaaaacc
     3481 tggaacgatg agcgaaccgg ggacaattcc tctcgtcagg taagaccgct caggatgggg
     3541 acctggtcgg ctgcggaaac gtttcgctgg ctaagatgat tgctgtgcgc agcctagtcc
     3601 cacgaccacc ataagtcaat cctcccaagt accgtagtta tatattcctg tacgttcgca
     3661 gtactaggta atttagccca aagagccaga taattagcca ggagagacaa ctcgtactgg
     3721 catgccgatg cgaagccaag agttccgggt tatcagccaa gtggggcaaa aagcgccagc
     3781 gcagctcgct gctcttgact gaggatgagc gcccggttca cttgctcggg atagatcatc
     3841 gccgcctgcc agtagagcga cttcactgag gggccaatgt tcgcgacgcc ctcggccacg
     3901 agggcaggga tcagctcggc cgagcaaaac aggtagaccg cgtcgatgac gaggggcagg
     3961 gtcttttcac ttgcaggtcc ggatggcaat ttcgggcccc ggaacgcgag gtagtagtcc
     4021 gggtgattgc ggagctccgc gctgttctcg atgttcaacc ggcgactgga gctgcttcgg
     4081 tacttcacgt tcaccgaaca acccgctagc ccagctggtg agcgaaacaa cccatccgat
     4141 gccttat
//
LOCUS       DASSGX010000443         2411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1495272, whole genome shotgun sequence.
ACCESSION   DASSGX010000443 DASSGX010000000
VERSION     DASSGX010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1495272"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1750)
                     /locus_tag="VFI12_07920"
     CDS             complement(<1..1750)
                     /locus_tag="VFI12_07920"
                     /inference="COORDINATES: protein motif:HMM:NF016232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding domain-containing protein"
                     /protein_id="HET7056375.1"
                     /translation="MSALPQRKEISISEPRRRARARTASQGAPDSHKPAPKQTDQWYR
                     ADLHIHTPGSIDYHDSGIRPIDLLRTAAEQSLDIIALTDHNSVAGWTRLKHEIEDLTY
                     LERLGRITDSESALLAEYRDLMSKVLVLPGFEFTATFGFHILAIFDPSTSTRLMEHLL
                     LSLGIPEDRFGSGEVGATSDVLKAYEVLAEHGALVVGAHVNSTHGVAMRNIRFGGQTK
                     IAYTQDENLHALEVTDLTSTSTRSTARFFSGTKAEYPRRMHCIQGSDAHRLKQDPTRA
                     SNLGIGDRVTEFLLPEKSFAALKDVLQSEEWDRVRPARGGGPQAAAVHAAREAGTSQS
                     IIFFERVQGGAKLSVGAVVRDVVALANGDGGTIYLGVGPASRRTVPGLPNADQLREEL
                     AAAFREQIEPPLAPEIEPITYENKPILAINVLAGTERPYALATGEILVRRGEESAPAR
                     RDEIVRMVRGEAVSEQPEPAPGQPETPAPERTSTAASERPKQERQDRQQSRSRQQRPQ
                     RQEKPSESGERIAPRNGVEIVEVSDQDGVPHYTMRDLRNDQVTRNVTAQTARSLWAQA
                     IREFEKGVPSEDRVTWD"
     gene            1845..>2411
                     /locus_tag="VFI12_07925"
     CDS             1845..>2411
                     /locus_tag="VFI12_07925"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HET7056376.1"
                     /translation="MTHTTADMMSSIGTPHVIDIAIVGAVVLFGAAGFWRGLNKELFI
                     TASLLLGYVVTLEWAPRWGRWLGDRFSALSTADGQYVAIAGTLLLSTLILGYISCTVA
                     ALPPADLPGRFGGLVLSAANATFAVTVLIARARQLVLDSSQQRTLRETRIGDWLSRNL
                     DWVVLALVVVGFLLLAGGAMTRRRRAALI"
BASE COUNT          363 a          745 c          806 g          497 t
ORIGIN      
        1 catcccaagt gacgcggtct tcgctcggca cgcccttctc gaactcgcga atcgcctggg
       61 cccagaggct tcgggcagtc tgcgccgtca cgttccgggt gacctggtcg ttgcggaggt
      121 cgcgcatcgt gtagtggggg acgccgtcct ggtccgagac ctcgacgatc tcgacgccat
      181 tgcgcggcgc gatccgttcg ccggattccg atggcttctc ctgccgctgg gggcgctgtt
      241 gccgtgagcg agactgctgt cgatcctggc gttcttgctt cggccgttcc gacgcggccg
      301 tggacgttcg ttccggcgca ggggtctccg gctgtccggg tgcgggctcg ggttgctccg
      361 agaccgcctc gccacggacc atccgcacga tttcgtcgcg gcgagcaggc gcggactctt
      421 ctccgcgtcg gaccaggatc tcgccggtcg ccagggcata gggtcgctcc gttccggcca
      481 acacattgat ggccaggatc ggcttgttct cgtaggtgat cggctcgatc tcgggcgcga
      541 gaggtggctc gatctgctcg cgaaacgcgg ctgcgagctc ctcccggagc tgatctgcgt
      601 ttggcaggcc cgggacggtg cggcgactgg ccggaccgac gcccaggtag attgtgccgc
      661 catcaccatt ggcgagggcc accacgtcac gcacgaccgc gccgacggac agcttcgcgc
      721 cgccctgcac gcgctcgaaa aagatgatcg actgcgacgt tccggcttcg cgagcggcat
      781 gcactgcggc ggcctgcgga ccgccgccac gggccggacg gactcgatcc cactcctcgc
      841 tctggagtac atccttgagg gccgcgaatg acttctcggg aagcaggaat tcggtgaccc
      901 gatcgcctat gccgagattt gatgcccggg tcgggtcctg cttgagccga tgcgcgtctg
      961 acccctggat gcaatgcatc cgcctcgggt attccgcctt cgtgccgctg aagaagcgcg
     1021 cagttgagcg ggtgctggtt gaggtcaggt ctgtgacttc gagcgcatgc aggttttcgt
     1081 cctgcgtgta ggcgatcttg gtttggccgc cgaagcggat gttccgcatc gcgacgccgt
     1141 gggtcgagtt gacgtgagcg ccgacgacca gggcgccgtg ctcggcgagc acctcgtatg
     1201 ccttgagcac gtcggatgtc gcgccaacct caccactgcc gaagcgatcc tcgggaatcc
     1261 caagtgaaag aagcaggtgc tccatcaacc gggttgatgt cgatgggtcg aagatggcca
     1321 ggatgtggaa cccgaacgtt gcggtgaact cgaagcccgg cagcaccagg accttgctca
     1381 tgaggtcgcg gtattccgca agcagggcag attcgctatc ggtaattcgg ccgagtcgtt
     1441 cgaggtaggt gaggtcctcg atctcgtgtt tcagcctggt ccagccggcg accgagttgt
     1501 gatcggtcag agcgatgatg tcgaggctct gctcggccgc tgtgcggagg aggtcgatag
     1561 gtcggatgcc ggaatcgtga taatcgattg atccgggagt gtggatgtgc aggtccgcgc
     1621 ggtaccactg atcggtttgc ttcggggccg gcttgtggga atccggggca ccctgcgacg
     1681 ccgttcgcgc acgcgcgcgc cgtcttggct cgctgatgga aatctccttc cgttgtggga
     1741 gcgcgctcaa aatcaaaaat cgggccgaat cggcaaatag ggcacgctca ctgtgctgtt
     1801 tcgatgctac cacacccgtc ccgggcgttc gaaacgcaga ctggatgacg catactaccg
     1861 ccgacatgat gagctcgatc ggcacacccc atgttattga tatcgcgatc gtcggcgcgg
     1921 tcgtcctgtt cggcgcggct ggattttggc gcgggctgaa caaggagctg tttatcacgg
     1981 cgtcattgct actcggttac gtcgtgacgc tcgagtgggc gccacgctgg ggacgctggc
     2041 tcggtgatcg gttttcggcg ctctcaactg ccgacggaca gtacgttgcg attgccggca
     2101 cacttctcct gtcgacgctg attctcggct atatcagctg cacggtcgcc gcgcttccgc
     2161 ctgccgatct gccgggccga tttggcggcc tggtactcag cgcggccaat gccacctttg
     2221 cggtcaccgt cctgattgcc cgggcgcgcc aacttgttct cgattcgagc caacagcgga
     2281 cgttgcggga aacccggatc ggtgattggc tctcccgcaa cctggactgg gtggttctgg
     2341 cgctggtcgt ggtcggcttc ctgctgctcg ccggcggcgc catgacacga cggcgccgtg
     2401 cggcattgat c
//
LOCUS       DASSGX010000444         3222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_950878, whole genome shotgun sequence.
ACCESSION   DASSGX010000444 DASSGX010000000
VERSION     DASSGX010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950878"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="VFI12_07930"
     CDS             <1..902
                     /locus_tag="VFI12_07930"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HET7056377.1"
                     /translation="NFGVAGFSDDGISTANSALMAEALLASKRIGKPIMVHCEDPFLI
                     GGVMHEGEVSARLGLPGIKSAAEESFIARDCLLARETGGWLHILHVSTAIGLQIVKYA
                     RQIGARVTAEVMPHHLVMTDEWVAGDRTLRNTSERGPDAPAAHPDTKVNPPLRTSEDT
                     RVLLAGLANGDFDILATDHAPHAAHEKRDVPITKAAFGMIGLELAVPTMLALVRAGQL
                     TLPQVIDRFSTQPARLWDLPAGRIEPGAPATFTVIDPQRRWTITEDQLKTKSKNTPLL
                     GMEMTGRAVLTLIEGEAVHDLCE"
     gene            886..2034
                     /gene="carA"
                     /locus_tag="VFI12_07935"
     CDS             886..2034
                     /gene="carA"
                     /locus_tag="VFI12_07935"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HET7056378.1"
                     /translation="MTSANETQSKRYRPAHDAALALADGRVFRGRGFGAHADAAGEVV
                     FTTTMTGYQEVCTDPSFKGQLVCMTYPLIGNYGVVADDDESRRPWVSALIVREHSDHP
                     SNWRSTGTIDTYLKDNGIPGISGIDTRALTRHIRSVGDTRGVLVRDAAGLSDDELVER
                     AKAAPLPGEQPVVEEVSDDRVVTEGSEDGPHIVVLDCGVKRNIIRALLKRGARVTSVP
                     YGTNFADIAALNPDGVIVSPGPGDPANLDDGLDVVRALLDQKLPYFGICLGHQLLARA
                     IGADTEKLKFGHRGGNHPVLDTRSDHVSITAQNHGYVVTTDSLPSDSGWRVALVNLND
                     GSVEGFAHESLPVLSVQFHPEASPGPLDNGYLFDEFLDMVNTRVAATR"
     gene            2047..>3222
                     /gene="carB"
                     /locus_tag="VFI12_07940"
     CDS             2047..>3222
                     /gene="carB"
                     /locus_tag="VFI12_07940"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HET7056379.1"
                     /translation="MSGAIKSVLVLGSGPIVIGQAAEFDYAGTQACTALREEGIRTIL
                     INSNPATIMTDEDVADAVYIEPLTPEVVRRVIQRERPDGLLPSLGGQTGLNLAVQVAE
                     QGILERYGVKLLGTQLESIKAAEDRKLFKELLESIDEPTIQGQIVTSIPEAREFAKTV
                     SYPLVIRPAFTLGGTGGGIANNAHELDAIVTSGLGASPIHQVLVERSLQGWKEIEYEV
                     MRDGNDTCITICNMENIDPMGVHTGDSIVVAPSQTLSDKEYQMLRTSAIKIIRALKIE
                     GGCNIQYTLNPDSFEYAVIEVNPRVSRSSALASKATGYPIARMAAKIAIGKRLDEMVN
                     KVTGKTTAAFEPALDYCVVKIPRWPFDKFTKADRTIGTQMKATGEVMAIDRSFEGALM
                     "
BASE COUNT          616 a         1059 c         1014 g          533 t
ORIGIN      
        1 cgaatttcgg tgtggccggc ttttcggacg acggcatctc gaccgccaat tccgcgctca
       61 tggccgaagc gctgctcgcc agcaagcgga ttggcaaacc gatcatggtc cactgcgagg
      121 acccgttcct gatcggcggc gtgatgcacg agggtgaggt cagcgcgcgc ctcggactgc
      181 cggggatcaa gtcggcagcc gaagagtcgt tcatcgcccg cgattgcctg ctggcgcgcg
      241 aaaccggcgg ctggctgcac atcctgcacg tcagtacggc gatcggcctg cagatcgtca
      301 agtacgcccg gcaaatcggc gcgcgcgtga ccgccgaggt catgccgcat caccttgtca
      361 tgaccgacga gtgggtcgcc ggcgatcgca cactccggaa cacgagtgag cgcggccccg
      421 acgcgccggc agctcacccg gatacgaagg tcaacccgcc gcttcgaacg agcgaggaca
      481 cccgggtcct gctcgccggt ctggcaaatg gcgacttcga catcctcgcg accgatcatg
      541 ccccgcacgc ggcgcatgag aagcgggacg tgccgatcac gaaggccgcg ttcggtatga
      601 tcggcctcga gctggcagtg ccgacgatgc tcgcgctggt ccgcgccggc caattgacac
      661 tgccacaggt gatcgaccgg ttctcgacac agccggccag actctgggac ctgccggccg
      721 gacgaattga gcccggcgcg ccggccacct tcacggtcat cgatccacag cgtaggtgga
      781 cgatcaccga ggaccaactg aagaccaaat cgaagaacac gcccttgctc ggcatggaga
      841 tgaccggccg ggcagtgctc acgctcattg aaggcgaggc agtccatgac ctctgcgaat
      901 gaaacccagt cgaaacggta ccggccggcc cacgacgcgg ccctcgcgct ggcggatggc
      961 cgcgtgttcc gtggtcgcgg attcggcgcc cacgcggacg ctgccgggga agtcgtcttc
     1021 acgacgacca tgaccggtta tcaggaggtc tgcaccgatc cctcattcaa ggggcagctc
     1081 gtctgcatga cctatccgct gatcggcaat tacggtgttg tggcggacga cgacgaatcc
     1141 cgccggccgt gggtgtcggc gctgatagtc cgcgagcact cggatcatcc gagcaactgg
     1201 cggtcgactg gcacgatcga cacatacctc aaggacaacg gcattcccgg tatctccggc
     1261 atcgacaccc gggcattaac tcgccatatc cgctcggtcg gcgacacgcg cggtgttctc
     1321 gtccgggacg cggccggcct ctcagacgac gagttggtgg agcgcgccaa ggcggccccg
     1381 ttacccggcg aacaaccggt cgtcgaagaa gtctcggacg accgcgtcgt caccgagggc
     1441 tccgaggatg gaccgcacat cgttgtcctc gattgcggcg tcaagcggaa catcattcgt
     1501 gcgctcctga agcgtggagc gcgggtgacc agcgtgccgt atgggacaaa cttcgcggac
     1561 atcgccgcgc tcaacccgga cggcgtgatc gtttcgcccg gacccggcga ccctgcaaac
     1621 ctcgacgacg gcctcgacgt tgtgcgcgca ctgctcgatc agaagctccc ttacttcggg
     1681 atctgcctcg gccaccaatt gctggcccgg gcgatcggcg cggacaccga gaagctgaag
     1741 ttcggccatc gtggcgggaa ccacccggtc ctggacacgc ggagcgacca cgtttcgatt
     1801 acggctcaga accacggcta cgtcgtcacg accgattccc tgccgagcga ttccggctgg
     1861 cgggtcgccc tggtcaatct gaacgacggc tcggtcgaag gcttcgcgca cgagtcgctg
     1921 ccggtgctgt cggttcaatt ccaccccgag gcgagtcccg gaccgctgga taacggctat
     1981 ttatttgatg aattcctcga tatggtgaac acgcgagtcg cagctactcg ctaagggaga
     2041 tcccgcgtgt ctggcgcaat caagagcgta ctggtgctcg gctctggccc gatcgtgatc
     2101 gggcaggccg ccgaattcga ctatgcgggc acgcaggcct gcactgccct gcgcgaggag
     2161 ggcatccgca cgatcctgat caactccaat ccggccacga tcatgaccga cgaggacgtg
     2221 gccgacgcgg tctacatcga gccgctcacg ccggaggtgg tccgccgcgt gatccagcgc
     2281 gagcgcccgg acggcctgct gccctcactc ggcggccaga ctggcctcaa cctcgcggtt
     2341 caggtcgcgg agcagggcat tctcgagcgg tacggtgtca agctcctcgg gacgcagctt
     2401 gagtcgatca aggcggccga agatcggaag cttttcaagg agctgctcga atcgatcgac
     2461 gagccgacga tccagggtca gatcgtgacc tcaatccctg aggcacggga gttcgccaag
     2521 acggtctcct acccgctcgt catccggccg gccttcacgc tcggcggcac cggcggtggc
     2581 attgccaaca acgcccacga gctcgacgcg atcgtgacca gcggtctcgg cgccagcccg
     2641 atccaccagg tcctggtcga gcgctcgctc cagggttgga aagagatcga gtacgaagtg
     2701 atgcgggatg gcaatgacac ctgcatcacc atctgcaaca tggaaaacat tgacccgatg
     2761 ggcgtccaca ccggagacag catcgtggtc gcgccgtcgc agacgctgtc ggacaaggag
     2821 taccagatgc tccgcacgtc cgcgatcaag atcatccgcg ccctcaagat cgagggtggc
     2881 tgcaacatcc agtacacgct caacccggac tcgttcgagt acgccgtgat tgaggtcaat
     2941 ccgcgcgtca gccgctcatc ggctctggca tcgaaagcca ccggctatcc aattgcgcga
     3001 atggccgcca agatcgccat cggcaagcgg ctcgatgaga tggtcaacaa ggtcaccggc
     3061 aagacgaccg ctgcctttga acctgcgctg gactactgcg tggtcaagat cccgcgctgg
     3121 ccgttcgata agttcaccaa ggcagatcgc acgatcggca cgcagatgaa agcgaccggc
     3181 gaggtgatgg cgatcgatcg ctccttcgaa ggcgcgttga tg
//
LOCUS       DASSGX010000445         2373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_513540, whole genome shotgun sequence.
ACCESSION   DASSGX010000445 DASSGX010000000
VERSION     DASSGX010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513540"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..1472)
                     /locus_tag="VFI12_07945"
     CDS             complement(159..1472)
                     /locus_tag="VFI12_07945"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HET7056380.1"
                     /translation="MLARLIAFWAYLKESLWALPLSLVALAVALAIAAVEIHIVEATS
                     AWWIYGGTGENAADFLSSLLSSMINMATLAISITMVVLTLAAQQLGPRLIFNFMGDHR
                     TQLALGTFIGTAVYLLLVLRIVQGRGLESVPNLAVTIGTVLVLVSVVTLVLFVHHLAR
                     SIVADTMIDRVGTALDHAIGRYLPERREQDPSAGVPIARHTGAPLRSDEGGYVQVINY
                     DKLVAAAVEADAAIELDVRPGHHLLEQGTYGWAAPPSALTDDLRSEVENSVLIGKERT
                     VVQDVEYSVRQLVEIALRALSSGVNDPYTALAAIDRLALSLGRVLEHGPPRVVWRDEE
                     GKIRLVAPGSDFEGILDVAFTQIRQAASDHAAILIRLAEKLGQLANQADPHQCATLSK
                     HLELVMAAGRRNLPEKADQVVLEKRVEFALAACRKADRPAEAGVR"
     gene            1635..2081
                     /locus_tag="VFI12_07950"
     CDS             1635..2081
                     /locus_tag="VFI12_07950"
                     /inference="COORDINATES: protein motif:HMM:NF021098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HET7056381.1"
                     /translation="MEHHERHMTETEGNDRRRETVATVPLVAETAAISKRFRTTGKVR
                     VKVRTETDRRTLTEELAGEDIEIERVPRNELVEAVPEVRTENDVTIIPLVEEQLVVEK
                     RLVLVEEVRLHRVHTKQTVTVPVELRAERADVEHVEAQSQERPSRS"
     gene            2084..>2373
                     /locus_tag="VFI12_07955"
     CDS             2084..>2373
                     /locus_tag="VFI12_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HET7056382.1"
                     /translation="MDRTLSALYDQRNDAMSAVDDLAALGIPRDNITMVARSEPGEAA
                     GGGAYGAEREQPGFWDAISNLFVSDEDKRIYEEGISQGGATIIARVDESQMDR"
BASE COUNT          452 a          754 c          808 g          359 t
ORIGIN      
        1 gtgatctacc ggcccgacgc ggagctgatc tctcattact attccgcgga tctgtcgcgg
       61 cagttcgatg cctggatctg gttcgacgag acgggcccgg ttacggcggt gccggcccct
      121 ggtgtcgccg aggagcggga gacctatccg ttcggcctct agcggacgcc tgcctccgcc
      181 gggcggtccg ccttgcggca ggcagcaagt gcgaactcga cccgtttctc caacacgacc
      241 tgatcggctt tttcgggcag gttccgacgg ccggccgcca tgacgagctc caggtgcttg
      301 gagagcgtgg cgcactggtg gggatcggcc tggttggcca gctgccccag cttctcggcc
      361 agccggatca ggatggcagc gtggtcgctc gccgcctggc gaatctgcgt gaaggcgacg
      421 tcgagaatgc cttcgaaatc cgaccctggc gcaacgagcc ggatctttcc ctcctcatca
      481 cgccagacca cacgcggggg cccatgctcc agcacccgtc cgagcgatag agccagccgg
      541 tcgatggcag caagagcggt gtagggatca ttgacgccgc tcgagagggc cctgagcgcg
      601 atctcgacaa gctgccggac cgagtactcg acgtcctgca ccaccgtgcg ctccttgccg
      661 atcagcacgc tgttctcgac ctcgctacgc aggtcgtctg ttagcgccga gggtggtgcg
      721 gcccagccgt aggtgccctg ctcgagcagg tggtgaccgg gccgcacgtc gagctcgatg
      781 gccgcgtcgg cctcgactgc ggccgccacc agcttgtcgt agttgatgac ctgcacgtag
      841 cccccctcgt ccgagcgcag cggagcgccc gtgtgccgcg caataggcac gccggccgac
      901 gggtcctgct cgcgccgctc cggcaagtac cggccgatgg cgtggtcgag tgcggttccg
      961 acgcgatcga tcatggtgtc cgctacgatg gatcgcgcca ggtggtgaac gaacagcacc
     1021 agagtgacga cgctgacgag caccaggacc gttccgatgg tcacggcgag gttgggaaca
     1081 ctttccagtc cgcgcccctg cacgatccgc aatacgagca gcagatagac cgccgttccg
     1141 atgaacgtcc cgagcgcgag ctgcgtacgg tggtcgccca tgaagttgaa gatgagccgc
     1201 ggcccgagct gctgcgcggc aagcgtgagc acgaccatcg tgatggagat ggcgagggtg
     1261 gccatgttga tcatggagct gagcaggctc gacaggaagt ccgcggcgtt ctcgcccgtg
     1321 ccgccataga tccaccaggc ggaggtcgcc tcgacgatgt ggatctcgac cgccgcaatc
     1381 gccagcgcca cggccagagc gacgagcgac aggggcaggg cccagaggct ttccttgagg
     1441 taggcccaga acgcgatcag gcgagcgagc atcgagcacc ccttcaggaa gacgcgttga
     1501 cggcctcggc cggtggcggc atgcgagcgc tggccgagtc cgccccaaca cgtcagcgcc
     1561 tgccaaaatc ccatagaaaa gtgaaggtta gcgcaaaatg gcgcaggaac atctgaagta
     1621 cggcgtcgtt aaccatcgaa caccacgaga ggcacatgac ggaaaccgaa ggtaacgaca
     1681 gacgccggga gacggttgca accgtgccgc tggtcgccga aaccgccgcg atatcgaagc
     1741 ggttccggac caccggaaag gtgcgggtga aggtgcgcac cgagaccgac cggcgcacgc
     1801 tcaccgagga gctcgcgggc gaggacatcg aaatcgagcg ggtgccccgc aacgagctgg
     1861 tcgaggccgt accagaggtt cggaccgaga acgacgtcac gatcatccct ctcgtggagg
     1921 agcagctcgt cgtcgagaag cggctggtgc tcgtggagga ggtgcgcctc caccgggttc
     1981 acacgaagca gaccgtcacc gtgcccgtag agctgcgggc cgagcgggcg gacgtcgagc
     2041 acgtcgaggc gcaatcacag gaacggccga gcaggagctg atcatggacc ggacattatc
     2101 cgcactttac gaccaacgga acgacgcgat gtcggccgtg gacgacctcg cggcattagg
     2161 cattcctcga gacaacatca cgatggtggc ccgctccgag ccgggtgagg cggctggcgg
     2221 cggagcgtac ggcgccgagc gcgagcagcc cggcttctgg gacgcgatct ccaacctgtt
     2281 cgtctcggac gaggacaagc ggatctatga ggaggggatc agccaaggcg gcgcaacgat
     2341 catcgccaga gtcgacgaga gccagatgga tcg
//
LOCUS       DASSGX010000446         2514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1167533, whole genome shotgun sequence.
ACCESSION   DASSGX010000446 DASSGX010000000
VERSION     DASSGX010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1167533"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VFI12_07960"
     CDS             complement(<1..576)
                     /locus_tag="VFI12_07960"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET7056383.1"
                     /translation="MASTNGKGANGNGAFDVRHMSRNLLDGVERAGARAMLKGIGLTD
                     DDLKKPMILIANTWIETMPCNLGLRTLAHAVKEGVRAAGGVPMEMNTIAISDGVTMGT
                     EGMKTSLISRELIADTVELVGRGHMFDAVIGLGACDKTLPGLSLALARLNIPGFLLYG
                     GTIMPGEVNGQEATVRSVYEKIGAYKAGKITE"
     gene            684..1535
                     /locus_tag="VFI12_07965"
     CDS             684..1535
                     /locus_tag="VFI12_07965"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET7056384.1"
                     /translation="MAGLTFGIQTPPFHDRARMVADWQRFESLGWDSLWLPDHLVPTF
                     RRDLPFFEAWTLLAALAMVTNRVRIGVLVSCNTFRHPSLLAKQAATVDHVSNGRLEVG
                     LGTGWVEFEHTMFGIEYPDNAVRVDRFGEAVQVLDLLLRNEISSFQGTYYQLDEASFR
                     PAPIQQPRPPFTLAAHGPKMLRLIAPYVDRWNSMGSPAEMGERGARLDEALISAGRDP
                     GEVIKSVLYVPSVTPDEDPWASADAFVDFVGRYREQGVRDFIFQPAHAPAELIERVSH
                     DLLPSLR"
     gene            1594..1854
                     /locus_tag="VFI12_07970"
     CDS             1594..1854
                     /locus_tag="VFI12_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HET7056385.1"
                     /translation="MSDITGILNLLFTVLSLAVIARALLSWFDPGMRYPVSQLLVDLT
                     EPIIAPIRRIVPPVGGMIDLSPLIALILLQVIERVIIRSLNS"
     gene            1951..2073
                     /locus_tag="VFI12_07975"
     CDS             1951..2073
                     /locus_tag="VFI12_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056386.1"
                     /translation="MGGVVRIVSIVGFALLALLIILMIISFTGGFEHTLSNPPS"
     gene            complement(2136..>2514)
                     /locus_tag="VFI12_07980"
     CDS             complement(2136..>2514)
                     /locus_tag="VFI12_07980"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056387.1"
                     /translation="GVSLYMAELLRLKQIVDAANASPILYLLDEILHGTNTTERRIAA
                     RSVIHQLVRTGSIGAVSTHDLELIDTGLAHAAVCVHLIDQVVDGPNGPEMFFDYTLRP
                     GLAPTSNALRLLELVGLGDGGQT"
BASE COUNT          399 a          808 c          806 g          501 t
ORIGIN      
        1 ctcggtgatc ttgccggcct tgtaggcgcc gatcttctca tagaccgagc ggaccgtcgc
       61 ttcctgcccg ttcacctcgc ccggcatgat cgtgccgcca tagagcagga agccggggat
      121 gttgaggcgg gccagcgcca gagagaggcc aggcagcgtc ttgtcgcacg cgccgagacc
      181 gatcactgcg tcgaacatgt gcccgcgtcc aaccagctcg accgtgtcag cgatcagctc
      241 gcgggagatg agcgaggtct tcatgccctc cgtgcccatc gtcacgccgt ccgaaatggc
      301 gatcgtattc atctccatcg gcacgccgcc ggcagcgcga acgccctcct tgaccgcgtg
      361 ggcgagcgtg cgcaggccga gattgcacgg catcgtctcg atccacgtgt tcgcgatcag
      421 gatcatcggc ttcttgagat cgtcgtccgt caggccgatg cccttgagca tcgcccgggc
      481 gcctgcccgc tcgacgccgt cgagcaggtt ccggctcatg tgccggacat cgaatgcgcc
      541 gttgccgttt gcgcccttgc cgtttgtgct tgccatggcg gtgcctcgtg tctcccctga
      601 tttcgcgcac aatgccggtg cgcgctggaa tgcgttggcg ctagtttcgc atagcaggca
      661 gagtgacagg acggtattcg gggatggcag gacttacctt cggcattcag acaccgccgt
      721 tccacgaccg ggcacgcatg gtggccgact ggcaacggtt cgaatcgctc ggctgggact
      781 cgctctggct gccggatcac ctcgtgccga cctttcgccg cgacctgccc ttcttcgagg
      841 catggacgct gctcgccgcc ctggcgatgg tcacgaaccg ggtccggatc ggcgtgctgg
      901 tcagttgcaa caccttccgc cacccctcgc tgctcgccaa acaggcggcg acggtcgacc
      961 atgtcagcaa cggtcggctt gaggtcgggc tgggaaccgg gtgggtcgag ttcgagcaca
     1021 cgatgttcgg aatcgagtat ccggacaacg ccgtgcgggt cgaccggttt ggcgaggcgg
     1081 tgcaggtact cgatctcctg ctccggaacg agatcagcag cttccagggc acgtactatc
     1141 agcttgatga ggcgtcattc cggccggcgc cgatccagca gccgcgccct ccgtttaccc
     1201 tcgcagcgca tgggccgaaa atgctccgcc tgatcgcgcc gtatgtcgat cgctggaact
     1261 cgatgggctc gccggccgag atgggcgagc gaggcgcgcg actcgatgaa gcgctgatct
     1321 cggccgggcg cgatcccggc gaggtcatca agtcagtcct ttacgtgccg agcgtgacgc
     1381 cggacgagga cccgtgggcc tcggccgatg cctttgtcga tttcgtcggc cgctaccggg
     1441 agcagggcgt gcgggacttc atcttccagc cggcgcatgc accggctgag ctgatcgagc
     1501 gcgtctcgca cgatctgctg ccaagcctgc ggtgaggcat gactatactg cgcaccatca
     1561 atcagacatc agccacgctt cggagtacgc cacttgtctg acatcaccgg cattctgaat
     1621 ctgctcttca ccgtgctctc gttagcggtg attgcgcgcg cgctgctctc ctggtttgat
     1681 ccgggcatgc gctatccggt cagccaactt ctcgttgatc tgaccgagcc gatcatcgcc
     1741 ccgatccgcc gcatcgtgcc gccggttggc gggatgatcg atctctcgcc gctgattgcg
     1801 ctcatcctgc tccaggtgat cgagcgcgtg atcatcagat cgttgaattc gtagcgcata
     1861 tgccgggacg ggacggccgg ttgtcccgta gaatggtcga gtacgggctt gccggcgttt
     1921 ggtcggaatc agtcagatgg agcggcagca atgggcggag ttgttcgaat tgtgagcatt
     1981 gtggggttcg ccctgctcgc gctcctgatt atcctgatga tcatctcgtt caccggcggc
     2041 ttcgagcaca cgctgtccaa tccgccgtcc taggctgaca taccttctcc acatttgaag
     2101 gagttcgatg taacgcgggc gcactggcgc ccgcgttagg tttgcccccc atcccccagc
     2161 ccgaccaact cgagcaggcg gagcgcgttc gacgtgggcg cgaggccggg tcgcagcgtg
     2221 tagtcgaaga acatctccgg tccgttcggt ccgtccacaa cctggtcgat caggtggacg
     2281 caaaccgctg cgtgggcgag gccggtgtcg atcagctcca ggtcgtgcgt cgaaaccgcg
     2341 ccgatcgagc cggtccggac gagctggtgg atgaccgacc gggcggcgat ccggcgctcg
     2401 gtcgtgttcg taccgtgcag gatctcgtcg agcaggtaga ggatcgggct ggcgttcgcc
     2461 gcgtcgacga tctgcttcaa gcggagcagc tcggccatat agagcgagac cccc
//
LOCUS       DASSGX010000447         3125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_622109, whole genome shotgun sequence.
ACCESSION   DASSGX010000447 DASSGX010000000
VERSION     DASSGX010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622109"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2291)
                     /locus_tag="VFI12_07985"
     CDS             complement(<1..2291)
                     /locus_tag="VFI12_07985"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HET7056388.1"
                     /translation="MASRRALIGFLGLLLGLIIAGGRPMSTAAHATLGSSDPPANEIL
                     SSSPGRIELRFTEPVEETYTKIILVDETGAEVAGTSTSLDSSDATIARMVIPDNLARG
                     TYSVVWRTLSAADGHRFSGYFAFTIGSSSDVRTVIPPAFTDSGGAPFWFEVLSRWFAF
                     LTLSLLTGLWLTWLLVIRPGLAPVWQIGPAVVSRVRGYALVVGLLYLVGSVIALLVQA
                     SGQSDGHVLGAIRDTLVDTRWGRIWTLRLIFGAITTIVFLAAAWWWPRRRLLTYAGLL
                     ALSALLPLPHALISHASAQTAGRNEAIFADYAHLLAMGLWIGGLGAIAATLTASGDLA
                     PGGRRAFLVRIMPRFSAIALTCWAALGLTGLYSGYLQIGSWDGLFDTPYGRALVYKLA
                     ILAAVLVVASVNLLVVTRKIRTATSEAAPRWWRRFAVLIALELIGVAAVLIFVGRMTS
                     LEPSRPILAERENQQTTAFTIGERSATLSMAPGSAGPNHFRLDVHGDPLASNVKASIL
                     LVPPLDMAGQKAVTLERTTGNSFEAHTAEMSVVGDWKMTVTLSQVGAFQWSADLTHAA
                     AERKPGVRSINLPAWTVTSTGLIGFLAVMAGVVGLVVGWRGGNRSTRREGFGLGTVAI
                     VAGLLLITQGRADVASAGIPLDTPNPVPLTDQAIVAGRTAFQANCVACHGPGAAGDGP
                     AAQGMVPPPANLLEGHALYHADAEFFNWIRNGKPGTAMPGFSDTLTDEEIWSTIHYIR
                     ELQAQHAEAVGTPTPVAAPTKGAT"
     gene            complement(2301..2948)
                     /locus_tag="VFI12_07990"
     CDS             complement(2301..2948)
                     /locus_tag="VFI12_07990"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HET7056389.1"
                     /translation="MPSPFVFTFRSLPALVLLLIAVIAQPRLTSAHADLESANPPAGG
                     TVAALPPKMTLIFAEEVKPGSAVVEVTGPNGARADAGDADVDLTDSARKTVTVSLFAG
                     GPGAYSVHWETVSNTDNHPLSGDYTFTVTGSAAGATPAATPTEANAADLTPTAAPTED
                     PANGNPLNAKGDFDSRALGISVGVGLIGVAAIFGFWFVVRPKNPRFGSRSGRDQR"
BASE COUNT          542 a         1050 c         1071 g          462 t
ORIGIN      
        1 gtcgctcctt tggtcggcgc cgcgaccggc gtcggcgtgc ccacggcttc ggcgtgctgc
       61 gcttgcagct cccggatgta atgaattgtg ctccagatct cctcgtcggt gagcgtgtcg
      121 gagaatccgg gcatggccgt acccggcttg ccgttgcgaa tccagttgaa gaactcggca
      181 tcggcgtgat agagcgcgtg cccttcgagc agattggccg gcggagggac catgccctgc
      241 gcggccggtc cgtcacccgc cgcgcccgga ccgtgacagg cgacgcagtt ggcctggaag
      301 gcggtccgtc cggcgacgat cgcctggtcg gtcagcggga cggggttcgg cgtatcgagc
      361 gggatgcccg cggaggcgac gtcagcccgg ccctgcgtga tcaggagaag gccggccacg
      421 atcgcgaccg tgccgagccc gaatccctcg cggcgggtgg agcggttgcc gcctcgccac
      481 ccaactacga gcccgaccac cccggccatg accgccagga agccgatcag cccggtgctc
      541 gtgaccgtcc acgctggcag gttgatcgag cggacgcccg gctttcgctc cgccgccgcg
      601 tgcgtgagat cggccgacca ctggaaggcg cccacctggc tgagggtgac ggtcatcttc
      661 cagtcgccga ccacgctcat ctcggccgtg tgcgcttcaa agctgttgcc ggtggtgcgc
      721 tccagcgtga ccgctttctg cccggccata tcgagcggcg gtacgagcaa gatgctcgcc
      781 ttgacgttgc tggccagcgg atcgccgtgc acatcgagcc ggaagtggtt cggaccggcc
      841 gacccgggcg ccatcgagag tgttgccgat cgctcgccga tggtgaacgc ggtggtctgc
      901 tggttctctc gctcggccag gattgggcgg gagggctcca ggctggtcat ccggccgacg
      961 aagatcagaa cggccgccac gccgatcagc tccagcgcga tcagcaccgc aaatcgtcgc
     1021 caccaccggg gcgcggcctc ggatgtggcg gtacggatct tgcgggtgac gaccagcagg
     1081 ttgacggagg cgactaccag caccgccgcc aaaatagcca gcttgtagac aagcgcgcgg
     1141 ccatacggcg tgtcgaacag cccgtcccag gatccgatct ggaggtagcc gctgtacagc
     1201 ccagtcagcc cgagggccgc ccagcacgtc agggcgatcg ccgagaagcg gggcatgatc
     1261 cggaccagga acgcccggcg cccgccgggg gcgagatcgc cggacgcggt caaggtcgcg
     1321 gcgatcgccc cgagtccgcc gatccagagc cccatcgcca gtagatgcgc gtagtcggcg
     1381 aagatcgcct cattccggcc ggccgtctgc gccgatgcgt gcgagatcag cgcgtgcggc
     1441 agcggcagca gcgcgctgag cgcgagtagc ccggcgtagg tcagcagccg gcggcgcggc
     1501 caccaccagg cggccgccag gaagacgatc gtggtgatcg ccccgaaaat gagtcggagc
     1561 gtccagatcc ggccccagcg ggtgtcaacc agcgtgtccc ggatcgcccc aagcacgtgg
     1621 ccgtcggact gaccgctggc ctgtacgagc agcgcgatga ccgagcccac cagataaagc
     1681 agcccgacga ccagtgcgta gccccgtacc cgggagacga ccgccggccc gatctgccag
     1741 accggcgcca gccccggcct gatcacgagc aaccaggtca gccagagccc ggtcaacagg
     1801 ctcagcgtca ggaacgcgaa ccagcgtgac agcacctcga accagaaggg agcgccaccc
     1861 gaatcggtga atgccggcgg gatgacggtc cgcacgtcgg aggacgagcc gatggtgaag
     1921 gcgaaatacc cggaaaagcg atgcccgtcg gcggcggaga gggtacgcca gacgaccgaa
     1981 taggtgcccc gggcgagatt gtccgggatg accattcgcg cgatcgtggc atccgacgag
     2041 tcgagcgacg tcgacgtgcc agccacctct gcaccggttt cgtcgaccag gatgatcttc
     2101 gtgtaggtct cttctaccgg ctcggtgaag cggagctcaa tccgccccgg cgaggaggaa
     2161 aggatctcgt tcgcgggcgg atcggagctg ccgagcgtgg cgtgcgccgc cgtcgacatt
     2221 ggccggccac cggcgatgat caggccgagg agtagtccca ggaacccgat tagcgcgcga
     2281 cgactggcca tcttcctgcg ctacctctga tcccgcccgg aacgcgatcc aaagcgcgga
     2341 ttcttcggcc gcacaacgaa ccagaacccg aagatcgccg ccaccccgat caaacccaca
     2401 ccgacgctaa ttcccagcgc gcggctgtcg aaatcgccct tggcgttgag cgggttgccg
     2461 ttggccggat cttcggtcgg cgcggcagtg ggcgtcagat cggccgcatt cgcctcggtc
     2521 ggtgttgcgg cgggcgttgc tcctgccgcg ctgccggtca ccgtgaaggt gtaatcgccg
     2581 gagagtgggt ggttgtccgt gttcgagacc gtctcccagt ggaccgagta ggctcccggt
     2641 ccgcccgcaa agagcgagac ggtcaccgtc ttccgcgcgg agtcggtcag gtcgacgtcc
     2701 gcgtctccgg catcggcccg cgcgccgttc ggcccggtca cctcgacgac cgcgctcccc
     2761 ggcttgacct cctccgcgaa gatcagcgtc atcttcggcg gcagtgcggc gacggtcccg
     2821 ccggcgggcg ggttcgccga ctcgagatca gcatgcgcgc tcgtgaggcg gggctgcgcg
     2881 atgacggcaa tcaacaggag gaccagcgcc gggagcgaac gaaacgtgaa tacgaacggg
     2941 gagggcacga ggctcagcat ccttgcctaa ccgaaaaatc attcaataga tgatacctac
     3001 ctggcgccat tccggattgc gcgcatccgg cactcatgca agcgaaaccc ccgcccgtca
     3061 agtgacgggc gggggtttcg cttgcatgag tgccggatgc gcgcaatccg gaatggcgcc
     3121 aggta
//
LOCUS       DASSGX010000448         2698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_78469, whole genome shotgun sequence.
ACCESSION   DASSGX010000448 DASSGX010000000
VERSION     DASSGX010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78469"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="VFI12_07995"
     CDS             complement(<1..474)
                     /locus_tag="VFI12_07995"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HET7056390.1"
                     /translation="MTGQQFILAIDQGTTSSRALLFDHDGAMVALAQRPIDVTFPHPG
                     WVNQDARQLWSVTEAVSHEAMAQASVKFGQIAAIGITNQRETTILWERKTGEPLAPAV
                     VWQSRQSADVIRAMERRGVGEKYRSITGLVLDPYFSASKIRWLFDQEPYLEARARR"
     gene            537..2186
                     /gene="aroB"
                     /locus_tag="VFI12_08000"
     CDS             537..2186
                     /gene="aroB"
                     /locus_tag="VFI12_08000"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HET7056391.1"
                     /translation="MEIERIVLIGFSGTGKSTIARLLANALDWTAIDTDRAVEIVATK
                     TIPDIFAQDGEAIFRDLERTLLLHSLDQTQVVIATGGGAIISEDVWTEVALRRPGTLT
                     IALDSDPATILNRLRNQQAIANETIERPLLATADPLQRITDLKASRQAAYDRADLTLV
                     TDTMSTADVVEQIASFVADSNEPDVTLDAPGGRSCIFVRPGIVDWTGTLVRGQWPKAR
                     QAFVVTDANVGPLHADCVRESLESAGLAVQIITVPSGEGSKSWMTAGDVIGTMLESGI
                     QRNDVVVALGGGVVGDLAGFASAVVLRGVGIVQIPTSLLAMVDSSVGGKTGVNHPAGK
                     NLIGAFYQPPLVIVDPELLRTLPSRELTQGWAEVVKHAVIQPSTPDQYRSDLFAFLER
                     NRDDLVALREPATSYAIRRNIVLKASVVEADEREASLRAILNYGHTIGHAIEAAEYRY
                     LHGEAISVGIHAANRIALLEGRIDDIREQSIAELLAGFSLPSRAGFDRATVRDRMRSD
                     KKRTAGRQTWVLPTGVAGVEQVTGISDEIIDAALDFVRSAD"
     gene            2363..>2698
                     /locus_tag="VFI12_08005"
     CDS             2363..>2698
                     /locus_tag="VFI12_08005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HET7056392.1"
                     /translation="MSDARISDLVGKLWSGEISRRDFMKRAAAAGLGAPVITAALSQG
                     AMASPGTGSSRALNRNQADASTLVIADSTIGNQWLTLDPAWFYEINSTAAMNLVNEAL
                     YHIPDGTKPT"
BASE COUNT          503 a          869 c          846 g          480 t
ORIGIN      
        1 tcgccgcgcc ctcgcttcta gataaggctc ctggtcgaac aaccagcgaa tcttgctcgc
       61 cgagaaatag ggatcaagga cgagaccggt gatcgaccgg tatttctcgc cgacgccgcg
      121 acgctccatc gcgcggatca catccgcact ctgccggctc tgccagacga ctgccggcgc
      181 gagtggttcg cccgtcttcc gctcccagag gatcgtcgtc tcgcgctggt tcgtgatgcc
      241 aattgccgcg atctgtccaa atttgaccga ggcctgcgcc attgcctcat gggagacagc
      301 ctcggtcacc gaccaaagct gtctggcgtc ctggttgacc cagccgggat gcgggaaggt
      361 gacatcgatc ggccgctgcg ccagtgcgac catcgcgccg tcatggtcga acaggagtgc
      421 ccgcgaagag gtcgtcccct ggtcgatcgc gaggatgaat tgctgtcccg tcattcccgt
      481 gcctccgctt agtatgctcc cacaggttga ccggaatcgc atgagtgggt tgagcagtgg
      541 aaatcgagcg catcgtcctg atcgggttca gcggcaccgg caagagcacc atcgcgcgac
      601 tgttggcgaa cgcgctggac tggaccgcga tcgacaccga ccgggcggtc gagatcgtcg
      661 cgacgaaaac gattccagat atcttcgcgc aggacggcga ggcgatcttc cgcgacctcg
      721 agcgaacgct cctgcttcat tcgctcgacc agacccaggt cgtgatcgcg accggcggcg
      781 gcgcgatcat cagcgaagac gtctggaccg aagtggcctt gcgccggccc ggcaccctga
      841 cgatcgccct cgattccgat ccagcaacga tcctgaaccg gctccggaat cagcaggcta
      901 ttgccaacga gacgattgaa cgaccactgc ttgccaccgc cgacccgctc cagcgcatca
      961 ccgacctgaa ggcgagccgg caggcggcat acgaccgggc cgatctgaca ctggtcactg
     1021 acacgatgtc gacggccgat gttgtcgagc agattgcgtc gttcgtcgcc gattcgaatg
     1081 agccagatgt cacgctggat gccccgggcg ggcgatcctg catcttcgtg cggccgggca
     1141 ttgtcgactg gacgggcacg ctggttcggg gccagtggcc gaaggcgcgc caggcattcg
     1201 tcgtcacgga tgcgaatgtc ggcccactgc acgccgattg cgtccgggaa tcgctcgagt
     1261 cggccggact tgccgttcaa atcatcaccg tgccgagcgg cgaaggcagc aagagttgga
     1321 tgactgccgg cgacgtgatt ggaacgatgc tggagagcgg cattcagcgg aacgatgtgg
     1381 tcgtggccct cggtggtggt gtcgtcggcg atttggcggg atttgcatca gcagtcgtct
     1441 tgcgtggagt cggcatcgtc cagatcccga cgagcctgct ggcgatggtc gatagctccg
     1501 tcggcggcaa gaccggcgtc aatcacccgg ccgggaagaa cctgatcggc gctttctacc
     1561 aaccgccgct cgtcatcgtc gatcccgagc tgctccgcac gctgccgtcg cgggagctca
     1621 cgcaaggctg ggccgaggtc gtcaagcatg cggtcatcca gccctcgaca cccgaccagt
     1681 atcgatcgga tctcttcgcc ttcctcgagc ggaaccgtga cgacctggtg gcactcaggg
     1741 agcctgcgac gagctacgcg atccggcgaa atatcgtgct taaggcgtcc gtcgtcgagg
     1801 cggacgaacg cgaagcgtcg ctgcgcgcga tcctcaatta cggccacacg atcgggcatg
     1861 ccatcgaagc cgctgaatac cgttacctgc atggtgaagc gatctcggtc ggcattcatg
     1921 cggccaaccg gatcgcgctg ctcgaaggtc ggatcgatga cattcgggag caatcgatcg
     1981 ctgaactcct cgccgggttt agcctccctt cgcgcgccgg gttcgaccgg gcgacggtgc
     2041 gtgatcggat gaggagcgac aagaaacgca ccgccgggcg ccagacctgg gtcctcccaa
     2101 cgggtgtggc gggagtcgag caggtcaccg gaatcagcga cgaaatcatc gatgcggcgc
     2161 tggatttcgt gcgcagcgct gattaatccg cggcccaaaa cacccccttg gcggattgcc
     2221 atcggtcgtg aatacttcgc gcagactcag gaaccggaac tcgtgctcgt gcccttcgcg
     2281 gcagcgctat ttcggcatcc ctgaacgtta tgtcgtgtcg gtgcgcgatg ccgcgctcac
     2341 caacagaccg tgaggagaag agatgtcaga cgcgcgcatt tccgatctcg tcggtaagct
     2401 ctggtccggg gaaatctccc ggcgtgactt catgaagcga gcggccgccg cggggttggg
     2461 cgccccggtg atcacagccg cactcagcca gggagcgatg gccagcccgg gcaccgggtc
     2521 cagccgagca ctcaaccgca atcaagccga tgcctcgacc ctggtaattg ccgactcaac
     2581 gatcggcaat cagtggctca ccctcgaccc ggcctggttc tatgagatca actcgaccgc
     2641 ggctatgaac ctcgtcaacg aggccctcta ccacatcccg gacggcacca agccgacc
//
LOCUS       DASSGX010000449         3710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_731270, whole genome shotgun sequence.
ACCESSION   DASSGX010000449 DASSGX010000000
VERSION     DASSGX010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731270"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..1290)
                     /locus_tag="VFI12_08010"
     CDS             complement(163..1290)
                     /locus_tag="VFI12_08010"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HET7056393.1"
                     /translation="MRRYHCAPPRLRASAPPRLRLLAFSTPIESTSSNARTSLKGSSS
                     MRALSRSDLRQAVPMHDAVELMKVAFRELSAGRAVSPVRAQVPIGDPNSMLLSMPGYV
                     ASEKAAGIKIVSFFGGNAGTAIPVIHALVYMIDADTGQPLGIMEGGYVTALRTGAVSG
                     AATDLLARPDSATLAVIGTGVQGVTQAAAVCCVRPITKIIGIDLRPDAQQRFVETVRR
                     DWPELAECEITTTANADEAVAVADVLCCSTTARQPVFSDAAVRPGTHINAVGAFTPDM
                     QEVPPATIARATVVVDNVDACLEEAGDLIKAMAEGAIGRAHWSRELGQVVSGEAPGRS
                     RDDEITFFKSVGNSVQDVVVGQRALARADEMGLGTELDLFA"
     gene            1523..2098
                     /locus_tag="VFI12_08015"
     CDS             1523..2098
                     /locus_tag="VFI12_08015"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HET7056394.1"
                     /translation="MTDPGLTRRLRQQGHRSGLAIGISMALAIAVLFGGFTWIYVQLD
                     PWARDFAGAEPAPTQTPRESASNSGGRATRTPTPEEDTSPEDQPAEEPAATETPKPES
                     TKIQAVNEDKGDFKADYQVVALEPVRLRSGPGVNFDIVVDGVPPGTQLQYLDDREPSQ
                     NPDADGDTEWLKFRLESGEEGWIRNIDVSGT"
     gene            2341..3420
                     /locus_tag="VFI12_08020"
     CDS             2341..3420
                     /locus_tag="VFI12_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HET7056395.1"
                     /translation="MQRVAIVGLGLIGGSIGLGLKRWAAEQAKGGQPPLQVIGFDTDL
                     DQQSYAKKINAVDKTEWRLPNAIAEADIVILATPVGAMQELLTDIAPHLTEGAIVTDV
                     ASTKADVVRWADETLPATVSFVGGHPMAGKALSIEGADADLFKGATWVVTPSVRASES
                     ALKNVLGLIAALGAEAFFVDAAEHDAYVAGVSHLPFVLAATLTNALGSDSSWKDMKSL
                     TAGGFKDMTRLAGGSPVMHRDIVLTNQDAILRWIDQFQAQLSAVRDLLARESIEGREH
                     EIEKFFERARDIRAEWSTQVTREGELLQQTGAELSMEGVGDQMSRMFFGSLFKRRMAE
                     ADRLGRSRNDRRKLQGKSDQSGDNS"
     gene            complement(3384..>3710)
                     /locus_tag="VFI12_08025"
     CDS             complement(3384..>3710)
                     /locus_tag="VFI12_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056396.1"
                     /translation="AMALRHGLARFDEFVVRDDASSIDEWLAQNDPVDGTPFRLIDDR
                     LREATLTPLPLGLIMVNLPDKRIVQIENRYGPLLRKDRGRMRREGEPVGQVYTYELSP
                     DWSLLP"
BASE COUNT          704 a         1343 c         1126 g          537 t
ORIGIN      
        1 gaccggtaca tgccaacggg ccagcgaggc ggcggcgggc agatgccatc tgcccctaca
       61 aggcggccgg atgggacgct tcgtccgaaa accgaaaacc gaaaaccgaa aactgaaaac
      121 cgaaacccaa aacccaaaac ccgaaaacct gaaacccaaa acctacgcaa acaggtccag
      181 ctccgtcccc aaccccatct catccgcccg cgccagcgcc cgctgtccca ccaccacatc
      241 ctgcaccgag ttgccgacgc tcttgaagaa ggtgatttcg tcgtccctcg accggcccgg
      301 agcctcgccg ctgacgacct gccccagctc gcgggaccag tgtgcccggc cgatcgcgcc
      361 ctctgccatc gccttgatca ggtcgccggc ctcctcgagg caggcatcga cgttgtcgac
      421 cacgaccgtc gcccgcgcga tcgtcgccgg cggcacttcc tgcatgtcgg gggtaaaggc
      481 gccgaccgcg ttgatgtgcg tccccggccg gaccgccgcg tcgctgaaga ccggctgccg
      541 cgccgtcgtc gagcagcaga gcacatctgc caccgcgacc gcctcgtccg cgttcgccgt
      601 cgtcgtgatc tcgcactcgg ccagttccgg ccaatcccgc cgcacggtct caacgaaccg
      661 ctgctgcgcg tcgggccgga ggtcgatgcc gatgatcttt gtgatcggcc gcacgcagca
      721 gaccgccgcc gcctgcgtca ccccctgcac gcccgtgccg atcacggcca gcgtcgcgga
      781 atccggacgc gcaagcaggt ctgtcgccgc gcccgagacc gcgccggtcc gcagggccgt
      841 cacatacccg ccctccatga tcccgagcgg ttgccctgtg tcggcatcga tcatgtagac
      901 cagcgcgtgg atgaccggga tcgccgtgcc cgcgttgccg ccgaagaagg agacgatctt
      961 gatccccgcc gccttctccg acgcgacata gccgggcatc gagagcagca tcgagttcgg
     1021 atcaccgatc ggcacttgcg cccgcaccgg cgagacagct cgccccgccg acagctcccg
     1081 gaaggccacc ttcatcagtt ccaccgcatc atgcatcggc accgcctgcc gcaaatcgct
     1141 ccgggacaac gcacgcatgc tggacgaccc tttcaatgac gttcgagcgt tagatgaggt
     1201 ggattctatc ggagtcgaga aggcgaggag gcgaaggcgc ggaggcgcgg aggcgcgaag
     1261 gcgcggaggc gcgcagtggt agcgtcgcat agcaatgcga cactcccgcc gcacccccag
     1321 cctcggagag gcttcctgcc taagcccggg gtttcaaccc cgggaaccac ggacgccaac
     1381 gcggaaaacg gaaaacggaa aacggaaaac ggaaaacgaa agaccaaaga cgaacgacca
     1441 actgctcgcc tccgcgcctc cgcgcctgat atacttccgc cgctccgcga tgcacgccgt
     1501 catcgccatt ccctgaggcc ccatgaccga tcccggactg acacgacgac tccgacagca
     1561 aggccatcgc tccggtctcg ctatcggcat ctccatggcc ctcgccatcg ccgtcctctt
     1621 cggcgggttt acctggatct atgtccagct cgacccctgg gcgcgcgact tcgccggcgc
     1681 cgaacccgcg ccgacccaaa caccgcgcga atcggcctcg aattctggcg gcagggccac
     1741 caggaccccc acgcccgagg aagacacctc gcccgaggat caaccggccg aagagccggc
     1801 cgcgaccgag acgcccaagc ccgaaagcac gaagattcag gccgtcaacg aggataaggg
     1861 cgacttcaag gccgattacc aggtcgtcgc cctcgaaccg gtccgccttc gcagcgggcc
     1921 gggcgtcaac ttcgacatcg tggtcgacgg cgtcccgccc ggaacccagc tccagtacct
     1981 ggacgaccgc gagccatcgc agaacccgga cgccgacggc gataccgaat ggctcaagtt
     2041 ccgcctcgaa agcggcgaag agggctggat ccgcaacatc gacgtcagcg gcacctgatc
     2101 ccgcttgccc aaccaccgcg ctcccggttt atgatcctgg ataatccggg cagatccgga
     2161 tttcgcacac cccagtcggt ttcaaccgac ttccgtctct gagacggggg tttcaacccg
     2221 tggcagggcc ggaaggccgg aatagccggg agaccgccgg tattcctggg tggtcataac
     2281 gcgaaccttg taaatgaacg gatacccgca ttctcgctaa cacacgcccc ggaggcacac
     2341 atgcagcgcg tagctatcgt cggattggga ttgatcggcg gctcgatcgg cctcggcctt
     2401 aagcgctggg ccgccgagca ggccaagggc ggccaacccc cgctccaggt catcggtttc
     2461 gacaccgacc tcgatcagca gtcctacgcc aagaagatca acgccgtcga caagaccgaa
     2521 tggcgcttgc cgaacgcgat cgccgaggcc gatatcgtca tcctcgccac gcccgtcggc
     2581 gcgatgcagg agctgctcac cgacatcgcg ccgcacctca cggagggcgc gatcgtcacc
     2641 gacgtcgcct cgaccaaggc cgatgtcgtc cggtgggccg acgagacgct gccggcgacg
     2701 gtcagcttcg tcggaggcca cccgatggcc ggcaaggcgc tcagcatcga gggcgcggac
     2761 gccgatctgt tcaagggcgc gacctgggtc gtcacgccgt cggtccgcgc ctccgagtcc
     2821 gcgctcaaga acgtgctcgg cctgatcgcc gcgctcgggg ccgaggcctt cttcgtcgac
     2881 gccgccgagc acgatgccta cgtggccggc gtcagccact tgcccttcgt cctcgccgcc
     2941 accctgacca acgcgctcgg ctccgattcg tcatggaagg acatgaagtc gctcaccgcg
     3001 ggcggcttca aggacatgac ccgcctcgcc gggggcagcc ctgtcatgca ccgcgacatc
     3061 gtgctgacca accaggacgc catcttgcgc tggatcgacc agttccaggc gcagctcagc
     3121 gccgtgcgag atctgctcgc gcgcgaatcg atcgaaggcc gcgagcacga gatcgagaag
     3181 ttcttcgaga gggcgcgcga tatccgggcc gagtggtcga cgcaggttac ccgcgagggc
     3241 gagctgctcc agcagaccgg cgccgagctg agcatggagg gcgtcggcga tcagatgtcg
     3301 cggatgttct tcggcagcct gttcaagcgg cgcatggccg aagccgaccg gctcggacgc
     3361 agccgcaacg accgtcgcaa gcttcagggc aagagcgacc aatccggcga caactcgtag
     3421 gtgtagacct gtccgaccgg ctcgccctcg cgccgcatcc ggccgcgatc cttgcgcagg
     3481 agcgggccgt accggttctc gatctggacg atccgcttgt ccggcaagtt gaccatgatc
     3541 agcccaaggg gcagcggcgt cagcgtcgcc tcgcgcagac ggtcatcgat caggcggaag
     3601 ggcgtgccgt ccaccgggtc attctgggcc agccactcgt cgatcgacga ggcgtcatcc
     3661 cgcaccacga actcgtcgaa gcgagccagt ccatggcgca gggccattgc
//
LOCUS       DASSGX010000450         3233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_622589, whole genome shotgun sequence.
ACCESSION   DASSGX010000450 DASSGX010000000
VERSION     DASSGX010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622589"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1917)
                     /locus_tag="VFI12_08030"
     CDS             complement(<1..1917)
                     /locus_tag="VFI12_08030"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET7056397.1"
                     /translation="MPAPILTVSNLSKSYGAEQIFADLSFFLGEKEHVALVGVNGAGK
                     STVLKIIAGIEEPTSGDVAPVSGLRITYLPQEARFDSDQSIREVAQEAFADAIKAAER
                     MREIEHRLGEADAAEFDVLMAEYERLHAHFEVGGGYDIEHRTEEVLVGLGFEESQFNT
                     PVHLLSGGQKTRVALARALLADPDLLLLDEPTNHLDLEMLEWLEGFLQTWGGGCLIVS
                     HDRYFLDRVTTRTLELSFGRIEDYPAPYGRYLALRAERRERRMKEYEEQQAFIARTED
                     FIRKYKAGQRSREARGRQTRLERLERVERPQDQAELAIRIQSTLRSGSQVLTSRPLTV
                     GYRSDGQDTVLVRTKDLLIERGDKVGLVGPNGAGKTTLLKTLVGEIPTLKGRFETGVN
                     VKVGYYAQAHEQLPASGTPLSVVLAAQPMGEESARNYLGRFLFTEDDVFKPVGGLSGG
                     ERSRLALAVLLLQNANFLILDEPTNHLDISARETLEELLGQFDGTVLFVSHDRFFTDR
                     IATRIWAVGDGQLTDYLGNYTDYQRALGRRQEPAPVKAPEPVAVAATKPVERRPHQSA
                     SRLQKNLTGVERDIAKLEGKLNELSDALAIASIEVDVDALARLGTEFERVQTDLDAAY
                     QRWEELSADLEAVAQ"
     gene            2042..>3233
                     /gene="lysA"
                     /locus_tag="VFI12_08035"
     CDS             2042..>3233
                     /gene="lysA"
                     /locus_tag="VFI12_08035"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HET7056398.1"
                     /translation="MLWPNTTMRSDRGELVIGGCPVNELAAQFDTPLYVFDEATLHDR
                     AERVVRAFADRNPDARVVYGGKAYLSPAIIRLFRSHGIGLDVVSGGEIYGGLKAGIPA
                     ADMVFHGNNKTADELRSALEHGVGVIVVDNEDEIDRLEEIAGQLHGRRDAVGTKVPVM
                     LRLNPGVDVHTHAKISTGIVDSKFGFPVWEDNARDAVDRLAVCRHLELIGFHMHLGSQ
                     VSTHEAMPVAIDRQVAFAAEARDRHGIVARRYSPGGGLSIAYTEAGVDADPGEWAQVI
                     TDAYSVAWEKHGLPAGTIIAEPGRWLVGPAAVALYRVGSVKRIPGVRTYIAVDGGMAD
                     NIRPSMYDASYTAEIANRNATGPCERVRIAGRFCESGDVLIEDIALPEVEVGDLIAVP
                     ASGAY"
BASE COUNT          556 a         1004 c         1077 g          596 t
ORIGIN      
        1 ctgagcgaca gcctccaggt cggcggagag ctcttcccag cgctggtagg cggcgtcgag
       61 gtcggtttgg acccgctcga actcggttcc gagccgggcg agcgcgtcga catcgacctc
      121 gatgctggca atggcaaggg cgtcgctcag ctcgttcagc ttgccttcga gtttggcgat
      181 atcccgctcg acgccggtca ggttcttctg cagccggctc gcagactggt gcggccggcg
      241 ctcgacgggc ttcgtcgccg caaccgcgac tggctccggc gccttgaccg gggcaggttc
      301 ctggcggcga ccaagcgcgc gctggtagtc ggtgtagttg ccgagataat cggtcaactg
      361 cccgtctccc accgcccaga tcctggtcgc gatccggtcg gtgaagaagc ggtcgtgcga
      421 aacgaacagg accgtgccgt caaactgccc gagcaactcc tcgagtgttt cccgggcgga
      481 aatgtcgagg tggttcgtcg gctcgtcgag gataaggaaa tttgcgttct gaaggaggag
      541 cacggccagc gcaaggcgtg accgctcgcc gcccgagagg ccgccgaccg gtttgaagac
      601 gtcatcttcg gtgaagagga agcggccgag gtagttccgg gccgactcct cgcccatcgg
      661 ctgggcggcg agcacgaccg aaagcggcgt gccactggct ggcaactgct cgtgggcttg
      721 cgcgtaatag ccgaccttga cgttgacgcc ggtctcgaac cggcccttga gcgtcgggat
      781 ttcgccgacc agcgtcttga gcagcgtcgt cttgccggcg ccgttcggac cgacgagacc
      841 aaccttgtcg ccccgctcga tcagcaagtc cttcgtccgg accagcacgg tgtcctgacc
      901 atctgagcga tagccgacgg tcaacgggcg ggatgtgagc acctgcgagc cgctgcggag
      961 cgtggactgg atgcggatcg ccaactcagc ctggtcttgc ggccgctcga cccgctcaag
     1021 ccgttccagg cgtgtttgcc ggccccgggc ctcacgagag cgctggccgg ccttgtactt
     1081 gcggatgaag tcctcggtgc gggcgataaa tgcctgctgc tcctcatact ccttcatccg
     1141 ccgctcgcgc cgctcggcgc gcagggcgag gtaccggccg tatggcgccg ggtagtcttc
     1201 gatccggccg aacgagagct cgagcgtccg ggtggtgaca cggtcgagga agtagcgatc
     1261 gtgcgagacg atcaggcacc cgccgcccca tgtttgcagg aagccctcca gccactcgag
     1321 catttcgagg tcgaggtgat tggtcggctc gtcgagcagg agcagatccg gatcggccag
     1381 gagcgcccgg gccaatgcga cgcgcgtctt ctgcccgccc gacaacaaat ggaccggagt
     1441 gttgaattgg gactcctcga agccaagacc gaccaacacc tcttcggtcc ggtgttcgat
     1501 gtcgtacccg ccgccgacct cgaagtgggc gtgcagccgc tcgtactcgg ccatcagcac
     1561 gtcgaactca gccgcgtcgg cctcacccaa ccggtgctcg atctcccgca tccgctccgc
     1621 cgctttgatt gcatcggcga atgcctcttg cgcgacctcc cgaatcgatt gatcgctgtc
     1681 gaagcgggcc tcctgcggca ggtacgttat ccgcagtccg ctgaccggcg cgacatcgcc
     1741 ggacgtcggc tcctcgatcc cggcgatgat cttgagcacg gtcgatttgc cggcgccatt
     1801 gacgccaacc agtgccacgt gttccttttc gccaagaaag aaagagagat cggcgaagat
     1861 ctgctcagcg ccgtaggact tgctcaggtt ggagacggtc agaatgggtg cgggcatgat
     1921 tggtgtgcta tagggaacgg cctcatcccg gtgatgagcg ccgacttcac agtatatccg
     1981 gcgagaatcc cgaccggatc ggaacccgat tagactatgt ccgtacacat tggagggaac
     2041 cgtgctctgg cctaacacga cgatgcgatc cgatcggggc gaactggtca tcggcggctg
     2101 cccggtcaac gagttggccg cgcagttcga cacgccgctc tacgtctttg atgaagcgac
     2161 cctgcatgac cgggccgagc gggtcgtccg cgcctttgcc gaccgcaatc cggacgcgcg
     2221 ggtcgtctac ggcggcaagg cgtatctctc cccggcaatc attcgcctgt ttcgcagcca
     2281 cggtatcggg ctggatgtcg tctccggcgg tgagatctac ggtgggctga aggcaggcat
     2341 tccggccgcg gacatggtct tccacggcaa caacaagacc gccgacgaac tgcgctctgc
     2401 cctggagcac ggcgtgggcg tgatcgtggt cgataacgag gacgaaatcg accgcctgga
     2461 ggagatcgcg ggtcaactgc acggacgtcg tgatgcggtc ggtacgaagg tccccgtgat
     2521 gctgcggttg aaccccggtg tcgatgtgca tactcacgcc aagatcagca caggcatcgt
     2581 cgattcgaaa tttgggtttc cggtctggga agacaacgcg cgcgacgccg tcgaccggtt
     2641 ggccgtctgc cggcacctcg agctcatcgg gtttcacatg catcttggat cgcaggtcag
     2701 cacgcatgag gccatgccgg tcgcgatcga tcggcaggtg gcctttgccg ctgaggcgcg
     2761 cgatcggcac ggcatcgtgg cgcggcggta ctcaccgggc ggtggtttga gcatcgccta
     2821 tacggaagcg ggggtcgacg ccgatccggg ggaatgggcg caagtgatca cagacgcgta
     2881 ttcagtagcg tgggaaaagc atgggttgcc ggcgggaacg attattgccg aacccggacg
     2941 atggctggtt ggccccgctg ctgtggcgct ctatcgtgtt ggctccgtga aacgaattcc
     3001 gggggtccgt acgtatatag ctgtagacgg tggcatggct gacaatatcc gtccctccat
     3061 gtacgacgcc agctacaccg cggagatcgc gaaccggaat gcgaccgggc catgcgaacg
     3121 ggtccggatc gccgggcggt tctgcgagtc gggcgatgtc ctgatcgagg acatcgcgct
     3181 gccggaagtc gaggttggag acctgatcgc ggtcccggct tcgggcgcgt acc
//
LOCUS       DASSGX010000451         3838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_952129, whole genome shotgun sequence.
ACCESSION   DASSGX010000451 DASSGX010000000
VERSION     DASSGX010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952129"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..1548)
                     /locus_tag="VFI12_08040"
     CDS             complement(13..1548)
                     /locus_tag="VFI12_08040"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HET7056399.1"
                     /translation="MPNVAVQDAVHPLVLALDMGTSSTRAIIFDRRGHALEGSECQLE
                     YELTTTADGGAEVDPHVLFDLVGKSIDAVMMRHPVEIAAVGVACFWHSLMGVDAKGKP
                     TTPIYLWADTRSKEDVDAIKQEFDAHELWQRTGCFVHSSYWTSKLRWLKRTQPDLVKR
                     TATWCAFSDYLLRELFGAEGTSISMASSTAMMNATTHQWDEVAIKASSVDPQQLPEIR
                     NEREPLDGLGSEYAARWPALATCPWFRGIGDGACANVGSGGIGPHRIALTVGTSGALR
                     MVQPLPAGTTPDEPESLWTYRLDDQRAVIGGAISNGGKVIDWFGELTGSAFDSTAFQQ
                     AEVMAPDAHGLTILPFLAGERAPIWSDWATGSVVGLRLATNAADLIRASMEAVSYRLG
                     LLYVDLATQAEPDHQILANGGAILRSEAWMQIIADVFQHPVIALPPEEESTARGAALL
                     ALQYTRAIDSLTDADDPAGYGRTIDPDPNRGEIYRQAMDRQDRLHDLLYAHGKPRIDG
                     DAR"
     gene            1677..2549
                     /locus_tag="VFI12_08045"
     CDS             1677..2549
                     /locus_tag="VFI12_08045"
                     /inference="COORDINATES: protein motif:HMM:NF025382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4232 domain-containing protein"
                     /protein_id="HET7056400.1"
                     /translation="MDEHNFDLFSRQFAHRTSRRALLTGAIAAGAAVIGVRRQAGATT
                     QAEGGMVFDYYQAIDRRAFHTAYKCLGTRFTSRQSLENFTAGFATTVYDDLVITNVHP
                     DPVNNRVVYDVTVTAWHTDGSIHRFTGTYTEGNEAGTRKLIDAAIREIPVKDMPPLCA
                     ASDLEATMTGDAGAGQRYGTLTATNVAAGSCVLGSIPRVTVHDASGTQVISSRQEPNT
                     TITTVTLAPGKSATLGMHWSNWCGAPVMGEPQVTVALRDEGKIAHLTGLAVPPCLSAP
                     GGESQLTVKPWQIS"
     gene            2639..3037
                     /locus_tag="VFI12_08050"
     CDS             2639..3037
                     /locus_tag="VFI12_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03618 family F420-dependent PPOX class
                     oxidoreductase"
                     /protein_id="HET7056401.1"
                     /translation="MLSDAVRDFLNERRFAVLATSNPNGTVQQSVMWYLLEGDTIVMN
                     TAKGRVKFRNIDRSNRISLCVEEGLRYVTLTGEVSFNDDQEVAQADIQRIGRRYDTEE
                     EVREEHEQNWKHQQRVTLTMPIDHVVTHGF"
     gene            3053..>3838
                     /gene="cofD"
                     /locus_tag="VFI12_08055"
     CDS             3053..>3838
                     /gene="cofD"
                     /locus_tag="VFI12_08055"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HET7056402.1"
                     /translation="MITTLAGGVGGARFADGLVQAAGADNVTVVVNTADDFELYGLRI
                     CPDLDTVLYTLAGIANPVLGWGIDGDTRATLDGVAAYGEEPWFQLGDRDFATHLLRTA
                     WLREGQTLEAVTDELRRRLGIQTPIIPMTSDPVATTIETPSGWLAFQDYFVRRRQQDA
                     VIGVSFVGIEEAGPAESALDAVRDAELIVLAPSNPIVSIGPILAIPPFHRALSDRRAP
                     AVAISPIIGGRALKGPADRMLQTLGHESTALGVARLYEGLIDGF"
BASE COUNT          662 a         1293 c         1242 g          641 t
ORIGIN      
        1 ccccgcgctg cgtcagcgtg cgtcgccgtc tattcggggc ttgccgtggg cgtagagcag
       61 gtcgtgcagg cggtcctggc ggtccattgc ctgacggtag atttcgccgc gattcgggtc
      121 tgggtcgatc gtgcggccgt acccggcggg gtcatccgcg tcggtaagtg aatcgatcgc
      181 cctggtgtac tggagcgcga gcagggcagc gcccctggcg gtcgattcct cttccggagg
      241 cagcgcgatc accgggtgct ggaagacgtc ggcgatgatc tgcatccatg cctccgagcg
      301 gagaatcgcg ccgccgttgg ctaggatctg atggtccggc tcagcctgcg tggcaagatc
      361 cacatagagc agcccaagcc ggtacgagac cgcttccatg gacgcccgga tcaggtctgc
      421 cgcgttcgtg gcgagcctga ggccgacgac tgagccggtc gcccagtcac tccagatagg
      481 agcacgctcg ccggccagga acggcaggat cgtcaaaccg tgcgcatccg gcgccatcac
      541 ttcagcctgc tggaatgcgg tgctgtcgaa ggcggagccg gtcagctcgc cgaaccagtc
      601 gatgaccttg ccgccgttgc tgatcgcccc gccgatcacg gcccgctgat cgtcgagccg
      661 gtaggtccag agcgattccg gctcgtcggg cgtggtcccg gccggcaagg gctggaccat
      721 ccggagcgcg cccgaggtgc cgacggtcaa tgcgatccgg tgcggtccga tcccgcccga
      781 gcccacattc gcgcatgctc catcgccgat gccccggaac cacgggcagg tcgccagtgc
      841 cggccagcgc gcggcgtact ccgagccgag accgtcgagc ggctcgcgct cgttccggat
      901 ctccggcaac tgctgagggt cgacgctgga ggccttgatt gccacctcgt cccactggtg
      961 cgtcgtggcg ttcatcatcg cggtcgatga ggccatcgag atcgacgtgc cctcggcccc
     1021 gaacagctcg cgcagcagat aatcgctgaa ggcgcaccag gtcgcggtcc gcttgaccag
     1081 atccggttgc gtccgcttca gccagcgcag cttgctggtc caatagctgg aatggacgaa
     1141 gcagccggtc cgttgccaga gctcgtgcgc gtcgaactcc tgtttgatcg cgtcgacatc
     1201 ctccttcgag cgcgtgtcgg cccagaggta gatgggggtc gtcggcttac ccttcgcgtc
     1261 gacgcccatc agcgaatgcc agaagcaggc gacgccgacc gccgcgatct cgacggggtg
     1321 gcgcatcatg acggcgtcga tcgacttgcc gacgaggtcg aacagcacgt gcgggtcgac
     1381 ctccgcgccg ccgtcggccg tcgtcgtcag ctcatattcc agctggcatt cgcttccctc
     1441 gagggcgtgg ccgcgccggt cgaagatgat cgcccgggtc gacgaggtgc ccatgtcgag
     1501 ggcgaggacg agcgggtgga cagcatcctg gacggctaca ttcggcacgc actcctcctc
     1561 ggctcgtaat tgacccgagt atacggagcg cgagcatgtc gccggtaaac cgaaaccagc
     1621 atggctcatc ccggcgttta tgtaagtgga cattggccca cagaaacgga acagccatgg
     1681 atgagcacaa tttcgatctt ttctcccggc agttcgctca tcgcaccagc cgccgcgccc
     1741 ttctaaccgg cgcgatcgcg gccggcgccg ccgtgatcgg cgtccggcgg caagcgggtg
     1801 caacgaccca ggccgagggc ggcatggtct tcgactacta ccaggccatc gaccgccgcg
     1861 cgttccacac cgcgtacaag tgcctgggaa cccggttcac ctcccggcaa tcgctcgaga
     1921 acttcaccgc gggcttcgca accactgtct acgacgacct cgtaatcacc aacgtccatc
     1981 ctgacccggt caataaccgc gtggtctacg acgtgaccgt caccgcctgg cataccgacg
     2041 gctcgattca ccgcttcacc gggacgtata ccgaaggcaa tgaagcagga actcgcaagc
     2101 tgatcgatgc cgcgatccgg gagattccgg tcaaagacat gccgcccctc tgtgccgcga
     2161 gcgatctcga agcgacgatg acgggcgatg cgggcgcggg gcagcgctat ggcaccctga
     2221 cggcgaccaa cgtcgcagcc gggtcctgcg tactcggcag tattccccgg gtaacggtcc
     2281 acgacgcgtc cgggacccag gtcatctcgt cccggcagga gccgaacacc acgatcacga
     2341 cagtaacgct cgcgccgggc aagagcgcga cgctcgggat gcactggtcg aattggtgcg
     2401 gtgcgccggt catgggagag ccgcaggtga cggttgccct tcgtgatgag ggcaagatcg
     2461 cgcacctgac cggtctcgcc gtgccgccct gcctgagcgc ccccggcggt gaatcgcagc
     2521 tgaccgtcaa gccctggcag atcagttagg cacgcaatcg gttgacggct ccggcgggct
     2581 ctcctagcat tgcttcccta attgaagcgt tatgcctgca aggggagatt cctgtctgat
     2641 gttgagcgat gccgtccgcg atttcctgaa cgagcgccga ttcgccgtgc tggcgacctc
     2701 caatccgaat ggcaccgtcc agcaatcggt gatgtggtac ctgctcgaag gtgacacgat
     2761 cgtgatgaac acggccaagg gccgggtcaa gttccgcaac atagaccgtt ctaaccggat
     2821 ctccctctgc gttgaagagg gcctgcgtta cgtcaccctg accggcgagg tcagcttcaa
     2881 cgacgaccag gaggtcgcgc aagcggacat ccagcgcatc ggacggcgct acgataccga
     2941 ggaggaggtg cgggaggagc acgagcagaa ctggaagcac cagcagcgcg tcaccctcac
     3001 catgcccatt gaccacgtcg tgacgcacgg gttttagagc ggcaaccgat tcatgatcac
     3061 cacactcgcc ggcggggtcg gcggcgcgcg ctttgccgat ggactggttc aggcggccgg
     3121 cgcggacaac gtgacagtcg tcgtcaacac cgccgacgac ttcgagctct acgggctgcg
     3181 catctgcccg gacctcgaca ccgtgctcta caccctggcc gggatcgcca atccagtcct
     3241 gggctggggc atcgacggcg acacgcgggc gacgctggac ggcgtcgccg cctacggcga
     3301 ggaaccgtgg ttccagctcg gcgaccgcga tttcgcgacg cacctcctcc gtaccgcctg
     3361 gctgcgcgag ggacagacgc tcgaagcagt cacggacgag ctccggcgac ggctcggcat
     3421 tcagacgccg atcatcccga tgaccagcga tccagtcgcg accacgatcg agacgccgtc
     3481 agggtggctc gcgttccagg actacttcgt acgccgccgt cagcaagacg cggtgatcgg
     3541 cgtctcattc gtcggcatcg aagaggccgg cccggccgag tcggccctcg atgcggttcg
     3601 ggacgcggag ctgatcgtgc tcgccccgtc caacccgatc gtcagcattg gcccgatcct
     3661 tgccatcccg ccgttccacc gggccctctc ggaccggcgc gcgccggcgg ttgcgatcag
     3721 cccgatcatc ggcggccgcg ccttgaaggg gccggccgat cggatgctgc agacactcgg
     3781 ccacgaatcc accgcgctcg gcgtggcccg gctctacgaa gggctgatcg acgggttc
//
LOCUS       DASSGX010000452         6389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1605041, whole genome shotgun sequence.
ACCESSION   DASSGX010000452 DASSGX010000000
VERSION     DASSGX010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6389
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1605041"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1416
                     /gene="pckA"
                     /locus_tag="VFI12_08060"
     CDS             <1..1416
                     /gene="pckA"
                     /locus_tag="VFI12_08060"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF006820.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HET7056403.1"
                     /translation="SHLAEHGPLVVETGVHTGRSARDKFVVEDGVTGETVWWGQVNQQ
                     MSPEHFAVLQKDVLAHLSAKPTITIDLRAGADLRYGIPVRLVTEHAWAALFARNLFLP
                     PDAGAMLEPFRILHAPTFDANPVKYGTRSNTVIAISFSMRTVIIAGTHYAGEIKKSIF
                     SVLQYLLPDQGVATMHCAANMGEKGDVALFFGLSGTGKTTLSTDKDRILIGDDEHGWS
                     KRGVFNFEGGSYAKVINLSPVAEPGIWNASHRFGTVLENVVMDPETRALDLDDATLTE
                     NTRSAFPLGFIPGASDTGLGGHPRHIVFLTADAFGVLPPVAKLAPEQAHDWYLAGYTS
                     KLAGTEKGVTEPAATFSACFGDAFLSHPPRRYAELLDARIAEHNPSLWLVNTGWTGGA
                     YGTGERISIAHTRAIIRAILAGELEDVQLETEPIFGLNLPTRVKDVPRDILRPWHGWS
                     NYGAYEKAASRLATDFRNAIR"
     gene            1574..1996
                     /locus_tag="VFI12_08065"
     CDS             1574..1996
                     /locus_tag="VFI12_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056404.1"
                     /translation="MNATLGRRVIRIDGDVIARRVIGGIWLAGAAFNAGWTLRQDGPW
                     TWLEESRVPVYRWFFGEVAAANPAPWTVALIAGEITLGLLTLSQRWGRRGLIAGAAFS
                     AFLFSLGTPYTLMMGGYALLLAWLARRTGRRPFGTAPG"
     gene            complement(2393..2854)
                     /locus_tag="VFI12_08070"
     CDS             complement(2393..2854)
                     /locus_tag="VFI12_08070"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7056405.1"
                     /translation="MNVIDIRGFNEDAVVGPQNGASRLFIWCTSVLAGEVVALHRHLG
                     EELTRVLYGKLRFHVSGETREIGPGEVVIVEPGAVHGYVAVEDAEIEVYGEVGSGVFV
                     PNETSDGKVRFEEVFIRDVPWSRTPPDDSQYITRAEQLERYRASYAAAPFA"
     gene            complement(2937..3743)
                     /locus_tag="VFI12_08075"
     CDS             complement(2937..3743)
                     /locus_tag="VFI12_08075"
                     /inference="COORDINATES: protein motif:HMM:NF016533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HET7056406.1"
                     /translation="MIFHDYLERWDLTPDGDLVITPTSHLLPVRRDGLPAMLKIATEP
                     EERRGGNLMTWWNGDGAARVLAHGESALLLDRATGNRFLIEMARSGQDDEATRIICAV
                     AARLHTPRGTHPPYLIPLAGWFRELAPAAERHGGVLRQSAEIARELLADQHDITVLHG
                     DIHHGNILDFGPRGWLAIDPKGLIGERSFDFANIFCNPDSDTVTAPGRLARQATVVAE
                     SAGIDRDRLLRWILAYAGLSAAWFLADAMEADARLLMTVAEIAAAEIDRR"
     gene            complement(3740..4693)
                     /gene="pip"
                     /locus_tag="VFI12_08080"
     CDS             complement(3740..4693)
                     /gene="pip"
                     /locus_tag="VFI12_08080"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HET7056407.1"
                     /translation="MQYPPIEPYATGYLPVSDGNEIYWEASGNPDGKPALYLHGGPGS
                     GVGTGYRRNFDPAQYLIVSLDQRGCGRSRPLVSNSLANLHTNTTQALIADLEALREHL
                     GIDRWLVTGLSWGTTLALAYAQAHPDRVTELVLGAVTSTSPDEVEWITESVGRIFPEA
                     WDRFAAASGREPGQRVIDAYSERITDPDPAVRAQAATDWCAWEDVHVSLDPNHAPFPD
                     FEDPEFRAVFATLVIHYWKHSGFAGEGGLLAGMDRISHIPGVLIHGRMDVSGPLVTAW
                     ELHKRWPGSELIVVEGEGHGGPEMISELRRAYARFAQNTSS"
     gene            complement(4767..>6389)
                     /locus_tag="VFI12_08085"
     CDS             complement(4767..>6389)
                     /locus_tag="VFI12_08085"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HET7056408.1"
                     /translation="YVTWENIRIQNAPRAGLRVSMSSHVTVRNNIFANNQRWGLFTDF
                     SDDLLIEGNESFGATIEHGIYLSNSGDRPVIRGNVIHDNHANGLHMNGDISMGGDGTI
                     TSPVVEGNYIFSNGVGGGSGINMDGVERAMIRNNVIIDAHASGISMYQIDGGICSRNN
                     TLYHNTVVIAADGRWAINIPNIGCTGNQLIANILYSNHPYRGAINIVDGARSGFTSNY
                     NVLEGVFSTDGGDTTLTFAQWKALGYDANSKISTPSSLFVNATSDYHLRSGSPAISIG
                     TTLTSVKIDFDGGARPQGASSDAGAFEAASPADCRLAPTSGVVYSTLTVTCTDFQPDE
                     YVTLTWDRSDIARKRASGGGNATVSFTVPRGVTGRHLVKATGDLGTVVPSRGYRIIPS
                     LTLSPTGGVIGQKISATLRGFAAGEMITLRFRTAPGSSRTVARNIITSSTGTASATFL
                     VPSGSVGTYSIEANGSAGGFASARFTRILGTAEDPTATPTATPTPTNTSTPMPPESTV
                     TAEPTDSPPPTETPDQPSPTVDDPVPAGTPIA"
BASE COUNT         1057 a         1963 c         2245 g         1124 t
ORIGIN      
        1 tcgcacctcg ccgagcatgg cccgctcgtc gtcgaaaccg gcgtccacac cggccgctcc
       61 gccagggaca agttcgtcgt cgaggacggg gtcacgggtg agacggtctg gtggggccaa
      121 gtcaaccaac agatgtcgcc cgagcacttc gccgtgctgc agaaggacgt cctcgcgcac
      181 ctcagcgcca aaccgacgat cacgatcgat ttgcgggccg gggcggacct tcgctacggc
      241 atcccggttc gattggtgac ggagcatgcc tgggcggcgc tctttgcccg caacctgttc
      301 ctgccgccgg acgccggcgc gatgctcgag ccgttccgga tcctgcacgc gccgaccttc
      361 gatgccaatc ccgtgaagta cggcacccgc tcgaacaccg tcatcgccat ctccttctcg
      421 atgcggaccg tgatcatcgc cggcacgcat tacgcgggtg agatcaagaa gtcgatcttc
      481 tccgtgctcc agtacctgtt gccagaccag ggcgtcgcca caatgcactg tgcggcgaac
      541 atgggcgaga agggcgatgt ggccctcttc ttcggcctct ccggaaccgg caagacgacg
      601 ctctcgaccg acaaggaccg gatcctgatc ggcgacgacg agcatggctg gtcgaaacgg
      661 ggcgtcttca atttcgaggg cggctcctat gccaaggtga tcaacctttc gccggtggcc
      721 gagccgggaa tctggaacgc cagccaccgg ttcgggaccg tgctcgagaa cgtggtcatg
      781 gatcccgaaa cgcgagcgct cgacctcgac gatgcgacgt tgaccgagaa tacgcgctcc
      841 gcattcccgc tcggcttcat cccgggcgca tcggacaccg gactgggcgg gcatccgcgt
      901 cacatcgtct tcctgacggc cgatgccttc ggcgttctgc cacccgtcgc caaattggcg
      961 ccggagcagg cgcacgattg gtacctggcc ggatatacgt cgaagctggc cggcaccgaa
     1021 aaaggggtga ccgagccggc agcgaccttc tccgcatgct tcggcgatgc gttcctgtcg
     1081 cacccgccgc ggcgctatgc ggaattactg gatgccagga tcgccgagca caatccgtcg
     1141 ctctggctgg tgaacaccgg ctggacgggt ggtgcgtacg gcacgggcga gcgaatctcg
     1201 atcgcccaca cccgcgcgat catccgcgcg atcctcgctg gcgagctgga ggatgtgcag
     1261 ctcgagaccg agccaatctt cggtctcaat ctgcccacgc gggtcaaaga cgtcccgcgc
     1321 gacatcctgc ggccctggca tgggtggtcc aactacggcg catacgagaa ggcggccagc
     1381 cgattggcga ccgacttccg gaacgcgatt cggtaagcaa accactcaag acaagaccgg
     1441 accccgaagc tgccatcgct ggcgacttcg gggtccgcgt gtctgtcacg aattcctgac
     1501 cttgcatgca aattacgggt cgcggcagtg acacgccggc cgacatcctt gaccggggag
     1561 ccggaggata gcaatgaacg cgacactggg gcgacgagtg atccggatcg atggcgacgt
     1621 gatcgcgcgg cgggttatcg gcgggatctg gctggccggc gcggcgttca acgccggctg
     1681 gacactgcgg caggatgggc cgtggacatg gctggaggaa tcgcgcgtac cggtctaccg
     1741 ctggttcttc ggcgaggtgg ctgccgcgaa cccggcgccg tggacagtcg cgctgatcgc
     1801 cggcgagata acactgggcc tgttgacgct cagccagcgc tggggccggc gcgggttgat
     1861 tgcgggcgcg gcgttcagcg cgttcctgtt ctcgctcggc acgccgtaca cgctgatgat
     1921 gggcggctac gcgctgctgc tggcgtggct cgcgcgccgg acggggaggc gaccgttcgg
     1981 caccgctccg ggttgatctt gattcgctgg tggcgggtcc ccatacccaa gcggccaggc
     2041 agcgccgcct gggttaaggg cccgggctac ctaacgatac gtcctctgaa gggactaaaa
     2101 gcggagccgg cttcagccgc cttattgctg ctcagccccg gccttcagcc cggggcgatg
     2161 attacggccc ggtttccagg gaacgggccg tggggcagag cagccgggcg gggccgcccg
     2221 gggctacatt gtcgagagag cgcctgccta agcctcttta gtgggcttct gtttgttcag
     2281 ccccggcctt cagctcgggg cgggagtgac ggcgcggtca ccatggagcg cgactctgga
     2341 tagcgcaagc gcgagagccg cccgaggaag tcaagtcgct tggaaccctg cgtcaggcga
     2401 agggggcggc ggcgtagctg gcgcggtagc gctcgagctg ctcggcgcgg gtgatgtatt
     2461 ggctgtcgtc gggcggggtg cggctccagg ggacatcgcg gatgaagacc tcttcgaacc
     2521 ggaccttccc gtcgctggtc tcattcggca cgaacacgcc ggatccgacc tcgccgtaga
     2581 cctcgatttc ggcgtcctcg acagcgacat aaccatggac ggcgcccggc tcgacgatca
     2641 cgacctcgcc cggaccgatc tcacgcgtct cgccggacac atggaaacgg agcttgccgt
     2701 aaaggactcg ggtcagctcc tcgccgaggt ggcggtgcaa ggcgacgact tcaccggcca
     2761 gtacggaagt gcaccagatg aaaaggcggc tcgccccgtt ttgcggaccg actacggcgt
     2821 cttcattgaa gcctcgaata tcgatgacat tcatcgcagg cccctttggt gctgtccaat
     2881 tcgtgcgcag ccctagcata tacccgaacc cggccgaggt cgataggcaa accggcttat
     2941 cggcggtcga tttcggccgc ggcgatctcc gcgactgtca tcagcagacg cgcgtccgct
     3001 tccatcgcat cggcgaggaa ccaggcggcc gaaaggccgg cgtaggcgag aatccagcgc
     3061 aggaggcggt cgcggtcaat gccggcggat tcggcgacca cggtcgcctg ccgggcgagg
     3121 cggccgggcg cggtcacagt gtcggaatcc gggttacaga agatgttggc aaagtcgaag
     3181 ctgcgctcgc cgatcagtcc cttggggtcg atcgccagcc agccacgcgg tccgaaatcg
     3241 aggatgttgc cgtggtggat gtcgccgtgc aggacggtga tgtcgtgctg atcggccagc
     3301 agctcgcggg caatctcggc ggactgacgg aggacgccgc catgccgctc ggcggccggc
     3361 gccagttccc ggaaccaccc ggcgagcggg atcagatacg gtggatgtgt cccgcgcgga
     3421 gtgtggagcc gggccgcgac agcgcagatg atccgcgttg cctcgtcgtc ctggccggag
     3481 cgagccatct cgatcaggaa gcggttgccg gttgctcgat cgagcagcag ggccgattcg
     3541 ccgtgagcaa ggacacgggc cgcgccatcg ccgttccacc aggtcatgag gttgccgccg
     3601 cgccgctctt cgggctcggt cgcgatcttg agcatggccg gtaggccgtc tcggcggacc
     3661 gggaggaggt ggctggtcgg cgtgattacc agatcaccat cgggggtcag gtcccagcgc
     3721 tcaaggtaat catggaagat cacgacgatg tgttctgggc gaagcgggcg tacgcgcggc
     3781 gcagctccga gatcatctca gggccaccgt gaccctcacc ctcgacgacg atcagttcgc
     3841 tgccgggcca gcgcttgtgc agctcccagg cggtgacgag cgggccgctg acgtccatcc
     3901 ggccgtgaat caggacgccg gggatgtgcg agatgcggtc catgccggcc agcaggccac
     3961 cttcgccagc gaaaccgctg tgcttccagt agtggataac gagcgtcgcg aagacagcgc
     4021 ggaactccgg atcctcgaaa tccgggaacg gcgcgtggtt gggatcgagc gagacgtgga
     4081 catcttccca cgcgcaccag tccgtcgccg cctgcgcgcg gacggcggga tcgggatcgg
     4141 tgatgcgctc ggagtaggcg tcgattacgc gctggcccgg ctcgcgacca gaggcggctg
     4201 caaagcggtc ccacgcctcg gggaagatgc ggccgaccga ctcggtgatc cactccacct
     4261 cgtccgggct ggtcgaggtg actgcgccga ggacgagctc ggtaacgcgg tccggatggg
     4321 cttgggcgta ggcgagggcg agcgtcgtac cccacgagag gccggtcacg agccatctgt
     4381 cgatgcccag gtgctcccgc agcgcttcga gatcggcgat cagggcctga gtcgtgttcg
     4441 tgtgtaggtt ggcgagcgag ttgctgacga gggggcggct gcggccgcac ccgcgctggt
     4501 cgagggagac gatcaggtac tgcgcggggt cgaagttgcg gcggtagccg gtgccgacgc
     4561 cgctgcccgg gccgccgtgc aggtagagcg ccggcttgcc atccgggttg ccggacgctt
     4621 cccagtagat ctcgttgccg tcgctgaccg ggaggtagcc ggtcgcgtag ggctcgattg
     4681 ggggatattg catggccatc ggctcccgca ccagggatga agtcggtccc gtgcgtgcga
     4741 atggtaacgg tgcgagagga acgggctcag gcgatcggtg tgccagcggg tactggatca
     4801 tcgactgtcg gcgatggctg atccggcgtt tcggttggtg gtggggagtc cgtaggctcg
     4861 gccgtgacgg ttgattcggg cggcatgggg gtgctggtgt tcgtgggcgt tggcgtcgcc
     4921 gttggggtgg cggttgggtc ttcggcggtg ccgaggatgc gggtgaacct ggccgatgcg
     4981 aaaccgccgg cgctgccgtt ggcttcgatc gagtacgtgc cgacggagcc gctcgggacc
     5041 aggaacgtgg ccgaagcggt gccggtgctg gaggtaatga tgttcctggc gaccgtgcgg
     5101 gagctcccgg gcgcggtccg gaagcggagc gtgatcatct cgccggccgc gaagccgcgc
     5161 agtgtcgcgc tgatcttctg accgatcacg ccgccggttg gggagagcgt cagcgaggga
     5221 atgatccgat acccacgtga cggcacgacc gtacccaaat cgccggtggc cttgaccaga
     5281 tgtcgtcccg tcactccccg gggaacagtg aaggagaccg tcgcattgcc gccgccactg
     5341 gcccgcttcc tggcgatatc ggatcgatcc caggtcagcg tgacgtattc gtccggttgg
     5401 aagtccgtgc aggtcacggt gagcgtcgaa tagacgactc cggaggtcgg cgcgaggcga
     5461 cagtcggccg gcgaggcggc ctcaaatgcg ccggcatcgg aggatgcgcc ttgcggccgg
     5521 gctccgccgt cgaaatcgat cttcactgag gtcagggtcg tgccgatgct gattgctggc
     5581 gagccggacc tgaggtggta gtcgctggtc gcattgacga acagcgatga gggcgtcgag
     5641 atcttcgagt tggcgtcgta gccgagcgcc ttccactggg caaaggtcag ggtcgtgtcg
     5701 ccgccgtcgg tgctgaagac gccctcaagg acgttgtagt tgctggtaaa accggagcga
     5761 gcgccgtcga cgatgttaat tgcgccgcga taggggtggt tgctgtagag gatgttggcg
     5821 attaactggt tgccagtgca gccgatgttc gggatgttga tcgcccagcg accgtccgcc
     5881 gcgattacga ccgtgttgtg atagagcgtg ttattccggg agcagatgcc gccgtcgatc
     5941 tggtacatcg agatgccgct ggcgtgggca tcgatgatca cgttgttgcg gatcatggcc
     6001 cgctcgaccc cgtccatgtt gatcccggag ccgccgccca ctccgttact gaatatgtag
     6061 ttgccctcaa caacaggact cgtgatcgtg ccgtcgccgc ccatcgagat gtcgccgttc
     6121 atatgcaggc cgttggcatg gttgtcgtgg atcacgttgc cgcggatgac cggccgatcc
     6181 ccgctgttcg agagataaat gccgtgctcg atggtcgcgc cgaaggactc gttgccctcg
     6241 atcaggagat cgtcgctgaa gtcggtgaag agcccccagc gctggttgtt ggcgaagatg
     6301 ttgttccgga ccgtgacgtg gctggacatg gagacgcgca ggccggcccg gggcgcgttc
     6361 tggatccgga tgttctccca cgtcacgta
//
LOCUS       DASSGX010000453         3670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_952228, whole genome shotgun sequence.
ACCESSION   DASSGX010000453 DASSGX010000000
VERSION     DASSGX010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952228"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VFI12_08090"
     CDS             complement(<1..498)
                     /locus_tag="VFI12_08090"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7056409.1"
                     /translation="MNAILRAFAAGPEAPRIADSEVVDRLYRSNRVRVMLAITVGYGL
                     IYMCRLAIGIVKKPLIDGGIFTPAELGTIGSALFYTYAVGKLTNGFLADHANLRRFLA
                     LAFVLTALCNVAMGFATSLVVATVLWGLNGWFQSFGAPGGAVALTAWFSNRERGRAYG
                     VWSTAH"
     gene            complement(551..3028)
                     /locus_tag="VFI12_08095"
     CDS             complement(551..3028)
                     /locus_tag="VFI12_08095"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HET7056410.1"
                     /translation="MTRKLHALQVLLFSTALFSAQAALAQQPTSPDQEPAAETAGEGD
                     AKAEGQPAVPTNEGLESPDNRTLEEIVVIGHQIYRNRTDTIAPELTFGQEFFQKFEPT
                     SVGDSLKRVPGVAFTSDIGEYDAPALRGLGAGFTQILVNGRPIPGAGNDRSVFVDRIP
                     AEIIDRIEIIRSPTADLDSQGIGGTINIILKDGTSLPPGVIARAGVLYFPDGKDTFKG
                     SGAISWSGRNTAETVAWSLTVDAQQRYNPKLTRQEVFNDDVPGFEESSTGIDLFRPFD
                     REGSIAVERREELDTRRSFDLSLNGDITFEVGAQSKLRFDGFFIRTRRTDTEQQINLE
                     RDEVDDPYEIDDVQLSKEPFKQANFGLSGLYEAKFGEGMSVETQLRYSQFKEKSSNNA
                     WELDVDDDELDTLRETDLNFNDFDSELIEINALDTTDREFSGDASFKKEWANWSIKVG
                     TAGKLKNRRFGQTVGEDLNDEEDASLVESLFRYRENRLDGFALAEFKLGGGAKAQAGV
                     RAEYTKTRQKIRQDITEDEEESASSSEFHLNPSAHLQVPLGDGPQLRVSVARTVRRPN
                     IDQVVPFSQVDDPEDNDITVGNPDLKFETAWGVDVGLEQRIQRGVVGVNFFYRRVNNL
                     INLVNTGIPSDPEADPESEEGRARIYTFDNVGRGKVYGFEFDLSAPLSFIGLDNTGIF
                     ANFTRLWSKRTEPNTGMKVPFDGQPKYVYNFGLTQDFPKLGASAGFSFRKQGESISTF
                     LGEEEHQFYEGNLEAYVEKRIGSNFVLRLSGNNLLDARSLQYERNFDGDTGLDIIENQ
                     QAGNVDNFELEHEETSRQIMLTARLVF"
     gene            3189..>3670
                     /locus_tag="VFI12_08100"
     CDS             3189..>3670
                     /locus_tag="VFI12_08100"
                     /inference="COORDINATES: protein motif:HMM:NF014396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016158 - 3-phytase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytase"
                     /protein_id="HET7056411.1"
                     /translation="MHAFTRTLAVAGLLAGAAAAAQTAPAVPTVDGVGTSPTQQGGAN
                     RAVLILDGQSPLIVGTAELAGLELYDTDGKRIGAVPAGEAVGVDVRYGWPSAGSGATL
                     VAADATTNALRFYRPLNGSLGEVSARPVPLNIAVEGTCLYRSTQDGSLYAFALGDGGE
                     "
BASE COUNT          647 a         1135 c         1161 g          727 t
ORIGIN      
        1 gtgggccgtg ctccacacgc cataggctcg cccccgctcc cggttcgaga accaggcggt
       61 gagcgcgacg gctccgcctg gcgctccgaa gctctggaac catccgttga gaccccagag
      121 gaccgtcgct acgacgagcg aggtggcgaa gcccatggcg acgttgcaaa gcgccgtcag
      181 gacgaaggcc agcgcgagga aacgccgcag gttcgcatgg tcggccagga agccgttggt
      241 gagctttccg actgcatagg tgtagaagag cgcggacccg atcgtcccga gctccgcagg
      301 cgtgaagatg ccgccgtcga tcagcggctt cttcacgatg ccgatcgcca gccggcacat
      361 gtaaatgagc ccgtaaccga ccgtgattgc gagcatcacg cggacgcggt tgcttcggta
      421 gaggcgatcg acgacttcgg agtccgcgat cctcggagcc tcgggcccgg ccgcgaatgc
      481 acgaaggatg gcgttcaaga cacttcgccc gtcagtggtt caacgcctgc acaggaccac
      541 gccaccccgc ttaaaaaaca agacgcgcag tcaacatgat ttgccgtgac gtctcttcgt
      601 gctccagctc gaaattgtcg acgttgccgg cctgttgatt ctcgatgatg tcgagacccg
      661 tgtcgccgtc gaaattgcgc tcatactgaa ggctgcgcgc gtcgagcagg ttgttgccgc
      721 tcaggcgaag gacgaagttg ctgccgatgc gcttctcgac ataggcttcc agattgccct
      781 cgtagaactg gtgctcctcc tcaccgagga aggtgctgat cgattctccc tgcttgcgga
      841 aactgaagcc ggctgatgcc ccaagcttcg ggaagtcctg agtcaggccg aagttgtaga
      901 cgtatttcgg ctgcccatcg aacggcacct tcatcccggt gttcggctcg gtgcgcttcg
      961 accacaggcg ggtgaagttg gcgaagatgc cggtgttgtc gaggccgatg aacgatagcg
     1021 gcgcgctcag gtcgaactcg aagccgtaga ccttgcccct gccgacattg tcgaaggtgt
     1081 agatccgggc gcggccctct tcgctttcag gatcggcctc cggatcggac gggatgccgg
     1141 tgttcaccaa attgatgagg ttgttcaccc ggcggtagaa gaaattgact ccgacgacgc
     1201 cgcgctggat ccgctgctcg agcccaacgt ccacgcccca ggccgtctcg aatttcaggt
     1261 ccgggttgcc gaccgtgatg tcattgtctt ccgggtcgtc gacctgactg aacggtacga
     1321 cctgatcgat gttcgggcgc cggaccgtgc gtgcaacgct gacccgaagc tgcgggccgt
     1381 cgccgagcgg cacctgcagg tgcgccgacg ggttgaggtg gaactcgctg gatgaagcgg
     1441 attcctcctc gtcctcggta atgtcctgac gaatcttctg ccgggtcttg gtatattcgg
     1501 cacggacgcc cgcctgcgcc tttgcgccgc cgcccagctt gaactccgcg agagcgaagc
     1561 cgtcgagccg gttctcgcga tagcggaaca ggctttcgac cagcgacgcg tcttcctcgt
     1621 cattgaggtc ctcaccgacc gtctggccga agcgccggtt cttgagcttg ccggcggtgc
     1681 cgaccttgat gctccagttc gcccattctt tcttgaacga agcatcgccg gaaaactcgc
     1741 gatcggtcgt gtcgagcgca tttatctcga tcagctccga gtcgaaatcg ttgaagttga
     1801 ggtccgtctc acgaagcgtg tcgagttcgt cgtcatcgac gtcaagctcc caggcgttgt
     1861 tcgagctctt ctccttgaac tgcgagtagc gcaactgcgt ctcgacgctc atgccctccc
     1921 cgaatttggc ctcgtaaagg cccgacaggc cgaagttcgc ctgcttgaac ggctctttcg
     1981 agagttggac atcgtcgatc tcgtacgggt cgtcgacttc gtcccgctcg agattgatct
     2041 gctgctcggt gtcggtgcgg cgcgttcgga tgaagaagcc gtcgaaccta agcttgcttt
     2101 gagcgccgac ttcgaaggtg atgtcgccat tcagtgacag gtcgaagctt cgccgcgtgt
     2161 cgagctcctc ccggcgctcg accgcgatcg atccctcacg gtcgaacgga cggaacaggt
     2221 cgattccggt tgagctctcc tcgaaccccg gcacatcatc attgaaaact tcctgccggg
     2281 tgagcttcgg attgtagcgc tgctgtgcgt cgaccgtcag cgaccaggcg acggtttcgg
     2341 cagtattgcg tcccgaccag gaaatggcgc cggagccttt gaaagtgtcc tttccatccg
     2401 ggaaatagag gacccccgca cgggcgatca cgccgggggg aaggctcgtg ccgtccttca
     2461 ggatgatgtt gatcgttccg ccgatgcctt ggctgtcgag gtcggcggtc ggcgatcgga
     2521 tgatctcgat ccggtcgatg atttccgcgg gaattcggtc gacgaagacc gaccggtcgt
     2581 tccctgcgcc cgggatgggc cgtccattga cgaggatctg cgtgaagcca gcgccgaggc
     2641 cgcgaagggc gggcgcatca tattcgccga tgtcgctggt gaaggcgacg cccggaacgc
     2701 gtttcagcga gtcgccgacg gaggtcggct cgaacttctg gaagaattcc tgcccgaagg
     2761 tcagctcggg cgcgatcgtg tcggtgcgat tgcgatagat ctggtgcccg atgacgacga
     2821 tttcctcgag ggtacgattg tcgggagact cgagaccctc gttcgtaggc accgcgggtt
     2881 ggccctcggc cttggcatcg ccctccccgg cggtctcggc ggcaggttcc tggtccggac
     2941 tcgtcggctg ttgagccaac gccgcctggg cggaaaacaa ggcggtggag aaaagaagca
     3001 cctgcaaagc atgcagcttg cgcgtcatac gagccccctc atgtccccgt gcgtctctct
     3061 cccgacgagg aacgatttcg ataattgaac agaaaattcc agaatgcaac aagtttggaa
     3121 ttgtccttgc acgcgaattg cccgccagtt agctacggtg caattgttac ggcgtggaga
     3181 cgaaggtcat gcatgctttc acgcggaccc tggccgttgc cgggctcctg gccggagccg
     3241 cggctgctgc gcaaaccgcg cctgccgtgc ccacggtcga cggcgttgga acatcgccga
     3301 ctcagcaagg cggggccaat cgcgccgtgc tcattctcga cggccaaagc ccgctgatcg
     3361 tcggcacggc cgaacttgcg ggtcttgagc tttacgacac tgacggcaag cgcatcggag
     3421 cggtgccggc cggtgaagcg gtcggggtgg acgtccgcta cggctggccg agcgctggat
     3481 cgggcgcgac cctcgtcgcc gccgatgcaa ccacgaacgc cctgcgcttc taccgcccgc
     3541 tgaacggctc cctcggcgag gtcagcgccc gccccgtacc gctgaatatc gcggtcgagg
     3601 ggacgtgcct ttaccggagc acgcaggacg gatcgctcta tgccttcgcg ctcggcgacg
     3661 gtggagagat
//
LOCUS       DASSGX010000454         2003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_79440, whole genome shotgun sequence.
ACCESSION   DASSGX010000454 DASSGX010000000
VERSION     DASSGX010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79440"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            340..1152
                     /gene="aroE"
                     /locus_tag="VFI12_08105"
     CDS             340..1152
                     /gene="aroE"
                     /locus_tag="VFI12_08105"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HET7056412.1"
                     /translation="MIGDPVAHSLSPVFQQAAFDALGIPARYERWHTPAQELPARIGS
                     LRSPGFFGANVTVPHKVAVAGLVDTLSETAQRLGAVNTVINRGGRLHGDNTDLYGFMR
                     SLLTVRPSVERDRVLIIGAGGAARAVVAAVAEIGVTGVTIANRSRDRAEQLLANLRIG
                     AAEIIPLTDWDLMRAIRDHSVIVNATSIGWNDDQTVLAPELLDAVDPQGLIADLTYRD
                     TPLLVQAKARGLRTLDGLPMLVHQGARSFELWTGRDAPIDVMMAAAERAREL"
     gene            1159..1875
                     /locus_tag="VFI12_08110"
     CDS             1159..1875
                     /locus_tag="VFI12_08110"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HET7056413.1"
                     /translation="MLMAAVVAGVVGLFLASVLWRLARRQALRKPLFAGRESESLAAW
                     LPLWGLGQSRIEAETGEERRPWQAVFEVATAAYFAVLGWRFGFSIDLLMVVCFSIPLL
                     VIGLVDFWTRLIHTNVIFLGVALGWVFAVLDGGRGLLDSVIGMAVGGAVFIFFFLAAI
                     LIYRNIRVVPFGLGDVYLAAMIGAMVRIDRVMQALLIGIFLAGIILAALLAARVLSRK
                     QAVAYGPYLCLGALITLMLK"
BASE COUNT          301 a          605 c          717 g          380 t
ORIGIN      
        1 ccgatctccg ggcgcgaggg tgatccggac gagacgcatg aatgcgggcg gcgcgggcag
       61 cgccttggcg tcgagatcaa tccgcatgag cagctcggaa tcgattttct cggcgtcctg
      121 cgcggaaacc ggttcgaacg atggcgccag gacggctgcg cccaccagtg agagggcggt
      181 ccggcgtgaa aagcgcggca gacgtgcaga catatgaccc ccggaatagc gtggcacggc
      241 ggcacctctc cattgtccgc cgtgggcttg tagagtttcg catatggggc agaatcggga
      301 cgacgttggg gagtgcggat ggacataagg caggtcggag tgattggcga tccggtcgcg
      361 cactcactct cgcccgtgtt ccagcaggca gcgtttgacg cgctgggaat accggcgcgg
      421 tacgagcggt ggcatacgcc tgcccaggaa ctgccggcgc ggataggcag cctgcggagt
      481 cccggcttct tcggcgcgaa cgtcacggtg ccgcacaagg ttgcagttgc cggactggtc
      541 gatacgctgt ccgagacggc gcagcgattg ggcgcggtca acacggtgat caaccggggc
      601 ggccggctgc acggcgacaa taccgatctc tacggcttca tgcgctcgct gctgacggtc
      661 cggccgagcg tcgaacggga tcgcgtgctg atcatcggag caggtggcgc tgcccgggcg
      721 gtggttgctg cggtcgcgga aatcggggtg accggggtca cgatcgccaa ccgctcgcgc
      781 gatcgggccg agcaattgct ggccaatctg cgtatcggcg ccgccgagat tattccgctg
      841 accgattggg acctgatgcg agcgatccgg gaccactcgg tgatcgtcaa cgcgacctcg
      901 atcgggtgga atgacgatca gacggtgctc gcgccggagt tgctcgacgc cgtcgacccg
      961 caggggctga tcgcggacct gacgtatcgc gacacgccgc tgctcgtcca ggcaaaggcg
     1021 cgggggctcc ggacactgga cggactgccg atgctcgtcc accagggcgc gcggtcgttt
     1081 gagctctgga ccggacggga cgcgccaatc gatgtcatga tggcggcggc ggagcgggcc
     1141 agggagttgt aacgggggat gctgatggcg gctgttgtcg ctggtgttgt tggattgttc
     1201 ctggcgagcg tgctctggcg cctggcgcgc cggcaggcat tgcgaaagcc gctctttgcc
     1261 ggccgcgaat ccgaatcgct ggccgcgtgg ctcccgctct gggggctcgg tcagtcgcgg
     1321 attgaggcgg agaccggcga ggagcggcgg ccctggcagg cggtattcga ggttgcgacg
     1381 gcggcctatt tcgctgtgct gggctggcgc ttcggcttct cgatcgatct gctgatggtg
     1441 gtctgtttct ccattccatt gctggtcatc ggactggtcg acttctggac ccggctgatc
     1501 cacaccaacg tgatcttcct cggggtggcg ctcggctggg tcttcgcggt actggatggc
     1561 ggacgcgggc tgctcgattc ggtcattggc atggccgtcg gcggcgcggt cttcatcttc
     1621 ttcttcttgg ccgcgattct gatttaccgc aatatccgcg tcgtgccgtt cgggcttggc
     1681 gatgtctacc tcgccgccat gatcggcgcg atggtacgga tcgaccgcgt catgcaggcg
     1741 ctcctcatcg gcatcttcct ggccgggatc attctggccg cgctgctggc cgcgcgcgtg
     1801 ctgagccgaa agcaagcggt cgcctacggt ccgtacctct gcctcggcgc gctgattacg
     1861 ctgatgctga agtagccacg ccccagcctg agccggcggc cgcgatcaat tgctcgcgag
     1921 cagagcgcag agcctcgatc ggcgtttgcc ggcccaggag ggcagttcgc acctcatcac
     1981 cgatgacccg ggtgacaacg tcg
//
LOCUS       DASSGX010000455         3659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_841890, whole genome shotgun sequence.
ACCESSION   DASSGX010000455 DASSGX010000000
VERSION     DASSGX010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_841890"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1082)
                     /locus_tag="VFI12_08115"
     CDS             complement(<1..1082)
                     /locus_tag="VFI12_08115"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET7056414.1"
                     /translation="MSASNAFGQLSRTMQRWIWDQSWSELRDIQELAVAPILAREDVI
                     VSAATASGKTEAAFLPILTAVEPVARTELAVLCISPLKALINDQEIRIRSMCERIEAE
                     VTPWHGDVAASRKAKLRRNPAGVLLITPESLEAMFVLRGSSVPGMFQNLQFVVVDELH
                     AFIGNERGRQLQSLLNRVELATGNRIPRIGLSATIGDLPLAAQFLRPEEPESVRLIAS
                     DAAYRPVQLQLRGYEIDEPDDPTEAAAQQDPIVRDLYEIHKGATNLIFANAKTNVERY
                     ADALTRRCETDRRPNEFFAHHGNLSKELREDAEERLKSGRPTTAIATSTLELGIDIGA
                     VDAVAQIGVPPTVSSLKQRLGRSGRRG"
     gene            complement(1324..2631)
                     /locus_tag="VFI12_08120"
     CDS             complement(1324..2631)
                     /locus_tag="VFI12_08120"
                     /inference="COORDINATES: protein motif:HMM:NF022376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET7056415.1"
                     /translation="MSDRPIRPRDRDAILQSLSAGVVPRRGLQYIQVGRAGEVKALLQ
                     DLDRVADGGGAIRLIIGEYGSGKTFFEQLIRMAALEKNLVTMHADFNPDRRLHGSHGQ
                     ARSLYAELTRNMATRSSPEGGAVGAVVEKFISATMDAARRDDTNVPAEMRRRMGSLSE
                     LVGGFDFAHVIEAYWRGFQEGNADLQEAAIRWLRGEYSTKTEARQDLGVRTIVDDANV
                     YDQIKLLSRFVVLAGYSGLYVVLDEAVNLYKLTSTQARTSNYEQLLRIVNDALQGSVE
                     HLGFMIAGTPEFLTDTRRGVYSYEALQSRLSENRYLGAGLTDYSGPVIRLASLTPEEL
                     YVLLTRLLAVYGPDEQGHLLLPDDAITAFMRHSSERIGSDYFRTPRTTIRAFVQLLAV
                     LQQNPGTHWTDIVGSIPVEPDQDESALIPDISDGGDELATFRL"
     gene            complement(2624..>3659)
                     /locus_tag="VFI12_08125"
     CDS             complement(2624..>3659)
                     /locus_tag="VFI12_08125"
                     /inference="COORDINATES: protein motif:HMM:NF026952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tellurite resistance TerB C-terminal
                     domain-containing protein"
                     /protein_id="HET7056416.1"
                     /translation="PSVLVAKDSLLSFFPEIPAAPTPAQATGISRLFERIGYGIEPDP
                     ASMRTPFLRAERVGIYRIEFGSGNYYGHRDLSLPLALLSMWSYIAASDGPISERTGTS
                     VCALLVAQADLQPFEINRLHAHAAWLTQHPASLNDARRRYAAADDSNIDGTALTIVAM
                     ALIDAAMTPTRIKALTKVYGVLGSTSERLHANIHRMSTRHVAPPQIIDGEPVAGFGIP
                     AAPDPHRITLDTDRVAAVREATDSIAMVLQGVFTGDETAEHPVSISNPAPAAEDPYLP
                     LLEQLATHRHWAMSELASLAASAGFMTAGVIETLNDRAIALGLEPLLDCDGEGCDCYE
                     PTLKELLAHV"
BASE COUNT          637 a         1052 c         1308 g          662 t
ORIGIN      
        1 ccccggcggc cggagcgccc caaccgttgc ttcagcgatg agacggtggg tgggacgccg
       61 atctgggcga cggcatcgac cgcgccgata tcgatcccga gctcgagggt ggaggtggcg
      121 atggcggtgg tgggacggcc gctcttgagg cgttcttcgg cgtcctcgcg cagctctttc
      181 gagagattgc cgtgatgagc gaagaactcg ttgggacggc ggtcggtctc gcagcgacgg
      241 gtgagcgcgt cggcgtagcg ctcaacgttc gtcttggcat tggcgaagat gaggttggtg
      301 gcgcctttgt ggatctcgta gagatcgcgc acgatcggat cttgctgggc cgcggcttcg
      361 gtcgggtcgt cgggctcgtc gatctcgtag ccgcgtagtt gaagctgcac cggccggtag
      421 gcggcatcgg acgcgatcag ccggaccgat tccggctctt ccggccgcag gaactgggcc
      481 gccagcggga gatcgccgat ggtggcggag aggccgatgc ggggaatgcg gttgccggtc
      541 gccagctcta cgcggttcaa cagcgattgc agctggcggc cgcgttcgtt gccgatgaag
      601 gcatgcagct catcgaccac gacgaattgc aggttctgga acattccggg caccgaggag
      661 ccgcgcaaga cgaacatcgc ttccagtgat tcgggggtga tgagcaagac cccggcggga
      721 ttgcgccgca gcttcgcctt gcgtgaggcg gcgacgtcgc catgccaggg agtgacttcg
      781 gcttcgattc gctcgcacat ggaacggatg cggatttcct gatcgttgat gagcgccttg
      841 agcggcgaga tgcagagtac ggccagctcg gtgcgagcga cgggctcgac cgcggtcagg
      901 atcggcagaa acgcggcttc ggttttgcca gaggcggtgg cggccgagac gatgacatcc
      961 tcgcgcgcca ggatcggtgc gaccgccaac tcctgaatat cgcgtaactc gctccagctc
     1021 tggtcccaga tccagcgctg catcgtccgg ctgagctgcc cgaaggcgtt cgacgcgctc
     1081 atgatccggc cggcgccaaa ctgaccgcaa cgattcgacg accgatgggt agggacggct
     1141 ccctgtggcc gcccttgttg cagggcacgt cagtcgggat gggaaaaccg ttgccgtggc
     1201 gattgacgtt ttggcaactg ggtcgccacc gccgacaggc atgctggcca gaagggcaac
     1261 cccctgtggt tgcccctacc gagtggggac agtatcgagc cgggcggatg agggccatag
     1321 tacttacaac cggaaggtgg cgagctcgtc gccgccgtcg gagatgtcgg ggatgagcgc
     1381 gctctcgtcc tggtcgggtt cgaccgggat actgccgacg atgtcggtcc agtgcgtgcc
     1441 ggggttttgt tgcaaaacgg ccaggagctg gacgaaggcg cggatggtgg tgcgcggggt
     1501 gcgaaagtag tccgagccga tccgttcgga gctgtggcgc atgaaggcag taatggcgtc
     1561 gtcgggaagg agcagatggc cttgttcgtc cggaccgtag accgccagca gccgggtgag
     1621 caaaacgtag agctcttccg gcgtcagact ggcgagccgt ataaccggac cggaatagtc
     1681 ggtcaagccc gcgccgagat agcggttttc cgagagccgc gattgtaagg cctcgtagct
     1741 gtagaccccg cggcgggtat cggtcaggaa ctccggcgtg ccggcgatca tgaagccgag
     1801 atgctcgacg ctgccttgca gggcgtcgtt cacgatgcgg aggagctgtt cgtagttgct
     1861 ggtgcgcgcc tgggtgctgg tgagcttgta gagattgacg gcttcgtcca ggacgacata
     1921 gagcccagaa taaccggcca gcaccacgaa gcgagacagg agcttgatct ggtcgtagac
     1981 attggcgtca tccacgatgg tgcgcacacc cagatcctgc cgcgcttcgg tcttggtcga
     2041 atattcgccg cgcagccagc ggatggcggc ttcctggaga tcggcgtttc cttcctggaa
     2101 gccgcgccaa taggcttcga tgacatgggc gaaatcgaag ccgccaacca gctcggagag
     2161 gcttcccatg cggcggcgca tctcggccgg gacattcgtg tcatcccggc gggcggcgtc
     2221 catggttgcg gaaatgaact tttcgacgac cgcgccgacg gcgccaccct cggggctgga
     2281 gcgcgtggcc atattgcggg ttagctcggc gtagagactg cgcgcttgcc cgtgcgagcc
     2341 gtgcaagcgg cggtcgggat tgaaatcggc gtgcatggtg acgaggttct tttcaagcgc
     2401 cgccatacgg atgagctgtt cgaagaaggt cttgccgctg ccatattcgc cgatgatgag
     2461 ccggatggcg ccgccgccgt cggccacccg gtcgaggtcc tgcaacaagg ctttgacctc
     2521 tccggcccgc ccgacctgga tgtattgcaa gccgcggcgg ggcacgacgc ctgccgatag
     2581 cgactgcagg atggcgtccc ggtcgcgggg ccggatcggg cgatcagaca tgggcgagta
     2641 gctccttcaa tgtaggctcg taacaatcgc agccctcgcc atcgcagtcg agcagcggct
     2701 ccagcccgag cgcaatcgcg cgatcgttga gcgtttcgat cacgccggcc gtcatgaaac
     2761 cagccgaggc ggcgagcgaa gccagctcgc tcatcgccca gtgccggtgc gtagcaagct
     2821 gctccagtaa gggcaagtag ggatcttcgg cggcgggcgc cggattggaa atcgagaccg
     2881 ggtgctcggc tgtttcgtct ccggtgaaga ccccctgcag caccatggcg atcgagtcgg
     2941 tcgcttcccg cacggccgcg acgcggtcgg tgtcgagcgt gatccgatgc ggatcgggcg
     3001 ccgccggaat gccgaatccg gcgaccggct caccgtcgat gatctgcgga ggagcgacat
     3061 ggcgcgtcga catgcggtgg atgttggcat gtagtcgttc gctggtcgag ccgaggacgc
     3121 catagacctt ggtgagcgct ttgatgcggg tgggtgtcat ggcggcatcg atcagcgcca
     3181 tggccacgat ggtgagcgcg gttccgtcga tgttcgagtc atcggctgct gcgtagcggc
     3241 gtctggcatc gttcaatgac gctggatgct gggtcagcca ggcggcgtgg gcatgcaggc
     3301 ggttgatctc gaagggttgc agatcggcct gggcaaccag gagcgcgcag accgacgtac
     3361 cggttcgttc cgaaatcggc ccatccgatg cggcgatata ggaccacatg ctgagcaacg
     3421 ccagcggaag ggaaagatcg cggtggccgt agtagttccc tgagccaaat tcgattcggt
     3481 agatgccgac gcgctcggcc cggagaaacg gggtgcgcat cgatgcggga tcgggttcga
     3541 tcccataacc aatccgctcg aacaatcggg agatgccggt tgcctgcgcc ggggttggcg
     3601 cggcggggat ctctggaaag aaggacagca gcgagtcttt cgcgacaagc acggatggg
//
LOCUS       DASSGX010000456         2461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_186965, whole genome shotgun sequence.
ACCESSION   DASSGX010000456 DASSGX010000000
VERSION     DASSGX010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186965"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            395..1945
                     /gene="metG"
                     /locus_tag="VFI12_08130"
     CDS             395..1945
                     /gene="metG"
                     /locus_tag="VFI12_08130"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF020892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HET7056417.1"
                     /translation="MSRGAPFYITTAIDYPNGEPHVGHSFEKISADIVARYHRLLGDD
                     VAFCMGLDENSQHIVTAAEKAGMETRAFADLMEENFLRAWNALEISVNAWTRTTEPRH
                     ARASIELFDRAVANGDIYKATYAGWYCPNCNNYYTDAELVNGRCPLHPTITPEWLEEE
                     NYFFALSKYTDVLKRHLEEHPEFVTPAVWRSEMMALIDRGLRDFSVSRQVKPGKPAWG
                     IPVPGDPDHVMYVWYDALTTYLTAIGFPDDAERFNRYWPADSQIIGKDITRFHILYWP
                     AMLLSAGLELPRRIVVHGFMTLEGQRISKTTGNTVSPIELVEEFGADPIRYYIARDMT
                     FTSDGDFSRANLIRRYNNDLGNDLGNLLNRVVSMIGRYRGGDVPSADEAGDLERDVQA
                     VAANAVERAGQLIENWELDDALDAIWVLIRRANQYLEERKPWTLAKSPDNAGALDTTL
                     WTAAEATRIAGILLAPYIPATANRIMDQLGLATVADGDWGTRTAWGSVTFGTVNPQGP
                     LFPRIETE"
     gene            2023..>2461
                     /locus_tag="VFI12_08135"
     CDS             2023..>2461
                     /locus_tag="VFI12_08135"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HET7056418.1"
                     /translation="MACTLPAGSRLLLTLAIAVSTLMAPATVWHAPAALAATDQCLSA
                     NEALLLDRINTFRQVNGLAPIIASPTLTAAARHHAGSMATYNYFPADYSVRHEGPDHD
                     QTITWQQNIANAGYPDNTHTTRSAIIGAGTDAVAAIYRSLTDLP"
BASE COUNT          466 a          803 c          784 g          408 t
ORIGIN      
        1 tacacctgcg ggacacgtag gccttgaagg acctgacgat cgaaatgagg tcgattgagc
       61 cgatctgaaa tacggcgtga aggtggtccg gcatcacaga gtagaggagc aaatcggcgt
      121 cccggaacgc gctttgccga acgagttcct cggtaaccac tcgtgcgacc ctcgggtctg
      181 ccatgaccgg tcggcgacgt tccgcgtcga tcgtcacgtg ccagacagag ccgggaactc
      241 catatgcctc caggggcagg cgaatctgtt ttcgtggcgg gtatcgcatg gccgggatta
      301 tgggcgaaag ttcaggggcg cgtgctaatc tgattggtgg ttcgagccgc ggggaggcgc
      361 ccgcggttct catttctccg gaaggttgac attcgtgtcc aggggcgccc cgttctacat
      421 cactacggct attgattacc cgaacggcga gccgcacgtc ggccattcgt tcgagaagat
      481 ctcggccgat atcgtggccc gataccaccg gctgctcggg gacgacgtcg ccttctgcat
      541 ggggttggac gagaacagcc agcacatcgt cacggcggca gagaaggcgg ggatggagac
      601 gcgcgccttc gccgacctga tggaggagaa cttcctccgc gcctggaatg cgcttgagat
      661 ctcagtcaac gcctggaccc gaaccaccga gccgcgtcac gccagggcct cgattgagct
      721 attcgaccgg gcggtggcga atggcgacat ctacaaggcg acctacgccg gctggtactg
      781 cccgaactgc aacaactact acaccgacgc cgagctggtg aacgggcgct gcccgctgca
      841 cccgacgatc acgccggaat ggctggagga ggagaactac ttcttcgccc tcagcaagta
      901 cacggacgtg ctcaagcggc acctcgagga gcatcccgag ttcgtcacgc cggcggtctg
      961 gcgatcggag atgatggcgc tgattgaccg cggcttgcgc gacttctcgg tctcgcgcca
     1021 ggtcaaaccg ggcaagccgg cctggggcat ccccgtgccg ggcgacccgg accacgtcat
     1081 gtacgtctgg tacgacgcgc tgacgaccta cctcaccgcg atcggcttcc cggacgacgc
     1141 ggagcggttc aaccgttact ggccggcgga ttcgcagatc atcggcaagg acatcacccg
     1201 cttccatatc ctctattggc cggcaatgct gctctcggcc ggcctggagc tgccgcgccg
     1261 gatcgtcgtg cacggcttca tgacgctcga aggacagcgg atctcgaaga cgaccggtaa
     1321 taccgtctcg ccgatcgagc tggtcgagga gttcggggct gatccaatcc gctactacat
     1381 tgcgcgggat atgaccttca cgtcggatgg cgacttctcg cgcgcgaacc tgatccgccg
     1441 gtacaacaac gacctgggca acgacctcgg caacctgctc aaccgcgtcg tcagcatgat
     1501 cgggcggtat cgcggcggag acgtgccgtc cgccgacgaa gcaggcgacc tcgagcggga
     1561 cgtgcaggcg gtagcagcga acgccgtgga acgggccggg cagctgatcg agaactggga
     1621 gctggacgac gcgcttgacg cgatctgggt tctgatccgc cgcgcgaacc agtacctcga
     1681 agagcgcaag ccgtggacgc tggccaagtc gcccgacaac gccggcgcgc tcgataccac
     1741 gctctggacc gccgccgagg cgacccggat cgccggcatc ctgcttgcgc catacattcc
     1801 cgcgaccgcg aatcggatca tggaccagct cggactggcg acggtcgcgg atggagactg
     1861 gggcacccgc acggcgtggg gatcggtcac tttcggcacg gtgaatccgc aaggtccgct
     1921 cttcccgcgc atcgagaccg agtagcacgc ggattgacat cggccggtca ggtgctgatg
     1981 atgggtgcgc aatcccgtcg cccatgcgct ggagcacagc caatggcatg caccctgccc
     2041 gcaggatcaa gactcctgct cacgctcgcc atcgccgttt cgaccctcat ggctccggca
     2101 acggtgtggc atgctcctgc tgccctggcc gccacggacc agtgcctcag cgcgaacgag
     2161 gcattgctgc ttgaccggat caacaccttc cggcaggtga acgggctcgc gccgattatc
     2221 gccagcccga ccctgaccgc cgccgcgcgc caccacgccg ggagcatggc gacgtacaac
     2281 tactttccgg ccgactactc ggtacggcat gagggtccgg atcacgacca gacaatcacc
     2341 tggcagcaga acatcgcgaa tgccggctat cccgacaaca cgcacacgac ccggagcgcg
     2401 atcatcggcg ccggcacgga tgcggtcgcg gcgatctatc ggtcactcac ggacttgccg
     2461 g
//
LOCUS       DASSGX010000457         2598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_295977, whole genome shotgun sequence.
ACCESSION   DASSGX010000457 DASSGX010000000
VERSION     DASSGX010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295977"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VFI12_08140"
     CDS             complement(<1..306)
                     /locus_tag="VFI12_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056419.1"
                     /translation="MDDEHFDAWTRRQVGVAAGGTLAALLRLLHADEAEAKHHHHNRR
                     QKRRRRQKRRERRRHHDHTPPCSGPDCGGDHPDIILINVDDMREADFIALPQTQALLG
                     "
     gene            494..851
                     /gene="ssrA"
                     /locus_tag="VFI12_08145"
     tmRNA           494..851
                     /gene="ssrA"
                     /locus_tag="VFI12_08145"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1031..1474)
                     /locus_tag="VFI12_08150"
     CDS             complement(1031..1474)
                     /locus_tag="VFI12_08150"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET7056420.1"
                     /translation="MAVTVTHVPGTTYQTRIDNGDLAFLADEPVASGGDGLGPGPYDL
                     LLAALGHCTVLTILLYARRKGWPVEGVSVRASHERSTVEAEVDGVPIRRKVERITQEI
                     ALDGPLDEAQRTRLIEIAGRCPVHRTLVGNLEIVEVEAAPTAVER"
     gene            complement(1524..1817)
                     /locus_tag="VFI12_08155"
     CDS             complement(1524..1817)
                     /locus_tag="VFI12_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HET7056421.1"
                     /translation="MHLCFGPNCTQRGSRALLPVLERAVREAGLGGCVEVLATTCRNR
                     CDYGPSLNVYPGPVFYNGVDAAAIVDIVRTHLAGGAVVERWLYRPSLAAGKRR"
     gene            complement(1858..2349)
                     /locus_tag="VFI12_08160"
     CDS             complement(1858..2349)
                     /locus_tag="VFI12_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HET7056422.1"
                     /translation="MTENTSPAYWIVVGSPENFQRTAEHGFTVQGLKSRHRKKAERMK
                     PGDKIVYYLTGRKAFGGVASIESPYFESHEPIWQSSDPKKAAEDYPFRVRISPDLILP
                     LDEAVPAEGIARQMAYVAKWPANNWTLAFQGNVHEIGEDDYRLIREAIDGAVPVGAGV
                     VPG"
BASE COUNT          420 a          903 c          836 g          439 t
ORIGIN      
        1 gccgagcagc gcctgcgtct gcggcagggc gatgaagtcg gcctcccgca tatcatcgac
       61 attgatcagg ataatgtcgg ggtggtcgcc gccgcagtcc ggacccgagc acggcggggt
      121 gtggtcatgg tgacggcggc gctcccgccg tttctggcga cgccggcgct tctggcgccg
      181 gttgtggtga tggtgtttcg cctcggcctc atccgcgtgc agcaggcgca gcagcgccgc
      241 cagagtgccg ccggcggcca cgccgacctg tcgccgcgtc caggcgtcga agtgctcgtc
      301 gtccatggcg tgctcctgag gagaaggtgg ggcggttcag ccgcggcgag tggcgagatg
      361 gcttggccac cctgagccac gaggaccgga gcgggtcaat ccgacccgga gggggaactc
      421 tacacggcca tgcatacgct gacaacacga ccagtggtcg cgagcggcac attatggtat
      481 gattcccggg cttggggatg tttggtttcg acagggtcgt aggtcaggtc gatgcgtgcc
      541 gaggcgcccg aggcctcgta aaaacgggca acccaagaaa tgcggataat ttcgcactcg
      601 ctgcctaagt cactgacctc taggtagtgc gtccgtccgg acctccccct tccgggaccg
      661 ggtacgggcg tcacccagga agggtgccgc gatcaagtct gccgaagcgc gatcgctcaa
      721 gccagttcgg ctcactgcca ccgcatcccg tcggtaggag acggcggcag cacacaaaat
      781 gccgactacg cacgtaggaa gcggccggct cgcggttctg gacgggggtt cgactccccc
      841 catctccacc agcagcacag cgcccctgga tcaccagggg cgctttcgtt tgcgccgaga
      901 tgcctggtaa gagcaagacg cacaaatcgt gtcattctga ggcgaagccg aagaatctcc
      961 ggcgcgacgg tgacggcgaa cgagattctt cgcctgcggg ctcagaatga cacgatttgt
     1021 gcgcgatact ctaccgctcg acggcagtcg gggcggcctc gacctccacg atctccagat
     1081 tgccgacgag cgtgcgatgc accgggcacc gccccgcgat ctcgatcagc cgcgtccgct
     1141 gcgcctcatc cagcggcccg tcgagcgcaa tctcctgcgt gatgcgctcc accttgcgcc
     1201 ggatcggcac gccgtcgacc tcggcctcga ccgtcgatcg ctcgtgactg gcgcgcaccg
     1261 agaccccctc caccggccag cccttgcgcc gcgcgtagag caggatggtg agcacggtgc
     1321 agtgccccag cgcggccagc aacaggtcgt acgggcccgg ccccagacca tcgccgcctg
     1381 atgccaccgg ctcgtcggcc aggaaggcca gatcgccgtt gtcgatccgc gtctggtacg
     1441 tcgtccccgg cacgtgcgtg accgtcaccg ccacctgccg gctcctctct gatcacgcgt
     1501 cccgtggtag cgattgccgc cgctcatcgc cgctttcccg ccgccagact cggtcggtag
     1561 agccagcgct cgacgaccgc gcccccggcc agatgcgtcc gcacgatatc cacgatcgcc
     1621 gcggcgtcga cgccgttgta gaagaccggt cccggataga cattgagcga cggcccgtaa
     1681 tcgcagcgat tgcggcaggt cgtcgccagc acctcgacgc acccgcccag tcccgcctcg
     1741 cgcaccgcgc gctccagcac cggcagcagc gcccggctgc cgcgctgggt gcagttcggt
     1801 ccgaagcaga gatgcacccg gtacgagcaa cccgcggcgc aacgcgaatc gcctgggcta
     1861 tccggggaca accccggctc ccaccggcac cgcgccgtcg atggcctccc ggatcagacg
     1921 gtagtcgtcc tcgccgatct cgtgcacatt gccctggaac gccagcgtcc agttgttcgc
     1981 cggccacttg gcgacgtacg ccatctgccg cgcgatcccc tccgccggca cggcctcgtc
     2041 cagcggcagg atcagatcgg gcgagatgcg gacgcggaac gggtagtcct cggccgcctt
     2101 cttcgggtcg ctgctctgcc agatcggctc gtggctctcg aaatacggcg attcgattga
     2161 tgccacaccg ccgaacgcct tgcggccggt caggtagtaa acgatcttgt cgcccggctt
     2221 catccgctcc gccttcttgc ggtgccggga tttgagcccc tgcaccgtga agccatgctc
     2281 ggcggtgcgt tggaagttct ccggcgaccc gaccacgatc cagtaggcgg gcgaggtgtt
     2341 ctcagtcatg gttctgcgcg tcctttcgct ccatgcgggg cgattggttc gagtatgtca
     2401 cgcgcggtca accggcggtg tactccttgt cgcgccggga tggtggcaac caatacgcca
     2461 gccccgtcca ggaggcttct tgcgggaggc agccaaacgt cgttaccgca acgcgcgaga
     2521 accgtgtcat gctgagtacc gcgtccacgc actcgtgtca tcctgagcga agcgaaggat
     2581 ctcgcctgct tgcaggcg
//
LOCUS       DASSGX010000458         4407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_79557, whole genome shotgun sequence.
ACCESSION   DASSGX010000458 DASSGX010000000
VERSION     DASSGX010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4407
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79557"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..93
                     /gene="yidD"
                     /locus_tag="VFI12_08165"
     CDS             13..93
                     /gene="yidD"
                     /locus_tag="VFI12_08165"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HET7056423.1"
                     /translation="MKGGWMATKRVARCNPFAKGGHDPVP"
     gene            738..1886
                     /locus_tag="VFI12_08170"
     CDS             738..1886
                     /locus_tag="VFI12_08170"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HET7056424.1"
                     /translation="MPFDTLHLPLAIPVWAQYVDFLKWVLSRIGDAVGSGGVAIIIFT
                     IIVRTLILPITIRSIKSMKSMQDIQPKIKELQKKHKGDRMKIQQETMALYQTYGVNPV
                     AGCLPALLQLPIFFGVYRAIYGLSKSDTGVWHGDFLWLHSLKAPDPWHLLPILAGIFQ
                     LMQTFMSRPHNQGKVTDPQQAMMNTMMNIMPITVVLFGWSFASGAVIYWVTQSLYGII
                     QQWFITGWGKLNDYIPNLPELPEHKRLGYRPPRDLEELANQPPKKKGVFGRWWEQQMQ
                     HAQQMQEERKTTPAGSAAGGGTAAATAGGLAANAKRSGSGPKAPRPYARNSPKGRMLA
                     EQARRAEEDADDVIDIEADIVADDVRDADISANGTGKTPPKRTRRAKK"
     gene            1977..2822
                     /gene="jag"
                     /locus_tag="VFI12_08175"
     CDS             1977..2822
                     /gene="jag"
                     /locus_tag="VFI12_08175"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HET7056425.1"
                     /translation="MRDQERLTSVEIQAVSVEDAIRLALDQLQLGREDVDIEILSDAG
                     SSEDEEALVRVTAKGMASQPVSSSAGSRSTVGGARPQYRDRNGQRRAPGPRRVPGDRP
                     SSRGAPPARVERNVDPNRVDAEDEAQVKEIVSDLLERMGLDADVMAIDNPSILPMTEE
                     DAPTIFIDVVGSDPGMLIGRRGEHLAQFQYLVNLICSKQMKDARRVVIDVEGYRTRRE
                     ESLIGLAERVARQVSRSRRPMMMEPMPANERRIIHLKLREHPDVYTESSGEGSQRRVE
                     VRPRD"
     gene            2815..3804
                     /locus_tag="VFI12_08180"
     CDS             2815..3804
                     /locus_tag="VFI12_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET7056426.1"
                     /translation="MTDRPARPSTDGTSEAKRSDSGTARSKPRASGRPGQSQRPPDYV
                     VIGHISADILPDGKAVLGGTALYSALAAATFGWRVGVLTRGKYGFNLNGLEIPPLEFG
                     HERIQVVVQDAEWPTCFVNEYWAAGRRTQQIPRWAGPIDLRGLPPSWQSARVIHLGPI
                     AQEIDVRQSAGLNPRFLGATPQGWMRDWPRGPGGRVQPIHLRLPGEFVSQLDGIVVND
                     EEFSYSRDTVEAVGKDGYGVVTFGENGARLYSPRGQTTVPSFKVPVVDLTGAGDVFTA
                     AFFYRITDPSFTPEEALQFAHAAAGLSLGGVAATHIPTRPEIKRLMDRVADSV"
     gene            3809..>4407
                     /locus_tag="VFI12_08185"
     CDS             3809..>4407
                     /locus_tag="VFI12_08185"
                     /inference="COORDINATES: protein motif:HMM:NF021882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HET7056427.1"
                     /translation="MPIDFGTIAAVATAPGEAGIAVIRVSGPDVREIAERVIRVGRTG
                     PALILAPDNSHQLFYGRVIDPTSDHALDEVMVAWMAGPRSFTTEDTIEISCHGGAVAV
                     QQVLRAVLAAGARPANPGEFTMRAFLNGRLDLTEAEAVLRVVSAQTTEGLERALDDLR
                     GDLTRRLEPARAAVLAALAYLDASADFPDDEIPVSNVHA"
BASE COUNT          758 a         1442 c         1485 g          722 t
ORIGIN      
        1 cggtacgggg cgatgaaggg cggctggatg gcgacgaagc gggtggcgcg ctgcaacccg
       61 ttcgcgaagg gcgggcatga tccggtgccg tgacggacga gaaccgggcc ttgtgctgac
      121 aacatggcgt ccgtctccgc ccggggataa atccccgggc tacatcacat aagccagcta
      181 aatcaggctc gattttgggt cggttttaac cgacatccgt cccctaatcc cggatttcaa
      241 tccggggcgg gcatgacgtc gcggtcatcg aagagcgggc ccccgggcag cacagccggg
      301 tgaggccgcc cggggatacg ttgggcagcg cgcgcctgtc tcagcccctt cagtgggctt
      361 ttcattcctc agcccgggca tttatcctcg ggcggactcg aacggccggt tcgatattcg
      421 ggacccgctc ttcccgcacg cactttcgtg gcctgcggcc accgccgggc acatcccctc
      481 tccaactctc cccttcaggg gagagaacat cgccggtgcc acgcttgcag gcgccggcca
      541 tagatcggcc aattcttttg ttgggacaat ttccccctct ggaaggtggc cgggagcggt
      601 tatgttccgt agacttgcgc aggcagactc gcagcctgcc cttcacatga tctgtatccg
      661 gacctggctg gttcactgac tccccgtggc caggtcatct gaaagcgtcc cgctctcggc
      721 gggggaggct tacaccgttg ccgttcgata ccttgcacct tccgctcgcc atccctgtct
      781 gggcgcagta cgtcgacttc ctgaagtggg tcctctcaag gatcggcgac gcggtcggca
      841 gtggcggcgt cgcgatcatc atcttcacga tcatcgtccg caccctgatc ttgccgatca
      901 cgatccgctc catcaaatcg atgaagtcga tgcaggacat ccagcccaag atcaaggagc
      961 ttcagaagaa gcacaagggc gaccggatga agatccagca ggagacgatg gcgctgtacc
     1021 agacgtacgg tgtcaatccg gttgccgggt gtctgccggc gctcttgcag ctgccgatct
     1081 tcttcggcgt ctaccgggcg atctacgggc tttcgaagag cgacacaggc gtttggcacg
     1141 gcgattttct ctggctgcac agcctgaagg cgccggaccc atggcacctg ctgccgatct
     1201 tggccggcat cttccagctc atgcagacct tcatgtcccg cccgcacaac cagggcaagg
     1261 tgaccgaccc gcagcaggcg atgatgaaca cgatgatgaa catcatgccg atcacggtcg
     1321 tgctgttcgg ctggtcgttt gcgtcgggcg cggtcatcta ctgggtgacg cagagtctct
     1381 acggcatcat tcagcagtgg ttcatcaccg gctggggcaa actcaacgac tacatcccga
     1441 acctgccgga gctgccggag cacaagcgac ttggctaccg tccgccgcgg gatcttgagg
     1501 agctggcgaa ccagccaccc aagaagaagg gcgtgttcgg ccgctggtgg gagcagcaga
     1561 tgcagcatgc ccagcagatg caggaggagc gcaagacgac tccggccgga agcgcggcgg
     1621 gcggcggcac tgccgcggca accgcagggg gcctggccgc gaatgccaag cgctccggat
     1681 cgggaccgaa ggcgccccgc ccatacgcgc gcaactcgcc gaaaggccgg atgctggccg
     1741 agcaggcgcg gcgcgctgag gaagacgcgg acgacgtgat cgacatcgag gctgatatcg
     1801 tggcggatga cgtgcgggat gccgacatct cggcgaacgg gaccggcaag acgccaccga
     1861 agcgaacgcg gcgcgccaag aagtaacgaa tcgccaattc aaacggcaaa cgcggtacta
     1921 tccagggcag atcgatgatg atcggcccca tgatcgggag ataccagata cgccctatga
     1981 gagaccaaga gcggcttacc agtgttgaga ttcaggcagt gtcagtcgaa gacgccattc
     2041 ggctcgcgct cgatcagctt caactcggtc gggaggatgt cgatatcgag atcctgtccg
     2101 atgcgggctc gtcggaagat gaggaagcgc tggtccgcgt cactgccaag ggcatggcct
     2161 ctcagcccgt ctcatcgagc gccgggagcc ggtccactgt gggcggcgcg cgaccgcaat
     2221 accgcgaccg caacgggcag cgccgcgcgc ccggtccgcg ccgggtgccg ggtgaccgac
     2281 cgtccagccg gggcgcgccg ccggcgcgtg tcgagcggaa cgtcgatccg aaccgggtcg
     2341 atgccgagga tgaggcccag gtcaaggaga tcgtgtcgga tctccttgaa cggatgggac
     2401 tcgatgctga cgtgatggcg atcgacaatc cgtcgatcct gccgatgacc gaagaggacg
     2461 cgccgacgat cttcatcgac gtggtcggca gcgacccggg tatgctgatc ggccgccggg
     2521 gcgagcacct ggcccaattc cagtacctgg tcaacctgat ttgctcgaaa cagatgaagg
     2581 atgcgcggcg cgtcgtgatc gacgttgagg gctaccgcac gcggcgggag gagtcgctga
     2641 tcggcctggc cgagcgcgtc gcccgccagg tgtcccgcag ccgccgcccg atgatgatgg
     2701 agccgatgcc ggccaatgag cgccggatca tccacctcaa gctgcgcgag cacccggatg
     2761 tctacaccga gagctcgggc gagggctcgc agcgccgcgt ggaggttcgc ccgcgtgact
     2821 gatcgccccg cgcgcccgtc caccgacggc acgtcggagg cgaaacggag cgactccgga
     2881 acggcccggt cgaagccacg cgcatcggga cgcccgggcc agtcccaacg accgcccgat
     2941 tacgtcgtga tcggccacat ctcggccgac atcctgccgg acggcaaggc tgtcctcggc
     3001 ggcacggcgc tctactcggc gctggccgcc gccacgttcg gctggcgcgt tggcgtcctg
     3061 acgcggggca aatacggctt caacctcaac gggctcgaga ttccgccgct cgagttcggc
     3121 catgagcgga tccaggtcgt cgtgcaggat gccgagtggc cgacctgctt cgtcaacgag
     3181 tactgggccg ccgggcggcg cacgcagcag attccgcgct gggccgggcc gatcgacctt
     3241 cgtggcctgc cgccatcgtg gcagagcgcg cgggtgatcc accttgggcc gatcgcgcag
     3301 gagatcgatg ttcgccaatc ggccggcctg aacccgcgct tcctcggcgc cacgccgcag
     3361 ggctggatgc gggactggcc gcgggggcct ggtgggcggg tgcagccgat ccatttgcgg
     3421 ctgccgggcg agttcgtcag ccagctcgat ggcatcgtgg tcaacgacga ggagttctca
     3481 tactcgcgcg acaccgtcga ggccgtcggg aaggacggct acggcgtggt tacgttcggg
     3541 gagaacggcg cgcgccttta ctcgccgcgc ggacagacca ccgtgccatc gttcaaggtc
     3601 ccggtggtcg atttgaccgg cgcgggcgac gtcttcacgg ccgccttctt ctaccgcatc
     3661 accgatccgt cgttcacgcc cgaggaggcg ctccagttcg cccacgcggc ggccggcctt
     3721 tcgctcggcg gcgtcgcggc cacccacatc ccgacccggc cagagatcaa gcgcctgatg
     3781 gatcgggtcg ccgacagcgt ctaacgccat gcccatcgac ttcggaacga tcgccgccgt
     3841 cgccaccgcg ccgggcgagg cgggcatcgc cgtgatccgg gtcagcggcc cggatgtgcg
     3901 cgagatcgcc gaacgcgtga ttcgggtcgg gcggaccggt ccggcgctga tcctggctcc
     3961 cgacaactcg caccaactct tctacggccg cgtgatcgac ccgacgtcgg accacgcgct
     4021 cgatgaggtg atggtggcgt ggatggccgg gccgcgttcc ttcacgaccg aggacacgat
     4081 cgaaatctcg tgtcatggcg gagcagtcgc ggtccagcag gtgctgcggg cagtgctggc
     4141 ggccggggcg cggccggcga accccggcga gttcacgatg cgcgccttcc tgaatggccg
     4201 gctcgatctg accgaggccg aggcggtgct gcgcgtcgtt tcggcgcaga ccacggaggg
     4261 gctggagcga gcgctcgatg atctgcgcgg cgacctgacc cggcggctgg agccggcccg
     4321 ggcggcggtc ctggctgcgc tggcctacct cgatgcctca gccgacttcc ccgacgatga
     4381 gattccggtc agcaatgtgc atgccga
//
LOCUS       DASSGX010000459         3266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1497121, whole genome shotgun sequence.
ACCESSION   DASSGX010000459 DASSGX010000000
VERSION     DASSGX010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3266
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1497121"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VFI12_08190"
     CDS             <1..579
                     /locus_tag="VFI12_08190"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HET7056428.1"
                     /translation="LEGGIGVVLIAGTGSIALGRDANGNTARAAGWGHLMGDEGSGYD
                     IGRRGLMAAARAADGRGPETGLLEALMRHFEVDRPLDMIAPAYRSTEKAAIASLASIV
                     FECAQVGDEVAAAIITAAASELAHAAIAAGSQLGLDNEPMPLALAGGLMIGHPSFRNE
                     VVRVIRAARPVGQVSVVDDPALRAAQGLVGAG"
     gene            584..1723
                     /gene="nagA"
                     /locus_tag="VFI12_08195"
     CDS             584..1723
                     /gene="nagA"
                     /locus_tag="VFI12_08195"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HET7056429.1"
                     /translation="MTSITVSGRLLLGSELVPGSVRLAGNEIVDVQRTSGEIPDADLT
                     ADIVAPGLIDLQVNGGHGVEVGADPAAIQHLATWLPSTGVTAFLPTLITSPASVYPDV
                     FSAIRAARSTGGACPLGLHLEGPFLSPLRHGAHRVDLIEAADNALFDLMLAAPEVRLV
                     TLAPERAGAVDRIRRLRERGITVSLGHTDATYEQLVAGIDAGATMVTHLFNAMSPFAH
                     RAPGVIGAALVDDRVAVGLIPDGIHSHPASVQLAVRAKGPERIALVSDMMPAAGMPPG
                     QYQFGGQTVTVDSVSARLADGTLAGSIVTMDQAIRNMVRWTDATPAEAIRMASEIPAR
                     LIGRPDLGTMQPGNSADLTLFHDNLTVQATIIQGCIAFDSRDRRG"
     gene            2391..2618
                     /locus_tag="VFI12_08200"
     CDS             2391..2618
                     /locus_tag="VFI12_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056430.1"
                     /translation="MGFGFCEGDQGDGEHEDTEEGVGQVDAVLVPQLDEGWALLEAGE
                     VREQQETDQDDLKDRCQPEAVLHRIDALHVT"
     gene            complement(2615..>3266)
                     /locus_tag="VFI12_08205"
     CDS             complement(2615..>3266)
                     /locus_tag="VFI12_08205"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HET7056431.1"
                     /translation="ELARAHGARFFQASTSEVYGDPQVHPQTEDYWGHVNPNGPRACY
                     DEGKRFAESLIMEYHRQHGLDVRIVRIFNTYGPRSHPSDGRVVPNFCVQALRGEPITV
                     FGSGQQTRSFQYVNDLIRGFVLFMEGEGLAGEVVNMGNPVEFTMLQFAEEILKITGST
                     SELVFKPLPQDDPTRRRPDIGKAKRLLDWEPVVSLAEGLVPTLAFFEQVLAESAAR"
BASE COUNT          527 a          999 c         1157 g          583 t
ORIGIN      
        1 ctcgagggcg gcattggggt agtgctcatc gccggcaccg gttcgatcgc cctcggtcgc
       61 gatgcgaacg gcaacaccgc tcgtgccgcg ggttggggcc atctgatggg cgacgaggga
      121 agtggctacg acatcggccg ccgaggtctc atggcggcgg cccgggcggc ggatggacgc
      181 ggcccggaga ctgggttgct tgaagcgctg atgcgccact tcgaggtcga ccggccgctc
      241 gatatgatcg cgcccgcata tcgctcgacc gaaaaggccg cgatcgcctc tctcgcgtcg
      301 atcgtattcg agtgtgcgca ggtcggggat gaggtcgcgg cggcgatcat cacggccgcc
      361 gcaagcgagc ttgcgcacgc tgccattgcc gccggatcgc aacttggatt ggacaatgaa
      421 ccgatgcccc tggcgcttgc tggtggcctg atgatcgggc acccgtcgtt tcggaatgag
      481 gtggtacggg ttatccgggc tgcgcggccg gtggggcagg tcagtgtcgt cgacgatccc
      541 gcattacggg cggcgcaggg cctggttggt gcgggctgat acgatgacca gcatcaccgt
      601 ctccggacgc ctcctgctcg gcagcgaact cgtcccgggg tcagtccgcc ttgccggaaa
      661 tgagatcgtc gacgttcagc gcacctccgg cgaaatcccg gacgctgact tgactgccga
      721 tatcgtcgcg ccgggcctga tcgacctcca ggtcaatggc ggacatgggg tcgaagtcgg
      781 cgccgaccca gccgcgattc agcacctggc cacgtggctg ccatcgaccg gggtgacggc
      841 ctttcttccc acgctgatca catcaccggc cagcgtgtat cccgacgtgt tctcggcgat
      901 cagggcagct aggtcgactg gtggcgcgtg tccacttggc ctgcacctgg aaggaccgtt
      961 tctttccccg ctccggcatg gcgcgcaccg ggtcgatctg atcgaggccg cggacaacgc
     1021 cctcttcgac ctgatgcttg cggcgccaga ggtacgactc gtcacccttg cccccgagcg
     1081 agcgggggcc gtcgaccgga ttcgccgctt gcgcgaacga ggaatcacgg tcagcctcgg
     1141 ccacacggac gcgacctatg agcagctcgt cgcgggcatc gatgcgggcg cgacgatggt
     1201 gacgcacctc ttcaacgcga tgtcgccgtt tgcccaccgc gcgcccggcg tgatcggcgc
     1261 ggcgctggtg gatgaccggg tggcggtcgg cctgataccg gacggtatcc acagccatcc
     1321 cgcgagcgtc cagcttgccg tgcgagccaa gggaccagaa cggatcgcgc tcgtgtcgga
     1381 catgatgccg gccgccggca tgccgcccgg gcagtaccaa ttcggcgggc agaccgtcac
     1441 cgttgacagc gtctcggcca ggctcgcgga cggtacgtta gccggctcga tcgtcacgat
     1501 ggatcaggca atccgcaaca tggtccgctg gacagacgcg acgccggcag aggcaatccg
     1561 aatggcatcg gagatccccg cccgcctcat cggccggcca gacctgggca cgatgcaacc
     1621 cggcaactcg gccgacctga ccctcttcca cgacaaccta accgtccagg caacgatcat
     1681 ccaaggctgc atcgcatttg attcgcgcga tcggcgaggc tagagcggtc ggcagaggtt
     1741 gtgccaatct ggcgcactgg ggggcatgca gcgcccatac gcctctgacg atgtccgcga
     1801 acgggccctg gccgcggtgg atgcggggca tccagtggct gacgtcgccg cgttcttcca
     1861 gaacgatcca tcgacgatct cccgctggct gcgccaatgc cgccgggccg gctctggcct
     1921 gtcgcgcccg cgctcgggtc gcccgccgct gctgcggccg gagcaacgcc ccgtcatgcg
     1981 cgcgcaggtg gcaacccatc ccggtgcgac atccctgttc aggggccggg attttgggcg
     2041 cacggggtag ctgtctttcg acggttttcg gttttgggtt ttcggttttc gggacgaaac
     2101 ccaaaacggg caggtggcat ctgcccctac agaaagcgat cgaattgttc tggacgtcat
     2161 cagggcccga atctgtgccg agtcgaactg gggctaggag gatgctctgg cgtatcggtc
     2221 aggaggatca gggaggctca cctgggatta ccggacctgt ctccggcggt tttctcgacg
     2281 cgtcaccacc gcgggaagag ttgtgcactg cccaggcccg tcccccgtcc aaggatgccg
     2341 cttgattgga tattagaact agcgttctga tcttgtcaag gttttagctt ttgggttttg
     2401 ggttttgcga gggcgatcag ggggatggcg agcatgagga caccgaggag ggcgtcggcc
     2461 aggtggatgc cgtgctcgtg ccccagctcg atgagggttg ggccttgctc gaagcgggtg
     2521 aggttcgtga gcaacaggag accgaccagg acgacctcaa ggatcgctgc cagccagagg
     2581 cggtgcttca ccggatcgat gcgctccatg tgacctagcg ggcggccgat tcggcgagga
     2641 cctgctcgaa gaaggcgagg gtcgggacga ggccctcggc gagggagacg accggttccc
     2701 aatcgagcag gcgtttggcc ttgccgatat cggggcggcg gcgggtgggg tcgtcctgcg
     2761 ggaggggttt gaagacgagc tccgacgtcg agccggtgat cttgaggatc tcctcggcga
     2821 actggagcat ggtgaactcg accgggttgc ccatgttgac gacctcgccg gccagcccct
     2881 cgccctccat gaagaggacg aagccgcgga tgagatcgtt cacatattgg aaggagcggg
     2941 tctgctggcc gctgccgaag acggtgatcg gctcaccgcg gagggcttgg acgcagaaat
     3001 tgggcacgac gcggccgtcg ctggggtggg agcgggggcc gtaggtattg aagatacgga
     3061 cgatgcggac atcgaggccg tgctggcggt ggtattccat gatgaggctt tcggcgaagc
     3121 gcttgccttc gtcgtagcag gcgcgggggc cgttggggtt gacgtggccc cagtagtcct
     3181 cggtctgggg gtggacctgg gggtcgccgt agacctcgga ggtggaggcc tggaagaagc
     3241 gggcgccgtg tgcgcgggcg agctcg
//
LOCUS       DASSGX010000460         7206 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_953507, whole genome shotgun sequence.
ACCESSION   DASSGX010000460 DASSGX010000000
VERSION     DASSGX010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 7206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7206
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953507"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            271..1227
                     /locus_tag="VFI12_08210"
     CDS             271..1227
                     /locus_tag="VFI12_08210"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET7056432.1"
                     /translation="MTEWNRWPASSRQMGMGVMLPIAEKSAFGDRPVRFADMVEMART
                     AEDAGYDAIWLADHFLFRPPVAKEGDEYGLWEAWTAAAGLAQATSKIQIGLLVTCLGW
                     RNPGIVARMTDTIDEISGGRFILGVGAGWHEPEYTAYGMPFDHRYSRFEDAITILDGL
                     LRHGRADHDGRFFSANDAISQPRGPMGAKGGSPILVGSSSPKMLRLVAKYADAWNTVW
                     HSDASATLEDLKALDAALAEAGRDPGTIVRTAGGNIAQPGYTGTRGSPIEGDNDHIAG
                     KLAEFRDLGFRHYVAGLDPCTPTSIEQFARVIEILDKTGAPA"
     gene            1224..2138
                     /locus_tag="VFI12_08215"
     CDS             1224..2138
                     /locus_tag="VFI12_08215"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET7056433.1"
                     /translation="MTRPIKIGIMLPESEYEMAGQTAGWSDFAAMARTTEELGFDSIW
                     FADHLQLKLPDGRDQGAWECWTILSAIAATTSRIELGPFVTATAYRNPALLARTAETL
                     DEISGGRLIFGIGAGWAEHEFRVHDWPYDHRASRFEEALTIITSLIRTGSVDFHGDYY
                     TVREALLRPRGPRPEGMPIMIGTFSGERMMRLAAKEADHWNIWANAFGNRAESLAPLQ
                     AKMDEYCREVDRDPATLTRSASVLVDFDNPYGRPGQPVPSLTGDARALADEYLRFAEA
                     GVDHLQLYPDPCTVDGIKRCAEALEYLD"
     gene            2409..3056
                     /locus_tag="VFI12_08220"
     CDS             2409..3056
                     /locus_tag="VFI12_08220"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HET7056434.1"
                     /translation="MPITIDVVQDTVCPWCRIGKKNLDDALKHFDGETGVRFHPYFLR
                     PEMPQAGRDFREHMRAIKGDDDIEPMLIRVSNTGLQAGLTFNWDKVKRMPNSLLSHAL
                     ILSAPVEAQGRVLDAVHQAYFVDGKDIGKKETLIEISDGLPVDITKLDDEAFLDEVAG
                     RAEAAQAQGITGVPFFIIDGQVGISGAQPPSVLLEAMEEAVEARRREGAEARSNL"
     gene            complement(3288..4142)
                     /locus_tag="VFI12_08225"
     CDS             complement(3288..4142)
                     /locus_tag="VFI12_08225"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7056435.1"
                     /translation="MPSNLFELARPILGNPPADLAYIDSGLSGASPARVFVDDPERPT
                     TALMARTYEYYVGGARETPLADFIHDAPAEAGIWADFYGFVAVDHEWDDQLRALHPDL
                     ETIGRRSFRFHRDHLDRARGWRDRVPLGLRVVPMTAELAEKADREMPEVIGLIWAGYE
                     RFGERGFGALMLDGDRPVSMCCTFGVGAGEANLGVMTVPDYRRRGLATICSHAAIEMA
                     FDRGLEATWDCDQPNVASGALALAIGFTEHPPFTELAFPKRAKPQQTPRLWTSAPAEN
                     GVVRWSRM"
     gene            complement(4416..6716)
                     /locus_tag="VFI12_08230"
     CDS             complement(4416..6716)
                     /locus_tag="VFI12_08230"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET7056436.1"
                     /translation="MSRNDTETNEALDLDRRWDELNATNPAVRQLDRGDQELRELAEL
                     DPSEPPRLEFVAGLWSGLRDEARRTGPMEQPARDLPGQPLEPVRSVVRMRRMRGMSGA
                     NKVASLLLAASLLVGMVAALLRYPFTNQGGTGGPTVVAPGPAIPDVPMPGANPARTNV
                     QPGPGLTMLPEIKQKADIVGTSMALADNVLVVVTYSTVSAFDVNTLTELWSVKPEPGS
                     YSAPAIANGVMYFGYTKDFESGRDVPDNDQLVAMSLADGRELWRVTGAGVYPASPVVF
                     DSVVYALGGTNQQYTLGAYQAGDGTQLWRTDVAAHGFCCPDIGIAVANGRVAVSLSDG
                     VTPSLKQMDEPGVINYLVGTTNPHTVGVFDAGDGSRLWLDSDSSGGTVGEPVIAGDLL
                     IVNDARETWSGPPNEGIEVTGGATTAYDLATGAVRWSNDAARSHRLPVALAAGSNAVL
                     YAGPWQDSDGGQLALLSLGNGQPHWNVAMPQVPGDKIVYAPSDVPPVVVGDSAYVVGS
                     TEMTPGGAIQSSLLSAIDLATGHVQWMAQIDGGIVSAPIVSGGRIFVLTHDAGFYVLG
                     DTSALMATPTATAGAVVDLRTPVVCTAAPSDSPMLGNLPATPTLPEVAPWHQSIPFSS
                     IPSGVASNVDAATAQQLEALFDAYRACSAVDPYQGVFGFFSTDFYVRLKTVQEYWGEP
                     DQPWAVWMAASFVYLKLDTSSLQMLPDGRISGLVNGPTTNVYVWFAQEDGQWKIDEYH
                     PIAPDRSTPVVGTPAPSTNSEGTPLG"
     gene            complement(6713..>7206)
                     /locus_tag="VFI12_08235"
     CDS             complement(6713..>7206)
                     /locus_tag="VFI12_08235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7056437.1"
                     /translation="LYNRYVEPVFRYCHRRLPQAAAEDATSITFLNAIRAIRGFDPER
                     AGFRSWLFTIAHNAVIDQLRARPHVQIDEIELAEPGPSLDDRVIADERRRHFAHALRR
                     LNPDQQQVIGLRLSGLSGTEIAEVMGKRPGAVRVIQHRAIKELRRLMGVRESGSANRT
                     TAR"
BASE COUNT         1243 a         2442 c         2378 g         1143 t
ORIGIN      
        1 cattgatcgg cgcggtggtc gtttgcgcca tcgccgtctt cctcagtacc tggcgtttac
       61 ggaagatcaa tatcgactag ccccggcgcc gtacaaggga cgtagcgcgg tccccccgtg
      121 agccgccgga accccctgcc gacgcgatcg gtgccgggca ccacccgccg tgaccgggtt
      181 cattgccatg caccgtgtgg gtggaggacc aatgcggccc acggggggac cgcgctacga
      241 ttccggcatg atccgtacag aggaggcaac atgaccgaat ggaaccggtg gccggcatcg
      301 agccggcaga tgggcatggg cgtgatgctg cccatcgccg agaagagcgc cttcggggac
      361 cgcccggtcc gattcgccga catggtcgag atggcccgaa ccgccgagga cgccggatac
      421 gacgcgatct ggctggccga ccacttcctc ttccgcccgc ccgtcgccaa agagggcgac
      481 gaatacgggc tctgggaagc ctggactgcc gctgccggcc tcgcccaggc gacttcgaag
      541 atccagatcg gcttgctggt cacctgcctc ggctggcgca accccggcat cgtcgcccgc
      601 atgaccgaca cgatcgacga gatcagcggc ggccgcttca tcctcggcgt gggcgcgggc
      661 tggcacgagc cggagtacac cgcctacggg atgcccttcg accaccgcta cagccgcttc
      721 gaggacgcga tcaccatcct cgacggtctg ctgcggcacg gccgggcgga ccacgacggc
      781 cgcttcttca gcgcgaacga tgcgatcagc cagccgcgcg ggccgatggg cgcgaagggc
      841 ggttcgccga ttctggtcgg cagcagcagc ccgaagatgc tccgcctcgt cgcgaagtac
      901 gccgacgcct ggaacacggt ctggcacagc gacgccagcg cgacgctcga ggacttgaag
      961 gcgctcgatg cagccctggc cgaggccggg cgcgacccgg gcacgatcgt ccgcaccgcc
     1021 ggcggcaaca tcgcccagcc cggctacacc ggtacgcgcg gcagcccgat cgagggtgac
     1081 aacgatcaca tcgccgggaa gctggccgag ttccgcgatc ttggcttccg ccactacgtc
     1141 gccggactcg acccgtgcac gcccacgtcg atcgagcagt tcgccagggt gatcgaaatc
     1201 ctcgacaaga ccggagcccc ggcatgaccc ggccgatcaa gatcgggatc atgctgcccg
     1261 agtccgagta cgagatggcc gggcagaccg ccggctggtc cgacttcgcg gcaatggcgc
     1321 gaacgactga ggagctcgga ttcgactcga tctggttcgc cgaccatctc cagctgaaac
     1381 tgccggacgg gcgggaccag ggcgcgtggg agtgctggac catcctctcg gcgattgccg
     1441 cgaccacgag ccggatcgag ctcggcccgt tcgtgacggc caccgcctac cgcaacccgg
     1501 ccctgctcgc ccggaccgcc gagacgctcg atgagatcag cggcgggcgg ctgatcttcg
     1561 ggatcggcgc cggttgggcc gagcacgaat tccgcgtcca cgactggccc tacgaccacc
     1621 gcgcgagccg cttcgaggag gcgctgacga tcatcaccag cctgatccgg accggtagcg
     1681 tcgactttca tggcgactat tacaccgtcc gggaggcgct gctgcgcccg cgcggtcccc
     1741 ggccagaggg gatgccgatc atgatcggca ccttcagcgg cgagcggatg atgcggctgg
     1801 cggcgaagga ggcggaccac tggaacatct gggccaacgc cttcggcaac cgggccgaat
     1861 cactcgctcc gctccaggcg aagatggatg agtactgcag ggaggtcgat cgcgatccgg
     1921 cgacgctgac ccggagcgcg tcggtactgg tcgatttcga caacccgtac ggccggccgg
     1981 gccagccggt cccctctctg accggggacg cgcgggcgct ggcggacgaa tacctccgct
     2041 ttgccgaggc gggcgtcgac cacctccagc tctacccgga cccttgcacg gtcgacggca
     2101 tcaagcgctg cgccgaggca ttggagtatc tggattaggc cgagctggtt cgcgagcacg
     2161 caccccgaaa cgtagcgtcg ggttaccatg cccggcgatc gccaataggg cgatgatggt
     2221 gttgccggag gatctggctc cgaaacgcga tcggttcgcc cggcgcccgg ggataaatcc
     2281 ccgggctaca tcaaataagc cagctgaagc aggctacgaa tcagtcggtt tcaaccgact
     2341 tccgtcaatg agacggggat ttcaatcccc ggcaacgtcc gtgacgggcg tcaaatagag
     2401 ggacgcaatt gccaattacg attgatgtcg ttcaggacac ggtctgcccg tggtgccgga
     2461 tcggcaagaa gaatctcgac gacgcgctca agcacttcga cggcgagact ggggtgcggt
     2521 tccacccgta cttcctccgg cccgagatgc cccaggccgg gcgcgatttc cgcgagcaca
     2581 tgcgcgcgat caagggcgac gacgacatcg agcccatgct gatccgcgtc tcgaacaccg
     2641 gattgcaggc cggcctgacc ttcaactggg acaaggtcaa gcggatgccg aactcgctcc
     2701 tctcgcacgc gctcatcctc tccgctccgg tcgaggcaca gggccgggtg ctcgacgccg
     2761 tccaccaggc ctatttcgtc gacgggaagg acatcggcaa gaaggagacg ctgatcgaga
     2821 tcagcgatgg tcttccggtg gacataacga agctggacga cgaggcgttc ctcgacgaag
     2881 tcgccggcag ggccgaagcc gcccaggccc agggcatcac cggcgtcccc ttcttcatca
     2941 tcgatggcca ggtcggcatc tccggcgccc aacccccaag cgtcctgctg gaagcgatgg
     3001 aagaggcggt tgaggcgcga aggcgcgaag gcgcggaggc gcgaagcaat ttgtaggggt
     3061 ttgcgggggc aaacccgtgc ctccccgacc ggtccgtctc ctgatgccgg tcccggtgta
     3121 ggggcgtgcc gggccacgcc cgcgcctccc cccagcgcgt gacggcattg ggggcattcc
     3181 cggttcaccc tatccgacgg gctcggggaa ccacaacgga tgtgctcccg cacatcccta
     3241 cagctgaccg gtccttcagc aatgtccagg tttgctgtcg caaaccccta cattcggctc
     3301 cagcgaacga cgccattctc ggccggtgcg ctggtccaaa ggcggggcgt ctgctgcggt
     3361 ttggcgcgtt tgggaaaggc gagctcggtg aagggtgggt gctcggtgaa gccgatcgcg
     3421 agggcgagcg ctcccgatgc gacgttcggc tggtcacaat cccacgtcgc ttcgaggcca
     3481 cggtcgaacg ccatctcgat tgcggcgtgg ctgcaaatgg tcgccaggcc gcggcggcgg
     3541 tagtccggaa ccgtcatcac gccgaggttc gcctcgcccg cgccgacccc gaacgtgcag
     3601 cacatcgaaa ccggcctgtc gccatcgagc atcagcgcgc cgaagccacg ctcgccgaac
     3661 cgctcgtagc cggcccagat cagcccgatc acctccggca tctcccggtc cgccttctcg
     3721 gccagctccg ccgtcatcgg tacgacgcgg agcccgagcg ggacgcgatc ccgccagccc
     3781 cgggcgcggt cgaggtgatc gcggtggaag cggaaggagc gccgcccgat cgtctcgagg
     3841 tccggatgca gggcgcggag ctgatcgtcc cattcgtgat cgacggcgac gaagccgtag
     3901 aaatcggccc agatcccggc ctcggccggc gcatcgtgga tgaagtcggc cagcggcgtc
     3961 tcgcgcgcgc cgccgacgta atactcgtag gtccgcgcca tcagggccgt cgtcggtcgc
     4021 tcgggatcgt cgacaaagac ccgggccggg ctcgccccgg acagccccga gtcgatatag
     4081 gccagatccg ccggcggatt cccaaggatc ggccgggcaa gctcgaacag gtttgacggc
     4141 aactcataga catccatacc cggattctac gggtttgccg ccacaaaccc gcgcgacgcc
     4201 taatcgcccg ttgccatacg cccggttgta ggggtccgcg ggggcggacc cgccgctggc
     4261 cgaatcgggg ccttgggagg cggcggtcac gggggatcac cacggcccct catgcgaact
     4321 ggccggctga tgagacgtgg gcgtgccccc gcacgcccct acatcacgat tcgttccagc
     4381 ccaagcgtaa acatccgggc gttgcgcgcc cgccgtcacc cgaggggagt gccctccgaa
     4441 ttggtggagg gcgccggcgt gccgactacc ggtgtggagc ggtccggtgc aatcggatga
     4501 tattcatcga tcttccactg gccgtcttcc tgggcaaacc acacgtagac gttggtcgtt
     4561 gggccgttga ccaagccact aatccgccca tccggcagca tttgcagcga actcgtatcc
     4621 agcttcaggt agacgaacga ggccgccatc cagactgccc acggctgatc tggctcgccc
     4681 caatactcct gcacggtctt gagccgcacg tagaagtcgg tcgagaagaa gccgaagacg
     4741 ccctggtagg gatcgactgc ggagcacgct cggtatgcat caaacagggc ttcgagctgc
     4801 tgtgcggtcg cggcgtcgac gttacttgcg acgccggacg gaatcgaaga gaacgggatc
     4861 gactggtgcc agggcgcaac ctccggaagc gtcggcgtgg ccggcaggtt gccgagcatc
     4921 gggctgtcgg atggcgcggc tgtgcagacg accggcgtcc gcaggtcgac cacggctccc
     4981 gcagttgctg tgggggtagc catcagcgcc gaggtgtcgc ccagtacgta gaacccggcg
     5041 tcgtgcgtca acacgaatat ccgccccccg gaaacgattg gcgcggagac gatgccaccg
     5101 tcgatctgcg ccatccattg cacgtggccg gtcgcgagat cgatggcgct gaggaggctg
     5161 ctttgtatcg cgccgcccgg cgtcatctcc gtggaaccga ccacataggc ggaatccccg
     5221 accacgacgg gcgggacatc gctcggcgcg tagacgatct tgtcgcctgg aacctgcggc
     5281 atggcgacgt tccagtgcgg ttgcccgttc ccgagcgaga gcagcgccag ctgaccaccg
     5341 tccgaatcct gccaaggtcc cgcgtagagc accgcattcg accccgcggc taacgcaacc
     5401 ggaaggcgat gggaacgcgc ggcatcgttc gaccaccgga ccgcgccggt tgcgaggtca
     5461 taggctgtgg tcgcgccgcc ggtcacttcg atcccctcgt ttggcgggcc cgaccaagtc
     5521 tctcgtgcgt cattgacgat cagcaggtca ccggcgatga ccggctcgcc gacggtcccg
     5581 ccactggagt ccgagtccag ccagagccgg gagccatcgc ccgcatcgaa gacgccgacc
     5641 gtgtgcgggt tagtggtgcc gacgaggtaa ttgatgaccc ccggctcgtc catctgtttg
     5701 aggctcggcg tgacgccatc ggacagcgaa accgccactc taccgttggc aaccgcaatg
     5761 ccgatatccg ggcaacagaa gccgtgggcg gcaacatcgg tccgccacag ttgggtgccg
     5821 tctccggcct gataggcgcc caaggtgtac tgctgatttg tgcctccaag ggcgtagacc
     5881 acgctatcga acacgaccgg gctggcggga tagacgcccg cgccggtgac gcgccacagc
     5941 tctcgaccgt cggccaggga catggcaacg agttgatcgt tgtccggaac gtcccgcccg
     6001 ctctcgaaat ctttcgtata gccgaaatac atgacgccgt tcgcgatcgc cggggcggag
     6061 tacgagccgg gctccggctt cacagaccag agctcggtga gcgtgttcac atcgaacgcg
     6121 ctgacggtcg aataggtgac gaccacaagt acattgtcgg ccaaggccat cgaggtcccg
     6181 acgatgtccg ccttctgctt gatctcgggc aacatggtca ggccggggcc gggttggacg
     6241 ttggtgcggg ccgggtttgc gcccggcatc ggcacgtccg gaattgccgg gccgggcgcg
     6301 acgaccgtcg gtccgccggt cccgccctgg ttcgtgaacg ggtagcggag cagcgccgcg
     6361 accattccga ccagtagcga cgccgccagc agcagcgagg cgaccttgtt cgctccggac
     6421 atcccgcgca tccgccgcat ccggacgacc gagcggaccg gctccagcgg ctgcccgggc
     6481 aaatcccgcg ccggctgctc cattgggccg gtccgtcgcg cctcgtcccg cagtccgctc
     6541 cagaggccgg ccacgaactc caatcggggc ggctccgagg ggtccagttc ggccagctct
     6601 cgcagctcct gatcgccgcg gtccaactgt cgcactgccg gatttgtcgc gttcagctcg
     6661 tcccagcggc gatcgaggtc cagcgcctca ttcgtttccg tatcgtttct gctcatctcg
     6721 ctgtcgtcct gttcgcggaa ccggattccc gcacgcccat cagtcgtcgc aattccttga
     6781 tcgcccggtg ctggatcacc cggaccgcgc cgggacgctt gcccatcacc tcggcgattt
     6841 ccgtgccgct taggccggaa agccgtagcc cgatcacctg ctgttggtcc gggttgagcc
     6901 gccgcagtgc gtgcgcaaag tggcgacggc gctcatcggc gatcacgcgg tcatcgagcg
     6961 acggccccgg ctcggccagc tcgatctcgt cgatctggac atgcggccgg gcgcgaagct
     7021 ggtcgatgac cgcgttgtgg gcgatggtga agagccagga gcggaaaccg gcgcgttccg
     7081 gatcaaaccc gcggatcgcc cggatcgcgt tcaggaacgt gatgctggtc gcgtcctcgg
     7141 ccgccgcttg gggcagccgc cggtggcaat accggaagac cggctcgaca taccggttgt
     7201 agagat
//
LOCUS       DASSGX010000461         3990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_408323, whole genome shotgun sequence.
ACCESSION   DASSGX010000461 DASSGX010000000
VERSION     DASSGX010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3990
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408323"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VFI12_08240"
     CDS             <1..215
                     /locus_tag="VFI12_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056438.1"
                     /translation="ARTDIPIILIAGSPNEISAEHAIHIGADDVTYAPIEMEELAVAL
                     FSRIREWRAQRPDRPWADQLAQRKSQ"
     gene            complement(209..874)
                     /gene="phoU"
                     /locus_tag="VFI12_08245"
     CDS             complement(209..874)
                     /gene="phoU"
                     /locus_tag="VFI12_08245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HET7056439.1"
                     /translation="MTRENYEDQLRRLRNDVVLMSGMVNAAIDRSVEALRRQDLHLAQ
                     EIIDEDRAINQARWRIEEHALLLIATQAPMARDLRMISAVLHIATELERMADHAAGNA
                     KFVMETADEPLLKPLVDLPRMAMLCRSMLDDSITAFIDLDESLARAIARRDDDVDELY
                     NQIYRELLTYMMADPGTINRATHLLWAAHNLERIADRVTNICERVVFAATGSLEEINL
                     STY"
     gene            complement(895..1740)
                     /gene="pstB"
                     /locus_tag="VFI12_08250"
     CDS             complement(895..1740)
                     /gene="pstB"
                     /locus_tag="VFI12_08250"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HET7056440.1"
                     /translation="MITTLDRPTRSPRIEVHPGVAPAIDSPIAPVFDIRNLDVSYGQA
                     RAVSDVSFTVPSNRVTALIGPSGSGKSTVLRSLNRMNDLVDTARVQGQVLYHGVDVND
                     SRVDPVAVRRRIGMVFQKPNPFPKSIYDNVAWGVKINGLKVNMDEYVEQCLRQAALWD
                     EVRDKLKQSGLALSGGQQQRLCIARAIAVNPDVILMDEPCSALDPISTLRIEDLMREL
                     IKAYSIVIVTHNMQQAARVSDKTIFFSLNEQREGILVESSPTQELFTNPKDKRTEDYI
                     TGRFG"
     gene            complement(1737..2636)
                     /gene="pstA"
                     /locus_tag="VFI12_08255"
     CDS             complement(1737..2636)
                     /gene="pstA"
                     /locus_tag="VFI12_08255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HET7056441.1"
                     /translation="MSAVTDPTGLLSASAQLKSRKMRGAIMRWLFLAATWSGLVALSV
                     LLWTILARGVPWLSWHLITDMPSRKPELAGLNSALFGTIWVISLTALFAFPLGVGAAV
                     FLEEYAPKNRLTRLLNLNISNLAGVPSIVYGLLGLGIFVQVLQIGRVILAGALTLALL
                     SLPVIIIATQEAIRAVPISLRQAAYGVGATRWQVVRSHVLPAALPGILTGTILSISRA
                     IGETAPVLVVGASAYIATRPSSFFDGYTVLPMQIYGWTQRSQDEFKNGLAPAGIIVLL
                     AVLLVMNAAAIILRQKYSNRRNA"
     gene            complement(2633..3589)
                     /gene="pstC"
                     /locus_tag="VFI12_08260"
     CDS             complement(2633..3589)
                     /gene="pstC"
                     /locus_tag="VFI12_08260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET7056442.1"
                     /translation="MQIESTPHAGAIQPEQLARSLMRSGSQSTSERVIAVILGACAAV
                     SVLTTIGIVVILFEQAIQFFRSVPVQDFLFGTKWTALFKNGKFGVLPLAAGTVFIAAL
                     SMVVAVPLGLMSAIYLSEYASARTRSLLKPGLELLAGIPTVVYGYFALTFLTPHLLQK
                     IWPETAPYNVLAGSIAVGIMVLPMIASLSEDAIRSVPGSLREAAYGLGATRFEVSTRV
                     VVPAALSGIVSAIILAVSRALGETMIVALATGSKAQLVRDPREPMQAMTGYIVQVLSG
                     DVARGSTVYYSLFAVGLLLFCMTLVMNVLSQWFVARFREEYQ"
     gene            complement(3651..>3990)
                     /locus_tag="VFI12_08265"
     CDS             complement(3651..>3990)
                     /locus_tag="VFI12_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056443.1"
                     /translation="SYFEDNQDRLKAVPIDGGNGPVAPSPETVQDLSYTPLSRPLFLY
                     VNAQSLTRPEVQEFMRYGVAEVTPLLPEVGFIGSPAHIYAQDQTRLEAAIAGNGQPDG
                     PAAPGATPSS"
BASE COUNT          765 a         1241 c         1286 g          698 t
ORIGIN      
        1 ccgcccgtac cgatatcccg atcatcctga tcgccgggag cccgaacgag atcagtgcag
       61 agcacgcgat tcacatcggc gcagacgacg tcacttacgc gccgatcgag atggaggagc
      121 tcgctgtcgc gctcttcagc cgcattcgcg aatggcgcgc acagcggccg gaccggccgt
      181 gggcggacca actggcgcag cggaagtctc agtaggtcga caggttaatc tcttcgagtg
      241 atccggtggc cgcaaacaca acccgctcgc agatattcgt gacccgatcg gcaattcgct
      301 ccagattgtg ggcggcccag agcagatgag tggcacgatt gatggtcccc ggatcagcca
      361 tcatgtacgt caggagctcc cggtagatct ggttgtacag ctcatcgaca tcgtcgtctc
      421 gtcgcgcgat cgcgcgggcc agggattcgt ccaggtcgat aaacgcggtg atgctgtcgt
      481 cgagcatgga gcggcaaagc atcgccatgc ggggaagatc gaccaacggt ttgagcaacg
      541 gctcatccgc cgtctccatc acgaacttcg cgttgcccgc ggcatgatcc gccattcgct
      601 ccagctcagt cgcgatatgg agcacggccg agatcatgcg caagtcacgc gccatcgggg
      661 cctgcgtcgc aatcaggagc agcgcgtgtt cctcaatcct ccaccgagcc tgattaatgg
      721 cgcggtcctc gtcgatgatt tcctgcgcca ggtgcaggtc ctgtcgccgc aatgcctcga
      781 ccgaccggtc gatcgccgcg ttgaccatcc cggacatcaa gaccacatcg ttccgcaatc
      841 gcctgagctg atcctcgtag ttctcgcgcg tcatcgggtg gggtctgcct cgttctagcc
      901 gaaccggccg gtgatgtaat cctcagtccg cttgtctttg ggattcgtga acagttcctg
      961 ggttggactc gactcgacca ggatgccttc ccgttgctca ttcagcgaga agaagatcgt
     1021 cttgtcggag acgcgagccg cctgctgcat gttgtgcgtg acgatcacga tcgagtaggc
     1081 cttgatcagc tcgcgcatga gatcctcgat tcgaagtgtc gagatcggat cgagcgcgct
     1141 gcacggctcg tccatcagga tcacgtccgg gttcaccgcg atggcccggg cgatgcagag
     1201 acgctgctgc tggccccccg agagggccag gccgctctgc ttgagcttgt ccctgacctc
     1261 gtcccagagt gccgcctgac gaagacactg ctcgacgtac tcgtccatgt tgaccttgag
     1321 gccgttgatc ttcacgcccc aggcgacatt gtcgtagatg ctcttcggga aggggttcgg
     1381 tttctgaaag accatgccga tccgccgtcg gaccgccacc ggatcaacgc gactgtcatt
     1441 gacgtcgacg ccgtggtaca ggacctggcc ctggactcgc gccgtgtcga ctaggtcgtt
     1501 catccgattc aagctgcgca acacagtgct ctttccgctc cccgacgggc caatcaacgc
     1561 agtcacgcga ttgctcggca ccgtgaacga gacgtcgctg acggcgcgag cctggccgta
     1621 ggagacgtcc agattgcgga tgtcgaagac cggcgcgatc ggcgaatcga tggccggcgc
     1681 gacgccggga tggacctcaa ttcggggcga acgtgtgggt ctatcaagtg tggtgatcat
     1741 gcgtttctcc gattgctgta tttctggcga aggatgatcg cggctgcatt catgaccagc
     1801 agcacggcga gcaggacgat gatccccgcc ggagcaaggc cgttcttgaa ctcatcttgc
     1861 gatcgctgcg tccagccgta gatttgcatt ggcaacaccg tgtacccatc gaagaagctg
     1921 gacgggcgcg tggcgatgta ggcggacgcg ccgacgacca gcaccggcgc cgtctcgccg
     1981 atcgcgcggg agatcgagag aatcgtcccg gtcaggatcc cggggagcgc ggccggcaac
     2041 acatgagacc ggacaacctg ccaccgagtt gcccccaccc catacgctgc ctgccggagc
     2101 gagatcggca ccgcgcgaat ggcctcctgg gtcgcgatga tgatcaccgg caggctgagc
     2161 agcgcgaggg tcaatgcgcc ggccaggatg acccggccga tttgcaagac ctgtacgaag
     2221 atgcccaggc cgaggagtcc gtagacgatc gacggcacgc cggccaggtt cgagatgttg
     2281 agattgagca ggcgggtcag ccggttcttc ggcgcgtact cctcgaggaa gaccgccgct
     2341 ccgaccccga gcgggaacgc gaatagcgcc gtcagggaga tcacccagat cgtgccgaag
     2401 agcgccgagt tcagcccggc caactcaggt tttcgggacg gcatgtcggt gatcaggtgc
     2461 cagctcagcc acggcacgcc tcgcgccagg atggtccaga gcaggacgga aagggcgacg
     2521 agtccggacc aggtcgcggc gaggaagagc cagcgcataa tcgcgccgcg catcttccgg
     2581 ctcttgagct gggcggaggc ggaaagcagc cctgtgggat ccgtgacggc gctcactggt
     2641 attcctcccg gaagcgggcg acgaaccact ggctgagaac gttcatcacc agcgtcatgc
     2701 aaaagagcag cagcccgacc gcaaagagcg agtaatagac cgtcgagccg cgcgcgacat
     2761 cgccggagag tacctgcacg atgtagccgg tcatggcctg cattggttcg cgcggatccc
     2821 tgacaagctg tgccttcgac ccggtcgcga gcgccacgat catcgtctcg cccagggccc
     2881 gggacactgc gaggatgatc gccgagacga ttcccgacag ggccgccgga acgacgactc
     2941 gggtcgatac ctcgaacctt gtcgccccca ggccatatgc cgcctcgcgc agcgaacccg
     3001 gtaccgatcg gatagcatcc tccgagagcg aggcgatcat cggcaggacc atgatgccga
     3061 ccgcaatcga gccggccagc acgttgtacg gcgctgtctc gggccagatc ttctgcaaca
     3121 ggtggggagt gaggaaggtg agggcgaagt agccatagac gactgtcgga atgccggcca
     3181 gtaactccag gccgggcttc aggaggctgc gcgtgcgcgc cgatgcgtac tcgctcaagt
     3241 agatcgccga catgagcccg agcgggaccg ccacaaccat cgatagcgcg gcgatgaaga
     3301 cggtgccggc cgccaggggc aggacgccga acttaccgtt cttgaagagg gcggtccatt
     3361 tggtcccgaa caggaaatcc tgaaccggga ccgagcgaaa gaactggatc gcctgttcga
     3421 agagtatgac gacgatcccg atcgtggtca ggacagagac cgccgcgcac gcgcccagaa
     3481 ttacggcaat gacgcgctcg gatgtcgact gggaacccga ccgcatcaac gagcgtgcca
     3541 actgttctgg ctggattgcg ccggcgtgcg gggttgattc gatctgcatt cgatccttca
     3601 gtgaacgggt gcggacccgt cagagttcgc acccattcga tgtccgttcg ctagctggac
     3661 ggggtcgccc cgggcgcagc cggtccatcc ggctgaccgt tgccggcaat cgcggcctcg
     3721 agccgtgtct ggtcctgcgc gtagatgtgc gccggcgatc cgatgaagcc gacctctggc
     3781 aggagcggcg tcacctcggc aaccccatag cgcatgaact cctgcacctc gggccgggtg
     3841 aggctctggg cgttgacgta gaggaagagg gggcgcgaga gcggggtgta tgacaggtcc
     3901 tggacggtct cggggctcgg cgcaacgggg ccgttgccgc catcgatcgg aaccgccttc
     3961 agccgatcct ggttatcttc gaagtatgag
//
LOCUS       DASSGX010000462         6286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1389292, whole genome shotgun sequence.
ACCESSION   DASSGX010000462 DASSGX010000000
VERSION     DASSGX010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6286
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1389292"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="VFI12_08270"
     CDS             <1..850
                     /locus_tag="VFI12_08270"
                     /inference="COORDINATES: protein motif:HMM:NF016736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HET7056444.1"
                     /translation="VQLRNISGKPVNLPAGTKLAMADGVAYTTTQDVAIPAGNTDKPG
                     QAEVGIRAEKPGAAANRDPGSFTGQVAGQKGVYFGNPGAGITGGTDVLIKAVTEEDLA
                     KARDQATAALSQQAQAFQLADGRVVIPSTVQPVGDPSVQSDHVAGEQIDTFTVTGQAT
                     YQALTINPADLPAQMRTDLRAQLAALVPSGYALTDDPIAFADPAEQTPGSGLLTVDAT
                     IDAAQALTPDMIAKVRDLTSGKSESDARTALASMGGVEVVDIRVTPALFVKSLPGAGK
                     IDVRAE"
     gene            847..1284
                     /gene="ruvX"
                     /locus_tag="VFI12_08275"
     CDS             847..1284
                     /gene="ruvX"
                     /locus_tag="VFI12_08275"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HET7056445.1"
                     /translation="MSPAERLMGLDVGSRRVGVAISDELGIVASPVGFIQPGPNDRAE
                     FAKLVRTHGVTRLIVGIPKNMAGKEGPQAKEVRTYATALATDLGLPMTYWDERFTTII
                     AERNLISGGRSRQQRRTEIDAAAAAVMLQGYLDHQATQRRRDE"
     gene            1347..1640
                     /locus_tag="VFI12_08280"
     CDS             1347..1640
                     /locus_tag="VFI12_08280"
                     /inference="COORDINATES: protein motif:HMM:NF017920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HET7056446.1"
                     /translation="MKKLVIAVVQNDDADNVVDALLDQDFRATRLASTGGFLRRGNTT
                     LLVGADEDQVDRVLGLIRANAHAAGLPADAQPGLTPPAAATVFVLDLDEHQRI"
     gene            complement(1637..2428)
                     /locus_tag="VFI12_08285"
     CDS             complement(1637..2428)
                     /locus_tag="VFI12_08285"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HET7056447.1"
                     /translation="MGRLPAGNQTIDERIRTAVSLIRGASSVVVMTGAGISTESGIPD
                     YRGPGGVWETQTPPTIGDYLDNIETRRGFWRRRLTDYPSLAAREPNAGHRALVELERR
                     GVLLAIITQNIDGLHQKAGNSPERVIELHGSAHRVLCVDCGRFWPSDEVQERLRNGED
                     DPRCSFCGGILRSGTVLFGESLPKAEIEKAVAVSNACDVMLVVGSSLVVNPAAQLPQI
                     ARRRGASLIMINRTGTPLDHLADVHILSEAGPTLSDVISRLDTGR"
     gene            2819..3166
                     /locus_tag="VFI12_08290"
     CDS             2819..3166
                     /locus_tag="VFI12_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056448.1"
                     /translation="MSLRDKFTAPPEAEPLSATEERARIYAVKGYLRARDGQYDQAQA
                     QFARAVDLDPELDLTTIPRFWDLPRAAHDSAVAALEQVGRGRDASRLLAIINRKFRPK
                     LVGARPRQIKPAQ"
     gene            3293..4846
                     /locus_tag="VFI12_08295"
     CDS             3293..4846
                     /locus_tag="VFI12_08295"
                     /inference="COORDINATES: protein motif:HMM:NF039815.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056449.1"
                     /translation="MPARRLSVRPFRFALPLLIVLGMLVPAFAIRTAGQSSPARGTAQ
                     VIAQGLTAPPADRVAWRVIKQPIPTRLDARPSNRMQASTGFLLADDTPVFVADQRTKL
                     RSRLAPGEAQFIPSGANQTWASLGDGASTAYSLELVDRSVVTISPDGEIVYRGGAFTM
                     KPGDYDLDLIRDDLPAGERATIEQARFPILVFVTSGQVEVTSSKQDDPVRLKAGQGIS
                     LGGTLTVRARGKDSTYVAAIMGDAVSGGQDVPTPVATNTPKPKKTSTAEPEATGTPKP
                     KKTKTPEPEATSKPKQTPTPGIDDGSSVRIAVRLCREGMTYFAIDPRGCQRADGDYQL
                     ALIAPNGKHLRMSDASRSEPSFVRWSGLKRGEYVLVVGKMPDGYKSYSLDGFICCSTN
                     DGYSITVDRNQVIDGTLYLFTSDWGVGGPPPPTPVPPQPQPQPPVNPAPGVDSDGDGL
                     SDELELDVFGTSPYLVDSDGDTIPDGVEAFGSNGYLTAPALPDTDFDGVMDNVEIQQG
                     TDPLDPSSR"
     gene            4868..>6286
                     /locus_tag="VFI12_08300"
     CDS             4868..>6286
                     /locus_tag="VFI12_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056450.1"
                     /translation="MSEQRFDQIARRLARESADRRSVLAWVLVAMSGLLPWRRVRAGC
                     VETGAACLEGDPCCQGFTCLEGLCDCPAGIFDEFGTCVALAPCRPPGDYCNPGEECCN
                     GYSCLEGICSCPGVLFDLEGNCNPEVTPCLAPGAACQLADTCCNGYSCFEGICDCPSG
                     LIDAAGICGMNTVPCAPEGVACGSDGDCCDGMDCQAGVCVRVAALICRGHGEICATDR
                     DCCPESGTACIDGKCACPTRHVLDRERMICESHCAFPGERCDDTTPCCDGAGSCIAGT
                     CTCAPGERSCGALCVNITGKFQRDVRNCGSRGHACAEVEICCDGKCWGPADLAQNPDH
                     CGRCCSACHHDQICCNGRCQDPPDDPNASQICCAEGNLCGDPAGTEQCCASGETCVGG
                     ECCPDILVCGDVCCGDGSFCQCGICVTDLATCESDTDCCDGACTNGFCCPATAVCSDF
                     EGNPICCGGTSICRTDVNICEAI"
BASE COUNT         1112 a         2026 c         2070 g         1078 t
ORIGIN      
        1 tgtgcaactc cggaacatca gcgggaagcc ggtcaatttg ccagccggga ccaaactcgc
       61 catggccgac ggtgttgcct atacgacgac ccaggacgtg gcaattcccg cagggaatac
      121 cgacaagccc ggccaggcgg aggtcggcat ccgggctgag aagccgggtg ccgcggccaa
      181 tcgggacccg ggaagcttca ccgggcaggt cgcgggtcag aagggcgtct atttcggcaa
      241 tcccggagcc gggatcaccg gcgggacaga cgtgctgatc aaggccgtga ctgaggagga
      301 cctggcaaag gcgcgggatc aggcgactgc cgcattgagc cagcaggcac aggcgtttca
      361 gcttgccgac ggccgcgtcg tcattccgtc gaccgtgcag ccggtcggtg atccgtcggt
      421 gcagtcggat cacgttgccg gagagcagat cgataccttc accgtcaccg ggcaggcgac
      481 ctatcaggcg ctcacgatca atccggccga cttgcccgcg cagatgcgga ctgatctccg
      541 cgcgcaactc gccgcgctcg tccccagcgg gtatgcgctg acagatgatc cgatcgcctt
      601 cgccgatccc gccgagcaaa cccctggttc gggcctgctc accgtcgacg ccacgatcga
      661 cgcggcccag gcactgacgc ccgatatgat cgcgaaggtc cgggacctga ccagtggcaa
      721 gtcggagtct gacgcgcgca cggccctggc gtcgatgggc ggggtcgagg tggtcgacat
      781 cagggtcacg cccgcgctct tcgtcaaatc gctaccaggc gcgggcaaga tcgacgtgag
      841 ggcagaatga gtccggcaga acggctaatg ggactggatg tcgggtcgcg ccgggtaggt
      901 gtggcgatca gcgacgagct cggcatcgtc gcgagtccgg tcggattcat ccaaccggga
      961 ccgaacgacc gcgccgagtt cgcgaaactg gtccgcacac atggtgtcac gcgcctgatc
     1021 gtcgggattc cgaagaacat ggccggcaag gaaggaccgc aggcgaagga ggtccgcacc
     1081 tatgccacag cgcttgccac tgacctcggg ttgccgatga cctattggga tgagcggttc
     1141 acgacgatca tcgccgagcg caacctgatc tcgggtggcc ggagccgtca gcagcgccga
     1201 accgagattg acgcggccgc cgccgccgtc atgctccagg ggtacctcga ccaccaggca
     1261 acgcagcgcc gccgggacga gtaatgcgac tctgctatat tcgggcaaat cgcagcgcct
     1321 gaggatcagg gtgggggaga gcacgcatga agaaactcgt catagccgtt gtccagaacg
     1381 acgatgccga taacgtggtc gatgccctgc tggatcagga cttccgcgcc acacggctcg
     1441 ccagcacggg tggcttcctg cgccgcggca acacgacatt gctggtcggc gctgacgagg
     1501 atcaggtcga tcgcgtgctc gggttgatcc gggccaatgc ccatgccgcc ggcctcccgg
     1561 ccgatgccca gccgggtctc acgccgccgg ctgccgcgac cgtcttcgtg ctcgaccttg
     1621 acgagcatca gcgcatctaa cgacccgtat ccaggcggct gatcacatcg cttagcgtcg
     1681 gcccggcctc gctgaggata tggacatcgg cgaggtgatc gagcggcgtc ccggtccggt
     1741 tgatcatgat caggctggct ccccggcgcc gggcaatctg cggtaactgc gcagccgggt
     1801 tgacgaccag cgacgatccg accaccagca taacgtcgca ggcgttgctg acggccaccg
     1861 ctttctcgat ctccgccttc ggcagcgact caccgaagag aaccgtgccc gaacgcagga
     1921 ttccgccgca gaatgagcag cgcgggtcgt cctcgccatt tctgagccgt tcctggacct
     1981 cgtccgacgg ccagaaccgg ccgcaatcga cgcacaagac ccggtgcgcg ctgccgtgga
     2041 gctcaatgac gcgctcgggc gagttgccgg ccttctggtg cagtccgtcg atgttctgcg
     2101 tgatgatcgc gagcagtact ccgcgccgct ccagctcgac cagcgcccgg tggccggcat
     2161 tcggctcgcg cgccgcaagg ctggggtagt cggtgagtcg acggcgccag aatccgcggc
     2221 gcgtctcgat gttgtcgaga taatcgccga tcgtcggcgg tgtctgcgtc tcccaaacgc
     2281 cacccggacc gcggtagtcg ggtatgccgg attcggtgct gatgccggcg ccggtcatga
     2341 cgacgacgga ggatgcgccc cgaataaggc tgacggcggt ccggatccgc tcatcaattg
     2401 tctgattgcc cgctggtaat cggcccattc gtatccttgc gacggcacta tcggccccgg
     2461 aaaagtcgtg tcccgccatt gtgtacgtac agtggcgatg ctaccctttg tgtcccgtag
     2521 tcaaatagcg gtttgcatca gggacgcgaa agcccgtcga attatctgga cctgatgccc
     2581 agacggtgcg cgccggtccg gcgtgcgggg agtgcgggca tgatcgtgac gtcctatacg
     2641 cgcctggtcg tcgagtcgca ccggagcgac tgccgaaatg cgctcggtgg acttgccagc
     2701 ctggtcggcc gccgatcatc ctcagccgcg cactccgaag cctaccgcga acgggaaggt
     2761 acaatgatcc acctgggtca gccgacaccg atcacggcgg aggtaaccga gctcgtccat
     2821 gtcgcttcga gacaaattca cggcgccgcc tgaggcggaa ccgctttccg ccaccgagga
     2881 gcgcgcgcgc atctatgccg tcaagggata cctgcgggcg cgtgatggtc agtacgacca
     2941 ggcgcaggcg caatttgcca gggccgtgga tcttgatccc gagctggatc taaccacgat
     3001 tccgcggttt tgggatctcc cgcgcgccgc acacgactcg gctgtagcag cgctcgaaca
     3061 ggttgggcga ggccgcgacg cgtcgcgact gctggccatc atcaaccgca aatttcgacc
     3121 taaacttgtt ggcgcgcgtc ctcgccaaat caagcctgcc caataacttc ctggcagtcc
     3181 ctccgcataa aacaagccgc atgggctacg ctttcgtgtc gcaccggtcc gctgcatcct
     3241 ggtttgccgc cggtccgaca cggaacacgc gcatcttttc atagaggtga tcatgcccgc
     3301 acgccgcttg tcggtgaggc cattccgctt cgctcttccc ttgctgatcg tgctgggaat
     3361 gcttgtcccg gccttcgcca tccggaccgc cgggcaatcg tccccggccc ggggcaccgc
     3421 gcaggtcatt gcgcagggac tgaccgctcc gccggccgat cgcgtggcgt ggcgagtcat
     3481 caagcagccg attccgacca ggctcgatgc gcgtccctca aatcggatgc aggccagtac
     3541 cggctttctg ctcgccgacg atacacccgt cttcgtggcc gatcagcgca cgaaactccg
     3601 ctcccgcctg gcgcccgggg aggcgcagtt catcccctcc ggcgcgaatc agacatgggc
     3661 cagcctggga gacggcgcga gcacggccta ctcgctggag ttggtcgatc gctcggtcgt
     3721 gaccatcagc ccggacggcg agatcgtcta ccggggtggc gctttcacga tgaagcccgg
     3781 cgactacgat ctcgacctga tccgcgacga cctgccggct ggagagcggg cgacgatcga
     3841 acaggcacgc tttccgatcc tcgtcttcgt caccagcgga caagtggagg taacgtcgtc
     3901 gaagcaagat gatccagtcc ggctgaaggc cggccaggga atctcgcttg gcggcacgct
     3961 gacagttcgc gcgcgtggca aggactcgac atacgtcgcg gcgatcatgg gcgacgccgt
     4021 ttccggcgga caggacgtcc cgactcccgt ggcgacgaat accccgaagc cgaagaagac
     4081 gagcacggcg gaaccggagg cgacagggac gccgaaaccg aagaagacga agacgcccga
     4141 acctgaggcg acgagcaagc caaagcagac tccgacgccc gggatcgatg acggctcgtc
     4201 ggttcggatc gccgttcgcc tctgccgcga agggatgacc tattttgcga tcgatccgcg
     4261 cggatgccag cgcgccgatg gcgattatca gcttgccttg attgctccaa acggcaagca
     4321 tctccggatg agcgacgcga gccggtcgga gccgagcttc gtgcgctgga gcgggctgaa
     4381 gcgtggcgaa tatgtgctgg tcgtcggcaa gatgccggat ggctacaaga gttattcgct
     4441 cgatggcttc atctgctgca gcaccaatga cggctactcg atcaccgtcg atagaaacca
     4501 ggtgattgac ggcacactct atctcttcac atcagactgg ggcgtcggcg ggccgccgcc
     4561 gccgacgccg gttccgccac aaccgcagcc tcagccaccc gtcaacccgg ccccgggtgt
     4621 cgattcagac ggcgacggcc tctctgatga gctggagctg gacgtcttcg gcacaagtcc
     4681 ctacctggtc gattccgacg gagacacgat tccggatggc gtcgaagcat tcggcagcaa
     4741 tggctacctg actgcgcctg ccttgccgga caccgatttc gacggtgtca tggacaacgt
     4801 cgagatccag cagggcaccg atccgctcga tccgagcagc cgatagcgca gccggcggat
     4861 ctgggcgttg agcgagcagc gattcgatca gattgcccgc cgcctggcgc gggagtcggc
     4921 ggatcgccgg tcggtccttg cctgggtgct ggtggctatg tcggggctgt tgccgtggcg
     4981 ccgtgtccga gccggctgcg ttgagacggg cgcggcctgc ctggagggtg acccgtgctg
     5041 ccaggggttc acctgccttg agggcctctg cgactgcccg gccggcatct tcgatgaatt
     5101 cggcacctgc gtggcgctgg ctccgtgccg cccgcctggc gactactgca atccgggaga
     5161 ggaatgctgt aacggctact cctgtttgga agggatctgc agttgcccag gcgtgttgtt
     5221 cgacctggag ggcaactgta acccggaagt gacgccctgc cttgctccgg gagcagcatg
     5281 ccaactcgct gacacctgct gtaacggcta ttcctgcttc gaaggcatat gtgactgccc
     5341 aagcgggctg atcgacgctg ccggcatctg cggcatgaac accgtgcctt gtgcgccaga
     5401 gggcgttgcc tgcggctcgg atggcgactg ctgcgacggc atggattgtc aggccggcgt
     5461 ttgtgtccgg gttgcggcgc taatctgccg cggtcacggg gaaatctgcg cgaccgatcg
     5521 ggattgctgc ccggagtcgg gaacggcgtg catcgacggc aaatgcgcgt gcccgacccg
     5581 gcacgtcctc gaccgggagc ggatgatctg cgaaagccac tgcgcgttcc ccggcgagcg
     5641 gtgcgatgac acgacaccgt gctgcgacgg cgcggggagc tgcatcgccg ggacatgcac
     5701 ctgcgcgccg ggcgaacgga gctgcggcgc gctctgcgtc aacatcaccg gcaagttcca
     5761 gcgggacgtg cgcaattgcg gcagccgggg ccatgcctgc gccgaggttg agatttgctg
     5821 cgacgggaaa tgctggggac cggccgacct ggcgcagaac ccggatcact gcggccgatg
     5881 ctgctctgcg tgccatcacg atcagatctg ctgcaacggc cgctgccagg acccaccgga
     5941 tgacccgaat gcgagccaga tttgctgcgc ggagggcaac ctctgcggcg atccggcagg
     6001 gaccgagcag tgttgcgcat cgggcgagac ctgcgtcggc ggcgagtgct gcccggacat
     6061 actggtctgc ggtgatgtct gctgtggcga cgggagcttc tgccagtgcg gcatctgcgt
     6121 gaccgatctc gcgacatgtg aatccgacac cgattgctgc gacggggcgt gcaccaacgg
     6181 tttctgctgc cccgcgacgg cggtctgttc cgatttcgaa ggcaacccga tctgctgcgg
     6241 cggaacgagc atctgccgca cggatgtcaa catctgcgaa gcgatc
//
LOCUS       DASSGX010000463         2231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_625821, whole genome shotgun sequence.
ACCESSION   DASSGX010000463 DASSGX010000000
VERSION     DASSGX010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625821"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="VFI12_08305"
     CDS             <1..750
                     /locus_tag="VFI12_08305"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HET7056451.1"
                     /translation="PPAGSPRLLVVSTGTVESNREQGIGSTVVLDENFATGRAFGVSG
                     TPSAVLLDGDGLVAGVPVVGGPAVIALLNGEEPAPEAPPVPRVELKVGDTAPAISLND
                     LDGNSVTLSDFAGKSTMVMFWSPSCIFCQRMLDDLHAFEANPPADAPSLLVVSSGDIE
                     PNRAAGIQSPVLIDAGFEVGKSYGASGTPSGVLVGPDGNVAAALIVGASKILSAMGYV
                     SESCQACLDECKQQGGGAACRTVCEMGGQCA"
     gene            1071..1361
                     /locus_tag="VFI12_08310"
     CDS             1071..1361
                     /locus_tag="VFI12_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056452.1"
                     /translation="MHWSHVNTTRVFRVEHRGIGTETVVLAKIRNVFAHHDTLTQYVS
                     RLLNECGTVPPAGELVLVDDDTEAVLARRDLRRAAQETRRSRQQQPRTASDE"
     gene            1674..>2231
                     /locus_tag="VFI12_08315"
     CDS             1674..>2231
                     /locus_tag="VFI12_08315"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HET7056453.1"
                     /translation="MSSAEADLVAEAVNQPPVAAAGEAQSVTDDDGDGFADVTLDGSG
                     SADPDGTIVKYLWSLDGTWLSDQAVATVKLPVGTLKVQLTVTDNSGDSANDTVRIEVI
                     AGATKPAPEPTTPPLPPAPFQVEAKQKLTEVAITWQVLPDSVPPYHVYRCVDDGTLDN
                     GLEHTAEEIDAFNWVLIREEYDKLSY"
BASE COUNT          418 a          702 c          709 g          402 t
ORIGIN      
        1 ccacccgcgg gatcccctcg cctgctggtc gtctcaaccg gcacggtcga atcgaaccgg
       61 gagcagggaa tcggatccac cgtcgtactc gatgagaact ttgcgaccgg gcgcgcgttc
      121 ggggtaagtg gcacgccatc cgccgtactg ctcgatggcg acgggttggt tgccggtgtg
      181 ccggtggttg gtggtccggc cgtgatcgcg ctgctgaacg gcgaggaacc tgcccccgag
      241 gcgccacctg tgccaagggt cgagctcaag gtcggcgata ctgctccggc gatttcccta
      301 aacgacctgg acggcaactc tgtgacgttg agcgacttcg cgggcaagtc gaccatggtg
      361 atgttctgga gtccgagctg catcttctgc cagcgcatgc tggacgatct ccatgcattc
      421 gaagctaacc cgccggccga cgctccgtcg ttgctggtgg tctcgagcgg tgacatcgaa
      481 ccgaaccggg ccgctggaat ccagtctccc gtcctgatcg atgccgggtt cgaggttggc
      541 aagtcgtacg gcgccagtgg cacgccctcc ggcgtcctgg tcggcccgga cggcaacgta
      601 gcagcagcgc tgattgttgg ggcttcaaag atcctgtcag caatgggata cgtatctgag
      661 tcgtgtcagg catgcctgga tgaatgcaag cagcaaggcg gcggcgccgc gtgccgtacc
      721 gtctgcgaga tgggcgggca gtgcgcgtag ggcccacttc cctccgctgc acgttcaggt
      781 tcaccgtgct caccaggcgg cgctcccttg cgccgatcca gatcgatacg cactggggtg
      841 cgcaaatccc ggtccgccgc agtaccttgg tactccgccg ggagggactg gtgacccatg
      901 ccgttcgctc gcagtgaccg gactttcggg ctatctataa ggagcgtgcc gatcatcggg
      961 tagccgatgg ccggggcgaa tgtaaacagg gcggattacg ggagtacacc cggagttcgc
     1021 gtagtggggc atctatggac gtgacgagct ctggtaacca ggacgaggga atgcattggt
     1081 cccacgtcaa cacgacccgt gtttttcgag tcgagcaccg tggcatcggg actgaaaccg
     1141 tcgtcctggc caagatccgc aacgtcttcg cgcatcacga cacccttacc caatatgtct
     1201 cgcgccttct caatgagtgt ggaaccgtcc cgccggccgg cgagctggtg ctcgtcgacg
     1261 atgatactga agccgtgctg gcgcggcgcg atctccgccg ggcggcccag gagaccaggc
     1321 gaagtcggca acaacagcca cgaaccgcct ccgacgagta ggacctccac cggactgccg
     1381 ggcggcccgg cttccgcaaa gaggtgaacg gacaattgac gctccggggc cgcactccta
     1441 tagtcgacag agagacgacg cgattacctc gattcgatcg gctgtgtctg cagccagcgt
     1501 ccggagccaa cacgatcggc cacatatccg ccatggagca tcacgagccc cgcaagacag
     1561 gaatccccct gagccgacga tccgttgttc ggaccggggt gcggcttgtg tacgctgtgc
     1621 cggttgtcac cgcgagtttc cgtattgctg agatgtcggc caaagccgat tccgtgagct
     1681 ctgccgaggc ggatctcgtc gcggaagcgg tgaatcaacc gcctgtcgct gccgccggcg
     1741 aggcccagag cgtaactgac gacgatgggg acgggttcgc cgatgtgacc ctcgacggca
     1801 gcggctcggc cgatcccgac ggcacgatcg tgaagtacct gtggtcactg gatggcacct
     1861 ggttgtccga tcaggcggtc gcgaccgtca agctcccggt cggtactctc aaggtacagc
     1921 tcaccgttac cgacaactcg ggagactcgg ccaatgacac ggtccggatc gaggtcattg
     1981 ccggcgcgac caaaccagca ccagaaccaa ccacgcctcc ccttccgcca gccccattcc
     2041 aggtcgaggc gaagcagaaa ctcacagagg tcgcaataac ctggcaggtt ctgccagatt
     2101 ccgtgccgcc ctaccacgtg tatcgctgcg tggatgacgg aacgctcgat aatgggctcg
     2161 agcacaccgc ggaagagatt gacgcgttca actgggtcct gatccgcgag gaatatgaca
     2221 agctctcgta c
//
LOCUS       DASSGX010000464         3079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_954424, whole genome shotgun sequence.
ACCESSION   DASSGX010000464 DASSGX010000000
VERSION     DASSGX010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954424"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="VFI12_08320"
     CDS             complement(<1..536)
                     /locus_tag="VFI12_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HET7056454.1"
                     /translation="MTTKLVILGLPSSGKTTVFNALTRSDAPTGGFSSAADEPNLATV
                     KVPDPRLDTLTGMFNPQRKVQADVQYLDVAGVAKGMAEKGMSGQLLGHLAQADALVHV
                     VRAFEDPNLPHPEGSVDAARDIETINLELAFSDLAIIEKRLQRIAAQLPKMRGAEREA
                     YEREQAAMETLKQALENET"
     gene            complement(616..1107)
                     /locus_tag="VFI12_08325"
     CDS             complement(616..1107)
                     /locus_tag="VFI12_08325"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HET7056455.1"
                     /translation="MIDPNLTGRERVEAALENLKLNAEIVSPGTPMPTVPLAAEAVGC
                     SVEQIIKTVVFASPVGSPVIAIANGTNRIDRKRLAAAVHLDSLQLADPQFVLETTGYE
                     AGGVAPIGIRATGAPVVIDPAVLAQQSVYGGAGTEKDLLHIATTDLSRVTGACICPIT
                     RQD"
     gene            complement(1110..2021)
                     /locus_tag="VFI12_08330"
     CDS             complement(1110..2021)
                     /locus_tag="VFI12_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7056456.1"
                     /translation="MTTTTAIPIRGAAEPLTSVKRRTPAGDAIRTLRQRPVALLGLGI
                     LLAWIALGLLAPVLPLEAQNGQIKIENKLESPSWEHPFGTDDAGRDIFARVIYAARIS
                     VPAGFLVVICAATMGCLLGAASGYVGGLFDTVIMRACDGVLAFPSIILAMAIAVIRGE
                     PSLQNAMFAVVLVLWPEYARLMRGQVLSIKYNDYVTAAEAIGGTRSRVLFRHIIPSTD
                     APIVVKATLDVGAAIVLTAGLSFIGLGAVPPAAEWGSMVSQASKTGVQYWWYHTFPGL
                     AIVSVVMALNFFGDGLRDALDPKRRAR"
     gene            complement(2032..3054)
                     /locus_tag="VFI12_08335"
     CDS             complement(2032..3054)
                     /locus_tag="VFI12_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7056457.1"
                     /translation="MGLWQFIARRLVQSLVVLFVVSIATFTLMHAVPGDPLVAVIGER
                     QADRPEIRAALEKRYALDKPLPVQYVEYVRNIVVDRDLGTTITTRRPVAGELGKFIPA
                     TIELSIGAMIFALGVGIPLGIISAVRQNKWQDILARFVSLIGTAMPVFWLGLLLSYLI
                     SFKLRWLPRSGFKDTGMKIPDRVTGFLTVDSVIAGDWTFLESYLRHMILPAIVLGSFA
                     MGIVARMLRSSLLTALQDDYVRTARAKGLSEFSVVRRHALRNALIPTVTVIGLTFASL
                     LAGAVLTETIFSWPGIGQFAVQMALKLDYPGLLGVTLFVAVAYILVNLAVDILYGFLD
                     PRIRAG"
BASE COUNT          567 a          981 c          998 g          533 t
ORIGIN      
        1 gtctcgttct cgagcgcctg cttgagtgtt tccatggcgg cctgctcgcg ctcgtatgct
       61 tcgcgttcgg cgccccgcat ctttggaagc tgcgcggcga tgcgctggag ccgcttctcg
      121 atgatcgcca ggtcgctgaa cgccagctcg aggttgatcg tctcgatatc gcgcgccgca
      181 tcgaccgatc cctccggatg cggcaggttc ggatcctcaa acgctctgac gacgtggacg
      241 agggcatcgg cctgcgcgag gtgaccgagc agctggccgc tcatgccctt ctcggccatc
      301 cccttcgcga caccggcgac gtcgaggtac tggacatcgg cctgcacctt tcgctgcggg
      361 ttgaacatcc cggtcagcgt gtccagccgc ggatccggca ccttgaccgt cgccagattc
      421 ggctcatccg ccgccgagga gaaaccaccg gtcggtgcgt ccgagcgggt cagggcgttg
      481 aaaaccgtgg ttttgccgga cgaaggcaag ccgagaatca ccagtttcgt cgtcacaact
      541 cgtactttcc gtggagacgc gggcgcccgc tctcgcgcgg gccggccaat tatcggccag
      601 atcagccacg agcggctaat cctgcctcgt gatcgggcag atgcacgcgc cggtgacgcg
      661 actcaggtcg gtcgtggcga tgtggagcag gtctttctcg gttcctgcgc caccgtagac
      721 cgactgctgc gcaagcacgg ctggatcgat gacgaccggc gcgcctgtcg cccggatccc
      781 gatcggagca acgccgcctg cttcatatcc ggtcgtttcc agcacgaact gcggatcggc
      841 cagctggagc gagtcgaggt ggaccgccgc cgccagccgt tttcggtcga ttcggtttgt
      901 gccattcgcg atggcgatga ccgggctacc gacgggagag gcgaagacga cagtcttgat
      961 gatctgctcg actgaacagc cgactgcttc ggccgcgaga ggaacggtcg gcatcggcgt
     1021 gcctggcgac acgatctcag cattcagctt cagattctcg agcgcagcct ccacccgttc
     1081 tcgcccggtc agatttggat cgatcatcct taacgggccc gtcgcttggg atcgagggcg
     1141 tcccggagac cgtcgccgaa gaagttgagc gccatgacaa cgctgacgat cgcgaggccg
     1201 gggaaggtgt ggtaccacca gtattggacg ccggtcttcg atgcctgact gaccatcgag
     1261 ccccattcgg cggccggagg caccgcgccg agaccgataa agctgaggcc ggctgtcagc
     1321 acgatggccg cgccgacatc gagggtggcc ttgaccacga tcggcgcgtc ggtgctcggg
     1381 atgatgtgac gaaagagcac ccgcgatcgg gtgccgccga tcgcctcggc ggcggtcaca
     1441 taatcgttgt acttgatgct gaggacctgg ccccgcatca gacgggcgta ctccggccaa
     1501 aggacgagca cgaccgcgaa catcgcattc tgcaggctgg gctcgccgcg gatgacagcg
     1561 atggccatcg ccaggatgat cgaggggaag gcgaggactc cgtcgcacgc ccgcatgatc
     1621 acggtatcga acagaccgcc gacataccct gatgcggcgc cgaggagaca ccccattgtg
     1681 gccgcgcaga tcaccacgag gaagccggcc ggaactgaga tccgcgcggc gtagatcacc
     1741 ctcgcgaaga tgtcccggcc ggcatcgtcc gtgccgaatg gatgctccca gcttggcgat
     1801 tcgagcttgt tctcgatctt gatctggccg ttctgcgctt caagcggcag gaccggcgcg
     1861 agcagcccga gcgcgatcca cgccaacagg atgcccaggc ccagaagcgc aaccggtcgc
     1921 tgccgcagcg tccgaatcgc gtcgccggcc ggagttcggc gcttgaccga cgtcagcggc
     1981 tcggcggctc cccggattgg aatggcggtg gtggttgtca tggccatctg ctcacccggc
     2041 ccgaattctc gggtccagga acccgtagag gatatcgacg gccaggttaa cgaggatgta
     2101 tgcgacggcc acgaacaggg tcacgccgag caggcccggg taatcgagct tgagcgccat
     2161 ttgcacggcg aactggccga ttcccggcca tgagaagatc gtctctgtca ggacggcccc
     2221 ggcaagcaaa ctcgcgaagg tcaggccgat cacggtgacg gtcgggatga gggcgttgcg
     2281 cagggcatgt cgccgcacga cgctgaactc gctcagcccc ttggcccgcg cggtgcggac
     2341 gtagtcatcc tgcaaagccg tgagcagcga cgaccgcagc atgcgggcca cgatccccat
     2401 ggcgaatgag cccaacacga tcgccggcag aatcatgtgc cggaggtagc tttcgaggaa
     2461 tgtccagtcg ccggcaatga cggaatcgac ggtcaggaaa ccggtgacac gatcgggaat
     2521 tttcatcccg gtatccttga agcccgatct tggcagccat cggagcttga atgagatcag
     2581 ataggacagc aagagtccga gccagaacac cggcatcgcc gtgccgatca gcgagacgaa
     2641 ccgggcgagg atgtcttgcc acttgttctg ccgtacggcc gagatgatcc cgagcggaat
     2701 tcccacgccg agcgcgaaga tcatcgcccc gatgctcagc tcgatcgtgg ctggaatgaa
     2761 cttgccgagc tcgcccgcga ccggccgcct cgtcgtgatg gtggtgccga ggtcgcgatc
     2821 gaccacgatg ttgcgaacat actcgacgta ctgcaccggt agcggcttat ccagggcata
     2881 gcgtttctcg agcgccgccc ggatttccgg tcggtcggct tgtcgctcgc cgatcaccgc
     2941 caccaagggg tcgcccggca cggcgtgcat cagggtgaac gtcgcaatgc tcacaacgaa
     3001 gagcaccacc aatgattgga cgagtcgccg ggcgatgaac tgccagagtc ccattgcggg
     3061 cgctccttac gggtgatca
//
LOCUS       DASSGX010000465         2028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1171157, whole genome shotgun sequence.
ACCESSION   DASSGX010000465 DASSGX010000000
VERSION     DASSGX010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171157"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VFI12_08340"
     CDS             complement(<1..655)
                     /locus_tag="VFI12_08340"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HET7056458.1"
                     /translation="MVSSRPLAPTHTSPLRSLRKTTSRFDNYDLVTDPAFDPNTRRTT
                     TTDLAQHESRTHRAGSLKTSDLLPRHTGYLHGVSLSAVLQMLHLERKSCVVEVSAHGW
                     LATLTLVNGELVDAAAGDVTGEDAACLILGWHDPQTAILNAAETFRHTVQRPITQLIM
                     DAARMVDETGMLNPAKLPDFDRNPAIEASGDWQWLTDSLALAGATNIQIVTPDSMPNE
                     "
     gene            936..1379
                     /locus_tag="VFI12_08345"
     CDS             936..1379
                     /locus_tag="VFI12_08345"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HET7056459.1"
                     /translation="MELSGEHRFDVPREKVWQFLLDPDVLKASLPGCEKLEEVGPDEY
                     VATMKIGISFIRGTFTGKVKISDKNEPESYKMSVEGSGPQGQVSGVGTLNLVEDNGGT
                     IVKYVGDANVRGSLARVGARMIQPAAKTIVGQFFDKMEKEATART"
     gene            complement(1423..1983)
                     /locus_tag="VFI12_08350"
     CDS             complement(1423..1983)
                     /locus_tag="VFI12_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056460.1"
                     /translation="MDIHLRHLAAGEITDSLPETPHDLPFDRGKIDELVDRVRKGEPV
                     DLLTETLACVDWRGSFAEPDGRPITIEDIARLIAYYKQKFADVGIIYLAELLATEFIT
                     EQRARGDIIFSDRLLQLFREEPELWQEIRLFFRRKEFVTALLTQAHASRWASLNPDVR
                     RQTSDFGRQTTEDREQTSENETAGHS"
BASE COUNT          392 a          590 c          684 g          362 t
ORIGIN      
        1 gctcgttcgg catggaatcg ggcgtcacga tctggatgtt ggtcgcgccg gcaagcgcca
       61 atgaatcggt cagccattgc cagtcgcccg aagcctcgat cgccggatta cggtcgaaat
      121 cggggagctt cgccgggttg agcataccgg tctcgtcgac catccgggcg gcatccatga
      181 tcagttgcgt aatcggccgc tgaacggtgt gccggaaggt ttcggctgcg ttgaggatgg
      241 ccgtttgcgg atcgtgccag cccaggatca ggcaggctgc gtcctcaccg gtcacgtcgc
      301 cggccgctgc atcgaccaac tcgccattga cgagcgtcag tgtggcgagc cagccgtgcg
      361 ccgaaacctc gacaacgcag ctcttccgct cgaggtgaag catttgcaga acggcggaca
      421 gcgagacacc gtgcaggtag ccggtatggc gcgggagcag atccgacgtc ttgagcgagc
      481 cggcccggtg tgtcctggac tcatgctgag caaggtcggt ggtggttgtc cgccgggtat
      541 tcggatcgaa ggccgggtcg gtaacgagat cgtaattgtc gaaccgggaa gtggttttgc
      601 gaagggagcg caggggcgag gtgtgcgttg gggcaagggg tcgcgaggag accatgcggg
      661 caagcccttc gaacgcgccc ggcggcgccg ctgttgatac gagatgacct cgagcacgcg
      721 gtgcgcggcg agtcgtgagg tctcctgagg atcgtactga accgggcggc cggtggacac
      781 cgatcagaag tcccatttcg gtcacaccga atcagatcat gccgaaatcg ccgcacggcc
      841 gggccgattt cgctctggta gactgccagc ctgagagggc gcgctcccgc acgatccata
      901 aagcgcgcat cgccatcgca ccgggggatc agtcaatgga attgtcaggt gagcatcgat
      961 tcgacgtgcc gcgcgaaaag gtctggcagt tcctgctcga tcctgacgtg ctgaaggcga
     1021 gcctgcccgg ctgcgagaaa ctcgaggagg tcggcccgga cgagtacgta gcgaccatga
     1081 agatcggcat ctcgttcatc cggggcacct tcaccggcaa ggtcaagatt agcgacaaga
     1141 acgagccgga gagctacaag atgtcggtcg agggctccgg accgcagggc caggtctccg
     1201 gcgtcggcac actcaacctc gtcgaagaca acggcggcac gatcgtcaaa tacgtgggcg
     1261 atgccaacgt gcgcggctcg ctggcccggg ttggcgcccg catgatccag ccggccgcca
     1321 agaccatcgt cggccaattc ttcgacaaga tggagaagga agccaccgcg aggacgtagt
     1381 cgtcagtcgt cggacttcag acttcagttt attgacagcc ggtcaggagt gaccggctgt
     1441 ctcattttca gatgtctgtt ccctatcctc tgtggtctga cgtccgaagt ccgacgtctg
     1501 tcgtctcaca tccgggttaa gagaggccca gcggctggca tgggcctggg tcaggagcgc
     1561 ggtcacgaat tccttgcggc ggaagaagag gcggatctcc tgccagagct cgggctcctc
     1621 gcggaagagc tggagcagcc ggtcggagaa gatgatgtcg ccccgggcgc gctgctcggt
     1681 gatgaactca gtcgccagca gctccgccag gtagatgatg ccgacgtcgg cgaatttttg
     1741 cttgtagtac gcgatcagcc gggcgatatc ctcgatcgtg atcggccgac cgtccggctc
     1801 ggcaaatgag ccacgccagt ccacacaggc cagcgtctcg gtcaacaggt cgaccggctc
     1861 tcccttccgc acgcgatcga ccagctcatc gatcttgccg cggtcaaacg gcaagtcgtg
     1921 cggcgtctcc ggcagtgaat cggtgatttc gccagcagca aggtggcgca ggtgaatgtc
     1981 catggcgaga gtctagtcga gaaggcgagc agtcgaggag gcgcgaaa
//
LOCUS       DASSGX010000466         2655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_409057, whole genome shotgun sequence.
ACCESSION   DASSGX010000466 DASSGX010000000
VERSION     DASSGX010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409057"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VFI12_08355"
     CDS             complement(<1..529)
                     /locus_tag="VFI12_08355"
                     /inference="COORDINATES: protein motif:HMM:NF012912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HET7056461.1"
                     /translation="MPATIIMGGQWGDEGKGKLTDALAGRAQMVVRANGGANAGHTVQ
                     TDRGTFKMHLVPSGILNPDCACIVGAGVVLDPISFLKELDQLHEQGISTDRLKISARA
                     HVVLPYHAAFDRVQEDSLEEGKIGTTLRGIGPAYSDKAARLGLRVSDLVEPTVAATRL
                     RQLIDLKNRQLDLLYG"
     gene            complement(625..1665)
                     /locus_tag="VFI12_08360"
     CDS             complement(625..1665)
                     /locus_tag="VFI12_08360"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HET7056462.1"
                     /translation="MASSQLKDHYETLGVSRTATDKELKAAFRKLARKYHPDANRDNP
                     DAEEQFKRVNEAYEVLSDQKDRKLYDRYGDEWRAYRDAGFTGEEPRNQSRPGGQSPFY
                     RTYSYGGGGTPDEADEGAFGSIFENLFSRGGGRASTGFTQRPTKGSDLEQPIDVTFDE
                     AFRGTERRFDIQSPEACPTCGGDGFVRGAVCPRCDGTGTITRNRTIEVSIPAGVISGQ
                     RIRVKGQGSPSRTGGEPGDVYLIVNVIPDSRFERDGANLKTTVDVPLLHAVLGGEVHV
                     STPTGRVALTIPAETQNGRVFRLRGQGMPKLKTKGDRGDLLATAHIQLPQHLTDEELA
                     LFRQLRDLRAGS"
     gene            complement(1711..2307)
                     /locus_tag="VFI12_08365"
     CDS             complement(1711..2307)
                     /locus_tag="VFI12_08365"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HET7056463.1"
                     /translation="MNSGILAGIGLAAACGWNTFTPLLILALADRITGGDLLSRPFDF
                     ISSVGSILVFLALMTVELVADKVPRLDHILDVLGTVLRPATGALCFMAITDHDSSMDP
                     ILAMVFGLVIGGLVHWDKVQRRIHLAREGIGLGTPFVSVVEDFASIITAVCTVAFGIL
                     GPIAAFLNWLLVRATYRWGANFGKATLSRARSGHTLRR"
     gene            complement(2392..>2655)
                     /locus_tag="VFI12_08370"
     CDS             complement(2392..>2655)
                     /locus_tag="VFI12_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HET7056464.1"
                     /translation="RVKGLSMVDALINDGDIVLLRQQATCENGETVAVWLKDERETTL
                     KKFYHEGSRIRLQPANATMEPIYTYPGNVEIQGKLVTVIRPMA"
BASE COUNT          460 a          822 c          876 g          497 t
ORIGIN      
        1 cgccatagag cagatcgagc tgacggttct tgaggtcgat gagctgccgg agccgggtag
       61 cggcaactgt cggctcgacg aggtcagaga cgcgaagtcc gagccgtgct gccttgtccg
      121 agtaggccgg gccgatcccg cgcagggtgg tgccgatctt gccttcttcc aggctgtctt
      181 cctgcacgcg atcgaatgcg gcgtgatagg ggagaacgac gtgggctcgg gcggaaatct
      241 tgagtcgatc ggtcgagatg ccctgctcat gcagttgatc gagctccttc aggaagctga
      301 tcggatcgag cacgacgccg gccccgacga tgcaggcaca gtcggggttg aggatgccgg
      361 aggggacgag gtgcatcttg aacgtgccgc ggtcggtctg cacggtgtgg ccggcgttgg
      421 caccgccgtt tgcgcggaca accatctgcg ccctgccggc cagagcatcg gttagcttgc
      481 ccttgccctc atcgccccat tgtccgccca tgatgatcgt ggcgggcatc gtccgtcctc
      541 cctggtttgg cctgttggtg aatggcgcgg tcgcgtggta cgcgcaccga cggcgcaccc
      601 ccggaactat acccgaggcg cctgtcagga cccggcccgg aggtcgcgaa gttggcggaa
      661 gagtgccagt tcctcgtccg tcaggtgctg cggcaactgg atatgggcgg tcgcgagcag
      721 gtctccccgg tcgcccttcg tcttgagctt gggcatgccc tgcccgcgca atcggaagac
      781 cctgccgttt tgcgtctcgg ccggaatggt gagtgcgaca cggccggttg gcgtggaaac
      841 gtgcacctcg ccgccgagga ccgcatgcag caacggcaca tcgacagtcg ttttcaggtt
      901 cgcgccgtct cgctcaaatc gggagtcggg gatcacgttg acgatcaggt agacatcgcc
      961 cggctcgcca ccagtccggg aggggctgcc ctggcccttg acgcggatcc gctggcctga
     1021 gatcacgccg gcgggaatcg agacttcgat cgtgcggttt cgcgtgatcg tgcccgttcc
     1081 atcgcatcgc ggacagacgg cgccgcggac gaagccgtcg ccgccgcacg tcgggcaggc
     1141 ctcgggcgac tggatgtcga atcgccgctc agtaccccgg aaggcttcgt cgaaggtcac
     1201 gtcgattggc tgctcgaggt cggacccctt ggtcgggcgt tgggtgaagc cggtcgaggc
     1261 gcgaccgccg ccccgcgaga agagattctc gaaaatcgag ccgaatgcgc cctcgtcggc
     1321 ctcgtccggc gtgccgccgc ctccgtagga ataggtgcgg tagaacggag actgcccgcc
     1381 cggacggctc tggttgcgcg gctcttcgcc cgtgaacccg gcgtcgcgat aggcgcgcca
     1441 ttcgtcgccg taccggtcgt agagcttgcg gtccttctga tcgctcagga cctcatacgc
     1501 ttcattgact cgcttgaact gctcttcggc atcgggattg tcgcggttgg catcggggtg
     1561 gtacttacgg gccagctttc gaaaggcagc cttcagctcc ttatcggtcg cggtgcgaga
     1621 aacgccaagc gtttcgtagt ggtctttgag ttgagatgac gccacggtcg atccctcatt
     1681 attcattgac catataataa acgttgagtc ttaccgacgc aaggtgtgcc ctgatctggc
     1741 gcgggacagg gtcgccttgc cgaagttcgc cccccaacga tacgtcgcgc ggaccagcag
     1801 ccagttgagg aaggctgcaa ttggccccag gatgccgaac gccactgtgc agacggccgt
     1861 gatgatcgat gcgaagtcct caactacgct gacgaacggc gttccgaggc cgatcccctc
     1921 acgcgcgagg tggatccggc gctggacctt gtcccagtgc acgaggccgc cgatgaccag
     1981 gccgaagacc atggcgagga tggggtccat cgacgagtcg tgatcggtga tcgccatgaa
     2041 acaaagcgcg ccggtggcgg gccgcaggac cgtcccgagc acatcgagaa tgtgatccag
     2101 gcgcggcacc ttatcggcca ccagctccac ggtcatgagg gccaggaaaa cgaggatgct
     2161 gccgacagag gagatgaagt caaacggccg actgaggagg tcgccgccag tgatccggtc
     2221 cgccagtgcc aaaataagga gcggcgtgaa ggtgttccaa ccgcacgccg ctgccagccc
     2281 aatgccggcg aggattcctg agttcaagag cgcttcctga cgtgcatgag tcgatcgatc
     2341 gaatgccgtc tggaatcatg ccgcatcctg aggagcggcg tcgatcagcg cttacgccat
     2401 cggccggatc accgtgacca gcttgccctg gatttcgaca ttgcccgggt aggtgtagat
     2461 cggctccatc gtggcattcg ccggctgcaa ccggatccgg ctgccctcgt gatagaactt
     2521 cttcagcgtc gtctcgcgct catccttcag ccagacagcg acggtctcgc cgttctcgca
     2581 cgtcgcctgc tggcgaagga gaacgatatc gccgtcgttg atcagggcat cgaccatcga
     2641 gaggcccttg acccg
//
LOCUS       DASSGX010000467         2583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1060764, whole genome shotgun sequence.
ACCESSION   DASSGX010000467 DASSGX010000000
VERSION     DASSGX010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1060764"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /locus_tag="VFI12_08375"
     CDS             complement(<1..1002)
                     /locus_tag="VFI12_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HET7056465.1"
                     /translation="MDENLGAVQLASIETEMRSSYLDYAMSVIVQRALPDARDGLKPV
                     QRRILYAMSELGMRSNSRYRKSAGVVGEVLKNFHPHSDTAVYDTLVRMAQNFNLRYPL
                     IDPQGNFGSVDGDGAAAMRYTECRLAPIADEMLSDIDKNTVDFKPNYDASTKEPKVLP
                     ATLPNLLVNGAVGIAVGMATNIPPHNLTEIADAVRYVIDNPDCTIEDLMEIVQGPDFP
                     TGGTILGREGIRAAYSTGRGRVVIRAKAFVEESARGNRFQIVATELPYQVNKAALLER
                     IAEMVKDGKIDGISDLRDESDRTGMRAIIELKRDAQPTKVLNNLFKHTQLQSSFGVNM
                     "
     gene            complement(1160..2353)
                     /locus_tag="VFI12_08380"
     CDS             complement(1160..2353)
                     /locus_tag="VFI12_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HET7056466.1"
                     /translation="MAEGGNGSNGQHANGTTGHSKREDGKVRVAIIGVGNCASSLVQG
                     VQYYKDAPDDEFVPGLMHVNLGGYHIRDIEFTAAFDIDAEKVGKDLSEAIFSGPNNTM
                     KFADVPNLGIKVERGMTHDGLGKYLSQVITKAPGATANVAKILEDTQTDVVISYLPVG
                     SEMATKWYVEQVLEAGCGFVNCIPVFIASQEYWANRFKERGVPIIGDDIKSQVGATIT
                     HRVLTRLFADRGVRIDRTYQLNFGGNTDFLNMLERERLDSKKISKTNAVTSQLDYAMD
                     ARNVHVGPSDYVPWLEDRKWCHIRMEGTTFGDVPLNMELKLEVWDSPNSAGVVIDAVR
                     CAKLAMNNGISGQLLAPSSYFMKSPPVQYHDDDARDQVEEFIKKNGRKKIAAKQPAKI
                     EAEAV"
BASE COUNT          427 a          774 c          841 g          541 t
ORIGIN      
        1 catgttcacg ccgaaactgg attgcagttg cgtgtgcttg aacaggttgt tcagcacctt
       61 ggtcggttgc gcgtcacgct tgagctcgat gatggcgcgc attccggtgc ggtccgactc
      121 gtcgcgcagg tcggagatac cgtcgatctt gccgtccttg accatctcgg caatccgctc
      181 gagcagggcg gccttgttga cctggtaggg cagttcggtg gcgacgatct ggaagcgatt
      241 gccgcgagcc gattcctcga cgaacgcctt ggcccggatc acgacgcggc cgcggccggt
      301 cgaatacgcc gcccggatgc cctcgcgccc gaggatcgtg ccgccggtcg ggaaatccgg
      361 tccctgcacg atctccatca ggtcctcgat cgtgcaatca gggttgtcga tcacgtatcg
      421 gacggcgtcg gcgatttcgg tgaggttgtg tggcgggata ttggtcgcca tgccgaccgc
      481 gatgccgacc gcgccgttga ccagcaagtt cggcagcgtg gccgggagca ccttcggctc
      541 cttcgtgctg gcatcgtaat tgggcttgaa atcgaccgtg ttcttgtcga tgtcgctgag
      601 catctcgtcc gcgatcggcg cgaggcgaca ctcggtgtat cgcatggccg ctgcgccgtc
      661 gccgtcaacc gagccgaagt tgccctgcgg gtcgatcagc gggtagcgca ggttgaaatt
      721 ctgggccatg cggacgagcg tgtcgtagac ggccgtgtcg ctgtgcgggt ggaagttctt
      781 gagaacttca ccgacaacgc cggcgctctt gcggtagcgc gagttgctgc gcatcccgag
      841 ctcggacatc gcataaagga tgcggcgctg gaccggcttg aggccgtccc gggcatccgg
      901 cagagcgcgc tgcacgatga cgctcatcgc gtaatcgagg tagctgctcc gcatctcagt
      961 ttcaatgctg gcgagttgta ctgcgccgag attctcgtcc aatgtagggg gttccttgca
     1021 tgctgaaacg cgaatgctta ccgcaatagt ttaccagatt atgggcctct ccggccacct
     1081 gaaaacccgc gtacggacgc ggaaaaggca cgaaaaaggg ccggcaaagt tgccggcccc
     1141 ggttggtgat ttcgagctgc tagacggctt cggcttcgat cttggccggt tgcttcgccg
     1201 cgatcttctt gcggccgttc ttcttgatga actcctccac ctggtcccgt gcatcatcgt
     1261 cgtgatattg gaccggcggc gacttcatga agtaggagct cggcgccagc aattgcccgc
     1321 tgatgccgtt attcatggcc agcttggcgc agcggacggc gtcgatgacg acgccggccg
     1381 agttcgggga atcccagacc tcgagtttaa gctccatgtt gagcgggaca tctccgaagg
     1441 tcgtgccttc catacggatg tggcaccact tgcggtcctc gagccacggg acgtaatccg
     1501 acgggccgac gtggacgttc ctggcgtcca tcgcgtaatc gagctgcgac gtgacggcgt
     1561 tcgtcttcga aatctttttc gagtcgagcc gctcccgttc gagcatgttc aggaagtcgg
     1621 tattgccgcc gaagttgagc tggtaggtgc ggtcgatccg cacgccgcga tcggcgaaga
     1681 ggcgggtcag gacgcggtgc gtgatcgtcg cgccgacctg gctcttgatg tcgtcgccga
     1741 tgatcgggac gccgcgctcc ttgaaccggt tcgcccagta ctcctggctg gcaatgaaga
     1801 ccgggatgca attgacaaag ccgcaaccgg cctcgagcac ctgctcgaca taccacttgg
     1861 tcgccatctc ggagccgacc ggcaaatagg agatgaccac gtcggtctgg gtgtcctcga
     1921 ggatcttggc gacgttggcg gtcgccccgg gcgccttggt gatgacctgc gagaggtatt
     1981 tgccgagacc gtcgtgagtc atgccgcgct cgaccttgat gcccaggttc ggcacgtcgg
     2041 cgaatttcat ggtgttgttg gggccgctga agatggcctc gctcaggtcc ttgccgacct
     2101 tctcggcatc gatgtcgaag gcagcggtga actcgatgtc gcggatgtga taaccgccaa
     2161 ggttgacgtg catcagcccg ggcacgaact cgtcgtccgg cgcgtccttg tagtactgca
     2221 cgccctggac cagtgacgag gcgcaattgc cgacgccgat gatcgcgacg cggaccttgc
     2281 cgtcctcccg cttcgaatgg cccgtagtgc cattcgcatg ctgaccgtta ctgccgttcc
     2341 caccttctgc cacgtgtgtt gctcctgaga agatcgtgac gtccagcccg cacggggacg
     2401 tccggccgag tgagccgccg gaagtctagg cggggcatga atggggcgtc aacgcttcct
     2461 ttcgggtagt agcgtttacg gcggctattg tgctgaggcc accggcatgc cgcaattcgg
     2521 gcagaacttg gcgcccggag gaagcgcctt gccgcattcc acgcagaagg cggcgccggc
     2581 cgg
//
LOCUS       DASSGX010000468         6780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1171606, whole genome shotgun sequence.
ACCESSION   DASSGX010000468 DASSGX010000000
VERSION     DASSGX010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6780
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171606"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="VFI12_08385"
     CDS             <1..993
                     /locus_tag="VFI12_08385"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7056467.1"
                     /translation="NNRLQLDKFEDYWDTKSVADQFVFSAALDETAAIEQLRAGDVDF
                     YENVPAADVESLKQEPDLNVAIYPTYSFTWWGYNLDPEKTPLFQDVEVRQALLYAVDR
                     QTMVDTLLLGFGEVANGTQPTLSPAYAPDRIDTVYSYDPDKAKQLLAAAGWTDTDGDG
                     IVDKDGQKLSFQIMYGSGSSATDQVVAAIQDYWKAVGVDGQPNPVDFGQVLVPALTSN
                     FNFQLCLLGFQWDPTGDQSAMFSTDAYGAGFNAMKYSNKEVDKLYAEAGRELDDKKRI
                     DLLVQATNLVNDDLPVAVLWFRSDRTGYNKRMHNFAPNAQAGLLWSIPYVWVES"
     gene            1067..2029
                     /locus_tag="VFI12_08390"
     CDS             1067..2029
                     /locus_tag="VFI12_08390"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7056468.1"
                     /translation="MASYVIRRLLIMPVILFCVALVTFVIINLKGNPVDRFAFNPRVK
                     PEDIARMKHNLGLDKPVGERFFLYIQSLVRGDLQNSLINSKPVTVLIGDALPNTLRLA
                     IVSIILAFVIATPIAIYAATHRNSAADRFILTTAVAGSSIPTVWLGLLLIIVFAVKFR
                     EWGFPYLPINGVKDTRNPGGYKDLAEHMILPVTALAIPQIAGWMWYIRNAMLEVLSQD
                     YIRTAQSLGLRDRTVRYVHAFRNCLTPLVTLAGLSIPDIFAGALITETVFGYPGMGYL
                     TVTAIGQNDYTVIMGTTLIYALLLVIGNLMADLLYPIVDPRLRG"
     gene            2038..2973
                     /locus_tag="VFI12_08395"
     CDS             2038..2973
                     /locus_tag="VFI12_08395"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7056469.1"
                     /translation="MATAAQTARQARAGTLEEVQILQRAPTGFWRDTWRRFRSDRGAM
                     FFGTIFVLILIFVLSGPLISRYITHFNYSENHLTQKLSTPGENSYILGSDGNGRDVLT
                     RLSYGGRISLLFALAAMISVLVLGGLIGGSAGFFGGFYDSVMMRIAEIVLAMPLLPLL
                     ILISAMYRPPVWQLAMFVGAFSWPGISRLIRGEVMSIRRREYIEAARVTGASNSRLLF
                     RHVFPNVLPIIIVWASLSVPSLILLEAILSYLGVGVRPPTPSWGNMLEEAQQFYRTAW
                     WLIFIPGFAIYITVLSMTLTGNGIRDAIDVRLQRK"
     gene            3063..4049
                     /locus_tag="VFI12_08400"
     CDS             3063..4049
                     /locus_tag="VFI12_08400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HET7056470.1"
                     /translation="MQVSTGQLIRGLIVGLVLALVVLTGLTVIGDARKLRDTISQFQW
                     IYLAPIVALTIWNYWWRFRKWEMYLEALGVPHIPRSTGLPIFLSGFSMSVTPGKVGEV
                     IKAFFVRRETNAAVERVTGAVVAERGTDALAMLVLALIGATQFSYGRLFVGAFVVLAA
                     IALYVLRQPRILGALVRLLARVPVLNRVAHLAESFFEATTHLLSPALLVRGTALGVVS
                     WTGECVAFFLVLAGLGLPATWSLLMIAVFVLAVSSLAGGISMLPGGLGVADASVAGML
                     LVLVKDPQMTDTVAITATLLIRFATLWFAVLIGAIMLLVLRRKSRVAPLGTA"
     gene            4374..4547
                     /locus_tag="VFI12_08405"
     CDS             4374..4547
                     /locus_tag="VFI12_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056471.1"
                     /translation="MMPPEEPRRRRWLWVTLGLILGCIILCVVFFAAAGRSDTVADFM
                     TSVADFQTEEAGN"
     gene            complement(4616..>6780)
                     /gene="pnp"
                     /locus_tag="VFI12_08410"
     CDS             complement(4616..>6780)
                     /gene="pnp"
                     /locus_tag="VFI12_08410"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HET7056472.1"
                     /translation="IPGGFIKREGRPTELAILAARLTDRPIRPLFPKGYMAEVQVVST
                     VMSADQENDPAILSIIGASAALSISPIPWEGPVGAVRVGRVDGHVVINPTFSELENSD
                     LDMVVAGTAEAIVMVEGEAHEISEEQLLEAIMGAHDEIKRICEVQNDLVTAAGMEKWE
                     FVAPAKDEALLDDVRSFLGTKLSDRVNNPDKVMRLEGTNELELELREHFTTAPDGQEP
                     KYTVAQVSGAFDSLLKETVRGAILASGTRPDGRTPTEIRPIWSRVGYLPRTHGSAVFT
                     RGQTQVISVATLGTESDAQRLDSISPITTKRYIHHYNFPPYSVGEIKFMRGPSRRDIG
                     HGALAERALLAVLPSEEEFPYAIRVVSDVVSSNGSSSMASVCGSTMSLMDAGVPIRKP
                     VAGVAMGLVTDDSGHYTVLTDIQGLEDHLGDMDFKVAGTDDGVTAIQMDIKVKGLTAD
                     ILRQALSQAHDGRIFILGKMLETISEPRADISPFAPRITQIKINPDKIGLLIGPGGKN
                     IRALQEETGAKIDIADDGTVSIASVDKTSTDAAIQKIKGMTDEIKIEVGEIYTGKIVG
                     IQTYGAFVQLMPGKDGLVHISELSEDPAVRVNKVEDLFNLGDEITVMVTEVAPNGKVS
                     LSRRAAITGEMPAPKSNDRGPRGGGGDRGPRRDGGDRGPRRDGDRDRDRGPRHEGDRG
                     PRHDGDQNRAHRDESDAEVGTRRDSDRREGDNRGRPAW"
BASE COUNT         1110 a         2207 c         2090 g         1373 t
ORIGIN      
        1 aacaaccggc tccagctcga caagttcgag gattactggg acacgaagtc ggtcgccgac
       61 cagttcgtgt tctcggccgc gctcgatgag accgccgcga tcgagcagct ccgtgctggc
      121 gatgtggact tctacgagaa cgtcccggcc gccgatgtcg agagcctgaa gcaggagccg
      181 gatctcaacg tcgcgatcta tccgacctac agcttcacgt ggtggggcta caaccttgac
      241 ccggagaaga cgccgctctt ccaggacgtc gaggttcgcc aggcgctcct gtatgcagtc
      301 gatcgccaga cgatggtcga tactctgctg ctcggcttcg gcgaggtcgc caacggcacg
      361 cagccgaccc tctcaccggc gtatgccccg gatcggatcg acaccgtcta cagctacgac
      421 ccggataagg cgaagcagct cctcgcggct gccggttgga ccgatacgga cggtgacggc
      481 atcgtcgaca aggatggcca gaagctgtcc ttccagatca tgtacggctc cggctcctcc
      541 gcgaccgacc aggtcgtggc cgctatccag gactactgga aggccgtggg tgtcgatggc
      601 cagccgaacc cggtcgactt cggccaggtg ctcgtgccgg cgctgaccag caacttcaac
      661 ttccagctct gcctcctcgg cttccagtgg gacccgaccg gcgaccagtc ggcgatgttc
      721 agcacggacg cctatggcgc gggcttcaac gccatgaagt actccaacaa ggaagtcgac
      781 aagctgtacg ccgaagccgg ccgcgagctg gacgacaaga agcggatcga tctcctggtc
      841 caggcgacca acctggtcaa cgacgatctg ccggtggctg tgctctggtt ccgcagcgac
      901 cggaccggct acaacaagcg catgcacaac ttcgctccga acgcgcaggc cggcctcctc
      961 tggtcgatcc cgtacgtctg ggtcgagtcc taaatcgaac gtcgtattcc ggggggcagc
     1021 ttcggctgtc ccccggcgtt tcttaccgac gagcgggcat tgagtcatgg ccagttacgt
     1081 aatccgccgc ctgctgatca tgccagtgat cctcttctgc gtggcactgg tcaccttcgt
     1141 gatcatcaat ctcaagggca acccagtcga ccggttcgcg ttcaatccac gcgtcaagcc
     1201 ggaagacatc gcccgcatga agcacaatct cggcctggat aagccggtcg gggagcgctt
     1261 cttcctctac atccaatcgc tggtcagggg cgacttacag aattcgctga tcaattcgaa
     1321 acccgtcacc gtcctaatcg gtgacgcttt gccgaacaca cttcgacttg cgatcgtgtc
     1381 gatcatcctg gccttcgtga tcgcgactcc cattgccatc tacgcggcga cgcaccgcaa
     1441 ctcagcggct gaccggttca tcctgaccac ggcggttgcg ggatcatcga ttccgaccgt
     1501 ctggctcggc ctgctgctga tcatcgtctt cgcggtcaag ttccgcgagt ggggctttcc
     1561 gtacctgcca atcaacggag tgaaggacac tcgaaacccc ggcggataca aggacctggc
     1621 cgagcacatg atcctgccgg ttacggcgct ggccataccc cagattgcgg gctggatgtg
     1681 gtacatccgg aatgccatgc tcgaggtgtt gagccaggat tacatccgca ccgcccagtc
     1741 gctgggtctg cgcgatcgca cggttcggta tgtgcacgcg tttcgcaact gcttgactcc
     1801 gctcgtgacg cttgccggtc tttcgattcc cgacatcttc gccggcgcgc tgattaccga
     1861 aacagtattt ggttatcccg gcatgggtta tttgacggtc accgcaatcg ggcaaaacga
     1921 ctacaccgtc atcatgggta cgaccctaat ctatgcactg ctcctcgtta tcggaaacct
     1981 gatggccgac ctcctttatc cgatcgtaga cccacggctg cggggatagg acgcgatatg
     2041 gcaacggcgg cacaaactgc ccggcaggct cgcgcgggca ctcttgaaga agtccagatt
     2101 ctccaacggg cgccaaccgg cttctggcgc gacacgtggc gacgcttccg gtcggaccgg
     2161 ggtgcgatgt tcttcgggac gatcttcgtg ctgatcctga tcttcgtcct gtcgggcccg
     2221 ctcatctccc ggtacatcac gcatttcaac tactcggaaa atcacctcac gcagaagctc
     2281 tctacgcccg gcgagaacag ctacatcctg ggttccgacg gaaatggccg ggatgtgctg
     2341 acccggctct cgtacggcgg gcgcatctcg ctgctctttg cgctggccgc catgatctcg
     2401 gtcctggttc tcggcggtct catcggcgga agcgcggggt tcttcggcgg gttctacgac
     2461 tcggtcatga tgcgaatcgc cgaaatcgtg ctcgcgatgc cgttgctgcc gttgctcatc
     2521 ctcatctcgg cgatgtaccg gccaccagtc tggcagctgg cgatgttcgt cggtgcattc
     2581 agttggccag gcatatcccg cctgatccgc ggcgaggtga tgtctatccg aaggcgggaa
     2641 tacatagaag cagcgagagt aaccggggca tcgaactcgc gcttgctctt cagacacgtc
     2701 ttcccgaacg tcctgccgat catcatcgtc tgggcgtcgc tttccgtccc gagcctgatc
     2761 ctgctcgagg cgattctcag ctacctcggc gtcggcgttc gtccgcctac cccgtcgtgg
     2821 ggcaacatgc tcgaggaggc ccagcagttc taccgcacag cctggtggct gatcttcatt
     2881 cccggtttcg cgatctacat cactgtgctc tcgatgaccc tgaccggcaa cggcattcgc
     2941 gacgcgatcg acgtgcggtt gcagcgcaag tagaaccaat cgatcattag ccactgcggc
     3001 ccgcttccaa atgccggatt cccgactccc cgcggcgggc ccttttcgtg ccctggagtt
     3061 ccatgcaggt ttcgactgga caactgatcc gcgggctcat cgtcggcctc gtgctggcgc
     3121 tcgtcgtact gaccggactg acggtgatcg gcgacgcccg caagttgcgg gacacgatct
     3181 cgcagttcca gtggatctac ctcgcgccga ttgtcgcgct cacgatctgg aactactggt
     3241 ggcggttccg caaatgggag atgtacctcg aggcgctggg tgtgccgcac atcccccgat
     3301 ccacgggcct gcctatcttt ctgtccgggt tttcgatgag tgtgacgccc ggcaaggtcg
     3361 gcgaggtgat caaggcgttc ttcgtccggc gtgagacgaa tgcagcggtc gagcgcgtga
     3421 ccggcgcagt cgtcgcggag cgtggcaccg acgctctcgc gatgttggtc cttgcgctga
     3481 tcggggcgac gcagttcagt tatgggcggc tgttcgtggg cgcgttcgtc gttctcgccg
     3541 ccattgcgtt gtacgtgctc aggcagccgc gaatccttgg cgcactcgtg cgcctgctgg
     3601 cccgcgtgcc ggtcctgaac cgggtagcgc acctcgccga gtcgttcttc gaagcgacga
     3661 cacatctgct ctctccggcg ctgctcgtcc gcgggacggc gctcggggtc gtctcctgga
     3721 cgggcgaatg cgtcgcgttc tttctggtcc tggccgggct gggacttccc gcgacctggt
     3781 ccctgctgat gatcgcggtc ttcgtccttg ccgtttcatc gctcgcgggc gggatctcga
     3841 tgctgccggg cggactgggg gtggcggatg cgagcgttgc cggcatgctg ctcgtattgg
     3901 tcaaagaccc ccaaatgacc gacaccgtgg cgattacggc caccttgctg atccgatttg
     3961 ctaccctttg gttcgctgtc ctgatcggag caatcatgct tctggttctg cggcggaaat
     4021 cgcgggtcgc gccactgggg actgcataga acacctgacg atgtaaggag ggtccagtga
     4081 tcaagtgtcc caactgcggg accatgaatg agcccgggag ccgattctgc taccagtgcg
     4141 gaacggattt gcgcggcgtc ccaccggcag agccgccgca gcctgaacag caaccaatcg
     4201 ggcagcgcgg cacgttacca ccgccgcccg gcttcccgtc tgctccgcag ccgccgggct
     4261 ttcctccgcc tccgccgccg tcgtcgggat ttccgccacc accgtcgccc tcaggttacc
     4321 cgccaccacc gccgggctat gacccggcac ggcagggatg ggccccgggg tacatgatgc
     4381 cgccggaaga gccacgtcgc cggcgctggc tgtgggtgac gctcggcctg atcctcggct
     4441 gcattatcct ctgcgtcgtc ttctttgccg ctgccggtcg ctcagacacc gtggcggatt
     4501 tcatgacctc tgtcgccgac ttccagaccg aggaagccgg gaactgatgt aacagaggca
     4561 acgaaaacgg gacggcagag aactgccgtc ccgttttgct tgtcatgatt ccgggctacc
     4621 aggcaggccg gccgcggttg tcgccctcac ggcgatcgct gtcccggcgc gtcccgactt
     4681 ccgcatcact ctcgtcgcgg tgagcccggt tttggtcgcc gtcgtggcgt ggtccccggt
     4741 ctccttcgtg gcgcggtcct cgatcgcgat cccggtcgcc gtcgcggcgc ggaccacgat
     4801 ccccgccgtc acggcgtggg ccgcgatcgc caccaccacc gcgtggaccg cggtcgttgc
     4861 tcttcggcgc cggcatctcg ccggtgatcg ccgcgcggcg ggacaggctg accttcccgt
     4921 tcggcgcgac ttccgtgacc atgacggtaa tctcatcgcc caggttgaac aggtcctcga
     4981 ccttgttcac ccgcaccgcc ggatcctcgg aaagctccga gatgtggacc aggccgtcct
     5041 tgcccggcat gagctggacg aacgcgccat aggtctggat accgacgatc ttgcccgtat
     5101 agatctcgcc gacttcgatc ttgatctcgt cagtcatgcc cttgatcttc tggatcgcgg
     5161 cgtccgtgct cgtcttgtcg accgaggcga tcgagaccgt gccgtcgtcg gcgatgtcga
     5221 tcttggcgcc ggtctcctcc tggagcgcgc ggatgttctt gccgcccggg ccgatcagca
     5281 agccgatctt gtccggattg atcttgatct gggtgattcg cggcgcgaat ggcgagatat
     5341 cggcccgcgg ctcggagatt gtttcgagca tcttgccgag gatgaatatc cggccgtcgt
     5401 gcgcctgcga gagcgcctga cgcaggatgt ccgccgtcag gcccttgacc ttgatatcca
     5461 tctggatcgc ggtcacgccg tcgtcggtgc cggcgacctt gaagtccatg tcgccgaggt
     5521 gatcctcgag accctggatg tcagtcagca cggtgtagtg gccggaatcg tccgtgacga
     5581 ggcccatcgc gacgcccgcg accggcttgc ggatcggcac gccggcgtcc atcaacgaca
     5641 tcgtgctgcc gcagacgctg gccatcgacg acgagccatt ggagctgacg acgtcggaga
     5701 cgacgcgaat tgcgtatggg aactcttcct cacttggaag gaccgccagc agcgcccgct
     5761 ccgcaagtgc gccatgaccg atgtcccggc gactcgggcc gcgcatgaac ttgatctcgc
     5821 caacgctgta cggcgggaag ttgtagtggt ggatgtagcg cttggtagtg atcgggctga
     5881 tcgaatcaag ccgctgcgca tccgattcgg tgccgagcgt cgcaaccgaa atcacctgcg
     5941 tctgtccgcg ggtgaagacc gccgagccat gggttcgcgg cagatatccc acgcgcgacc
     6001 agatcggtcg gatctcggtc ggggtgcggc catccgggcg agtcccgctg gcgaggatcg
     6061 cgccacggac cgtctccttg aggaggctgt cgaaggcgcc ggaaacctgc gcaacggtgt
     6121 atttcggctc ctgcccgtcg ggcgcggtcg tgaagtgctc gcgaagttca agctcgagct
     6181 cgttggtacc ctcgaggcgc atcaccttgt cggggttgtt gacccgatcg ctcagcttgg
     6241 tgccgaggaa ggagcggaca tcgtcgagca atgcctcgtc cttggccggc gcgacgaact
     6301 cccacttctc catgccggcg gccgtgacca ggtcgttctg cacttcgcag atgcgcttga
     6361 tctcatcgtg ggcgcccatg atcgcctcga gcagctgctc ctcggatatt tcgtgggcct
     6421 caccctcgac catgacgatg gcctcggcag tgccggccac caccatgtcg agatcgctgt
     6481 tctcgagctc gctgaaggtc gggttgatca cgacgtggcc gtcgactcga ccgacgcgta
     6541 ccgcgccgac cgggccttcc cacgggatcg gcgaaattga aagcgctgcc gaggcgccga
     6601 tgatcgagag gatcgccggg tcattctcct ggtcggcaga catcacggtc gagaccacct
     6661 ggacctcggc catgtagccc ttcgggaaga gtggccggat cgggcggtcg gtcaagcggg
     6721 cggctaggat tgccagttca gtcggccggc cctcgcgctt gatgaagccg cccggaatct
//
LOCUS       DASSGX010000469         2836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1279412, whole genome shotgun sequence.
ACCESSION   DASSGX010000469 DASSGX010000000
VERSION     DASSGX010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1279412"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="VFI12_08415"
     CDS             <1..802
                     /locus_tag="VFI12_08415"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HET7056473.1"
                     /translation="TILGSTIVFAPFDIAQALEGLAAAGYRNCEIGAVKGWFEHIDPD
                     TVTDKEIADIKQRLNDLDLHPVSLSAHTQLQTPEGKERFRRALVIANGLGMEIVNTIT
                     GDAETEADRETWFANMQDLCDEAEKLGLIIGMETDSNMLPTAAIGVAILDRVNRPGRI
                     GFNYDPGNVVYYAGADPIEDIKIALPRMVHFHFKDKVSGKGVFNFPPPGEGELDMPAM
                     LELCAEAGYDGPISAEVEFDENGWPDYDACLEAAKRSVANLHAMGLTW"
     gene            1117..2712
                     /locus_tag="VFI12_08420"
     CDS             1117..2712
                     /locus_tag="VFI12_08420"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HET7056474.1"
                     /translation="MPIDYDRLAERLAAMKTSRRNLGIGAAGAVMSLGGVALLGTKAL
                     GNEGSPPEPTQPALAIQDPQPTAVAGVANAPDSAPAVIRSAPQAAVPDGMALVSSPRL
                     PLFDLASQDVGALVSGKVPSWIEVGSALDLAVSPVAINGATMDGLQATDTFADYKALA
                     DYLNSADGIGGVALVPADQVDARVNVLAVDGYDPVRDGEFGEPTIRIGFVGDIVPGRN
                     VGIKMREYNDYTHPFHRVAAILSSYDLTIANLEGDLSDDLPVPTEGATFDFVASTAMI
                     DGLEMSGIDAVSQANNHSTWNSAGWGTQGLTDTIDALDARGFGHFGGGRTLEQARAPY
                     TTTVAGKSIAIIGIDGVTANVEAREPDATVNNSWLGGEKYAGATNDTPGTYPYDPEIF
                     LPDIQTLAGQYDIVIPYFHFGREYIEAPPDWAVDGARSAIDAGATLVVTNHPHVIQGM
                     EFYSGTPILYSVGNFIFDQLFSVQTRQGLILELVLRGGTCVGLRTRGVEIEDFNQPRL
                     MNAGEQAAIMDRFWASTDRLATR"
BASE COUNT          540 a          965 c          883 g          448 t
ORIGIN      
        1 gaccatcctc ggctcgacca tcgtcttcgc gccgttcgac attgcgcagg cgctcgaggg
       61 gctggccgcg gccggctatc gcaactgcga gatcggcgcg gtcaagggct ggttcgagca
      121 catcgatccc gacaccgtga ctgataagga gatcgccgac atcaagcagc ggctgaacga
      181 ccttgacctg cacccggtca gcctcagcgc ccacacccag ctccagacgc ctgagggaaa
      241 ggagcgcttc cgccgcgctc tggtcatcgc caatggcctc gggatggaga tcgtcaacac
      301 catcaccggc gatgccgaaa ctgaggccga tcgcgaaacc tggtttgcca acatgcagga
      361 cctctgcgac gaggccgaga aactcggcct gatcatcggc atggagaccg acagcaacat
      421 gcttccgacg gcggcgatcg gcgtcgcgat cctcgatagg gtcaaccgcc cgggccggat
      481 cggcttcaac tacgacccgg gcaacgtcgt ctattacgcc ggcgccgacc cgatcgagga
      541 catcaagatt gcgctgcccc ggatggtcca tttccacttc aaggacaagg tcagcggcaa
      601 gggcgtcttc aacttcccgc cgcccggcga aggcgagttg gacatgccgg ccatgctcga
      661 gctctgcgcc gaggccggct acgacgggcc gatcagcgcc gaggtcgagt tcgacgagaa
      721 cggctggccc gactacgacg cctgcctcga agcggccaag cgctccgtcg ccaacctgca
      781 cgcgatgggc ctgacctggt aagcgagcag tcgaggaggc gagcaggcga gaaggcgaag
      841 gcgcggaatg gtaggggcag atggcatctg ccctccgctc ccgaccgggc cggccatatg
      901 cggtcgggcg tgcccggcgt cgcccgggcg taaatacccg ggctgaatcc aataagcccg
      961 ctgaagcagg ctggaccagg aatcgggggc gggggacgat acgctcttct ccgaaaaccg
     1021 aaaaccgaaa accgaaaacc gaaaaccaaa gaccttcatc acgcggtaca ctcaccggca
     1081 tgacgtcacc gctatcaatc aggattgcgc cggcccatgc cgattgatta cgatcgcctg
     1141 gccgagcgcc tggcagccat gaagaccagc cgccggaatc tcgggatcgg cgcagccggc
     1201 gcagtcatgt cgctcggcgg cgtggcactc cttggcacga aagcgctggg caacgaaggc
     1261 agtccgcccg aaccgacaca accagccctc gccatccagg atccgcaacc gaccgcggtc
     1321 gccggcgtcg cgaacgcgcc cgattcggct ccggcagtca tccggtctgc gcctcaggcg
     1381 gcggtcccgg atgggatggc gctcgtttcg tcgccccgct tgccgctttt cgacctcgcc
     1441 tcgcaagacg tcggcgcgct ggtcagcggt aaggtgccat cgtggatcga ggtcggctcc
     1501 gcgctcgacc ttgccgtctc gccggtcgcc atcaacggcg cgacaatgga cgggctgcag
     1561 gcgaccgata cctttgccga ttacaaggca ttggccgatt acctgaactc tgcggacggc
     1621 atcggcggcg tcgcacttgt cccggccgac caggtcgatg cccgggtcaa tgtgctcgcg
     1681 gttgatggct acgaccctgt tcgcgacggc gagttcggcg agccgacgat ccggatcggc
     1741 ttcgtgggcg acatcgtccc cggccgcaac gtcggcatca agatgcgcga gtacaacgac
     1801 tacacgcacc cgttccaccg ggtcgccgcg atcctcagct catacgacct gacgatcgcc
     1861 aacctcgagg gcgatctctc cgacgatctc ccggtgccga ccgagggcgc gacattcgac
     1921 ttcgtggcca gcaccgccat gatcgacggc ctcgagatgt ccgggatcga cgcggtcagc
     1981 caggcgaata accacagcac ctggaacagc gccggctggg gcacgcaggg cctgaccgac
     2041 acgattgacg cgctcgacgc gcgcggtttc ggtcatttcg gtggcggccg cacgcttgag
     2101 caggcccgcg cgccctacac cacgacggta gccggcaagt cgatcgcgat catcgggatc
     2161 gacggcgtca cggctaatgt tgaggcgcgc gagcccgatg cgacggtcaa caattcatgg
     2221 ctcggcggcg agaaatacgc cggcgcgacc aacgacacac cggggaccta tccctacgac
     2281 ccggagattt tcctgccgga tatccaaacc ctggccgggc agtacgacat cgtgatccca
     2341 tatttccact tcggacggga gtacatcgag gcgccgccgg actgggcggt cgacggcgcg
     2401 cggtctgcca tcgatgccgg cgcgacactg gtcgtgacca atcatcccca cgtcatccag
     2461 gggatggagt tctacagtgg cacgccgatc ctctattccg tcggcaactt catcttcgac
     2521 cagcttttca gcgtccagac tcgccagggc ctgatcctcg agctggtcct gcgcggcggc
     2581 acgtgcgtcg gcctgcgcac ccgtggcgtc gagattgagg acttcaacca gccccgcctg
     2641 atgaatgccg gcgagcaagc cgcgatcatg gaccgcttct gggcctccac cgaccgcctc
     2701 gcgacccgct gagtcctctc aacgatcaat cgagcgcgag gacccgtacc gtagcgtagc
     2761 gcggggttac catgccccgc tgtggccgca ggccacgata gcgttggcga catcgcttct
     2821 aaaatgaaat tcagtt
//
LOCUS       DASSGX010000470         2074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1500314, whole genome shotgun sequence.
ACCESSION   DASSGX010000470 DASSGX010000000
VERSION     DASSGX010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1500314"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="VFI12_08425"
     CDS             <1..670
                     /locus_tag="VFI12_08425"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922180.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HET7056475.1"
                     /translation="LLCIDNIFRFVQAGSEVSALLGRMPSAVGYQPTLAAEMGQLQER
                     ITSTKTGSITSVQAVYVPADDYTDPAPATTFAHLDATISLERSIAERGIYPAVDPLAS
                     TSRILDPNIVGDEHYRVAREVQQVLQRYRDLQDIIAILGIEELSEEDKQVVARARRIE
                     RFFSQPFFTAEQFTGTPGRYVTREETVRAFKEILEGKYDALPESAFMYKGGIDEVIAS
                     AQGQ"
     gene            767..1225
                     /locus_tag="VFI12_08430"
     CDS             767..1225
                     /locus_tag="VFI12_08430"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HET7056476.1"
                     /translation="MAKLDVEIVTGERVVLSETDVDMVVAPGSDGALGILPHHAPLIS
                     TLAAGELRVKKGNKEQAIIVFGGFIEVTPEKVTVLADTAERIEEIDVHRAEEARRRAE
                     DAIKNRGETVEIEDAMAALRRANLRLNVGRRRRRGGPGGTPMGAGPDNPV"
     gene            1325..>2074
                     /locus_tag="VFI12_08435"
     CDS             1325..>2074
                     /locus_tag="VFI12_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056477.1"
                     /translation="MVDVAVHTGMTPDLPPGNPPARALAIEGGVPLRGSVHIGGAKNA
                     ALPLLAATLLTADECTLTNVPDLSDIHTMVDLLRALGADVEFDTRMHRVRVRAADITT
                     TSAQPDLVAKMRASFLVAGPLLARFNETTASTPGGCKLGARPVDVDVRGFRKMGAEVH
                     ATDQMITARTVGLHGARIYMDYPSHTGTENLLMAAVLAVGTTTIVNAACEPEIVALGN
                     MLNRMGAQIAGLGSPTITIQGVDRLHGVSDMI"
BASE COUNT          371 a          700 c          657 g          346 t
ORIGIN      
        1 tctgctctgt atcgacaaca tcttccgctt cgtgcaggcg ggctcggaag tctccgcgct
       61 gctcggccgc atgccgagcg ccgtgggcta ccagccgacc ctggcggccg agatgggcca
      121 gctccaggag cggatcacct cgaccaagac cggttcgatc acttccgtgc aagccgtcta
      181 cgtgccggcg gacgactaca ccgacccggc accggccacg accttcgccc acctcgacgc
      241 gaccatctcg ctcgagcggt cgatcgccga gcgcggcatc tatccggcgg tggacccgct
      301 ggcctcgacc tcgcgcatcc tcgatccaaa tatcgtcggt gacgagcact atcgcgtggc
      361 ccgcgaggtc cagcaggtgc tgcagcgata tcgtgacctg caagacatca tcgccattct
      421 cggaatcgaa gagctctccg aggaggacaa gcaggtcgtg gcgcgggcgc gccggatcga
      481 gcgcttcttc tcgcagccgt tcttcacggc cgagcagttc accggcactc ccggccgcta
      541 cgtcacccgc gaggaaacgg tgcgcgcctt caaggagatc ctggagggta agtacgacgc
      601 ccttccggag tcggccttca tgtacaaggg cggcatcgac gaagtcattg cttccgccca
      661 gggacagtag tcatcgtcag gctattgcgg ggcgcttcgg tcccgcaata gccttctttt
      721 cgcccggtcg agcaacgagc tcggcacagc acgaggaacg gcatccatgg ccaagcttga
      781 cgtagagatc gtaacgggcg agcgtgtcgt cctctccgag acggatgtcg acatggtggt
      841 ggcgcccggt tcggatggcg cgctcggtat cctgccgcat cacgcgcccc tcatttccac
      901 ccttgcggcc ggcgagctcc gcgtcaagaa gggcaacaag gagcaggcga tcatcgtctt
      961 cggcggcttc atcgaagtga cgcctgagaa ggtaactgtc ctggccgaca ctgccgagcg
     1021 gatcgaggaa atcgacgttc accgcgcaga agaggcccgc cgccgggctg aggacgcgat
     1081 caagaaccgc ggcgagacag tcgagatcga agatgccatg gccgcgcttc gccgcgccaa
     1141 cctgcgcctg aacgtcggac gccggcgccg ccggggtggt ccggggggta cgccgatggg
     1201 cgctggtccc gacaatccgg tatagtcccc ccacaacccg accggcgacg atcatgtcga
     1261 ggatgagact gctccctcgt ccatcgggag cgctcccaca tggcatcgct caggagaagt
     1321 cactttggtc gatgtcgccg tgcacactgg catgactccc gatttgcccc caggtaatcc
     1381 accagcccgg gcgcttgcga ttgaaggcgg cgttccactt cgtggttcgg tgcatatcgg
     1441 cggagcgaag aatgccgcgc tgccgctgct cgccgcgacg ctgctgaccg ccgacgaatg
     1501 cacgttgacc aacgtgccgg atctctccga catccacacc atggtcgacc tgctgcgagc
     1561 gctcggcgcc gatgtcgagt tcgatacgcg gatgcaccgg gtccgggtcc gcgccgcgga
     1621 catcacgacg acatcagcgc agcctgatct cgtggccaag atgcgggcat cgtttctggt
     1681 cgccggtccg ctccttgccc gctttaacga gaccaccgcg tcgacgccgg gaggatgcaa
     1741 actcggcgcg cgcccggtcg acgtggacgt gcgcggcttc cgcaagatgg gcgccgaggt
     1801 ccacgcgacc gaccagatga ttaccgcgcg caccgttgga ctgcacggcg cgcgcattta
     1861 catggactac ccaagccaca cgggcaccga gaacctgctg atggcggcgg tgctcgccgt
     1921 cggcacgacc acgatcgtca acgcggcatg cgagccagag atcgtggcgt tggggaatat
     1981 gctcaaccgg atgggcgcgc aaatcgcggg gctcggctcg ccgaccatca ccatccaggg
     2041 tgtcgatcgc ctgcatggcg tctcggacat gatc
//
LOCUS       DASSGX010000471         4057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_190838, whole genome shotgun sequence.
ACCESSION   DASSGX010000471 DASSGX010000000
VERSION     DASSGX010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190838"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..1660
                     /locus_tag="VFI12_08440"
     CDS             50..1660
                     /locus_tag="VFI12_08440"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HET7056478.1"
                     /translation="MTQRDVWDGADLLNAFATATRLLTEQRATIDALNVYPVPDGDTG
                     TNMSLTMEAALNDARANSGPSPSVAVVAQRIAHGSLLGARGNSGVILSQVYRGFAQGV
                     AGLDKVDGRDLAHALRRASDTAYKAVMQPVEGTMLTVIRIAAEHAEQTAPRDPSLVAV
                     LRSALAGAREALANTPNLLEKLRQAGVVDSGGQGIVLVLEGFLATATGERPETGAIAA
                     ATSVAVADQDELEAFGYCTNFMITGQGIPFEQVRTELAAMGQSAVIVGDDTHVKVHLH
                     IENPGQALDYAIRFGALGQIKIDNMDTQVSARALQPAGLLPSAGIAVLAVASGAGIAE
                     AFRSVGVAGVVDGGQTMNPSVEQLVRAVNSAASTDVILLPNNSNLLMAAQHVPELAAK
                     SVRVVPTRTIAQGLAAAAAFRAGRPLDEVVTVMERAMRRIHSVEVSRADKDASVNGLR
                     VRRGQFIGIVDDHLDVASDDLLETATRSLERAVNPESELVTIFTGDAVDAETPHRLVQ
                     WVAERHQDVEVELVEGGQPHYQFVIAVE"
     gene            1665..2522
                     /locus_tag="VFI12_08445"
     CDS             1665..2522
                     /locus_tag="VFI12_08445"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET7056479.1"
                     /translation="MPGIALVCDSTADLTAADQQSLGVAVVPLNVHFGSEVVRDHVDI
                     SPGQFLDRLTSTTELPRTSQPSPAAFAAAFQDAAADHDAILVLALSSRLSGTYQSAVL
                     GAQTADLPIPIRVVDSLSASIGLGLQVRRARELIDRGRELDEIATALEGERGHYHLIF
                     FADTLEYLQRGGRIGKASQLLGSLLKIKPLLIVVDGEVEAYERTRSRSRAIEGLIEYA
                     RQFPAIVRVAILFDGTSPADVETITQGLADRVPESAIIAEQYGPIIATHVGPGALGLC
                     VFAEPGKTG"
     gene            2631..>4057
                     /locus_tag="VFI12_08450"
     CDS             2631..>4057
                     /locus_tag="VFI12_08450"
                     /inference="COORDINATES: protein motif:HMM:NF028500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET7056480.1"
                     /translation="MRSEWKAGATGRYLREIQAAIFGLNSLAPAQQTVEAITSIGNEL
                     RVYRSQPIPERQEQLKRTAERLKAIAPQLRPDDPPFGPIGTNPASLRAPKRTKPRPAA
                     VVRRPDEPVTVLGGVGTAVAAKLAQLGIATIDDLLRHTPRHHIDFSRPQAIGQVGPMF
                     GGDPNVTLAGRITELKTIPGKQRNRVEVRITDGTGWARITWFNPFVAKQLAVGDEIVV
                     HGPLDEFRGGVSLTGPEWELRDSAALARSRLIPVYRLTQGIGQKQLRRLTRQAIDETR
                     QTLLDPIPQSILAEHGLVGLPFAIDQRHYPANRELLADATRRLAFDELFYLQVGLIQR
                     KRAAEDLRGNSLADGAEAIPAFLDTLPFTLTGAQRRAIADVSADLGGSRVMQRLVQGD
                     VGSGKTVVAATAALQATRAGYQSAVMAPTEILAGQLRTTLGRLYEGLGDAAPAIELLT
                     GSTKKRDRTKLLERLVNSEVDVLVGT"
BASE COUNT          708 a         1367 c         1338 g          644 t
ORIGIN      
        1 tccgagtctc agggctccgg cacgagtcag cgggcaggac gccagagctg tgacccagag
       61 agatgtctgg gatggcgcag atctcctgaa cgccttcgcc accgccaccc gtttgctgac
      121 cgagcagcgg gccacgatcg atgcgctcaa tgtctaccca gtgcccgatg gcgacaccgg
      181 gacgaatatg agcctgacca tggaggcggc gctcaacgac gcgcgggcca acagcgggcc
      241 gagcccctct gtcgcagtcg tcgctcaacg catcgcccac ggctcgctgc ttggcgcgcg
      301 cggcaactca ggcgtcatcc tttcgcaggt ctatcggggt ttcgcgcagg gcgtggcggg
      361 tctcgacaag gtggacgggc gcgacctggc gcacgcgctc cggcgcgcgt ccgataccgc
      421 gtacaaggcc gtcatgcagc cggttgaggg tacgatgctg acggtcatcc gcatcgccgc
      481 cgagcatgcc gagcaaaccg cgccccgcga cccatcgctc gtcgccgtgc taagaagtgc
      541 actcgccggc gcccgagagg cgcttgccaa taccccgaat ttgctcgaaa agctgcgcca
      601 ggccggtgtc gtcgactccg gcggccaggg cattgtgctc gtcctggagg gcttcctggc
      661 cacggcgacg ggcgagcggc ctgaaactgg ggccatcgcg gccgccacat cggttgcagt
      721 ggccgaccag gatgaactcg aggccttcgg gtactgcacc aacttcatga tcacgggaca
      781 gggcattccc ttcgagcagg ttcggaccga gcttgcggcg atgggccaat ccgccgtgat
      841 cgtcggggac gacacgcacg tcaaggtgca tctccacatc gagaacccag ggcaagcgct
      901 cgactacgcc atccgcttcg gcgcgcttgg ccagatcaag atcgacaaca tggacacgca
      961 agtctcggct cgcgccttgc agccagcggg actcctgccg agcgccggga tcgctgtcct
     1021 ggccgtggcc agcggagcag gcattgccga ggcatttcgc tcggtcggag tcgccggcgt
     1081 cgtcgacggc ggccagacca tgaaccccag cgtcgagcag ctcgttcgcg cagtgaactc
     1141 cgccgcctca accgacgtga ttctgctgcc caacaacagc aatttgctga tggcagcgca
     1201 gcacgtaccg gaactggctg cgaaaagcgt gcgcgtcgtt cccacccgga cgatcgcgca
     1261 ggggcttgcc gcggcggcgg cgttccgcgc cggacgtccg ctggatgagg tcgtgaccgt
     1321 catggagcgg gcaatgcggc gcatccacag cgttgaagta tcccgggccg acaaggacgc
     1381 ttcggtcaac ggattgcggg tgcgtcgcgg ccaattcatc ggcatcgttg acgatcacct
     1441 cgatgtggcg tccgacgacc tgcttgaaac ggcgacacga tccctcgaac gcgcggtgaa
     1501 tccagagagc gagctggtga ccatcttcac cggcgatgcg gtcgatgcgg aaacgccgca
     1561 ccgccttgtg cagtgggtgg ccgagcgtca ccaggacgtt gaggtcgaac tcgttgaagg
     1621 cggccaaccg cattatcagt tcgtcatcgc ggtggagtag cggaatgcca gggatcgcgc
     1681 tggtctgtga cagcacggcc gacctgaccg cggcggatca acagtcgctc ggcgttgccg
     1741 tcgtgccact caacgtccat ttcggctcgg aggtcgtgcg cgatcatgtc gacatctcgc
     1801 cgggccaatt cctggaccgc ctgacgagca cgactgagct gccacgaacg tcgcagccat
     1861 caccggccgc gtttgcggcg gcctttcagg acgcggcagc cgaccacgac gcaatccttg
     1921 tcctggccct ctcgtccagg ttaagcggaa cgtaccagtc ggccgtcctc ggcgcgcaaa
     1981 ccgccgattt gccgatcccg atccgggtcg tcgattcgct gagcgcgtca atcggcctcg
     2041 ggttgcaggt acgccgggcg cgcgaactga tcgaccgggg ccgggagctc gacgagatcg
     2101 cgacggcgct ggaaggggag cgcgggcact accacctcat tttcttcgcc gacacactgg
     2161 agtacctgca gcgcggtggc cggatcggca aggcgagcca gctcctcggg tcgctgctca
     2221 agatcaagcc gctcctgatc gtcgtcgacg gcgaggtcga ggcgtatgag cgcacccgga
     2281 gccggtcccg cgcgatcgag ggcctgatcg agtacgcgcg gcagttcccg gcaatcgtcc
     2341 gcgtggcgat cctgtttgac ggcacatcac ccgcggatgt cgaaacgatc acgcagggac
     2401 ttgccgatcg cgtgcccgaa tcagccatca ttgccgagca gtacggcccg atcatcgcca
     2461 cccacgtcgg ccccggcgcg ctgggactct gcgtcttcgc ggaaccggga aagacgggct
     2521 gattcactat gccgcgcgtt cagtacactt gatctatggc tacgagccct cgatcgacat
     2581 ccgagcaatc ggaccgcgcc gactcgccgg tcgagcgagt cctggccctc atgcgctcag
     2641 aatggaaagc aggcgcgacc gggcggtatc tgcgcgagat tcaggcggcc attttcggcc
     2701 tgaactcgct cgcgcctgcc cagcaaaccg tcgaggcgat cacgtcgatc ggcaacgagt
     2761 tgcgcgtcta tcgcagccaa ccgatccccg agcgccagga gcagctcaaa cggactgccg
     2821 aacggttgaa ggccatcgcg ccgcagcttc gacccgatga tccaccgttt gggccgattg
     2881 gcaccaatcc cgcatccctg cgagcgccaa agcggacgaa gcccaggccc gcggccgtcg
     2941 ttcgccgccc cgatgagccg gtgacggtac tcggaggcgt cggcacggcc gtagccgcca
     3001 agctggcgca gctcggaatc gcgacgatcg acgatctctt gcgccacacg ccgcggcacc
     3061 acatcgactt ttcgcgcccg caggcaatcg gccaggtcgg gccgatgttc ggcggagatc
     3121 ccaatgtcac gctggcgggg cggattaccg agctcaagac catacccggg aagcagcgga
     3181 accgcgtcga agtgcggatc accgacggca ctggctgggc gcggatcacc tggttcaacc
     3241 cgttcgtcgc gaagcagctg gcggtcggcg acgagatcgt cgtccatggc ccgctcgacg
     3301 aatttcgcgg gggagtctcg ctgaccggac ctgagtggga gttgcgcgat tcggcggctc
     3361 tggcccgctc ccggctgatt ccggtctaca ggttgacgca ggggatcggc cagaagcagt
     3421 tgcggcggtt gactcggcag gcgatcgacg agacccggca gaccctgctc gatccaattc
     3481 cgcagtcgat cctggccgaa cacgggctgg ttgggctgcc cttcgcgatc gatcagcggc
     3541 attacccggc gaaccgggag ctgctcgccg atgcgacacg gcgcctggcc ttcgacgagc
     3601 tcttctacct gcaagtcggc ctgatccagc gcaagcgtgc ggccgaggat ttgcgcggca
     3661 actcgctcgc cgacggcgcg gaagccatcc cggcgtttct cgacacgctg cccttcaccc
     3721 tgaccggggc gcaacgcagg gccatcgccg acgtcagcgc cgatctgggc ggctcgcggg
     3781 tgatgcagcg gctcgttcag ggcgatgtcg gcagcggcaa gaccgtcgtg gccgcgaccg
     3841 ccgccctgca ggcaacccgc gccgggtacc agtcagcagt catggccccg accgaaatac
     3901 tcgccgggca acttcggacc accctgggtc ggctctacga ggggctcggc gatgccgccc
     3961 cggcgatcga gctgctgacc ggcagcacga agaagcgcga ccggacgaag ctgctggagc
     4021 ggctcgtcaa cagcgaggtc gacgtcctgg tgggcac
//
LOCUS       DASSGX010000472         3562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_736588, whole genome shotgun sequence.
ACCESSION   DASSGX010000472 DASSGX010000000
VERSION     DASSGX010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736588"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(293..1345)
                     /locus_tag="VFI12_08455"
     CDS             complement(293..1345)
                     /locus_tag="VFI12_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056481.1"
                     /translation="MSTTVNQARSAAENWVNEVAAAWPGFDGAFLHGSIAWLAGDAIL
                     PATSDVDIIVVVSGEPPPNPGKFRHEGTLLDVSTLPADQIQSAEDILGISHLAGSLRR
                     SSILADPTGHLVAIQQQIAQNFARPDFTLRRCKHARFKIMMYLGSINSEQPFNENVTS
                     WLFGTGVMTHVLLVAGLRNPTVRSRYVAVHDLLAEYGRLDIHEELLDLLGSANLRRPQ
                     VERHLATMTEAFDAAKSVIRTPVFFATDLSDVARPIAIDGTQEMIERGFYREAMFWIA
                     ATWSRCQHVFSVDAPEMQERFEPAYRAMLADLGITSFEDLLARSEGVQAYLPRLMSIA
                     DEIIAANREISIRTNS"
     gene            1494..>3562
                     /gene="serA"
                     /locus_tag="VFI12_08460"
     CDS             1494..>3562
                     /gene="serA"
                     /locus_tag="VFI12_08460"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HET7056482.1"
                     /translation="MSTTPPSSPFTISVAIDLDGVLTEHPRPLAAAANMKFGMELPES
                     AFIDSAGLNVPMAVRDWVYGEYGPASQLFPAPEAQEFIARIIELLGVENVRILTARPE
                     SSVEMTRAWLRRHGFPEINILFSDDKAAVAAAVGATFAVEDSVRHAVNYAKSGITSFL
                     ILTESTPAYDAHERIWPVQSLMSIIYRLDELKRENERLAALAEAGLPRIVVADQIDAA
                     ARARLAANAEIVDVDGTNLPALLAVLPEADALVVRSETQVTEEVLAAAPKLRVVARAG
                     VGVDNVDLEAATRRGVLVLNAPGANRISAGEHAIALLLAVTRQITIADSATRACNWPR
                     KRIKPIDLAGKTVGIVGLGRVGSVVATRLRAFEMTVVAYDPYIPPERFEELGVERVEY
                     IDLLKRVDIVTYHCPSTDETRHMLDAERIVHLKPGAIVINAARGDVVDQDALAEAVAS
                     GHLRGAGVDVYPYEPCTESPLFSLPNVVLTPHTGGSSAEALANVGEMISTTTLAALTG
                     QAVPNAVNLPPASLLAPDLQRLTSVAAAAGHLLSVVQPELPSAFRVTVRGIVPADVIE
                     HVTSNALAEAINRWTGRWTTPVNARLVAEEIGVTVASLTGANDADVEPSFSFEVLGET
                     SHHVRVMWDRKTAGIVEVDRFALDRALAGELLITHHRDVPGIVGRVGTIMGLYGVNIA
                     GMQVGRH"
BASE COUNT          584 a         1148 c         1218 g          612 t
ORIGIN      
        1 atgacacgcc cggcctcggc gtcgacatcg acgaggagtt ggccgcgaag tacccgttcc
       61 ccgggcacga gctgaacggc gccttcccgg cagtccgccg catcgacggc tccgtcatcc
      121 gctggtaagc gaaaaccttg ccagtggagc ggtcctgagg cggaacgtag agcgcgggcg
      181 taagcctcgc cacggtcatc cccgatcccg gtgaatgttg cctcccgact cgtgggcggg
      241 cggtccttgg tgaccgagcc ttggggagat gtggcagggt tgataccctg cgctatgagt
      301 tggtcctgat tgagatttcg cgattggcgg cgatgatctc gtccgcgatg gacatcaggc
      361 ggggcagata agcctgtacc ccttcgctgc gcgcgagcag gtcctcgaat gacgtgattc
      421 cgaggtcggc gagcatcgcc cggtaggccg gctcgaaccg ctcctgcatt tcaggcgcat
      481 cgacagagaa gacgtgctgg caacgcgacc aggtcgcggc gatccagaac atggcctcgc
      541 ggtaaaagcc gcgctcgatc atttcctggg tgccgtcgat ggcaatcggg cgggccacat
      601 cactgaggtc cgttgcgaag aagacgggcg tcctgatgac ggacttcgct gcgtcgaatg
      661 cttccgtcat ggtcgcgaga tgacgctcga cttgtggtcg cctgaggttc gcagaaccga
      721 ggagatcgag caactcctcg tggatgtcca ggcggccgta ctcggcgagc aggtcgtgga
      781 cggcgacgta gcggctgcgg acggtcgggt tgcggagtcc ggcgacgagc aggacgtggg
      841 tcattacgcc cgtaccgaac agccagctcg tcacgttctc gttgaatggc tgttcagagt
      901 tgatgctgcc caggtacatc atgatcttga accgggcgtg tttgcagcgg cggagtgtga
      961 agtcgggacg tgcgaagttc tgtgcgatct gctgctggat tgcgaccagg tgaccggtcg
     1021 gatcggcaag gatgctcgat ctgcgcaggc ttccggcgag gtgcgagatg ccaaggatgt
     1081 cctcggcgga ctggatctga tcggccggga gggttgagac gtcgagcagg gtgccctcgt
     1141 gccgaaactt gccgggattc ggagggggtt cgccagatac gacgacgatg atatcaacat
     1201 ccgacgtcgc cggcagaatc gcatcaccgg cgagccaggc gatcgagccg tgcaggaacg
     1261 cgccatcaaa ccccggccac gccgccgcga cttcgttcac ccagttttcc gccgcgctcc
     1321 tggcctgatt gacggtggtg ctcatgattt ggtgactccc gcccccattg tgaccgacgg
     1381 gccaagattg tgatcggcac accagggtgc taggctctct ttggcgcagc gcggcgcgat
     1441 tttaggccgc atcgacgccg ccacgcgcga atcacccgga gaatctggat tccatgtcga
     1501 cgactccccc ctcgtcgccg ttcacgatca gcgttgcgat cgacctcgac ggcgtgttga
     1561 ccgaacatcc gcgcccgctc gcggctgccg ccaacatgaa gttcggcatg gagctgcccg
     1621 agtccgcgtt catcgattcg gccggcctga acgtgccgat ggccgtgcgc gactgggtct
     1681 acggcgagta cggaccggca tcgcagcttt tcccggctcc ggaagcgcag gagttcatcg
     1741 cccggatcat cgagctgctg ggcgtcgaaa atgtccgaat cctgaccgcg cggcccgagt
     1801 cgtcggtcga gatgacgcgc gcctggctcc ggcggcacgg ctttcccgag atcaacatcc
     1861 tcttctcgga cgacaaggca gcggtcgcgg ccgccgtggg cgccacgttc gcggtcgagg
     1921 acagcgtccg ccatgcggtc aactacgcga agagcggcat tacctcgttc ctgatcctga
     1981 ccgagagcac gccggcttac gacgcccacg agcggatctg gccggtccag agcctgatgt
     2041 cgatcatcta ccggctcgat gagctcaagc gtgagaacga gcggttggcg gcgctggccg
     2101 aggccggatt gccgcggatc gtggtcgccg atcagatcga tgccgccgcc cgcgctcggc
     2161 tggccgccaa cgccgagatc gtggacgtcg acgggaccaa cctgccggcg ctgttggccg
     2221 tgttgcctga ggccgatgcg ctggtcgtgc gcagtgagac gcaagtgacc gaggaggtgc
     2281 tggccgccgc gccgaagctg cgggtcgtgg cccgggccgg ggttggggtc gacaacgtcg
     2341 atctcgaagc ggctacccgg cgcggtgtcc tggtcctgaa tgcgcccggg gcgaaccgga
     2401 tctcggccgg ggagcacgcg attgctctcc tgctcgccgt cactcgccag atcacgatcg
     2461 ccgacagcgc gacccgcgcg tgcaactggc cccgcaagcg gatcaagccg atcgacctgg
     2521 ccggcaagac ggtcgggatc gtcggactcg gccgcgtcgg ctcggtcgtg gcaacccggc
     2581 tcagggcgtt cgagatgacg gtagtcgcct acgacccgta catcccgccc gaacgcttcg
     2641 aggagctggg cgtcgagcga gtcgagtaca tcgaccttct caagcgcgtc gatatcgtta
     2701 cctaccattg cccctcgacc gacgagacgc ggcacatgct cgacgccgaa cggatcgttc
     2761 acctcaaacc gggcgcgatc gtgatcaacg cggcccgcgg cgatgtggtc gaccaggacg
     2821 cgctggccga ggcggtcgcg tcgggccacc ttcgcggcgc gggggtcgat gtctacccat
     2881 acgagccgtg cacggagagc ccgctcttca gcttgccgaa cgtcgtcctg actccgcaca
     2941 ccggcggctc gagtgctgag gcactggcta atgtcggcga gatgatcagc accaccacgc
     3001 tggccgcgct gaccggccag gcagtgccga acgcggtcaa cttgccgccg gcgtcgctgc
     3061 tcgcgccgga cttgcagcgg ctgacctcgg tcgccgccgc agccgggcac ttgctgtcgg
     3121 tcgtgcagcc ggagctgccg tcggccttcc gggtgacggt gcgcggcatc gtcccggccg
     3181 acgtgatcga gcacgtgacc agcaacgcgc tggccgaggc gatcaaccgc tggaccggcc
     3241 gctggacgac gccggtcaac gcccggctgg tcgccgagga gatcggcgtg actgtcgcat
     3301 cgctgaccgg cgcgaacgac gccgatgtcg aaccgtcctt ctcgttcgag gtcctcggcg
     3361 agacgtcgca ccatgttcgc gtcatgtggg accgcaagac ggcgggcatc gtcgaggtcg
     3421 accggttcgc gctcgaccgg gcgctggccg gcgagctcct gatcacgcac caccgggacg
     3481 tgccgggcat tgtcggccgc gtcggcacga tcatggggct gtatggggtc aacattgccg
     3541 gcatgcaggt tggccggcac ca
//
LOCUS       DASSGX010000473         3054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1062310, whole genome shotgun sequence.
ACCESSION   DASSGX010000473 DASSGX010000000
VERSION     DASSGX010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062310"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            322..981
                     /locus_tag="VFI12_08465"
     CDS             322..981
                     /locus_tag="VFI12_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056483.1"
                     /translation="MSAEFLIGGGRQRDPDLGAGPYATLLGRLIAAAPEVASLPTLLP
                     SPPWTAWDHPAATTWPERDDRGTNLNLVDGPSWIDDNARRVRDLLSAYEAPLRLGHGD
                     WESQNIRWIGDDPLAVHDWDSVIAQPEAAIVGLAAAVWAAQGSPGGAATVAQTKQFID
                     AYQTAFGDWSGQDRAAAWAAGLWVRLFNAKKDASEGGGPRLDRLRNELDERLCRAGLA
                     D"
     gene            complement(1075..1749)
                     /locus_tag="VFI12_08470"
     CDS             complement(1075..1749)
                     /locus_tag="VFI12_08470"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HET7056484.1"
                     /translation="MKLLLTSGGVTNASIREALVDLLGKPVEESNALCIPTAQWGHPM
                     CGPSSVRGFVCGTPPWGGMTSLEWKSLGVLELTALSSIGEERWLPWVREADAFLADGG
                     DTTYLCHWMRQSGLADLLPSLSEMVWVGVSAGSMVMTPRIGADFVRWPSATDDRTLGI
                     VDFSIFPHLDNPDLPSNTMATAEAWAAGLSCPAYAIDDQTAIKATDGVIEVVSEGHWK
                     LLAPND"
     gene            1991..2413
                     /locus_tag="VFI12_08475"
     CDS             1991..2413
                     /locus_tag="VFI12_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056485.1"
                     /translation="MFRRFPIAAVFFAGLVVASTAVLPAAATTNEAVSTHKLIVAVKK
                     TLGPAADKKLAVLVLISQNGGAPAGALATPSKPLTVLQSNSGIYRVRAEIDTSCKGGC
                     DASYRISGSANHKLEVVPRCQLKRSGFVCSNVKIVKIY"
     gene            2462..>3054
                     /locus_tag="VFI12_08480"
     CDS             2462..>3054
                     /locus_tag="VFI12_08480"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7056486.1"
                     /translation="MRNSQQLARMKFVYGDDDVQLRAMLGESLAPRAPDFLFDLALPL
                     LTPESRLLDVGCRDARHLIPLVMRSGCTGIGIDPVERNIDRARAAVTAAGLDQRIELR
                     HGVMEQIEEPTSTVDVVWCRDVLEVIPDLQAGLSEVTRVLKPGGAAVIYTVFATPRLE
                     PGEAAVFHAIGNVPRNLERAWVEEAFRRAGLGVERFE"
BASE COUNT          549 a         1009 c          990 g          506 t
ORIGIN      
        1 ccggtcgtca ggcgcaagga cgctcgcgca gccgagcgct gtgcgtcggc ccggcatgat
       61 cggacggtgc tagcagacga gagcttgggg cggtggtgcg agtcgtcgct tggaagcccg
      121 atcgtggcga cgttgttcga gcatgggaat ctttctcgcg ttctaggcgt gagactcgcg
      181 aaccggcaag aggtcgtgat caagattcgg ccttggcagg accgcttgcg ggcctgcgtt
      241 gcagttcagc ggcacctcgc aatggctgga tatccctgtc cagccccgct tggcaacgtg
      301 gatcacgttg acaggtgggc tgtgagcgcc gaattcctca tcggcggagg gaggcaacgc
      361 gacccggatc tcggcgccgg cccgtacgcg accctgctcg gccggttgat cgccgcggcc
      421 cccgaggtcg ccagcctgcc cacgctgctg ccgtcgccgc cgtggacagc gtgggaccac
      481 cccgccgcca cgacgtggcc tgagcgcgac gatcgcggaa caaacctgaa tctggtggac
      541 ggcccctcgt ggatcgacga caacgcacgg cgtgtgcgag atctgctgag cgcgtacgag
      601 gcgccattgc ggctaggcca cggagactgg gagtcgcaga acattcgctg gatcggggat
      661 gacccccttg ccgtgcacga ctgggacagt gtcatcgctc aaccggaagc agccatcgtc
      721 gggctcgcgg ccgcggtatg ggctgcgcaa ggctctcccg gtggggccgc cacagtcgca
      781 cagacgaagc aattcatcga cgcctaccag accgcattcg gtgactggtc ggggcaagac
      841 cgtgccgccg cctgggctgc agggctatgg gtgcggttgt tcaacgcaaa gaaagacgcc
      901 agcgaaggcg gaggcccgcg gctcgatcgg ctccgaaacg aactcgacga acgcctttgc
      961 agggcaggcc tcgccgactg agaagacgtg ccacggcact gccttcgtca gggacgttcc
     1021 ggaacaccgg cgcagccgag cgcgaatctc gatctcggcc cgctctcgca cacctcagtc
     1081 gtttggtgcg agcagtttcc agtgcccctc agagacgacc tcgatgacgc cgtcggtcgc
     1141 cttgatggcg gtctggtcgt cgatcgcgta cgcgggacat gacaagccgg ccgcccatgc
     1201 ttcggcggtg gccatcgtgt tcgacggcag gtcagggttg tcgaggtgcg ggaagatcga
     1261 gaagtcgacg atccccaggg ttcggtcgtc cgtcgccgac ggccatctca cgaagtcggc
     1321 accgatccgg ggggtcatca ccatgctccc ggcgctcacc ccaacccaga ccatctcgga
     1381 gagagagggc aggaggtccg ccagcccgct ctgccgcatc cagtggcaca ggtaggtcgt
     1441 gtcgcccccg tccgccagga aggcatcggc ctcccgcacc cagggaagcc agcgctcttc
     1501 gccgatgctg gatagtgccg tcagctcgag cacgcccagc gacttccact ccagcgaggt
     1561 catcccaccc cacggaggcg tgccgcagac gaagccccgc actgacgatg ggccgcacat
     1621 cgggtgcccc cattgcgcag tgggaatgca aagggcattg gactcctcga ccggcttccc
     1681 gagcaggtcg accagcgctt cccggatgct cgcgttcgtg acgccgccgg acgtaagaag
     1741 aagcttcaag tgacccccaa aatcgttggc agtggcgagg atgatcgtgc tggcggatcc
     1801 gccctgctag agaccggcac gcctgccaga acttatcggt tgcgaccagg agccaactca
     1861 ccggcggcgt cagcggatgg aaggcgaagg gcgggcgtcg aaccgccgtt ttccccgacc
     1921 gcctggaaca tcgatccccg gcggggaaac gcggttcgca aggactcgac aagcgcaata
     1981 cccttggtgc atgttccgtc gctttcccat tgcagcagtc ttcttcgccg gtctcgtcgt
     2041 tgcctcgacg gccgtcttgc cggcggcagc cacgacgaat gaggctgtaa gcacccacaa
     2101 gctgatcgtg gctgtcaaga agaccctggg cccggcggcc gacaagaagc ttgccgtcct
     2161 cgttctcatc agccagaacg gcggcgctcc tgcgggagcc ctggcgacgc cgagcaagcc
     2221 gcttactgtt cttcaaagca atagcggcat ctaccgtgta agagccgaga tcgacacctc
     2281 ctgcaaaggc ggctgtgatg cgagctatcg aatttcgggg tcggccaatc acaagctcga
     2341 ggtcgtcccc aggtgtcagc tcaagcgctc aggcttcgtc tgcagcaacg tgaagatcgt
     2401 caaaatctac taatgagcag ctctgctccc ctgggccagc cctccgccac gaatgcctga
     2461 gatgaggaac tcgcagcagc tcgcccggat gaagttcgtc tatggggacg acgacgtgca
     2521 gctccgcgcg atgctcggcg aaagcctcgc gccgcgggca cccgacttcc tcttcgacct
     2581 cgctcttccg ctactgacgc ccgagagcag actgctcgac gtcggctgtc gcgacgcacg
     2641 acacctgatc cccctcgtca tgcggtctgg atgcaccggc atcggcatcg acccggtcga
     2701 gcgcaacatc gaccgcgcgc gggctgccgt caccgctgca ggtctcgacc agcgaatcga
     2761 gctccgccac ggcgtcatgg aacagatcga ggagcccaca agcaccgtcg atgtggtctg
     2821 gtgccgcgac gtgctcgagg tcattcctga cctgcaagca ggcctgtccg aagtcacacg
     2881 cgtgctcaag ccgggcggcg ctgcggtcat ctacaccgtc ttcgcgacgc cgcggctgga
     2941 acccggcgaa gctgctgtgt ttcacgcgat cggcaacgtg ccgcggaatc tcgaacgtgc
     3001 gtgggtcgaa gaagcattca ggcgcgcggg tctcggtgtc gagcggttcg agga
//
LOCUS       DASSGX010000474         2157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1062749, whole genome shotgun sequence.
ACCESSION   DASSGX010000474 DASSGX010000000
VERSION     DASSGX010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1062749"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VFI12_08485"
     CDS             complement(<1..428)
                     /locus_tag="VFI12_08485"
                     /inference="COORDINATES: protein motif:HMM:NF019236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HET7056487.1"
                     /translation="MKLSRRVLLHAGSGILLARNVLSLDPAVAQDATPTPAPKRIIAV
                     DANTGDDNNDGSQGNPFKTISRACQAIQPGDLVQIAGGKYSGEVNVTTSGNADAPISI
                     AAVDGATVTLSTGGFKIAADYITVQGITVEKVTDSAEAGI"
     gene            complement(425..1888)
                     /locus_tag="VFI12_08490"
     CDS             complement(425..1888)
                     /locus_tag="VFI12_08490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7056488.1"
                     /translation="MAANRIPPVPHNPRRRASRGKPLALGFMCAALVGAIGVGLYRGL
                     GASLWTTLNREIVGTPLYLPLGAFGLMRWTLWLCKKIPAAFYRPITAPYWTSSTIVTP
                     VYDEDPVIFRRAIESWQVNWPDAIIAVIDETDTACIAIAREYPDISVIVTATPGKRPA
                     LADGILAATTDIVVLVDSDTIFEPRVLERILKPFADPRIGGVGTRQKVYIRGTVWQRI
                     ADMFLDIRYEDEAPALTRMGQALSCLSGRTAAYRRAVVLPLLDDFLGETFFGKPCMSG
                     EDKRLTNLVLKAGYNTYYQGDALVWSTFPPDFTTFIKQRIRWTRNSYRCDLRAMWEGW
                     VWKHWYLSFQLFDKSISPYTLLLGLTFFLWSLLNGHWSVSLLIIGWWLTSRTVKLWPH
                     LRRYPQDFALLPVFIGVTFLMTFVKAYALSTVHHHKWLTRRVEVVNGETVRSNSAQAS
                     RPDRTPFLTRAAGSMIMMTILGAVQLAAWALLRTVTR"
     gene            complement(1918..>2157)
                     /locus_tag="VFI12_08495"
     CDS             complement(1918..>2157)
                     /locus_tag="VFI12_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056489.1"
                     /translation="GTMRVNDILEGHVMAPTSMHEVIPASELINDARSSVDHAVNVAI
                     SGALASEFVDLPPEPVVAMPMPIAHHAAGEEIVAP"
BASE COUNT          377 a          673 c          750 g          357 t
ORIGIN      
        1 aagatgcccg cctcggcgga atcggtcacc ttctcgacgg tgatgccctg gacagtgatg
       61 tagtcggcgg cgatcttgaa cccgcccgtg ctgagggtca cggtcgcgcc gtctaccgcg
      121 gcaatcgaga tcggggcatc cgcgtttccg gaggtagtga cgttgacctc gcccgagtat
      181 ttgcctccgg cgatctgtac cagatcgccc ggctggatcg cctggcaggc ccggctgatc
      241 gtcttgaagg ggttgccctg gctgccgtcg ttgttgtcat cgcccgtgtt tgcgtcgacg
      301 gcgatgattc gcttcggggc cggcgtcggg gtcgcatcct gggcgactgc cgggtcgaga
      361 gagaggacat tgcgggcgag cagaatcccg gagccggcgt gcagcagcac acggcggctc
      421 agcttcatcg ggtcaccgtc cgcagcagcg cccatgccgc cagttgtacg gcgccgagga
      481 tcgtcatcat gatcatcgag cccgcggcgc gggtcaggaa gggagtgcga tccggccggc
      541 tggcctgggc cgaattgctc cgcacggtct cgccgttgac gacctcaacc cgtcgcgtca
      601 gccacttgtg atggtggacg gtggagagcg cgtacgcctt gacgaatgtc atcaggaagg
      661 tgacgccgat gaagaccggt agcagcgcga aatcctgcgg gtagcgacgg aggtgcggcc
      721 acagcttgac cgtccggctc gtcaaccacc agccgatgat cagcagcgag accgaccagt
      781 ggccgttgag gagcgaccag aggaagaagg tcaacccgag caacagcgta tacggcgaga
      841 tcgacttgtc gaagagctgg aaggagagat accagtgctt ccagacccac ccttcccaca
      901 tcgcgcggag atcgcaccgg taggagttgc gcgtccagcg gatgcgctgc ttgatgaagg
      961 tcgtgaagtc gggtgggaaa gtcgaccaaa ccagcgcatc gccctggtag taggtgttgt
     1021 atcccgcctt gaggacgagg ttggtcagcc gcttgtcctc gccactcatg catggcttgc
     1081 cgaagaaggt ttcgccgagg aaatcgtcca ggagcggcag caccaccgcg cgccggtagg
     1141 ccgcggttcg ccccgagagg caggagagcg cttgccccat ccgggtcagg gccggagcct
     1201 cgtcctcgta ccgaatgtcc aggaacatgt ccgcgatccg ctgccagacc gtgccccgga
     1261 tatacacctt ctgccgcgta ccgacgccgc cgatcctggg gtcggcgaac ggtttcagga
     1321 tccgctcgag cacgcgcggc tcgaaaatgg tgtccgagtc gaccagcacg acgatgtcgg
     1381 tcgtggcggc gaggatgccg tcagccaggg cggggcgctt gcccggcgtg gccgtgacga
     1441 tcaccgagat gtccggatac tcccgggcga tggcgatgca ggctgtatct gtctcgtcga
     1501 tgaccgcaat gatcgcgtcg ggccagttca cctgccacga ctcgatcgcc cggcgaaaga
     1561 tgaccggatc ctcatcgtag accggggtga cgatggtcga gctcgtccag tacggcgcgg
     1621 tgatcggccg gtaaaaggcg gccgggatct tcttgcagag ccagagcgtc cagcgcatca
     1681 gcccgaaggc gccgagcggc aggtagagcg gcgtccccac gatctcgcgg ttgagcgtgg
     1741 tccagagcga tgctcccagc ccgcgataca gcccgacccc gatcgcgccc acgagcgcgg
     1801 cgcacatgaa gccgagcgcg aggggtttcc ccctcgacgc ccggcggcgg ggattgtgcg
     1861 gaaccggcgg gatccggttg gcggccatgg gtgcgggctt cccgggcagc gcaagcgcta
     1921 cggcgccacg atctcctcgc ccgccgcgtg atgcgcgatc ggcatcggca tggcgacgac
     1981 gggctcaggc gggagatcga cgaattcgga cgcgagcgct ccgctgatcg cgacattgac
     2041 ggcgtgatcg accgaggagc gggcatcgtt gatcagctcc gaggcaggaa tcacctcgtg
     2101 catggaggtc ggcgccatga cgtgcccctc caggatgtcg ttgacgcgca tggtgcc
//
LOCUS       DASSGX010000475         2809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_847642, whole genome shotgun sequence.
ACCESSION   DASSGX010000475 DASSGX010000000
VERSION     DASSGX010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_847642"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VFI12_08500"
     CDS             <1..219
                     /locus_tag="VFI12_08500"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET7056490.1"
                     /translation="ATRYASHTAGAVTHGGVLGSMAAQLIGTPPNDWARYQIKNCSVT
                     HLEIGQEGSIFHRFNDCAHLDLVEEPMR"
     gene            216..992
                     /locus_tag="VFI12_08505"
     CDS             216..992
                     /locus_tag="VFI12_08505"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7056491.1"
                     /translation="MRVVALGGSAAGPNTGAGCAGFLVTEGGTSVVIDLGPGTLLELR
                     KHVDYRSLSAIVVSHYHLDHILDLGALRFLAKYNPVPMDRKIPLYVPPGSCNRFGQWG
                     SVFGDQAENEFLEAVFDIGEYDPSQSYAFGDISICFARTVHPVPAWALRVAGLGGGDF
                     GYTADTGPGAMLDLADFFRDVTLLTSEATEPPDPRPWNGARGHLTPAEAGRLATDAGA
                     KTLILTHRWEELGLELGAAEAQTTFAGPVMIARPGLTVFI"
     gene            989..1867
                     /locus_tag="VFI12_08510"
     CDS             989..1867
                     /locus_tag="VFI12_08510"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HET7056492.1"
                     /translation="MTAGPDATFTVGHWTDQANRTGCTVIYFDRLVPAVVDVRGGAPG
                     TRETDLLASDRLVGRVDAILLAGGSANGLAAADGVMQHLRERGRGFETAAGPVPIVPA
                     AVIFDLAVGSATWPTADGGYEACEAARPLEESRMGRIGAGTGATYRKLWPNRDPAPGG
                     FGWSAARISDSVAIHALAVVNAVGDVVEGDWVDGRPALLGEMQEQMDRSATTLVTVIV
                     DGAADQRTLRRVAAAAHDAMARTIVPCHTLWDGDLVFAAQTGDVVEGALPDALRLTVA
                     AELAVEQAVKSAVRPS"
     gene            complement(1956..2357)
                     /gene="rpsI"
                     /locus_tag="VFI12_08515"
     CDS             complement(1956..2357)
                     /gene="rpsI"
                     /locus_tag="VFI12_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HET7056493.1"
                     /translation="MTEKTRYHYGTGRRKSAVARVRMYPGKGAITVNGKTVPDYFGGR
                     TNLHAVLESPLRLTNTAGRYDIIVKVVGGGVAGQIGAVRHGIARALARSDEELRPILK
                     RAKLLTRDARVKERKKVGLKRARKAPQYTKR"
     gene            complement(2375..2809)
                     /gene="rplM"
                     /locus_tag="VFI12_08520"
     CDS             complement(2375..2809)
                     /gene="rplM"
                     /locus_tag="VFI12_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HET7056494.1"
                     /translation="MDRRTFSPKAGDIQRDWFIVDADGMTLGRLATVIASTLRGKNKP
                     TYAPHMDMGDFVIVINAEKIKVTGNKETEKFYYRHSGYPGGLRADMLRDVRRRFPDRI
                     ITSAVRGMLPRTTLGEKQLSKLKVYAGANHPHAAQQPKPLPL"
BASE COUNT          485 a          907 c          917 g          500 t
ORIGIN      
        1 gcaacgcgct atgcgtcaca caccgcgggc gcggtgacgc acggcggcgt ccttggctcg
       61 atggccgccc aattgatcgg aacgccgccg aacgactggg cgcgctacca gatcaagaac
      121 tgcagcgtga ctcacctcga aatcgggcag gaggggtcga tcttccaccg cttcaacgac
      181 tgcgcgcatc tcgacctggt cgaggagccg atgcggtgag ggtcgtcgcc ctcggtggga
      241 gcgcggccgg tccgaacacg ggcgccggct gcgcaggctt ccttgtcacc gagggcggca
      301 catcggtagt catcgacctg gggccaggga cgttgctcga gctccggaaa cacgtcgatt
      361 accggtcgct cagcgccatc gtggtctccc actaccacct ggatcacatc ctggatctcg
      421 gagcgctacg tttcctcgcc aagtacaatc cggtgccgat ggatcgaaag atcccgctct
      481 acgtccctcc cggaagctgc aacaggttcg ggcagtgggg gtcggttttc ggggaccagg
      541 cagagaatga attcctcgag gcagtcttcg acatcggcga atatgacccg agccagtcgt
      601 acgctttcgg ggacatctcg atctgcttcg cgcgaacggt gcatccggtt cccgcctggg
      661 cgttgcgcgt ggcaggattg ggaggcggcg atttcggcta taccgccgat accgggcctg
      721 gagcaatgct ggatctggcg gacttcttcc gcgacgtcac gctcctgacg agcgaggcaa
      781 ccgaaccccc ggacccgcgg ccatggaacg gagcgcgcgg gcacctcacc cccgcggaag
      841 ccggccgact ggcgaccgac gccggtgcga agacgctcat tcttacgcac cgctgggaag
      901 agctggggct ggaactcggc gcggcggagg ctcagacaac cttcgccggt ccggtgatga
      961 tcgcccggcc cggactgaca gttttcatat gaccgctgga cctgacgcca cattcacggt
     1021 cggccactgg acggatcagg cgaaccgtac cggctgcacg gtcatctact tcgaccggct
     1081 cgtgccagca gtcgtcgacg tccgcggggg ggcgcccggg acccgggaaa ccgatttgct
     1141 cgcgtccgat cgcctcgtcg gccgggttga tgccatcctg ctcgccggtg gcagcgcgaa
     1201 tggcctggca gcggcggacg gcgtcatgca gcacctccgg gagcgcggcc gcggattcga
     1261 aaccgccgca ggaccggtcc cgatcgtccc agcggccgtg atcttcgacc tggctgtcgg
     1321 gagcgcgacg tggccgaccg cggatggcgg atacgaggcg tgcgaggcgg cgcgaccgtt
     1381 ggaagagtcc cgcatgggcc gcatcggcgc ggggaccgga gcgacttatc gcaagctctg
     1441 gccaaaccgc gatccggcgc ccggcggctt cggatggagt gctgcgcgca tctctgatag
     1501 cgtcgcgatc cacgcactcg ccgtcgtcaa cgcggttggg gatgtcgtcg agggtgattg
     1561 ggtcgatggg cggccagcgc ttctcggaga gatgcaagag cagatggatc gatccgccac
     1621 cacgctcgtc accgtcatcg tggacggcgc tgccgaccag cgaacgctgc gcagagtcgc
     1681 cgccgcggcc cacgacgcca tggcccgcac gatcgtgccc tgtcacacgc tgtgggacgg
     1741 cgatctggtt tttgccgctc aaaccggcga cgtcgtcgaa ggtgcgctac cagatgctct
     1801 gaggctgacc gtcgcagccg agcttgccgt cgagcaggca gtgaaatcgg cggtcaggcc
     1861 gtcgtaacga ctttcccatg cgcaacgaac acacaacagg gccgatgcaa atgcatcggc
     1921 cctgtttgat cattcgaatt cggcgagtcg ggtgtttacc gcttcgtgta ctgaggcgcc
     1981 ttgcgggccc gcttgagtcc gaccttcttg cgctccttga cgcgcgcatc gcgagtcaat
     2041 agcttggcgc gcttcaggat tggccggagt tcctcgtcgg agcgggccag cgcccgggca
     2101 ataccgtgac gcactgcacc gatctgcccg gcgactccgc cgcccacgac cttaacgatg
     2161 atgtcgtagc gaccggccgt gttggtcagg cggagcggcg actccaggac agcatgcaaa
     2221 ttggtgcgcc caccgaagta atcagggacg gtcttgccgt tcaccgtgat cgcgcccttg
     2281 cccgggtaca tccgaacgcg ggcgaccgca gacttccgac gacctgtgcc gtagtgatat
     2341 cgagtcttct cagtcattga ttcctcagtc tcccctacag cggcagcggc ttcggctgct
     2401 gcgccgcgtg cggatgattc gcgccagcat agaccttgag cttggacaat tgcttctcac
     2461 cgagcgtggt ccgcgggagc atgccacgca ccgccgacgt gatgatgcga tcggggaaac
     2521 gccgccgcac gtcgcgcagc atatccgccc gaaggccacc cgggtatccg gaatgccggt
     2581 agtagaactt ctcggtctcc ttgttgcccg tgaccttgat cttctcggcg ttaatgacga
     2641 tgacaaaatc gcccatgtcc atgtggggcg cgtaggtcgg cttattcttg ccgcgaagcg
     2701 tggaggcgat cacggtcgcg agccgaccca gggtcatgcc atccgcgtca acgatgaacc
     2761 aatctcgttg aatgtcgcca gctttaggag aaaaggttcg tcgatccat
//
LOCUS       DASSGX010000476         3972 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_519320, whole genome shotgun sequence.
ACCESSION   DASSGX010000476 DASSGX010000000
VERSION     DASSGX010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3972
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519320"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VFI12_08525"
     CDS             <1..301
                     /locus_tag="VFI12_08525"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HET7056495.1"
                     /translation="SGQQGQSQAQTNPTVEMVDIGYNPNALTIPANTDVTVTLKNTGQ
                     LPHTFDIDALNIHSDQVQPGQTATVKINAAAGSYQFYCREPGHKEAGMVGTLTVQ"
     gene            405..481
                     /locus_tag="VFI12_08530"
     tRNA            405..481
                     /locus_tag="VFI12_08530"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:439..441,aa:Glu,seq:ctc)
     gene            662..1834
                     /locus_tag="VFI12_08535"
     CDS             662..1834
                     /locus_tag="VFI12_08535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7056496.1"
                     /translation="MVFAAKPEHELLRAEMRTLCAQFGVEYWRDLDLERAYPERFVRT
                     LAEAGYLSALIPREYGGLGLGVSEASIILEEINRSGGSSAACHAQLYILSALVRHGSD
                     QQKQSWLPQIASGERRLQAFSVTEPEAGSDTTAIRTTAVRDGDQYVVNGHKNWTSRIE
                     QSDLLLLLARTTPREEVADRTRGLSLFLVDLREVRQRQPDAIEARPVRVMFNYETYQV
                     TYRNLRVPADALIGEEGLGFRSVIDGWNAERILLAAECIGDGYWFVEKASRYASERDV
                     FGRPIGQNQGVQFPIAHAYAQLRAADLLRYQAASLYDRGDQCGAEANMAKLLASEASW
                     AAANACLDTHGGNGFVAEHDVERKFRETRLFQVAPINNNLVLAYLAHHVLGMPRSY"
     gene            2055..3077
                     /locus_tag="VFI12_08540"
     CDS             2055..3077
                     /locus_tag="VFI12_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7056497.1"
                     /translation="MYRRLGLMALLVLVTFATAAQSRSVHAHETDSTASLCTGQPQGA
                     ACSLVTKAVVAELPAPPAILALVRMTINPGVQFTDLSVPGPTVLLVETGTLTVDMSGE
                     TDEDEQLEQLEGFVERAPANGTPTPSSGPVQIASVGIFRITLQAGDRLLIPSDWPHAM
                     RNDTGTPISILAAAVTPPEPATGGPAWPPAGAWPPGLPDGVKVRSLDAGYRAVTDLPR
                     TDATEILVERVTLQSGTSLALGPGAPHLLTVENGVLSVQCAAACPARHKGSTSTVPAS
                     PSPVATGADGGTVTLASGESVLIPDAVQATISTGAGSETGPVSLISLTVAPSNEDPGR
                     QGEHAD"
     gene            complement(3113..>3972)
                     /locus_tag="VFI12_08545"
     CDS             complement(3113..>3972)
                     /locus_tag="VFI12_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056498.1"
                     /translation="YFGNAATPLQPGCPSSCHGYYSYDVGTWHIVVLNSNCSQAGGCG
                     VGSAQEKWLRADLAAHTNTCSVAYMHHPRFSSGQIGSFPTMQPLWQAFYDNGGDLVLA
                     GHDHNYERFAPQTPGGQADSNWGIREFVVGTGGRNTSALGTIKANSEVRNTNTFGILK
                     LVLRPTGYDWQFIPISGSTFTDSGSGNCHGVPGTADVGGAVATGAGPGNLAGLPVAMA
                     VASDPAGQPHRGQGTDPMTFLLTALPAAAFVGSWRSSLIRRRGARHVIPPTRPNHVTR
                     SSGYVGRSE"
BASE COUNT          757 a         1308 c         1239 g          668 t
ORIGIN      
        1 gagcggtcaa cagggtcaga gccaagcgca gaccaacccg acggtcgaga tggtggacat
       61 cggttacaac ccgaatgcgc taacgatccc ggccaacacc gatgtcacgg tgacgttgaa
      121 gaacactggt caattgccgc acacctttga catcgacgcg ctcaacatcc actcggatca
      181 ggtccagccg ggccagacag cgacggtgaa gatcaacgcg gcggcgggtt cgtaccagtt
      241 ctattgccgc gagcccggtc acaaggaagc gggcatggtc ggcaccctga cggtgcaata
      301 acaccccgaa agcgccgatg cgccgcgggt cgcggcgcat cggtttcacg cctgatttcg
      361 gtttgaccaa cgtgcactgt gtacgtataa ttacagactg atttggcgca ttcgtctagc
      421 ggcctaggac accgccctct caaggcggag atcaggggtt cgaatcccct atgcgctacc
      481 aaccgcccgt gcaatgccgg gcagtccaga aaacccgcgc aaatgcgcgg gtttttgcgt
      541 ttgccggtcg aaaatgtccg gagagaccca ctccatcccg cccgttctcg agatctgccc
      601 tctctgcctc gctgctacac tccgccagcg gaatcttgac gcgcggacgc ggagtatagc
      661 ggtggttttc gcagccaagc cagagcacga gctgctgcgc gccgagatgc gcacgctctg
      721 cgcacaattc ggcgtcgagt actggcgtga tctcgacctg gaacgggcat accccgaacg
      781 gttcgtgcgg acgctcgccg aggccgggta tctctcggcg ttaattccga gggagtacgg
      841 cggactcgga ctcggcgtga gcgaggcgag catcatcctc gaggagatca accgctccgg
      901 tggctcatcg gcagcatgcc acgcccagct ctacattctc agcgcgctcg tccggcacgg
      961 aagcgaccag cagaagcaga gctggctgcc gcagatcgcg tcgggcgagc ggcggctcca
     1021 ggccttttcg gtcaccgagc ccgaggccgg gtccgacacc accgcgatcc gcaccacggc
     1081 ggtccgcgac ggcgaccagt acgtcgtcaa tggccacaag aactggacaa gccggatcga
     1141 gcagtcggac ctgctcctgc tcctggcccg gaccacgccg cgagaggagg tggccgatcg
     1201 cacccgcggt ctcagcctct tcctggtcga cctgcgagag gtacggcaac ggcagccgga
     1261 cgcaatcgag gctcgtccgg tccgggtcat gttcaactac gagacgtacc aggtgaccta
     1321 ccggaatctc cgcgtcccgg ccgatgcgct gatcggcgag gaaggtctgg gatttcgctc
     1381 tgttatcgac ggctggaacg ccgagcgaat cctgctcgcg gcggagtgca ttggcgatgg
     1441 ttactggttt gtcgaaaagg cgtcccgata cgcgagcgaa cgggatgttt tcggccgccc
     1501 aatcggccag aaccaggggg tccagttccc catcgctcat gcatacgccc agctccgtgc
     1561 cgccgacctg ctgcggtacc aggcggcatc gctctatgac cgcggcgacc aatgcggcgc
     1621 tgaagcgaac atggcgaagt tgctggcgag cgaggcgagt tgggcagccg ccaacgcctg
     1681 cctcgatacg cacggtggca acggcttcgt cgccgagcat gatgtggagc ggaagttccg
     1741 ggagacgcga ctgttccagg tcgccccaat caacaacaac ctcgtgctcg cctacctcgc
     1801 ccaccacgtc ctcggcatgc ccaggtcgta ttgacccggg catgccggca tcgaccccgc
     1861 cacgactcca gaattcgctc aactgcgttc cggctcacgg tgccacatgc tcgacgcccg
     1921 gcatatgcag gagtagcatg caggtacgac gatcccgctg tgcgccgtga atactccggg
     1981 acctcatcgg ctcgactgac gtttcgagca gtggggcgac aaggatcaac cggggcaaac
     2041 caggagcgag ttgaatgtac cgtcggctgg gcctgatggc ccttctggtc ctcgtcacgt
     2101 ttgcgactgc cgcccaatca cgatcagttc atgcccacga aacggacagc accgcgtcgc
     2161 tatgcaccgg tcagccacaa ggagccgcgt gctcgctcgt aacgaaggcg gttgtcgccg
     2221 aactaccggc tccgcccgcg atcctggccc ttgtccgcat gaccatcaac ccgggtgttc
     2281 aattcaccga cctctccgtg ccgggcccca ccgtgctcct ggtcgaaacg ggcacactca
     2341 cagtggatat gtccggggaa accgatgagg acgaacaact cgagcagttg gaaggattcg
     2401 tagagcgcgc gccggccaac ggcactccga cgccgtcatc cggcccggta cagattgcgt
     2461 cagtcggcat ctttcggatc acgcttcagg ccggcgaccg tctgctgatt ccctccgatt
     2521 ggccccacgc gatgcggaac gataccggga cgccaatttc catcctggcg gcagccgtga
     2581 ccccccccga gcctgcgacc ggtggcccgg catggccccc ggccggcgcc tggccaccgg
     2641 gactcccgga cggtgtgaag gtgcggtcgc tggacgccgg gtatcgagct gtcactgatt
     2701 tacctcgcac cgatgcgacg gagatcctcg tggaacgggt gaccctgcaa tccggaacgt
     2761 cgctcgccct cggaccggga gccccacacc tcctgacggt cgagaacggt gtcctttctg
     2821 tacagtgcgc cgctgcatgc cctgccaggc acaagggctc gacatcgacc gtgccggcct
     2881 ctccgagccc tgtggcgaca ggagctgatg gtggcacggt caccctggcg tcaggcgaaa
     2941 gcgtccttat cccggatgcc gtccaggcga cgatttccac cggagccgga agcgaaacgg
     3001 ggccggtctc gctcatcagc ctcaccgtgg ccccgagcaa cgaagacccg ggaagacaag
     3061 gcgagcacgc agactgaccg ttcagcacaa agacggggcg ggttgagccg ccttattcag
     3121 accgcccgac gtatccggaa gacctcgtca cgtggttcgg acgagtcggc ggaatcacgt
     3181 gacgtgcacc acgccgccgg atgagtgagc ttcgccagga gccgacgaag gcggcagccg
     3241 gcagcgcagt cagcaagaat gtcatcgggt ctgtgccctg accgcgatgg ggttggccgg
     3301 cgggatccga cgctaccgcc atcgcaaccg gcaaccctgc cagattgccc ggccccgccc
     3361 cggtcgcgac ggcgccgcct acgtctgcgg tccccggaac gccgtggcag ttgccgctcc
     3421 cggagtcggt aaacgtggaa ccggagatcg gaatgaactg ccaatcatag ccggtcggac
     3481 gcaagaccag tttgaggata ccgaaggtgt tcgtgttccg gacctcgctg ttcgctttga
     3541 tcgtcccgag cgccgatgtg ttgcgaccac cagtgccgac aacgaactca cgaatacccc
     3601 aattcgagtc ggcctggccg ccgggtgtct ggggcgcgaa ccgttcgtag ttgtgatcgt
     3661 gacccgccag caccagatcg ccgccgttgt catagaacgc ttgccaaagc ggttgcatcg
     3721 tcgggaagct gccaatttgc ccggaactga agcgagggtg gtgcatatac gccacggaac
     3781 atgtgttggt atgggcagcc aggtccgcgc gcagccactt ctcctgggcc gagccaactc
     3841 cgcagccacc ggcctgcgaa caattggaat tgaggacgac gatgtgccag gtaccgacat
     3901 cgtagctgta gtacccgtgg caactgctcg ggcagcctgg ctgcagtggg gtggctgcat
     3961 tcccgaagta gg
//
LOCUS       DASSGX010000477         2321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_737393, whole genome shotgun sequence.
ACCESSION   DASSGX010000477 DASSGX010000000
VERSION     DASSGX010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_737393"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1200)
                     /locus_tag="VFI12_08550"
     CDS             complement(<1..1200)
                     /locus_tag="VFI12_08550"
                     /inference="COORDINATES: protein motif:HMM:NF023222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HET7056499.1"
                     /translation="MIRTGDEYRESIRDGREVWMSGEKIEDVTTHPAFKPAVDARALI
                     YDMQNRPDCQDVMTYVDEGTGQRCAIALKLPMTRQDWRDKRRAVDAVLDEVGGVVTRV
                     GDETIGEMWSLYDGQDVLNEVNSQFSENITRHIARAIIEDPFHVSANTDPKGDRSKRP
                     QDQDPDMLLHVVRETDRGIVVRGAKYETAAAYANQAFVKPTIANWGDSELSDYALGFI
                     VNMGAKGLKHICRTGFAGKKPAEDYPLSNRVDEVDTLVIFDDVEIPWEDVLFYRHTRA
                     ASYIRATLHRYSAFPFVQRVLRLADLMIGVALFNAKQTGLEKQQAVQEKLAELAIYRE
                     GINAHLTASIALAETSPGGLLMPNQSLLYTGRVLASSQLPAMMHIARELCGGQICITP
                     DSATFAHP"
     gene            complement(1297..1887)
                     /locus_tag="VFI12_08555"
     CDS             complement(1297..1887)
                     /locus_tag="VFI12_08555"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HET7056500.1"
                     /translation="MSEDDPVELRTAFIEAMSRAATGVTIVTTGGPSGRVGQTVSAMC
                     SVSADPPSLLVCIKTTSVIVNAIRQHRAFGVNVLRADQRRVADTFAGRSTWGTPYNFD
                     LAHWDTLVTDVPLLVGAVGRFDCRLSTVLEVGSHSVLFGDVLAADSAAGTPLLYARRS
                     YGEHMEIPRQIDEGMLDLDVRLTWEDLDKGTRREGE"
     gene            complement(2066..>2321)
                     /locus_tag="VFI12_08560"
     CDS             complement(2066..>2321)
                     /locus_tag="VFI12_08560"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HET7056501.1"
                     /translation="GTAFQRKVWTELRRIPRGERRSYQQIATQVGSPSAVRAVANACG
                     ANPTALAVPCHRVVRSDGGLGGYRWGADRKQTILALEASA"
BASE COUNT          354 a          737 c          806 g          424 t
ORIGIN      
        1 ggggtgcgcg aacgtcgccg agtcgggcgt gatgcagatc tgcccgccgc acagctcgcg
       61 ggcgatgtgc atcatcgccg gcaactggct cgaggcgagg acgcgaccgg tgtagagcag
      121 gctctggttc ggcatcaaca agccgcccgg gctggtttcg gccagcgcga tcgaggcggt
      181 caggtgggca ttaatcccct cgcggtagat cgccagctcg gccagcttct cctggaccgc
      241 ctgctgcttc tcgagcccag tctgcttggc gttgaagagg gcgacgccga tcatcaggtc
      301 ggcgaggcgg agcacgcgct ggacgaacgg gaaggcgctg tagcgatgca gcgtggcccg
      361 gatgtagctg gcggcgcggg tgtgccggta gaagaggaca tcctcccacg ggatttcgac
      421 gtcatcgaag atgacgagcg tgtccacctc gtccacccgg ttagagagcg gatagtcctc
      481 ggccggtttc ttgcccgcga agccggtccg gcaaatgtgc ttcagcccct tggcgcccat
      541 gttgacgatg aaaccgagcg cgtagtccga tagctcggaa tcgccccagt tcgcaatggt
      601 gggcttgacg aaggcctggt tggcataggc cgcggccgtc tcgtacttgg cgccccgaac
      661 gacgatgccc cggtccgtct cgcggacgac gtggagcagc atgtccgggt cctgatcctg
      721 cggtcgcttc gaccggtcgc ccttgggatc ggtgttggcc gagacgtgga acgggtcctc
      781 gatgatggcg cgggcgatgt ggcgggtgat gttctcggag aattgcgagt tcacctcgtt
      841 gaggacgtcc tggccgtcgt agagcgacca catctcgccg atcgtctcgt cgccgacccg
      901 ggtgacgacc ccgccgacct cgtcgagcac ggcgtcgacg gcgcggcgct tgtcccgcca
      961 atcctgccgg gtcatcggca gcttgagggc gatcgcgcag cgctggccgg tgccctcgtc
     1021 gacgtaggtc atcacgtcct gacaatcagg tcggttctgc atgtcataga tgagggcgcg
     1081 ggcgtcgacg gccggcttga aggcgggatg cgtggtgaca tcctcgatct tctcgccgct
     1141 catccagacc tcgcggccgt ccctgatcga ctcgcgatac tcgtcaccgg tcctgatcac
     1201 tcgcgtactc cttgtggtcg gtggttcgtg gctaatggtt agtgattggt ttgccgtttt
     1261 tcgttcttcg cccgctcgcc tcgttcaact tcgcgcctac tcgccttcgc gcctcgtgcc
     1321 cttatccagg tcttcccagg tcaatcgcac gtccaggtcg agcatgccct catcgatctg
     1381 gcgcggtatt tccatgtgtt caccgtagct gcgtcgagca taaaggagcg gagtgcccgc
     1441 tgccgagtcc gccgcgagga catcgccgaa caggacggag tgggagccga cctcgaggac
     1501 tgtactgaga cggcagtcga aacggccgac ggcgccgacg agcagcggga catcggtcac
     1561 gagcgtgtcc cagtgggcga ggtcgaagtt gtaaggcgtc ccccaggtcg agcggccggc
     1621 gaaggtgtcg gccacgcggc gttgatcggc acgcaggaca ttgacgccga aggctcggtg
     1681 ctgccggatg gcgttgacga ttacgctggt ggttttgatg cagacgagca gggatggggg
     1741 atcggccgag acggagcaca tcgcgctgac cgtctgcccg acgcggccgg aagggccgcc
     1801 ggtggtaacg atcgtgacgc cggtcgctgc ccggctcatc gcttcaatga acgcggtccg
     1861 cagctccacc gggtcgtcct cgctcacgcc ttgcatccgg tgagattgtc gcacgcggct
     1921 tgtcgcgacg aacatcaaac atagccccgg gcggcctgcc cggttccgtc tccacgggag
     1981 cgggtgacgg atacccacca gcctctccgg aggggcgggc ggggcgagcc gccccgcgct
     2041 acatcactcg catcgttccg accgctcagg cactggcttc gagggcaagg atggtttgct
     2101 tgcggtcggc gccccagcga tagccgccga ggccaccatc ggagcggacg acgcggtggc
     2161 aggggacagc gagggcggtc gggttcgcgc cgcaggcatt tgcgaccgcc cggaccgcgc
     2221 tcgggctccc gacctgggtc gcgatctgct ggtagctgcg ccgctcgccg cgcgggatgc
     2281 gtcgcagctc ggtccagacc ttgcgctgga acgcggtgcc a
//
LOCUS       DASSGX010000478         3078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1610188, whole genome shotgun sequence.
ACCESSION   DASSGX010000478 DASSGX010000000
VERSION     DASSGX010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1610188"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1207)
                     /locus_tag="VFI12_08565"
     CDS             complement(<1..1207)
                     /locus_tag="VFI12_08565"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET7056502.1"
                     /translation="MSVVRVLGLAALVVGVALPVLGQGIPKAQSPEEVGFSSVRLKRL
                     SDRINDDVKEGKIPGAVVLIARNGRIVMFDAFGYRDRDAKAPMQTDSIFRIASMSKPI
                     TSVAAMTLVEEGKMSLADPVAKYIPAFGETKVSVTKTKPDGTVELGLEPQERPMTILD
                     LMRHTSGLTYPAPGGNPVKQSYYDMKVMDANQTNAEMAAKLAKLALLYQPGTRFEYSM
                     STDVLGRVIEVVSGMPLDKFIEERIAKPLKLGDTGFDVTADKKGRGAHPQKEGPRNEV
                     PPIPDVTYRTTWKSGGAGMVSTAADYARFLQMFANGGVLDGVRVISRKTIDLMTANNL
                     PPDIKMGDDMWRLGPLEPSRQMGQGFGLGFAVRIDQGRNPLPGSPYDYHWGGVWGTYF
                     WHDPRERLYV"
     gene            complement(1261..2307)
                     /locus_tag="VFI12_08570"
     CDS             complement(1261..2307)
                     /locus_tag="VFI12_08570"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HET7056503.1"
                     /translation="MKRLLIALWLGLAGIPNAVAHVTSTGLAVLEVDGGRLSYRLTLV
                     ATEQDEEMGRMLLAAADGDAAAAARVAQAMREHARFSVAGEPCLPGRITVQGRRGDDK
                     VALEMALTCPRTTGTLVVHDEWPKVLGGHFQTVMSVRIPGQPSREFALGEERREATVE
                     LSGAATTGWLSFIGMGTEHILSGPDHLLFLLALLALTRGFWPIAKIVTGFTIAHSVTL
                     SLATLGVLDVPSRIVEPLIAATIIWVALENLLAPARTAQRWLIAALFGLIHGLGFASA
                     LTELGLPRAAMIRALIGFNVGVELGQLAFVAAVMPAVVWLAKPGRIARLPQVLSVAVA
                     AAGTFWLAERLLFA"
     gene            complement(2304..2858)
                     /locus_tag="VFI12_08575"
     CDS             complement(2304..2858)
                     /locus_tag="VFI12_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056504.1"
                     /translation="MAVAPPLIRRLAWPTAAALALLFVVGLALTGERPDAGIAQFKAS
                     GLLTAFAPDDTHEVEIAVGGKTWRFRREDVWHAVEAPAKVAPDVGGRIDAALRLLRNA
                     GPLRVLTADEVAQMPESEYALSAQSLLVRLHGPNGAMFQVRFGGRNPLGSARYSRVMG
                     VEGLPMLPTYVSEAWEQVIGEGER"
     gene            complement(2837..>3078)
                     /locus_tag="VFI12_08580"
     CDS             complement(2837..>3078)
                     /locus_tag="VFI12_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056505.1"
                     /translation="ADFASNSFFPYLANADIVLAGIAWLLHEERLPTLKPPVEVLPLV
                     TLTNAEVRWIFILTVILLPGAVACAGVGVWLWRRR"
BASE COUNT          566 a         1059 c          973 g          480 t
ORIGIN      
        1 caacgtacag gcgctcgcgc gggtcgtgcc agaagtaagt cccccagacg ccgccccagt
       61 gatagtcata cggcgagccc ggcagcgggt tccgcccctg gtcgatgcgg acggcgaatc
      121 ccaggccaaa tccctgtccc atctgccgac tgggctcgag cggacccagc cgccacatgt
      181 cgtcccccat cttgatgtcg ggagggaggt tgttcgcggt catgagatcg atggtcttgc
      241 gggagatgac gcgtaccccg tcgagcacgc cgccattggc gaacatctgc aggaaacgcg
      301 cgtaatctgc ggcggtcgag accatgcccg cgccgccgga cttccaggtc gtgcgatagg
      361 tgacatcggg aatcggcggc acctcgttcc gcggcccctc cttctgcgga tgcgcgccgc
      421 gccccttctt gtccgcggtc acgtcgaagc ccgtgtcccc cagcttgagg ggcttggcga
      481 tccgctcctc gatgaacttg tcgagcggca tgccggagac gacttcgatc acgcgaccca
      541 gcacatcggt ggacatggag tactcgaagc gcgttcccgg ctgatagagc aaggcgagct
      601 tggcgagctt ggccgccatc tcggcattgg tctggttggc gtccatcacc ttcatgtcat
      661 agtaggattg cttgacggga ttgccgcccg gtgccgggta ggtgaggccc gaggtgtgcc
      721 gcatcagatc caggatggtc atcggccgct cctgcggctc gagccccagc tcgacggtcc
      781 cgtccggctt tgtcttcgtc accgagacct tggtttcgcc gaaagccggg atgtatttcg
      841 ccaccggatc ggcgagcgac atcttcccct cctcgaccag cgtcatggcc gcgaccgagg
      901 tgatcggctt gctcatggag gcgatgcgga agatcgagtc ggtctgcatc ggcgccttgg
      961 catcacgatc gcgatagccg aaggcatcga acatgacgat tcgtccgttg cgcgcgatca
     1021 ggaccacggc tcccgggatc ttgccttcct tcacgtcgtc gttgatgcgg tcggacagcc
     1081 gcttgaggcg gacggatgag aagccgactt cctcgggcga ctgcgccttg ggaatgccct
     1141 ggcccaagac cggcaaggca acgccgacga ccaatgccgc caaaccgaga acgcgaacca
     1201 ccgacatgcc gacttctccc ccgatgttcg gcacatcaaa gcatcgatcg tcctcgccag
     1261 tcaggcgaaa aggagccgtt ctgccaacca gaaggtgccg gcagcagcga cggccaccga
     1321 caaaacctgc ggaagacggg caatgcggcc cggtttcgcc agccaaacca ccgccggcat
     1381 gacggcggca acaaaggcga gctgccccaa ctcgacgccg acattgaacc cgatcagcgc
     1441 gcggatcatc gccgcgcgcg gcaggccgag ctcggtcagg gcgcttgcga agccgagccc
     1501 gtgtatcagc ccgaaaagag cggcgatcag ccagcgttgc gccgttcggg ccggcgccag
     1561 caggttctcc agcgccaccc agatgatcgt cgcggcgatc agcggctcca cgatccgcga
     1621 cggcacgtcg aggactccca atgtggccag gctcaacgtg acgctgtgcg caatggtgaa
     1681 gcccgtgacg atcttggcga tcggccagaa gccgcgcgtc aacgcgagca gcgccagcag
     1741 gaacagcaga tgatccggcc cgctcaggat gtgctcggtg cccatgccga tgaagctcag
     1801 ccatccggtc gtcgcggcgc ccgaaagctc gaccgtggcc tcgcgtcgct cttcgccgag
     1861 tgcgaattcg cgcgacggtt ggccggggat gcgcacactc atcaccgtct ggaagtggcc
     1921 gcccaagacc ttcggccact cgtcgtggac aacgagcgtc cctgtcgttc gcggacaggt
     1981 gagcgccatc tccagcgcca ccttgtcgtc gccgcgtcgg ccttgcaccg tgatccggcc
     2041 cggcaagcac ggctcaccgg cgaccgagaa tcgggcatgc tcgcgcatgg cctgcgcaac
     2101 gcgggcagcc gcagcggcat ccccatcggc ggcagcgagc agcatccgtc ccatctcctc
     2161 gtcctgttcc gtcgcgacca gggtgagacg gtaggacagg cggccgccat ccacctccag
     2221 caccgcaagt ccggtgctgg tgacatgcgc gacggcattg gggatgccag caagaccgag
     2281 ccacagagcg atcagcaacc gcttcatcgc tcgccttccc cgatcacctg ctcccacgcc
     2341 tccgacacgt aggtaggaag catcggcagt ccctcgacac ccatgacgcg cgaatatcgt
     2401 gccgagccca ggggattgcg gccgccaaac cggacctgga acatggcgcc gttcggaccg
     2461 tgcagccgga cgagcaggct ctgcgcacta agcgcatatt ccgactcggg catctgggcc
     2521 acttcatccg ccgtcaggac gcgcaacgga ccggcattgc gcaacaggcg gagtgcagca
     2581 tcgattcgtc caccgacatc aggcgccacc ttcgctggcg cctcgaccgc gtgccagaca
     2641 tcctcgcgcc gaaagcgcca ggtcttgccg ccgacggcga tctcgacctc gtgcgtatcg
     2701 tccggcgcga aggcggtcag caagcctgac gccttgaatt gagcgatccc tgcgtcgggc
     2761 cgctcgcctg tgagggcaag tccaaccacg aaaagaagcg ccagggccgc cgccgtgggc
     2821 caggccagtc gccggatcag cggcggcgcc acagccatac ccccaccccg gcgcaggcga
     2881 cggcgccggg cagcaggatc accgtcagga tgaagatcca gcgcacctcg gcattggtca
     2941 gcgtgaccag cggcagcacc tcgaccggcg gcttgagcgt cggcaaccgc tcctcgtgca
     3001 acagccaggc gatgccggcc aagacgatgt ccgcgttggc gagataaggg aagaacgagt
     3061 tgctggcgaa atcagcat
//
LOCUS       DASSGX010000479         2036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_519725, whole genome shotgun sequence.
ACCESSION   DASSGX010000479 DASSGX010000000
VERSION     DASSGX010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519725"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VFI12_08585"
     CDS             <1..283
                     /locus_tag="VFI12_08585"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HET7056506.1"
                     /translation="TDLDAILRESGVEHLVTCGMQTDYCVDSATRSAMHRGFNVTLAT
                     DAHSTWDNGVLTAEQIVAHHNKTLPDLPGPGTDIFGRASAEIEFAPAGA"
     gene            284..859
                     /locus_tag="VFI12_08590"
     CDS             284..859
                     /locus_tag="VFI12_08590"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HET7056507.1"
                     /translation="MSADASSLPTALLVIDVQEAMFGPGQQPPVFRADETVANIATLL
                     TKARSAGARVIYIQHHDQIDPALQPGQSGFAIRAAIAPEPGETVLVKRICDTFTGTNL
                     VQVLREAGIERLVTCGIQSDFCVDAATRGAMNRGFSVILASDAHTTWDNGVLSAEQII
                     AHVNATLPNMSGPGERTQVKPTAAITFRVAT"
     gene            901..1125
                     /locus_tag="VFI12_08595"
     CDS             901..1125
                     /locus_tag="VFI12_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056508.1"
                     /translation="MVETKIHISERQLAELEAAEQREDRPRDEIVQDALERYFEEHQR
                     RLPQSIGIIEDHEVNSTNFRDWLRENWHPE"
     gene            1122..1523
                     /locus_tag="VFI12_08600"
     CDS             1122..1523
                     /locus_tag="VFI12_08600"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HET7056509.1"
                     /translation="MITLDTSAIYAYLNRFDVNHQAVSRTLHNEAGPLVVPVSILSEI
                     AYLLERHGQNPLRAFLGDIEREEYLLDCGAGDLQRIGELIRRYDDLPLGFADAAVIAC
                     AERRGRRVLTYDRRHFSVVSREGSIILAGVD"
     gene            complement(1520..>2036)
                     /locus_tag="VFI12_08605"
     CDS             complement(1520..>2036)
                     /locus_tag="VFI12_08605"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET7056510.1"
                     /translation="PNVSAYWECPEPGRTVIDPRGHGPMGYVIPAAMGVSLADPERPV
                     VGLCGDGSFAMACGELETAHRLNLPVLYVQFTNNSLGWIKMLQHLYLGQRYFGVDPGP
                     MNYVGVAQAMGLEAIRVNDLDQLRGAVREWLTHRRPTFIDVPVPDQIALTPPVAPWQA
                     ALEGASGRPVY"
BASE COUNT          383 a          651 c          652 g          350 t
ORIGIN      
        1 aacggacctg gacgcgatcc tgcgcgagtc cggcgtggag catctggtca cctgtggcat
       61 gcagaccgac tactgcgtcg attcggcaac ccgatcggcc atgcaccggg gattcaacgt
      121 cacgctggca accgatgcgc attcgacgtg ggataacggc gtgctgaccg ccgagcagat
      181 cgtcgcccat cacaacaaga ccctgccgga cctgccgggt cccggcaccg acatcttcgg
      241 cagggcgtcg gccgagatcg agttcgcccc ggccggtgca tagatgagcg cggacgcgtc
      301 atcactgccg accgcgctgc tggtgatcga tgtccaggag gcgatgttcg gtccgggcca
      361 gcaaccgccg gtcttccggg cggacgagac cgtcgccaac atcgccacgc tgctcaccaa
      421 ggccagatcg gccggcgcga gggtgatcta catccagcac catgaccaga tcgacccggc
      481 attgcaaccg ggtcagtctg gcttcgcgat ccgggcggcg attgcgccgg agccgggcga
      541 gacggtcctc gtcaagcgca tctgcgacac cttcacgggg acgaacctgg tgcaggtgct
      601 gcgtgaggcg gggatcgagc ggctcgtgac ctgcggtatc cagtcggact tctgcgtgga
      661 cgcggcaacg cgcggcgcga tgaaccgcgg cttctccgtg atcctggcca gcgacgccca
      721 caccacctgg gacaacggcg tgctgagcgc ggagcagatc atcgcccacg tcaacgccac
      781 gctccctaac atgtccggcc ccggcgagcg aacccaggtc aaaccgacgg ccgcaatcac
      841 gttccgggta gctacctgat gccacagttc cgggctttgg cgatactgta agatcaagct
      901 atggtcgaga cgaagattca catttcagag cgtcaattgg ccgaactcga ggcggctgag
      961 caacgggagg accgtcccag ggacgagatc gttcaggatg cgctggaacg ctatttcgaa
     1021 gagcaccaac ggcgactgcc ccaatcgatc ggaataatcg aggaccacga agtcaactcg
     1081 accaatttca gagactggct gcgggagaac tggcaccccg agtgattacc ctcgatacgt
     1141 cggccattta cgcatatctg aaccgattcg atgtcaatca ccaggccgtc agccgcacgc
     1201 tgcataatga ggcgggaccc cttgtcgttc cggtctcaat tctctctgag atcgcatacc
     1261 tgcttgagcg gcacggacag aatccccttc gtgcatttct cggcgatatt gaacgtgagg
     1321 agtacctact cgattgcggt gccggcgacc ttcagcgtat cggggagttg atccgccgat
     1381 atgacgatct cccgctcggc ttcgcggatg ccgcagtgat tgcttgcgcg gagcgacgag
     1441 gccgcagagt cctcacctac gaccgccgtc atttttccgt cgtatcacga gaaggtagca
     1501 tcatccttgc cggtgtcgat taatacaccg ggcggccgga ggctccctcg agggcggctt
     1561 gccagggggc gacgggtggg gtgagcgcga tctggtcggg gacggggacg tcgatgaagg
     1621 ttgggcggcg gtgggtgagc cactcgcgga cggccccgcg gagctggtcg aggtcgttga
     1681 ctcggatcgc ctccagcccc atcgcctggg cgacgccgac gtagttcatc ggccccggat
     1741 cgacgccaaa gtagcgctga ccgaggtaca agtgctggag catcttgatc cagccaagtg
     1801 agttgttcgt gaactggacg tagaggaccg gcagattgag ccggtgcgcc gtttccagct
     1861 ccccgcacgc catcgcgaag ctgccgtcgc cgcatagccc cacaaccggg cgctctggat
     1921 cggcgagcga cacccccatc gccgccggga tgacgtaccc catcggcccg tgcccgcgcg
     1981 ggtcgatcac ggtccggccc ggttccgggc attcccagta ggcggagacg ttcggc
//
LOCUS       DASSGX010000480         6019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_301298, whole genome shotgun sequence.
ACCESSION   DASSGX010000480 DASSGX010000000
VERSION     DASSGX010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6019
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301298"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..1584)
                     /locus_tag="VFI12_08610"
     CDS             complement(16..1584)
                     /locus_tag="VFI12_08610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HET7056511.1"
                     /translation="MRDVELILILLAIIAVLVSLAARLAIPYPILLVLGGLGLGFVPG
                     LPAIELDPHIVFVLFLPPLLYWDAASTSWRDFRASTRTITLLAFGLVVATTVGVGVLT
                     HALLDLPWAAAFILGAVVSSTDPVAASAVMRRLGVPPKVITIVQDESLVNDATALVVF
                     SVAITVLKTGEFSLGRAALDFVFVTVGGVVVGLAAGWLLHRLQHQIDEARVSSVLRLM
                     TPFVVYILAEQVHVSGILAVVVTGLYAGRKAPTDLSSEERLGGAVIWQLVTFIINGLV
                     FILIGLQFRTILNNIDETSTRSLVQDALLVSLAVIAIRLIWVYLAGAGIRLLPQFLTG
                     REAELDAREMTIVGWSGMRGVVALATALSVPRVTESGIFGERPLVLFLTFSVILVTLV
                     GQGLTLPFVITALGVTRGDRSEREEQRAREAMTNAALRRLDALSQSSLSPEMYRMLQR
                     YYRDQLTRSRQPSAPGDESWVQNIRDVRRELLAAEERALVRLRDQEVISDAVLRNLQR
                     ELDIEGLHLDTRVI"
     gene            complement(1669..2709)
                     /locus_tag="VFI12_08615"
     CDS             complement(1669..2709)
                     /locus_tag="VFI12_08615"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET7056512.1"
                     /translation="MTDTRLEPISDVQSQEQERDRRDAIRQSRVIVALFLAYFLIGMA
                     LTLIRGGSFLTPDRIAVLMLAGAILLGQGKAFVRDWGPFLLLLFGYELMRGVADNMTS
                     LGSFTAADHGRVLVKPLIDADKALFFGHIPTLWLQDRLYTPGVVHWYDTGAALIYLLH
                     FVLPLAFGFALWLRDRAAFRRFVLMLLVMSYGAFIFFLLVPAAPPWLAAEWGYIDNVD
                     RPSDEAYKSFLPHRWRNYDTFRLWTRASPNPVAALPSLHAAFPWLVLLVACATFRWWG
                     LLLVPYNLALWFSVVYLSQHWAVDVLAGIAWATAIWLFFTWILVWRARGREPVPEHTP
                     RIEPPLTERAQT"
     gene            2776..5322
                     /locus_tag="VFI12_08620"
     CDS             2776..5322
                     /locus_tag="VFI12_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056513.1"
                     /translation="MIASDATLGVPAPHPASLDTASLEREFPRSRDAVCLGVLLALAL
                     GIGIVWMPFSLYRLDNLTFYLPWYTELGRSLRSFDIPGWLPTTLSGAPMAGDPQSGWG
                     YLPAMVIMTAFPSLAGYKLFLLFHILAAALTSYLYARNLGIGPIGALTTGLVFTFGNF
                     LERTSCCTVHMQVAIWIPAVFLCIDLSQRAVTRTARIGWLLLAGLGASQIAAGWVGQG
                     AYYGGLAVIGYMVYRCLISPRRPLRFRQRVVWLISSSVVMGVIAGSAALTAIVPRLDV
                     VSRSTLSDLYDDGAGAREEIGWSISTLAERALGQPVREGRWYLGVVGLALAILAPLVV
                     RRRRDVTFFALYTLIVLSLIVKGSPMIALFNLLPQFRSLHSHSPDRIYIVLFIGPALL
                     AGFLVDALDRRRWRSLSPWGLMATVELPIVLIVAALILVQANERAWLPWYHIVLVIAV
                     CIAVSVALVRVRPWSTTAAIVAVIAILLIDTPGHNAWGRITDRSSRSTASRVIDGYLE
                     PNGAAQWLQQRRDEGEIFRYFGFDLAALTIPDRRQTYAVGHYRSGTSSILINNRGVQF
                     GLDDIQGYNPVQVSRYVTYFRYLNGQSQSYHTSNVLENGIDSPLLDLLNVRYIVVPAQ
                     IPPGRPDLFHLAQIYPTVYLDGTNRILENPDLLPRTWIVHETVKTRTGQILPQFIDGS
                     VDPSLTALIDGDAPTLSPIPAGATESVEITHRENDEIRLRVNAASRGMVILSEVWDPG
                     WTATVDGEKARVYRADFLFRGVLVGPGEHEIVLRYPATDVKRTLAFYLIPLAAFALLG
                     LVSARNRYMSGRRAPQDEERVTDPLVVAAPDADTDVWTDWPEPQRDPPES"
     gene            5365..>6019
                     /locus_tag="VFI12_08625"
     CDS             5365..>6019
                     /locus_tag="VFI12_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056514.1"
                     /translation="MFAMQESHTAPITTAAPWVAVPHDRAVEGVTPRSRDLIGLGALL
                     VVVLAVLVVNFWSRYNLYRLDIVTFYIPWYEHLGERLRDLDIPGWMPYAMSGSPFAAD
                     PQSGWGYLPAMVIFTIAPSLGGYIAFVGFHVVLAAAGTYAYARVIGINPAGAFAAGTT
                     FTLGNFMERTACCTVHMQVAVWIPAIFLCYELSRRARTGASRFGWLVLAGFGTGQMVA
                     "
BASE COUNT         1037 a         1983 c         1994 g         1005 t
ORIGIN      
        1 gcggtgccag cctgatcaga tcacgcgcgt atcgaggtgc agcccctcaa tgtcgagctc
       61 gcgttgcaaa ttccggagca ccgcatcgct gatcacctcc tgatcgcgca atcgcaccag
      121 cgcccgctcc tcggccgcca acagctcccg gcggacgtcc cgaatgtttt ggacccagga
      181 ctcatcgccg ggcgcggagg gctgccggct gcgggtcagc tggtcccggt aatagcgttg
      241 aagcatccga tacatctctg gggacaggct cgattgcgag agggcgtcga ggcggcggag
      301 ggcggcgttg gtcattgcct cacgcgcccg ctgctcctcg cgttccgagc ggtcgccgcg
      361 ggtcacgccg agagcggtga tgacgaaggg gagggtcagc ccctgcccga ccagtgtgac
      421 caggatcacc gagaaggtca ggaaaagcac gagcggccgt tcaccgaaga tgccggactc
      481 ggtcacgcgg ggaaccgaga gcgccgttgc aagcgccacc acaccccgca tccccgacca
      541 gccgacgatc gtcatctcgc gggcatcgag ctccgcctcg cggccggtga ggaattgtgg
      601 aagcaggcga atccccgcgc cggccaggta gacccagatt aaccggatgg caatgacggc
      661 caacgagacg agcagagcat cctggacgag gctccgggtg gaggtctcgt cgatattatt
      721 caggatggtc cgaaactgga ggccaatcag gatgaagacg agcccgttga tgatgaaggt
      781 gaccaattgc cagatgacgg caccgcccaa ccgttcctcg gacgagaggt cggtcggcgc
      841 cttgcggccg gcatagaggc cggtcacgac gacggcgagg atcccggaga cgtggacctg
      901 ctccgccagg atgtagacga cgaagggcgt catcagccgc agaacgctgg agacgcgcgc
      961 ctcgtcaatc tggtgctgca accggtggag cagccagccg gctgccagcc cgaccaccac
     1021 gccgccgacg gtcacgaaga cgaagtcgag ggccgcccgg ccaagagaga actcgccggt
     1081 ctttagcacc gtgatcgcaa ccgaaaagac aacgagcgcc gtcgcatcgt tgaccaggct
     1141 ctcgtcctgg acgatcgtaa tcaccttggg cggcactccg agccgccgca tgacggcgct
     1201 ggccgcgaca ggatcggttg aggagacgac tgcgccgagg atgaacgcgg ccgcccacgg
     1261 caggtccagc agggcgtgcg tcaacacgcc gaccccgaca gtggtggcga ccacaagacc
     1321 gaaggcgagc agcgtaatcg tccgggtgct cgcccggaaa tcccgccacg aggtcgaggc
     1381 ggcgtcccag tagagcagcg gcggcaagaa gaggacgaag acgatgtgcg ggtcgagctc
     1441 gattgccggt aaaccgggca cgaaaccgag accgagacca ccgagcacga gcaggatggg
     1501 ataggggatc gcaagccgag cggccagcga caccagcacg gcgatgatcg ccagcaggat
     1561 caggatgagt tcgacgtcgc gcatcgatcg ccttcgaggt gtcggatcat ggtcctggtg
     1621 gaaccggcgc aatcctgcca gagcacgtgc gggcggcgtt ctcccgcgct acgtctgggc
     1681 gcgctcggtc agtggcggct cgatgcgggg ggtgtgctct ggaaccggct cccggccccg
     1741 ggcacgccaa acgaggatcc acgtgaaaaa cagccagatc gccgtcgccc aggcgatccc
     1801 ggccaggaca tcgactgccc aatgctgcga gaggtagacc accgagaacc agagcgccag
     1861 gttgtacggg acgaggagca ggccccacca gcggaaggtc gcgcacgcca ccagcagcac
     1921 cagccagggg aaggcggcgt ggagcgaggg cagcgcggcg acgggattcg gactggcccg
     1981 cgtccagagc cggaaagtgt cgtagttccg ccagcgatgc ggcaggaagc tcttgtacgc
     2041 ctcgtcggag gggcggtcga cattgtcgat gtacccccac tcggcggcca gccatggcgg
     2101 ggcagcgggc accagaagga agaagatgaa ggcgccgtag ctcatcacca gcagcatgag
     2161 cacgaagcga cggaaagcgg cccggtcccg cagccagagg gcgaagccga acgccagcgg
     2221 cagcacgaag tggagcaagt agatcagcgc cgcgccggtg tcgtaccagt ggacaacgcc
     2281 tggcgtgtag agccggtcct gcagccagag cgtcgggata tggccgaaga agagcgcctt
     2341 gtcggcatcg atgagtggct tgacgaggac ccgaccgtgg tcggcggcag tgaaactgcc
     2401 gaggctggtc atattgtcgg ccacgccacg catcagctcg taaccgaaga gcaggagcag
     2461 gaagggcccc caatcgcgca cgaaggcctt gccctgtccg agcaggatcg ccccggccag
     2521 catcaggacg gcgatccggt ccggtgtcag gaaggaaccg ccccggatca gggtgagcgc
     2581 catgccgatc aggaaatagg cgaggaaaag cgcgacgatg acgcggctct gccggatcgc
     2641 gtcgcggcga tcccgctcct gttcctgtga ttgcacgtcg gagattggtt cgaggcgcgt
     2701 atcggtcatc gggcacaaca atactgtccc gggccgcccg gatgcgaatc gtttttatga
     2761 cgtattatcc ggccgatgat tgcctccgat gccaccctgg gcgtgccggc gccccaccca
     2821 gcttcccttg acacggcatc cctggagcgg gaattcccgc gctcgcgcga tgccgtctgc
     2881 cttggcgtgc tgctcgcgct cgcacttggg atcggcatcg tctggatgcc gttctccctc
     2941 taccggctcg ataacctgac cttctacctg ccttggtaca ccgagcttgg ccggagcctg
     3001 cgctcgttcg acatcccggg ctggctgcca acgacgctct ccggcgcgcc catggccggc
     3061 gatccccaat cgggctgggg gtacctcccg gccatggtga tcatgacggc attcccgtcg
     3121 ttggccgggt acaagctgtt cctgctcttc cacatcctcg ccgcggcgct cacctcctat
     3181 ctctacgccc gcaacctcgg gatcggcccg atcggcgcgc tgacgaccgg attggtcttc
     3241 accttcggta atttcctgga gcgaacctcc tgttgcactg tccatatgca ggtcgcgatc
     3301 tggatcccgg ccgtcttcct ctgcatcgat ctcagccagc gggcggtgac gcgcacggcc
     3361 cggatcggct ggctgctgct ggcgggactg ggggccagcc agatcgcggc aggctgggtc
     3421 gggcagggcg cctattacgg cgggctggcg gtgatcggct acatggtcta tcgctgcctg
     3481 atcagccccc gccgcccgct ccggtttcgc cagcgcgtgg tctggttgat cagcagcagc
     3541 gtggtgatgg gcgtcatcgc cgggtcggcc gcgctgaccg cgatcgtgcc ccggctcgat
     3601 gtcgtcagcc gctccacgtt gtccgacctc tacgacgatg gcgcgggcgc gcgcgaggag
     3661 atcggctggt cgatttcgac cctggccgag cgcgcgctcg gtcagccagt tcgcgagggg
     3721 cgctggtatc tcggggtcgt tggcctggcg ctggcgattc tcgcgccgct cgtggtccgg
     3781 cggcgacggg acgtaacctt tttcgccctc tacacgctga tcgtgcttag cctgatcgtc
     3841 aagggctcgc cgatgattgc cctgttcaat ctgctgccgc agtttcgctc gctccattcg
     3901 cactcgccgg accggatcta catcgttctg ttcatcgggc cggcgctcct ggccgggttc
     3961 ctggtcgatg cgctggaccg ccggcgatgg cggtcgctca gcccgtgggg gctgatggcg
     4021 acggttgagt tgccgatcgt gctaatcgtg gcggcgctga tcctcgtgca agcgaacgag
     4081 cgggcgtggc tgccctggta ccacatcgtg ctggtgatcg cggtctgtat tgccgtgagt
     4141 gtcgccctgg ttcgggtccg gccatggtcg acgaccgcgg cgatcgtcgc cgtcatcgcg
     4201 atcctgctga tcgacacgcc gggccacaac gcgtggggcc ggatcacgga ccggtcgtcc
     4261 cgctcgactg cgtcacgggt tatcgatggt tatctcgagc ccaacggcgc ggcccagtgg
     4321 ctccagcagc ggcgggacga gggcgagatc ttccggtact tcggcttcga cctcgcggcc
     4381 ctgaccatcc cggaccgccg ccagacgtac gcggtcggtc attatcgatc cgggacgagc
     4441 tcgatcctaa tcaacaaccg gggcgtgcag ttcggcctgg acgacatcca gggatacaac
     4501 ccggttcagg tcagccgcta tgtcacctat ttccggtacc tgaacggcca gtcgcagagc
     4561 tatcacacct cgaacgtgct cgaaaacggg atcgattcac cgctgctgga cctgctcaat
     4621 gtccgctaca tcgtggtgcc cgcccagatc ccacccggcc gaccggacct gttccacctc
     4681 gcgcagatct acccgaccgt ctacctcgac ggcacgaacc ggatcctcga gaacccggat
     4741 ttgctgccga ggacctggat cgtgcacgaa accgtcaaga caaggaccgg acagatcctg
     4801 ccgcaattca tcgacggctc ggtcgacccg agcttgacgg cgttgatcga cggcgacgcg
     4861 ccgaccttga gtcctattcc ggccggggcg acggaatcag tcgagatcac gcaccgtgag
     4921 aacgacgaga tccggctacg ggtgaatgcc gcttcgcgcg ggatggtgat cctcagcgaa
     4981 gtctgggatc cgggatggac ggccaccgtc gatggcgaaa aggcaagagt ctaccgggcc
     5041 gatttcctgt tccgcggcgt tctggtcggg ccgggcgagc acgagatcgt gctgcgctac
     5101 ccggccaccg acgtcaagcg gacactcgcc ttctacctga tcccgctcgc cgctttcgcg
     5161 ttgctcggcc tcgtttccgc ccgcaaccgc tacatgtccg gccgccgggc ccctcaggat
     5221 gaagaacggg tgaccgatcc cctggttgtg gcggcacccg acgcggatac cgacgtgtgg
     5281 accgattggc ccgagccgca acgcgaccct cccgaatcct gacgtcgccc cgagccgcct
     5341 gctggtctca caaggcgcgt atccttgttt gccatgcagg agagtcacac cgcgccaatt
     5401 actacggccg cgccatgggt cgcggtcccg cacgaccggg ccgttgaagg cgttacgccc
     5461 cgctcccggg atctgatcgg gctcggcgcg ctgctggttg tcgtcctggc ggtcctcgtg
     5521 gtcaacttct ggagccggta caacctctac cggctcgata tcgttacctt ctacatcccc
     5581 tggtacgagc acctcggcga gcgactgcgc gacctcgata tccccggctg gatgccttat
     5641 gccatgtccg gctcgccctt cgcggccgat ccgcagtcgg gctggggcta cctgccggcg
     5701 atggtcatct tcacgatcgc gccgtccctc ggcgggtaca tcgccttcgt cggatttcac
     5761 gtcgtgctcg ctgcggccgg gacgtatgcc tacgcacggg tgatcggcat caacccggcc
     5821 ggggcgttcg cggccggaac gacctttacg ctcggcaact ttatggagcg gaccgcctgc
     5881 tgcacggtcc acatgcaggt cgcggtctgg atcccggcga tcttcctctg ctacgagctg
     5941 agccggcggg cgcggacagg agcgagccgg ttcggctggc tggtcctggc cgggttcggc
     6001 acggggcaga tggtcgccg
//
LOCUS       DASSGX010000481         2619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_412528, whole genome shotgun sequence.
ACCESSION   DASSGX010000481 DASSGX010000000
VERSION     DASSGX010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412528"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            6..161
                     /locus_tag="VFI12_08630"
     CDS             6..161
                     /locus_tag="VFI12_08630"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HET7056515.1"
                     /translation="MAGPSATNPILYGGSVNESNAGALLAADGVSGLLVGGASLDPAG
                     WARICST"
     gene            256..654
                     /gene="secG"
                     /locus_tag="VFI12_08635"
     CDS             256..654
                     /gene="secG"
                     /locus_tag="VFI12_08635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HET7056516.1"
                     /translation="MYTFLLILLVLDSVVLIAVILMQAGQGGGMAATFGGASSSAGAI
                     FGTRQAGNLLTKTSWWGGALFLLLSFALSLGSTRSSAPKSVLDQAFPTSTAPVAPPST
                     TPPAGLNLSTPPNSASTTPPATPPAGTKKP"
     gene            655..1803
                     /gene="carA"
                     /locus_tag="VFI12_08640"
                     /pseudo
     CDS             655..1803
                     /gene="carA"
                     /locus_tag="VFI12_08640"
                     /inference="COORDINATES: protein motif:HMM:NF013178.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
     gene            1883..2227
                     /locus_tag="VFI12_08645"
     CDS             1883..2227
                     /locus_tag="VFI12_08645"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET7056517.1"
                     /translation="MTKADLVERVTAQISRTAGPMISKKDCARVVDAFLDAIKESLQT
                     QKNIEVRGFGTFKIRHRKTRMARNPRTGSPVEVSARPVPVFKPSKELRAMVAGIDIAD
                     LLDDEEEELSPA"
     gene            2319..2558
                     /locus_tag="VFI12_08650"
     CDS             2319..2558
                     /locus_tag="VFI12_08650"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HET7056518.1"
                     /translation="MTVTVLLFASYAESLGKSSVTLDLPEGAKVADAVARIQASAAGA
                     LPPRPLVAINQSYAALSDRLTAGDELAIIPPVAGG"
BASE COUNT          497 a          828 c          808 g          486 t
ORIGIN      
        1 ctcagttggc gggaccgtcc gcgacgaatc cgattctcta cggtgggagc gtaaacgaat
       61 cgaacgccgg cgctctgctc gcggcggacg gcgtgtcggg cctgctcgtc ggcggagcaa
      121 gtctcgaccc ggcgggctgg gctcgcatct gctcgacttg accgaacgct gtaagtcctg
      181 tgatatttag gggttatccg cgatcaacgc gggtactacc aagatcgccg tcgaccgtca
      241 gccgataacc tcgccgtgta cacgttcctc ctgatcctcc tcgttctcga cagcgtcgtc
      301 ctgatcgccg tcattctcat gcaggccggt cagggtggtg gcatggccgc gaccttcggg
      361 ggtgcgagca gctcggccgg ggcgattttc ggaacgcgac aggccggcaa ccttctgacg
      421 aagacgagct ggtggggcgg tgcgctgttc ctcctgctct cgtttgccct ttcgctcggc
      481 tcgacgcgct ccagcgcgcc aaagtccgtt ctcgatcagg cctttccgac gtcgaccgcg
      541 ccggttgcgc caccgtcgac gacgccgccc gctggtctca acctgagcac tccgccgaac
      601 tccgcctcaa cgacgccgcc agcgacgcct ccggcaggca cgaagaagcc atagatggct
      661 gatcggtcgc gcctgacggc agcaccgggg ttcctcctcc tcgaggacgg aacccttttt
      721 cgtggcaggc tcgccggcgc agcggacaat actcccgcgg tcgccgaagt cgtgttcacc
      781 acgaacatga gcggctacca ggaagtgttc accgatccgt cgtatcaggg gcagatcgtc
      841 gtgatgacag cgccgatgat cggcaactac ggcgtgaacg ccgacgaccc ggagtcgaaa
      901 ggcgctcagg tcgccggcgt gattgttcgc gagctctcga agagttactc gaactagcgt
      961 gcacagggcg acctgaatga ctatctgcga aaggcggaaa ttcccgtcct ggaagaggtt
     1021 gatacgcgtc gccttacccg tcacctgagg acggttggat cattgcgggg agtcatcggt
     1081 gcgggttcgg cgccggataa ggcagcgctc gatgcgctgg gatcatgccc ctcgatggag
     1141 ggactggatc tcgcgaccgt cgtatcgaca aaggagcgct acagcttcgg caaccctcag
     1201 gcgaagagcc atatcgtcgc gtacgactat ggcatcaagc agaacatcct gcgtctcttc
     1261 gccgagcagg actgtcacat caccgtcgtg ccgtccgaga cgtcggccgc cgaagtgctg
     1321 gcgctgaagc ccgacggcgt tttcctctcg aacggacctg gcgaccccgc ggccgtcgac
     1381 tacgcgccag ccgcggtgaa atcgattgcg ggagctggcg ttccaatctt cgggatctgt
     1441 ctcggtcacc agatcctcgc cctgacctac ggggcggaaa cgacgaagat gccctacggt
     1501 caccgcggcg gaaatcatcc ggtgaaagag gtcggctcgg gcaaagtcct gatcacgtcg
     1561 cagaatcacg gatttgcggt aaaaggctcg gaatccgaga tcccgggcgc gccagaactg
     1621 gaggtcacgc acgtcaacct caacgacggc acgatcgaag gcctgaagca ccggactcaa
     1681 ccggtctttg cggtgcagta tcatccggag gccgctccgg gacctcatga tgcccgtccg
     1741 ctgttcgacg agttcatgga aacggtcaga aaacggagtg gtcgaagtgg cccaaactct
     1801 tgacacgtaa gagttaacgc gcctaagttc ggttacacgg cctacccctc acccgggccg
     1861 tatcgaacct gagggacccc ggatgacaaa agccgatctc gttgagagag tgaccgccca
     1921 gatttcacgg acggccggcc cgatgatctc caagaaggac tgcgcgcgag ttgtcgacgc
     1981 gtttcttgat gcaataaagg aatcgctgca aacccagaag aacattgaag ttcggggttt
     2041 cggcaccttc aagatccggc atcgcaagac gcgcatggcg cgtaaccctc gaactggttc
     2101 ccccgtcgaa gtctccgccc gaccggtgcc cgttttcaag ccgtcgaagg agctccgggc
     2161 gatggtcgcc ggaatcgaca tcgccgatct gctcgacgac gaggaagagg agctttcacc
     2221 agcgtaggca gtcggcgaat cgcgagcccg ggccatatgg cgccgggttt ttctttcccg
     2281 aggcagcacg cggtgaattc cactatcctg ttagaagcat gacagttacg gtgcttctct
     2341 tcgcgtccta tgccgagtcg ctcggcaagt cgtccgtcac cctcgatctt cccgaagggg
     2401 cgaaggtcgc tgatgcggtc gcacgcattc aggcgagcgc ggccggagct ctccctccaa
     2461 gacccctcgt cgccatcaat cagtcgtacg ccgcgctcag cgaccggctt acagccggtg
     2521 acgagctggc gatcattcca ccggtggctg gtggatgagg agggcggcgg ttgtctctca
     2581 ccccatcgat gcggcggccg ttctcgccga ggtcgaatc
//
LOCUS       DASSGX010000482         2248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1502620, whole genome shotgun sequence.
ACCESSION   DASSGX010000482 DASSGX010000000
VERSION     DASSGX010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502620"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VFI12_08655"
     CDS             complement(<1..462)
                     /locus_tag="VFI12_08655"
                     /inference="COORDINATES: protein motif:HMM:NF024804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HET7056519.1"
                     /translation="MNERSVQEAASSPALSTRALDNLPATRRKILEHLKREDDATSDA
                     IAVALGITASAARQHLSALTADGLIQFERERSGPGRPMHRYSLTTAGDALFPRNYVDL
                     TNELLGYVEDEDAELLARIFDKRGLARLAKARGRVDGLTFRDKVAMVAQILD"
     gene            583..831
                     /locus_tag="VFI12_08660"
     CDS             583..831
                     /locus_tag="VFI12_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056520.1"
                     /translation="MTMHDPLWTAGARVRERAEAYAMERNLMSQLQRPSVWSRFEVGI
                     GSLLIALGDRTRALGCRLADACGCADAPAQTGSVTGGR"
     gene            828..2087
                     /locus_tag="VFI12_08665"
     CDS             828..2087
                     /locus_tag="VFI12_08665"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7056521.1"
                     /translation="MSVAEVAIQPNLMERLGLPKLDRPGTGSLIAATWIDALGRGLFV
                     YFYLLYLTKEVGFNLNTAGAVLSVVTAFGLAVTPIAGSLVDRVGSKQMLVASQITCAI
                     GYAGLLLIPDSVPLLLLTAGMITVGECIFWVGYPNLVSQIADPEHRDRWFAFMGMSRT
                     AGFGLGGLIASGVIAIVGTHGYKVLLGANVCTFAIAATIILIRVPRVRLSMSTREHGG
                     WMAVIRDRRIMFLAAAHGFGVLTILIVFQGLPLYVVDELGLPGWVPGLLLGVNTAILA
                     AGQSIGLRIVSGWRRTRIYVLCAAIWLVGAGLFALGEFIPAGIVIPYLFLAMIIASGG
                     EVFHLPQNGGAPTALAPEALRGRYLALFSLVWSVAGIASPTLVSVLLSINGILLWVGM
                     AGAAVITALIALASEPIIDPNVQRMPV"
BASE COUNT          331 a          743 c          751 g          423 t
ORIGIN      
        1 gtcgaggatc tgcgcgacca tcgcgacctt gtcccgaaac gtgagtccat cgacccggcc
       61 ccgtgccttc gcgagccggg ccaagccgcg cttgtcgaag atccgggcca atagttcggc
      121 atcctcgtcc tcgacatagc cgagcagctc gttagtcagg tcgacgtagt tgcgcgggaa
      181 gagggcgtct ccggcggtcg tcagggagta gcgatgcatc ggccggccgg gaccgctccg
      241 ctcccgctcg aactggatca ggccgtcagc ggtcagcgcc gagagatgct ggcgggcagc
      301 gctggcggta atgccgagcg cgacggcaat cgcgtccgat gtcgcgtcgt cctcccgctt
      361 gaggtgctcg aggatcttgc ggcgggtggc gggcaggttg tcgagggcgc gcgtggagag
      421 cgccggtgac gatgccgctt cctggacact ccgctcgttc atgccgcgca gcatagcaca
      481 ggtaaaacaa ggaaacgctt gcgtaaatgg gaatggttcg ctactctggc gcagtcccgg
      541 tccccggcgg accgaatcat cacgcacact gaggtgagta ccatgacgat gcatgatccg
      601 ctctggacgg ccggcgcgcg cgtccgggag cgtgccgaag cgtatgccat ggagcgtaat
      661 ctgatgagcc aacttcagcg gccgagcgtc tggtcccggt ttgaggtcgg gatcggctcg
      721 ctcctgatcg cgctgggcga ccggacacgg gcgctcggtt gccgcctggc cgatgcctgc
      781 ggttgcgcgg atgcgccggc ccaaacagga tcggtaacgg gcggccggtg agcgtcgcgg
      841 aggttgccat tcaaccgaac ctgatggaac gactcgggct gccgaaactg gaccggcccg
      901 ggaccggctc gctgatcgcc gcgacctgga tcgatgccct cggtcgcggc ctcttcgtct
      961 acttctatct cctctacctg accaaggagg tcgggttcaa cctgaacacg gccggtgcgg
     1021 tcctctcggt cgtgaccgcc ttcggcctcg ccgtgacccc gatcgccggc agtctggtcg
     1081 accgtgtcgg atcgaagcag atgctggtcg cgagccagat cacctgcgcg atcggctacg
     1141 ccggcctgct cctgatcccc gactcagtgc cgctcctgtt gctgaccgcc gggatgatta
     1201 ccgtcggtga atgcatcttc tgggtcggat atccgaacct cgtctcccag atcgccgatc
     1261 cggagcaccg ggatcgctgg ttcgccttca tgggcatgtc ccgcacggcc ggcttcggcc
     1321 tgggcgggtt gattgcctcg ggcgtgatcg cgattgtcgg cacgcacggc tacaaggtcc
     1381 tgctcggcgc gaacgtctgc accttcgcga tcgcggccac gatcatcctg atccgcgtgc
     1441 cccgggtccg cctatcgatg tcgacgcgcg agcacggcgg ctggatggcc gtgatccggg
     1501 accggcgcat catgttcctg gcggcagcac acggctttgg ggtgctaacg atcctgatcg
     1561 tcttccaggg gctgccgctc tacgtggtcg acgagctggg cctgcccggc tgggtccccg
     1621 gtctgctgct cggcgtgaac acggccatcc tcgccgcggg gcaatcgatc gggctccgga
     1681 tcgtgtccgg ctggcgacga accaggattt acgtactctg cgcggcgatc tggctggtcg
     1741 gcgcggggtt gtttgcgctc ggcgaattca tcccggccgg gatcgtcatc ccgtacctgt
     1801 tcctggcgat gatcattgcc agtggcggcg aggtgttcca tttgccccaa aacggcggcg
     1861 caccgaccgc ccttgccccg gaagcgctgc gcggccgcta cctggccctc ttctcgctgg
     1921 tctggagcgt cgccggcatt gcctcaccga cgctggtctc ggttctcctc tcgatcaacg
     1981 gcatcctgct ctgggtgggc atggccggcg cagccgtgat cactgccctg atcgccctcg
     2041 ccagcgagcc aatcatcgac cctaacgtcc agcggatgcc cgtgtgacga aggcgcgaag
     2101 gcgaaggcgc gaagattgta gggatctgcg ggggcaaatc cgttgcagcc agatgcccgg
     2161 ccgtgcatgc gagcccgttt tcccgcccgt ccgtttcaac cgacatccgg gccctggctc
     2221 tggatttcaa tccggagcgg cgccggac
//
LOCUS       DASSGX010000483         3362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_629455, whole genome shotgun sequence.
ACCESSION   DASSGX010000483 DASSGX010000000
VERSION     DASSGX010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3362
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629455"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            408..947
                     /locus_tag="VFI12_08670"
     CDS             408..947
                     /locus_tag="VFI12_08670"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET7056522.1"
                     /translation="METGGQREYSISELAEAAGVSTRTVRYYVSEGLLPPPVGSGPNS
                     RYTDVHRRQLGIIGRLKAQYLPLREIRRRLAGHGDVHGVVDSAASPGYHGMSRRDREI
                     AEDHLRREAIPTPPPSAPMAEPLFSAAYPPPEPDRDRPWRRVAISDEAELLIIEDQYR
                     RHQDKIDWLVQWAKRVLGS"
     gene            1056..2321
                     /locus_tag="VFI12_08675"
     CDS             1056..2321
                     /locus_tag="VFI12_08675"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET7056523.1"
                     /translation="MANHAPDPLVSVTSRWDRPAVATGPGETWLVVTVATAIPESDTP
                     RPPVDVAFVIDRSGSMSGLPLQLAKEGVLTALDQLNDEDGFSVVAYDDVPLTISRPAR
                     ATGKQRTMLAMTLAGLEPGGSTNLFGGWDLGCQHLMHPEFRVNGRVRRAILLTDGLAN
                     VGLIDSARIAQEVSARRAVGISTSTLGLGTGIDEAKLSAMAEAGGGSFAWVEHARDLP
                     GFFARELNEAISTVASGATITLTLPAGVRGRLLNPFPGERIGKRITVSLGELPAGITI
                     PLVFSITTRATDEGRFPPLALAAKWQEVNAAAAELIDVPVDPLMAVRPADFDSMPRDE
                     QAATAAARMIADDAQRQAMRHYRSGDVSMARQALGSAARFAASAPAGAGIASELDEMM
                     AYDPSSPEFQLLRRKIESDAHRRSRGRDV"
     gene            2443..2943
                     /locus_tag="VFI12_08680"
     CDS             2443..2943
                     /locus_tag="VFI12_08680"
                     /inference="COORDINATES: protein motif:HMM:NF024135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HET7056524.1"
                     /translation="MNVLVTAASKHGSTHEIGEAIATQIQSLGIPAEYCDLSAPMRHI
                     EASAVVLGSAIYMGKWLDDACDFVDKHRAQLQALPIWLFSSGPLGDEHPLPSDPPEDI
                     DDLCRDLNARDHRIFGGKLNPDDLGPGETALTELIDAPAGDYREWPDIHAWAYGIGEA
                     LLARGQ"
BASE COUNT          543 a         1254 c         1037 g          528 t
ORIGIN      
        1 tcacccgatg tcccgttgat cgtgcccgag ccgctcttgg tgacgatccg cccctgacag
       61 taggagaccg ccgaaaccgg ctcgccgaac ccccccgccc cgattgcgag cagcacaaga
      121 cagcagacga cacgagcgac cagcgtgcga cccattccca ggaacctccg gaacgcggcg
      181 accaaacagt aatagaaccg gttgaaaact cggccagatt atcaaaggga ataaacaaac
      241 gccatatccg gaggtcccga ccttggtctt cggtttccgg ttttcggttt tcggttttcg
      301 ggaacgcgga tagcgctccc tccatccgac tcctcgcctg ctcgcctgct cgcctcctcg
      361 cctgctcgcc tattgacagc agtacgtctt actgtcatga tgcatccatg gagaccgggg
      421 gacagcgcga gtacagcatc agcgagttgg cggaagcggc gggggtctcc acccgcacgg
      481 tccgctacta cgtctcggaa ggcctgctgc caccgcccgt cggcagcggc ccgaatagcc
      541 ggtacacgga cgtccaccgc cggcagctcg ggatcatcgg ccgtctgaag gcgcagtacc
      601 tgccgctccg cgagatccgg cgccggctgg ccggccacgg cgacgtgcac ggcgtcgtgg
      661 actccgccgc cagcccggga tatcacggga tgagccggcg ggatcgagag atcgccgaag
      721 atcacctgcg ccgggaggcg atccccacgc cacctccgag cgcgccgatg gcggagccgc
      781 tgttctccgc cgcatacccg cccccggagc cggatcgcga ccggccctgg cgacgggtcg
      841 ccatctccga cgaggccgag ctgctgatca tcgaggatca gtaccgccgc caccaggaca
      901 agatcgactg gctcgtccag tgggcgaagc gggtgctcgg ctcctgatcg cccgcccgta
      961 ctgactaacg aatcgaccca acccacgatt gcgccgacac ccgtccgcgc ccaggctcat
     1021 cgttgcccga attccgggca ggaaggaccg ccgacatggc gaatcacgca cccgaccccc
     1081 tcgtttctgt cacgtcccgc tgggatcgcc ccgccgttgc gaccggcccg ggcgagacct
     1141 ggctcgtcgt caccgtcgcg accgccatcc cggagagcga taccccgcgc ccgccggtcg
     1201 atgtcgcctt cgtcatcgat cgctccggct cgatgtccgg tctgcccctc cagctcgcga
     1261 aagagggcgt gctgaccgcg ctcgaccaac tcaacgacga ggatggcttc tccgttgttg
     1321 cgtacgacga cgtaccattg acgatcagcc ggccggcccg tgccaccggg aagcagcgga
     1381 cgatgctggc catgacgctt gccggcctcg aaccgggcgg ctcgaccaac ctcttcggcg
     1441 gctgggacct cggctgccag cacctgatgc accccgaatt ccgcgtcaac ggccgcgtcc
     1501 gccgcgcgat cctgctcacc gatggcctgg ccaatgtcgg gctgatcgac tcggcccgga
     1561 tcgcgcagga ggtgtcggcc cgccgcgcgg tgggcatctc gaccagcacc ctcggcctcg
     1621 gcaccgggat cgacgaggcc aagctctccg ccatggccga agccggcggg ggcagcttcg
     1681 cctgggtcga gcacgcgcgg gatctgcccg gcttcttcgc gcgggagctg aatgaggcca
     1741 tctcgactgt cgcctcgggc gcgaccatca cgttgaccct gccggcgggc gtccgtggcc
     1801 ggctgctcaa ccccttcccg ggcgagcgga tcggcaagcg gatcacggtc tcgctcggcg
     1861 agctgccggc cgggatcacg atccccctcg tcttctcgat cacgacccgg gcgaccgacg
     1921 agggccgctt cccgccgctg gcgctggcgg cgaagtggca ggaggtcaac gccgcggccg
     1981 ccgagctgat cgatgtcccg gtcgatccgc tgatggcggt cagaccggcc gacttcgact
     2041 cgatgccgcg ggatgagcaa gccgccacgg ccgcggcccg gatgatcgcc gacgacgccc
     2101 agcggcaggc catgcgccat taccgcagcg gcgatgtctc catggcccgc caggcactgg
     2161 gctctgccgc ccggttcgcc gcctccgcgc ccgccggcgc cggcatcgca tctgagctgg
     2221 acgagatgat ggcctacgac ccgtccagcc ccgagttcca gctcctgcgc cgcaagatcg
     2281 agagcgatgc ccatcgccgc tcccggggcc gcgacgtctg acccgcaccc tctcccatca
     2341 tcgtagcgcc ggccgccccc gaccggcgct cccccattgt cttcgtggca agatggggca
     2401 ggacatcacg gcccgagacg cacccccagc ggaggaagcg cgatgaatgt gctggttacc
     2461 gccgccagca agcacggcag cacccacgag atcggcgaag cgatcgcgac gcaaatccag
     2521 tccctcggca ttccggccga gtactgcgat ctgagcgccc cgatgcggca tatcgaggca
     2581 tcggccgtcg ttctcggcag cgccatctac atgggcaagt ggctcgatga cgcctgcgac
     2641 ttcgtcgaca agcaccgcgc ccaattgcag gcgctgccga tctggctctt cagcagcggc
     2701 cccctcggtg acgagcaccc gctcccgtcc gacccgcccg aggatatcga cgacctctgt
     2761 cgcgacctca acgcgcgcga tcaccgtatc ttcggcggca agctcaaccc ggacgatctc
     2821 ggccccggcg aaacggccct caccgaactg atcgacgccc ccgcaggcga ctaccgcgaa
     2881 tggcccgaca tccacgcctg ggcctatggc atcggtgagg cgctgctggc caggggacag
     2941 tgatgggtcg ttgggctcgg gacttcggac gtcagacttc ggacatcaga cattggtttt
     3001 ccgttttccg ttttcggttt tccgagccgt tccggggttg aaaccccggg ccgagaacac
     3061 gacgcctctc cgaggctgac gacgaaagga ttggaatggg cgcacggccg atcgacagtt
     3121 ccggttccag tttcgcaccc gctcgtctgc ccgtcccgcc cggggttgaa accccgggct
     3181 caggcaacga agcctctccg aggctgaacg cggaatgatt gggatcggcg acgagcggat
     3241 ctgcccccgc agatccctac aatctccgcg cctccgcgcc tccgcgtctt cgcgtcttcg
     3301 cgccttcgcg ccttcgcgcc ttcgcgcctc cgcgcctctg cgcctccgcg ccctctcttc
     3361 tc
//
LOCUS       DASSGX010000484         2472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1174986, whole genome shotgun sequence.
ACCESSION   DASSGX010000484 DASSGX010000000
VERSION     DASSGX010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174986"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..138)
                     /locus_tag="VFI12_08685"
     CDS             complement(<1..138)
                     /locus_tag="VFI12_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET7056525.1"
                     /translation="MGKAKFERTKPHLNVGTIGHVDHGKTSLTAAITKTLSLKGEAAF
                     RS"
     gene            complement(201..2309)
                     /gene="fusA"
                     /locus_tag="VFI12_08690"
     CDS             complement(201..2309)
                     /gene="fusA"
                     /locus_tag="VFI12_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET7056526.1"
                     /translation="MTATATKMSAAQPTAQLERTRNIGIIAHIDAGKTTTTERILFYT
                     GRVHRPGETHEGAATMDWMEQERERGITITSAATTCSWKDYRINIIDTPGHVDFTVEV
                     ERSLRVLDGGVVVFDAVAGVEPQSETVWRQADKYHVPRFCFVNKMDRTGASYERTLEM
                     IVDRLKATPVPVQWPIGAESDFKGVIDLIDERAYIYHDDMGKNIEEIDVPDNYKEKVA
                     ETRRHIIEIIAESDEELMMRYLEDDVLTPDELRSALRAATLKGDLVPVLCGSSLKNKG
                     IQLMLDAILYYLPSPMDIPPVVGTDPRTDEEVTRAADPGAPFSGLVFKIQADPHIGKL
                     AYCRVYSGSLDSGSYVLNSSKSNRERVGRLVRMHANHREEVQSVSAGDIVAIIGLKSS
                     FTGDTLCDPDSPVLLESITFPEPVISVSIEPKTRADQDKMGTALARLAEEDPTFRVRT
                     NEETGQTLIDGMGELHLEVIVDRMMREFRVDATVGRPQVAYKETITLPVRAEGRHVRQ
                     TGGAGQYGHCVVEFSPQERGVGYQFEDKIVGGSIPREFIAPIDAGIRDAMAGGGASGY
                     PVVDIKAVLVDGSYHDVDSKEVAYKIAGSLALKEAIRRGKPAILEPIEKVEVTTPEEF
                     MGDVIGDLNARRGHVQGMEERAGAQVIRAFVPLATMFGYATDLRSATQGRAVYSMEFD
                     HYAPVPANIAEEMMAKARRD"
     gene            complement(2365..>2472)
                     /locus_tag="VFI12_08695"
     CDS             complement(2365..>2472)
                     /locus_tag="VFI12_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406077.1"
                     /note="binds directly to 16S rRNA where it nucleates
                     assembly of the head domain of the 30S subunit; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HET7056527.1"
                     /translation="GELLDAANGTGATIKRREDTHRMAEANRAFAHYRY"
BASE COUNT          416 a          798 c          777 g          481 t
ORIGIN      
        1 gctgcgaaac gccgcctccc ccttcaacga caacgtcttc gtgatcgccg ccgtcaaact
       61 cgtcttgcca tgatccacat gaccaatcgt gcctacgttc aaatgcggct tcgtccgctc
      121 aaatttggcc ttgcccattg cgtcctggtt cctctctata acgtgcgggc ctcatgccca
      181 cttagtcctg actgaaacga ctagtcgcgg cgagccttgg ccatcatctc ttcagcgatg
      241 ttggccggca ccggcgcgta atgatcgaac tccatcgagt agaccgcccg gccctgcgtg
      301 gccgaccgca ggtcggtcgc ataaccgaac atcgttgcca gcggcacgaa cgcccggatc
      361 acctgcgccc cggcacgctc ttccatgccc tggacatggc cgcgacgagc attcaggtcg
      421 ccgatgacgt cacccatgaa ctcttcgggc gtcgtgactt cgaccttctc gatcggctcg
      481 aggatggccg gtttgccccg gcggatggct tccttgagcg ccagggaacc ggcgatcttg
      541 taggcaactt ccttcgagtc gacgtcgtgg tacgagccgt cgacgaggac cgccttgatg
      601 tcgacgaccg gatacccgga cgcaccgcca ccagccatcg catcgcggat gccggcatcg
      661 atcggagcaa tgaactcgcg cgggatcgag ccgccgacga tcttgtcctc gaactgatag
      721 ccgacgccac gctcttgcgg cgagaactcg acgacgcagt ggccgtactg gccggcgccg
      781 ccggtctggc ggacgtgccg cccctcggcg cgaaccggga gcgtgatcgt ctccttgtaa
      841 gcgacctgcg gccggccgac agtcgcatcg acgcggaact cgcgcatcat gcgatcgacg
      901 atgacttcga gatgaagctc gcccatgccg tcgatcaggg tctggccggt ttcctcgttg
      961 gtccgcaccc ggaacgtcgg gtcctcctcg gcgaggcggg ccagcgctgt gcccatcttg
     1021 tcctggtcgg cgcgggtctt cggctcgatg gagaccgaga tgactggctc ggggaacgtg
     1081 atggactcca gcagaaccgg gctgtccgga tcacagagtg tgtcgccggt gaacgacgac
     1141 ttgaggccga tgatagcgac gatgtcgccc gccgagaccg actgcacctc ttcgcggtga
     1201 ttcgcgtgca tgcgaacgag gcgaccgacg cgctcgcggt tgctcttgct cgagttaagc
     1261 acgtaactgc ccgaatcgag cgagccggag tagacgcggc agtaggccag cttgccaatg
     1321 tgcggatctg cctgaatctt gaacaccagg cccgagaacg gcgcgcccgg atcggccgcg
     1381 cgcgtgacct cttcgtcggt gcgcggatcg gtacccacaa ccggcgggat gtccatcggt
     1441 gacggcagat agtacaggat ggcgtccagc atgagctgga tgcccttgtt cttgagcgac
     1501 gagccgcaga gaaccgggac cagatcgccc ttgagggtgg ccgcgcgcag agccgagcgc
     1561 agctcgtccg gcgtcaacac gtcgtcctcg aggtagcgca tcatcagctc ttcgtcggat
     1621 tcggcgatga tctcgatgat gtgccgacga gtctcagcca ccttctcctt gtagttgtcg
     1681 ggaacatcga tctcttcgat gttcttgccc atgtcgtcgt ggtagatgta cgcgcgctcg
     1741 tcgatcaggt cgatgacgcc cttgaaatca gactccgcgc cgatcggcca ctggaccggg
     1801 accggcgtag ccttcaggcg atcgacgatc atctcgaggg tgcgctcata cgacgcgccg
     1861 gtgcggtcca tcttgttgac gaagcagaac cgcgggacgt ggtacttgtc ggcctgccgc
     1921 cagacagtct ctgactgcgg ctcgacaccg gcgacggcgt cgaacacgac aacgccaccg
     1981 tcgagcacgc gcagcgagcg ctccacctcg accgtgaagt cgacgtggcc gggcgtgtcg
     2041 atgatattga tccggtagtc cttccagctg caggttgtcg cagccgaagt gatggtgatg
     2101 ccgcgctcgc gctcctgctc catccagtcc atcgtggcgg cgccttcatg cgtctcgccc
     2161 ggacggtgaa cgcgtcccgt gtagaagagg atgcgctcgg tggtggtcgt cttgccggcg
     2221 tcgatgtggg caatgatgcc gatattgcgg gtccgctcaa gttgagcggt cggctgggcg
     2281 gcggacatct tggttgccgt agctgtcatg cgttcgttcg tcctctaaat aatccgcgac
     2341 ggacccagtc cgccgcatgt ccgcctagta gcggtaatgc gcgaaggcgc ggttggcctc
     2401 ggccatgcgg tgcgtatcct cgcgccgctt gatcgtggcg cccgtgccgt tggcggcatc
     2461 aagcaattcg cc
//
LOCUS       DASSGX010000485         3810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_193720, whole genome shotgun sequence.
ACCESSION   DASSGX010000485 DASSGX010000000
VERSION     DASSGX010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193720"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            314..>3810
                     /locus_tag="VFI12_08700"
     CDS             314..>3810
                     /locus_tag="VFI12_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056528.1"
                     /translation="MGSTRSHGRTNPRAGNRRLLAAAAIVVLVVESLGSLGHSVAAAD
                     NQVNDIADGEHASPDGRVVISKPAPITTPGGAGGLTEDMSIGGPPLFLPYTPPAGVTE
                     LDGERTRQSRTLANPDGTFKTEISDGPINFQDAKGAWQPIDLSLVGDAINGYKVAATA
                     GDMRLGDINGELGSIELNGHKVSISPTEYLVGQKGEGLDTNKLDFAALSEGAPSLWIR
                     PVDIGLEFGATWANPSLTPSVTLLLDAGDLTPAIAKDGRSIEFTDAKGEFAGGIDAPV
                     LREGSEDGPPNMEQVSVSLTQAGPEYALTYVVDGKWFEAPERTFPIILDPTVCIGNGV
                     SGCTSGSFDTFVFDDDPDSYELNWTVLRTGYDSRTDEGTYGKMRSILYFPQVTLPDGA
                     VIYDTDLQAKVCCLYGSAQGETVSAYRLTKLLTSHYTWNKFGNAFDLNGGVSDTVPAS
                     GYMNWDVDDIVTSFYTRRAKLYKPPYGFLLKMATESSSHGEVEFRRYNYGTTADRPLL
                     TINYTLPVVGIDFDPRLGDTYAPSSMVAGQTVKLPIVVQDKTGSADVMSKCVLNDTDC
                     WKVGYRWFDGKGNLAGGGDGAATVDLPEDVAVNDSSNSPFVLNVTPPSTTGQYTLTLE
                     LVHYRTGHYWWSSDFASPSLYYSRNKKILTSSSTRWTGSSAIERDEFSINVTEGSAGS
                     NTQVVPVSSGGTVGIDLGTKNLEVQADSGLGFSDREPLRLTYGYDEALAELCSTYQGI
                     LGACGWHTNFDERVTGGSNQTGYDYMYFGPDGTPAMLDTDDSGQIAGGAPVLFNRQRV
                     TILDENGGWDSSDSGTLPDIGAITPVGFTPWSGSLIAQTTSTADVLVNGPDKVSLNVY
                     RKVRFAMRTSAASKAGMCFQIHNVSDAANFPDRWYCYTLGDTSWSAGSEYQYFPTSTL
                     TTAWKYIDDDLYNRVRDAGDYGSTSDDYQVTAIRIEGAPGATGTTYVDAFRFESDENP
                     FIDDSPPTWTTGSTSTSTDAIVGSYSLEITPGSGITPTCRTSNSCWLTTQGGTWAYPF
                     WDWYWKKQGGTAAAMVFYIHDERSGAHCNTADCTLTYYAGAAPKIVGGGQYDAVVRID
                     SNLPRTWTQVRRNVAADAAQALNLFDDAGTGAADLIRVTGYAPVAVDGSSLLLDRMAY
                     GNLADIGSVDRTNQGGALAG"
BASE COUNT          729 a         1262 c         1169 g          650 t
ORIGIN      
        1 cagcgcagcg atgggcgcaa gacgacgctc cacgatcgac ctccgacagg gtgtcagcga
       61 ggccgggcgg gacggctccg gggttgtagc agcggcggct cacctgccga tcacctgcgg
      121 cttatcggcg gatcacccaa acgcagtcgc gcaattcgcg gcgaaactcg ccgcaaccat
      181 cgcgccgcgt tgcgggaatc gagcttcgcc tagccgtgtt cggatttcat agggttttcc
      241 ctacttccca acatcagcat tggggtctag cgtccgccga aggttgtgga cactggctcg
      301 gggagggtcg cgggtgggtt cgacgcggtc gcacgggcgg acgaacccgc gcgcaggtaa
      361 tcggcggcta ttggccgccg ccgccattgt cgtcctggtg gttgaaagcc tcggatcgct
      421 aggccattcg gtggccgccg cggacaacca agtcaacgac attgccgatg gcgagcacgc
      481 ttctcccgac ggacgcgtcg tcatatcaaa acccgcgccg atcacgacgc ccggtggcgc
      541 cggtggccta actgaagaca tgtcgatcgg cggcccgccg ctcttcctcc cgtacacccc
      601 tcccgccggc gtaacggagc tcgatggtga gcgcacccga cagagtcgga cgctcgcaaa
      661 ccccgatggc acgttcaaga ccgagatcag cgatggaccc atcaactttc aggacgcgaa
      721 gggcgcctgg cagccgatcg acctgtcact cgttggcgac gcgatcaacg gctacaaggt
      781 cgcggcgact gccggcgaca tgcggctggg cgacatcaat ggcgagctcg gctccatcga
      841 gctcaatggt cacaaggtca gtatctctcc gacggagtac ctcgtcggcc agaaaggcga
      901 aggcctcgac accaacaagc tcgacttcgc ggcgctgtcc gaaggcgctc cctcgctgtg
      961 gatccgtccc gtcgacatcg gcctcgagtt tggggcgacg tgggcaaatc cgagcctgac
     1021 gccgtcggtg accttgctcc tggatgcagg cgacctgacg ccggcaatcg ccaaggatgg
     1081 tcgcagcatc gagttcaccg acgcgaaagg cgagttcgcc ggcgggatcg atgcgccagt
     1141 cctgcgcgaa ggttctgagg acggcccgcc gaacatggag caggtctcag tcagcctgac
     1201 gcaagccggg ccggagtacg ccctgaccta cgtcgtcgac ggcaaatggt tcgaggcgcc
     1261 cgagcgaacg ttcccgatca tcctcgatcc cacggtctgc atcggcaacg gcgtctcggg
     1321 ttgcacttct ggaagcttcg acaccttcgt gttcgatgac gatccggaca gctatgagct
     1381 gaactggacc gtcctgcgga ctggctatga cagccgaacg gacgaaggca cgtacgggaa
     1441 gatgcgctcc atcctgtact tcccgcaggt cacgctgccg gacggggccg ttatctatga
     1501 caccgatctt caggccaagg tctgctgcct ctacggcagc gcccagggcg agaccgtctc
     1561 cgcgtatcgg ctcacgaagc tcctgacgag ccactacacg tggaacaagt tcggaaacgc
     1621 cttcgacctc aatggcggcg tatcggacac agtgcccgcc tcgggctaca tgaactggga
     1681 tgtcgacgac atcgttacga gcttctacac gcgccgcgcc aagctataca agccgccgta
     1741 cggcttcctg ctcaagatgg ccactgaaag cagcagccat ggcgaggtcg agttccgccg
     1801 ctacaactac ggcaccaccg cagaccgccc gctgctgaca atcaactaca cgctgcccgt
     1861 cgttgggatc gattttgatc ccaggctcgg cgatacctat gcgccatcga gcatggtcgc
     1921 cggccagact gtcaagctgc cgatcgtcgt ccaggacaag acgggctcgg ccgatgtcat
     1981 gagtaaatgc gtcctcaacg acaccgattg ctggaaggtc ggctaccgct ggtttgatgg
     2041 gaagggcaac ctcgccggcg gcggggacgg cgcggcgacc gttgaccttc ccgaagacgt
     2101 cgcggtcaac gactcgagca actcgccctt cgtcctcaac gtcaccccgc cgtccacgac
     2161 cgggcagtac accctcacgc tcgagctcgt tcactatcgg actggccatt actggtggtc
     2221 gagcgacttc gcgagcccgt cgctctacta cagccgcaac aagaagatcc tgacatcgag
     2281 ctcgacgcgt tggaccggtt cgtctgcgat cgagcgcgat gagttcagca tcaacgtcac
     2341 tgaaggctcg gcgggctcga atacgcaagt agtgccggtg tcgtccggtg ggaccgttgg
     2401 catcgacctc ggcacgaaga acctcgaggt gcaggctgac tcagggctcg gcttcagcga
     2461 ccgcgagccg ctgcgactga cgtacggata cgacgaagcg ctcgccgaac tttgctcaac
     2521 gtaccagggc atcctcggcg cctgcggctg gcatacgaac ttcgatgagc gcgttaccgg
     2581 tggctcgaac cagaccgggt acgactacat gtactttggt ccggacggca cgcccgccat
     2641 gctggacacc gatgattccg ggcagatcgc cggtggggcg ccggtcctgt tcaatcggca
     2701 gcgcgtcacg atcctcgacg agaacggcgg ctgggacagc tcagattccg gaaccttgcc
     2761 cgacatcggc gcgatcacgc cggtgggctt caccccgtgg tccgggtcgc tgatcgccca
     2821 gacaacctcc accgccgatg tgcttgtcaa cggtcccgac aaggtctcgc tcaacgtgta
     2881 tcgaaaggtc cgcttcgcga tgcggacgtc cgcagcctcc aaggccggaa tgtgcttcca
     2941 aatccacaac gtcagcgacg ctgccaactt cccggaccga tggtattgct acaccctcgg
     3001 ggatacgtcg tggtccgcag gcagcgaata ccagtatttc ccgaccagca cgctgacgac
     3061 cgcgtggaag tacatcgacg acgatctgta caaccgcgtc cgcgacgccg gcgactacgg
     3121 ctcgacgagc gatgactatc aggtaactgc gatccgtatc gaaggggcgc cgggcgcgac
     3181 cggtacaacg tacgtcgacg ccttccggtt cgagagtgac gagaacccgt tcatcgacga
     3241 ttctccgcca acctggacga ccggctcgac ctcgacgtcg accgacgcga tcgtgggctc
     3301 gtactccctg gagatcacgc ccggctcggg gatcaccccc acatgccgaa cgtcgaattc
     3361 ctgctggctt actactcaag gtgggacgtg ggcctatccg ttctgggact ggtactggaa
     3421 gaagcagggc ggcacggccg ccgcaatggt cttctacatc cacgatgagc ggagcggcgc
     3481 acactgcaac accgccgatt gcaccctgac ctactacgcc ggcgccgccc ccaagatcgt
     3541 gggtggcggc caatacgacg cggtcgtacg catcgattcg aaccttccac ggacgtggac
     3601 ccaggttcgg cgcaatgttg cggcggacgc ggcgcaagcc ctgaacctgt tcgacgacgc
     3661 cggtactggc gccgccgacc tgatccgcgt cactggctac gccccggtcg ccgtcgacgg
     3721 atcctcgctg ctgctcgacc gcatggcata cggaaatctc gccgacatcg ggtccgtgga
     3781 tcgaacgaac cagggcggcg cgcttgccgg
//
LOCUS       DASSGX010000486         3077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_739093, whole genome shotgun sequence.
ACCESSION   DASSGX010000486 DASSGX010000000
VERSION     DASSGX010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739093"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VFI12_08705"
     CDS             <1..190
                     /locus_tag="VFI12_08705"
                     /inference="COORDINATES: protein motif:HMM:NF024682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056529.1"
                     /translation="AQSLRIFPPLWQERIEAVPPDNLDVIKRLIADHIASMTEQQVCD
                     TFEKLTGRGQASAVEPIA"
     gene            complement(194..2566)
                     /locus_tag="VFI12_08710"
     CDS             complement(194..2566)
                     /locus_tag="VFI12_08710"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HET7056530.1"
                     /translation="MSRYYSPYATRNKPRRWPAVLAVFLALVIGAGVGIAATGGLNIF
                     SSGASDPTETPRAIAANDQTSTAEPTEVEPASTATDLSPGEEQAVTNTAVPDQADQTE
                     TPQPTATTTPADTPTPDAGPPDEMAKRFAETWSKGDYGALYKMISVNSKAAISEQDFV
                     DRFEAINAEAGITEVKVEITGAPDLELNQPIKVSYKTGTVGDFAQTNTLHLVREGAEW
                     HVDWSPSLIFTQLGDGCVDFMVQTVRRGSILDRNGDPLAYDGSASVVGVIPAQFLNED
                     KELKDLSKIVGIDAKDIKSMYAEADPGWFVPIKTFPNQVDDATKTKLAAIPGAALRTQ
                     TARIYPLGKQAAHITGYVTRVTAEDIEKDPTGELAGIDWIGRAGIEAGANDLLTGRPG
                     GRLMVVDCSSRAERTEIASRRAQEPKDVVLTIDKNFQIQVDNALGDVKGSAVVIDPRT
                     GGIMALASHPAYDPNWFVSGMTGKDRDFINNEDERPLLDRAAEAAYPTGSIFKVITMA
                     AAMHDLKYTGDTQIDCPQEFSLPGTDQVWRDWTYEEGLGAQGMLTLHTGLVNSCNTVF
                     YQLGAALDEQNEEWLPDMAKAFGLGAPTGIPYFPEVSGTVPSPEWKQQVMGDYWARGD
                     AVNMAIGQGFVEATPLQMANAYTAIANGGTLLQPFIVEFQQNPDGSQKRIGKRTKIDD
                     LPLSDDQVAEIQSALRDQTSNSWGAGSVRVFGDFAWPIAGKTGTAQNQMTVEQTPHSW
                     FAAFGPYGEEATIASIVMVESSGEGVTFAAPRTRQIYEAYLKTDLMNKKT"
     gene            2802..>3077
                     /locus_tag="VFI12_08715"
     CDS             2802..>3077
                     /locus_tag="VFI12_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET7056531.1"
                     /translation="MTSPQTDHRPLAGIRVVDLTRVMTGPYCTMMLGDLGADVIKIEL
                     PGKGDDTRAWGPPFIHGEAAYYLSVNRNKRSIALDLKREAGKEALWKL"
BASE COUNT          471 a          995 c         1011 g          600 t
ORIGIN      
        1 tgcgcaatcg cttcggatct tcccgccgct ctggcaggag cgcattgagg cggtgccgcc
       61 ggacaatctc gatgtcatca agcgactgat tgccgaccac atcgcctcga tgactgagca
      121 gcaggtctgt gacaccttcg agaaactgac cggtcgcggc caagcctccg ccgtcgagcc
      181 gattgcgtag gcgtcacgtt ttcttgttca tcaggtcggt tttgaggtag gcctcgtaga
      241 tttggcgggt gcgcggcgct gcgaaggtga cgccctcgcc gctgctctcg accatcacga
      301 tcgaggcaat cgtcgcttcc tcgccgtatg gcccaaacgc cgcgaaccac gagtgcggcg
      361 tctgctcgac cgtcatctgg ttctgggcgg tgcccgtctt gcccgcgatc ggccacgcga
      421 aatcgccgaa gacgcgaacc gagcccgcgc cccacgaatt gctggtctga tcccgcagcg
      481 cgctctggat ctcggcgacc tgatcgtcgc tgagcggcag gtcgtcgatc tttgtccgct
      541 tgccgatccg tttctggcta ccgtccgggt tttgctggaa ctcgacgatg aagggctgga
      601 gcaatgtgcc cccgttcgcg atcgcggtgt aggcgttcgc catctggagc ggcgtcgcct
      661 cgacgaagcc ttgcccgatg gccatattga cggcgtcgcc ccgcgcccag taatcgccca
      721 tgacctgctg cttccactcg gggctcggta cggtgccgct cacctcggga aagtagggta
      781 tgccggtcgg cgcgccgagg ccgaaggcct tggccatgtc cggcagccac tcttcgttct
      841 gctcatcgag cgcggcgccg agctggtaga agacggtgtt gcaggagttg accaggccgg
      901 tgtgcaacgt cagcatgccc tgtgcgccaa gcccttcctc atacgtccag tcccgccaga
      961 cctgatcggt gccaggcagg ctgaactcct gcgggcaatc gatctgcgtg tcgccggtgt
     1021 acttgaggtc gtgcatggcg gcggccatgg tgatgacctt gaagatcgag ccggtcgggt
     1081 aggccgcttc ggccgcgcga tcgagcaatg gccgctcatc ctcattgttg atgaagtcgc
     1141 ggtccttgcc ggtcatcccg ctcacaaacc agttcgggtc gtaggccggg tggctggcca
     1201 gcgccatgat cccgccggtg cgcggatcga tcacgacggc gctgcccttg acatcgccga
     1261 gcgcgttgtc gacctggatc tggaagttct tgtcaatcgt caggacgacg tctttgggct
     1321 cctgcgcccg gcgggaggcg atctcggtcc gctcagcccg cgagctgcaa tcgacgacca
     1381 tcaaccggcc gcccggccgc ccggtcaaca ggtcattcgc gcccgcctcg atccccgccc
     1441 ggccgatcca gtcgatcccg gccagctcgc cggtcgggtc tttctcgatg tcctcggccg
     1501 tgacccgggt gacatacccg gtgatgtgcg ccgcctgctt gccaagcggg tagatgcgcg
     1561 ccgtctgcgt gcgaagtgcc gcgccgggga tggcggccag cttggtcttg gtcgcgtcgt
     1621 cgacctggtt cgggaaggtc ttgatcggca cgaaccagcc cggatcagcc tcggcgtaca
     1681 tcgacttgat gtccttggcg tcgatcccga cgatcttgct caggtccttg agctccttat
     1741 cctcgttcag gaactgcgcc gggatgacgc cgaccacgct ggccgagccg tcatatgcca
     1801 gcgggtcgcc gttgcggtcg aggatgctgc cacgccggac ggtctggacc atgaaatcga
     1861 cgcagccatc gccgagctgc gtgaagatca gcgagggcga ccagtcgaca tgccactcgg
     1921 cgccctcgcg gacgaggtgg agcgtgttgg tctgcgcgaa atcgccgacg gtgccggtct
     1981 tgtaactgac tttgatgggc tggttgagct cgaggtccgg cgcaccggtg atctcgacct
     2041 tgacctcggt gatgccggct tcggcattga tcgcctcgaa ccggtcgacg aagtcctgtt
     2101 cgctgattgc cgccttcgaa ttgacgctga tcattttgta gagcgcgccg taatcaccct
     2161 tgctccaggt ttcggcgaag cgcttggcca tctcatcggg ggggccggca tcgggcgtcg
     2221 gcgtgtccgc gggcgtggtg gtcgcggtcg gctgtggtgt ctcggtctgg tccgcctggt
     2281 cggggaccgc cgtattcgtc accgcctgct cctcgcccgg cgagaggtcg gtcgcagtcg
     2341 aggccggctc aacctccgtg ggctcggccg tgctggtctg gtcgttcgcg gcgatggcgc
     2401 gcggcgtctc ggtcggatca gatgcgccgg aggagaagat gttgagtccg ccggtcgcgg
     2461 caatgccgac acccgcgccg atcacgagcg cgaggaaaac ggcgaggacc gcgggccacc
     2521 gtcggggctt gtttctggtg gcgtagggag agtagtacct ggacaacggc ggatgaactc
     2581 cgatccaacc aacctggctg gccacacggg cgtgcgggga cgattgcgtg cggggcgccc
     2641 tcatgctgac aggggcagcc cgtttcgtca agctaaacgc gccctgctct gcataacgta
     2701 acggatgagg ccatgctcgg gtttcagcgg ccggtcggag cgtcctgctt gctacaatct
     2761 gacgtcgata ccgccaccgt ttgcatgcag aaggatcacc tgtgaccagc ccccaaaccg
     2821 atcatcgccc cctcgccggc atccgggtcg tcgacctgac ccgcgtcatg accggcccct
     2881 actgcacgat gatgcttggg gacctcggcg cggacgtgat caagatcgag ctgcccggga
     2941 agggggacga cacccgcgcc tggggaccgc cctttattca tggtgaggcg gcctactacc
     3001 tttccgtaaa ccgcaataag cgatcgatcg cgctcgacct caagcgcgag gccggcaaag
     3061 aggcgctctg gaagctg
//
LOCUS       DASSGX010000487         2974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_630165, whole genome shotgun sequence.
ACCESSION   DASSGX010000487 DASSGX010000000
VERSION     DASSGX010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_630165"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2974
                     /locus_tag="VFI12_08720"
     CDS             <1..>2974
                     /locus_tag="VFI12_08720"
                     /inference="COORDINATES: protein motif:HMM:NF021570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HET7056532.1"
                     /translation="IRATDDGGWWHLASGLAVALAAASKINALPIVAVLGLPVFERWR
                     TIGIRSVVRFRDGSRLSPLAGAAIALIAAIWTFRIAQPYAFKGPNPFSFALDPRWLND
                     LRYWREVQAGVGDSPPSMQWADRTPVLFSIRNLVLWGMGPLLGLTSLIALALAGLRVL
                     AARRLPPGWQLILVGWPAFHLLYYGGSFVQTMRYLLPAYPFLVLLAAALLIRIADLGT
                     ERGWGSIALGRRHALPRGAIPAAIVLIGTALYAVGFSGIYTRTTTREAASRWIYANVE
                     PGAVIASEHWDDAIPLPLPGFDSQQYPGVQLELFFPDDAAKLTTVLNRLSQADYVVVT
                     SNRLYGTIPRIPERYPMTAEYYRMLFSGDLGFDLVHTETSYPTYAGFELNDDAAEEAF
                     TVYDHPKVLVFRKSERFDLGAVTSRLTAALGNGGYAISPSVAGENLLMMDDATRAEQQ
                     ASGTWRRMFDPGSLPNRFPVIIWYLAVQIMALAAVPFCWRLFPALPDRGHAVAKTFGV
                     LGSAYGAWMLASLRVFAFGPDAVGFGIVLVGAVSAIAVRDRFRELAHDVRERWRSVVV
                     VEGTFLVCFLAAVSVRGRNPDLWERYRGGEKPMDFAYFNAVQRSVSFPAFDPWFAGGY
                     LHYYYFGFVQWASVTRLTGIVPEVAYNLAIPTIFALLSLNVWAVAVALIGATRPGLRA
                     REGWRLPTLGLLAPLFVAVLGNLDLAQRIGRGEFGYPAGPGSSRAGGIGDIVAGLWNL
                     VAHHPAHPPDIYWSATRIVPGTINEFPAFSLLFADLHAHVMAMPLASATLVLACQIAL
                     GSGEQSRQSTADDPDTPGLTRSATMFPSMSNANGGALRSFLSSSRATAGRFALEAGAA
                     GFMASAIYATNTWDFPTYLGLLVGAKAIRECLAVEWHVSYRLLVRIGLWAIATVAAGR
                     LFFWPFYASFFPESGVVRQPERTPVASYLTIHGLFLVALAGFVTLNLVQRLALSRSLI
                     RLPATAGIVAEPAPIATGT"
BASE COUNT          417 a         1041 c          939 g          577 t
ORIGIN      
        1 gattcgggcc accgacgacg gcggctggtg gcatctggcg agcggtctcg cagtcgctct
       61 ggcggcggcc tcgaagatca acgccttgcc gatcgtcgcg gtcctcggat tgccggtttt
      121 cgagcgatgg cgaacgatcg ggattcgatc ggtcgtgcgc ttccgggacg ggtcgcggct
      181 gtcgccactg gcgggtgccg ccattgcctt gatcgcggct atctggacgt tccgaatcgc
      241 ccagccctat gccttcaagg gcccgaaccc gttcagcttc gcgctcgatc ctcgctggtt
      301 gaatgacctc cgatactggc gcgaggtcca ggcaggcgtc ggggactctc caccgtccat
      361 gcagtgggcc gaccggactc ctgtcctgtt ctcgatccgg aacctcgtgc tgtggggaat
      421 gggtcccctg ctgggcctca cctcgctgat cgcgctcgcc ctggccggtc tgagggtgtt
      481 agccgcgcgt cgcctgccgc caggatggca gctgatcctg gtcggctggc ctgccttcca
      541 tctgctctat tacggcggca gcttcgtgca gacgatgcga tatctgctgc cggcctaccc
      601 gttcctggtg ctgctcgcgg cggcgctcct gatccggatc gccgatctcg gcacggaacg
      661 cggctggggt tcgatcgcgc tcggacgccg gcacgcattg ccccgcggcg cgatccccgc
      721 cgccatcgtt ctcatcggga cagccctgta cgcggtcggg ttcagcggca tctatacccg
      781 gacgacgacc cgggaagcgg cctcgcggtg gatctacgcc aacgtcgaac cgggcgctgt
      841 aatcgcctcc gagcattggg atgacgccat tccccttccc ctccccgggt tcgactccca
      901 gcaatatccg ggagttcagc tcgagctgtt cttcccggac gatgctgcca agttgacgac
      961 ggtcctcaac cggctctcgc aagccgacta cgtcgtggtc acgagcaacc ggctctacgg
     1021 cacgatcccg cgaatccccg agcgataccc gatgacggcc gagtactacc ggatgctctt
     1081 ctcgggcgat ctcggcttcg accttgtcca caccgagacg tcgtacccca cctacgccgg
     1141 attcgagctc aacgatgacg cagcggaaga ggcgttcacc gtctacgacc atccgaaggt
     1201 cctcgtattc aggaagagcg aacggttcga cctgggagcg gtcacatccc ggctgaccgc
     1261 cgccctgggg aacggcggat acgcaatttc gccgtcggtt gcgggcgaga acctgctgat
     1321 gatggacgac gccacccgcg ccgagcagca ggcgagcggg acctggcggc gcatgttcga
     1381 ccccggcagc ctgccgaacc gcttcccggt catcatctgg tacctcgcgg tgcagatcat
     1441 ggcgctggca gcggttccct tctgctggcg actcttcccg gcactgccgg accggggaca
     1501 cgcggtcgcg aagacgttcg gcgtgttggg gtcggcgtac ggcgcctgga tgctggccag
     1561 cctcagggtg ttcgcgttcg gcccagatgc cgtcgggttc ggcatcgtcc tggtcggagc
     1621 ggtgtcggcg attgccgtcc gcgaccggtt ccgggagctc gctcacgatg ttcgcgagcg
     1681 ctggcggagc gtagtcgttg tcgagggcac gttcctcgtc tgctttctcg ccgcagtctc
     1741 cgtgcgggga cgaaacccgg acctttggga gcgctaccgg ggcggcgaaa agccgatgga
     1801 cttcgcctac ttcaacgcgg tgcagcgcag cgtctccttc cccgccttcg acccatggtt
     1861 cgccggaggc tacctccatt actactactt cggcttcgtg cagtgggcat cggtcacccg
     1921 cctgacgggg atcgtgcccg aagtcgccta caacctcgcc atcccgacca tcttcgcgct
     1981 gctgagcctg aacgtctggg cggtcgcggt cgccctgatc ggcgcgaccc ggccgggcct
     2041 tcgcgcgcgc gagggctggc gactcccaac gctggggttg ctggcgccgc tgttcgtggc
     2101 cgtattgggc aacctcgacc tcgcgcaacg catcgggcgg ggcgagttcg gctacccggc
     2161 tggaccggga tcgtcccggg ccgggggcat cggcgacatt gtcgccggtc tctggaacct
     2221 ggtggcgcat catccagccc acccgccgga catctattgg tctgccaccc ggatcgttcc
     2281 cggcacaatc aacgagttcc cggccttcag cctcttgttc gccgatctcc atgcgcacgt
     2341 gatggcaatg ccgctcgcat ccgccacgct ggtgcttgcc tgccagatcg cgctcggctc
     2401 cggggaacag tcgcggcaat caaccgcaga cgaccccgac acacctggct tgactcgctc
     2461 ggccaccatg tttccgagca tgagcaacgc gaacggcggt gccctccgct catttctttc
     2521 cagctccagg gccacggcag gtcggttcgc gctcgaagcg ggcgctgccg gcttcatggc
     2581 cagcgccatc tacgccacca acacctggga cttcccgacc tacctcggcc tgctcgtcgg
     2641 cgcgaaggcg atccgggaat gcctggctgt tgaatggcac gtctcatatc gactgctggt
     2701 tcggattggc ctctgggcga tcgcgaccgt cgccgccggc cggctcttct tctggccctt
     2761 ctacgcgtcg ttctttccgg agtccggcgt tgtccggcaa cccgagcgaa ctccggtcgc
     2821 ctcgtacctg acgattcacg gcctgttcct ggttgcgctc gccggtttcg tcacgctgaa
     2881 tctggttcag cgcctcgcct tgtcccggag cctgatccga ctgccggcga cagccggcat
     2941 cgtcgctgaa ccggcgccca tcgccaccgg gaca
//
LOCUS       DASSGX010000488         2784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1065270, whole genome shotgun sequence.
ACCESSION   DASSGX010000488 DASSGX010000000
VERSION     DASSGX010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065270"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1142
                     /locus_tag="VFI12_08725"
     CDS             <1..1142
                     /locus_tag="VFI12_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056533.1"
                     /translation="EVQSQVASMMTDRAEPNASAAIMGGWRSFVFVGLTAAGFWAYGN
                     ARLELKAAGFALIALVAADLWSIEREYWMYMPPASVTFATDDAIEAIKKDIASTGEPG
                     RTILLFAGQGLSPNDAYFRKNALMNQLLRTVEGEQGNELDIYRRMVQLDSGNVVLNPT
                     FWRHENVRYLYTGADEATLAGAAQQLHVPPFVRLAGPVKNANGSTVYSYRVGPADPFA
                     WVASGAVRAQPEQVLPTVLDPRYNPAIAAIVDTGSTLPVKDASQLVPSTVTVKTTSYS
                     PGKIAVELSAPATDGSILVLSENWYPGWSARGGSASLPTARANYNLIGVSLPAGAKSI
                     ELAFADASYARGKVVTFVALFVAGVLLIAGVVIDRRRRVAEPAAA"
     gene            1154..1918
                     /locus_tag="VFI12_08730"
     CDS             1154..1918
                     /locus_tag="VFI12_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005936448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HET7056534.1"
                     /translation="MDVIPPAHPERRALDQARALIVVPTYNERENIARIIDAALAQDP
                     RLEMLIVDDGSPDGTAAIVEGIMERSARVHILKRAGKMGLGTAYLAGFRWALDRGYDL
                     ILEMDADFSHDPNHLPQFLHAAESADLVIGSRYRDGKVTVVNWPIARLLLSYYANVYA
                     RWVTGLPIWDCTAGFKCFHKKVLESINLNNVKANGYAFQIEMHFRTWKRGFKMVEIPI
                     VFVDRTEGTSKMSGSIVREAIWMVWRMRLLSLVNKT"
     gene            1918..2739
                     /gene="dapF"
                     /locus_tag="VFI12_08735"
     CDS             1918..2739
                     /gene="dapF"
                     /locus_tag="VFI12_08735"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HET7056535.1"
                     /translation="MSIAGREFIKMSGSGNDFVMVDARTEPAGELEDPRVIAGICARA
                     TGVGADGIVFLQASSKADIKLLYLNSDGSLADLCGNATLCTTRLAVELSAAERDNVSI
                     ETGAGIIHSRILPSGLPEIDLPGVLELQPELAGIPGEPSDRHLGFARAGIPHLVIEVP
                     DVSQVDVTGRGRPLRFHKSLRDGANVNFVSGSGDDWSIRTYERGVEGETLACGTGSVA
                     SAILIAIWHGGSGPIRLKTRSGRTLTVRLTRAGSGFVPSLSGEARIVFRGRFGEF"
BASE COUNT          500 a          866 c          899 g          519 t
ORIGIN      
        1 ccgaggtgca gtcacaggtc gcgagcatga tgaccgaccg cgccgagccc aatgcgtcgg
       61 cggcaatcat gggtggctgg cggtcgtttg tgttcgtcgg attgaccgcc gcggggttct
      121 gggcctacgg caacgcgcgg ctcgaattga aggcggcggg cttcgcgctc atcgcccttg
      181 tcgccgcgga tctctggagt atcgagcgcg agtactggat gtacatgccg ccggcatccg
      241 tcaccttcgc caccgacgac gcgatcgagg cgatcaagaa ggacatcgcg tcgacgggcg
      301 agccagggcg aaccatcctg ctgttcgccg gccagggcct cagtcccaat gacgcctatt
      361 tccgcaagaa cgccctgatg aaccagctcc tccggaccgt cgaaggcgag cagggaaatg
      421 agctcgacat ctaccggcgc atggtgcagc tcgattcggg caatgtcgtg ctgaatccca
      481 cgttctggcg tcatgaaaac gtgaggtatc tctacacggg agccgacgaa gccacgctcg
      541 ccggtgccgc gcagcagttg cacgtgccac cgttcgtcag gctggccggc cctgtgaaga
      601 acgccaacgg gtcgaccgtt tattcgtatc gcgtcgggcc agccgatccg ttcgcatggg
      661 tcgcaagtgg cgccgtacgg gcgcagccgg agcaggtcct tccgacggtg ctcgatccac
      721 ggtacaaccc ggccatcgcc gcgatcgtcg acacgggcag cacgctgcct gtgaaggacg
      781 catcacagct cgttccgtcg accgtgaccg tgaagacgac ctcgtattcg cccggcaaga
      841 tagcggtcga gctttcggcg ccggcaaccg acggctcgat ccttgtgctg tcggaaaact
      901 ggtatccggg gtggagcgcc cgcgggggat cggcgtcgct tccaacggcc cgcgcgaact
      961 acaacctgat cggcgtctcg cttccggccg gtgcgaagag catcgagctc gcgtttgcgg
     1021 acgcgtccta tgcgcgaggg aaggtcgtta cgttcgtagc cttattcgtg gccggagttc
     1081 tgcttattgc cggggtcgtc attgatcgcc ggcgcagggt cgccgaaccg gctgcggcct
     1141 gactttctga accatggacg tgataccgcc agcacacccc gaacgccgtg cgctcgacca
     1201 ggcgcgcgcg ctgatcgtcg tgccgacgta caacgagagg gaaaacatcg ccaggatcat
     1261 cgatgccgcc cttgcacagg atccgcggct cgagatgctg atcgtggacg acggatcgcc
     1321 tgatggaacg gcggccatcg tggaagggat catggagcgg tcagcccgtg tccacattct
     1381 caaacgcgcc gggaagatgg gcctcggcac ggcgtatctg gccggattcc gctgggcgct
     1441 cgacaggggc tacgacctca tcctcgagat ggacgccgac ttttcccatg acccgaacca
     1501 cctgccgcag ttcctccatg ccgcggaaag cgccgacctc gtgatcgggt cgcgctaccg
     1561 ggacggaaaa gtcaccgtcg tcaactggcc gattgctcgt ctccttctca gctattacgc
     1621 aaacgtctac gccaggtggg ttaccggtct cccgatctgg gactgcaccg ctggtttcaa
     1681 gtgcttccat aagaaagtac ttgaatcgat caacctaaat aatgtaaagg caaacggtta
     1741 cgcctttcag atcgagatgc acttcaggac gtggaaacgc ggcttcaaga tggtcgaaat
     1801 tcccatcgtg ttcgtcgacc gtactgaagg caccagcaag atgtccggct cgatcgtccg
     1861 ggaggcaatc tggatggtct ggcggatgcg tttgctctcg ttggtgaaca agacctgatg
     1921 tccatcgccg gccgggagtt catcaagatg agcgggtcgg gcaacgactt cgtgatggtc
     1981 gatgcccgga cggagcctgc tggcgagctc gaagaccctc gcgtcatagc agggatctgc
     2041 gcccgggcga ccggtgtcgg cgccgacggc atcgtctttc ttcaggcgtc cagcaaggcc
     2101 gacatcaagc tcctgtacct caactccgac gggtccctcg ctgacctctg cggcaacgcg
     2161 accctctgta cgacgcggct cgccgtcgag ctgtcggctg cggagaggga caacgtgtcg
     2221 atcgagaccg gggcggggat aatccactcg cgaatccttc cgtccggact tccggagatc
     2281 gatctgccgg gggtgctcga gcttcagccg gagctggcgg gaataccggg agagccctcg
     2341 gacagacacc tgggatttgc gcgggccggc atccctcatc tggtgatcga ggtaccagac
     2401 gtttcgcagg tagacgtgac cggtcgcggg agaccgctcc gcttccacaa gtcgcttcgg
     2461 gacggggcca acgtgaactt cgtttccggc tctggcgacg actggtccat ccggacctac
     2521 gagcgaggtg tggaagggga gactctggcc tgcggaacgg ggtcagttgc gtctgccatt
     2581 ctgatcgcga tctggcacgg cggctctggg ccaatccgcc tcaagaccag gtctgggcga
     2641 accctcacgg ttcggctcac ccgggccggc agcggctttg tgccgagtct gtcgggcgaa
     2701 gccagaatcg tctttcgcgg tcgattcggc gagttctgaa ggtcgcgacc ggggccagct
     2761 ggaccgctca cggttcctgg tgcc
//
LOCUS       DASSGX010000489         4940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_521189, whole genome shotgun sequence.
ACCESSION   DASSGX010000489 DASSGX010000000
VERSION     DASSGX010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4940
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521189"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(254..1471)
                     /locus_tag="VFI12_08740"
     CDS             complement(254..1471)
                     /locus_tag="VFI12_08740"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7056536.1"
                     /translation="MDASPISRRAAAFHDLHSFTVIRDFLWNSPYTERRTDPGIADFT
                     FGNPHDMPMPEYVDALRAAANPQDPDWFGYKDYVPAAQEAAAASLERLLRIPFAPEDI
                     LLTTGGFAAIATALKTAADPGDEVIYSLPPWFLYEGLCAEAGLTPVKVMIDLTTFDLD
                     VDAIAAAITPRTRVVIINTPNNPTGRIYPPETLRRLAAVLEEASVRYGRRISLISDEP
                     YNRIVFDGARFHSPAEFYPYTFIAYSYGKTHLAPGQRIGYIALPPTMPDREAVRAAIM
                     QLQIVAGWAFPNAIMQYALPRLEQFMIDVERLQRRRDRIVEALTEMGYPIHRPEGTFY
                     LWPRSPIGDDAAFARELAEHDIFVLPGHMFETPGFFRICLTANDEMIERSLPGFAAAI
                     TRVRAEQPAFIST"
     gene            1751..3082
                     /locus_tag="VFI12_08745"
     CDS             1751..3082
                     /locus_tag="VFI12_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056537.1"
                     /translation="MSKIRSLVLTLIIVALFAAQPAVLGARAYAQGGSQAAALTVDPT
                     QDASLPGKKKDPHPEPTDTPAPPPPEPTEPPKPPPQPTNTPRPAPTDTPQPHPTDPPP
                     PTDTPVPPPPTNTPEPPEPTNTPVPPPTNTPIPPPPPTETPIPPPPPTNTPVPPPPTS
                     TPIPPPPPTNTPTPVVLPTFPVPTVPPPPESSSSTGLIQRDGYGVALKCAYHPETDTT
                     NCRFTVTAVTGQPTYDSAVLTSALCTEAMDGTVSYVEPDLQSGAIGYRFDAGHNKLVL
                     VGKATVKGNAHYAVNVSGTTLKLTGPGISCGVSKSAGTMIIVAKYVCSVPAGDARTTA
                     PLVFTQPRNEPFQSPDGAAKCHAVGEGDDSFTLDHVDKVPSIGGLPTHANGRVRFLGV
                     RAGQYRLIEGATGDRSQSFSVPGTGIEIFHVIQFVVETRVGEIPGGIGLLR"
     gene            3204..4241
                     /locus_tag="VFI12_08750"
     CDS             3204..4241
                     /locus_tag="VFI12_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056538.1"
                     /translation="MSKHRTVPYLIAIIALLFATFGSLPAPARADHNEHHHGDDRKHD
                     GGKKEKPPPDPTIEVTVPPPPATSTPEPTETATPAPTPIDESTSPPPAIPTTAPQTQH
                     PPPAPFVDPSAYQVAVKCKYSAAKDATACRFAVRPEPGWVAADHAILRSTLCTEVMSG
                     DLGYVAFDPATGAQGYWFDQANDRMVLVGQVKVKGTAHYAVQVGGISIKLTGPGLSCG
                     ISKSAGAQIIVSKYICSVAADDHRAGSTTGFAPAPNMPFQPPEGADRCRHVAEGETSF
                     GLDFVDGVPSVGGLPTRANGRVRFTGVSPGRYRLLERASHTRSDVFALTGSDLVLFRV
                     IEYIPDTRAGN"
     regulatory      4419..4527
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            4624..>4940
                     /locus_tag="VFI12_08755"
     CDS             4624..>4940
                     /locus_tag="VFI12_08755"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7056539.1"
                     /translation="MAQIVSIRGVSKQFGESAALPVLDAIELTVQAGEIVAILGPSGC
                     GKSTLLRLIGGLDRPSAGEVWIGDRVVDRSDPRCAVVFQEPRLFPWKRVAANVAVGAR
                     RTSA"
BASE COUNT          826 a         1727 c         1604 g          783 t
ORIGIN      
        1 ccacctggac cctccgcatt gagcgcatgt aggggcgtgc gggggcacgc ccgcgcctcg
       61 ccgcatcgca catcgccatg taccggaccg gtcaccggca ccatgccggg ggaaccacgg
      121 cggatgcgcc cccgcacatc cctacaatgc gtcgcactcc cgcgtctccg cgcattcacc
      181 cctgctcctc gcacgtctcg ttcccggctg ctcgcctgct cgcccagggc ggaagcgctc
      241 caggctgctc gcctcaggtt gagataaacg ccggctgctc agcccggacg cgggtgatgg
      301 cggcggcgaa gccgggcagg ctgcgctcga tcatctcgtc gttggcggtc aggcagatcc
      361 ggaagaagcc gggcgtctcg aacatgtggc cgggcagcac gaagatgtcg tgctccgcca
      421 attcgcgcgc gaaggccgcg tcgtcgccaa tgggagagcg cggccagagg tagaacgtgc
      481 cctccggccg gtggatgggg taccccatct cggtgagcgc ctcgacgatg cgatcgcggc
      541 ggcgctggag ccgctcgacg tcgatcatga actgctcgag ccggggcaag gcgtactgca
      601 tgatcgcgtt cgggaaggcc cagccggcca cgatctggag ctgcatgatc gccgcgcgaa
      661 cggcttcgcg atcgggcatg gtcggcggca gggcaatgta gccgatccgt tgcccgggcg
      721 cgaggtgcgt cttcccatag gaataggcga tgaaggtgta cgggtagaac tcggccgggc
      781 tgtggaagcg ggcgccgtcg aagacgatcc ggttgtatgg ctcgtcggag atcagggaga
      841 tccgtcggcc gtatcggacc gatgcctcct cgagcacagc ggccagccgg cgcagagtct
      901 cgggcgggta gatgcggccg gtggggttgt tcggcgtgtt gatgatgacg acacgcgtgc
      961 ggggtgtgat cgccgcggcg attgcgtcga catcgaggtc gaaggtcgtc aggtcgatca
     1021 tgaccttgac gggcgtcagc ccggcctcgg cgcagagccc ctcgtacagg aaccagggcg
     1081 gcaggctgta gatgacctcg tcgcctgggt ccgccgccgt cttcagggcg gtggcgattg
     1141 ccgcgaagcc cccggtcgtc agcaagatgt cctccggcgc gaaggggatc cgcagcaacc
     1201 gctccagcga tgcggcggcg gcctcctgcg cggcggggac atagtccttg tagccgaacc
     1261 aatccgggtc ctgcgggttc gccgccgcac gcagggcatc gacgtactcg ggcatcggca
     1321 tgtcatgcgg gttgccgaac gtgaaatcgg cgatgccggg gtcggtccgc cgctccgtat
     1381 agggcgaatt ccagagaaag tcgcgaatga ccgtgaacga gtggaggtcg tggaaggcag
     1441 cggcgcggcg tgagatggga ctggcgtcca tggcggacct ccggagcgtt tgctgggcga
     1501 agcgacgttc ccagcctatc acccggcgca ataccgcgct ccggagccgg gtttcggccg
     1561 gactcccggc ccggagcgcc gtgttggcaa ggccacgaca accggttgac atgtttgcta
     1621 cgctaatggt cctacccgac aggatcggac gcctccggca gcactccgtg tttacggctc
     1681 cctccattcc tcatctgttg ccggaccgcg tcaccttggc ctgtcaccgc acccgcaaag
     1741 gcggtgctcc atgtcgaaga ttcgctcgtt ggtgctcacg ttgatcatcg tcgcgctgtt
     1801 cgcggcgcag cccgccgtgc tcggcgcccg agcctacgcc cagggcgggt cccaggcggc
     1861 tgcgctcacc gttgacccga cgcaagatgc gtcgctcccc ggcaagaaga aggacccgca
     1921 cccggagccg acggatacgc ctgccccgcc tccgcccgag ccgacggaac cgcccaagcc
     1981 gccaccgcag cccacgaaca cgcccaggcc tgctccaacg gacaccccac aaccgcaccc
     2041 aacggatccg ccaccaccga ctgacacgcc ggttccacca ccgccgacca acacgccgga
     2101 accgccggag ccgacgaata cgccggtgcc gccgcccaca aatacgccca tcccgcctcc
     2161 accaccgacc gagacgccga ttccgccacc gcctccgacg aacacacctg tcccgcctcc
     2221 accgacgagc acgccaattc caccgcctcc tccaaccaac acgccgaccc cggtcgtgtt
     2281 gccgacattc ccggtgccta ccgtcccgcc gccgccggag agcagcagct ccaccgggct
     2341 gatccagcgt gatggctatg gcgtcgcgct caagtgcgcc tatcaccccg agaccgatac
     2401 gacgaactgc cggtttaccg tcaccgccgt caccggtcag ccaacctacg actccgccgt
     2461 gctgaccagt gcgctctgca ctgaggcgat ggacggcacc gtctcgtacg tcgagcctga
     2521 tctccagtcc ggcgcgatcg gataccggtt cgacgccggg cacaacaagc tggtcctggt
     2581 cggcaaggcg acggtgaagg gcaatgcgca ctacgccgtc aatgtgagcg ggaccactct
     2641 caagttgacc ggccccggca tctcctgcgg cgtctcgaag agcgccggca cgatgatcat
     2701 cgtcgccaag tacgtctgct ccgttccggc cggcgatgcc cggacgacag cgccgctcgt
     2761 ctttacacag ccgcgcaatg agccgttcca gtctccggac ggcgcggcta agtgccatgc
     2821 cgtcggggaa ggggacgaca gcttcacgct ggaccacgtg gacaaggttc cctccatcgg
     2881 cgggttgccg acgcacgcca acggccgggt ccggttcctg ggcgttcgcg ccggtcaata
     2941 ccggctgatc gagggggcaa ccggcgatcg atcccagtcc ttcagtgttc cgggcaccgg
     3001 gatcgagatc ttccacgtga tccagttcgt ggttgaaacg cgtgtcggcg agatcccggg
     3061 gggcatcggg ctcctgcgct gatcgcacgc atggctattg gttgcctggg caccgttctc
     3121 gggtggaacg gttgctacgc tggcagttcc atcgcctgtc cggcgccatc ctcgcttgcg
     3181 ccgcgcaaac cggggcattg ctcatgagca aacaccgcac cgtgccgtac ctcatcgcga
     3241 tcatcgcgct cctcttcgcc acgttcggct cgctgccggc gccggcccgc gccgatcaca
     3301 atgagcacca ccatggtgac gaccggaagc atgacggcgg caagaaggag aaacccccgc
     3361 ccgacccgac gatcgaggtc accgtcccgc caccgcctgc tacgtcaact ccggagccga
     3421 cggagacggc aacacccgcg cccacgccga tcgatgagtc gacctcgccc ccgccggcaa
     3481 ttcccacaac cgcgccgcaa acccaacacc cgccgccggc tccgttcgtc gacccaagcg
     3541 cctaccaggt cgccgtaaag tgcaagtact ccgcggcgaa ggatgcgact gcctgccggt
     3601 tcgcagtccg gccggagccg ggttgggttg cggccgatca cgcgatcttg cggagcacgc
     3661 tctgcaccga ggtcatgagc ggcgaccttg ggtacgtcgc cttcgacccg gcaaccggcg
     3721 cgcagggcta ttggttcgac caggccaacg atcggatggt gctggtcggc caggtcaagg
     3781 tcaagggaac ggcccactac gccgtccagg tgggcgggat cagcatcaag ctcaccggac
     3841 cggggctctc ctgcggcatt tcgaagagcg ccggcgcgca gatcatcgtc tcgaagtaca
     3901 tctgctctgt tgctgccgac gatcaccgtg ccggcagcac gaccggattt gcaccggcgc
     3961 cgaatatgcc attccagccg cctgagggcg cggaccgctg ccggcacgtc gccgagggtg
     4021 agaccagctt cgggctggac ttcgtcgacg gggtgccatc ggtcggcggc ctgccgacgc
     4081 gtgccaatgg ccgggtccgc ttcaccgggg tcagcccggg ccgttaccgg cttctcgagc
     4141 gcgcctcgca cacgagatcc gatgtcttcg ccctcaccgg gagcgatctg gtcctgttcc
     4201 gggtgatcga atacatcccg gatacccgcg ccggcaactg aacactctgc tccgagccgt
     4261 tggcaggaat ccccggatcc gccatccaat gtagccttgg gcggcccgcc cggggcttca
     4321 tcgaggggcc agcgcaatcg ggttcggcgg ggttgccgct gccggttgtt gacattgttg
     4381 atcgactact ctagattacg cacacaaccg aagacgcaca ctcatccaga ggggcagagg
     4441 gaccggccca atgaagcccc agcaaccccc cggtcgcacg accgggatgg tgctaattcc
     4501 ggcagagatt gttctggaag atgagggttg cgccggcgcc gcgtcgatcc cagcgccgtc
     4561 tccgccccat cctccagatg gggcgtttcg catctccgcg agatgtcgag cagaggggga
     4621 gacatggcgc agatcgtttc catccggggt gtcagcaagc agttcggcga gtcagcggcg
     4681 ctgcccgtcc tcgacgcgat tgagctgacc gtccaggccg gcgaaatcgt ggccatcctc
     4741 ggaccgagcg gctgcgggaa atccacgctc ctgcgcttga tcggcgggct cgaccgaccc
     4801 tcagccggcg aggtctggat cggggatcgc gtcgtcgacc ggtctgaccc gcgctgcgcg
     4861 gtcgttttcc aggagccgcg cctgtttccg tggaagcggg tcgcggcgaa tgtcgcggtc
     4921 ggggcccgac ggacgagcgc
//
LOCUS       DASSGX010000490         5320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_850566, whole genome shotgun sequence.
ACCESSION   DASSGX010000490 DASSGX010000000
VERSION     DASSGX010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5320
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850566"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="VFI12_08760"
     CDS             complement(<1..862)
                     /locus_tag="VFI12_08760"
                     /inference="COORDINATES: protein motif:HMM:NF026042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7056540.1"
                     /translation="MFRCKVCGWIHDGIAPPNECPSCGATADRFRPMDAREINLTTNE
                     LASYGIVGQGLDIDVDDTRRIRLLAFVDASFFSPYTERTGKRYPLYTGDAQWFSDNIP
                     CMSACPTHTDISRYIALIADGRYADSYELNREHNVFPGCLGRMCARPCEQACRRKEVD
                     APIGICYLKRVASDYRGETRREIPPPWNGLTAGIVGSGVNGLSAARQLARKGYKVTIF
                     EKQPVPGGVMWTGVPEWRLPRDVIAEETQLIVDLGIDIHYNTEVGRDITLQEMHDRYD
                     VVVLCAGNQIA"
     gene            1150..1596
                     /locus_tag="VFI12_08765"
     CDS             1150..1596
                     /locus_tag="VFI12_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056541.1"
                     /translation="MRLGDLGDKLKQMLGQAQEKTADARSKATDMAGHAADQAKRTSQ
                     DLTEMAKSAAEQGKAKVDEMRSDASSAADSAKSQSDSVKSQAQATADDMKNQAKAAAD
                     DAKGAADSAKAAADDAKAHIDAAKAHSDAAVADAKAAVQDAKDKLS"
     gene            1687..2484
                     /locus_tag="VFI12_08770"
     CDS             1687..2484
                     /locus_tag="VFI12_08770"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7056542.1"
                     /translation="MAAERVLALEHVSVWTEDRTVILSDISWSVRAGEHWAVLGPNGA
                     GKTTLFTVATARRYPSRGTVEVLGRRFGEASMLELREQISIVDPHQRMYDWFTVEEIV
                     LTGVTGTVQPLPDRYTDEDRARAARIMEQVGLGGMADREIQSCSQGERQRVRVARALM
                     TRPRLLVLDEPASGLDLPAREALIASLTDLEKSDPDLASMMVSHHLEELPPTITHALL
                     LRDGRIVACGTVDDVLTDAAVSDAFGIPVRVSRQEGRWAARGVATWR"
     gene            2630..>5320
                     /locus_tag="VFI12_08775"
     CDS             2630..>5320
                     /locus_tag="VFI12_08775"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HET7056543.1"
                     /translation="MNPRLSPVVVRAPQDSGDPDQKATMPDDAGDLLPPDWGDHLIAI
                     GRAGLRASTLADARSAIVNGCVGLAGFDHAVLSLRGPHLESSAASSVLVFSEGLSLDD
                     AAAISVTFGDDDLAGGVQGEVTRDGRLLRIIRVSVETTPESSASLFLIQSGAAENSRG
                     DAQVARLAAEQVRLALRHATAEEDVRIQAFERRLLARLTDAIVGMQDLGSVMSEVAAA
                     VRVVSGWEGVAVVSYVRDADWLAVEATEGADGPTPALPQVGERLRLADWPSLRFALDN
                     CAPYQLTRDQAEALTVAERENVAMLGIESLLAVPFALNGEAVGLMLLYSGVARSLNAR
                     CLTSVQEIGVKAALAFQHNQYVGLARQQAIEQAALLRVSKAIISGQDLYAVLAEIARA
                     ALGFSGVEGCRVLLAHDDQERFEVGACQSVRDWPMYYQVSDTYPFADWPTSRAVLKDR
                     VARGYLVTDPELTARERANHVADQIQCLHRFPIVIGEDAVGVLTFMSRASRRFTPNTV
                     RIGHELAAQAAHAVDRARLFGKLQRRAETDGLTGLLNHRTAFETLDRELAAARKLDEP
                     LSIIIVDLDDFKLFNDTHGHLVGDRVLIEVSNALRDASRPRDYVARFGGDEFLLILPA
                     TSRSHAAQAAERILRRMSGTSVLVNTLELPIRISVGVATFPHDAGNRQELIAYGDAAM
                     YSAKECGGGQLGSIERRTRSLEVTVFGALSGLVRAVDRKDRYTKDHSDLVAEYSVRFG
                     KYLELSADELDALDVAGQLHDIGKIAVPDSVLRKPGQLTPEEEAMIRQHVVFSEMIIK
                     GIPNLDHVLAAVANHHERWDGSGYPYGKRGPGIPLLGRILTLADALAAMTLDRPYRKG
                     RTRDEAIAELRSGAGTQFDPNLVEDFIAAISTDTAILRDED"
BASE COUNT          917 a         1673 c         1836 g          894 t
ORIGIN      
        1 aagcgatctg gttgccggcg cagagcacga cgacgtcgta ccgatcgtgc atctcctgca
       61 gggtgatgtc gcggccgacc tcggtgttgt agtggatgtc gatgccgaga tcgacgatca
      121 gctgcgtctc ctcggcgatc acgtcgcgcg ggaggcgcca ctcgggcacg ccggtccaca
      181 tcacgccgcc ggggaccggc tgcttttcaa aaatggtgac cttgtagccc ttgcgggcga
      241 gctggcgggc ggcgctcagg ccgttgacgc cgctgccgac aatgccggcg gtcaatccgt
      301 tccagggcgg cggaatctcg cgccgggtct cgccgcgata atcggacgca acgcgcttca
      361 ggtagcagat gccgatcggg gcgtcgactt ccttacggcg gcaggcctgc tcgcaggggc
      421 gggcgcacat ccgacccagg cagcccggga agacattgtg ctcccggttc agctcatagc
      481 tgtcggcata ccgcccatcg gcgatgagcg cgatgtagcg ggaaatgtca gtgtgcgtcg
      541 ggcaagcgga catgcacggg atgttgtccg agaaccactg cgcgtcgcct gtgtagagcg
      601 gataccgttt tccggtccgt tccgtatacg gcgagaagaa cgaggcatcg acgaacgcca
      661 gcagccgtat gcggcgcgtg tcatcgacgt cgatatccaa cccctggccg acgatcccgt
      721 agctggccag ctcgttggtg gtcaaattga tttcacgggc gtccatgggc cggaagcggt
      781 ccgccgttgc gccgcaggac gggcactcgt tcggcggcgc aatgccgtca tggatccagc
      841 cgcaaacctt gcatcgaaac atggtagatc tgtgctcccc gcggaagcca acgtggcata
      901 cccgcgcagc gatgacgatc gcacgcgtat ggtccagtat agccgcgtcg tcaggggata
      961 cggaacggcg ggcagcgaga gtagggtagg gcttccgcga tccgggttgc tatgcgcctg
     1021 tcgcgccagt gtggccccga ttccgtttat ttatgtgcat ttcggggtat tcttggcgca
     1081 gatgcccgcg caactcggaa tgtgagtcag cgtggtgtac ggaatccggt tcgaccaggg
     1141 ggaagtacga tgcgactcgg ggatctcggc gacaagctga agcaaatgtt gggacaggcg
     1201 caagagaaga cggctgacgc ccgctcgaag gccaccgaca tggcgggcca tgccgcggat
     1261 caggccaagc ggaccagcca ggacctgacc gagatggcga aatcggcggc cgagcagggc
     1321 aaggccaagg tcgacgagat gcggagcgac gcatcgagcg cggccgacag tgccaagtcc
     1381 cagtcggact cggtgaagag ccaggcgcaa gccaccgccg acgacatgaa gaaccaggcc
     1441 aaagcagccg ccgacgatgc caagggcgcg gcggactcag ccaaagcagc cgccgacgat
     1501 gcgaaggcgc acatcgatgc ggccaaggcg cacagcgacg cggccgtggc cgacgccaag
     1561 gcggcggtgc aggatgcaaa ggacaagctc tcctagtcat tcccggctgc gataattcga
     1621 tctgaacctg acacggttgg acagcatgtg ctgtccaacc gtctttgtgc ggcaaggatg
     1681 agcgagttgg cggcggaacg ggtattggca ctcgagcacg tgtcggtctg gacggaggac
     1741 cgcacggtca tcctctccga catctcgtgg agcgtgcggg cgggggagca ttgggcggtg
     1801 ctcggtccga acggcgcggg caagacgacc ctgttcaccg tcgcgaccgc gcggcgctat
     1861 ccctcgcggg ggacggtgga ggtcctcggc cgccgcttcg gcgaggcgag catgctcgag
     1921 ctgcgcgagc aaatcagcat cgtcgatccc catcagcgca tgtatgactg gttcacggtc
     1981 gaggagatcg tcctgaccgg cgtcaccggc acggtccagc cgttgccgga tcgatacacg
     2041 gacgaggacc gggcccgagc cgcgcggatc atggagcagg tcgggctggg tggcatggcg
     2101 gaccgcgaga ttcagtcctg ctcccagggc gagcgccagc gcgtgcgcgt ggcgcgggcg
     2161 ttgatgaccc ggccccggct gctggtgctg gacgagccgg ccagtgggct cgatttgccg
     2221 gcccgggagg cgctgatcgc gtcgttgacg gaccttgaga agtcggatcc ggacctggcc
     2281 agcatgatgg tttcgcacca tctcgaagag ctgccgccga cgatcaccca cgcgctgctc
     2341 ctgcgcgacg ggcgcatcgt ggcctgcggc acggtcgatg acgtcctgac cgacgcggca
     2401 gtatccgacg ccttcggcat cccggtgcgg gtgtcccgtc aggagggccg atgggcggcc
     2461 cgcggcgtcg cgacctggcg ctagcaatgc tctgagcgag gcgccgcgtc cccgtttcct
     2521 cctgtgacgt tggtacccag aactctggcg aaatcctccc atgggattgg gcaggaccga
     2581 ttcctaagat gaatcaatga acacctgcga cccgcgcaag gattgacgat tgaacccgcg
     2641 actttcgccg gtggtagtgc gcgctccgca ggactccggc gatccagatc agaaggcaac
     2701 gatgccggac gatgccggtg acctgctgcc gcccgattgg ggagatcacc tgatcgccat
     2761 cggccgggcg ggcttgcggg cgtcaacact cgctgacgcc agatctgcga tcgtcaatgg
     2821 ctgcgtgggt cttgcagggt tcgaccatgc ggtgctcagt ctccgtggcc cacacctgga
     2881 gtcgagcgcc gcgtcgtccg tgcttgtctt ttccgagggc ttgtccctgg acgacgctgc
     2941 agccatctcg gtgacattcg gcgacgatga tctggctggc ggcgtccagg gcgaggtaac
     3001 ccgggacggg cgtctgttgc ggatcatccg cgtctccgtc gagacaaccc cggagtcctc
     3061 agcatcgctg ttcctgattc agagcggcgc ggcggaaaac tcgcggggtg acgcgcaggt
     3121 agcccggctg gccgccgagc aagttcgcct cgccctgcgc catgcgaccg cggaagagga
     3181 cgtccgaatc caggcgtttg agcgcagact gttagctcgg ttgaccgacg cgatcgtcgg
     3241 catgcaggac cttgggtcgg ttatgtccga agtcgcggcc gcggttcgcg tggtctccgg
     3301 ctgggaaggg gtcgcggtcg tgtcgtacgt ccgcgatgcc gactggctgg cggtcgaggc
     3361 gaccgagggc gccgatggac cgacgcccgc tctgccacag gtcggcgagc ggcttcgcct
     3421 ggccgattgg ccgagtctgc ggttcgcgct cgataattgc gctccgtacc aattgacccg
     3481 ggaccaggcc gaagcgttga cggtcgccga gcgcgagaac gtcgccatgc ttggcatcga
     3541 gtcgctcctc gccgtgccgt ttgccctcaa cggtgaggca gtcggcctga tgttgctgta
     3601 ttccggcgtg gcgcggtcgc tcaatgcgcg ctgtttgacg agcgtgcaag agatcggggt
     3661 caaggcggca ctggcgttcc agcacaacca gtatgtcggg cttgcccggc aacaggcgat
     3721 tgagcaggcc gcgctcctgc gcgtctcgaa ggcaatcatc tcgggccagg atctctatgc
     3781 cgtgctggct gagatcgccc gcgccgcact cggattctcg ggggtcgaag gctgccgggt
     3841 gctgcttgcg cacgacgatc aggagcggtt cgaggtcggg gcctgccaga gcgtgcgcga
     3901 ctggccgatg tactaccagg tctcggatac ttatccgttc gccgactggc ccacgtcccg
     3961 tgccgttctc aaagacaggg tggcgcgtgg gtacctggtc actgatcccg aactgacggc
     4021 tcgcgagcgg gcgaatcacg tcgcggacca aatccagtgt ttgcaccggt tcccgatcgt
     4081 gatcggcgag gacgccgtcg gcgtgctgac cttcatgagc cgtgccagcc ggcggttcac
     4141 gcccaacacc gtccggatcg gacacgagct ggctgcgcag gccgcccacg cggtcgatcg
     4201 cgcgcggctc ttcggcaagc tccagcgccg ggcggaaacc gacgggctaa ccggcctcct
     4261 caatcaccgc acggcctttg aaacgctcga tcgggagctg gcggccgcgc gaaagctgga
     4321 cgagccgctc tcgatcatca tcgtcgacct ggacgacttc aagctgttca acgacacgca
     4381 cggccacctg gtcggcgacc gggtcctgat cgaagtctcg aatgcgctgc gggacgcatc
     4441 gcggccgcgc gattacgtgg cgcggttcgg tggggacgaa tttctcctga tcctgccggc
     4501 aaccagccgg agccatgccg cgcaggcagc ggaacggatc ctgcggcgca tgtccggcac
     4561 atccgtgctg gtcaacacgc tcgagttgcc gatccggatc tcggtcggcg tcgcgacctt
     4621 cccgcacgat gccggtaacc gccaggagct gatcgcatac ggcgatgcgg caatgtactc
     4681 ggccaaggag tgcggcggcg ggcagctcgg cagcatcgag cggcgaaccc gatcgctcga
     4741 ggtgacggtc ttcggcgcgc tcagcggcct ggtccgggcg gtcgaccgga aggatcgcta
     4801 caccaaagat cactccgacc tcgtcgcgga gtacagcgtc cgctttggga agtacctcga
     4861 gctttccgcg gatgagctcg acgcgctcga cgtagccggc cagttgcacg acattggcaa
     4921 gatcgccgtg ccggactcgg tcctccgcaa gccgggccag ctcacgcccg aggaagaggc
     4981 gatgatccgg cagcatgtcg tgttcagcga aatgatcatc aagggaatcc cgaatctcga
     5041 tcatgtgctg gcggcggtag cgaaccatca tgagcgctgg gatggcagcg gctacccgta
     5101 cgggaagcgc ggcccgggca ttccgttgct gggccgaatc ctgaccctgg ccgatgccct
     5161 cgcggcgatg acgctcgatc gcccgtaccg gaagggccgc acccgggatg aggcgatcgc
     5221 cgagctgcgg agtggagccg gcacgcagtt cgaccccaat ctggtcgagg acttcatcgc
     5281 ggcgatctcg accgacaccg cgatcctgcg ggacgaagac
//
LOCUS       DASSGX010000491         2853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_959613, whole genome shotgun sequence.
ACCESSION   DASSGX010000491 DASSGX010000000
VERSION     DASSGX010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_959613"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /gene="rsgA"
                     /locus_tag="VFI12_08780"
     CDS             complement(<1..506)
                     /gene="rsgA"
                     /locus_tag="VFI12_08780"
                     /inference="COORDINATES: protein motif:HMM:NF015170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase RsgA"
                     /protein_id="HET7056544.1"
                     /translation="MRSHLGWWLAIETVLTGTVVKAYGKFFEVALDGTDRSLLATPKG
                     LLKRERKGTDIVAVGDRVTVIDVGDNEGRIESVAPRTSVLSRLARHTRDVEQVLVANP
                     DQALFLFAATEPTPHPRMLDRFLVLAESRDLPALIGVNKVDLLAGDPTRIFSEHRRIY
                     PVIDLSVRT"
     gene            649..1566
                     /locus_tag="VFI12_08785"
     CDS             649..1566
                     /locus_tag="VFI12_08785"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056545.1"
                     /translation="MTQPERIVAAIDIGSNTLKLTVAKVGNGLVKPVYGDAQVVRLST
                     GLAETGRLREDRIELAITALRAFTEEARANRATEITAVATEATRSAENGPDFLDRVRA
                     EVGIDVQVIDGDEEARLTSDGVLAQIDRSGRILIADIGGASTELIATRDGNVLGTVSL
                     GIGSGIMTDQIVPSDPPRAGELDRVEQAAADLAGAFLADRGEFDRIILVGGVGSFLLA
                     LVDKVGIVPPSVLDDARRITQAVSSHALAPAVNAPVARARVLPAGFAIARAICRLSAA
                     PAIESVANGLRIGILLRIAGSGAREETPR"
     gene            1563..2738
                     /locus_tag="VFI12_08790"
     CDS             1563..2738
                     /locus_tag="VFI12_08790"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET7056546.1"
                     /translation="MTSSQRIVIIGAGVIGTSVAAHLAKRGERPLVLERDHICSGTTG
                     QSGGLVRQHYSNPETATLARDALQIFRHWADHFEGTSGFIPVGVLLTAGPETEASVRA
                     NVAMHQGLGIETTLLEPGDIARIDPRVRVDDCTTICYEPTAGIADPIETTHAYAATAR
                     SLGVEIREGVSVSEIVVESGRVTGVRASDGIIPADVVINAAGAWGTSLMATLGHELPI
                     TFSRHPMALIRRPPAEIDRHPAVLDVHTDSYFLPRADATLVGKLGTMPADMDTDPNRY
                     NRGVTNEEIARYRDAARRRMPFLDRGVVLGGWAGIYDDSIDAHPIVDAVPGADGLFCA
                     LGMSGNCFKLSPVIGDLLAQRIISGPGSVPALDLFRYDRFREGSTHDRAFGAMSVLA"
BASE COUNT          504 a          979 c          923 g          447 t
ORIGIN      
        1 gtgcggacac taaggtcgat cacgggatag atccggcgat gctcggagaa gatccgggtc
       61 ggatcgccgg ccagcagatc gaccttgttg acgccgatca gggccggcaa atcgcgcgac
      121 tcggccagaa ccaggaaacg atccagcatg cgcgggtgcg gcgtcggctc ggtggctgcg
      181 aacaggaaga gcgcctgatc ggggttggcg accagcacct gctcgacgtc gcgggtgtgc
      241 cgggccaagc gcgacagcac ggacgtgcgc ggcgcgacgg actcgatccg gccctcgtta
      301 tcgccgacgt cgatcacggt cacccggtcg cccaccgcga cgatatccgt tcctttccgc
      361 tcccgcttca gcagcccttt gggggtagcg agcagcgacc ggtcggtccc gtcgagggcc
      421 acctcgaaga acttgccata tgccttgacg accgtgccgg tcaggacagt ctcgattgcc
      481 aaccaccacc ccaaatgtga acgcatcgca ggagttgctc ctcaattatc atcacgtttg
      541 caacaaccgg ccgatgacag gtgaacgcgg aatgcgcgac agcaacaagg gacgaaaatc
      601 gacccgcttc gatgcgtccg ccagcccggt agggacagcg gctcgcccat gacacagccg
      661 gagcggatcg tcgccgcgat cgacatcggg tcgaacacgc tcaagctcac cgtggcgaag
      721 gtcggaaacg gcttggtgaa accggtctac ggcgatgccc aggtcgtccg cctcagcacc
      781 ggactggcgg agaccggccg cctgcgcgag gaccggatcg agctcgcgat cactgcactc
      841 cgcgcgttta ccgaagaagc gcgagccaac agggccaccg agatcacagc cgtcgccacc
      901 gaagcaaccc gctcggccga gaacgggccg gatttcctgg accgggtcag ggccgaagtc
      961 ggcatcgacg ttcaggtcat cgacggtgat gaagaagcgc ggctgaccag cgacggtgtc
     1021 cttgcccaga tcgaccggtc cgggcgaatc ctgatcgccg atatcggcgg ggccagcaca
     1081 gaattgatcg ccacccggga cggcaacgtg ctgggcaccg tctcactcgg catcggttcc
     1141 gggatcatga ccgaccaaat cgtgcccagc gatccgcccc gagccgggga actggatcgc
     1201 gttgagcagg cagcagccga cctggcgggg gcgtttctcg ccgatcgtgg cgagttcgat
     1261 cggatcatcc ttgtcggcgg tgtcgggtct tttctccttg cgctcgtcga caaagtcggg
     1321 atagtccccc cgagcgtgct cgacgacgcg cggcgaatca cccaagcggt ttcatcacat
     1381 gccctggctc cggccgtcaa tgccccggtt gcccgggcgc gcgtcttgcc ggccgggttc
     1441 gccattgccc gggcaatctg ccggctatcg gctgcgccgg ccatcgaatc tgtcgcaaat
     1501 gggctgcgga tcggaatcct gttgcgtatt gcaggatccg gagcccggga ggaaacaccg
     1561 cgatgacatc gtcgcagcgc atcgtcatta tcggagccgg cgtcatcggg acgagcgtcg
     1621 ccgcccacct cgccaagcgc ggcgagcggc cgctggtcct cgagcgcgac cacatctgct
     1681 ccgggacaac cggccaaagc ggcggtctgg tccggcagca ctactcgaac cccgagacgg
     1741 ccacgctggc ccgcgacgcg ctgcaaatct ttcgccactg ggccgaccac ttcgagggaa
     1801 cgagcggctt catccccgtc ggcgtcctgc tcacggcggg accggaaacg gaagcgagcg
     1861 tgcgcgccaa cgtggccatg caccaggggc tcggcatcga gacgacgctg ctcgagcccg
     1921 gcgacatcgc ccggatcgat ccccgggtgc gcgtcgacga ctgcacgacg atctgctacg
     1981 aaccgaccgc cggcatcgcc gatccgatcg agacgacgca cgcctacgcc gccacggcac
     2041 gctccctcgg cgtcgagatc cgcgaggggg tctccgtctc ggagatcgtc gtcgaatccg
     2101 gccgggtgac cggcgtgcgg gcgagcgacg gcatcattcc cgccgacgtc gtgatcaacg
     2161 cggccggcgc ctggggaacg tccttgatgg cgaccctcgg ccacgagctg ccgatcacct
     2221 tctcccggca cccgatggcc ctgatccggc ggccaccggc ggagatcgac cgccatccgg
     2281 ccgtgctcga cgtccacact gattcctact tcctgccccg ggccgatgcg acactggttg
     2341 gcaagctcgg caccatgcct gccgacatgg acaccgaccc caaccgctac aatcgcggcg
     2401 tcaccaatga ggagatcgcg cgctaccggg acgccgctcg ccgacgcatg ccgttcctgg
     2461 accgcggcgt cgttctcggc ggctgggccg gcatctatga cgactcgatc gacgcgcacc
     2521 cgatcgtcga tgcggtccca ggcgcggacg ggctcttctg cgcgctcggg atgagcggga
     2581 actgcttcaa gctctcgccc gtcatcggcg acttgctcgc gcagcggatc atcagcggtc
     2641 ccgggtcggt tccggcactc gacctgttcc ggtacgaccg cttccgcgag ggatcaacgc
     2701 acgatcgtgc gttcggcgcg atgagcgtgc tcgcctgacc tttctccgcc cttccatgca
     2761 cagattagga gcagacgtgg atcagaccat cgttcagtcg atcgtggaga cggtcgaccg
     2821 gctggaatcg gaactggtgg agcggctgca gga
//
LOCUS       DASSGX010000492         3738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_739913, whole genome shotgun sequence.
ACCESSION   DASSGX010000492 DASSGX010000000
VERSION     DASSGX010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_739913"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..2213
                     /locus_tag="VFI12_08795"
     CDS             270..2213
                     /locus_tag="VFI12_08795"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7056547.1"
                     /translation="MVRLIPQFRGAIDPESLNDINEVIQARIDGGISRRQLIKRATQL
                     GIAAPVVGVMLHATSDMAFGQPSSGRDRTITLMQDTGTKEVTGPTQPAGTPKEGGVLV
                     ASTIEEPDTLNVWTTMLVTGFDVCVGIFEGLLQYDSNQQMVPRLADSFEISADGLTYT
                     WKLKSGITFHNGEPFNAQTVASNWRAIMTPEYGAWSQLGWDQITAVDTPDEATVVMKT
                     TEPYAPFVSYVGGGSWIIPASITDPAPPVAVAATPEGAAATPVASVPESAIEAFKALG
                     RNPIGTGPFKFDEWKSKEQISLSRYDGYWGSKAKLDQIIYRINPDDNTQLVQLRTGEA
                     QMAAGSGAVGSARLDEVLKYGTVDVYERPNMSWNHLDLKQLDFLRITKVRQALDFATP
                     TQDIIDKIMKGRVVRAVADQAPITAVYNPNVQPRPYDLDMAKQLLAEAGLTQNGDGIW
                     EGPTPTPSKDIDPNTDRGGPVKPFEMQFWYLSGDSTTQQIAQVIVQSWNSIGIKTEAK
                     SEDVSTIWGPEGYQWEPEHMTACMYSWSNSNDPDNAYYWNSSQIPPDPTGAGGNALCY
                     FYPFSFQDKVDEMTEAGARETDPEKRKAIYMELQQLIADEVPCIFLWWSKDYSAIMPT
                     IGGFWPSPFNNLFWNAQDWYLAE"
     gene            2268..3218
                     /locus_tag="VFI12_08800"
     CDS             2268..3218
                     /locus_tag="VFI12_08800"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7056548.1"
                     /translation="MNAFVIRRLLGAIPLLLGVSVLSFIFMQLAPGGPDTLLAKNGRM
                     TQAQLAAIRHNLGLDEPVHVQLWKWIVNLAQGDLGTSFTQQRPVRDVIFEVFPNTVYL
                     MVVALAISLLIAMIIGIGGAINQYGWFDNVTSFLAYFGLSMPVFWFGLMLQILFAVKL
                     GWLPSAGMHSATGSGAWDLIKHMVMPATTLAIGSVGSWSRYLRSSTLEIMGKDYVRTA
                     RAKGLRERVVMIRHVLRNAMIPFLTVVAIDIPLYLTGAVVTETVFSWPGMGRLFFDSL
                     TKRDYPVLMGILLLGAVLIVIGNLLADVLYAVLDPRISLK"
     gene            3215..>3738
                     /locus_tag="VFI12_08805"
     CDS             3215..>3738
                     /locus_tag="VFI12_08805"
                     /inference="COORDINATES: protein motif:HMM:NF024313.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7056549.1"
                     /translation="MSAAETTLASVEPRGMQQVSAKPLSPWQIVWRRLKRSRSALVGS
                     IVFGLVVLAAMFAGVIAPYGKNDIDLFNITAPASWSHWLGTDPLGRDVLTRLMYGGQY
                     SLLIGVGSAVIACTIGIVVGAVAGYYGGWVDAVLMRFVDLMLAFPSIFLLLIVAAILD
                     GITVTNVIIFLAAFG"
BASE COUNT          663 a         1173 c         1213 g          689 t
ORIGIN      
        1 gcggcgtcgg acggccggct gggtattggg aacacgtcgt actcggacga tcctcgccct
       61 acgggcgatc gccgggcgcg gaggcccccg cgctacattg tcgtcgccgg cctgagttct
      121 ggtatggcga aacgatggct ctcgacgcga aaggatttgc aaagtaggct accgcctatc
      181 tcagcaactg tcctgcgcaa cgtgcgtgag ggagtggagc cgccaccgcc ggtgatccgg
      241 cgggtggggc cagtgcaagg agtacacacg tggtccgact gattccccag ttccgcggag
      301 cgatcgatcc ggagtcgctg aatgacatca atgaggtcat ccaggcccgg atcgacggcg
      361 gcatttcccg ccggcaactg atcaagcggg cgacgcagtt gggcatcgcc gcgccggtcg
      421 tcggcgtgat gctgcacgcg acctcggata tggccttcgg ccagccgtcg tccggccggg
      481 accggacgat caccctgatg caggacacgg gcaccaagga agtcaccgga ccgacccagc
      541 cggccggcac gcccaaggag ggtggtgtcc tcgtcgccag cacgattgag gagcccgaca
      601 cgctcaatgt ctggacgacc atgctcgtga ccggcttcga tgtctgcgtg ggcatctttg
      661 agggactgct gcagtacgac agcaaccagc agatggtgcc gcgcctcgcc gacagcttcg
      721 aaatcagcgc ggacggcctc acctacacct ggaagctgaa gtcgggcatc acgttccata
      781 atggcgagcc gttcaatgcc cagacggtcg ccagcaactg gcgcgccatc atgactcccg
      841 aatatggcgc ctggtcacag ctcggctggg accagatcac cgcggttgac acgccggatg
      901 aggcaacggt cgtgatgaag accacggagc cgtatgcccc gttcgtctcc tatgtcggcg
      961 gtggctcctg gatcattccg gccagcatta ctgatcccgc cccgcccgtc gcggtcgccg
     1021 cgacgccgga gggagcggcc gcaaccccgg tcgcctctgt gccggaatcg gcgatcgagg
     1081 cgttcaaggc gctcggccga aacccgattg ggactggccc attcaagttc gacgagtgga
     1141 agtcgaagga gcagatttcg ctctcgcgct acgacgggta ctggggtagc aaggcgaagc
     1201 tcgaccagat catctatcgc atcaacccgg atgacaacac ccagctcgtc cagctccgga
     1261 ccggcgaagc gcagatggcg gccggctctg gtgcggtcgg ctcggcgcgg ctggatgagg
     1321 tgctcaagta cggcacggtc gatgtctacg agcggccgaa catgtcctgg aaccacctcg
     1381 acctcaagca gctcgatttc ctgcggatca caaaggtccg ccaggcgctc gatttcgcga
     1441 cgccgacgca ggacatcatc gacaagatca tgaagggccg cgtcgtgcga gcagttgccg
     1501 accaggcgcc gatcaccgcc gtctacaacc cgaatgtcca gccgcgccca tatgacctcg
     1561 atatggccaa gcagttgctg gccgaggccg gtctgaccca gaacggcgac ggcatctggg
     1621 aagggccgac gccgacgccg tcgaaggaca tcgatccgaa cacggaccgc ggtggcccgg
     1681 tcaagccgtt cgagatgcag ttctggtacc tctcgggtga ctcgaccacc caacagatcg
     1741 ctcaggtcat cgttcagtcg tggaactcga tcggcatcaa gaccgaggcc aagagcgagg
     1801 acgtctcgac catctggggt ccggaaggct accagtggga gccggagcac atgaccgcct
     1861 gcatgtactc ctggtccaac agcaacgatc cggacaacgc ctactactgg aacagcagcc
     1921 aaatcccgcc ggatccgacc ggtgcgggcg gcaatgcgct ctgctacttc tacccgttca
     1981 gcttccagga caaggtcgac gagatgaccg aggctggggc gagggagacg gacccggaga
     2041 agcgcaaggc tatctacatg gagctgcagc agctgatcgc ggatgaggtg ccctgcatct
     2101 tcctttggtg gagcaaggat tattcggcga tcatgcccac gatcggcggc ttttggccga
     2161 gcccgttcaa taacctgttc tggaatgcgc aggactggta cctggcggag tagcactgca
     2221 cgttaccggg ccggcgcatc tgccggcccg gtaccctgtg agcatcaatg aacgcgtttg
     2281 tcattcgccg gctgctggga gcgattccgc tcctgctcgg cgtatcggtt ctctccttca
     2341 tcttcatgca gctcgcgccg ggcgggccgg ataccctgct ggccaagaac ggccggatga
     2401 cgcaggcgca gctggcggct atccggcaca acctcgggct ggatgaaccg gtccatgtcc
     2461 agctctggaa atggatcgtc aacctggcgc agggcgatct ggggacgagc ttcacccaac
     2521 agcgtccggt ccgggacgtg atcttcgagg tcttcccgaa tacggtctat ctgatggttg
     2581 tggcgctggc gatctcgctc ctgatcgcga tgatcatcgg gatcggcgga gcgatcaacc
     2641 agtacggctg gttcgacaat gtcacctcgt tcctggccta cttcggactc tcgatgccgg
     2701 tcttctggtt cgggctgatg ctccagatcc tgttcgcagt gaagcttggt tggttgccct
     2761 cggccggcat gcacagcgcg acgggaagcg gcgcgtggga cctgatcaag cacatggtca
     2821 tgccggcgac gacgctcgcg atcggcagcg tcgggagttg gagccggtac ctgcggtcgt
     2881 ccacgctcga gatcatgggc aaggattacg tccgcacggc gcgggccaaa gggttgcgag
     2941 agcgggtggt gatgatccgg cacgtgctcc ggaacgcgat gatcccgttc ctgacggtcg
     3001 tcgcgatcga catcccgctc tacctgaccg gcgcggtcgt gaccgaaacg gtcttctcct
     3061 ggccggggat gggccggctc ttcttcgact cgctgaccaa gcgggactac ccggtcctga
     3121 tgggcatcct cctgctcggc gcggtcctga ttgtgatcgg taacctgctc gcggacgtcc
     3181 tctacgccgt tctcgatccc cggatttcgc tgaaatgagc gcagcggaaa cgaccctcgc
     3241 atctgtcgag ccgaggggca tgcagcaggt ttcggccaag ccgttgtcgc cgtggcagat
     3301 cgtctggcgg cggctcaagc gctcccgcag cgcgctggtc ggctcgatcg tcttcgggct
     3361 ggtcgtgctc gcggcgatgt tcgcgggtgt gatcgctccc tatggcaaga acgatattga
     3421 tctcttcaac atcaccgcgc cggcgagctg gtcgcactgg ctgggaactg acccgctcgg
     3481 ccgtgacgtc ctgacgcgcc tcatgtacgg cgggcaatac tcgctgctga tcggcgtggg
     3541 ctcggccgtg atcgcctgca cgatcgggat cgtggtcggg gcagtcgccg ggtattacgg
     3601 cggctgggtc gacgcggtgt tgatgcggtt cgtcgacctg atgctggcct tcccgagcat
     3661 cttcctgctg ctgatcgtgg cggcgatcct ggacgggatc accgtcacca atgtgatcat
     3721 cttccttgct gcgttcgg
//
LOCUS       DASSGX010000493         2432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1613087, whole genome shotgun sequence.
ACCESSION   DASSGX010000493 DASSGX010000000
VERSION     DASSGX010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1613087"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VFI12_08810"
     CDS             <1..382
                     /locus_tag="VFI12_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HET7056550.1"
                     /translation="VTRSMRAAIDETYRRRALQTEYNEAHGIQPRGIVKEIKDLTDRV
                     RAAAEESASYDAGAASKAGVIAELPKDEVLRIVKDLESQMKIAARDLEFEKAALLRDQ
                     IVELRRMMEVDPIAASVRPASGAM"
     gene            386..1378
                     /locus_tag="VFI12_08815"
     CDS             386..1378
                     /locus_tag="VFI12_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056551.1"
                     /translation="MKLVRFLPAFWVALVALLAFQLAGPAQTYGTTVPVTPTPDGSDN
                     KGSNSELSPELPPPDFPTANVEGYTFDLRYALSAELDKVPREAAVYELLRDEPTVAGV
                     QKIADKLGIDGKAEDRGDGSFTASGNGELFVAIDQVNYFSDVDVAQGDLPSDDKAINE
                     ARDWLRHASLLPPDIGKGSIVGRFPDSNRMVVSFAPAEPENILSGYPSIGLTIGADGA
                     VIEASIRWANIVRADVYQLMSTDQAWQLVSSGQAYLAPDLTQAKIDPGTDVKGRVTFT
                     SIKIAYATAGPPGGRQFLQPIYVFEGRIRPEGDKRTYPMTAYVSALSNSGAPVG"
     gene            1404..1850
                     /gene="aroQ"
                     /locus_tag="VFI12_08820"
     CDS             1404..1850
                     /gene="aroQ"
                     /locus_tag="VFI12_08820"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HET7056552.1"
                     /translation="MAKRILVLNGPNLNMLGTRQPEIYGRTSLADIEVAMRSVAQSVR
                     SDVELSFFQSNHEGALIDVIQQSAGKVDAVIINPGGLTHTSVALRDALVALDRPVVEV
                     HLTNIHAREPFRHHSYIAPIAIGQIAGLGAAGYLLAVRYVVETVLA"
     gene            1847..>2432
                     /locus_tag="VFI12_08825"
     CDS             1847..>2432
                     /locus_tag="VFI12_08825"
                     /inference="COORDINATES: protein motif:HMM:NF013486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HET7056553.1"
                     /translation="MSSNRIEQVRATLAELGTGALILTSPANRRWATGFTSAAGVPFS
                     TDLAIITPSAVELLVAPIHVGWARGESGVATDVRAHKGQFASSFADYLSERGLTHISL
                     EADALPYPEALKIQDATPVVAIEFVTGLRDTWRASKDPDEIASLERAARITDEVFIEM
                     SGWIAPGMDETSVARRISSQLIERSDGLAFDVIVA"
BASE COUNT          436 a          774 c          802 g          420 t
ORIGIN      
        1 tgtgacccgc tcgatgcggg cggcgatcga cgagacctat cgccggcgcg cgctccagac
       61 cgaatacaac gaggcccacg gtatccagcc gcgcggcatc gtcaaggaga tcaaggacct
      121 gaccgatcgg gtcagggcgg cagccgagga atcggcgtcg tacgatgccg gggccgcatc
      181 caaggccggc gtgatcgccg aactgccgaa ggacgaggtg ctacggattg tgaaggatct
      241 cgagagccag atgaagatcg ccgcgcgcga tctcgaattc gagaaggctg ccctgctccg
      301 cgaccagatc gtggagctgc ggcgcatgat ggaggtggac ccgatcgcgg catcggtccg
      361 ccccgcgtca ggagcaatgt agttcgtgaa gttggttcga ttccttccgg cattctgggt
      421 agcactggtg gcgctgctcg cgttccagct cgccggtccg gcgcagacct atggcacgac
      481 cgtccccgtc acacccacac ccgacggctc cgacaacaag ggcagtaaca gcgagttgtc
      541 gcctgagctg ccgccgccgg acttcccgac tgcgaacgtc gaggggtaca ccttcgacct
      601 gcgctatgca ctgagcgcgg aactggacaa ggtgccgcgc gaggctgcgg tctacgagct
      661 gctccgggac gagccgacgg tcgcgggcgt gcagaagatt gccgataagc tcggcattga
      721 tggcaaggcg gaagaccggg gcgatggctc cttcacggcg tctgggaacg gcgaactgtt
      781 cgtggccatc gatcaggtca actacttctc ggacgtggac gttgcgcagg gcgaccttcc
      841 ctccgatgac aaggcgatca acgaggcccg ggattggctc cgccacgcgt cgctcctccc
      901 gcccgacatc ggcaagggct cgattgttgg tcggttcccc gattccaatc ggatggttgt
      961 gagcttcgcg ccggccgagc cggagaacat cctctccggg tatccgagta tcggtctcac
     1021 gatcggcgcg gatggcgcgg tgattgaggc atcgatccgc tgggcgaaca tcgtccgggc
     1081 ggacgtctat cagcttatgt cgactgatca ggcgtggcag ctcgtctcca gcgggcaggc
     1141 ctacctcgcg ccggacctga cgcaggcgaa gatcgacccc ggaaccgacg tcaagggccg
     1201 ggtaacattc acctcaatca agatcgccta cgcgacagcc ggtcctccgg gtggccgcca
     1261 gttcctccag ccgatctacg tcttcgaagg acgaatccgg ccggagggcg acaagcggac
     1321 ctacccgatg accgcctacg tgtcggcgct ctcgaactcc ggcgctccgg tcggctgatc
     1381 gccgcacaat caggaacggg tgaatggcaa agcggatcct cgtcctcaac ggtccgaacc
     1441 tgaacatgct gggcacgcgc cagcctgaga tatatggtcg gacgtctctc gctgatatcg
     1501 aggtcgccat gcggagcgtg gcgcaatcgg tccggtcgga tgtggagctt tcgttttttc
     1561 agtccaacca cgaaggcgcg ttaatcgatg tcatccagca atcggccggc aaggtcgatg
     1621 ccgtgatcat taatcccggc ggactgaccc ataccagcgt cgcgctgcgg gacgcgctgg
     1681 tcgcgctcga ccggccggtg gtggaggtcc acctcaccaa catccacgcc cgggagccat
     1741 tccggcatca ctcgtacatc gccccgatcg cgatcgggca gattgctgga ctcggcgcgg
     1801 cgggatactt actggcggtg cggtacgtgg tcgagacggt gctggcatga gcagcaatcg
     1861 catcgaacag gttcgggcca cccttgcgga gctcggcacg ggggccctga ttctcacgag
     1921 tcccgcaaac cggcgctggg cgacgggctt cacgtccgcg gccggcgttc cgttttcgac
     1981 ggacctcgcg atcatcacac cgtccgcggt cgagttgctt gtcgcgccga tccacgtcgg
     2041 ttgggcgcgc ggcgaatcgg gcgtcgccac cgatgtccgc gcgcacaagg gccagttcgc
     2101 atcgagtttt gcggattatc tgagcgagcg cgggttaacg cacatctcac tggaggcgga
     2161 cgccctcccc taccccgagg ccctcaagat ccaggacgcc acgcccgtgg tggcgatcga
     2221 atttgtgact ggactgcgcg atacgtggcg agcgtcgaag gatccggacg aaatagcgtc
     2281 gctggagcgg gccgctcgca tcacggacga ggtcttcatc gaaatgtccg gctggatcgc
     2341 gccgggcatg gacgagacga gcgtcgcccg ccggatttcg tcgcagctga tcgagcgcag
     2401 cgacggcctc gccttcgacg tgatcgtggc ga
//
LOCUS       DASSGX010000494         3652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_522224, whole genome shotgun sequence.
ACCESSION   DASSGX010000494 DASSGX010000000
VERSION     DASSGX010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522224"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VFI12_08830"
     CDS             <1..224
                     /locus_tag="VFI12_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056554.1"
                     /translation="YCSDGDRCAADCHCNQGGTDGDRCAANCYCDQGCANGDRCPADF
                     DSHAGSADCHRGSADRDSYCRAATAEGPE"
     gene            301..513
                     /locus_tag="VFI12_08835"
     CDS             301..513
                     /locus_tag="VFI12_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056555.1"
                     /translation="MRRSENGGGWVTIADGLQANTASYQDRNVNGGSTYTYVVEVYNP
                     GGSADSNIATVGGSSIGNKGNSGKSK"
     gene            927..1283
                     /locus_tag="VFI12_08840"
     CDS             927..1283
                     /locus_tag="VFI12_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056556.1"
                     /translation="MIAATMRLTMRDTSAAGYCGSCFDDAFSTKRLDGDCYSCKVPES
                     GCVATRSGVQADDLSPDVAGGLFCRCQGRNCIVNGITFEKNADVTAGTSGACRPYLIT
                     GTRYDLCIGPAQSLPA"
     gene            1297..2190
                     /locus_tag="VFI12_08845"
     CDS             1297..2190
                     /locus_tag="VFI12_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056557.1"
                     /translation="MSGRPLMILERDGVELGEIVPAAPLRRISLHGLTAAVQGPRILQ
                     RTVELVLRAYPAPEIGSGDDLTISANRGTGSRKSWIISSENFTQRAYSGVQEAARWVE
                     WVFISEALKRWDQFIHVHAGLVANSTQSALLIGRSGSGKSTTTTALALAGMQLYSDDV
                     ALVHREGLRPASAPRPIKLDPGARRLLRAHGLRLPRGSWLGESIDRTVIPGLPPVEEP
                     GPPLTTAIFFADQRLSTPVLRRLTSAEAVMRLVQQSVSETFDASGPSSGAVNLINAIQ
                     CFELAAGDLGSTVSVIQNLLA"
     gene            2327..>3652
                     /locus_tag="VFI12_08850"
     CDS             2327..>3652
                     /locus_tag="VFI12_08850"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HET7056558.1"
                     /translation="MDTILLLARLFLAGVFTLSGITKLADPDGSRRAVAGFGVPERYA
                     LPAGIALPIVELALAVLLLPVATAWWGALGALLLLAGFIAGIAYNLRQGKTPDCHCFG
                     KIHSEPIGAPTLIRDGAFAAVAAFIVLFGWGDAGTSTIGWFRDLTNFERVLTVTSVLL
                     IAAVAGLAWLLLQMVRQNGRLLIRLDSIDNRLAGADVPVTDAASAPESVTQPGLPIGT
                     PAPAFRLDGIYGEAQTLDSLRAAGNPVVLIFSSPTCGPCTALMPEVGRWQREYADQLT
                     IAMIGQGEPAANRAKSAEHGLTNVLLQQSNEVAQLYDAKGTPTAVLISADGTIGSAVA
                     PGAEAIRALVTQAAASTPKRAPVAAGRTVENQPPSGNGIVQVVEEIPVQEQEIDDSHE
                     PAPQINLPDLDGNMVSLADFAGDPTVVLFWNPGCGFCKRMLDDLKTWEAN"
BASE COUNT          665 a         1214 c         1112 g          661 t
ORIGIN      
        1 actactgttc cgacggcgac cgctgtgccg ccgactgcca ctgcaaccaa ggcggcaccg
       61 acggcgaccg ctgtgcagcc aactgctact gcgaccaagg ctgcgccaac ggcgaccgct
      121 gtcccgccga cttcgactcc catgccggct cagccgactg ccaccgcggt tccgccgacc
      181 gcgacagcta ctgccgcgcc gcaaccgccg aaggccccga gtaatctcaa tggccggagc
      241 aagcgcggcg cgaatcagct cacctggtcc gataacgcca cgaacgaaac cggataccgg
      301 gtgcgccgca gcgagaacgg cggcggttgg gtgaccatcg ccgacggctt gcaggcaaac
      361 accgcctcct accaggaccg caacgtcaac ggcggcagca catacaccta tgttgttgag
      421 gtctacaacc ccggcggcag tgctgactcc aacattgcaa ctgtcggagg gagctcgatc
      481 ggaaacaagg gcaattccgg caagagcaag tagcccgtct cagcggggac ccaagtcggg
      541 tccccgctct tttattgtcc cgactttcgt tctgtcttcc gttctggccg gcctgaatgc
      601 gactcgacca ccggggtgca gcgactccag ccggacagat cctggtcgga cttttacgct
      661 tgcgcgtaat caggcgaaaa atcgggacct gtacattcaa ttgcctccga taactaactc
      721 cactacaatg acccgaacgg gccaggacat tcgggtggcc agcccaatca gagcattcgg
      781 caaatcccgg taagcgagtc gcgtgtgctc caaaaatgcc ggtgaatagc acaacgcaga
      841 ggtccgagtt gaatcaagac actcagcatc gcgtctcgcg acgcaccgca gttgcctcgg
      901 gcgtccgtct tgcctacgga gcgccgatga tcgcggccac catgcggctg acgatgcgag
      961 acacctcggc agccggctat tgcggctcgt gcttcgatga cgcgttcagc acgaagcggc
     1021 tcgatggaga ttgctactcc tgcaaggtcc cggagtccgg ctgtgtggca acacgcagtg
     1081 gggtgcaggc ggatgatctt agccccgatg tcgccggagg gttgttctgc cggtgccagg
     1141 gtagaaactg catcgtcaac ggcatcacgt tcgagaagaa cgcggatgtg accgccggca
     1201 caagcggcgc ctgccggccg tacctgatta ccggcacccg atacgatctg tgcatcggcc
     1261 cggcccaaag cctgccggct tgacaatcat ctgatcatga gtggacggcc gctgatgatt
     1321 ctggaacgtg atggtgtgga gctgggcgag atcgtgccag cagcaccgct ccggcggata
     1381 tccctgcacg gactcaccgc ggcggtacaa ggtccgcgaa tcctgcaacg aacggtcgag
     1441 ctcgtccttc gcgcgtaccc agcgcctgag attggtagcg gcgatgatct gacaatctcg
     1501 gcaaaccggg gaaccggatc gcggaagtcc tggatcattt cgtctgaaaa cttcacccag
     1561 cgcgcgtaca gtggcgtgca ggaagccgcg cgatgggtgg aatgggtctt tatctccgag
     1621 gcattgaagc gctgggacca gttcattcac gtccacgccg gtctcgtcgc caactcaacc
     1681 cagagcgcgc tcctgatcgg ccgctcgggc agcggcaaga gcaccacaac caccgcgctc
     1741 gcccttgccg gaatgcagct ctactccgac gatgtcgcgc tcgtgcatcg cgaggggttg
     1801 cgcccggcct ccgcgccgcg cccgatcaaa ctcgaccccg gtgctcgccg cctgctccga
     1861 gcgcacggtc tccgtcttcc ccggggatca tggctcggcg agagcatcga tcggaccgtc
     1921 atcccgggtt tgccccccgt tgaggagccc ggcccacccc ttacgaccgc catcttcttc
     1981 gcagaccaac ggctttcgac accggtcctc cggcgactga caagcgccga ggctgtcatg
     2041 cggctcgtcc agcaatctgt ttccgagacg ttcgacgcgt cggggccgtc atcgggagct
     2101 gtgaacctga tcaacgctat tcaatgcttt gaattagccg ccggagatct cggttccacg
     2161 gtttcagtta tccaaaatct gctcgcctga tcacggattt gccactcggc accggttgac
     2221 cagatcacta caataggtaa agttatagac actgcagtct cggaatccgc atcggccctg
     2281 gcgcccctcg ccctcgccac ggctcgttcg tgaaggtact ccgcgcatgg acacgatcct
     2341 cctcctcgcc cgtctcttcc ttgccggcgt cttcaccctc tccggaatca caaaactcgc
     2401 cgatccggac ggatcgcgcc gggcagtggc gggcttcggt gtgccggagc ggtatgccct
     2461 cccggcgggc atcgctttgc cgatcgtgga attggcgctg gcggtgctgc tcctgccggt
     2521 cgcgacggcg tggtggggtg cgctcggcgc cctgttgctc ctggccggct tcatcgcggg
     2581 catcgcctat aacctgcgcc agggcaagac gccggactgt cattgcttcg ggaaaatcca
     2641 ttccgagccg atcggcgcgc cgacgctgat ccgggatggc gcgtttgccg ctgtcgccgc
     2701 attcatcgtc ctcttcggct ggggcgacgc cggcacgagc acgatcggct ggttccggga
     2761 ccttacgaac ttcgaacggg tcctgaccgt cacgtcggtt ctgctgatcg cggcggtcgc
     2821 aggcctggcg tggttgcttc tgcaaatggt gcggcaaaac gggcgcctcc tgatccggct
     2881 cgactcgatc gataatcgtc tcgctggtgc cgatgttccc gttaccgacg cggcctccgc
     2941 gcccgagtcc gtgacgcagc cgggccttcc gatcggcaca ccggcgcccg cattccgcct
     3001 cgacgggatc tatggcgagg cgcagactct tgattcgctt cgagctgccg ggaaccccgt
     3061 cgttctgatc ttctcgtcgc cgacatgtgg gccgtgcact gcgttgatgc ccgaggtcgg
     3121 gcggtggcag cgcgaatacg cggaccaact gactatcgcg atgatcggcc agggcgagcc
     3181 agccgccaac cgcgccaaga gcgccgagca cgggcttacc aacgtcctgc tgcaacagtc
     3241 gaacgaagta gcgcagcttt atgacgcgaa gggcacgccg actgccgtcc tgatatccgc
     3301 ggacggcacg atcgggtcgg ccgttgcccc gggtgcggag gcaatccgcg cgctcgtgac
     3361 gcaggccgcc gcgagcacac ccaagcgagc tccggtcgcc gccgggcgca cggtcgagaa
     3421 tcagcccccg tccggcaacg gaatcgtcca ggtggtagag gagattccgg tgcaggaaca
     3481 agaaattgat gacagtcacg aaccggcgcc ccagatcaac cttccggatc tggacggaaa
     3541 tatggtgtcg ctggctgatt tcgcaggcga tccgaccgtc gtccttttct ggaacccggg
     3601 ctgcggcttc tgcaagcgca tgctggatga tctcaagacc tgggaagcga ac
//
LOCUS       DASSGX010000495         3087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_961005, whole genome shotgun sequence.
ACCESSION   DASSGX010000495 DASSGX010000000
VERSION     DASSGX010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961005"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..1444)
                     /gene="sufB"
                     /locus_tag="VFI12_08855"
     CDS             complement(38..1444)
                     /gene="sufB"
                     /locus_tag="VFI12_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HET7056559.1"
                     /translation="MVATPKPQVDIGEYQYGFRDEEDYFFKSKRGLDRETVLEISKMK
                     GEPDWMLEFRLKAYDHFMKRPMPNWGGDLSGINFDDIFYYIRPSEKQGKTWDEVPEYI
                     KDTFERLGIPQAERKYLAGVGAQYESEVVYHSLREDLQKQGVIFLDMDAAVREHPDLV
                     KQYFGTIIPANDNKFAALNSAVWSGGSFIYVPEGVRVEVPLQAYFRINAENMGQFERT
                     LIICAPNSYVHYVEGCTAPTYSSDSLHCAVVEIIVQEGARCRYTTIQNWSKNVYNLVT
                     KRAAAYKNATMEWVDGNLGSKLTMKYPAVWLMEEGARGEILSVAFSGDGQHQDAGGKV
                     VHVAPHTSSQIISKSISKGDGRSSYRGLEKVHKGARDVKSNVVCDALLLDETSKTDTY
                     PYIEIEEEEVSVGHEATVSKVAEEQIFYLQSRGLSEDEAMSMIVNGFIEPIAKELPLE
                     YAVELNRLIQLEMEGSVG"
     gene            complement(1466..2263)
                     /gene="sufC"
                     /locus_tag="VFI12_08860"
     CDS             complement(1466..2263)
                     /gene="sufC"
                     /locus_tag="VFI12_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HET7056560.1"
                     /translation="MSTPMFEIEDLHVSIEDKEILKGVNLTVNKGEVHALMGPNGSGK
                     STLANVIAGHPAYEVTEGSIRYNGEDILEWEPDERARKGLFLAFQYPTVIPGVSMANF
                     LRMAVTNVRNARAEDAAATGGSVAKLAPREFRKLMREKMSLLKMDDQFATRYLNEGFS
                     GGEKKRAEILQMALLEPTLAVLDETDSGLDIDALRVVSEGVNALAGPEMGILLITHYQ
                     RLLNHIKPDFVHIMMEGRIVKEGGPELAHELEAKGYETLRAEFSVAV"
     gene            complement(2316..2759)
                     /locus_tag="VFI12_08865"
     CDS             complement(2316..2759)
                     /locus_tag="VFI12_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HET7056561.1"
                     /translation="MADIVRDSKSNPPAQAFRPTAQRLLVLDVVSKADRHLTAAEIFE
                     RVRQTDPRIGYGTVYRSLHLLVEHGLIQELTFADQASRYDGRTDRHDHVHCNICGALF
                     DVEVPVALMARHVAEERSGFSITSHHTVFSGECPCCRTGSRPHTS"
BASE COUNT          551 a          958 c          958 g          620 t
ORIGIN      
        1 cggagcgatg gcggaatgcc atcgctccgc gggctgacta gccgaccgag ccttccattt
       61 cgagctggat cagccggttc aactcaacgg cgtactccag cgggagctcc ttcgcaatcg
      121 gctcgataaa gccgttcacg atcatgctca tcgcctcgtc ctcgctcagg ccgcggctct
      181 gcagatagaa gatctgctcc tccgccacct tggagacggt cgcctcgtgc ccgaccgaga
      241 cttcctcttc ctcgatctcg atgtacgggt acgtatcggt cttgctggtc tcgtcgagca
      301 ggagtgcgtc gcagacgacg ttactcttca cgtcgcgcgc gcccttgtgc accttctcca
      361 gaccgcgata gctggaccgg ccgtcaccct tcgagatcga cttcgagatg atctgcgacg
      421 acgtatgcgg agcaacgtgg acgaccttgc cgccggcatc ctggtgctgg ccgtcacccg
      481 agaaggcgac cgagaggatc tcgccacggg cgccctcctc catcagccag accgccgggt
      541 acttcatggt caacttggag ccgagattac cgtcgaccca ctccatcgtc gcgttcttgt
      601 aggccgcggc ccgcttggtg acgaggttgt agacgttctt cgaccagttc tggatcgtcg
      661 tgtagcggca gcgcgcccct tcctggacga tgatctcgac caccgcgcag tgcagcgagt
      721 cggaggaata ggtcggggcc gtgcagccct caacgtagtg gacgtagctg ttcggggcgc
      781 agatgatcag cgtccgctcg aactggccca tgttttcggc gttgatccgg aaataggcct
      841 ggagcggtac ttcgacccgg acgccttccg gcacgtagat gaaggaaccg ccggaccaga
      901 cggccgagtt cagcgcggca aacttgttgt cattcgccgg gatgatcgtg ccgaaatact
      961 gcttgaccag gtccggatgc tcgcgaacgg ccgcatccat atccaggaag atcacgccct
     1021 gcttctggag atcctcacgc agcgagtggt agaccacttc cgattcgtac tgcgcgccga
     1081 cgccggccag gtacttgcgc tccgcctggg ggatgcccag tcgctcgaag gtgtccttga
     1141 tgtattccgg cacctcgtcc caggtcttgc cctgcttctc ggacggccgg atgtaataga
     1201 agatgtcgtc gaaattgatc ccgctgaggt cgccgcccca gttcggcatc ggccgcttca
     1261 tgaagtggtc gtatgccttg aggcggaact cgagcatcca gtcgggctcg cccttcatct
     1321 tcgagatttc gagcaccgtt tcgcggtcaa gaccgcgctt gctcttgaag aagtagtctt
     1381 cctcgtctcg aaaaccgtac tggtactcgc cgatatcgac ttgcggcttt ggtgttgcaa
     1441 ccatgtatcg ctccttgctg tcgggttaga ccgcgacaga gaactcggcg cggagggtct
     1501 cgtacccctt cgcctcgagc tcgtgggcca gctcaggacc gccttccttg acgatccggc
     1561 cttccatcat gatgtggacg aaatccggct tgatgtgatt cagcagccgc tggtagtggg
     1621 tgatcaggag aatgcccatc tccgggcccg caagcgcgtt cacgccctcg gagacgacgc
     1681 ggagcgcgtc gatgtcgagg ccggaatccg tctcgtcaag gacagccagt gtgggctcca
     1741 gcagggccat ctgcaggatc tcggcgcgct tcttctcgcc cccggagaag ccctcgttca
     1801 gatagcgggt cgcgaactgg tcgtccatct tcaggagcga catcttctcg cgcatcagct
     1861 tgcggaactc gcgcggagcc agcttcgcga cgctgccacc ggtggcggcc gcatcttccg
     1921 cccgcgcatt gcggacattg gtgacggcca tccgcaggaa gttcgccatc gagacgcccg
     1981 ggatcacggt cgggtactgg aaggccagga acaagccctt gcgcgcccgc tcgtccggct
     2041 cccactcgag gatgtcctcg ccgttgtacc ggatgctgcc ttccgtgact tcgtaggccg
     2101 ggtgaccggc gatcacgttg gccagggtgc tcttgccgga gccgttcggg cccatcagcg
     2161 catgaacctc gcccttgttg acggtcaggt tgacgccctt gaggatctct ttatcctcaa
     2221 tcgagacgtg cagatcttcg atctcaaaca tcggggtgct catcgaacag actgtccctc
     2281 ctgatgggtg gttgtgcgtc caggttgacg caatctcacg atgtgtgtgg ccgggatccc
     2341 gtgcggcagc acgggcactc tcccgaaaag acggtgtggt gactggtgat ggagaatccg
     2401 ctgcgctcct cggcgacatg gcgggccatc agcgcgaccg gcacctcgac atcgaacagc
     2461 gccccgcaga tattgcagtg cacgtggtca tggcgatcgg tgcggccgtc gtagcggctg
     2521 gcctggtcgg cgaaggtaag ctcctggatc agcccatgct caaccagcag gtgcagggag
     2581 cgatagaccg tgccgtaccc gattcgcgga tccgtctgcc gcacgcgctc gaagatctcg
     2641 gcggcggtca gatgccggtc ggccttcgag acgacatcga gcaccagcaa acgctgggcc
     2701 gtcggtcgaa acgcctgagc cggcgggttg gatttggaat cgcgaacgat atcagccatc
     2761 tcactccagg cgaacgaggc acaacttgag aatgagtctc gttcccggag attaattcta
     2821 gtagattggt cagattaaat ctagtctagg aggcgcgaag gcgcggaggc gcgaaggcgc
     2881 ggaggcgcgg aggcgcggag gcgcgacgat tgtaggggtt tgcgggggca aacccgcgcc
     2941 gcttcccgat aggttcggtc acatgtgagc gttcgtgccc gggaccgccc ggccgtaaac
     3001 acccgggctg aatccaataa gcccgctaaa gcaggctgga gaacacgctt cgaagttgga
     3061 cgtatcggga tccggtagat ttcaagc
//
LOCUS       DASSGX010000496         3926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1067138, whole genome shotgun sequence.
ACCESSION   DASSGX010000496 DASSGX010000000
VERSION     DASSGX010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1067138"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1704
                     /locus_tag="VFI12_08870"
     CDS             <1..1704
                     /locus_tag="VFI12_08870"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HET7056562.1"
                     /translation="ERPELAETCGAAGITFVGPSAATLEICGDKLATREAAVRAGVPV
                     LPASEPIGDTPESWIAAGRMIGYPLLVKPAEAGGGRGLRFVVDESGLVEAIQASRRES
                     ASSGAGAIIYLERAMIEPRHVEVQVAADSNGVLVVGDRDCSLQRRHQKVIEEAPAPNI
                     DGDTRAALHESARRVAIEVGLRGIATCEFLLGANGDLAFLEVNPRIQVEHPVTELVTG
                     IDLVAWQLLIASGQELPIRSTPPPRGHAVEARVYAEDPFAGFMPTVGKLATVSWPTGP
                     GIRVDAGYASGDTIPTAYDAMLAKVCAVGSDRTSALMTLRNALTDTMIAGVRTNVPWL
                     LNLLADEAVKSGRATTATVAAITPTMPERSIAPVAAVAYLLERSASGSSDAWSAIGPW
                     RMFGPAMITVHGEDWEEQIAACRSHLGWEARFGPVQIPVRWWTAPDGIITLSSPDTVM
                     KVAVVDRDGALEIFGNGGRWLVQIGPRPTVTTTRQRRPGNGSVRSPLPATVLGVHVAA
                     GDHVSHGQPLVTLSAMKMELVCDAPADGVVERITCRVGELVDADQELVRLRLANSETQ
                     S"
     gene            complement(1711..2076)
                     /locus_tag="VFI12_08875"
     CDS             complement(1711..2076)
                     /locus_tag="VFI12_08875"
                     /inference="COORDINATES: protein motif:HMM:NF017690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7056563.1"
                     /translation="MDVTKIGGYSPDMALDDYGPISKSIGTALDGPMNHRGKVFWSKS
                     DGSVTCGLWEVDAGRFSCAFDGEGEMVHVVKGTIIATTDSGEVVELGEGAVYTFTPGW
                     TGIWEMPTPMRKFFTTFTE"
     gene            2194..2847
                     /gene="msrA"
                     /locus_tag="VFI12_08880"
     CDS             2194..2847
                     /gene="msrA"
                     /locus_tag="VFI12_08880"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HET7056564.1"
                     /translation="MLLFKSDKTKMVSPEEALPGRDRPSFAIPTNHEVLGTPIQGPFP
                     EGIETAVFGLGCFWGAERLFWKLPGVYTTASGYSGGFTPYPTYEEVCTGKTGHAEVVL
                     VAYDPKRVSYDALLKTYFEAHDPTQGMRQQNDIGTQYRSTIYVTPEQEETARRVAAEY
                     EKALTAKGLGPLTTEIKPIGPFYYAEDYHQQYLHKVPSGYCGLKGTGVSCAIDYSAK"
     gene            complement(2934..>3926)
                     /locus_tag="VFI12_08885"
     CDS             complement(2934..>3926)
                     /locus_tag="VFI12_08885"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HET7056565.1"
                     /translation="LAVCLVLLSVLGVFIGPAPASAKDPIPVVIDSDMTTDDWMATLF
                     LLNDPDFDVKAITVTGTGFAYCDAGVQSALGVLALADYGDVPVSCGSESPLLGDNAPP
                     AEWRTTSEMVSELGLPEGGTPAGQDAVALFTSVIQDSPVPVTVLALGPLTNVAAAFEA
                     SPTLVKKVEMIYVMGGAIDVEGSHVSDANTAAEWNIYCDPYAARLVFESGAPITLIPL
                     DATNDVPVTQEFVAQLETQKVTPEAEFVATLLANNAESIASGRYYFWDPLAAVVMSDP
                     SMVTLTPRDVTVVDIPGAPDDGRTKPVANGSEILVATAPNGEALEAALLAGWNS"
BASE COUNT          753 a         1254 c         1219 g          700 t
ORIGIN      
        1 gagcggccgg aactggccga gacatgcggg gcggccggca ttacgtttgt cggcccgtcg
       61 gccgcgacac tggagatctg cggggacaag ttggcgacgc gcgaggccgc cgtacgtgct
      121 ggcgtgccgg tcttgccggc cagcgaaccg atcggcgaca cgcccgaatc atggatagcg
      181 gccggtcgta tgatcggata tccgcttctc gtcaagccgg ccgaggcagg tggcgggcga
      241 ggactccggt tcgtcgttga cgaatccggt ctcgttgagg caatccaggc gtcgcgccgg
      301 gaaagcgcgt cctcaggcgc cggcgcgatc atttacctcg agcgcgcgat gatcgagccg
      361 cgccacgttg aagtccaggt ggccgcggat tcgaatggcg tgctggtcgt cggcgaccgg
      421 gattgctcgc tgcagcgccg ccaccaaaaa gtgatcgagg aggcgcccgc accgaatatc
      481 gacggcgata cacgggccgc gcttcacgaa tcggcccgtc gagtggccat tgaagtcggc
      541 ttgcgcggga tcgccacctg cgagttcttg ctaggagcaa acggcgatct cgcgttcctg
      601 gaggtcaatc cgcgcatcca ggtcgaacac ccggtcacgg agctggttac tggaatcgat
      661 ctggtcgcgt ggcagttgct gatcgcatcc ggccaggaac ttccgatccg ctcaacaccc
      721 ccaccccgag ggcatgcggt cgaggcgcgg gtctacgcgg aggatccttt tgccggcttc
      781 atgccgacag tcggcaaact ggcaaccgtc tcgtggccga ccggtcccgg catccgggtc
      841 gatgccgggt acgcgagtgg tgacacgata ccgaccgcct acgacgcgat gctggccaag
      901 gtttgcgccg ttggatcgga tcgaacatcc gcactcatga cactgcgaaa cgccttgacg
      961 gataccatga tcgccggcgt ccgcacgaac gttccctggc tcctcaacct gcttgccgat
     1021 gaggcggtga aatccggtcg agccaccacg gcgacggttg ccgctatcac gccaactatg
     1081 cctgaacgct ccatagcgcc agtcgcggcg gtcgcctacc tcctcgagcg ctctgcgtcg
     1141 ggctcgtccg acgcatggtc ggcgatcggc ccatggcgca tgttcggacc cgcgatgatt
     1201 actgtccacg gcgaagattg ggaagagcaa atcgccgctt gccgatcaca tttgggctgg
     1261 gaagcccgat tcggtcccgt acagatcccg gttcgctggt ggacagcgcc ggacgggatc
     1321 atcaccctct cgtcacccga cacagtgatg aaggtggccg tggtcgaccg agacggcgcg
     1381 ctggaaatat tcgggaatgg cggccgctgg ctggttcaga tcggaccgcg accgacggta
     1441 accactaccc ggcagcgccg cccgggcaac ggaagcgtac gatccccgct cccagccacc
     1501 gtgctcggcg tccatgttgc ggccggcgac catgtctccc atggtcagcc gctcgtcacc
     1561 ctctcggcaa tgaagatgga gttggtctgc gacgcgcccg ccgacggcgt ggtggagcgc
     1621 atcacctgtc gcgtcggcga actcgtcgat gccgaccagg agcttgtcag gcttcgcctc
     1681 gcaaactccg aaacacagtc ctgagttgcc ctactcggta aaggtcgtga agaatttgcg
     1741 catcggcgtc ggcatttccc aaatgcctgt ccagccaggc gtgaaggtat agacggcgcc
     1801 ctcacctaac tcaacgacct cgccgctgtc agtggtcgca attatcgtgc ccttgacgac
     1861 gtggaccatc tcgccctcgc catcgaacgc gcaggagaac cgacccgcgt ccacctccca
     1921 caggccacac gtcacgctgc cgtcggactt cgaccagaag accttgccgc ggtgattcat
     1981 cggtccatcg agcgcagtgc cgatgctctt gctgatcggc ccgtagtcgt ccagcgccat
     2041 gtcaggagaa tagccgccga ttttagtcac atccaccggt tccgtccctt cgttaatata
     2101 gtgaggcgtg tgcccatgcc ttcttgtgca actcccggta ccatggagat agtctacggt
     2161 cgtaatcgat agttgattac gcaaggagtc ataatgcttc tcttcaagtc agacaagaca
     2221 aagatggtca gcccggagga ggccctccct ggccgcgaca ggccatcgtt cgcaatcccg
     2281 acgaaccacg aagtcctcgg cacgccgatc cagggtccat ttccggaggg gatcgaaacc
     2341 gcggtcttcg gactcggctg cttctggggc gctgagcggc tattctggaa gctgccgggc
     2401 gtctacacga ccgcctcggg ctactccgga ggcttcacac catacccgac atatgaggaa
     2461 gtctgcacgg gcaagaccgg gcatgccgaa gtggtattgg tcgcctacga tccgaagcgc
     2521 gtctcgtatg acgcgctgtt gaagacctac ttcgaagcgc acgatccaac gcagggcatg
     2581 cgccagcaga acgacattgg cacccagtac cgctcgacga tttacgtaac ccccgagcag
     2641 gaggaaacgg cccgccgggt cgcggccgaa tacgagaagg ccttgacggc gaagggcctc
     2701 ggcccgctca cgaccgagat caaaccgatc ggtccgttct actacgcgga ggactatcac
     2761 cagcagtacc tgcacaaagt gccgagtggc tactgcggcc tgaagggcac cggcgtaagc
     2821 tgcgcgatcg actattccgc gaagtagtcc cggccggccc ggaacttacg aagcgcgagt
     2881 ttcaatggag ggggatgcgg tgtacaccgc atccccctcc gcttgttttt acctcacgag
     2941 ttccagccgg cgagcagcgc ggcttcgagg gcttcgccgt tcggcgccgt ggcgacgaga
     3001 atctccgaac cgttggcgac gggcttggtg cgtccgtcgt caggcgcgcc ggggatgtcg
     3061 accacagtca cgtcgcgggg agtgagggtc accatactcg gatcgctcat cacgaccgcc
     3121 gcgagcggat cccagaagta atagcggccc gatgcgatcg actcggcatt gttggcaagc
     3181 aaagtggcga cgaactcagc ctcaggcgtc accttctgcg tttccagttg ggcgacaaac
     3241 tcttgtgtga ccggcacgtc gttggtcgca tcgagcggga tgagcgtgat cggcgccccg
     3301 gattcgaaga cgaggcgcgc tgcgtacgga tcgcagtaga tgttccactc agcggcggtg
     3361 ttcgcgtcgc tgacatggga accctctacg tcgatcgctc cgcccatcac gtagatcatc
     3421 tcgaccttct tgaccaacgt tggtgatgcc tcgaaggctg ccgcgacgtt tgtcaatggc
     3481 cccaacgcga ggacggtcac cggaaccgga gaatcctgaa taactgatgt gaagagcgcg
     3541 acggcatcct ggccggcggg cgttccaccc tctggaaggc caagttcact gaccatctcc
     3601 gaggtcgtgc gccactccgc aggcggcgcg ttatcgccca gcagaggcga ttccgatccg
     3661 cagctcaccg gcacgtcgcc gtaatccgcc agggcaagca cgccaagggc ggattggaca
     3721 ccggcatcgc aatacgcaaa gcccgtaccg gtaaccgtga tggctttgac gtcgaaatcg
     3781 ggatcattca gcagaaacag ggtcgccatc cagtcgtccg tggtcatgtc gctgtcgata
     3841 accacgggaa tggggtcctt ggccgacgcg ggtgccggtc cgatgaaaac gccaagcaca
     3901 ctgagcagca ccaggcaaac cgccaa
//
LOCUS       DASSGX010000497         3126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_741444, whole genome shotgun sequence.
ACCESSION   DASSGX010000497 DASSGX010000000
VERSION     DASSGX010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741444"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..2284)
                     /locus_tag="VFI12_08890"
     CDS             complement(20..2284)
                     /locus_tag="VFI12_08890"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET7056566.1"
                     /translation="MTRDERFFPDDAALDQQWDDLVQGHAGLTTEPVLRDLHTSRPPA
                     GPSPMFRAQLRAMIQTAAAEMPGATRTEPIRLMPGVPATAETARPQPVPVIARPRYSR
                     MRGLTGVSKAASVLLAASLLVAMLASVVRYPFGGSSPTDTPASGAQTAIPAASGSPES
                     AGSGPSSADPGRTNQQPGPAPMSIPQLIGRADVRGSAMALGGNTIVIVDNDTVTALDA
                     GTLKRKWAVTVPYGVYTAPVIAGDAVYFGFTGQRDGTTEWTLGPDKPNQLVSLSLEDG
                     SENWRIDGAGSAPVAPLVVNGTIFAAGTTTDDYRLTALDAADGHVLWTAGAFVSLKTG
                     GSPAVIPYLLMRLAYEDGLIAVNRLHGLSVYDAGTGKEIWTHAVDAPDSVDAPLIADG
                     TVVVTIGHQSGDADAPGSAKVVAFNLRTGQEQWAREPVTGNVFLNVSAAQRGDDIVWT
                     QFNAVNEWVASAASLVNGKQAWSVPSWDTAADPGPNFWQPRDSTVVAGDQLFTLGLVS
                     DRGNQARTLVTAREIATGDIAWSAIIDGSAKVAPIIAGGKVYVLTDLYGLQVLGGSES
                     TATPGAGTADLRGPDECAATPIEYPLLGTPEAEGTPIPEWARWGEIMPLSEVPAVDPA
                     KAAAPDVATAIENTLAAYRACMVQGDPQLLFGLFSTDYWQRLRGMEGWNFDGKNGERG
                     QTAVMLAGSNERIDLDPATLQSWPDGRVGGIVHGNTDMYVWFVEDGGTWRVDEFHHIM
                     DTSPIPEPSPSPEE"
     gene            complement(2281..2838)
                     /locus_tag="VFI12_08895"
     CDS             complement(2281..2838)
                     /locus_tag="VFI12_08895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7056567.1"
                     /translation="MTGPRPAELPDAHLVAMARRDPAAFVHLYDRYVDAIYRYCAQQL
                     SPTAAEDATSVAFLNALKAIEGFDPSRGGFRPWLYRIAHNAVIDQTRVRAHAPIDGFT
                     FEDPDDGPADRVVALDQRDTLLQAVRQLPPDQQQVIHLRLASLTGAEIAEAMGRSHVA
                     VRALQHRAIKRLRTLLQPIGKDDQI"
BASE COUNT          478 a         1035 c         1098 g          515 t
ORIGIN      
        1 cccgggtttg gtgtggctgc tactcctccg ggctggggga gggctcgggg atcggcgagg
       61 tgtccatgat gtggtggaac tcgtcgaccc tccacgtgcc gccgtcctcg acgaaccaga
      121 cgtacatgtc cgtgttgccg tggacgatgc cgccgacccg gccgtccggc caggactgga
      181 gcgtggccgg atcgaggtcg atccgctcgt tgctgccggc cagcatgact gcggtctgtc
      241 ccctctcgcc gtttttgccg tcgaagttcc agccttccat gccgcgcaac cgctgccagt
      301 agtcggtcga gaagagaccg aacaggagct gcgggtcgcc ctggaccatg caggcccggt
      361 aggcggccag cgtgttctcg atcgccgtgg cgacgtccgg tgcggcggcc ttggccgggt
      421 cgaccgcggg cacctcggag agcggcatga tctcgcccca ccgcgcccat tcggggatcg
      481 gcgtgccctc ggcctcgggc gtgccgagca gcgggtactc gatcggcgtc gccgcgcact
      541 cgtccgggcc gcgcaggtcg gccgtcccgg cccccggcgt ggcggtagat tctgacccgc
      601 cgagcacctg caggccgtag aggtcggtca ggacatacac cttaccgccg gcgatgattg
      661 gcgcgacctt cgcggagcca tcgatgattg cggaccaggc gatatcgccg gtagcgatct
      721 ccctcgcggt tacgagcgtt ctcgcctggt ttccccggtc cgaaaccagg ccgagcgtga
      781 acaactgatc gccggcgacg accgtcgagt cccgcggttg ccagaagttc ggaccgggat
      841 cagccgccgt atcccaactc ggcacagacc acgcctgctt accattcacc agactggccg
      901 cactggccac ccattcatta accgcgttga actgggtcca cacgatgtca tcgccgcgct
      961 gggcagcgct gacgttcaga aacacgttgc ccgtgaccgg ctcccgcgcc cactgttcct
     1021 gacccgtgcg gagattgaac gcgaccacct tggcggagcc gggcgcgtcg gcgtcgcccg
     1081 actggtgacc gatcgtcacg acgacggtcc catccgcgat gagcggcgca tcgacactgt
     1141 ccggagcatc aaccgcgtgc gtccagatct ccttcccggt tccagcgtcg tagaccgaga
     1201 ggccatgcag ccggttgacg gcaatcaacc catcctcgta cgcgagccgc atcaaaaggt
     1261 acgggatgac ggctggactg cccccggtct tcaggctgac gaaggccccc gccgtccaga
     1321 gcacgtgacc gtcggcggcg tcgagtgcgg tgagccggta gtcgtccgtt gtcgtgccgg
     1381 ccgcgaagat cgtgccgttg acgacaaggg gggcaaccgg agccgagccc gcgccgtcga
     1441 tccgccagtt ctcggagccg tcctcgagcg agagtgatac gagctggttc ggcttgtcag
     1501 gacccaacgt ccattcggtc gtgccatccc gttgcccggt gaacccgaag tagacggcgt
     1561 ccccggcgat gactggcgcg gtgtagacgc cgtagggcac cgtgaccgcc cacttccgct
     1621 tgagcgtgcc ggcgtcaagc gccgtgaccg tgtcattgtc gacaatcacg atggtgttgc
     1681 caccgagcgc catcgctgag ccgcggacat cggcgcggcc gatgagctgg gggatgctca
     1741 tcggggcggg gccgggttgc tggttcgtcc ggccgggatc ggcgctgctc ggcccgctgc
     1801 cggctgactc gggcgaaccg gatgcggcgg ggatcgccgt ctgcgcgccg gacgccgggg
     1861 tatcggtcgg ggatgagccg ccgaacgggt agcggaccac gctcgccagc atcgctacca
     1921 ggagcgacgc ggccagcagc acggatgcgg ccttcgagac tccggtcaga ccgcgcatcc
     1981 ggctgtatcg cggccgcgcg atcacgggga ccggctgcgg acgcgcggtt tcggccgttg
     2041 ccgggacccc gggcatcagg cggatcggct cagtccgggt cgcgccgggc atctcggcgg
     2101 cggcggtctg gatcatcgcc cggagctgcg cccggaacat cggcgacgga ccggccggcg
     2161 ggcggctggt atgcagatcg cgcagcaccg gctcggtcgt caacccggcg tggccctgaa
     2221 cgaggtcgtc ccattgctgg tcgagcgccg catcatcggg gaagaagcgt tcgtctctgg
     2281 tcatatctgg tcatccttgc caatcggctg aaggagcgtg cgaagtcgtt tgatggcgcg
     2341 gtgctgcaag gcgcggaccg caacgtggct gcggcccatc gcctcggcga tctccgcgcc
     2401 ggtcagcgat gcgagtcgga ggtggatcac ctgctgctgg tcgggcggca attgccggac
     2461 cgcctggagc agggtgtcgc gctggtcgag ggcgacaacg cgatccgccg ggccgtcgtc
     2521 cggatcctcg aatgtgaaac cgtcgattgg tgcgtgggcc cggacccggg tctgatcgat
     2581 cactgcgttg tgggcgatcc ggtagagcca gggccggaac ccgccgcggc tggggtcgaa
     2641 cccctcgatc gccttgagcg cgttcaggaa cgcgacgctg gtcgcatcct cggccgcggt
     2701 cgggctcagc tgctgcgcgc agtaccggta gatcgcgtcc acgtagcggt catagaggtg
     2761 tacgaaggcg gccggatcgc gccgcgccat cgcgacgagg tgcgcatccg gcaattcagc
     2821 cggtctcggt ccggtcatcc atgtatctgg ctcctccctg ggttcatgca ctggcaaacg
     2881 tccttgcaaa tcgaatcctc accgaggttg gccattcctc agacagtaca acggagcagg
     2941 ggcgaagggt gtgacggtgg tgaggcgaga aggcgaggag tcgagaagta ggacgcgaag
     3001 gcgcgacgtc atgtaggggc agatggcatc tgcccgcgct cctccgcacc gaccgcgtca
     3061 ggtgccacgg ccggtaacgg gtcgtcgcat gccaacgggc cggtagacga ggcccgggca
     3121 gatgcc
//
LOCUS       DASSGX010000498         2426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_196225, whole genome shotgun sequence.
ACCESSION   DASSGX010000498 DASSGX010000000
VERSION     DASSGX010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2426
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196225"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..971
                     /locus_tag="VFI12_08900"
     CDS             162..971
                     /locus_tag="VFI12_08900"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7056568.1"
                     /translation="MSAVAARTRRFSFGNLSLTAVAICVFIFLYIPLAVIVLYSFSGE
                     KVNVWPIGFYTTHWYSEMWNDPQIREGIMLSFRIGLIAAALAIVLGTMAAIAVDRFSF
                     PGKSMLRFCIVLPITLPGIVTGVAMLSFFSVVGLQLSNRTIILGHTTFCITLILNNVV
                     ARLSQLPRSLGEASADLGATPWRTFWRVTFPLILPAILSGGILAFTLSFDEIVVTLFL
                     RGRDATLPLVIWARLRRGFSPEINAAATMIIGISFVAVLLANRVSKWAGSS"
     gene            972..2066
                     /locus_tag="VFI12_08905"
     CDS             972..2066
                     /locus_tag="VFI12_08905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7056569.1"
                     /translation="MSMHVDGQGARIELISLEKRFNGVVAVRNIDMAVQPGEFLTMLG
                     PSGCGKTTTLRMIGGFEYPTGGQIMIDGQDVAGLQPHRRPVNTVFQQYALFPHMTVAR
                     NVGYGLEMAGVGKGERERRVVDALAMVRLANVERRKPSELSGGQQQRVALARALINRP
                     KALLLDEPLGALDLKLRKAMQLELKQLNRETGATFIYVTHDQEEALTMSDRIAVMNEG
                     RILQLGTPAEIYEHPVDRFVADFIGQSNLIPGVLEARNGETGAVRIATGGLVEARLRD
                     AAGIGDRVVVSIRPERITLDIGQDGGPGGAMDGVVSEIIYLGSHNQLVLHVMPDLRLA
                     ANVPTDFPAGPGDRASLHIPTRYATCFAAE"
     gene            2153..>2426
                     /locus_tag="VFI12_08910"
     CDS             2153..>2426
                     /locus_tag="VFI12_08910"
                     /inference="COORDINATES: protein motif:HMM:NF013577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056570.1"
                     /translation="MDKSARFRSVDCLTIIEQRLPALTGASRQVAEAIMRDPWAVLGM
                     TIYDVAEMSNVSLPSVTRFCRAVGFGGFRELVQGIAQSLGRIDSKDF"
BASE COUNT          398 a          776 c          776 g          476 t
ORIGIN      
        1 ggcgggccgg gcgaccagat gatcggcaag atcgtggccg accagttcgg cgtggccgac
       61 aattggccgc tcggcgccgc gctgatcatc cccgtgctgg cgctgatttc gctgctgctg
      121 atggtctcta accgaactgg cgcgatggag cgaacggcag tatgagcgcc gtagccgcca
      181 ggacccgtcg cttctcgttc ggcaacctct cactgaccgc cgtcgcgatt tgcgtcttca
      241 tcttcctcta catcccattg gcggtgatcg tcctctactc cttcagcggg gagaaggtga
      301 acgtctggcc gatcggcttc tacacgacgc actggtacag cgagatgtgg aacgatccgc
      361 agatccggga ggggatcatg ctctcattcc ggatcggcct gatcgccgcg gcgctcgcga
      421 tcgtgctcgg gacgatggcg gcgatcgcgg tcgatcgctt ctctttcccg ggcaaatcga
      481 tgctccggtt ctgcatcgtt ttgcccatca cgctgccggg catcgtgacc ggggtagcga
      541 tgctctcctt cttctcggtt gtcgggctgc agctctcaaa tcggacgatc atcctcggtc
      601 acaccacctt ctgcatcacg ctcatcctga acaacgtcgt ggcgcggctc tcgcagctgc
      661 cccgctcgct cggcgaggca tcggccgatc tcggggcgac gccatggcgg accttttggc
      721 gggtcacgtt tccgctgatc ctgcccgcca tcctttccgg cgggatcctc gcgttcacgc
      781 tatcgttcga cgagatcgtc gttacgctgt tcctcagggg gcgggacgcc acgctgccgc
      841 tcgtcatctg ggcgcgattg cggcgcggct tctcgccgga gatcaacgcc gccgcgacga
      901 tgatcatcgg catctcgttc gtggcggtgc tgcttgccaa tcgcgtttcc aaatgggctg
      961 gatcgtcctg aatgagcatg catgtcgacg ggcagggcgc ccggatcgaa ttgatcagcc
     1021 tcgagaagcg attcaatggt gttgtcgcgg tccgcaacat cgacatggcg gtgcagcctg
     1081 gcgagttcct gacgatgctc ggcccctccg gctgtggcaa gacgaccacg ctccgcatga
     1141 tcggcggctt cgaatacccg accggcggcc agatcatgat cgacggacag gacgtggccg
     1201 gcctgcagcc tcatcgccgt ccggtcaaca ccgtgttcca gcaatacgcg ctctttcccc
     1261 acatgacggt cgcgcggaat gtcgggtacg gcctcgagat ggccggcgtg ggcaagggtg
     1321 agcgggaacg gcgggtggtc gacgcgttgg ccatggtccg gcttgcgaat gtcgagcggc
     1381 gcaagccgtc cgagctgtca ggtggtcagc agcaacgggt tgccctggcg cgggcattga
     1441 tcaaccggcc gaaggcgctg ctcctcgatg agccgttggg cgcgctcgac ctcaagctgc
     1501 gcaaggcgat gcaactcgaa ctcaaacagc tcaaccggga gactggcgcg accttcatct
     1561 acgtcaccca cgaccaggaa gaggccctga ccatgtcgga ccggatcgcg gtcatgaacg
     1621 aggggcggat cctgcagctc ggcacgccgg ccgaaatcta cgagcacccg gttgaccggt
     1681 tcgtggccga tttcatcggt cagtccaacc tgatccccgg tgtcctcgag gcccgaaacg
     1741 gcgaaaccgg cgcggtgcgg atcgcgactg gcggcttggt cgaggccagg ttgcgggatg
     1801 ctgccgggat cggtgatcgc gtcgtcgtct cgatccggcc ggaacggatc acgcttgata
     1861 tcgggcagga cggggggcct ggcggcgcta tggacggcgt ggtgtccgaa atcatctacc
     1921 ttggtagcca caaccagctc gtgctccacg tgatgccgga cttgcgactc gccgccaatg
     1981 tgccgaccga tttcccggcg gggccaggtg atcgagcgtc cctgcatatc cccacgcgct
     2041 acgcaacctg cttcgcagcc gaataagtgc gcttgacggt ctgaaagcgg accgtagaat
     2101 acgcgaaagt ttctttcttc ccacgtaacc gcctttcggg tgaggtacag gcatggacaa
     2161 gtcggcccga tttcgctccg tcgattgcct cacgatcatt gagcagcgac tccctgcgct
     2221 gaccggagca tcccgccagg ttgccgaggc gatcatgcgc gacccatggg ccgtgctcgg
     2281 catgacgatc tacgacgtcg ccgagatgag caacgtcagc ctcccctccg tgacccgctt
     2341 ctgccgggcg gtcggattcg gaggcttccg ggagctggtg cagggcattg cgcagtcgct
     2401 tggacggatc gactccaagg atttcg
//
LOCUS       DASSGX010000499         2641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_741685, whole genome shotgun sequence.
ACCESSION   DASSGX010000499 DASSGX010000000
VERSION     DASSGX010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741685"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VFI12_08915"
     CDS             complement(<1..215)
                     /locus_tag="VFI12_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056571.1"
                     /translation="MAAPAAELTLTHRPSVLDRLPASALVMMAIFGFQLGSVIAKPAV
                     EDAGPVHTAFLRILFGAVILLAWRRPIV"
     gene            460..1386
                     /locus_tag="VFI12_08920"
     CDS             460..1386
                     /locus_tag="VFI12_08920"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HET7056572.1"
                     /translation="MKLAIIGVPFNSAGTHTAEALAPDALREAGLVTALARNNDVADY
                     GDVSFEPPRSEKRDRDSGLVAPHALVSMTGAVRDAAKRAYGEGRMPVVIGGDCAILLG
                     GLLAARDQLGYGPGLLFVDGHEDSYDPKKSLNGEAADMELGLATGFTKLTGLPELADE
                     LPIVDPHEIVLIGARDSAQVESEGHASIGTRVIVLDDTELRAAGVESTILRWLEQFQH
                     KPGRFWFHLDWDVLSADEMPAVSYPQPGGLAWDELETIARAALHADHLIGIDITLYNP
                     QLDPDGTAAGKIVAFLADVTGAPEREPRLGRI"
     gene            1422..2174
                     /locus_tag="VFI12_08925"
     CDS             1422..2174
                     /locus_tag="VFI12_08925"
                     /inference="COORDINATES: protein motif:HMM:NF018822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LppX_LprAFG lipoprotein"
                     /protein_id="HET7056573.1"
                     /translation="MLTRSTRRTFLQTTAAGLAAISIRPLRARADEDAAQLIAAAAKE
                     MAKVKTFHFDMETVDGKSTILENLELRKVVGDVVRPDSFQATLTAKVAVINVSVDVIS
                     VGGAVWVTDPLQSGKVWRQVAGDGEQNMGAAFTELINPDRLFLSAVSLIDDPKIDGTE
                     KVNGEDCTVVTGEFDPKRLSELASPVAGEEVATPSILTGEPVYLSAWINTGGRVLRIE
                     EEGPLTTSESNDVVRAITFSAFDEPVQITAPQ"
     gene            2207..2611
                     /locus_tag="VFI12_08930"
     CDS             2207..2611
                     /locus_tag="VFI12_08930"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7056574.1"
                     /translation="MQITRNSRQTMPGPKEWFTGDVFIDTVATPAGLSRAGAAMVHFT
                     PGARTAWHTHPRGQTIHVTEGIGRCQRRGGPIEVIQPGDSVYFEPGEEHWHGAAPDRF
                     MTHFAMQEADDSGSPVTWAEHVTDEEYGRAGR"
BASE COUNT          518 a          832 c          876 g          415 t
ORIGIN      
        1 acaatcgggc gccgccacgc cagcaggatc accgcgccga agaggatccg caggaatgcg
       61 gtgtggaccg gcccagcatc ctcaaccgcc ggcttggcga tgaccgagcc gagttggaat
      121 ccaaagatgg ccatcatgac cagcgccgaa gccggtaatc ggtccagcac ggatgggcga
      181 tgagtcaggg tcaattctgc cgcgggagca gccacgtctg gtaatccaat tcttacgtga
      241 acccggtcgt ccgggccccg aatgatcgca cacaggtcgg tcgcgagttg agggttgagg
      301 gaacggaaaa cggaaggcgg aaaaccggag acgagagacc agaaatcagg aaagatgaga
      361 gagctgcgca gccttcaacc cgtaccgctt aaccctcaac ccgtagaatg ggcgggctgc
      421 cgtcaacgga gacggtccaa caccggggga agacaattcg tgaagcttgc gattatcggc
      481 gtgccgttca attcggcggg gactcatacg gccgaggcgc tcgcgcccga tgccctgcgc
      541 gaagccggcc tcgtcaccgc gctcgcccgc aacaacgacg tcgccgacta cggcgatgtc
      601 agctttgagc cgcccagatc ggagaagcgg gaccgcgatt ccggcctggt cgcgccacac
      661 gcgctggtca gcatgaccgg cgcggtccgc gatgccgcca agcgcgcgta cggcgagggc
      721 cggatgccgg tcgtgatcgg cggagactgc gcgatcctgc tcggtggtct gctcgccgcg
      781 cgggaccagc ttggctatgg ccccgggctg ctcttcgtcg atggccacga ggacagctac
      841 gatccaaaga aatcgctcaa tggcgaggcg gccgacatgg agctcggcct cgcgaccggc
      901 ttcacgaagc tgaccggcct accggagctg gcggacgagc tcccgatcgt cgatccgcac
      961 gagatcgtcc tgatcggcgc gcgggacagc gctcaggtcg agagcgaggg ccacgccagc
     1021 attggcaccc gggtgatcgt gctcgacgac accgaactgc gcgctgccgg agtcgaatcg
     1081 acgattctgc gttggctgga acagttccag cacaagccgg gccgcttctg gttccacctc
     1141 gactgggatg tgctttccgc cgacgagatg ccggccgtct cctaccccca gccgggaggg
     1201 ctggcgtggg acgagctgga gacgatcgcc agggcggcgc ttcatgcaga tcacctgatc
     1261 gggatcgata tcacgctcta caacccgcag ctcgatccgg acgggaccgc ggcggggaag
     1321 atcgtcgcct tcctggccga tgtcaccggc gcgccggaac gagagccgcg cctcgggcgt
     1381 atctagaacg ggaatccgat ttctgttcaa ggatggttgt catgctcact cgttcgaccc
     1441 ggcgcacgtt cctgcaaacg accgctgccg gcctcgctgc catttcgatc cgaccccttc
     1501 gggcgcgcgc ggacgaggac gccgcgcaat tgatcgccgc ggctgcgaag gagatggcca
     1561 aggtcaagac gttccatttt gatatggaga ccgtcgacgg caagtccacg atcctcgaaa
     1621 acctcgagct caggaaggtc gtcggcgacg tggtgcggcc ggattcattc caggcgacgc
     1681 tgacggcgaa ggtcgcggtg atcaatgttt cggtagacgt catcagcgtc ggcggggccg
     1741 tctgggtgac cgatccgctc cagtcgggca aggtctggcg gcaggtggcc ggagacggcg
     1801 agcagaacat gggggctgcc ttcaccgagt tgatcaaccc ggaccgcctc ttcctctcgg
     1861 cggtctccct gatcgacgat ccgaagatcg acggcacgga gaaggtcaac ggcgaggatt
     1921 gcaccgtcgt gactggcgaa tttgacccga agcgactctc ggagctggcc agcccggtag
     1981 caggggagga ggtcgcaacg ccctcgatcc tgacgggcga gcccgtctac ctgtcggcgt
     2041 ggatcaacac cggtggacgg gtgctccgca tcgaggaaga agggccgctg actacgagcg
     2101 agtcgaacga cgtcgtccgc gcgatcacct tcagcgcctt tgacgagccg gtccagatca
     2161 ccgctcccca gtagcgctcg atcagggacg taaacgcgga ggcaacatgc agatcaccag
     2221 aaattccaga cagacgatgc cgggacccaa agagtggttc accggcgatg tatttatcga
     2281 caccgtggcg acgccggccg ggctgtctcg cgccggggca gcgatggtgc acttcacacc
     2341 gggggcgcgc actgcctggc atacccatcc acgtggtcag accatccacg tgactgaggg
     2401 gatcgggcgg tgccagcgcc gcggcgggcc aatcgaggtt attcaacctg gcgacagcgt
     2461 ctactttgaa ccgggcgagg agcattggca cggcgcggct cctgaccgtt tcatgaccca
     2521 cttcgcgatg caggaggccg atgactcggg cagcccggtg acgtgggctg aacatgtcac
     2581 cgacgaagaa tacggccgag ccggccgctg acctggccac cccaataatc gcatcgcaat
     2641 a
//
LOCUS       DASSGX010000500         3926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1178319, whole genome shotgun sequence.
ACCESSION   DASSGX010000500 DASSGX010000000
VERSION     DASSGX010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178319"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VFI12_08935"
     CDS             complement(<1..212)
                     /locus_tag="VFI12_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056575.1"
                     /translation="MTNANRLVAVLGRVREAGLLIALAAIVIGVTVSEPRFASVNNVR
                     QILLSISILAIVAIGQTLVVLTRNIDL"
     gene            complement(209..1756)
                     /locus_tag="VFI12_08940"
     CDS             complement(209..1756)
                     /locus_tag="VFI12_08940"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HET7056576.1"
                     /translation="MTDPAPSNPLPPAATPPVVELRDVTKQFGAVRALRGVQLVLYPG
                     EVHALVGENGAGKSTLVKMLGGIHQPDTGEILINGRPVALTSPLDARAHGIAVIQQHP
                     FLFPDLDVAENVFMGRQPKTRFGRIDWRRMNREVDTLLRPLGVSFAATTPVRGLSVAD
                     QQMVEIAKALSQDARVLVMDEPTASLSAREVERLFAIVRRLREQGAAILFVSHRLEEV
                     AELADRVTIFRDGGYVITAPTSELTTDQIIRHMVGRQLDSLFPKEAAEIGEVVLSVRG
                     LSRLGAFQDVSFDLRRGEILGLFGLVGAGRTEVARVIFGVDGAERGTVALNGRPVSIA
                     DPTDAMNHGIAYVPEDRHAQGLILTFPIDENITLPILRRLSNLTLVNSGKAEAIAADY
                     AARLQVKSAGMDQLVSALSGGNQQKVVLGKWLATDPVVLMLDEPTRGIDIGTKAEVHR
                     IISHLAGQGLAILMISSELPEILGMSDRILVMHEGRVSGEFSRVEADQERIMFAATGQ
                     VTTGAPA"
     gene            1900..2382
                     /locus_tag="VFI12_08945"
     CDS             1900..2382
                     /locus_tag="VFI12_08945"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET7056577.1"
                     /translation="MAMARDERQALIEQYATGYDAVLDALEGITAAEWESREAAGEWC
                     AREIAHHLGDSEMSAAVRVRALIADEAAVIQAFDQDRWNAELYPRDRPIEPSLAAFRA
                     AREATLPLLRLMTDEQWTREGQHSINGPMSAETWLEWYGPHAHGHADQIRRARAAAHG
                     "
     gene            complement(2814..>3926)
                     /locus_tag="VFI12_08950"
     CDS             complement(2814..>3926)
                     /locus_tag="VFI12_08950"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET7056578.1"
                     /translation="LDLIQVHRPSPVRAGRALALLHAALADTVVAVWDARAAYPRPSP
                     AQSDGQVTQLGSRGAAVSFPSEHAAVARCAASVLAYLFPNEPADGLTKLADEAAESRL
                     WAGAAYRSDIDAGTALGTAVADRAIARGTGDGSGRAWDSSGRPTGAGVWQPTPPGYVQ
                     APLDPMAGTWMPWLLPSGDAFRPAAPPANGSAAWQAELIAVQEAVARRTPEQAALVGQ
                     WAGGAGSVTPGGLWIQIASDVITRSGLDTPMAARALALTSVAIADAFICCWDAKFAYW
                     TERPITADPTLEVLIPTPPFPSFTSGHATASTAAATVLGHLFPDDEADLLAKAAEATQ
                     TRLWAGIHFPIDCDVGAAGGGMVGRLAVLRATADEG"
BASE COUNT          639 a         1367 c         1350 g          570 t
ORIGIN      
        1 aggtcgatgt tccgggtcag aacgacgagc gtctggccga ttgcgacgat ggccaggatc
       61 gagatcgaga gcaggatctg gcgcacgttg ttgacgctcg cgaagcgcgg ctcgctgacc
      121 gtgacgccga tcacgatcgc cgcgagcgcg atcagcagcc cggcctctcg cacccgtccg
      181 agcaccgcga ccagtcgatt ggcatttgtc atgccggcgc tccagtcgtg acctgcccgg
      241 ttgccgcgaa catgatccgc tcctgatccg cctcgacccg gctgaactcg ccgctgaccc
      301 ggccctcgtg catgaccagg atcctgtccg acatgcccag aatctccggc aactcactcg
      361 agatcatcag gatcgcgagc ccttgccctg ccagatgtga aatgatccgg tggacctccg
      421 ccttggtgcc aatgtcaatc ccgcgcgtcg gctcatccag catcaacacg accggatcgg
      481 tcgcgagcca cttgccaagc acgaccttct gctgattccc gcccgagagg gctgagacga
      541 gctggtccat cccggccgac ttgacctgca accgcgccgc gtagtcggcc gcaatcgctt
      601 ccgccttgcc ggaattgacc agcgtcaggt tggaaaggcg gcgcaggatc ggcagcgtga
      661 tgttctcgtc gatcgggaag gtcaggatca gtccttgcgc gtggcggtct tcggggacgt
      721 aggcgatgcc gtggttcatg gcgtcggtcg gatcagcgat cgacaccggc cggccgttga
      781 gggcaacggt cccgcgctcg gccccatcga cgccgaagat gacccgggcg acctcggtcc
      841 ggcctgcgcc gacgagcccg aacagcccca ggatctcgcc ccggcggagg tcgaacgaga
      901 catcctggaa cgcgccgagc cggctcaacc cgcgcaccga aaggacgacc tcgccgatct
      961 cggccgcctc cttgggaaag agcgaatcga gctggcggcc gaccatgtgc cggatgatct
     1021 gatccgtcgt cagctcgctg gtcggcgcgg tgatcacgta cccgccgtcc cggaagatgg
     1081 tgacccggtc ggcaagctcc gcgacctcct ccagccggtg cgagacgaaa aggatcgccg
     1141 cgccctgctc gcgcagccgg cggacgattg cgaagagccg ctcgacctcc cgggccgaga
     1201 gtgacgccgt cggctcgtcc atgaccagca cgcgggcatc ctgcgagagg gccttggcga
     1261 tctcgaccat ctgctggtcg gccacggaga ggccgcgcac cggcgtcgtg gccgcgaagc
     1321 taaccccgag cggccgcaac agcgtatcga cttcgcgatt catccgccgc cagtcgatcc
     1381 ggccgaatct ggtcttcggc tgccggccca tgaagacgtt ctcggcgacg tcgagatcgg
     1441 ggaagaggaa ggggtgctgc tggatgaccg ctatgccgtg ggcccgggca tcgagcgggc
     1501 tggtcagggc caccggtcgg ccgttgatca ggatctcgcc ggtgtccggc tgatgaatcc
     1561 cgccgagcat cttgacgagc gtgctcttgc cggccccgtt ctcgccgaca agggcgtgga
     1621 cttcgccggg atagagcaca agctggaccc cgcgcagcgc ccggaccgcg ccgaactgct
     1681 tggtcacgtc gcgcagctcg acgacgggcg gagtcgcggc tggtgggagc gggttcgatg
     1741 gcgctgggtc ggtcatgcct gctcgttccg gatcgattct gccgaattgt cacagcctac
     1801 cgcaaaagcg atgcgcgtgc gctctcgtcg cgcctggtct cgaccggcga tatgcttctc
     1861 atactgctgg cggcactcta caccgaagga gacgtgacta tggcaatggc acgcgacgag
     1921 cgacaggcgt tgatcgagca atacgcgacc gggtacgacg ccgtgctcga cgccctcgag
     1981 gggattacgg ccgcggagtg ggaatcgcgc gaggcggcgg gcgagtggtg tgcacgggag
     2041 atcgcgcatc acctcggcga cagcgagatg agcgcggcgg tccgcgtccg ggcgctgatc
     2101 gcggacgagg cggcggtcat ccaggcgttc gaccaggatc gctggaatgc ggagctctac
     2161 ccgcgggacc ggccgatcga accgtcgctg gccgccttca gggccgcccg cgaggcgacg
     2221 ctgcccctgc tgcggctgat gaccgacgag caatggaccc gagagggtca gcactcgatc
     2281 aacggcccga tgtcggccga aacctggctg gaatggtacg gcccccacgc ccacggccac
     2341 gccgaccaaa tccgccgcgc ccgcgccgca gcgcatggct gaggcgcgga ggcgcgacga
     2401 ttgtaggggc agatggcatc tgccctccgt tgcccaacgg cccggtcgca cgcggacggg
     2461 cgtgcccggg accgcccggg cgtaaacgcc cggactgaat ccaatacgcc cgctaaagca
     2521 ggctggaatc aggcgcagac cacaacatcc tcccttcgcc cacgtgtggg agaagggccg
     2581 gggatgaggg ccggtcatcc gcccggacgg tctcctgggc cgggccgggc gtggcgatgc
     2641 acgtggccat cgaccgcggc caggcgtgcc agacaccgac cctcacccca cccctctccc
     2701 gtgaacggga gagggagagt tggtgggcgg gtgcacgcga acgggccggg cggatgagcg
     2761 gcgggtttgc ccccgcaaac ccctacaccg tcgcgctcct actcctcgac tcctcaacct
     2821 tcgtcggcgg tcgcgcgcag gaccgcgagg cggccgacca tcccgccgcc ggccgcgccg
     2881 acgtcgcaat cgatcgggaa gtggatgccg gcccaaagcc gagtctgcgt tgcctccgca
     2941 gccttcgcca gcagatcagc ctcgtcgtcc gggaacaagt ggcccagcac cgtcgcggcc
     3001 gcggtcgagg ccgtcgcgtg gccggaggtg aacgagggga acggcggcgt cgggatcagc
     3061 acctccagcg ttggatcggc ggtgatcggc cgctcagtcc agtaggcgaa cttcgcatcc
     3121 caacagcaga tgaaggcgtc ggcgatcgcg acgctggtca gggcaagtgc ccgcgcggcc
     3181 atcggggtat cgagtccgga gcgagtaatc acgtcggatg cgatctggat ccagagtccg
     3241 ccgggcgtga cgctgcccgc cccgccggcc cactgcccga ccagcgccgc ctgttccggc
     3301 gtgcgccggg cgaccgcctc ctgcacggcg ataagctcgg cctgccaggc cgccgacccg
     3361 tttgccggcg gggcagccgg gcggaacgcg tcgccactcg gcaagagcca cggcatccat
     3421 gtgccggcca tgggatcgag cggcgcctga acatagccgg gtggggtcgg ttgccacacg
     3481 ccagcaccgg ttggccggcc gctgctgtcc caggcccgac cggagccatc gcccgtgccc
     3541 cgcgctatgg cccggtccgc gaccgccgtg cccagcgcgg tgccggcgtc gatgtcgctc
     3601 cggtacgccg cgcccgccca gagccgggat tcggcagctt cgtccgcgag cttcgtcaac
     3661 ccgtcggccg gctcattggg gaacagatag gccaacaccg acgcggcgca gcgggctacg
     3721 gccgcatgct cggacggaaa cgacacggcc gcacccctgc taccgagctg tgtgacctgg
     3781 ccgtcggact gggccgggga tgggcgcgga taggccgccc gggcgtccca gaccgccacg
     3841 accgtgtcgg ccagcgccgc atggagcagc gccagcgctc gtcccgcgcg gaccggactt
     3901 ggtcgatgga cctgaatcaa gtcgag
//
LOCUS       DASSGX010000501         6432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1285368, whole genome shotgun sequence.
ACCESSION   DASSGX010000501 DASSGX010000000
VERSION     DASSGX010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6432
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1285368"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /gene="galE"
                     /locus_tag="VFI12_08955"
     CDS             <1..804
                     /gene="galE"
                     /locus_tag="VFI12_08955"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HET7056579.1"
                     /translation="AAVRQAKPDAIMHFAAATVVPESVEDPLLYFEVNTVGSHYLISA
                     AIAAGVPRFVFSSTAAAYGDVDAEVLTEQMPTNPISPYGRSKVMVEQMLQWHQQRYHL
                     NVAIFRYFNVAGATGIRGEDHRPETHLIPVALLAALGKRDGLTVFGADYPTPDGTCIR
                     DYVHVADLADAHVLAADWLTDNGWGLFNLGTTSGTSVQQVIHAVEGVTDRTLPVTIGP
                     RREGDPARLIASAEKARTVLGWEPKRSTVEQMVGSAWDWMQHHPDGYAD"
     gene            801..2297
                     /locus_tag="VFI12_08960"
     CDS             801..2297
                     /locus_tag="VFI12_08960"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HET7056580.1"
                     /translation="MRGHAKTMIEEQRVAGDQVLEEIGEAGLQLPPEELRTLGYAVID
                     AIVEQAVTLREQPTARWADRAMMDAKLWESAPEHGTDAAAVLQRTLDDVMSAGHRNSH
                     PRYFGYVPNPSNLIGALADALASGFNIFSGMWMGAPGAAEVELLVTDWMREWCGLPKA
                     GGGVLLSGSSMANLVGIATARHARFGQDMEAARRGVIYYSDQAHSSVGKGARMIGIGP
                     DRIRRLPSDDQFRLSLDALAEAVRADRAGGLVPFCVIAAAGTTNTGAVDPLLELADFC
                     AAEGIWLHADGAFGAAAVLSESGKERLVGLDRVDSLAVDAHKWLFQPIECGCLLVRDV
                     RRLRETFHERPEYLRDADMTDAEVNFCDLGPQLTREFRALKLWMSIQVFGMGAFRKAV
                     SRGIALAELAERELRASGKWEMVTPAQLSVLTFRFAPAGCTDQELNALNASIAHAVLE
                     DGYAALSTTELRGQTVLRMCVTNPRATEAEIRETIRRLGEIADTQAAH"
     gene            complement(2733..3353)
                     /gene="plsY"
                     /locus_tag="VFI12_08965"
     CDS             complement(2733..3353)
                     /gene="plsY"
                     /locus_tag="VFI12_08965"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HET7056581.1"
                     /translation="METPLGILVLVLAYLAGAIPVGVILGKLLRGSDLRTIGSGSTGT
                     TNALRAYGPKVSAMVLIFDILKGAIPVLFAHALDRGPWIVGAVAVLTVVGHCWSPFIE
                     FDGGKGVATGAGALIALQPWVAFSIFLMVAIVLLTHYVSLGSIVTAVTIGIVLSIAAL
                     SDRVADPIAIAAVVIAAIIVWRHRGNIDRLIHGSERRISFRNRATA"
     gene            complement(3355..4692)
                     /gene="der"
                     /locus_tag="VFI12_08970"
     CDS             complement(3355..4692)
                     /gene="der"
                     /locus_tag="VFI12_08970"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HET7056582.1"
                     /translation="MARPIVAIVGRPNVGKSTLFNRLVGERRAIVEDVPGTTRDRLYG
                     TTTWGGVDFTIVDTAGLQDTSEIERSTTAEIGQMTRDQARSAIDEADVIVFMVDTKAG
                     MTAGDHEVADMLRRTDKPTILVANKVDNINRKDLAFEFYELGLGDPMAMSSYHGVGTS
                     DLLDRIVENLPHSEEEPEVAGPKIAIVGRPNVGKSRLVNALIGEERSIVSDIPGTTRD
                     SLDTHMTWNSQPLTLIDTAGIRRRGRIEQGIEHFSVLRSMRAIDRSDVVLMVVDSTEG
                     FTAQDLHIAGYVEEQKKGIVVVVNKWDLIEKDSSTMDLYRDEAKRQLDFLPYAPLVFI
                     SAKFGQRVQNVLEMALLVETERRKRVSTSALNKLLREAVAKHQPASKPGRWLKFYYAT
                     QADVSPPTFVFFCNDPQNVHFSYKRYLENQIRDQFGFVGNPVRMTFRGREEDE"
     gene            complement(4841..6187)
                     /gene="gcvPA"
                     /locus_tag="VFI12_08975"
     CDS             complement(4841..6187)
                     /gene="gcvPA"
                     /locus_tag="VFI12_08975"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HET7056583.1"
                     /translation="MTFNPHTAEDRAEMLAAVGVASVEELFDVIPDDIRRPNLHLPPA
                     LTEMEAAARLTELASKNLQIAPGNSFLGAGSYSHYVPALVNQLLLRGEFFTAYTPYQP
                     EVAQGTLQVIYEFQSMVAALLGSEVANASLYDGATAVAEAVLMATASVKKRNRVVMSG
                     TVHPHYREVLRTYVSGRDIAINELAVPEASSLLTPVDAIKSELNDEVACVVIQYPNFY
                     GGIEEVQAIADAVHEAGALLIVATLPVPLGLLTPPGEMGADFVTAEGQSLGIAQSFGG
                     PYVGLLGTRKSFVRQMPGRLAGIASDTEGKRGFVLTLQTREQHIRREKATSNICTNQG
                     LMATAATVYMSALGPQGFREVAERCYQNAHYLADQVTSLPGFERVGEGPFFNEFVVRT
                     LVAPSELNRRLAGKGIIGGLDLGRYEESLSGCWLLCATELNDKAAIDRLISALREI"
     gene            complement(6215..>6432)
                     /locus_tag="VFI12_08980"
     CDS             complement(6215..>6432)
                     /locus_tag="VFI12_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HET7056584.1"
                     /translation="VESVKAASDIYAPVTGEVVERNENAVAGPEVLNTSPYDDAWLIK
                     IKVSDQSALDALMSPDQYEKFLEEVGN"
BASE COUNT         1117 a         2027 c         2164 g         1124 t
ORIGIN      
        1 gccgctgtcc ggcaggcgaa acccgacgcg atcatgcact tcgccgccgc gacggtcgtg
       61 ccggagtcgg tggaggaccc gctcctctac ttcgaggtca atacggtcgg ctcccattac
      121 ctgatcagcg cggcgatcgc ggccggggtg ccgcgctttg tcttctcgtc caccgcggcg
      181 gcgtacggcg atgtcgatgc cgaggtgctg accgagcaga tgccgacgaa cccgatcagc
      241 ccctacggtc gctcgaaggt gatggtcgag cagatgctgc agtggcacca gcagcggtac
      301 cacctgaacg tcgcgatctt tcgctacttc aacgtggccg gcgcgaccgg gattcgcggt
      361 gaggaccacc gtccggagac gcatctgatc cccgtcgcgc tcctggcggc gctcggcaag
      421 cgcgacgggt tgacggtctt cggggccgat tacccgaccc cggacgggac ctgcattcgc
      481 gattacgtcc acgtcgccga tctggcagac gcgcacgtgc tcgcggccga ctggctgacg
      541 gataacggct ggggcctctt caacctcggc acgaccagcg gcacgtcggt ccaacaggtg
      601 attcatgcag tcgaaggcgt gacggatcgg acactgccgg tgacgatcgg gccgcgccgg
      661 gagggcgatc cagccaggtt gatcgcgtcg gcggagaagg cccggaccgt cctcggttgg
      721 gagccgaagc gctcgactgt cgagcagatg gtcggctcgg catgggactg gatgcagcat
      781 cacccggatg gatatgcgga ttgagaggcc acgccaagac gatgatcgaa gagcagcggg
      841 ttgcaggaga ccaagtgctg gaggaaatcg gcgaggcggg gctccagctt ccgcccgaag
      901 agttgcgaac gctcgggtac gccgtgatcg acgcgatcgt cgagcaggcc gtcaccctgc
      961 gcgagcagcc gacggcgcgc tgggccgacc gggcgatgat ggacgctaag ctctgggagt
     1021 ccgcgccgga gcacggaacc gacgctgcgg ctgtcctgca gcgcacgctg gacgacgtaa
     1081 tgtcggccgg ccaccgcaac agtcacccgc gctatttcgg ctacgtcccc aacccgagca
     1141 atctgatcgg tgcactggcg gacgccctgg catccggctt caacattttc tcgggaatgt
     1201 ggatgggcgc gcccggcgcg gctgaggtcg aactgctcgt gaccgactgg atgcgcgagt
     1261 ggtgcggcct gccgaaggcg ggcggcggtg tcctcttgag tggctcgtcg atggcgaacc
     1321 tggtcgggat cgccacggcc cgccatgccc gcttcggtca ggacatggaa gcggcccggc
     1381 gcggcgtcat ctattactcg gaccaggccc attcgtcggt cggcaagggc gcgcggatga
     1441 tcggcattgg cccggaccgg attcgcaggt tgccgtcgga cgaccaattc cggctctcgc
     1501 tcgacgcgtt ggctgaggca gttcgggcgg acagggccgg cggccttgtc ccgttctgcg
     1561 tgatcgcggc cgccgggacg acgaacaccg gcgcggtcga tccccttctg gagctggccg
     1621 atttctgcgc ggccgagggc atttggctgc acgccgatgg cgcgttcggt gcggcggcgg
     1681 tgctgtcgga atccggcaag gagcggttag ttgggctgga ccgggtcgat tcgctggcgg
     1741 tcgacgccca caagtggctc ttccagccga tcgagtgcgg gtgcttgctg gtccgcgatg
     1801 tccgccgcct gcgcgagacg tttcacgagc ggccggagta cctgagagac gcggacatga
     1861 ccgatgccga ggtcaacttc tgcgatctcg gcccgcaact cacccgcgag ttccgggcgc
     1921 tcaagctctg gatgtcgatc caggttttcg ggatgggcgc cttccggaag gccgtctcgc
     1981 gcggaatcgc ccttgcggag ctggccgaac gagagcttcg ggcatcaggc aaatgggaga
     2041 tggtcacgcc ggcgcagctc agcgtcctca cgttccgatt cgcaccggca ggatgtaccg
     2101 accaggagct gaacgcgctc aacgcgagta tcgcccacgc cgtgctcgaa gatggctacg
     2161 ccgccctcag cacgaccgaa ctgcgcggac aaaccgtgct ccgcatgtgc gtcacgaacc
     2221 cccgcgcaac cgaggccgaa atcagggaga cgattcggcg gttgggtgag atcgcggaca
     2281 cacaagccgc tcattgacgc gggagccata cacaagtagg ggcagatggc atctgccctc
     2341 cggtcccgat cggcgcggtt gcataggaac gttcgtgccc gggaccgccc gggcgtaaac
     2401 acccgggctg aatccaataa gcccgctgaa gcaggctgga tagaggcgcg gcgttccatg
     2461 aaatcctccc ttctcccgca tgcgggagaa gggccgggga tgagggccgc tcatccgctc
     2521 gcatcatgtc ttgagccggt ccggtcgtag ggatgcatgg tttcggcaaa cgaccgtgcc
     2581 cgacaccggc cctcaccccg ccccggtccc gtaaacggga gagggagata tgtggaatgg
     2641 gcgagcagct gcgaacgggt cgttgcgcag tggcgggcag atgccacctg cccctacatc
     2701 gtgggcgcac tccgacattc ggatcggagc gttcaggccg tggcgcggtt gcggaaggaa
     2761 atccggcgtt ctgagccgtg gatcaggcgg tcgatattgc cgcggtggcg ccagacgatg
     2821 atcgcggcga tgaccacggc cgcgatggcg atggggtcgg cgacgcgatc cgacaacgcc
     2881 gcgatcgaga ggacgatgcc gatagtgacg gcagtgacga tcgagccgag cgagacgtag
     2941 tgcgtcagca gcacgatcgc gaccatcagg aagatcgaga atgcgaccca cggctggagg
     3001 gcgatcagcg cgccggcgcc cgtcgcgacg cccttgccgc cgtcgaactc gatgaagggc
     3061 gaccagcaat ggcccacgac ggtcaggact gcgaccgcgc cgacgatcca cggtccgcga
     3121 tcgagggcgt gcgcaaacag gaccgggatg gcgcccttga ggatgtcgaa gatgagcacc
     3181 atcgcgctga ccttcggccc ataggcgcgc aaggcgttcg tagtgccggt tgagccgctg
     3241 ccgatggtgc ggaggtcgct gccgcggagc aacttgccga ggatgacgcc gaccgggatc
     3301 gcgccggcca gatatgcgag taccagaacc aaaatgccga gcggcgtttc caaactattc
     3361 gtcctcttcc ctgccccgga acgtcatgcg taccgggttc ccgacgaacc cgaattgatc
     3421 ccggatctga ttctccaggt atcgcttgta ggaaaaatgt acattctgcg ggtcgttgca
     3481 gaagaagacg aacgttggcg gcgatacatc cgcctgcgta gcgtagtaga acttgagcca
     3541 cctgccgggc ttgctcgccg gctgatgctt cgcgaccgct tcccgaagca gcttgttcag
     3601 cgctgacgtc gagacgcgct tgcgccgctc cgtctcgacc agcaacgcca tctcgagcac
     3661 gttctgaact cgctggccga atttggccga aatgaaaacg agtggcgcat acggcaggaa
     3721 gtcgagctgg cgcttggcct cgtcgcgata gaggtccatg gtcgaggagt ccttctcgat
     3781 caggtcccat ttgttgacga cgacgacgat gcccttcttc tgctcctcga cgtaccccgc
     3841 gatgtgcaga tcctgagccg tgaacccttc ggtcgagtcg accaccatca gcacgacgtc
     3901 agaccggtcg atcgcccgca tcgaacggag cacgctgaag tgctcgatgc cctgctcgat
     3961 ccggccccgg cgacgaatgc cggccgtgtc gatcagggtc aatggttggc tgttccaggt
     4021 catgtgcgtg tcgaggctgt cgcgcgtggt gccgggaatg tcgctgacga tcgagcgttc
     4081 ctcaccgatc agcgcattca cgagcctgct cttgccgacg ttcggtcgcc cgacaatcgc
     4141 gatctttggc ccggccacct ccggttcttc ctcggagtgc ggcaggttct cgacgatccg
     4201 gtcgagcagg tcgctggtgc caacaccgtg atacgaggac atcgccatcg ggtcaccgag
     4261 gccaagctcg tagaactcga aggcgagatc cttgcggttg atgttgtcga ccttgttggc
     4321 gacgaggatc gtcggcttgt cggtgcgccg gagcatgtcg gcgacctcgt gatcccccgc
     4381 ggtcattccg gccttggtgt cgaccatgaa gacgatcacg tccgcctcgt cgatggcgct
     4441 gcgagcctga tcccgggtca tctgcccgat ctcggcggtt gtcgaccgct cgatctccga
     4501 cgtgtcctgc aggccggccg tatcgacgat ggtgaagtcg accccgcccc aggtcgtcgt
     4561 gccgtagagc ctatcgcgcg tggtgcccgg gacatcctcg acgatggccc gccgctcgcc
     4621 gacgagccgg ttgaagaggg tggatttgcc gacgttcggt cggcctacga tcgcgactat
     4681 agggcgtgcc acagtatcac cagcgtgtcg tacaagcgtt caaaggtatg aggcagggag
     4741 aatcagtgcc tgcctggagt cgttcgggct acgaatgaca cgggaggtga gtatagcaaa
     4801 gcgatgcgca ataccggccg atctcagccc gacctttctc ctaaatctcg cggagcgcgc
     4861 tgatgaggcg gtcgatcgca gccttgtcgt tgagctcggt cgcgcagagg agccagcacc
     4921 cggacagaga ttcctcgtag cggccaaggt cgaggccgcc gatgatgccc ttaccggcca
     4981 gcctccggtt gagctcggac ggggcgacca gcgtgcggac cacaaactcg ttgaagaacg
     5041 gaccctcgcc gacgcgctcg aaaccgggca gcgatgtgac ctgatcggcc aggtaatgtg
     5101 cgttctggta gcagcgctcc gcgacctcgc ggaagccctg agggccgagc gccgacatgt
     5161 agaccgtcgc ggccgtcgcc atcagtccct ggttagtgca gatgttgctg gtcgctttct
     5221 cgcggcggat gtgctgctcg cgcgtctgga gcgtcaggac gaagcctcgc ttgccctcgg
     5281 tatcgctcgc gatcccggcc aggcggcccg gcatttggcg cacgaatgac ttgcgggttc
     5341 cgagcaggcc gacataggga ccgccgaagc tctgcgcgat accgagcgac tgcccctcgg
     5401 ccgtcacgaa gtcggcgccc atctctcccg gaggtgtcag caggccgagc ggcaccggca
     5461 gcgtcgcgac gatcaacagc gccccggcct catggaccgc gtccgcgatc gcctggacct
     5521 cctcgatccc gccgtaaaag ttcgggtact ggatgacgac gcaggcgacc tcgtcgttca
     5581 gctcggactt gatcgcgtcg accggcgtga gcaagctcga cgcctcggga acggccagct
     5641 cgttgattgc gatgtcacgg ccgctgacat aggtccggag cacctcgcgg tagtgcgggt
     5701 ggacggttcc ggacatcacc acgcggttgc gcttcttgac ggaggcggtt gccatcagga
     5761 ccgcctcggc cacggcggtg gcgccgtcat agagcgaggc gtttgcgact tcggagccga
     5821 gcagggccgc gaccatgctc tggaactcgt agatcacctg cagcgtgcct tgcgccactt
     5881 ccggctggta gggcgtgtag gcggtgaaga actcgccgcg gagcaggagc tggttaacca
     5941 gcgccggcac atagtgactg tagctgccgg ccccgaggaa gctattgccc ggcgcgatct
     6001 gcaggttctt gcttgccagc tcggtcaggc gcgccgcagc ctccatctca gtcagcgccg
     6061 gcggcaggtg aagattcggc cggcggatat catcggggat gacgtcgaac agctcttcga
     6121 ccgacgcgac gccaaccgcc gccagcatct cggcgcggtc ctctgcggta tggggattga
     6181 atgtcatgaa cggagcgacc gggcctttcc ggaactagtt accaacctct tcgaggaact
     6241 tttcgtattg gtcgggcgac atgagagcat cgagcgcgct ctggtcgctg accttgatct
     6301 tgatcaacca tgcatcgtca tagggagatg tgttcagaac ttccggcccg gcgaccgcgt
     6361 tctcgttgcg ctcgacgacc tcgccggtga ccggagcgta gatgtcggac gcggccttga
     6421 ccgattcgac ga
//
LOCUS       DASSGX010000502         2724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_196675, whole genome shotgun sequence.
ACCESSION   DASSGX010000502 DASSGX010000000
VERSION     DASSGX010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196675"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1302)
                     /locus_tag="VFI12_08985"
     CDS             complement(<1..1302)
                     /locus_tag="VFI12_08985"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 domain-containing protein"
                     /protein_id="HET7056585.1"
                     /translation="MSSHREAPEISKDPVADNTDVYAFVSPDQPDTVTIIANYIPLEE
                     PAGGPNFFQFGDDVLYTLYVDNDGDAEPDITYEFRFQTQIQNPDTFLYNTGPITALDS
                     PNWNVRQTYSVTRIEGLKSYRRRSVGKTAVLASNLMTPPCRIGPRSTPDYEALAEAAV
                     YELPTGQTVFAGQRAEGFFVDLGSIFDLGDLRPFQNLHLIPSAAAAGVNGTKGFNVHT
                     IALRVPKTELTQDGSNPTDPTDQRSVVGIWAAANRSKVTVRLNAKQPNVSTGPWVQVS
                     RLGNPLFNEVIVPLGDKDAWNLLDPADDQQFLEYVEHPELARLLPVLYPGVFPHLASL
                     SADRADLVAILLTGIPAGIIPGFQNYTGATNADVLRLNMAIPPTADPNPFGILGGDLA
                     GFPNGRRVFDDTVNIELRAVAGATIPFVDPSFTPDAAVSAIT"
     gene            complement(1351..1623)
                     /locus_tag="VFI12_08990"
     CDS             complement(1351..1623)
                     /locus_tag="VFI12_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056586.1"
                     /translation="MTSTISRRMFLTRASTGAVALGALGSVSGLAAAAPAHTGVTEEP
                     PEIALTEPFVVYIHDPSAGEVAIISGTQEVTRHDPVLVAHLLNLMG"
     gene            complement(2027..2638)
                     /locus_tag="VFI12_08995"
     CDS             complement(2027..2638)
                     /locus_tag="VFI12_08995"
                     /inference="COORDINATES: protein motif:HMM:NF004813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET7056587.1"
                     /translation="MQILEATATKANLMDRFGLTRRLVDLLHEGEAIVGGIGNANFDL
                     FGAGDRPENFYMLGSMGMAVPIGLGVAMAQPERQVIVLEGDGSLLMSLGCLSTIGMVA
                     PPNLTVVVWDNGAYQMTGGQPTPAAATTDLVQVAQAAGIANSNWAQDEQHFERLVQQA
                     LWSEGPSFIGAGVLPTASQSHPEFDPVILKDRFMRGIGAKPAR"
BASE COUNT          506 a          797 c          893 g          528 t
ORIGIN      
        1 ggtgatcgcc gagaccgccg cgtcgggggt aaagctggga tcgacgaatg ggatcgtcgc
       61 cccggcgaca gcgcgtagct cgatgttgac cgtgtcgtcg aagacacgcc gaccgttcgg
      121 gaacccggcg agatcgccgc ccaggatacc gaagggattc ggatcggctg tcggaggtat
      181 cgccatattc aggcgtagca cgtcagcgtt cgtcgcgccg gtgtagttct ggaaacctgg
      241 gatgatcccg gccgggatgc cggtcaagag aatcgccacg aggtcagcgc gatccgcact
      301 gagcgaagcc agatgtggga agactccggg atagaggacc ggcagcagcc tggcgagttc
      361 tggatgctca acatactcca ggaattgctg gtcgtctgcc ggatcgagca agttccaggc
      421 gtccttgtcg cccagcggga cgatcacttc gttgaagagc gggttgccga gccgggaaac
      481 ctgcacccac gggccggtac taacattcgg ctgtttcgcg ttcaatcgca cggtcacttt
      541 gctccggttg gcggccgccc agatgccaac caccgatcgc tggtcggttg gatcggtcgg
      601 attggagccg tcctgggtca gttccgtctt gggcactcgc aaggcgatgg tatggacgtt
      661 gaaccctttg gtgccgttga ctccagccgc cgcggcagac gggatcagat ggaggttctg
      721 aaacggtcgc aggtcaccca ggtcgaagat cgaaccgagg tcgacaaaga agccttcggc
      781 tcgttgtccg gcgaacacgg tttgaccggt gggtagttca tacaccgccg cctcggccag
      841 cgcctcataa tccggtgtcg agcgcggccc gatacgacag ggcggagtca taagattgct
      901 tgccagcacg gccgttttgc ccactgaacg tctccggtaa gatttcagac cctcaatccg
      961 tgtgaccgag tacgtttggc gcacgttcca gttgggactg tcgagtgccg tgatcggtcc
     1021 cgtattgtag aggaacgtat cggggttctg gatctgcgtt tgaaagcgga actcgtaggt
     1081 aatatcgggc tccgcgtcgc cgtcattgtc gacgtagagt gtgtagagca catcgtcgcc
     1141 gaactggaag aaattcggac cgccggctgg ctcctcgagg gggatgtagt tggcaatgat
     1201 cgtcacggtg tcgggctgat cgggactcac gaacgcgtag acatcggtgt tgtccgctac
     1261 tggatctttt gaaatttcgg gtgcctcgcg atgggacgac acggtctgcc tcctgtcata
     1321 acgaacgggc tcgttcaacc ggaaccgctt ttagcccatg aggttcagca gatgggcgac
     1381 gagcacggga tcgtgtcgcg taacttcctg cgtgccgctg ataatcgcga cttcccccgc
     1441 ggagggatcg tggatataaa ccacgaacgg ctccgtgagc gcgatctcgg gcggctcctc
     1501 agtcacaccg gtgtgggccg gcgcggctgc ggcgaggcca gacacgctgc cgagcgcccc
     1561 gagggccacg gcgccagtgg acgctcgcgt gagaaacatg cggcgcgaaa tggtcgatgt
     1621 catgagcacc tcaccttagc gatccggcga cgctcatgca caaaacgtcg ccaacggaca
     1681 gccaaaactc gtccttcgat cgccttcacg cagtacacgg cattgcggga tcgtgctgaa
     1741 acgtgcctgt cattctcgtg agtggagcca gcgtaccgca gattttggcg acggaatggc
     1801 atctagtatg cgcgtttagc aaggcgagtc aagaaataat cgttcagcgg gagtgacaac
     1861 gtgttcatgt ggcaggaaga gaggcaggcg cctccaatcc ctcttcccgc accgcgcggg
     1921 agagagggga cttggggtga gcgcgtgtgg gttccggcga gacgcgagtg atgggtgacg
     1981 agggcgccat gcatggcgcc ctcgcggctt cacccgcgcg gatcttctat ctcgccggct
     2041 tggcgccgat gccgcgcatg aagcggtctt tgaggatgac ggggtcgaat tcggggtgcg
     2101 attgactggc ggtcggcagg acgcccgcgc cgatgaagga tgggccctct gaccagagcg
     2161 cctgctgcac gaggcgctcg aagtgctgtt catcctgcgc ccagttgctg ttggcgatgc
     2221 cggcagcctg cgctacctgc accagatcgg ttgtggcagc agcgggcgtc ggctggccgc
     2281 cggtcatttg ataggcgccg ttgtcccaaa cgaccacggt gagattcggc ggtgcgacca
     2341 tgccgatcgt gctgaggcag ccgaggctca tcaagagcga gccatcgcct tcgagcacga
     2401 tcacctggcg ctccggctgc gccatggcga cgccgagtcc gatcggcacc gccatgccca
     2461 tgctgccgag catgtaaaaa ttctccggcc gatcgccggc accgaagaga tcgaagttcg
     2521 cgttcccaat gccaccgacg atcgcctcac cctcgtggag caggtcgacc agtcgtcggg
     2581 tcagcccgaa gcgatccatc aaattcgctt tggtcgctgt ggcctctaga atctgcatct
     2641 gcaggtttcc tttcgctgac gcgcgcgcat ctacgtgcta agccgctcgc ttggacggtg
     2701 ccgaaccggg cgacgtcttg ccgc
//
LOCUS       DASSGX010000503         3589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_305458, whole genome shotgun sequence.
ACCESSION   DASSGX010000503 DASSGX010000000
VERSION     DASSGX010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305458"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="VFI12_09000"
     CDS             <1..565
                     /locus_tag="VFI12_09000"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET7056588.1"
                     /translation="SRSPGAMTTPRPESAGAPRRSPRAEAIVAAARQILEAEGRDALT
                     MRRLGAAVGIRAPSLYKHFPDKSSVEIALIETGFVELAAALESAAARDGSLPGVGAAY
                     RGYARDHPHLYRLITSGPLPRERLRPGVEERAAAPILAVTGDPDLARACWAFAHGMVI
                     LELDRRFPPGADLDSAWSAGIAALDRR"
     gene            664..1482
                     /locus_tag="VFI12_09005"
     CDS             664..1482
                     /locus_tag="VFI12_09005"
                     /inference="COORDINATES: protein motif:HMM:NF025049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET7056589.1"
                     /translation="MPNRKQDRPHWIDRLAELAARTDESRISRRLLGAAGVSAVAGAR
                     VQPNPARAMLAVETDSILGDAATAEGLLVTLLGVARTKQSQLTLDEATVRLLRAAQCE
                     EEAHFNNLVAAGAVPSTSQYTIADQLFENPTTFLTTWSDLERIMVGMYMIAARRLAEN
                     GEADLVEMTYQIGAVEAQHQALLRQILGDRLPADRAFAAWQFRQTSQALSHVKRMGFI
                     GGEGQTYDYPGPGDRYCRGVTGLVAETTSGQTPPDVTPAPSQEATPQASPISNS"
     gene            1515..2255
                     /locus_tag="VFI12_09010"
     CDS             1515..2255
                     /locus_tag="VFI12_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056590.1"
                     /translation="MSTTPPTTGPDDEQQPAPIIEDEEYPVDRLRSRLNESLGGRPLT
                     VYLVLFAGAATLLLLLAVVWISAQGGGEKDELICTEVSPADARAAVLSGQVQRINILV
                     DKDDPMQSLTGVQLRFSDGSCRQTPQGAALRDELFSIIGAVNLFNEYSDSSIRIHYQS
                     QDIQLELLITPTPTTISTETPSPTVTTEPTATSEPTATPSPSPTHTAAPTHTAAPTQP
                     ATASPTRAAPTVTPTASGQRVPVGNPGA"
     gene            2338..2583
                     /locus_tag="VFI12_09015"
     CDS             2338..2583
                     /locus_tag="VFI12_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056591.1"
                     /translation="MWKRTHLRDMSFWGQVVFGAGIVALGLLTLITLMFGSVEGTLVS
                     LVLLVLYGVSAPFVIAGHWRAPKPQPTRRPVVRRRRS"
     gene            complement(2622..3092)
                     /locus_tag="VFI12_09020"
     CDS             complement(2622..3092)
                     /locus_tag="VFI12_09020"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET7056592.1"
                     /translation="MAMSNPVPDPANATEHGLGTLLRISYQVMMADGVEPALAAAGYG
                     DIRSAHLPVIQALATNPAGLRATELATHARITKQSMGYLVDHLTAGGYVERLPDPSDQ
                     RAKVVCLTPRGLELSRTIRTAVRNVETSWAARVGSKHMQQLRTVLQELVASFQR"
     gene            3238..3432
                     /locus_tag="VFI12_09025"
     CDS             3238..3432
                     /locus_tag="VFI12_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056593.1"
                     /translation="MTFTLTLELPTATGATFAIEPDLRFAAIERDHQIHVIPLAGQMA
                     PPLVGDSVEPEPTWEDAAWQ"
BASE COUNT          612 a         1284 c         1110 g          583 t
ORIGIN      
        1 gtctcggagc cccggcgcaa tgacgacccc gcgcccggaa tcggccggcg cgccccgccg
       61 aagcccgcgc gccgaagcca tcgtggcggc ggctcgccaa atcctcgaag cggaggggcg
      121 cgacgccctg accatgcgcc ggctgggggc tgcggtcggc atccgcgctc cctcgctcta
      181 caagcacttt ccggacaagt cctccgtgga gatcgcgctg atcgaaaccg gcttcgtgga
      241 actggccgcg gccctcgaat ccgccgccgc ccgggatggc tcgctccccg gggtgggtgc
      301 ggcctatcgc gggtatgccc gtgaccaccc acacctctat cgcctgatca cctccggccc
      361 gctcccgcgc gagcgtctcc ggccgggcgt tgaggagcgg gcggccgcgc cgatcctggc
      421 cgtaaccggc gatccggatc tggcgcgggc ctgctgggcc tttgcccacg gcatggtgat
      481 tctcgagctc gaccggcgct ttccaccggg cgccgatctg gattcggcct ggagtgccgg
      541 aatagccgcg ctcgaccgcc gctagaccgg cggatcgctc cgttgccaac gccggtggcg
      601 aaccccggca caccgggtat gatgtgtacg gacacttcct gcgtctcgga gataagcagg
      661 gcaatgccga atcgcaaaca ggatcgaccg cactggatag accggctcgc ggagctggcc
      721 gcgcgcactg acgagtcgcg catcagccgc cggctgctcg gcgcggcagg cgtgtccgcc
      781 gttgctggtg ccagagtcca gccgaatccg gcccgcgcca tgcttgcggt cgagaccgac
      841 tcgatcctgg gcgacgccgc cacggctgaa gggttgctcg ttacgctcct cggcgtcgcc
      901 cgcacgaagc agtcccagct cacgctcgac gaagcgacgg ttcgcctgct ccgcgccgcc
      961 cagtgcgagg aggaggccca cttcaacaac ctcgtcgccg ccggcgcggt cccctcgacc
     1021 agccagtaca cgatcgccga tcaactcttc gagaatccca ccaccttcct aacgacctgg
     1081 tccgacctcg agcggatcat ggtcggcatg tacatgatcg ccgcccgccg ccttgccgag
     1141 aacggcgaag ccgacctcgt cgagatgacc taccagatcg gcgcggtgga agcgcagcac
     1201 caggccctgc tgcgccaaat cctcggtgac cggctcccgg ccgaccgggc gttcgctgcc
     1261 tggcagttcc ggcaaacgtc gcaggccctg agccatgtca agcggatggg cttcatcggc
     1321 ggcgagggtc agacctatga ttaccccggg ccgggggatc ggtactgtcg cggcgtgacc
     1381 ggcctggttg ccgagacgac atccggtcag accccgcctg acgtgacccc cgcacccagt
     1441 caggaggcga cgccgcaggc atcgccgatc agcaactcgt agcgcatgcg ggatttcgcc
     1501 ggggactgtc aggtttgagc acgacgccac caaccaccgg gccagatgac gagcagcaac
     1561 cggccccgat catcgaggat gaggaatatc cggtcgatcg cctgcgatcg cggctgaacg
     1621 agtcgctcgg cgggcggccg ctgacggtct atctcgttct cttcgccggc gcggccaccc
     1681 tcttgctctt gctggcggtc gtctggatat ctgcccaggg aggcggcgag aaggatgagt
     1741 tgatctgcac tgaggtttcc ccggccgatg cccgcgccgc cgtgctcagc ggccaggtcc
     1801 agcggatcaa tatcctcgtc gacaaggacg atccgatgca gagcctgacc ggcgtccagc
     1861 tccgtttcag cgacggttcc tgccggcaaa cgccgcaagg cgccgcgctc cgggacgagc
     1921 tgttcagcat catcggtgcc gtcaacctct tcaacgagta cagcgacagc agcatccgaa
     1981 tccattacca gagccaggac atccagctcg agctgctgat cacgcccacg ccgacgacca
     2041 tctcgaccga gacgccgtct ccaaccgtca ccacggagcc caccgccacg tcggagccaa
     2101 cagcaacgcc gtcgccgtcg ccgacccaca ccgcagcgcc gacgcacacg gccgcgccga
     2161 ctcagccggc caccgcatcg ccgacacgcg cggctccgac tgttacgccg acggccagcg
     2221 gacaacgcgt cccggtcggg aatccgggtg cataaagatc atccgaaact cccccgcgct
     2281 ctgctcgcca cggtctcccg tcgccgctag tgtgttccag gggggtggaa ccagacaatg
     2341 tggaagcgaa cgcacctgcg ggacatgtcg ttctggggac aggtggtctt tggcgccggc
     2401 atcgtcgcgc tgggacttct gacgctgatc acgctcatgt tcggctcggt agagggaacc
     2461 ctggtctcgc tggtgctact ggtgctctac ggcgtttctg ccccattcgt gatcgccggc
     2521 cactggcgcg ccccaaaacc gcagccgact cgccgccccg tcgtccgtcg ccggcgctcg
     2581 taacgtctcg tcaacgcttc atcacgcgct gagtagccag gtcaccgctg gaagcttgcg
     2641 accagctcct gcaatacggt gcgaagctgc tgcatgtgct tgctcccgac ccgcgctgcc
     2701 cacgacgttt cgacattgcg taccgccgtc cgaatggtcc ggctcagctc caggccgcgt
     2761 ggggtcaggc agacaacctt cgcccgctgg tcgctgggat ccggcagccg ctcaacgtag
     2821 ccacccgcgg tcaggtggtc caccaggtag cccattgatt gcttggttat ccgggcgtgg
     2881 gtcgccagct cagtggcccg caggcccgcg ggattcgtcg ccagcgcctg gatgacgggc
     2941 aggtgagcgc tccggatatc gccatacccg gccgcggcca gcgccggctc cacgccgtcc
     3001 gccatcatta cctgatagga aatccgcagc agcgtcccca ggccatgttc agtcgcattt
     3061 gccggatctg gaaccgggtt ggacattgcc attaccccac caccccttga cagtatggta
     3121 agacagtctt atcatctagt cagatagact taccacatgc taccacacgg cgtggcagcg
     3181 cgaccgccaa gcggcaacca cgaaccgggt gccggacgtc agagggagga caatgagatg
     3241 acgtttacac tgaccctgga gctcccaacc gcgacagggg cgacgttcgc gatcgagccg
     3301 gatctgcgat ttgcggccat cgagcgcgat caccagattc acgtcatccc actcgccggg
     3361 cagatggctc cgccactggt cggtgactcc gttgagcccg agcccacgtg ggaggacgcc
     3421 gcctggcaat aaacgactcc gcacgaatca ccgttcgcag cgtttcgcac cggtgtttga
     3481 ttccagcagt accagggagg cagtcatgat ccagccctca gcggacatcg cgatcgatcc
     3541 agccgacgaa gtgatccagc ttggtcccct cggaatccgc ttcctcgtg
//
LOCUS       DASSGX010000504         2288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1178649, whole genome shotgun sequence.
ACCESSION   DASSGX010000504 DASSGX010000000
VERSION     DASSGX010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1178649"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(249..1238)
                     /locus_tag="VFI12_09030"
     CDS             complement(249..1238)
                     /locus_tag="VFI12_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056594.1"
                     /translation="MNDGAFSHPINRRQFALGSAAGLAALTFLPARAMLVSAQDAGDL
                     SAMGYPELAVTVTDSSFEGVPDSTAAGRYLLTLTSSLPAGDTSNSGAIGFMSGTSLGM
                     SASDLLQFLASTGGPPPSGTPEAGASPAAGGDGEEGALPLEVYQLHFAGGVFAPPGQT
                     AQAVIDLTPGEYVVWGDDPSLPQKPVTMTVTGDFPTDVKEPTSDITATLVDFAITLEG
                     SLTAGKHIMKVQHHGAQPHFLDIEKGPDTMTKEMVMAAFMGEMSGTPTAGGMSESDMQ
                     PVFYSPTQSIGTTTWHMIDLTAGTFLAACFFPTAGTGVPHAMNGMVDVFKVTG"
     gene            complement(1700..>2288)
                     /locus_tag="VFI12_09035"
     CDS             complement(1700..>2288)
                     /locus_tag="VFI12_09035"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET7056595.1"
                     /translation="MIEPLATGTHAVRVAGGTDGKRVAVLGSGSIGLLTMQAARAGGA
                     ATIVVTDLSPAKRQRALDLGADAAFDPRDPNVVEAMRERLGGPADVVFDCVAIQATMD
                     QAIALAHKGGTVIVEGVPEADVTVPLAIIQDREIRIQGTAMYTREDMRSAMNLIADDK
                     VDAASLVTKTLPLDQAADAFAAADAGNEIKVHLRV"
BASE COUNT          368 a          719 c          799 g          402 t
ORIGIN      
        1 ctcggtcacg gccgagtgcc tgccaccggg catttcggcg aggggagggc agatgccatc
       61 tgcccctact tccggtccct cctgatatcg gtgtgggcgc aagggggtgc cgatagcgcg
      121 ccggtctccg cctgaccgac ccggcgtaat ccggcaaagt gacggccccc ggggttcacg
      181 ggggccgtcg tgttggtcca cgctgctcga gtagtgcgtg gactgtgagg taggaggggg
      241 agtctgggtt aaccggtgac cttgaagacg tcgaccatcc cgttcatggc gtggggcacg
      301 ccggtgccgg cggtcgggaa gaagcaggcg gcgaggaatg tccctgcggt caggtcgatc
      361 atatgccagg tcgtcgtgcc gatcgactgg gttgggctgt agaagaccgg ctgcatgtcc
      421 gattcagaca tgccaccggc cgtcggcgtg cctgacatct cgcccatgaa ggcggccatg
      481 accatctcct tggtcatggt gtccggcccc ttctcgatgt cgaggaagtg cggctgggca
      541 ccgtggtgct gcactttcat gatgtgcttc ccggccgtga ggctcccctc cagcgtgatc
      601 gcgaagtcga ccagggtggc ggtgatatcg gaggtcggct ccttgacatc cgtcggaaag
      661 tcaccggtca ccgtcatcgt cacgggcttt tgtgggagtg aaggatcgtc tccccagacg
      721 acgtactcgc cgggggtcag gtcgatcacg gcctgagcgg tctggcccgg aggcgcgaag
      781 accccgccgg cgaaatggag ctggtaaacc tccagcggca gcgcgccctc ctccccgtca
      841 ccgcccgctg cggggctggc gccggcctca ggcgttccgc tcggtggcgg gccgccggtg
      901 ctggccagga actgcagaag gtcggatgcg ctcatgccga gcgatgtccc tgacatgaag
      961 ccgatcgcgc cggaattgct ggtgtccccg gcgggaaggg agctggtcaa cgtgagtagg
     1021 tagcggccgg ccgcggtcga atccgggacc ccttcgaaac tgctgtcagt cacggtgacc
     1081 gcgagctcgg ggtagcccat tgccgagagg tcgccggcat cctgggcgga tacgagcatc
     1141 gcgcgggcgg gcaggaaggt gagtgcggca aggccggcgg cgctgcctag cgcaaactgg
     1201 cggcgattga tcggatggga aaacgctcca tcattcacgg actaacgctc cttggtgatt
     1261 acaacgaggg accggtcagg cccttgcctg gcgatcccga agacaaatgg acgccgtggc
     1321 cgagttcccc ttctcactcc ccttcgcgca tgattcatgt tggcccccgg cgctgcatat
     1381 cgctatacga tgaattgttc ggagaggttt tctgcgacag gcatattcaa tttgtccggt
     1441 ccgccgacgg agctcgccgc tatggtgacg gccgccggcg acgccggagc ccgtgtgtca
     1501 gatctccgct gttcgaggcg aaacggaagc gacaagccgg tatacgacac gtatcaccgg
     1561 tcggttttgc gaggcggggc gcgaaagcgg gagatgggtt gagggtcgct gggcgttggg
     1621 agatggacac ggaaagccga aatcgaaaga cgagagacca aagacccgag cctgatcccc
     1681 tcaaccctcc acccgggtct cagacgcgga ggtggacctt gatttcgttg ccggcgtcgg
     1741 ctgcggcaaa ggcatcggcc gcctggtcga gcgggagcgt tttcgtcacg agcgaggccg
     1801 cgtcgacctt gtcgtccgcg atcaagttca tcgccgagcg catgtcctcg cgggtgtaca
     1861 tggccgtgcc ctggatccgg atctcgcggt cctggatgat cgcgagcggt acggtcacgt
     1921 ctgcctcggg cacgccctcg acgatgacgg tgccgccctt gtgcgcgagg gcgatcgcct
     1981 ggtccatcgt cgcctggatc gcgacgcagt cgaagacgac atcggccggg ccaccgagcc
     2041 gctcccgcat cgcctcgacc acgttcggat cgcgcgggtc gaaggcggcg tcggcgccga
     2101 ggtcgagcgc gcgctgccgc ttggccgggc tcagatccgt gaccacgatc gtcgccgcgc
     2161 cgccggccct cgccgcctgc atcgtcagca gcccgatcga gccgctaccg agcaccgcga
     2221 cccgcttacc gtcggtaccg ccggcaacgc gcacggcatg ggtaccggtt gcgaggggct
     2281 cgatcatg
//
LOCUS       DASSGX010000505         5296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_305543, whole genome shotgun sequence.
ACCESSION   DASSGX010000505 DASSGX010000000
VERSION     DASSGX010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5296
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305543"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1583)
                     /locus_tag="VFI12_09040"
     CDS             complement(<1..1583)
                     /locus_tag="VFI12_09040"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HET7056596.1"
                     /translation="MIAADPFTTVVLGVYVIAAFIVPTLAPVAVSDDWTYQRSVEYLI
                     NRHEFHILPVAAATQITQLVWGGAFALVFGVTPGVLRVSTLAIVFLSALALRDMFRDL
                     GVSNQRAALGVGLYLFNPIMFAISYSFMSDPHFVAWMTISAWLYLKAEVQKRPELNWW
                     ASGVATIACLERPHGALLPIGVATWYFLSGRLRINRVPVRSLAAIGLIPATAMLLLYT
                     VFSHGLPSQQGLFLDEMRAASPTETRLLIERLAVIEIGYAGFFILPLAIGAFIAIWRA
                     LGDLPPAGVRLTVAATALTAAGMVWLWAQGKLMPYVPHFFGRGGPGSADLRLAKPALW
                     GTGVFEALTVACGAAAVGGLIMCLRAITHRTQRGQAGIGLVICMLGWQAVGVLPQSFL
                     FRNFTISLDRYLLPMLPFVVLVAVWAVDDWNLLPILAWPAIAAVALFSIVGTRDVLVL
                     QNDVWHLAGELNAAGVPNTRLDAGYAWDAYHLWEFSHANSLAPRTPDGPWWTTSYAQA
                     TDSTYVIAAGPIEGYQIISV"
     gene            1828..2664
                     /locus_tag="VFI12_09045"
     CDS             1828..2664
                     /locus_tag="VFI12_09045"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7056597.1"
                     /translation="MNSEYYGEYYEHENSHWWFRWRFDMITRIVDSLPRPDGFRLLDA
                     GCGTGQMTKLLERYGDAVGLEIAPEAIAFARRRGVQNIVQGSIADPPFAAGSFDLVLS
                     LDVIEHVENDAHILTSLFDVLKPGGHLIVTVPAFRSLWSEHDEINQHKRRYRVPQLRG
                     MMEHAGFEVTRITYCKSALFLPVMAMRKGKTWWRRARGHQKTDHPASDLAFYPKPINE
                     ALYRILSAETKVMEHWDLPVGVSILAVAQRPVETGAESPAAVSVDSLIANPETQKVLA
                     AQ"
     gene            2672..3514
                     /locus_tag="VFI12_09050"
     CDS             2672..3514
                     /locus_tag="VFI12_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056598.1"
                     /translation="MNIEQAVPTSDRGSRTGGARDERVFLAIGVVSVLLILGLWAISR
                     DAPSAKQLDSVALVTAGVTGEQGCANFANFWMHDSGLSVLPDAIAGMSNCRLGADGVW
                     FVPAGPSDPRLPADEQLTAQEQEKAAVLSAQLADDLVALEMTLPRSLRESLKANYDPE
                     NLPVFGHTKKGRNDLGAKWARYIRVTQAFLLSPQHAVLADYVGWAMERRQKAVGEFET
                     ACFADPDNAFLWRACKGLRSEFGIANIPLYWELDDPVLIQEYLADRVRSGQPIPVATP
                     ATRS"
     gene            3533..5278
                     /locus_tag="VFI12_09055"
     CDS             3533..5278
                     /locus_tag="VFI12_09055"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HET7056599.1"
                     /translation="MSKRVLIANRGEIAVRIIRACRDLGFPPLAVYGPGDEDAMHVRM
                     ADEAYRIESDRPIPYLDIAALITIARRSGARLVHPGYGFLAENAGFAQACADAGLVFV
                     GPPAAAIATMGDKVAARAAAVAAGVPILPGTTAPVPDAASARQWADGAGYPVVLKAAA
                     GGGGRGFRVAERAEDLDGAFASATSEAERSFGDGRLYAERFLARPRHVEVQIFADSGG
                     TTIAIGDRDCSIQRRHQKLIEESPAPNVPERVREQMAEASIRLAKGVGYVGAGTLEFL
                     VEPDGRFWFLEMNTRIQVEHTVTEEVFGVDLVREQLLAAMGEPLSIPAHAEVRGHAIQ
                     CRINAEDPGNNFAPAPGLVTRFVPPLGPGIRVDTAAESGAVISDRYDSMIAKLIATGP
                     TRDSAIARMRRALDEIVVEGVPSTIPLHRNVMASDAFQRAVLSTSFLVDVADVVPAPV
                     SAQPELPETEPWSERVIEVNGRRFQVRVPGAAPNGTAKASARPKRTARPRSTNGPVGG
                     PRFASPIQGSVLRVHKAVGDAVAAGDPVMVVEAMKMENELRAQRDGVVTEIPVSVGAS
                     VKVGDHLFTIDNSEL"
BASE COUNT          960 a         1666 c         1789 g          881 t
ORIGIN      
        1 accgagatga tctggtagcc ctcgatcgga ccagcggcga tcacgtaggt cgaatcggta
       61 gcttgcgcat aggatgtggt ccaccacggc ccgtccggcg tgcgcggcgc caggctattg
      121 gcgtgactga actcccacaa gtgataggcg tcccaggcat agccggcgtc cagccgcgtg
      181 ttggggacgc cggccgcgtt cagctccccg gccaggtgcc agacgtcgtt ctggagaacc
      241 aggacgtccc gcgtgccgac gatggagaac agcgcgacag ccgctatcgc cggccaggcg
      301 aggatcggca gcagattcca gtcgtcaacc gcccacacgg caaccaggac cacaaacggc
      361 agcatcggca gcagatagcg atccagtgag atcgtgaagt tccggaacag aaatgactgg
      421 ggaagcacgc ccaccgcttg ccagccaagc atacagatga ccaatccgat gcccgcctga
      481 cctctttgtg tccggtgtgt tatcgcgcgc aggcacatga tcagtccgcc gaccgcggca
      541 gccccgcagg cgaccgttaa tgcctcaaac acgcccgttc cccacagcgc cggtttggca
      601 aggcgcaagt cggcggaacc gggcccgccc ctcccgaaga agtgtggcac gtacggcatc
      661 agcttcccct gggcccacag ccagaccatg ccggcagcgg tgagggccgt cgcagcgacg
      721 gtcagacgga ccccggcggg cggaaggtcg ccgagcgcgc gccaaattgc gatgaacgcg
      781 ccaatcgcca gcggaaggat gaagaagccg gcgtatccga tctcgatgac tgccagccgt
      841 tcgatcagga gccgtgtttc cgtggggctg gcggcgcgca tctcatcgag gaacaggccc
      901 tgctgggatg gcaatccatg actgaagacg gtgtagagca ggagcatcgc ggtcgccggg
      961 atcagcccga tcgccgccag agagcggact ggtactcgat tgatccggag ccggccactc
     1021 aggaagtacc aggtcgccac cccaatcgga agcagtgccc cgtgcgggcg ctcgagacat
     1081 gcgattgtcg cgacgcctga tgcccaccag ttgagctcgg ggcgtttctg cacctccgcc
     1141 ttcaggtaga gccacgcgga aatcgtcatc caggccacga aatgcgggtc actcatgaag
     1201 gagtagctga tcgcgaacat gatcgggttg aagagataga gtccgacgcc gagcgcggcc
     1261 cgctgattac ttacgcccag gtcccggaac atgtcccgca gggcgagcgc gctgaggaag
     1321 acgatcgcca gtgtcgagac gcgcagcaca ccgggcgtaa ccccgaacac cagcgcgaat
     1381 gccccgcccc agacgagctg ggtgatttgc gtggccgcgg ccaccgggag aatgtggaac
     1441 tcgtgccggt tgatcaggta ctcgaccgag cgctggtagg tccagtcgtc gctcacagcg
     1501 acgggcgcca gcgtaggcac aatgaaggcc gcgatgacgt ataccccaag gacgacggtc
     1561 gtgaacgggt ccgctgcgat caccagcgtc agcaggtccc agggacgtgg ggctgcttcg
     1621 cgctccagtc ctcgctcctg cgttgagttg ggcgttgtga cggttattgg cggatctcca
     1681 cgcgaatcgc gaatgtggtg cgtttttcgg tcgtcctgat acggagtatt atgatcgccg
     1741 ggttcggggc acagcggcga tgatgccgtg gccggtcgct catatcacac atatacggca
     1801 cacggcacgc aggagtcgca tcacgggatg aattcggaat actacgggga atactacgaa
     1861 cacgagaatt cccactggtg gttccggtgg cggttcgaca tgatcaccag gatcgtcgat
     1921 tcgcttccca ggccagatgg atttcgcttg ctggacgcag ggtgtggcac cgggcagatg
     1981 accaagttgc tcgagcgata cggcgatgcg gtcggtctcg agatcgcgcc ggaagcgatc
     2041 gcatttgccc gccggcgggg tgtccagaac atcgttcagg gctccatcgc cgatccgccg
     2101 tttgcggccg ggtcgttcga tctggtcctc tccctggacg tgatcgaaca tgtcgaaaac
     2161 gacgcgcaca ttctgaccag cctcttcgac gtcctgaagc ccggcggcca cctgatcgtg
     2221 acagtgccgg ccttccggag cctctggtcc gagcacgacg agatcaacca gcacaagcgc
     2281 cggtatcggg tcccgcaact gcgcggaatg atggagcacg ctgggttcga ggtgacgcgg
     2341 atcacctatt gcaagagcgc cctcttcctg ccggtcatgg ccatgcggaa aggcaagacc
     2401 tggtggcgcc gggcgcgggg acaccagaag acggaccacc cggcttccga cctcgccttc
     2461 tatcccaagc cgatcaacga agcgctctac cggatcctgt cggcggagac caaggtgatg
     2521 gagcattggg acctaccggt cggggtttcg atcctggccg tcgcccagcg tccggtcgag
     2581 accggagcag aatctccggc cgctgtttcg gtcgactcgc tgatcgccaa tccggagaca
     2641 cagaaggtgc tcgctgcgca ataatggcgc ggtgaacatc gagcaggcag tgccgacaag
     2701 cgatcgcggg tcccggacag gcggcgcccg ggacgagcgg gtgtttctcg cgatcggcgt
     2761 ggtttccgta ttgctgatcc tcggcctttg ggcaatctct cgcgacgcgc cgtcggcgaa
     2821 gcagctcgat tcagttgcgc tggtcacggc cggggtgacc ggtgagcagg gctgcgccaa
     2881 cttcgccaac ttctggatgc atgactcagg gctgagcgtc ctgcccgacg caatcgccgg
     2941 catgtcaaat tgccggttgg gtgccgacgg cgtctggttc gtgccggccg gaccgtccga
     3001 tccccgcctg cctgccgatg agcaattgac cgcgcaggag caggagaagg ccgcggtgct
     3061 cagcgcgcag ctcgcggacg acttggtggc gctcgagatg acgctgccgc ggtcgctccg
     3121 ggagtcgctc aaggcgaact acgatcccga aaacctgccc gtcttcggtc acacgaagaa
     3181 gggccgcaac gacctcggcg cgaagtgggc gcgctacatc cgcgtcacgc aggcgttctt
     3241 gctttctccg caacatgcgg tgctcgccga ttatgtcggt tgggcaatgg agcggcgcca
     3301 aaaggcagtc ggcgaattcg agaccgcctg ttttgccgac ccggacaatg catttctctg
     3361 gcgggcctgc aagggcctgc gttcagagtt cgggatcgcc aatatcccgc tgtattggga
     3421 gctggacgac ccggtcctga tccaggagta ccttgccgat cgggtgcgat ccggccagcc
     3481 tattccggtc gcgacgccgg ccactcgaag ttagggggag tcaacgaaga ctatgtccaa
     3541 gcgggtgctg atcgcgaatc gtggcgagat cgccgtccgg atcatccgcg cctgtcgcga
     3601 tctcgggttc ccgcctctcg cggtctatgg tcccggcgac gaggatgcga tgcacgtccg
     3661 catggccgac gaggcgtatc gcatcgagtc ggaccggccg atcccgtacc tggacatcgc
     3721 ggccctgatc acgattgccc ggcggtcggg cgcacggctg gttcatccgg gatacggctt
     3781 tctcgcggag aacgccggat ttgcgcaggc gtgcgccgac gctggactcg tctttgtcgg
     3841 cccaccagcc gccgcgatcg cgacgatggg ggacaaggtc gcggcgcgcg cggcggcggt
     3901 cgcggcgggc gtgccaatcc tgcccggaac cactgctccc gtcccggacg ccgcctcggc
     3961 ccggcagtgg gccgacggtg ctggctatcc agtcgtgctc aaggcggcag ccggcggcgg
     4021 cgggcgcggc ttccgcgtcg ccgagcgcgc cgaggatctg gacggcgcat tcgcgagcgc
     4081 gacgagcgag gcagagcgct cattcggcga tggccggctc tatgccgagc gctttttggc
     4141 acgcccacgg catgtcgaag tccagatctt cgcagatagc ggtggaacga cgatcgcgat
     4201 cggcgaccgc gactgctcga tccagcgccg gcatcagaag ttaatcgagg agagcccggc
     4261 tccgaatgtt ccggagcgcg tccgggagca aatggccgaa gcatcgatcc ggctggcgaa
     4321 gggagtcggg tatgtcggcg cgggaacgct cgagttcctg gtcgagccgg atggccgctt
     4381 ctggttcctc gagatgaaca cccggattca ggtcgagcac acggtgaccg aggaagtctt
     4441 cggcgtcgat ctcgttcgcg agcagttgct ggccgcgatg ggcgagccgc tgtcgatccc
     4501 ggcgcacgcg gaggtgcgag gtcatgcaat ccagtgccgg atcaatgccg aggacccggg
     4561 gaacaacttc gcgccggcgc ccgggctggt cacccggttc gtgccaccgc tcggcccggg
     4621 gatccgggtc gataccgcgg ctgagagcgg cgcggtcatc tccgaccggt acgactctat
     4681 gatcgcgaaa ctcatcgcca cgggaccgac ccgtgacagt gcgatcgccc ggatgcgtcg
     4741 cgcgcttgac gagattgtgg tcgagggcgt cccgagcacg attccgctgc accgcaatgt
     4801 gatggcgagc gacgcgttcc aacgtgcggt gctctcgacg tcatttctgg tcgacgtggc
     4861 ggacgtcgtt ccggcgccag tctccgcaca acccgagttg ccggagaccg aaccatggag
     4921 cgagcgggtg atcgaggtca acgggcgccg cttccaggtg cgcgtgcctg gtgccgcgcc
     4981 gaacgggacg gccaaggcgt cggcgcggcc gaagcggaca gctcggccgc gaagcacgaa
     5041 cggaccggtc ggcggaccgc gatttgccag cccgatccag ggttccgtgc tccgggtgca
     5101 caaggcggtc ggagatgcgg ttgcagcagg cgacccggtc atggtcgtcg aggccatgaa
     5161 gatggagaac gagctccgcg cgcaacgcga cggcgtagtg accgagatcc cggtttcagt
     5221 cggggcgagc gttaaggtcg gagaccatct cttcaccatt gacaattcgg aactatagac
     5281 gcgcggtgtc cggaca
//
LOCUS       DASSGX010000506         3552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1615151, whole genome shotgun sequence.
ACCESSION   DASSGX010000506 DASSGX010000000
VERSION     DASSGX010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1615151"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /gene="rpsS"
                     /locus_tag="VFI12_09060"
     CDS             complement(<1..259)
                     /gene="rpsS"
                     /locus_tag="VFI12_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HET7056600.1"
                     /translation="MSRSSKKGPYIDSNLREKIENLNRGSDRRVIRTWARDSTIFPGM
                     VGHTVAIHNGRVHVPIFITEQMVGHKLGEFAPTRTYRGHGKD"
     gene            complement(275..1105)
                     /gene="rplB"
                     /locus_tag="VFI12_09065"
     CDS             complement(275..1105)
                     /gene="rplB"
                     /locus_tag="VFI12_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HET7056601.1"
                     /translation="MPIRTYKPTSPGRRLMSVSTFEEITKTEPEKSLIEPLKKHAGRN
                     NRGRITTRHRGGGHKRFYRIIDFKRNKTGIPAKVAAIEYDPNRSARIALLHYVDGEKR
                     YMLAPVGIGVGDTVISGPGSPIRAGNAMPLRNIPTGTQVHNIELYPGKGGQLCRSAGT
                     SAQLMAKSDKYAQIRMPSGEVRLVHLDCMATLGQVGNVDHENIVIGKAGRNRHRGRRP
                     TVRGAAMNPRDHPHGGGEGKAPAGGQPQTKWGKPAHSRTRNNKRTDQMIVRRRPAKRK
                     "
     gene            complement(1133..1444)
                     /gene="rplW"
                     /locus_tag="VFI12_09070"
     CDS             complement(1133..1444)
                     /gene="rplW"
                     /locus_tag="VFI12_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HET7056602.1"
                     /translation="MPALTDDQVIIRPLITEKNTDLMEHDQYTFVVATEANKIQIREA
                     IERLFNVRVKAVNTMNVKGSSRSRAIRRGKGRIAGQEPSWKKAVVTLFPGQRIEIFQQ
                     V"
     gene            complement(1447..2157)
                     /gene="rplD"
                     /locus_tag="VFI12_09075"
     CDS             complement(1447..2157)
                     /gene="rplD"
                     /locus_tag="VFI12_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HET7056603.1"
                     /translation="MIVDVVDTQGGVVGQTELDDAVWAIEPNIPVMHQAMLRQLANAR
                     LGTRDTKTRGEVRGGGRKPWRQKGTGRARQGSIRSPQWIGGGVVWGPHPRSFTQEMPR
                     KMRRLAVRSALAAKIRDERMTVIDGLAAVEPKTKAMKAILTNLPESRSVLIVTDGKVE
                     PIRRSAGNLSNVWTIDARYLNVRDVLKYDRLLVTREAIPVIEQVWAPAETQREPSDWK
                     QERLAARANGAVAVAQEV"
     gene            complement(2212..2835)
                     /gene="rplC"
                     /locus_tag="VFI12_09080"
     CDS             complement(2212..2835)
                     /gene="rplC"
                     /locus_tag="VFI12_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HET7056604.1"
                     /translation="MQGLIGKKLGMSQVFDDTGLARPVTVLEVGPCVVTQIRTPERDG
                     YSAVQLGFGLDKRLNRSERGHREQSGFMSRTLREVNADNVEELEVGQVINADTFAPGE
                     LVDVTGTSKGRGFQGGVKRHGFRGGPRTHGQSDRLRAPGSIGSSATPGRVFKGLRMAG
                     RMGNDRVTVQNLKVLRVDPERNLLLVQGSVPGPNEGLVLVRRAVKSG"
     gene            complement(2942..3262)
                     /gene="rpsJ"
                     /locus_tag="VFI12_09085"
     CDS             complement(2942..3262)
                     /gene="rpsJ"
                     /locus_tag="VFI12_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008933160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HET7056605.1"
                     /translation="MVARKPSQRIRIRLKAYDHKVLDQSASKIVETAESTGAKVAGPV
                     PLPTRIEKFCVIRSPFIDKDSQEQFEIRTHKRLIDVLEPSGSTIRSLMRLNLPAGVDI
                     EIKL"
     gene            complement(3306..>3552)
                     /gene="tuf"
                     /locus_tag="VFI12_09090"
     CDS             complement(3306..>3552)
                     /gene="tuf"
                     /locus_tag="VFI12_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499102.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET7056606.1"
                     /translation="KEEGGRHTPFFPGYRPQFYIRTTDVTGAITLPENTEMVMPGDNV
                     QMGVELITPVAIEEGLRFAIREGGRTVGAGVVTSIVQ"
BASE COUNT          519 a         1253 c         1075 g          705 t
ORIGIN      
        1 cgtctttgcc gtggccgcga taggtgcgcg tcggagcgaa ctcgccgagc ttgtgtccga
       61 ccatttgctc ggtgatgaag atcggaacgt gcaccctgcc gttgtgaatc gccaccgtgt
      121 gcccgaccat gccgggaaag atcgtcgaat cgcgcgccca ggtccggatc accctccggt
      181 cgctgccgcg attgaggttt tcgatctttt cccgcagatt ggaatcgatg tacggtccct
      241 ttttggacga cctcgacacg tcactcctcc tcgattactt ccgtttcgca ggccggcgac
      301 ggacgatcat ctgatcggtc cgcttgttgt tgcgggttcg tgaatgcgcc ggcttgcccc
      361 atttcgtctg cggctggcca cccgccggcg ccttcccctc gccaccgccg tgcggatggt
      421 cacgggggtt catcgccgcg ccgcgcaccg tgggccgccg cccgcgatgc cgattgcgcc
      481 cggctttgcc gatcacgatg ttctcgtgat cgacgttgcc gacctggccc agcgtcgcca
      541 tgcaatcgag gtgcacaagc cgcacctcgc ccgacggcat ccggatctgc gcgtatttgt
      601 ccgacttcgc catcagttgg gccgaggtgc cggcggagcg gcagagctgc cctcctttgc
      661 cggggtagag ctcgatgttg tggacctgtg tgccggtcgg aatattccgc agcggcatcg
      721 cgttgccggc gcggataggc gagcccgggc cggagatgac cgtgtcgccc acgccgatgc
      781 cgactggcgc cagcatgtag cgcttttcgc cgtcgacgta gtgcagcagc gcgatgcgcg
      841 ccgaccggtt ggggtcgtac tcgatcgccg ccaccttggc gggaatcccg gtcttgttgc
      901 gcttgaagtc gatgatgcgg tagaagcgct tgtggccgcc gccccggtgg cgcgtcgtga
      961 tgcgaccccg gttattgcgg cccgcgtgct tcttcagcgg ctcgatcaag ctcttttccg
     1021 gctcggtctt ggtgatctcc tcgaacgtgc tgacgctcat caggcgccgg cccggcgagg
     1081 ttggtttgta tgtgcgaatg ggcatcctac tatccttccg tcgccgcgcc cgttacacct
     1141 gctggaaaat ctcgatccgc tgcccaggga agagcgtcac gaccgccttc ttccagctcg
     1201 gctcctgccc cgcgatccgc cctttcccgc gccggatggc gcgagagcgc gaactgccct
     1261 tcacgttcat cgtgttcacg gccttgacgc ggacgttgaa caatcgctcg atcgcctcgc
     1321 ggatctgaat cttgtttgcc tcggtcgcca cgacgaacgt gtactgatcg tgctccatca
     1381 gatcggtgtt cttctcagtg atcaacggcc ggatgatgac ctgatcgtct gtcagcgctg
     1441 gcatactcag acctcctgcg ccacggcgac ggcgccattc gcccgggcgg ccagccgctc
     1501 ctgcttccag tcgctcggct cacgctgcgt ctctgccggc gcccagacct gctcgatcac
     1561 cgggatcgcc tcgcgagtga cgagcagccg gtcgtacttc aggacatcgc gcacattgag
     1621 ataccgcgcg tcgatggtcc agacgttgga cagattgccc gccgaccgcc gaattggctc
     1681 taccttgccg tcggtcacga tcaacacgga ccggctctcc ggcaggttcg tcaggatcgc
     1741 tttcatcgcc ttcgtcttcg gctcgaccgc ggcgagaccg tcgatgaccg tcatccgctc
     1801 gtcgcggatt ttcgccgcca gcgccgagcg caccgccagg cgccgcatct tgcgcggcat
     1861 ctcctgggtg aacgaccgcg gatgcggacc ccagacgacg ccgccgccga tccactgcgg
     1921 cgaacggatc gagccctgcc gcgcacggcc ggtgcccttc tggcgccacg gcttgcggcc
     1981 gccgccgcgc acctcgccgc gggtcttggt atcccgcgtc ccgagccgcg cgttggcgag
     2041 ctggcgcaac atcgcctgat gcatcaccgg aatattgggc tcgatcgccc agaccgcatc
     2101 atccaattcg gtctggccga ccacgccacc ttgcgtatca accacatcaa caatcatgtc
     2161 tctctccgga gccagagccg ggggtgccgg cggccacgaa cgctcggggc gctaccccga
     2221 cttgacggca cgtcgcacga gcaccagacc ttcgttcggt cccggcaccg agccctgcac
     2281 cagcagcaga ttccgctcgg gatcgacgcg gagcaccttc aggttctgga cggtgacccg
     2341 atcgttcccc atccggcctg ccatgcgcaa ccccttgaac acgcggcctg gcgtggcgct
     2401 agagccaatc gaccccggtg cgcgcagccg atccgactgg ccgtgcgtgc gcgggccgcc
     2461 acggaacccg tggcgcttga ctccgccctg gaacccgcgc cccttcgagg tgccggtgac
     2521 atcgaccagc tcgccgggcg cgaaggtgtc ggcgttgatc acctggccga cctcgagctc
     2581 ttcgacgttg tcggcattca cttcgcgcag cgtccgcgac atgaagccgc tctgctcacg
     2641 atggccgcgc tccgagcgat tcaaccgctt gtcgagaccg aacccaagct ggaccgcgct
     2701 gtagccgtcc cgctccggag tcctgatctg cgtcactaca caagggccga cctccagcac
     2761 cgtcaccggc cgcgccagcc ccgtgtcatc aaatacctgg gacatgccca gtttctttcc
     2821 gatgagccct tggatcattg gctctctcct gtcctccagg aagcggcgca gcctgcctcg
     2881 gccgcgtccg cttacagagc gtgtccagcg ctccgggtca ccttcggtga cccgggctct
     2941 gctacagctt gatttcgata tccacgccgg cgggcagatt cagccgcatc agcgaccgga
     3001 tcgtgcttcc gctcggctcc aacacgtcga tcaaccgctt gtgcgtgcga atctcgaact
     3061 gctcctgcga atccttgtcg ataaatggcg agcggatcac acagaacttc tcgatccgcg
     3121 tcgggagggg caccgggccg gccactttgg cgcccgtcga ctccgccgtc tccacaatct
     3181 tgctcgccga ttgatcgagc actttgtggt cataggcctt caggcggatg cgaatccgct
     3241 gactgggctt gcgtgcgacc atcggttttt cgctcctgcg cgctgtggcg tcctgcatta
     3301 cggctctact gaacgatgct ggtgacgacg ccggcgccga cggtgcggcc gccctcgcgg
     3361 atggcgaagc gcagcccctc ttcgatggcg accggggtga tcagctccac tcccatctgc
     3421 acgttgtcgc ccggcatcac catctcggtg ttctccggca aggtgatggc cccggtcacg
     3481 tccgtggtgc ggatgtagaa ctgcggccgg tagccgggga agaacggcgt gtgccggccc
     3541 ccctcctctt tc
//
LOCUS       DASSGX010000507         6393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_853738, whole genome shotgun sequence.
ACCESSION   DASSGX010000507 DASSGX010000000
VERSION     DASSGX010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6393
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_853738"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VFI12_09095"
     CDS             <1..180
                     /locus_tag="VFI12_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056607.1"
                     /translation="PYRYLEIRGTVVEVQPDPDKAFIDRMAQKYLGEEKYPWSRPGDE
                     RVIVVVEPDRTTSMG"
     gene            352..672
                     /locus_tag="VFI12_09100"
     CDS             352..672
                     /locus_tag="VFI12_09100"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET7056608.1"
                     /translation="MTAVNIPGLLTVADAATELSRSTEQVRRYLREGRLRGRRLGGQW
                     FIEEDALVAFREALNDQSSYLDKLRPAAQIMALDDVIGIGHGGGSNIAEGKDSYRRSS
                     WWRR"
     gene            693..1094
                     /locus_tag="VFI12_09105"
     CDS             693..1094
                     /locus_tag="VFI12_09105"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HET7056609.1"
                     /translation="MASDDRPVAFVDSSAIVALVDEDDAAHSAAVDAYRELSTSGYRL
                     FTTDHVVVEAYDLLLNGVGPVVATAWLRDQRLPVYCTDEADLAAAQTILAARPADAPV
                     SFTDALSLAVMERLGVADAFAVDPAFLNALS"
     gene            1368..2024
                     /locus_tag="VFI12_09110"
     CDS             1368..2024
                     /locus_tag="VFI12_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056610.1"
                     /translation="MATRPYTPVLDPWSSMGGRLGAEPGASDAFVPPVSSPDARREGG
                     PPAWFLGLVGILLIGVVGFAALALVIRPVVSDRVGSAASDAISISLAHATVVPDVSSG
                     TVVVTEQEISQAIRANRDELQPLENLRVQIRRTGIEATFSVYGVPGTLTGSVKVQKGK
                     IVIVDPKLTGAVGRMIEVDRIAHEAEQAINDLLARNNLRATAVTLSDDTMTISTAPRT
                     "
     gene            complement(2383..3588)
                     /locus_tag="VFI12_09115"
     CDS             complement(2383..3588)
                     /locus_tag="VFI12_09115"
                     /inference="COORDINATES: protein motif:HMM:NF015963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HET7056611.1"
                     /translation="MAEWRNWAGNVLATPARIERPGSIEAVQELVAGAVRAGQKVRVA
                     GAGHSFTPICATDGLLLDLCDLSGVVEIDTDEQTATVFGGTRIHQIGAPLFEAGLAVA
                     NQGDIDVQAIAGAIGTGTHGTGRRFGSFSSTLIACQLVTATGELLTIDESDMETLRAA
                     RVSLGTLGVMVSVTLQLVPAYKLQRRSWPIAWDDAIAQWPEIEANARNAEFWWVPPLD
                     TSVFKVFIDTDEEPTGTPPALTYPPGTIERYLPPDGVDWAWRVFPAIREHRFVEMEYT
                     FPIERGLDALAAVRDVMLSRHPEVKWAVEFRTHAGEDAFLSVTQGHDSVTISVHDAAD
                     NPHWDFFREAERVFRRFDGRPHWGKLNFLETAELQSLFPLLDRFAAIRCRFDPNGVFL
                     NDYLRPILA"
     gene            complement(3642..4439)
                     /locus_tag="VFI12_09120"
     CDS             complement(3642..4439)
                     /locus_tag="VFI12_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056612.1"
                     /translation="MTMHLLSEAVFDVTIWRDDYEQSVTELTAAGPALRPMAESVLAH
                     PETGWWFGPMLRDRQLWHVSGIQTEFSAAREPFVPNRGPTSWELYAQKPAWGLQTSTD
                     TTRSGFSSLLVGNSLCAYDLGPIDYPLERLRLTVSPSARVYTVDGPEAWRRLCLTYPV
                     RYGNGAIAPSQESFERLHADFLHKPWYTRGHIIPDFASVSSDFDAVHITLSGLLTGSH
                     VRLEGPEGWTWLWGFDAEVTFWLRWVFENAERLPDLTEPILTPPQLR"
     gene            complement(4667..5482)
                     /locus_tag="VFI12_09125"
     CDS             complement(4667..5482)
                     /locus_tag="VFI12_09125"
                     /inference="COORDINATES: protein motif:HMM:NF010068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HET7056613.1"
                     /translation="MADTAPPRVIATDVGYSVGSATLLQGVSLTVMPGEVLVLVGPNG
                     AGKSTLTGVLSGDLTPTSGEVLLDGRSISSYRPNELAKKRSVLPQQSLLQFAFTVREV
                     VEMGRTPHDDTADELRAIVDRALERTEMIPFADRVYPSLSGGEKARAQLGRVIAQDAP
                     LLLLDEPTASLDIRHQQHVMHLAQDVAAAGGSVVAVIHDLNLAASTAHRIALLHRGRI
                     VADGAPWVVMEEKLLSEVYQCPIAVSKHPILHCPLILPLGRYGTTERYVSTTH"
     gene            complement(5483..>6393)
                     /locus_tag="VFI12_09130"
     CDS             complement(5483..>6393)
                     /locus_tag="VFI12_09130"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HET7056614.1"
                     /translation="LHHAAIGTDIVFTRQQDAVVWAIRIPRVLLATIVGATLAISGAV
                     LQGIFRNPLADPSIVGVSSGAAFGAVTAIVVGATPFGLMTTPIAAFAGGIVTTLMIWS
                     LSRRNGRVDTVTLILVGIAINSMIGAAMGMLNYMADDQQLRSVVFWSMGSLGGATWQS
                     NLAILPLVALGFVVLPRKARDLNLLVLGEREARHLGVDVDRTRFLLILVVALTTAAAV
                     AFAGVIGFVGLIVPHLVRLVAGPDHRVLLPASALAGASLLVLTDLLARTIVSPAELPI
                     GIVTAMLGAPFFLFLLLKTRWQHGVM"
BASE COUNT         1152 a         2038 c         2157 g         1046 t
ORIGIN      
        1 ccgtatcgct acctcgagat tcgcggcact gtcgtcgagg tccagccgga tccggataaa
       61 gccttcatcg accggatggc gcagaagtac ctgggcgagg agaagtaccc gtggagccgc
      121 ccgggcgacg agcgcgtgat cgtcgtcgtc gagccggatc gcaccacgtc tatgggctaa
      181 cgcccgttcc cgatctcgac ctacggcccg ctcgttctgc gagcgggccg ttttgcgtgt
      241 acagacgggc cgccatcggt cctgatcccg cggcagctct tgacaggacc atgttgtcgt
      301 gtgctacgct gttgctgcga gctgtacgta cgcaagggcg agagttggca aatgacagca
      361 gtgaacattc ccggattgct cacggtggcg gacgcggcaa ccgaattgag ccggtcgact
      421 gagcaggtgc gccgctatct gcgcgaagga cggctgcgcg ggcgacgact cggtggccaa
      481 tggttcatcg aggaggacgc gctggtggcc ttccgcgaag cgctcaatga ccagagttcg
      541 tacctcgaca agctgcgtcc ggctgcgcag atcatggcgc tcgatgacgt gatcggaatc
      601 ggtcacgggg gcggcagcaa tatcgccgaa ggcaaggatt cgtatcgtcg gtcgtcctgg
      661 tggcgacggt agggcagagg gagcatccgc agatggcatc agatgaccgg ccggtcgcgt
      721 ttgtcgattc ctccgcgatc gtcgctcttg tcgatgaaga cgacgccgcc cattcggccg
      781 cggtggacgc ctaccgggag ctcagcacgt ccgggtaccg gctattcacg accgatcacg
      841 ttgttgttga ggcgtacgac ttgctgctga acggggttgg accggtcgtt gcgacggcct
      901 ggctgcggga tcagcgctta cccgtctact gcacggacga ggcggatctc gccgcggcgc
      961 agacgattct tgcggcgcgc ccggccgacg ccccggtctc cttcaccgat gcgctcagtc
     1021 tggctgtaat ggagcggctg ggtgtggccg atgcctttgc ggtcgacccc gcctttctga
     1081 atgcgctgag ctaggccggc gtgcgcggga ttccaatgcg acgtaacctg acgggcaatg
     1141 agaaaccgga ggagcggcca cgcttctgcc cgaactgtgg ggccgcgaat acaacgttcg
     1201 gcctgcgctg cacgaattgc gggcacctgt atgccgacaa gccgccaatc gagagttatt
     1261 gggatcgccc gaaccctccg tcagctccaa tctccgagta cagcggcgag gacgcatctc
     1321 cacctccgga ttcggatgag gaccagagtg ataccgcggc ggctgcgatg gcaacgcggc
     1381 cgtacacgcc ggtcctcgat ccctggtcgt caatgggtgg ccgcctcggc gccgagcccg
     1441 gggcgtccga tgccttcgta ccgccggttt ccagcccgga tgcgcgccgc gagggtgggc
     1501 cgccggcctg gttcctcggg ctggtgggaa tcctgctgat cggggtagtc ggcttcgcgg
     1561 cgctggccct tgtcatccgg ccggtcgtgt cggatcgggt aggatcggcc gccagcgacg
     1621 cgatcagcat cagtttggcc catgcgacgg tcgtgccgga tgtcagctcc ggaactgtcg
     1681 tggtgaccga gcaggagatc agccaggcga tccgcgcgaa ccgggatgag ctgcaaccgc
     1741 tcgagaatct ccgggtccaa atccgccgaa ccggcatcga ggcgaccttc tcggtctacg
     1801 gcgtgcccgg gacgttgacc ggctcggtaa aggtccaaaa gggcaagatc gtgatcgtcg
     1861 atcccaagct gaccggcgcc gtcggccgca tgatcgaggt cgatcgcatt gctcatgaag
     1921 ctgaacaggc catcaacgat ctcctcgccc gcaacaacct ccgcgccact gccgtgaccc
     1981 tctcggacga cacgatgacg atctcgacgg caccgagaac gtaggcgagt agacgaggtt
     2041 gagggtggag ggaacgaagg taggggcaga tggcatctgc ccatggtcgc ccgagggtcc
     2101 ggtggcgtgg gaacgtgcgt gcccggggtc gcccgggcgt aaacacccgg gctaagacct
     2161 gaagccggct gaagccggct cgggatgcgc gtgcctggat gtggcgcgcc tcgaccgtag
     2221 cgcggtgcgg cagccccgcg cccggacccc gtcccgatga ggtcgttccg gggaccctag
     2281 cccggccacg gtccgccgcc atcgaccgtc cgtgggatga ccgatgccgg ccacccgtgg
     2341 ccggcgttac gctctcccaa aacccaaaac ccaaatcccc cgtcaggcca ggatcgggcg
     2401 cagatagtcg ttcagaaaga cgccattggg gtcgaagcgg cagcggatgg cggcaaagcg
     2461 gtcgagcagg gggaagaggg actggagctc ggccgtctcg aggaagttga gcttgcccca
     2521 gtgcgggcgg ccgtcgaaac ggcgaaatac gcgctccgcc tcccggaaga agtcccagtg
     2581 cgggttgtcc gccgcgtcgt ggacgctgat cgtgactgag tcgtgaccct gcgttacgct
     2641 cagaaaggcg tcctcgccgg catgcgtccg gaactcaacc gcccatttca cctcgggatg
     2701 ccgggacagc atcacgtcgc gaacagccgc cagcgcatcc agcccgcgtt cgatcgggaa
     2761 ggtgtactcc atttcgacga agcggtgctc gcggatcgcc ggaaacaccc gccacgccca
     2821 atcgacgccg tccggcggca ggtaacgctc gatcgtcccg ggtggatagg tcagcgccgg
     2881 gggagtgccg gtcggttcct catccgtgtc gatgaagacc ttgaagacgc tcgtgtccag
     2941 cggcggaacc caccagaact cggcgttccg cgcattcgcc tcgatctccg gccactgcgc
     3001 gatcgcgtcg tcccacgcaa tgggccagct ccgccgttgc agcttgtagg cggggacgag
     3061 ctggagcgtg accgagacca tcacgccgag cgtcccgagc gagacccgcg ccgcccgcag
     3121 cgtttccatg tccgattcat cgatggtcag cagctcgccg gtcgcggtca cgagctggca
     3181 ggcgatcagc gtcgaggaaa aggaaccgaa ccggcggccg gtgccgtggg taccggtccc
     3241 gatcgcgccg gcgatggcct ggacatcgat gtcgccctgg ttggcgacgg ccagtccggc
     3301 ttcgaacagg ggtgcgccga tctggtgaat ccgggtgccg ccgaaaacgg tcgcggtctg
     3361 ctcgtcggta tcaatctcga ccacgccgga cagatcgcag agatcaagga gcaggccgtc
     3421 ggtcgcgcaa atcggcgtaa aggagtgccc ggcgccggca acccgcacct tctgccccgc
     3481 ccgcaccgca cccgcgacca gctcctggac cgcttcgatc gatcccggcc gctcgatccg
     3541 cgccggcgtc gcgaggacat tcccggccca attccgccac tcagccatat gccacccctc
     3601 tcgcactgct ttgagttacg gcagcatacg gcactcgcgt gttagcgaag ctgcggcgga
     3661 gtcagaatgg gttcggtcaa gtcgggcagc cgttcggcgt tctcgaatac ccaacggagc
     3721 cagaaagtta cttccgcgtc aaagccccag agccatgtcc agccttccgg cccttctaat
     3781 cgtacatgcg agccagtcag gagaccgctg agcgtaatat ggacggcatc gaagtcgctg
     3841 gagactgagg cgaagtcggg gatgatgtgc ccccgggtgt accacggctt gtgcagaaaa
     3901 tccgcgtgga gccgctcaaa cgattcctga ctgggtgcga tcgcgccgtt cccgtatcta
     3961 accgggtagg tgagacaaag gcggcgccac gcctcggggc cgtccaccgt atagacgcgc
     4021 gcggatggcg acactgtgag ccgaagtcgt tcgagcgggt aatcgatcgg tcctaagtca
     4081 taggcgcaca ggctgttccc aacgagcaga gagctgaagc cggatcgagt tgtgtctgtt
     4141 gaagtctgga ggccccatgc cggcttttgg gcgtacagct cccaagaagt gggcccgcgg
     4201 ttggggacga acggctcccg ggcggccgag aactctgttt ggatcccgct tacgtgccat
     4261 agctggcggt cccgcagcat tggcccgaac caccacccgg tttctggatg ggcaagaacc
     4321 gattcggcca tcggtcgcag ggccggtccg gcggcggtca gctctgtaac ggactgctcg
     4381 tagtcgtcgc gccaaatagt gacatcgaag acggcctcac tgagcaggtg catcgtcatg
     4441 ataggttcga caatttcggc aacagttaac ccagcccagc tcgccctaac cagaaatgcg
     4501 cagccgagtg gtgcctttgt cagggcatcc gcccgtgacc gaaccatatt cacatccact
     4561 acatgctctc ccctaaccgc tgagccctgc ccggaaactc attcccagcg tagcccaaag
     4621 tggtgagccg gctttcggtt ccgacagacg gacttgagtc cccaacttaa tgggtggtcg
     4681 aaacgtagcg ttccgtcgtg ccgtagcggc cgagggggag gatcaggggg cagtggagga
     4741 tggggtgctt gctgacggcg atcgggcact ggtagacctc gctcagcagc ttctcttcca
     4801 tcaccaccca cggcgcgccg tcggccacga tccggccccg atgaagcagg gcaatccgat
     4861 gggcggtgct ggcggccagg ttgaggtcgt ggatgacggc gacgacagac ccccccgccg
     4921 ctgcgacgtc ctgcgcgagg tgcatcacgt gttgctgatg gcggatatcg agcgaggcag
     4981 tcggttcgtc gagcaggagg agtggggcgt cttgcgcaat gacccggccg agctgggcgc
     5041 gagccttctc gccgccggag agggacgggt agactcggtc agcgaacgga atcatttcag
     5101 tccgctcgag ggcgcggtcg acgatggcgc ggagctcgtc tgccgtgtcg tcgtgcgggg
     5161 tccggcccat ctcgaccact tcgcggacgg taaaggcgaa ctggagcaac gattgttggg
     5221 gcagcaccga tcgcttcttc gccagctcat tcggccggta cgagctgatc gatcggccgt
     5281 cgagcagcac ctcgcctgag gtcggggtga gatcgccgga aagcacgccg gttagtgtcg
     5341 atttgccggc cccattcggc ccgaccagca ccagcacctc gcccggcatg accgtgagcg
     5401 agacgccctg gagcagtgtc gccgagccaa ctgaatagcc aacatcggtc gcgatcaccc
     5461 ggggaggtgc ggtatcggcc atctacatga cgccgtgctg ccagcgggtc ttgagcagca
     5521 ggaagaggaa gaagggcgcg ccgagcatgg cggtcacgat cccgatcggc agctcggccg
     5581 gggagacgat cgtccgcgcc agcaggtcgg tcaggaccag gagcgatgcc ccggcaagcg
     5641 cgctggccgg cagcagcacc cggtgatccg ggccggcgac aagccggacc aggtgcggca
     5701 cgatgagccc gacgaagccg atcacgccgg cgaaggcgac cgccgccgcc gtggtcagcg
     5761 cgacgaccag gatcagcagg aatcgggtcc ggtcgacatc gacgccgagg tgccgcgcct
     5821 cgcgctcacc gagcaccaga agattaaggt cccgggcctt ccgcggcagg acgacgaagc
     5881 cgagcgcgac gagcggcaga atcgcgaggt tgctctgcca ggtcgcgccg ccgaggctgc
     5941 ccatcgacca gaaaacgact gagcggagct gctggtcgtc ggccatgtag ttgagcatgc
     6001 ccatcgccgc accgatcatc gaattgatcg cgatcccgac cagaatcagc gttaccgtgt
     6061 ctacccggcc gttgcggcgc gagaggctcc agatcatgag cgtggtgacg atgccgccgg
     6121 cgaaagcggc gatcggtgtc gtcatcaagc cgaacggcgt cgcgccgacc acgatggccg
     6181 tgaccgcgcc gaaggccgcg ccgctcgaaa ccccgacgat gctcgggtcg gccagcgggt
     6241 tgcggaagat gccttgcagc accgcgccgg agatggccag ggtcgcgccg acgatcgtcg
     6301 ccagcaggac gcgcggaatc cggatcgccc aaaccaccgc gtcctgctgg cgggtgaaga
     6361 cgatatccgt gccgatggcg gcgtggtgaa gga
//
LOCUS       DASSGX010000508         6524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_417646, whole genome shotgun sequence.
ACCESSION   DASSGX010000508 DASSGX010000000
VERSION     DASSGX010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6524
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417646"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..354)
                     /locus_tag="VFI12_09135"
     CDS             complement(79..354)
                     /locus_tag="VFI12_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056615.1"
                     /translation="MSTNKKQSRQSVNGGNPGDGAGRTEDTQSQHSGVWPASGPHPDP
                     PDVPYQGMASFGQGERGAEGYQDSGQSELQTMPPDGETSGSETGSGA"
     gene            complement(388..1710)
                     /locus_tag="VFI12_09140"
     CDS             complement(388..1710)
                     /locus_tag="VFI12_09140"
                     /inference="COORDINATES: protein motif:HMM:NF016994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HET7056616.1"
                     /translation="MQRLGTAPLVDDAALGRQILTFSSPLEDSEDLDPLLDRIGDARF
                     VLLGEASHGTHEYYLWRARISRRLIEEKGFDFIAVEGDWPDCYRVNQYVRGELPDQTA
                     EEVLRAFNRWPTWMWANWEIVALTEWMQAHNDGKPSAEQVGFYGLDVYSLWESLAEVT
                     NYLNEHHPESVQAAYEAFRCFEPYAEDPQEYARGTLFVPDDCRDEVQKLLQTVRGRAA
                     DLATGDAASLNAIMNAEAVQGAEAYYRAMVAGGADSWNLRDRHMTNTLNRLIQFHGPD
                     SKAIVWEHNTHIGDARYTDMARDGMFNVGQVVREEHGDDGVVLAGFGSYQGTVIAGRS
                     WDAPMEVMDVPPAQTGSWEQALHDLDAEDRLLLFHPPATAGTELAVPRGHRAIGVVYR
                     PEYERGNYVPTVLPLRYDAFLYLDRTEALHPLHIEPKAGPPELYPWNV"
     gene            2147..3220
                     /locus_tag="VFI12_09145"
     CDS             2147..3220
                     /locus_tag="VFI12_09145"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET7056617.1"
                     /translation="MRERCPVAYSEFLAWSIFRHDDIETILLDPETYSSASRRLAVPN
                     GMDAPEHTAYRAAQEPFFSSDQMATFDSQCRKVARELVGPLVARSTVEIVADFADPFS
                     MRSLCAFLGWPEENWGYLLGWTHGNLQAAYARDRDAGKTLAEEFTHYVMEQIDARRTD
                     GRSESGDDVTSRLMRRTVNGRRMTDEELISALRNWTAGHGTVVAGLSNLLLYLARHPQ
                     FQDHLRSEPGLIPAAVDEVLRVDGPLVSNRRTTTREVEIGGRVIDAGQNLTLMWLAAD
                     RDPREFDEPTTVNLDRDQAGNFLFGAGIHYCVGAPMARLELRIALEELLSHTRELALA
                     GNPNPPRSTYPGNGVEVLQMHLR"
     gene            3426..4268
                     /locus_tag="VFI12_09150"
     CDS             3426..4268
                     /locus_tag="VFI12_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056618.1"
                     /translation="MGIAVAQQATPDNAAFSGMPELTVTITDDAVQVDKTEIPDGYVL
                     LNVVNNSKDNNSAGVLGPGPGRTMDDLKAAAATPEASDEIPPFLYEATILGGPGDIDP
                     GKSAQAVINVPAGDWAVFAEGSQPPVFISAKQTADSSTRPPTADTKIELGDFYFNGFS
                     EGVKPGPQVWEVTNTGKQIHMMVVGKVPDGTTEQQVIDTFTAQETGTPVAGALQESDL
                     QPAGTGVLLISSGQTMYVTADLEAGTYVALCFVTDPQTHKLHAMEGMVSVFQVGQGTA
                     TPTS"
     gene            4379..5092
                     /locus_tag="VFI12_09155"
     CDS             4379..5092
                     /locus_tag="VFI12_09155"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HET7056619.1"
                     /translation="MSVLLVRIDKHQIDVEVARKEIRHLYIRVDRIDGRVRVSAPRGM
                     SDDAIRDAVRRNHDWIARRRESATSRESPARLELVTGDLVPVLGDQVHLYVRESNTGR
                     RSVHLDAEGRLAMHIAPGSTRETRATLLDDWYRRQLRDRIPVLIKAWEPEMGVSVAEW
                     GVKRMRSRWGSCNPRARRIWLSLELARRPVACLEYVVVHEMVHLLERGHNRRFYAFMD
                     QFLPDWRLARAELRSSPPD"
     gene            5422..>6524
                     /locus_tag="VFI12_09160"
     CDS             5422..>6524
                     /locus_tag="VFI12_09160"
                     /inference="COORDINATES: protein motif:HMM:NF017722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfotransferase family protein"
                     /protein_id="HET7056620.1"
                     /translation="MRVFPVPDSMTASPGTQLSFRGPGLATVQPIEVIGSESGVHPGV
                     EVAHSDGNGMSWIPDFGFRPGEAVTVSTRLDIAGTRKGDFSFIVSTPRPLAAHPRRNK
                     KPEEKQVHHFRSRPGLAPQVFDVTQHGESLAPGYLFDAPKEGAGRNGALIMDNSGEPV
                     WFFPVEAPVDQILDFRVQELDGEPVLTWWQGVVVRGHGFGHWVIRDGSYEEIANIRIG
                     NGYAGGDLHELVLTEERTALVGTYSTIAWDLTSIGGKKDDSIIDCIVQEIDIATGAVM
                     FEWHSLDHVALDETYDKTDEGDEEDALDHFHFNAITLDSDGNLVISARNTWAAYTIDR
                     ETGDVIWRLNGKASDFVVGDGADMAYQHDVRHWP"
BASE COUNT         1184 a         2023 c         2100 g         1217 t
ORIGIN      
        1 cgtcacgaat gcgaagacat cgtccgccgg cgcattgatg gtgcgagtgc gttcatattc
       61 cgccacgggg tcctcctctc aggctcctga tccagtctcg ctgccggatg tttcgccgtc
      121 cggcggcatg gtctggagct ccgattgacc gctgtcctgg taaccctcgg ctcccctctc
      181 accttgtccg aatgacgcca tgccctggta cggcacatcc ggggggtcag gatgtggtcc
      241 cgacgccggc cagacgccgg agtgctgcga ctgcgtgtcc tccgtgcgcc cggcgccatc
      301 gccgggattt ccgccattaa cggactggcg ggattgcttc ttgttggtgc tcatgtcttg
      361 cctccaatcg tcgtgaacga tgacgagcta gacgttccac gggtagagtt cgggcggccc
      421 ggccttcggc tcgatatgga gcggatgcag cgcctcggtc cggtcgaggt agaggaaggc
      481 gtcgtaccgg agtggcagca cggtcggaac gtagttgccc ctttcgtact cggggcgata
      541 gacgacgccg atcgctcggt ggcctcgcgg caccgcgagt tccgtgccag cggtcgccgg
      601 cggatggaag agcagcaggc gatcctcggc gtcaaggtcg tgcagcgctt gctcccaact
      661 cccggtctgg gctggcggca cgtccatcac ctccatcggc gcgtcccagc tccggccggc
      721 gataaccgtt ccctggtagg agccaaagcc ggcgagaacg acaccgtcgt cgccgtgctc
      781 ttcgcggacg acctggccga cgttgaacat gccatcgcgg gccatgtccg tgtaccgggc
      841 atcgccgatg tgggtgttgt gctcccagac aatcgccttg ctgtccggcc cgtggaactg
      901 gatcagccgg ttcagcgtgt tggtcatgtg gcgatcgcgg aggttccacg agtccgcgcc
      961 gccggccacc attgcgcggt agtacgcctc ggcgccctgg actgcttctg cattcatgat
     1021 cgcattgagg ctggctgcat cgccggttgc gagatcggcc gcccggccgc gcacagtttg
     1081 caggagcttc tgcacctcgt cccggcagtc gtccgggacg aacagggtgc cgcgggcgta
     1141 ctcctgcgga tcttcggcat acggttcgaa gcagcggaac gcctcgtagg ctgcctggac
     1201 cgactccggg tgatgctcgt tgagataatt ggtcacctcg gcgagggatt cccagaggga
     1261 gtagacgtcg agcccgtaga atccgacttg ttccgcgctc ggcttgccgt cattgtgggc
     1321 ttgcatccac tcggtcagcg cgacgatttc ccagttcgcc cacatccagg tcggccagcg
     1381 attgaaagcg cgcaggacct cctcggccgt ttggtccggg agctcacccc ggacgtactg
     1441 gttgactcgg tagcaatcgg gccagtcccc ctcgacggcg atgaagtcga accctttctc
     1501 ctcgatcagc cggcggctga tccgggcccg ccagaggtag tactcgtgcg tgccatgtga
     1561 ggcttcgccc aagagcacga aacgggcatc gccgattcgg tcgaggagcg gatcgaggtc
     1621 ttcgctgtcc tcaaggggag aggaaaacgt caggatttgc cggccgagcg cagcatcgtc
     1681 gacgagcggg gccgttccga ggcgttgcat ggagcctcct tccagttgtt tcggggcgga
     1741 cgtctttggc ccggattcga ctccggcccg cgcaacgtcc gtcgcgctca atcccatgct
     1801 aggtcgctag ccggcaccat ccaaccgaca aagcgacgcc ggactacgga ccgtccagag
     1861 tgtcctcgag gacgagatgg aggtcgtcaa gaaatcagaa tcagccaccc cgggagcgct
     1921 ctggcatgaa ctccgcggtc ccagtctggg atggagatca ggcactcaat gttcggtcgg
     1981 cgacaggtag gggctgatat ctcgcgattt gattggtacg gtgaagttgg acgtattgaa
     2041 acctccagag agagtgaagg gagctcacgt gctactccag gccgcgccag attgggatcc
     2101 tcgtgaccct gcgattcaga gtgaccagat tcgtgcatat gacgatatgc gcgagcgatg
     2161 ccctgttgcg tacagcgagt ttcttgcctg gtccatattt cgccatgacg acatcgaaac
     2221 gatcctgctc gatcccgaaa cctacagcag tgcctcgcgc cggctggcgg taccgaacgg
     2281 gatggatgcg cccgaacaca ccgcatatcg cgcggcccag gagcccttct tttcgagcga
     2341 ccagatggcc actttcgatt cccagtgtcg caaggtggca cgggagcttg tcggcccact
     2401 cgtggcccgg tcgaccgtcg agatcgtggc tgatttcgcg gacccgtttt cgatgcgatc
     2461 gctctgtgca ttcctcggtt ggccggagga gaactggggc tacctgctcg gatggacgca
     2521 cgggaatctg caagcagcct acgcgcgcga tcgggacgcg ggtaagacgc tggccgaaga
     2581 attcacacac tacgtcatgg agcagatcga tgcgcgccgg acggacggcc ggtctgaatc
     2641 cggtgacgac gtcacaagcc gactgatgcg caggacggtg aacgggcgcc ggatgacgga
     2701 tgaggaactg atcagtgcgc ttcggaactg gactgccgga catggcacag ttgttgccgg
     2761 cctcagcaat ttgctcttgt acctggctcg tcatccgcaa ttccaggatc atctgcggtc
     2821 tgaacccggc ctgatccctg ccgctgtcga cgaggtgctg cgagtcgatg ggccgctcgt
     2881 gtcgaatcgc cgcacgacga cgcgtgaggt cgaaatcggc ggtcgcgtga ttgacgccgg
     2941 gcagaacctg accttgatgt ggcttgccgc cgaccgggat ccgcgcgagt ttgatgaacc
     3001 gaccaccgtc aacctcgatc gcgatcaggc gggtaacttc ctgtttgggg caggtatcca
     3061 ctactgcgtc ggcgcaccca tggctcggct cgagctccgg atcgccctcg aagagctgct
     3121 ctcccacacc cgcgagcttg ccttggctgg aaatccgaat ccgcccaggt caacctatcc
     3181 aggcaacggg gtggaggtgc tccagatgca cctgcgctga tccaggtcga gccaggttgg
     3241 ctgccaacct ggtagcgaac gctgctcagc ccgctcatca atatcgtggc aatgcatccg
     3301 attgcgacat gatgtcgtct aactaacgcc agcgctttgg cagccgatag ttcccgttgg
     3361 gagggatcga gatgcatcgt gcagtcgcgt tcgtagcgat tctttgttcg tgcctgatga
     3421 gcggagtggg catcgctgtt gctcagcagg ccacgcctga caatgcggcg ttcagcggaa
     3481 tgccggagct cacggtgacc atcaccgacg acgcagtcca ggtcgataag acggagatcc
     3541 cggacggcta cgtgctcctg aacgtggtta ataactcaaa ggacaacaac agcgccggcg
     3601 tgcttggccc cggccccgga cgcacgatgg acgacttgaa agccgccgcg gccaccccgg
     3661 aagccagtga cgaaatcccc ccgttcctct atgaagcgac catcctcggc ggtcccggcg
     3721 atatcgaccc gggcaagtcg gcccaggccg tgatcaacgt gccggctggc gattgggcgg
     3781 tcttcgccga aggcagccag ccacctgtgt tcatcagcgc gaagcaaacg gccgattcga
     3841 gcaccaggcc gcccacggcg gataccaaga tcgagctcgg cgacttctac ttcaacggct
     3901 tttcggaggg agtgaaaccc ggcccgcagg tctgggaagt cacgaacacc ggcaagcaga
     3961 tccacatgat ggtcgtcggc aaggtgccag atggcaccac cgagcagcag gtgatcgaca
     4021 ccttcaccgc gcaggaaacc ggcacgccag tggccggcgc tctacaggag tcggacctcc
     4081 agccagccgg caccggcgtg ctgctcatat cgtccggaca aacgatgtac gtaaccgccg
     4141 atctcgaggc gggcacgtac gttgcgctct gcttcgtgac cgatccgcaa acccacaagc
     4201 ttcacgctat ggaaggcatg gtttcggtct tccaggtcgg ccaaggcacg gccacgccga
     4261 cctcctgaca agcgacacgt gaacgaaacc gggacgaatc gttcgtcccg gtttctcctt
     4321 ggccgcgtac tattcccgga tcgcggccgt tttgccaacc tgcaatccat gaggtcacgt
     4381 gtcggtcctg ctcgtccgaa tcgataagca tcaaatcgac gtcgaagtgg cgcggaagga
     4441 gatccgccac ctctacatcc gcgtcgatcg gattgatggt cgggtacggg tttccgcgcc
     4501 gcgcgggatg agtgatgacg caatccggga cgcagtccgc cggaatcatg actggatcgc
     4561 tcgacgccgg gaaagcgcca ccagccggga atcgccggcg cggttggaac tcgtcacggg
     4621 cgatctcgtt cctgtcctcg gggaccaggt ccatttgtac gtccgcgaat ccaatacggg
     4681 gcgccggagt gtccatctcg acgcggaggg ccggttggcc atgcacatag cgccgggatc
     4741 tacccgagag acccgcgcca cgctgctcga tgactggtac cgccgacaac tgcgcgatcg
     4801 cataccggtg ctgatcaagg cgtgggagcc ggagatgggt gtgtcggtcg cggagtgggg
     4861 cgtcaagcgg atgcggagcc gctggggcag ttgcaaccca cgggcgcgga ggatctggct
     4921 gagtcttgag ctcgcgcggc gaccagtcgc gtgcctcgag tatgtcgtcg ttcacgagat
     4981 ggtccatctg ctcgagcgag gtcacaaccg gcgcttctat gcattcatgg accagttttt
     5041 gccggactgg cgcctggcgc gagcggagtt gcgaagttcc ccgcctgact aggatggtat
     5101 tctgcgctcc gcggccagga tcggttggat ccatccctgc tccaaacctt ccaattccac
     5161 cgaggtgttc aagcgcgggc atgacctttt cgggaatcga gcgttttgat cgatcgggcg
     5221 ggaacgcgga agggaaattg cgacgtgccg aatactgcga gcgggaatga tcaggcagga
     5281 ctggctctac cctgtgggcg gagtctgaac cggcgatctc tccttcaggg agcagtcggc
     5341 tcggctggtc tcacgctgtc accggtgcaa ttggccgcag cccagggcgc ggctactccg
     5401 gttccgcaga cggaccggcg ggtgcgggtg ttcccggtgc ccgattccat gaccgcttcg
     5461 cccggaacac agcttagttt ccgggggccc ggcctcgcga ccgtccagcc gatcgaagtc
     5521 atcggctccg agagcggcgt tcatcccggt gttgaagtgg cgcactccga cggaaacggc
     5581 atgagctgga tacccgactt cggtttccgc cccggcgaag cggtgaccgt ttcgacgcgc
     5641 cttgacatcg cggggacaag gaaaggcgac ttctcgttca ttgtttcgac accccggccg
     5701 ctggcggcac atccgcggcg caacaagaag ccggaggaga agcaggttca tcacttccga
     5761 agccggcccg gtctcgcgcc gcaagtgttc gatgttacgc aacatgggga atcgctcgcg
     5821 cccggctatc tcttcgacgc cccgaaggag ggcgcgggcc ggaacggcgc gctgatcatg
     5881 gataacagtg gcgagccggt ctggttcttc ccggtggagg cgccggtcga ccagatcctg
     5941 gatttccgcg tccaggaatt ggacggcgag ccggtcctga cctggtggca gggcgtcgtc
     6001 gtgcgcgggc acggattcgg ccactgggtg atccgggacg gctcgtatga ggagatcgcc
     6061 aatatccgaa tcggcaacgg ctatgccggc ggcgacctgc atgaactggt cctgaccgaa
     6121 gagcggacgg cgctggtcgg gacctacagc acgattgcgt gggacctcac ctcaataggc
     6181 ggcaagaagg acgacagcat cattgactgc atcgtccagg agatcgatat cgccaccggc
     6241 gcggtcatgt tcgagtggca tagcctcgat cacgtcgcgc ttgacgagac gtatgacaag
     6301 accgacgagg gcgatgagga ggatgcgctt gatcacttcc acttcaacgc gatcaccctc
     6361 gacagcgatg gcaacctggt catcagcgcg cgcaacacct gggccgccta cacgatcgat
     6421 cgggagacgg gagacgtgat ctggcgtctc aacggcaagg ccagtgactt cgtggtcggc
     6481 gacggcgccg acatggccta ccagcacgat gttcgccact ggcc
//
LOCUS       DASSGX010000509         2516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1069460, whole genome shotgun sequence.
ACCESSION   DASSGX010000509 DASSGX010000000
VERSION     DASSGX010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069460"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="VFI12_09165"
     CDS             complement(<1..385)
                     /locus_tag="VFI12_09165"
                     /inference="COORDINATES: protein motif:HMM:NF015791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HET7056621.1"
                     /translation="MTTTGVDGLELLTAAEMGRADELTIAGGTPGLTLMENAGRGIAE
                     EVEWVAEPGARIAVLCGPGNNGGDGFVAARLLRERGYSVSVALLGSREALRGDAAEAA
                     AAWNGPIWPLSTAFAEDAAIVIDALF"
     gene            complement(406..786)
                     /locus_tag="VFI12_09170"
     CDS             complement(406..786)
                     /locus_tag="VFI12_09170"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7056622.1"
                     /translation="MRVTGFDHLVLTVASIERTVEFYERVLGMTREVFGPERRTALRF
                     GPHKINLHEHGKEFEPKAAKPAPGSADLCFLVEGFDNAADHLAAAGVPILEGPVERTG
                     ARGKIVSYYIRDPDMNLIELSHYS"
     gene            1102..1440
                     /locus_tag="VFI12_09175"
     CDS             1102..1440
                     /locus_tag="VFI12_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HET7056623.1"
                     /translation="MKKIEAVIKPFKLDEVKEALQEIGLQGITVLEAKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIELVIADELLDRAVEAIQRAAKTGRIGDGKIFISSIEEAIRI
                     RTGETGTEAI"
     gene            1536..>2516
                     /gene="glnA"
                     /locus_tag="VFI12_09180"
     CDS             1536..>2516
                     /gene="glnA"
                     /locus_tag="VFI12_09180"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HET7056624.1"
                     /translation="MANPADVLKLIKDKDVKFVDLRFTDTKGKMQHVTAEASCVDESV
                     FADGYAFDGSSIAGWKGIETSDMLLSPDPATAHLDPFFAQTTLAIFCDVIEPMTGELY
                     ERDPRGVAKKAEAYLKQTGVGDSVFFGPEAEFFIFDDVRFTADPFNTGFRLDSTELPT
                     NSGTDYEMGNLGHRPRTKGGYFPVPPVDSCQDIRSEMLSVMAEMGVSVEKHHHEVAAA
                     QHELGIKFAPMVFMADHLQIYKYVAHNVAQAYGKSATFMPKPVFADNGSGMHCHQSIW
                     KDGQPTFAGNKYSDLSDTCLYYIGGILKHAKAINAFSNPTTNSYKRLVPGY"
BASE COUNT          497 a          853 c          768 g          398 t
ORIGIN      
        1 cgaacagcgc gtcgatgaca atggcggcat cctcggcgaa agccgtgctc aaaggccaga
       61 tcggcccgtt ccacgcggcg gccgcctcgg ccgcgtcgcc cctgagggcc tcgcgcgacc
      121 cgagcagcgc cacgctgacc gagtaaccgc gctccctgag caggcgcgcg gcgacgaagc
      181 cgtcgccgcc gttgtttccc ggtccgcaga gcacggcaat gcgcgcgccg ggctcggcga
      241 cccactcgac ctcctcggcg atgccgcggc cggcgttctc catgagcgtg agacccggcg
      301 ttccgcccgc gatcgtcagc tcgtccgcgc ggcccatctc ggccgccgtc agcagctcca
      361 gcccgtcaac gccggtcgtg gtcatcgctg gccccgctaa ggatcttagg agtaatggct
      421 gagctcgatc aggttcatgt ccggatcgcg gatgtagtac gacacgatct tcccccgggc
      481 accggtgcgc tcaacgggtc cctccaggat cgggacgccc gccgccgcga ggtgatcggc
      541 agcattgtcg aaaccctcga cgaggaagca cagatccgcc gagccgggcg ccggtttcgc
      601 tgccttgggc tcgaactcct tcccgtgctc atgcaggttg atcttgtggg gcccgaaccg
      661 cagcgccgtg cgccgctccg gaccgaacac ctcgcgcgtc atgccgagaa cgcgctcgta
      721 gaactcgacc gtccgctcga tcgaggcgac cgtgaggacg agatgatcga aacctgtgac
      781 gcgcaatgga agcctccctg tcctggacaa gtcgcatcgt ctgcggcctc cgaagtcaac
      841 cccgccagcg gcatcacgta cagcgcgaag tgcgttccaa ggctgcctat ttcttcggcg
      901 atatgcggca acctcgatgc ttaaaaattc atcacaactg ctcgacaatt cagcagggcg
      961 gtccgtcgag gacgggcagg actgaaccgg caggccagca aagcattgat gtgccgaagg
     1021 aacgtgccgc agcatccaac tggcacgcgt catgcttaat tcgaggtgag ccaacaagca
     1081 gtcccgtccg ggaggcaggg aatgaaaaag atcgaggctg tcatcaaacc cttcaagctc
     1141 gacgaggtga aggaggcgct tcaggagatc ggcctgcagg gcatcaccgt gctcgaggcc
     1201 aagggcttcg gccgccagaa aggccacacg gagctctacc ggggcgccga gtacgtcgtc
     1261 gacttcctgc cgaaggtcaa gatcgagctc gtcatcgccg acgagcttct cgaccgggcg
     1321 gtggaggcca tccagagggc ggccaagacc gggcgcatcg gcgacggcaa gatcttcatc
     1381 tcctcgatcg aggaggccat ccgcatccgc accggcgaga cgggtacgga ggcgatctga
     1441 ccacagtagg gctcacccga cccgcgcatc cgcgcggcct tccgagctca atgcaaacac
     1501 ccacacccaa gcctgcacac gaggggagtt gttcaatggc aaacccagcc gacgtcctga
     1561 agctcatcaa ggacaaggac gtgaagttcg tagacctgcg gttcaccgat accaagggca
     1621 agatgcagca cgtgaccgcg gaggcaagct gcgtcgacga gagcgtgttc gccgatggct
     1681 acgcgttcga cggctcgtcg atcgcaggct ggaagggcat agagacctcc gacatgctct
     1741 tgagccccga tccggcaacg gctcacctgg acccgttctt cgcccagacc acgctcgcca
     1801 ttttctgtga cgtcatcgag ccgatgaccg gcgagctgta tgagcgcgac ccgcgcggcg
     1861 tcgccaagaa ggccgaggcc tacctgaagc agaccggcgt cggcgacagc gttttcttcg
     1921 ggcccgaggc cgagttcttc attttcgacg acgtgcgctt caccgccgat ccattcaaca
     1981 cgggtttcag gctcgactcg accgagctgc ccaccaactc cggcaccgac tacgagatgg
     2041 gcaacctcgg ccaccggcca cgcacgaagg ggggctactt ccccgtgccg ccggtcgata
     2101 gctgccagga catccgctcc gagatgctgt ccgtgatggc agagatgggc gtctcggtgg
     2161 agaagcatca ccacgaggtg gcagccgccc agcacgagct cggcatcaag ttcgcgccga
     2221 tggtgttcat ggccgaccat ctgcagatct acaagtacgt ggcccacaac gtcgcccagg
     2281 cctacggcaa gtcggccacg ttcatgccga agccggtgtt cgccgacaac ggctcgggga
     2341 tgcactgcca ccagtcgata tggaaggacg gccagccgac gttcgccggc aacaagtact
     2401 ccgacctttc cgacacctgc ctctactaca tcggcggcat cctgaagcac gcgaaggcga
     2461 tcaacgcctt ctcgaacccg acgacgaact cttacaagcg tctggtcccc ggctac
//
LOCUS       DASSGX010000510         3033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1180725, whole genome shotgun sequence.
ACCESSION   DASSGX010000510 DASSGX010000000
VERSION     DASSGX010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180725"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..1895)
                     /locus_tag="VFI12_09185"
     CDS             complement(147..1895)
                     /locus_tag="VFI12_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056625.1"
                     /translation="MTDTREVPIESLVVADFGRGTSRAYLLEAISGAFRFVAKAEHRT
                     TTDLPYEDLSQGWYALLRQLEWASGRGLTVRDRLAVPQLASGDGVDGLLISASLGEPI
                     RIAILEAGQSAVAGPVLDALRRVHTRVFHAAAPSSRKDGGWAATQADALRSFQPEMAL
                     LIVGAGAQDAMPRLLQLAKQIGVIGTVSRAIVIADGQAQEQGVAALGSKLKVRSISPV
                     VRAPADIAQEIERELGEAFQIRLRTSDFDDIAEDAMQAPISRAHAVDLVNRFIARAFK
                     RQVLTIGIDDGTHVHWAAGDQGAISALPQVDLATSMSGLTSRDVADATCWLPFEISED
                     ELITWALNRSIRPWTIAEQPRDLAIEQAMARQVARRAIAEISRAQPMALSGVDLVIGG
                     PAFARWNQPGAAALALLDSLDVVPNNGVVDLALDPDGLMSVAGIVGTIDPNLASSLFE
                     YDALTHLGSAIVIGGATSPGDVACRGELHFDSGEMAQFSVLSGTVEVIPLSTGETATI
                     VLRPERKFSVGGHPAGKTVTLAEERRIIGGTVGVIIDARPRSLATNGNRAAQVRQWFE
                     AVNGIRGTTAVRKTAG"
     gene            complement(1935..2720)
                     /locus_tag="VFI12_09190"
     CDS             complement(1935..2720)
                     /locus_tag="VFI12_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056626.1"
                     /translation="MNLCDNCGTANGELETECRICGHALAGMAAPAETAAPPAASESP
                     PTSYAAADSAPELQISSVRQSMRTGAPPMLGNAGTPDPVTPDAAQKSALPSFMQEGAR
                     AQAPAPEPVNLISANDLPEWIRQIAEADAAKAEAEAAAAARQTADAPAPIAKRSLPGT
                     ESSTAPSTTWLSKSSAAAETVDHWAAESPAATEPAQPAYPTITPSHTYMPSLHELSAA
                     SAKKRKLGRNTSAGASGAPVYRNRLVQLAALVLLLALLAAMVL"
     gene            complement(2891..>3033)
                     /locus_tag="VFI12_09195"
     CDS             complement(2891..>3033)
                     /locus_tag="VFI12_09195"
                     /inference="COORDINATES: protein motif:HMM:NF014621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0051499 - D-aminoacyl-tRNA deacylase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HET7056627.1"
                     /translation="VDQFARSVSSQGIATATGTFGAHMVVRLANDGPVTIWIDSADLT
                     RD"
BASE COUNT          496 a          969 c         1035 g          533 t
ORIGIN      
        1 cgccgctgat ccgctcacct gcccggacct tgatttcggc attacccggg agtcgccgca
       61 tgacgtgaag ctcctggtca tggacgatca cggcttcacg attcgggaaa ttctggttca
      121 tgatcctgat cctcagttcg gagcgactag cctgcggtct tgcggacggc cgtcgtgccg
      181 cgaatgccgt tgacggcttc gaaccactgg cgaacctgcg cggcgcggtt cccgttggtg
      241 gcgagtgaac gcggtcgcgc gtcgatgatc acgccgaccg tcccgccgat gatccgtcgc
      301 tcctcggcca gggtcacagt ctttccagcc gggtgtccgc cgacggagaa cttgcgctcg
      361 gggcggagga cgatcgtcgc cgtctccccg gtactgagcg ggatgacttc gacggtgccg
      421 gacaggacgc taaactgagc catttcgcca ctgtcgaagt ggagttcgcc gcgacaggcg
      481 acgtcgcccg ggctggttgc gccgccgatc acgatcgcgc tgccgagatg ggtgagcgcg
      541 tcgtactcga agagactcga cgccaggttc ggatcgatgg tgccgacgat gccggcgacg
      601 gacatcaggc cgtctgggtc gagtgcgagg tcgacgacac cgttgttcgg gacgacatcc
      661 agggaatcga gcagggccag tgccgccgct ccgggttgat tccagcgggc gaaggcagga
      721 ccgccgatca cgaggtcgac tccggagagg gccatcggct gtgcgcgtga gatctccgcg
      781 atggcccggc gggcaacctg ccgcgccatc gcctgttcga tggcaaggtc gcgcggctgc
      841 tcggcaatgg tccacggccg gatagaccgg ttgagcgccc aggtgatcag ctcgtcctcg
      901 ctgatctcga agggcagcca gcaggttgca tcagcaacgt cacggctggt cagcccggac
      961 atcgacgtcg caaggtcaac ctggggcagg gcagagattg caccctggtc gccggcggcc
     1021 caatgcacat gcgtgccatc gtcaatgccg atggtgagaa cctgccgctt gaatgcgcgg
     1081 gcgatgaagc gattgacgag gtcgaccgca tgcgctcggg aaatcggcgc ctgcatcgcg
     1141 tcttcggcga tatcgtcgaa atcggatgtc cgcaatcgaa tctggaaggc ctcgccgagc
     1201 tcacgctcaa tttcctgcgc aatgtccgcc ggagcgcgca ccaccggcga gatggaccgc
     1261 accttgagct tcgagccaag ggccgcaacg ccctgctcct gggcctgacc gtcggcaatg
     1321 acgatggcgc gggaaaccgt gccgatgacc ccgatctgct tggcgagctg tagcaggcgc
     1381 ggcatggcgt cttgtgcacc ggcgccgacg atcagcagcg ccatttccgg ctgaaacgag
     1441 cggagggcat cggcctgcgt cgctgcccaa ccaccatcct tgcgggatga gggcgctgcg
     1501 gcgtggaaca cgcgcgtgtg aacgcggcgc agggcgtcga gaaccggacc agcgactgcg
     1561 ctctggcctg cctcgaggat cgctatgcga atcggctcgc cgagggatgc cgagatcagc
     1621 aatccgtcga ccccgtcgcc gctcgccaac tgcggcaccg ccagtcggtc ccggacagtc
     1681 aggccgcgtc cgctggccca ttccagttga cgcaacaggg cataccagcc ctgcgaaagg
     1741 tcctcgtacg ggagatcagt cgtggtcctg tgctctgcct tggctacgaa ccggaatgcg
     1801 ccgctaatcg cctccagcag ataggcgcgc gacgtgccgc gaccgaaatc ggccacaacc
     1861 agcgattcga ttggtacttc acgagtgtcg gtcatgcgtg ggactccgta acccttggcc
     1921 gcccgggcgt gccgttagag gaccatagcc gcgagcaatg ccagcagcag gacgagcgcg
     1981 gcgagctgca ccaaccggtt tcggtagacc ggcgcaccgc tggcgccggc cgacgtgttg
     2041 cggccgagct tccgcttctt cgcggaggct gccgagagct catgtagcga cggcatgtag
     2101 gtgtgcgatg gagtgatcgt cgggtaggcg ggttgggccg gctctgtcgc ggctggcgac
     2161 tcagccgccc agtggtcgac cgtttccgct gcggccgagg acttgctcag ccaggtggtt
     2221 gacggcgcgg tcgacgactc ggttcccggc aacgaccgct ttgcgatagg tgccggtgca
     2281 tcggcggttt gccgggctgc cgctgccgcc tcggcttcag cctttgcggc gtcggcctcg
     2341 gcaatctggc ggatccattc cgggagatcg tttgccgaga tcaggttcac cggttcgggc
     2401 gcgggcgcct gggccctggc gccctcttgc atgaatgatg gaagcgccga tttctgcgcg
     2461 gcatccggag tgaccggatc cggcgtcccg gcattaccga gcatcggcgg cgcgccggtg
     2521 cgcatggact ggcgcacgga agagatttgc aactctggag ccgaatcagc tgctgcgtag
     2581 gacgtgggcg gcgattcgct tgccgctggt ggtgccgctg tctcggcagg cgccgccatt
     2641 ccagcaagcg cgtgcccgca aatccggcac tcggtctcga gctcgccgtt cgcggtgccg
     2701 cagttgtcgc aaagattcac gggtcgtccc tccatcggcc tcgctcgccg ggctggcaac
     2761 acatcggcgg cgcctgctca tcggttgccg agcatgtcgc actggaaatc gagtataggg
     2821 agggggtccg ggctgctccg cagctggaga caccgaaaag tcccgatgtt gacgtggtca
     2881 accggtgggt ctaatcgcgc gtgaggtccg cgctgtcgat ccagatcgtg accggaccat
     2941 cgttggcgag gcgcacaacc atgtgcgcgc caaacgttcc ggtcgcggtc gcgattccct
     3001 gcgagctgac gctgcgcgca aattgatcga cca
//
LOCUS       DASSGX010000511         3629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_418579, whole genome shotgun sequence.
ACCESSION   DASSGX010000511 DASSGX010000000
VERSION     DASSGX010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418579"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="VFI12_09200"
     CDS             <1..570
                     /locus_tag="VFI12_09200"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HET7056628.1"
                     /translation="FVAGIDEAGRGALAGPIIAAAVVLPPGLRLTARVREINDSKQLA
                     EAKRDRLADLVRSVATAWSIGQATASEIDELGISVANRLCMERAVARLGCEPDFALLD
                     AFTCDLSVPQIGLIDGDAISLSVAAASILAKTERDHFMRKAHEQDCRYHFDRHVGYGT
                     AIHLDALRSHGPCQLHRLTFRGVRPEAAE"
     gene            567..941
                     /locus_tag="VFI12_09205"
     CDS             567..941
                     /locus_tag="VFI12_09205"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HET7056629.1"
                     /translation="MTTSRTKLGSVGEHHARLMLERKGFRFVEANWRAASGEVDLVML
                     DGEFLVFVEVKVRRGERAGTAEESISRAKAQRLLATGDWYVAEHPEYAERPWRIDLMA
                     ITIDDRGVVARSLHIEDAVVAG"
     gene            1015..2352
                     /gene="hisS"
                     /locus_tag="VFI12_09210"
     CDS             1015..2352
                     /gene="hisS"
                     /locus_tag="VFI12_09210"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HET7056630.1"
                     /translation="MTETQTTGRRVKAQVLSGFRDQTPAQMLLRRALIDRFRAIFEAH
                     GFEPIDTPTLEHLEVLTGKAGENEKLMYHFVDHGDRAVGMRYDLTVPLARFVANHQNE
                     LPMPFKRYHIAPVWRAERAQRGRFREFWQCDADIVGSSSARADAEAISMVAEILEAAG
                     LPQFTVSISHRRLLEGLARNAGVPGYLAPTVYRAVDKLNRIGPGGVRTELIEAGVSDE
                     AAERLLALISQSGDNRALIESLRERIGDDPEVLGALENLAELFTLLESLAPGKRCFRL
                     DLTLARGIDYYTGIVYEATVDEPKVGSVSGGGRYDGLVGMYSGRDIPATGISIGLERI
                     IEVAEEFNLVSAPKSVCDAIVIYHDAVFDYASAIARTLRNDGFNIDLSLLGRKSFGDQ
                     LKYADRRGIPLAILIGEDEAAAGTATIKDLAAGNQETVAATLLPSVLASISDR"
     gene            2349..3206
                     /locus_tag="VFI12_09215"
     CDS             2349..3206
                     /locus_tag="VFI12_09215"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HET7056631.1"
                     /translation="MIRFGPAGAPRWFNQNLALLEHYLDLIAEEGGSAIEFVVLPDPG
                     TEELGRVHLLGPIAPAAIARAQARGLMVNLHAPLTPAFRVAEWARDRDSYRNRFLPII
                     DLLRVTEVGQAEPPVFVIHAAAGRPDQTSNYLEWLAETLSGAHLAARIAVELRGPGSG
                     DFHEFDRSLATLSAFVDGIGDDRIGICWDVAHDWEHGGDIAELGPDLARHINHVHVHD
                     NRPTGEVHAPLGSGSVPWRDALRQLNDAGWSGSVTLEIRYRYACELGDPWDILRASLQ
                     AARAVLAGE"
     gene            3216..>3629
                     /locus_tag="VFI12_09220"
     CDS             3216..>3629
                     /locus_tag="VFI12_09220"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HET7056632.1"
                     /translation="MPTGADWDKYDRYLVVVAHPDDAEFSSAGTISCLTAAGKHVVIA
                     QVTSGDKGTSDPQTATTHMVTTREAEEVEAARRLGVETVDFLRQTDGELMPTLELRGL
                     IAREIRMHTPDVIITHDPFRPYAFHPDHRAVGLSTH"
BASE COUNT          642 a         1130 c         1193 g          664 t
ORIGIN      
        1 tttgtcgccg gcatcgacga ggccgggcga ggcgccctgg ccggcccgat catcgccgcg
       61 gcagtcgtcc tgccgccggg cttgcgactg accgccaggg ttcgcgagat caacgattcc
      121 aagcaactcg cggaagcgaa acgcgatcga ctggctgacc tggtgcgctc ggtcgccact
      181 gcctggtcga tcgggcaggc gaccgcgtca gaaatcgacg aactcggtat ttcggtcgcc
      241 aaccggctct gcatggagcg ggccgtcgca agactcggct gcgagcccga cttcgccttg
      301 ctcgacgcct tcacatgtga cctgtcagtc cctcagatcg gactgatcga tggcgacgcg
      361 atttcgttgt cggttgcggc cgcgtcgatc ctggcgaaaa ccgagcggga ccacttcatg
      421 cgcaaggcgc acgaacagga ttgccgctac cacttcgacc gccacgtcgg ctacggcact
      481 gcgatacatc tcgacgcgct gcgatcacac ggcccgtgcc agttgcatcg cctgaccttt
      541 cgcggcgtcc gcccggaggc cgccgaatga cgaccagccg tacgaagctt ggttccgtcg
      601 gtgagcatca cgcgcggctg atgctggagc ggaagggctt ccgcttcgtc gaagcaaatt
      661 ggcgcgcggc atcaggcgag gtcgatctgg tcatgctcga tggcgaattt ctcgtcttcg
      721 tcgaggtcaa ggtccgccga ggtgaacgcg ccggaactgc cgaggagtcg atttcccgag
      781 cgaaagcgca gcgattgctt gccactggcg actggtatgt cgccgagcat cccgaatatg
      841 ccgagcgacc gtggaggatc gacttgatgg cgattacgat tgacgaccgg ggcgtagtcg
      901 cgcggagcct gcacatcgag gatgccgtcg tcgcgggttg accggccagt cccgtagaat
      961 cagagcggtt tctcgcgcac tggcgcgtcc ctcctgacag gagttagcag ttcgatgaca
     1021 gagacgcaga cgaccgggcg ccgcgtcaag gcgcaggttt tgtccggatt tcgcgatcag
     1081 acgccggcgc agatgttgct gcgtcgggca ctgatcgatc gcttccgggc cattttcgag
     1141 gcgcacggct ttgagccgat cgatacgccc acgctggagc acctcgaggt cttgaccgga
     1201 aaggccggcg agaacgagaa gttgatgtat cacttcgtcg atcatggcga ccgcgcagtc
     1261 gggatgcgct acgacctgac ggtgccgctg gcccgcttcg ttgcgaacca tcagaacgaa
     1321 ttgccgatgc cgttcaagcg ctatcacatc gcgccggtct ggcgggcgga acgggcacaa
     1381 cgtgggcgat tccgcgagtt ttggcaatgc gacgcggata ttgtcggttc atcctctgca
     1441 cgcgccgacg ccgaggcgat ctcgatggtc gcggagattc tggaagcagc cgggttgccg
     1501 caatttacgg tcagcatcag ccaccgccgc ctgttggaag gcctggcccg aaatgccggt
     1561 gtgcccggat acctcgctcc aaccgtctac cgcgctgtcg acaagctgaa ccggatcggt
     1621 ccgggcggcg ttcggaccga attaatcgaa gccggcgtat ccgacgaggc ggccgagcga
     1681 ttgctcgcgc tgatcagcca atccggggac aatcgggcgc tgatcgaatc gcttcgtgaa
     1741 cgcatcggcg acgatcccga ggtgctgggc gcgctcgaga atttggccga gctgttcacg
     1801 ttgctcgaat cgctcgcgcc cggcaagcgc tgcttccggc tcgacctgac gcttgcccgg
     1861 gggatcgatt actacacggg gatcgtgtac gaagcgacgg tcgatgagcc gaaggttggc
     1921 tctgtctcgg gcggcggccg gtacgacggg ctggtcggta tgtattccgg ccgcgatatc
     1981 ccggcaacgg gcatctcgat cgggttggag cggatcatcg aggtggccga ggagttcaac
     2041 ctcgtctcgg cgccgaaatc ggtctgcgac gcgatcgtga tctaccacga cgccgtcttc
     2101 gactatgcca gcgcgatcgc caggacgctg cgaaacgacg ggttcaacat agacctctcg
     2161 ctccttgggc ggaagagctt cggcgatcag ttgaagtacg ccgaccgccg tggcatacct
     2221 ttggcaatcc tgatcggcga ggacgaggca gctgccggaa ccgcgacgat caaggacctg
     2281 gcagccggca accaggagac cgtcgccgcg acgttgctgc cgtcggtcct tgcgtcgatc
     2341 agcgaccgtt gatccgattc ggtccggccg gggctccgcg ctggttcaac cagaatctcg
     2401 ccctgctcga gcattacctg gacttgatcg cggaggaggg cgggagcgcg atcgagttcg
     2461 ttgtcctgcc ggatcccggc accgaggagc ttgggcgggt tcacctgctc gggccgattg
     2521 ctccggcagc catcgctcgt gcccaggcga gagggctgat ggtcaacctg catgcgccgc
     2581 tcacgccggc gtttcgggtc gcagaatggg cacgggatcg agactcatac cggaatcgct
     2641 tcttaccgat tatcgatctc ctgcgggtga ccgaagtcgg tcaggccgag cctcccgtat
     2701 tcgtgatcca cgcggcggcg ggacggccgg accagacaag caactacctt gaatggctgg
     2761 ccgaaaccct ctcaggggcg catttagcgg ctcggatcgc ggtggaactg cgcggtcccg
     2821 ggagcgggga ttttcacgaa ttcgaccgct cgctggcaac actctccgca ttcgtcgacg
     2881 ggattggcga cgacagaatc ggcatctgct gggacgttgc ccacgactgg gaacatggcg
     2941 gcgacatcgc tgaattggga cctgatctcg cgcgccatat caatcacgtc cacgtccacg
     3001 acaaccgacc gaccggcgaa gtccatgcgc cgctgggttc gggctcagtt ccgtggcgcg
     3061 atgcgctgcg gcaactgaat gatgcggggt ggagtggctc cgttaccctc gagattcggt
     3121 atcgttatgc gtgtgaactg ggcgacccgt gggacatcct ccgggcgagt ttgcaggctg
     3181 cgcgtgcagt cctggctggg gagtaagaca agcagatgcc aaccggcgcc gactgggaca
     3241 agtacgatcg atacctcgtt gtcgtggcgc atccggacga tgctgagttc tcgtcggccg
     3301 ggaccatctc ctgcctgacc gccgccggca agcatgtcgt gatcgcgcag gtaacctcgg
     3361 gcgataaggg tacctccgat ccccagaccg cgacgacgca catggtcacg acccgcgagg
     3421 ccgaggaggt cgaagcggcg cgacgcctcg gcgtcgagac ggtcgacttc ctccgtcaaa
     3481 ccgacgggga gctgatgccg acgctcgaac tccggggcct gatcgctcgg gagatcagga
     3541 tgcacacgcc cgatgtgatc atcacgcacg acccgttccg gccgtatgcc tttcatccgg
     3601 accatcgggc ggtcggtctt tccacgcac
//
LOCUS       DASSGX010000512         3637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1180753, whole genome shotgun sequence.
ACCESSION   DASSGX010000512 DASSGX010000000
VERSION     DASSGX010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180753"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="VFI12_09225"
     CDS             complement(<1..727)
                     /locus_tag="VFI12_09225"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7056633.1"
                     /translation="MSADIVLINGHVVTVDPMNPVASAVAIRAGHVVHVGSEADARAA
                     AGPRAEVIDLRGRTATPGLNDAHAHPMMVGQALLDVDITPERVGSVDGIARAVKGRIN
                     GQEPGTWIIGRGYDQARMTDQRHPTRADLDAVSPDHPVFLYRACHHIAAVNSFALQLA
                     GITADTRDPEGGSIDRDENGEPTGVLRESAMELVHAHIPEPTEDSIMRALELAGRAFL
                     RTGVTSTVEAGIRQPKQMSAYQRL"
     gene            complement(750..1565)
                     /locus_tag="VFI12_09230"
     CDS             complement(750..1565)
                     /locus_tag="VFI12_09230"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET7056634.1"
                     /translation="MARDGFTRDRWPGGHRCALCLSFDVDGPYGEANYQPPTNTYAIS
                     QTEYDPFGTQRILAILSDADVPATFCWVGKEAQDRPELVRAAFEQGHEIALHSWDHRY
                     YHEMSPDEQRTDMERTSDTLTEITGERPTGHKTAGWRYTADTHRICQALGLRWVMDIP
                     RGDLPYLLQPDRALDPLVQLPPSAHWDDYSFFVDKMLTPGQAFEFWRDDLDVIRSEGS
                     MMCLTMHPFVSGRPGPSRAIIRLIDYAIDLGDVWIARADQIADWWLTQHQAGV"
     gene            complement(1591..2811)
                     /locus_tag="VFI12_09235"
     CDS             complement(1591..2811)
                     /locus_tag="VFI12_09235"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET7056635.1"
                     /translation="MVTKQTATAPVESNGSFPDREKLDELRNQLPAVTATRYFNAGSN
                     GPVPIPAHQALVDQAIEDLNAGRCDLAFYGKRKDESTQLRETIAGIFNANVSEVAIMR
                     STTEGMNAALNGVLWRPGDEIITTQLEHICLYSAIGSVSHRFGVTVRTVDIADGGGDV
                     LGALAKAVTPRTRVIAISHVQWTTGAIMPLKEIADFARERGILTIIDGAQGAGHVAVD
                     FHALGVDAYCQAGQKWLCGPNSSGFMLLRDDRLGDFRTSYLRYGTFDTAGYVTPPPGA
                     ARFEMGETYGPAIAAFNTGLEWLRDKAGFDWLVARNAALGKRCLDALKKIDGVTVLTP
                     GGKMAGMVNFNIAGMHPKDVSDRLAERGQTIRFVDQRPAQASARISTAWWTTEEEVDD
                     LVAAIANLASSKAE"
     gene            complement(2942..>3637)
                     /locus_tag="VFI12_09240"
     CDS             complement(2942..>3637)
                     /locus_tag="VFI12_09240"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET7056636.1"
                     /translation="IGTFCTHTLVHAKQCVKVSKELPPEQMSLIGCGVMTGVGAALYT
                     ADIQPGQSIAVVGCGGVGDSVIMGAKLAGATTIIAVDIDPQKLEWARGFGATHTVNPK
                     DGDPVAAIKEITGGFGVNASFEAVGKPETLMTALWARDLAGTCVIIGVAGQDAMVELP
                     YAKFFDLGGALKVSWYGDCLPTRDFPLLAAWYSQGRLDLDGVVTRVISLEESEEAFHA
                     MERGETLRSVIKL"
BASE COUNT          633 a         1133 c         1188 g          683 t
ORIGIN      
        1 ggagtcgctg gtaggcggac atttgctttg gctgtcgaat cccggcttcg acggtgctgg
       61 tgacgccggt gcgcaggaac gctcgacccg caagctcgag cgcccgcatg attgagtctt
      121 cggtcggttc ggggatgtgt gcgtggacga gctccatcgc cgattcgcgg agcacgccag
      181 tcggctcgcc gttctcgtcg cgatcgattg atccgccttc cgggtcgcgc gtgtcagccg
      241 tgatcccggc aagctgcaat gcgaacgagt tgaccgccgc gatgtgatgg caggcccggt
      301 acaggaagac ggggtgatct ggtgagaccg catccagatc ggcacgagtc gggtggcgct
      361 ggtcggtcat gcgggcttga tcgtagcctc gtccgatgat ccacgtgccc ggctcctgcc
      421 cattgatccg ccccttgacc gctcgcgcta tgccatcgac cgatccaacc cgttccggcg
      481 tgatatcgac gtcgagcagg gcttgcccga ccatcatcgg atgcgcatgc gcgtcgttga
      541 ggcccggcgt cgcggttcgg ccgcgcaggt cgatcacctc ggcgcgcggg ccggcggcgg
      601 cgcgggcatc cgcttccgac ccaacgtgga cgacgtggcc ggcgcgaatc gccacggccg
      661 aggcgaccgg attcatcggg tcgaccgtca cgacgtggcc attgatcagg acgatgtcag
      721 cggacatgaa agctcccttg gcccggcgtt tacacgccgg cctgatgctg cgtcagccac
      781 caatcggcga tctggtcggc gcgtgcgatc cagacatccc cgagatcgat ggcatagtcg
      841 atgagtcgaa tgatggcgcg ggatggtcca ggccggccgg agacgaacgg gtgcatggtc
      901 aggcacatca tcgacccttc ggaccggatg acgtcgaggt catcccgcca gaactcgaac
      961 gcctgccccg gtgtcaacat cttgtcgacg aagaacgagt agtcgtccca gtgcgccgac
     1021 ggtggcaact ggacgagcgg atcgagcgcg cgatcgggct ggagcagata cgggagatca
     1081 ccgcgcggaa tatccatcac ccagcgcagg ccgagtgcct ggcagatccg gtgcgtgtcc
     1141 gccgtgtatc gccagccggc ggtcttgtga ccggtcggac gttcaccggt aatctccgtc
     1201 agcgtgtccg atgtgcgctc catgtcagtc cgctgctcgt cagggctcat ctcgtgataa
     1261 tagcgatgat cccaggaatg cagcgcgatt tcgtggcctt gctcgaacgc cgcacgcacg
     1321 agctcgggcc gatcctgcgc ttccttgccg acccagcaga acgtggccgg cacatcggca
     1381 tcggagagga tagcgagaat tcgctgggtg ccgaacgggt cgtattctgt ctgcgagatg
     1441 gcatacgtgt tggtcggcgg ctggtagttt gcctcgccat atggcccatc gacgtcgaag
     1501 ctcagacaga gcgcgcaccg gtggccgccc ggccaccggt ctcgcgtgaa tccgtcacgt
     1561 gccatcggag cctccacgac gatctccggg ttattccgct ttcgacgagg cgaggttcgc
     1621 gatcgcggcg acaaggtcgt caacttcctc ttcggtcgtc caccaggcgg ttgagattcg
     1681 ggccgatgcc tgtgccgggc gctggtcaac gaaccggatc gtttgaccgc gctcggcgag
     1741 tcggtcagaa acgtccttcg gatgcatgcc ggcgatattg aagttcacca tgccggccat
     1801 cttccctcca ggggtgagga ctgtgacgcc gtcgatcttc ttgagtgcgt cgaggcaccg
     1861 cttcccgagg gctgcattgc gcgcgacgag ccagtcgaac ccggccttat cgcgcagcca
     1921 ctcgagcccg gtattgaacg cggcgatcgc cgggccgtag gtttcgccca tctcgaaccg
     1981 cgccgctcct ggcggtgggg tgacgtagcc ggcggtgtcg aaggtcccgt accggagata
     2041 gctcgtgcgg aaatcgccga ggcgatcgtc gcgcagaagc atgaatccag agctattcgg
     2101 gccgcagagc catttttggc cggcctggca gtaggcatcg acaccgagcg catggaaatc
     2161 gaccgcgacg tggccggcgc cctgtgcgcc gtcaatgatc gtcaggattc cgcgctcccg
     2221 ggcaaagtcg gcgatctcct tgagcggcat gattgcgccg gtggtccact ggacgtgaga
     2281 gattgcgatc acccgcgtcc ggggcgtaac cgccttcgct agcgcgccga ggacgtcgcc
     2341 gccgccatcg gcaatatcga cggtgcgcac cgtcacgccg aatcgatgtg aaacgcttcc
     2401 gatcgcggaa tagaggcaaa tgtgctcgag ctgggtcgtg atgatctcat cgccggggcg
     2461 ccaaagcacg ccgttcaggg ccgcgttcat gccttcggtg gtgctgcgca tgatggcgac
     2521 ctcggagaca ttggcgttga agatgccggc gatcgtctcg cgaagctggg tggattcatc
     2581 cttgcgcttg ccataaaagg cgagatcgca gcggccggcg ttgagatctt cgatggcctg
     2641 atccacgagc gcctggtgtg cgggaatcgg aactggacca ttcgagccgg cgttgaaata
     2701 ccgggtggca gtgaccgctg gcaactggtt tctcagttca tcgagctttt cgcggtcggg
     2761 aaatgagcca ttggactcca caggagcggt cgcggtctgc ttggtaacca acaagtttct
     2821 ccctggcgat taatgaagga acacacgatc aggcagtcta tggccgctga actacgaggt
     2881 caatcgagcc ggagaggtgc tgtcctcccc ggctcattgc gccgaaaaaa tcaacggcgc
     2941 attagagttt gatcacactc cgcagcgtct caccgcgctc catcgcgtgg aaggcttctt
     3001 ccgattcctc gagggagatc acccgcgtaa ccacgccgtc gaggtcgagc cgtccttgcg
     3061 aataccaggc ggcgaggagc gggaagtcgc gcgttggcag gcagtcgccg taccaggaga
     3121 ctttcagcgc gccgccgaga tcgaagaact tggcgtatgg aagttcgacc atcgcgtcct
     3181 gaccggcgac gccgatgatc acacaggtac cggccaagtc tcgcgcccag agcgccgtca
     3241 taagcgtctc cggcttgccc accgcctcaa atgaggcatt gacgccgaag ccgcccgtga
     3301 tttccttgat cgccgcgacg ggatcgccgt ccttcgggtt gacagtatgg gtcgcgccga
     3361 acccccgggc ccattcgagt ttttggggat cgatgtccac cgcgatgatc gtcgttgccc
     3421 cggccagctt cgcgcccatg atcacggagt cgccgacccc gccgcagccg acgaccgcga
     3481 tcgactggcc cggctgaatg tcggcggtat agagggctgc gccaactccg gtcatgactc
     3541 cgcagccgat gagggacatc tgctcgggcg gcagctcctt gctgaccttc acgcactgct
     3601 ttgcgtggac gagcgtgtgc gtgcagaagg taccgat
//
LOCUS       DASSGX010000513         2666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_855577, whole genome shotgun sequence.
ACCESSION   DASSGX010000513 DASSGX010000000
VERSION     DASSGX010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2666
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855577"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VFI12_09245"
     CDS             <1..405
                     /locus_tag="VFI12_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056637.1"
                     /translation="ELGEMLQESDQELADLLWFAVRDYAMQASDGDLLAEATGHIAEI
                     AFDYDEPTSAAEVWIDFLNWRREPESSSDSEAVLTAYDEIVRAAEMDGAQAVAATYGY
                     QQAQFQKLVDSDDPRATVGNWLPGSDPLETWS"
     gene            532..1149
                     /locus_tag="VFI12_09250"
     CDS             532..1149
                     /locus_tag="VFI12_09250"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HET7056638.1"
                     /translation="MAFELPPLPYANDALEPNIDAKTMEIHHDKHHAAYVNNLNAAIE
                     GNAELGKMSIEDLVKNLSKVPEDKRTAVRNNGGGHYNHSMFWVIMGPNGGGQPTGAFA
                     AAVERDLGGFEAMKEAVNKAGGARFGSGWAWVVLKDGKLSVISTPNQDNPLMDNAGYP
                     ILGVDVWEHAYYLKYQNRRPDYLKAWWNTINWDAVNKRYTDAGGK"
     gene            complement(1198..1698)
                     /locus_tag="VFI12_09255"
     CDS             complement(1198..1698)
                     /locus_tag="VFI12_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056639.1"
                     /translation="MSEIDLENQSVSGRITIPVPARKAFYAGIVAHLQATLPPELREF
                     RHRGDFNLMKVWFEHYRVHYEVVIDQQINKIELGLHFEDGPASSLAFLALLDHRILEI
                     KDRLGPDIELERWTQSWARIYVLRPLTGIDGAVTNECAERLTAMISHLQPIVAESGIP
                     LERPGI"
     gene            1926..>2666
                     /locus_tag="VFI12_09260"
     CDS             1926..>2666
                     /locus_tag="VFI12_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056640.1"
                     /translation="MESTPPTGNPAEESPPVPEQSVAVVDEIVETAPAPPQALSGPNV
                     TIGVPREVVSGERRVALVPDTVKRLVQQGHTVVVASNAGQAAHLLDSAYEIAGATIVP
                     DNAAVFQQAGLIVKVQKPTPDEVSQLQRGQALIAFLQPMVNTDLVQALRDRGVVAFSM
                     DAIPRTSRAQYMDALSSMATVAGYKAVLLAADHLAKFMPLLTTAAGNIPPAKVLVIGA
                     GVAGLQAIATARRLGAVVEAYDTRPVVKE"
BASE COUNT          515 a          839 c          865 g          447 t
ORIGIN      
        1 gagctcggag agatgctgca ggagtccgat caagagctgg cggacctgct ctggttcgcg
       61 gtccgcgact acgcgatgca ggcttccgac ggagacctcc tcgccgaggc aacggggcac
      121 atcgccgaaa tcgcattcga ctatgacgag ccaaccagcg ccgccgaagt ctggatcgac
      181 ttcctcaact ggcgtcgcga gccggaatcc tcatccgatt ccgaggcggt gttgaccgcg
      241 tatgacgaga tcgtccgcgc cgccgagatg gatggggcgc aggcggtggc ggcgacgtac
      301 ggataccagc aggcccagtt ccagaagctg gtcgattccg atgatccccg ggctacggtc
      361 gggaactggc tgcccggtag cgatccgctc gagacctggt cgtagcctgg attcaccgca
      421 atcgcccgga aatgcggtgg tatttggtaa cctaggcgac gttagcgatc gtcgcttggc
      481 cacgggtgcc gggcttggac aggtctctcc gaagagaaag gattggtcag catggccttt
      541 gagcttcccc cgcttccata tgccaatgac gcgctcgagc cgaacatcga cgcgaagacg
      601 atggagatcc atcacgacaa gcaccatgcg gcgtacgtga acaacctcaa cgccgcgatc
      661 gagggcaacg ccgaactcgg caagatgagc atcgaggatc tcgtcaagaa tctcagcaag
      721 gtgcccgaag acaagcgcac ggcggtccgc aataacggcg gcggccatta caaccacagc
      781 atgttctggg tgatcatggg cccgaacggc ggtggtcagc cgaccggcgc gttcgccgca
      841 gcggtcgagc gggatctcgg cggattcgaa gcgatgaagg aagccgtcaa taaggccggc
      901 ggcgcccgct tcggctccgg ctgggcgtgg gtcgtcctca aggatggcaa gctctcggtg
      961 atcagcacgc cgaatcagga caacccgttg atggacaatg caggctatcc gatcctcggc
     1021 gtcgacgtct gggagcacgc ctactatctg aagtatcaga atcgccggcc ggattacctc
     1081 aaggcctggt ggaacaccat caactgggat gccgtcaaca agcgatacac ggacgccggc
     1141 ggcaaataac caagctcagc aactcgactg gagacccggc cgcatggccg ggtctcgtca
     1201 tatacccggt cgctcgagcg ggatccccga ttccgcgacg atcggctgca agtgactgat
     1261 catcgcagtc aggcgctcag cgcactcgtt ggtcactgcg ccgtcgatcc cggtcagcgg
     1321 tcgcagcaca tagatccgcg cccacgactg cgtccaccgc tccagctcga tatcaggacc
     1381 caagcgatcc ttgatctcaa gaatccggtg gtccaggagc gccaggaacg ccagcgagct
     1441 cgcgggaccg tcctcgaagt gcaagccgag ctcgatcttg ttgatctgtt gatcgatgac
     1501 gacctcgtaa tggacgcggt agtgttcgaa ccagaccttc atcaggttga agtcgccgcg
     1561 atgacgaaac tcccgcaact cgggcgggag cgttgcctgg aggtgcgcaa cgatgccggc
     1621 atagaacgcc tttcgggcag ggacgggaat cgttatccgg ccggaaacgg attggttctc
     1681 gaggtcaatt tcgctcatat cggtacatga tcgcgcacga gcgacgctgc ggaacaggcc
     1741 gcctggggct cgtccgccgg agtccgccag tttcgtatac catttgatct cgaaattatg
     1801 cccggcggag gcgccgggcg ggcaatggcc gcggcactgt ccggtcggcc gtttgtcttg
     1861 tgtgcggtca gggcaactcg gggacatcat gtgtcgccga atttccccat ggagagtgga
     1921 gcgagatgga gagcacgccg ccgaccggaa acccggcgga ggaatcaccg cctgtgcccg
     1981 agcagagcgt tgcggttgtg gacgagatcg tcgagaccgc gccggcgcca ccccaggcac
     2041 tgagcggccc gaatgtgacg atcggagtgc cgcgggaggt cgtctccggc gagcgccggg
     2101 tcgcactcgt gcccgatacc gtgaagcggt tggtccagca ggggcacacg gtggtggtcg
     2161 cgtcgaatgc cggccaggcc gcccatctgc tcgacagcgc ctatgagatc gccggcgcca
     2221 caatcgtccc ggacaacgcc gccgtcttcc agcaggccgg gttgatcgtc aaggtccaga
     2281 agccgacgcc tgacgaggta tcgcagctcc agcgcggaca ggccctgatc gccttcctgc
     2341 agccgatggt caataccgat cttgtccagg cgctccgcga ccgtggcgtc gtcgccttca
     2401 gcatggacgc gatcccgcga acgtcccgcg cccagtacat ggacgccctc tcctcgatgg
     2461 cgaccgtggc cggatacaag gctgtgctgc tggcggcaga tcacctcgcc aagttcatgc
     2521 cgctgctgac gacagccgct ggcaacatcc cgccggccaa ggtcctggtg atcggcgccg
     2581 gtgtcgccgg gctgcaggcg atcgcgaccg cccgccgcct cggcgcggtc gtcgaggcgt
     2641 atgacacccg gccggtcgtg aaggag
//
LOCUS       DASSGX010000514         2911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_635689, whole genome shotgun sequence.
ACCESSION   DASSGX010000514 DASSGX010000000
VERSION     DASSGX010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_635689"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1541)
                     /locus_tag="VFI12_09265"
     CDS             complement(<1..1541)
                     /locus_tag="VFI12_09265"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET7056641.1"
                     /translation="MPERQADAVIIGGGLGGCAAALAALRFGRRVFLTEETDWLGGQL
                     TAQAVPPDEHPWIEQVGCPPSYRRLRDGIRDYYRRHYPLTSIARADPFLNPGQAEVSP
                     ICHEPRVSLAVLEAMLQPWRSGRRLDVLTEHQPIAADVDGDLVRSVICRSLANGDEVT
                     LHAPYIVDATEFGELLELVGVEHVIGAESRDETGELHALPGPAQPLDQQAVSWCFAVD
                     FRSGEDHTIDQPADYRFWRDYAADFWPGKQLSWTDWHPETLQKRYRPIFDGPMERARA
                     DDLWHFRRILYGGHYPPGTFESDITLVNWPQIDYWLGPLIGVSAEKRDRHLRGALQLA
                     LSMLYWMQTEAPRLEGGYGYPELRLRPDIVGTADGMAKHVYVREARRIRAEFTIREQH
                     VGVEARGPRTGAELFDDSVGIGSYRIDLHPSTAPRNYVDVSSFPFQIPLGALIPQRVE
                     NLLPACKNIGTTHITNGCYRLHPVEWNIGEVVGALIGWCLEHRLTPRQVRNDRERLAD
                     FQRVLT"
     gene            1596..2567
                     /locus_tag="VFI12_09270"
     CDS             1596..2567
                     /locus_tag="VFI12_09270"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET7056642.1"
                     /translation="MNRRSLLTSTLAFPAALANRESPTRAEDAAARFQYPIAWAGQRP
                     GNGFFIRHGYACENVWYNPGFLHTGEDWYAIDDRETAGADVSAIAQGTVVFADSDYPG
                     RVVIVQHEPELFSMYGHLDYALTVSEGDAVTMGQRLGTVLLRTDGRAPSHLHFEVRTF
                     LTTPEVNGDQPRYGFGCGLNCPPGPGYWPITAPEHPSAIGWRNPTHVIARRMFGRGPI
                     PAGFDVVVPDGVNEDAPVWSLPADRQHAEQVGEPRLAAGERFPLLQVAAGPEATNGWS
                     AERYRLWYRIEIETAEPGWIQAAVPSNQETGNDGRSSSLQSVLIPAG"
     gene            complement(2564..>2911)
                     /locus_tag="VFI12_09275"
     CDS             complement(2564..>2911)
                     /locus_tag="VFI12_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056643.1"
                     /translation="GRWRFEGTTIPIAAIRSDLRYGRTATKAQYRFMDLTDDEIDAAL
                     AFEYPAVRPSSLDVEYASVIVQCVCGEDTSVTSLAPRAEVVECICGRTWRIVINSEQI
                     RRIAAEEPGSRPH"
BASE COUNT          485 a         1022 c          931 g          473 t
ORIGIN      
        1 gtgagcaccc gctggaaatc ggcgaggcgc tcacggtcgt tccgaacctg ccgcggcgtc
       61 aatcgatgct ccaggcacca gccaatcagc gcgcccacca cctcgccgat gttccactca
      121 accggatgca ggcggtagca gccgttcgtg atgtgcgtcg tgccgatgtt cttgcacgcc
      181 ggcagcagat tctcgacgcg ctggggaatc agcgcaccga gcgggatctg gaaggggaag
      241 ctgctgacgt ccacgtaatt ccggggagcg gtgctcgggt gcagatcgat ccggtagctg
      301 ccgatcccga cgctgtcgtc aaataattcc gcgccggtcc gcggcccccg cgcctccacg
      361 ccaacatgct gctcgcgaat ggtgaactcc gcccggatgc gccgggcctc gcggacatag
      421 acgtgcttcg ccatgccatc cgcggttcca acgatatccg ggcggaggcg caattccgga
      481 tagccgtatc cgccttcgag ccggggcgcc tctgtctgca tccaatagag catggacagg
      541 gcaagctgca gcgcgccgcg caggtggcga tcgcgcttct cggcgctgac gccgatcagc
      601 gggccgagcc agtagtcgat ctggggccag ttgacgaggg tgatgtcgct ctcgaacgtc
      661 ccgggcgggt aatgaccgcc atacaggatg cgccggaagt gccacaggtc gtccgcgcgg
      721 gcccgctcca tgggcccgtc gaagatcggc cggtagcgtt tctgaagcgt ctcgggatgc
      781 cagtcggtcc agctcaactg cttgccgggc cagaagtcgg cggcatagtc gcgccagaat
      841 cggtaatccg caggctgatc gatggtgtgg tcctcgccgg accggaaatc gaccgcgaag
      901 caccatgaca ccgcctgctg atcgagcggc tgggccggac cgggcagtgc gtgcagctcc
      961 ccggtctcgt cccggctctc cgccccaatg acatgctcca cgccaaccag ctcgagcaac
     1021 tccccgaact cggttgcgtc gacgatgtac ggcgcatgga gcgtgacctc atcaccattc
     1081 gccaggctcc ggcagatcac cgatcggaca aggtcgccat ccacatcggc cgcaatcggc
     1141 tgatgttcag tcaacacgtc gagccgcctg ccgctccgcc acggctgcag catcgcctcc
     1201 agcacggcaa ggctcacccg cggctcatgg cagatcgggc tgacctccgc ctggccggga
     1261 ttgaggaacg ggtcggcgcg ggcgatcgag gtcagcggat agtgccggcg gtaatagtca
     1321 cggattccat cgcgcaggcg gcggtagctc ggagggcacc cgacctgctc gatccacggg
     1381 tgctcatccg gcggcacggc ctgggccgtc aattgcccgc caagccagtc ggtctcctcg
     1441 gtcaaaaaga cccgccgccc gaaccggagc gcggccagcg ccgccgcaca cccgcccagc
     1501 ccgccgccga tgatgaccgc atccgcctgt cgctccggca tcgtatcccc gtccgctgcg
     1561 caaaccccac gtcccggcca atcgtaggac ctgacatgaa ccgacgctcg ctgcttacgt
     1621 ccaccctggc attccccgcc gccctcgcca accgggagtc gcccacccgg gcggaggacg
     1681 ctgcggcgcg gttccagtac ccgatcgcat gggccggtca acgtcccggg aacggattct
     1741 tcattcgcca tggctatgcc tgcgagaacg tgtggtacaa ccccggcttc ctgcacaccg
     1801 gcgaagactg gtacgcgatc gatgatcggg agacggccgg cgcggatgtc tccgcgatcg
     1861 ctcaggggac cgtcgttttc gccgattccg actaccccgg ccgtgtggtc atcgtgcagc
     1921 acgaacccga gctcttttcg atgtacgggc acctggacta cgcccttacc gtgagcgagg
     1981 gcgacgccgt cactatgggg cagcgcctcg gcacggtcct gctccggacc gacggccgag
     2041 ctccgagcca cctccacttc gaggtccgca ccttcctgac cacgcccgag gtcaacggcg
     2101 accagccccg ctacggcttc gggtgcgggc tcaactgccc gcccgggccc ggctattggc
     2161 cgatcaccgc gccggagcac ccgtcggcaa tcggctggcg caatccgacg cacgtcatcg
     2221 cccggcgcat gttcggtcgt ggcccgattc cggccgggtt cgacgtagtc gttcccgacg
     2281 gcgttaacga agacgcccct gtctggtcgc tccccgctga ccgccaacac gctgagcagg
     2341 tcggggaacc gcggctcgct gcgggtgaac gatttccgct gctccaggtc gccgccggcc
     2401 cggaagcgac caatggctgg agtgcggagc gataccggct ctggtatcgc atcgaaatcg
     2461 agacggcgga accgggctgg attcaggctg ccgtgccgtc caaccaggag acgggcaacg
     2521 acggccggtc gtcatcactc caatccgtgt tgattccggc gggctagtgc ggacgtgaac
     2581 cgggttcctc cgcggcgatg cgccggatct gctcggaatt gatcacgatt cgccatgtgc
     2641 gcccgcagat gcactcgacc acctcggcgc gtggcgccag cgacgtcact gacgtgtctt
     2701 cgccgcagac acattgcacg atgaccgacg catactcgac gtcgaggctc gacgggcgca
     2761 cggccgggta ctcgaacgcc agggcggcat cgatctcgtc gtcggtcagg tccatgaatc
     2821 ggtactgggc cttggtcgcg gtgcggccat agcggagatc gctgcgaatc gcggcgatcg
     2881 ggatcgtggt tccctcgaac cgccaccggc c
//
LOCUS       DASSGX010000515         2163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1070359, whole genome shotgun sequence.
ACCESSION   DASSGX010000515 DASSGX010000000
VERSION     DASSGX010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1070359"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /gene="ruvB"
                     /locus_tag="VFI12_09280"
     CDS             complement(<1..652)
                     /gene="ruvB"
                     /locus_tag="VFI12_09280"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HET7056644.1"
                     /translation="MTAQPPTPADPDSRLVGAGQQQTDVVSEMSLRPRRLSEYIGQDR
                     VKSTLNVFIQAARRRGETLDHVLLYGPPGLGKTTLSAIVAAEMGVNLRITAGPAIERA
                     GDLVSILTNLKAGDVLFIDEIHRLSRQVEEVLYPAMEDYAVDIVIGKGPSARTMRIKL
                     PRFTLVGATTRLALLTGPLRDRFGAVLRLDYYDEPAMEEIIRRSAKILAIEIDDCGT"
     gene            complement(696..1280)
                     /locus_tag="VFI12_09285"
     CDS             complement(696..1280)
                     /locus_tag="VFI12_09285"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7056645.1"
                     /translation="MTFNMLAIVVVLAMVVGAVGVAVFDGLLSGGSNDKPLTVDPAES
                     DPVEQQYRDKIAKDPNDVAAMSALANYLGNTGKIEEAITWYEKALTITPDDMDLRLDF
                     ASALASGGKMRDSELQYKKVVAAQPQNAFAMLGLARLYRSWSPPRLTEAVTFYQQAID
                     AAGDSVVRDVAREELSAITGTPVASPGASPSPAP"
     gene            1479..1551
                     /locus_tag="VFI12_09290"
     tRNA            1479..1551
                     /locus_tag="VFI12_09290"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1512..1514,aa:Thr,seq:cgt)
     gene            1583..1664
                     /locus_tag="VFI12_09295"
     tRNA            1583..1664
                     /locus_tag="VFI12_09295"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1617..1619,aa:Leu,seq:cag)
     gene            2089..2161
                     /locus_tag="VFI12_09300"
     tRNA            2089..2161
                     /locus_tag="VFI12_09300"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2122..2124,aa:His,seq:gtg)
BASE COUNT          416 a          651 c          714 g          382 t
ORIGIN      
        1 tggtgccgca gtcatcaatc tcaatggcga gaatcttggc tgagcgacgg ataatctctt
       61 ccatcgccgg ttcgtcgtaa taatcgagcc gcagcaccgc accgaaccgg tcgcgcagcg
      121 gcccggtcag caacgcgagc cgggtcgtcg cgccgaccag cgtgaagcgc ggcagtttga
      181 tccgcattgt ccgcgccgac gggcctttac cgatcacgat atcgaccgca tagtcctcca
      241 tcgccgggta aagcacttcc tcgacctgac gggagagtcg gtggatctca tcgatgaaga
      301 ggacgtcgcc ggccttgagg ttggtgagaa tcgagacgag gtcgcccgcg cgctcgattg
      361 ccggaccagc cgtgatccgc aggttcaccc ccatctccgc cgcgacgatt gccgagaggg
      421 tcgttttgcc gaggccgggc ggaccataga ggagcacgtg gtcgagcgtc tctccgcgcc
      481 ggcgagcggc ctgaataaag acattcaggg tcgatttgac gcgatcctgc ccgatgtact
      541 cgctcaggcg acgcgggcgc aggctcatct cggacacgac gtcagtctgc tgctgaccgg
      601 cgccaacgag gcggctatcg gggtcggcgg gagtgggcgg ttgcgctgtc atgacgatga
      661 gtataccgcg aatagaacaa gcgttcagcc tggaatcagg gcgccggcga agggctggcg
      721 ccgggcgacg caaccggtgt gcctgtgatc gcggacagct cctcccgggc gacatcgcgc
      781 acgacggagt cgccggccgc gtcgatcgcc tgctggtaga aggtcactgc ctctgtgagg
      841 cgcggcggcg accagctgcg atagagccgg gcgaggccaa gcattgcgaa cgcgttctgg
      901 ggctgagccg cgacgacctt cttgtactgc agctcggaat cacgcatctt gccgcccgag
      961 gcaagcgcgc tcgcaaaatc gaggcgcagg tccatatcat ccggtgtgat cgtgagcgcc
     1021 ttctcgtacc aggtgatcgc ttcctcgatc ttgccggtgt tgccgaggta gttggccagg
     1081 gccgacatcg ccgccacgtc gttgggatct ttcgcaatct tgtcccggta ttgctgctca
     1141 accggatcgg actcggccgg gtcgaccgtc agcggcttgt cgttactgcc accgctgagg
     1201 agaccgtcga agaccgccac gccgacggct ccgacaacca tcgcgagcac gaccacgatg
     1261 gcgagcatgt tgaacgtcat gatcgagcgc gatcgcttcg gcgcgcttcg cttgcgtctt
     1321 ggattggatc ggggtcggtg tgtcattcgc cagcaatgat tgcgtgaaat cagcatcgaa
     1381 cagtaccata ggtgggcgga ctacctgttg gtcgcccgcg ttccgcgttg atcaaatgcg
     1441 ggcgggctga taaaattaag agccgtgcaa tagcacgcgc cgacctagct cagcaggtag
     1501 agcagctgtt tcgtaaacag caggtctggg gttcgagtcc ccaggtcggc tccggggcga
     1561 cgaagagtcg cctctattgc aggccgaagt ggcggaatcg gcagacgcgc tacgttcagg
     1621 gcgtagtggg gagacccgtg ggagttcaaa tctccccttc ggcaccgtga tccggcggct
     1681 cttcggggcc gcccgcacgt tgacaagcga atgagaaggg cgaaaggatg gtcgcgggca
     1741 gtggcacgcc gctgttcgag gaaagtccga gctgcgcaga gtgaaggtgc ctggtgaaag
     1801 ccagggaggc aggcggcagc ttgtcgacag tccagatcgg cgcgccggga aaccggtgcg
     1861 agagcaacag caaccaaacc gggcgcggca acgcgtaccg gctgaaaagg gcaatcctcc
     1921 ctgcagcaat ctccgatccg accgttatca ggtggctcgc cgaggtcgaa agtcgagagc
     1981 agccgtcacc cgcgagggcc ggccggtcat acgaccgaga gagatgacca tcgtaaacag
     2041 aactcggctt acgtttcgcc ccgctcgctt gtcaaacaac cccatacggt gcctttagct
     2101 cagtcggcag agcgcttgtc tgtggaacaa gaggtcacgg gttcgagccc cgtaaggcac
     2161 ccc
//
LOCUS       DASSGX010000516         2598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_526439, whole genome shotgun sequence.
ACCESSION   DASSGX010000516 DASSGX010000000
VERSION     DASSGX010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526439"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="VFI12_09305"
     CDS             complement(<1..662)
                     /locus_tag="VFI12_09305"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET7056646.1"
                     /translation="MTPVSLFLVLVAAFVLMKIQLVVVLVVLAVLFATIIERPVMALH
                     KRRVPRPLSILAVYIMIIGGIVLAGLLAAPLISDEAQNFRDEAPTQLRELKTRWELGG
                     NAALKGPGVNVLDRLLAEIDNPSSPSEDVTVGIVTGVGGGLVGLLSVFVMAFYYLTEK
                     ALLRRIIINQIRPSSRERVERVWDQVEAQVGRWLRGQLTLCLIIGSASFIGYGLMGVR
                     FWP"
     gene            complement(753..1133)
                     /locus_tag="VFI12_09310"
     CDS             complement(753..1133)
                     /locus_tag="VFI12_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056647.1"
                     /translation="MRPSNVLIPKDRMNATTPMRCPICDGPLERVLIRDMGGITAGII
                     WQLHAGRCPQHGWFQAEVVGRPPSEIFPVERPFGVAREIDIDGQPVFAFTTVWNDQPA
                     EVRLRKDADPFDRRYWKVRLATRR"
     gene            1340..1795
                     /locus_tag="VFI12_09315"
     CDS             1340..1795
                     /locus_tag="VFI12_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056648.1"
                     /translation="MPDLDLIDRLVATYRILNHTVRLKPEADLVQGNPSTKAIIRGMR
                     DEELRFSQELKSRISGQQIEIVESADQAGLHSTESPETTAALIAQFGTARESTLSMLR
                     TLPDDQWDVTGDYPRSIRTDLISLIDRDRTVLDTIGKTLGEPLVEAATV"
     gene            1808..>2598
                     /locus_tag="VFI12_09320"
     CDS             1808..>2598
                     /locus_tag="VFI12_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056649.1"
                     /translation="MSLRRQHLSPVDGFDNRIDWQAAIDRWNQRAPRWALGPGLLSFH
                     LTLFGLAFLATLTWNLIADPADLGMIDLFRYWGVLAVVHTILVGGWMIGWRLLRLGEP
                     EPRRFTIPAAALPQLRSNQSRTPAWQTAWNRSNAAASRVNTTARRWAGAPSRRAETQA
                     PPRDAATGWPEQPPIFREAVAGAETTEPPAVDVGAATWPNSAPLSTTLSGSAASNPAD
                     ELVSVDHRDEEGEDDQARAWVDGFVESRSKDKDQRWSWVEAAAAA"
BASE COUNT          448 a          915 c          810 g          425 t
ORIGIN      
        1 ggccagaagc gcacgcccat cagcccatag ccgatgaacg aggccgagcc gatgatcaag
       61 cagagcgtga gctgtccccg cagccagcgg ccaacctgcg cttcgacctg atcccagacc
      121 cgctcgaccc gctcccggga cgacggcctg atctggttga tgatgattcg ccgcagcagc
      181 gccttttcgg tcaggtagta gaaggccatc acgaagaccg acaacagccc gaccaggccg
      241 ccgccgacgc cggttacgat gccgaccgtc acgtcttcag acgggctcga ggggttatcg
      301 atctcggcca ggaggcgatc gagcacgttg acgccgggac ccttgagcgc cgcgttgccc
      361 ccgagctccc agcgtgtttt caactcgcgg agttgcgtcg gcgcctcatc cctgaagttt
      421 tgcgcctcat cggaaatgag cggcgccgcc agcaatccgg cgaggacgat gccgccgatg
      481 atcatgatgt agacggcgag gatgcttagc ggccggggca cgcgccgctt gtgcagcgcc
      541 atcaccggcc gctcgatgat cgtcgcgaag aggaccgcca gcacgaccag caccacgacg
      601 agctggatct tcatcagcac gaacgccgcg accagcacga ggaacagcga aaccggcgtc
      661 aatcccggac gtcgccattc gctgtccgcg ggcgcggatg atggtggttc aggagcgcgt
      721 tgctgcgggc ggtcagtcac tgatgtttgc gcttacctgc gagtggcgag gcggactttc
      781 cagtaccgcc ggtcgaatgg gtccgcatcc ttgcgcagcc tcacctcggc tggctgatcg
      841 ttccagaccg tcgtgaacgc gaaaacgggt tggccgtcga tatcgatctc gcgagccacg
      901 ccgaacggtc gctcgaccgg gaagatttcg ctcggcggcc gaccgacgac ttcagcctgg
      961 aaccagccat gctgcgggca ccgcccggcg tgcaactgcc agatgatgcc ggcggtgatg
     1021 ccgcccatgt cacgaatcag cacccgctcg agcggcccat cgcaaatcgg gcaccgcatc
     1081 ggcgtggtcg cattcatgcg atcctttgga atcaggacat tggatggtct cacgcagttg
     1141 ctccaaccag aaccaggcag gctccgccaa agcggcggtt tcgcccacga gtctagcatt
     1201 cagcgatgcc cttcgcgact ccaggtgcgc catcgcgact gcgcagattt acgcagattt
     1261 ggtgtactct gtatgcgaga cgacctgcgc cccgatgcta tcctgaggtc gtttcgctga
     1321 agttggaggg tcccgcctta tgcccgatct ggatctgatc gatcgtctcg tcgctactta
     1381 ccgcatcctc aaccacaccg ttcgcctcaa gcccgaggcc gacctcgtcc agggcaaccc
     1441 ctcgaccaag gccatcatcc gcggaatgcg cgacgaggag ctccgcttct cgcaggagct
     1501 caagtcccgc atctccggcc agcagatcga gatcgtcgag agcgccgacc aggccggctt
     1561 gcattccacc gagagccccg agacgaccgc cgccctgatc gcccagttcg gcactgcccg
     1621 cgaatcgacc ctctcgatgc tgcggacgct ccccgacgac cagtgggatg tgaccggcga
     1681 ctatccgcgc tcgattcgca ccgatctgat cagcctgatc gaccgcgacc ggacagtgct
     1741 cgacaccatc ggcaagacgc tcggcgagcc gctcgtcgaa gcagcgacgg tctagggagg
     1801 cccgcccatg tccctccgcc ggcagcacct ttcgcccgtc gacgggttcg acaaccggat
     1861 tgattggcag gcggcgatcg atcgctggaa tcagcgcgcc ccgcggtggg cgctcgggcc
     1921 cggcctcctc tcgttccacc tgacactctt cggcctggcc ttcctggcca ccctgacctg
     1981 gaacctgatt gccgacccgg ccgacctcgg tatgatcgat ctcttccggt attggggcgt
     2041 gttggcggtc gtccacacga tcctggtcgg cggctggatg atcggctggc ggctgctcag
     2101 gctgggtgag cccgagccgc gccgcttcac gatcccggcg gcagccctgc cgcagttgcg
     2161 ctcaaatcag tcccgcactc cggcttggca aaccgcgtgg aatcgcagca atgccgcggc
     2221 ttcccgggtc aacacgaccg cccgccgctg ggccggcgca ccgtcccggc gcgccgagac
     2281 gcaagcgccg ccgcgcgacg ccgccaccgg ctggcccgag caaccgccga tcttccgcga
     2341 ggccgttgcc ggggcagaga ccaccgagcc accagcggtc gacgtcggcg cggccacctg
     2401 gcccaattcg gccccgctct cgaccaccct ctccggcagc gccgcttcga acccggcgga
     2461 cgagctcgtc tcggtcgatc accgggacga ggaaggcgag gatgaccagg ccagggcgtg
     2521 ggtcgacggt ttcgtcgaat cgcgttcgaa ggacaaggat cagcgctggt cgtgggtcga
     2581 ggccgcggcc gctgcctg
//
LOCUS       DASSGX010000517         2921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_856421, whole genome shotgun sequence.
ACCESSION   DASSGX010000517 DASSGX010000000
VERSION     DASSGX010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856421"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1190)
                     /locus_tag="VFI12_09325"
     CDS             complement(<1..1190)
                     /locus_tag="VFI12_09325"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7056650.1"
                     /translation="MRNRRPVFALIVANAVSLVGSSLTGIAVPWFVLETTGSAGKAGL
                     TGAFAFLPAFFAGIFGGAVVDRIGARHSAMVADLVSGTAILMVPVLYHTVGLQFWQLL
                     ILVMIGSLLDIPGLTARRALLPELATLGDVRLERVNAMLEGNWQLSILLGPPIAGVLI
                     GFMGAANVLWIDAASSLISFTALLLLIPANIHERARAASQGYLRDLRAGIQFLWRDQV
                     LRWISIMLAISNAFGAPVFSLIMVVYIKGRFDDPRYLGVMLSASGIGLMIGNAFYGWR
                     GFRLPRRWVVTMFLITYTLVIWPFAFTFPFPVLLVLVALSGLFDGPVNPLLVTVRLER
                     IPVELRGRVFAATSAMAQAFPAFTIPLAGWAIEGVGLTTTVLIIAVGAQVAGLLLTVL
                     PAWA"
     gene            complement(1187..2203)
                     /locus_tag="VFI12_09330"
     CDS             complement(1187..2203)
                     /locus_tag="VFI12_09330"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HET7056651.1"
                     /translation="MSFDLYRLPRFPLANLPTPLQEASRLREALGGPAKCPRILIKRD
                     DLTGLAFGGNKVRKLEMLVGDALVRGADTLITTGAVQSNHARATAAAAVVAGLRAILV
                     LESADMCPPVQGNLLLDRLLQADVRFVPKDGDTAKAMETVADEVRAAGGNPYVIPIGG
                     SSPSGAAGYLTMTLELKVQLAAMGASPSRLYFASGSRGTQAGIVLGARVFDMPYEIYG
                     VLVSKSRPETEARALSIANEAAELVGSDVRLNSADLANVDGYFGEAYAIPTPEGDAAV
                     LLLARTEAIFLDPTYTGKSMSALIDHIQTGQLGPDETVVFIHTGGTPAIFAHADRLSE
                     LAKA"
     gene            complement(2211..2699)
                     /locus_tag="VFI12_09335"
     CDS             complement(2211..2699)
                     /locus_tag="VFI12_09335"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET7056652.1"
                     /translation="MTSEGAYIADQLIRQITLVRRELSDLPDDALNEVLTIQPTNTLF
                     QLGTHIAGSARYWAITNTGGTDFHRDRDAEFMAVGNSADLLANLDELIGQIGDHVRGL
                     TAEQLNRACSLRTASFSFWEGHEPLPQRHALLHALEHTGLHLGHIQITRQILGFDPVE
                     EE"
BASE COUNT          558 a          929 c          984 g          450 t
ORIGIN      
        1 tgcgcccagg cggggaggac ggtcaacagt agcccggcaa cctgcgcgcc gacggcgatg
       61 atgagaacgg tcgtagtcag gcccactccc tcgatcgccc agccggcaag cgggatggtg
      121 aatgccggaa aggcctgcgc catggccgag gtggccgcga aaacccggcc gcgcagctcg
      181 accggaatcc gttcgagccg aaccgtcacc agcaagggat tcaccggtcc gtcgaagagg
      241 ccactgagcg ccacaagcac gagcagaacc gggaacggga aggtaaaggc gaacggccag
      301 atgaccagcg tgtaggtgat caggaacatg gttaccaccc atcgcctggg cagccggaag
      361 ccccgccaac cgtagaaggc gttgccgatc atgagcccga tgcccgaagc cgacaacatg
      421 acccccaggt agcgtggatc gtcgaagcgg cccttgatgt agaccaccat gatcagcgag
      481 aaaacgggcg cgccaaacgc gttgctgatc gccagcatga tcgaaatcca gcgcaagacc
      541 tgatcgcgcc agaggaactg gattccggcg cgcaggtcgc ggagatatcc ttgcgacgcc
      601 gctcgcgccc gttcgtggat gtttgccgga atcagcagca ggagcgcggt aaatgagatc
      661 agggacgatg ccgcgtcgat ccagagcacg tttgccgcgc ccatgaagcc gatcaggacg
      721 ccggcgatcg gcggcccgag caggatcgag agttgccagt tgccctcgag catggcgttg
      781 actcgctcca ggcgcacgtc gccgagcgtg gccagctccg gcaaaagggc acgcctcgct
      841 gtcagtcccg ggatatcgag caacgatccg atcatcacga ggatcagcag ttgccagaac
      901 tggagcccga ccgtgtggta gagcaccggc accatcagga tggcagtgcc gctaaccagg
      961 tccgcaacca tcgcgctgtg ccgggcgccg atccgatcaa cgaccgcacc gccgaagatc
     1021 ccggcaaaga aggccggcag gaacgcgaac gcgccggtca gccctgcctt cccggcgctc
     1081 ccggtcgtct ccagcacgaa ccacggaacc gcgattccgg tcagggagct gccgaccagt
     1141 gagaccgcat tggcgacgat cagcgcgaag accggccgcc gattcctcac gctttggcaa
     1201 gctccgagag ccgatccgcg tgcgcgaaga tggccggcgt gccgcccgtg tgaatgaaga
     1261 cgaccgtctc gtcaggcccg agctggccgg tctggatgtg gtcgatcagg gccgacatcg
     1321 atttcccggt gtaagtcggg tcgaggaaga tcgcctcggt ccgggccagc agcagcaccg
     1381 ccgcatcacc ttccggtgtc ggtatggcat acgcctcgcc gaagtatccg tcgacattcg
     1441 caaggtcggc tgagttcagg cggacgtcgc tcccgaccag ctccgccgct tcgtttgcaa
     1501 tgctgagcgc gcgggcctca gtctccggac ggctcttcga gacgagcacg ccgtaaatct
     1561 cgtacggcat gtcgaagacg cgcgcgccga gcacgatgcc ggcctgcgtg ccgcgcgagc
     1621 cgctcgcgaa atagagccga gatggcgacg cacccatggc ggcgagttgc accttgagct
     1681 ccagggtcat ggtcaggtag ccggccgcgc cggaggggct ggagccgccg attgggatca
     1741 cgtacgggtt cccgcccgca gcccgcactt cgtcggcgac ggtctccatg gccttggcgg
     1801 tatcgccatc cttgggcacg aagcgaacgt ccgcctgaag caaccggtca aggagcagat
     1861 tgccttgcac gggtggacac atgtcggcgg actcgagcac gaggatcgcc ctcagcccgg
     1921 cgacgacggc agccgcggcg gtggcgcgag cgtggttgga ctggaccgca ccggtcgtga
     1981 tcagcgtgtc ggcgccccgg acgagggcgt cgccgacgag catctcgagc ttgcggacct
     2041 tgttgccgcc gaacgcaagg ccggtcaggt cgtcgcgctt gatcaggatg cgcgggcact
     2101 tggcgggacc gccgagggcc tcacgaaggc gcgaggcttc ctggagcggg gtcggcaggt
     2161 tggcgagcgg aaaccggggc aaacggtaaa gatcgaacga cacgggtggc ctactcctct
     2221 tcaaccggat cgaacccaag gatttgtcgc gttatctgaa tatgtccgag gtgcagcccg
     2281 gtgtgttcca gcgcgtgcag cagggcatgc cgctgcggga gcggttcgtg cccctcccag
     2341 aacgagaagg atgcggtccg cagcgagcat gcccgattga gctgctccgc ggtcagtccg
     2401 cgcacgtggt cgccgatctg cccgatcagt tcgtcgaggt tggcaagcag atccgcgctg
     2461 ttgccgaccg ccatgaactc ggcatcgcga tcgcggtgga aatcggtgcc gccggtgttc
     2521 gtgatcgccc aataccgggc cgagccggcg atatgtgtgc cgagctggaa gagggtattg
     2581 gtcggctgga tcgtcagcac ctcgttgagg gcgtcgtcgg gcagatcgct cagttcgcgg
     2641 cggacgagcg tgatctggcg gatgagctga tcggcgatat acgcgccctc ggaagtcatg
     2701 acccaggacc tttcgctctt gacgaatatg tggtcgttcg ctacgctgac ggcatgattg
     2761 cagaagaagc cgctcgagac cgaatcgccg caaccgcaaa tgccctgggg gacgataccc
     2821 gattgcgcct gctgatgttg ctcctgtccg cgggaagcgg tggcatgacc gtcaccgacc
     2881 tggcggagcg cctgagcctg gcccaaccgc gcatctccac c
//
LOCUS       DASSGX010000518         3710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_965730, whole genome shotgun sequence.
ACCESSION   DASSGX010000518 DASSGX010000000
VERSION     DASSGX010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965730"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VFI12_09340"
     CDS             <1..225
                     /locus_tag="VFI12_09340"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET7056653.1"
                     /translation="DHAVQSALDAIQVHGANGYSNEFPVERYLRNSKAAVIYEGTSQL
                     HTLVQADYALGYREDRPLRCEPWPADGSTT"
     gene            742..1623
                     /locus_tag="VFI12_09345"
     CDS             742..1623
                     /locus_tag="VFI12_09345"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7056654.1"
                     /translation="MSTPREAGGQPDLEARDVSKRYGHGVVALEHLDIAIAPSTTTAL
                     VGPNGAGKSTLMKLWMGFERPTSGSLSVRGWDPWRQRAHAVAVLGYVPQSVSLYRDLT
                     IGDHLDLALSLRPTFDRPYATARLARLGITTQRDAGELSGGEQSQVALALALGTRAPV
                     LLLDEPLASLDPLARREFLAVVGDVARTEGVTVVLSSHIVTDVESSCERIVVLGAGRV
                     LVNDSVAMLLASHLIRVDNGVAGPHVATCIGGFPGPAGEALTLWQTSGPAVSGYRRPT
                     LEEVVMGYVSAGRRAME"
     gene            1845..2576
                     /locus_tag="VFI12_09350"
     CDS             1845..2576
                     /locus_tag="VFI12_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056655.1"
                     /translation="MAVLGLLPFVVGIVIGVPLVAAEIETGTAVLVWSLDASRRRWLL
                     TRVVIFGTVLTVLLMIPAAAGNLLERFRVPRWEPDTTIYLDFGSRGWLFVERGLVTFA
                     IAVTAGLLIGRVLQGLVVAGLAASVLLVVLNAAHGVGLPAPAPTQVQPDRYVLPVVGA
                     EPRYVDANGRVYSPEQIRAAAPAAVGTPEFFAWFDREGFLQVQFGIPGERLWIVEARE
                     FAVGSAGAIMVLAGATWLAGRRRPY"
     gene            2551..3510
                     /locus_tag="VFI12_09355"
     CDS             2551..3510
                     /locus_tag="VFI12_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056656.1"
                     /translation="MPAGDGRTDVFTLPSLRTSGRVAWHLDALPYAVAAAAALVATAA
                     AMVFAVRLGDSAATDSSDVANLLQATGILGYLIGILPFLIGSILGAPLVARELERRTA
                     GLAWSLSADRRRWLVWRTMPAAAFMVIVLVVLALAGDVLTTARFPGQDPQHSFNDFGE
                     RGPLVVSRGVAAFAVALAAGVRLRRTLPAVLLAVAFSFGLAFASDVTRTYGIAPVELQ
                     DESECAEFSCLNYGTSFRDQNGTIVTFQDALKSAGAAGVTGGPTSDAVREWLDQNGYV
                     EITVAITGQRYPEVEERELAATSILSAAVVLGSVVAVQRARAT"
BASE COUNT          601 a         1157 c         1185 g          767 t
ORIGIN      
        1 gaccatgcgg tccagtccgc gctcgacgcg atccaggtcc acggcgccaa cggttattcg
       61 aacgagttcc cggtcgagcg ctacctgcgc aactcgaagg ccgcggtcat ctacgaggga
      121 acgagccagc tccacacgct cgtccaggcc gactacgccc tcggctaccg cgaggaccgg
      181 cccctgcgct gcgagccctg gccggccgac ggttcgacaa cgtgatctcc tgaccggggc
      241 gcccggatcc tctcccgctt cgaccccgca caacttgcag ccccgaaatt cgctctcacc
      301 gtcgcgggcc gtcgccctgt tccgacccca agcatcggac cttgggcttg tctactagat
      361 cggacagcta ttgatccatt tccaggagct gatgttgtag ttccaactgc cgaggtcaga
      421 ccggctcgta ttccgacaga tcttcaagga tggcccacca aaattgatgt cccagtagaa
      481 gatgatcccc tggccacccg acgcgtgact cagttcgagg ctcgatccaa gaagggagct
      541 tcatcggcta agttgtttcc acggggcatg aagttgttcc ggtagattgg gaagggcgtg
      601 cgggcgtcgc gcggtaggcc ttgcaatcgt cgggcgactg gactagatat tcagcaccga
      661 cgcgtggtcg gcacgtgcgc acgctgcgag atcgtcggca tcgtctcaag ttgtgggacg
      721 cgtttccgta tgcaaagtca catgagcacg ccacgcgaag cgggtgggca gcctgacttg
      781 gaagcgcgcg acgtatccaa gcgatacggc cacggcgtgg tcgcccttga gcacctcgat
      841 atcgctattg ccccatcgac gacgacagcc ctcgtcggac cgaacggcgc cggtaagtcg
      901 accttgatga agttgtggat ggggttcgaa cggcccacga gtggaagcct cagcgtccgg
      961 ggatgggatc cctggcggca acgcgcccac gccgtcgcgg ttctcgggta tgtccctcaa
     1021 tcagtctccc tctacaggga cctgacaatt ggcgaccacc tcgacctggc actgagcctc
     1081 cggccgacat tcgaccgtcc gtacgcgacc gcgcgcctcg cacggctcgg gatcactacg
     1141 cagcgagacg cgggcgagct ctccggcggt gagcagagcc aggtcgcgct tgctctcgct
     1201 ctgggaacgc gggcgccggt gctcttgctc gacgaacctc tcgcgagcct cgatccactg
     1261 gcccgacggg agttcctcgc ggtcgttgga gacgtcgccc gcacggaagg ggtcacggtc
     1321 gtgctctcct cacacatcgt gaccgacgtg gaatcgagct gtgagcggat agtggtcctc
     1381 ggcgcgggcc gagtccttgt gaatgacagc gtcgcgatgc tgctcgctag ccacttgatc
     1441 cgcgtagaca atggcgtggc tggacctcat gtggcgactt gcatcggcgg ctttccgggt
     1501 ccggcgggag aggcgctcac tctttggcaa acatccgggc ccgccgtgag cggatacagg
     1561 cgaccgacac ttgaagaggt cgtcatgggc tatgtgtctg ccggtcggcg agctatggaa
     1621 tgactcgccg cgaacccttt ctagcgctca aaccgcatcg cttctcgctc atctggacag
     1681 cggccggttg ccttctcatc gcgggagccg cggcgctcgt tgctgcttgg cttgcttcat
     1741 acgctgcgcc ggccgcttgt ctggacccgt cattgcggta tacccccgga tgttcgggct
     1801 atcccgaggc cctcgcaatc gatcacgaat gggccgagcg cgtgatggcg gtgcttggcc
     1861 tgcttccctt cgtcgtgggc atcgtcatcg gcgttccact ggttgccgca gagattgaga
     1921 ccggaaccgc ggtgctcgtg tggtcgcttg atgcatcgcg acggaggtgg ttgctcacac
     1981 gggtagtcat cttcgggacc gtcctgaccg tgttgctcat gattcccgcg gcagctggga
     2041 atctgctgga gcgattccgc gttccacgat gggagccaga taccacgatc taccttgact
     2101 tcgggagtcg tggatggctc ttcgttgagc gcgggctcgt cacttttgcg atcgcagtta
     2161 cggctggtct gctcatcggc agggttctac agggcttggt agttgccgga ctcgccgcgt
     2221 ctgtattgct cgtagtcctc aacgccgccc acggcgtggg tctgccagcg cccgcgccaa
     2281 cgcaagtcca acctgatcga tatgtgcttc ccgttgtcgg cgccgagccc aggtacgttg
     2341 acgcgaatgg gagggtgtat tcccccgaac agatacgtgc cgccgctccg gctgccgtcg
     2401 gcacaccaga gttctttgcc tggttcgatc gggagggttt tcttcaggtg caatttggaa
     2461 tccccggcga gcggctctgg atcgtggagg cacgcgaatt cgctgtcggg tcggcgggtg
     2521 ccatcatggt cttggccgga gcgacatggc ttgccggcag gcgacggccg tactgacgtg
     2581 ttcacgctgc cttcgttgcg tacgagcggg cgggttgcat ggcatctgga cgcactcccg
     2641 tacgcagttg cggctgcggc cgccctcgtc gccaccgcgg ctgcgatggt gttcgctgtg
     2701 aggcttgggg attcggccgc cacggacagc tccgatgtgg cgaacctgtt gcaggcgacg
     2761 gggatcttgg ggtacctgat cggaatcctt ccgtttctga tcggatccat ccttggtgcg
     2821 cctcttgttg ctcgggaact tgaacgccgg accgcgggac tagcatggtc gctgagcgct
     2881 gatcgtcgtc gttggctcgt ttggcggacg atgccagccg ctgccttcat ggtcatcgtc
     2941 ctcgttgtgc tcgctctcgc tggcgacgtc ctcacaacag cccgattccc cggacaggac
     3001 ccgcagcatt cgttcaacga ctttggcgag cgaggaccgc tcgtcgtgag tcgcggggtt
     3061 gcggcgttcg ctgtcgccct tgcggcgggc gtccgtctcc ggcggacgct gcccgccgta
     3121 ctgcttgccg tcgcattttc cttcggcttg gcgttcgcgt cggacgtgac ccggacatac
     3181 ggaattgccc cggtggaact tcaggacgag agcgaatgcg ccgagttctc ttgcctcaac
     3241 tacggaacgt cgttccgcga ccagaacggc accattgtca catttcagga cgcgttgaaa
     3301 tcggccggtg cggcgggagt gaccggtggg ccaacgtcgg atgcagtgcg agagtggctc
     3361 gaccagaatg gttatgtcga gatcacggtt gctatcacgg gtcaacgcta tcctgaagtc
     3421 gaagaacgtg agttggctgc gacgtcaata ttgtccgccg ccgtcgttct tggttccgtt
     3481 gtcgcggtgc aacgcgcccg ggccacctga aggacccgaa caaagcggca tgcataacgg
     3541 ttggagactt gtcactcaat cccttctatc gatcccgccg gacctcgagc cgcgacgtgc
     3601 atcgcgcgcg cgggagctcg gcggacggat gcaaggagat gcaaagtgag cgtcgatctg
     3661 gacttcgacc cccatcacac gagtaatcgc gaggtcgata tcttgcaggt
//
LOCUS       DASSGX010000519         3003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_965970, whole genome shotgun sequence.
ACCESSION   DASSGX010000519 DASSGX010000000
VERSION     DASSGX010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_965970"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..2501)
                     /locus_tag="VFI12_09360"
     CDS             complement(120..2501)
                     /locus_tag="VFI12_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET7056657.1"
                     /translation="MAATVDAPERMFGKAIKRREDPRLITGAAAFLDDIKLAGMSHAA
                     LLRSPHGHARIRSIDTSAAVAAPGVIGVFTGQDFADLNPLPCAWQAGGVQNNVNTPRV
                     LAIDEVHHVGDPIAVVVAEDVKSATDAVDLINVDYEVLPAVVDARKAAEAGAPQLHDN
                     APNNVVMHWMCGKGEDEVRTAIDAAEIKVSQRIINQRLIPTAMETRGSIGRFDPGAGE
                     YTLWATSQAPHVMRLLITAFVMGIPEQKLRVISPDMGGGFGSKIFLYFDMPLVLALAR
                     KVGRPVKYVEDRSENYMSTTHGRDHITDLEIGATRDGKIVGMNVKTYANMGGYLSTIA
                     PGIPTTLYGRMLSGCYKVPAIHCDVFGVYTNTSMVDAYRGAGRPEAAFVIERTCDLVA
                     DATGVDPAEVRRKNFIQPEDFPYDNGVGMLPYDSGNYEPALDKALEAVGYRQLREEQQ
                     RRINEGADKLLGIGLSSYVEVCGVAPSKWIGLQGEGWGAGLWESANIKVHLTGKVVVT
                     TGSQPHGQGHETTFAQLVSDQLGIPYDDIEVEHSDTLGAPFGYGTYGSRSLSVGGTAI
                     YKSTAKIMDKARRITAHLLEANFEDVIYEDGKCFVKGSPETAKTIQEIALAASVGYDL
                     PREIEPFLDETTYYDPPNCTFPFGTHICVVEIDRETGQAAPIKYIAVDDVGNVLNPLI
                     VDGQVHGGIAQGLAQALYEGAYYDEDGQLLTGTMSDYAVPKASMVPSYEMDKTCTPTT
                     VNPMGVKGAGEAGTIAATPAVANAVIDGLKHLGVKHIDMPMTPERVWRAMNPS"
     gene            complement(2568..>3003)
                     /locus_tag="VFI12_09365"
     CDS             complement(2568..>3003)
                     /locus_tag="VFI12_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET7056658.1"
                     /translation="AIFVDDGTNLLVALRELIGDMTPKFGCGQGGCGACTVLVDGEPV
                     LSCITLAESVAGKSIETADGLKNGPELHPLQTAFMEAFAAQCGYCTPGMIMAGVSLLK
                     RNPNPSEEEIRHALEGNICRCTGYQNIVKSIQWAAEKMQQNA"
BASE COUNT          503 a          972 c          941 g          587 t
ORIGIN      
        1 agggtcgccg gccaggagcg aaatcgcctc ctgcacagaa tttggccgct gatattcgaa
       61 ctgtcctgga tacacgatga ctccttccgg ggaccaccca actgatacgc ggattctacc
      121 tatgacgggt tcattgcgcg ccagacccgc tctggcgtca tcggcatgtc gatgtgcttg
      181 acgccgagat gcttcaaacc gtcgattact gcattggcga cggcaggcgt cgccgcgatc
      241 gtaccggcct cgcccgcacc cttgacgccc atcgggttga ccgttgtcgg cgtgcaggtc
      301 ttgtccatct cgtagcttgg gaccatgctc gccttcggca ccgcatagtc gctcatggtg
      361 ccggtcagga gctgtccatc ctcgtcatag tacgcgccct cgtagagcgc ctgagccaac
      421 ccctgcgcaa tgccgccatg cacctggcca tcaacgatca gcgggttgag cacgttgccg
      481 acgtcatcca cggcgatgta cttgatcggc gccgcctgcc cagtctcccg gtcgatctcg
      541 accacgcaga tgtgcgtccc gaatgggaag gtgcagttcg gcggatcata gtacgtcgtc
      601 tcgtcgagga atggctcgat ctcgcgcggc aggtcgtacc cgacgctggc cgcgagagcg
      661 atctcctgga tcgtcttcgc cgtttcgggc gagcccttca cgaaacactt gccgtcctca
      721 tagatgacgt cctcgaagtt cgcctcgagc aggtgcgccg tgattcgccg cgccttgtcc
      781 atgatcttgg cagtgctctt gtagatggcg gtcccgccga ccgaaagcga gcggctgccg
      841 taggtgccgt atccgaacgg ggcgccgagc gtgtccgaat gctcgacctc aatgtcgtcg
      901 tacgggatgc cgagctgatc ggaaacgagc tgcgcaaagg tcgtctcgtg gccctgacca
      961 tgcggctgcg acccagtcgt gacgacgacc ttgccggtca ggtggacttt gatgttggcg
     1021 ctctcccaga gacccgcgcc ccatccttcg ccttgcaacc cgatccactt ggatggcgcc
     1081 acgccacaga cctcgacgta ggacgagagc ccgatcccga gcagcttgtc cgcaccctcg
     1141 ttgatgcggc gttgctgctc ttcgcgaagc tggcggtatc cgaccgcctc gagcgccttg
     1201 tccagtgccg gctcgtagtt gccactatcg tacggcagca tgccgacgcc gttgtcgtac
     1261 gggaaatcct ccggctggat gaagttcttg cgccggacct ccgccggatc gacaccagtc
     1321 gcatcagcca cgaggtcgca tgtccgctcg atcacgaacg ccgcttccgg ccggccggcg
     1381 ccgcgatagg catcgaccat gctggtgttg gtgtagacgc cgaacacgtc gcagtggata
     1441 gccggcacct tgtagcagcc ggagagcatc cggccgtaga gggtggtcgg gatgcccggc
     1501 gcgatcgtcg agaggtagcc acccatgttg gcgtacgtct tgacgttcat gccgacgatc
     1561 ttgccgtcgc gggtcgcgcc gatctcgagg tcagtgatgt gatcacgacc gtgcgtggtg
     1621 gacatgtagt tctcggagcg atcttcgacg tacttgaccg ggcggccgac cttgcgggcc
     1681 agtgccagca cgagcggcat gtcgaagtag aggaagatct tgctgccgaa gccgccaccc
     1741 atgtcgggcg agatgacgcg cagcttctgc tccgggatgc ccatcacgaa ggccgtgatc
     1801 aggagccgca tgacgtgcgg cgcctgtgac gttgcccaga gcgtgtactc gcccgcgccc
     1861 ggatcgaacc ggccgatcga gccgcgcgtc tccatcgcgg tcgggatcag ccgctggttg
     1921 ataatccgct gggagacctt gatctccgca gcgtcgatcg ccgttcggac ctcgtcctca
     1981 cccttcccgc acatccagtg catgacgacg ttgttcggcg cgttgtcatg gagttgcggc
     2041 gcaccggctt cggcagcctt gcgggcgtcg accacggccg gcaggacctc ataatcgacg
     2101 ttgataaggt cgaccgcgtc agtcgctgac ttgacatctt ccgcgactac gacggcaatc
     2161 ggatcgccga cgtggtgcac ttcgtcgatg gccaaaacgc gtggcgtgtt gacgttgttc
     2221 tggacgccgc ccgcctgcca ggcgcagggc aatggattga gatccgcgaa gtcctgcccg
     2281 gtgaagacac cgatcacgcc cggcgctgcc acggcagccg aagtgtcaat agatcgaatc
     2341 cgggcgtgac cgtgggggga ccggagcaac gcggcgtggc tcatcccggc caacttgatg
     2401 tcgtccaaga atgcggccgc gccggtgatc aggcgggggt cttcccgccg cttgattgcc
     2461 ttgccgaaca tgcgctcagg cgcatcgact gtcgctgcca tgtcgttatc ccatcactgg
     2521 aacggtccgc gccgccccat cgcggcgcgg tttcacaacg attggattta cgcgttctgc
     2581 tgcatcttct cggccgccca ctggatggac ttgacgatgt tctggtaccc ggtgcagcgg
     2641 cagatattgc cttccagcgc gtgccggatc tcctcttcgc tcgggttcgg gttgcgcttg
     2701 aggagcgaca caccagccat gatcatgcca ggcgtgcaat agccgcactg cgcggcgaag
     2761 gcctccatga acgccgtctg caagggatga agctccggac cattcttgag gccgtccgcg
     2821 gtctcgatcg attttccggc cacgctctca gcgagggtga tgcaggagag gacgggctcg
     2881 ccgtcgacca gcacagtgca ggcgccgcag ccgccctggc cgcagccgaa tttgggcgtc
     2941 atgtcgccga tcaactcgcg caaggcgacg agcaggttgg tgccgtcatc gacgaagatc
     3001 gcg
//
LOCUS       DASSGX010000520         2560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_309278, whole genome shotgun sequence.
ACCESSION   DASSGX010000520 DASSGX010000000
VERSION     DASSGX010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309278"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..1582
                     /locus_tag="VFI12_09370"
     CDS             146..1582
                     /locus_tag="VFI12_09370"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HET7056659.1"
                     /translation="MDWSDAVFLARVQFAFTVSFHFLFPALTIGLASYLAVLQGLYLW
                     TGREVFHSLFDYWKRIFAVAFGMGVVSGIVMTYQFGTNWSVFSERAGPVLGPLMAYEV
                     FTAFFLEAGFLGIMLFGLNRVGRGLHMFATVMVAFGTAFSAFWILSVNSWMHTPAGYA
                     INEAGQFIPEDWWAAIFNPSFPYRLVHTLFAAYLTTAFAIGGVAAYHLLKDRSNEGSR
                     IMFSMAMWMAAIVAPLQIIAGDLHGLNTFEHQPAKIAAMEGHFETEAGAPLILVGWPD
                     MEAETTRYALQIPNLGGLILTHEWDGVVRGLKEWPKQDWPHVPTVFWSFRIMVLIGLL
                     MATLGIWATWMRVRGRLYDSDWLLRAAVAMGPSGFVAVIAGWITTEVGRQPYTVYGLL
                     RTVDSASPIGAAAVGSSLVAFFVVYMLVFGAGTFYILRLSGALPKAVEPEMPHRPVRA
                     AGITPAPALAGGGADTTDREGEEDERDR"
     gene            1572..>2560
                     /gene="cydB"
                     /locus_tag="VFI12_09375"
     CDS             1572..>2560
                     /gene="cydB"
                     /locus_tag="VFI12_09375"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HET7056660.1"
                     /translation="MTVDLAFVFAAVIAIAVLLYVIFDGFDLGIGILFPFFPEEADRR
                     RMMNSIAPVWDGNETWLVAGGGGLLAGFPLAYGIIFSALYAPILAMVMALIFRGVAFE
                     FRFRSRRRRHLWDLGFAVGSIVAAFAQGVTLGALIQGIAVANRAYAGGWWDWLTPFSV
                     LTGVALVIGYALLGATWLIKKTDGELQDRAYDFAFRSGVGTLVAIGIVSLATPFLHPV
                     YMERWFSWPAVLYTLPVPLLVILMAYILYQSLVQRWTYIPFASSIGLFVLAYVGLGIS
                     IFPLVVPPGITIWDAAAPDSSLAFVLVGAAILLPVILAYTAYSYWVFRGKVSS"
BASE COUNT          352 a          819 c          821 g          568 t
ORIGIN      
        1 ctgcggccgc gaacgacgtc cgggcctgac gtgcactgtc tgcgcccagg cttctggccg
       61 ggaacgtaca tcgctctttc gtgttacgtc tgagcgcgtg tgccgctgag ccgcgcgcgg
      121 ctcgtggcgt gccggagcgg atgcgatgga ttggagcgac gcggtattcc tggcgcgagt
      181 ccagttcgcg ttcacagtca gctttcactt cctcttccct gcactgacca tcggtcttgc
      241 cagctacctg gccgtcttgc agggcctgta cctgtggacg ggacgggagg tctttcactc
      301 gctcttcgac tactggaaga ggatcttcgc ggtcgccttc ggaatgggcg tcgtctccgg
      361 catcgtgatg acgtaccagt tcggcacaaa ctggagcgtg ttctctgaac gggccggtcc
      421 ggtgctcggg ccgctcatgg cctatgaggt cttcaccgcc ttcttcctgg aggccggctt
      481 ccttggcatc atgctgttcg gcctcaaccg ggtcggccgg ggcctgcaca tgtttgccac
      541 tgtcatggtg gcattcggaa cggcgttctc cgccttctgg attttgtccg taaacagctg
      601 gatgcatacg ccggccggct atgcgatcaa cgaggctggc cagttcatcc cagaggactg
      661 gtgggcggcg atcttcaatc cgtccttccc gtatcgcctc gtccacacgt tgtttgccgc
      721 ctatctgacg acagccttcg ccatcggcgg cgtcgccgcc tatcacctgc tgaaggaccg
      781 gagcaatgaa ggctcacgga tcatgttctc gatggccatg tggatggcgg ccatcgtcgc
      841 acctctgcag atcatcgccg gcgacctgca tggcctgaat acgttcgagc atcagcctgc
      901 gaaaatcgcg gccatggagg gccatttcga gaccgaggcc ggcgcgccgc tcatcctggt
      961 gggctggccc gatatggagg ccgagacgac ccgctatgcc ctccagattc cgaacctcgg
     1021 cgggctgatc ctgacgcacg agtgggacgg tgtcgtcaga ggcttgaagg aatggccgaa
     1081 gcaggactgg ccccatgtgc cgaccgtctt ctggtcgttc cgcatcatgg tgctgatcgg
     1141 tctgctcatg gccacgctgg ggatctgggc cacttggatg cgcgtccgcg ggcggctcta
     1201 cgactcggac tggctgcttc gggcggcggt ggccatgggg ccgtccggct tcgtcgccgt
     1261 catcgcgggc tggatcacca ccgaggtcgg gcgccagccc tacaccgtat acggattgct
     1321 tcggactgtg gattctgcct ctccgatcgg cgctgccgcc gtgggcagct cacttgtcgc
     1381 gttctttgtc gtgtacatgc tcgtgttcgg tgcgggcact ttctacatcc tgcgcctctc
     1441 gggggctctg ccaaaggcgg tcgagccaga gatgccccat aggccggtac gcgccgccgg
     1501 cataacccct gcgccggcgc tggccggtgg cggggcggac actaccgatc gagaagggga
     1561 ggaagacgaa cgtgaccgtt gaccttgcct tcgtgtttgc ggctgtcatc gcgatcgcgg
     1621 tgctgctcta cgtgattttc gacggtttcg acctcggcat cggcatcctg ttccccttct
     1681 ttcctgaaga agccgacagg cggcggatga tgaactcgat cgcgccggtc tgggacggca
     1741 acgagacctg gctggtggcg ggcggcggcg gcctgctggc ggggttcccg ctggcctacg
     1801 ggatcatctt ctccgctctt tacgcgccga tcctcgccat ggtcatggcg ctcatcttcc
     1861 gcggtgtcgc gttcgagttc agattccggt cccggcgccg caggcatcta tgggacctcg
     1921 gattcgcggt cggttcgatc gttgccgctt tcgcgcaggg cgtgacgctg ggggcactca
     1981 tccagggcat cgccgttgcg aaccgcgcct atgccggcgg ctggtgggac tggctgacgc
     2041 cgttcagcgt tctgaccggc gtcgctctcg ttatcggcta cgccctgctc ggcgcgacgt
     2101 ggctcatcaa gaagaccgac ggagagcttc aggacagggc ctacgacttc gccttccgct
     2161 ccggcgtcgg cacgctcgtg gcgatcggga tagtgagcct tgcgacgcca tttctccacc
     2221 cggtctacat ggagcggtgg ttcagctggc cggcggttct ctacaccttg ccggtgccgc
     2281 tcctcgtcat cctgatggcc tacatcctct accagtcgct ggtgcagagg tggacgtaca
     2341 ttcccttcgc ctcctcgatc ggactgttcg tgttggccta cgtgggtctc gggatcagca
     2401 tctttccgct cgtcgtgccg ccaggcatta cgatctggga tgcggcggct ccggacagca
     2461 gccttgcttt cgtgctggtc ggagcggcga tcctgctgcc ggtcatcctt gcctacaccg
     2521 cctattccta ctgggtcttc cgcggcaagg tcagctccga
//
LOCUS       DASSGX010000521         2561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1619429, whole genome shotgun sequence.
ACCESSION   DASSGX010000521 DASSGX010000000
VERSION     DASSGX010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1619429"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VFI12_09380"
     CDS             <1..455
                     /locus_tag="VFI12_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056661.1"
                     /translation="QGRLPDWLQMISERPPYIDGTNDWTDRTVWINRLIRDGWTRREG
                     AVPETWERRSPSGDMTLAMRIVTNIDFRAYGGRYVIEYAVGTNPDGQEVELGRATWAD
                     WDQRGRLIVARDGRLMHWQPSGFTDIADFNDQTPDPQPAPAHVTDWPT"
     gene            complement(685..1752)
                     /locus_tag="VFI12_09385"
     CDS             complement(685..1752)
                     /locus_tag="VFI12_09385"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HET7056662.1"
                     /translation="MLAIDAHLDLAMNAVLWDRDLKLSAHETRQIERSEGMTEKGRCA
                     GTVGIPDLRAGEFGLVFATVLARTNQGLNSGIDFRTQDNSYAQAQGQLALYREFERQG
                     IMRMIRTAADLTQWKSEWDANPATTPIGYVLLMEGGDPIVEPEQARGWFDDGLRMVGL
                     AHYGPSQYAYGTASAGPITPRGKELLKVMDEVGMIQDMSHLTDQSFYEALDLFQGPIF
                     ASHSNCRALVPGDRQISDDMIKRMVERDAVIGAVFDAWMLQPNWERGVTTNENVTIAT
                     VVDHIDHVCQIAGNADHAAIGTDLDGGYGIEQTPKDLDTIVDVQNVADILRNRGYAEA
                     DVAKIMHGNWLRFLERALPAN"
     gene            2241..>2561
                     /locus_tag="VFI12_09390"
     CDS             2241..>2561
                     /locus_tag="VFI12_09390"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7056663.1"
                     /translation="MAEELKDEEFRALWEATAPARALANRLLEYRIEHGLSQTKLARK
                     LGLKQPAVARLEAGEHMPTIKTLLRIAEALDIEILLDIKPSTRQESFVSSRADKARVV
                     ERVTT"
BASE COUNT          443 a          772 c          899 g          447 t
ORIGIN      
        1 atcagggccg gctaccggac tggctgcaaa tgatcagcga gcggccgccg tacatcgacg
       61 ggacaaacga ctggaccgat cgcaccgtct ggataaaccg gctgatccgt gatggctgga
      121 cccggcggga gggtgcggtt ccggaaacgt gggagcgacg gtcaccttcg ggtgacatga
      181 cgctggcgat gcggatcgtg accaacatcg atttccgggc gtacggcggg cggtacgtga
      241 tcgagtatgc cgtcggaacg aatccggacg gtcaggaggt cgaacttggc cgggcgacct
      301 gggccgactg ggaccagcgc ggccggttga tcgtcgcccg ggacggccgt ctgatgcatt
      361 ggcaaccgtc gggattcacc gacatcgctg acttcaacga ccagacgccg gacccgcaac
      421 ccgcgcccgc gcacgtcacc gattggccga cgtaggggat tgcggcagca aacccgcgcc
      481 tccccattgc cggtttccac gcccagagcc ggttggaggg gtccgcgggg gcggacccgg
      541 ggcgccgcac aagggtccgg tggcaggcaa caggccggtt ggcgaggcgc ggccgtgccc
      601 ccgcacggcc ctacaggcgt gccagcccac ctcaaccctc cacgcggggc gggtttgcgg
      661 cagcaacccc tacaggagtg gtggttagtt tgcggggagg gctcgctcca ggaatctcag
      721 ccagttgccg tgcatgatct tggcgacgtc ggcctcggcg tatccgcgat tgcgcaggat
      781 atcggccacg ttctgaacgt cgacgatcgt gtcgagatct ttcggtgttt gctcgatacc
      841 gtagccgccg tcgagatcgg tcccgatcgc ggcgtggtcg gcgtttccgg cgatctggca
      901 gacgtggtcg atgtggtcga cgacggtcgc gatcgtcacg ttctcgttcg tcgtcacgcc
      961 gcgctcccag ttcggctgga gcatccaggc atcgaagacc gcgccgatca ccgcgtcccg
     1021 ctcgaccatc cgcttgatca tgtcgtcgct gatctggcgg tcgccgggga cgagggcgcg
     1081 gcagttcgag tggctggcga agatcggccc ctggaacagg tcgagcgcct catagaagct
     1141 ctggtcggtc aggtgcgaca tatcctggat catcccgacc tcgtccatca ccttgagcag
     1201 ctccttgccg cggggcgtga tggggccggc gctggcggtg ccgtaggcgt actggctggg
     1261 gccgtaatgg gcgaggccga ccatccgcag gccgtcgtca aaccagccgc gggcctgctc
     1321 cggctcgacg atcgggtcgc cgccttccat cagcaggacg tagccgatcg gcgtcgtggc
     1381 cgggttggcg tcccactccg atttccactg tgtgaggtcg gcggcggtgc ggatcatgcg
     1441 catgatgccc tgccgttcga actcgcggta gagggcgagc tggccctggg cctgggcgta
     1501 ggagttgtcc tgcgtgcgga aatcgatccc gctgttcagc ccctggttgg tgcgggcgag
     1561 aaccgtcgcg aagacgagcc cgaactcgcc ggcgcggaga tcggggatgc cgacggtgcc
     1621 ggcgcagcgg cccttctcgg tcatcccctc ggaccgctcg atctggcgag tctcgtgcgc
     1681 gctgagcttg agatcgcggt cccagaggac ggcattcatg gccaggtcaa ggtgggcatc
     1741 aatcgcgagc acggttcctc ctgagacgtg agttgcggtc agtcgattgc gatagggtat
     1801 agggtatcgc gaatccggtc cggatggacc gggcgttgct gtgataacat tcttggtata
     1861 aggcgggtgc atggaggtca ttttctgcga cgaggcggag aacgaacggc gagcgatgcc
     1921 catgagagaa cgccaggcca tgatcgccgc agtcgaaaag ctcgaacagg ttggcgatcg
     1981 cttgggatcg ccgcacagca gtgcagtgaa gggcgccgag gcaacactgc gggagcttcg
     2041 accacgaggc ggccgcagcc cttggcgagc gttctaccga cggatcggag acgtcatggt
     2101 agtcggcgcg atcgggcccg aggccggagt tgatcgacgc ggctttaatc gcgcggtaga
     2161 tgcggccgtc gtgcggctcg ccggatttga agagaggtat aggaatgcct aagtacgggc
     2221 tccggacggc agcggagata ttggccgagg aattgaagga cgaggagttt cgagctttgt
     2281 gggaggcgac tgcccctgcc cgggcgcttg caaatcggct cctcgaatac cgcattgagc
     2341 acgggttgtc ccagacgaaa ctggcgcgca agctcgggct caagcagccg gccgtagccc
     2401 gcctcgaagc gggcgagcat atgccgacga tcaagacact tctccggatc gccgaagcgt
     2461 tggacatcga gattctcctc gatatcaaac catcaaccag gcaggagtca tttgtctcgt
     2521 cgcgagccga caaggcacgc gtcgtcgagc gcgtaacgac c
//
LOCUS       DASSGX010000522         2367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_966812, whole genome shotgun sequence.
ACCESSION   DASSGX010000522 DASSGX010000000
VERSION     DASSGX010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_966812"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VFI12_09395"
     CDS             complement(<1..423)
                     /locus_tag="VFI12_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056664.1"
                     /translation="MQDPNRNRLPRNPFTGGGDKNDKNEKGGNSRRPRVPTWFVGLLL
                     VCIAVWYAYQFMSPSSNQDEVTVPYSVAREQIATGNATTAVIGTSNLEIDLKSPIHWD
                     GDNDTVVPKDAANASDFRTVSKLKANIPPPIRQDNSELI"
     gene            complement(621..971)
                     /locus_tag="VFI12_09400"
     CDS             complement(621..971)
                     /locus_tag="VFI12_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056665.1"
                     /translation="MRDSLAHGDIIQPDRVDELMDFLAPFVHMLLTQLEPGEFTTPMF
                     IEFMRSIPEGEALYQEALRRWGEANEHMSKMVIHGQVIPGILRRSPTVEWAGFAHGEP
                     DPFGVPAWWRLRES"
     gene            1066..2313
                     /locus_tag="VFI12_09405"
     CDS             1066..2313
                     /locus_tag="VFI12_09405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET7056666.1"
                     /translation="MTTATNGQKGSMSGGDLPETADIVVIGAGVNGLATAFELTRRGA
                     GKVVVLERRHVGAGATGKSGALVRQHYTNVPEALLTLHSLDTFRNWGDVVGHGDPRFV
                     QPGFLRVVAPDDETRIRANVENLKAAGINTWIVTPDEIAEIEPLLFTGDISVAAFEPD
                     AGYADPNATAYAYAEAAVANGATLVLDCEATGIDIQGDRVVAVETNRGRIETRQVLVA
                     AGPWSDRLLDPLGYSIGLMPYKSQVVIFRWPMAMDQSRTHRVVIDTIQESWLRPEGDA
                     GTLIGIEFPDRKGDPDTFPEFPPINYIEQCRSALAGRFPVFANATMRGGWTGMYMMSP
                     DHHPIIGTLDKAEGLFVMTGDSGSCFKTAPATGVCLAELMTEGEAKLVDLTPFRPSRF
                     AEGKPWVDEHQYGEVGNELSISR"
BASE COUNT          436 a          743 c          754 g          434 t
ORIGIN      
        1 gatgagctcg ctgttgtcct gccggatcgg gggcggaatg ttggccttca gcttcgacac
       61 cgtgcggaag tccgaggcat tggccgcgtc cttcggtaca accgtgtcgt tgtccccatc
      121 ccagtggatc ggcgacttga gatcgatctc gaggttgctc gtgccgatca ccgccgtcgt
      181 ggcattgccg gtggcgatct gctcccgggc gacggaatac ggaaccgtga cctcgtcctg
      241 gttcgagctc gggctcatga actggtaggc ataccagacg gcgatacaaa cgagcagcag
      301 gccgacgaac caggtcggca cgcgcggccg gcgggaattg ccgcccttct cgttcttgtc
      361 gttcttgtcc ccgcctccgg taaacggatt gcgcgggaga cggtttcggt ttggatcctg
      421 cattaattgt ccttacggtc gagcatggag accgtttcca aaagattggc caccggtgga
      481 ccggggcctc aggtgcgtat cggctgatcc aaagggagtc gaccgttcgg aaaatcacgc
      541 aatatgttga gctaatccta ccgcccctga gcggcagatt tccattatcg ccggtccggc
      601 aatctccgtc ccgtgacccg tcaagactcc cgcaatcgcc accaggccgg caccccgaat
      661 ggatccggct cgccatgtgc gaaccccgcc cattcaacgg ttggcgaacg ccggaggatg
      721 cccggaatca cctgtccatg gatcaccatc ttgctcatgt gctcgttggc ctcgccccat
      781 cggcggagcg cctcctggta caacgcttcg ccctcgggga tcgagcgcat gaattcgatg
      841 aacatcggcg tggtgaactc gcccggctcg agctgggtca aaagcatatg tacgaagggc
      901 gccaggaaat ccatcagctc atcgacccgg tcaggctgaa tgatgtcacc gtgggccagg
      961 gaatcgcgca tgaagatcga cctcgatgcg ggtcacgaat atcgccggca gtgccgcata
     1021 cttccgtagc ggattagcga cagtgccaat gggagcgtat cagcaatgac gacggcgacg
     1081 aatggtcaga agggatcaat gtccgggggc gaccttcctg aaactgcgga cattgtcgtc
     1141 atcggcgcag gtgtgaacgg gttggccacc gcattcgagc tgacccggcg cggcgcgggc
     1201 aaggtcgtcg tcctcgagcg gcggcacgtc ggagccgggg cgactggcaa gtccggcgcg
     1261 ctggttcggc agcactacac gaacgtcccc gaggcgctcc tgaccctcca tagcctcgac
     1321 accttccgga actggggcga cgtcgtcggc cacggcgatc cgcgcttcgt ccaacccggc
     1381 ttcctgcgcg tcgtcgcgcc ggacgacgag accaggatcc gcgccaacgt cgaaaacttg
     1441 aaggcggccg gcatcaatac ctggatcgtg acgccggatg agatcgccga gatcgaaccg
     1501 ctgctcttca ccggcgatat ttccgtcgcg gcattcgagc cggatgccgg gtacgccgat
     1561 cccaacgcga ccgcatacgc ctatgcggaa gcggcggtcg cgaacggcgc gacgctggta
     1621 ctcgattgcg aagcgaccgg gatcgacatc cagggtgacc gggtcgtcgc agtcgagacg
     1681 aaccggggcc gcatcgagac gcggcaggtg ctggtcgcgg ctggaccatg gtcggatcgg
     1741 ttgcttgatc cgctcggcta ttccatcggt ctgatgccct acaagtcgca agtcgttatc
     1801 tttcgctggc cgatggcgat ggaccagagc cgcacgcacc gggtggtcat cgacactatc
     1861 caggagtcgt ggctgcggcc ggagggcgac gcgggcacgc tgatcggcat cgagttccca
     1921 gaccgcaagg gcgaccccga tacgttcccg gagtttcctc cgatcaacta catcgagcag
     1981 tgccgctccg cgctggccgg ccgcttcccg gtctttgcga acgcgaccat gcgcgggggc
     2041 tggaccggca tgtacatgat gagcccggat caccatccaa tcatcggtac gctggataag
     2101 gccgagggtc tcttcgtgat gacgggcgac agtggctcgt gcttcaagac ggctcccgcc
     2161 accggcgtct gcctcgctga gctgatgacc gagggcgagg cgaagctggt cgatctgacg
     2221 ccattccggc cgagccggtt cgccgaaggc aagccctggg tggatgagca tcaatatggc
     2281 gaggtcggaa acgagctcag catctcccga tgagctcggc gcacacgctc tacctgttcc
     2341 actgcgggac tctgatcgag cccaatg
//
LOCUS       DASSGX010000523         4523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1511592, whole genome shotgun sequence.
ACCESSION   DASSGX010000523 DASSGX010000000
VERSION     DASSGX010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4523
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1511592"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="VFI12_09410"
     CDS             <1..620
                     /locus_tag="VFI12_09410"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HET7056667.1"
                     /translation="VQMPGLDGFEVAHRLLERGEDAPGVIFVTAYDQRAIAAFEVNAV
                     DYLLKPVDATRLDQALQRARRRLAADRGAPVPLNDQLERIVKLMADREVRREQVAVKV
                     GERFMLVQAEEIIFASLADESINIVTGQVSGTSNYRTLDDLQARLDPAVFWRVHRSHL
                     VNINKIKEIVPWFSRNYILRMKDMKGTEIPVSRSQTKRLREYLKL"
     gene            716..1297
                     /gene="lepB"
                     /locus_tag="VFI12_09415"
     CDS             716..1297
                     /gene="lepB"
                     /locus_tag="VFI12_09415"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET7056668.1"
                     /translation="MAAAAQMRMDLEDPAQVVLQRLNVWKDELIAWFKTLVSAAVYAT
                     LIVTFGFQVARVEGQSMAPTLSDQDRLIVNKFAYRIGAPRRGDIVMLYYPINPDKSFV
                     KRVIAEEGDTVRIVDGRVYVNDVPMDDSFVPPEFRSHDDYGPEVIKEGYYFVMGDHRN
                     NSSDSRHWGLVPKKYIIGKVQLRWWPLPHAHVF"
     gene            1368..2270
                     /locus_tag="VFI12_09420"
     CDS             1368..2270
                     /locus_tag="VFI12_09420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET7056669.1"
                     /translation="MNDRYGRVATVLWRVMFLNLAVALAKIGLGHYSGTVSIISEGYH
                     SLTDTAGNIVGLIGVSIARRPPDENHPYGHRKYETMASIGIMVFLIVVLVQVLSRSAD
                     RFLHGGTPHVFPEGIALMAITLVINILVVSYESREGRRLKSEVLLADARHTRSDVLTS
                     TAVLGALIGVWAGYPILDPMAALLVAIFIGHACWRIAQEASPILGDEMMMDVHEVQQV
                     VQSVPGVLGSEKIRARGSADYVFLDLHLWLDPQTPLFDAHQTSHVVKDTLMAKFPQLM
                     DVVIHIEPPPSAAADNSQPPTRKS"
     gene            2424..2837
                     /locus_tag="VFI12_09425"
     CDS             2424..2837
                     /locus_tag="VFI12_09425"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HET7056670.1"
                     /translation="MEPQIPQTESLASLVRGVLMDLRTLIREEISLARVEIREQATRA
                     RAAAMSFGIAAGALVFGGIFLLIAVAMGLADLLNWPVWAGFLIVAIVLGLVGAFTLSS
                     GRRQLRAFRAVPEETVTTLKENSEWIARRLSSGQR"
     gene            2801..3067
                     /locus_tag="VFI12_09430"
     CDS             2801..3067
                     /locus_tag="VFI12_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056671.1"
                     /translation="MDRQAAVIRAEMSETRAELDRKISRLEARAREFTPQQLKDRYVP
                     EYFLDRAIGVALTLIGVGLAFGAWQRRRTRRDTVRTIAAAYGCW"
     gene            3162..4514
                     /locus_tag="VFI12_09435"
     CDS             3162..4514
                     /locus_tag="VFI12_09435"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7056672.1"
                     /translation="MNTNDPREIIDQSPMTVAQIVVVILTVCMNGIDGFDVLSIAYSS
                     PGIAKEWGIAQTGLGVVLSMELIGMAIGSVLLGGVADRIGRRPTLLACIIAMVVGMLG
                     ATTAASPLQLSMWRVFTGLGIGGMLSAINAVVAEFSNKRWRSLSISMMVLGYPLVGAT
                     GGMVASALLKTHDWRSVFYLGATGTLVLLPAVYLLMPESIHWLARKQPANALARINAA
                     LAKIGHRSISAVPQVLESERKKSVADIFSPALGVVTVMVTAAYFFHIVTFYFLLKWTP
                     KIVADMGFAPSSAGSILAWANVGGALGTALFGVLTARVGLKRLSIVILLLSAVAIAVF
                     GRTPQELGTMAWLAAIAGFFGNAGVSGLYSIAAYAFPTHVRATGTGFVIGVGRGGAVL
                     APILAGYLLQTGFPLPTVALIMGVGSLLGAVVLMFLKLDDQRPVAERAPQMRTSTARA
                     "
BASE COUNT          768 a         1452 c         1442 g          861 t
ORIGIN      
        1 atgtgcagat gccggggctg gacgggttcg aggtggcgca ccggctgctc gaacgaggcg
       61 aagatgcgcc cggcgtgatc ttcgtcaccg cctacgacca gcgggcaatc gccgccttcg
      121 aggtgaatgc cgtcgattac ctgctgaaac cggtggacgc gacccggctc gaccaggcgc
      181 tccagcgcgc tcgcagacgt ctggccgccg accggggggc gccggtgcca ctaaacgacc
      241 aactcgagcg aatcgtaaaa ctgatggcgg accgtgaagt ccgccgtgaa caggtcgccg
      301 tgaaggtcgg cgaacggttc atgctggtgc aggccgagga aatcatcttc gcctcgctgg
      361 ccgacgagtc gattaacata gtgacagggc aggtatcggg gacgtccaac tatcggactc
      421 tggacgactt acaggctcgg ctggatccgg cggtcttctg gcgcgtgcac cggtcccacc
      481 tggtcaacat caacaagatc aaggagattg tcccttggtt cagcaggaac tacatccttc
      541 ggatgaagga catgaagggc acggaaatcc cggtgagccg gtctcaaacg aagcggctcc
      601 gggaatacct gaagctctga accccgccgt atttgaaacc gccgaggctc agcccgctcc
      661 attcgagacg ccgatcagcg acgccccgcc cgccatcggt gacgtctcgg aaccgatggc
      721 ggcggccgcg cagatgcgga tggacctcga ggaccccgcg caagtcgtac ttcagcggct
      781 gaacgtctgg aaagacgaac tgattgcctg gttcaagacg ctcgtctcag cggcggtcta
      841 cgccacgctg atcgtcacgt tcggcttcca ggtcgcgcgc gtcgaaggcc agagcatggc
      901 gccgacgctc tcggaccagg atcggttgat cgtcaacaag ttcgcgtacc ggatcggggc
      961 gccgcgccgg ggcgacatcg tgatgctcta ctacccgatc aatcccgaca agtcgttcgt
     1021 caagcgagtc atcgccgagg aaggcgacac cgtgcgcatc gtcgacggcc gggtgtacgt
     1081 gaacgacgtg ccgatggatg acagcttcgt gccgcccgag ttccgcagtc atgacgacta
     1141 cggccccgaa gtgatcaagg aaggctatta cttcgtgatg ggcgaccacc gtaacaacag
     1201 ctccgacagc cgccactggg gcctggtgcc gaagaagtac atcatcggca aagtgcagct
     1261 tcgctggtgg ccgctgccac atgcgcacgt gttctgaatt cagaattcag aatccagaat
     1321 gcagacacgg tgtgtttgca ttctgaattc tgccttctgc atttccggtg aacgatcgct
     1381 acggccgcgt cgccaccgtc ctctggcggg tgatgttcct caacctcgcc gtggcgctcg
     1441 ccaagatcgg cctcgggcac tactcaggca ccgtctcaat catctccgag ggttaccact
     1501 ccctgaccga caccgccggc aatatcgtcg gcctcattgg cgtgtcgatc gcgcgccggc
     1561 cgccggacga gaatcacccc tacggtcacc gcaaatacga aacgatggcc tcgatcggga
     1621 tcatggtgtt cctgatcgtc gtcctggtgc aggtcctgag caggtccgcg gaccgcttcc
     1681 tccacggcgg aacaccgcac gtgtttcccg agggaatcgc gttgatggcg atcacgctcg
     1741 tcatcaacat cctggttgtc tcctatgaat cgcgggaagg gcggcggctg aagagcgaag
     1801 tgctgctcgc cgacgcccgg cacacgcgca gtgacgtcct cacgtcgacc gcggtgctcg
     1861 gcgcactgat tggcgtctgg gccggttatc cgattctcga tccgatggcg gcgctgctcg
     1921 tggcgatctt catcggccac gcgtgctgga ggatcgcgca ggaggcctcg cccattctcg
     1981 gcgacgagat gatgatggac gtccacgagg tgcagcaggt agtgcagagc gtcccgggcg
     2041 tgctcgggtc cgagaagatt cgcgcgcgcg ggtccgccga ctacgtgttt ctcgatctcc
     2101 acctctggct cgatccgcaa acaccgctct tcgacgcgca ccagacatcg cacgtcgtca
     2161 aggacacgct gatggccaag ttcccgcagt tgatggacgt ggtgatccat atcgaaccgc
     2221 cgccttcggc cgcggctgac aactcccaac ccccaacccg aaagagctga cccaacactc
     2281 ccgaccgcac tttcaccagc tcccaacggt tccgccgtga agggttggga gggttccatt
     2341 tgggagttgg gactcgtcct tttgatcggt tgggaattgg tggtttcgtg tgctcggcat
     2401 tccccttgct ccaccgtgcg ggcatggagc ctcagatccc gcagaccgaa tcgctggcca
     2461 gcctcgtacg cggtgtcctg atggatctac ggacgctcat ccgcgaggaa atttcgcttg
     2521 cccgcgtgga aatccgggag caggccaccc gcgcacgcgc cgcggcgatg agcttcggca
     2581 tcgcggcggg agcgctcgtc ttcggcggca tcttcctgct gatcgcggtg gccatggggc
     2641 tggccgatct gctcaactgg cccgtctggg caggtttcct gatcgtcgcg atcgtgctcg
     2701 gcctggttgg tgcgttcacg ctgtcatcgg gccgcaggca gctccgggcg ttccgcgccg
     2761 ttcctgaaga aaccgtcacg actctcaagg agaattccga atggatcgcc aggcggctgt
     2821 catccgggca gagatgagtg aaacgcgggc ggagctggat cgcaagatct cccggctcga
     2881 agcccgcgct cgcgagttca cgccgcaaca gctcaaggac cgctatgtcc ctgaatactt
     2941 cctcgaccgc gccattggcg ttgcgctcac gctgatcggc gtcggtctcg cgttcggggc
     3001 ctggcagcgc cgccgtaccc gccgcgacac cgtacgcacg atcgcggcgg cgtacgggtg
     3061 ctggtagcga tccctcgggc gtcccgtcga tagcggttcg ggtacgaatc cggtatcatg
     3121 agccgctttt tcggccattt tcacggctcg aggggacgcc agtgaacaca aacgacccgc
     3181 gcgaaatcat cgatcaatcc cccatgacgg tcgcccagat cgtcgtggtg attctcacgg
     3241 tctgcatgaa cggcatcgac ggtttcgacg tgctgtccat tgcctactcg tcccccggca
     3301 ttgccaagga atggggcatc gcccagacag ggctcggcgt cgtgctgtcg atggagctca
     3361 tcgggatggc gatcggatcg gtcctgctgg gcggcgtcgc ggaccggatt ggtcgacggc
     3421 cgacgctgct ggcctgcatc atcgccatgg tggtcggcat gctcggcgcg acgacggcgg
     3481 cgagcccact gcagctctcg atgtggcgcg tcttcaccgg cctcgggatc ggcggcatgt
     3541 tgtcggccat caatgccgtg gtcgctgaat tctccaacaa gcggtggcgg agcctctcga
     3601 tttcgatgat ggtgctcggc tatccgctcg taggggcgac cggcgggatg gtcgcgtcgg
     3661 cgctgctgaa gacccacgat tggcgctcgg tgttctacct cggcgcgacc gggacgctgg
     3721 tgttgctccc cgcggtgtac ctgctgatgc cggaatcgat tcactggctg gcgcggaagc
     3781 agccggcaaa cgcactcgcg cggatcaacg cagcgctggc gaagattggc catcgatcga
     3841 tcagcgcggt cccgcaggtg ctcgagtccg agcgcaagaa gtcggtggcg gacatctttt
     3901 caccggcact cggcgtcgtc accgtcatgg tgaccgccgc gtactttttc cacatcgtca
     3961 cgttctattt cctgctcaaa tggacgccga agatcgtcgc ggacatgggc tttgcgccct
     4021 cgtcggcagg cagcatcctg gcctgggcca acgtcggcgg cgcgcttggt acggcgctct
     4081 tcggtgtgtt gacggctcgc gtgggattga agcgcctgag tatcgtcatc ctgctcctga
     4141 gcgccgtggc gatcgccgtc ttcggccgga cgccgcagga gctcggcacg atggcatggc
     4201 tcgcggccat cgccggcttc ttcggcaatg caggcgtctc gggcctgtat tcgattgccg
     4261 cctacgcctt cccgacgcat gtgcgcgcca ccggcaccgg cttcgtgatt ggggtcggac
     4321 gcggcggcgc cgtgctcgca ccgattctgg ccggctacct gctccagacc gggttcccgc
     4381 tgccgactgt agcgctcatc atgggcgtcg ggtccctgct cggagcggtc gtactgatgt
     4441 tcctgaagct cgacgatcag cggccggtgg ccgagcgcgc gccgcagatg cgcacctcca
     4501 ccgcgcgcgc gtagcagcgt aac
//
LOCUS       DASSGX010000524         2945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_310743, whole genome shotgun sequence.
ACCESSION   DASSGX010000524 DASSGX010000000
VERSION     DASSGX010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310743"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..825
                     /locus_tag="VFI12_09440"
     CDS             127..825
                     /locus_tag="VFI12_09440"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7056673.1"
                     /translation="MASERVLIVDDEPQIRRALQTALSGHGYLVDIAGDGEAALTQVA
                     TRPPDAIVLDLVMPGSDGFAVLREIRGWSDVPIIVLSARGQEQDKVAALDLGADDYLT
                     KPFGMAELLARVRVVLRRRVTRHETRIAFGDVTIDLAARVVTRANAEVHLTPREYDLL
                     RALAADADKVLTHRQLLERVWGGYATENAQQLRVYINYLRRKLEDDPARPRWIVTEPG
                     VGYRLRTTSQGITA"
     gene            986..1822
                     /gene="tatC"
                     /locus_tag="VFI12_09445"
     CDS             986..1822
                     /gene="tatC"
                     /locus_tag="VFI12_09445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HET7056674.1"
                     /translation="MARALSHLPHPPRPHRPHRPVWLRPDDGTPEIFEEMTLGEHLIE
                     LRTRVVRACLSVGLAFIVGLIAAKPMLDRIRNDSLAKNGFDIRSPVDPLTIYFKVALY
                     IAVALAFPLIVYQVLAFVAPGLTRREKRVVYTALPFVALLAILGGAYGLLIAAPRALY
                     FLSHFMPSIFQWQPDADMIITFYLTLMLGLALAFQIPVVMFILAKIGIVSPRRMASIR
                     KYAFIVILILAAIITPSTDPFNMMIVAVPLYVLYEFGIVMARLFAKTPIISIPPPEPG
                     PA"
     gene            complement(1908..2135)
                     /gene="tatA"
                     /locus_tag="VFI12_09450"
     CDS             complement(1908..2135)
                     /gene="tatA"
                     /locus_tag="VFI12_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HET7056675.1"
                     /translation="MIGDVGWQELAIVLVIVVIIFGAGRLPEIGGAVGKSIKEFRAQS
                     SDDPPEPVQAAADATPSPRVAASALIRPDEI"
     gene            2601..>2945
                     /locus_tag="VFI12_09455"
     CDS             2601..>2945
                     /locus_tag="VFI12_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056676.1"
                     /translation="MPSENSERRFDVDAITAQNASRRSLIANGLRLSAVAGVAAATGA
                     SWSTARAQDATPSAMMSATPTADVSMIGGEPVDPQMQEVLKAFLSFGAPPISEVAYDV
                     ARNLPSFANAVAL"
BASE COUNT          492 a         1025 c          911 g          517 t
ORIGIN      
        1 gctccggcct cggtctcgcc atcgcccgcg gcttcatcga ggcgcacggc ggccggatct
       61 gggctgagag ccggcctggc gcggggacga gcgtccgctt caccatcccg acgaacggag
      121 cacgcagtgg cgagtgagcg agtgctgatc gtcgacgacg agccgcaaat ccggcgcgcg
      181 ctgcagaccg cgctctccgg tcacggctac ctcgtcgaca tcgccggcga cggagaagct
      241 gccttgaccc aggtcgcaac ccgtccgccc gatgcgatcg tgctcgacct cgtcatgccc
      301 gggagcgacg ggtttgcggt cctgcgcgag atccggggct ggtcggacgt gccgatcatc
      361 gtcctctccg cccggggtca ggagcaagac aaggtcgccg cgctcgacct gggcgcggac
      421 gactacctga ccaagccatt cggtatggcg gagctcctgg cccgggtccg ggtcgtcctg
      481 cgccgccggg tcacccggca cgaaacgcgg atcgcgttcg gcgatgtcac catcgacctt
      541 gccgcccgag tcgtgacccg tgccaacgcc gaggttcacc tgacgccgcg tgaatacgac
      601 ctgctccgcg cgctggccgc cgacgccgac aaggtgctga cgcatcggca actgctggaa
      661 cgcgtttggg gcgggtacgc gaccgagaac gcccagcagc tccgggtcta catcaactac
      721 ctgcgccgca agctggaaga cgaccctgct cgcccgcgct ggatcgtgac cgagcccggc
      781 gtcggctatc gccttcggac cacgagccag ggaatcactg catagagacg ctggcgtaat
      841 ctccgccgcg cccggggaaa actgccagca ccgccggcga cgtattctcc ggcaggtacc
      901 gggttgtgcg cgggcgcggt gcccgccgcg accggaatgc ggacgctgcg actcggcgcc
      961 ttcgccagcg atacaagggg agcccatggc ccgcgcactg tcccaccttc ctcatccccc
     1021 gcgaccgcac cggccccatc gcccggtctg gctacggcca gacgatggca cgcccgaaat
     1081 cttcgaggag atgaccctcg gggagcacct gatcgagctg cgcacgcgag tggtccgggc
     1141 ctgcctgtcc gtcggcctgg ccttcatcgt cgggttgatc gcggcaaaac cgatgctcga
     1201 caggattcgc aacgactcgc tggccaagaa cggcttcgat atccgctctc ccgtcgatcc
     1261 gctcacgatc tacttcaagg tcgcgctcta catcgccgtc gcgctcgcct ttccactgat
     1321 cgtctaccag gtgctggcat ttgtcgcgcc tggcttgacg cgcagagaaa aacgcgtggt
     1381 ctacaccgcc ctgcccttcg tggcgctcct ggcgatcctc ggcggggcct atgggttgct
     1441 gatcgccgcg ccgcgagcgc tgtatttcct cagccacttc atgcccagca tcttccaatg
     1501 gcagccggat gcggacatga tcatcacctt ttacctgacg ctgatgctgg gactagccct
     1561 ggcattccag attccagtcg tgatgttcat cctggccaag atcgggatcg tcagtcccag
     1621 gcgcatggcg tcgattcgca aatacgcttt catcgtaatc ctgatcctgg ccgccatcat
     1681 cacgccgagc acggacccgt tcaatatgat gatcgtggcg gtgccgctct acgtgctcta
     1741 cgagttcggc atcgtgatgg cccggctctt tgccaagacc ccgatcatct cgatcccgcc
     1801 gccggagccc ggcccggcct gagcacaatt cagcgtcgag tgacggggcg gatcgcaccg
     1861 caaacggagc cccgggcggg ccgcccgggg ctccgctgaa aggtcggcta gatctcgtcg
     1921 gggcggatca atgccgaggc ggcgacccgt ggcgacgggg tcgcatcggc cgcagcctga
     1981 actggttcgg gcggatcgtc actggattgg gcgcggaact ccttgatgct cttgccgacc
     2041 gcgccgccga tttccggcag gcgcccggcc ccaaagatga tcacgacgat tacgagcaca
     2101 atggcgagct cctgccaccc aacatcaccg atcatgtact gtttcttctc ctgccttggc
     2161 cgacatcgac cggaggctgt tagcgggtaa tcgcacgggt atacgccgcg caccctgatc
     2221 cgggtctcac ccggtcacgc agccattgcc tcctgctgct cccgatggat tgcgaccgtg
     2281 tccggcgttg gccaatgcct gcgcgcggcg cgtccgactc gctccgtccg gggtcgttcc
     2341 tggccgatcg cgccgcgcag gtccttgcca gagccacgta agtgaggccc caatgcactc
     2401 cgtcgcattg accaccagac acgactccta ttgtagcgaa gttcgcaggt aacctcatcc
     2461 gtgctacgga atgcagtgat ctaagcccct taggctcgac gaatacaccg cgatggggac
     2521 gacggacgtg cccaatcccg cacttgcgca gcgatgattg aacaattcat gcggttgcgc
     2581 gaattacaga aggagtagcg atgccttccg agaatagtga gcgtcgattc gacgtcgatg
     2641 ccattacggc acagaacgcg agtcgccgga gcctgattgc caacggcctc cggctctccg
     2701 cggtcgccgg cgtagccgca gccactggcg ccagttggtc taccgcgcgg gcgcaggacg
     2761 cgaccccgag cgcaatgatg agcgccacgc cgaccgccga cgtctcgatg attggcggcg
     2821 agccggtcga tccccagatg caggaagtgc tgaaggcatt cctctccttc ggcgctccgc
     2881 cgatctcgga ggtcgcgtac gacgtggccc gcaacctgcc cagcttcgcc aatgccgttg
     2941 ccttg
//
LOCUS       DASSGX010000525         3107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_638804, whole genome shotgun sequence.
ACCESSION   DASSGX010000525 DASSGX010000000
VERSION     DASSGX010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_638804"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="VFI12_09460"
     CDS             complement(<1..714)
                     /locus_tag="VFI12_09460"
                     /inference="COORDINATES: protein motif:HMM:NF024141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET7056677.1"
                     /translation="MGTFGWRIAPIYFLSVRQFVGGKAVRAVFALGLIPALFALIYRI
                     NPDIGRPRRFLDDIIIDVMLPSVLPLATLILATGAFGDEIEDRTLPYLVLKPITRVRI
                     VLEKLLASATVSIPIIAVGGVLSWFLVFTGDGGNNLDMLWGILAATAVGVVIYSAVFL
                     LMSLLIRRAILAAIIYSLVWESVLGRFIPGLRYLSIRHVVTSVYTSTIEALDPSGRLD
                     VGLGIDQAFSVQGALIGAVV"
     gene            complement(717..1682)
                     /locus_tag="VFI12_09465"
     CDS             complement(717..1682)
                     /locus_tag="VFI12_09465"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7056678.1"
                     /translation="MMPSPNGYQHLPGVIPGREPTIIVNRVSKWYGDIVAVSDITLGV
                     TPGVTALLGPNGAGKSTTLKMITGLLSPSSGKITILGKPARGNPGVYRTVGLVHEAEH
                     IYPYLTGREFVRINGILQRIPNLEQAVDRAIELVEMTYAQDRRIGEYSKGMRQRIKVA
                     AALVHDPGVVLMDEPLNGMDPAQRLHMINLIRELGRSKTVMVSSHILAEVERFAENII
                     VIVNGKLAAAGDYRAIRDRIDTHDHAIRIRASEPRFLAMALIGHPSTKGVRIDREDRV
                     VVETQDVRGFYLALPQLAQKANVRLFDISAADESLASVFSYLVEG"
     gene            complement(1693..2586)
                     /locus_tag="VFI12_09470"
     CDS             complement(1693..2586)
                     /locus_tag="VFI12_09470"
                     /inference="COORDINATES: protein motif:HMM:NF024741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HET7056679.1"
                     /translation="MADINVQPRYGEIYDRGYQHYLGKRLGRGHAIWALITYSMKRAM
                     GIKKSWTAKVIPFILYLAVIGTVVVVIGIEAFLKSAGTVAMNYPDYFTFIYLIEGAFV
                     ATIAPEMLCGDRRENVLALYFSRAISRLDYVLSKLAATAILTMTMSVVPVVFLWLFRQ
                     LLADKPLHALTTNIDDLGKVILVGSLIALYLGCGGLVISSFTGRKSIATAIIFIGYAV
                     LEGFTNALIEAFDSQRAKETLALISPANLLNGLAEKLFGTWDTFATGYPGYSAVVYVA
                     AALGIILIGILIMVWRYVPED"
     gene            complement(2579..>3107)
                     /locus_tag="VFI12_09475"
     CDS             complement(2579..>3107)
                     /locus_tag="VFI12_09475"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7056680.1"
                     /translation="GMKQRVKLAQALVHDPALVFLDEPTNGMDPQGRDDMLELIQRIY
                     RMLGIAVVVSSHILEDIERVCDHVVIIDEGKLVIDQPLTGMGTPQGDLLVRIDGNQEA
                     FVARMEAQGIAVRRPTDDDLQTSDELIVANIGEAVYDAVRDTAAELGVGLRSLRMKGR
                     SLEDVYLGEVGGGNG"
BASE COUNT          557 a          986 c         1051 g          513 t
ORIGIN      
        1 gaccaccgcg ccgatcaatg caccctgcac gctgaacgcc tggtcgatcc cgaggccgac
       61 atccagccgg ccgctggggt ccagcgcctc gatcgtcgag gtgtagaccg atgtgacgac
      121 gtgccggatc gagagatagc gcagccccgg gatgaaccgg ccgaggaccg attcccagac
      181 cagcgagtag atgatcgcgg ccaggatcgc gcggcggatc agcaggctca tcagcaggaa
      241 gaccgccgag tagatgacga cgccgaccgc cgtcgccgcc aggatgcccc agagcatgtc
      301 gaggttgttg ccgccgtcgc cggtgaagac gaggaaccag ctcaggacgc cgccgaccgc
      361 aatgatcggg atcgagaccg tcgccgatgc cagcagcttc tcgagtacga tccggacccg
      421 cgtgatcggc ttcaggacga gatatggcag cgtccggtcc tcgatctcat cgccgaacgc
      481 gcccgttgcc aggatgagtg tcgccagcgg caggaccgat ggcagcatga cgtcgatgat
      541 gatgtcgtcc aggaagcggc ggggccggcc gatgtccggg ttgatccggt agatcagcgc
      601 gaagagggcc gggatcaggc cgagcgcgaa aacggcgcgg accgccttgc cgccgacgaa
      661 ctggcggacg ctcaggaagt aaatcggcgc gatgcgccag ccgaacgtgc ccacggtcag
      721 ccctccacca ggtatgagaa gaccgaggcg agcgactcgt ccgccgcgct gatatcgaac
      781 agccggacat tcgccttctg cgcgagctgc ggcagcgcga gatagaagcc gcgcacatcc
      841 tgcgtctcga cgacgacccg atcctcgcgg tcgatgcgga cacccttggt gctcggatgc
      901 ccgatcagcg ccattgccag gaagcggggc tcgctggccc gaatccggat tgcgtggtcg
      961 tgtgtgtcga tccgatcgcg gatggcccga tagtcgcccg ccgcggccag cttgccgttg
     1021 acgatcacga tgatgttctc ggcgaagcgc tcgacctcgg ccaggatgtg cgacgagacc
     1081 atgacggtct tgctgcggcc cagttcgcgg atcaggttga tcatgtgcag gcgctgcgcg
     1141 ggatccatcc cgttcagcgg ctcgtccatc aagaccacgc ccgggtcgtg gaccagcgcc
     1201 gccgcgacct tgatccgctg gcgcatgccc ttcgagtact cgccgatccg ccggtcctgc
     1261 gcgtacgtca tctcgaccag ctcgatcgcg cggtccaccg cctgctcgag gttggggatg
     1321 cgctggagga tgccgttgat ccggacaaac tcgcgaccgg tcaggtacgg gtagatgtgc
     1381 tcggcctcat gaaccagccc aacggtccgg tagacacccg ggttgccgcg cgccggcttg
     1441 ccaaggatcg tgatcttgcc gctcgacggt gaaagcaggc cggtgatcat cttgagcgtg
     1501 gtcgatttgc cggcgccgtt cgggccgagc agcgcagtca cgcccggcgt gacgccgagg
     1561 gtgatgtcgg agacggcgac gatatcgcca taccacttcg agacgcggtt gacgatgatc
     1621 gtcggctccc gccccgggat cacgccgggc aggtgctggt atccgttcgg cgacggcatc
     1681 atgggcgggg cctcagtctt ccgggacata gcgccagacc atgatcagga tgccgatcag
     1741 gatgatcccg agagcagcgg ccacgtagac cacggccgag taaccgggat aaccggtggc
     1801 gaaggtgtcc caggtgccga acagcttctc ggcgagcccg ttgagcaggt tggccgggct
     1861 gatcagcgcc agggtctcct tggctcgttg cgagtcgaac gcctcgatca gcgcgtttgt
     1921 gaagccctcc agcaccgcgt agccgatgaa gatgatcgcg gtggcgatgc tcttgcggcc
     1981 ggtgaaggag gagatgacga ggccgccgca gccgagatag agcgcgatca acgagccgac
     2041 gaggatcact ttgccgaggt cgtcgatgtt cgtggtcagc gcgtggagcg gcttgtccgc
     2101 cagcagttgc cggaaaagcc agagaaacac gaccggcacg accgacatgg tcatggtcag
     2161 gatggccgtg gccgccagct tgctgagcac gtaatcgagc cgggagatgg cccgcgaaaa
     2221 gtagagcgcg aggacattct cgcgccggtc gccgcagagc atctccggcg cgatcgtggc
     2281 cacgaacgcc ccctcgatca gatagatgaa ggtgaagtag tcggggtagt tcatcgcaac
     2341 ggtgccggcg ctcttcagga acgcctcgat cccgatcacg accacaaccg tgccgatgac
     2401 ggcgaggtag aggatgaagg ggatgacttt ggcggtccag ctcttcttga tccccatcgc
     2461 ccgcttcatc gaataggtga tcagggccca gatcgcgtgg ccgcgcccga gtcgcttgcc
     2521 gaggtagtgc tggtagccgc gatcgtagat ctcgccgtag cgcggctgga cgttgatgtc
     2581 agccattgcc gcctccgact tccccgaggt agacgtcttc gagcgagcgc cccttcatcc
     2641 gcagggagcg caacccgacg ccgagctcgg ccgcggtgtc gcggaccgcg tcgtagaccg
     2701 cctcgccgat attggcgacg atcagctcgt cgctggtctg gaggtcgtcg tcggtcggcc
     2761 ggcggacggc gatgccctgc gcctccattc gggcgacgaa tgcctcctgg ttgccgtcga
     2821 tgcgaaccag cagatcgccc tgcggcgtgc ccatcccggt cagcggctgg tcgatgacga
     2881 gtttgccctc gtcgatgatc acgacgtggt cgcagacgcg ctcgatatcc tcgaggatgt
     2941 gcgacgagac gacgacggcg atcccgagca tccggtagat gcgctggatc agctcgagca
     3001 tgtcatcccg gccctgcggg tccatgccgt tcgtcggctc gtcgaggaag accagggccg
     3061 gatcgtggac cagcgcctgg gccagcttga ctcgctgctt catgccg
//
LOCUS       DASSGX010000526         4009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_747517, whole genome shotgun sequence.
ACCESSION   DASSGX010000526 DASSGX010000000
VERSION     DASSGX010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4009
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747517"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VFI12_09480"
     CDS             <1..403
                     /locus_tag="VFI12_09480"
                     /inference="COORDINATES: protein motif:HMM:NF018533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HET7056681.1"
                     /translation="RTAAIKALILDQRIIAGVGNIYADEALFRSRIHPLTPGGELSTA
                     QVASLVRSIKGVLRSGIEHRGTTFSSYRDANGEAGGNQFRLRVYGRGRRGEPCPRCGQ
                     PLVTLVAGGRTSHVCLHCQPLPASADSAATR"
     gene            complement(315..956)
                     /locus_tag="VFI12_09485"
     CDS             complement(315..956)
                     /locus_tag="VFI12_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056682.1"
                     /translation="MHTIDRSTSFWLNMAVKVALVGLLLFAVLNQDMQQFQGKAMTGR
                     ALTYPIAIIIVPVVFWLLRRSRPGLGYPHLLDMLLALPFLIDTAGNALDLYDTIDWWD
                     DANHFVNWAILCAAFGQLLLRFPYGRWTMAGLIVGFGSVSGVIWEIAEYFTFVRGGPE
                     EATAYTDTLGDLSLDLGGAVVAAWVTAWLLNRPKLEAAGSADKRGLSDLRQPA"
     gene            1119..1421
                     /locus_tag="VFI12_09490"
     CDS             1119..1421
                     /locus_tag="VFI12_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056683.1"
                     /translation="MFGTIGRARPKPGVKAEFEAAMEDWKREIRPRLPGHFLEMTGTS
                     VDHPDEIMFIALAQDEKTYRALAEMPEQHAFFERMSALVDGDIRWEDIEMEVSVDD"
     gene            complement(1431..2297)
                     /locus_tag="VFI12_09495"
     CDS             complement(1431..2297)
                     /locus_tag="VFI12_09495"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET7056684.1"
                     /translation="METRRLGRTGHESTVVTLGGYAFGVLDQDGADALIEAAFGRGVN
                     QIDVAPSYADAEVRLGDYLQRHPHPMPDLFISCKTQQRARAQAWDELHQTLARLGRDQ
                     LDLYQLHAVCTMADLEACFAEGGSMEALIDAQRAGLVKSIGITGHGWDAPATHLAALD
                     RHPFDTVMTSANLFMVQNEDFRRDWDRLLDRCEQEDVGVHLLKATAKTSWGDRPHDYN
                     TWYEPFTAADDVNRAVAWALNQPIDTICSAGDRSLFETICDAAERYDTIDEAQQRTLL
                     EQRNYGDIFVSS"
     gene            complement(2382..3545)
                     /locus_tag="VFI12_09500"
     CDS             complement(2382..3545)
                     /locus_tag="VFI12_09500"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HET7056685.1"
                     /translation="MTAGNVAVGDDPGLVEAEHAFALTADLVRIRSYPGEEAAVQAYV
                     ADWFRSNGLAPDVEIVAPDRPNVLVRVENGPGPVFLLNGHVDTVLADPGWAHDPWQGR
                     RDGDRFYGLGACDMKSGVAAAMLATRALARNRDRWSGTVLFTSVVDEEAYSIGANALI
                     DSGIKADYCVVTEASWELPCLGSFGKYLVRVDVTGRGAHASWPERGTNAAVEAAKLVA
                     RLDEMQLPRHARIRPSQCVLSLLSGSPQYVITVPDRATILINRHTIPGETEESVLAEY
                     QALIDGLDSPATFTLSIDPPRYPSWETPESHPLVQAFAAAYTDEAGHEPNFGYTGYGD
                     PNLFSTVAGIPTVMFGPLGGNYHEANEWVDISSIAGTARVLERLVQDMLPPHV"
     gene            complement(3542..>4009)
                     /locus_tag="VFI12_09505"
     CDS             complement(3542..>4009)
                     /locus_tag="VFI12_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HET7056686.1"
                     /translation="PMSYHNGSIWPHDNSMIGAGFYRYRFKEAGHAVATALFDVAQDE
                     DLFRLPELYCGFGRHGNFEERPIGYPVSCSPQAWAAGAVPLIVTSMLGMEVDLANRRL
                     AISPSLPKWLDEVTIHDLAVLGSRGTLTVRRSGMGYSVDAVALPLQTPERARA"
BASE COUNT          744 a         1304 c         1275 g          686 t
ORIGIN      
        1 ccgaaccgcg gcgatcaagg cgctgatcct cgaccaacgg atcatcgccg gcgtcggcaa
       61 tatctacgcg gacgaggcgc tgttccggtc ccggatccac ccgctcacac ccggcggcga
      121 gttgtcaacc gcgcaggtgg cgagcctcgt ccggtcgatc aagggcgtac tgcggtccgg
      181 gatcgagcac cggggcacga ccttctcgtc ctaccgggat gccaatggcg aagcgggcgg
      241 aaaccagttc cgactgcgcg tatacgggag gggccggcgc ggggagccct gcccccgctg
      301 cggccagccg ctggtcacgc tggttgccgg aggtcggaca agccacgttt gtctgcactg
      361 ccagccgctt ccagcttcgg ccgattcagc agccacgcgg tgacccatgc ggccaccacc
      421 gcccctccca gatccagcga gagatcgccg agcgtgtcgg tgtaggccgt tgcctcctct
      481 ggaccgccac gcacgaaggt gaagtactca gcaatctccc agatcacgcc tgacacgctg
      541 ccgaagccga caatcagacc ggccatcgtc cagcggccat atgggaaccg aagcagcaac
      601 tggccgaatg cggcgcagag gatcgcccag tttacgaagt ggtttgcgtc gtcccaccag
      661 tcgatcgtgt cgtagaggtc gagcgcatta cccgcggtgt caatcagaaa cggcagggcg
      721 agcagcatgt caagcaggtg cggatagcca aggccgggtc gcgacctgcg caagagccag
      781 aacacgaccg gtacgatgat gatggcgatc gggtaggtca gcgcccggcc ggtcatcgct
      841 ttgccctgga attgctgcat gtcttgattg aggacagcga acaggaggag gccgaccagc
      901 gcgaccttga ccgccatgtt gagccagaac gaggttgacc tgtcgatcgt gtgcacgtgc
      961 tgtctccccg atccgaatcc acacggtgat tcttcacgaa attatcgtcc ggggcctttg
     1021 tcaatcggct gacgagcccc tatttacatc cgcatggatt ccgctaatct tggctaggct
     1081 aaacagtcgg atatcagcaa ttcacaggag gaacggcgat gtttggaaca atcgggcgcg
     1141 cccgcccgaa gcccggcgtg aaggccgagt tcgaggcagc gatggaggac tggaagcggg
     1201 aaatccgccc ccggcttccc ggacactttc tggagatgac gggcacatcc gtggaccacc
     1261 cggatgagat catgttcatc gcgctggcgc aggacgaaaa gacttaccgg gccttggccg
     1321 aaatgccgga gcaacacgcc ttcttcgagc ggatgtccgc gctcgtcgat ggcgacatcc
     1381 gctgggaaga catcgagatg gaggtcagcg tcgacgattg atcgtcaggc tcacgagctg
     1441 acgaaaatat cgccgtagtt tcgctgctcg agaagcgtgc gctgctgtgc ctcgtcgatc
     1501 gtgtcgtaac gctcggcggc gtcgcagatc gtctcgaaga gtgagcgatc gccggcgctg
     1561 cagatcgtat cgatcggctg gttcagcgcc caggcaacgg cgcgattcac gtcgtcagcc
     1621 gcggtgaatg gctcgtacca cgtgttgtag tcgtgcggac gatcgcccca cgatgtcttc
     1681 gcggtcgcct tcagcaggtg aacgcccacg tcctcctgtt cgcatcggtc gagcagtctg
     1741 tcccagtcgc ggcggaagtc ttcgttctgg accatgaaca agttggctga ggtcatgaca
     1801 gtatcgaagg gatgccgatc aagcgcggcc agatgggtcg ccggcgcatc ccaaccgtgc
     1861 ccggtgattc caattgactt gaccaggccg gcgcgctgcg catcgatcag cgcctccatc
     1921 gaaccgccct ctgcgaagca cgcctccagg tccgccatgg tgcaaaccgc atggagctga
     1981 taaagatcga gctggtcccg gccgagacgc gcaagggttt gatgaagctc gtcccacgcc
     2041 tgggcacggg cccgctgctg cgtcttgcaa ctgatgaaga gatccggcat cggatgcgga
     2101 tgccgttgga ggtaatcgcc caggcgaacc tcggcatccg catagctcgg cgccacgtcg
     2161 atctgattga cgccccggcc gaacgccgcc tcgatcaggg catcggcgcc gtcctggtcg
     2221 agcacaccga aggcgtagcc accgagagta acgacggtac tctcgtgtcc ggtccgtccg
     2281 agtcgtcggg tctccatcac gctcctcctg ccgggttgct ggctttcgtc atactgtagg
     2341 gcgatccgcg gaagccgtgg gaccggacga gttccgccgg actacacgtg aggcgggagc
     2401 atatcctgca ccaacctctc caggacccgg gcggtgcccg cgatgctcga aatatcgacc
     2461 cactcgttgg cttcgtgata gttgccgccc agcgggccga acatgacagt cgggatgccc
     2521 gccacggtcg agaagaggtt cggatcgccg tagccggtgt acccgaagtt tggctcgtgg
     2581 cccgcttcat cggtataggc ggcggcgaat gcctgaacga gcggatgcga ttccggcgtc
     2641 tcccacgatg ggtaccgcgg cgggtcgatc gagagcgtga acgttgcggg cgaatccaaa
     2701 ccatcgatca gcgcctggta ctcggcgagg acgctttcct ccgtttcgcc ggggatcgtg
     2761 tgccggttga tcaggatggt tgcgcggtcg ggcacggtga tgacgtactg cgggctcccg
     2821 ctcaacagcg aaagcacgca ctgggacggg cgaattcggg catgccgcgg gagctgcatc
     2881 tcgtcgaggc gcgctacgag cttcgccgcc tcgaccgccg cattggtccc ccgctccggc
     2941 cacgaggcat gcgcgcctcg tcccgttacg tcaacccgga ccaggtactt gccgaacgag
     3001 ccgaggcatg gcagctccca actcgcctcg gtcacgacac agtaatcggc cttgattccg
     3061 gaatcgatga gcgcgttggc gccgatcgaa taggcttcct cgtccacgac cgacgtaaac
     3121 aggaccgtcc cgctccagcg atccctgttc cgggcaagcg cgcgcgtcgc cagcatggcc
     3181 gccgcgactc cgctcttcat atcgcacgcg ccgagaccat agaaccgatc gccgtcacgc
     3241 ctgccctgcc acggatcgtg tgcccaaccg ggatcggcca ggaccgtgtc gacgtgccca
     3301 ttgagcagga agaccggtcc cgggccattt tcgacccgca ccagcacgtt cggacggtcc
     3361 ggtgcgacga tctccacatc cggcgcaagg ccgttggacc ggaaccagtc ggcgacatag
     3421 gcctgaacgg ccgcctcttc gccgggatag ctgcggatcc ggaccaggtc ggcggtcagc
     3481 gcaaacgcat gttccgcctc gacaagcccg ggatcatcgc cgactgccac attgccggcg
     3541 gtcacgcgcg cgcccgttcc ggcgtctgga ggggcagagc caccgcgtcg actgagtagc
     3601 ccataccgga gcgacggacc gtcagcgtgc cccgcgatcc cagcactgcc aggtcgtgga
     3661 tcgtgacctc gtcaagccac ttcggcagcg acggcgagat ggcgagtcgc cggttcgcca
     3721 ggtcgacctc catgccaagc atcgaggtga caatgagcgg gaccgcgccg gctgcccagg
     3781 cctgcggcga gcagctcacc gggtacccga tcggccgttc ctcgaaattc ccatgccgcc
     3841 cgaagccgca gtacaactcc ggcagccgga acaaatcttc gtcctgggcg acatcgaaga
     3901 gggcggtcgc gaccgcatgg ccggcctcct tgaagcggta ccggtagaag ccggcgccga
     3961 tcatgctgtt gtcatgcggc cagatcgagc cgttgtgata actcatcgg
//
LOCUS       DASSGX010000527         2825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1620267, whole genome shotgun sequence.
ACCESSION   DASSGX010000527 DASSGX010000000
VERSION     DASSGX010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1620267"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VFI12_09510"
     CDS             complement(<1..227)
                     /locus_tag="VFI12_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056687.1"
                     /translation="MPDQTQPVTGDVWKDQQLVTAFLTGVRAALPNASDQLEVMLRVL
                     IFSGRPIRRVLDLGSGSGVLAGAILERFPDAV"
     gene            complement(483..974)
                     /locus_tag="VFI12_09515"
     CDS             complement(483..974)
                     /locus_tag="VFI12_09515"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HET7056688.1"
                     /translation="MPIRDERPADVDGIHALLDQAFPGEPVGRLVDELRTEGDLLISL
                     VTEVDGQVAGHVGFSPIAIAPCLMRAVQLSPLAVAESHRRQGIGAELVRTGIDRCRSL
                     GIEAVLVLGDPAYYRRFGFDPAAAAHLRSRWSGPHLMALFLTDRSLSACTFLSLAPAF
                     ERL"
     gene            complement(980..1486)
                     /locus_tag="VFI12_09520"
     CDS             complement(980..1486)
                     /locus_tag="VFI12_09520"
                     /inference="COORDINATES: protein motif:HMM:NF018590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HET7056689.1"
                     /translation="MATLFAVVYPSKEQATSAFDQAKALENAGYMKIMEQALISKDDS
                     GAIQIGDENHPVLKGAGIGAVVGVLTGAIFLIPVAGLALGAAVGSVLGRFGKSGAEDD
                     FKKFQQTVSTELQPGGGAMLLLIQTDSPDRVVNDLGHFGGKLISYDLTDTQLAELQKE
                     IDKLSAQK"
     gene            complement(1943..>2825)
                     /locus_tag="VFI12_09525"
     CDS             complement(1943..>2825)
                     /locus_tag="VFI12_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056690.1"
                     /translation="NTSGDVSLGNTISGDSTSTVTSNPNDAGNGVGGDGTGGNSSAAD
                     GFGGNGAGGDATQEVSAVGQHVNAGGNHAGDGGESTGGTATAGDGTGGMGGPAAGGAG
                     DGGNGGDGGNGGNGGAANSGDAVGGDGGNAQVWSTSSDGSDVEPLYLGLCADDVDFNC
                     GWHEVDGSCTGTCKGPIAGDCTGFCFGEVEGTCTGVCIDGHHHGGHDHDGDHDGGGDW
                     NGGGGWNHGGHGGWWPNKGWGGKSYAGTGGSTVSSIKALPTTGAGNSTGDSNELMLLG
                     VVALLGLAAAGLRKRTV"
BASE COUNT          481 a          880 c          971 g          493 t
ORIGIN      
        1 acggcgtcag gaaagcgctc gagaatggcg ccggcgagca ccccggagcc gctaccgaga
       61 tcgagcacgc ggcgaatcgg ccgaccggag aagatcagga cccgaagcat gacctcgagc
      121 tgatcggagg catttggcaa ggccgcgcgg acaccggtga gaaaggcggt gaccagctgc
      181 tggtccttcc agacatcgcc ggtaacgggt tgggtttgat cgggcatgga tgcgctccga
      241 gaacgaggtc aatcgtcacg ggagtgtagc aatctgattt gcgttccgga ttcgtatggc
      301 cgaggagcgg gatagccgtt gacccaacta cttcgagccg ggtctaaatc gtccgcatgc
      361 gtgtggagtg aacctcgcca caacgcgaca cggactgtct tgaggttgga gatgcagccc
      421 tctgtggctg cttcgagccg cggagggcag ccacagaggg ctgcatctcc gcgacgaacc
      481 gcctacaggc gctcgaaggc cggagcgagg gaaagaaagg tgcaagcgga gagcgatcga
      541 tcggtgagga acagcgccat caggtggggg ccggaccagc gggaacggag gtgggccgcg
      601 gccgcgggat cgaacccgaa gcggcggtag tacgccgggt caccgaggac gaggacggct
      661 tcgatgccca gggagcggca gcggtcgatg ccggtgcgga caagctctgc accgatcccc
      721 tgccggcggt gcgactcggc caccgccagc ggtgacagct ggacggctcg catcaggcac
      781 ggggcgatgg cgatcgggct gaagccgacg tggccggcga cctgcccatc gacctcggtg
      841 acgagtgata tgagcagatc tccctcggtg cgcagctcat cgacgagccg gccgaccggt
      901 tcgccgggga atgcctggtc gagcaaggca tggatcccgt cgacgtcggc cggccgctcg
      961 tcgcgaatgg gcatacgggc tatttttggg cagagagttt gtcgatttcc ttctgcagct
     1021 cggccagctg cgtgtccgtg aggtcgtaag agatcagctt gccaccgaag tgcccgaggt
     1081 cgttcacgac gcgatcgggc gaatcggtct ggatgaggag cagcattgca ccgccgccag
     1141 gttgaagctc ggtggatacg gtttgctgga atttcttgaa gtcgtcttcc gcgccgctct
     1201 tgccaaagcg gccgagcacg ctgccaacgg ccgcgcccag cgcgagcccg gccacgggga
     1261 tgagaaagat cgctccggtc agaacgccga cgaccgcgcc gatgccggcg cccttgagga
     1321 cgggatgatt ctcatcgccg atctggatcg cgccggaatc gtccttgctg atgagcgctt
     1381 gttccatgat cttcatgtac ccagcgtttt cgagcgcctt ggcctgatcg aaggcgctgg
     1441 tggcctgttc cttggagggg tacacgactg cgaaaagtgt cgccatgggt gtgctccagt
     1501 tcactcgatt gcgaataggt tcctcgttgc tttgctgagt gtacccggtt ggcgggtgag
     1561 attggcgatc gcagggcgag ggggccttgc cctatgagac gttggtgtgc gggagtttcg
     1621 gttgggatgc ggccgggacg ccggagataa atcgtccccg agacgggaag gcagcgacgc
     1681 ccttacctac gaccacgagc cccgtggtgg gaggcgagag gtcagagatg ccgtccgcaa
     1741 cgaccatttg acctgtggtg agcgccggga cggcagaggc gctcttttca ttgcgaccgc
     1801 tcgacccgtt tcgggcgggc agaaatgccc ttgcgcgacg attggcagga catgcaacaa
     1861 gggttccgga gcgatccgga acccttgttc tcactattcg ttgctcgacc agccccgccg
     1921 gagcggggct ggttcgcatg tgctagacgg tccgcttgcg gagaccagct gcagccagac
     1981 cgaggagggc cacgacgccg aggagcatca gctcgttgga gtcgcccgtg ctgttgccag
     2041 cgccggtggt cggcagtgcc ttgatcgacg aaaccgtgct tccgccggtt ccggcgtagg
     2101 acttgccgcc ccaacccttg ttcggccacc agccgccatg gccaccgtgg ttccaaccac
     2161 cgccgccatt ccagtcgcca ccaccgtcgt ggtcgccatc atggtcgtgt ccgccatgat
     2221 gatgaccgtc gatgcagacg ccggtgcagg tgccttcgac ttcgccgaag cagaagccgg
     2281 tgcagtcgcc tgcgatcggg cccttgcagg tgccggtgca gctaccgtcg acttcgtgcc
     2341 aaccgcagtt gaagtcgaca tcgtcagcgc agagaccgag gtagagcggt tccacatcgg
     2401 aaccgtcgct cgaggtcgac cagacctgcg cgttgccgcc gtcgccgcca accgcgtcgc
     2461 cgctgttagc cgcgccgccg ttgccgccgt tgccgccgtc gccgccattg ccgccgtcgc
     2521 cagcgccacc ggcagccggg ccgcccatgc caccggtacc gtcgccggcc gtggctgtgc
     2581 caccggtcga ttcgccgccg tcaccggcat ggttgccgcc ggcgttgaca tgctggccga
     2641 ctgccgacac ttcctgggtc gcgtcgccac cggcgccgtt gccaccgaat ccatcggccg
     2701 cgctggagtt tccgccagtg ccgtcgccgc cgacgccgtt gccggcatcg ttcggattcg
     2761 aggtgaccgt cgaagtcgaa tcgccgctga tggtgttgcc gagcgagaca tcgccggagg
     2821 tgttg
//
LOCUS       DASSGX010000528         2412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_858711, whole genome shotgun sequence.
ACCESSION   DASSGX010000528 DASSGX010000000
VERSION     DASSGX010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858711"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VFI12_09530"
     CDS             <1..498
                     /locus_tag="VFI12_09530"
                     /inference="COORDINATES: protein motif:HMM:NF017945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HET7056691.1"
                     /translation="IFAMISIHFPMTYGHAQGWLVLVAIMGISVWVRHFFNLRHRGRT
                     VWAIPISAAVAMVLLAIAISPAGRAGAGNPGGQPVPFTTVRQIIQLRCQTCHSSHPTR
                     PGFDAPPLGITFDTAQQIRDLAPRIEQMAVISKLMPLGNATGMTQDERDTLGRWIHQQ
                     ESDGK"
     gene            complement(505..1053)
                     /locus_tag="VFI12_09535"
     CDS             complement(505..1053)
                     /locus_tag="VFI12_09535"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7056692.1"
                     /translation="MSAAIGELRDVGVANSWRARPTRASRVTVINDDPEFLALMGDIF
                     SGDHFAATLLDEDGPDVLERASESKPDLLMIDLRSCADRVDGWKIARTLRRDPELARL
                     PILVCSGDYQAMSQLADQAANDTRLEFLRKPFGLDQLTDAIARLLGRHGIGHGMVSSS
                     RARSDSADRVDGWPGGEAVPTR"
     gene            1224..1994
                     /locus_tag="VFI12_09540"
     CDS             1224..1994
                     /locus_tag="VFI12_09540"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7056693.1"
                     /translation="MTVRSSTVDPSVQRALVLHTHQVVVDLIRLTLNHGMFVVRAAGS
                     MAEAEAILAEWQPHMAVVDMDHDDSFDLLVRLGASNTLERSDTPVLGLTRRGDLPTKL
                     KAFDLGVDDILVTPFSPAELLARSVVISRRASGVDRPIVPTITLGEIEIDIVKREVRA
                     GESVIHLTGIEQSLLYALASRNGRVVTREEILDGVWGTDFVAESNIVDRHIRSLRIKL
                     QNDYRHPRFIATVPGRGYKFIPTFSNTGWIAEDAGTAH"
BASE COUNT          385 a          820 c          800 g          407 t
ORIGIN      
        1 atcttcgcca tgatcagcat ccacttcccg atgacctacg ggcatgcgca gggctggctg
       61 gtcctggtcg cgatcatggg catctccgta tgggtgcggc acttcttcaa cctgcgtcac
      121 cgcggtcgca ccgtgtgggc gatcccgatc agcgccgcgg tcgccatggt gctgctggcc
      181 atcgcgatct cgcccgccgg gcgcgccggg gcggggaatc cgggcgggca accggtgccg
      241 ttcaccaccg tgcggcagat catccagctg cgctgccaga cgtgtcattc atcgcatccc
      301 acccgccccg gcttcgacgc gccgccgctg ggaatcacct tcgacacggc gcagcagatc
      361 cgcgacctgg cgccgcgcat cgagcagatg gcggtgatct cgaagctcat gccgttgggg
      421 aacgccaccg gcatgaccca ggacgagcgc gacacgctgg gccggtggat ccatcagcag
      481 gagtcggatg ggaagtgagc ggcctcacct cgtcggaacc gcttcgcctc cgggccaccc
      541 gtcgacgcgg tcagccgagt cggatcgtgc tcgcgacgag ctcaccatgc catggccgat
      601 gccatgacgc cccaggaggc gagcgatcgc atcggtgagc tggtcgagcc cgaacggctt
      661 gcggaggaac tccaggcgcg tatcgttcgc tgcctgatca gcgagctgcg acatcgcctg
      721 gtagtcgccg ctgcacacca ggatgggcag cctggccagc tcggggtcac gtctgagcgt
      781 ccgtgcgatc ttccagccgt cgacgcgatc cgcacaagac cgcagatcga tcatcaggag
      841 gtcgggcttc gactcgctgg cccgctcgag cacgtccggc ccatcctcgt cgaggagcgt
      901 ggcggcgaag tgatcgccgc tgaagatgtc gcccatgagc gccaggaact ccgggtcgtc
      961 gttgatgacc gtgacgcgcg acgccctcgt cgggcgggcg cgccacgagt tcgcgacccc
     1021 aacgtcccgc agctctccga tcgccgcgct cacctgggca ccggccagtc ctggtccatc
     1081 gccaaagttt ggtccacgtc gcctccgtag ccccacgctc ggtgggtttg cgtgatgcca
     1141 tcgtggcgcc cggtgggggc atggtcatta cgggtcgtca tcacggctcc tgacccgtcc
     1201 agggggatac aattgacttg accatgaccg tccgctcatc aacggttgac ccctccgtgc
     1261 agcgggcgct cgtactgcac acgcaccagg tcgtcgtgga cctgatccgg ctgacactga
     1321 accacggcat gttcgtcgtg cgggccgcgg gcagcatggc cgaggcggag gccatcttgg
     1381 ccgagtggca gccgcacatg gccgtggtcg acatggatca tgacgacagc ttcgatctcc
     1441 tggttcggct gggagcgtca aacaccctcg agcgcagcga cacgccggtc ctggggctga
     1501 cgcgccgcgg cgatttgccg accaagctca aggcgttcga cctgggagtt gacgacatcc
     1561 tcgtcacgcc gttctcgccg gcagagctgc tggctcgctc ggtcgtcatc tcccgtcgcg
     1621 cctcgggcgt cgatcggccg atcgtcccga ccatcacgct gggcgagatc gagatcgata
     1681 tcgtcaaacg cgaagtgcgg gccggcgaat cggtcatcca tctgaccggg atcgagcaga
     1741 gcctgctcta cgcgctcgcc agccgaaatg gccgggtcgt cacgcgtgaa gagatcctgg
     1801 acggggtttg gggtacggac ttcgttgccg agagcaacat cgtcgatcgc cacattcgca
     1861 gcctgcggat caagctgcag aacgactacc ggcacccgcg cttcatcgcg acggttcccg
     1921 gccggggcta caagttcatc ccgacgttct ccaacactgg ctggatcgcc gaggatgcgg
     1981 gcaccgcaca ctgaggaccg gcatcctgcc ccgggcgcat ctgcgcgtgt gagtggctcc
     2041 ggcgggcggg agccggccgt gtactgaatt cccttggtac aacctcgagc gagatctcaa
     2101 ttccccatcc caagcggtct cgtgggtggc tgacggtcgc gctccttgag tgcgcacggt
     2161 gccaccaagt cgtggagcgc gcatcgccga tgcagcgcca ctgtccgtcc tgtcgggccg
     2221 ccctgaagcg gcgtctcagc cgagcgtcga tggcgcgccg ccgcgggaac cagggcgacc
     2281 agtcaggacc cgagcagcgg gagatactcc tcgagcacct cagcgtagct gggcagcgat
     2341 gggcggatca gctcgatgtg ctcggccggg gtgcggggtg gtcgctggag ccgcgcgagc
     2401 tgttgccgca ca
//
LOCUS       DASSGX010000529         2632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_95675, whole genome shotgun sequence.
ACCESSION   DASSGX010000529 DASSGX010000000
VERSION     DASSGX010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95675"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1142)
                     /locus_tag="VFI12_09545"
     CDS             complement(<1..1142)
                     /locus_tag="VFI12_09545"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HET7056694.1"
                     /translation="MSSARANTSVFGYIGRRMALVNAAVVLLLIAVVGAGMVGVARYR
                     MIQQADQALKDQANVAALDWGPLLATGQPIPVADRPVDGDGDGHDGDDAHDAEELLDS
                     GDLISFGFDARGTLVANPRGLRTAEFPNTQAVTDALDGEDSFQTIAHHDAPNVRVYTM
                     PIEIDHRVVGAVQIVRNQRDLRSAIRAIELAALAGAAIGALGAVPAGLYLARRAMRPI
                     DRTFRRQQEFIADASHELRTPLSVVRANTELLRRMPDATPAERDAELAVVLDEVDRMA
                     RLVDELLVLARTDAGRLALKTDVVDLSAIVREVVEPMRALATDAGLTMAIDAPDEQLV
                     TGDEDRLAQVLRILVGNAIAYTPRGGTIRVSSFSFPVSSERGPLDKT"
     gene            complement(1129..1797)
                     /locus_tag="VFI12_09550"
     CDS             complement(1129..1797)
                     /locus_tag="VFI12_09550"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7056695.1"
                     /translation="MRILIVEDEERLAGIIARVLTQERFTVDVIHDGEAGLDAALTGA
                     YDAIILDRMLPKRDGASFVRELRAEQIPTPVLMLTALSDLPDRVEGLDAGADDYLGKP
                     FAFEELLARLRALVRRTDKPLAPDRVEVGPVTIDLQRRAVERDGAPVDLSAREFALLE
                     LLARNRGQVLSRDTILERVWGIDAEPQGNVVELYVFYLRRKLGHTAAHPIIRTVRGAG
                     YVLG"
     gene            complement(1902..2300)
                     /locus_tag="VFI12_09555"
     CDS             complement(1902..2300)
                     /locus_tag="VFI12_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056696.1"
                     /translation="MEMRGVNPTLKLAGLVSVALVGLLHLIEAPEYYGEVKYIGALFV
                     LCAIGAAAAVFGIQKDLRAGWMLGAALAGGNFIAYILSRTVGLPQFRENSWSQFLEPM
                     GLLSLAVEAVAFVICLRALRADAEGQVVLA"
BASE COUNT          450 a          915 c          859 g          408 t
ORIGIN      
        1 gttttatcga ggggacctct ctcgctagaa actggaaacg agaaactgga aacccggatc
       61 gttccgccgc gcggtgtata cgcaatcgcg ttgccgacga ggatgcgcag gacttgggcc
      121 aggcggtcct catcgcccgt gaccagttgc tcgtctggcg cgtcgatcgc catggtcagg
      181 ccggcgtccg tcgccagcgc ccgcatcggc tcgaccacct cgcgcacgat agccgagaga
      241 tcgaccacgt ccgtcttgag cgcgagccgg ccggcatcgg tccgggccag caccagcagc
      301 tcatcgacca accgcgccat ccggtccacc tcatcgagca cgacggccag ctccgcatcc
      361 cgctcggccg gcgtcgcatc gggcatccgc cggagcagct ccgtattggc ccggaccacg
      421 gagagtggcg tgcgcagctc gtgcgaggcg tcggcgatga actcctgctg gcgacggaag
      481 gtccggtcga tcggccgcat cgcccgcctg gcgaggtaga ggccggccgg caccgccccg
      541 agcgcgccga tcgccgcgcc ggccagcgcc gccagctcga tcgcgcggat cgcggagcgc
      601 agatcgcgct ggttccgcac gatctggacc gcgccgacga cccggtgatc gatctcgatc
      661 ggcatcgtgt agacgcgcac gtttggcgca tcgtgatgcg caatggtctg gaaactgtcc
      721 tccccgtcga gtgcgtccgt cactgcctgc gtgttgggaa actcggctgt ccgcaacccg
      781 cgtgggttgg cgacgagtgt gccccgggca tcgaacccga aggagatcag gtcgccgctg
      841 tcgagcaact cttccgcgtc gtgcgcgtcg tctccatcgt gcccgtcccc atcgccgtcg
      901 accgggcggt ccgcgaccgg gatcggttgg cccgtcgcca ggagcggacc ccagtcgagc
      961 gcggccacgt tcgcctgatc cttgagcgcc tggtcggcct gctggatcat ccggtagcgg
     1021 gcgacaccga ccatccccgc cccgaccacg gcgatcagga gcaggacgac ggcggcgttg
     1081 accagcgcca tccgccggcc gatgtatccg aagacgctcg tgttcgccct agccgaggac
     1141 atagccggcc cccctgaccg tgcggatgat cggatgcgcg gcggtgtgcc ccagcttgcg
     1201 gcgcaggtag aagacgtaca gctcgacgac gttgccctgc ggctcggcgt cgattcccca
     1261 gacccgctcg aggatcgtgt cccggctcag gacctgtccc cggttgcggg ccagcagctc
     1321 gagcagggcg aattcccggg ccgagagatc gaccggtgcg ccgtcccgct ccacggcgcg
     1381 gcgctggaga tcgatcgtga ccggcccgac ctcgacccga tccggcgcca gcggcttgtc
     1441 cgtgcgccgg accagcgcgc gcagccgggc cagcaactcc tcgaatgcga agggcttgcc
     1501 gagatagtca tccgcgccgg catccagccc ctcgacccgg tcgggcaggt cgctgagcgc
     1561 ggtcagcatc aggacgggcg tcgggatttg ctcggcgcgc agctcgcgga cgaagctcgc
     1621 accgtcccgc ttcggcagca tgcggtcgag gatgatcgcg tcgtaggcgc cggtcagggc
     1681 ggcatcgagg ccggcctcgc catcgtggat gacgtcgacc gtaaatcgct cctgcgtcaa
     1741 cacgcgggcg atgatgccag ccagccgttc ttcgtcttcg acgatcagga tgcgcatgcg
     1801 atgaacctgc gacgtaccgc gctccacggg cagcatacgc cgaagccgcg cgggttgggc
     1861 gcggcttcgg gtgcggtggg aaggggaagg agaatgcgtg ctcaggcgag gaccacctga
     1921 ccctcggcat cggcgcgcag ggcgcggaga cagatgacga acgcgaccgc ctcgacggcg
     1981 agagagagca gccccatcgg ctccaggaac tgggaccacg agttctcgcg gaattgcggt
     2041 aggccaacgg ttcggctcag gatataggca atgaagttgc cgccagccag ggccgcaccg
     2101 agcatccagc cagcgcgcag atccttctgt atgccgaata ccgccgcggc cgcgccgatc
     2161 gcgcagagca cgaacaacgc gccgatgtac ttcacctcac cgtaatactc cggcgcttcg
     2221 atcaggtgca ggagtcccac cagggccacg gacaccaggc cagccagttt cagcgtcgga
     2281 ttaacgcctc tcatttccat gcgatcgact ccatcgctgc gggacagcag cgcagtcggc
     2341 gccgccatcc cgtcatatcg ccggcagccc ccgccaccag cagttcccca cgatgccgga
     2401 ccgaaattag aacgggatta gggccgttgg tcgttggacg tcaggagatg gacacggaaa
     2461 accgaaaacc gaaaacgaaa atccatcgac caatgcccat cgactccgac tgcccatctc
     2521 caccgtcgtc cgccatctgt taacaagttg aacggacacc tgtgctcgtc tatgctgatt
     2581 cggccggtgc tggcggcgtc acctcgggct aggtaggcct atgcgttcct tc
//
LOCUS       DASSGX010000530         5153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_311042, whole genome shotgun sequence.
ACCESSION   DASSGX010000530 DASSGX010000000
VERSION     DASSGX010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5153
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311042"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1407)
                     /locus_tag="VFI12_09560"
     CDS             complement(<1..1407)
                     /locus_tag="VFI12_09560"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET7056697.1"
                     /translation="MIETTSSRPSIGAPMIVEMPKGRQPLLTIVVATAVWTIAAAVVF
                     VLGGYQAIVSINARVALQAIAIVVLAVWSFATIRKPDLRPAGGLIAPVLAAAGAYATS
                     TVLSQRPRLSIEPGLAGLAFVIGFFVLREILSDVWLRRRFVVLLTTLTSAVALLYVVL
                     VAWEWIQWWVTIGRLSFPPLRPSWSSLSFGSPNLVAAFLLLLGPLASVLLGTIAPPRI
                     AWTLSFTILFALILTGARAAWLGLAVGAGFGFVATFVGRGGLSPIRASRPRLMVVSTV
                     AGVMGLLALPAILYRFAQGGEEIRFDLWRSAIAIFSEYPIFGSGPGTWVQLKTIANEP
                     GIPNPIFTHAHSLYFQTAAELGAAGLATSILLAIAVLRLVFRRTSMPAPVARESIAVA
                     VGLVGFAVEAAFDTLTNTPMVCLLVVALVAWVDSEAPGPRPRPGRAVQRIGMAIPVIV
                     IVGLLVTVPQVAQMDAAWA"
     gene            1364..2308
                     /locus_tag="VFI12_09565"
     CDS             1364..2308
                     /locus_tag="VFI12_09565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET7056698.1"
                     /translation="MGAPMEGRELVVSIITPSLNQGRFIEQALASMRAQTCGCVEHIV
                     VDGGSTDETLEILGRAQTAGDLRFISEADGGMYDALNKGLRMANGEILGYLNCDDAYT
                     PWAIETAVRVLEANPDADAVFGDGLTIDESTGRQRLSLLPPFDRDALALSGSLVQPAV
                     FWRRSAYERIGGFDAGLRYVGDLDYWLRLGATARFVRVDEVLAIERHHDAALSRAASN
                     QMTIEEAEMRARHRSVTRTSRLFARARAAAWRRYQWLRFLMRVRDAAPGAGPWSEFTR
                     NGDLQVAPGRVVAMFIPRVGGPYAWDAVRSRRDWFGAG"
     gene            complement(2305..3477)
                     /locus_tag="VFI12_09570"
     CDS             complement(2305..3477)
                     /locus_tag="VFI12_09570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET7056699.1"
                     /translation="MRFVLAVAEHPWPSTSGGQLRTSAIAATLAQLGEVDVISLNMPE
                     PPPLLAPALERYWARRYSQPRRLLDLATGIATAHHVSLNRAIAAGLHIAFADYLTSIH
                     PDTVVLGRPFFGPFIGAARAAGANVIADPDESLVRVSRSVMRSRAPTSLRARAFLDLL
                     SAGRMERRDFRTVNQLWAGSPIEAAALARAADGVPIHIVPNVASRIPDQIAAPWPIRQ
                     VAFVGSFGHPPNEEAAMELMRSIMPAIRASGGPEELVLIGRSPHPRLYRLAKATGGVT
                     ITGAVDDVDVPLRAAGVLVSPIRSGGGTRLKVLDAAAAGVPIVTTAFGVEGLDLVDGE
                     HMLVAETRDAFARAVRRLQTEAGLVERLTWNAAAFVREHHSRSALRQAIAKALELL"
     gene            3540..4784
                     /locus_tag="VFI12_09575"
     CDS             3540..4784
                     /locus_tag="VFI12_09575"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HET7056700.1"
                     /translation="MRTALDVRVPAGQWGGIQQMVEGLARGLSDLDGDDEFLFIGFED
                     AAMWLDPLLGGSCRRVEVGRAHGSSWRRRAYDSVTSRLPGARHLAAVAGQRMGRLSTP
                     IPGSDGFLESLGVDAVHFVTPQAYRTQVPSVYQVMDLLQDHLPYFFSPFHRRYRQVTY
                     RAFSDQAALISTMTNWTRSDIAERLEQPASKIAVVPLPPAVTPETTLPKAPTGLENLA
                     DRFLLYPAQTWPHKNHLALVDAMGFLRDQGSDVDLVCTGRLTEHHPVIARRVEQLGLG
                     PHVRFLGYVEQSELAWLYRNASALVFPSLFEGWGIPIVEAMAWSLPVACSDIPVLDEV
                     AGSAALRFEPRDSTTIAAAIGRITADAALRRTLIAAGRARLEPLTWDRIARTFRALYR
                     RAAGQRPTLEDAAYLMPPTLQL"
BASE COUNT          956 a         1660 c         1669 g          868 t
ORIGIN      
        1 agcccacgct gcatccatct gcgccacttg gggcacggtg accagtagcc ccacgatcac
       61 tattacgggg atggccattc cgattcgctg gacggctcgc cctggccgcg gcctgggacc
      121 gggcgcttca gaatcgaccc acgctacgag cgctaccacg agcaggcaga ccattggcgt
      181 atttgtcagc gtgtcgaagg cggcttccac cgcgaatcca acaagaccta cagctacggc
      241 gatggactcc cgagcgacgg gtgctggcat tgaggtgcga cgaaatacga gtctcagaac
      301 ggcgattgcc aagagaatgc tcgtcgccag gcccgcggca cccaattcag cggctgtttg
      361 gaagtacaag ctgtgggcat gggtaaagat ggggtttggg atgcctggct cgttggcgat
      421 cgtcttcaac tgcacccaag tgcccggccc agagccaaag attggatatt cgctaaagat
      481 cgcgatggcc gaccgccaaa gatcgaagcg gatctcctcc ccgccctgtg cgaagcgata
      541 taggatcgca ggaagagcta gtagacccat cactcctgcc actgtcgaga cgaccatcag
      601 cctgggtctc gatgcgcgga ttggcgagag tccgcctcga cccacgaagg tcgcgacgaa
      661 cccgaacccg gccccaactg ccaatcccaa ccaggcggcg cgcgcgccgg tcaggatgag
      721 cgcgaacaag atcgtgaacg acagcgtcca cgcgatccgg ggaggtgcga tggtgccgag
      781 cagcacgcta gccagtggtc ccaagagcaa aaggaaggcc gcgacgagat tcggactgcc
      841 gaacgacaaa ctcgaccaag aaggtctgag cggcgggaat gacagcctcc cgatcgtaac
      901 ccaccattgg atccactccc acgcgaccaa aaccacatag agaagagcca cggcgcttgt
      961 cagcgtggtc aggaggacga cgaagcgacg gcgaagccat acgtccgaaa gaatttcccg
     1021 caagacgaag aagccaatca cgaaggccag tcccgctagc cccggttcaa tgctcagcct
     1081 cgggcgttgg ctcaacacgg tcgaggtcgc ataggcgcct gcggccgcca gaaccggcgc
     1141 tatcaggccg ccagcagggc ggaggtccgg cttccgaatc gtcgcgaagc tccaaaccgc
     1201 gagcaccacg atggcaatcg cctgcagggc cactcgtgca ttgatggaca cgatcgcctg
     1261 atacccgccg aggacgaaaa cgaccgcggc ggcgatcgtc catacagcgg tggcgacaac
     1321 aatcgtaagg agcggctggc gcccttttgg catctcgaca atcatgggcg cgccgatgga
     1381 agggcgcgag ctcgttgttt cgatcatcac cccgagcctg aaccagggtc ggtttatcga
     1441 gcaggcgctc gcctcaatgc gggcgcagac ctgcggctgc gtcgagcaca tcgttgtgga
     1501 cggcgggtcg acagacgaga ccttggagat tcttggtcgc gcgcagaccg ccggcgacct
     1561 ccgattcatc tccgaggccg acggtggaat gtacgacgca ttaaataagg gccttcggat
     1621 ggcgaacggc gagatcctcg gctacctgaa ctgtgatgac gcctacacac cttgggccat
     1681 cgagacggcg gtccgcgtcc ttgaggcgaa tccggacgcc gacgccgtgt tcggtgacgg
     1741 cctgacgatc gacgagtcga ccgggcgtca gcgcctctcg ctgctccctc cgttcgatcg
     1801 cgatgcgctg gcactgtccg gatctctcgt ccaaccggcg gtcttctggc gccgctcggc
     1861 gtacgagcgg atcggtggct tcgacgctgg cctgcgctat gtcggcgatc tcgactactg
     1921 gcttcgactg ggcgcgactg ctcgattcgt gcgcgttgac gaggtcctcg cgatagaacg
     1981 ccaccacgac gccgcgctga gccgggcggc ttccaatcag atgaccatcg aggaggccga
     2041 gatgcgcgcg cgacaccgca gcgtaacgag gacatcccgc ctgttcgccc gggcccgggc
     2101 ggcggcctgg cgtcgctacc aatggctgcg cttcctcatg cgcgtgcgcg atgcggcacc
     2161 cggcgccggc ccatggagcg agttcacccg aaacggcgac ctgcaagtcg cgcctgggcg
     2221 cgtggtcgcg atgttcattc cacgagtggg tgggccatac gcctgggacg cagtgcgatc
     2281 gcgtcgggac tggttcggcg ccggttagag gagctccagc gccttggcga tggcctgccg
     2341 gagcgccgat cgcgagtggt gctcccgaac gaacgccgcg gcgttccagg ttaggcgctc
     2401 aactaggccc gcttcggtct gcaaccgtcg aaccgcgcgt gcgaacgcat cccgcgtctc
     2461 cgcaacgagc atatgctcgc cgtcgaccag gtccagcccc tcgacgccaa acgcggtcgt
     2521 cacaatcggc accccggccg cagccgcatc aaggactttg agccgcgtcc caccgcccga
     2581 gcggatcggc gacacgagca cgccggctgc acggagcggg acgtcgacat cgtccaccgc
     2641 gccggtgatg gtgacgccac cggtggcttt ggccaggcga tacaagcgcg gatggggact
     2701 gcggccgatc aggacaagct cttcgggccc acccgaagcg cggatcgccg gcataatcga
     2761 gcgcatgagc tccattgcgg cctcctcgtt cggtgggtgc ccgaacgaac cgacgaaggc
     2821 gacctgccga attggccagg gcgctgcgat ctggtctggg attctgctcg cgacgtttgg
     2881 aacgatgtgg atcggtacgc cgtctgcggc ccgtgcgagc gcggcggcct caattggcga
     2941 accagcccaa agctgattta ccgtacggaa atcgcggcgc tccatccgac ccgccgacaa
     3001 caagtcgagg aatgcccgcg cacgcaacga cgttggcgct ctcgaccgca tcacggaacg
     3061 agacactcgg accaggctct cgtcagggtc ggcgatgaca ttcgcgccgg ccgcacgggc
     3121 tgctccgatg aatggaccga aaaacggtct ccccagaacc accgtgtcgg gatggatcga
     3181 cgtgaggtag tcggcgaacg cgatatgcag gcccgctgcg attgcgcggt tgagtgagac
     3241 gtgatgagcg gtcgcaattc cggttgccaa gtccagcaag cgcctcggct gggagtagcg
     3301 ccgcgcccaa taccgttcaa gcgcgggagc caggagcggc ggcggctccg gcatgttcaa
     3361 cgagatcacg tcgacctcgc cgagttgagc gagcgtcgca gcaatggcgc tcgttcggag
     3421 ctgccctccg ctggtgctgg gccagggatg ctctgcgacg gcgaggacga acctcatggg
     3481 cgatgcggac atcagcacaa gcctacggcg cgaggtcgtg ccgctatcgt ggggggccga
     3541 tgcgtaccgc gctcgacgtt cgcgtgccgg ccggccagtg gggcggcatc cagcagatgg
     3601 ttgagggcct ggctcgcggc ctcagcgacc tcgatgggga cgacgagttc ctcttcattg
     3661 ggttcgagga tgcggcaatg tggctcgacc ctctactcgg cgggtcatgt cgccgcgtgg
     3721 aggttggccg agcacacggg tcgtcatggc gtcgtcgcgc ctacgacagc gttacctcgc
     3781 gattacccgg tgcccggcac ctggctgctg tagcggggca acgaatgggg agactgtcca
     3841 cgccgatccc tggctcggac ggcttcttgg aatcgcttgg tgtggacgcc gtccatttcg
     3901 tcacgccaca ggcgtatcga acgcaggtcc cgtccgtgta ccaagtgatg gaccttttgc
     3961 aggaccacct gccgtacttc ttctcgccat ttcaccgtcg atatcgacag gtgacatatc
     4021 gcgccttctc ggatcaggcg gcactcatct cgacgatgac gaattggacg cgttcggaca
     4081 tcgcggagcg gctggagcag cccgcgtcga aaatcgccgt cgttccgctg ccgccggcgg
     4141 tgacgccgga gacaacattg cccaaagcgc caactgggct tgagaacttg gccgatcgat
     4201 tcctcctgta tccagcccag acttggccgc acaagaacca cctggcactg gtcgacgcaa
     4261 tgggtttctt gcgagatcag ggctcggacg tcgaccttgt gtgcaccggc cgactcaccg
     4321 aacaccatcc ggtcatagcg cggcgcgtgg agcaactcgg gctcggccca catgtgcgtt
     4381 tcctcggcta cgtcgagcaa tcggaactcg catggctata ccggaatgct tccgcgctgg
     4441 tttttcccag cttgtttgag ggctggggaa tcccaatcgt cgaggcgatg gcgtggagcc
     4501 ttccggtcgc ttgctccgac atcccggtcc tcgacgaggt cgcaggcagc gcagcgttgc
     4561 gttttgagcc cagagattcc acaacgatcg ctgcggccat tgggcgcatc accgcggacg
     4621 cggcccttcg ccgaacactc atcgcggctg ggcgagcacg cctcgagcca ttgacttggg
     4681 atcgcatagc cagaacgttc cgggcactct atcggcgagc cgcgggtcaa cggccaacgc
     4741 tcgaggacgc cgcctatctc atgccgccca cactccagct atgagcaagg tcgaggtgct
     4801 gggcgtaccc gttgacgccc agcgctatga ggcagccgcg accgcactac tgcaagcagc
     4861 gagatccgga gaccataggc gtgcgcattt cgcgacggtc cacagcgtta tcgcggcggc
     4921 ggactcgaag accgtgctcg atgccttccg atcggcatgg atggtttgca ccgacgggat
     4981 gccgctcgta tgggtcgctc gccgacgcgg tgccgtggcg gagcgcgttt gcgggccgga
     5041 catgatgctc accatctgtg accgcggccg tgtttcgggg ctgcgtcact actttctcgg
     5101 tggcgcgccg ggcactgccg atctgctcgc gtcggccatg aaggagcgat tcc
//
LOCUS       DASSGX010000531         3479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_748133, whole genome shotgun sequence.
ACCESSION   DASSGX010000531 DASSGX010000000
VERSION     DASSGX010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748133"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VFI12_09580"
     CDS             <1..210
                     /locus_tag="VFI12_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056701.1"
                     /translation="RWTNAGRDDDAVLLRAAELAAWFSQSRASGRVEVDITPRRFVRK
                     IKGAGPGMVTYRNERTVSVAPVGPD"
     gene            complement(207..980)
                     /gene="tatC"
                     /locus_tag="VFI12_09585"
     CDS             complement(207..980)
                     /gene="tatC"
                     /locus_tag="VFI12_09585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HET7056702.1"
                     /translation="MRADDGSEEIFEEMTLGEHLIELRTRIVRACLSIGAAFIIGVIL
                     AKPLLDRIRHDSLATNGFDIRSPTDPLTIYFKVALYIAVALAFPLILYQLLAFVSPGL
                     TRKEKRVVYTALPFVAILAILGGTYGLLVAAPRALYFLSRFMSSVFQWEPDADTIITF
                     YLTLMLGLALAFQIPVVMFILAKIGLVTPKKMASIRKYAFIVILILAAIITPSTDPFN
                     MSIVAAPLYVLYEFGIIISRIFAKSPMISVPPPTATDAD"
     gene            complement(1180..1416)
                     /gene="acpP"
                     /locus_tag="VFI12_09590"
     CDS             complement(1180..1416)
                     /gene="acpP"
                     /locus_tag="VFI12_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET7056703.1"
                     /translation="MASVYERVRSIVSERLGVDEDKVTMDAEFIGDLNADSLDLVEVI
                     MAMEQEFDTEIKDEDAENIRTVSDAVKFIEEHAS"
     gene            complement(1589..2059)
                     /gene="nusB"
                     /locus_tag="VFI12_09595"
     CDS             complement(1589..2059)
                     /gene="nusB"
                     /locus_tag="VFI12_09595"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HET7056704.1"
                     /translation="MSDSTHHASRQRKQRNTRRHQARILAMQIMYEADMTGHSTSEIL
                     IRTRTQGGTPDETLEYTSALLIGVRARVDAIDAEIEAVAPQYPVNDLSPIDRAILEIA
                     LFEILFGEDVPPRAAVNEAVGIAQEYGGEASARFVNGVLGSVIDRHFPDSSRRR"
     gene            complement(2059..2499)
                     /locus_tag="VFI12_09600"
     CDS             complement(2059..2499)
                     /locus_tag="VFI12_09600"
                     /inference="COORDINATES: protein motif:HMM:NF015721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET7056705.1"
                     /translation="MSAAEPLTHHVLAEISENGMHGTISISPNVLAEIIELASAGTEG
                     LVKFASPGRNRGKTLPIGDDAQHELKGGDWFQRSGIRVRLDNATVDAELSVDVQHGAH
                     VPTMADELKRRISEAVDHMLGLRTGIIAIHVRSISPPASGTGEH"
     gene            complement(2496..>3479)
                     /locus_tag="VFI12_09605"
     CDS             complement(2496..>3479)
                     /locus_tag="VFI12_09605"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922565.1"
                     /note="an AccC homodimer forms the biotin carboxylase
                     subunit of the acetyl CoA carboxylase, an enzyme that
                     catalyzes the formation of malonyl-CoA, which in turn
                     controls the rate of fatty acid metabolism; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HET7056706.1"
                     /translation="GIVRNLAEAKASANKVGYPVLIKAAAGGGGRGMRVANSESELIR
                     QLPMAQTEAESAFGNGDVYIERYLDKPRHVEVQVLADAHGHVYAIGDRDCSLQRRHQK
                     IIEEAPAPFLAKRIRDNILKAAAKGAKAAGYRSAGTLEFLVDSEGRFYFMEMNTRIQV
                     EHPITEMVSNIDLVTWQLRIAMGEQLRLEERDFEPRGHAIECRITAEDPARDFAPSVG
                     TVSTYVAPGGPGVRVDSHLFCGYQVPPFYDSLLAKVAVWGKDRAEAIERMERALRETI
                     IDGVINVAPLHRMIMMDEPFRKGEIHTGYVADFLKRSFDLVADAQQDDIGP"
BASE COUNT          631 a         1071 c         1128 g          649 t
ORIGIN      
        1 cgctggacga atgccggccg ggacgacgac gcggtgctgc tccgtgcggc tgagctggcg
       61 gcctggttca gccagtcccg cgcgagcggc cgggtagagg tggacataac gccgcgccgc
      121 ttcgtgcgca aaatcaaggg cgccggtccc ggcatggtca cctatcggaa tgagcggacg
      181 gtatcggttg cgccggtcgg cccggactaa tccgcgtccg tcgcggtcgg tggcggaacg
      241 ctgatcatcg gtgacttggc gaagatgcgg gagataataa tcccgaattc gtagaggacg
      301 taaagtggcg cggccacgat cgacatgttg aacggatcag tactcggcgt gatgatggcg
      361 gcgaggatca ggatcacgat aaacgcatat ttcctgatcg aggccatctt cttcggcgtg
      421 acgaggccga tcttggccag gatgaacatc acaaccggga tctggaacgc gagcgccaaa
      481 ccgagcatca acgtcaggta gaaggtgatg atcgtgtccg catccggttc ccattggaag
      541 acgctggaca tgaaccggct caggaagtaa agcgcccggg gagcggccac cagcaggccg
      601 tacgtgcctc cgagtatcgc caggatcgcc acgaacggca gggcggtgta gaccacccgt
      661 ttttccttgc gcgtcaagcc gggagagacg aacgccagga gctgatacag gatcagcgga
      721 aaggcgagcg cgacggcgat gtagagcgcg accttgaagt agatcgtcag cgggtcggtg
      781 ggggagcgga tgtcgaatcc gttcgtcgca agcgagtcgt gccggatgcg atccagcagc
      841 ggcttggcga ggataacgcc gatgatgaac gctgcgccga ttgagaggca ggcccggacg
      901 atcctggtgc ggagctcaat caagtgctcc ccgagggtca tctcctcgaa tatttcttcg
      961 gaaccgtcgt ccgcgcgcat ccagtcggga cggctgggtc gccggagccg gggcggcttc
     1021 ggcatgcggg gaactggcag tttagcgagt cgggacatcg atctacccgg gtatgagccc
     1081 tgaaccaaaa gggcgggcca cacagcggcc cgcccttccg caatcgtcag agaccagtat
     1141 tgcggccctg agagccgctg acaagcactc ggctgagaat cagctcgcgt gctcctcaat
     1201 gaacttcact gcatcggaca cagttcggat gttctccgcg tcctcgtcct tgatctcggt
     1261 atcgaactcc tgctccatgg ccatgatgac ttcgaccagg tcaagagagt ctgcgttgag
     1321 atcgccgata aactcagcgt ccatcgtcac cttgtcttca tcaacgccaa ggcgctccga
     1381 gacaatgctg cgtacacgct catataccga cgccaacgtt cccacctcca gcgaaagcca
     1441 acctgtccat caggagcaac cgaaccagtg gatgctccga gcgcggtgac tgtgtgatcg
     1501 aaacggtgaa cgccaaccag tcatagacac tcgcgccgga ttctacacga cggctgatac
     1561 agcggccacc gagaagccac cggccatgtc agcgccggcg gctggaatcg gggaagtgac
     1621 ggtcaatgac gctgccgaga acgccgttca caaaccgggc gctggcctcg ccgccgtatt
     1681 cctgggcgat gccgaccgcc tcgttgacgg ccgcccgggg tggcacgtcc tcgccgaaca
     1741 ggatctcgaa cagcgcgatc tcgaggatgg cccggtcgat cggggacaga tcattgaccg
     1801 ggtattgcgg cgcgacagct tcaatctcgg cgtcgatcgc gtccactcgg gcgcgcacgc
     1861 cgatcagcaa tgccgaggtg tactccagcg tctcatccgg cgtgccgccc tgagtccgcg
     1921 tacggatcag gatctcgctt gtcgaatggc cggtcatatc ggcttcgtac attatctgca
     1981 tggccaggat gcgggcctgg tggcgccggg tattgcgctg ctttcgctgc cggctggcgt
     2041 ggtgcgtgga gtcgctcatc agtgctcccc ggttccgctt gccggcggcg agatcgaccg
     2101 cacgtggatc gcaatgatgc ccgtccgcag cccgagcatg tggtcgaccg cctcgctgat
     2161 tcgccgcttc agctcgtcgg ccatcgttgg cacatgtgcg ccgtgctgga cgtcgacgga
     2221 gagctccgcg tcgactgtgg cgttatcgag gcggacacga atgccgcttc gttgaaacca
     2281 gtcgccaccc ttgagctcgt gttgcgcgtc gtcgccgatc ggcaatgtct tgccgcgatt
     2341 ccgacccggt gaggcgaatt tgaccaaccc ctcggtgccg gccgaagcga gctcgatgat
     2401 ctcggccagg acgttgggcg agatggagat ggtgccgtgc atcccatttt cggaaatctc
     2461 ggccaggaca tgatgcgtga gtggctccgc ggcactcatg gtccgatgtc gtcttgctgg
     2521 gcgtccgcga cgaggtcgaa gctgcgcttc aggaagtcgg cgacgtaccc agtatggatt
     2581 tcgcccttgc gaaacggctc gtccatcatg atcatccggt ggagcggcgc gacgttgatg
     2641 acaccgtcga tgatcgtctc gcgaagggcc cgttccattc gttcgatcgc ctcggcgcgg
     2701 tccttgcccc agaccgcgac cttcgccagc agcgagtcat agaacggcgg aacctggtaa
     2761 ccgcagaaca gatgggagtc gacgcgaacg ccgggtccgc cgggcgccac gtaggtcgag
     2821 accgtgccga cgcttggcgc gaagtcgcgt gccgggtcct cggccgtgat ccggcactcg
     2881 atcgcgtggc cgcgcggttc gaaatcgcgc tcttccaggc gaagctgctc gcccatggcg
     2941 atccggagct gccaggtgac caggtcgata ttcgagacca tctccgtgat cgggtgctct
     3001 acctggattc gggtgttcat ctccatgaag tagaagcggc cctcgctgtc gaccaggaac
     3061 tcgagggtgc cagcgctgcg gtatccggcc gctttggcgc ccttggctgc tgctttcagg
     3121 atgttgtccc ggattcgctt ggccaggaat ggcgccggcg cctcctcgat gatcttttgg
     3181 tggcggcgct gaagcgagca gtcgcggtcg ccgatcgcgt aaacgtgccc gtgggcatcg
     3241 gcgagcacct ggacttcgac atggcgcggc ttgtcgagat agcgctcgat gtagacatcg
     3301 ccattgccaa aggccgattc cgcctcggtc tgggccatcg gcaattgccg gatcaactcg
     3361 ctctcgctgt tggcgactcg catcccgcgc ccgccgccgc ccgccgccgc cttgatcagg
     3421 accggatagc cgaccttgtt ggccgatgcc tttgcctcgg ccagattgcg gacgatgcc
//
LOCUS       DASSGX010000532         2093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_968005, whole genome shotgun sequence.
ACCESSION   DASSGX010000532 DASSGX010000000
VERSION     DASSGX010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968005"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="VFI12_09610"
     CDS             <1..329
                     /locus_tag="VFI12_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056707.1"
                     /translation="LMEILLADTHPDFVDLEPDLYWIAEAGIDPVEQLLEMHPRVRMI
                     HAKDRAADGAIANVGSGIFDWDAIIAASKRVMATYLIVERDDPTDLMSDLSDSYRFLE
                     TKLASV"
     gene            complement(340..633)
                     /locus_tag="VFI12_09615"
     CDS             complement(340..633)
                     /locus_tag="VFI12_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056708.1"
                     /translation="MVTQPPTDPDMRPDPKTYPPAEPDPMDPNFPQFPDEPGLPEIGD
                     PTTPQRPDIPGEDDPYGFPGPSTIPDIGDPTDKDDTTAYPMAFPADGTNPNAG"
     gene            855..1601
                     /locus_tag="VFI12_09620"
     CDS             855..1601
                     /locus_tag="VFI12_09620"
                     /inference="COORDINATES: protein motif:HMM:NF018757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HET7056709.1"
                     /translation="MRVLLAGESWVVHSIHQKGFDSFTTTAYAEGHHWLSAALKTGGA
                     ELDYLPSHLANDQFPDTAEELARYDVVMLSDIGANTLLLPSDTFMKSVSRPNRLEAIR
                     QYVENGGGFVMVGGYLTFQGIDAKAQYASTPVEDALPVTLMYGDDRVEAPQGVTPTVD
                     EAEHPIVAGLPASWPNLLGYNLLEPREQATVVASVGTDPLIAVWEYGQGRSVAFASDC
                     GPHWAPPAFVEWDGYARLWQQLVGWAAGGK"
BASE COUNT          363 a          679 c          690 g          361 t
ORIGIN      
        1 ccctgatgga gatcctgctc gcggacacgc accccgactt cgtcgacctc gaacccgacc
       61 tctactggat cgccgaagcc ggcatcgacc cggtcgagca gctcctcgag atgcacccgc
      121 gcgtccgcat gatccacgcc aaggaccgcg ccgccgatgg ggcgatcgcg aacgtcggca
      181 gtggcatctt cgactgggac gcgatcatcg ccgcgtcgaa gcgggtgatg gccacatacc
      241 tgatcgtcga acgcgacgac ccaaccgacc tgatgtccga cctctccgac agctaccgct
      301 tcctcgagac aaagctcgcg tcggtctgat cgaattcagt cagcccgcgt tcgggttagt
      361 gccgtcggcc gggaaggcca tcggataggc ggtagtgtca tccttgtcgg tcgggtcgcc
      421 gatatccggg atagtcgacg gcccggggaa accataggga tcatcctcac ccgggatgtc
      481 cggtcgctgg ggcgtcgtcg gatcgccgat ctccggcagg cccggctcat ccggaaactg
      541 cggaaagttc gggtccatcg gatccggctc agccggcggg taggtcttcg ggtccggccg
      601 catgtccgga tcagtcggcg gttgcgtcac catggctgcg ctcctcgcgt tggttcgagt
      661 acccgacctt cggatgtgcg ttgttgctgg cttcccctgc tccgggtcgg accgtcagtg
      721 tagggatggc ggcagtagcg tcgccggccg ccggactaca gagccggtcg tgagcgcggc
      781 ccgggagaaa tgacgatact accggctgcg gttggccttc ccggcgggag gcggttcagc
      841 gtgagggggt cccgatgcgg gtattgctgg ccggtgaatc gtgggtcgtc cactccattc
      901 accagaaggg cttcgattcg ttcacgacca cggcctacgc cgaggggcac cactggcttt
      961 ccgccgcgct caagaccggc ggagccgagc tcgactactt gcccagccac ctcgccaacg
     1021 accagtttcc cgataccgcg gaggagttgg cgcggtacga cgtggtcatg ctcagtgata
     1081 tcggcgcgaa cacgctcctc ctcccatccg acaccttcat gaagtcggtt tcccgcccaa
     1141 atcggctgga ggcgatccgg cagtacgtcg agaacggcgg cggcttcgtg atggtcggcg
     1201 gctacctgac ctttcagggc atcgacgcca aggcccaata cgccagcacg ccggtcgagg
     1261 acgccctgcc ggtcacgctc atgtacggcg acgaccgggt cgaggcgccg caaggggtca
     1321 cgccgacggt ggacgaggcc gagcatccga tcgtggccgg attgccggcg tcatggccaa
     1381 acctgctcgg ctacaacctg ctcgagcccc gggaacaagc caccgtcgtc gcctcagtcg
     1441 ggaccgatcc gctcatagcc gtttgggagt acggccaagg ccgctcggtc gccttcgcca
     1501 gcgattgcgg accgcactgg gcgccgccgg ccttcgtcga gtgggacggc tacgcgcgac
     1561 tctggcaaca gctcgtcggc tgggctgccg gagggaaata gcgcgccggc ggccagaccc
     1621 gcgccgcgtc gtccgactcg agcgaatcat gcccgatcgc ggcggtcagg tcggtccgcg
     1681 accagagatt gcggctggcc ttccgcccga gcggtacatc cgggttcgaa ccgggctgct
     1741 cgaaaacgag aggtgcgcca agcgggggga tgtatcgcgc cagcgcgctg acgccggatg
     1801 tcaatctgcg tgaaaaatgg aagcgaacca tcgtcgccct ccggtgttca tcatttaggt
     1861 gcacatcggt gggcaaacag ttgggggagc ggcaacgaat cccctgctca ctttcgacag
     1921 taccacgcat ccgtgagctc ttcatgaacg agacgtacac aataggtccc gcctccgggc
     1981 atggttgccg gaggtgcatt cgcggccggg cggaatgccg aattgcgttc aacggtcgac
     2041 atgagagaat gacgcgacgc tgtcagacca gagtgagatc agtgaagggt tcc
//
LOCUS       DASSGX010000533         6624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1512640, whole genome shotgun sequence.
ACCESSION   DASSGX010000533 DASSGX010000000
VERSION     DASSGX010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6624
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1512640"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..1226)
                     /locus_tag="VFI12_09625"
     CDS             complement(66..1226)
                     /locus_tag="VFI12_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056710.1"
                     /translation="MPDRVTAELALDHFWDALVDDASANVDGLDPLDTTSLRRLHEMA
                     STPVPVRSSARVQRTIRDEIAARANGQDLSSDQPSSLERIGARSATAVDQPASPPRPI
                     AKLPATRGARRWASILATAALLLLTLGASIQILDLNPLKRDDRGPAIPAPTAQPGVSE
                     QTLLDVVLTPEESGYDGQFGSGLAYHAVPPGSHDVWPTSNGQATEARAFYIVNGTLRV
                     KGDHPLRVVRANTDGAIEQVPADTWFTLNPGDAFLRTPDTNLELENPGSESSYVLNWT
                     LGGRLGNDGYAGYQPPDWQHINATWGYDGTRPDPPARFTLRHVTLEKGATLSAPNGAY
                     FQFYLNPARDSRAMLAVDSDYSITNRGAASADLFVFAMEPAHPDGGGTPSST"
     gene            complement(1211..1831)
                     /locus_tag="VFI12_09630"
     CDS             complement(1211..1831)
                     /locus_tag="VFI12_09630"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7056711.1"
                     /translation="MRTSGPQIETREQPAPARSDALLVAAAQGDITAFDSLYGRYLDA
                     VLRFCFYRLGDWEQAEDATSEVFVRILSNLAHFQPGSHDGGFRCWLFTIARHVVTDRH
                     RDRTRHPVDPLDAAINVRDVMPTPEEAAIATDDHQFIRALMGQLKPDQRDLLELRLAG
                     LKNAEIARILGRSHEAIRKEQSRIVKSLRILVGQQAEQGGAPCPTV"
     gene            complement(1959..2459)
                     /locus_tag="VFI12_09635"
     CDS             complement(1959..2459)
                     /locus_tag="VFI12_09635"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HET7056712.1"
                     /translation="MLVSLMVKQMNQQAIGELARRAAVVIMVAVGLGAAMMANAAPGN
                     GRGTPSPSPAASPSPVASPSPISLAGHGSATVSHDVTIALTDEGFVPNYIQSTSGHGL
                     NVTLVNTGTRRHGFTIDQLDIDVMLDPGQTKTVVIHPSAGEGDFTYVSTAPGDDGMTG
                     TLTFYI"
     gene            complement(2504..4231)
                     /locus_tag="VFI12_09640"
     CDS             complement(2504..4231)
                     /locus_tag="VFI12_09640"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HET7056713.1"
                     /translation="MRNRTLIAFIAIMALLIPAFGLLAPSSPSPANAQGDQTLPDNPD
                     FPGVATPGGTIPNNPKIQLVKVAGGLADPINVASANDGSGRIFVVERIGRVRIVDKDG
                     NLLPDPFLDIQSLVKTDFLEQGLLGLAFSPDYKTNGLFYVYYSDYQTNGDVVLSEYKV
                     SSDDPNKADPKSARVLFTQDKPYVNHNGGTLRFGPDKMLYISIGDGGLAGDPYDNAQK
                     IDVLLGKVLRIDPNARDAGAYGIPKDNPFANTGVVLPSDQASNMAQDGSYHPDARREI
                     CNWGLRNPWQYNFDPKTGELYLDDVGQNAWEEVDVFPANQPCGWNLGWDHNEGASCYP
                     PGSKCFQAGALPVATYNHNSGDCSITGIGVYRGTDSANLDGIYFNSDFCSGKIYGLQM
                     DGNGAWQYQVLLQTGLSVTGSGQDENGELYVAACSCKYSRDYNALANPGGTVWRIVSA
                     DKVPQGAELAPSPSAVAATPAGGASEAATPGASPVAGGQTIDLVDIAFQPNELTIPAG
                     QDVTITLDNKGNLPHSFDIDALGIDSGEIQPGSSGTVTINAKAGDYQFYCKVPGHKEA
                     GMVGTLHVK"
     gene            4707..5405
                     /locus_tag="VFI12_09645"
     CDS             4707..5405
                     /locus_tag="VFI12_09645"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7056714.1"
                     /translation="MSMTFLVAEVSPDTAHAIEFSAQMSWPGCRVITACDGDEALARF
                     ADEHVDAVLLDAHLNQPDGFDLCRCIRAVSKVPILMLSTDNAVVDKVRAFDCGADDYV
                     VKPFDHLELMARLRALLRRAEMGSPAPAREYACGDLAIDFQWRRVRCHGQEVRLTSTE
                     FRLLEVLVRNAGTTMPHHVLLDRVWGSEWAGRPDYLKVFVRRLRQKLDDDAGRPRYIE
                     TVWGTGYRFLPYSA"
     gene            complement(5459..6463)
                     /locus_tag="VFI12_09650"
     CDS             complement(5459..6463)
                     /locus_tag="VFI12_09650"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7056715.1"
                     /translation="MVAQAPVAPNPRGARAVSLIQQQGALIALLIILAFGLIRYGENF
                     RSSFNIWEMLRNNSYYGLIALGMTFVIMAGGIDLSVGAVAALTAVWAARLSEHGIWVG
                     TLGAVAMGLGIGIINGGIIARFKVQPFIVTLAMLLACRGFALNLAHNANVSVDFASGY
                     IDIGQKNIGRVPLPVVITVIAYIIGSIVLNFTRFGRHVLAVGGSEEAARLAGLPVART
                     TVAIYAISGACAGLAGAILAALAYTGSPNFAIGWELSAIAAVVVGGTLLTGGQGSVWT
                     SLVGVLLLGLIFNELNFERAKGTFELTQYWEEIIRGAFLLIVVLLQARLARQQRLIRR
                     "
BASE COUNT         1166 a         1999 c         2216 g         1243 t
ORIGIN      
        1 gcggtgcccg gttcccttga gcggccggcc tgccgggtac ctcggcggcc ggccgaatca
       61 ggcgatcagg tggacgacgg agtgcctccg ccgtccgggt gagccggctc cattgcaaag
      121 acgaacaaat ctgccgaagc agcgccgcga ttcgtgattg aatagtctga atccaccgcg
      181 agcatcgccc ggctatctcg tgccgggttg aggtagaact ggaaatacgc tccgtttggg
      241 gcggacaggg tcgcaccttt ctcaagcgtg acgtgacgta acgtgaaccg tgccggaggg
      301 tcaggtcgtg tgccgtcata gccccacgtg gcgttgatgt gctgccaatc cgggggttga
      361 tatccagcgt atccatcgtt cccgagacgg ccaccaagag tccagttgag aacgtagctg
      421 gactcgcttc cggggttctc caactcgaga ttggtatccg gagttcgcaa gaatgcatcc
      481 cccgggttca acgtgaacca tgtgtccgcc ggcacctgct cgattgctcc atcagtgttc
      541 gcacgaacca cgcgaagcgg gtgatcgcct ttgacgcgta acgtgccgtt cactatgtag
      601 aaggcacgag cctcggttgc ttgaccgttc gaggtcggcc aaacgtcatg cgagcctggc
      661 ggcaccgcgt gataggcgag tccagagcca aactgaccgt cgtaccctga ttcctccggc
      721 gtcagtacga catcgaggag cgtctgctcc gaaacgcccg gttgggcggt tggggctggt
      781 atcgccggtc ctctgtcgtc gcgcttgagc gggttcaggt ccaggatctg gattgacgcc
      841 ccgagcgtga gtagcagtag ggcggcagtc gccaggatgg aggcccaccg gcgcgccccg
      901 cgggtcgccg gcagtttggc gatggggcgc ggcggcgatg ccggctgatc aaccgcagtg
      961 gccgagcgcg ccccaatgcg ctccagcgac gacggctgat cggaagacag gtcctggccg
     1021 ttcgcgcggg cggcgatttc atcccggatc gtgcgttgca cccgggcgct ggagcgcacc
     1081 gggaccggag tagacgccat ctcgtggaga cgtcgcagcg acgtggtgtc cagtggatcg
     1141 aggccatcga cattcgcgct cgcatcgtcg accagcgcat cccagaagtg atccagggcc
     1201 agttcggcgg ttacacggtc gggcatggag cacccccttg ttccgcctgc tgaccgacga
     1261 ggatgcggag cgatttgacg atgcgggatt gctccttgcg aatggcctcg tggctgcggc
     1321 cgaggatgcg cgcgatctcg gcattcttga ggccggccaa tcgcaattcc agcaggtcgc
     1381 gctgatccgg cttgagttgg cccatcagcg cgcggatgaa ttgatggtcg tcagttgcga
     1441 ttgctgcctc ttccggtgtc ggcatgacgt cgcgcacgtt gatggcggcg tcgagtggat
     1501 caaccgggtg gcgggttcgg tcgcggtggc gatcggtcac gacgtggcga gcgatggtga
     1561 atagccagca ccggaatccg ccatcatggc tgccaggctg aaaatgggcg aggttggaca
     1621 ggatgcgcac gaagacctca ctggtcgcgt cctccgcctg ctcccaatcg ccgaggcggt
     1681 agaagcagaa tcggaggaca gcgtcgagat atcgaccgta gagggagtca aaggcagtga
     1741 tgtcgccttg agcggcggcg acgagcaacg cgtctgagcg cgccggggcc ggctgctcac
     1801 gtgtctcgat ttggggaccc gatgtcctca tccccgctct ccctgatgcc cgacgtgtta
     1861 cctaggagaa acgcgcggcc gggaattatg tgaccaggaa aaggagttgg cttcaggata
     1921 tgccctgacg tcaaatcggg acagtgatct gttggggatc agatgtagaa ggtgagggtg
     1981 ccggtcatgc cgtcgtcgcc tggcgcagtg ctgacgtagg tgaagtcgcc ttcgccggcg
     2041 gagggatgga tcacgaccgt cttggtctgg ccggggtcga gcatgacatc gatatcgagc
     2101 tggtcgatcg tgaacccgtg cctgcgggtg cccgtgttga ccagggtcac gttcaggccg
     2161 tgaccgctgg tgctctggat gtagttcggg acaaatccct cgtccgtgag cgcgatcgtg
     2221 acgtcgtggc tgacggttgc cgagccatgg cccgcgagcg aaatgggact cggggatgcg
     2281 acgggggagg gactggctgc cggcgagggg ctcggtgtgc cgcggccatt acctggggcg
     2341 gcattggcca tcatggcggc gccgagcccg accgcgacca tgatcacgac cgcggcgcgc
     2401 ctcgcaagtt caccgatcgc ttgttgattc atttgcttga ccatgagcga gacgagcact
     2461 gcccgggcat ccacttgccc gggcagtgcg caatgaattg ccgttacttc acgtgcaggg
     2521 tgccgaccat cccggcctcc ttgtgcccgg gaaccttgca atagaactgg taatcgccgg
     2581 ctttggcgtt gatcgtgacc gttccggagc tgcccggttg aatctccccg gaatcgatgc
     2641 cgagtgcgtc gatgtcgaac gaatgcggca ggttcccttt attgtccagg gtgatcgtta
     2701 cgtcctggcc ggccgggatc gtcagctcat tgggctggaa ggcgatgtcg accaggtcga
     2761 tcgtctgtcc gccagcgacc gggctcgcgc cgggagtggc cgcctcggat gcgccgccag
     2821 cgggcgtggc ggccacagct gacggcgacg gggcgagctc cgcgccctgc ggtaccttgt
     2881 cggcgctgac gatccgccag actgtgccgc cgggattggc gagcgcgttg taatcgcgcg
     2941 agtatttgca ggagcaggcg gccacgtaca gctcaccatt ttcgtcctgc ccggatccgg
     3001 tgacgctcag gccggtttgg agcagcacct gatattgcca agccccgttg ccgtccatct
     3061 gcaatccgta gatcttcccg gagcagaagt ccgaattgaa gtagataccg tccaggttcg
     3121 cggagtccgt gccgcgatag acgccgatgc cggtgatcga gcagtcgcca ctgttgtggt
     3181 tgtaggttgc aaccgggagc gcaccggcct ggaagcactt ggaacccggg ggatagcagg
     3241 acgcgccctc gttgtgatcc cagcccaggt tccagccaca cggctggttg gccgggaaga
     3301 cgtcgacttc ctcccacgcg ttctggccca cgtcatcgag gtagagctcg ccggtcttcg
     3361 gatcgaagtt gtactgccat gggttgcgca ggccccagtt gcagatctcg cgccgggcat
     3421 ccgggtgata cgagccgtcc tgcgccatgt tgctggcctg atcggagggc agcacgacgc
     3481 cggtgttcgc gaacgggttg tctttgggga tgccgtaggc gccagcatcg cgcgcgttcg
     3541 ggtcgatccg caggaccttc ccaagcagga catcgatctt ctgcgcgttg tcgtacgggt
     3601 cgccggccag gccgccgtcg ccgatcgaga tgtagagcat cttgtctggt ccgaatcgga
     3661 gcgtgccgcc gttgtggttc acgtacggct tgtcctgggt aaagagcacg cgggcgctct
     3721 tgggatcggc cttgttcggg tcatccgatg agaccttgta ctcgctgagg actacgtcgc
     3781 cgttggtctg ataatcgctg taatagacgt aaaacaaccc gttggtcttg taatcgggcg
     3841 agaaggcgag gccgagcagg ccctgctcga ggaagtcggt cttgacgaga ctctggatgt
     3901 cgaggaacgg gtccgggagg aggttgccgt ccttgtcgac gatccgcacc cggccgatcc
     3961 gctcaaccac gaagatgcgg ccgctgccat cattggcgct ggcaacgttg atcggatcag
     4021 ccaatccgcc ggcgaccttc accagctgga tcttgggatt gttggggatt gtgccaccgg
     4081 gcgtcgccac acccgggaaa tcgggattat cgggcagtgt ctgatcccct tgcgcgttcg
     4141 caggggaggg gctgctcgga gccagcaggc caaatgccgg aatcagcaac gccatgatgg
     4201 caatgaaagc gatcagggtt cggtttctca tgcttgttcg tcctttcctg tcctggatca
     4261 cgcacgacgt cgcgtgatgc cacgtcgact ctggacgcac gacagagcat cgacagccgg
     4321 ccggaccata gcgttatggc agggcgtgct tcttgtcggt taggacaggc ccttgccgtg
     4381 cgaggcaatt gccttccacc ctaggcagat aaggtcaaca aggccgtatt gattggtgaa
     4441 cgaacccggg aacgtttggt gaacggcggt gaacggtctc tccgtcgatt gcgtgggcga
     4501 gaggtagtgt cagcacagcc gctccgcggc tttcgcccgt aatctcgtgg caatatgcgc
     4561 tttgccgggc cgaaatgtgc ctccggctcg tggtgaagcc gtatcacggg accaccacaa
     4621 cgggttgtag ccattgacac ctcatgccca tggcgaggct ggctcaccag tgatcgttgc
     4681 cgcgccgtgg ttgagggaga agccagatgt cgatgacgtt tctggtcgct gaagtatccc
     4741 cggacaccgc ccatgcgatt gagttcagcg cgcaaatgtc ctggccggga tgtcgcgtga
     4801 tcacggcgtg cgacggggat gaggcactcg cgcgattcgc cgacgagcat gtcgacgccg
     4861 tgttgctcga tgctcacctt aatcagccgg atgggttcga tctttgccgc tgtatccgcg
     4921 cggtatccaa ggtgccaatc ctgatgctgt cgactgataa cgcagttgtc gacaaggttc
     4981 gcgcattcga ctgcggcgcc gatgactacg tggtgaagcc gttcgatcac ctcgagctca
     5041 tggcccgtct gcgagcgttg ttgcggcggg cggagatggg aagcccagcc ccggccagag
     5101 agtatgcctg cggcgacctc gccatcgact tccaatggcg cagggttcgc tgtcacgggc
     5161 aggaagtgcg gttgacctcg acggaattcc gcctgcttga ggtattggtc aggaatgccg
     5221 gcacgaccat gccgcatcac gtcctgctcg accgggtctg gggctcggaa tgggccgggc
     5281 gaccggatta cctcaaggtg tttgtccgcc gcctgcgcca aaagctggat gacgacgccg
     5341 gccggccccg ttacatcgaa accgtctggg gcaccggtta tcgcttcctg ccatattcgg
     5401 catagcgccc gcgcccgggg ctacgtcacc ggtgcaaata gccagcctgg acgcttgttc
     5461 accggcggat caggcgttgt tggcgggcga ggcgcgcctg cagcaacacc acgatcagca
     5521 ggaaagcccc gcggatgatc tcttcccagt actgggtcag ctcaaaggtc cccttggccc
     5581 gctcgaagtt cagctcgttg aagatgaggc cgagcaggag gacgccgacg agcgaggtcc
     5641 agaccgagcc ctgcccgccg gtcagcagcg ttccgccgac cacgactgcc gcgatcgccg
     5701 agagctccca gccgatcgcg aagttggggg agccggtgta ggccagcgcc gccaggatcg
     5761 cgcccgccaa ccccgcgcac gctccgctga tcgcgtagat cgccactgtg gtgcgggcga
     5821 ccggcagacc ggccaggcgc gccgcctcct cgctgccgcc gaccgcaagc acatgccggc
     5881 cgaagcgggt gaagttgagc acgatcgacc cgatgatgta cgcgattacg gtgatcacga
     5941 ccggcagcgg cacgcgcccg atgttctttt ggccgatgtc aatgtaaccg ctggcaaagt
     6001 cgaccgagac gttggcattg tgcgccagat tgagcgcaaa gccgcgacag gccaagagca
     6061 tggccagcgt gacgatgaac ggctgaacct tgaaccgggc gatgatgccg ccgttgatga
     6121 tcccgatccc caaacccatt gcgaccgccc cgagcgtgcc gacccagatg ccgtgctcgc
     6181 tcaaccgggc cgcccaaacg gcagtcagcg ccgccaccgc accgaccgag agatcgatcc
     6241 cgccggccat gatcacgaag gtcatgccga gcgcgatcag gccgtagtag gagttgttgc
     6301 ggagcatctc ccagatgttg aaggagctgc ggaagttctc gccataccgg atcaggccga
     6361 aggcaaggat gatcagcagc gcgatcagcg cgccctgctg ctgtatcagc gatacggctc
     6421 gtgcaccgcg aggattcggg gcgacaggcg cctgggcaac catccagcct tcctcctcag
     6481 tcggaccggc gctgcaacag caacgccgcc aggatcaccg ctgcggtcac gatccgggtc
     6541 gtgccgtccg caacgccgtg tgagaggagc gtgtagcgaa tctggtggac gagcagcgcg
     6601 ccgaccaggg tgccaacgac cgtg
//
LOCUS       DASSGX010000534         2929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_639711, whole genome shotgun sequence.
ACCESSION   DASSGX010000534 DASSGX010000000
VERSION     DASSGX010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639711"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2154)
                     /locus_tag="VFI12_09655"
     CDS             complement(<1..2154)
                     /locus_tag="VFI12_09655"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HET7056716.1"
                     /translation="MFRQFRRVASKPTGTPRRPRTPIIPKITISKANKRRLIAAVVII
                     VLIAAVFLSAQFWMRWWWFGSMGYRSILLRRAALQIAAFLVVALLGFGIVGGSAALAF
                     RRTKNEARRSIFVRFANRVLFWLALAVSIVTGIGLAWWTANRWETLALWWNGRSMGIE
                     DPIYHRDASFYLFALPALDMLWNVAAIAALVGLALTTLVYLIRLGLNLRNVGSLPATA
                     FRHLLALIGVLLILGAVWFYLNNFHLVYSTRGAAYGVSYTDDHAIRIANWVAIIALLV
                     AGIACIASRRVPRRREAFLAALVVLFLSIGVQGLLPLVIQQAVVDPDELNKERPYITN
                     NIAMTRYAYGLGDVQAGELAGTGTITMADIDANPELLSNVRLWDYRVARTTYQELTSY
                     APYYQYLDVDIDRYVVNGVEQPVLVSARELNQAGLPSNAQTWTNRRLVYTHGYGAVVS
                     PVDEATSSGLPVFTVSQIPPNGTGPYTITYPEIYFGEADLGWVVVGTKVPEFSALPEV
                     DQTTSLTADDIVGGIRLDDSLTKLISSIALKDRNIFISGNIQDSSTLLLNRNIKDRIK
                     LIAPFLTLDPDPYLVIADGRLFWVVDAFTTSSKFPMSTPQDGINYIRASVKITIDART
                     GETIFYRTAEADPVADAYGRMFPSLFEPISTAPASIAAHFRYPEPIYDVQSRIYGAIH
                     VDDPDQYYNGEDRWTIPGSLASEDLLEDRSERDEMP"
     gene            2216..>2929
                     /locus_tag="VFI12_09660"
     CDS             2216..>2929
                     /locus_tag="VFI12_09660"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET7056717.1"
                     /translation="MMNPTDSTTEPAPSIRIRSLASGSSGNAYLLEAGDDLILIDCGI
                     GVRQIQAALASWGKSIRDISAIVISHEHSDHVRSLPSFLRHQTPILTTRGTANALGIA
                     HDRFAPIRYAEPVTVGSVSITPIETSHDAAEPCGLLIETSARTIGLITDLGTVTDGIG
                     DHACRCDLLILESNHDREMLRNGPYPAYLKRRVAGNHGHLSNDQAGGFLSEVATDCSG
                     PTEIWLAHLSATNNTPPVAK"
BASE COUNT          558 a          942 c          933 g          496 t
ORIGIN      
        1 cggcatctcg tcccgttcag accggtcctc gagcaagtcc tcggaggcca ggctgccggg
       61 aattgtccag cgatcctcgc cgttgtagta ctggtccgga tcatcgacgt ggattgcgcc
      121 gtagatgcgc gactggacgt cgtagatcgg ttccgggtag cggaaatggg ccgcgatcga
      181 cgccggcgcc gtggagatcg gctcgaacag ggatgggaac attcgcccat acgcgtcggc
      241 gaccggatcg gcctcggccg tgcggtagaa gatcgtctcg ccggttcgcg cgtcgatcgt
      301 gatcttcacg cttgcccgga tgtaattgat gccgtcctgc ggcgtcgaca tcgggaactt
      361 cgacgaggtc gtgaaggcat cgacgaccca gaacagccgt ccgtcagcga taacgaggta
      421 cggatccgga tcgagcgtca ggaacggcgc gatcagcttg atccggtcct tgatgttccg
      481 gttcaacaac agggtcgaac tgtcctgaat gttgccggag atgaaaatgt tgcggtcctt
      541 gagcgcgatc gagctgatca gtttggtcag cgagtcatcc agccggatcc cgcccacgat
      601 gtcgtcggcc gtcagtgacg tggtctggtc gacctccggc aaggccgaga actcgggcac
      661 cttcgtgccg acaaccaccc atcccagatc ggcctcgccg aaataaatct ccggatacgt
      721 aatcgtgtat gggccggtcc cgttcggcgg aatctgactg accgtgaaaa ccggcagacc
      781 gctcgaggtt gcctcatcga ccgggctgac gaccgcgccg tagccgtgag tgtagacaag
      841 gcgccggttc gtccacgtct gcgcgttgga cggcaacccg gcctgattca gctcgcgggc
      901 cgaaaccagg actggttgct ccacgccgtt cacgacatac cggtcaatgt cgacgtcgag
      961 gtactggtaa tagggcgcgt acgaggtcag ctcctggtag gtcgtgcggg cgacgcgata
     1021 atcccagagg cggacgttgg agagcagttc cgggttcgca tcgatgtccg ccatggtgat
     1081 cgtgccggtg ccggccagtt cgcccgcctg gacgtccccc agcccgtagg cataccgggt
     1141 catcgcgatg ttgttggtga tgtatgggcg ctccttgttc agctcgtccg gatcgacgac
     1201 cgcttgctgg atcacgagcg ggagtagccc ctgtacgccg atcgagagga acaggaccac
     1261 gagcgcggcc aggaacgcct cgcgccgccg gggcacgcgc cggctggcaa tgcaggcgat
     1321 gcccgccacc agcagcgcga tgatcgccac ccagttcgcg atccggatcg cgtggtcgtc
     1381 ggtgtagctg acgccgtagg ccgcgccgcg cgtcgaatag accaggtgga agttattcag
     1441 gtagaaccag actgctccga ggatcagcag cacgccgatc agggccagga ggtgccggaa
     1501 cgcggttgcc gggagagacc cgacgttgcg gaggttcaga ccgagccgga tcaggtagac
     1561 cagcgtagtc agcgcgaggc cgaccagggc cgcgatcgcc gcgacattcc agagcatgtc
     1621 gagcgccggc aaggcgaaga gataaaagct ggcgtcgcgg tggtaaatcg ggtcctcgat
     1681 ccccatggac cgaccgttcc accagagcgc gagcgtctcc cagcggttgg cggtccacca
     1741 ggccagcccg atcccggtga cgatcgaaac ggccagcgcc agccagaaga gcacgcgatt
     1801 ggcaaaccgg acgaagatcg agcgcctcgc ctcattcttg gtccgccgaa aggccagggc
     1861 ggcggatcca ccgacgatcc cgaagcccag cagggcgacg acgagaaatg ccgcaatctg
     1921 gagcgcggcc cggcgcaaca gaatgctgcg gtaccccatg ctcccgaacc accaccagcg
     1981 catccagaac tgggccgaca ggaagacggc cgcgatcaag acgatgatga ccacggccgc
     2041 gatcaaccgg cgcttgttcg ccttcgagat ggtgatcttc gggatgatcg gcgtgcgcgg
     2101 tcggcgaggt gttccggtcg gtttggaggc gacccggcga aactgtcgaa acatggtgat
     2161 tcggcgcact cctggtcaac atacgcgtcg cattgccgct catcgtacta ttcccatgat
     2221 gaatccgacc gactctacca ccgagcccgc accatcgatt cgcatccgga gcctcgccag
     2281 cggcagctca ggcaacgcct acctgctcga ggccggcgac gatctgatcc tgatcgactg
     2341 cggcatcggt gtgcgccaaa tccaggcggc gctggcgtca tggggcaagt cgatccggga
     2401 catctcggcg atcgtcatct cgcacgagca ctcagaccat gtgcgctcgc tcccgtcgtt
     2461 cctgcgccac cagacgccga tcctgactac ccgcggcacg gccaatgcgc tcggcattgc
     2521 ccatgatcga ttcgcgccga tccgttacgc cgaaccggtc acggttgggt ccgtgtcaat
     2581 tacgccaatc gagacgtcgc acgatgccgc cgagccttgc ggcctgctca ttgaaacatc
     2641 tgcccgcacg atcggactga tcaccgatct tggcaccgtc accgacggga tcggcgatca
     2701 cgcctgccgc tgcgacctgc tcatcctcga atcgaatcac gaccgcgaga tgctccggaa
     2761 tgggccgtac cctgcctatc tgaagcggcg cgtggcgggg aaccacggcc acctctcgaa
     2821 cgaccaggcg ggcggattcc tgtccgaggt cgcgaccgac tgctccggtc cgaccgaaat
     2881 ctggctcgcg cacctctcgg cgacgaacaa cacgccgcct gtcgcgaag
//
LOCUS       DASSGX010000535         2124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_968630, whole genome shotgun sequence.
ACCESSION   DASSGX010000535 DASSGX010000000
VERSION     DASSGX010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_968630"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1095)
                     /locus_tag="VFI12_09665"
     CDS             complement(<1..1095)
                     /locus_tag="VFI12_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056718.1"
                     /translation="MTYSDDALDRYLDALAMGTPVTSDQLDPELAETIHRLSELGRNP
                     EPHPGFVDQLEERLMNAVSDVPVVPLSPIRISRPGAPWVVPSRAARSTRGTRRWSPIL
                     IAAALILAVLGVAWRTFDSAGPRRNPQVGAPAIQAPATPSPGASRDQTLLEVTLPVDV
                     VPVGEDVNVLLARDTIPANSVTTHAPMDCCAGTKTYHILAGMVSFVSDGPVQRIRHGG
                     GGRLEEIAPGKSVELVPGDSLVVRNEHHQTWTTGTEPVDIITTAVASGFWMTSFDPIE
                     WVGNGSASNSQMISLPAGPYLLRIQRKEISEDATFPPTAGTIIQFGLTSDGTGVGQSS
                     DQTLSVKGVRGHMSVFILSLEVAPESVGTPV"
     gene            complement(1092..1697)
                     /locus_tag="VFI12_09670"
     CDS             complement(1092..1697)
                     /locus_tag="VFI12_09670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7056719.1"
                     /translation="MAGATSPHLQIVDPQSVPEPVGDDELVARAQGDPEAFAVLYRRY
                     VTPVYRYCRYRLSSPTAAEDATSEIFMKAFTGIRSYRNQCSFRSWLFAIAHNTVSDSY
                     RAYRPEEPIEAADGMHDPDPSPEDRALFAEGERSVQALLKRVTPQQARILELRLAGLT
                     GPEIAQVLGCSLAAVKIGQVRGYGRLREVLAGSPNAEGARS"
     gene            complement(1836..>2124)
                     /locus_tag="VFI12_09675"
     CDS             complement(1836..>2124)
                     /locus_tag="VFI12_09675"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET7056720.1"
                     /translation="RARNYAPDVVVLCSYGALRGLDAMRRSAADLKEQLPNARFDYKL
                     HEVEGEYGYLLWSAESDTCRVPEGADSYVMRNGRIVMQSIYYRLEPGNPGS"
BASE COUNT          373 a          666 c          711 g          374 t
ORIGIN      
        1 gacgggtgta ccgacgctct cgggagccac ctccaggctc agtatgaaga cggacatgtg
       61 gccccgtacg cccttgacgg agagtgtctg atcactcgac tggccgacgc ccgtgccatc
      121 gctcgtcaac ccgaactgga tgatagttcc cgctgttggc gggaatgtcg cgtcttccga
      181 aatctctttc cgctgaatgc gcagcagata tggtccagcc gggagcgaaa tcatctggct
      241 gttagaggcg cttccgttgc caacccattc gatcgggtcg aaggacgtca tccagaaccc
      301 gctggcgacc gcagtcgtga taatgtcgac cggctctgtg cctgttgtcc acgtctggtg
      361 gtgctcattg cggaccacga gtgagtcccc gggcactaac tcgacgctct tgccaggcgc
      421 gatctcctcg agcctgcccc cgccaccgtg ccggatccgc tggaccggac catcgctcac
      481 gaatgagacc atgccggcga ggatgtgata tgtcttggtg ccggcgcagc aatccatggg
      541 tgcatgggtt gtcaccgaat tggccgggat cgtatcgcgc gccagcagga cgttaacgtc
      601 ttcgccgacc ggcacgacat ccacaggcag cgtcacctcc aacaaggtct ggtcccggga
      661 tgcaccgggt gagggcgtgg ccggcgcctg aatggccggc gcaccgacct gcggattgcg
      721 gcgagggcca gcagaatcga acgttcgcca cgccactccc agcaccgcga ggatgagcgc
      781 ggctgctatc aggatcggcg accagcggcg agtgccccgc gtcgacctgg cggcgcgact
      841 cgggacaacc cacggtgcac cgggtcgact gatgcgaatc ggggacaagg gaaccacagg
      901 aacatctgac acggcgttca tgagcctctc ctccaattga tcgacgaacc cgggatgcgg
      961 ttctgggttc cggccgagtt cgctgagacg gtggattgtc tcggcgagct cggggtcgag
     1021 ctggtcgctg gtcaccggcg tgcccatagc gagggcatca aggtagcggt cgagtgcgtc
     1081 gtcgctgtat gtcatgaccg cgctccctcc gcattcggag atccggccag gacttcccgg
     1141 agccggccgt acccgcgcac ctggccgatc ttgacggcgg cgaggctgca cccgagtacc
     1201 tgcgcaatct cgggcccggt caacccggcg agccgcagct cgagaatacg cgcctgctgc
     1261 ggcgtgaccc gcttcaggag cgcctggacc gagcgctctc cctcggcaaa gagcgcccgg
     1321 tcctccggag agggatcggg gtcgtgcatg ccgtcagcgg cctcgatcgg ttcctctggc
     1381 cggtaggcgc ggtagctatc ggagacggtg ttgtgggcga tcgcgaagag ccagctccgg
     1441 aacgagcact ggttgcgata gctgcggatt ccggtaaaag ctttcatgaa gatctcactg
     1501 gttgcgtctt cggccgccgt cggcgaagaa aggcggtacc ggcagtagcg gtagacaggg
     1561 gtgacgtagc ggcggtagag gacggcgaac gcctccgggt cgccctgggc gcgggcgacg
     1621 agctcatcgt cgccgaccgg ctcggggacc gactgcgggt caacgatctg gagatgtggc
     1681 gaggtcgctc ccgccatcct ggtgtctccg ttcgcgtttc agcatccatc tgacagggta
     1741 gacgggaaac ctggcaaatt ggatacaccc ggcggtattg ccaccgccca ggcggggtgt
     1801 gacgagcccc tggaactgat tggtatggac gctggttacg acccggggtt gcccggttcg
     1861 aggcggtaat agatggattg catgacaatc cggccgttcc gcatcacgta ggaatcggcg
     1921 ccttccggca cgcggcatgt gtccgactcg gccgaccaga gcaggtagcc gtactcgcct
     1981 tcaacctcgt gcagcttgta atcgaaccgg gcgttcggga gctgctcctt gagatcggcc
     2041 gccgagcggc gcatcgcgtc caggccacgc agcgcgccgt acgagcagag cacgacgaca
     2101 tccggcgcgt agttgcgagc acga
//
LOCUS       DASSGX010000536         3527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1403423, whole genome shotgun sequence.
ACCESSION   DASSGX010000536 DASSGX010000000
VERSION     DASSGX010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403423"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VFI12_09680"
     CDS             complement(<1..396)
                     /locus_tag="VFI12_09680"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET7056721.1"
                     /translation="MSPAAALTQTSPMIVAIVEDQAAIAELLYDVLEDAGYAPVITSS
                     PVNAAAEIAEFEADIVLLDIMMPRKDGWEVLDELRAEPATRDVPVILMSAVCRDGYSE
                     YYASVSLRAEAYFVKPFAMGAMLSRIRELL"
     gene            complement(393..899)
                     /locus_tag="VFI12_09685"
     CDS             complement(393..899)
                     /locus_tag="VFI12_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056722.1"
                     /translation="MSPSEGIVPAPDIPATVTTIRLASLVRDDSTRILANWSMRIATL
                     PVFRALPELALDELQEDMPALLAAVLEAVSVSPYELDPAPMEEAEAKADAHGAKRAAS
                     VPVDVLLIEIQTLQREVRNAIWRNADEAPVAIVHELDERLNEVFELAERSAVSAWVRQ
                     RTQEGVAA"
     gene            1222..2823
                     /locus_tag="VFI12_09690"
     CDS             1222..2823
                     /locus_tag="VFI12_09690"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HET7056723.1"
                     /translation="MTADLVVANARIRTLDTDRPSATAIAITNGIITAVGDDPDIRDQ
                     IGPRTEVIDGNGLAIVPGLTDSHFHPFWGAEATQGVDLTKATTLDDLRALLRAERDRV
                     GPDAWVSGWGLTFEMFQETGIRGDTFADAVDGGLAFLAFFDGHTAVVSPAAIAFADVT
                     GRETFPDYSVVVTDEHGVPTGELREGGAMELVRRAIPKPTAADKYRWYVEAMRKWNAV
                     GLTGVHAMDGSPETFELLRQMEARGDLSVRMIVPLWQHPEFSFDQMREQLPCRDERGE
                     LWRGGVAKFFIDGVIESGTGWLIEPDTKGMGTAPFWPDPARYQEAVRIFAEAGFQCAT
                     HACGDMAVRCALDAYEQSGAAEGIRHRIEHIEILSDTDLPRFAKLGVTASMQPLHMNA
                     FEPDGSDEWSLRAGPERRKQAFRTRDLLESGATLALGSDWMVASYDPRVGMAWARSRR
                     PAGMQGRGPINGEQALTGLEALAGYTTGAAGAVSEEHLSGTIKVGYRGDLTAFAADPA
                     DTDPDELINLPVLLTVVNGRVVHRI"
     gene            2920..>3527
                     /locus_tag="VFI12_09695"
     CDS             2920..>3527
                     /locus_tag="VFI12_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056724.1"
                     /translation="MTQNMGTLDIEQPGALASYLREHDLITPGEEPRVTVLAGGVSNR
                     TVLVERQSGEAWVVKQALAKLRVAVDWFSSPERIHREAAGLRWLVEFAPPGSITPLVF
                     EDRSDHLLGMSAIPQPHENWKAMLLRGDLIADHVRQFATLIGQIHRQSHRRLAELAPV
                     FGDRTFFETLRVEPYYRYTASQVPESAGFYAALIDDTYARQDT"
BASE COUNT          613 a         1184 c         1149 g          581 t
ORIGIN      
        1 gagcagctcg cggatgcgcg agagcatcgc gcccatggcg aacggcttga cgaagtacgc
       61 ctccgcccgc agcgacaccg acgcgtagta ctccgagtag ccgtcgcgac agacggcgct
      121 catcaggatc accggcacgt cgcgcgtggc cggctcggcg cggagctcgt ccagtacctc
      181 ccacccatcc ttgcgcggca tcatgatgtc gagcaggacg atatcggcct caaactcggc
      241 aatctccgcg gcagcgttca ccggggagct ggtgatgacc ggcgcgtaac cggcatcctc
      301 cagcacgtcg taaagcagct cggcgatcgc ggcctgatcc tcgacgatcg ccacgatcat
      361 cggcgatgtt tgtgtgagtg cagcagcggg actcatgccg caaccccttc ctgcgttcgc
      421 tgccggaccc atgcgctcac cgcgctacgc tcggccagct cgaatacctc attcaaccgc
      481 tcatccagct catgaacgat cgcgaccggc gcctcatccg cgttccgcca gatggcattc
      541 cggacttcgc gctggagcgt ctgaatttcg ataagcagca catcgaccgg cacgctggcc
      601 gcccgcttgg cgccgtgcgc gtcggccttg gcctccgctt cctccatcgg agccgggtcg
      661 agctcatagg gcgagacgct gaccgcctcg aggaccgcgg cgagcagcgc cggcatatct
      721 tcctgcagtt cgtccagcgc tagctcgggc aatgcccgga agacgggcaa cgtggcgatg
      781 cgcatcgacc aattggccag gatgcgcgtg ctgtcgtcgc ggacaaggct cgcaagacga
      841 atcgtggtga ccgtcgccgg gatatcaggt gccgggacga ttccttcaga cggactcatc
      901 acagcctccg gttctcgatc gatcggtgtc aggacactac cagccatgca ttcctcaccg
      961 gtcttggcgt acgggacatg cgccaatcgc ataccgttgc ttgctgcaca gcctatgctc
     1021 acccacgccg tgacgtatct acccgacagc gtgagttcgc gtagcccgat tggcacagga
     1081 ccgtttggct ttgatcgtac gttcacgtcc caattcctgt gcaaatcatg aacgtgatgc
     1141 acatgaaaat gccggatttt cgtgagtttg cgaagattac gcggtgatcg atcagggcaa
     1201 ttcgtaccag ggggcaaacc gttgaccgca gacctcgttg tagccaacgc acgcattcgc
     1261 acacttgaca ccgatcgccc aagcgccacg gcgatcgcca tcacgaacgg catcatcacc
     1321 gccgtcgggg acgaccccga catccgcgat cagatcggcc cgaggaccga agtgatcgac
     1381 gggaatggcc tggcgatcgt tccgggcctg accgacagcc acttccatcc cttctggggc
     1441 gctgaagcca cgcagggcgt cgacctgacg aaggcgacga ccctcgatga tctgcgcgcg
     1501 ctgctccggg ccgagcggga tcgggtcggg ccggacgcat gggtgagcgg ttggggcctt
     1561 accttcgaga tgttccagga gaccggcatc cggggcgaca ccttcgccga cgcggtcgac
     1621 ggcgggctcg ccttccttgc cttcttcgac ggccacacgg cggtcgtctc gccggccgcg
     1681 atcgcgtttg ccgatgtcac cggccgcgag accttccccg attactccgt cgtcgtcacg
     1741 gacgagcacg gcgtgccgac cggagaattg cgcgagggcg gcgcgatgga gctggtccgc
     1801 cgcgcgatcc cgaagccgac cgccgcggac aagtaccgct ggtacgtcga ggcgatgcgg
     1861 aagtggaacg ccgtcggcct gaccggcgtt cacgccatgg acggctcgcc ggaaaccttc
     1921 gagctgcttc gccagatgga ggcgcgcggc gacctgagcg tgcggatgat cgtcccgctc
     1981 tggcaacatc ccgaattcag cttcgatcag atgcgggagc agcttccctg ccgggatgag
     2041 cgcggcgagc tctggcgcgg cggcgtggcc aagttcttca tcgacggcgt gatcgagtcc
     2101 ggcaccggct ggctgatcga gccggacacg aaggggatgg gcacggcgcc attctggccc
     2161 gatcccgcca ggtaccagga ggcggtccgg atcttcgccg aggccgggtt ccagtgcgcc
     2221 acccacgcct gtggggacat ggcggttcgc tgcgcgctcg atgcctacga gcaatccggc
     2281 gcggccgagg gcatccgcca ccggatcgag cacatcgaga tcctgtcgga tacggatctg
     2341 ccccggttcg caaagcttgg cgtgacggcc tcgatgcaac cgctgcacat gaacgccttc
     2401 gagccggacg gaagcgacga gtggagcctc cgcgctggtc ccgagcgacg gaaacaggcc
     2461 ttccgcacgc gtgacctgct cgagagcggc gcgacactcg cgctcggctc ggactggatg
     2521 gtcgcgtcct atgacccgcg cgtcggcatg gcctgggcgc gctcccggcg gccggccggg
     2581 atgcagggcc ggggcccgat caacggcgag caggcgctga ccggcctcga ggcgctcgcc
     2641 gggtacacga ccggggcggc cggggctgtc tccgaggaac atctgagcgg gacgatcaag
     2701 gtcggctacc ggggcgacct gaccgccttc gccgccgatc cggctgacac agaccccgac
     2761 gagctgatca atctcccggt cctgctcacg gtcgtcaacg gcagggtggt gcatcgtatc
     2821 tgaggcgcgg agtcgggagg cgcgaaggcg cggagttgta gcgtcgcatg gcaatgcgac
     2881 cccggccctc cacccgaggc gcgggaacta actgatccga tgacgcagaa catgggcacg
     2941 cttgatatcg aacaacccgg cgcgctggcc agctacctgc gggagcatga cctgattacg
     3001 cccggcgagg agccgcgggt gacggtgctg gccggtggcg tctcgaaccg gacggtgctg
     3061 gtcgagcgcc aatcgggcga ggcgtgggtc gtcaagcagg cgctggccaa actgcgggtc
     3121 gcggtcgact ggttcagcag cccggagcgt atccaccgcg aagccgccgg cctgcgctgg
     3181 ctggtcgaat ttgctccgcc gggtagcatc acgccactcg tcttcgagga tcgatcggat
     3241 cacctgctcg ggatgtcggc cattccccaa ccgcacgaga actggaaggc gatgctcctg
     3301 aggggcgatc tgatcgccga tcatgtccgc caattcgcga cgctgatcgg gcaaatacac
     3361 cgccagagcc atcgccgcct cgccgaactt gcacctgtct tcggcgaccg cacgttcttc
     3421 gagacgctgc gagtcgagcc gtattaccgg tacaccgcca gccaggtccc ggaatctgcg
     3481 ggtttctacg ccgccctgat cgatgacacc tacgcgcggc aggacac
//
LOCUS       DASSGX010000537         2365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_640130, whole genome shotgun sequence.
ACCESSION   DASSGX010000537 DASSGX010000000
VERSION     DASSGX010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640130"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2365)
                     /locus_tag="VFI12_09700"
     CDS             complement(<1..>2365)
                     /locus_tag="VFI12_09700"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET7056725.1"
                     /translation="AGGTQYGTEIANALRDAAAVVLIASDASLASKNVRQEIMLAWRY
                     DRPIIPLILHPLVFPDDVAYWLEGAQWIEVFDRAPEAWVPKLWQALARHGFATVPDTP
                     AAPAATPAFAQAGNLPARLPPILGRDREIEEVIALLRNGRTITLTGPGGTGKTRLALE
                     VGRRVGPGYEGGAWFLDLSAVRSAELVAPTTAAMLELDVPPGASPIEILAAHLGRRAT
                     LVVLDNLEQIPEVVGDLAALQAAAPGLSLLMTSRASMQLPGGWVYAVPQLALPDLSNL
                     PAPATLMENPAVRLFVDRATLARSDFQLIDGNARAVAEICHRLDGLPLAIEIAAARTR
                     MLPPAAILTRLGSRLNLLTRGSAANARQQTLRDTIAWSYDLLDSEAQRAFRTCAVFAG
                     GTPVEAAEAVVSEIAGETQPALDLLEHLIDQSLLVTETRDDGATALRMLETLREYAEE
                     QLEQHGESDSVHAAHLQWYLDWIESLTPELRARRDGGAIRAVRAELGNLRAALAWVDG
                     HDTGAARLRLTSALFHYWRTAGPFAEAYEELAGALAAGGDTPRVELGQVASALAWLAA
                     ARGDFGEARTLYRRALEIYEAVGDVPHQAETHTDLALVAEFTADYAGAREHLDQRRLL
                     LPPDDVRALAQIEHDLGRLTFIEGEFPEAIRSISGAISTYRMLADTQLIAIALIDLAS
                     AELLNGDAADSLAHIRESIALLRTLQDDYARVVAEATRGRAEQLAGDYTGAQATLEQA
                     LVDAETLGDVSLRALALYGLGVNATFLEDWEGAEARLREAFAISHEIG"
BASE COUNT          416 a          905 c          680 g          364 t
ORIGIN      
        1 cgccaatctc atgagaaatc gcgaacgcct cgcgcagccg cgcttcggcg ccctcccagt
       61 cctccagaaa cgtcgcgttc accccgagcc catagagcgc cagcgcccgc agactgacat
      121 cgcccaacgt ctccgcatcg acaagcgcct gttccaatgt cgcctgggcg ccggtatagt
      181 cgccagcaag ttgctccgcc cgcccgcggg ttgcctcggc caccacccgc gcatagtcgt
      241 cctgcaaggt tcgcagcagc gcgatgctct cccgaatatg cgccagcgag tcggccgcat
      301 caccgttgag cagctcggcc gacgccagat ctatgagcgc gatggcgatc aattgcgtat
      361 cggccagcat tcgataggtc gaaattgccc ccgaaatcga gcggatagcc tccggaaact
      421 ccccttcgat gaacgtcaac cgccccagat cgtgctcgat ctgggccagc gcgcgcacat
      481 cgtcgggcgg caagagcaac cgccgctgat ccagatgctc tcgcgctccg gcataatcgg
      541 cggtgaattc cgccaccagc gccaggtcgg tatgggtctc cgcctgatgc ggcacatcgc
      601 caacggcctc gtagatctcc agcgcccggc ggtagagcgt gcgcgcctcc ccgaaatccc
      661 cgcgcgccgc ggctagccac gccagcgcgc tcgccacttg cccaagctcg acccgcggtg
      721 tatcgccacc cgccgccagc gcgcccgcca gttcctcata cgcttcggca aacggcccgg
      781 cggtccgcca gtaatggaac agcgcgctgg tcagtcgtag gcgggccgcc ccggtatcgt
      841 gtccatcgac ccatgccaat gccgcccgca aattccccag ctcggcccga accgcccgaa
      901 tggcgccccc gtcgcgtcgg gccctcagtt cgggcgtcag cgactcgatc caatcgagat
      961 accactgcag atgcgccgca tggacgctgt ccgactctcc atgctgctcg agctgctctt
     1021 ccgcgtattc acgcaacgtc tcgagcatgc gcagcgccgt cgcgccgtcg tcgcgtgttt
     1081 cggtcaccag cagactctgg tcgatcaagt gctcgagcag gtcgagcgcc ggttgcgtct
     1141 ctcccgcaat ctcgctcacg actgcttccg ccgcctccac cggcgtccct ccggcaaaaa
     1201 ccgcacacgt gcgaaacgcg cgttgcgctt cagaatcaag caagtcgtac gaccaggcga
     1261 tcgtatctcg cagcgtctgc tgccgcgcat tggccgcgct accccgcgtg agcagattca
     1321 gacggctacc caatcgcgtg aggatcgccg ccgggggcag catccgcgtg cgcgccgcgg
     1381 caatctcgat cgccagcggc aacccatcga gccggtggca aatctccgcc accgcccgcg
     1441 cattgccatc gatcaactga aaatcggacc gtgccagcgt ggcgcgatcg acgaagagtc
     1501 gcaccgccgg gttctccatc aacgttgccg gcgcgggcag attgctcaga tcgggcaggg
     1561 ccaactgcgg aacggcatac acccaccctc ccggaagctg catcgacgcc cggctcgtca
     1621 tcagcaacga cagccctggc gcggccgctt gcagcgccgc caaatccccg accacctccg
     1681 gaatctgctc caggttgtcg agcacgacca gcgtcgcccg cctgccgaga tgagccgcca
     1741 ggatctcgat cggcgacgcc ccgggtggga cgtcgagctc gagcatcgct gcggtagttg
     1801 gcgccaccag ctcggccgac cgtaccgcgg aaaggtcgag aaaccaggcg cccccctcat
     1861 accccggacc aactcgccgc cccacctcca gcgccagccg ggtctttccc gttccccccg
     1921 gtccggtcaa cgtgatcgtc cgcccgttgc gcagcagagc gatgacctcc tcgatctcgc
     1981 ggtcccggcc cagaatcggc ggcagcctcg ccggcagatt gccggcctgc gcgaatgccg
     2041 gcgtcgcggc cggggccgcg ggagtatccg gcacagtcgc aaacccgtgt cgcgcgagcg
     2101 cctgccacag cttcggcacc cacgcctccg gcgctcggtc gaacacttcg atccactgcg
     2161 cgccttcgag ccagtaggca acatcgtccg gaaaaaccag cggatgcaga atcagcggaa
     2221 tgatcggccg gtcgtaacgc caggccagca tgatctcctg ccgcacattc ttcgaagcca
     2281 gcgaggcatc cgaggcaatc agcaccaccg ccgccgcatc ccgcagcgca ttggcgatct
     2341 ccgtgccgta ctgcgtacct cccgc
//
LOCUS       DASSGX010000538         2217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_97026, whole genome shotgun sequence.
ACCESSION   DASSGX010000538 DASSGX010000000
VERSION     DASSGX010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97026"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1607
                     /locus_tag="VFI12_09705"
     CDS             <1..1607
                     /locus_tag="VFI12_09705"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET7056726.1"
                     /translation="GIISIVVASGLAALVARVTEMSFIVTQMISMIGLAVGIDYALFI
                     VERYREERRHGRPKLAAIEVAGGTASKAVLFSGMTVVFALIGLFLIPTTIYRSLGIGA
                     ALAVTVAVAAMLTLIPAILALLGDKVDWPRKHRYDDREQIASQERRDAETIHGGFWGT
                     ITRVVMARPVVALILSVGVLVTLAIPALDLRTGNNGAESLPPSDVRRGFDILASPNGF
                     DTAGRSQPIMIAIAGTTSEIQAGIDRLDAAFAADPATFAVIAEPRWSASYDFAVVDLY
                     LRTDANSQGTRDTITALRETVVPAAFSGLSADVYVTGEAAFNFDFGTVVDQWTPWVFA
                     FVLGLSFVLLTMAFRSIIVPAKAIIMNLLSVGAAYGLLVAVFQKGYLHTALGFQQTPT
                     IEAWLPIFLFCVLFGLSMDYHVFLISRIREHYDATRNNAESVAVGLQATAKIITGAAL
                     IMVAVFGGFAAGRLAPLQQLGFGLGAAILIDATVIRSILVPSAMTLLGDRNWYMPRWL
                     TWLPDLRVEGTPAVPVATFPIAAPAD"
     gene            complement(1746..>2217)
                     /gene="rpiA"
                     /locus_tag="VFI12_09710"
     CDS             complement(1746..>2217)
                     /gene="rpiA"
                     /locus_tag="VFI12_09710"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HET7056727.1"
                     /translation="LSGVDSIDIGFDGADEIDPDLNLVKGKGGALLYEKLVAEICRDY
                     MVVSADEKLVARLGTRLPLPVEVIPYGWIHTRRRLADLGLTPEIRGGGENPFHTDAGN
                     VILDCTPESGSDLLALAPRIKAITGVVEHGVFSGLARRAAIVDAGGRVEFLIKA"
BASE COUNT          361 a          697 c          720 g          439 t
ORIGIN      
        1 tcggcatcat ctcgatcgtc gttgcgagtg gtctggccgc acttgtcgcc cgcgtgaccg
       61 agatgtcgtt catcgtgacc cagatgattt ccatgatcgg tctggctgtc gggatcgact
      121 acgcgttgtt catcgtcgag cgctatcgcg aggagcggcg gcacggtcgg ccgaagctcg
      181 cggcgatcga ggtcgccggc ggcacggcca gcaaggcggt cctcttctcc ggaatgacgg
      241 tagtgttcgc cttgatcggt ctcttcctta tcccgaccac catttaccgc agccttggga
      301 tcggcgcggc cttggccgtg accgtagcgg tggcggcgat gctcacgttg atcccggcga
      361 tcctcgcatt gctgggcgac aaggtcgatt ggccacgcaa gcatcgctac gatgatcgcg
      421 agcaaatcgc gtcgcaggag cgacgcgacg ccgagacgat tcacggcggg ttctggggaa
      481 ccattacccg ggtcgtaatg gcgcggccgg tggtcgcgct catcctctcg gtaggcgtcc
      541 ttgtcacgct ggcgattccc gctctggatc tcaggacggg caataatggt gccgaatcgt
      601 tgccaccgag cgatgttcgg cggggcttcg atattctcgc ctccccgaac gggttcgata
      661 cggcgggccg gagtcagccg atcatgatcg cgatcgccgg caccacgtcg gagatccagg
      721 ccggcatcga cagactggac gcggcgttcg cggcggatcc ggcaaccttt gcggttatcg
      781 cagagccccg gtggagcgcg tcctatgact tcgcggtggt cgatctctac ttgaggacag
      841 acgcgaactc gcagggcact cgtgacacga tcactgcgtt gcgagagacc gtcgtcccgg
      901 ccgcctttag cgggctatcg gcagacgtct acgtaaccgg cgaagcggcc tttaatttcg
      961 atttcggtac ggtcgtcgat cagtggacac catgggtgtt cgcgttcgtc ctcgggctca
     1021 gcttcgtcct gctgaccatg gccttccgct cgatcatagt gccggccaag gccatcatca
     1081 tgaacctgtt gtcagtgggc gcagcctacg gactgctggt ggcagttttc cagaagggct
     1141 acctgcacac cgcgctcggc tttcagcaga caccgacgat cgaggcgtgg ttgccaatct
     1201 tcttgttctg cgtgctcttc gggctctcga tggattacca cgtcttcctg atcagccgca
     1261 tccgcgagca ctatgacgcc accaggaata atgccgaatc cgtcgcggtg ggcctccagg
     1321 cgacggcgaa gatcatcact ggtgcggcgc tgatcatggt cgcggtgttc ggcggcttcg
     1381 ccgccggccg gctggcgccg ttacagcaat tgggcttcgg actcggcgct gcgatcctga
     1441 tcgacgccac agtcatccgc tcgatcctgg tcccgagcgc gatgacgctg ctcggtgacc
     1501 ggaactggta catgccgcgt tggttgacct ggctgcccga cctacgggta gaagggaccc
     1561 ccgccgttcc ggtcgccacc ttcccgattg ccgctcccgc cgactgaaat gtgtcccgac
     1621 tgacgcgctc tgccgatcgt cacctccggt cgttccggca gtgacgatcg gtgcgtgcgg
     1681 cctcggaccg ctcgatgctc cggccgcgtg tcataaggac gggtgagcgc ggaaatggct
     1741 tgaggctagg ccttgatcag gaactcgacg cggccgccgg cgtcgacgat cgccgcccgg
     1801 cgggcgaggc cggagaagac gccgtgctcg acgacgccgg taattgcctt gatccgtggt
     1861 gcgagcgcga gcaggtcgga acccgactca ggggtgcaat cgaggatgac gttgccggcg
     1921 tcggtgtgaa agggattctc gccaccgccc cgaatttcgg gcgtcaggcc cagatcggcg
     1981 agacgtcggc gcgtgtggat ccagccgtag gggatgacct cgactggcaa gggtaaccgg
     2041 gtaccgagac gggcgaccag cttctcgtcg gccgagacaa ccatgtaatc gcggcagatc
     2101 tcggccacga gcttctcgta gagcagcgcc ccgcccttgc ccttgacgag gttcagatcc
     2161 gggtcgatct cgtccgcgcc atcgaatccg atgtcgatcg aatcgacacc ggacagg
//
LOCUS       DASSGX010000539         2001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_530763, whole genome shotgun sequence.
ACCESSION   DASSGX010000539 DASSGX010000000
VERSION     DASSGX010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530763"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..687
                     /locus_tag="VFI12_09715"
     CDS             226..687
                     /locus_tag="VFI12_09715"
                     /inference="COORDINATES: protein motif:HMM:NF019003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050485 - oxidoreductase activity, acting
                     on X-H and Y-H to form an X-Y bond, with a disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase
                     selenoprotein B family protein"
                     /protein_id="HET7056728.1"
                     /translation="MVRLSDVPPEMAEGWRKTPMPTFETSPWVPGPDLRLARVAIVTT
                     AGLHRRDEAKFTGGASDYRIIPDDVDFADLVQSHVSVNFDRTAFQQDANAVFPLQHLH
                     NLAQAGDIGSVARWHYSFMGATDPSRVVDSGKQVADLLKHDGVDAVILVPV"
     gene            691..1470
                     /locus_tag="VFI12_09720"
     CDS             691..1470
                     /locus_tag="VFI12_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056729.1"
                     /translation="MCTRTVGSLGHYIEEEGLATTGISLIREHTEQIKPPRALWVPFE
                     LGRPFGPPNNPEFQLGVLHRLLDLFAEPSGPVLRDYPIDAPARAAGDEPWSCELPLPP
                     LEDASTPEDRLRVSFLQEIGLMRPWYEEALREHRRTAIGLSGLSPDRIDEAATFVAAW
                     ATGETPPLPDGVAGPELGALRFIADDLKSFYLEAASVQPGAGTAMSRELNAWLYHGTW
                     FGQALYDVRDRLTREAEAMNDPARGRIALVPGAFRERPPRR"
     gene            1545..>2001
                     /locus_tag="VFI12_09725"
     CDS             1545..>2001
                     /locus_tag="VFI12_09725"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056730.1"
                     /translation="MVMSDDLGPIAKNVEAALQALSREDAVARLWRGDYTLWGNDPTE
                     ITEPNRLGWLEVADSMQGDLDDLRAFAKDVAAAGYMTAVLMGMGGSSLAPEVLQATFG
                     TTKGMLKVHVLDTTHPDSILDLTHAIDIDKTLFIVSSKSGTTVETLSHFD"
BASE COUNT          363 a          690 c          589 g          359 t
ORIGIN      
        1 aaccggcctt ggaaaccaga gcgcatgtgt gcatgtcgcc gatcaggccg tagtcgccga
       61 tcggaggata tgcgccgctc tcattcatat aggtacatca tgactaactc cacccaaccg
      121 cgaaaacccc gtattcgtat catgcatcta gctaatagcg gcccacctat ttatcgacgc
      181 tctcaatcgc ttcgtgcccg ccgtccttgg gggtatattg ccagcatggt ccgactgtcc
      241 gacgtaccgc cggaaatggc agagggctgg cgcaagacgc cgatgccgac cttcgagaca
      301 tcgccctggg tgcccggtcc ggacctcagg ttggcgcgcg tcgcgatcgt tacgaccgcc
      361 gggttgcacc gccgcgatga ggccaagttc actggcggcg ccagcgacta tcgcatcatc
      421 ccggacgacg tcgacttcgc cgacctcgta caaagccacg tcagtgtcaa cttcgaccgc
      481 acagcctttc agcaggacgc caatgcagtc tttccactgc agcatctgca taatctggca
      541 caggccggcg acatcggctc cgtcgcgcgc tggcactact cgttcatggg cgcgaccgac
      601 ccgtcgcgcg tcgtcgactc cggcaagcag gtcgcggacc tgctcaagca cgatggcgtc
      661 gacgccgtca tcctcgtccc cgtctgaccg atgtgcacgc ggaccgtcgg ttcgctcggc
      721 cactacatcg aagaagaggg tctggcgact accggcatca gtctgattcg cgagcatacc
      781 gagcagatca agccgccgcg cgccctctgg gtgccgttcg agctcggtcg ccccttcggc
      841 ccgcccaaca acccggaatt ccagctcggc gtgcttcatc gcttgctcga cttgttcgcc
      901 gagccctccg gtcccgttct gcgtgactac ccgatcgatg cgccagcccg cgccgcgggt
      961 gacgagccat ggagctgcga gctaccgctg ccgcccctcg aagacgcttc gacgccggaa
     1021 gatcgcctgc gtgtgtcatt cctgcaggag atcggcctca tgcgaccctg gtacgaagaa
     1081 gcccttcgcg agcacaggcg caccgccatc ggcctttcgg gtctgtcccc ggaccgtatc
     1141 gatgaagctg ccacgttcgt agcggcctgg gccaccgggg aaacgccgcc gctgccagat
     1201 ggagtcgctg gtccggagct cggagccttg cgcttcatag cggacgacct caagtcgttc
     1261 tacctcgagg ccgcatcggt acagcccggc gcagggacgg caatgtcgcg agagctcaac
     1321 gcctggctct accacggcac ctggttcggg caggcgttgt acgacgtgcg cgaccgcctc
     1381 acccgcgagg ccgaggcaat gaacgacccg gcgcgcggcc gcatcgccct cgtgcccggc
     1441 gctttccgcg aacgtccgcc gcgtcgctag accctctgtt ctcattccgc accggcgacc
     1501 gttacgcagc cttaacgccg tgggctgggt aacgggtcta aactatggtc atgtcagacg
     1561 atcttggacc aatcgctaaa aatgtcgaag ccgcgttgca agcactgagc cgcgaggatg
     1621 cggtggcgcg cttgtggcgc ggcgattaca ccctctgggg caacgacccc accgagatca
     1681 cggaaccgaa tcgactcggc tggctggaag tcgccgattc gatgcagggc gatctcgatg
     1741 acctgcgcgc cttcgcgaag gacgtcgcgg cggccggcta catgacagcc gtgctcatgg
     1801 gcatgggggg ctccagcctg gcgcccgagg tgctacaggc cacgttcggc accaccaagg
     1861 gaatgctcaa ggtgcacgtg ctcgacacga ctcatcccga cagcatcctc gacctcacac
     1921 acgctatcga tatcgacaag acgctgttta tcgtctcgag caagtcaggt accaccgtcg
     1981 agacgctctc gcacttcgac t
//
LOCUS       DASSGX010000540         4276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_203988, whole genome shotgun sequence.
ACCESSION   DASSGX010000540 DASSGX010000000
VERSION     DASSGX010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203988"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(288..815)
                     /locus_tag="VFI12_09730"
     CDS             complement(288..815)
                     /locus_tag="VFI12_09730"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HET7056731.1"
                     /translation="MFATATALAIMLAFAALVTFGGSPAVAQDSTSVTIQNFAFSPNS
                     VTITAGSSVTWTNMDSVTHTATGDDGSFDTGDIAAGSSATITFNDPGTFTYHCAIHPN
                     MTGTIVVQAAGGGGGGNTGGNSGGGTSGGGTTQQLPNTGSGGLPGSGLDLAAYIVLAG
                     LTIGIAGFAMRRRMA"
     gene            complement(1289..1642)
                     /locus_tag="VFI12_09735"
     CDS             complement(1289..1642)
                     /locus_tag="VFI12_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056732.1"
                     /translation="MQTVLYVSPMELGNEVQVRDIHERFPAEALDSGIGIERLVAYIG
                     SGYYALEITVADGDFQERFRAFLTNPDVRRFFQELSAHVQDLPIASEGTAEMPLATAM
                     LEWSRGGGVAIDRTH"
     gene            1783..2826
                     /locus_tag="VFI12_09740"
     CDS             1783..2826
                     /locus_tag="VFI12_09740"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HET7056733.1"
                     /translation="MAADSALLWLGMQICGELSVVEEQSNRGASKRRDHPRGYVCPRT
                     TVTPRLDGRMDEAIWGHAPWTEEFVDIEGELRPNPRYRTRVRMLWDDDCLYIGAEMEE
                     PHVWGTLTDHDCVIYNDNDFEVFLDPNGACQHYVELELNALNTTWDLMLTRPYRVGGR
                     AISGWEFHGLRTAVSIDGTLNDPSDIDRGWKTEIAIPWSSLRDVTDRDLPPKSGDQWR
                     INFSRVEWQHDVIDGAYRRVPGTPEDNWVWEPTGVIDMHRPERWGVLQFSDHMTDLPS
                     LRPLEEWDERLTLIDLLEAQRAYRLEHGRFASALTDLGFEHPSLSLEATSAQFRATLG
                     SWTIDHEWRLERI"
     gene            3029..3481
                     /gene="mscL"
                     /locus_tag="VFI12_09745"
     CDS             3029..3481
                     /gene="mscL"
                     /locus_tag="VFI12_09745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HET7056734.1"
                     /translation="MFKEFRDFLMRGNVVDLAVAVVIGAAFGAVVTSLVDNIIMPIIG
                     IFGGAPDFSANTFTINGSKFGWGAFLTALIAFIIIAAVIFFVVVKPMNALMARIKRGE
                     VTAAEEILPPTWEYRSASETELNPLGAEGWELVGISPTGFHLKRPLTS"
     gene            complement(3599..>4276)
                     /locus_tag="VFI12_09750"
     CDS             complement(3599..>4276)
                     /locus_tag="VFI12_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056735.1"
                     /translation="TATGVEVTVTEQHPTYFIRVIPGAPTSVTTNAVARANVKKLGAP
                     TDGPYLPCGINTKLSAGGTLSLVTQSGGSGWTINPAAVGKTFNIHGPQIEKCKAKSSR
                     YKGLADTALNKNLQIPGWFSYKEGDTAGFISTDVEGPDGCKAGQEVVNCVVFLPIVVD
                     SPEEQNNDRRQWAVGYAPFYITANNSGNEHYGKLLADYLVYGKGQDGSWGWQQDYEGP
                     ITIRLTK"
BASE COUNT          742 a         1376 c         1374 g          784 t
ORIGIN      
        1 ccaattcgat acgcagaact gcgaaacgga ccgcgcggaa gttcttcccc cgaccgaacg
       61 gggggaagcc agatgggggg catgccccgc tgtagccgcg ggccacgata gtcctcgttt
      121 gagacccggt tcgaggaccg gaaacggtcc gcccgggaac gatcatcgcc ccatgggcga
      181 ttgcggggcc ccgtatccct ccgctacgtt tgggaacgcg cgactaccac gcgacgatgc
      241 cccgggcaag atcccccggg gcatcgcaat tcatccctga tctgcggtta cgccatccgc
      301 cgccgcatcg cgaacccggc gatgccgatg gtgagacccg caagcacgat gtatgccgcc
      361 aggtccaggc cgctgcccgg aagtccgccg gatccggtgt tcggcagctg ctgcgtcgtg
      421 ccgccgccgc tggtgccacc acccgagttg ccgccggtgt tcccgccgcc gccaccaccg
      481 gccgcctgga cgacgatcgt gccggtcatg ttcgggtgga tcgcgcagtg atacgtgaat
      541 gtgccggggt cgttgaacgt gatcgtcgcg ctgctgccgg ctgcgatgtc gccggtgtcg
      601 aacgagccat catcacccgt ggcggtatgg gtgacagaat ccatattcgt ccaggtcacc
      661 gaggacccgg ccgtgatcgt gaccgagttc ggcgagaagg cgaagttctg gatggtgacg
      721 ctcgtcgagt cctgggcaac cgccgggctg ccgccgaagg tgacgagcgc cgcaaacgcc
      781 agcatgatgg cgagcgccgt cgccgtcgca aacattccca aacgtgtcgt gaggatccgc
      841 attgtcgatc cctccatgac aaaggccggt gccggccgca accggtccgg gaccgtggtg
      901 tccgcgccgg tagtgtctct acgtggggga gggggagctg gatgcgcggg ctggggtcac
      961 gaatcaacga gtggcgcgtc gcgaaacctg cggcgtgggt cgcccgtgaa ggcgcgcgcg
     1021 aacagtccag cgcaggagtt gaaacggagc gcgaatggcg gcaggcaggt gtgactgtcg
     1081 tctcgtcttg acgacgatca ggtgtccgct ctcgattgct tcctgccacg attgggcagt
     1141 gaagccgggg ggaatctcac tatcctgttt catgcccttt cacaccgggc gaccgtcgaa
     1201 ccgcatcgtg aaagcatctg tcgtatatac ggctaaaggt atggcacaga tggcctatgc
     1261 ggagtaaccc aaaggtcagg gatcgggatc agtgtgtgcg gtcgattgcg accccgccgc
     1321 cccggctcca ctccagcatc gcggtagcga gcggcatttc ggcggtccct tcgctggcga
     1381 tcggcaggtc ttgcacgtgc gcgctcagtt cctggaaaaa gcggcgcaca tctggattgg
     1441 tcaggaacgc gcggaatcgc tcctggaagt cgccgtccgc aaccgtaatc tccagcgcgt
     1501 agtagccgga gccgatgtag gcgacgagcc gctcgatccc gatcccggag tccagcgcct
     1561 ccgccgggaa ccgctcgtgg atatcccgga cttgaacctc gttccccagt tccatcgggg
     1621 agacataaag cactgtctgc atgaccatcc ctccttcccg ccggttatac cgccggcacg
     1681 ccggaggtca cgtgagtgtc cctacattgg cgctcgttga ccgacggcgc cgggccaacc
     1741 cgggattgct atcatgcgac gattttgccg aggggctggg gcatggcggc ggattccgcg
     1801 ctcctctggc tgggcatgca gatatgtggg gagttgagcg tggtcgaaga gcagtccaac
     1861 cggggcgcga gcaagcgccg tgaccatccg cgcgggtacg tctgcccccg cacgaccgtc
     1921 acgccgcggc tcgacggccg catggacgaa gcgatctggg gacatgctcc ctggaccgag
     1981 gagttcgtcg acatcgaggg tgagctcagg cccaatcccc ggtaccgcac gcgcgtccgc
     2041 atgctctggg acgacgattg cctctatatc ggcgcggaga tggaggagcc gcacgtctgg
     2101 ggcacgctca ccgaccatga ctgcgtcatc tacaacgaca acgacttcga agtctttctc
     2161 gaccccaacg gtgcctgcca gcactacgtt gagctcgagc tgaacgcgct caacacgacg
     2221 tgggacctga tgctgacccg gccgtaccgg gttggcggcc gcgcgatcag cggatgggag
     2281 ttccacgggt tgcgcacggc tgtctcgatc gacgggacgc tcaatgaccc gtccgacatc
     2341 gaccggggct ggaaaacgga gatcgccata ccgtggtcat cgctgcgcga cgtgaccgat
     2401 cgcgatctcc cgccgaagag cggcgaccag tggcggatca acttctcgcg cgtcgaatgg
     2461 cagcacgacg tgatcgacgg cgcgtatcgg cgagtccccg gcaccccgga ggacaactgg
     2521 gtctgggagc cgaccggcgt gatcgacatg caccggcccg agcgctgggg cgtcctccag
     2581 ttctctgacc acatgacgga cctgccgtcc ctccgcccgc tcgaggaatg ggacgagcgc
     2641 ctcacgctga tcgacctcct cgaggcgcag cgcgcatatc gcctggagca cggtcgattt
     2701 gcctccgccc tcaccgacct cggattcgag caccccagtc tctccctcga ggcgacgtcc
     2761 gcccagttcc gagccaccct gggatcgtgg accatcgacc acgaatggcg tctggaacgg
     2821 atttgaatgg tgcggttgca atcgcgctga cctgccccgg aattggggca gtgatgggcc
     2881 attccagtca acccaggccg tggacacata ttcgcccccg ggacgggtgt ccgacgatga
     2941 gggccattac cctccatcgc cgcacgatag tctcccaggg tgctaatctg atcgtacgtc
     3001 cgtcggctag acaacaggga ggatgcagat gttcaaggaa tttcgcgatt tcctgatgcg
     3061 gggcaatgtc gtcgatctcg ccgtggccgt cgtcatcggg gccgcgttcg gagccgtggt
     3121 cacgtcgctg gtagacaaca tcatcatgcc gatcatcggc atcttcggcg gcgcgccgga
     3181 cttcagcgcg aacaccttca ccatcaacgg cagcaagttt ggctggggcg cgttcctgac
     3241 cgcgctgatc gcgttcatca tcatcgccgc ggtcatcttc ttcgtcgtcg tcaagccgat
     3301 gaacgctctc atggctcgca tcaagcgggg agaggttacg gcggccgagg agatcctccc
     3361 gccaacctgg gagtaccgca gcgcttcgga gaccgagctc aatcccctcg gcgccgaggg
     3421 atgggagctc gtggggatca gcccaaccgg cttccacctc aagcggccac tcacctcctg
     3481 agcatcgcag cgcctatggg cgaccccggt tccggactgg atacggacgc cggagccggg
     3541 gccgctcatg acttgatccc ggatacggtt ccgctcggcg ctaccgagcg tccagctgct
     3601 acttcgtcag gcggatggta attgggccct cgtagtcttg ctgccaaccc cagctgccat
     3661 cctggccctt gccatagacg aggtagtcgg cgagcagctt gccgtaatgt tcgtttccac
     3721 tgttattggc ggtgatatag aacggggcat agccgactgc ccattgccgt cggtcattgt
     3781 tctgctcttc ggggctatcc accacgatcg gcaggaagac gacgcaattg acgacctctt
     3841 gcccggcctt gcagccgtcg ggaccctcga cgtcggtcga gatgaagccc gccgtatcgc
     3901 cctccttgta cgagaaccag ccggggattt gcaggttctt gttgagcgcg gtatcggcca
     3961 ggcccttgta ccgcgaggac ttggccttgc acttctcaat ttgcgggccg tgaatattga
     4021 aggttttgcc gacggcggcc ggattgatcg tccacccgct gccgccgctt tgcgtcacga
     4081 ggctaagggt gccgccggcg gagagtttcg tattgatgcc gcacggcaag taaggaccgt
     4141 cagtcggcgc acccagcttc ttgacgttcg cccgcgcgac ggcgttcgtg gttacgctcg
     4201 tgggggcgcc gggaattacc cgtatgaagt aggtcgggtg ctgctccgta accgtcactt
     4261 cgacgccggt tgccgt
//
LOCUS       DASSGX010000541         4003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_640711, whole genome shotgun sequence.
ACCESSION   DASSGX010000541 DASSGX010000000
VERSION     DASSGX010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4003
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640711"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VFI12_09755"
     CDS             complement(<1..195)
                     /locus_tag="VFI12_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056736.1"
                     /translation="MLAADMDQVEMQSGTAVLDDNREMDSGAPISISAAVEIFFGDLR
                     LAPRSKKTYRNGIAKFVRHLA"
     gene            complement(292..681)
                     /locus_tag="VFI12_09760"
     CDS             complement(292..681)
                     /locus_tag="VFI12_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056737.1"
                     /translation="MLLIALNRDVFFGVRLRNLAKELGFDLHLAANPEAVGRALRDRH
                     QTTALVIIDMNVLDQEVDWDALAGILGEYPAVPSLGFGSHTDVETRRAAKKAGLSRIV
                     ANSEFHKDAAALIQRYARIDEDEDDDS"
     gene            908..1228
                     /locus_tag="VFI12_09765"
     CDS             908..1228
                     /locus_tag="VFI12_09765"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET7056738.1"
                     /translation="MSGDDLVRILSALGNAQRLRIVAALVPGRNYVSQLARELQISRP
                     LLHLHLQKLEAAGLVMGSLELSPDGKAMKFYEVAPFSIELSPAAIAEAAGTLSKSGSE
                     AESK"
     gene            1246..1518
                     /locus_tag="VFI12_09770"
     CDS             1246..1518
                     /locus_tag="VFI12_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056739.1"
                     /translation="MGWQEAVISAVAIAFLFGGVVVVLVGGMRVAQTKIQSEASTEHT
                     MMVRSLAEESTVAQRNMANELTEVRLAMVDVRDRLTAIERMMADVG"
     gene            1691..3958
                     /locus_tag="VFI12_09775"
     CDS             1691..3958
                     /locus_tag="VFI12_09775"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HET7056740.1"
                     /translation="MGGLSTAGLARITARHPWRTIAVWILILVIGGWQASMVGSRTTS
                     NFEFNTKPESVKGLDLIESRMGGKQPLAETIVVQSDTLTVDDPEFQNAVNAATSAARG
                     MPDVVASAINYYELNQSTDPNAQAQAAGLVSADKHATLIPLTLKITDLPEPAVEESYI
                     SSVESSGTSNVRVLAVGAISSDHTFSKISEEDLRKGEGIGVGAALVVLLVVFGALVAV
                     GVPLVLALLAISIATGIVSVVAAFSTISFFVTSMVSMIGLAVGIDYALFIVERYREER
                     RHGRPKLEAIEVAGGTASKAVLFSGLTVVFALLGLFMIPTTIFRSLGLGAVVVVIVAV
                     LAMLTLIPAMLSLLGDRIDWPRKRHYDDPARIAAQAERDRETIHSGFWGTITRAVMAK
                     PVVSLLLAAGLLVVLAIPYFDLTLGFNGIGTLPKSNIRDGYEIMAKDFQAGRLSPVKI
                     VVDGKKGEADPGIANLLSAVANDPVLGNVEQPTWSQNGDLAVVNINLTGDADSQVARD
                     AVKELRSSIVPAAFAGVPAKVYVSGDPAFNYDFRELINTWTPIVFAFVLMLSFILLTL
                     AFRSIVVPLKAILMNLLGVGAAYGIMVLVFQKGYLHNLLGFEQTPVIEPWLPIFLFCV
                     LFGLSMDYHVFLLSRIREHYDQSHRNRESVAVGLQSTARIITGAALIMVSVFFGFAAG
                     RLVMFQQMGFGLGMAVLIDATIIRSILVPSAMALLGDRNWYMPKWLNWLPDLRVEGTA
                     RPTPGQTPGIQPAGD"
BASE COUNT          706 a         1258 c         1276 g          763 t
ORIGIN      
        1 ggcaaggtgg cgaacgaact tggcgatccc attccggtag gtcttcttgg agcggggcgc
       61 gaggcgaaga tcgccgaaga agatctcaac cgccgccgaa atcgagatcg gcgcgccgga
      121 gtccatctca cggttatcgt caagcactgc cgtgccggac tgcatctcaa cctgatccat
      181 atccgccgcc aacatgttcc tccaccggcg cgcagcgcac cggaaatgac gggatccgcg
      241 gcggggaaag gcgtacgtac actttaggag gcgatgtccc aaccgtcaag ctcaggaatc
      301 gtcgtcctcg tcctcgtcga tccgggcgta gcgctggatc agcgcggccg cgtccttgtg
      361 gaactcgctg ttggccacga tccggcttag cccagccttc ttggctgctc gccgtgtctc
      421 gacatcggtg tgggacccga agccgaggga cggcaccgcc gggtactccc ccaggatgcc
      481 ggccagcgca tcccagtcga cctcctggtc aagcacgttc atgtcgatga tcacgagcgc
      541 ggtcgtctgg tggcggtcgc gcagcgccct cccaaccgct tccgggttcg cggccaagtg
      601 caggtcgaag ccgagctctt tggccaggtt gcgcaaccgc acgccgaaga agacatcccg
      661 gttgagggca atcaacagca tggggtcctg aggcagcgat gttcgataca tagcgcaagt
      721 gtccagcggt cagcggccgg tgtccagtgg gagtcgtcag ccatcggact tcagacgacc
      781 gatcccagaa atgtctcaac tgacgtctga agtccgatgg ctgaagtctt gactcatcca
      841 tggacacctg taatgatata ctgacacgtg tatgagatga gtgtttggag ggtgtgctga
      901 tgtgtcgatg agtggagacg acctggttcg gatcctgagc gcgctcggca atgcccagcg
      961 gctgcggatc gtggcggcgc tggttcccgg ccggaattac gtcagtcagc tggccaggga
     1021 gttgcagatc agccgaccgc tcctccacct gcatttgcag aagctggagg cggcggggtt
     1081 agtcatgggg tcgctcgagc tgtcgccgga tggcaaggcg atgaagtttt acgaagtagc
     1141 gccgttttca atcgagctca gcccggcggc aatcgccgaa gccgccggga ccttgagcaa
     1201 gagcggttcg gaagcggagt cgaaatagaa atggatggaa gcagcatggg ttggcaggag
     1261 gcagttattt cggcggtggc gatcgcgttc ctgttcggag gcgttgtcgt cgtcctggtc
     1321 gggggcatgc gggttgccca gacgaagatc cagtctgagg cgtcgaccga gcacacgatg
     1381 atggttcgca gccttgccga ggagtcgact gtggcacagc gaaacatggc caacgagttg
     1441 accgaggtgc gcctcgcaat ggtcgacgtc cgggatcggc tgaccgccat cgagcggatg
     1501 atggccgacg tcgggtagcg ttcacgattc gttcacgagt cgctattgac gcgagcattt
     1561 ctacactgta gagtcggtct gccgctgatt ctacagtgta gagccgcggc cactccacga
     1621 ggcaattcgg ttcggacaat gacggtccgg ctcgcggcta cacgagcgac cgtccggagg
     1681 tatgcaagca atgggtggac tctcgaccgc aggtctggcg cggataacgg cgcgccaccc
     1741 ctggcgcacg atcgccgtct ggatcctgat cctcgtgatt ggaggctggc aggcgtcgat
     1801 ggtcggcagc cgaaccacct cgaatttcga attcaacacg aaaccggaat cggtcaaggg
     1861 gctggatctg atcgagtccc gtatgggcgg caagcagccg ctggccgaga cgatcgtcgt
     1921 ccagtccgac acgttgacgg tcgacgaccc cgagttccag aacgcggtta acgccgcaac
     1981 ctcggcggcc cgcggaatgc cggacgtggt cgcgagcgcg atcaactact acgagctcaa
     2041 tcaatcgact gatccgaatg cccaggcaca ggcggccggg ctggtgtcag cggacaagca
     2101 cgccacgctg atccccctca cgctcaagat cacggacctt cccgagccgg ctgtcgagga
     2161 gagctacatc tcgtcggttg agtcgagcgg gacctccaat gtgcgcgtgc tggcggtcgg
     2221 cgcgatcagc tctgaccaca ccttctcgaa gatctcggag gaggacctgc gcaagggcga
     2281 aggcatcggc gtgggtgctg ctttggtcgt gctcctggtc gtcttcggcg cattggtcgc
     2341 cgtgggcgtg ccgctggtcc tggcgctgct cgcaatctcg attgcgaccg gcatcgtgag
     2401 cgtcgtcgcc gcattcagca cgatctcctt cttcgttacg agcatggtct cgatgatcgg
     2461 gctcgcggtc ggcatcgact acgccctctt catcgtcgag cgctaccgcg aagagcgccg
     2521 ccacggccgg cccaagctcg aggcgatcga ggtcgccggc ggcacggcca gcaaggcggt
     2581 tctcttctcc ggcctgacgg tcgtgtttgc cctgctcggc ctgttcatga tcccgaccac
     2641 catcttccgc agcctcggcc tcggcgcggt cgtcgtcgtc atcgtggcgg tactcgcaat
     2701 gctgacgttg atcccggcca tgctgtcgct gctcggtgac cggatcgact ggccccgcaa
     2761 gcgccactac gacgatccgg cccggatcgc ggcgcaggcg gaacgggacc gcgagacgat
     2821 ccacagcggc ttctggggca cgatcacccg cgcggtcatg gcgaaaccgg ttgtctcgct
     2881 cctgttggcg gccgggttgc tggtcgtcct cgctattccc tacttcgatc tgacactcgg
     2941 cttcaacggc atcggcacgc tgcccaagag caacatccgc gatggctacg aaatcatggc
     3001 gaaggatttc caggccgggc gcctgagtcc tgtcaagatc gtggttgacg gcaagaaggg
     3061 cgaggccgac ccgggcatcg ccaacctgct ctcagcggtt gcgaacgatc cggtcctcgg
     3121 caacgtggaa caaccgacgt ggagccagaa cggcgacctg gccgtagtca acatcaacct
     3181 gaccggcgac gcggactcgc aggtggcgcg cgatgccgtg aaagagctgc gctcgtcgat
     3241 cgtgcctgca gccttcgccg gcgtgccggc caaggtctac gtttcgggcg atccggcctt
     3301 caactacgac ttccgcgaac taatcaacac atggacaccg atcgtcttcg ccttcgtgct
     3361 catgctcagt ttcatcctgc tgaccctggc cttccgctca atcgtggtgc cgctcaaggc
     3421 gatcctgatg aacctgctgg gcgtcggcgc cgcctacggc atcatggtgc tcgtgttcca
     3481 gaagggatac ctgcacaacc tgctcgggtt cgagcagacg cccgtcattg agccatggct
     3541 gccgatcttc ctcttctgcg tgctcttcgg cctctcgatg gactaccacg tgttcctctt
     3601 gagtcggatc cgggagcact acgaccagtc gcaccgcaat cgggagtcgg tcgcggtcgg
     3661 gctgcagtcg acggctcgga tcatcaccgg cgcggcgctg atcatggtct cggtcttctt
     3721 cggctttgcg gccggtcggt tagtcatgtt ccagcagatg ggcttcggcc tcggtatggc
     3781 ggtcctgatc gacgcgacca tcatccgctc gatcctggtt ccgtcggcga tggcgctgct
     3841 cggcgatcgc aactggtaca tgccgaagtg gcttaactgg ctgcccgacc tccgcgtcga
     3901 gggcacagcc cgcccaaccc ccggccagac tccaggcatc caacccgccg gcgactagct
     3961 ccgccgccac cgatcacgac cgggcgagga gcgtacgctc ctc
//
LOCUS       DASSGX010000542         3586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1513882, whole genome shotgun sequence.
ACCESSION   DASSGX010000542 DASSGX010000000
VERSION     DASSGX010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1513882"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="VFI12_09780"
     CDS             <1..876
                     /locus_tag="VFI12_09780"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HET7056741.1"
                     /translation="SDARSVELIEVGPRDGLQNEARPVSLDDKLALINRLVEAGFRRI
                     EATSFVSPTAIPQLADADDLMPGLPKHLDVDYICLVPNTRGYDRAMVAGVRHVEVFTA
                     ATDTFCQANTRCTIDESFERFAPIMERATVDSVTVRGAVSVAFHCPYSGPVEPEAAVA
                     VAERLLAIGCSEVSIADTIGRASPGEVERLLELALRRLPTDRVAMHFHDTTGQAIENI
                     RVSWDAGISRFDSSVGGLGGCPFAPGAPGNVASESVVHAFEKMNVSTGIDLDSLTATA
                     TWIRNVLGKDEHAPH"
     gene            863..1645
                     /locus_tag="VFI12_09785"
     CDS             863..1645
                     /locus_tag="VFI12_09785"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7056742.1"
                     /translation="MRPTDPTYSRYARHYDQIGQRRFGESSARDLLRLFALQGDHPSS
                     VLDLACGTGAATITFARHGLEVTGLDISRAMLDEAERAAKAAGVAVRWILGDMTAFET
                     EHPYDLCTCFYDAVNYLADLREFTAFARCAFAALAPGGHFAFDINTRRKLAEHWGQMT
                     LIAANDSERFLTYRSWFDERHGVSPLIITGFERRPDGAWDRFDEEHVEVAFAIEDLRD
                     KLESVGFVGTRIIDWREGDISELAPGTEDSFRVLFLAEKPAG"
     gene            1655..1918
                     /locus_tag="VFI12_09790"
     CDS             1655..1918
                     /locus_tag="VFI12_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056743.1"
                     /translation="MTYAIVTAILVLALIAVVIAVVLGTEDEPVDERVEVIVPPPSVP
                     YVGVTPLPPVGVAPIHHEEQQAGERRIVDVPEDADRDSRPIGI"
     gene            1854..2918
                     /locus_tag="VFI12_09795"
     CDS             1854..2918
                     /locus_tag="VFI12_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET7056744.1"
                     /translation="MRDESWTFLRTLIETPGPSGYEREVAAVWRAEAESFADDVDRDL
                     TGNSFAWLRSNPDAPTVLVEGHIDEIGVQITHVDPEGYIWFDEIGGWDAQVLTGQRIR
                     FITGSDVPVIGVVGRKAAHLLKSGDREKAVKTEDLWIDIGAGSREEALKRVSIGDAGV
                     VDAPFTAIGDGLIVSRAIDNRSGAFVALETLRLLAEDRPYANVVALAAAQEEISLAGA
                     FTAAFKTEPMVAIAIDVTHATDYPGADKKRNDEVALGSGPVLTRGSSANPVVYERLVA
                     AAQSAGVAYTVQGSARATWTDADAMIRSGHGPATGVVSIPTRYMHSPNETIAVKDLDA
                     CAQLIAEFIRQIDADADFRP"
     gene            complement(2934..>3586)
                     /locus_tag="VFI12_09800"
     CDS             complement(2934..>3586)
                     /locus_tag="VFI12_09800"
                     /inference="COORDINATES: protein motif:HMM:NF018533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HET7056745.1"
                     /translation="FHLKLSGQISLQRGAERRMAGHPVPDPAGPYPHKATHLSFTFDT
                     GWVLHLSDVRQFGWIRIMPADAVDSFIAAQKFGPEAVGPVGITADQLAANLARRSIPV
                     KLALLDQRVVAGIGNIYVDEALHRAKIHPMTPARDVPPQRIPDLVAAIRWALETGIEQ
                     GGAKIIHNRAYPIDGFPEVHARKGEACPVCGDEIIKIKVGQRGTYLCPTCQPGPPV"
BASE COUNT          632 a         1150 c         1168 g          636 t
ORIGIN      
        1 tctgacgcgc gttcggtcga gttgatcgag gtcggaccgc gggatggcct ccagaacgaa
       61 gcgcgacccg tttccctcga cgacaagctg gcgctgatca accggctcgt cgaggccggg
      121 tttcggcgaa tcgaggccac cagcttcgtg agtccgacgg cgattccaca gcttgccgac
      181 gccgacgacc tcatgcccgg cttgccaaaa catctggatg tcgattacat ctgcctcgtc
      241 ccaaacacgc ggggatatga ccgcgcgatg gtagccggcg tgcgccacgt cgaagtcttt
      301 accgccgcga ccgacacctt ctgccaggcc aacacgcgct gcacgatcga cgaatctttc
      361 gagcgattcg ccccgatcat ggaacgcgcg actgtcgaca gcgtcaccgt acgcggagca
      421 gtctccgtgg cattccattg cccgtacagc ggcccggttg agccagaggc tgccgtcgcc
      481 gtcgcggaac gattgctcgc gatcggctgc tccgaggttt cgattgcgga cacgatcgga
      541 cgcgcttcac ccggtgaggt agagcggctg ctcgaattgg ccctgcgccg gctgccgacg
      601 gaccgggtcg cgatgcattt ccacgacacc acggggcagg ctatcgagaa catccgtgtg
      661 agttgggatg ccgggatatc gcgattcgac tcatcggtgg gcggtctcgg cggctgcccg
      721 tttgcgcccg gcgcaccggg caatgtcgcg agcgaatcgg tggtgcacgc gttcgagaag
      781 atgaacgtct cgaccggaat cgacctcgat tcgctgacgg cgaccgcgac ctggattcgc
      841 aacgtcctcg gcaaagacga gcatgcgccc cactgaccca acctactccc ggtacgcgcg
      901 gcattacgac cagatcgggc aacgccgctt cggcgagagc tcagcgcgtg acctcctgcg
      961 actcttcgcg ctgcaaggcg accatccgtc ctcggtcctc gaccttgcct gtggcactgg
     1021 cgcggcgacg atcacattcg cccgacacgg gctcgaagtc accggactgg acatctctcg
     1081 ggccatgctg gatgaggcgg agcgcgccgc caaagcggcc ggagtcgcgg tcaggtggat
     1141 tctcggggac atgaccgcat tcgagacgga gcacccctac gatctttgca cgtgcttcta
     1201 cgacgccgtc aactacctgg ccgacctccg ggagttcacc gctttcgccc ggtgcgcctt
     1261 tgcggcgctg gcacccggcg gccacttcgc cttcgacatc aacacgcgcc gcaagctggc
     1321 cgagcactgg ggtcagatga ccctgatcgc cgcgaatgac agcgagcgct tcctgacgta
     1381 tcgaagctgg ttcgacgagc gccatggcgt atcgccgctg atcatcaccg ggttcgaacg
     1441 gcgtccggat ggcgcctggg accgcttcga tgaagagcac gtcgaggtcg cgttcgcgat
     1501 cgaggatctg cgcgacaagc tggagtcggt cggatttgtg ggaacccgga tcatcgactg
     1561 gcgtgagggc gatatctccg agttggctcc cggtaccgag gactcctttc gggtactgtt
     1621 tctggctgag aagccggcgg gttaggaggg agacgtgacg tacgccatcg tgaccgccat
     1681 tctggtcctg gccctgatag cagtcgttat cgcggtcgtt ctcggcacgg aggacgaacc
     1741 ggttgacgag cgcgtcgagg tcatcgttcc gcccccgtcc gtgccgtacg tcggcgtaac
     1801 gccacttccg cccgtgggtg tggccccgat ccaccatgag gagcagcagg caggtgagag
     1861 acgaatcgtg gacgttcctg aggacgctga tcgagactcc cggcccatcg ggatatgagc
     1921 gcgaggtcgc ggcggtttgg cgcgccgaag ccgagtcgtt cgcggatgac gttgaccgcg
     1981 atctgaccgg aaactcgttc gcctggctcc ggtccaatcc ggacgcaccg accgtgctgg
     2041 tcgaaggcca catcgatgaa attggtgtcc agattacgca cgtcgatccc gagggctaca
     2101 tctggtttga cgagattggc gggtgggacg cgcaagtctt gaccggtcag cgcatccgct
     2161 tcataaccgg ctcggatgta ccggtgatcg gcgtggtcgg ccgcaaggcg gcgcaccttc
     2221 tgaaatcagg agaccgcgaa aaggcggtca agactgaaga cctctggata gacatcggag
     2281 cgggatcgcg cgaggaagcg ctgaagcggg tctcgatcgg agacgccggg gtcgtggatg
     2341 cgcccttcac cgcaatcggc gatggcttga tcgtctcccg ggcgatcgat aaccggagcg
     2401 gcgccttcgt cgcgctcgag acgctgcgat tgctcgccga ggatcgcccg tacgccaatg
     2461 tggtcgcgct cgccgccgcc caggaggaga tctccctcgc cggcgccttc actgccgcat
     2521 tcaagaccga gccgatggtt gcgatcgcga tcgacgtcac ccacgcaacg gactatcccg
     2581 gcgcggacaa gaagcggaac gacgaggtcg cgctcggcag tggtccagtg ctcacacgcg
     2641 ggtcatcggc caatccagtc gtctacgagc gactggtcgc cgccgcgcaa tcggccggag
     2701 tcgcctacac ggtgcagggc tcggcgcggg cgacgtggac cgacgccgac gccatgattc
     2761 ggtccggtca cggtcccgcg accggtgtcg tctcgatccc gacacgctac atgcactcgc
     2821 caaacgagac gatcgccgtc aaggatctgg acgcctgcgc gcaactgatc gcggagttca
     2881 tccggcaaat cgacgccgac gccgatttcc ggccctgacg cggagcgtca agcctagacg
     2941 ggcggccctg gctggcaagt cgggcagagg taggtgccgc gctgaccgac cttgatcttg
     3001 atgatctcgt cgccgcagac cgggcaggcc tcgcctttgc gcgcgtgaac ttcagggaag
     3061 ccatcgatcg ggtacgccct gttgtgaatg atcttcgcgc caccctgctc gatgcctgtt
     3121 tcgagcgccc accggattgc cgctaccagg tccggaatac gctgtggtgg cacatcccgg
     3181 gccggtgtca tcgggtgaat tttggcccga tggagcgcct cgtccacgta gatgttgccg
     3241 attcccgcaa cgacccgttg atcgagtagt gccagtttca ccgggatcga ccggcgggca
     3301 aggttcgcag ccagctgatc cgcagtaatg cccaccggcc cgactgcttc cgggccgaat
     3361 ttctgggcgg cgatgaagga gtcaaccgcg tccgcaggca tgatccgaat ccagccaaac
     3421 tggcggacgt cgctcagatg cagcacccag ccggtatcga acgtgaacga aaggtgggtc
     3481 gccttgtgtg gatacggtcc ggcgggatcc gggaccggat gcccggccat ccgccgctcg
     3541 gcgccgcgct gcaggctgat ttggccagag agtttcaggt ggaaca
//
LOCUS       DASSGX010000543         2736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1186464, whole genome shotgun sequence.
ACCESSION   DASSGX010000543 DASSGX010000000
VERSION     DASSGX010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186464"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VFI12_09805"
     CDS             <1..835
                     /locus_tag="VFI12_09805"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7056746.1"
                     /translation="SASIQVVERGSRLIGAPLGGVLIAIFGPAGALWIDALTFAVSAG
                     LVYVAIPVAARPPREEPSEGYVADLKAGLAFIRRDRLIFAVVGTVMVTNLLDAIFTVC
                     LPFLAKEVYDSSIALGLMAAASGGGAVVGAIAYGTVGRKVSRRAVFIPAFAIAGIRGL
                     PLALLPAVPVAIAVQAVVGLAAGPLNPILSAVEYERIPVHMRGRVFGAIQAGAWLAMP
                     LGVLMGGFLIEQIGLPRTFLVTSICYLATTLTMVFNPAIRDMDRRPEPVDLAAETAIH
                     A"
     gene            939..1817
                     /locus_tag="VFI12_09810"
     CDS             939..1817
                     /locus_tag="VFI12_09810"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7056747.1"
                     /translation="MSAQSRSQEQAAGLAGVPKEWAEDHHATIFSRWHPFLAPQLRES
                     EALVIAESGAAAGDMVMDVACGAGVPTLRLAETVGRDGRVVAVDPSPVFLAAAAENAR
                     ARGLANIEFIQSSAATLPFDRATFDAGTCHFGAMFFTDLNAGLTRIREVLKPDARAAF
                     VAWGPDEDNELFGAFWSVARPYFPPPPAPNPAIPEADIPKPNRFALAGSLSRALTAAE
                     YRDVRERSEQVNLIWPGHASSCFDFWRQLTKADEQVPAERHAALEEDVLASLGQYAER
                     DTLHFRANVVVTSGAA"
     gene            1834..2607
                     /locus_tag="VFI12_09815"
     CDS             1834..2607
                     /locus_tag="VFI12_09815"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7056748.1"
                     /translation="MAQATGRETLVEPSARNLARFFTGFPRRVMRWDDVWAGDSESPE
                     PIINSAALLRPLDDTSAPELTHRLAHYYSAARGGPWMLWSAWPTPDLAPFGFTLIGRP
                     PLMTRPAGTPPIPQPNGLRIEEVGDAEGLAVYERTLIDGYGLQETMGAVPPGQSLPPE
                     SIPGPVRYWIGYVDGAPAAVAAGVAGDGVVGIYGVATMPDARGKGYGGAVTDAAARCD
                     PSLPAVLQASDMGRPVYERLGFTTIAPYTLWLRTRVANH"
BASE COUNT          393 a          957 c          916 g          470 t
ORIGIN      
        1 cagcgcctcg atccaggtcg tcgagcgcgg atcccgcctg atcggcgcgc cgctgggcgg
       61 cgtcctgatc gcgatcttcg gcccggccgg cgcgctctgg atcgacgcgc tgaccttcgc
      121 cgtctcggcc gggctggtgt atgtcgccat ccccgtcgcc gcccgcccgc cccgggagga
      181 accatccgag gggtacgtcg ccgatctcaa ggccggcctc gcttttatcc gccgggacag
      241 gctgatcttc gcggttgtcg ggaccgtgat ggtcacgaac ctgctcgacg cgatcttcac
      301 cgtctgcctg cccttcctgg ccaaggaggt gtacgacagc tcgatcgcgc tcggcctgat
      361 ggcggcggct tcgggcggcg gcgcggtggt gggcgcgatc gcctacggaa cggtggggcg
      421 gaaggtgtcc cgccgggccg tgttcatacc cgccttcgcc atcgccggga tccggggatt
      481 gccgctggcc ctgcttcctg ccgtgccggt ggcgatcgcg gttcaggcgg tggttggcct
      541 ggccgcgggc ccgctgaacc cgatcctgag cgcagtcgag tacgagcgga ttcccgtgca
      601 tatgcgcggc cgcgtcttcg gggcgatcca ggccggcgca tggctggcga tgccgctggg
      661 cgtgcttatg ggcggcttcc tcatcgagca gatcggcctg cccaggacct tcctcgtcac
      721 cagcatctgc tacctggcca cgaccctgac catggtgttc aatccggcca tccgcgacat
      781 ggatcgtcgt ccggagccgg ttgatctcgc ggccgaaacc gctatccacg cgtgatacag
      841 tagccggacc aaccgcttag cgagacaaat ggatcatccg catcggaatg acggtacgac
      901 tgagtcataa cttctgaatc gcatcaggag gtatcgcaat gtctgcgcag tccagaagcc
      961 aggagcaagc ggcggggctc gccggagtcc cgaaagaatg ggcggaggat caccacgcca
     1021 ccatcttttc ccgctggcat ccattcctcg cgccgcaact ccgggaatcg gaggcgctcg
     1081 tgatcgccga gtccggggcg gcggcggggg acatggtgat ggatgtcgcc tgcggcgccg
     1141 gggttccgac gctgcggttg gccgaaaccg tcggccggga tggccgcgtc gtggcggtcg
     1201 atccgtcacc ggtcttcctg gcggcggccg ccgagaacgc ccgggcgcgg ggcttggcca
     1261 atatcgagtt catccagtcg agcgcggcca cgctgccctt cgaccgcgcg acgtttgacg
     1321 ctggcacctg ccacttcggc gcgatgttct tcaccgatct caacgccggc ctgacccgga
     1381 ttcgcgaggt gctcaagcct gatgcccgcg cggccttcgt ggcctggggc ccggatgagg
     1441 acaacgagct gttcggcgca ttctggagcg ttgcccggcc ctacttccca ccaccgcctg
     1501 cgccgaatcc cgcgattccc gaggcggaca tcccgaagcc gaaccggttc gccctggccg
     1561 gctcgctctc ccgcgcattg accgcggctg agtaccgcga cgtgcgggaa cggtcggagc
     1621 aggtcaacct catctggccg ggccacgcat cgagctgctt cgacttctgg aggcagctca
     1681 ccaaagcgga cgagcaggtg ccggccgaac gccacgcggc cctcgaggag gacgtgcttg
     1741 cctcgctcgg ccagtacgcg gagcgagaca cgcttcattt ccgggccaac gtcgtcgtca
     1801 cctcgggcgc ggcgtaaacg gggaggattc ggcatggccc aggcgacagg acgggagaca
     1861 ctggtcgagc cctcggcccg caacctggcc cggttcttca ccgggttccc gcggcgagta
     1921 atgcgctggg acgatgtctg ggcgggcgat tccgaatcgc cggagccgat catcaatagc
     1981 gccgccctcc tccgccccct cgacgatacc tcggcgccgg agcttacgca ccggctcgcc
     2041 cactattaca gcgcggcacg tggcggtccg tggatgctct ggagcgcctg gccgacgccc
     2101 gatctcgccc cgttcggatt caccctgatc ggtcgtccgc cgctgatgac ccgcccggcc
     2161 ggcacgccgc cgatcccgca gccgaacggg ttgcggatcg aggaggtcgg cgatgccgag
     2221 ggcctcgcgg tctatgagcg caccttgatc gacgggtacg gactccagga gacgatgggc
     2281 gccgtgccgc cgggtcagtc gctgccgccg gagagcatcc ccggtcccgt gcgctactgg
     2341 atcggctatg tggacggcgc cccggcggcc gtggcggcgg gagttgctgg cgatggtgtg
     2401 gtcggcatct atggcgtcgc cactatgccc gacgcgcggg gcaagggcta tggcggcgcg
     2461 gtcacggacg ctgccgcccg ctgcgatccg tccctgccgg ccgtgttgca agccagcgac
     2521 atgggccgcc ccgtctacga gcgcctcggt ttcaccacca tcgcccccta caccctctgg
     2581 ctccgcaccc gggttgcaaa ccactgattt cttaacgacc acagcaccgc gacgcagctg
     2641 ccgctgattt ccgggcggct gccgctgcgt ccttcttgcc cgccaaatat tgggacccgg
     2701 gtacttgtca ataaggtgcg aaccctctcc cagttc
//
LOCUS       DASSGX010000544         3610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1186540, whole genome shotgun sequence.
ACCESSION   DASSGX010000544 DASSGX010000000
VERSION     DASSGX010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186540"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..506)
                     /locus_tag="VFI12_09820"
     CDS             complement(42..506)
                     /locus_tag="VFI12_09820"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7056749.1"
                     /translation="MTDDIRLADLSTGNWETVANLEVKPNQLSFVAPNLRTIAEMQFY
                     DNTFLRVIMNGRKPVGLAAYGIDTDDDNWWLFRFMIAGDQQGKGYGRKALELLIDEWR
                     AIPDIDFILLGYYPDNVVAERLYTSLGFVPGEIAPWGERIARLDLASPADPE"
     gene            complement(503..1513)
                     /locus_tag="VFI12_09825"
     CDS             complement(503..1513)
                     /locus_tag="VFI12_09825"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HET7056750.1"
                     /translation="MTNPLRIVTFGEAMIRLTPPGHERLERTTTLNVTAGGSELNVAV
                     TAVCLDHQAAWISALPDSPLGRMIIRAGRSHGVDMNHVPMLPESAGRAGVYFLEEGTD
                     PRPSAVYYDRARSVMAQLLPGQFNWPALLDGADALMVSGITPALGGGARTETSAAMQA
                     AKAAQIPVFFDLNYRSKLWSENEAKACFGRLVGDIDIMFASGGALRTFFGVKGNSEGE
                     SLVAACRDLGLRACVTTLKQGKNSRSQHMAAMGGTADGQVVETDWTDVEIVDRVGGGD
                     AFAGGFIVGYLEQPDNLRYGLELGLAASALKHTMAGDFLAATRSEIEAAITADEGGAL
                     HR"
     gene            1751..2860
                     /gene="ugpC"
                     /locus_tag="VFI12_09830"
     CDS             1751..2860
                     /gene="ugpC"
                     /locus_tag="VFI12_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HET7056751.1"
                     /translation="MARVIFDQVTKRYAGGVEAVKDLTMEIMDNEFLVLVGPSGCGKS
                     TAMRMVAGLEEISEGRIIIGDRVVNDLPPKDRNVAMVFQSYALYPHMSVRDNLAYPLK
                     LAKVPKDERERRVRETAQMLDIDQYLDRKPKALSGGQRQRVALGRAIVRNADLFLMDE
                     PLSNLDAKLRVQTRAELVKLHDRVQTTTIYVTHDQVEAMTMGDRIAVMSLGVLQQLDT
                     PQNLYDRPLNKFVAGFIGSPSMNFVDTTIEQQGGKLYAKAAGFTVRVPDSKANALQGY
                     LGQAITLGIRPEHLVEIHQADASIPEDSLIHATVDVVELLGNEIFVYLTTSAGQVITA
                     RMDPDLRLNRGQQIQVTAPPDRLHFFDPQSEVSIN"
     gene            3044..>3610
                     /locus_tag="VFI12_09835"
     CDS             3044..>3610
                     /locus_tag="VFI12_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056752.1"
                     /translation="MSRRIFVCLALLSAVLTGFFSPLQASAQSGQTGLNGHSYVDPNY
                     GFTVAWDGRTFAAEEITDTGGTPFGVSLVGRGITASVAVGGYGDLQECVLDRFRRLAS
                     TEGVRGIHESRHLNPLEFGRDVTGTVYQYTYDDPRTGTRETYATYFGCEPLLIRGDEQ
                     PDVVLTNDFGTRLADFARLSAEWIPLIDG"
BASE COUNT          659 a         1171 c         1144 g          636 t
ORIGIN      
        1 atggaacggt ccgcgcggcc gtgtcgtgcg ctggacaccg atcactccgg gtcggcgggc
       61 gacgcgagat cgagccgggc gatccgctcg ccccatggcg caatctcgcc gggcacgaaa
      121 ccgaggctgg tgtagagccg ctcagcgacg acgttgtccg ggtagtagcc gagcaggatg
      181 aagtcgatat ccgggatcgc gcgccactcg tcaatcagca gctcaagcgc cttgcgcccg
      241 tagcctttgc cttgctgatc ccccgcgatc atgaaccgga acagccacca gttgtcgtcg
      301 tccgtgtcga tcccgtacgc ggcgagaccg accggcttcc ggccgttcat gattacgcgc
      361 aaaaacgtgt tgtcgtagaa ctgcatctcg gcgatggtgc ggaggttggg cgcgacaaag
      421 ctgagctggt tcggcttgac ctcaagattg gcaaccgtct cccagttacc cgtcgatagg
      481 tcggcgagcc ggatgtcgtc cgtcatcgat ggagtgcccc gccctcgtca gctgtgatgg
      541 ccgcctcgat ttcggaccgg gttgcggcga ggaagtcgcc cgccatggtg tgcttgagcg
      601 cgctcgcggc cagcccgagc tccagtccat atcgcaggtt atcgggctgc tcgaggtagc
      661 cgacgatgaa gccgccggcg aaggcatcgc caccgccgac ccggtcgacg atctcgacat
      721 cggtccagtc ggtctcgacc acctggccat cagcggtccc gcccatcgcc gccatgtgct
      781 gcgagcggct attcttgccc tgcttgagcg tggtcacgca tgcccgcagg ccgagatccc
      841 ggcaggccgc gacgagcgat tcgccttcgc tgttgccctt gacgccgaaa aaggtgcgga
      901 gtgcgccgcc ggacgcgaac atgatgtcga tgtcgccgac gagccggccg aaacaggctt
      961 tcgcttcgtt ctccgaccaa agcttgctgc ggtagttgag gtcgaagaag accgggattt
     1021 gcgccgcttt cgccgcctgc atcgcggcgc tggtctctgt gcgggcgccg cccccaagcg
     1081 ccggcgtgat gccggagacc atcagggcgt ccgcgccatc cagcagcgcc ggccagttga
     1141 attgcccggg cagcaattgc gccatgaccg agcgggcgcg atcgtaatag acggcggacg
     1201 ggcgcggatc ggtcccctcc tccaggaaat agacgcccgc gcgcccagcc gactccggga
     1261 gcatcgggac atgattcatg tccaccccgt gcgaccgtcc cgcccggatg atcatgcgac
     1321 cgagtggcga atcgggcagg gccgatatcc acgccgcctg gtggtcgagg caaacggcgg
     1381 tcacggccac gttcagctcc gagccgccgg ccgtcacgtt caaggtggtc gtccgctcga
     1441 ggcgctcgtg gccgggcggc gtcaatcgga tcatggcttc gccgaacgtc acgatccgca
     1501 gcgggttggt cacgaaaagc actcccaata catcaaacgt cgaaccatgc ccgggattgt
     1561 agtgggcggc gggtgatgcc ggaacggaat gtttgcttga cactgccagt tgtgccccgg
     1621 atactgcgcc cacacgtgtt gtaagacgcc gcaaccgggt tcgccggggt cgaaggtgac
     1681 gttggccgaa gcagagggcg tctcttaact gccgcagggc ggttctgtaa tccaagggag
     1741 gacgcggacg atggcgagag tgatttttga ccaggtcacc aagcgctacg cgggcggtgt
     1801 cgaagcggtc aaagacctga ccatggagat catggacaac gagttcctgg tgctcgttgg
     1861 tccatccgga tgcggcaagt cgaccgccat gcggatggtc gccggcctcg aagagatctc
     1921 agaaggccgg atcatcatcg gcgaccgggt cgtcaacgac ttgccgccga aagatcgcaa
     1981 tgtcgcaatg gtgttccaga gctacgcgct ctacccgcac atgtcggtgc gcgacaacct
     2041 cgcctatccg ctcaagctgg ccaaggtgcc gaaggatgag cgggaacgac gggttcgcga
     2101 gaccgcgcag atgctcgaca tcgaccagta cctcgaccgc aagccgaagg cgctctccgg
     2161 cggccagaga cagcgcgtcg ccctcggtcg cgcgatcgtc cgcaacgcgg acctcttcct
     2221 catggacgag ccgctctcca accttgacgc caagctccgc gtccagacgc gggccgagct
     2281 ggtcaaactg cacgatcgcg tccagacgac gaccatctac gtgacccacg accaggtcga
     2341 agccatgacg atgggcgacc gtattgcggt catgtccctc ggcgtgctgc agcagcttga
     2401 tacgccgcag aacctctatg accggccgtt gaacaagttc gtcgccggct tcatcggctc
     2461 gccgtcgatg aacttcgtcg acacgacgat cgagcagcag ggtggaaagc tctacgccaa
     2521 ggccgctggc ttcaccgtcc gggtgcccga ttcgaaggcg aacgccctgc agggctacct
     2581 gggccaggcg attacgctcg gtatccggcc ggagcacctc gtcgagatcc accaggcgga
     2641 cgcgagcatc ccggaggact cgctgatcca cgcgacggtc gacgtagtcg aattgctcgg
     2701 caacgagatc ttcgtgtacc tgacgaccag cgcagggcag gtgatcacgg cgcgcatgga
     2761 tccggatctc cgcttgaacc gtgggcagca gattcaggtc acggcgccgc cggatcggct
     2821 ccacttcttc gatccgcagt cggaggtctc gatcaactag cccagccgcg tcttccaccg
     2881 aaatgccggg ccagggcaat agccctggct cattcttaat gcgccaaccc acccgaaatc
     2941 gccggtttcg cgttggcggc atcggccaaa tgtccaattg acaggcaagg gcggagtgct
     3001 aggatcgttc tgcgatccca gacaaacctg gaaggaacgc ggcatgagcc gtcgaatctt
     3061 cgtctgtctc gcgctgctct ccgctgtact cactggattc ttctcgccct tgcaggcatc
     3121 ggctcagagc ggccagacag gcctcaatgg ccactcgtat gtcgacccga actacggctt
     3181 caccgtcgcg tgggacgggc gaacattcgc cgccgaggag attaccgaca ccggtggcac
     3241 gccgttcgga gtctccctgg tcggccgggg cattacggca tcggttgccg tcggcggcta
     3301 cggagacctc caggagtgtg tcctcgatcg atttcgccgg ctggcgagca ctgagggcgt
     3361 gcgtggaatc cacgagtccc gccatctcaa cccactcgag tttggccggg atgtgaccgg
     3421 gacggtctac cagtacacct acgatgatcc ccgcaccgga acacgtgaga cgtatgcgac
     3481 ctacttcggc tgcgagccgc ttctcatacg tggcgacgaa cagccggatg tcgtcctcac
     3541 caatgacttt ggcacgcgcc ttgccgactt tgcgcggttg agcgccgaat ggattccgct
     3601 catcgacggg
//
LOCUS       DASSGX010000545         2040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_641187, whole genome shotgun sequence.
ACCESSION   DASSGX010000545 DASSGX010000000
VERSION     DASSGX010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641187"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="VFI12_09840"
     CDS             <1..695
                     /locus_tag="VFI12_09840"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HET7056753.1"
                     /translation="ATFLVFSDYMRPAIRLSALSGYKSTFVFTHDSIAVGEDGPTHEP
                     IEQTMSLRLIPKLVVIRPADGNETAEAWRITLEHQGPTCLILSRQDLPVLDMSGAKGS
                     LKQGAYILRDSDGKPDIVLIGTGSEVQLAVGAAATLAKQGIKARVVSMPSWELFDDQT
                     ADYRQSVLGGHDVKRLSVEAGTTIGWQRFADASVGIDRFGESGPGQQVLDHFGFTVDN
                     VVEQALALLGKK"
     gene            713..1846
                     /gene="tal"
                     /locus_tag="VFI12_09845"
     CDS             713..1846
                     /gene="tal"
                     /locus_tag="VFI12_09845"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HET7056754.1"
                     /translation="MTNPKVQQLCDLGQSIWQDDIARSMLTTGKLQQTIDEIGVRGLT
                     SNPTIFEKAISGGNDYDAQITELLGEGKAAQDIFEALEVSDLQGALDLFRPIYDETNG
                     GDGFCSIEVFPDAARNPDATREQTRRLWNEVNRPNLMVKIPGTIEGSPVVAEMIAEGI
                     NINITLLFSIASYERVAWAYIEGLEKRLASGEPIDHVASVASFFVSRVDTLVDKKLDE
                     KAAADPDNAAKYEALKGKIAVANAKLAYARYQEIFSGDRFLKLKAEGAKPQRPLWAST
                     GTKNPAYSDTLYVDTLIGPDTVNTMPEKTIAAFLDHGTVKRTVDTDVDLARQQMKQLA
                     ELGIDIDAVTHQLEEDGIASFIKSFESLLAGVEAKRSQLAGVR"
BASE COUNT          404 a          662 c          650 g          324 t
ORIGIN      
        1 gcgcgacctt cctggtcttc tccgactaca tgcgccctgc catccggctc tccgcgctct
       61 cggggtacaa gtcgaccttc gtcttcaccc atgacagcat cgccgtcggc gaagacgggc
      121 cgacccacga gccgatcgag cagacgatgt cgctccggct gatcccgaag ttggtcgtga
      181 tccggccggc cgacgggaat gagacggccg aggcctggcg catcacgctc gagcaccagg
      241 ggccgacctg cctgatcctc tcccgtcagg acttgccggt cctcgacatg tccggcgcga
      301 agggatcgct caagcagggc gcctacatcc tgcgcgacag tgacggcaag cccgacatcg
      361 tcctgatcgg caccggctcc gaagtccagc tggcggtcgg cgcggccgca acgctggcca
      421 agcagggaat caaggctcgc gtcgtcagca tgccgtcgtg ggagttgttc gacgaccaga
      481 ctgccgacta ccggcaatcc gtgctcggcg gccatgacgt taagcggttg tcagtcgagg
      541 ccgggacgac gatcggctgg cagcgcttcg ccgacgccag tgtcgggatc gaccgcttcg
      601 gcgagtccgg tcccggccag caggtcctcg accacttcgg attcaccgtc gacaacgtgg
      661 tggagcaggc attggcgctg ctcggcaaga aataatgcga ggagcatcca aaatgactaa
      721 tcccaaggtc caacagctct gtgatctcgg ccaaagcatc tggcaggacg atatcgcccg
      781 ctcgatgctg accactggca agctgcagca gacgatcgat gagatcggcg tccgcggtct
      841 gacctcaaac ccgacgatct tcgagaaggc gatctccggc gggaacgact acgacgcgca
      901 gatcacggag ctgctcggcg aaggcaaggc ggcccaggac atcttcgaag cgctcgaggt
      961 ctccgacctg cagggcgcgc ttgacctctt ccgcccgatc tacgacgaaa ccaacggcgg
     1021 cgatggattc tgctcgatcg aggtcttccc tgacgccgcg cgcaaccccg acgccacccg
     1081 ggagcagacc cggcggcttt ggaacgaggt caaccggccg aacctgatgg tcaagatccc
     1141 gggcacgatc gaaggctctc cggtcgtcgc cgagatgatc gccgagggca tcaacatcaa
     1201 tatcacgctg ctcttctcga tcgcctccta cgagcgcgtc gcctgggcat acatcgaggg
     1261 gctcgagaag cggctcgcga gtggcgaacc aatcgatcac gtcgcgtcgg tcgcctcctt
     1321 tttcgtcagc cgcgtcgaca cgctcgtcga caagaagctg gatgagaagg cggccgccga
     1381 tccggacaac gccgcgaagt acgaggcgct caagggcaag atcgccgtgg cgaacgccaa
     1441 gctcgcctat gccaggtacc aggagatctt cagcggcgac cggttcctga agctcaaggc
     1501 ggaaggggcg aaaccccagc ggccgctctg ggccagcacc ggcaccaaga acccggccta
     1561 ctccgacacg ctctacgtcg acacactgat cggcccggac acggtcaata cgatgcccga
     1621 gaagacgatc gctgccttcc tcgatcacgg cacggtcaag cggacagtcg acacggatgt
     1681 cgacctcgcg cggcagcaga tgaagcagct ggcggagctg ggcatcgata tcgacgcggt
     1741 cacccatcag ctcgaggagg atggcatcgc ctccttcatc aagtcgttcg aatcgctgct
     1801 cgccggagtc gaggcgaagc ggtcgcaact ggccggcgtc cggtaatcaa cgggaaggcg
     1861 aggattgatg tctgacggcg gcgatgcggc acgacacaca ggtgtcgttg gcctggcggt
     1921 gatgggcgag aacctggcgc tcaatatcga ggagaagggg tttccgatcg cggtgttcaa
     1981 ccggaccgtc gcgaaggtgg acgacttcgt ggcgcggcac ccgggcaagc agcttgtcgg
//
LOCUS       DASSGX010000546         3272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_969953, whole genome shotgun sequence.
ACCESSION   DASSGX010000546 DASSGX010000000
VERSION     DASSGX010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3272
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969953"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VFI12_09850"
     CDS             <1..622
                     /locus_tag="VFI12_09850"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HET7056755.1"
                     /translation="LSCGPCPAATTDYLNLRSAPNLSSTVLLVIPPWQEVAYDASAGP
                     VNGYYAVTYAGVSGYAHGLYLLRFPAFATPTDWLNLRSDPSLNSAVLQVMAPGSNVEV
                     LGLGENGFYSVRWEQRLIGFASSDYLSLDHGAGFANGDTVVVRTDALNVRARGGLSYA
                     IQDTIFSGRDVTITGGPVERDGYSWYRVDAPHLDSGWVAGEFLART"
     gene            complement(771..1184)
                     /locus_tag="VFI12_09855"
     CDS             complement(771..1184)
                     /locus_tag="VFI12_09855"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HET7056756.1"
                     /translation="MHAQRMRGKQVTRQAENKRNAMAFYDLMFNRSKPREAVERYVGT
                     SYTQHNPGVGDGADAFIAYFERMAREYPGKWVAFQRAIAEDKYVVLHCHQSWPGDHDY
                     AGIDIFRFDDSGKIVEHWDVLQIIPETSANANTMF"
     gene            complement(1279..1494)
                     /locus_tag="VFI12_09860"
     CDS             complement(1279..1494)
                     /locus_tag="VFI12_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HET7056757.1"
                     /translation="MSQPPPGRDWTLHLVVVAVVLALVAAFVAQNYESVEVRLLFWRF
                     NMRAAWIGTSALLIGIVIGRFMPRRRD"
     gene            complement(1677..2084)
                     /locus_tag="VFI12_09865"
     CDS             complement(1677..2084)
                     /locus_tag="VFI12_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056758.1"
                     /translation="MAQLRPRRKAFGGRLRCAGYWVRSAETCVFLHRIDRVVDSGRYL
                     VPLERWESVKGGTPMASEIPFQAFIQDVADKLEPYGIDVELITRRQDRPGLLSCRRLD
                     SGRTVFLLADPADCSHARAAVLADFVRQEIPHV"
     gene            complement(2110..2799)
                     /gene="nth"
                     /locus_tag="VFI12_09870"
     CDS             complement(2110..2799)
                     /gene="nth"
                     /locus_tag="VFI12_09870"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HET7056759.1"
                     /translation="MDVASLPHPPPITDIHEAMRRVTEAVRDMPKPSMFQLASEGFGT
                     PFAQLVACIISIRTLEETSLPVSRRLFAVARTAEQMAALPIAELERLITPSTFAERKA
                     AQIHDIAARVLAEYSGELPCDGAVMQSFSGVGPKCANLCLGIACDRPTIPVDIHVHRV
                     VNRWGYVHTKTPEQTLAALEPKLPMEYRPSINRILLPFGKYICTGGTPRCLSCPLIDI
                     CPKVGVTKARK"
     gene            complement(2967..>3272)
                     /locus_tag="VFI12_09875"
     CDS             complement(2967..>3272)
                     /locus_tag="VFI12_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056760.1"
                     /translation="WFVLGSHNLIEVHGVVGNLTFILVLLLVVGAWLGRQAGVMTNTE
                     LVLSVALVILTAAQLGLGYSGRDSGTAASLHIPNGILVTGLTAALIAISFMRQPRDG"
BASE COUNT          563 a         1095 c         1050 g          564 t
ORIGIN      
        1 actctcctgc ggcccctgcc cggccgcgac gaccgactac ctgaacctgc ggtccgcgcc
       61 gaacctgagc tcaaccgtcc tgctggtcat cccgccgtgg caagaagtcg cctacgatgc
      121 ctcggccggc ccggttaacg gttattacgc ggtcacctac gccggcgtct ccggctacgc
      181 ccacggcctc tacctgctcc gcttccccgc gttcgccacg ccgaccgact ggctcaatct
      241 ccgctccgac ccaagcctga acagcgcggt gctccaggtc atggcgcccg gcagcaatgt
      301 cgaggtgctc ggcctcggtg agaacggctt ttactccgtc cgctgggagc agcggctgat
      361 cggctttgcc tcatcggatt acctgagcct tgatcacggc gccggcttcg cgaatggcga
      421 cacggtggtg gtccggaccg acgccctcaa cgtgcgcgct cgcggcgggt tgagctacgc
      481 gatccaggac acgatctttt ccggccgcga cgtgacgatc acgggcgggc cggtcgagcg
      541 ggacggctat tcctggtatc gggtcgacgc gccgcatctc gatagcggct gggtcgccgg
      601 cgaattcctc gcccgcacct gacggcgctg cgaccttaga gcggtgcccg gaagaagtgc
      661 cggttcacaa aacgtagccc cgggcggcct cgcccggccg tgtcacacca aacccgggcg
      721 agccgcccgg ggctacattg ggcaggcagg cacttcttct gaaacccgct ttagaacatg
      781 gtattggcgt tggcagacgt ctcggggatg atctggagga catcccagtg ctcgacaatc
      841 ttgccgctgt cgtcgaagcg gaagatgtcg attccggcgt agtcatgatc gcccggccag
      901 gactgatggc agtggaggac gacgtacttg tcctccgcga tcgcgcgctg aaacgcgacc
      961 catttgccgg gatattcccg cgccatccgc tcgaagtagg cgatgaaggc atcggctccg
     1021 tcgccaacgc ctgggttgtg ctgggtgtag gacgtgccga cataccgctc gaccgcctcg
     1081 cgtggcttcg accggttgaa catgaggtca tagaacgcca tcgcgttccg cttgttctcg
     1141 gcctgccgcg tcacctgctt acctcgcatt cgttgggcat gcatccaggc aaccaccgtc
     1201 gtcccgggcg gcctcgcccg gttcccgtgg agcgcggccg ggtgggccgc ctgggacgac
     1261 gttgaatggg aagatcggtt aatcccggcg gcgcggcatg aatcgaccga taacgatgcc
     1321 gatcaggagg gccgacgtgc caatccaggc agcccgcatg ttgaatcgcc agaacagcag
     1381 gcggacctcc accgactcat agttctgcgc tacgaacgcc gccaccagcg ccagcacaac
     1441 cgctactacc acgaggtgca gcgtccagtc acgtcccggc ggcggttgac tcatgcgtcg
     1501 ctcaatgtgt cagtcggctg cggcgttgcg ccacttcaga gatgaggcgg ccacccgggg
     1561 gtcgaagccc gctgtcggat gccgccgtgc gggatgccgg cgtgtcggca tccccgggtg
     1621 gggcaattta tcgtaacaaa cgccgtgcaa gcgcacagtg ttcaggcaac aaaaggtcag
     1681 acatgcggaa tttcctgcct gacgaagtcc gccagcaccg ccgcacgggc atgtgagcaa
     1741 tccgcgggat ccgccagcaa gaagaccgtg cgacccgaat ccagccgccg gcaggacagc
     1801 aggccgggtc gatcctggcg tcgcgtgatc aactccacat cgatgccgta tggctccagc
     1861 ttgtcagcca cgtcttgaat gaacgcctgg aacggaattt cgctcgccat cggagtgccg
     1921 cctttcacgc tctcccatct ttcgagcggg accagatacc ggcccgagtc caccacccta
     1981 tcgatcctgt ggagaaatac gcaagtttcc gcgctacgca cccagtagcc ggcacaacgt
     2041 aaccggccgc caaacgcttt gcggcgaggc cgaagctggg ccatgacgtc gcggctcgtg
     2101 gcgaaggcat cactttcggg ctttcgtcac gccgactttc gggcaaatgt cgatcagggg
     2161 gcaggagagg cagcggggcg tgccgccggt gcagatgtac ttgccgaacg gcagcaagat
     2221 ccggttgatc gacggtcggt actccatcgg cagcttcggt tcgagcgccg cgagcgtctg
     2281 ctcgggcgtc ttcgtatgca cgtagcccca tcgattgacc acgcgatgga catggatatc
     2341 gaccgggatt gtcggtcgat cgcaggcgat gccgagacag aggttcgcac atttcggacc
     2401 gacgccggag aaggactgca tcaccgcgcc gtcgcagggc agttcgccgc tatattcggc
     2461 cagcacgcgc gctgcaatgt cgtggatctg ggccgccttg cgctcggcga aggtcgatgg
     2521 ggtaatcagc cgctccagct cggcgatcgg cagcgccgcc atctgctccg ccgtccgcgc
     2581 caccgcgaag agccggcgcg agaccggcag gcttgtctct tccagcgtcc ggatcgagat
     2641 gatgcaggcg accagttgcg cgaacggcgt cccgaagccc tcggaggcga gctggaacat
     2701 ggacggcttc ggcatatccc gcaccgcttc cgtcacccgg cgcatcgcct cgtggatatc
     2761 ggtgatcggc ggcggatggg gtagcgacgc aacgtccacg cactcccctc ccatgactcg
     2821 accggcacaa cgccggccat cctaccgccg gccggacctg caccggttcg ctggggctac
     2881 cggctaacgg gcgggccgcc cggcctgctt tggctggcgt attggagttg cccggagctt
     2941 cagcgctggg cggtcccgac gcgcctctat ccgtcccgcg gctggcgcat gaagctgatc
     3001 gcgatcaggg ccgcggtcag gccggtgacc agaattccgt tggggatgtg gagcgaggcc
     3061 gctgtgccgc tgtcccgccc gctgtatccg aggccgagtt gggcggcggt caggatcacc
     3121 agggcgacgc tcaggaccag ctcggtattg gtcatcacgc ccgcttgccg gccgagccat
     3181 gcgccgacca cgagcagcag cacgagaatg aaggtcaggt tgccgacgac cccgtgaacc
     3241 tcgatcaggt tgtgggagcc cagcacgaac ca
//
LOCUS       DASSGX010000547         2143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_424308, whole genome shotgun sequence.
ACCESSION   DASSGX010000547 DASSGX010000000
VERSION     DASSGX010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424308"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..476)
                     /locus_tag="VFI12_09880"
                     /pseudo
     CDS             complement(36..476)
                     /locus_tag="VFI12_09880"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
     gene            complement(473..997)
                     /locus_tag="VFI12_09885"
     CDS             complement(473..997)
                     /locus_tag="VFI12_09885"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HET7056761.1"
                     /translation="MYTVGHSHQEPEAFLALLRQHRIATLIDVRSAPFSRYVPHFNQP
                     VLQGWLDRAGVRYVWLGDSLGGRPQDPACYRGGVVRPGNVDYVAMACQPWYQAGIVRL
                     LAEGERSVAAIMCSEEDPRRCHRHRLIEPSLREHGVIVQHIRGDGTLETIDPGETPAV
                     SAPSPQLALRGFGA"
     gene            1163..1948
                     /locus_tag="VFI12_09890"
     CDS             1163..1948
                     /locus_tag="VFI12_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056762.1"
                     /translation="MPITGWWVRLRQAWKDRPRRRQMVVTDITRMEGDRVCVGGYLDD
                     GVAVRPVAGRAGPNERWLRSARDAPVVPFAVVELRVNRAPKDLRAPHTEDRSISPNGH
                     RVLRTLNPVEQRDLLARFASPSVRAIFGAEVYADSGGQWGRFVRVGEGQRSLGTIPVD
                     HLHAVRYHHYPDRGRWDYRLRFTDGTGEVFQLAVVDLAFRQGLDALRDSGLAPDVAAA
                     TTLAALQRQTVYLRIGLARGWDRHPDRCYLQITGVYGFESGSR"
BASE COUNT          333 a          687 c          754 g          369 t
ORIGIN      
        1 tctcaacctt cgactcacga ctcacgactc acgactcaca ccagatggac gatctccacg
       61 tccggccagg tctgcgccag gtgctcggcg acgaggcggc ggtggcagtg gtccggggtc
      121 ggctcgctgc agagcaggca ggaagcctgg gccgcgaatg cggcgcggtc gaggcgggcc
      181 gggatttcgc gatcgtccat tagcgattcg aagcgggaga cctagcggtc ccagtcgtgg
      241 tcctggcggt agtcgcccag gatttccgcc gtgggagcaa gatcgggcag gtggatgtag
      301 ccgcagccgt tgagtcggtc gaggaaccag gcgaggtcgc tccccttggc gaagccggag
      361 agctgcccgc cggggttgag gcggatatcg acgacgcgct ccacaccatg ctcggcgagg
      421 agcgcgaaga attgcgcggc cgatttccgc gtgaagccga tggtgtaaag cttcatgcgc
      481 caaatcctct caacgcgagt tgcggcgatg gcgcagaaac agcaggtgtc tcgcccggat
      541 cgatggtctc caacgtcccg tctccccgga tgtgctgaac gatgaccccg tgctcgcgca
      601 gcgatggctc gatgaggcgg tggcgatggc agcggcgcgg atcctcctcg ctgcacatga
      661 tcgcggccac actgcgctcg ccctcggcca gcagccgcac gatacccgcc tgataccacg
      721 gttggcacgc catcgccacg taatccacgt ttccgggccg gaccacgcca ccgcggtagc
      781 aggcgggatc ctggggacgg cctccgagcg agtctcccag ccagacatag cggacgccag
      841 cgcgatcgag ccagccctgg aggacaggct ggttgaagtg tgggacgtag cggctgaacg
      901 gggcgctgcg gacgtcgatc agcgtggcga tccggtgctg acgcagcagc gccagaaatg
      961 cctccggctc ctggtggctg tgcccgaccg tgtacatcgt tggcgggtcc gcatggccgt
     1021 tctcacggga gccgtctgca ctgccgggag gggagaacgg cggcggcagc ggcaactgct
     1081 gtacggcatc cgcggcccgc gatagagtgc cgcacgacga tttctcagct ctcttccgtt
     1141 tccgtggcgt gggaggcata ccgtgccgat caccgggtgg tgggtcagac tgcgtcaggc
     1201 gtggaaggat agaccgcggc ggcgccagat ggtcgtcacg gacatcacac gcatggaggg
     1261 ggatcgggtc tgcgtcggcg gctacctcga tgacggcgtt gccgtgcggc ccgtcgcggg
     1321 ccgggcgggg ccgaacgagc gctggctgcg ctcggcgcgc gatgcgccgg tcgtcccctt
     1381 cgccgtcgtc gagctgcgcg tgaaccgagc gccgaaggat ttgcgcgcgc cgcacaccga
     1441 agaccggtct atttctccca atgggcaccg ggtcctgcgt acgctgaacc cagttgaaca
     1501 gcgcgatctg ctcgcacggt tcgcttcgcc atcggtgcgc gctatcttcg gcgccgaggt
     1561 ttacgccgac tcaggcgggc aatggggacg ctttgtccgc gttggcgagg gccagcgctc
     1621 gctcgggacg atcccggtcg accatttgca cgcggttcgc taccaccatt atccggaccg
     1681 cggacgctgg gactatcggc tgaggttcac ggacggcacc ggggaggtgt tccagctcgc
     1741 tgtcgtcgat ctcgcatttc gccaggggct cgatgccctg cgggacagcg gtctggcgcc
     1801 agacgtggcg gcggcgacga cgctcgccgc cttgcaacgc cagaccgtct atctccgcat
     1861 cggtctggcc cgtggttggg accgccaccc ggatcgctgc tatctccaga tcaccggggt
     1921 gtacggattt gagtcgggaa gtcggtgagt cggtgagtcg gtaagttcgg atcgggtgtt
     1981 ggtagacgca gggctaggtt cgccgtgacg cctgcgtcct ccgtgctgcc gctcacccga
     2041 caccccatca cctcgtcaac cgcctccgtc ttctgcggcc gtgatggcgg cgaggatttc
     2101 ctccggcggc ggtgcgccgt gctcagagaa gatgatccgg ccg
//
LOCUS       DASSGX010000548         2204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_532062, whole genome shotgun sequence.
ACCESSION   DASSGX010000548 DASSGX010000000
VERSION     DASSGX010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532062"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VFI12_09895"
     CDS             <1..198
                     /locus_tag="VFI12_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HET7056763.1"
                     /translation="ETAVEAAGAIHTDIQRGFIRAEVVGYDDLITAGSMAETKKHGTF
                     RREGKTYVVQDGDIINFLFNV"
     gene            206..919
                     /locus_tag="VFI12_09900"
     CDS             206..919
                     /locus_tag="VFI12_09900"
                     /inference="COORDINATES: protein motif:HMM:NF025460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4272 domain-containing protein"
                     /protein_id="HET7056764.1"
                     /translation="MADYEDAELDINLQEPDDIGARIVLLLALSIWPEVAETDERNTW
                     QRWLEAQGVMPMATDIEEAVLAARSLSDDALDICERAGDSLPALAWAVALVDEIPLVE
                     PESDRAEMIERIPVPNEGIEPFLDQLVLRDEDEIALERERAEIWNWRLVSEEIRRQSK
                     GANRDEVEEAIREVVLESAISFAIAEIDTEDFLVDGVPVRALAHDLLGALIVTSEERL
                     RAFNWLCGLTEWDVVHLPD"
     gene            complement(884..1582)
                     /locus_tag="VFI12_09905"
     CDS             complement(884..1582)
                     /locus_tag="VFI12_09905"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HET7056765.1"
                     /translation="MSVAPGTVKICSLQQPVHAEYVVEAGADLFGLIFAEARRQVSVP
                     AAREIVGQARTLRADGGPRAVGVFVEQVANDINRIADEVGLDLVQLHRPEAAVGQRIE
                     RPVIVVIHATPQTKVSAVAEYIGAIEESGTLVAGIAVDAYSASSHGGTGKVADWAIAR
                     QLARSYPTLLAGGLHPDNVAGAIQAVSPYGIDVSSGVETDGEKDRAKIIAFAHAARSA
                     FAASVGQVNDVPFG"
     gene            complement(1579..>2204)
                     /locus_tag="VFI12_09910"
     CDS             complement(1579..>2204)
                     /locus_tag="VFI12_09910"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HET7056766.1"
                     /translation="SKGRFPVEMDPAGVAAEYAIGGAAAMSILTDEPFFQGSLADLSA
                     AAEVAHAGPTPMAVLRKDFVIDEYQILEARAAGADAVLLIVAALDQSTLKRLLDFAGA
                     TGLDALVEVHDEDELARAESAGASIIGVNNRDLRNFTVDLAVTERLASRRTTDARLVS
                     ESGIVTRGDVRRLEAAGADAILVGESLVLAPDRSAAIRQLRGQGERE"
BASE COUNT          397 a          715 c          715 g          377 t
ORIGIN      
        1 gagacggcgg tcgaggcggc gggagcgatc cacaccgata tccagcgcgg ttttatccgg
       61 gctgaggtcg tgggttatga cgacctgatc acagccgggt ccatggccga gaccaagaag
      121 cacggcacgt tccggcgcga gggcaagacc tacgtcgtgc aggacggcga tatcatcaat
      181 ttcctgttca atgtctagga tcgatatggc cgactacgaa gacgcggagc tcgatatcaa
      241 cctccaggag ccggacgaca tcggcgcccg gatcgtcctg cttctggccc tctcgatctg
      301 gcccgaggtc gccgaaacgg acgagcgaaa cacctggcag cgctggctgg aggcgcaggg
      361 cgtgatgccg atggcgaccg acatcgagga ggcagtcctc gcggctcgat ccctgtccga
      421 tgatgccctc gatatctgcg aacgcgccgg tgactcgctc ccggcactcg cctgggctgt
      481 cgcgctggtc gacgagatcc cgctggttga gccggaatcg gatcgggcgg agatgattga
      541 gcggattccg gtgccgaatg aggggatcga gccgttcctc gatcaattgg tcctgcggga
      601 cgaggatgag atcgcactgg agcgggagcg ggccgaaatc tggaattggc ggctggtctc
      661 agaggagatc cggcggcaaa gcaagggcgc gaatcgcgac gaggtcgagg aggcgatccg
      721 ggaggtcgtg ctggaaagcg cgatcagttt cgcgattgcg gagatcgaca ccgaggactt
      781 cctggtcgac ggtgtgccgg tccgcgcgct cgcccatgac ctgctcggcg ccctgatcgt
      841 gacgtcagag gagcgtttgc gcgccttcaa ctggctctgc ggcctaaccg aatgggacgt
      901 cgttcacttg cccgactgac gcggcaaacg ccgatcgcgc cgcgtgcgca aaggcgatga
      961 tcttggcccg atccttctcg ccgtcggtct caacaccgct gctgacatca atgccgtacg
     1021 ggctgacggc ctggatcgcc ccggcgacgt tatccgggtg caacccgccg gccaacagcg
     1081 ttgggtacga tcgagccaac tgccgcgcaa tcgcccagtc cgcgaccttg ccggtcccgc
     1141 cgtgtgagct ggcggagtac gcgtcgacgg cgattccggc gaccagcgtc ccagactctt
     1201 cgattgcgcc gatgtactcg gcgaccgcgc ttactttagt ctgcggcgtg gcgtgaatga
     1261 cgacgatgac cggccgctcg atccgctggc ccactgccgc ctccggtcgg tgaagttgga
     1321 ccaggtctaa cccgacctca tccgcaatcc ggttgatgtc gttcgcgacc tgctcgacga
     1381 agaccccaac cgcgcgcggg ccaccgtccg cacggagggt ccgtgcttga ccgacaatct
     1441 cgcgagccgc cggcacggaa acctggcgtc gcgcttcggc gaagatcagg ccgaacaggt
     1501 cggctcctgc ctcaacgacg tactcggcgt gcaccggttg ttggagcgag caaatcttga
     1561 ccgttccagg cgcaacgctc attcccgttc gccctggccg cggagctgtc tgatcgctgc
     1621 tgaacggtcc ggcgcgagaa cgagcgattc gccgaccaga atggcgtcag cgccggccgc
     1681 ttccagccgc ctaacgtcgc cccgggtgac gatgccgctc tccgagacaa ggcgagcgtc
     1741 ggtcgtgcgc cgggacgcaa gtcgttccgt caccgcgagg tcgacagtga agttccgcaa
     1801 gtcgcggtta ttgacgccga tgatgctggc gcccgcagac tcagcacgag ccagttcgtc
     1861 ctcatcgtga acctcgacga gcgcatcgag tccagtggcg ccggcgaagt ccaacaaccg
     1921 cttgagcgtc gactgatcca gcgcggcgac aatcaggagc acagcatcgg cgccggctgc
     1981 ccgtgcttcc agaatctggt actcgtcaat cacgaagtcc ttgcgtaata ccgccattgg
     2041 cgtcggccca gcgtgagcaa cctcggccgc tgcgctcagg tccgcaaggg aaccctggaa
     2101 gaacggctca tcggtcagga tcgacatggc cgccgccccg ccgatcgcat actcagctgc
     2161 gacgcctgcc gggtccatct caaccggaaa tcgtcccttc gacg
//
LOCUS       DASSGX010000549         5017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1076071, whole genome shotgun sequence.
ACCESSION   DASSGX010000549 DASSGX010000000
VERSION     DASSGX010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076071"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="VFI12_09915"
     CDS             <1..825
                     /locus_tag="VFI12_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HET7056767.1"
                     /translation="QRPNSVQEAISLLAGDPDAKVLAGGHSLIPAMKLRLAMPGTLVD
                     IGRIAELKGISVNGGATIGAMTTYDELQSSAELAKAFPVIKETVNVVGDPAVRARGTL
                     GGSLAHSDPAADFTAVMLALGASVKATGPNGEREISADDLFVDLLTTSLEPEEILTSI
                     SVPGLNGAGAAYEKHAHPASGYAVVGVAAVIKVDNGTISSARVAVTGATSKATRATAT
                     EQALVGKPANAETIAAAAAKATEGLELNGDVYASAEYRGQLIKVLAKRAITRAAGL"
     gene            1111..2808
                     /locus_tag="VFI12_09920"
     CDS             1111..2808
                     /locus_tag="VFI12_09920"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HET7056768.1"
                     /translation="MVAGQDTPTLREQVLSRRRLIQAGGGTALAAVALGDTFFARPHF
                     TAAQAISTIATDLSLEGDQFSKGDMPAGGGFSTASADRGLTVQAGLSNAQYVSPVIKT
                     DFGMTHVGPNWSADYTGGRFTVEVRLSPDGKRWTEWTPVSIETFELDPDRPGRPGIFG
                     TLVNGYGANRVQYRIRFDTRYGPVSIRRMTMTAMNVNDGPRATVLVPGEGGQVSAAPY
                     IAKPRPITRAGWGCDERLGYDSSGNEIWGKEYATWRVVVPHHTATTNNYWDAAAEVRA
                     IYYYHAVTHGWGDIGYHALVGNNKETYEGRKSPDGQILAHDLMAGHVLQCNAGSFGFS
                     FIGDFSSVYIPSGMMRAGQKLAAWVCAERGINPLGQVTFERSDRSTYRGPAIAAHRLL
                     QRPDLYPTACPGDVGYTQFPWFRQYVQDKLNAAPDDGQDPTPPSTKRYIFTGSGRSAN
                     STVSTLAWDNQTSTFWETTSGSPPKSARIYFDLGSEKYVSRVIWLFQRTGWADWCTIE
                     WSNDRTNWNFLADASNPTAKVWYERLVNRKIRYVRFVFLNPNKDAKLGGLAEVRCFP"
     gene            complement(2817..3470)
                     /locus_tag="VFI12_09925"
     CDS             complement(2817..3470)
                     /locus_tag="VFI12_09925"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HET7056769.1"
                     /translation="MQIYAHRGASRTHAENTIGAFAEAVRLGVDGIELDIHATSDGVP
                     VVIHDSSLGRTAGADFEIPDLTLDRLRSIAPTIPTFAEVLDLVGSALHFDIEVKPAGI
                     EEPVLELLGRYPQIRWSISCFDWTVLERFRELDESCDLWLLSSQMSNELVETASRLNA
                     TAAALDAPAIKEQTIKIAHDAALKVMAWTVNDVDRARQLEAWGLDMLCTDAPHLFVG"
     gene            3593..4021
                     /locus_tag="VFI12_09930"
     CDS             3593..4021
                     /locus_tag="VFI12_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET7056770.1"
                     /translation="MSSHFNEPLFVISVAARLVKMHPQTLRKYEREGLIAPSRTQGNL
                     RLYSDQDIERLRQVKYLVEDRGLNLAGVQMVLEITRRLRDLRAFTTGRGADPESGDRD
                     AYLAMTGELDRMLRSLGFIHEEAMADTRRRDNHSPEPSRG"
     gene            4080..>5017
                     /locus_tag="VFI12_09935"
     CDS             4080..>5017
                     /locus_tag="VFI12_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HET7056771.1"
                     /translation="MASNQRFTERAQEALVDAQRITQDRKLSQLEPAAILSALLSQSD
                     GIVPQIVLKAQVDLPDLIAATDRLLSTLPSLQYSSEPTVSNATRKLLDTAQHEASQFG
                     DEYVSTEHLLLGILASQTPAAQLLTERGLSRDRVLELLKSIRGTQRVTTPNPEGTYAS
                     LEKYGRDLTEMAREGKLDPVIGRDEEIRRVIQVLSRRTKNNPVLIGEPGVGKTAIAEG
                     LAQRIVRGDVPEGLQDKRIIALDMGALIAGAKFRGEFEERLKAVLNDLKKQEGQIILF
                     IDEIHTMVGAGKAEGAMDAGNMLKPALARGELHCVGA"
BASE COUNT          942 a         1591 c         1595 g          889 t
ORIGIN      
        1 cagcggccaa attctgtaca ggaggccatt tcgctcctgg ccggcgaccc tgacgccaag
       61 gtgcttgcgg gtggtcactc gctgatcccg gccatgaagc tgcggctggc gatgcccggc
      121 acgctggtcg atatcggtcg gatcgctgag ctcaagggca tctcggtcaa tggcggcgcg
      181 acgatcggcg cgatgacgac ctatgacgag ctccagagtt cggccgagct ggcgaaggcc
      241 ttcccggtca tcaaggagac ggtgaacgtc gtcggcgatc ctgctgtccg tgccagaggg
      301 accctcggcg gttccctggc ccactccgac ccggcggccg acttcacggc agtcatgttg
      361 gccctcggag cgtcagtcaa ggcgaccgga ccgaacggcg agcgcgagat cagcgcggac
      421 gacctcttcg tcgacctgct gaccacctcg ctcgagccgg aggagatcct gacttccatc
      481 tcggtaccgg gactgaacgg tgccggcgcg gcgtacgaga agcacgcgca cccggcctct
      541 ggctacgcgg tcgtcggcgt ggcggcggtg atcaaggtcg acaacggcac gatctcgtcc
      601 gcccgggtcg cggtgaccgg cgcgaccagc aaggcaaccc gcgcgacggc gacggagcag
      661 gcattggttg gcaagccggc caatgccgag acgatcgccg cagccgccgc gaaggcgacc
      721 gagggacttg agctcaacgg cgacgtctat gcgtcggccg agtaccgcgg ccagttgatc
      781 aaggtgctgg ccaagcgcgc catcacccgc gccgccggcc tctagccagc cagagtcgct
      841 gtaactggag gaggacccgc cggtttggcg ggtcctcttc gttgccccgc tcaacccgat
      901 tctcctatcc gaagttcccg cctgtcgggt gggcagaatg cgacggtctg tcatattctg
      961 ttttcaccgc acattgtccg cttgttcgga cagaggtcgt ccgcgggtta tttcgatctc
     1021 tgctcccatg aacacaacgg atattcagac gcctcgcgaa accggcaccg gaccgggtgc
     1081 gttcttcgag gcgacgggag gggaattcca atggtggctg ggcaggatac gccgacgctt
     1141 cgtgagcagg tgctgagcag gcgacggttg attcaagcag gcggcgggac cgcattggcg
     1201 gccgtcgcgc tcggtgacac gttcttcgcg cgaccacatt ttacggccgc gcaggcgatc
     1261 tccacgatcg cgaccgacct ctcgctggag ggcgaccaat tctcgaaggg cgacatgccg
     1321 gccggtggcg gattcagcac ggcgtccgca gatcgtggcc tgaccgtgca ggccggtctg
     1381 agcaacgctc aatacgtctc gccggtcatc aagaccgact ttggcatgac ccatgtcggt
     1441 ccgaactgga gcgccgatta caccggcggc cgattcaccg tcgaggtccg cctttcaccg
     1501 gacggcaagc gctggactga gtggacgccg gtcagcatcg aaaccttcga gctggatcct
     1561 gatcgtccgg ggcggcccgg catcttcgga acgctcgtga acggctacgg ggcgaaccgg
     1621 gtgcagtacc ggatccggtt cgatacgcgc tatggacctg tgtcgatccg ccgcatgacg
     1681 atgaccgcga tgaacgtcaa tgacggcccg cgcgcgacgg tgctcgtccc tggcgaggga
     1741 gggcaggttt cggcggcccc atacatcgcc aagccgcggc cgatcacgcg ggccggctgg
     1801 ggatgcgatg agcgcctcgg ctatgacagc agcggcaacg agatctgggg caaggaatac
     1861 gcgacctggc gcgtcgtcgt cccgcaccac acggccacca cgaacaatta ctgggacgct
     1921 gccgccgagg tccgcgcgat ctactactac catgcagtca cgcacggctg gggcgatatc
     1981 gggtaccacg cgctggtcgg caataacaag gagacgtacg agggccggaa gagccctgac
     2041 ggtcaaatcc tggcgcacga tctgatggcc ggccacgttc tgcaatgcaa tgctggctcg
     2101 ttcggcttct cgttcatcgg cgatttctcg tccgtctata tcccgagcgg tatgatgcgt
     2161 gccggccaaa agctggcagc ctgggtgtgc gccgagcgag ggatcaaccc gcttggccag
     2221 gtcacgttcg agcgctcgga ccgctcgacg taccggggac cggcaattgc cgcgcaccgg
     2281 ctcctgcagc ggccggacct ctatccgact gcctgcccag gcgatgtcgg gtacacgcag
     2341 ttcccgtggt tccggcagta cgtccaggac aagctgaacg ccgcgcccga cgatggccag
     2401 gatccgacgc cgccatcgac caagcggtac atcttcaccg gatccggtcg ctcggcgaat
     2461 tcaactgtgt cgacgcttgc ctgggacaac caaaccagca cgttctggga gacgacgtca
     2521 ggttcgccgc cgaagtcggc ccggatttat ttcgatctcg gctcggagaa atacgtctcg
     2581 cgcgtgatct ggctatttca gcggaccggt tgggccgact ggtgcacgat agagtggtcg
     2641 aacgaccgga ctaactggaa tttcctggcc gatgcttcga acccaaccgc caaggtctgg
     2701 tacgagcggt tggtcaaccg caagatccga tacgttcgct tcgttttcct caatccgaac
     2761 aaggatgcga agctcggcgg cctggccgag gtgcgctgct tcccgtagcg atcgcgttag
     2821 ccgacgaaga ggtgtggggc atcggtgcag agcatgtcga ggccccacgc ttcgagctga
     2881 cgtgcccggt cgacgtcatt gacagtccat gccatcactt tcaatgcggc gtcatgcgcg
     2941 attttgatcg tctgctcctt gatggccggg gcgtcgagtg cggcggcggt cgcgttcagt
     3001 cgcgacgcgg tttcgaccaa ttcattactc atctgtgaac tgagcagcca gaggtcgcag
     3061 gactcatcca gctcccggaa tcgctcgagc acggtccagt cgaagcacga gatcgaccag
     3121 cggatctgcg ggtagcgccc aagcagctcc agcaccggct cctcaatccc cgccggcttg
     3181 acctcgatat cgaagtgcag agcagacccc acgagatcca gcacctcggc aaaggtcgga
     3241 atcgtcggcg cgatcgagcg cagccggtcc agggtcaggt caggtatctc aaaatcggcg
     3301 cctgctgtcc gtccgagcga ggagtcgtgg atgacaaccg ggacgccgtc ggacgtggca
     3361 tggatgtcga gctcaatgcc gtcgacgccg aggcgtactg cttcggcgaa cgccccgatg
     3421 gtgttctcgg cgtgagtccg ggatgcgccg cgatgggcat agatctgcac ggtcctactc
     3481 ctcgtaattg atccccgccg taggtcggtt cccacagcgt tcgtctcatt ttacttgaca
     3541 tgatccgtat caacgaatat catttgcata atgaatgaga agaggggttt ccatgtccag
     3601 tcactttaac gagccgctgt tcgtaatcag cgttgctgcc cgactcgtga agatgcaccc
     3661 ccaaacgctc cgcaagtacg agcgggaagg cctgatcgcg ccatcccgta cgcagggcaa
     3721 tctgcgcctg tattccgatc aggacatcga gcggctgcgg caggtcaagt atctggtcga
     3781 agatcgtggc ctgaacctgg ccggtgtcca gatggtgctg gagattaccc gacgcttgcg
     3841 tgacttgcga gcgttcacga ccgggcgcgg tgctgacccg gaatccgggg accgggatgc
     3901 gtacctggcg atgaccggcg agctcgaccg catgctgcga tccctcggat ttatccacga
     3961 ggaagccatg gccgacaccc ggcgacgaga caatcactct cctgagccgt cccggggata
     4021 gctcaaatac atgacctgct gtaagcacgg caatgttcaa atagcggagg ttgcatcgta
     4081 tggcatcaaa tcagcgattt accgagcgcg cccaggaagc gctcgtagat gcgcaacgga
     4141 tcacgcagga ccgcaagctc tcgcagcttg agccggcggc gatcctttct gcgctccttt
     4201 cccagtcgga cggcatcgtc ccccagatcg tgctcaaggc gcaggtcgat cttccggatc
     4261 tgatcgcggc gaccgatcgc cttctgagca ctttgccgtc gctccagtac agctccgagc
     4321 cgaccgtctc caacgcgacc cgcaagctgc tcgataccgc ccagcacgag gcgagccagt
     4381 tcggcgacga atacgtcagc actgagcatc tgttgctcgg cattttggcg tcccaaacgc
     4441 cggcggctca gctcctgacc gagcgcggtt tgtcccggga tcgcgtcctc gagttgctca
     4501 agtcaattcg cggcacgcaa cgcgtgacga cgccgaatcc cgaaggaacc tacgcatcgc
     4561 tcgagaagta tgggcgcgac ctgaccgaga tggcgcggga gggcaagctt gacccggtaa
     4621 tcggccgcga cgaggagatc cgccgtgtca tccaggtgct ctcccgccgc accaagaaca
     4681 acccggtcct gatcggcgaa cccggcgtcg gcaagacggc gatcgccgag ggcctggccc
     4741 agcgcatcgt ccgcggcgac gtgcccgaag ggttgcagga caagcggatc atcgcgctcg
     4801 acatgggtgc cctgatcgcc ggcgccaaat tccgcggtga attcgaagag cgcctcaagg
     4861 ccgtactgaa cgacctcaag aaacaggaag gccagatcat cctgttcatc gacgagatac
     4921 acaccatggt gggcgccggc aaggcggaag gcgccatgga cgcgggcaat atgttgaaac
     4981 cggccttggc ccgcggcgag ttgcactgcg tgggcgc
//
LOCUS       DASSGX010000550         2090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1623667, whole genome shotgun sequence.
ACCESSION   DASSGX010000550 DASSGX010000000
VERSION     DASSGX010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1623667"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1068)
                     /locus_tag="VFI12_09940"
     CDS             complement(<1..1068)
                     /locus_tag="VFI12_09940"
                     /inference="COORDINATES: protein motif:HMM:NF021589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HET7056772.1"
                     /translation="MQWGPLSLAPEIRITDLGYDSNVFAQNDSRNPAGDFTMTATPAA
                     EAWLRLPRAQVSGRTEFDYIYYQQASSLRSWDSDSAARVDVFLARVTPYAWATLANLR
                     HRQGVEIDAPIRRIEENAVAGAAVRLSGKTSIDIRATRSRSSFEDQATYFDITLAQAL
                     NRKATGEGASVKYSVTPFTTVSLDVDRERERFDFSPERDANTLFIGPAIEMKPFAVIS
                     GRARLGLRKRTFLDAATPPYRTVAALVDLRYTLQERTGFSVEFQRDLRYSYRVVQQEY
                     VLTGFRVGVMHRLADPWDVRASVGRDRLEYGGGALGSVTVRPEVETVLRYRAEVGYRV
                     GRTRVGVHVDYRNRDAELSPRR"
     gene            complement(1525..1974)
                     /locus_tag="VFI12_09945"
     CDS             complement(1525..1974)
                     /locus_tag="VFI12_09945"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HET7056773.1"
                     /translation="MRDWQRGNEELPAAGPFDQTDAARTAGGSTTRGDRRRTARFLPK
                     RSIDVALSTVMDFTVVDLSATGVLLSCAWPLDIGHRRKLQMVIAGRPFAAWIEVRRVE
                     QFSASAGAPPGYLVGASFLSIDESSQDTLQRFIANQRRRLAARRRED"
BASE COUNT          365 a          696 c          692 g          337 t
ORIGIN      
        1 gcggcgtggg gacagctccg catccctgtt tcggtaatcg acatggacgc cgacccgcgt
       61 gcgcccgaca cggtagccca cctcggcccg ataccgcaga accgtctcca cttcgggacg
      121 gaccgtcacg gagccaagtg cgcctccacc gtattccaat cgatcgcggc cgacgctcgc
      181 ccgaacgtcc cacgggtcgg caagacgatg catgacgccg accctgaacc cggtgaggac
      241 gtattcctgc tggaccacgc ggtacgaata tctgagatcg cgctggaatt cgaccgagaa
      301 accggttcgt tcctggagcg tgtaccgcag atcgaccagc gccgcgacgg tgcgataggg
      361 gggcgtcgcc gcgtcgagaa acgttcgctt ccgaaggccc aggcgtgccc gcccgctgat
      421 gacggcaaac ggcttcatct cgatcgcggg cccgatgaac agcgtgttcg cgtcgcgctc
      481 cggcgagaag tcgaaccgtt ctcgctcccg gtccacatcg agcgagaccg tcgtgaacgg
      541 cgtcaccgag tacttgaccg aggcgccttc gccggtcgcc ttgcgattca gcgcctgcgc
      601 gagcgtgatg tcgaagtatg tcgcctggtc ttcgaacgag gaccgcgagc gggtcgcccg
      661 gatatcgatg gaggtcttgc ctgacaggcg cacggcagcg ccggcaaccg cattctcctc
      721 gatacgcctg atcggcgcat cgatttcgac gccctggcga tgccgcagat tcgcaagtgt
      781 ggcccaggcg tacggggtca cacgtgcgag aaagacatcg acccgtgcag cgctgtcgct
      841 gtcccacgat cgcaggctgc tggcctgctg gtaatagatg tagtcaaatt cggttcgccc
      901 gctgacctga gcacgcggaa gccggagcca tgcctcggcg gccggcgtgg ccgtcatcgt
      961 gaagtcgcct gccggattgc gggaatcgtt ctgcgcgaac acgttgctgt cgtacccgag
     1021 gtccgtgatc cggatctccg gagcgagcga cagcggaccc cactgcacgg gcgccctctc
     1081 cggctcgccg ctcgtttgag cgccggcatg taccgcagca caggcaagtg ccacgatcac
     1141 tccagatgag accacgccac ggcggaacca ggacacagct tcgccctctc agttacagtc
     1201 gcaggaagcg ctggaactgc cctcgctgca aggaggccgc catgggtgcg gacgcgcccg
     1261 gcgcgtcgat gccgagggaa agcgacgcgt tccgggcgcg accggggacg atcggcgggg
     1321 gttcgcgggc cggtcgtccg gcaagctcga gcgaggaccg gaggaagtct gcttcggcac
     1381 tacgtttggt ctccgtctcc tgcgacaacc gccgcgcgca tgccggatgg cacgcgagcc
     1441 ggacgcttgc cgcaatgcga aattccgggt agcagctcga aacggcctga atggcgaggg
     1501 atctggcgtg ttctctcggc gaggtcagtc ctcgcgacga cgagcggcaa gccggcgacg
     1561 ctggttcgcg atgaaccgct gcagggtgtc ttggctggac tcatcaatcg aaaggaaaga
     1621 cgcgccgacg agatacccag gaggagcacc ggccgacgcc gagaactgtt cgacccgtcg
     1681 gacttcgatc caggccgcga acggccgccc cgcgatgacc atttgcagct tccggcggtg
     1741 gccgatatcg agaggccagg cgcaggagag cagcacaccg gtggcgctca ggtcgacgac
     1801 cgtgaaatcc atgacggtgg aaagcgccac gtcgatggaa cgcttcggca ggaatctggc
     1861 cgtcctccgc cgatcccccc gagtggtcga cccgccggca gtccgcgccg cgtccgtctg
     1921 atcgaacggg ccggccgctg gtagctcctc gttgccgcgc tgccaatccc gcatagtgct
     1981 ttcattgcgt tgtgggcgag ccgcttccgc caccaggcgt ggcgtggtcc cctcggtccg
     2041 gatggaggcc tcgcgtccgg gtgatcgatc aacccgccag taccggccag
//
LOCUS       DASSGX010000551         2703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_862010, whole genome shotgun sequence.
ACCESSION   DASSGX010000551 DASSGX010000000
VERSION     DASSGX010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862010"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..834)
                     /locus_tag="VFI12_09950"
     CDS             complement(4..834)
                     /locus_tag="VFI12_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[LysW]-aminoadipate kinase"
                     /protein_id="HET7056774.1"
                     /translation="MYVIKIGGGAAIDEAAFARFAEDLAEMHAAGTPFVVVHGGNAEF
                     SKLSTALGMPPRMITNDKGRVSRYTDRETIDAMLMAYCGKVNKTIVATFQQAGINAVG
                     LSGIDGRIATGRRKPVLRGTEDGKPKVLRDDHAGSIDAVDTALISLLMANGYVPVLTP
                     PALAVDEGRPINVDGDKLALNLARELSADVLLFFSDTPGLLADKDDESTLIREIDASN
                     PSGALASAQGRMVVKVEQALKAIEHGVGQVIFADARVEHPVQRALAGEGTRIAVPVTS
                     "
     gene            complement(847..1245)
                     /locus_tag="VFI12_09955"
     CDS             complement(847..1245)
                     /locus_tag="VFI12_09955"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET7056775.1"
                     /translation="MVRSMSELIDADDLLARDLQNPEFRAEWERLAAARAVAEIVATN
                     RERKGLTQTALARAIGVRQPVIARIESGDHVPTIETLIKIASALDIEIMVGIAPSTRS
                     EPTIAGRPAGATDAEEVTAALGSRLVIAAA"
     gene            complement(1624..2670)
                     /gene="argC"
                     /locus_tag="VFI12_09960"
     CDS             complement(1624..2670)
                     /gene="argC"
                     /locus_tag="VFI12_09960"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HET7056776.1"
                     /translation="MVRVSIIGGSGYAGGEAVRLLLDHPEAELQQVTSERNAGKFVRA
                     LHPNLRKRTDLKFVSIDKLEPCDVLFVALPHGVSMGKMACLREKAPIIIDLSADFRLN
                     DPAGYPTWYDHEHTDPGMLNDFVYGIPELHRDEIRRGDLIASGGCMATTSILGLYPLY
                     RAGIVDLDRPVVIECKTGSSGSGGAPGLGSHHPERSGAMRSFKPTGHRHTAEIIQELT
                     VDGAAPEVAFSATSIEAVRGILATSHVYLKSDLSDKEVWAFYRAAYRDEPFMRVVKES
                     SGIHRYPEPKILSGSNYCDVGFERDPHSKRLVVMAATDNLMKGAAGQAVQCFNIRMGC
                     EEDLGLEFAGLHPI"
BASE COUNT          468 a          919 c          813 g          503 t
ORIGIN      
        1 gtgctaactc gtcacgggga cggctatccg cgttccttca ccggccagcg cgcgctgaac
       61 cgggtgctcg acccgggcgt cggcgaaaat cacctgcccg accccgtgct cgatcgcctt
      121 gagcgcttgc tcgaccttga cgaccatccg gccctgcgcc gaggcgagcg cgcccgaagg
      181 attggacgcg tcgatctcgc ggatcagggt cgattcgtcg tccttgtcgg ccagcagccc
      241 gggcgtgtcc gagaagaaga gcagcacatc ggccgagagc tcgcgggcca gattcagggc
      301 gagcttgtca ccgtcgacgt tgatcgggcg gccctcatcg accgccagcg ccggcggtgt
      361 caggacgggt acgtagccat tcgccatcag cagcgagatc agcgccgtgt cgaccgcgtc
      421 gatcgagccg gcatggtcgt cccggagcac cttcggcttg ccgtcctcgg tgccgcgcag
      481 gacaggcttg cgccgcccgg ttgcgatccg gccgtcaatt cccgagaggc cgaccgcgtt
      541 gatcccggcc tgctggaagg tcgcgacgat cgtcttgttg accttgccgc aataggccat
      601 cagcatcgcg tcgatcgtct cgcggtcggt gtagcggctg acgcggccct tgtcattggt
      661 gatcatgcgc ggcggcatcc ccagcgcggt actcagcttg ctgaactccg cgttcccgcc
      721 gtgaacgacg acgaacggcg ttcccgccgc gtgcatttcc gccaggtcct ccgcaaaccg
      781 ggcaaaggcg gcctcatcaa tcgccgcccc gcccccgatc ttgatgacgt acatgtgcct
      841 ctttctctat gcggccgcta tgaccaggcg gctgcccaat gcggccgtaa cctcttccgc
      901 atcagtcgct ccagccggcc ttccggcgat agtcggctca cttcgcgttg acggcgcaat
      961 gccaaccatg atctcgatat cgagcgcgct tgcaatcttg atcagcgtct cgatcgtcgg
     1021 gacatggtcc cccgattcaa ttcgagctat gaccggctgg cgtacaccga tagccctggc
     1081 caaagccgtt tgcgtcaggc ccttgcgctc ccgattcgtc gcaacgatct cagcgaccgc
     1141 ccgtgcagca gccagtcgct cccactcggc ccgaaactca gggttctgca aatcgcgggc
     1201 gaggagatcg tcagcatcga tcaattcgga catagaccga accattaacc tgcctcctca
     1261 tcactacgaa actcatcaag cctgtccaca gcgagcgtta cagcgcggtt aaatccccgc
     1321 cggtcaacat tagcctcagg acccacagct ccaatcacca tgaaatctcc aatccgacgg
     1381 tagaacgccc ggattgggct cgaacctcgt ctcgggcgca actctcgaag tgacccctgg
     1441 atcccaagca ccgtgcttga gtgcggcgcg cctagttgat ctccgagcgc cttcagcttc
     1501 tggatagcct cctggagagc tgctctttcc cgatcgggaa gtgcgtcgta ttccctcgcg
     1561 gcctccggca caaacttcac tcgccacact gattatagca tccttgctat tcgtcgaagc
     1621 gcattagatc gggtgcaggc cggcgaattc gaggccgagg tcctcttcgc accccatgcg
     1681 aatgttgaag cactggaccg cctggccggc cgcgcccttc atcaggttgt cggtcgcagc
     1741 catgacgacc agccgcttcg agtgcgggtc acgctcaaag ccgacgtcgc agtagttcga
     1801 tcccgacagg atcttcggct ccgggtagcg gtggatgccg ctcgattcct tcacgacgcg
     1861 catgaacggc tcgtccctgt aggcggcacg gtagaacgcc cagacctcct tgtccgagag
     1921 gtcgctcttc agatagacgt gcgaggtcgc gaggatgccc cggaccgcct cgatcgaggt
     1981 tgccgaaaag gccacctccg gtgcggcgcc gtccacggtc agctcctgga tgatctcggc
     2041 ggtgtggcgg tgaccggtcg gcttgaagga gcgcatcgcc ccactgcgct ccgggtggtg
     2101 cgagccgaga cccggcgcgc cgcccgagcc ggacgagccg gtcttgcact caatgacgac
     2161 ggggcgatcc agatccacga tgcccgcccg gtagagcggg tagaggccga gaatcgacgt
     2221 ggtcgccatg catccgccac tcgcgatcaa atcgccgcgc cggatctcgt cccggtgcag
     2281 ctcggggatg ccgtagacga agtcattcag catccccggg tcggtgtgct cgtggtcgta
     2341 ccaggtcggg tagccggccg ggtcgttgag gcggaagtcg gcgctgagat cgatgatgat
     2401 cggcgccttc tcgcgcaggc acgccatctt gcccatcgag acgccgtgcg gcagcgccac
     2461 gaagagaacg tcgcacggct cgagcttgtc gattgagacg aacttgaggt cggttcgctt
     2521 gcgcaagttg ggatgaagcg cgcggacgaa tttgcccgcg ttgcgctcgc tcgtgacctg
     2581 ctgcagctcc gcctccggat gatcgagcaa gaggcggacc gcctctccac ccgcgtaccc
     2641 ggaaccaccg atgatcgata cccgaaccat gattccctac tgtcccttcc aaataccccg
     2701 gtc
//
LOCUS       DASSGX010000552         2940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1515165, whole genome shotgun sequence.
ACCESSION   DASSGX010000552 DASSGX010000000
VERSION     DASSGX010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1515165"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VFI12_09965"
     CDS             complement(<1..241)
                     /locus_tag="VFI12_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056777.1"
                     /translation="MTHSAATTARAAARTTSVGLIAGTLGAIGLLDGGGGGTWTDVLA
                     SFGLSDSTLGPAFATQSAFVFPVLLLGGLLLQRVGI"
     gene            complement(238..1248)
                     /locus_tag="VFI12_09970"
     CDS             complement(238..1248)
                     /locus_tag="VFI12_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET7056778.1"
                     /translation="MTECDVYDITIIGAGPTGLFAAFYAGMRQARTQVIDSLEEPGGA
                     LTAIYPEKYIYDVPGFPKVMAKDFVEQMVLQAERDQPTMRLSEEVTNLERMDDGILRL
                     TTSKGERYSKTVIICAGVGAFEPKRIDAPGVAALEGKGVHYFAKRVDDFRDRDVVIVG
                     GGDSAVDWAVTLEPVASHVTLIHRSKFRAHEATVRELEEGRVELRYPGCEIVEAHGAD
                     EGRLHAVSYKDATGEVFRIPAHDLIVAIGFLADLGPLKTWGLDLTDRRNQIAVDKITM
                     ATNVPGVYAAGDIAWYPAKFKLIANGAAEAVTAVNHAVTFFDPSARLDAGHSTNIMAA
                     RE"
     gene            1411..2631
                     /locus_tag="VFI12_09975"
     CDS             1411..2631
                     /locus_tag="VFI12_09975"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HET7056779.1"
                     /translation="MASQVKRDYAAYVSERGQRAGDALITDPTASANYISFIYGFPDS
                     DSLPAGSVTETTHRVMTENPVWALQYGAVNGPKPIREAILAKLKRDQSISASMEEIIV
                     TAGSSQAIQLITQLLVDPGDTIIAESPSFLGFFDDVRNSGAELRGIEVDNEGLRVDLL
                     EEELNELRANGIRPSFLYILPNYQNPTGACMSVERRKRIVELAEEFDTLIIEDDAYFD
                     LRYEGDALPTIYSLDPNGRTLYLGTLSKTLAAGFRIGWVVGPEPIMRRLAALKTDGGT
                     NIFGSFIAASWLPEHFERHVTELREIYARRRDLTLDALAAHMPDGVEWSVPTGGFFIW
                     VTLPERIDTGPMLAQAREMGIEYLPGSACYFDHHGRNHMRLSFSFARDEVIEEGIRRL
                     ADLVKSEMAESISA"
     gene            2741..>2940
                     /locus_tag="VFI12_09980"
     CDS             2741..>2940
                     /locus_tag="VFI12_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056780.1"
                     /translation="MNTRLISRQELRERLPRIRLLGQSARSAIGESLRMRWRDQRRSS
                     AGLPDPVFEILVGMVSVLVPALV"
BASE COUNT          513 a          914 c          973 g          540 t
ORIGIN      
        1 gaatcccgac ccgctggagc aagagaccgc ccaggagcag aaccggaaag acgaaggccg
       61 attgggtagc gaaggccgga cccagcgtcg agtcgctcaa cccgaagctg gcgaggacat
      121 cggtccatgt cccgccacca ccgccatcga gcaggccgat cgcgccgagc gtgccggcga
      181 tgaggccgac ggatgtggtc cgggcggcgg cgcgggcggt ggtcgccgcg gagtgggtca
      241 ctctcgcgcg gccatgatgt tcgtggagtg gccggcgtcg aggcgggcgg acgggtcgaa
      301 gaaggtgacc gcgtggttca cggcagtcac ggcctcagcc gcaccgttgg cgatcagctt
      361 gaacttggcg ggataccagg caatgtcgcc ggcggcgtac acgcccggga cattggtcgc
      421 catcgtgatc ttgtcgacgg cgatctggtt gcggcggtcg gtcaggtcca ggccccaggt
      481 cttgagcggg ccgaggtcgg ccaggaagcc gatcgcgacg atcaggtcat gggccgggat
      541 ccggaagacc tcgccggtcg cgtccttgta gctgacggcg tggaggcgtc cctcgtcggc
      601 gccgtgcgct tcgactattt cgcatccggg atatcgcagc tcgacccggc cctcttccag
      661 ctcgcggacg gtcgcctcgt gcgcccggaa cttcgagcgg tggatgagcg tgacatggct
      721 ggcgaccggc tccaacgtga ccgcccagtc gactgcagag tcgccgccgc cgacaatcac
      781 cacatcgcgg tcccggaagt cgtcgacgcg cttggcgaag tagtggactc ccttgccctc
      841 gagcgcggcg acgcccggcg cgtcgatccg cttgggctcg aaggccccga cgcccgcgca
      901 aatgatgacg gtcttgctgt accgctcacc tttcgacgtg gtcaggcgga ggatgccgtc
      961 gtccatccgc tcgaggttcg tcacttcttc actgagccgc atcgtcggct gatcccgctc
     1021 ggcctggagg accatctgct cgacgaaatc cttggccatt accttcggaa atccggggac
     1081 gtcgtagatg tacttttccg ggtagatcgc ggtcagcgcg ccgcccggct cctcgagcga
     1141 gtcgatgacc tgcgtccgcg cctgccgcat gcccgcgtag aaggcggcaa agaggccggt
     1201 cggtccagcg ccgatgatgg tgatgtcgta gacatcgcac tcagtcattg cgcacatgtt
     1261 ctccagggtt cagcaacggg tttcaccaaa tgtcagggga acggcggtgt tcgtcgcgaa
     1321 agtctagcat tgccgccctg agcgattgcc gggggcagcg ggtagaattc ccggattacg
     1381 gcgtatcgaa tgaagggatg tcgggttcgt atggctagcc aggtgaagcg ggattatgca
     1441 gcgtacgtgt cggagcgtgg ccaacgagcc ggcgatgcgc tgatcacgga cccgaccgcg
     1501 tccgccaatt acatctcctt catctacggc ttcccggatt ccgactcact cccggccggt
     1561 tccgtaaccg agacgacgca tcgcgtgatg accgagaacc cggtctgggc gctgcagtac
     1621 ggggcggtca acggcccgaa gccgatccgc gaggcgatcc tggccaagct caagcgcgac
     1681 cagagcattt ccgcctcgat ggaggagatt atcgtcaccg ccggttcctc ccaggcgatc
     1741 cagttgatta cgcaattgtt ggtcgatccg ggcgacacga tcatcgccga gagtccatcc
     1801 ttcctcggct tcttcgacga tgtccggaac tccggtgcgg agctgcgcgg catcgaagtt
     1861 gacaacgagg gcctccgggt agacctgctc gaggaggagc tcaacgagct gcgggcgaac
     1921 gggatccggc cgagcttcct gtacatcctg ccgaattatc agaacccgac cggcgcctgc
     1981 atgtcggttg agcggcgaaa gcggattgtg gagctggccg aggaattcga cacgctcatc
     2041 attgaggacg acgcctattt tgacttgcgg tacgaaggcg acgctctgcc gacgatctac
     2101 tcgctggatc caaatgggcg cacgctctac cttgggacgc tctccaagac actcgcggcc
     2161 ggtttccgga tcggctgggt cgtcgggccg gagccgatca tgcggcggct ggcggcactc
     2221 aagaccgatg gtggcaccaa catcttcggc tcgttcattg ccgcgtcctg gttgccggaa
     2281 cacttcgagc gacacgttac agagctgcgc gaaatctacg cccggcggcg agacctgacg
     2341 ttggacgcgc tcgcggccca catgcccgat ggcgtggagt ggtcggtccc gaccggcggg
     2401 ttcttcatct gggtcacgtt gccggagcga atcgataccg gtccgatgct tgcccaggcg
     2461 cgggagatgg ggatcgagta cttgcccggg tcggcatgct acttcgatca ccacggacgc
     2521 aaccacatgc gcctctcctt ctcctttgcg cgggacgagg tcattgagga aggcatcagg
     2581 cggttggccg acctggtcaa atcggaaatg gctgaatcga tttccgcgta agtcgcactc
     2641 acttttgaca cgtgtcacag gtgtacgtat gctccgtaca acgtatgcct gaatcacagg
     2701 tgaatgacgg atcgctgaac ggcgtgcggt aacgcggacc atgaacaccc ggctcatatc
     2761 ccgccaggag ctgcgcgagc gactgccgcg cattcggttg ctgggccaat ccgcgcgctc
     2821 cgcgatcggc gaatccctgc gcatgcggtg gcgagaccag cgtcgatcaa gtgccgggct
     2881 gcccgatccg gtatttgaga ttctggtcgg catggtcagc gtgctcgtcc cggcgctggt
//
LOCUS       DASSGX010000553         4965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_425046, whole genome shotgun sequence.
ACCESSION   DASSGX010000553 DASSGX010000000
VERSION     DASSGX010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4965
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425046"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="VFI12_09985"
     CDS             <1..698
                     /locus_tag="VFI12_09985"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HET7056781.1"
                     /translation="AEWMVRTALSHIRILEDHDFGLIKVSLKAFEVPVLFEAYRRFAA
                     LPNDYPLHLGVTEAGTPKAGSVRSAVGLGTLLALGIGDTIRVSLTTDPVEEIFVGFEI
                     LKTLELRQKGATMIACPTCGRAEVDLFSLADKVDEFLKTVDEPIRVAVMGCEVNGPGE
                     ARDADVGLAAGRKMGVIFRKGKILKRVTEAEMLDELKAEILRAAEDKRNGVEPEPHHN
                     PNVYKPTLTLTSV"
     gene            735..1427
                     /locus_tag="VFI12_09990"
     CDS             735..1427
                     /locus_tag="VFI12_09990"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HET7056782.1"
                     /translation="MTFTVPDFMLDAATVEWLKQYDSPTVANAIETFKVRDRTEGYIG
                     GYVRALYDAGVMVGQALTVTIGQKPGKVAGRDGYWAMWEALRQMPSPAVIVMQDVTDA
                     PERYACAGEVMATLAKRLGAVGMVVDGGLRDVHEVQALGFHYYAAFPVVSHGNFEILD
                     VGVPVELDGQVVRTGDILHGDINGIVIVPPDVLDGLEDAIKVIRDRETRFMNFIKGDS
                     FSLEAAKAGTGY"
     gene            1591..2700
                     /locus_tag="VFI12_09995"
     CDS             1591..2700
                     /locus_tag="VFI12_09995"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7056783.1"
                     /translation="MRLIRIVFVMALFAMLAPAGFRGQLANAAQPLTSCEPVMRAPAT
                     PVASAAPLTVSATPTGEMKAVSVGYIPVMNFAPMFVAKELGYFAEQGLDVELRTFAGG
                     SEMIAQLATNQLNAGMGGAGPGLFNAVNTGLPVKIIAPGHSEGNPVATPLVVAKAKCE
                     SGEIRQVSDLKGKKVAINAPGATEYWLSQALAQGGLTMDDVDLQTLPFSDAVVALQSG
                     ALDAAILGEPTATLAEQQGIGVRLTTDFDVQGIQPTVVYGSEAFLKNDPEAAKGLMIG
                     YMKAVRAIMERGFSDPEILPIIAEYTNLPPELITESVAPLFTVDGSINVDGLNTLQTF
                     FRSMDELDYDTDIDPNTMIDRTALDAALQELDSSQ"
     gene            2715..3581
                     /locus_tag="VFI12_10000"
     CDS             2715..3581
                     /locus_tag="VFI12_10000"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7056784.1"
                     /translation="MAVAASHRTTRVTHPVQRTAQSAAFGPGGTLISVRDLSRTFESP
                     SGAIHALDDVSLDIRTGEFLAIVGPSGCGKTTLLRILAGLEKPTSGGLHAAGQFPSNA
                     LIFQGRSVFPWMTVEDNITYGLKLSGVARSERRDRAISLLRLIGIERFRKSYPHQLSE
                     GMRQRVAIARALAVDPDLLLMDEPFSALDEQTKLVLQDEVLDIWERTRKTIVFITHSI
                     DEALVMADRIVVMTAQPGQIKSIIDVEFPRPRDFLTVRSDPHFAERFRCVWELLKDEV
                     KMASRQPLEVPR"
     gene            3578..4354
                     /locus_tag="VFI12_10005"
     CDS             3578..4354
                     /locus_tag="VFI12_10005"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7056785.1"
                     /translation="MTRRRWEVLLSLLGPLAVLGIWEFLSRMEWINPLFWPPPTSLID
                     TLRHEISSGALFTNVRISLVRVLIGFLIAAIPGVIIGLAMGLYWPLRVLLMPVATAIY
                     AIPKLAVLPLMLIVFGVGESSKIAIIVVSIFFLIVLNTMSGVLAIDPTYRDVARNMGA
                     SRWQMFTTVAWPGALPAIFTGFRLAMGFALLVIVGTEFLDGNKRTPNGIGWYIIRSWN
                     LLKVDDLFVGLLVVAFIAWILNLLLEAIERRLLPWRPENT"
     gene            4356..>4965
                     /gene="menA"
                     /locus_tag="VFI12_10010"
     CDS             4356..>4965
                     /gene="menA"
                     /locus_tag="VFI12_10010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HET7056786.1"
                     /translation="MSTLAPTAPSTPPPLKVWLRAVRVFSFTASVTPVLIGAAFALVA
                     GEFSPLLFLLVVAASVLCHAGCNLANDYFDFRKGVDTEHSLGPSGVIQQGWLTLDQVR
                     TGMLVSFGIATVLGLIIFALSTWWLLLLAVPSLLAAILYTGGPKPLGYVALGEATVWL
                     FMGMGIVCGTFVAMARELTWEVAVGSLGISALVAAILHANNLR"
BASE COUNT          820 a         1588 c         1620 g          937 t
ORIGIN      
        1 gggccgagtg gatggtgcgg accgcgctca gccacatccg catcctcgag gatcacgatt
       61 tcgggctgat caaggtcagc ctcaaggcgt tcgaagtgcc ggtcctgttc gaggcctatc
      121 gccgcttcgc cgcgctcccg aacgactacc cgctccacct cggcgtgacc gaggccggga
      181 cgccgaaggc cggttcggtc cgctcggcgg ttgggctcgg cacgctgctc gcgctgggca
      241 tcggcgatac gatccgtgtc tcgctgacga cggatccggt cgaggagata tttgtcggct
      301 tcgaaatcct gaagaccctt gagctccgcc aaaagggcgc gacgatgatc gcctgcccga
      361 cctgcggccg cgccgaagtc gatctcttct cgctggccga taaggtcgac gaattcctga
      421 agacagtcga cgagccgatc cgggtcgccg taatggggtg cgaagtgaac gggccgggcg
      481 aggcccgcga tgccgatgtc gggctcgccg cgggccggaa gatgggcgtc atcttccgca
      541 agggcaagat cctcaagcgc gtgaccgagg ccgagatgct cgacgagctc aaggccgaga
      601 tcctgcgcgc ggcggaagac aagcggaacg gcgtcgagcc ggagccgcac cataacccca
      661 atgtctacaa gccaaccctg accctgacat cggtctaact ggtcagatta atcgcgaatg
      721 gagtgtgcct cgtgatgacc tttaccgtgc ccgatttcat gctcgatgcc gcaacggtcg
      781 agtggctgaa gcagtacgac agcccgaccg tcgccaacgc gatcgagacc ttcaaggtgc
      841 gggatcggac cgagggctac atcggcgggt acgtccgcgc gctctatgac gccggtgtga
      901 tggtcgggca ggcgctgacg gttacgatcg ggcagaagcc gggcaaggtc gccggccgcg
      961 atggctactg ggcgatgtgg gaagcgctcc ggcagatgcc ttccccggcg gtgatcgtca
     1021 tgcaggacgt gaccgatgcc ccggagcggt atgcctgcgc cggcgaggtg atggcgacgc
     1081 tcgccaagcg gctcggggcg gtcggcatgg ttgtcgatgg cggcctgcgc gacgtccatg
     1141 aagtgcaggc gctcggtttc cactactacg ccgcctttcc ggtcgtcagc cacggcaact
     1201 tcgagattct cgacgtcggc gtgccggtcg aactggacgg tcaggtggtt cgcaccggcg
     1261 acatcctcca tggcgacatc aatgggatcg tgatcgtgcc gccggatgtc ctcgacgggc
     1321 tggaggacgc gatcaaggtg atccgcgacc gggagacacg cttcatgaac ttcatcaagg
     1381 gcgactcgtt ctcgctcgaa gcggccaagg cgggaaccgg atattagtcg gccggccgtt
     1441 caccggttgg ttcccgtcaa atcggcatga ttgtgactat tgctacactc agccggtgtc
     1501 gatgagcggg ttggtagact tgccggcgcc gggcggtcgg atggaccggc acgcctcgcc
     1561 gatcgggtgc tccgtcgcac ggaggtcagc atgcgtttga ttcgaatcgt ctttgtcatg
     1621 gcgctgtttg ccatgctcgc gcccgccggg ttccgcggcc aactggctaa tgcggcccaa
     1681 ccgctcacct cgtgcgaacc ggtcatgcgc gcgcccgcga cccccgtggc cagcgcggcg
     1741 ccactcacgg tctccgcgac gccgaccggc gagatgaaag cggtctcggt cggatacatt
     1801 ccggtcatga acttcgcgcc gatgttcgtc gccaaggagc tcggttactt cgccgagcag
     1861 ggcctcgatg tcgagctgcg caccttcgcc ggcggttccg agatgatcgc ccagcttgcg
     1921 acgaatcagc tcaacgccgg catgggcggc gccggtcccg gtctcttcaa cgcggtcaac
     1981 accggcttgc cggtcaagat cattgcgccc ggccattctg aagggaaccc ggtcgccacg
     2041 ccgctggtcg tcgccaaggc gaagtgcgag tcgggcgaga ttcggcaggt atcggatctc
     2101 aagggcaaga aagtcgcgat caatgccccg ggagcgacag agtactggct gagccaggcg
     2161 ctggctcagg gcggcctgac catggacgac gtcgatctcc agaccttgcc cttctctgac
     2221 gcggtcgtcg cgctccaatc cggcgcgctc gatgctgcga tcctcggcga gccgaccgcc
     2281 accctcgccg aacagcaggg catcggcgtc cggttgacga ccgatttcga cgtccagggc
     2341 atccagccga cggtcgtcta cgggagcgaa gcgttcctga agaacgatcc ggaggcggcc
     2401 aaggggctga tgatcgggta tatgaaggcg gtgcgcgcaa tcatggagcg cgggttcagt
     2461 gatccggaga tcctgccgat catcgccgag tacacgaatc tgccgccgga gttgattaca
     2521 gagtcggtcg ccccactctt tacggtcgat ggctcgatca acgtcgacgg attgaatacg
     2581 ttgcagacgt tcttccgctc gatggacgag ctggactacg atacggacat cgaccccaac
     2641 acgatgatcg accggaccgc gctcgacgcc gcgctccagg aactcgattc gtcgcagtag
     2701 attggaatgc cgagatggca gtcgccgcgt cgcaccgcac cacgcgggtg acgcacccgg
     2761 tccagaggac cgcacaatcg gccgcattcg gccccggcgg cacgctgatc agcgtccgcg
     2821 acctgtcgcg cacctttgaa tcgccgtcgg gcgcgatcca cgcgctcgac gacgtctcgc
     2881 tcgacatccg gaccggcgag ttcctcgcga tcgtcggccc gtccggctgc gggaagacga
     2941 cgctgctccg catcctggcc gggctcgaga agccgacctc cggcggactg cacgcggcgg
     3001 ggcaattccc gtcgaacgcg cttatctttc agggccgctc cgtctttccc tggatgacgg
     3061 tcgaggacaa catcacctac ggcctcaaat tgtccggggt cgcgcggtct gagcggcgcg
     3121 accgggcgat cagcctgctc cgcctgatcg gtatcgagcg gttccggaag tcgtatccgc
     3181 accagctctc ggaaggaatg cggcagcgcg tcgcgattgc ccgggcgctg gccgttgacc
     3241 ccgatctgct gctgatggac gagccgttca gcgcgctcga tgagcaaacc aaactcgtgc
     3301 tccaggacga agtgctggac atctgggagc ggacccgcaa gacgatcgtc ttcatcaccc
     3361 actcgattga cgaggccctg gtgatggccg acaggatcgt cgtgatgacg gcccaacccg
     3421 gccaaatcaa gtcgatcatc gacgtcgaat tcccgcgacc ccgcgatttc ctgaccgtcc
     3481 gctccgatcc ccacttcgcc gagcggtttc gctgcgtctg ggagctgctc aaggacgaag
     3541 tcaagatggc gagtcgccag cccctggagg tgccccgatg acccgccggc gctgggaggt
     3601 gctgctgtcg ctgctcgggc cgctggcagt gcttgggatc tgggagttcc tgagccggat
     3661 ggagtggatt aacccgctct tctggccgcc gcccacgtca ctgatcgata cgctccgcca
     3721 cgagatcagc tcgggcgcgc tcttcaccaa cgtgcggatc agtctggtcc gtgtcctgat
     3781 cggcttcctg atcgctgcga tcccgggcgt aattattggg ctggcgatgg gcctctactg
     3841 gccgctgcgg gtcctcctga tgccggtcgc gacggccatc tacgcgattc caaagctggc
     3901 cgtgctgccg ctgatgctga tcgtcttcgg ggtcggcgag agtagcaaaa tcgcgatcat
     3961 cgtcgtctcg atcttcttcc tgatcgtgct caacaccatg agcggcgtgc tggcgatcga
     4021 cccgacctac cgggacgtgg cccgaaacat gggcgcgagc cgctggcaga tgttcaccac
     4081 ggtggcgtgg ccgggggcat tgccggcgat tttcaccggt ttccggctgg cgatgggttt
     4141 cgcgctgctg gtcatcgtcg gcaccgagtt cctcgacgga aacaagcgga cgccgaacgg
     4201 gatcggctgg tacatcattc ggtcgtggaa cctgctcaag gttgatgacc tgtttgtcgg
     4261 cttgctcgtc gtcgcgttca tcgcctggat cctgaatctg cttctcgagg cgatcgaacg
     4321 gcggttgctg ccatggcgtc cggagaacac ctgagatgag taccctcgcg ccgaccgcac
     4381 cttcgacacc gccgccgctc aaagtctggc tgcgcgcggt ccgggtcttt tcgtttaccg
     4441 cgtcggtgac gccggttctg atcggcgccg cattcgcgct ggtagccggc gagttctcac
     4501 ccctgctctt cctgctggtc gtcgctgcca gcgtgttatg tcacgccggg tgcaatctgg
     4561 cgaacgacta tttcgacttt cgcaagggcg tcgacaccga gcattcactg ggtccgtccg
     4621 gcgtgatcca gcagggctgg ttgaccctcg accaggttcg gaccgggatg ctcgtcagtt
     4681 tcggtatcgc gactgtgctc gggctgatca tcttcgcact ttcgacctgg tggctgctcc
     4741 tgttggccgt gccgagcttg ctggcggcca tcctttacac cggcgggcca aaaccgctcg
     4801 gctacgtcgc gctcggcgaa gccaccgttt ggctgttcat ggggatgggc atcgtctgcg
     4861 gcacattcgt ggcgatggcc cgtgagctga cgtgggaagt ggcggtcggg tcgctcggga
     4921 tcagcgctct ggtggccgcg attctgcacg ccaataacct ccgcg
//
LOCUS       DASSGX010000554         3386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_425094, whole genome shotgun sequence.
ACCESSION   DASSGX010000554 DASSGX010000000
VERSION     DASSGX010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425094"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="VFI12_10015"
     CDS             <1..506
                     /locus_tag="VFI12_10015"
                     /inference="COORDINATES: protein motif:HMM:NF020905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HET7056787.1"
                     /translation="QVVDFWAVSTDDPTEFMSMVHTRSTLTKLVPQAGDILYSNVRAP
                     IVRFVEDTSPGVHDTVVAPCDLPRYHLIGVEGFHDSCSNNYMTVMKQNGITEYRQAPE
                     SFNLFMNIPWTEDGELSFEPTVSKPGDYVVFEAIRPVLMVVSSCPQDILPINSGNPVE
                     FDVEISD"
     gene            complement(496..1944)
                     /locus_tag="VFI12_10020"
     CDS             complement(496..1944)
                     /locus_tag="VFI12_10020"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HET7056788.1"
                     /translation="MMGKAYAAVDLGNSSGRVMLGRVGDDRIELSEVRRIPNAPYHRA
                     DGWHWDVRRLFDDALAGLRQGAGQAELSGIAIDTWGVDYGLVDAEGNLLDDPFSYRDE
                     RTLPMLGLGEALLAADELYAQTGCQSGPINTLYQLLADQHAGRLEPAERFLLNPDLFA
                     YWLTGEIGADETIASTTQLFDPVTRGWCWSAVDAMQLPRRLFAPVRRTGTVLGAVLPN
                     LGLPTGVPVITAAGHDTAAAFVAATGGLDQLVISIGTWSLVGVELTAPLITAAGREAN
                     FTNELGVLGTIRFLRNAAGMWCVQECLREWQERGEMIDWADLIDLADDSPAFASLFDP
                     DGRHFMEPGPMADRVMRAGDEPLDGSHGPVIRSILESLSMNHRWLLETVEGVLGRRLK
                     RIRIVGGGSRNRLLCQMTADATGRPVVAGPAEATAIGNIAMQAIATGQIADLASAREL
                     IDRSFPAETYEPNPDHALWDDEYARWRVLASR"
     gene            complement(2213..2392)
                     /locus_tag="VFI12_10025"
     CDS             complement(2213..2392)
                     /locus_tag="VFI12_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056789.1"
                     /translation="MPTSRMRDRRGLSGWLFGPRSVANGRIQIYGCSPGCIILSLIVS
                     LILSILLTLLINRVL"
     gene            complement(2397..>3386)
                     /locus_tag="VFI12_10030"
     CDS             complement(2397..>3386)
                     /locus_tag="VFI12_10030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HET7056790.1"
                     /translation="ERVVTIGGGTGSYTMLSELKRFVGRLWAVVTMTDSGGSSRRLMD
                     QFGHPLPLGDLRQALVALSRSSRLWSDLFRYRFPDAPNSGIGGHSLGNLILHALEDLN
                     GGELLGALEDAQDLLDTAGRVLPVTLQQATLCACLRDGTVVRGETEIDRPGDRSVAPI
                     EQVSLDPPVSALPQVRKALTRADKILIGPGDLYTSVVACLLVDGVRDAIQSSDGEVIY
                     ICNVMTKYGETDGYRASDFVRVIHGYLGRRVDTVMVNTAAPSAELAERYASERSAMVT
                     PDLEAVRALVPQVRAGRFAITEPLMRHDAERVILGIWPELQADAAYAGPAVMD"
BASE COUNT          592 a         1171 c         1087 g          536 t
ORIGIN      
        1 atcaggtcgt cgatttctgg gcggtctcga ctgatgaccc gaccgagttc atgtcgatgg
       61 tccacacccg ttccaccctg accaagctcg tcccgcaggc cggcgacatc ctctactcca
      121 acgtccgcgc gccgatcgtc cgtttcgtcg aggacaccag cccgggcgtg catgacacgg
      181 tcgtcgcgcc ctgcgatctg ccccgctacc atctgatcgg cgtcgagggc ttccacgaca
      241 gttgctcgaa caactacatg acggtcatga agcagaacgg catcaccgag taccggcagg
      301 cgccggagtc gttcaacttg ttcatgaata tcccctggac cgaggatggc gagctcagct
      361 tcgagccgac ggtctcgaaa ccgggcgatt atgtcgtgtt cgaggcgatc cgtccggtgc
      421 tgatggtcgt ctcctcgtgc ccgcaggaca tcctgccgat caacagcggc aacccggtcg
      481 agttcgacgt cgagatcagc gactagcgag gacccgccaa cgggcgtact cgtcgtccca
      541 gagggcatga tcagggttcg gctcgtacgt ctcggccggg aatgagcggt cgatcagctc
      601 ccgggcggat gccagatcgg cgatctgccc ggtcgcgatc gcctgcatgg cgatgttgcc
      661 gatcgcggtc gcttcggccg gtccagcaac gaccgggcgg ccggtggcgt cggcggtcat
      721 ctggcagagc agccggttgc gcgacccgcc gccgacgatc cggatccgtt tgaggcggcg
      781 gcccagcacg ccttcgacgg tctcgagcag ccagcggtgg ttcatcgaga ggctctcaag
      841 gatgctgcgg atgacgggcc catggctgcc gtcgaggggc tcgtccccgg cccgcatgac
      901 ccgatcggcc atcgggcccg gctccatgaa gtggcggcca tccgggtcga acagcgaggc
      961 gaaggcgggc gagtcgtcgg ccaggtcgat caggtcggcc caatcgatca tctcgccgcg
     1021 ctcctgccac tcgcgcaggc actcctggac gcaccacatc ccggccgcat tgcgcaggaa
     1081 gcggatcgtg ccgaggacac cgagctcgtt cgtgaagttc gcctcccgcc ccgccgcagt
     1141 gatcagcggc gcggtcagct cgactccgac cagcgaccat gtcccgatcg agatcacgag
     1201 ctgatccagg ccgccggttg ccgcgacgaa cgctgccgcc gtatcgtgcc cggccgccgt
     1261 aattactgga acgccggtcg gcagtccgag attcggcagc accgcgccga ggacggttcc
     1321 cgtgcggcgc accggcgcga agaggcggcg cgggagctgc atggcatcga ccgcgctcca
     1381 gcaccaaccc cgcgtgaccg ggtcgaagag ttgcgtggtc gacgcgatcg tctcgtccgc
     1441 gccgatctca ccggtcagcc agtatgcaaa gagatccgga ttgagcaaga aacgctctgc
     1501 cggctccagt cgacccgcat gttggtccgc caggagctgg tagagggtat tgatgggccc
     1561 ggactggcaa ccggtctgcg catagagctc gtccgccgcg agcagggcct cgccgaggcc
     1621 gagcatgggc aacgtgcgct catcccggta cgagaacggg tcgtccagca gattcccctc
     1681 cgcgtcgacg aggccgtaat cgacgcccca ggtatcgatg gcgattccgc tcagctccgc
     1741 ctgcccggct ccttgccgca agccggccag cgcatcgtca aacaaccggc gtacgtccca
     1801 atgccaacca tcggcgcggt ggtacggtgc gttgggaatg cgccggacct cggacagctc
     1861 gatccggtcg tcgccgaccc gcccgagcat cacccgcccg ctcgagttcc ccagatccac
     1921 cgccgcgtac gccttgccca tcatccccgc ctgattgacc tgactctccc gatgagcggg
     1981 atggtagcgg ttccgtgcaa caaaacggag ccccgccggc atcgttcatc cccacgcccg
     2041 tttgcatgca ccacaccccg gcgtagcgcg gtccccccgt gggtcgcccg ggctccgccc
     2101 ctacgcgcac gttcccgggg accaaccgcc gtgaccggga ccggcgtcat gaatagcgtg
     2161 aggagatgca tgatgcggcc tacgggggga ccgcgatatg tcagttccct gtctagagga
     2221 ccctgttgat gagcaatgtg agcaggatcg aaaggatcag cgagacgatc agcgagagga
     2281 tgatgcagcc gggagagcag ccgtagatct ggatccggcc gttggcgaca gagcgcggcc
     2341 cgaacaacca tccgctgagc ccacgccgat cccgcatgcg cgatgtgggc atccgattaa
     2401 tccatcaccg cgggaccggc atacgcggca tccgcctgca attccggcca gatgccgagg
     2461 atcacccgct cagcatcgtg gcgcatcaac ggctcggtga tcgcgaaccg gccggcgcgc
     2521 acctgcggca ccagcgcccg cacagcctcc aggtcgggcg tgaccatcgc cgatcgctcg
     2581 gacgcgtatc gctcggccag ctcggccgat ggcgcggccg tgttgaccat gacggtgtcc
     2641 acccgccggc caagatagcc gtggatcacc cgaacgaaat cggacgcccg atagccgtcc
     2701 gtctcgccgt acttggtcat cacgttgcag atgtagatca cctcgccgtc gctcgactgg
     2761 atcgcgtccc gcaccccgtc cacgagcaga caggccacga cgctcgtgta gagatcgccc
     2821 ggtccgatca ggatcttgtc ggccctggtc aatgccttgc gcacctgcgg caatgcggag
     2881 accggaggat cgagggagac ctgttcgatc ggcgcgacgg agcgatcacc gggccggtcg
     2941 atctcggtct cgccacggac cacggtgccg tcgcgcagac aggcgcagag cgtcgcctgt
     3001 tgcagcgtca ccggcaagac gcgccccgcg gtatcgagca gatcctgggc gtcttcgagc
     3061 gcgccgagta actcaccgcc gttgagatcc tcgagtgcgt gcaggatcag attgccgagc
     3121 gaatgcccgc cgatgccgct gttcggcgcg tccgggaagc ggtagcgaaa gaggtcgctc
     3181 cagagccggc tggagcggga aagcgccacc agcgcctgcc gcagatcgcc gagcggcagt
     3241 ggatggccga actggtccat cagccggcgc gacgacccgc cgctgtcggt catggtaacg
     3301 accgcccaga gtcggcccac gaagcgtttc agctcagaca gcatcgtata tgagccggtc
     3361 ccgccgccaa tagtgaccac gcgttc
//
LOCUS       DASSGX010000555         2840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_642747, whole genome shotgun sequence.
ACCESSION   DASSGX010000555 DASSGX010000000
VERSION     DASSGX010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642747"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="VFI12_10035"
     CDS             complement(<1..492)
                     /locus_tag="VFI12_10035"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET7056791.1"
                     /translation="MPDRTIAHISDTHLGYRAYFRAGPDGRNLRSIDVEDAYRAVVDD
                     VIRHGGFDLIVHSGDVFHQSRPSWSAIRCFIEQTRRLEQLGVPIIVIAGNHDTAQLRT
                     ANTVFSVLELALPNVCFVSGYEERIFDLESLGLNVVAVPHGRLSGGPLSDIPLRPDRQ
                     NMLV"
     gene            complement(542..1309)
                     /locus_tag="VFI12_10040"
     CDS             complement(542..1309)
                     /locus_tag="VFI12_10040"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HET7056792.1"
                     /translation="MTDRNPYLLAHLAPHDLMERGIDTAILPVGATEYHGNHLPYSTD
                     TIMAEGLSLRLAKELETAVVLPPIAYGMSLHLMAWPWTLSLRPETLFAVVVDIAESLL
                     SQGITKLLVVSTHDGNPAPIENAAREINDRHGMAIALFRGWQGTAKSLLAGQYDIDED
                     HGGQSEMSVILYLAPALARQEEAVDVPNQQMDHPIRVFGSFDRVVPHGYSGTPSRGTA
                     EEGEAILDAIAAHVYPFLRQLSAENWQNGAWMSGISR"
     gene            complement(1321..2301)
                     /locus_tag="VFI12_10045"
     CDS             complement(1321..2301)
                     /locus_tag="VFI12_10045"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7056793.1"
                     /translation="MTTLITGGIGWVPSHITRAIASTGEHVVTYDLMEPDEMFRELLG
                     DLSANVTTIPGDTTDADHLRSVCEQFAVTSIIHAAAITPRRDREQGEPARIIDVNLGG
                     TVIALDVARQLPIFRRFIYISSGSAIGNVPGAAVIDESTPSHATSLYGITKHTSERVV
                     SRYRDLFGLDAVSVRLANVFGPMERVTPGYVGATELREMLRIHFDGESVRVNSLAGPW
                     LDWTYVGDIAEGVQRLWEAPSLQHDVYTNTCGQAFSIGDVLDAFGRHLPGFRYEQVER
                     DRANYLVSGSEPGAVPSNARMRDELGWTPPTSFDEGMRQYLGWIQQHGPQ"
     gene            2349..>2840
                     /locus_tag="VFI12_10050"
     CDS             2349..>2840
                     /locus_tag="VFI12_10050"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7056794.1"
                     /translation="MNGERLNVLLRRARADDAEDVSRWRNQPSSLRFQASSPRTVEQI
                     RELLGSQADVEIGPEAVGKLYWIIEVEGAPSGQVQLALDSGYRQQLMATLGYTVTDEM
                     QGRGVATEAVRRAIWIAFGPDGLDLERIEAVAAVENVASRRVLSKAGFQFEGIRRGLL
                     RIQG"
BASE COUNT          491 a          847 c          994 g          508 t
ORIGIN      
        1 gaccagcatg ttctgccgat cggggcgcag tgggatatcg gacaatggac cgccggaaag
       61 cctcccgtgc ggaacggcca ccacgttcag accgagcgat tcgagatcga agattcgctc
      121 ctcgtagccg gagacgaagc agacattcgg cagggccagc tcgaggacgg aaaacacggt
      181 atttgccgtg cggagctggg cggtgtcgtg attgccggcg atgacgatga tcggcacccc
      241 gagttgttcc aggcggcgcg tctgctcaat gaagcagcgg atcgccgacc aggaggggcg
      301 gctctggtga aagacgtcgc ccgaatggac gatcaagtcg aacccgccgt gccggatgac
      361 atcgtcgacc actgcgcgat acgcatcttc gacgtcgatc gagcgcaagt tgcggccgtc
      421 ggggcccgcc ctgaagtagg cccgatagcc gaggtgcgta tcggagatgt gggcgatggt
      481 gcggtcaggc atgcgcgatt gaggactcca cgcccaatat tagcacatac gtacgccgtc
      541 tttagcggga aatgccgctc atccaggcgc cgttttgcca gttctcggcc gagagctgtc
      601 ggaggaacgg gtagacgtgc gcggcaattg cgtcgaggat cgcttcgccc tcttcggctg
      661 tcccgcgcga cggcgtgccg ctgtatccgt gcggcacgac ccgatcgaac gagccgaaga
      721 cgcgaatcgg gtggtccatc tgctggttcg gcacgtcgac cgcctcctcc tgtcgcgcca
      781 gggccggggc gaggtagaga atgaccgaca tttccgattg cccgccgtgg tcctcatcga
      841 tgtcgtactg gcccgcgaga agcgatttcg ccgtgccctg ccagccgcga aacaacgcga
      901 tcgccatgcc gtggcggtca ttgatctcgc gggcggcgtt ctcgatcggg gctgggttgc
      961 cgtcatgggt ggagacgacg agcagcttcg tgatgccctg ggagaggagc gactctgcta
     1021 tgtcgaccac caccgcgaaa agcgtctccg gccgcagcga gagtgtccac ggccacgcca
     1081 tgaggtgcag cgacatgccg taggcgatcg gtggcaggac gacggccgtc tccagctcct
     1141 tcgccaaccg cagagacagt ccctctgcca tgatcgtgtc ggtgctgtag ggcaggtggt
     1201 tgccgtggta ctcggtcgcg ccgaccggca agatggcggt gtcgatcccg cgctccatga
     1261 ggtcatgcgg cgcgagatgg gcgagcaggt agggattgcg gtcggtcacg gatgctcctt
     1321 ctactgtggg ccgtgctgct ggatccagcc gaggtattga cgcatgccct cgtcgaatga
     1381 agtgggggga gtccagccga gctcgtcccg catccgcgca ttcgacggca ccgctcccgg
     1441 ctcgctgccg gagacgaggt agttcgcccg atccctctcg acctgctcgt atcggaaacc
     1501 gggcagatgc cgaccgaagg catcgagtac gtcgccgatc gagaatgcct ggccacaggt
     1561 gttggtgtag acatcgtgct ggaggctggg cgcctcccaa agccgctgga cgccctcggc
     1621 gatgtcgccg acgtaggtcc agtcgagcca gggaccggcg agcgagttga cccgaaccga
     1681 ctcgccatcg aagtggatcc gcagcatctc gcgcagctcc gtcgcgccca cgtacccggg
     1741 tgtgacccgc tccatcggcc cgaagacatt cgccagccgg accgagacgg cgtcgagacc
     1801 gaagagatcg cgataccggc tgacgacccg ctccgaggtg tgcttggtga tgccgtagag
     1861 gctggtcgcg tgggacggag tcgactcgtc aatgactgcc gcgccgggca cgttgccgat
     1921 tgccgatccc gaggagatgt agatgaagcg ccggaagatc ggcaactggc gggcgacgtc
     1981 gagcgcgatg accgtcccgc cgagattgac gtcgatgatc cgggccggct cgccctgctc
     2041 ccggtcccgc cgcggcgtga tcgccgccgc atggatgatc gaggtgaccg caaattgttc
     2101 gcagaccgag cgaaggtggt ccgcgtccgt ggtgtcaccc gggatggtgg ttacgttcgc
     2161 gctcaggtca ccgagcagtt cccggaacat ctcatccggc tccatcagat cgtacgtgac
     2221 gacgtgctcg cctgtggaag cgatcgcccg ggtgatgtgg ctgggcaccc agccgatgcc
     2281 gccggtgatg agggtggtca cgattcctcc cgcttctgat agagtccgct tcgggaggca
     2341 ttattgcggt gaatggggaa cggctcaacg tgctattgcg ccgggcgcga gccgacgacg
     2401 ccgaggacgt ctcgcggtgg cgtaatcagc cgtcatcgct ccggttccag gcaagctcgc
     2461 cgcggacggt cgagcagatt cgggagttgc tcggtagcca ggcagacgtg gagattggtc
     2521 ccgaggccgt gggcaagctc tactggatca tcgaggtcga gggggctccg tccggacaag
     2581 tccagcttgc tttggattcg ggctatcgtc agcagttgat ggcaaccctc gggtataccg
     2641 tgaccgacga gatgcagggg cgcggcgtcg cgaccgaggc ggtgcggcgg gcgatttgga
     2701 tcgcatttgg tccggatgga ctcgatctgg agcgaatcga ggcggtcgct gcggtcgaga
     2761 atgtggcgtc gcggcgggta ctttcgaagg ccgggtttca gtttgaaggg atccggcgcg
     2821 ggttattgcg gattcagggg
//
LOCUS       DASSGX010000556         2975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1294296, whole genome shotgun sequence.
ACCESSION   DASSGX010000556 DASSGX010000000
VERSION     DASSGX010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294296"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="VFI12_10055"
     CDS             complement(<1..388)
                     /locus_tag="VFI12_10055"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HET7056795.1"
                     /translation="MKINTAKAKMLAGGTAFGFELGMGSALIAEALSRTGIDYLQIDT
                     QHGDWGPDSTLAALVAMEGGTATPVARVARNDYTLIGRLLDEGMMGIVVPMVHTAEDA
                     RAAADACRFPPLGTRSCGWGRVGRNDP"
     gene            complement(504..1232)
                     /locus_tag="VFI12_10060"
     CDS             complement(504..1232)
                     /locus_tag="VFI12_10060"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET7056796.1"
                     /translation="MGNAVDLNTMLSLPANRGLGVDIAERLRSAILNGYFGPGERLPE
                     EQLARTMGVSRGPVREALATLEREGLIVIRRNRGAFVAQLSRKDLDEVYSLRVAIERL
                     AVERCVANADDADLAELQGVVDRIAELMERGITEQDAAELDLEFHDIIYRSGRHRRLY
                     DTWATLRPQIHVLLLNRNVAHEDFRDLVVPSHQAIIDAIRSRDTELSVELTMAHLKES
                     YDKVRDARQYAPGHTEDKSKGDHA"
     gene            complement(1424..1798)
                     /locus_tag="VFI12_10065"
     CDS             complement(1424..1798)
                     /locus_tag="VFI12_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056797.1"
                     /translation="MRPSELKRVIATIQSAYFMMTGIWPILHVRSFEWISGPKVDRWL
                     VKTVGALITVIGATIGLGASRRRVPPEIEFLATGSAVSLDLIDDIYVAKRRIRWVYLL
                     DAAAEFALAAAWARTRMRQTPG"
     gene            1920..2084
                     /gene="rpmG"
                     /locus_tag="VFI12_10070"
     CDS             1920..2084
                     /gene="rpmG"
                     /locus_tag="VFI12_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002516218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET7056798.1"
                     /translation="MARKKGDRIVIKLKSTESEYIYYSEKNKRNDTARLELKKYDPLL
                     RRHVVFREAR"
     gene            complement(2154..2735)
                     /locus_tag="VFI12_10075"
     CDS             complement(2154..2735)
                     /locus_tag="VFI12_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056799.1"
                     /translation="MEKALTGLFGSGPDADEKTQRAQDFVQRYSQGKPDEGYSTDEAV
                     GQLNNLLGHANSDQVERATKQALDNLPENQRTEFGQFVNQLHQRQTGQASSGTPSVTD
                     ISRMFGQAGGSATSVGDLFGGLFGGGGGGAITSMLGGLLGGGGQTHATQQSGQEQDGG
                     MGSLSDFMQSGVGKVVLGGIAAYLAKELMNQHR"
BASE COUNT          489 a          907 c          996 g          583 t
ORIGIN      
        1 cggggtcgtt ccggccgacc cggccccagc cacaggagcg cgtcccgagc ggcgggaagc
       61 ggcaggcgtc ggccgcggcc ctggcgtcct cagcggtgtg gaccattggc acgacgatac
      121 ccatcatgcc ctcgtcgagg agccgtccga tcagcgtgta gtcgttccgg gcgacccgtg
      181 ccaccggcgt ggccgtgcca ccctccatcg cgaccagcgc cgcgagcgtg ctgtccggac
      241 cccagtcgcc gtgctgggta tcgatctgga ggtagtcgat cccggtgcgg gagagggctt
      301 cggcgatgag cgcagagccc atgcccaatt caaacccgaa cgccgtgcca ccggcgagca
      361 ttttggcctt cgcggtattg atcttcattg attcctcgtg cggcatcacc gcgtcagcca
      421 ggtaaacggt cgctcgattt tgcgagcgac attgtgcagg aatcgggccg ccggcgcgcc
      481 atcgaccacg cgatgatcga aggtcaggcg tggtcgccct ttgacttgtc ctcggtgtga
      541 ccgggcgcat attgccgcgc atcccgcacc ttgtcgtagg actccttgag gtgtgccatg
      601 gtcagctcga cgctgagctc ggtatcccgg ctgcggattg catcgatgat ggcctgatgt
      661 gacggtacga ccaggtcgcg gaagtcctcg tgggcgacgt tgcggttgag gaggaggaca
      721 tggatctgcg ggcggagcgt ggcccaggtg tcgtagaggc gacggtgccg tccggagcga
      781 tagatgatgt cgtggaactc gaggtcgagc tcggcggcgt cctgttcggt gatgccgcgt
      841 tccatcagct cggcgatgcg atcgacgacg ccctggagct cggcgagatc cgcgtcgtcc
      901 gcattcgcca cgcagcgttc gacggccagc cgttcaatgg cgacgcggag ggagtagacc
      961 tcgtccaaat ctttgcggga gagctgggcg acgaacgcgc cgcggttgcg acggataacg
     1021 atcagcccct cgcgctcgag cgtggccagg gcctcgcgaa ccgggccgcg gctgacgccc
     1081 atggtccggg caagttgctc ttcgggcagt cgctcgccgg gaccgaagta gccgttgagg
     1141 atcgcgctgc ggaggcgctc ggcgatgtcg acgccgaggc cgcggttggc cggaagggag
     1201 agcatggtat tcagatcgac ggcattgccc atgggtcggg gttcctgacg tagaccgggg
     1261 cagactgaca atccaggatc cggtcgtttc tggttgattg tcgattgtcg acaatctcgc
     1321 agatgccgtt cgggctgtca agggggcggt tgcggcgagc aatcggggcg cggaggcgag
     1381 aagattgtcg ggcagatggc gtctctccgt tccggggagt cgcctagccg ggggtttgtc
     1441 tcatccgggt acgcgcccaa gcggcggcga gggcgaattc ggcggcagcg tcgaggagat
     1501 agacccaacg gatgcggcgc ttggcgacgt agatgtcgtc gatcaggtcg agggataccg
     1561 cgcttcccgt ggcgaggaac tcgatctccg gtggtacccg gcgcctcgaa gcgccgagtc
     1621 cgatcgtcgc accaatgacc gtgatcaatg cgccgacggt cttgaccagc cagcggtcga
     1681 ctttcggtcc gctgatccat tcgaaactgc ggacgtgcag aatcggccag atcccggtca
     1741 tcatgaagta ggcggactgg atcgtggcga tcacgcgttt gagctcagat gggcgcattt
     1801 tggtccctct ccgacatgct cgctccgctg gtcaggaacc tgtgtttcgg ctatagtgga
     1861 ccggttgacg ggggcccgga cgcccttttg ttttgtgtgc gcaaggagcg cttgtcgaca
     1921 tggcacgaaa aaagggcgat cgtatcgtca tcaagctcaa gagcaccgag agcgaataca
     1981 tctactactc cgagaagaac aagcgcaacg ataccgcgcg gctcgagctg aagaagtacg
     2041 atccgctgct ccgccggcac gtggtcttcc gcgaagcccg gtaatcactc tcccgcgata
     2101 ttgaacgagg gccggtcgca tgaccggccc tcgttgtgtt ttccacttga tcgctaccgg
     2161 tgttggttca tgagctcctt ggcaaggtag gccgcgattc cgcccagcac gaccttgccg
     2221 acgccgcttt gcatgaagtc gctgagactg cccatgccgc catcctgttc ctgcccggac
     2281 tgctgcgtcg cgtgcgtctg acctcctccc ccgagtaacc cgccgagcat gctggtgatc
     2341 gcaccaccac caccgccgcc gaagagtccg ccgaacaggt cgccaaccga ggtggcggag
     2401 ccgccggctt gcccgaacat gcgcgagatg tcagtgacgg acggtgtgcc gctgctcgcc
     2461 tggcccgtct gccgctggtg cagctggttg acgaactgac cgaactcggt ccgttggttc
     2521 tccggcaagt tgtccagtgc ttgcttcgtc gcccgttcga cctggtcgga attggcgtgc
     2581 ccaagcaggt tgttgagctg cccaacagcc tcgtccgtcg aatatccttc atcgggcttg
     2641 ccctgcgagt agcgctgcac gaagtcctgc gcgcgctggg ttttttcatc cgcgtcgggt
     2701 ccgctgccga acaaaccggt taatgctttc tccatcgaag gtctccttcc tttccggccg
     2761 attatccgta gtagcgcgga ggaagctgat tgccacgcta cgctaggctg cgcgccatgc
     2821 ggcaggtggc gttcgtaccg gagggttctc gttgatgcgc aaattgctga tcattgcggt
     2881 cattctttcg atgttcggca gcatcggcgt catgccggtg cgggcggatg aacagcagga
     2941 catcaccggc gcgcgcgctc gggccgagga gatct
//
LOCUS       DASSGX010000557         3254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_971415, whole genome shotgun sequence.
ACCESSION   DASSGX010000557 DASSGX010000000
VERSION     DASSGX010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_971415"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..520
                     /locus_tag="VFI12_10080"
     CDS             65..520
                     /locus_tag="VFI12_10080"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET7056800.1"
                     /translation="MTTSTVSIRPFASADQAAVRTLVLAGLQDHWGTLDPTLNPDLND
                     IADWYASLDGYTVVAEIDDQIVGTGTMHRADDQTGVLVRMSVSRDHRGRGIGKALVSA
                     LADRARLRGYSRLVCETTDTWQDAIALYRATGFTIIDQRNGDYHFEMQL"
     gene            complement(639..1910)
                     /locus_tag="VFI12_10085"
     CDS             complement(639..1910)
                     /locus_tag="VFI12_10085"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7056801.1"
                     /translation="MNKVQAVIGRRVFYGWVVVGVAVLLSLVMWGIRAAPSVLIKPLE
                     ADFGWSRAEISSAIAIGLVMTAFSAIVGGVLIDRYSMRRVMFGIVVLGGFSVVLASTM
                     TALWQMTVLWGLLVGIATGISGVIGAPLAARWFVEKRGLVQGILGAGTSAGQLIFLPV
                     MTWMVVQFGWREATLVLGLLAFAFVPVIVLFMRDQPADVGLRAFGATEELPRVARPPF
                     RFVLGRVRRVPDFWLLAGSFFVCGATSNGLIGQHFQAHAIDHGLTEYNASTAFAIMGG
                     LNFVGVLASGYFTDKYDPRRLLMIYYTFRGISLFFLPMLTDFGLSGLTFFSILYGLDW
                     IATVPPTMTLCAQLFGRRNVGTVYGFVFGAHQLGAAMAAFGAGYIHDAVGDYTWAFLS
                     GGVMCLVGGMLARRIDRAASWESTLAMSPAD"
     gene            complement(2176..2841)
                     /locus_tag="VFI12_10090"
     CDS             complement(2176..2841)
                     /locus_tag="VFI12_10090"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HET7056802.1"
                     /translation="MLLANLREQVIEAGLEAHRRGIVYGTAGNFSIRDPETGLIAISP
                     SGMPYGVMTPADVCIVSVDGAIEDACRRPSSETPMHTMIMQQRPDVRAIVHTHSHYCT
                     VVSTFKSCLPPILTETALVLGSRIPVTRYGETGTPDIGQSVVDVMDETTRAVIMKNHG
                     LICFGETFDQALTYAMIAEEAAHVYIDALAANGGREPDYVPEELIAKMTAHFLATYGQ
                     SGY"
     gene            2954..>3254
                     /locus_tag="VFI12_10095"
     CDS             2954..>3254
                     /locus_tag="VFI12_10095"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET7056803.1"
                     /translation="MDRSCRFEGKSVIVTGAGMGIGRGTAVAFAREGGCVIVADISGD
                     AADDTAREITSQGGKAISVTCDVSKSADVQRMVAAGVKAFGGIDVVFNNAGINLFG"
BASE COUNT          595 a         1145 c         1004 g          510 t
ORIGIN      
        1 acgtgctcga aaccgtcgtc cgcggcgtcc cgatctaccg ggccatcgac ccggacgcga
       61 ttcgatgacg acctcgaccg tctcgatccg cccattcgcg tcggctgatc aggccgccgt
      121 ccgcaccctg gttctcgccg gcttgcagga tcactggggc acgctcgacc cgacgctcaa
      181 ccccgacctg aatgacatcg ccgactggta tgcatcgctc gacggctaca ccgtcgtcgc
      241 cgaaatcgac gaccaaatcg tcggcaccgg cacgatgcac cgcgccgatg accagaccgg
      301 cgtgctcgtc cgcatgtccg tctcgcggga tcaccggggc aggggaatcg gcaaagccct
      361 cgtctccgcg ctggccgatc gggcccggtt acgcggctat tcccgcctgg tctgcgagac
      421 caccgacacc tggcaggacg ccatcgccct ctatcgcgcc accggcttca ccatcatcga
      481 ccagcgcaac ggcgactacc acttcgaaat gcagctctga ccccgcatac ccgcggacgt
      541 ttgcgatcgc gcgcacgata caccgccgcg ttcagcctcg gagaggcttc ctgatctgag
      601 cccggggttt caacaccggg ccaaccccgg aacccggatc agtccgccgg gctcatcgcg
      661 agcgtcgatt cccagcttgc cgctctgtcg atccgcctcg ccagcatccc accgaccagg
      721 cacatgaccc cgccactcag gaaggcccac gtgtaatcgc caacggcgtc gtggatgtac
      781 cccgccccga atgcggccat cgcggccccg agctgatgcg ccccgaagac gaacccgtag
      841 accgtgccga cgttccgccg cccgaaaagc tgcgcgcaga gtgtcatcgt cggcgggacc
      901 gtcgcgatcc agtccagtcc gtacaggatc gagaagaagg tcagcccgga gagaccaaaa
      961 tcggtcagca tcggcaggaa gaagagcgag atcccgcgga aggtgtagta gatcatcagc
     1021 agccggcgcg ggtcgtactt atccgtgaag taaccggatg ccagtacgcc gacgaagttc
     1081 agcccgccca tgatcgcgaa cgcggtcgag gcgttgtact cggtcaggcc gtggtcgatt
     1141 gcgtgggcct ggaagtgttg gccgatcaac ccgttcgagg tcgccccaca gacgaagaag
     1201 ctcccggcca gcagccagaa atcgggcacc cgccgcaccc ggcccaggac gaaccggaac
     1261 ggtggccggg ccactcgcgg cagttcttcg gtcgcgccga atgcccgaag cccgacgtcc
     1321 gccggttggt cccgcatgaa gaggacgatc accggcacga aggcgaacgc cagcagccca
     1381 aggaccaagg tcgcctcccg ccacccgaac tgaaccacca tccaggtcat caccggcagg
     1441 aagatcagtt gcccggctga ggtgcccgca ccgagaatgc cctgcaccaa gccgcgcttc
     1501 tcgacgaacc accgcgcggc cagcggcgcg ccgatcacgc cgctgatccc ggtcgcgatc
     1561 ccgaccagca ggccccagag cacggtcatc tgccagagcg ccgtcatggt cgaggcgagc
     1621 acgacgctga acccgccgag gaccacgatc ccgaacatca cccgccgcat gctgtaccgg
     1681 tcgatcagga cgccaccgac gatcgccgag aaggcggtca tgaccaaccc gatcgcgatc
     1741 gcggatgaga tctcggcccg gctccagccg aaatcggcct cgagcggctt gatcagcacg
     1801 ctcggcgcgg cgcggatgcc ccacatcacc agcgaaagca acaccgccac gccgaccacg
     1861 acccagccgt agaagacccg ccgcccgata actgcttgca ccttgttcac gggcgaatgg
     1921 gcctgtctat ttcagtgagt aaaggaatcg ttttcccggc gggattttaa cgtaccagac
     1981 gcagagcccc gtcccccgta gccggcatcc ttcccggaag gcaccggcgt agcgcggtcc
     2041 cccgtgggcc gcccgggccc gctggccaac ctgatcgttg gagggcacca actcccgcgc
     2101 cggttcggtg catacaaccg cgcgtgcgga cgaacggggc ggtccacagg gggaccgcgc
     2161 tacgtttcct gtgggtcaat atccgctctg cccatacgtc gcgaggaagt gcgccgtcat
     2221 cttcgcgatc agctcctccg gcacgtaatc cggctcgcgg cccccgttcg cggccagcgc
     2281 gtcaatgtag acgtgcgccg cctcttccgc gatcatcgcg taggtcagcg cctggtcgaa
     2341 tgtctcaccg aagcagatca gcccgtgatt cttcatgatc accgcgcgcg tggtctcatc
     2401 catgacatcg accacacttt ggccgatatc gggagttccg gtttcgccgt aacgcgttac
     2461 gggaattcgg gaaccaagca ccagcgcggt ctcggtcagg atcggcggca ggcacgactt
     2521 gaacgtgctg acgaccgtgc agtagtgcga gtgggtgtgg acgatcgccc gcacgtccgg
     2581 ccgttgctgc atgatcatcg tgtgcatcgg cgtctcagag ctcggccgcc ggcacgcatc
     2641 ctcgatcgcg ccgtccacgg agacgatgca aacatcggcc ggcgtcataa ctccgtacgg
     2701 catcccgctg ggcgagattg cgatcaggcc ggtctcggga tcgcggatgc tgaagttgcc
     2761 ggccgtgccg tagacgatgc cacgccggtg cgcctccagc cccgcttcaa tcacctgttc
     2821 gcgtagattt gccagaagca ccgattctct ccccgttgcg gtcgcggttc gaccccattg
     2881 tgccggtcgc gttcgtggcg ataatacggc gcacgggctg ccggcgaatg gggcggccgg
     2941 aaggagcgct gagatggatc ggtcgtgtcg gttcgagggg aagtccgtca tcgtgacggg
     3001 cgccggcatg gggatcggcc ggggcacggc ggtcgccttc gcgcgcgagg gtggctgcgt
     3061 gatcgtggcc gacatctccg gcgacgcggc cgatgacacg gcccgcgaga tcaccagcca
     3121 gggcggcaaa gcgatttcgg tcacctgtga cgtctcgaaa tccgccgacg tgcagcggat
     3181 ggtcgcggcc ggcgtcaagg ccttcggcgg cattgacgtc gtcttcaaca atgccgggat
     3241 caacctcttc ggcc
//
LOCUS       DASSGX010000558         6929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1188182, whole genome shotgun sequence.
ACCESSION   DASSGX010000558 DASSGX010000000
VERSION     DASSGX010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6929
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188182"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1249
                     /locus_tag="VFI12_10100"
     CDS             <1..1249
                     /locus_tag="VFI12_10100"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HET7056804.1"
                     /translation="ISVDPSADYEDTLAAVHETVDGYPGLYRDVQTYLKERIREVLAG
                     SSDAIVIRIYGPDLETLRAKAAEVEGILGGIDGVVEEHTELQVDIPQINIQVDLEKAA
                     TWGIKPGDVRREVGAILASFETNDLYSNGKIYDIRIVGTESTRHSLTSIENLLIDSPY
                     GGQVRLADIASVKIEKTPNEVKHEHLVRKIDVEANVRGRDLGSVVDDLNKQLASVEWP
                     LEYRMEMLGEYAERQAASQRLMYYAIAAAIGIFLLLQVSFRSFRLGLLSFLTLPSALV
                     GGVLAAYLGDGIISLGALVGFLTVLGIAARNGIMMITHFQHLEREEGIPFGPELVLRG
                     AQERLSPILMTALATGLALVPLVWAGSIPGHEIEHPMAVVILGGLVTSTLLNLFLVPS
                     LYLRFGKSDRGTETPSGPELVAA"
     gene            complement(1326..2093)
                     /locus_tag="VFI12_10105"
     CDS             complement(1326..2093)
                     /locus_tag="VFI12_10105"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HET7056805.1"
                     /translation="MADFKQKLRSGQAVSGPIVSEIRTVGLIKALANAGHDFVWLDME
                     HAMFGWETIQTLVQYARAVGITPLVRATDLSYPLVARALDSGAIGVIVPRVETVEQVE
                     AAVQYAYYPPIGRRGAGGEGRYGFERRTPADGVTEVNATTVVVVQIETTQAVERIDEL
                     AAVDGVDVICVGPQDLSISLGMPGQYDNPTFVETVTHVMERVTAAGKVCGMVERDARR
                     FQRWYDAGGRFFATNTDVNLIFTGAKADVDVVRGFGS"
     gene            complement(2094..2996)
                     /locus_tag="VFI12_10110"
     CDS             complement(2094..2996)
                     /locus_tag="VFI12_10110"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: protein motif:HMM:TIGR01505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0019580 - galactarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HET7056806.1"
                     /translation="MAEGTGQTIGFIGTGIMGKPMVRNLVKAGYRLVIHNRSQGAVDE
                     LSAESDAVTAAGSPAEVARAADTVILMLPDSPDVRAVVFGDDGVLSAVRPGFLLIDMS
                     TIAPSTAIEVSTAVGTAGGSAIDAPVSGGDKGAIAGTLSIMVGGSEEDLKRAMPLFEA
                     MGKTIVHIGGPGAGQIAKACNQVMVAINYAAVSEALVLAERSGVDPEKVAQVLGGGLA
                     NSRVLELRGQTMIKGEFQPGFRVDLHRKDLAIALATGRERESALLVTALVSQLFDALS
                     ATGGGQLDHSALITMVQRLSGSGE"
     gene            complement(2996..3775)
                     /locus_tag="VFI12_10115"
     CDS             complement(2996..3775)
                     /locus_tag="VFI12_10115"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HET7056807.1"
                     /translation="MVQFCANVSMLFAEVPFLERFGRARAAGFDAVEFQFPYEFDAKE
                     IKTELDRHNLELVLFNFPAGDFAAGDRGMANDPARIDDFKWTIETGLAYARTLKPAKM
                     NLMAGKRHGDVPDDRQRATLIANLRQVADQTAVEGILLVTEPLNPHDAPGFFLPTPSA
                     GFEIVAEANHPNAKVEYDIYHAARTEGDIVNAIRENIDAIGHIQLADAPGRNEPGTGE
                     LDWRPIFEVIDAVGYSGRIGLEYKPSTERTEDSLGWMKEYA"
     gene            3869..5782
                     /locus_tag="VFI12_10120"
     CDS             3869..5782
                     /locus_tag="VFI12_10120"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HET7056808.1"
                     /translation="MTDSHILTAEELADRRYPSAIAISNDGRFVALVIEPMGRREEHS
                     VSEIWLSRDGQPARQFSSGLAGDASPVFSPDSAKLAFLSDRHEPKTQRIYLLPLDGGE
                     ATRVSTIEGTLASLAWSPDGAHLSVLVTDPETEIEKKQKEERNDPILENQNLKYARLF
                     VIDVDSGASRQLTFGNRNVLDYAWSRDSQTLAIATSHRTDVEGEFQDVDVSLVAVTGG
                     RLRTVATLRGVASPLIFVDHPDGEQLAMRHNGFFDDPVDSVWLMSTATGEQRELLTDD
                     HGNVDWIGAVPGKPGQMAMRMVDGVHANAWLVDVTTGERTKTLPANWQDRGSLHAGPV
                     YSADGKKLAFVWSDSVTPEEAFVATVGKEPVKLTSFGESFTGRLNRSEVVEWQSDEWT
                     IQGHLVYPHGYEEGTRYPLLIEAHGGPSWQWEDHCFLDWHDWAQFMASRGFAVLAPNP
                     RGSTGRGAAFQKALQNDVGGGEVRDLITGAQAMVDLGIADPDRLGIGGWSWGGYLTAT
                     TITRTNIFKAAMMGAGLSNLFSDHGTDDIPSANLLYFPGEPYHHADRYWAGSALKCVA
                     NCTTPTLIIHGDADERVHPLQGKEMYTALSRLGIDTEFVRYPRQGHTIKERNYQIDLL
                     NRLAAWFEKYLMT"
     gene            complement(5779..>6929)
                     /locus_tag="VFI12_10125"
     CDS             complement(5779..>6929)
                     /locus_tag="VFI12_10125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HET7056809.1"
                     /translation="LGIARSLIAIPSVSGDEAVIMQWVHDWCRARELAVTTIARDELR
                     PNLIISIGDPASGSTLAMNGHLDTVPVSDADKWTTDPFDPVISEDGMRLSGRGASDMK
                     ASVGVMLAMMELFRDRPMCGCLQAHIVSDEELSGHYGTVAVMDAIADGTLPKPTYCLI
                     GEKSDLKVRNAERGILGMRVTVHGRASHTAAARTTGVNAIALAAKAILALETDIDRYH
                     PSVGKPVISINQISGGVAHNVVPGECVFTIDRRLIPGETRESVVNEITETLANVAAAE
                     PGEFRYSLVVDPDGDFIPANLTDADSPLVQSVWDATREVRGKEPEFFVQWAGATDGRY
                     YRLAGIDTVGMGPGGENAHGANEAVYIDDLVAQAKIYALTIERLLGAG"
BASE COUNT         1199 a         2358 c         2141 g         1231 t
ORIGIN      
        1 gatcagcgtc gatcccagtg ccgactacga ggacacgctg gcggcggtcc atgaaacggt
       61 cgacgggtat ccagggctct accgggatgt ccagacctac ctcaaggagc gtattcgcga
      121 ggtcctggcc ggctcgagcg atgcgatcgt gatccggata tacggtccgg acctcgaaac
      181 gctgcgggcc aaggctgctg aggtcgaggg aatcctgggc ggaatcgacg gggtggtcga
      241 ggagcacacc gagcttcagg tcgacattcc gcagatcaat atccaggtcg atctggagaa
      301 ggccgcaacc tggggcatca agccggggga cgtccgtcgc gaggtgggcg cgatcctggc
      361 cagcttcgag actaacgacc tctactcgaa cggcaagatc tatgacatcc ggatcgtcgg
      421 gaccgaatcc acccggcaca gcttgaccag catcgaaaat ctgctgatcg actcgccata
      481 cggcggccag gttcgcctcg ccgacatcgc gtcggtcaag atcgaaaaga cgccgaacga
      541 ggtcaagcac gagcatcttg tccggaagat cgatgtcgag gcgaacgtgc gcgggcgcga
      601 cctgggctcc gtcgtcgacg acctgaacaa gcaactggcg tcggtcgaat ggccgctcga
      661 gtaccgcatg gagatgctcg gtgaatacgc cgagcgccag gcggcctcac agcggttgat
      721 gtactacgcg atcgccgcgg cgatcggtat cttcttgctc ttgcaggtct ccttccggtc
      781 gttccggctc ggactgctct cgttcctgac actgccatcg gcgctcgtag gaggtgtatt
      841 ggccgcctac ctcggcgacg gcatcatctc cctgggggcg ctggtcggct tcctgactgt
      901 gctgggcatc gccgccagga acgggatcat gatgatcacc cacttccagc acctggagcg
      961 cgaggaaggc atcccgttcg gacccgagct cgtcctccgg ggcgcacagg agcgactctc
     1021 gccgatcctg atgaccgcgc tggccacggg cctggccctc gtgccgctgg tctgggccgg
     1081 gtcgatcccg ggtcacgaga tcgagcatcc gatggccgtc gtcatcctgg gcggcctggt
     1141 gacctcgacc ctgctgaacc tgttcctggt gccgtcgctc tacctgcggt tcggcaagtc
     1201 tgaccgcggc acggaaacgc cgtccggtcc ggaactcgtc gcggcctgat cgactggcca
     1261 tctccacact acacggtctg cgccgtcctc cgctcggacg gcgcagaccg tgtccgccgg
     1321 ccgaatcagc tcccgaatcc ccgcacgacg tcgacatcgg ctttcgcacc cgtgaagatc
     1381 aggttgacgt ccgtgttggt ggcgaagaat cgaccgccgg cgtcgtacca gcgctggaac
     1441 cggcgcgcgt cccgctcgac catcccgcag accttgccag cggcggttac gcgctccatg
     1501 acgtgcgtta ccgtctcgac gaatgtcggg ttgtcgtact ggccgggcat cccgagcgag
     1561 atcgacaggt cctgcggccc gacgcagatc acgtctacgc catcgaccgc cgcgagctca
     1621 tcgatccgct cgaccgcctg tgtggtttcg atctggacca cgaccacagt cgtggcgttg
     1681 acttcggtca ccccatcagc cggcgtgcgc cgctcgaatc catatcgccc ctccccaccg
     1741 gcgcctcgcc ggccgatcgg ggggtagtag gcgtattgga cagccgcttc aacttgctcg
     1801 accgtttcga cccgcggcac gattacgccg attgcgccgg aatcgagcgc ccgggcgacg
     1861 agcggatagg agagatcggt cgcgcgcacc agcggcgtga tcccgacggc gcgggcgtac
     1921 tggaccagtg tctggatcgt ctcccagccg aacatggcgt gctccatgtc cagccagacg
     1981 aagtcgtgcc cggcgttcgc cagcgccttg atcaggccga ccgtccggat ctcgctgacg
     2041 atcggtccgc tgactgcctg tccgctccgc agcttctgct tgaaatccgc cacctattcc
     2101 cccgatcccg ataagcgctg caccattgtg atcaacgccg aatgatccaa ttgtccgccg
     2161 cccgtcgcgg agagcgcgtc aaagagctgg ctgaccagcg cggtcaccag cagggccgac
     2221 tcccgttccc gaccggtcgc cagcgcgatt gcaaggtcct tgcggtgcag atcaacccgg
     2281 aagccaggct ggaactcgcc cttgatcatc gtctgaccgc gcaactccag aaccctgctg
     2341 tttgccaatc cgccgccgag cacctgcgca accttctccg ggtcgacgcc ggagcgctcg
     2401 gccaggacca gcgcctcgct gaccgccgcg tagttgatcg cgaccattac ctggttgcag
     2461 gccttggcga tctgccccgc gccaggaccg ccgatgtgga cgatggtctt gcccatcgcc
     2521 tcaaagagcg gcatcgcccg cttcaggtcc tcctcgctgc cgccgaccat gatgctgagc
     2581 gtgccggcaa tcgcgccctt gtcgccgccg ctgaccggcg cgtcgatcgc ggagccgcct
     2641 gctgtcccga ccgcggtgct gacctcgatc gcggtcgacg gggcgatggt gctcatgtca
     2701 atgagcagga agcctggccg caccgcagac agcacgccgt cgtcaccgaa cacgactgcg
     2761 cgcacgtccg gcgaatcggg cagcatcagg atgaccgtgt cggctgctcg ggctacttcc
     2821 gccggggagc ccgctgccgt aacggcatcg gactccgcag acagctcgtc gaccgcaccc
     2881 tggctgcggt tgtggatgac cagccgatag ccggccttga ccagattccg gaccatcggc
     2941 ttgcccatga tcccggtgcc gatgaacccg atcgtctgcc ccgttccctc cgccatcacg
     3001 cgtactcctt catccagccc agcgaatcct cggtccgctc ggtactgggc ttgtactcga
     3061 gtccgatcct gcctgagtag ccaaccgcat cgatcacctc gaagatcggg cgccagtcca
     3121 actccccggt acccggctcg ttccggcccg gcgcgtcggc aagctggata tggccgatcg
     3181 cgtcgatgtt ctcgcggatc gcgttgacga tgtcgccttc ggtccgcgcc gcatggtaga
     3241 tgtcgtactc gaccttcgcg ttggggtggt tcgcctcggc gacgatttcg aaaccggcgc
     3301 tcggcgttgg caagaagaag ccgggtgcgt cgtgcgggtt gagcggctcc gttaccagca
     3361 agatgccctc gacggctgtc tgatccgcca cctgccgcag attggcgatt agcgtcgcgc
     3421 gttgccgatc atcagggaca tcgccgtgcc gcttcccggc catgaggttc atcttggccg
     3481 gtttgagcgt acgcgcatac gcgagacccg tctctattgt ccacttgaag tcgtcgatgc
     3541 gagccgggtc gttggccatc ccgcgatcgc cggccgcgaa atcgcccgcc ggaaaattga
     3601 agaggacgag ttcgaggttg tgcctgtcga gctcggtctt gatttccttc gcatcgaatt
     3661 cgtaggggaa ctggaactcg accgcgtcaa atccggctgc tcgtgcccgc ccgaatcgtt
     3721 ccagaaacgg tacctccgcg aacagcatcg agacgttcgc acaaaactga accatgcctg
     3781 ccttcctccc ggacccgcgc ctgataggcc cggccccctg atacaatcga tcagttattc
     3841 atccaacgta ttcgggagcc agccttttat gaccgactca cacattctga ctgccgaaga
     3901 actggcggat cgtcgctacc cgtcagcaat cgcaatctcg aacgatggcc gcttcgtcgc
     3961 cctggtcatt gagccgatgg gccgtcgcga agagcactcc gtgagcgaaa tctggctctc
     4021 gcgcgatggc cagcctgccc ggcagttcag ctcgggactc gcgggcgacg cctctccggt
     4081 attctcaccc gattccgcca aactcgcttt cctctccgat cgccatgagc ccaagaccca
     4141 gcgtatctac ctgctcccgc tcgacggcgg cgaggcgacg cgcgtctcga cgatcgaggg
     4201 caccctcgcg agcctggcct ggtctcctga tggcgcgcac ctcagcgtgc tcgtcaccga
     4261 tcccgagacc gagatcgaga agaagcagaa ggaggagcgg aacgacccaa tcctcgagaa
     4321 ccagaatctg aaatacgccc gtctcttcgt catcgacgtt gactccggcg cgagccgcca
     4381 gctcacgttc ggcaatcgca acgtgctcga ctatgcctgg tcccgcgaca gccagacgct
     4441 cgcgatcgcc acctcgcatc gaaccgatgt cgaaggcgag ttccaggacg tcgacgtctc
     4501 gctggttgcc gtcaccggcg gacgcttgcg caccgtcgcg accctccggg gcgtcgccag
     4561 cccgctcatc ttcgtcgatc atccggatgg cgagcagctc gccatgcgac acaacggctt
     4621 cttcgacgac ccggtcgatt cagtctggct gatgtcgacc gcgaccggcg aacagcgcga
     4681 gctgctgacc gacgaccacg gcaatgtcga ctggatcggc gccgttcccg gcaagccggg
     4741 ccagatggcg atgcgcatgg tcgacggcgt gcatgccaat gcctggctcg tcgacgtcac
     4801 caccggcgaa cgcacgaaga cccttcccgc gaactggcag gatcggggct cgctccacgc
     4861 tggtccggtc tattcggccg acggcaagaa gctcgccttt gtctggtcgg actcagtgac
     4921 gccggaagag gccttcgttg ccaccgtcgg caaggagccg gtcaagctca cttcattcgg
     4981 cgagtcgttc accgggcggc tgaaccggag cgaggtcgtc gagtggcaat cggacgaatg
     5041 gacgatccag ggccacctcg tctacccgca cggctacgaa gaaggcacgc gctaccccct
     5101 cctgatcgaa gcgcacggcg gcccgtcgtg gcagtgggag gatcactgct tccttgattg
     5161 gcacgactgg gcgcagttca tggcatcccg cggattcgcc gtgctggcgc cgaacccacg
     5221 cggctcgacc ggccggggcg cggcgttcca gaaagcactt cagaacgacg tcggcggtgg
     5281 cgaagtccgg gatctgatta ccggcgctca ggcgatggtc gacctgggca ttgccgatcc
     5341 cgaccggctc gggatcggtg gctggagctg gggcggctac ctgacggcga ccaccatcac
     5401 gcgcaccaac atcttcaagg ccgcaatgat gggggccggg ctctcgaacc ttttctccga
     5461 tcacggcacc gacgacatcc cgtcggccaa cctgctctat ttcccgggcg agccgtacca
     5521 ccacgccgac aggtactggg ccggatccgc cctcaagtgc gtcgccaact gcacgacacc
     5581 gacgttgatc atccacggcg acgccgacga gcgggtccac ccgttgcagg gcaaggagat
     5641 gtacaccgcg ctctcccggc ttgggattga cacggagttc gtccgctacc cgcgccaagg
     5701 ccacacgatc aaggagcgca actaccagat tgacctgctg aaccggttgg ccgcctggtt
     5761 cgagaagtac ttgatgacct agccggcgcc gagcagacgc tcgatcgtga gcgcgtagat
     5821 ctttgcctgg gcaacgaggt cgtcgatata gacagcctcg ttcgctccgt gggcattctc
     5881 accgccggga cccatcccga ccgtgtcgat cccggccagg cggtaatacc ggccatcggt
     5941 cgcgccggcc cactggacga agaattctgg ctccttgccg cgcacctctc gggtggcgtc
     6001 ccatacggac tgaaccagcg gcgagtcggc gtcggtgaga ttggccggga tgaaatcccc
     6061 gtccggatca actaccaatg agtagcggaa ctcgcccggt tcggccgcgg ccacattcgc
     6121 cagggtctcg gtgatctcgt tgacgaccga ctcccgcgtc tcgcccggga tcaggcggcg
     6181 atcgatcgtg aagacgcact cgccaggcac gacgttgtgc gcaacgccgc cagaaatctg
     6241 gttgatgctg atcaccggct tgccgaccga cgggtggtag cggtcgatgt ccgtctccag
     6301 ggccaggatc gccttcgcgg ccagcgcgat cgcgttgacg ccggtcgtgc gagcggccgc
     6361 agtgtgggag gcgcggccat ggaccgtgac ccgcataccg aggatgccgc gctcggcgtt
     6421 gcgcaccttg aggtcgctct tctcgccgat caagcagtag gtcggcttcg gcagcgtgcc
     6481 gtcggcgatg gcgtccatca ccgcaaccgt gccgtaatgc ccgctcagct cttcgtcgct
     6541 gacgatgtgg gcttgcaggc agccgcacat cggccgatcc cggaacagct ccatcatcgc
     6601 cagcatcacg ccgacggatg ccttcatgtc agaagcaccg cgaccggaga gccgcattcc
     6661 gtcttcagag atgacgggat cgaaagggtc ggtcgtccac ttgtcggcgt ccgaaaccgg
     6721 cacggtgtcg aggtggccgt tcatggcgag cgtcgaaccg gaggccgggt cgccgatgct
     6781 gatgatcagg ttcgggcgga gctcgtcgcg cgcaatcgtc gtgacggcga gttcacgcgc
     6841 gcggcaccag tcgtgcaccc actgcatgat caccgcttca tcgccggaaa cgctgggaat
     6901 ggcgatcagc gatcgggcaa tcccaagca
//
LOCUS       DASSGX010000559         2722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1188567, whole genome shotgun sequence.
ACCESSION   DASSGX010000559 DASSGX010000000
VERSION     DASSGX010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188567"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /locus_tag="VFI12_10130"
     CDS             <1..1213
                     /locus_tag="VFI12_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056810.1"
                     /translation="LIVRRLLEGKRVMHTAQDRKLPQEVFTVVAEFMWEHHAKLFPQR
                     NGRATKPRYANGQEEVILRNGGVYSIVAPTRGGARGPSRDLVIVDELREMDTWDFIAA
                     AKPTLTASKDPQMIYLSNAGADLSVVLNKLRERRDADPSLAYLEWSAPPDLRPDDVEG
                     WAAANPGMGHETGEMGSIYETLVDDYRTAALENTLAIFETEHLCRWVDTMREPFVEPG
                     AWAANEANQPTDPHRPYMAISMDPGGKRAAAAIAWPRSDDKMGFRLLFDVTGNPINVD
                     KLGPDMAAMAQELHVPLVGFDPLTDAVLAKAFRKTEKLTGAVFANACADFVQRVEGQK
                     IAWDHAAAVGEDLQWTARKLHDDSGSFQAVRADDNRPIPAALAAIRAVGLAARPSPRS
                     VYEDRGVLALG"
     gene            1210..1368
                     /locus_tag="VFI12_10135"
     CDS             1210..1368
                     /locus_tag="VFI12_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056811.1"
                     /translation="MKTLDSDQALALTGLSLLAIGLALVAVPLSLSVIGLLLIGYSIA
                     PDHGEKKP"
     gene            1365..>2722
                     /locus_tag="VFI12_10140"
     CDS             1365..>2722
                     /locus_tag="VFI12_10140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HET7056812.1"
                     /translation="MKTLIPARSTKAIDQQAMAGIDNVTYLPLENLRTWTGLGVSPET
                     AMNMVTVYQCVRVLANAYAQIPLLFYRRLGDSRKERATDHPLYETFHLRPNPDMTSFA
                     WRRLMMTHLATWGNAYCEVTRDLLGRIQLWPIRPDRIEPKFDSDGRKVYDYLSPLGER
                     KPLEPGRIFHIAGLSRDGLKGVSPITELRSTIGLGKTAERFGESFFRNNARPATVLQH
                     PMTLSEGAVTRLAAQMEDLKGSANAGKTVVLEEGTTIQEIGIPPDDAQFMETRLFQKR
                     ELAGAYGIQPHKIGDLERATFSNIEQQSLEFIQDTMMPWFVLTEQEIAAQLLPDGDNS
                     YFAEFLIDGYLRADAKTRAESFAIRWQHGALSADEWRAKENENPIEGGIGERFYVPVN
                     YVAVEADDPVAQPAAEPQQPPQLSVVKSAAVRCDKGHLLAELATPPYRFTCSKCKSVV
                     ST"
BASE COUNT          492 a          928 c          875 g          427 t
ORIGIN      
        1 gctgatcgtg aggcgcctcc tcgaaggcaa gcgagtcatg cacaccgcgc aggaccggaa
       61 gctgccccag gaagtcttca cggtcgtggc cgagttcatg tgggagcacc acgccaagct
      121 cttcccgcag cgaaacggcc gggccacgaa gccccgctac gccaacggcc aggaggaagt
      181 catcctccgc aacggcgggg tctacagcat cgtcgccccg acgagaggcg gagcgcgcgg
      241 accctcccgc gatctcgtga ttgtcgacga gctgcgcgag atggacacct gggacttcat
      301 cgccgcggcc aaaccgaccc tcaccgcgtc gaaggacccg cagatgatct acctctcaaa
      361 cgcgggagcc gacctgagcg ttgtcctcaa caagctccgg gaacgccgag acgctgatcc
      421 atcgctcgcc tacctcgaat ggtcagctcc gcctgatctc cgtcctgatg acgtcgaggg
      481 ctgggctgcg gccaatccgg ggatgggcca cgagaccggc gagatgggct cgatctacga
      541 gacgctcgtc gacgactacc gcaccgcggc gctggagaac accttggcga tcttcgagac
      601 cgagcacctg tgccgctggg tcgacacgat gcgcgagccg ttcgtcgagc cgggtgcgtg
      661 ggcagccaac gaggcgaatc agccgaccga tccgcaccgg ccgtacatgg cgatcagcat
      721 ggatcccggc ggcaagcgtg ccgcggccgc gatcgcctgg ccgcgctcag acgacaagat
      781 gggtttccgg ctcctcttcg acgtgaccgg caaccccatc aacgtcgaca agctgggtcc
      841 ggatatggcc gcaatggcgc aggagctcca cgtccccctg gtggggttcg acccgttaac
      901 cgatgcggtc ctcgccaagg cgttccgcaa gaccgagaag ctgacgggcg cggtgttcgc
      961 gaacgcctgt gccgacttcg tgcagcgcgt cgaagggcag aagatcgctt gggatcatgc
     1021 ggccgcagtt ggcgaggatc tgcagtggac cgcccgaaag ctgcacgacg actcgggctc
     1081 gttccaagcg gttcgggctg acgacaaccg cccgatcccc gcagccctgg ccgcgatccg
     1141 tgcggtcggc ctcgctgccc gcccatctcc gcgatctgtc tacgaagacc gcggagtcct
     1201 ggcgctggga tgaagacgct cgacagcgat caggcgctcg ccctgaccgg tctcagcctg
     1261 ctcgcgatcg gcctcgccct cgtcgcggtg ccgttgtccc tgtccgtcat tggcctgctc
     1321 ctcatcggat attccatcgc gcctgaccac ggggagaaga agccatgaag acactgatcc
     1381 ccgcgcgctc cacgaaggcg atcgatcagc aggcgatggc cggtatcgac aacgtcacct
     1441 acctgccgct cgagaacctc cggacgtgga ccggtctcgg ggtgtcgcct gagacggcga
     1501 tgaacatggt gaccgtctac cagtgcgtcc gtgtcctcgc gaacgcctac gctcagatcc
     1561 cgctgctctt ctaccgccgg ctgggtgact cgcggaagga acgagcgacc gaccacccgc
     1621 tctacgagac gttccacctt cgccccaatc cggacatgac ctcattcgcc tggcggcggc
     1681 tgatgatgac ccacctcgcg acgtggggga acgcctactg cgaggtgact cgggaccttc
     1741 tggggcggat ccagctctgg ccgattcgcc cggaccggat cgagccgaag ttcgactccg
     1801 acggccgcaa ggtctacgac tacctgtcac cccttggcga gaggaagccg ttggagccgg
     1861 gtcggatctt ccatatcgcc gggctgtcgc gggatggctt gaagggcgtc tccccgatca
     1921 cggagctccg gtcgacgatc gggttgggca agacggctga gcggttcggc gagtcgttct
     1981 tccgcaacaa tgcgcggccc gccacggtcc tccagcaccc gatgacgctg tccgagggtg
     2041 ccgtcacccg tctcgcggcc cagatggaag acctcaaggg ctcggcaaac gccggcaaga
     2101 cggtggtgct cgaagagggc acgacgatcc aggagatcgg catcccgccc gacgacgcgc
     2161 agttcatgga gacgcgcttg ttccagaagc gcgaactggc cggcgcgtac ggaatccagc
     2221 cccacaagat cggcgacctg gagcgggcga cgttctccaa catcgagcag cagtcgctcg
     2281 agttcatcca ggacacgatg atgccgtggt tcgtcctgac cgagcaggag atcgcggccc
     2341 agctgctacc tgacggggac aactcgtact tcgccgagtt cctcatcgac ggctacctcc
     2401 gggctgacgc caagacgcga gcggagtcgt tcgcgattcg ctggcagcac ggcgccctgt
     2461 cagctgacga atggcgggcc aaggagaacg agaacccgat cgagggcggg atcggagagc
     2521 ggttctacgt ccccgtcaac tacgtcgcgg tcgaggcgga cgatcccgta gcccagcccg
     2581 ccgccgagcc gcagcagccg ccgcagctct cggtggtgaa gtcggccgcc gttcggtgcg
     2641 acaagggtca cctgctcgcc gaactcgcga ccccgcccta ccggttcacc tgctcgaagt
     2701 gcaagagcgt ggtctcgacg ga
//
LOCUS       DASSGX010000560         2782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_206713, whole genome shotgun sequence.
ACCESSION   DASSGX010000560 DASSGX010000000
VERSION     DASSGX010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206713"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="VFI12_10145"
     CDS             <1..858
                     /locus_tag="VFI12_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HET7056813.1"
                     /translation="YGGQSMTATLRTVMVRQPAPTVGGDEWQSFGYTRPIRPAETARE
                     HREFVDLLRANGVEVVEVGPDPVGHLDAIFAYDPSLMTNRGAILLRPGKDLRRDEPAL
                     HRATYAELGIPIAGEIEAPGTVEGGDTLWLDERTLAVGRGYRTNAEGIRQLTEILRAM
                     GVQVLVYDLPHWNGSSECLHLMSMISPVAEKVAVVSMRMMPVALVEWLKENGWTLIEM
                     PESEFDSMGCNVLALGEGRVVIANQNPETSARLQAAGLTVLEYQGDHISHNRQGGPTC
                     LTRPILRAS"
     gene            935..2098
                     /locus_tag="VFI12_10150"
     CDS             935..2098
                     /locus_tag="VFI12_10150"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET7056814.1"
                     /translation="MKRETDSATRFSTDELLNLEPSAVMELHKQGRITRRDVFKVMGA
                     AGVATFGMRLLDDSALAKEELKMIIWEGYADEKYRVPFEKANDATVSFTEAGTGDEMF
                     AQMKDSDGKNYDMVSASSDLPKRLYDAGLLAEIDTSKLTNYNDLWDQFKTPDYITFDS
                     KLYGVNFAWGPTLIISNADEVKTAPTSWSALFDEQYKGKISTWNYPLQIAQYAQLLDP
                     VPEDVYVLTDEQLAQVKDMLVKQRPLVRKYWDTGAELSQLVLNKEVVIADGWTWITNQ
                     LKQGGANVVESIPTEGVTGWSDSWVISKAAKNYDLALKWADWMIGPEGQVGLTEVMGY
                     SITNKKAAASLPEDVQKNLRLTDVADQYAQIKMWKFVENYDKWVQVWNEATQG"
     gene            2177..>2782
                     /locus_tag="VFI12_10155"
     CDS             2177..>2782
                     /locus_tag="VFI12_10155"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7056815.1"
                     /translation="MATEAVQTSAPARKRSRWLAIAGLLSAPVGWMVVFYLIPIGILL
                     VHAFWSVKYPEIDHTLTLKNFRTILNSDLYVRVFVRTILMAAAVTLADVVLAFPLAFW
                     LAKRVRRNRELLLFLVIFPLWSSYLIRIFAWKTLLGTTGILNSFLLWAGIVNEPLSFF
                     LYSKWSIFLALTHVWLPFMILPLFTVLDRVPNSLLEASSDLG"
BASE COUNT          532 a          851 c          883 g          516 t
ORIGIN      
        1 tacggcggcc agtcgatgac ggcgacgctg cgcacggtca tggtccggca accggcgcca
       61 accgtcggcg gggacgagtg gcagtccttc ggatacaccc gtccgatccg ccctgcggag
      121 acggcccgcg agcaccgtga gttcgtcgat ctgcttcgcg ccaatggggt cgaggtcgtg
      181 gaggtcggac cggatccggt cggccatctc gatgcgatct tcgcctacga cccgtcgctg
      241 atgacgaacc gtggcgcgat tctgctccgg ccgggcaagg atctgcgcag ggacgagcca
      301 gcgctgcacc gggcgacgta tgccgagctc ggcatcccga tcgctggtga aatcgaggcg
      361 ccgggcacgg tcgagggcgg tgacaccctt tggctggacg agcgcacgct ggccgtcggc
      421 cgcggatacc gaaccaatgc cgaggggatt cgccagttga ccgagatcct gcgggcgatg
      481 ggcgttcagg tgctggtcta cgacctgccc cactggaacg ggtcgagtga gtgtctgcac
      541 ctgatgtcga tgatcagccc tgtggctgaa aaggtcgcgg tcgtctccat gcggatgatg
      601 ccggtggcgc tcgtggaatg gctcaaggag aacggctgga cgctgatcga gatgcccgag
      661 tccgaattcg actcgatggg ttgcaacgtc ctcgcattgg gcgaaggacg cgtcgtgatt
      721 gccaatcaga accccgaaac atcagcccgg ctccaggcgg ccggcttgac ggtgctcgag
      781 taccagggcg accacatctc gcacaatcgc cagggcggcc cgacctgcct gacccgccct
      841 attctgcggg ccagttgaag gcgtagccct caatacgata ttgtgccgcc tgccagaact
      901 gggcgaatcg aacataaggg cgagggagca tgcgatgaag cgtgaaactg attccgcgac
      961 ccggttttcc accgacgagc tgctcaacct cgagccgtcg gccgtcatgg agttgcacaa
     1021 gcaaggccgg atcacccgga gagatgtctt caaggtgatg ggcgccgccg gcgtggctac
     1081 cttcggcatg cgtctgctcg atgacagcgc cctggccaag gaagagctca agatgatcat
     1141 ctgggagggc tacgcggacg aaaagtaccg cgttcccttc gagaaggcga acgacgcgac
     1201 ggtcagcttc accgaggccg gcaccggcga cgagatgttc gcccagatga aggacagcga
     1261 cggcaagaac tacgatatgg tctcggcctc gtcggacctg ccgaagcggc tctacgacgc
     1321 cggcctcctc gccgagatcg acacgagcaa gctgaccaac tacaacgacc tctgggatca
     1381 gttcaagacc ccggattaca tcacgttcga cagcaagctc tacggcgtga acttcgcctg
     1441 gggtccgacg ctgatcatct cgaacgcaga cgaggtcaag acggcgccga cctcctggag
     1501 cgcgctcttc gacgagcagt acaagggcaa gatctcgacc tggaactacc cactccagat
     1561 cgcccaatat gcccaattgc tcgacccggt tccggaagac gtctacgttc tcaccgatga
     1621 gcagctcgcg caagtcaagg acatgctggt caaacagcgt ccgctcgttc gcaagtattg
     1681 ggacaccggc gccgagctct cgcagctggt cctcaacaag gaagtggtga tcgcggacgg
     1741 ctggacctgg atcacgaacc aacttaagca ggggggcgcg aatgtcgtcg agtcgatccc
     1801 gaccgagggt gtgaccggct ggtccgactc gtgggtgatc tccaaggcgg ccaagaacta
     1861 cgacctcgcg ctcaagtggg cggactggat gatcggtcct gaaggccagg tcggcttgac
     1921 cgaagtgatg ggctactcga tcaccaacaa gaaggccgcc gcgagcctgc cggaggacgt
     1981 gcagaagaat ctccgattga ccgatgttgc cgaccagtac gctcagatca agatgtggaa
     2041 gttcgtcgag aactacgaca agtgggttca ggtctggaac gaggctaccc agggctaggg
     2101 cgtttcgccc cacattgatc ttcggccggc tccgggctgg ggggccggcc gttttcccac
     2161 atgacgcgag gacgcgatgg ccacggaagc ggtacagaca agcgcgccgg cgcgaaagcg
     2221 gtcccggtgg cttgcgattg ccggtctgct atcggcgccg gtcgggtgga tggtcgtctt
     2281 ctacctgatc ccgatcggga tactgttggt ccatgccttc tggtcggtca aatatcccga
     2341 gatcgatcac acactcacgc tcaagaattt caggactatt ctgaattcgg atctctacgt
     2401 tcgcgtcttc gtgcggacaa tcctgatggc cgcggcagtg accctggccg acgttgtcct
     2461 ggcgttcccg cttgccttct ggctggccaa gcgggtgcgg cgaaaccggg agttgctgct
     2521 tttcctggtg atctttccgc tctggagctc atacctgatc cggatcttcg cctggaagac
     2581 gctgctcggc acgaccggga tcctgaactc gttcctgctt tgggcgggga tcgtgaacga
     2641 gccgctctcg ttcttcctct actcgaaatg gtcgatcttc ctggccctga cgcacgtctg
     2701 gttgccgttc atgatcctgc cactcttcac cgtgctggac cgggtcccga actcgctgct
     2761 cgaggcatcg tccgacctcg gc
//
LOCUS       DASSGX010000561         2513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_863758, whole genome shotgun sequence.
ACCESSION   DASSGX010000561 DASSGX010000000
VERSION     DASSGX010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863758"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..1801)
                     /locus_tag="VFI12_10160"
     CDS             complement(152..1801)
                     /locus_tag="VFI12_10160"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha/beta"
                     /protein_id="HET7056816.1"
                     /translation="MPDKATQTGPTHCPKCRAPLLGRTRFERFRVCDECEHHFPIEVD
                     RRIAALVDSGSFVELDRSLASTDPLLFADAEPYQDKLKQAQRAHGRGDAATYGRATLY
                     GNPIALFVLDFSFLGGSMGVVVGEKLARAAELARKEKRALVTVVGSGGARMQEGLLSL
                     LQMAKTASAVQEVKDAGLPFISVLTHPTTGGVFASFASLGDVVYAEPTALIGFAGPRV
                     AEQFLGRPLPPGSHSAEFLLAHGFVDSVVPRHHLRSAIALTLNTLGTNVTRPKRTKGT
                     TGHLEPLEPWEAVETVRDPDRPTALHYITECLDYWLELHGDRTGTDDRAVLAGLGQLE
                     GQAVAVIGFERDSDGDPIDRRGGRPMPGGYRKAQRLMRLASRFRIPLIAFVDTPGAYP
                     GIESEEEGLAFEIATTMAAMIRIDAPTVSVIVGEGGSGGALAFAVADRVLMQERAIYS
                     VIAPEGAAAILYRDASRAPELADRLKITASEVRKLRISDGTIPEPQGGAKADPAVAAK
                     RVKTAILDHLAELNRMDRDKRKADRYRRYRRIGTNYLTTIE"
     gene            1943..2224
                     /locus_tag="VFI12_10165"
     CDS             1943..2224
                     /locus_tag="VFI12_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056817.1"
                     /translation="MSPDFEQEGVRIDVLRRLAEQAMTDLNFRSIARDDLNAALAQFG
                     YDLNPNEMSLVLKFRAVLEEAGLDLFLIGDLTDHEEDLLALAGLGGPNR"
     gene            complement(2221..>2513)
                     /gene="upp"
                     /locus_tag="VFI12_10170"
     CDS             complement(2221..>2513)
                     /gene="upp"
                     /locus_tag="VFI12_10170"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF026032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HET7056818.1"
                     /translation="NRLPEQMTDDVLYMLDPMLATAGSATATATFLKERGAKHIVFLG
                     LIAAPEGVARMAAEHPDVPIFVARLDRQLDANKYIRPGLGDAGDRLFGTVPS"
BASE COUNT          415 a          812 c          849 g          437 t
ORIGIN      
        1 cgaggtcgcg gtgatccggg acgtgctcgg tccgaccaac acgaactgga accgccccta
       61 cgagtatgag gtcgagtggc ccacctttat gcgctgctgg ggcgcgcagg gcaaggacgg
      121 catcgcggtc ggccttccgg cggagtgatc gctattcgat tgtggtgagg tagttcgtgc
      181 cgatcctgcg gtatcgccgg tatcgatccg cctttcgctt gtcccggtcc atgcgattga
      241 gctcggcgag gtggtcgagg atcgccgttt tgacccgctt ggcggcgacg gcggggtcgg
      301 ctttggcgcc accctggggc tccggtatcg tcccgtccga gatccggagc ttgcgcacct
      361 ccgacgccgt gatcttaagc cggtcggcta gctcgggcgc acggctggca tcgcggtaca
      421 gaatcgcggc cgccccctcg ggcgcgatca ccgagtagat cgcccgttcc tgcatcagga
      481 cccgatcggc tacggcgaag gcgagcgctc cgccgcttcc cccctcgccg acgatgactg
      541 ataccgtcgg cgcatcgatc cgaatcatcg cggccatcgt tgtggcgatc tcaaaggcca
      601 gcccctcttc ctccgactcg atgccggggt acgcgccggg cgtgtccacg aacgcgatca
      661 gcgggatccg gaaacgggac gcgaggcgca tcagacgctg cgctttccga tagccgccgg
      721 gcatcggacg cccgccgcgg cggtcgatcg ggtccccgtc cgaatcgcgc tcgaatccga
      781 tcacggcgac ggcctgacct tcgagttggc ccagcccagc gagcaccgcc cggtcgtcgg
      841 tgccggtgcg gtcgccgtgc agctccagcc agtagtcgag gcattcggtg atgtagtgaa
      901 gcgcggttgg ccgatccgga tcgcggacgg tctcgaccgc ctcccatggt tcgagcggct
      961 ccaggtggcc agtggtcccc ttggtgcgct tgggccgggt gacgttggtg ccgagggtat
     1021 tcagcgtcag ggcgatggca gagcggaggt gatgacgcgg gacgacggaa tcgacgaacc
     1081 cgtgcgcgag caggaactca gccgagtgac tacccggcgg caggggccgg ccgaggaatt
     1141 gctcggcgac gcgcggaccg gcgaacccga tcagggccgt cggttcggcg tagacgacat
     1201 cacccaggct ggcgaatgac gcgaagaccc cgccggtcgt cggatgagtc aggaccgaga
     1261 tgaagggtag gccggcatcc ttaacctcct gcacggcaga ggcggtcttc gccatctgga
     1321 ggagcgagag cagcccctcc tgcatccggg cgccgcctga cccgacgacc gtcacgagcg
     1381 cccgcttctc cttgcgggcg agctctgcgg cgcgggccag cttctcgccg acgaccacgc
     1441 ccatgctgcc gccgagaaag ctgaaatcga gcacgaagag cgcgatcggg ttgccataga
     1501 gggtggcgcg gccataggtc gcagcatccc cgcggccatg cgcgcgctgt gcttgcttca
     1561 gcttatcctg gtacggttcg gcgtccgcga aaagcagggg atcggttgat gccagcgaac
     1621 gatcgagctc gacgaagctt ccggagtcca caagggcggc aatccgccgg tcaacttcaa
     1681 tcgggaagtg gtgctcgcac tcgtcgcaga cccggaatcg ctcaaatcgc gtgcgcccaa
     1741 gcaggggcgc ccggcatttt gggcaatgcg tcggtccggt ttgcgtcgcc ttgtctggca
     1801 ctgcatctcg ctccatcgtg cgtccgaaac gggacatttg agccccgaat aatggtagtg
     1861 ccggttcgca acaggtgtac aatcgagtcg atccccggtc cgcgcatgtg cgctcggggt
     1921 gcgcaagatt gcgaaggttt ccatgtcacc agatttcgag caggaaggtg tccgcatcga
     1981 tgtcctgcgc cgcttggcgg aacaggcgat gaccgatctc aattttcggt cgatcgcgcg
     2041 ggacgatctg aacgcggccc ttgcgcaatt tggatatgac ctcaatccca acgagatgag
     2101 cctggtcctg aaattccgcg cggtgctgga agaggccgga ctggacctct tcctgatcgg
     2161 cgacctcacg gaccacgagg aagatctcct ggcactcgcc ggcctgggcg gtccgaaccg
     2221 ctaggacggc accgttccga agagccgatc ccccgcatcg cccaggcccg ggcgaatgta
     2281 cttgttcgcg tcgagctggc ggtcgagccg cgcgacgaag atcggcacgt ccgggtgctc
     2341 cgcagccatt cgcgcgacac cctccggcgc tgcgatcaac cccagaaaga cgatgtgctt
     2401 cgcgcctcgc tccttcagaa aggtggcggt cgcggtggcc gagccggctg tggccagcat
     2461 cgggtcgagc atgtagagca cgtcgtcggt catctgctcc ggcagccggt tgt
//
LOCUS       DASSGX010000562         3447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_863852, whole genome shotgun sequence.
ACCESSION   DASSGX010000562 DASSGX010000000
VERSION     DASSGX010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863852"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VFI12_10175"
     CDS             <1..375
                     /locus_tag="VFI12_10175"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HET7056819.1"
                     /translation="VFLWKLVGIVALGNLLLAGLSFWDTGVVPGLYDPTVLVRAGTTV
                     TVTAELLNVRAEPDADSAVIDQVGQATELRVTGIAEGGWWPVTYDHDGQPVEGWVSAE
                     HVEGLPQSGYQRVRRALRELIP"
     gene            430..1137
                     /locus_tag="VFI12_10180"
     CDS             430..1137
                     /locus_tag="VFI12_10180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET7056820.1"
                     /translation="MTDKTQDRSSADDVGFALRVRQGDHDAFRALYDRYGSAVLGLAQ
                     SVVHDRAAAEDITHDVFFGFWRNPYAFEPARGQFVAWLLRVTRNRAIDVLRKRRDVSF
                     GNARSAQDGEAVDPTQWIADPEPGPETEAVTHTLADDVRAALRTLPDDHRQLLELAYF
                     GGLTQREIAERLHRPLGTVKTQMRTSLMKLSRLASVRELATSMDLDRIVVEERQDSGS
                     WSLTSANPAESVGPETS"
     gene            1152..1928
                     /locus_tag="VFI12_10185"
     CDS             1152..1928
                     /locus_tag="VFI12_10185"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HET7056821.1"
                     /translation="MKSERSNEMCIEIADLIEGYAIGALDEPEMLLVADRIGECPEQQ
                     ERLLQYEETVGLLGLVTAPIEPSVALWERLDRSTTNVALPEPIPLHATDRRRLVVPNW
                     LAMAAAAAAILLLISTVSLGVALHRANSDDNSQAFESTVSTYLTSGGQVVPLTSLKTP
                     DSLGWAGQGSLLIAPNMPPMIVVDKCKPSSKSYVYVVWLQRGDQRTPMGTIDIGQDGR
                     GMMMLEGVDSLAGYDVLGITIHTGNDWTYDVMTGSPTQKN"
     gene            complement(2466..>3447)
                     /locus_tag="VFI12_10190"
     CDS             complement(2466..>3447)
                     /locus_tag="VFI12_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7056822.1"
                     /translation="KQVQAMRGFARRYKIIARRGSYHGMTYGAMSLTALKNEHHFGPF
                     MYGVSHVPHPNKYRSDFGVEGEQADIMAAKAVEQEIMFQGPDTVAAVIGEPISTAAGV
                     HVPSRIYWQMLREICDKHGVLLIADEVINGFGRTGTMFAIEQYGTVPDIMTIAKALSS
                     GYAPISAAIIRDSVFEIFKEKQEVPFGHLLTFGGHPVAAAAAIKNLEIFDEENLVEKG
                     AEMGAYLGKRLEELRAHPTVGDVRGVGMVWALDLVKSKKDKTVWGRDHGFIRTVQDVS
                     MANGVITRTWDVMHFAPPLVITKEEVDRMVAAADEALTAAEKEFAGEITD"
BASE COUNT          604 a         1079 c         1126 g          638 t
ORIGIN      
        1 gtcttcctct ggaagctggt cggaatcgtc gcccttggca atctcctgtt agccggactt
       61 tcgttctggg atacgggcgt agtccctgga ctttacgatc cgaccgtcct ggttcgtgct
      121 ggcacgacgg tgactgttac cgctgagttg ctgaacgtgc gggccgagcc ggacgccgac
      181 agtgccgtga tcgaccaggt tggccaagcg accgaattgc gcgtcaccgg aattgccgag
      241 ggtgggtggt ggccggtaac gtatgatcat gatggacaac ccgtagaggg gtgggtgtcc
      301 gccgagcacg ttgagggact cccccagtcg gggtatcagc gggtgcggcg cgcgctaagg
      361 gagctgattc cataatcccc ggcatgctgt cacacgtagg gtggtggggc ggggatatca
      421 gggagcgtct tgaccgataa gacccaggat cgctcatctg cagacgatgt cgggtttgcc
      481 ctgcgtgtgc gccagggcga tcacgatgcc ttccgtgcgc tctacgatcg ctacggaagc
      541 gcggtgctcg gtcttgccca gtcagtggtg cacgatcgcg cggccgccga ggatattacc
      601 cacgacgtat tcttcggctt ctggcggaat ccctatgcat tcgaaccggc gcgtggccag
      661 ttcgttgcct ggctgctgcg ggtaacgcgg aaccgcgcga tcgatgtgct gcggaagcgg
      721 cgggacgtct cattcggcaa cgcgcgatcg gctcaggacg gcgaggcggt cgacccgacc
      781 cagtggatcg cggatccaga gcccggcccg gagaccgagg ccgtgaccca caccctcgcc
      841 gatgacgtgc gggctgcgct ccggaccttg cctgacgatc atcgccagtt gctggagctg
      901 gcctattttg gtggcttgac gcaacgagag atcgcggagc gcctccaccg tcccctggga
      961 acggtcaaga cccagatgcg gacgtccctg atgaaactta gccggcttgc atcggtgcgc
     1021 gagctggcaa catccatgga cctggacaga atcgtagtag aggagaggca ggattccggg
     1081 agctggagtc tgacctcggc gaatcccgcc gaatcggtcg gcccggaaac ctcctgagta
     1141 cctgagcggt catgaagtct gaacgttcca acgagatgtg cattgagatt gcggacctca
     1201 tcgaggggta cgccatcggc gcacttgatg agccggagat gctgctcgtg gccgaccgca
     1261 tcggcgagtg ccccgagcag caggagcgac tcctgcaata cgaagaaacg gtaggtctgc
     1321 tcgggctcgt cacggctccg atcgaaccct ccgtggcgct ctgggagcgg ctggaccggt
     1381 ctacgaccaa cgtcgcgctg ccggagccga ttcccctgca tgccaccgac cgacgcaggc
     1441 tcgtcgttcc gaattggctc gccatggctg ctgccgcggc ggctatcctg ctcctgattt
     1501 cgacggttag tctcggggtg gcgctgcacc gggcgaattc cgatgacaac tcgcaggcgt
     1561 tcgaatcgac cgtctcgacg tatctgacat ccggtgggca agtcgtcccg ttgacatcgc
     1621 tgaaaacgcc ggattcactg ggctgggccg gacagggaag cctccttatc gcgccgaaca
     1681 tgccgccgat gatcgtcgtc gacaagtgca agccgagcag caagagttac gtatacgttg
     1741 tctggctcca gcgcggagat cagcgcacgc cgatgggaac gatcgatatc gggcaggacg
     1801 gccgcgggat gatgatgctc gagggtgtcg actccctcgc ggggtacgac gtgctcggta
     1861 tcacgatcca caccggcaac gactggacct acgacgtgat gaccggatcg ccgacacaga
     1921 aaaattagcg tcactcacca taagatgcgc tataattaat cgtggccgca gccgcgagcg
     1981 cgaccccaat gcggccacag acgatagacg taacgaccga ggttcttgtt gaccgatcga
     2041 aatgtgatct gcgccgcgtg ccaacgcgag ttcgtaatta ccccctctga gcgcctcttc
     2101 tacgccgaac gcggatacca ggagcccaat tactgtccgg attgccgggc caggcgtcgg
     2161 gcggatcggc acgccgagct gctcgccgtg gcgggtcaag ctcaggcggt ttcccggctt
     2221 ggcacctacg gtgggattgc gccgccggct ccgcgcggtt ccgaggcgac gagccggttc
     2281 accgcgacct gcgcctcgtg cggcggagag gcggtcgtcc cgtttcaacc gcggccgggc
     2341 cggccagtct actgccggaa gtgctttggc tcgcgccgga gccaccatgc gaacggaaac
     2401 gggcgcgggt aacgaaaagg gccggggaga tcgctccccg gccctgagta tttccggcgc
     2461 tttgcctaat cggtgatctc gccggcgaat tccttctcgg cggcagtcag ggcctcgtcg
     2521 gccgcggcga ccatccggtc gacctcttcc ttcgtgatca ccagcggcgg cgcgaagtgc
     2581 atcacgtccc acgtccgcgt aatcacgccg ttggccatgc tgacatcctg gaccgtgcgg
     2641 atgaagccgt ggtcccggcc ccagaccgtc ttgtccttct tgctcttgac cagatccagc
     2701 gcccaaacca tgccgacgcc gcggacatcg ccgaccgtcg ggtgcgcccg cagctcctcg
     2761 aggcgcttgc cgaggtacgc gcccatctcg gcgcccttct cgaccaggtt ctcctcatcg
     2821 aagatctcga gattcttgat cgcagccgcg gcagctaccg ggtgcccgcc gaaggtgagc
     2881 aggtggccga acggaacctc ctgcttctcc ttgaagatct cgaagaccga gtcccggatg
     2941 atcgcggccg agatcggcgc gtagccggat gagagcgcct tggcgatggt catgatgtcg
     3001 gggacagtgc cgtactgctc gatcgcgaac atcgtgccgg tccgaccgaa gccgttgatg
     3061 acctcgtcgg cgatcagcag gacgccgtgc ttgtcgcaga tctcgcgcag catctgccag
     3121 tagatcctgg acggcacatg gacgccggcc gcggtcgaga tcggctcgcc gatgaccgcg
     3181 gcaaccgtat ccgggccctg gaacatgatc tcctgctcga cggccttcgc ggccatgatg
     3241 tcggcctgct cgccttcgac gccgaaatcc gagcggtact tgttcggatg cggaacatgc
     3301 gagacgccgt acatgaacgg gccgaagtgg tgctcattct tgagggcggt gaggctcatc
     3361 gcgccgtagg tcatgccgtg gtacgagccc cggcgggcga tgatcttgta gcggcgggcg
     3421 aagccgcgca tcgcctgaac ctgcttc
//
LOCUS       DASSGX010000563         3812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_426413, whole genome shotgun sequence.
ACCESSION   DASSGX010000563 DASSGX010000000
VERSION     DASSGX010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426413"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VFI12_10195"
     CDS             <1..479
                     /locus_tag="VFI12_10195"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641521.1"
                     /note="catalyzes the formation of L-tryptophan from
                     L-serine and 1-(indol-3-yl)glycerol 3-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HET7056823.1"
                     /translation="AIGIFHPFAGDEGVELIGVEAGGHGIDSGAHASTLVAGREGILH
                     GSRSYVLQDEWGQVIETHSISAGLDYPGVGPEHAYLKDIGRARYVSITDDEALAGFQQ
                     LCRTEGIIPALESSHAVAYALKLAAERPKDEALLVNLSGRGDKDLNTVAEAFGVTL"
     gene            476..1318
                     /gene="trpA"
                     /locus_tag="VFI12_10200"
     CDS             476..1318
                     /gene="trpA"
                     /locus_tag="VFI12_10200"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HET7056824.1"
                     /translation="MSANVAASTRIQETFDRAKAENRAAMMPFVTAGYPSLEISGQLL
                     DALVEGGADIIELGVPFSDPLADGATVQASSQKALENGTTLADCIELVRTFRTRGGTI
                     PILLMGYVNPFYQYGIERLAREAAEAGIDGFLIPDLPSDESEEFQAPLRAQGLDLIYF
                     LAPTSTERRIADVASRATGFLYCVALTGVTGARDQMAEELPEYIAGIRQATDLPLTVG
                     FGISKPEHVAAVAQFADGVVVASALINHLDTLPTEDQPDGARSFVRQLAAATHRAPHE
                     TSRG"
     gene            1326..1769
                     /gene="aroH"
                     /locus_tag="VFI12_10205"
     CDS             1326..1769
                     /gene="aroH"
                     /locus_tag="VFI12_10205"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HET7056825.1"
                     /translation="MSASDGAGNWACRGIRGATTAIANTAEDILEATDELMRTVIALN
                     EIDPANVASVIFTTTPDLTATFPALAARDIGWTEVPLMCAHEMAVPGSLQQCVRVMIH
                     VNTTKSAAEIRHVYLKGARELRPEWGFSDDDVAGILRNQPVEVGQ"
     gene            1766..2803
                     /gene="aroF"
                     /locus_tag="VFI12_10210"
     CDS             1766..2803
                     /gene="aroF"
                     /locus_tag="VFI12_10210"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET7056826.1"
                     /translation="MIVVMRPTANKQQISAVVERIESIGGLAAHLSQGEERTIIGVVG
                     SLPPTLEEMLEVFDGVEKIVRVSSKIKLANWEFHPRKTKIKILDFEIGGDEVTIIAGP
                     CSVESEEQTMATARAVKAAGAKMLRGGAFKPRTSPYEFRGLGRHGLEILAQAREETGL
                     GVITEVMTPADVEQVYEYTDIFQIGARNSQNYYLLEEVGKARKPVMLKRGLSMLIEEW
                     LLAAEYVLSQGNPDVILCERGIRTFEKETRNTLDLAAVPVAQRMSHLPVFVDPSHAAG
                     KRPYIPALALGAVAAGADGLMIEVHPNPDQALSDGMQSLTFAEFAELMPRISAVANAI
                     GKSLPIHEPVG"
     gene            2806..>3812
                     /locus_tag="VFI12_10215"
     CDS             2806..>3812
                     /locus_tag="VFI12_10215"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET7056827.1"
                     /translation="MSNAQHDVVVVGGGVEGCSIAYHLARAGVRVRLLERWQIAAAAS
                     GASAGGVRQQGRDLREFPLAFRALDRWRTLEEELNADLDYRRNGHATTCEHEADLAEL
                     AKYVLIQRSAGLDLQLVEGDELRMLIPGIAPTVIAAAYSPEDGHANPTRTTQAFADAA
                     KRLGATIQCGVEATGLAMQGDRVIGVETTDGPISTGTVVLAAGAWSGEIAKSIGLNLH
                     ISPDGYQAMTTFPADPHLTQVLGSMRRLISLKQLPDGRYLLGGGWPGNFSLVAPRGET
                     IEENITRNFEAGAALIPQLGAAVIDHAWIGMDAHTHDEVAVLGEADGIDGLVLATGFS
                     G"
BASE COUNT          683 a         1278 c         1255 g          596 t
ORIGIN      
        1 atgcgatcgg catcttccat cccttcgccg gggatgaggg cgttgagctg atcggtgtcg
       61 aggccggcgg gcacgggatc gattcgggcg cgcacgcttc cacgctcgtc gctggccgcg
      121 agggcatcct gcacggctcc cgttcctacg tactgcagga tgagtggggt caagtgatcg
      181 agacccactc gatttcggcc gggctcgatt accccggcgt cggtcccgag cacgcgtacc
      241 tcaaggacat cggccgggcg cgctacgtct caatcacaga cgatgaggca ctggccgggt
      301 tccagcagct ctgccggacc gaagggatca tccccgcgct cgagtcgagc cacgcggtcg
      361 cctacgccct caagctggcg gcggagcggc cgaaggacga ggcgctgctg gtcaacctct
      421 ccggccgggg cgacaaggac ctgaatacgg tcgccgaagc cttcggggtg acgctgtgag
      481 cgcgaacgta gcggcctcaa cccgcatcca ggagacattc gaccgcgcca aggccgagaa
      541 ccgggccgcg atgatgccgt tcgtcaccgc cggttatccc tcgctcgaga tcagcggcca
      601 gctgctcgac gcgctcgtgg agggcggggc tgacattatc gagctcggcg tccccttttc
      661 cgacccactg gccgacggcg cgacggttca ggcctcaagc cagaaggcgc tcgaaaacgg
      721 cacgacattg gccgattgca tcgagctggt ccggacgttc cggacgcgcg gcggcacgat
      781 cccgattctg ctgatgggat atgtcaatcc gttttaccag tacggcatcg agcggctcgc
      841 cagggaggcg gccgaggccg gaatcgatgg attcctgatc ccggatctcc cctccgacga
      901 gagcgaggag tttcaggcgc cgctgcgggc acaaggcctc gacctgatct acttcctcgc
      961 gccgaccagc accgagcgcc gcatcgccga cgtcgccagc cgcgcgaccg gcttcctcta
     1021 ctgcgtcgcc ctgaccggcg tgaccggcgc gcgcgaccaa atggccgagg agctgcccga
     1081 gtacatcgct ggcatccggc aggcgaccga cttgccgttg acggtcggat tcggcatctc
     1141 caaaccggag catgtcgccg ccgttgccca gttcgccgat ggcgtggtgg tggcgagcgc
     1201 gctgatcaac catctcgata cactaccgac cgaggatcag cccgatggcg cgcggtcgtt
     1261 tgtccggcaa ttggccgcgg cgacccatcg agcgccgcac gagacgtcaa ggggttagtg
     1321 caactgtgag tgcgagcgac ggagccggaa actgggcgtg ccgcggaatc cgcggagcga
     1381 ccacggcgat tgccaacacg gccgaggaca tcctcgaggc caccgatgag ttgatgcgca
     1441 cggtcatcgc gctcaacgag atcgacccgg cgaatgtggc cagcgtcatc ttcacgacca
     1501 caccggacct gactgcgacc ttcccggccc tcgccgcccg cgatatcggc tggaccgagg
     1561 tgccgttgat gtgcgcgcac gagatggccg tgccgggctc gctccagcaa tgcgtgcgcg
     1621 tcatgatcca cgtcaatacg acgaagagcg cggccgagat tcgccacgtc tatttgaagg
     1681 gcgcgcgcga attgcggccc gaatggggct tctccgacga cgatgtcgcc ggtattctcc
     1741 ggaaccaacc ggtggaggtt gggcaatgat cgtcgtcatg cgccccacgg ccaataagca
     1801 gcagatcagc gccgttgtcg agcggatcga atcgatcggt ggcctcgccg cgcacctgtc
     1861 gcaaggcgag gagcggacaa tcatcggcgt ggtcggcagc ctgccgccga cgcttgagga
     1921 gatgctcgag gtcttcgacg gcgtcgaaaa gatcgtccgc gtctcgagca aaatcaagct
     1981 cgccaactgg gagttccacc cccggaaaac gaagatcaag atcctcgatt tcgagatcgg
     2041 cggggacgag gtcacgatca tcgccggtcc ctgctcagtc gaatccgagg agcagaccat
     2101 ggcgactgcc cgggccgtca aggcggccgg cgcgaagatg ctgcgaggtg gcgccttcaa
     2161 gccgcgcacc tccccctacg agttccgggg actcggcagg cacggactcg agatcctcgc
     2221 ccaggctcgg gaagagaccg gcctcggcgt gatcaccgag gtcatgacgc cggcggatgt
     2281 cgaacaggtt tacgagtaca ccgacatttt ccagatcggg gcgcgcaatt cgcagaacta
     2341 ctacctgctc gaggaggtcg gcaaagcacg caagccggtc atgctcaagc gcggcctctc
     2401 gatgctgatc gaagagtggc tgctggcggc cgaatacgtc ctctcccagg gcaacccgga
     2461 cgtgatcctc tgcgagcgcg gcatccgcac cttcgagaag gagacgcgaa acacgctcga
     2521 cctcgccgcc gtgccggttg cgcagcggat gtcgcacctg ccggtcttcg tcgatccgag
     2581 ccatgccgcc ggcaagcgcc cgtatatccc ggccctcgcg ctcggagcgg tcgcggctgg
     2641 agccgacggg ctgatgatcg aggttcaccc gaaccccgat caggcgcttt cggacggcat
     2701 gcaatcgctc accttcgccg agttcgcgga attgatgcca cgcatctcgg ccgtcgccaa
     2761 cgccatcggc aaatcgctcc cgatccacga acccgtcggc tagccatgag caacgcacag
     2821 cacgacgtcg tagtcgtcgg gggcggagtc gagggctgct cgatcgcgta ccacctggcc
     2881 cgggccgggg tgcgggtccg cctgctcgag cggtggcaaa ttgccgccgc ggcgtccggg
     2941 gccagtgccg gcggcgtccg gcagcagggc agagacctgc gggagttccc gctcgcattc
     3001 cgggcgctcg atcgctggcg aaccctcgaa gaagagctga acgccgacct cgactaccgt
     3061 cgcaatggcc atgccaccac ctgcgagcac gaggcggacc tggccgagct ggccaagtac
     3121 gtcctgatcc agcgatccgc tggtctcgac ctgcaactgg tcgagggcga cgagctccgc
     3181 atgttgatcc ccgggattgc gccgaccgtg atcgccgccg cctactcacc cgaagacggc
     3241 cacgccaatc cgacccggac cacgcaggcc ttcgccgacg ccgctaaacg gctgggcgcg
     3301 acgatccagt gcggcgtcga agcgaccggc ctggcgatgc aaggcgaccg cgtaatcggt
     3361 gtcgagacga cggatggccc gatttcaacc gggacggtcg tgctcgcagc cggggcctgg
     3421 agcggtgaga tcgccaagag catcggcctc aacctgcata tttcgccgga cggctatcag
     3481 gcgatgacca ccttcccggc cgatccgcac ctgacccagg tgctcggctc gatgcgacgg
     3541 ctgatcagtc tcaagcaact tccggacgga cgctacctgc tcggcggggg ctggcccggc
     3601 aacttctcac tggtcgcgcc gcgcggcgaa acgatcgagg agaacatcac ccggaatttc
     3661 gaggcgggtg cggcactgat cccacagctc ggggcggccg tgatcgacca cgcgtggatc
     3721 gggatggacg cccatacgca cgacgaggtt gcggtgctcg gcgaggctga cgggatcgac
     3781 gggctcgtgc tggcaaccgg cttctccggc ca
//
LOCUS       DASSGX010000564         2284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_864116, whole genome shotgun sequence.
ACCESSION   DASSGX010000564 DASSGX010000000
VERSION     DASSGX010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864116"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1195
                     /locus_tag="VFI12_10220"
     CDS             <1..1195
                     /locus_tag="VFI12_10220"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET7056828.1"
                     /translation="IFIDLDDFKRINDSLGHSAGDAVLIAVAARLAGCIRAGDTIARL
                     GGDEFTVLLDDCDLPEALAVVDRIHATLLEPLCIDGREMVISPSIGIALGEPEITLPE
                     EFLRRADVAMYVAKRTGKARHVLFSPEMDQHTLVSLDLEADLRRAIQRGELELHFQPI
                     MDTITGEVAALEALIRWNHPDRGQIGPGTFIPMAEESGLIVALGTWVVESACRQVAAW
                     QRERPGQPPIAVSVNISARQFQDQRLVELVRHALAETGISPHRLMIEITESVALSDLT
                     DTQATLNALRTLGVHLAIDDFGTGYSGLAYLQRCQIDTIKIDQSYIHGICVNPGDEAM
                     VRAVSAYASTLGVDVVAEGVETAAQAAKLREIGIVLGQGYLFAPPLSATDIETRYLTP
                     IRQTA"
     gene            complement(1319..1735)
                     /gene="tnpA"
                     /locus_tag="VFI12_10225"
     CDS             complement(1319..1735)
                     /gene="tnpA"
                     /locus_tag="VFI12_10225"
                     /inference="COORDINATES: protein motif:HMM:NF033573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HET7056829.1"
                     /translation="MSYWRLHYHLIWATKRRLPLIGSEEEAIVAESLRMTMSRMKIIG
                     HAIGMVEDHVHLALSIPPTVAVADAVGRLKGASSHLIGQHLEVTGFAWQSEYGALSLT
                     ERALPTVVDYVQNQKQHHVDNTLIHGLERIGQKQDQ"
     gene            complement(1842..>2284)
                     /locus_tag="VFI12_10230"
     CDS             complement(1842..>2284)
                     /locus_tag="VFI12_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056830.1"
                     /translation="NRPDATTESIRDGWYVTGDICVIDEDGFVRIVGRESRFAKIGGE
                     MVPHGLVEDALTAIVGVDEDGQQKVVVVSVPDPERGERLIVVHTPVEQRPAELRDALH
                     AAGLPNLFIPDGNSFLEVAELPTLGSGKLNIRQIKSVAETAFAI"
BASE COUNT          393 a          710 c          756 g          425 t
ORIGIN      
        1 catcttcatc gacctggatg acttcaagcg gatcaatgac tccctcggcc acagcgccgg
       61 cgacgccgtc ctgatcgcgg tggcggcgcg gttggccggc tgcatccggg caggcgacac
      121 gatcgcccgg ctcggcggcg atgagttcac cgttctgctc gacgattgcg atttgccgga
      181 ggcattggcc gtcgtcgacc ggatccatgc gaccctgctt gaaccgctgt gcatcgatgg
      241 acgggagatg gtgatcagcc ccagcatcgg cattgcgctc ggcgagccgg agatcacgct
      301 ccccgaagag tttctgcggc gagcggatgt cgccatgtac gtcgccaagc ggaccggcaa
      361 ggcccgccat gtcctcttca gcccggagat ggatcagcac acgttggtga gcctggacct
      421 ggaggctgat ttgcggcggg cgatccaacg gggcgagctc gagctccatt tccagccgat
      481 catggatacg atcacgggcg aggtcgcggc gctcgaggcg ctgattcgct ggaaccaccc
      541 ggatcgggga cagatcggcc ccggcacgtt cattccgatg gccgaggaga gcgggttgat
      601 cgtcgcgctg gggacgtggg tggtggagtc ggcctgccgg caggtcgcgg cctggcagcg
      661 ggagcggccg ggccagccgc cgatcgcggt cagcgtcaac atctcggccc ggcagttcca
      721 ggatcagcgg ctggtcgagt tggtccggca cgcgctggcg gagaccggga ttagcccgca
      781 ccggttgatg atcgagatca ccgagtcggt cgcgctcagc gatctgaccg atacgcaggc
      841 gaccttgaat gcactgcgga cgctgggcgt gcatctggcg atcgacgatt tcgggaccgg
      901 ctattcgggg ctggcctact tgcagcgctg ccagatcgac acgatcaaga tcgaccagtc
      961 ctacatccac ggaatttgcg tgaatccggg cgacgaggcg atggtccgag cggtctcggc
     1021 ctatgccagc acgctcgggg tcgatgtcgt ggccgagggg gtcgagacgg cggcgcaggc
     1081 ggcgaagctg cgggaaatcg gcatcgttct gggccagggg tatcttttcg ccccaccgct
     1141 ctccgccacc gacatcgaaa cccggtatct gaccccgata cggcaaacgg catagtcggc
     1201 gcctgacata ggggcccgtc gttccattcc cgcggcctga ccaccccggc ctaaagaccg
     1261 gggctgaaat ctgaacccgg ctgaagccgg ctggggaggt ctcggttcag ggacctggct
     1321 actgatcctg cttttgcccg atgcgttcga gcccatggat caacgtgttg tcgacgtggt
     1381 gctgcttctg gttttgcaca tagtcaacga ccgtgggcaa cgcccgctcc gtcagactga
     1441 gcgccccgta ttccgattgc caagcgaatc ctgttacctc gagatgttga ccaatcaaat
     1501 gggaggacgc ccctttgaga cgaccgacgg catcagcaac cgcgaccgtc ggagggattg
     1561 agagcgcaag gtggacatgg tcttccacca tgccgatcgc gtggccgatt atcttcatgc
     1621 gagacatcgt catccgcaat gactcggcaa cgattgcctc ttcttcggag ccgatcaggg
     1681 gcaaccgccg tttggtcgcc cagatgaggt ggtaatgcaa gcgccagtag gacatgccgg
     1741 tattatctgc tggtgtcctg acaaatgctt gcttcctacc cggcttcagc cgggttcagc
     1801 tttcagcccg ggtcttcagg cccgggcata tcggcgcggc gtcagatggc gaacgctgtc
     1861 tcggccacgg atttgatttg acggatgttg agtttgccgg agccgagggt ggggagttcg
     1921 gcgacctcga ggaagctgtt gccgtcgggg atgaagaggt tgggaaggcc ggcggcgtgc
     1981 aatgcgtccc gcagctcggc cgggcgctgc tctacgggtg tatggacgac gatgagccgc
     2041 tcgccacgct ccggatcagg cacggagacg acgacgacct tctgctggcc gtcctcgtca
     2101 acgccgacga ttgcggtcag cgcgtcctcg accagcccgt gcggcaccat ctcgccgccg
     2161 atcttggcga accggctctc ccgcccgacg atccgcacga aaccgtcctc gtcgatcacg
     2221 caaatgtcgc cggtcacgta ccagccatcc cggatcgatt ccgtcgtcgc atccggccgg
     2281 ttga
//
LOCUS       DASSGX010000565         2230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_534159, whole genome shotgun sequence.
ACCESSION   DASSGX010000565 DASSGX010000000
VERSION     DASSGX010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534159"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VFI12_10235"
     CDS             <1..204
                     /locus_tag="VFI12_10235"
                     /inference="COORDINATES: protein motif:HMM:NF025386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HET7056831.1"
                     /translation="IGPRGFAKTFGPRGVRTTVGIPGTGLYYTKLQARGNRPSVRRRC
                     PHCSRVVALSASFCPYCGTRLGS"
     gene            357..572
                     /locus_tag="VFI12_10240"
     CDS             357..572
                     /locus_tag="VFI12_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056832.1"
                     /translation="MDIERGCRSKQAYLTKADAKRIARSMSARHREAFHLYRCPNCRY
                     FHVGHLVPAAFRARVVPNWSRTAVQLA"
     gene            948..1220
                     /locus_tag="VFI12_10245"
     CDS             948..1220
                     /locus_tag="VFI12_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056833.1"
                     /translation="MLRWSINNEAFIKAVQAISFPAEFVGDQHALVRANTNLDALIRN
                     AYGGATSLSNLGYYWNPKLDKAELSAVQASALVRADLGLPPVPTHL"
     gene            1243..1626
                     /locus_tag="VFI12_10250"
     CDS             1243..1626
                     /locus_tag="VFI12_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056834.1"
                     /translation="MLIDIDSGREITAIPFEHDFEVLRGRLTTREFEAMVDEIDRLIE
                     ASGGEVATAGWLPGSDWTGTPFQPIYEKAAPADFNRAAMFFGQLVWYAVMHRPERWGS
                     GRYKVGDHDIGSRTYFRLANAGERS"
     gene            complement(1623..>2230)
                     /locus_tag="VFI12_10255"
     CDS             complement(1623..>2230)
                     /locus_tag="VFI12_10255"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HET7056835.1"
                     /translation="TSELAREPDLLDRVAEAMRSQGIDARIVETGAEARELVLELVPV
                     GAEVHSGKSKTIEDLGLFAELMESGKYDAVRPKLRSMDRDTQGREMRKLGAAPDFELG
                     SVAAVTEDGALITASATGNNLAAYAGGAGKVIFVVGSQKIVKDLPAALDRIRDTVFPY
                     EDGRVLETMGVHTKLAKVLITYGEWIKDRTTVILVREPVGV"
BASE COUNT          367 a          745 c          736 g          382 t
ORIGIN      
        1 atcggcccgc gcggcttcgc gaagacgttc ggcccgcgcg gcgtgcggac aacggtcggg
       61 atcccgggca ccgggctgta ctacacgaag ctccaggcgc gcggcaatcg accctcggtg
      121 cggcgtcgat gtccccattg cagccgggtc gtcgccctct cggcgagctt ctgcccgtac
      181 tgcgggactc ggctggggag ctaggcgcgg gcgtacggcc cgcgctccgg agcttgtgac
      241 actcaaggtg acacggggag cgcgcacgtt cgcgccgaca tcgagacgag ccacgatcgg
      301 ccagctctga cgaccgatcg gcgtaccggg ccggaggcga tgtcggaagg gcaccgatgg
      361 acatcgagcg tggctgtcga tccaagcagg cctacctgac caaggcagac gcgaagcgca
      421 tcgcgcgcag catgagcgcg cgccaccgcg aggccttcca cctgtaccga tgcccgaact
      481 gccggtactt ccacgtcggg cacctggtgc cggcggcgtt ccgggcccgg gtggttccga
      541 attggtcgcg gactgccgtg cagctcgcct agggcgtctc gatcgggagg aaggtaggca
      601 tgggccatcc ccttgacgct gggcgaaagg cggtcaagat ccggccgagg gaggcctgtg
      661 tgagtcgcaa caagtggctt ctgctggttg ggctattggt tggcgcgtgt tccgggtctc
      721 cgtcgtccgc gcccggatct gaagcggtct cccaatggac gcctacagca accgtcccga
      781 ccgctccgcc aagcccgacg cctgtgccca gtccttcgcc aacaccggat ctaacagcct
      841 cagccgcagc ggcatacctg gccgctgtaa cggcgtacaa ctccgctcaa aaggtcatcc
      901 agaagacccc ctgccaagta tccacgctca aggcgcagaa gagttgcttg ctgagatggt
      961 ccatcaataa cgaggcgttc attaaggccg ttcaggcgat ctcgtttcct gcggagttcg
     1021 tcggcgatca gcacgccctc gtccgggcga acacgaacct cgacgccctg atccggaacg
     1081 cgtacggggg cgcaacctcg ctctcgaatc ttgggtacta ctggaatccc aagctggaca
     1141 aggcggaact ctcggcggtc caggcctcgg cgctcgtccg agctgatctt ggtctgccgc
     1201 cagtgccgac gcacctgtga caacctttag cgttctcccg acgtgctaat agacatcgac
     1261 tccgggcgcg aaatcacggc aattccgttc gagcatgact tcgaggttct gcggggccgc
     1321 ctcacgaccc gtgagtttga ggcgatggtc gacgagattg atcgcctcat cgaagcaagc
     1381 gggggagagg tcgccacggc cggttggctg cccggcagcg actggacagg aaccccgttt
     1441 cagccgatct acgagaaggc ggccccagcg gacttcaacc gtgccgcgat gtttttcggg
     1501 cagctcgtct ggtatgcggt gatgcatcga ccagagcgtt ggggctccgg acggtacaag
     1561 gtcggcgatc acgacatcgg tagccggact tacttccgac tcgccaacgc gggcgaacgt
     1621 tcctagaccc cgaccggctc ccgcaccagg atcaccgtcg tgcggtcctt gatccactcg
     1681 ccgtaggtga tgaggacctt ggcgagcttc gtgtggacgc ccatcgtctc caggacgcgg
     1741 ccgtcctcgt acgggaagac cgtgtcgcgg atccgatcga gcgcggcggg gaggtctttg
     1801 acgatcttct ggctgccgac gacgaagatg accttgcccg cgccgcctgc gtaggccgcg
     1861 aggttgttgc cggtcgccga ggccgtgatg agcgcgccgt cctcggtgac cgccgcgacg
     1921 ctgccgagct cgaagtccgg cgccgccccg agcttgcgca tctcgcggcc ctgcgtgtcc
     1981 cgatccatcg agcggagctt cggccgaacc gcgtcgtact tgcccgactc catgagctcg
     2041 gcgaacagcc ccaggtcctc gatggtcttg ctcttgccgg agtggacctc ggcccccacg
     2101 ggcacgagct cgaggaccag ctcgcgcgcc tcggcgccgg tctcgacgat ccgggcatcg
     2161 atgccctggc tgcgcatcgc ctcggcgacg cgatcgagga ggtcgggctc gcgtgccagc
     2221 tcggaggtga
//
LOCUS       DASSGX010000566         2706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1625891, whole genome shotgun sequence.
ACCESSION   DASSGX010000566 DASSGX010000000
VERSION     DASSGX010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1625891"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1187)
                     /locus_tag="VFI12_10260"
     CDS             complement(<1..1187)
                     /locus_tag="VFI12_10260"
                     /inference="COORDINATES: protein motif:HMM:NF006769.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HET7056836.1"
                     /translation="MQQPLSINVDRLHASLAELARIGATPAGGVTRLALSDEDRQARD
                     LLCTWLRDAGLDPNVDDVGNISAIRPGRDAGPPVLLGSHIDTVVRGGKFDGALGVMGA
                     LEVIRTLNDNGIETRLPVGLVSWTNEEGVRFEPAMTCSGVVTGRFTPEYVGERMDRAG
                     LRFADELARIGYQGDAANRPLPATAYLELHIEQGPVLEARGLPAGVVGGIVGITWNEV
                     TVSGQTDHAGPSPMHLRHDALTAAAEIILGVEQIAKQRDETAVATVGRIAAQPNVINT
                     IPGEVIFSVDFRHSDPAILEGQVQRLHELVDHVASRRGVEVAIERFWTSEPTPFDPNV
                     QAAIRDAATAFDIAVGELWSGAGHDAKYLADVCPSGMIFVRREGGLSHCETEYSTTAD
                     IEAG"
     gene            complement(1254..2099)
                     /locus_tag="VFI12_10265"
     CDS             complement(1254..2099)
                     /locus_tag="VFI12_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056837.1"
                     /translation="MPLPQPEPEPEPKKDKKKRKSKEPAPVGTVPAAPPDSYSYSYGS
                     PPSTAATSMAVVDAPDGSGKNKSGGRLRLLLVLLLLLAGGVSGMMYWNEHPSSASGRV
                     NWDALTEFDFANVWNPTDDATEAGTDSDLMAQIPEGAVQARAISVAEDGLITIRYKGK
                     NTDVRLAGVPADFADQCLGAKGVARLDRILPKGAVIYVLLDGEERLGATEADGPHPQF
                     VYLWRVDADANKVRYANQELVVSGEAELAPVTRFDHPAAQQLEQASMRATEKQKGRFG
                     SGACP"
     gene            2430..2585
                     /locus_tag="VFI12_10270"
     CDS             2430..2585
                     /locus_tag="VFI12_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056838.1"
                     /translation="MQHALSTLFLVWGLMIVVTIWIAANALADFGARRLRHWYHSARH
                     HTHAGDE"
BASE COUNT          411 a          872 c          942 g          481 t
ORIGIN      
        1 ccggcttcga tatcggccgt cgtgctgtat tcggtttcgc agtggctgag cccgccttct
       61 ctgcggacga agatcatccc gctcgggcac acatcggcca ggtacttggc gtcgtgcccg
      121 gcgccggacc agagctcgcc aaccgcgatg tcgaacgcgg tcgcggcgtc ccggatcgcc
      181 gcctggacat tcggatcgaa cggtgtcggc tcgctcgtcc agaagcgctc gattgcgacc
      241 tcgacgccgc gccgggacgc gacatggtcg accagctcgt gcagcctctg gacctgccct
      301 tcgaggatgg ccgggtcgga gtggcggaaa tcgacgctga agatgacctc gccggggatc
      361 gtgttgatga cgttcggctg ggcggcgatc cggccgacgg tggcgacggc ggtctcgtcc
      421 cgctgtttcg cgatctgttc gacgccgagg atgatctcag ccgcggcagt gagagcgtcg
      481 tggcgaaggt gcatcgggct cgggccggca tggtcggtct ggccgctgac cgtgacctcg
      541 ttccaggtga tgccgacgat gccaccgacg accccggccg ggagtccccg cgcctcgagg
      601 accggaccct gctcgatatg cagctcgagg taggcggtgg ccgggagcgg gcgattggcg
      661 gcatcgccct ggtaaccaat ccgggccagc tcatcggcga agcggaggcc ggcgcggtcc
      721 atccgctcgc cgacgtactc gggcgtaaag cggccggtga ccacgccgga gcaggtcatg
      781 gccggctcga agcggacgcc ttcctcgttg gtccagctga ccaggccgac cgggagccgg
      841 gtctcgatgc cgttgtcgtt gagggtgcgg atgacctcga gcgcgcccat gacgccgagc
      901 gcgccgtcga acttgcctcc gcgcacgacg gtgtcgatgt gggagccgag caggaccggt
      961 gggccggcgt cgcggccggg ccggatcgcc gagatgttac cgacgtcgtc gacgttcggg
     1021 tcgagtccgg cgtcccgcag ccaggtgcaa aggagatcgc gggcctggcg atcttcatcg
     1081 gagagggcga ggcgcgtgac gccgccggcc ggggtcgcgc cgatgcgcgc gagctcagcc
     1141 aggctggcgt gcagccgatc cacgttgatt gacagaggtt gctgcacgtc gattccgcct
     1201 ccccggatgg aacgccatcc aattgggcgg aatcatacgg gcaggatcgc ggatcaggga
     1261 caggcgccgg acccgaaccg gcccttctgc ttctcggtcg cgcgcatcga tgcctgttcg
     1321 agctgctggg cggcgggatg atcgaagcgg gtcaccggcg cgagctccgc ctcaccgctg
     1381 acgaccaact cctggttcgc gtatcggacc ttgttcgcgt ccgcgtcgac ccgccacagg
     1441 tagacgaact ggggatgcgg gccatccgcc tcggtcgcgc caagacgctc ctcgccgtcg
     1501 agcaggacgt agatcaccgc tcctttcggc aggatgcgat caagccgggc gacgcccttg
     1561 gcgccgaggc actgatccgc aaagtcggcc gggactccgg ccaggcggac atccgtgttc
     1621 ttgcccttgt atcggatcgt gatcaggccg tcttcggcga cggagatggc ccgggcttgg
     1681 acggcgccct ccggaatctg ggccatgagg tcactgtctg tcccggcttc ggtcgcgtca
     1741 tcggtcgggt tccagacgtt ggcgaaatcg aactcagtca gcgcatccca gttgacccgg
     1801 cccgaggcgc tggagggatg ctcgttccag tacatcatcc cgctgacacc gccggcgagg
     1861 agcagaagca gcacgaggag gagacgcagg cgcccgccgg atttgttctt gccactgccg
     1921 tccggcgcat cgaccacagc catggacgtc gccgcggtgg atggcggcga gccatatgag
     1981 tacgaatagc tatcgggcgg agcggccggg acagttccga ccggcgccgg ctccttcgac
     2041 ttgcgcttct tcttgtcctt tttcggctcg ggttccggtt ctggttgggg tagcggcatc
     2101 tccgcttctg atctcagttc cgcgccgcac gcgagacaga accggtagtt cgggctcgct
     2161 tccgcgttgc aatgaggaca ttgatttcgc gattgagcca ttggcgctcc tcacgaccta
     2221 aaccggacac cgactgatac gggcatgccc cgcgccggtt ccatgttcgg ggggaatggc
     2281 gctcggccct atcccccgga ggtgccaaga attcgggggt atttccgctc ggtacaggcg
     2341 gggcacgcgg cgcatgttga caacggaaag gggtgtacgt acagttcggt cagtcgcggt
     2401 gggacaatcg cattggggga tgttcaggca tgcagcacgc cctctcgacg cttttcctgg
     2461 tttggggctt gatgatcgtc gtcacgatct ggatcgctgc caacgccctg gccgacttcg
     2521 gcgcccgcag acttcgccac tggtatcact cggcccggca ccacacccac gccggcgacg
     2581 aatagcgacg ttagcggtcg tccagactgc tttcacgcgc tcggagggtc agccccggca
     2641 gtggcggggc tgagcgtcgt catgcagtcg gcgcggccgg ggtcagccca tggcggcgca
     2701 tgatgt
//
LOCUS       DASSGX010000567         3845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_316117, whole genome shotgun sequence.
ACCESSION   DASSGX010000567 DASSGX010000000
VERSION     DASSGX010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316117"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /gene="rimI"
                     /locus_tag="VFI12_10275"
     CDS             <1..748
                     /gene="rimI"
                     /locus_tag="VFI12_10275"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HET7056839.1"
                     /translation="RSGITPQWTGTGPTMIQAQPDVALSIEHMQIADIDEVSRIERRS
                     FSNPWPMSAYRRELRRPEQNSYYVLRARPVPQHDAGELRGTRGRFLETILPVRRGDPP
                     RDEPHLAAYVGMWQMYDETHITTIAVDPVYRGRGYGELMLVTAFADAMARRSAWVSLE
                     VRVTNDPAQALYRKYGMSVYGRRAAYYTDNHEDALVMWSRSLRDREYGDQISDLRDRL
                     VLRLPEIDVPEFLAPGTFDEFDSSPESPIP"
     gene            745..1836
                     /gene="tsaD"
                     /locus_tag="VFI12_10280"
     CDS             745..1836
                     /gene="tsaD"
                     /locus_tag="VFI12_10280"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HET7056840.1"
                     /translation="MNILAIETSCDETAAALIADGRHVRSNVVWSQLAAHQRYGGVVP
                     ELAARAHLTAIGPVVRDALSGADATWDDVDVVAVTNRPGLSGALLVGVNAAKGIAFAR
                     DLPLVGVHHIEGHIAANWLIDELQNYGEIAMPAICLIVSGGHTELVLMREPGAYELLG
                     TTLDDASGEAFDKGARLLGLGYPGGPAIQHAAETGDPDAFPLPRAWLNDTFDFSFSGL
                     KTALLRLVEPYRTEDASAPAPVKEPFAAHVPPAFRPDMPVADLAAGFQEAIVDVLVGK
                     TVAAARDAGAKTVILAGGVAANARLRSAMEAAVAGLEPPVRLIIPRFAYCTDNAAMIG
                     AAAHWMIQRGELSGYDLDVVAREPLAKKD"
     gene            1846..2670
                     /locus_tag="VFI12_10285"
     CDS             1846..2670
                     /locus_tag="VFI12_10285"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET7056841.1"
                     /translation="MSSHDETTAVFETAIRLAYDAGRLLRDRVGRQIEVSYKGAIDLV
                     TEVDRAAEALIGQGIADAHPSHAMLGEEGTSTGVAVAAAEWVWVVDPIDGTTNFAHGL
                     PHFAVSIAVVRQGVGEIGVIYDPSRDELFSARRGSRATLNGRPIQVSTVDAIGRSLLG
                     TGFSYDTASQVEQHAIWRHLHSYCQGIRREGSAALGVAWLAAGRTDGFYERPINAWDV
                     GAGAIIVEAAGGRVTAIDGGPYDVFGQEILASNGLIHTDLVNAINAATSDVGSPVT"
     gene            2674..3129
                     /locus_tag="VFI12_10290"
     CDS             2674..3129
                     /locus_tag="VFI12_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056842.1"
                     /translation="MDDPDNHDAPEDFEERLQELLGTADVMSILFTRVAHSLIIDYRQ
                     TETVGPRVMTDEIVASAHDRFLSFGRMRPHLPLPDQLTLAFWTPGVREFQDSGMLQIM
                     VERCMASGGQALVDEAQDSYRFLLKLERQYLCDMVRGVGMKTLWERPRE"
     gene            3172..3396
                     /locus_tag="VFI12_10295"
     CDS             3172..3396
                     /locus_tag="VFI12_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056843.1"
                     /translation="MRAYVLITIEAGKGSEIQKALLDAGIGQVDQLAGIYDLVAVIDA
                     QDPRRIGEIVIGTIQKTPGVVSTVTLLTIG"
     gene            3510..>3845
                     /locus_tag="VFI12_10300"
     CDS             3510..>3845
                     /locus_tag="VFI12_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056844.1"
                     /translation="MRDERKRYTTQLQRRAQAHITAELGGTTVVPDTDRQVTMVIQHA
                     QAATGARRVSFFRPVTRGRRWHVATVLEDGAFYYGLSCPDTMGWSRISYEQRRPVLFD
                     VDDRRPETGP"
BASE COUNT          670 a         1182 c         1303 g          690 t
ORIGIN      
        1 tcggagcgga ataaccccgc agtggaccgg gaccggacca accatgattc aggcacaacc
       61 agacgttgcc ctctctatcg agcacatgca aatcgcggat attgacgagg tatcccggat
      121 cgagcgcaga tccttttcca atccctggcc gatgtctgcc taccgccggg agctgcgccg
      181 gcccgagcag aactcctatt acgtgcttcg tgcgcgtcct gtgccgcagc acgatgccgg
      241 cgagctccgg ggaacgcgcg ggcgtttcct ggaaacgatc ctgccggtcc gccgcggcga
      301 tccgccccgg gacgaaccgc acctcgccgc ctatgttggt atgtggcaga tgtacgacga
      361 aacccacatc acgacgattg cggtcgaccc ggtctatcgc ggccgggggt atggcgagct
      421 gatgctggtc accgcctttg ccgacgcgat ggccaggcgc tcagcctggg tttcgctcga
      481 ggtgcgagtc accaacgatc cggcgcaagc gctttaccgg aagtacggga tgagcgtata
      541 cggtcgccgt gccgcctatt acacggacaa ccacgaggat gcccttgtga tgtggtcgcg
      601 gtcgctgcgg gaccgggagt acggcgacca gatcagtgat ctgcgtgatc ggctcgtgct
      661 ccgcctgccc gagatcgatg tccccgagtt ccttgctccc gggacattcg atgagtttga
      721 ctccagtcca gagtcgccaa ttccgtgaac atcctcgcaa tcgaaacctc atgtgacgag
      781 actgcggcgg ccctgatcgc cgacggtcgg cacgtacgct cgaatgtcgt ctggtcacag
      841 ctcgccgcgc atcagcggta cggcggtgtc gtgccggagc tggccgcgcg ggcacatctg
      901 acggcgatcg gtccggtcgt gcgcgatgcc ctcagcggcg ccgatgcaac ctgggacgat
      961 gtcgatgtgg tcgccgtcac gaaccggccg gggttgagcg gagcactgtt ggttggcgtc
     1021 aacgccgcca aggggattgc ttttgcgcga gacctgccgc tggtcggggt ccatcacatc
     1081 gagggacaca tcgccgccaa ctggctgatc gacgaactgc agaactatgg cgagatcgcg
     1141 atgccggcga tctgtctgat cgtctccggc ggccacacgg agctcgtgct gatgcgggag
     1201 ccgggcgcgt acgaattgct cggcacgacg ctcgacgatg cgtccgggga ggcgttcgac
     1261 aagggggccc gcctgctcgg actcgggtat cccggcggcc cggcgattca acacgcggcg
     1321 gaaaccggcg atccggatgc gtttccgctt ccgcgcgcct ggctgaacga cacgttcgat
     1381 ttcagcttct cgggtctgaa gacggcgctc cttcgtctcg tcgagccgta tcggaccgag
     1441 gacgcgtcgg ctcccgcacc ggtcaaggag ccattcgcgg cccatgtgcc gccagcgttt
     1501 cgccccgata tgccggtcgc ggatctcgca gccggattcc aggaggcgat cgtagacgta
     1561 ctggtcggga agaccgtggc cgccgcccgg gatgcaggcg cgaaaacagt gatcctggca
     1621 ggcggcgtag ccgcgaacgc ccggctacgt tcggcgatgg aggcggcagt cgcggggctg
     1681 gaaccgccgg tgcggctgat catcccccgg ttcgcctatt gcaccgataa tgccgcgatg
     1741 atcggtgcgg ctgcgcactg gatgatccag cggggtgagc tttccgggta tgacctcgac
     1801 gtcgtcgccc gggagccatt ggcaaagaag gattaggttc aggaattgag tagtcacgac
     1861 gagacaacgg cggtatttga gacagcgatt cggctggcgt acgacgccgg ccgcctgctg
     1921 cgggatcgcg tggggcgaca gatcgaggtc tcgtacaagg gcgcgatcga ccttgtcacg
     1981 gaggtggacc gcgccgccga ggcgttgatc ggccagggaa ttgcggatgc acacccgtca
     2041 cacgcgatgc tcggcgaaga gggcaccagc accggggttg cggtcgccgc ggcggagtgg
     2101 gtctgggtcg tcgacccgat cgacggcacc acgaacttcg ctcatggcct tccgcatttt
     2161 gccgtctcga tcgccgtcgt ccggcagggc gtcggggaaa tcggcgtcat ctacgatccc
     2221 tcgcgcgacg aactgttctc ggcgcgccgg ggaagccggg cgacactcaa cggccggccc
     2281 attcaggttt cgacggtcga tgcgattggt cggtcgctgc tcggtacggg attctcgtat
     2341 gacaccgcca gccaggttga acagcacgcg atctggcggc atctacacag ctattgccag
     2401 ggcattcgac gggagggttc ggcggcgctg ggcgtggcct ggctcgctgc cggccgcacg
     2461 gacggctttt acgagcgtcc gatcaatgcc tgggatgtcg gcgctggcgc gatcatcgtc
     2521 gaggcggccg gcggccgggt gaccgccatt gacggtggtc cctacgatgt ttttggacag
     2581 gagattctgg ccagcaacgg gctgattcac accgatctgg taaatgcgat caatgccgcg
     2641 acgagcgatg ttggttcgcc ggtgacatag accatggatg atccggataa ccacgacgcg
     2701 ccggaagact tcgaggagcg tctccaggaa ctgctcggca cggccgatgt gatgagtatt
     2761 ctcttcacgc gcgtcgcgca ctcgctgatc atcgattacc ggcagacgga gacggttggc
     2821 ccacgggtaa tgacggacga gatcgtggcg tccgcgcacg atcgcttcct gtcattcggg
     2881 cggatgcgcc cgcatctgcc attgcccgat caactcacac tcgccttttg gacgccgggg
     2941 gtccgcgaat ttcaggatag cggcatgttg cagatcatgg tcgagcggtg catggcgagt
     3001 ggcgggcagg ccctggtgga cgaggcgcag gacagctatc gcttcctctt gaagttggaa
     3061 cgacagtacc tttgcgatat ggtgcgcggt gtcggcatga agacattatg ggagcgtccc
     3121 cgggagtaac tcccgatttc ggggttggtc cggggccagg gatgtaacgc ggtgcgcgca
     3181 tacgtgctga tcacgatcga ggcgggcaag gggtcggaga tccagaaagc gctgctggac
     3241 gcgggaatcg ggcaggtgga ccaacttgcc ggaatctacg atctggtagc agtgatcgac
     3301 gcccaggacc cgcgcaggat cggtgagatc gtgatcggca cgattcagaa gacgcccggc
     3361 gtggtgagca cggtaacgct cctcacgatc ggctgattcc agcgcgcacc ggcaagcgac
     3421 gaaagtgacc attgcgcatg ccgccaattg actccgcccc gctgccgctc gttggagccg
     3481 aggagccgtt ttccttttcg gaagaaccca tgcgcgatga gcgcaagcgg tacaccaccc
     3541 agctccaacg ccgcgcccag gcccacatca cggccgaact gggcggcacg acggtcgtgc
     3601 cggatacgga tcgccaggtg acgatggtca tccagcacgc ccaggcggcg accggcgccc
     3661 gccgggtctc gttctttcgc ccggtgacgc gcggtcggcg atggcacgtc gcaaccgtgc
     3721 tcgaggacgg cgccttctat tacgggttgt cctgccccga cacaatgggc tggtcccgga
     3781 tctcgtatga gcagcggcgg ccggtattgt tcgacgtgga cgatcggcga ccggaaaccg
     3841 ggccg
//
LOCUS       DASSGX010000568         2430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_752939, whole genome shotgun sequence.
ACCESSION   DASSGX010000568 DASSGX010000000
VERSION     DASSGX010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_752939"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VFI12_10305"
     CDS             complement(<1..412)
                     /locus_tag="VFI12_10305"
                     /inference="COORDINATES: protein motif:HMM:NF024740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056845.1"
                     /translation="MTPEHESVQRLRNARGWIFDMDGVLYRGTEGLPGVRALFDELDH
                     RGLPYRLATNNSMASPLMYVDRLAGMGIRADKSSILTSAMATRQYLLDHIGQKAKLHV
                     LGMPALTDQLLAHGDFSIVDPDVETPDAVVVGLDR"
     gene            complement(434..1342)
                     /locus_tag="VFI12_10310"
     CDS             complement(434..1342)
                     /locus_tag="VFI12_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056846.1"
                     /translation="MTSAEALLIDLGGSDPLITELIAETGPLLVPFDTLTDVDGWVVD
                     LNGTGSNVPFDAPDLASERRAVNALLVATDYAIRRGATNVADVIRVLNRESPVSVSGT
                     ELLSDIVRSVVVRAGIDGRTPAAFKRRRRGHQLTAGSPSDPAISFKPRSMDKDIGDNS
                     HSSLLILAEGASDELGVEGEIGERWGVEIGLPAEVTLDTIAEIEREVAIMPSYIRGLT
                     SYADVDGIVLGWPDGQEPDPHTVAVVIQGWTKALFDLHAADVRIVFAPATGESAVLTD
                     MRARATAARLYRDAMRRQGSTATEPE"
     gene            complement(1339..1869)
                     /locus_tag="VFI12_10315"
     CDS             complement(1339..1869)
                     /locus_tag="VFI12_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HET7056847.1"
                     /translation="MAKNIAMVLANNFEDIEATSPKEYLESQGANVTVIGLDSNTIHG
                     KKGATIEPDTTFDQLGKDGIDRFDALVIPGGGSPENLRIDRGAVDFTKRFFDTGKVVA
                     SICHGPQLLISAGVLNNRTTTSVNKIRDDFSNAGAHYVDEPVAVDGNLISSRTPADLD
                     MFNHAVAYALGLTSEE"
     gene            complement(1870..>2430)
                     /locus_tag="VFI12_10320"
     CDS             complement(1870..>2430)
                     /locus_tag="VFI12_10320"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF015113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HET7056848.1"
                     /translation="GAFYGPKIDIKWVDALGREWTGPTVQLDFNLPQRFDITYIGEDG
                     ERHRAVMIHRTLLGSMERFVGGLVEHYAGAFPVWLAPTQIALIPITDDQVAYCQEIMD
                     KAQAMELRVELFDQKERMQAKIRDAQLMKIPYMLVVGKREVENGAVAVRLRSGEDLGA
                     MPVDDFLAMAKEIVAAKSLEQTVKEA"
BASE COUNT          429 a          794 c          745 g          462 t
ORIGIN      
        1 ctcggtccag gccgacaacc accgcgtccg gcgtctcgac atcgggatcg acgatcgaga
       61 agtcgccgtg ggccagcaat tggtcggtca gggccggcat tccgagcacg tggagcttcg
      121 ccttttggcc gatatggtcc agcaggtatt gccgcgtcgc catcgccgac gtcagaatcg
      181 aggatttgtc ggcacgaatt cccatgccgg ccaaccgatc gacatacatc agcggcgacg
      241 ccattgaatt gttcgtcgcc agccggtacg gcaagccgcg gtggtccagc tcgtcgaaca
      301 gcgcccgcac gcctggcagc ccttctgtgc ctcgatacag gacgccgtcc atatcgaaga
      361 tccagccccg cgcgttgcgc agccgctgga ccgattcatg ctcaggggtc attcaccctc
      421 cccggggaaa cgatcattcg ggttcagtcg ccgtcgaacc ctgccggcgc attgcgtcac
      481 gatacaaccg ggcggccgtt gctcgcgccc gcatatcggt caggacggcc gattcaccgg
      541 tcgcgggcgc gaaaacgatc cgcacgtcgg cggcatgcag atcgaagagc gccttggtcc
      601 agccctggat gacgacggcg acagtgtgcg ggtccggctc ctgtccatcc ggccagccga
      661 gcacgatccc gtcgacatcc gcatagctgg tcaggccccg gatatacgac ggcatgatcg
      721 cgacctcgcg ctcgatctcg gcgatggtgt cgagcgtcac ttcagcaggg agaccgattt
      781 cgacacccca tcgttcgccg atttccccct ccaccccgag ctcatcggac gcaccctcgg
      841 caagaatcag gagcgacgaa tgcgagttgt cgccgatgtc cttgtccatg gagcggggct
      901 tgaaggagat ggcgggatcc gatgggctcc cggcggtcaa ctggtgccct cgccgccgtc
      961 gcttgaacgc cgcgggagtg cgcccatcaa ttccggcccg caccacgacc gatcgaacga
     1021 tgtccgaaag cagctcggtt ccggacactg aaaccggtga ttcccggttc agcacccgga
     1081 ttacatcggc gacgttcgtt gcgccgcgcc ggatcgcata gtcggtggca acgaggagcg
     1141 cattgacggc gcgtcgctcg gacgcgaggt ccggggcgtc gaatgggacg ttggagccgg
     1201 tgccgttaag gtcgacgacc cagccgtcga cgtcggtcaa cgtgtcgaat ggcacgagga
     1261 gcggcccggt ctccgcgatc aactcggtga tcaacggatc cgagccgccc aggtcaatga
     1321 ggagcgcctc tgcggatgtc actcttcgga tgtcagcccg agtgcgtagg caaccgcgtg
     1381 gttgaacatg tcgaggtcgg ccggcgtgcg cgacgagatc aggttcccgt cgacggcgac
     1441 tggctcatcg acatagtgag cgccggcatt cgagaagtcg tcgcgaatct tgtttaccga
     1501 agtcgtcgtc cggttgttga ggacaccagc cgagatcagt agttgcggcc cgtgacagat
     1561 cgacgccacg accttgccgg tgtcgaagaa gcgcttcgtg aagtcgaccg ccccgcggtc
     1621 gatgcgcagg ttctcgggcg aaccgccgcc cgggatgacg agcgcatcga accggtcaat
     1681 gccgtccttg ccgagctggt caaaggtcgt gtccggctcg atcgtcgcgc ctttcttgcc
     1741 gtggatcgtg ttcgagtcga gcccgatcac cgtcacgttc gccccctggc tctcgagata
     1801 ttccttcggc gaggttgcct cgatatcctc gaagttattt gccagcacca tcgctatgtt
     1861 ctttgccatt tatgcctcct ttacggtctg ctccaggctc tttgctgcca cgatctcctt
     1921 cgccatcgcg aggaaatcgt cgacaggcat tgcgcccagg tcctcgcctg atcgtagccg
     1981 aaccgcgacc gcgccgttct cgacctcgcg cttcccgact accagcatgt aggggatctt
     2041 catgagctgg gcgtcgcgga tcttggcctg catgcgctcc ttctggtcaa agagctcgac
     2101 ccgcaattcc atcgcctgcg ccttgtccat gatttcctgg cagtaagcga cctgatcatc
     2161 cgtgatcggg atcagggcaa tctgcgtagg cgccagccag accgggaagg cgccggcata
     2221 atgctcgacg agcccgccga cgaaccgctc catcgatcca agcaaggttc gatggatcat
     2281 cacggcgcga tggcgctcgc catcctcacc gatataggtg atgtcgaagc gctgcggcag
     2341 gttgaagtcg agttggaccg tcgggcccgt ccactcccgg ccgagcgcgt cgacccactt
     2401 gatatcgatc ttcggcccgt agaaggcgcc
//
LOCUS       DASSGX010000569         4704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_427251, whole genome shotgun sequence.
ACCESSION   DASSGX010000569 DASSGX010000000
VERSION     DASSGX010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4704
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427251"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VFI12_10325"
     CDS             <1..334
                     /locus_tag="VFI12_10325"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET7056849.1"
                     /translation="VEALSTYGRLRRGYIGVGAQAVGLSASQAQAAKLDREVGLVILS
                     LDEDGPAQKAGLLIGDILVKVNGSPVAAVDDLVDHLSGDLIGSTIPLVVVRGAGTEDL
                     SVTVGERD"
     gene            327..998
                     /locus_tag="VFI12_10330"
     CDS             327..998
                     /locus_tag="VFI12_10330"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET7056850.1"
                     /translation="MISTTERTGTRRTDIRSAGTLARPAASPRVAVFASSPALRAGLI
                     TLLTEDDRVDVLSDDALARGEAPDVILLESGAGDLLDELIDDNWPRSALLFVGEPPDE
                     ALASGDRVTGAVSAGIEPARLSAAVQAMAAGLAVLDPDLEAALPLHAQPADRDDQPDI
                     LTPRERQVLELVARGYPNKSIAYELGISEHTAKFHVGSLLTKLNAASRAEVVTNATRR
                     GLLSV"
     gene            complement(1088..1486)
                     /locus_tag="VFI12_10335"
     CDS             complement(1088..1486)
                     /locus_tag="VFI12_10335"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HET7056851.1"
                     /translation="MPKAPVPDRYRDLLESTALGNLATINPNGRPQVNPVWFLWDGEH
                     VLLSIKPDTVKFRNLRANPRVAISVSDPARQNRYVELRGEAVDFELFDNLSWVNQLSR
                     KYTGADFTEGADGEHRYKVTIRIDSWTAQN"
     gene            complement(1511..1837)
                     /locus_tag="VFI12_10340"
     CDS             complement(1511..1837)
                     /locus_tag="VFI12_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056852.1"
                     /translation="MITGDHQFDLKPITEPVFDWKDHSRFVEIYPVSTGWLVLWGRYE
                     EMGAVRTLSGSRVYRSEAGARRRLAWAIIGLTRNPVDARDALRLLDRKGGLPSHRPRD
                     PHASIR"
     gene            complement(1837..2823)
                     /locus_tag="VFI12_10345"
     CDS             complement(1837..2823)
                     /locus_tag="VFI12_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7056853.1"
                     /translation="MAQHATTASATPAAAASHDPLDRMANKAHRSLWNNAWRQFRHHH
                     LAVTGLVIFLFFVLITTVGSAIYPRDKEYIPMDFSLWSLAPFKSWTFPLGTDSLGRDV
                     LARLLWGGRVSLAVGLISAAIGVTLGTLIGAASGFFGGFLDNLLMRFTDLFIALPQVP
                     LLLLVTFLFRDSIQSSFDRRFGNGDLGVFVLIVLVISLLSWMPTARLVRASFLSIKEK
                     EFVEAARSVGVSKTSIVFKHILPNALSPIIVAGTLSVGAAIITESVLSFLGLGFPPNV
                     PTWGRMLYEARDRLQTTPHEAMVPGTAIFLTVLAINYIGDGLRDALDPRKTQ"
     gene            complement(2839..3783)
                     /locus_tag="VFI12_10350"
     CDS             complement(2839..3783)
                     /locus_tag="VFI12_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7056854.1"
                     /translation="MLSYLVRRLFISAVTLVVISMVIFGILKVAPGDPLAGFANNPNV
                     PADVRLRLRKQLGLDDPIPVQYAKWAREYVRGNWGQSFLSRQPARDLIFSRLPITLEI
                     VGTAFLLSILIAVPIGVISAIKQYSTFDQVATTFAFLGYSLPTFFSGLVLIVIFSFKL
                     GWFPFVYDRTSQGLGAELKQIILPITVLALAGSASLTRFVRASMLEVISQDYVRTARA
                     KGLKEQGVIIAHAMRNAMIPVVTIIALQVPEVFGGAIITEQIFSVPGIGNLLIESIGS
                     ADHPVVMGISMGIAILVVLFNIIADVIYALLDPRIKLA"
     gene            3967..4269
                     /locus_tag="VFI12_10355"
     CDS             3967..4269
                     /locus_tag="VFI12_10355"
                     /inference="COORDINATES: protein motif:HMM:NF013545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056855.1"
                     /translation="MAAPLVLMAEPRTALGKKNGALRREGKVPGVVYGPGHDETIQVQ
                     VDRREFERFYVTNGLNKPFTLKWDGGSARVVIRDVQMNHLGTTPVHVDFYDDRKKK"
     gene            complement(4332..>4704)
                     /locus_tag="VFI12_10360"
     CDS             complement(4332..>4704)
                     /locus_tag="VFI12_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056856.1"
                     /translation="RHGQVALMRQVVVGDSDERVRAEMIADLMRLGELEQTATEVNRP
                     DRERQATEQYQRLIDENVFIAGEPETVARAILDARNTLGSSLLLANVYAAGIDQERVR
                     RTLTLLAGPVREQLDTLTLRI"
BASE COUNT          873 a         1412 c         1616 g          803 t
ORIGIN      
        1 cgtcgaggcg ctgtcgacgt atggtcgcct gcgccgcggg tacatcgggg tcggcgcgca
       61 ggcggtcggc ctgtcagcga gccaggcgca ggcggcgaag ctggatcgcg aagtcggtct
      121 cgtgatcctc tcgctcgacg aagatggccc ggcacagaag gcaggtttgc tgatcggaga
      181 tattctggtc aaggtgaacg gctcgccggt ggcggcggtg gatgatctgg ttgatcacct
      241 gtcgggtgat ttgatcggct cgacgatccc cctcgtcgtc gtccgggggg ccgggacgga
      301 ggatctgtcg gtcaccgtag gtgaacgtga ttagcacgac cgagcgcacc ggtacgagac
      361 gcacggatat tcggtcggcc gggacacttg cccggccggc tgcgtctccg cgtgtcgcgg
      421 tcttcgcgag ctcgcccgca ttgcgcgccg ggctgatcac cctgctgacg gaggatgatc
      481 gcgtcgacgt cctttcggac gacgcgctgg cccggggcga ggcgcccgac gtgatcctgc
      541 ttgagagcgg ggccggcgac ttgctcgacg agctgatcga cgacaactgg ccccgcagcg
      601 ccttgctctt cgtcggcgag ccgccggatg aggcgctggc ctccggcgat cgggtgaccg
      661 gagcggtctc ggccgggatc gagccagcgc gcttgagcgc ggcggtgcaa gcgatggcgg
      721 cggggctggc ggtgctcgat ccagacctgg aggccgcgct tcccctgcac gcgcagccgg
      781 cagaccggga tgatcagccg gacattctga cgcctcgcga gcggcaggta ctggagctgg
      841 tcgcgcgagg gtatccgaac aagtcgatcg cgtatgagtt ggggattagc gagcacacag
      901 ccaagttcca cgttggctcg ctcctgacca agctgaacgc cgccagccgc gctgaggtcg
      961 tcaccaacgc cacccgccgc ggcctcctca gcgtgtagca gccgggtcag cgcattccga
     1021 aacgtagcgg ggtggatgcc tgcgaccgcg tcctgatccc acggaaagca tgggcaaccg
     1081 tcaggctcta gttttgggcg gtccaggagt cgatgcggat ggtgaccttg tagcggtgct
     1141 cgccatcggc gccttcggtg aaatcggcgc cggtgtactt gcgggaaagt tggttgaccc
     1201 acgagaggtt gtcgaacagc tcgaaatcga ccgcctcgcc ccgtagctcc acgtaccggt
     1261 tctggcgcgc aggatcggat acggagatcg cgactcgcgg gttggcgcgc aggttgcgga
     1321 acttgaccgt atccggcttg atgctcagca aaacgtgctc gccgtcccac aggaaccaaa
     1381 ccgggttgac ctgggggcga ccgttcggat tgatcgtggc gaggttcccg agcgccgtgc
     1441 tctcgagaag gtcgcgatat cgatccggta ccggagcctt cggcatgata gctctccgag
     1501 tgcgaacagg tcagcgaatt gaggcgtgcg ggtcccgggg gcggtggctt ggcaatccgc
     1561 ccttccgatc gagcaggcgg agggcgtcgc gggcgtcgac cgggttgcgg gtcaggccga
     1621 tgatcgccca agccagacgc cgccgcgcac cggcctcgga tcggtagacg cgcgagccgg
     1681 acagcgtccg caccgcgccc atctcctcat accggcccca gagcacgagc catccggtcg
     1741 agacagggta gatctcgacg aagcggctgt gatccttcca gtcgaagacc ggctcagtga
     1801 tcggtttcag atcgaattgg tggtcgccgg taatcactat tgcgtcttgc gtgggtccag
     1861 ggcatcgcgc aggccgtcac cgatgtaatt gatggccagc acggtcagga agatggccgt
     1921 gccgggcacc atcgcctcgt gcggcgtggt ctgcaaccgg tcccgggctt cgtagagcat
     1981 gcggccccac gtcgggacgt tcggcgggaa gccgaggccg aggaaggaga ggaccgattc
     2041 ggtgatgatc gccgcgccga cgctgagcgt gccggcgacg atgattggac tgagcgcgtt
     2101 cgggaggatg tgcttgaaca cgatcgacgt cttgctgaca ccgacggagc gcgccgcctc
     2161 gacgaactcc ttctccttga ttgagaggaa gctggcccgg accagccggg cggttggcat
     2221 ccagctcagg agcgagatga cgagcacgat caagacgaag acgccgaggt cgccgttgcc
     2281 gaatcgccgg tcgaacgaac tctggatcga gtcacggaac aggaaggtca cgagcaggag
     2341 gagcggcacc tgcggcaggg cgatgaacag gtcggtgaac cgcatcaaca gattgtcgag
     2401 gaagccgccg aagaagccgg aggccgcgcc gatcagggtg ccgagcgtga caccgatcgc
     2461 cgccgagatg agaccgaccg caagcgatac gcgcccgccc caaagcagcc gcgccaagac
     2521 atcgcgtccc agcgagtcag tcccgagcgg gaaggtccag gacttgaacg gcgcaaggct
     2581 ccagagcgag aagtccatcg ggatgtattc cttgtcccga ggatagatcg cgcttccgac
     2641 ggtcgtgatg agcacgaaga agaggaagat caccaagccc gtaacggcca gatggtgatg
     2701 gcggaactgc cgccaggcgt tgttccagag tgagcgatgc gccttgttgg ccattcggtc
     2761 gagcgggtcg tgggatgcgg cagcggccgg cgtggccgac gcggtggttg cgtgctgtgc
     2821 catcggtaac gctccctctt acgccaactt gatccgcggg tcgagcaacg cgtagatgac
     2881 gtccgcgatg atattgaaga gtacgacgag gatcgcgata cccattgaaa tccccatgac
     2941 gaccgggtga tcggcgctgc cgatggactc gatcagcagg ttgccgatgc ctggcacgct
     3001 gaagatctgc tcggtgatga tcgctccgcc gaagacctcc ggcacctgca aggcgatgat
     3061 cgttacgacc ggaatcatcg cgttgcgcat cgcgtgggcg atgatcacgc cttgctcctt
     3121 gaggcccttg gcgcgggcgg tacgcacgta atcctggctg atcacctcga gcatcgatgc
     3181 gcgcacgaac cgggtcagtg atgcggaacc ggccagggcg aggacggtga tgggcaagat
     3241 gatctgtttg agctccgcgc ccaagccttg cgacgtccgg tcgtagacga aggggaacca
     3301 gccgagttta aagctgaata tgacgatcag gaccaggccg gaaaagaacg tgggcaaact
     3361 gtacccaaga aaggcgaagg tcgtggcgac ctggtcaaag gtcgagtatt gcttgatcgc
     3421 cgatatgaca ccaatcggga ccgcgatcag gatactgagc aggaacgcgg tgccgacgat
     3481 ctcgagcgtg atcggcaagc ggctgaagat gaggtcgcgg gcgggttggc gggaaaggaa
     3541 cgactggccc cagttgccgc gcacgtattc ccgcgcccat ttggcgtact ggacaggaat
     3601 cgggtcatcc agccccagct gcttgcgcag ccgcaggcgc acgtccgccg ggacgttcgg
     3661 attgttggcg aaaccggcca gtgggtcgcc gggggcgacc ttgaggatcc cgaaaattac
     3721 catgctgatg acgaccagcg tgaccgccga gatgaacagg cgccggacca ggtaactgag
     3781 catctaatcc cctccgcttt gtctcatgtt cgcggaatga ggtgaatcca gtctcgagac
     3841 gagatggcga accataccct tgacgcgcgc aacgcgtcta cccggtttcc gcattgcatg
     3901 ctaaagttgc ctggcgcgcg acacgggtcg cgctttttca tggtgtaggt agtaggagtc
     3961 aatcgggtgg cagcaccatt ggttctcatg gccgagccgc gcactgccct gggcaagaag
     4021 aacggcgcgc ttcggcggga aggcaaagtc cccggcgtcg tctatggccc cgggcacgac
     4081 gagaccatcc aggtccaggt cgatcgccgc gagttcgagc ggttctacgt gacgaacggg
     4141 ctgaacaagc cgttcacgct caagtgggat ggcggttccg cgcgtgtcgt gattcgcgac
     4201 gtgcagatga accaccttgg cacaacgccg gtccacgtcg atttctacga cgatcggaag
     4261 aagaagtaac cggcgttccg cgcgacgaac acaatcggcc tcttgcctga tccaggcgag
     4321 gggccgattt gctatatgcg aagcgtgagc gtgtcgagct gctcccggac tggtcccgcc
     4381 aggagcgtca aggtgcgccg gacccgctcc tgatcgatgc ccgcggcgta gacgttcgcc
     4441 agcagcaggc tggagccgag cgtgttccgg gcatcgagga tcgcccgggc caccgtctcc
     4501 ggttccccgg cgataaagac gttctcgtcg atcaaccgct ggtattgctc ggtcgcctgg
     4561 cgttcgcggt cagggcgatt cacttcggtg gcggtctgct cgagctcgcc gagccgcatc
     4621 agatcggcga tcatctccgc ccgcacccgc tcatctgaat ccccgacgac gacctgccgc
     4681 atcagggcga cctggccgtg acga
//
LOCUS       DASSGX010000570         3740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1189926, whole genome shotgun sequence.
ACCESSION   DASSGX010000570 DASSGX010000000
VERSION     DASSGX010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189926"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..730)
                     /gene="recR"
                     /locus_tag="VFI12_10365"
     CDS             complement(119..730)
                     /gene="recR"
                     /locus_tag="VFI12_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HET7056857.1"
                     /translation="MQIIRSRGPAAPVARLIEELGKLPGVGPKTASRLAFHLLRTSRE
                     EAIALSDAIVEVRSKIKLCSRCFNITESDPCPICGDPERESAICVVEEPLDVVALERT
                     GQLHCRYHVLHGAISPIDGIGPDQLRIRELVNRVESERPDEVILATNLDLPGDATATY
                     LHRMLGPTGVRITRPASGLPVGGDLEYADEQTLGRALMARRVL"
     gene            complement(832..2871)
                     /locus_tag="VFI12_10370"
     CDS             complement(832..2871)
                     /locus_tag="VFI12_10370"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HET7056858.1"
                     /translation="MIGLVTAWSVLAGESNGILVAGLDRFLAWLCGRAPYVIPVLAML
                     LSYQCFRNRQGQVVRLRQLAGGIAFGLSVVGLTHAAATGTRSERGGLIGGFVFDYTQM
                     LVGDYGAGVGLFLIGVVAVLVTTNTNPMNLYREVERIGRAIYKPLPPIEDTDLFDTIP
                     AIAEESANVVAREPEPIPAAAPSLVINSPKKVSIVKKGREAVTRVVAGPKPSPEILAL
                     PDISTWHLYDQITLDKGDLTAKARRIEETLGSFKVEARVREINPGPAVTQFTLEPGLG
                     TKVRRITELQNDLALALAAPSIRIEAPVPGLARVGLEIPNAKVATVGLRETLESERFT
                     NGKFKLPVPLGRDVNGKYVIHDLTKMPHLLIAGATGSGKSVCLTAIISTFLSSKQPNE
                     LKMLMMDPKMVELVGYNGVPHLQCPVVTEMDKVVGALRLVLQEMERRYTVFSKLGVRN
                     LDGYRLKIADEPGAEEIPYLVVIIDELADLMMTAPDDVETVLCRLAQMGRAAGIHLII
                     ATQRPSVDVLTGLIKANVPARVAFAVTSLTDSRVVLDMPGAERLLGRGDMLFLPPDAA
                     KPVRIQGAFIEDGDVQGIVDHWHTVSPVPAYAPEWLDLPSSSTPPGELDDSSDDPLLD
                     QAISIVKSQGTASASMLQRRLRIGYNRAARLIEQMEADGLIGPADGIKGRPVLDA"
     gene            3320..>3740
                     /locus_tag="VFI12_10375"
     CDS             3320..>3740
                     /locus_tag="VFI12_10375"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET7056859.1"
                     /translation="MTRLLLRNARQIDPAIALDQFGDLLIEDGRVAAVSTGAIAEPAD
                     RVIDCTGYVVSPGWIDMHVHLRVPGQEHKETIETGTAAAAAGGFTTIAVMPNTEPALD
                     SAQTIQWLLDECRKRAVVNVLPIAAITVGRAGQTLVDF"
BASE COUNT          653 a         1217 c         1179 g          691 t
ORIGIN      
        1 ggcgtggtcc gacgcgaccg gaagacggtc gcctgatcga ttgaggtgcg gaacgcgaac
       61 ctgtgccggc tgttcgatca tgtgcgccca ttgtagggcg actgtcaccc ccgggcaatc
      121 acaagacccg ccgggccata agtgcgcgtc caagcgtctg ctcgtccgca tattcaaggt
      181 ctccaccaac cgggagcccg ctggccggcc gcgtaatccg gactccggtt gggccgagca
      241 tgcggtgtag atatgtcgcg gttgcgtccc cgggaaggtc gagattcgtg gcgagaatca
      301 cctcgtccgg ccgttccgat tcgacgcggt tgaccagctc cctgattcgg agctgatccg
      361 gcccgatccc gtcaatcggc gaaattgccc cgtgcaagac atggtatcga cagtgcagtt
      421 ggccggttcg ctccagggct acgacgtcca acggctcctc gacgacgcag atcgccgact
      481 cgcgctccgg atcgccgcag atcgggcagg gatccgactc cgtgatgttg aagcaccgcg
      541 agcagagctt gatcttgctc cgcacctcga caatcgcatc ggacaacgcg atcgcctcct
      601 cgcgggatgt ccgcaggagg tggaaggcca gccgggacgc ggtctttggt cccacacctg
      661 gcagcttgcc gagctcttcg attaggcgcg caaccggggc ggcggggccc cgggaacgga
      721 ttatttgcac tgggattcct caggattgtt ctggcccgaa aaactcggac acgtgttcga
      781 ttttaccatc tatggggtcc cgattcgagc ctggggcggg ctgaatcccg gctaggcgtc
      841 gaggaccggg cggcccttga tgccgtccgc cgggccgatc aggccgtccg cttccatctg
      901 ctcgatcagc cgagcggcgc ggttgtagcc gatccgcaat cggcgttgga gcatggatgc
      961 agaagccgtt ccctggctct tgacgatcga gatcgcctgg tcgagcagcg ggtcgtcact
     1021 cgagtcgtcc agctcgccgg gcggggtcga gctgctcggc aaatcgagcc actcgggtgc
     1081 atatgccggc accggcgata cggtatgcca gtgatcgacg atcccctgca cgtcgccgtc
     1141 ctcgatgaat gcgccttgaa tccggaccgg cttggccgcg tccggcggta agaaaagcat
     1201 gtcgccccgt cccagcaacc gctccgctcc cggcatgtcg agcacgacgc gactatcggt
     1261 cagcgatgtc accgcgaacg caacccgcgc cggtacgttg gccttgatca agccggtcag
     1321 gacatcgacg gacggccgct gcgtggcgat gatcaggtga atccccgccg cccggcccat
     1381 ctgcgcgagc cggcagagca ccgtctcaac gtcgtccggc gcggtcatca tcaggtcggc
     1441 cagttcgtcg atgatgacga ccaggtacgg aatctcttcc gcgcccggct catcggcaat
     1501 cttcaatcga tagccgtcca ggttgcgcac gcccaacttc gagaaaaccg tgtagcgccg
     1561 ctccatctcc tgcagcacca accgcagcgc gcccacgacc ttgtccatct cggtcacgac
     1621 cggacattgc aggtgtggca cgccgttgta gccgaccagc tcgaccattt tcgggtccat
     1681 catcagcatc ttcagttcgt tcggctgctt ggacgagagg aacgttgaga tgatcgcggt
     1741 caggcacacc gacttgccgc tgccggtcgc cccggcaatc agcagatgcg gcattttggt
     1801 caggtcgtgg atgacgtact tgccgttgac gtcccgcccg agtggaaccg gcaacttgaa
     1861 cttgccattc gtgaagcgtt cggactcgag cgtttcgcgc aggccgaccg tcgcgacctt
     1921 tgcattcgga atctcgagac ccacccgcgc caatcccggc accggcgcct cgattcggat
     1981 gctcggcgcg gcgagtgcca gggcaaggtc gttctgcagt tcggtgatcc ggcgaacctt
     2041 ggtgccaagg ccgggctcga gcgtgaattg cgtcacggcc ggcccggggt tgatctcacg
     2101 gacgcgcgcc tcgaccttga agctaccgag ggtctcctcg attcgcctcg cctttgcggt
     2161 caggtcgccc ttgtcgagcg tgatctgatc gtagaggtgc catgtgctga tgtcgggcag
     2221 ggcgagaatt tcggggctcg gctttggacc ggcgaccacc cgagtgactg cctcgcggcc
     2281 cttcttgacg atggatactt tttttgggct gttgatcacc agggagggcg ccgcagccgg
     2341 gatcggctcc ggctctcgcg cgaccacgtt cgccgactcc tcggcgatgg cggggatcgt
     2401 gtcgaacagg tcggtgtcct caatcggcgg cagcggctta tagatcgccc ggccgatccg
     2461 ctccacctcg cggtacaggt tcattgggtt cgtgttggtc gtgaccagta ctgccacgac
     2521 gccgatcaga aacaggccga cgcccgctcc gtaatcaccc accagcatct gcgtgtagtc
     2581 gaagacgaag ccgccgatca gcccaccgcg ctcgctccgc gtgccggtgg cggcggcatg
     2641 agtcagaccg acgactgaga gaccgaaggc gatcccgccg gcaagctggc gaaggcggac
     2701 gacctgcccc tgccgattcc ggaagcactg atacgagagc agcatcgcga gaacgggaat
     2761 cacgtacgga gcgcggccgc aaagccacgc caggaaccga tcgagcccag cgacgagaat
     2821 cccgttcgac tcgcccgcca gaacggacca tgcggtgacg agcccgatca acgcggatac
     2881 cgcgccgacg aggtcgcggt tgaagattga tgccgaggga acgagcccac tcaggttcgg
     2941 gatacgactc tgcgcaacgc tcagcgcgct ctgccgtgag tccgagccct tcctttgttt
     3001 cgacttgttt ggcttcgctc ccgatcggag cggccgtcca gcagcgcgag tcgccattcg
     3061 tatgccattg catgcagcgc cgattgccgg gcgcaccggt cgcggggtgt acgtacggga
     3121 gcttcattgt acacccatct acccagaatt acgcaagtcc tgcttttgac cgggaccgtc
     3181 aggaacggct atactgggtg tctggagtga gaggaacccc gcttttgcca tcgccccaga
     3241 tccagagtcc caaacggaag agtgcgcctc gggatcattc gatttcgggg tctttttttg
     3301 cgcggaggga gactaccgca tgacgcgcct cctgctgcga aacgctcgtc agatcgatcc
     3361 cgccattgcc ctggaccagt tcggtgacct cctgatcgag gatggtcgag tcgccgctgt
     3421 ctcaaccggc gcgatcgcgg agccggcgga ccgcgtaatc gactgcacgg gatatgtcgt
     3481 ttctccgggc tggatcgaca tgcatgtcca cctccgagtg cccggccagg agcacaagga
     3541 gacgattgag accggcaccg cggccgccgc ggccggcggc ttcaccacca tcgcggtcat
     3601 gccgaacacc gagccggcac tcgattcggc ccagactatc cagtggctgc tcgacgagtg
     3661 tcgcaaacgc gccgtggtga acgtgcttcc gatcgccgcg atcaccgtcg gccgggcagg
     3721 acagacgctt gtcgatttcg
//
LOCUS       DASSGX010000571         5658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_101078, whole genome shotgun sequence.
ACCESSION   DASSGX010000571 DASSGX010000000
VERSION     DASSGX010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5658
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101078"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..1133
                     /gene="mraY"
                     /locus_tag="VFI12_10380"
     CDS             96..1133
                     /gene="mraY"
                     /locus_tag="VFI12_10380"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HET7056860.1"
                     /translation="MNTDLTLTLPLLTSSIMPVEPNENMAVSLGLAGLAFMLTLIVGR
                     PIVTYLRLKNIGKQVRIDGPKTQLWKTGTPTMGGIMISIVVILITAIFNVAGRLSMLL
                     PIGMLLAAAILGAVDDRMNLVGGKKTGMTAKFKMAWLLLFSIVAASLLHIPRVGLGLH
                     HVYVPFLGRFDISWFYLPVAVFVIVGTANAVNFADGLDTLAAGLAAIAFVAYGVIAYR
                     QGQVGVVTFCFTMVGSLLGFLWFNAHPAQVFMGDTGSLAIGSALAVAAFMTGQWLLLP
                     VVGGVFVAEAVSVILQVGYFKLTGGKRIFKMTPLHHHFEQSGWSETQITMRFWIVGMM
                     CGLIGVALALL"
     gene            1178..2539
                     /gene="murD"
                     /locus_tag="VFI12_10385"
     CDS             1178..2539
                     /gene="murD"
                     /locus_tag="VFI12_10385"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HET7056861.1"
                     /translation="MGLGTRAGGAGVARYLAESGAIVTVTDLRDESALAQPMAELDGL
                     GIRFVLGHHEPADFTDTDVVVRNPGVRRTNQYLAMAREAGVEIDMEMAIFLRASPAPV
                     IGITGTKGKTTTSALCGEMLRHWRPETVVAGNMGVSAVSALLEITPETPVVLELSSWQ
                     LEGMHERQIGPHIAVITNISEDHLDTYRDFDEYADTKRSLGSHLAANDYLVLNAADPE
                     VARAAEHTAARVMWFGAGELPGVGVRAHTNALISTIPGREGRIAMPDNPALRGEHQRV
                     NAAAAACAALLRGASIAGVAAGLASFTGVANRMEIVAEVDSVLFVNDTAATAPAAAIA
                     SLRAFSDRPIHLICGGADKRLDFRPLAQEIVACVSSVTLLDGTATAALRSLIEAAGME
                     VGAPVQTMREAVEQAIRHARAGDVVLLSPGCASFGLFRDEFDRGSQFRHAVLARAAVG
                     AGQ"
     gene            2536..3909
                     /gene="murC"
                     /locus_tag="VFI12_10390"
     CDS             2536..3909
                     /gene="murC"
                     /locus_tag="VFI12_10390"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HET7056862.1"
                     /translation="MSQPSGLPQPPAHVHFVGIGGIGQSGLARILTAWGYRVTGSDAF
                     PSELVEQLRLEGIDVQIGHDETANATAADLIVATAAVRATNSEIHAAKARGTPVIKRA
                     ELLGMLANARTCVAVAGSHGKSTTSGMLVTALQATGRDPSYAVGAVIGSTGTNAAPGS
                     GEAMVVEADEYDYSFLWLKPELAIITNVDYDHPDLFPDQATYDRAFVRFAQGVRPGGV
                     LVVNGDDPGVQRILPSLRTQPARVVTYGRSSGGNWRFDGAAVTGPDGQRIDLNLSVPG
                     AHNLSNATAALIVMREMGLPLSQAAAGLAGYRGVGRRFDLKGEASGVTVIDDYAHHPS
                     EIAATIAAARERYPDRRIIAAFQPHTFSRTRALLEPFAETLSAADRAVVLDIYPARET
                     DTLGVSSADIARRMAPESTSLGGKPAQAVKQLANMVQAGDVILTLGAGDITLVGPKLL
                     ALLEERS"
     gene            3906..4871
                     /gene="murB"
                     /locus_tag="VFI12_10395"
     CDS             3906..4871
                     /gene="murB"
                     /locus_tag="VFI12_10395"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HET7056863.1"
                     /translation="MTARTSNISAIQIPDAPHLRVEQDSPLSRTTTWRIGGPADFLVR
                     TGTSDDLTAAVHWGVGEGLPITVIGGGSNLLVSDAGIRGLVIISKVSGEKATNLVTWD
                     DLGDEVRLTVGAQAPLSWVGRFASENGWAGMDWGVGLPGTIGGATVNNAGAHGTEQKD
                     HLESLTVLRLDSAVESHDRAWLNPTYRNTTIKAMSRPRDYVVVDVTMRLPRGDTAELV
                     RLSDKHAAYRKATQPTGACGGSTFANPDGDFAGRLLEVAGLKGFGIGPVSFSEKHTNF
                     IINAGGGTAEQVRELIAHGQAVVRERFGVELHREIEYLGEMAGAE"
     gene            4868..>5658
                     /locus_tag="VFI12_10400"
     CDS             4868..>5658
                     /locus_tag="VFI12_10400"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HET7056864.1"
                     /translation="MTSNKAKINVLVLFGGQSGEHDVSLRSAQTVIGALGDRYAVRQV
                     GITREGGWIASGDPMAALTASSPLFQIGDGQAPPAEVQAEPGLPTLARDSVDVVFPVL
                     HGPMGEDGTIQGMLELAGIPYVGSGVLGSSVAMDKAMTKLVLSQAGIPQAKWALVNRF
                     EWEADADCVVESIITTLGLPCFVKPANMGSSVGVTKAHDEAELRQAIDFAQEYDRRVV
                     IEEAVDGRELEISVLGNDQPVASVAGEIVPCNEFYDYDAKYVDDDS"
BASE COUNT          938 a         1772 c         1933 g         1015 t
ORIGIN      
        1 cttctgcagg agccggtgga cggcgacgtc gttctgctca aaggttcccg cggactcgaa
       61 atggagcgca tcgtgaccgc cttgcagccg gcgccgtgaa tacggatttg accctgacac
      121 tcccactcct gaccagctcg atcatgccgg tggaaccgaa cgagaacatg gcggtctcgc
      181 ttggactggc cggtctcgcc ttcatgctga cactgatcgt cggtcgcccg atcgtcacat
      241 atcttcgcct caagaacatc ggcaagcagg tccgaatcga cgggccaaag acgcagctct
      301 ggaaaaccgg cacgccgacg atgggcggca tcatgatctc gattgtggtc atcctgatca
      361 cggccatctt caacgtcgct ggccggctct cgatgttgct cccaatcggg atgttgctgg
      421 cggcggcgat tctcggcgcg gtcgacgacc gcatgaacct ggtcggcggc aagaagaccg
      481 ggatgacggc caagttcaag atggcctggc tactgctctt cagcatcgtg gcggccagcc
      541 tgcttcatat accgagggtc gggcttggcc tgcaccacgt ctacgtgccg ttcctcggcc
      601 gattcgatat ctcgtggttc tatctgcccg tcgccgtctt cgtcatcgtc ggaacggcca
      661 atgcggtcaa ctttgccgac gggcttgata ccctcgcggc cggattggcg gcaatcgcgt
      721 tcgttgcgta cggggtgatc gcgtaccggc aagggcaggt cggcgtcgtg accttctgct
      781 tcacgatggt cggcagcctg ctcggctttc tctggtttaa tgcccatccg gcgcaggtct
      841 ttatgggcga taccggatcg ctcgcgatcg gctcggctct ggcggtggcg gcgttcatga
      901 ccgggcagtg gttgctgttg ccggtcgtcg gcggtgtctt cgtggccgag gccgtctcag
      961 tcatcctcca ggtcggctat ttcaagttga ccggcggcaa gcgcatcttc aagatgacgc
     1021 ccctccacca ccacttcgag caaagcggct ggtctgaaac gcagatcacc atgcgctttt
     1081 ggatcgtcgg catgatgtgc ggcctgatcg gggtcgcgtt ggcgttgctc tgatggcggg
     1141 gcacgggacg gactgggccg ggcgacgggt gaccgtcatg ggtctgggta cgcgcgccgg
     1201 cggagccggc gtggcccgat acctcgccga atcgggcgcg atcgtcaccg tcacggacct
     1261 gcgagatgaa tcggcgcttg cccaaccgat ggcggagctt gatgggctcg gcatccggtt
     1321 cgtgctcggc caccatgagc cggccgattt cacggatacc gatgtggtcg tgcgcaatcc
     1381 cggtgtacgc cggacgaatc agtacctggc gatggcccgg gaagccggcg tcgagataga
     1441 tatggagatg gcgatcttcc tgcgggccag cccggcgccg gtaattggca tcaccggcac
     1501 caaggggaaa acgacgacca gtgccctctg tggcgagatg ctgcgccact ggcgaccgga
     1561 gacggtcgtc gccggcaaca tgggcgtctc agcggtttcg gccttgctgg agatcacgcc
     1621 ggagacgccg gtcgtgctcg agctgtcgag ctggcagctc gaaggcatgc atgagcgtca
     1681 gattgggccg catatcgcag tgattacgaa catttcggaa gaccacctcg acacgtatcg
     1741 ggatttcgac gaatacgccg atacgaagcg ctcgcttggt agccacctcg ctgcgaacga
     1801 ttacctcgtg ctgaacgccg ccgatccgga ggttgccagg gcagcggaac ataccgcagc
     1861 gcgggtgatg tggttcggcg ccggcgaatt gcccggtgtc ggtgtccgag ctcacactaa
     1921 cgcgctcatt tcgacgattc cgggacgcga gggccggatt gcgatgccgg acaatcctgc
     1981 cctccggggc gagcaccagc gcgtgaacgc ggcggctgcg gcctgcgcgg cactgctgcg
     2041 gggtgcgtcg attgccggcg ttgccgctgg actggcgtcg ttcaccggcg tcgcgaaccg
     2101 gatggagatc gtcgcggagg tcgatagcgt cctcttcgtg aacgacaccg ctgccaccgc
     2161 accggccgcc gccattgcca gcctgcgcgc gttcagcgac cggccgatcc acctgatctg
     2221 cggtggcgcg gacaagcgac tcgacttcag gccgctcgcc caggagatcg tcgcgtgcgt
     2281 gtcgtcggtc acgctgctcg acggtacggc aaccgccgcg cttcgctcgt tgatcgaggc
     2341 ggcgggcatg gaggtcgggg cgccggttca gacgatgcgc gaggcggtag agcaggcgat
     2401 acgccatgcg agggcgggtg acgtcgtgct gctctcgccc ggatgtgcca gcttcgggct
     2461 gttccgggac gaattcgatc gtggctcgca attccggcac gcggtcctgg cccgcgctgc
     2521 cgtgggagcc gggcaatgag ccagccgagc ggattgccgc agccaccggc gcatgtccat
     2581 ttcgtcggca tcggcgggat cgggcagagc ggacttgccc gcatcctgac cgcgtggggc
     2641 taccgggtca cggggtccga cgcctttccg tccgagttgg tcgaacagct ccgcctcgag
     2701 gggatcgacg tccagatcgg gcatgacgag acggcgaatg cgaccgccgc ggacctgatc
     2761 gtagcgacgg cggcagtccg ggctacgaat tccgagatcc acgcggcgaa agcgcgcggg
     2821 acgccggtca tcaagcgggc cgagctgctc gggatgctgg caaacgcccg gacctgcgtc
     2881 gcggttgccg gctcgcacgg caaatcgacg accagcggca tgctcgtgac ggcattgcag
     2941 gcgaccggtc gcgatccgtc ctacgcagtc ggcgcggtca taggcagcac cgggacgaac
     3001 gccgcgccgg gatcgggcga ggcgatggtg gtcgaggcgg acgagtacga ctactcgttc
     3061 ctctggctca agcccgaact cgcgatcatc accaatgtcg actacgacca cccggacctg
     3121 ttccccgacc aggcaaccta cgatcgcgcc tttgtccgtt tcgcgcaggg ggtgcgaccg
     3181 ggtggcgttc tagtcgtcaa cggcgacgat cccggcgtcc agcggattct cccgagcctt
     3241 cgaacgcagc cggcgcgggt cgtcacgtac ggtcgatctt ccggcggcaa ctggcgcttt
     3301 gacggcgctg cagttacagg gccagatgga cagcgcattg atctcaatct atccgtgccc
     3361 ggcgcgcaca atctctcgaa cgccacggcc gcgctgattg ttatgcgcga gatgggcctg
     3421 cccctgtccc aggccgccgc cgggctcgcc ggataccggg gcgtcgggcg gcggttcgac
     3481 ctcaagggcg aggcatctgg cgtgaccgtg atcgacgact acgcccacca cccgagcgag
     3541 attgccgcga ccatcgccgc cgcccgggag cgataccctg accgtcgcat catcgccgcc
     3601 ttccagcccc acacgttcag tcgcaccagg gcgttgctgg agccgttcgc ggagacgctt
     3661 tcggcggcag atcgggccgt ggttctcgac atttacccgg cgcgcgaaac cgatacgctc
     3721 ggcgtttcgt cggcggatat cgcgcgacgc atggctccgg agtcgacctc gctcggtggc
     3781 aaaccggcac aggccgtgaa gcaactggcg aacatggttc aggctggcga tgtgatcctg
     3841 acccttgggg cgggcgatat caccctcgtc ggcccgaagc tgctggccct gttggaggaa
     3901 cgatcgtgac tgcgcggacg agcaacattt ccgctatcca aattcccgac gcgccgcatt
     3961 tgcgagtcga gcaggattcg ccgctttccc ggacgacgac ctggcgaatt ggtggaccgg
     4021 ccgactttct ggtccgtaca gggacctctg acgacctgac ggctgcggtc cactggggcg
     4081 tcggcgaagg gttgcccata actgtcatcg gtggtgggtc gaacctactc gtctccgatg
     4141 ccgggattcg cgggctggtc atcatctcga aggtctctgg cgagaaggcg acgaacctgg
     4201 tgacatggga tgacctcggc gatgaggtcc ggttgacagt cggcgcgcag gctccgctga
     4261 gctgggtcgg ccggttcgcc agtgagaacg gctgggccgg gatggattgg ggcgtcggcc
     4321 ttccgggcac gatcggcggc gccacggtca ataacgccgg cgcgcacggg actgagcaga
     4381 aggaccacct cgaatcgctg accgtacttc ggctcgatag cgcggtcgag tcccacgatc
     4441 gcgcgtggct gaacccgacc taccgaaaca cgacgatcaa ggcgatgtcc cgtccgcgcg
     4501 actacgtcgt ggtcgatgtc acgatgcggc tgccgagagg cgacacggcg gagttggtcc
     4561 gcctttccga taagcacgct gcctaccgca aggcgacgca gccgaccggc gcctgcggcg
     4621 gctcgacctt cgccaacccg gatggcgact ttgccggtcg attgctcgaa gtggccggtc
     4681 tcaagggctt cggaatcgga ccggtttcat tctcggagaa gcacacgaat ttcatcatca
     4741 acgcgggtgg cggcacggcc gagcaggtcc gggagctgat cgcgcacggg caagcggttg
     4801 tgcgcgagcg gtttggtgtc gagctgcacc gcgaaatcga gtatctcggc gagatggcgg
     4861 gggcagaatg acgagcaaca aggcgaagat caatgtgctg gtgctgttcg gcgggcaatc
     4921 cggcgagcac gatgtctcgc tgcggtccgc gcagacggtg atcggggcgc tcggcgaccg
     4981 gtacgcggtc cggcaggtcg ggatcacgcg ggaaggcggc tggattgcct ccggcgaccc
     5041 gatggccgcg cttactgcct cgtcaccgct cttccagatt ggtgacggcc aggcgccgcc
     5101 ggccgaggtc caggccgagc cgggcttgcc gacgctcgcg cgcgattcgg tcgacgtcgt
     5161 cttcccggtc ctgcatgggc cgatgggcga agacggcacg atccagggca tgcttgagct
     5221 tgcgggaatc ccgtatgttg gctccggcgt gctgggttca tcggtggcga tggacaaggc
     5281 gatgaccaaa ctggttctca gccaggccgg catcccgcag gcgaagtggg cgctggtcaa
     5341 tcgcttcgag tgggaagcag atgcggactg cgtcgtggaa tcgatcatta ccaccctcgg
     5401 tctcccgtgc ttcgtcaagc cggccaacat gggctcgagc gtcggagtca ccaaagcgca
     5461 tgatgaggcg gagttgcgcc aggcgatcga cttcgcgcag gaatacgacc ggcgcgtggt
     5521 catcgaggag gcggtcgatg gccgcgagct ggagatatcg gtactcggca acgaccagcc
     5581 ggtggcgtct gtcgccggcg agatcgtgcc ctgcaacgag ttctacgatt acgacgccaa
     5641 gtacgtggac gacgactc
//
LOCUS       DASSGX010000572         2230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1626533, whole genome shotgun sequence.
ACCESSION   DASSGX010000572 DASSGX010000000
VERSION     DASSGX010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1626533"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VFI12_10405"
     CDS             complement(<1..261)
                     /locus_tag="VFI12_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056865.1"
                     /translation="MSEDRPGNDDQKAQTNQNPEQHNLTPSRNIQSHQQHGDLTAADV
                     RGTIPEHAGPEPTSAKESTDRVVNRTLYPHPIFACARCSGKLR"
     gene            339..1136
                     /locus_tag="VFI12_10410"
     CDS             339..1136
                     /locus_tag="VFI12_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056866.1"
                     /translation="MTDVARPVLSATVHDPDGRFLPLLSHVAASLAWYRSAAFAVTDE
                     TDARIATALESLGAEIIQVATGAIGTARRAALRRSAERCHEAGILACDFDRWLHWARH
                     YSRELRCVPERIAAIRPAPWYVCIGRSARAFATHPQVQRDAEAATNHALEIVVGFPID
                     AVAGACWLSPEGLSQVLDHSCEATAATDLEWPAIVHRREAARLAAIQVEGLEFETPSF
                     YQDQVVLAGSVERWIGQTYERPEMWQARLQLAADSVAALTREMTPDQ"
     gene            complement(1155..>2230)
                     /locus_tag="VFI12_10415"
     CDS             complement(1155..>2230)
                     /locus_tag="VFI12_10415"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET7056867.1"
                     /translation="ALTRIECSVQINRRRLIAIAVSAATTPVSIRLSAAQEATPAASP
                     IPVQKLLDTSAPETGPVRAARSYRLRLLVPFKDDPFWRSVQNAVAGRATADGVTVDIV
                     PLSAPSVPEQLSQIDDAITNQVDGILLGPIDAAAIAPGIASANVAGIPVIAIDTAPTA
                     GQVVSVVRTDNVAAARRAGLYIGQKLDGKGSVLNVQGDLANPVGQERDTGFRDGLSGF
                     PDITLVSAPGSWQYNNGLFQTLAQLPQPQKGTPTPPEPLVNAVFAAGPEMARGAADAV
                     EQVQADTVFVVGFGATNDTLRMIRNGLLDAVVAEYPTRAGAMAVDQMVRHLNGESIPM
                     QMDSGSTLVNSGNIDAFVASGAQ"
BASE COUNT          390 a          699 c          711 g          430 t
ORIGIN      
        1 ccgcagctta cctgaacaac gagcgcaggc aaatatagga tgcgggtata gcgtgcggtt
       61 gacgactcga tctgtcgact ccttggccga cgttggttca ggcccggcat gttcaggaat
      121 ggttccgcga acgtcagctg ccgtaagatc gccatgttgc tgatgagact ggatgtttcg
      181 tgaaggcgtg aggttgtgct gctctggatt ctgattggtc tgggccttct gatcatcgtt
      241 gcccggacgg tcttcggaca ccgtgtggcg ctttcgctgg tagcgctatg ggttgcggtc
      301 gctgtcatcg ggtgggcgac cggcatcggt gtccgccggt gaccgacgtc gcccggcccg
      361 tactttccgc cactgtccac gaccccgacg gccggttctt gccactgctt tcccacgttg
      421 ccgcgtcgct cgcctggtat cgatctgcgg catttgccgt gacagatgaa acggacgcgc
      481 gcatcgcgac cgcactcgag tccctcggcg cggagatcat ccaggtagcg accggcgcga
      541 tcgggaccgc gcgccgagca gcgttgcgtc gctcggctga gcgctgccac gaggcgggaa
      601 tactggcatg cgatttcgac cgctggctgc attgggctcg gcactactcc cgggaacttc
      661 ggtgcgttcc cgagcgcatc gccgcgatca ggcctgcccc gtggtatgtc tgcatcggac
      721 gaagcgcacg agccttcgcc acgcatccgc aagtccaacg cgacgcggag gccgccacga
      781 atcacgcgct ggagatcgtg gtcgggttcc cgatcgacgc ggtcgctggt gcgtgctggc
      841 tctcgccgga gggactctct caggtgctcg accactcttg tgaagccacc gccgcgaccg
      901 atctcgagtg gccggcgatc gttcaccggc gggaggccgc ccgcctggcg gcaattcagg
      961 ttgaaggtct cgaattcgag acgccaagct tctaccagga ccaggtcgtc ctcgccggaa
     1021 gcgtcgaacg gtggattggg caaacctacg agcgacccga aatgtggcaa gctcgcctgc
     1081 aacttgccgc agattccgtt gccgcgttga cccgggagat gacgcctgac caatgaacgt
     1141 cggagcgggc agcgttactg agcgccggaa gcgacgaacg cgtcgatgtt cccactgttg
     1201 acgagtgttg acccactgtc catttgcatt ggaatagact cgccattgag gtgccgtacc
     1261 atctggtcga ccgccatcgc acctgccctg gtcgggtact ccgcgacgac agcatcgagc
     1321 aacccattgc gaatcatacg gagcgtgtcg ttggtggcgc caaatccgac cacaaagacc
     1381 gtatcggcct gaacctgctc gactgcgtct gcagcccccc tggccatctc gggcccggcg
     1441 gcaaagacag cgttgacgag cggctctggc ggcgtcggtg tccctttctg cggctgtggg
     1501 agctgggcaa gcgtctgaaa gaggccgttg ttgtactgcc agcttccggg cgctgagacg
     1561 agagtgatgt ccggaaatcc cgacaatccg tctcgaaagc ccgtatcgcg ctcctggccg
     1621 accggattcg ccaagtcgcc ctggacgttg agcaccgaac ccttgccatc cagcttctgg
     1681 ccgatataca ggccagcgcg tcgagcggcg gcgacgttgt ccgtgcgcac gacagaaacg
     1741 acttgacccg ctgtgggcgc tgtatctatc gcgatcacag gaatccccgc gacgttggcc
     1801 gatgcgatcc cgggcgcgat tgcggcagca tcgatcggcc caagcaagat gccatcgact
     1861 tgattcgtga tcgcatcatc gatctgggag agttgctcgg gaacgctcgg cgcgctgagt
     1921 gggacaatgt cgacggtcac cccgtccgcc gttgccctgc cggccacagc attctgcacc
     1981 gaccgccaaa acggatcatc cttgaatgga acgaggagcc gcaagcggta ggatcgggct
     2041 gctcgtaccg gaccagtctc gggagcggag gtatcaagca acttctggac cggaatcggg
     2101 ctggccgctg gagttgcctc ttgggcggcg ctgagcctta tcgagaccgg agtcgttgca
     2161 gcagataccg cgatcgcgat cagccgccgc cggttgattt gaacgctgca ctcaatccgg
     2221 gtcaacgcac
//
LOCUS       DASSGX010000573         2817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1626730, whole genome shotgun sequence.
ACCESSION   DASSGX010000573 DASSGX010000000
VERSION     DASSGX010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1626730"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..713)
                     /gene="ilvN"
                     /locus_tag="VFI12_10420"
     CDS             complement(159..713)
                     /gene="ilvN"
                     /locus_tag="VFI12_10420"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF008864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HET7056868.1"
                     /translation="MADSDTKGVLDLEDLRVSNRLTGRRHLLSLLVQNRPGVLARIAG
                     LFSRRGFNIDTLAVGPTEDPDISRVTLTVDGAVHPIDQVTKQLHKLVNVIKIRDMDPG
                     TAVAREMALFRINAAADNRAEIIEFAQIFRAHIVDVSRRSITVEVTGTTEKIDSFERM
                     VRPHGLIEMARTGEVAIVRARPES"
     gene            complement(735..2414)
                     /gene="ilvB"
                     /locus_tag="VFI12_10425"
     CDS             complement(735..2414)
                     /gene="ilvB"
                     /locus_tag="VFI12_10425"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HET7056869.1"
                     /translation="MRAADALCEALKAEGVKHVFGIPGGASLPLYDALYDADFEHVQV
                     RHEQCAGHAAEGYAKASGNVGVAFATSGPGATNLVTAIADAHMDSVPTVFITGQVRTD
                     LIGTDGFQEADVTGITLPIVKHSFLVTDAAQIPDYIHKAFHIAKTGRPGPVLVDIPQD
                     LSKVEIDYEPIVSTPKLPGYQPNTEGNIKQIRIAAKALANSKRPVLYAGGGVINGGAS
                     EELRELAASDELPVTSTLMGLGGFPAPHPQWLGMLGMHGTRTANYAMDEADLIVAIGA
                     RFDDRITGKLSEFAPAAKFVHIDVDPAEISKNVPAHIPIVGDVKNVLPKLTNEYRALG
                     ADGSRLDDWWERIRGWQEQYPLHYEDSTDQEIKPQFMVEEMYRATGGDAIITSDVGQH
                     QMWAAQYYHFDKPNRWLNSGGLGTMGFGLPAAIGAQVAMPDQQVVCLAGDGSLIMVCQ
                     EFATAAAGNLPVKVFLMNNGHFGMVRQWQELFWDERYKSVEMGPSPDWVKLAEAFGWT
                     GMLCDRKDDLPAAMKSALETDGPVLLDVRVTQNENCYPMIPAGAAARDMVG"
     gene            complement(2544..>2817)
                     /locus_tag="VFI12_10430"
     CDS             complement(2544..>2817)
                     /locus_tag="VFI12_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056870.1"
                     /translation="CFAIATGAIGRAKGSSFFLWLVIGGVLPVLGLIGALLYRREHGE
                     PERRCPRCGTTHKLYVQVCNKCGEDMYLPDPSEVRPGPDFRASQSG"
BASE COUNT          449 a          920 c          963 g          485 t
ORIGIN      
        1 gcgatcgtcc gcgcggcgag acgcgaaggc gatcgcacag gggtcgatcg cgtcgacctc
       61 ggcggtgacg ctgacgagac gacgtccgga accgagcgat gcgcgttccg cgagcgaccc
      121 gatcgagtcg agaagcccgg cggtatcgat cgaggcggtc agctctccgg gcgcgcccgg
      181 acgatcgcga cctcaccggt gcgagccatc tcgatcaggc cgtgcgggcg gaccatccgc
      241 tcgaaggaat cgatcttctc ggtcgtcccg gtgacctcga cggtgatcga acggcgcgag
      301 acgtcgacga tgtgagcgcg gaagatctgg gcgaactcga tgatctcggc ccggttgtcg
      361 gccgcggcgt tgatccggaa cagcgccatc tcacgggcga cggcggtgcc ggggtccatg
      421 tccctgatct tgatcacgtt gacgagcttg tgcagctgct tggtgacctg atcgatcggg
      481 tggacggcgc cgtcgacggt gagcgtgacc cgggagatgt ccggatcctc ggtcgggccg
      541 accgcgaggg tgtcgatgtt gaagccgcgg cgactgaaca ggccggcgat ccgtgccagg
      601 acgccgggcc ggttctggac cagcagcgag agcaggtgac gccggccggt gagtcggttg
      661 gagacgcgca ggtcctcgag gtcgagcacg cccttggtat ctgaatcagc catgagggtc
      721 ctgactccct tcgcctaacc gaccatgtcc cgggcggcgg cgcccgccgg gatcatcggg
      781 tagcagttct cgttctgggt cacgcgcaca tcgagcagga ccgggccgtc ggtctccagc
      841 gcggacttca tcgctgcggg gagatcgtcc ttgcgatcgc agagcatccc ggtccagccg
      901 aacgcctcgg cgagcttcac ccagtcgggg ctcggcccca tctcgaccga tttgtagcgc
      961 tcgtcccaga acagctcctg ccactggcgg accatgccga agtgaccgtt gttcatcagg
     1021 aagaccttga ccgggaggtt gccggcggca gcggtggcga actcctggca aaccatgatc
     1081 agactgccgt cgccggccag gcagacgacc tgctgatccg gcattgcgac ctgagctccg
     1141 atcgccgccg ggaggccgaa gcccatcgtc cccagcccgc cggagttgag ccaccggttc
     1201 ggcttgtcga agtggtagta ctgcgccgcc cacatctgat gctggccgac gtcggacgtg
     1261 atgatcgcgt cgcccccggt ggcccggtac atctcctcga ccatgaattg aggcttgatc
     1321 tcctgatcgg tcgagtcctc gtagtgcagc ggatactgct cctgccaacc gcggatccgc
     1381 tcccaccaat cgtcgaggcg ggaaccgtct gcgccgagcg cccggtactc gttggtcagc
     1441 ttcggcagca cgttcttgac gtccccgacg atcgggatgt gggcggggac gttcttggag
     1501 atctccgccg gatcgacatc gatgtggacg aacttcgcgg ccggtgcgaa ctcgctcagc
     1561 ttgcccgtga tccggtcgtc gaagcgcgcc ccaatcgcga cgatcaggtc ggcctcgtcc
     1621 atcgcgtagt tggccgtcct ggtcccgtgc atgccgagca tcccaagcca ctgcggatgc
     1681 ggggccggga agccgccgag gcccatcagc gtgctggtga cggggagctc gtcggaggcc
     1741 gccagctcgc gcagctcctc ggacgcgccg ccgttgatca caccgccgcc cgcgtagaga
     1801 acgggccgct tcgagttggc cagtgccttc gcggcgattc ggatctgctt gatgttgccc
     1861 tcggtgttcg gctggtagcc gggcagcttc ggcgtgctga cgatcggctc gtagtcgatc
     1921 tccaccttcg agagatcctg cgggatgtcg acgaggaccg ggccgggccg gccggtcttc
     1981 gcgatatgga aggccttgtg gatgtagtcg gggatctgcg cggcgtcggt gaccaggaac
     2041 gagtgcttga cgatcggcag cgtgatgccg gtgacgtcgg cctcctggaa cccgtccgtg
     2101 ccgatcaggt cggtgcggac ctggccagtg atgaagacgg tcggcaccga gtccatgtgg
     2161 gcatcggcga tcgcggtgac caggttggtc gcgccgggcc ccgaggtcgc gaaggccacg
     2221 ccgacgttgc cggaggcctt cgcatatccc tccgccgcat ggccggcgca ctgctcgtgg
     2281 cggacctgca catgctcgaa gtcggcatcg tagagcgcgt cgtagagcgg caggctcgcc
     2341 ccgcccggaa tcccgaatac gtgcttgaca ccctcggcct tgagagcctc gcagagtgcg
     2401 tcagcagcgc gcatcgaaat tacctccgga tcgtttgcag gttgtaaggg gcgaatgccg
     2461 cgaccgacct cggctaggtc gactcgcggc tgcatacaag tgtaacagcc gttttcaggg
     2521 tcctgagcgg ccttcggcat ccctcagccc gattgcgagg ccctgaaatc cggccccggc
     2581 cggacctctg aagggtcggg caggtacatg tcctcaccac atttgttgca gacctgcacg
     2641 tagagcttgt gggtcgtccc gcagcgcgga cagcggcgct cgggctcgcc gtgctcgcgc
     2701 cggtagagaa gcgcaccgat cagccccaga accggcagga cgcccccgat cacgagccag
     2761 aggaagaagc tcgacccctt cgcccggccg atcgccccgg tcgcgatcgc gaagcag
//
LOCUS       DASSGX010000574         2010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1408613, whole genome shotgun sequence.
ACCESSION   DASSGX010000574 DASSGX010000000
VERSION     DASSGX010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408613"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="VFI12_10435"
     CDS             <1..471
                     /locus_tag="VFI12_10435"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HET7056871.1"
                     /translation="NNGFYPVYYGDGIGWVAGKYLSWEPVGGGQERWIDVDRSSSTIR
                     LMVGDESIGYWWAAMGFDPSDDGFYSTANGTYYVYEKNAALTWTDWAKGYIEYWVAFD
                     PDRFNGFHSWTMDENGTVIEGGDGATGGCVAMEPGLAAALYEFATFGMRVEIHW"
     gene            607..>2010
                     /locus_tag="VFI12_10440"
     CDS             607..>2010
                     /locus_tag="VFI12_10440"
                     /inference="COORDINATES: protein motif:HMM:NF021489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HET7056872.1"
                     /translation="MQRWSLTSAWRVPAILILAAMMVVVGLDLASPRGAEARSVTWKS
                     YDVTLTLNGDGTFHVSERQVVDFTGGPFTFAFADIPLTRVEDITNITVSERRGNNSVP
                     YDRVGTKDSETVTVQRSSAAMHVTWYMPPTSNQERTFVLEYDVIGGLRVYDSPSGQRQ
                     QIWWTAIDSEVTDTAPIDAATFTIQLPQAVDTANVVLDGPGSTKPEDHTTDGRVYTWS
                     EQNMSGGDSLTARLEFPALVNATAPAWQQADDEQRIREQDRDSRNALLKLLLAGAGLL
                     SLTAGGVGLLGLWYTRGRDPGVGAVASYLSEPPDDLPPGAAGALVDETVDERDVVATL
                     VDLGRRGVLNISAKEAGTVFKRTEYTIELKKDTGELRPFEKTFVDAIMGGTDPGRTVS
                     LGDAKERFARKVNSIKDQMYDELVKRGYFPVSPQKTRSRYQGVASTALIGGFIILVIF
                     GSSILGVSGWFVVPIIAI"
BASE COUNT          371 a          608 c          674 g          357 t
ORIGIN      
        1 aacaacggct tctatccggt ctactacggc gacggcattg gctgggtggc cggcaagtac
       61 ctgagctggg agccggtggg cggtggccag gagcgctgga tcgatgtcga tcgcagcagc
      121 tcgacgatcc gcctgatggt cggcgacgag tcgatcggtt actggtgggc cgcgatgggc
      181 ttcgacccat ctgacgacgg cttctattcg acagcgaacg gcacctacta cgtctacgag
      241 aagaacgccg cgctgacctg gaccgactgg gccaaggggt acatcgagta ctgggtcgca
      301 ttcgacccgg atcgtttcaa tggctttcac tcctggacca tggatgagaa tggcaccgtg
      361 atcgaagggg gagatggcgc aaccggcggc tgcgtcgcga tggagccggg cctcgcggcc
      421 gcgctctacg aattcgcaac cttcgggatg cgagtcgaga tccactggta aagcgcacgc
      481 tcacatctgc gacagcgtat ggagaccccg gccttgccgg ggtctcttgc gttcatgcca
      541 aggcccttgt taccgggcat gggataatgc cggagcgatt cgatatcgat ggggtaggtc
      601 gcttcaatgc aacggtggtc cctgacgtcc gcgtggcgtg tgcccgcaat cctgatcctg
      661 gccgcgatga tggtcgtcgt cgggctggat ctcgcgtcgc cgcgtggtgc cgaggcgagg
      721 tcagtaacgt ggaagagcta cgatgttacg ctcaccctga atggcgacgg cacatttcac
      781 gtctctgaac ggcaggtagt cgacttcacg ggcgggccgt tcaccttcgc cttcgcggat
      841 atcccgctca cgcgcgtcga ggacatcaca aacattactg tcagcgagcg gcgtggcaac
      901 aactcggtgc catatgatcg tgtgggcacc aaggacagcg agacagtgac ggtccagcgc
      961 agcagcgccg cgatgcacgt tacctggtac atgccgccga catccaatca ggaacgcacc
     1021 tttgtgctgg agtacgacgt catcggcgga ctccgcgtct acgactcacc ctccggccaa
     1081 cgccagcaga tctggtggac ggcgatcgac agcgaggtga ccgacaccgc gccgatcgac
     1141 gcggcgacct tcaccatcca gctcccgcag gcggtcgata cggccaatgt tgtgctggac
     1201 gggccgggct caaccaagcc cgaggaccac acgaccgacg gtcgggtcta cacctggtcc
     1261 gagcagaaca tgagcggagg cgactcgctg acggcccggc tcgagttccc cgcgctggtc
     1321 aacgcgaccg cgccggcgtg gcagcaggcc gatgacgagc agcggatccg cgagcaggat
     1381 cgggacagca ggaatgcgct cctgaagctg cttctggccg gcgccgggtt gctgagcctg
     1441 acggcaggcg gcgtgggatt gcttggcctc tggtacacgc ggggccggga tcccggcgtc
     1501 ggcgccgtcg cgtcctacct cagcgagccg ccggatgacc ttccgcccgg cgcggccggc
     1561 gcactcgttg atgagacggt ggatgagcgc gatgtcgtcg cgacactggt cgatcttggc
     1621 cggcggggtg tcctgaacat ctcggccaag gaggcgggaa ccgtcttcaa gcggaccgaa
     1681 tacacgatcg agttgaagaa ggacacgggc gagcttcggc cgttcgagaa gacgttcgtc
     1741 gacgcgatta tgggcggtac cgatccagga aggacggtca gccttggcga tgcgaaggaa
     1801 cgctttgccc ggaaggtcaa ttcgatcaag gaccagatgt atgacgagct ggtcaagcgc
     1861 gggtacttcc cggtctcgcc acagaagacc cgctcgcggt atcagggcgt cgcatcgacc
     1921 gcgctgatcg gcggcttcat catcctggtg atcttcggca gctcaatcct cggggtcagc
     1981 ggctggttcg tggtcccgat catcgcgatt
//
LOCUS       DASSGX010000575         2529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1518545, whole genome shotgun sequence.
ACCESSION   DASSGX010000575 DASSGX010000000
VERSION     DASSGX010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1518545"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="VFI12_10445"
     CDS             complement(<1..782)
                     /locus_tag="VFI12_10445"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HET7056873.1"
                     /translation="MIATVSASRSAAVIGGAFIISRLLGILRDILLGHRFGVSAELDA
                     YYAAFRIPDLVFLGVMSVAFGAAFIPVFGGFLAKGDEEAAWKLSSAVIELAMIATVLL
                     CVVAFALADPLMRHVIAPDLPAEVMPEAIRTMRILLLSPLLIGLGIAAKGILEAQDLF
                     TLPALAPVAYNAAIIFGIVALAPSLGIEGVAIGAVIGAAAHIFVQAPGVLRSGFRFLP
                     ATRPLGVAGVSEVVRLLGPRVIGQAAFQINFIWITGLANRSG"
     gene            981..1253
                     /gene="rpsT"
                     /locus_tag="VFI12_10450"
     CDS             981..1253
                     /gene="rpsT"
                     /locus_tag="VFI12_10450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HET7056874.1"
                     /translation="MANNKQSKKRILINETKRLRNRPYRSAARTYVKKAEVAIRSGDQ
                     NAAAAAVGDALSMLDRVASKGIIHQNNAARRKSRLMKKYNGIGAEA"
     gene            complement(1309..2304)
                     /locus_tag="VFI12_10455"
     CDS             complement(1309..2304)
                     /locus_tag="VFI12_10455"
                     /inference="COORDINATES: protein motif:HMM:NF017911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056875.1"
                     /translation="MIYLLVGPDRYLLERELKRILAEVDPDGLNTTRYDKSASISEVG
                     NAVATAGFFGTGRVIVAEGVMQRAAGTGKAKKAEAEEFDALFTSVAPGNTLILVDPEI
                     GTVPTAVKKAAGPDATQFGGRVPRGHDLVEWAQRQVTEMGGSIEPRNVNAILDRLYPG
                     TWREANRNPAFDVPPDLLLLSNELAKLVAAADGKEINARHVKELVPLATGEDLFPFID
                     AVVGGNAAGAFKLLSGDQTDDDTAARYLNQLGSRAERGQALAASGAQDVADVGKRVGA
                     TNPSSIQKTFARTSAESFANVVLESDRRLKTGKTRSPSEQLQDVIVRQARKTRGH"
     gene            complement(2330..2460)
                     /gene="ssrS"
                     /locus_tag="VFI12_10460"
     ncRNA           complement(2330..2460)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VFI12_10460"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
BASE COUNT          458 a          785 c          866 g          420 t
ORIGIN      
        1 tcgccggagc gattggcaag ccctgtgatc cagatgaagt tgatctggaa cgccgcctgc
       61 ccgatcacgc gaggccccag cagccggacc acctctgaga cgccggccac gccaagcggg
      121 cgagtggccg gcaagaaccg aaaccccgag cggagaactc ccggcgcctg gacgaagatg
      181 tgcgccgccg cgccgatcac cgcgccgatc gcgaccccct caatgcccag cgatggggcg
      241 agcgcgacga tgccgaagat gatcgccgcg ttgtaggcga ccggagcgag cgccgggagg
      301 gtgaacaggt cctgcgcctc gaggatgccc ttcgcggcga ttcccaggcc gatcagcagc
      361 ggagagagga gcaggatacg cattgttcgg atcgcttccg gcatgacttc agccggcaag
      421 tcgggcgcga tcacgtgccg catcagcggg tcggcaaggg cgaacgcgac gacgcagagc
      481 aggaccgtcg cgatcatcgc cagctcgatc acggccgacg agagcttcca cgcggcttct
      541 tcgtcccctt tggcaaggaa gccgccgaag accgggatga aggcggctcc gaaggcgacc
      601 gacatgaccc caaggaagac gagatccggg atgcggaatg ccgcgtagta ggcgtcgagc
      661 tccgcgctga cgccgaagcg gtggccgagc aggatgtcgc gcaggatgcc gagcaaccgg
      721 ctgatgataa atgccccgcc gatgaccgcc gcgctgcgcg aggcagaaac cgtcgcgatc
      781 attggtccgg ggggcggctc ggactcgaat gagacgatgg gcggggtggt gcggggctgg
      841 agatatggcc ggacgccgaa cggacggttg agtccgtcgc cggcatttgg tattctttgc
      901 cggctagggc tggccgtcga cggccggttg ccctgtgctg tcacgcgatc tcagccccgt
      961 ctttcgaaag gtaacggacc ttggccaata acaagcaatc gaagaagcgg atcctgatca
     1021 acgaaacgaa gcggctccgc aatcgccctt atcgctctgc tgcccgcacc tacgtcaaga
     1081 aggcggaagt tgcgatccgg agcggtgacc aaaacgccgc cgccgccgct gtgggcgatg
     1141 ccttgagcat gctcgatcgc gtcgcgagca agggtatcat ccaccagaac aatgctgcac
     1201 ggcggaagtc ccggttgatg aagaagtaca acgggatcgg cgccgaagcg taggacggag
     1261 tcgtcagact tcggacatca gacgtcggaa cctgggagcg cgcggtcgtt agtggccgcg
     1321 cgttttgcgt gcctggcgga cgatgacgtc ctggagctgc tccgatgggg agcgggtctt
     1381 gccggtcttg aggcggcgat cggactccag gaccacattc gcgaatgact cggcgctagt
     1441 ccgggcgaag gtcttctgga tggaggatgg attggtcgcg ccgacccgct tgccgacatc
     1501 cgcgacatcc tgtgctccag acgcggccag ggcctgacct cgttccgcgc gcgagccgag
     1561 ctgattcagg tagcgagctg cggtgtcatc gtccgtttga tcacccgaga gtagcttgaa
     1621 cgcgccggcc gcattgccgc cgacaacggc gtcgatgaac gggaagaggt cctcgccggt
     1681 cgcgagcggg acgagctcct taacatggcg ggcgttgatt tccttgccat ccgccgccgc
     1741 gacgagcttt gccagctcgt tggacagcag caggagatcg ggcggcacgt cgaaggccgg
     1801 attccggttc gcttcccgcc atgtgccggg gtagagacga tccaggatgg cgttgacgtt
     1861 gcgcggctcg atgctcccgc ccatttcggt cacttgccgt tgcgcccact cgacgaggtc
     1921 gtgcccgcgc gggacccgac cgccgaattg ggtcgcatcg ggaccggcgg ccttcttgac
     1981 ggcggtggga acagtgccga tctcggggtc gaccaggatt agcgtgttgc ccggcgcgac
     2041 ggacgtgaag agggcgtcga actcctctgc ctcggccttt ttcgccttgc cggtccccgc
     2101 cgcgcgctgc atcacccctt cggccacgat cacgcggccg gttccgaaga agccggcggt
     2161 cgcaaccgcg ttgccgacct cgctgatgga ggcggatttg tcatagcgtg tggtgttcag
     2221 gccgtcggga tcgacctcgg cgaggatgcg tttcagctcc cgctcgagca gatagcgatc
     2281 gggtccgacg aggagataga tcatggatgc gtttgtccgg tgaggagtca gtgaacccgc
     2341 gagtgccgtg tgtctacgga gccttgaacc tgctctcgca ggtgggtacc gcttacgcag
     2401 ccccgactca tttctatcgg ctcaagcccg cgcagagcat cacgggcacc gcaggctcac
     2461 cgtttgctga gcctagcacg ggccagaacg acagggcaac cgattccgcg tggtagcatc
     2521 accggcttg
//
LOCUS       DASSGX010000576         3092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_865371, whole genome shotgun sequence.
ACCESSION   DASSGX010000576 DASSGX010000000
VERSION     DASSGX010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865371"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1306
                     /locus_tag="VFI12_10465"
     CDS             <1..1306
                     /locus_tag="VFI12_10465"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF006004.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HET7056876.1"
                     /translation="DHVFGFNTLAVHAGQRPDPTTGARAMPIYQTTSFVFEDTDHAAE
                     LFALQRFGNIYSRIMNPTNGAFEERMATLEGGVGALSVASGHAAQFIALATILSPGDQ
                     LVASNALYGGSYTQLGITLRKWGIDVVFVDPDDPENFRRAITPKTRALYGETIGNPRI
                     NVLDIEALANIAHEAGVPLIIDNTFATPYLCRPLDWGADIVVHSATKFIGGHGTSIGG
                     VIVDAGRFPWDNGKFPGMTEPSPGYHGMRFWEQFSIYAYIMKARVEALRDFGPALSPF
                     NAFLFLQGLETLHVRMDRHVQNAEKVAQYLADHPLVSWVNYPGLAGDPYHALAQKYMP
                     KGAGAIFCFGVKGGRETGKAFIESLDMISHLANVGDAKTLVIHPASTTHQQMSDEEQI
                     AAGLSPDLIRISIGLEDPADINWDLNQALTRAARTASVSAGA"
     gene            1310..1804
                     /locus_tag="VFI12_10470"
     CDS             1310..1804
                     /locus_tag="VFI12_10470"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HET7056877.1"
                     /translation="MTIATDHVPSASSSERLRILNEHKTIAMVGLSSDPMRPSHFAAI
                     YMQAEGYKIVPVNPRYAGQKILGETVYASLSDIPFKVDIVDVFRRPEECPDLAREAIK
                     IGAKVFWIQLGLECDEAGKIARDGGLDFVQNRCVKIEHARFHGGMNLVGLNTGVISGR
                     RALR"
     gene            1801..2217
                     /locus_tag="VFI12_10475"
     CDS             1801..2217
                     /locus_tag="VFI12_10475"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HET7056878.1"
                     /translation="MIEPTDDEIRSLLQRARRIAVVGLSSDVTRPSYGVSAYLQHAGY
                     EILPVNPKHAGETILGQPVVAKVTDLEGPIDIVNIFRRPIDVPRPVDEAIAAGAGAVW
                     MQLGIRNDDAGQRAADAGLFVVMDRCISVAHRLLVG"
     gene            complement(2509..3057)
                     /locus_tag="VFI12_10480"
     CDS             complement(2509..3057)
                     /locus_tag="VFI12_10480"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET7056879.1"
                     /translation="MATRRRTRDEMPAEHALLGLLAQSTGSRHGYDLAREFQSGSELG
                     EVIRLEPGMLYHHLKKMERAGWVTSHQEVVERRPARSVYALTLAGEAELDRWMSEPVG
                     HTREIRLAFLVKLYLARQTDSALAERLIREQIATLGEIETTHLTDANGGDFSAAVRAL
                     RLAQTRTALAWLRTLLTESQSR"
BASE COUNT          545 a         1058 c          990 g          499 t
ORIGIN      
        1 cgaccacgtc tttgggttca acacgctcgc cgttcacgcc gggcagcggc cggacccgac
       61 caccggcgcg cgggcgatgc cgatctacca gaccacgagc ttcgtcttcg aggacacaga
      121 ccacgcggcc gagctcttcg cgctccagcg gttcggcaac atctactccc ggatcatgaa
      181 cccgacgaac ggcgccttcg aggagcggat ggccacgctc gaaggcggtg tgggcgcgct
      241 ctcggtcgcc tccggccacg cggcccaatt catcgcgctg gccacgatcc tgagccccgg
      301 cgatcaactg gtcgcctcga acgcactcta cggcggcagc tacacgcaac tcggcatcac
      361 gctccggaag tggggcatcg acgtcgtctt tgtcgacccg gacgatccgg agaacttccg
      421 ccgggcaatc acgccgaaaa cgcgcgccct gtatggcgag acgatcggca acccgcgcat
      481 caacgtgctc gacatcgagg cgctggcgaa catcgcccac gaggcgggcg taccccttat
      541 catcgacaac accttcgcca cgccctatct ctgccggccg cttgattggg gcgccgatat
      601 cgtcgtccac tccgccacca agttcatcgg cgggcacggc acgtcgatcg gcggcgtgat
      661 cgtcgatgcc ggccgcttcc catgggacaa cggcaagttc ccgggcatga ccgaaccctc
      721 gccgggctat cacggcatgc gcttctggga gcaattcagc atctacgcct acatcatgaa
      781 ggcgcgggtc gaggcactgc gcgactttgg cccagccctg agcccgttca acgccttcct
      841 cttcctgcag ggcctggaga cgctccatgt ccgcatggac cgccatgtcc agaacgcgga
      901 aaaggtagcc caatacctcg cggaccaccc gctcgtgagc tgggtcaatt acccgggcct
      961 ggccggcgac ccgtaccacg cgctggcgca gaagtacatg cccaagggag ccggggcgat
     1021 cttctgcttt ggcgtgaagg gcgggcgcga gaccggcaag gccttcatcg agagcctcga
     1081 catgatctcg cacctcgcca atgtcggcga tgccaagacc ctggtcatcc acccggccag
     1141 cacgacgcac cagcagatga gcgacgagga gcaaatcgcc gccgggctca gcccggacct
     1201 gatccggatc tcgattggcc tcgaggatcc ggccgatatc aactgggacc tcaaccaggc
     1261 gcttacccgg gcggcccgca ccgcctccgt cagcgcggga gcatgagtta tgacaatcgc
     1321 aactgatcat gtcccgtccg catccagctc ggagcgtctg cgcatcctga acgagcacaa
     1381 gacgatcgcg atggtcggcc tctcctccga cccgatgcgc ccgagccact tcgcggcgat
     1441 ctacatgcag gccgagggct acaagatcgt cccggtcaat ccgcggtacg ccgggcagaa
     1501 gattctcggc gagacagtct acgcctcgct gagcgacatc ccgttcaagg tcgatatcgt
     1561 cgatgtcttc cgccggccgg aagagtgccc ggaccttgcc cgggaggcga taaagatcgg
     1621 cgcgaaggtc ttctggatcc agcttgggct cgaatgcgat gaggccggca agatcgcccg
     1681 cgatggcgga ctcgatttcg tccagaaccg ttgcgtcaag atcgagcatg cgcgcttcca
     1741 cggcggcatg aacctggtcg gcctcaatac cggagtgatc agcggccggc gggcgttgcg
     1801 gtgatcgaac cgaccgacga cgagatccgg tcgctgctgc agcgcgcgcg gcgcatcgcg
     1861 gtcgtcgggt tgtcgtccga cgtcacccgg ccgagctacg gcgtctccgc ctatctgcag
     1921 cacgcgggat acgagattct gccggtcaat ccgaagcatg ccggtgagac gatcctcggc
     1981 cagccggttg tggccaaagt gaccgacctt gaggggccga tcgacatcgt caacatcttc
     2041 cgccggccga tcgacgtccc ccgcccggtc gacgaggcaa tcgcggccgg tgccggcgcg
     2101 gtctggatgc agctcggcat ccgcaacgac gatgccggtc aaagggcagc tgacgccggc
     2161 ctcttcgtcg tcatggaccg ctgcatctcc gtcgcacacc gcctcttggt aggttgagag
     2221 ttgagggcgg agggtggaga gagacgacgt aggggcagat ggcatctgcc cgccgctctc
     2281 ccgaatgccc ggtcgcatgg gagcgccggc gccccggatt gcccgggcgt atacacccgg
     2341 gctgacatct gaacccggct gggtagctca tccaaacgta gcgcggggtt accatgcccc
     2401 gcccgggccc ggtgacagcg cgatagttaa cgggaaccga tccggacaac gatcatcgcc
     2461 ctgcgggcga tcgcggggct tccgcaccgc gctacgtcag acggtccatc accgggattg
     2521 gctctcggta aggagcgtgc ggagccaggc gagggcggtg cgggtttggg cgaggcgcag
     2581 cgcacggact gcggccgaga agtcgccgcc attcgcatcg gtgaggtgcg tggtttcgat
     2641 ctccccgagg gtcgcgattt gctcgcgaat cagccgctcg gccagggctg agtcagtctg
     2701 gcgggccagg tagagcttga ccaggaaggc caatcggatc tcccgggtgt ggccgaccgg
     2761 ctcgctcatc cagcgatcga gctccgcctc acccgcgagc gtcagcgcgt agacggaccg
     2821 ggccggacgc cgctcgacga cctcctggtg cgaggtcacc caaccggcgc gctccatctt
     2881 cttgaggtgg tgatagagca tccccggttc gagccggatc acctcgccca gctcggagcc
     2941 ggactggaac tcgcgggcca ggtcgtaccc gtgccggctg ccggtcgatt gcgccaacag
     3001 gccaagcagg gcatgctccg cgggcatctc gtctcgggtt cggcgccgag tggccatcgc
     3061 gatccttccg cgcggaaatc aacactccgg ct
//
LOCUS       DASSGX010000577         3142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_753959, whole genome shotgun sequence.
ACCESSION   DASSGX010000577 DASSGX010000000
VERSION     DASSGX010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753959"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(233..2134)
                     /locus_tag="VFI12_10485"
     CDS             complement(233..2134)
                     /locus_tag="VFI12_10485"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET7056880.1"
                     /translation="MPSNTPVLSSAIRSFADLDLRKTTHNALARVHITSPTPIQAGSL
                     PVLLAGRDLIGQSRTGSGKTLAFGIPAVESVDPAIKAVQVLVLTPTRELAVQVGAVLH
                     SLAEGTGIRSVLVYGGRAMGPQKDALRRGAQIVVGTPGRVADLYGQGDLRLDNVRFLV
                     LDEADEMLDRGFGPQVAKILSWTPRERQTALFSATMPEWVDDAARKHLRNPEHVAIQT
                     TAAEAPAIDHVAYDVPAGGKMDALKDLLDARGDGQVIVFGRTKHGVKKLAKQLQAAGY
                     PVAALQGNMSQNARDAVMADFRAGKIAILLATNVAARGIDVSAVDHVINFELPESSEL
                     LQHRIGRTGRMGRTGSAVTLLTDEDADKWRQLMRGLGKQISRKPWTGVRRTDAAPRVI
                     EGRASEQASRRAAEGRGGAQPRSRNVESRGNATSLGEASSVQPGVSTPGRGGHENGRR
                     NPETRKPRPENRPESNGTRDRGRSRREQQPAAAQGQRREAGISVVSEMIRERRSGPQS
                     TDRRPRAQAPEANGTALRGSFQIEDLIDQEKFAFRNRGVSRHSERKADFGTIAPNGTG
                     RPAFPIREERLQAEAPAGQPEGQHTAICSNCGGTATLRFAPDLSRPIYCDACFSERRK
                     NRRRPSAQA"
     gene            2693..>3142
                     /locus_tag="VFI12_10490"
     CDS             2693..>3142
                     /locus_tag="VFI12_10490"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET7056881.1"
                     /translation="MHVHERITVDRPPAQVYAFWRNLSQLPLFMDHLVSVQGDPDGVT
                     RWTAESPIGTVHWDAKVVEDKPNEMISWRSLEDSGVRNSGAVRFFPAGNGATEVAVDI
                     SYDPPMGRLGEIVASLLGESPHQEISSDLKRFKKIMESGQADVSGSPV"
BASE COUNT          515 a         1003 c         1083 g          541 t
ORIGIN      
        1 attgcacctc atcccagccg gcttcagcgg gcttcaggga ttcagccctg gtgttatcgc
       61 ccgggcgatg ccgggcacga gccctcgtat gcgagcgggc cggtcagaaa cggcgggttg
      121 gcgtcagcaa acccctgcgg atgaccctcg acattcggaa ggttcgacag agatgcccgg
      181 gcttgcctgt gcaaacccgg gcaatttatt gtgctcgaaa tggtgccggg cgctatgcct
      241 gggcggaggg gcgccggcgg ttcttgcggc gctcgctgaa gcaggcgtcg cagtagatcg
      301 ggcggctcaa atccggtgcg aacctgaggg tggccgtccc gccgcagttg gagcagatcg
      361 cggtgtgctg gccttccggt tgcccggcag gagcctctgc ctgcagccgc tcctcgcgga
      421 tcgggaaggc cggacgcccg gtcccgttcg gcgcgatcgt gccgaagtcg gccttgcgct
      481 ccgagtggcg tgagacgccg cggttccgga aggcgaactt ctcctggtcg atcagatcct
      541 cgatctggaa cgatccgcgc agcgccgtgc cattcgcctc tggcgcctgg gcacgcggcc
      601 ggcggtctgt ggattgcgga ccgctccggc gctcccggat catctccgag acaaccgaga
      661 ttccggcctc gcggcgctgg ccctgtgcgg cagccggctg ctgctcgcgc cgggatctcc
      721 cgcggtcccg ggtaccgttg ctctctggtc ggttttcggg tctcggtttt cgggtttcgg
      781 ggtttcggcg tccgttttca tggccgcccc gccccggggt tgaaaccccg ggttgaacgg
      841 acgaagcctc tccgaggctg gtcgcattgc cacgcgactc tacatttcga ctccgcggct
      901 gcgcgcctcc gcgaccctct gcagctcgcc tgctcgcctg ctcgcttgct cgcccttcaa
      961 tcactcgtgg tgcggcatcc gtccggcgaa cgccggtcca gggcttgcgc gagatctgct
     1021 tgccgaggcc gcgcatcagc tgccgccact tgtcggcgtc ctcgtcagtc agcaacgtga
     1081 ccgccgagcc ggtgcggccc atgcggccgg tacggccgat gcggtgctga agcaactcgg
     1141 acgactccgg cagctcgaaa ttgatgacgt gatcgaccgc cgagacatcg ataccccgcg
     1201 cggcgacgtt cgtggcaagc aggatcgcga tcttgccggc ccggaaatcg gccatgacgg
     1261 catcgcgcgc gttttgggac atgttgccct ggagcgcggc caccgggtag ccggccgcct
     1321 ggagctgctt ggccagcttt ttgacgccgt gcttggtccg gccgaagacg atcacctggc
     1381 catcgccacg ggcatcgagc aggtccttga gtgcgtccat cttgccaccg gccgggacgt
     1441 cataggcgac gtggtcgatc gcgggggcct cggccgccgt ggtctggatc gcgacgtgct
     1501 ccgggttgcg aaggtgcttg cgcgccgcgt catcgaccca ctctggcatc gtggcggaga
     1561 agagggcggt ctgccgttcg cgcggggtcc aggacaggat cttggccacc tgcgggccga
     1621 agccgcgatc gagcatctcg tcggcctcgt cgaggacgag gaagcggacg ttgtcgaggc
     1681 gcaggtcgcc ctggccgtag agatcggcaa cgcggccggg tgtgccgacg acgatttgag
     1741 caccgcgccg gagcgcatcc ttctgcgggc ccatcgcgcg gccgccataa acgagcacgg
     1801 accggatgcc ggtgccctcg gccagggagt gaagcacggc gccgacctgg acggccagtt
     1861 cgcgggtcgg agtcaggacg aggacctgga cggccttgat ggccggatcg accgattcga
     1921 ccgccgggat gccgaaggcg agcgtcttgc ccgagcccgt gcgggactgg ccgatcaggt
     1981 cgcgcccggc gagcaggacc ggcaagctgc cggcctggat cggagttggg ctggtgatat
     2041 gtacgcgagc cagcgcgttg tgcgtcgtct tgcgcaggtc gagatcggcg aatgaacgga
     2101 tcgccgaaga cagaacgggt gtattggaag gcacaaaaaa gctcctgggt acgcgtgcag
     2161 aaaacggcca atgcggccaa cagtgtcaga tcacggggaa ccgactggaa gcggatcagg
     2221 ccgcccagtc atccccctgc tgcacgctgc acaggtacgg gagctgagcg tagcgcacac
     2281 gggcgtatcc cgtgcgatcg cattcgtaat agatagtatg acataacggg agctctgagt
     2341 cgaggaggcg aggaggcgag gaggcgcgaa ggcgcggagt tgagggtgcg gacgtgcagt
     2401 ggtgaggggg tgaacagggg gcgcggaggt ggggtgcagg tgtttgtgga ggcaggccag
     2461 tgctgccgga gcggttgact cctggtgaga gccattacgc tccgcaaccc aaaacccaaa
     2521 acctaaaacc caaaacccaa aacccaaaac ccaaaaaccc gccgtgtagc ccaaacatcg
     2581 atcgtcggta gtgagggggg acgctcctgt tctgtgcccg gggtagggtt ggatcggccg
     2641 ggtgcgcgtg cgcgatttcg gcccatccac tgcctcaagg aggaagcccc tcatgcacgt
     2701 ccacgaacga atcaccgtcg atcgcccgcc cgctcaggtc tacgccttct ggcggaactt
     2761 gtcccaactc ccgctcttca tggaccacct cgtctcggtc cagggcgatc cggacggcgt
     2821 tacccggtgg accgccgaat cgccgatcgg caccgtccat tgggatgcca aggtcgtcga
     2881 ggacaagccg aacgagatga tctcatggcg ctccctcgag gactctggcg tccgcaactc
     2941 aggcgcggtc cgcttcttcc cggccggcaa cggcgcaacg gaggttgcgg tcgatatctc
     3001 ctacgacccg ccgatgggcc gcctgggcga gatcgtcgcc tcgctccttg gcgagtcgcc
     3061 gcaccaggag atctcatcag acctgaaacg attcaagaag atcatggaga gcggccaggc
     3121 cgatgtctcc ggctcacctg tc
//
LOCUS       DASSGX010000578         4080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_753967, whole genome shotgun sequence.
ACCESSION   DASSGX010000578 DASSGX010000000
VERSION     DASSGX010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753967"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2018
                     /gene="rph"
                     /locus_tag="VFI12_10495"
     CDS             <1..2018
                     /gene="rph"
                     /locus_tag="VFI12_10495"
                     /EC_number="2.7.9.6"
                     /inference="COORDINATES: protein motif:HMM:NF004877.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rifamycin-inactivating phosphotransferase"
                     /protein_id="HET7056882.1"
                     /translation="FTADPVSGNRKVTSVEAIYGLGEALVSGTAAADVYTVRDGAIVS
                     TSIAAKRRATIASPSGGTQEQVVAPERQDERALTVAQVIRLAALGRRIEAHFGRPQDI
                     EWCLVDGEFQIVQSRPITTLFPVPDRGDDALHVYLSVGHQQMMTDAMKPLGISVWQLT
                     AGRPMFTAGGRLFVDVTAALATSSGRAGLMAMAGKSDPLTGDALRTIIERGDIIPPRQ
                     DDAPGAPPAAVAPPPIETDPAIVTGLIERDQASIAAAKRDIRAKSGSELLDFIVADLQ
                     ELKRILFDPLSHQAIMAGIEAAWWLNEQLAAWLGEKNAADTLTQSVPGNVTSEMGLAL
                     LNVADAIRPYPAVVAFLEQVRGEDFLDELPKVEGGREGRDAILGWLEKYGMRCAGEID
                     ITRPRWSERPTSLVPLVLGNIRNFAPGEGRRRFERGRREALTREQELLERLRELPDGA
                     QKAAETRQMIDRVRTFAGYREYPKYGMVSRYFVYKQALLAEAARLVQADVIHEPEDIF
                     FLTFQELRDVVRTGQADDQLIRRRKDEFRSYQELTPPRVLTSEGEVITGSYRRDGVPA
                     GALIGLPVSSGTIEGRARVLLDMAKSDLDPGDILVTAYTDPSWTPLFVTIAGLVTEVG
                     GLMTHGAVIAREYGLPAVVGVQDATRLIRDGQRIRVHGTDGYIELLA"
     gene            complement(2064..3059)
                     /locus_tag="VFI12_10500"
     CDS             complement(2064..3059)
                     /locus_tag="VFI12_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056883.1"
                     /translation="MRVSRRSVLTAALGTAAGVAARPAWAQVDPRQLPGKIAFVRNGS
                     VWIWEGGNASELITADNISDPRWSPDGGRLIYVRMQNSFSDLYLFDLTNGVETQLTYN
                     QPYDEVGSEAYAANSSWVLDPDWSRAGLIGFMSDAYGSGGFLSLWLLASPGDNPYLAL
                     QTVAEDDISGLALAPHDPIAAYTVRVWMGNGTFSTYVALRDLNTGFTAPVAQSSGDIF
                     DSAISPDASWVAVTIRDEEGVTDIWLVDRATGERRRGTRGENALAPRWSDDGNWFAYL
                     RMQDYGFEIWAGGFSKGRIRDAAKIYDESGIDSQSGLSWWMQPAGGINTIATPSP"
     gene            complement(3062..>4080)
                     /locus_tag="VFI12_10505"
     CDS             complement(3062..>4080)
                     /locus_tag="VFI12_10505"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HET7056884.1"
                     /translation="IQRLLRQIVDAGVTDAILEATSHGLDLHRLDHTRFQIGVVTNIT
                     QEHLEHHKTIEAYWRAKAKLFERVSHVGGDAVVNLDDAGARSVLPYCGGCRRVIRYSL
                     DDPAADLRVEVDESGLTGSCGRLHVSGTTYPFLVPLVGAFNVSNALAALGTAIAVGVE
                     IEPALAALSSIPAIPGRMASIEAGQPFGVIVDYAHTPESLEKVLRLLRTLRPEGRLIA
                     VFGSAGERDAVKRPIQGRVAAELADIAVITNEDPRYEDAEAIIRQIAAGAIAAGAVEG
                     ETVHCVVERRDAVALALDLAQPGDTILLAGKGHERSIIWNGVKHPWDEATVARELLAE
                     RGWA"
BASE COUNT          702 a         1401 c         1317 g          660 t
ORIGIN      
        1 tgttcacggc cgatccggtc tcgggcaacc ggaaggtgac ctccgtcgag gcgatctacg
       61 gcctcggtga ggcgttggtc tccggcacgg ccgccgcgga cgtctacacc gtgcgtgacg
      121 gcgcaatcgt ctccacgtcg atcgcagcca agcgccgggc gaccatcgcc tcgccgtcag
      181 gtggcacgca ggaacaggtg gtcgcgccgg agcgacagga cgagcgggcg ctgacagttg
      241 cgcaggtaat ccgactcgcg gcgctgggcc ggcggatcga ggcgcatttc ggccggccgc
      301 aggacatcga gtggtgcctg gtcgacggcg aattccagat cgtccagagc cgcccgatca
      361 ccacgctctt ccccgtcccc gaccgcggcg acgacgcgct ccatgtctac ctctccgtgg
      421 gccatcaaca gatgatgacc gacgcaatga agccgctggg catctccgtg tggcagttga
      481 ccgccggacg tcccatgttc acggcgggag gacgactgtt cgtcgacgtc acggcggccc
      541 tggccacgtc gtcgggccgc gcgggcctga tggcaatggc cggcaagtcc gatccgctca
      601 ccggagatgc cctgcgtacg atcatcgaac gcggcgacat catcccgccg cgccaggacg
      661 atgctcccgg cgcgccgccg gccgctgtgg cgccaccacc gattgagacc gatccggcca
      721 tcgtcaccgg gttgatcgaa cgcgaccagg cctcaatcgc cgccgcgaag cgcgatatcc
      781 gggccaaatc gggctcagag ctcctcgact tcatcgtggc cgacctccag gagctgaaga
      841 gaatcctgtt cgatccactg agtcatcagg cgatcatggc cgggatcgag gccgcctggt
      901 ggctgaatga gcagcttgcg gcgtggctgg gcgaaaagaa cgcggccgac acgctgacgc
      961 agtcggtccc cggcaatgtc acatcggaga tgggcctcgc tctcctgaac gtcgccgacg
     1021 cgattcgccc gtacccggcg gtagtggcgt tcctggaaca ggtgcgtgga gaggacttcc
     1081 tggacgagct gcccaaggta gaaggcggac gggagggtcg ggacgccatc ctgggctggc
     1141 tcgaaaagta cggcatgcgc tgcgccggtg agatcgacat cacccggccg cgctggagcg
     1201 agcgcccgac ctcgctcgtg cccctcgtcc tcggcaacat ccggaacttc gcgcccggcg
     1261 agggccggcg gcgcttcgaa cgcggacggc gggaggctct gacaagggaa caggagctgc
     1321 tggagcgcct tcgggaactg ccggacggag cacagaaggc ggccgagacc aggcagatga
     1381 tcgaccgggt ccggaccttc gccggttacc gcgaataccc gaaatacggc atggtcagtc
     1441 gctacttcgt ctataagcag gccctgttgg cggaggccgc tcgccttgtt caagccgacg
     1501 tgatccatga gccagaggac atcttcttcc tcacgttcca ggagctgcgg gacgtcgtgc
     1561 ggacgggaca agcggatgat caactcatcc gacggcgcaa ggacgagttc cgttcatatc
     1621 aggagctcac cccgccccgg gtgctgacgt cggaaggtga ggtcatcacc gggtcgtatc
     1681 gccgggacgg tgtgccggcc ggcgcactga tcggccttcc agtctcatcc gggaccatcg
     1741 aggggcgggc tcgcgtgctc ctcgacatgg ccaagagcga tctcgatccg ggcgacatcc
     1801 tggtcaccgc ctacaccgac ccgagctgga ccccactctt cgtcacgatc gcgggcctgg
     1861 tgacagaggt aggcggcttg atgacccacg gcgcggtgat cgcccgggaa tacggcctgc
     1921 cggccgtcgt cggagtgcag gacgccaccc ggctgatccg ggatgggcag cggatccgcg
     1981 tgcacggaac agacggctac atcgagcttc tggcctgagg agcgcaattg aaaccccggt
     2041 tggcttcgcc cggcagcggg caatcaggga ctcggcgtgg cgatggtgtt gatgccgccg
     2101 gcgggttgca tccaccagct caggccgctc tgcgagtcga tgccggattc gtcatagatc
     2161 ttggcggcgt cccggattcg gcctttggag aaaccgccgg cccagatctc gaacccgtaa
     2221 tcctgcatcc tcaagtaagc gaaccagttg ccgtcatccg accagcgcgg cgccagagcg
     2281 ttctctcccc tggtcccgcg ccgccgctcg ccggtcgccc ggtccaccag ccagatatcg
     2341 gtcacgccct cttcgtcccg gatcgtaacc gcgacccacg atgcatcggg actgatcgcc
     2401 gagtcgaaga tgtcgccgct cgactgcgcg accggggcgg taaaaccggt gttcaggtca
     2461 cgcagggcca cgtaggtgct gaatgtgccg ttgcccatcc acacccggac cgtatacgcg
     2521 gcgatcggat cgtgcggcgc aagcgcgagg ccggaaatat catcctcggc gaccgtctgg
     2581 agcgccagat acggattgtc cccgggcgag gccagcaacc agagcgagag gaacccgccc
     2641 gagccgtatg cgtccgacat gaacccaatg aggccggcac gcgaccaatc agggtcgagc
     2701 acccatgagc tgttcgccgc gtatgcctcg ctcccgacct cgtcgtaggg ctggttgtag
     2761 gtgagctgcg tttcgacccc gttcgtcagg tcgaacaggt aaagatcgga gaagctgttc
     2821 tgcatccgca cgtagatcag gcgaccgccg tccgggctcc agcgcgggtc gctgatgttg
     2881 tcggcagtga tcaactcact cgcgttgccg ccttcccaga tccagacgct gccgttgcgc
     2941 acgaatgcga tcttgcccgg caattgccgc gggtccacct gcgcccacgc agggcgagcc
     3001 gcgacaccgg cggccgtgcc cagcgcggcg gtcaggacgg atcgccggga cacgcgcatt
     3061 gctatgccca tccccgctcg gccagtagct cgcgggcaac cgtcgcctcg tcccacgggt
     3121 gcttcacacc gttccagatg atgctccgct cgtgcccctt gccggccagc aggatcgtgt
     3181 cgccgggttg agcgaggtcc aatgcgagcg cgaccgcgtc ccgccgctcg acgacgcagt
     3241 gcaccgtctc gccctcgacc gcaccggccg caatcgcgcc ggccgcgatc tgccggatga
     3301 tcgcctcagc atcctcatag cgcgggtcct cgttcgtgat cacggcgatg tccgccagct
     3361 ccgccgcgac ccggccctga atcggccgct tcaccgcgtc ccgttccccc gcgctgccga
     3421 agacggcgat cagccgtccc tccggccgca atgtccgcag caacctcagc accttctcca
     3481 gcgattcggg cgtgtgcgcg taatcgacga tcacgccgaa cggctgccca gcttcgatcg
     3541 atgccatccg ccccggaatg gccgggatcg acgacaaggc cgcaagggcc ggctcgatct
     3601 cgacgccaac ggcaatcgcc gttccaagcg ctgcgagggc gttggacacg ttgaaggcgc
     3661 cgaccagcgg caccaagaag ggatatgtcg ttccggatac gtgcaaccgg ccacaactgc
     3721 cggttagccc actctcatcg acctccaccc gcaaatcggc ggccggatca tccagcgagt
     3781 accggattac gcgccggcat ccgccgcagt acggcaagac cgatcgcgcc ccggcgtcgt
     3841 ccaggttcac gaccgcgtcg ccgccgacgt ggctgacgcg ctcgaacagc ttggctttcg
     3901 cccgccagta ggcctcgatc gtcttgtgat gctcgagatg ctcctgggtg atgttcgtta
     3961 cgacgccgat ctgaaacctc gtgtgatcga gccgatgcag atcgagcccg tgcgaggtcg
     4021 cctcgaggat cgcgtcggtt acgccggcat cgactatctg ccgcagcagc cgctgaatat
//
LOCUS       DASSGX010000579         2655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_865441, whole genome shotgun sequence.
ACCESSION   DASSGX010000579 DASSGX010000000
VERSION     DASSGX010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865441"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..259)
                     /locus_tag="VFI12_10510"
     CDS             complement(68..259)
                     /locus_tag="VFI12_10510"
                     /inference="COORDINATES: protein motif:HMM:NF016828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HET7056885.1"
                     /translation="MEAYVSFLNRVIREEEGQDMVEYALLVGLISVAAIAALLLARDA
                     INALWDAVTAALDSATAVL"
     gene            complement(696..1862)
                     /locus_tag="VFI12_10515"
     CDS             complement(696..1862)
                     /locus_tag="VFI12_10515"
                     /inference="COORDINATES: protein motif:HMM:NF015736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HET7056886.1"
                     /translation="MSLDRGTCSDRGAAGTAPAALRSRAFIAASSSNRGPVEAVDEWT
                     ADTADGVAATRSLVSVSRDLDQYAEEFAQRLLPIPTGFDVLDHWIGNGLRQGELLLLG
                     GAPGVGKTTFCLQIARNIAAGGHANVLYACYEHPEEYVLNRLLVQETIDPGAPLAAGG
                     LTLEELQLLSAEIAMTNGGGGFYETLRDHPAGARATSAIDEYAGRFHLVANAARENGI
                     RTLQALVAEHRARSDEPLVLFVDYLQKVEPAWGDVTDEDERVTEVVQALKQMALEHNV
                     AIVAIAASDKSGIEAPRLRFRHLRGSSALLYEADIAVIMNDKWSIIDRDHIVFNQHKA
                     QQYRDWVVCTVEKNRGGRNLVEIEFMKRFEYCCFNPRGGRVTERLYEERIQGES"
     gene            2075..>2655
                     /locus_tag="VFI12_10520"
     CDS             2075..>2655
                     /locus_tag="VFI12_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056887.1"
                     /translation="MPQHSGINRRALCIGLGVALIGGVTKVGLSGGSAGAAIVQVDAA
                     TTATRTAELGELSELRTQVAILSNATDCLPPGAATPDATPALISPVAMHEPIPYGEDW
                     TVTANTVTMRPTFGIFTATGVYAQVSLTITNNTSASRSFPYEELVLRDEQGRIFIPAQ
                     NVRTLNEAGWYSPFPPNLPTGGFIVFDVATDAQG"
BASE COUNT          459 a          893 c          822 g          481 t
ORIGIN      
        1 acctgtgccc agccgacccc ggtcgttcga agcgggacga tgcggcggcg catcgtcccc
       61 ggtgaaccta gagcactgct gtggctgaat ccagggcggc ggtaaccgca tcccacagcg
      121 cgttgatcgc atcccgcgcc aaaagcaggg cggcgatggc ggcgaccgag atcaggccga
      181 cgaggagggc gtactcaacc atgtcctggc cctcttcctc gcgaatgacc cggttcagga
      241 acgaaacgta tgcttccatg actgtgactc cttcgcgggc ccgacccgct cccaccgaaa
      301 aacgctgcgg cccttgcgaa ccagtgctca gcgggtagac ccgcaccaca acccgagcgc
      361 tcccccacgg ccgcgaacat cgcgggaccg cccgtgctgc caaccaacaa cgactccggc
      421 gaacccaacc aggttggcaa tggttgcgac ccgtcccggc cgcctgtcgt tgttcgttga
      481 tctcaatcta ggccgaaggt cctgcccggt catgagtgct cttccacggg cttgataggg
      541 gaatctccct accccgggtc ggcggatgtc agatgccggt cgtcggtttg gcgcgagcag
      601 gctttggagc cgcgtcatgc cggacacgag tcgaaacccc ggccggttca gtgcgacctg
      661 ccggggtttc gtccgaaaga gatggaagtt tcagatcagg attcgccctg gattcgttcc
      721 tcgtagaggc gctcggtgac gcggccgccg cgcgggttga aacagcagta ttcgaaccgt
      781 ttcatgaact cgatctcgac caggttccgg ccgccccggt tcttctcgac ggtgcacacc
      841 acccagtcgc gatactgctg agccttgtgc tggttgaaga cgatgtggtc gcggtcgatg
      901 atgctccact tgtcgttcat gatcaccgcg atgtcggcct cgtagagcag ggccgacgac
      961 cctcggagat ggcggaagcg aagacgcggc gcctcgatcc cgctcttgtc cgacgcggct
     1021 atcgcgacga ttgcgacgtt atgctccagc gccatctgct tcagcgcctg aacaacttcg
     1081 gttacgcgct catcctcgtc ggtcacgtcg ccccatgctg gctcgacctt ctggaggtaa
     1141 tccacgaaga ggaccagggg ctcatcgctc cgggcgcggt gctcggcgac gagcgcctgc
     1201 aatgtccgga tcccattctc ccgggccgcg ttcgcgacga gatggaaccg gccggcgtac
     1261 tcatcgatcg ccgaggtcgc ccgcgcaccg gcgggatgat cacgtaatgt ctcatagaat
     1321 ccaccgccgc cattcgtcat ggcgatctcg gcgctcagga gttgcaattc ttcaagcgtc
     1381 agcccacccg ccgcgagcgg cgcgccggga tcgatcgtct cctgcaccag caggcggttg
     1441 aggacgtact cctcgggatg ctcatagcag gcgtagagga cgttggcgtg tccgccggca
     1501 gcgatattcc gcgcgatctg caggcagaac gtcgtcttgc cgacgcccgg cgcgcccccg
     1561 agcagcagca gttccccttg ccggagtccg ttcccgatcc agtggtccag cacgtcgaat
     1621 ccggtcggaa ttggcagcag ccgctgcgcg aactcctcgg cgtactggtc gaggtcgcga
     1681 gaaaccgata ccagcgagcg ggtcgcggcg acgccatcag cagtgtcagc ggtccactcg
     1741 tcgaccgcct cgaccggtcc acgattcgac gacgaggcag caatgaatgc gcgcgatcgc
     1801 aatgcggccg gtgcagttcc cgccgccccc cggtcggaac atgttccgcg atcgagactc
     1861 atccgcgttc ctttcgtgca tcgaacaggg cagcccagga gtcttgccga cacgtggatt
     1921 tcacgcgcgg agatggcccg tttccactct atagcggtgt cggcccaacc tcgtgggggt
     1981 tactgcgccg gccgttaggg gattttgccc atccgttctg ccccagcgcc gggcatgatt
     2041 gcgtttcgat gctcgctacg gaacggacac ggtcatgcct cagcactcag ggatcaatcg
     2101 ccgcgcactc tgtatcgggc tcggcgttgc gctcatcggc ggagtgacca aagtcggctt
     2161 gagcggtggt tctgcgggag cggccattgt ccaggtcgac gcggcgacga ccgctacccg
     2221 gaccgcggag cttggcgaac tgtcagagct tcggacccag gtggcgattc tctccaacgc
     2281 cacagactgc ctgcctcctg gcgccgcaac accggatgcg acaccggcgc tgatctcgcc
     2341 ggtcgcgatg cacgagccga tcccgtatgg cgaggattgg actgttaccg ccaacaccgt
     2401 caccatgcgg cctacgttcg gcatctttac ggcgaccgga gtctacgccc aggtcagcct
     2461 gacgattacg aacaacacga gcgcgtctcg gtcattcccg tacgaggagc tggtgctgcg
     2521 cgacgagcag ggtcggatct tcatccccgc ccagaacgtc cgcaccctga acgaggccgg
     2581 ctggtactcg ccgtttccac cgaacctgcc gaccggcggg ttcatcgtct tcgacgtcgc
     2641 gacggacgcg caggg
//
LOCUS       DASSGX010000580         3422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_535564, whole genome shotgun sequence.
ACCESSION   DASSGX010000580 DASSGX010000000
VERSION     DASSGX010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535564"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..646
                     /locus_tag="VFI12_10525"
     CDS             230..646
                     /locus_tag="VFI12_10525"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HET7056888.1"
                     /translation="MTPDELKDCARRFFAEIWNARDESAIDRFIPENAKGNDADFGSG
                     REGFRKQWREWMDAFPDLHFEIVDMVAEGNKVLTRWVLTGTHSDAPFLGVEPAGTKIR
                     VEGMSLDRIDDGWVAEGFDGWDALGLRRQLGLYDGA"
     gene            complement(650..1027)
                     /locus_tag="VFI12_10530"
     CDS             complement(650..1027)
                     /locus_tag="VFI12_10530"
                     /inference="COORDINATES: protein motif:HMM:NF014136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET7056889.1"
                     /translation="MNIIAADEIELIETPNGNRGAALATSSHGALEVSVIRQRQSPGG
                     LNPPHTHDREEVMTVLAGRLRLTVEGEAGVVAAGDTAIIPARATHQVENAGDLEAEWL
                     LIAPAGIRFFHANGDEATPPWSK"
     gene            1130..1576
                     /locus_tag="VFI12_10535"
     CDS             1130..1576
                     /locus_tag="VFI12_10535"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET7056890.1"
                     /translation="MRSQDEIRETGRQHHVGRLLLRANRDFSTRAIQRLHALGYSGVT
                     VAHLSLLPNLDRTGARVTTLATRAGMTKQGMGQLVAELERQGYLTREPDPVDKRAALV
                     CFTASGLALLNDAIFVTRQIEDEYARVLGRERFGDLKQALTDLVEN"
     gene            complement(1587..2897)
                     /locus_tag="VFI12_10540"
     CDS             complement(1587..2897)
                     /locus_tag="VFI12_10540"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET7056891.1"
                     /translation="MSDLPNSNSPPQSSNDHVRRRHKRSYFALISSSFVSNTGNAFSN
                     LAIPLFVLATTGSATRTGIVAFVNYTPPVIAAIFGGALVDRIGRRRTLMTADALSGIA
                     TALIPLLYMADLLSFPLLLAIVAFGAFLDSPGRAARSSMIPGLADRAGYSPERAQSLS
                     MSGFFLSQVIGPAVAGLVVAASGPTTAIWVNAVTFVLSLAIVGNIVHEPEIATPVKKS
                     TYLEDLREGFHFVWHDGFMRAMIILASAFSMVFIPLYTVLYPVYFTRLIGSTRALGFF
                     LGAEAAGNLIGAIAYGYIGERFSRFNAFVFSLAAWVPFFAVMLFTPPVWLLLVAGFLS
                     SLASGAINPIFQVAFQVRTPESMRPRVYSMVTAGNLMAVPVGALLIGPLIELIGVIPT
                     LGIVVALGAAAPVVCFLVPVFREIDTPIELTEPMFEERALEPVT"
     gene            3039..>3422
                     /locus_tag="VFI12_10545"
     CDS             3039..>3422
                     /locus_tag="VFI12_10545"
                     /inference="COORDINATES: protein motif:HMM:NF020905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HET7056892.1"
                     /translation="MSLHVVTARKGAGYRLDTGQAIRIVNTHGTQVVDFWAVSLDDPS
                     TYLSMVHTRSTLFTAYPKAGDLLYDNRRAPILRFVEDTSPGIHDTVIAPCDPERYRML
                     GVEGFHDSCANNFLTATRELNLPFAQ"
BASE COUNT          668 a         1076 c         1090 g          588 t
ORIGIN      
        1 gcgacgaaaa cgctcgcgtc ttcatggact ctttctccgg gctgcgcgtt tgccggacgt
       61 tccgggaaga cgcgccaacc tcactatcta tactcaaagg gattatgcgg accggtgaac
      121 caaacggaag cttcgtatca tccagcggcg ttacaatttc gcaacgattc ccgactcaac
      181 caaccagagt gctgtcagtg tttcgacaag agtctgcatg gaggaccagg tgacacccga
      241 cgagttgaag gactgtgcgc ggcgattttt cgccgagatc tggaatgcgc gggatgagtc
      301 ggcaattgat cgctttatcc ccgagaacgc aaaggggaac gacgccgact tcggcagcgg
      361 ccgcgagggc ttccggaagc agtggcggga gtggatggac gcctttccgg atctccactt
      421 cgagatcgtc gacatggtcg ctgaaggcaa caaggtgctg acgcgctggg tcctcaccgg
      481 cacgcacagc gatgcgccct tcctgggcgt cgagccggcc gggacgaaga ttcgggtcga
      541 ggggatgagc ctggatcgga tcgacgacgg ttgggtggcc gagggcttcg acggctggga
      601 tgccctcggc ctgcgccgcc agcttggcct ctacgacgga gcctgacggc tatttggacc
      661 agggtggcgt agcttcgtcg ccatttgcat ggaagaaccg aatcccggcc ggcgcgatca
      721 ggagccactc agcctcgagg tcgccggcgt tctcgacctg atgcgtcgct cgtgccggga
      781 tgatcgccgt gtccccggcg gccaccaccc ccgcctcgcc ctccactgtc aaacgcagcc
      841 gtcccgcgag gacagtcatg acctcctcgc ggtcgtgggt gtggggcggg ttcagtccgc
      901 ccggggactg gcgctgccga atcacactga cctcgagagc gccgtggctc gatgtcgcca
      961 aagccgcacc ccggttgcca tttggtgttt cgatgagctc gatttcgtcc gctgcgatga
     1021 tgttcactgc cacactcccg ttcgctaaca cgcctgtgat agaatagaca agtagcttgt
     1081 ctatctgaga tagtaaagtc tatttcctat gccgtcaagg ggccacgatg tgaggagtca
     1141 ggacgaaatc agggaaaccg gtcggcaaca ccatgtcggg cgcctcctcc tccgcgcgaa
     1201 ccgcgacttc agcacgcgcg ctatccagcg attgcacgcc ctcggatact ccggcgtcac
     1261 tgtcgcgcac ctcagtcttc tgcccaacct tgatcgaacc ggggcgcggg tgaccaccct
     1321 cgcgactcgg gcggggatga ccaagcaggg gatggggcag ctcgttgcgg aactcgaacg
     1381 ccagggttat ctgacccgcg agcccgaccc ggtcgacaag cgggcagcac tcgtctgttt
     1441 cacggcgtcc ggactcgcgc tcttgaacga cgcaatattt gtcacgcggc aaatcgagga
     1501 cgaatatgcc cgggttctcg ggcgagagag gttcggtgat ctgaagcagg cgctcactga
     1561 cctggtcgag aattgacgcc cagctgtcaa gtaaccggct cgagggcgcg ctcctcgaac
     1621 attggctccg tcagttcaat cggcgtgtcg atctcgcgga agaccggcac caggaagcaa
     1681 acgaccggcg cggctgcccc gagcgcgacc acgatcccaa gcgtcgggat tacgccgatc
     1741 agctcgatca gcgggccgat cagcagcgcg ccgaccggga ccgccatcag gttgccggcc
     1801 gtgaccatgc tgtagacccg aggccgcatc gactccggcg ttcgcacctg gaacgcgacc
     1861 tggaaaatcg ggttgattgc gcccgaggcg aggctggaga ggaatccggc gaccaggagc
     1921 agccagaccg gcggcgtaaa cagcatcact gcgaagaacg gcacccaggc ggccaggctg
     1981 aagacgaatg cgttgaaccg gctgaaccgc tctccgatat agccgtatgc tatggcgccg
     2041 atcaggttcc cggctgcttc cgccccgagg aagaagccga gcgcgcgcgt gctgccgatc
     2101 aggcgcgtga agtagaccgg gtagagcacg gtgtacagcg ggatgaagac catcgagaag
     2161 gccgacgcca gaatgatcat cgcccgcatg aatccgtcgt gccagacgaa gtggaatccc
     2221 tcgcgcaggt cctcgagata ggttgacttt ttgaccggcg tcgcgatctc cggctcgtgg
     2281 acgatgttgc cgacaatcgc cagcgagagc acgaacgtga ccgcattcac ccagattgcc
     2341 gtcgtcgggc cggatgcggc cacgaccagg ccggccaccg ccgggccgat cacctgcgac
     2401 aggaaaaagc cggacatgct gagcgactgt gcccgctccg gcgagtagcc ggcccggtcc
     2461 gccaatccgg ggatcatcga cgaccgcgcc gcccggccgg gactgtcgag gaacgcgccg
     2521 aaggccacga tcgccaacag cagcgggaaa ctgagcaggt ccgccatata gaggagcggg
     2581 atgagcgcgg tcgctatgcc gctcagggcg tcggccgtca tcaatgtccg ccgtcgcccg
     2641 atcctgtcga ccagcgcccc gccgaagatc gcggcgatga ccggcggcgt gtagttgacg
     2701 aaagcgacga tacccgttcg cgtcgcgctg ccggtcgtgg ccagcacaaa gagtggaatt
     2761 gcgaggttcg agaaggcgtt gccggtgttc gagacgaatg acgacgagat cagcgcgaag
     2821 taggagcgct tgtgtcgccg gcggacatgg tcgttgctcg actgaggtgg cgagttcgag
     2881 ttgggaagat cagacaagcg agcgcggtcc cctttcgcgc atgccaacct actcgccgct
     2941 atgcgtcatc gtcaaccgtc gaacgaccca tcagaatgcg atgatgtcgt acctgatcgc
     3001 cgggtatcga ggatcgctca tcaacgagga gaacggagat gtcactgcat gttgttacgg
     3061 cgcggaaggg ggccggatat cggctcgata ccgggcaggc gatccgaatc gtcaacacgc
     3121 acggcacgca ggtggtcgat ttctgggccg tgtcgctgga tgacccgtcg acctaccttt
     3181 cgatggtcca cacccgctcg acgctcttca ccgcctatcc gaaggccggt gatctgctct
     3241 acgacaaccg ccgcgccccg attctccgct tcgtcgagga caccagtccc ggtatccacg
     3301 acaccgtgat cgcgccctgc gatcccgagc gctaccggat gctcggcgtc gagggcttcc
     3361 acgacagttg cgccaacaac ttcctgaccg ccacgcggga gctgaatctt cccttcgcgc
     3421 ag
//
LOCUS       DASSGX010000581         2766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_865499, whole genome shotgun sequence.
ACCESSION   DASSGX010000581 DASSGX010000000
VERSION     DASSGX010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865499"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VFI12_10550"
     CDS             complement(<1..641)
                     /locus_tag="VFI12_10550"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET7056893.1"
                     /translation="MKHATGISALASPLISQNAIDRSARTDVIVVGAGVAGLAFALSV
                     SPDCHVVLLTKGHLGESNTRWAQGGLSAAIGTDDTIALHEQDTLYAGAGLSDPETVRE
                     LVADGPEAVDWLLSLGTAFDRDEETGELALGREAAHSRRRVLHAGGDATGAEIERALV
                     AAVRSRPNIKILESSFAIDLIITDGTCTGATVALRSGEIVAIEAQAVVLAAGGA"
     gene            complement(716..1231)
                     /locus_tag="VFI12_10555"
     CDS             complement(716..1231)
                     /locus_tag="VFI12_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056894.1"
                     /translation="MDNDFELERPIDLTISSLDEDIDIVERDRQALDPVRRSLVEAGF
                     YAYTTYDDQQRWTVAVDDEAGRVDVRVGMDGYHVELWGSDPGMFADEENEWKRRAQQR
                     LVRIMLPNIARGHLAEHQHAIWDEVDQGIAVRVTYELPFTRAHQIGAFVREHLPELEE
                     LLTLVESQIDV"
     gene            complement(1247..2437)
                     /locus_tag="VFI12_10560"
     CDS             complement(1247..2437)
                     /locus_tag="VFI12_10560"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET7056895.1"
                     /translation="MRQIEQLPDSADVVIIGGGIVGCASAFFLARSGRQPIVLEQMGA
                     IASATTSVSAHAIRCQFTEAENIAQTSESLAIYENFRDVIGDPAAQINLTQNGYLFAS
                     TDEGDIPLFRDRVARQHALGVSDVQLLTGDDIRYRFPWMAEEITVGTFRQRDGWIDSV
                     LATNHFLAAAGAPVALDTTVTAIEASGGRVIGVMTNRGRIATDTVVLAAGPFSRDISP
                     EPLPLARWRRHRIVLDADERIPQGAPMTIDANTGAHWRPHAGGALMAWAQPETDRAAT
                     WPVERDPTWPDLILRSDRGIARLCPFWNEITPGLAPDDYLFTAGLYTVTPDHKPLIGP
                     ANETDGLFLNTGYSGHGIMGSPSGSRLLADLLAGTVSSADNPFHPGRFATGAKPPDVE
                     KIVL"
     gene            complement(2585..>2766)
                     /locus_tag="VFI12_10565"
     CDS             complement(2585..>2766)
                     /locus_tag="VFI12_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056896.1"
                     /translation="YSRELSDMTSLLMFTGAPAGGPAFVPTLGPFTISCASPGKTPNR
                     NGWTAPAKKQNLGSN"
BASE COUNT          470 a          887 c          900 g          509 t
ORIGIN      
        1 gcgccgccgg cggccaacac gaccgcctgt gcctcgatcg ccacgatctc gccgcttctg
       61 agtgcgaccg tcgcgccggt gcatgtgcca tcggtgatga tcaggtcgat cgcaaagctg
      121 gattcgagga tcttgatgtt cgggcgcgat cggaccgccg caacgagggc gcgctcgatt
      181 tctgcgccgg tcgcgtcgcc gcccgcgtgc agcacccgcc gccggctatg cgctgcctcc
      241 cgcccgagcg ccaattcgcc ggtctcttcg tcgcgatcga acgcggttcc gagcgagaga
      301 agccagtcga ctgcctcggg cccatctgcg accaactccc ggaccgtctc cgggtcgctc
      361 agtccggcgc cggcatagag cgtgtcctgc tcatgcaacg cgatcgtgtc atcggtcccg
      421 atcgccgctg agagcccgcc ctgggcccag cgagtgttgc tttcgccgag atgccccttg
      481 gtaagcagga ccacgtggca atcgggcgag accgacagcg caaaggcgag gccggcgacg
      541 cccgcgccga cgacgatgac gtcagtccgc gcgctccggt cgattgcgtt ctgactgatc
      601 aagggggacg cgagcgctga gatgccggtt gcgtgcttca acgggttctg cctcggatag
      661 ggtcggaaaa cacgattcca gggtgaacga taccggattc agccggccgt tatcctcaaa
      721 cgtcgatctg tgactcgacc aatgtcagta gctcttccag ctctggcagg tgctcgcgca
      781 cgaacgcgcc gatctggtgc gcgcgggtga agggcagctc atacgtgacg cggactgcga
      841 tgccctggtc gacctcatcc cagatcgcgt gctggtgctc ggccaggtgg ccgcgggcga
      901 tgttcggcag catgatccgg accagtcgct gctgggcccg ccgcttccac tcgttctcct
      961 cgtcggcgaa catgcccggg tcggaacccc acaattcgac gtggtagcca tccatgccga
     1021 cgcgaacgtc gactcgccca gcttcgtcat cgacggccac ggtccagcgc tgctggtcgt
     1081 cgtaggtcgt gtacgcgtag aacccggcct cgacaagtga gcgccggact ggatccaggg
     1141 cctgtcgatc gcgttcgacg atgtcgatat cctcgtcaag tgacgagatg gtcagatcaa
     1201 ttggtcgttc gagttcgaag tcgttgtcca tgatccagca tcggtgttac agcacgatct
     1261 tctcgacgtc cggtggcttg gctccggtgg cgaagcgacc cgggtggaac ggattgtctg
     1321 cgctgctcac cgttccggca agaagatcgg cgagcaaccg gctaccggac ggcgagccca
     1381 tgatgccgtg cccgctgtaa cccgtattga ggaagaggcc gtcggtttca ttagccgggc
     1441 cgatcagcgg cttgtggtcc ggggtgaccg tgtagagacc ggcggtgaac aggtaatcgt
     1501 ccggcgcgag cccgggcgtg atttcgttcc aaaaggggca aagtcgcgca atcccccggt
     1561 ccgagcgcag gatcaggtcg ggccaggttg gatcccgctc gaccggccag gtcgccgccc
     1621 ggtccgtttc aggttgggcc caggccatca gcgcgccacc ggcatgaggc cgccagtggg
     1681 cgccggtgtt ggcgtcgatc gtcatcggcg cgccctgcgg gattcgctcg tcggcgtcga
     1741 gaacgatccg gtgtcgccgc cagcgcgcga gaggcagggg ctccggactg atgtcgcgac
     1801 tgaacggtcc ggccgcgagg acaaccgtgt ccgtcgcgat ccggccgcga ttcgtcatca
     1861 ctccaatgac acggccgccg cttgcctcga tcgccgtgac cgtcgtgtcg agggcgaccg
     1921 gcgcgcccgc cgctgcaagg aagtggttgg tcgccagcac gctgtcgatc cagccgtccc
     1981 gctgccgaaa cgtgccgacc gtgatctcct ccgccatcca cgggaagcga tagcgaatgt
     2041 catcaccggt cagcaattgc acgtcgctga caccgagcgc gtgctgccgc gcgacgcgat
     2101 cccggaagag cgggatgtcg ccttcgtcgg tcgaggcgaa aaggtagccg ttctgggtga
     2161 ggttaatttg cgccgccgga tccccgatga cgtcgcgaaa gttctcatag atggcgaggc
     2221 tctcgctcgt ctgcgcgatg ttctcagctt cggtgaactg gcaccggatc gcgtgggcgg
     2281 agacggaggt ggtggccgat gcgatcgcgc ccatttgctc cagaacgatg ggctggcggc
     2341 cggaacgcgc caggaagaag gccgacgcac acccaacgat gccgccaccg atgatgacca
     2401 catccgctga atccggaagc tgctcaatct gtcgcacagc gcttgcaccc gcttctgcta
     2461 tctggatacc gaagatcgcc gaatcctact gcgcggaatc cggccccgcc ggggcgtaat
     2521 cttcgcaaat ctgatttgac accctgttac aggctcggct attctccggc accaaggttt
     2581 accatcaatt gctgcccaga ttttgcttct ttgcgggagc agtccaaccg tttcggttcg
     2641 gtgtcttccc tggagacgcg caactgatgg tgaacggtcc caatgtgggg acaaaggcag
     2701 gaccaccggc gggtgcgccg gtgaacatca ggagagaggt catgtcggac agttctcgtg
     2761 agtaca
//
LOCUS       DASSGX010000582         5493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_974310, whole genome shotgun sequence.
ACCESSION   DASSGX010000582 DASSGX010000000
VERSION     DASSGX010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5493
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974310"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /locus_tag="VFI12_10570"
     CDS             complement(<1..1049)
                     /locus_tag="VFI12_10570"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HET7056897.1"
                     /translation="MPDKRQKTVMLIDGYGLIFRAYHAIDTAMSTSSGEQTNAVFGFT
                     RMLLDVLESQKPDYAIVALEGGKTFRHDEYDGYKANRGSMPDDLRAQVGRVRSIIDAM
                     NVPIIEREGFEADDVIGSLTHKLADEQKLHVLVITGDSDLLQLVEPGVEAVLPGRPRF
                     QDLRIYDVAAVEDRYGFSPSLIPDYKALVGDTSDNIPGVPGIGDKTAKALIQEYGDVE
                     AILANLESVKPPRARNALTENVDTLRLSKRLATIVTNLDIDPDLEHSAVDNFDRDEVI
                     DIFRELEFRSLVHRLPVSRQPSVAPVAVVERKAPTRTTATTDDQLAAVATRIRETGQY
                     AFDVETDSLDPLTANL"
     gene            1176..2159
                     /locus_tag="VFI12_10575"
     CDS             1176..2159
                     /locus_tag="VFI12_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET7056898.1"
                     /translation="MQIQRVQELAARVTGNVERVVVGKRREVELVLVALLCRGHVLIE
                     DVPGVGKTVLAKAVARSLGASFKRLQFTPDLLPGDVTGVSVFDPQHVRFEFRPGPVFA
                     QLVLADEINRATPKTQSALLEAMEEAQVTVDGVTHLLPEPFIVLATENPIDYEGTFPL
                     PEAQLDRFLMRISMGYPGRAGEIEMLERQQHQHPLERIGQVVDVANLVAAQIEVRDVR
                     VDSAVADYVVSIVESTRNHPDVQLGASPRGSLALYNASRALAALNGRTFVIPDDVKAL
                     AVATLAHRLIVSPAARVRNVDSRLIVQEIIRGLPVPGARPMAAAGRAQTVT"
     gene            2159..3406
                     /locus_tag="VFI12_10580"
     CDS             2159..3406
                     /locus_tag="VFI12_10580"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET7056899.1"
                     /translation="MVALRVLLIAIAGLVYALVDDWPAITMISSALLVVVVLSFIWSW
                     TSLSGLSFARRLSSDRAQVGDQLREELAVRNQSIFGKLFLEIEDRTTLPGHLANRVIK
                     LGPRSARQWRVESVARERGRHRLGPVTLRSGDPFGLFRRQKRLKETLDVTVYPLTVDL
                     EPYVPGSVLQSGGGVIQRRSSSPTTTVSTIRDYVQGDSINRVSWSATARAGRLMVKEF
                     ENDPTADVWIILDLYEPPTDGRLVGARFASDDPLNWMANDLEYRVMLAGSLARRALAL
                     GRSVGLIMNAAQPIVLPPERSDRQYVRILELLAVVEGRPDASLAELLTGLTARFRRDS
                     VLVAVTSDIDPNLIALLATVRQRRISSEVIFVDGHMHPSAETIARIEELQRERIAGYR
                     MTRADHPAGMLQVVPGTAGAWVG"
     gene            3431..>5493
                     /locus_tag="VFI12_10585"
     CDS             3431..>5493
                     /locus_tag="VFI12_10585"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HET7056900.1"
                     /translation="MPGTRATRPEYKAEGLALVLHAVLVLSVVASMNRVDLHDRLTVL
                     GPLALLGLLLGYALSRTRAQDLVAHSVALWTGAISALILVTVVSSGPVGIVHGRGRAF
                     LDLLRGVARSLTSNEADSVSDAELLVVLGVTAWLLAYSSAWVMYRRGWYGLGIAVPAS
                     ILLASIRVDQRGGGWPLALFVFAAIGLAARNAIALNSMRWARRNMSPGPGLTSRFLFA
                     GLPVAVIAVVAALLVNPGMHQALSVPYRETAQRGWDSVRDHVEQTLGKSGSGGGSYAS
                     FPDEFSIGGNIDLSNEVVAKVQADQGHYLALRRYDIYTGRGWKSGVQSTFRLDGDDPN
                     IRVTNVIFGESQAVALSGEVTGDRAPESALITVVRPKDDLIFTIETFSSASKQVYAVL
                     GWQRLNRTEIDVNAVDLGKVPVDLQGLVRAVRNAPFEVDRASGKVILTDADVQEAFDR
                     SRQRLASYPVNAELTVGDQGQLVVVISGRIPNYDDIEALFTSEAIRPDTDYRVVGLAS
                     IASPDQLASAPGDYPVWISDRYLQLPPSVTERTRAEAQRVAIQAGATNSFDKVWAIQD
                     YLRSNFSYELNSPGPPGNQDWVDYFLFDHQAGRCEQFASAMVVMVRSLGIPARLVNGY
                     NYSGETDAQGEVIYRENQAHTWVEVFFPEYGWVPFEPTTNQAEFSYGENGPQQDVPSP
                     EADPAT"
BASE COUNT          895 a         1727 c         1797 g         1074 t
ORIGIN      
        1 aggttcgcgg tcagcgggtc gagcgaatcg gtttcgacgt cgaatgcgta ctggccggtc
       61 tcgcgaatcc gcgtggcgac cgcagccagt tggtcgtcgg tcgtggccgt ggttcttgtc
      121 ggcgcttttc gttcgacgac cgcgaccggc gcgacagatg gctggcgcga aaccggcaga
      181 cgatggacga gcgatcggaa ctccagctcc cggaagatgt cgatcacttc gtcgcgatcg
      241 aagttatcga ccgcgctgtg ctcgaggtcg ggatcgatgt cgaggttggt gacgatcgtg
      301 gcgagtcgct tgctcaggcg cagggtatcg acgttctcgg taagcgcatt cctggcgcgg
      361 ggcggcttca cggattcgag gttggccagg atagcttcaa catcgccgta ttcctggatc
      421 agtgccttgg ccgtcttgtc gccgattccc ggcacgccgg ggatattgtc ggacgtgtcg
      481 ccgacgagcg ccttgtaatc cggaatcagg ctcgggctga acccataccg atcctcgacg
      541 gcagcgacgt cgtagatgcg caaatcctgg aatcggggac ggccgggcag caccgcctcg
      601 acgcccggct cgacaagctg gagcaagtca gagtcgcccg tgataacgag gacgtgcagc
      661 ttctgctcgt cggcgagctt gtgggtcaga ctgccgatca catcgtcggc ctcgaatcct
      721 tcgcgctcga tgatcgggac attcatcgcg tcgatgatcg agcggacccg gccgacttgc
      781 gcgcggagat cgtccggcat tgagccgcgg ttggccttgt agccgtcata ttcgtcatgg
      841 cggaacgttt tgccgccttc gagcgcgaca atcgcgtagt cgggcttctg cgattcgagg
      901 acatcgagca gcatgcgggt gaagccaaag accgcgttcg tctgctctcc ggatgaagtg
      961 gacatcgcgg tgtcgatggc gtggtaggcg cggaatatga gcccatagcc atcgatcagc
     1021 attaccgttt tctgacgctt gtccggcacg ccctgagtcc ctccacccgc agaatcctgg
     1081 gcagagtata cggcgtaggt catttgttcg cctgaggcgg ttggtatact tgtcttgcct
     1141 gtccgccacc gtccgagaag cgagcaatcc cgttcatgca gatacaacga gttcaggagc
     1201 tggctgctcg cgtcactggt aacgtcgagc gcgtggtcgt gggtaagcga cgcgaggtcg
     1261 agctggtcct cgtcgcgctg ctctgtcgcg ggcacgtgct gatcgaggac gtgccgggag
     1321 tcggcaagac ggtcctggcc aaggcggtcg cccggagcct cggcgcgtcg ttcaagcggc
     1381 tccagttcac gccggacctc ttgcccggcg acgtcacggg cgtgagtgtc ttcgacccac
     1441 agcatgtccg gttcgaattc cggcctggcc cggtcttcgc ccagctcgtc ctcgccgacg
     1501 agattaaccg ggccacgcca aagacccaat ccgcgttgct cgaggccatg gaagaggcgc
     1561 aggttacggt ggacggcgtc acgcatctcc tgcccgagcc gttcatcgtc ctcgcgaccg
     1621 agaatccgat cgactacgaa ggcaccttcc cgctgccgga ggcgcagctt gaccgtttcc
     1681 tgatgcggat ttcgatgggc taccccggcc gcgccggtga gatcgagatg ctggaacgtc
     1741 agcagcacca gcacccgctc gagcggattg gacaggtggt cgacgtagcc aacctggttg
     1801 cggcgcagat cgaagtgcgt gacgttcggg tcgactcggc ggtggccgat tacgtcgtct
     1861 caatcgtcga gtcgactcgc aaccatcccg acgttcagct cggcgccagc ccgcgtggca
     1921 gcctcgcgct ctacaacgcc agccgcgccc tggccgccct gaacgggcgc acgttcgtga
     1981 tcccggacga cgtcaaggcg ttggccgtcg ctacgttggc gcaccggttg atcgtctcgc
     2041 ccgccgcccg cgttcgcaat gtcgatagcc gtctgatcgt gcaggagatt atccgcgggc
     2101 ttccggtccc cggcgcccgc ccgatggcgg cggccgggcg cgcccagacc gtcacctgat
     2161 ggtcgcgctg cgcgttctcc tgatcgcgat cgccgggctt gtctacgccc tcgttgacga
     2221 ctggcccgcc atcaccatga tctcgtcggc gttgctcgtc gtggtcgtgc tgtcattcat
     2281 ctggtcctgg acgagccttt cggggctgtc ctttgcccgg cgcttatcgt cagatcgcgc
     2341 gcaggtcggg gaccaattgc gcgaagagct ggccgtgcgg aaccagagca ttttcggcaa
     2401 gctcttcctc gagatcgaag atcgaacgac actgcccggt cacctggcca accgcgtgat
     2461 caagctcggc ccacgatcgg cgcgccagtg gcgggtcgaa tccgtggcca gggaacgcgg
     2521 ccggcaccga ctcgggccgg tcacacttcg ctccggcgac ccgttcggtc tcttccggcg
     2581 gcagaagcga ctgaaggaga cgctcgacgt cacggtgtat ccgcttaccg tcgacctcga
     2641 gccgtatgtc cccggctccg tgctccagtc gggcggtggc gtgattcagc gccggtcgag
     2701 cagcccgacc acgacggtca gcacgatccg cgactacgtg cagggcgatt cgatcaaccg
     2761 ggtgagctgg agtgcgacgg ctcgggcggg acggctgatg gtcaaggaat tcgaaaacga
     2821 cccgacagcc gacgtctgga tcatcctcga cctttatgag ccgccgaccg atggacgtct
     2881 ggtcggcgcg cggttcgcat ccgatgaccc gctgaactgg atggcgaacg acctcgagta
     2941 ccgcgttatg ctggccgggt cgttggcccg ccgcgcgctt gccctcggtc gatcggtcgg
     3001 gttgattatg aacgcggcgc aaccgatcgt tttgccgccg gagcgcagcg atcgccaata
     3061 cgtgcgaatc ctcgagctgc tcgcggtcgt tgaaggccgg ccggacgcgt cgcttgcaga
     3121 gttgttgacc ggtctgaccg cgcggttccg gcgcgacagc gtgctggtcg ccgtgacgtc
     3181 cgacatcgat ccgaacctga tcgctcttct ggcaacggtg cgccagcgca gaatctcatc
     3241 cgaggtaatt ttcgtagacg ggcatatgca tccgtctgcc gagacaattg cccgcatcga
     3301 ggaacttcag cgcgagcgca tcgccggcta ccggatgacc cgggccgatc atcctgccgg
     3361 gatgttgcaa gtcgttccgg gtacggccgg cgcgtgggtt gggtagggta tggcaagcct
     3421 gagcagcgca ttgcctggga ccagggcgac ccggcccgaa tacaaggcgg aggggctcgc
     3481 gctcgtcctc catgccgtgc tggtcctgag cgtcgtggcg tccatgaacc gggtcgatct
     3541 ccacgatcgg ttgaccgttc tcggacctct ggcactgctc ggcctgctcc tcggctatgc
     3601 attgtcccgg actcgagccc aggacctggt tgcccactcg gtggcgctct ggaccggcgc
     3661 gatttccgcc ctgatcctcg tgacggtcgt ttcctcgggt ccggtcggaa tcgttcacgg
     3721 tcggggccgg gcgtttctgg atctgctccg tggagttgcc cggtcgttga cgtccaacga
     3781 agcggacagc gtcagtgatg ccgagctgct ggtcgtgctc ggggtcaccg cctggctcct
     3841 cgcctacagc tcggcgtggg tgatgtaccg ccgcggttgg tatgggcttg gcattgcggt
     3901 gccagcctcg atcctgctcg catcgatccg ggtcgatcag cgcggtggtg gttggccgct
     3961 ggccctgttt gtcttcgcag ccatcggcct tgcggctcgg aacgcaattg cactgaactc
     4021 gatgcgctgg gctcgccgca acatgtcgcc aggaccgggc ctgacctcaa ggttcctctt
     4081 cgcgggtctt cccgttgcgg tgattgcggt cgtcgccgcc ctgttagtca acccgggcat
     4141 gcatcaggcg ctatccgtcc cctaccggga gacggcgcag cggggctggg attcagtccg
     4201 ggaccatgtc gagcagacgc ttggcaagtc cggttcgggt ggaggcagct acgcgagctt
     4261 cccggacgag ttctcgatcg gcggcaacat cgaccttagc aatgaagtgg tagcgaaggt
     4321 gcaggccgat cagggccact acctcgcgct ccgccggtat gacatataca ccggtcgagg
     4381 ttggaagtcg ggcgtgcaat cgacgttccg acttgacggc gacgatccca acatcagggt
     4441 gacgaacgtc atcttcggag aatcgcaggc ggtcgcgctt tccggcgagg tgacgggtga
     4501 ccgcgctccg gagtccgcac tgatcaccgt cgtccggccc aaggacgacc tcatcttcac
     4561 aatcgagaca ttctcgtccg cttcgaagca ggtctacgcc gtgctcggct ggcagcggct
     4621 taatcggacc gagatcgacg tgaacgccgt tgacctcggc aaagtcccgg tcgatctcca
     4681 gggtctcgtc cgcgcggtcc ggaatgcgcc atttgaggtc gatcgcgcgt ccggcaaggt
     4741 catcctcacc gacgcggatg tccaggaggc gttcgaccgc tcgcggcagc gattggcgtc
     4801 atacccggtc aatgccgagt tgacagtcgg agaccagggc cagctcgtcg ttgtcatttc
     4861 gggacggatt ccaaactacg atgacatcga ggccctgttc acgagcgagg cgatccgtcc
     4921 ggacacggat taccgggttg tcggactggc cagtattgca agcccggatc agctcgcatc
     4981 ggcgccgggc gattatccgg tctggatttc cgatcgatac ctccagctcc cgccgtcggt
     5041 cacggagcgg acccgagccg aggcgcagcg tgtggccatc caggccggtg cgacgaactc
     5101 gttcgacaag gtgtgggcga tccaggacta tctgcgctcc aacttctcgt acgagctcaa
     5161 cagcccggga ccacccggga atcaggactg ggtcgactat ttcctcttcg atcatcaggc
     5221 aggtcgatgc gagcagttcg cgtcggcaat ggtggtgatg gtgcgctcac ttggaatccc
     5281 ggcgcggctc gtcaatgggt acaactactc gggcgagact gacgctcagg gcgaggtgat
     5341 ctaccgcgag aaccaggcgc acacctgggt cgaagtcttc ttccccgaat acggatgggt
     5401 tcctttcgaa ccgaccacga accaggccga gttcagttat ggggagaatg ggccccagca
     5461 ggatgttcca tcgccggaag cggacccggc gac
//
LOCUS       DASSGX010000583         2528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_102139, whole genome shotgun sequence.
ACCESSION   DASSGX010000583 DASSGX010000000
VERSION     DASSGX010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102139"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VFI12_10590"
     CDS             <1..397
                     /locus_tag="VFI12_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056901.1"
                     /translation="PDNPADPVIKTLGESTALVYIRGTEKDADELIRRFRHSPKPMYY
                     QPPFLTRKWAEFKKLNKIADDNKVDPDAFGAWGFEALLHDRLPRYQKLADKYGYTIEA
                     ADLATVRDGEEFVDLVSTAIKGRMRSGRG"
     gene            480..1394
                     /gene="metA"
                     /locus_tag="VFI12_10595"
     CDS             480..1394
                     /gene="metA"
                     /locus_tag="VFI12_10595"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: protein motif:HMM:TIGR01001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HET7056902.1"
                     /translation="MPIRIPDHLPARKTLEHEGVVVMDSSRASRQDIRPLQIGLLNLM
                     PNKERTETQFSRLIGATPLQVDLTLVRVTDHKSKNTSEDYLKTFYSTWEDVRARKFDG
                     FIVTGAPIAHLPFEEIKYWPEMVEIMKWTQTHVHHTMFICWGAQAALHHFHGAERYRM
                     PQKAFGVFRHKVLNPRSAWLRGVSDSPMIPVSRYNDIDRRTLGPDLEILIDNDEIGVC
                     FIDDPKHRAVHMLNHLEYDNRSLADEYERDLKAGLNPVLPVNLFPNGDTTVDPENRWR
                     SHAHLLFQNWINEIYQTTPFDMAKIGEG"
     gene            complement(1519..1956)
                     /locus_tag="VFI12_10600"
     CDS             complement(1519..1956)
                     /locus_tag="VFI12_10600"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HET7056903.1"
                     /translation="MDANADAPVVEVETTIAASPDKVWAAMTNRTSPMFMGATMDTDW
                     QPGSKYTLHGDWQGHAFTDYGVIETAVPGKELSFTHWAKTPEPPESFNAVKYTLTPQG
                     GQTKVTLGQYARGKAQNFDDKTKAEFKKNWAMMLDGLKAAAEK"
     gene            2096..>2528
                     /locus_tag="VFI12_10605"
     CDS             2096..>2528
                     /locus_tag="VFI12_10605"
                     /inference="COORDINATES: protein motif:HMM:NF016224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HET7056904.1"
                     /translation="MPQSPTSFADEIGIALHNLADAAAGSVTPTLRIGVTGLSRAGKT
                     IFITALIHNLLAGGRLPGFAVLTEGRFIGARLAEAPDPGVPRFAYEQHLAALTGSTPV
                     WPESTRRISELRLTLKFQSERWFAGWTGPSTLNLDIVDYPGE"
BASE COUNT          484 a          907 c          701 g          436 t
ORIGIN      
        1 ccccgacaac cctgccgacc cggtgatcaa gacgctgggc gaaagcaccg cgctggtcta
       61 catccgcggc accgaaaagg acgccgacga gctgatccgc cgcttccggc actcgcccaa
      121 accgatgtat taccagccgc ccttcctcac caggaaatgg gcggagttca agaagctcaa
      181 caagatcgcc gacgacaaca aggtcgatcc cgacgccttc ggcgcttggg ggttcgaggc
      241 gctgctgcac gatcgcctgc cccgctacca gaagctcgcc gacaaatacg gctacaccat
      301 cgaggcggcc gatctcgcga ccgtccgcga cggcgaggaa ttcgtcgatc tcgtctcgac
      361 cgcgatcaag ggccgaatgc ggagcggccg cggttgaccg aacgagccgt cttcggatcg
      421 ttcggacaga gcaaccggaa aagctgagcc gtccgcgtca tcccaactga gatcgcatca
      481 tgcccattcg cattcccgac caccttcccg cgcgcaagac tctcgaacac gagggcgtcg
      541 tcgtcatgga ttcctcccgc gcctcgcggc aggacatccg accgctgcag atcggcctgc
      601 tcaacctgat gcccaataag gagcggaccg aaacgcagtt ctcgcgcctc atcggcgcga
      661 cccccctgca ggtcgatctg accctggtcc gcgtcaccga ccacaaatcc aagaacacgt
      721 ccgaggacta tctcaagacc ttctactcga cctgggaaga cgtccgcgcg cgcaagttcg
      781 acggcttcat cgtcaccggt gccccgatcg cccatttgcc tttcgaggag ataaaatact
      841 ggcccgagat ggtcgagatc atgaagtgga cgcagaccca cgtgcaccac accatgttca
      901 tctgctgggg cgcccaggcc gcgctgcacc actttcacgg cgccgaacgc taccgcatgc
      961 cgcaaaaggc cttcggcgtc ttccggcaca aggtcctcaa tccgcgctcg gcatggctcc
     1021 gcggcgtctc cgacagcccg atgatcccgg tcagccgcta caacgatatc gaccgccgga
     1081 ccctcggtcc cgacctcgag attctgatcg acaacgacga gatcggcgtg tgcttcatcg
     1141 acgaccccaa gcaccgggcc gtgcacatgc tcaaccatct cgaatacgac aaccggtcgc
     1201 tcgcggacga atacgagcgc gacctgaagg ccggtctcaa ccctgtgctt cccgtcaacc
     1261 tcttccccaa cggcgacacc accgtcgatc ccgagaaccg ctggcgcagc cacgcccatc
     1321 tgctgttcca gaactggatc aacgaaatct accagaccac gccgttcgac atggcgaaga
     1381 tcggcgaggg ttgagctcaa tcgctccatc gtcatggccg ggcgaagccc cggccaacca
     1441 gatggaagcg cgtcgcgcgc cgaaggcttg gctggggtcc gcggatcaag tccgcggatc
     1501 gcggagcaaa gagatcggtt atttctccgc cgccgccttc agcccatcca gcatcatcgc
     1561 ccagttcttc ttgaactcgg cctttgtctt gtcgtcgaag ttctgcgcct tgccgcgggc
     1621 atactgcccg agcgtcacct tcgtctggcc gccctgcggc gtgagcgtat atttgaccgc
     1681 gttgaagctt tcgggaggct ccggcgtctt cgcccaatgc gtgaagctca gctccttgcc
     1741 cggcactgcg gtctcgatga cgccgtagtc ggtgaacgcg tggccttgcc agtcgccgtg
     1801 cagcgtatat ttcgagcccg gctgccagtc ggtgtccatc gttgcgccca tgaacatcgg
     1861 cgaggtgcgg ttcgtcattg ccgcccagac tttatcgggc gatgccgcga tcgttgtttc
     1921 gacctcgacc accggcgcgt cggcgttggc gtccatgcat tcctcctcaa gcgaacaaaa
     1981 agagaacaac tgtccccggc ataagtcaag aaccccgtcg caacggccct ccctgcagag
     2041 cggttgcttg catttgccga ctgaagacct acgtcatcgc gggaacggga cccgcatgcc
     2101 gcaatcgccg accagcttcg ccgacgaaat cggcatcgcg ctccacaatc tcgccgatgc
     2161 cgcggccggg agcgtcacgc ccaccctcag gatcggcgtt accggcctca gccgcgccgg
     2221 caagaccatc ttcatcaccg cactgatcca caacctcttg gccggcggcc gcctgcccgg
     2281 ctttgccgtg ctcaccgaag gccggttcat tggcgctcgc ctcgccgaag cccccgaccc
     2341 cggcgtcccg cgcttcgctt acgaacaaca tctcgcagcg cttaccggca gcacaccggt
     2401 ctggcccgag agcacgcggc gcatcagcga gctgcggctg accctcaagt tccagtcgga
     2461 acgctggttc gcgggctgga ccgggccatc gacgctcaac ctcgatatcg tcgactaccc
     2521 gggcgagt
//
LOCUS       DASSGX010000584         2373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1627776, whole genome shotgun sequence.
ACCESSION   DASSGX010000584 DASSGX010000000
VERSION     DASSGX010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1627776"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..484
                     /locus_tag="VFI12_10610"
     CDS             <1..484
                     /locus_tag="VFI12_10610"
                     /inference="COORDINATES: protein motif:HMM:NF016064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HET7056905.1"
                     /translation="RKSPVHIASGVGVGLCVVAGWAVTGLAFDELADRPVAPISLTFV
                     RPAGDSLEWLQRYTALGLPGFGVATVIGSILGAFATAVAMKRFAITTFSDKGDTIHVL
                     SGSALMGVGGVLALGCTVGQGITGVSTLAVGSFIAFAAIVTGGVIGMKAFERILMAEA
                     "
     gene            569..1522
                     /locus_tag="VFI12_10615"
     CDS             569..1522
                     /locus_tag="VFI12_10615"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001312.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HET7056906.1"
                     /translation="MQTPAVETIIALATVLRPQSRKKCRDGDEAEFATEHWNMSTSTR
                     AACVIGWPAKHSRSPKMHKWWLGHYGIDGDYRIEEVSPDDLPAFIGDLRGHGYIGANV
                     TIPHKEAAFRLTEPDERARAVGASNTIWYDNGVLRSTNTDVEGFIGALDAGAPGWDRS
                     EGHAVVIGAGGAGRAVVWGLLERGIRRIHVVNRTHARAVELAERYGDRIVPEHWENLK
                     SVLEGAGLVANATSLGLKGNPDLDVDLSGMAAGAVVADINYVPLETKLLTQARRLGLA
                     RADGLDMLLYQAVTGFDLWFGVRPQVTPELRELLARDIPKS"
     gene            complement(1589..1825)
                     /locus_tag="VFI12_10620"
     CDS             complement(1589..1825)
                     /locus_tag="VFI12_10620"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET7056907.1"
                     /translation="MTSPKQFEFTNSQVTGGHCRIDLNTAPEDVLAALSSVGPARIEA
                     LMLRRPFRNWDDVARVPGISQSIIERLKSSGAGL"
     gene            complement(1942..>2373)
                     /locus_tag="VFI12_10625"
     CDS             complement(1942..>2373)
                     /locus_tag="VFI12_10625"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET7056908.1"
                     /translation="LLRDRGIVLNHGITRRAKRQEGRFQRMRPEHKLIAKYIFPGGEL
                     DDIGHTIAAMERAGFEVHDVEAWRLHYARTCRLWCERLTAAREDAIALVGEEKYRIWV
                     AYLAGVSLAFSRGGARIFQTLASKTAKRPAPLPPTRADLYR"
BASE COUNT          376 a          777 c          800 g          420 t
ORIGIN      
        1 ccgcaagtca ccggtacata ttgcttcggg cgtcggcgtt ggcctgtgcg tcgtcgccgg
       61 ctgggcagtg accgggctcg cgttcgacga gctggcggac cggccggttg cgcccatctc
      121 gctgaccttc gtccgtccgg cgggcgactc gctcgaatgg ctgcagcgct acacggccct
      181 cggcttgccc gggttcgggg tcgccaccgt aatcgggtcg attctcggcg ccttcgcgac
      241 cgccgtcgcg atgaaacggt ttgcgatcac gaccttctcc gacaagggcg atacgatcca
      301 cgtcctctcc ggctccgcgc tcatgggcgt cgggggtgtg ctggcgctcg gctgcaccgt
      361 cggccaggga atcacaggcg tctcgacgct ggccgtcggg tccttcatcg ccttcgcggc
      421 catcgtcacc ggcggcgtca tcggcatgaa ggccttcgag cgcattctga tggccgaagc
      481 gtgaggcggc cccgcgtggt ctgatccgca acctcgcgcg caagcagccg tttggaatcg
      541 actgcccggc tcggccgcga atcaccgggt gcagacaccg gccgtcgaga ccataatagc
      601 cctcgcgacg gtgcttcgtc cgcaatcccg gaagaagtgc cgggatggcg acgaagccga
      661 atttgcgaca gagcactgga acatgagcac ctcaaccaga gccgcatgtg tgatcggctg
      721 gccggccaag cactcccgct cgccgaagat gcataaatgg tggctcggcc attacgggat
      781 cgacggcgac taccgcatcg aggaggtgtc gccggacgac ctcccggcat tcatcggcga
      841 tttgcgtgga cacggctata tcggcgccaa cgtcaccatc ccgcacaagg aggcagcgtt
      901 ccggctgacc gagcccgacg agcgcgcccg cgccgtcggc gcctcgaaca cgatttggta
      961 tgacaacggc gtgctgcgct cgaccaatac ggacgtcgag gggttcatcg gggcgctcga
     1021 cgccggcgca ccggggtggg atcgatccga aggccacgcg gtggttatcg gcgcaggcgg
     1081 tgcgggacgg gctgtcgtct ggggcctgct ggagcgcggc atccggcgca tccacgtcgt
     1141 caaccgcacc cacgcgcggg ccgtcgagtt ggccgagcgg tatggcgacc gcatcgttcc
     1201 ggagcattgg gagaacctga agtcggtgct cgagggggca ggtcttgtcg ccaatgccac
     1261 ctctcttggc ctcaagggca atccggatct cgacgtcgat ctctcgggca tggccgccgg
     1321 ggccgtcgtc gcggatatca actacgtgcc gctcgagacg aagctgctca cccaggcgcg
     1381 ccgactcggc ctggcgaggg cggatggcct cgacatgctg ctttaccagg ctgtgaccgg
     1441 cttcgatctg tggttcggag tgcggcctca ggtgacgccg gagctgcgcg agctgctggc
     1501 gcgggacatc ccgaaatcct gaggggcggt tgaggtccga tgtatcggtc gcgggctcgg
     1561 gcgagccgca ggcgcggcag ccagcttatc agaggccggc tccgctgctc ttcagccttt
     1621 cgatgatgct ctgactgatc cctggcacgc gcgcgacatc gtcccagttc cggaagggcc
     1681 gtctcagcat caatgcctcg atccgcgctg gaccgaccga cgaaagtgcg gccagcacat
     1741 cctccggcgc cgtattcaga tcgatccggc aatggccgcc agtgacctgg ctgttcgtga
     1801 actcgaattg tttgggagat gtcatggttg gtctgcccct actaacccaa gacggctccg
     1861 gaacgtacgg gcaggcgaaa ggttcctcgc atgccgggct gctgcgacgg tcggcgcgcg
     1921 tcccggtcat cacgaattgt ttcaacgata aagatcagct cgcgtcggcg gcagaggcgc
     1981 tggccgcttc gccgttttgc ttgccagcgt ctggaaaatt cgtgcacctc cgcgcgaaaa
     2041 cgcgagcgag acgcccgcga ggtaggcaac ccagatgcgg tacttctcct cgccgaccag
     2101 ggcgatggcg tcctcgcgcg cggccgtgag ccgctcgcac cagagccggc aggtgcgcgc
     2161 atagtgtagc cgccaggctt cgacgtcatg cacctcgaag cccgcacgct ccatggcggc
     2221 gatcgtgtgg ccgatatcgt cgagctcgcc gccggggaag atgtatttcg cgatcagctt
     2281 gtgctcgggc cgcatgcgct ggaagcggcc ctcctgtcgt ttcgcccggc gggtgatgcc
     2341 gtggttcagc acgatcccgc gatcgcggag cag
//
LOCUS       DASSGX010000585         3183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_754711, whole genome shotgun sequence.
ACCESSION   DASSGX010000585 DASSGX010000000
VERSION     DASSGX010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3183
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754711"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..920
                     /locus_tag="VFI12_10630"
     CDS             96..920
                     /locus_tag="VFI12_10630"
                     /inference="COORDINATES: protein motif:HMM:NF018290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HET7056909.1"
                     /translation="MARVIAIANQKGGVGKTTTAVNLAASLAVAERRTLLIDGDPQGN
                     ATSGVGIDGRTLERTVYDALIGNAPIADVISRGVQFAHLDVVPATPDLAGAEIELIEH
                     DRRQLAMRDAIAPIRDRYDLVLIDCPPSLGLITINMLAAADAILIPVQCEYYALEGLS
                     QLLNTVHMIQRSVNPALAIDGVLLTMYDARLNLSRQVAADAREYFGPKVFNAVVPRNV
                     RLAEAPSFGKPIIFYDVSSAGAQAYMHVARELLERLERSSDVEIPPLVHSEPAHDA"
     gene            986..1915
                     /locus_tag="VFI12_10635"
     CDS             986..1915
                     /locus_tag="VFI12_10635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HET7056910.1"
                     /translation="MPTDKPRRLGRGLEALLATRPAPAEQSTGEAGTASGRESVQRIP
                     IAEIRPNPYQPRKEFRAEELAELESSLRENGLLQPISVRPVPQGSGYELIAGERRLRA
                     ATRLGWKDIPAVVRDIDDRALLTLALVENLQRADLNPMEEAHGYQRLQTDFSLTQQQV
                     AEVVGKERSTIANILRLLNLPVTVQEQVQAGQITLGHARALLTLGDEAVMVTMAREIV
                     VKGMSVREVEQRVRVQAPATEKHRKAPSEQSASKGSAETKRIADELRRHLQTDVSISV
                     TARDRGNIQIAFYSSDDLERLLDFILGARRERL"
     gene            2014..2730
                     /locus_tag="VFI12_10640"
     CDS             2014..2730
                     /locus_tag="VFI12_10640"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET7056911.1"
                     /translation="MANRTFVLSRRQVRFFRRGMYVLGGLLTVVILSWLYLATQAARV
                     PLLTQRLQTYQHDARRLDTLQTALDHLEGRFQQVQKMLGAGPAQAAAASTAAAAVDSA
                     AGTVPDHWPLPVAGELLGNGDIREDATPSALDIAVPAGTEVRVSGSGVVADVANDVGG
                     GRVVRVTHADGFVSIYSNLTDVRVIKGSRVPAGTVIGLTGGPTGSLPAHLRFEVRRGG
                     ATVNPTTLMKEGPAHGDLQQ"
     gene            2711..3181
                     /locus_tag="VFI12_10645"
     CDS             2711..3181
                     /locus_tag="VFI12_10645"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HET7056912.1"
                     /translation="MAIFNSKDSKFPDEPRSTAQSGSNALSIIAPGTKIDGDIETDGV
                     IRIEGRVEGSIRAARQVLVGRQGSVTGDIITREAVIGGRVEGSINAADRLEVQSTSMI
                     SGDINTRAIAVIEGGTINGTVRISDARDATDAPHLRDSGEHGVEDAIPHLAAAG"
BASE COUNT          631 a          991 c          979 g          582 t
ORIGIN      
        1 caccgcagcg actggcgact ggggcgcgtc atctataaac tcaacgcact ttcgctctcc
       61 ctcctttcat cccctcccac cccaagcgat ttcacgtggc cagggtcatc gccatagcaa
      121 accagaaggg cggcgtcggc aagacgacca ccgctgtcaa tctcgccgcc agtctggccg
      181 tcgcggaacg ccgaacattg ctcattgatg gtgacccgca ggggaacgcc acaagcggtg
      241 tcggcatcga tggtcggacg cttgagcgca cggtgtatga cgcgctgatc gggaatgcgc
      301 cgatagcaga cgtcataagt cgaggcgtcc agttcgcgca tctggatgtg gtgccagcta
      361 cgccagactt ggcgggagcg gagattgagc tgattgagca tgatcgccgg cagctcgcca
      421 tgcgcgatgc gattgcgcca attcgcgacc gctatgacct cgtgctcatt gattgtccgc
      481 cgtctctcgg tcttatcaca atcaacatgc tcgccgccgc cgacgcgatt ctcattccgg
      541 tgcagtgtga gtattacgcg ctcgaagggc tctctcagtt gctcaatacc gtgcacatga
      601 tccagcgcag tgtgaacccg gcgctcgcaa tagatggtgt actgctcacc atgtatgatg
      661 cgcggctcaa cctctcgcgg caggtcgccg cggatgcacg ggagtatttc gggccgaagg
      721 tcttcaacgc cgtcgtgccc cggaatgtcc gccttgccga agcgccaagt ttcggcaaac
      781 caatcatttt ttacgacgtg tcatcggcgg gcgcgcaagc gtacatgcat gtcgcgcggg
      841 agctgctcga acgtctcgag cgctccagtg acgttgagat cccaccgctc gtccactcgg
      901 agcccgcgca tgatgcctaa cggccttcgg acgcccttgc ctcgtggtgt ctttgcacac
      961 gtttccacaa tacgtccccc gagcgatgcc cactgacaag ccacgccggc ttggccgcgg
     1021 gcttgaagca ttgctcgcaa ccaggccggc accagccgaa cagtccacgg gagaggccgg
     1081 cacagcttct ggacgcgaat ccgttcagcg aatcccgatc gccgagatcc gtcccaaccc
     1141 gtaccagccg cgcaaggagt tccgcgcaga agagctggca gagctcgaat ccagcctcag
     1201 ggaaaacggg ctgcttcagc cgatatccgt tcggccagtt ccgcagggat caggatacga
     1261 gctgatagca ggggagcgac gcctccgggc tgccacacgt ctggggtgga aggacattcc
     1321 cgcagtggtg cgcgacattg acgaccgagc gttgctgacg ttggcgctcg tggaaaatct
     1381 gcagcgtgcg gatctcaatc ccatggagga ggcccatggc taccagcgct tgcagacgga
     1441 cttctcgctc acgcaacagc aggtagccga ggttgtggga aaggaaagat cgacaatagc
     1501 taacatattg cggctactta acttgcctgt tacagtccag gaacaggtac aggcgggaca
     1561 gatcacgttg ggacacgcgc gggccttgct gacattgggc gacgaagctg tcatggtcac
     1621 catggcgcga gagattgtgg tgaagggcat gtccgtgcgc gaggtagagc aacgggttcg
     1681 agttcaggcg ccagcaacgg aaaagcaccg taaggcgcct tcggagcagt ccgccagcaa
     1741 gggatcggcc gagaccaaac gcattgccga tgagctgcga cgtcaccttc agactgacgt
     1801 ctccatttcc gtgacagcca gggaccgcgg caatatccag atcgcgttct actcgagtga
     1861 cgatctcgag cgactcctcg acttcatact cggcgcgcgc cgcgagcggc tgtaatgaga
     1921 cgctctggaa acgcggcccc cgagcgcggc tatggcaaca tctacacgcc gcatgccgga
     1981 tccatgatca ttcatgtcca gcgggaaggt ggtttggcga accgcacgtt cgtgctgtcg
     2041 cgccgccagg ttcggttctt ccggcgcggg atgtacgtct tgggcggtct gctcactgtc
     2101 gtgatactga gctggctcta tctggccact caggccgcgc gcgtcccact tcttactcag
     2161 cgactgcaga cctaccaaca cgacgctcgt cgtctcgata ccctccagac agcgctcgat
     2221 catctcgaag gtcgcttcca acaagtccag aagatgcttg gagcggggcc tgcgcaggcc
     2281 gctgccgcaa gcacggccgc agccgcggtg gattcagccg ccggcaccgt ccccgatcac
     2341 tggccgctcc ctgtcgccgg cgaactgctg ggtaatggcg acattcgcga agatgctacc
     2401 ccatcggcgc tcgacatcgc ggtgccggct ggcacagagg ttcgtgtatc aggatccggt
     2461 gtcgtggccg atgtggcgaa tgacgttgga ggggggagag tcgtcagagt aacgcacgcg
     2521 gacggcttcg tcagcattta ctcgaatctg accgacgtcc gcgtcatcaa gggaagccga
     2581 gtacctgcag gcacggtcat cggactcacc ggcggaccaa ccggcagcct ccctgcgcat
     2641 ctgcgattcg aagttcgccg tggtggtgcc acggtgaatc ccaccactct catgaaagaa
     2701 ggaccggcac atggcgatct tcaacagtaa ggactccaag ttcccggacg agccgaggag
     2761 caccgcacag tcgggcagca acgcgctctc catcattgcc ccgggtacca agattgacgg
     2821 cgacatcgag accgatggcg tgattcgcat cgagggtcgc gtcgagggat ccattcgtgc
     2881 ggcgcgccag gtgctcgttg ggcggcaggg ctccgtgacc ggtgacatta tcacgcgcga
     2941 agcggtcatt ggtggtcgcg tggaagggag catcaatgcc gcggaccggc tcgaagtgca
     3001 gagcacgtcg atgatctccg gtgacatcaa cacgcgcgcc atcgcggtga tcgaaggggg
     3061 gaccatcaac ggaacggtcc gcatcagcga tgcgcgtgac gcaaccgatg cgcctcacct
     3121 gcgtgattca ggcgagcacg gtgtcgagga cgccatcccg cacctcgccg ctgctgggtg
     3181 acc
//
LOCUS       DASSGX010000586         2809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_754828, whole genome shotgun sequence.
ACCESSION   DASSGX010000586 DASSGX010000000
VERSION     DASSGX010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754828"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="VFI12_10650"
     CDS             complement(<1..714)
                     /locus_tag="VFI12_10650"
                     /inference="COORDINATES: protein motif:HMM:NF023324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltotransferase domain-containing protein"
                     /protein_id="HET7056913.1"
                     /translation="MAQLQNVRTIAPALPDQVDPPSSILILDVWPQVDSGEFAVKREF
                     GDTLELWADIFMAGTDKLAARIEYWREGSDEVSIAEMSYFDNDRWHGHFHVGEPGRYR
                     YRIVAFPDHMATKRDEIIKKFDAGIDVSLEIQEFDLILTDVADAHPESAEMIASIREQ
                     LARRAGDGPRVAVVSAIETIEALRPYRNIGGESVSIEYQLIVDRVRARYASWYELFPR
                     SAGTVPDQSATFADVENRLP"
     gene            875..1297
                     /locus_tag="VFI12_10655"
     CDS             875..1297
                     /locus_tag="VFI12_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056914.1"
                     /translation="MPPATVASAIDSGHLSADEQAYMEGLRPYLDVLVGEGRALEQLG
                     QSRSRNIVELTVRMDRYRGAAKDIDQYIQHHQAPTGLADFVGQLQQQIADSLQAIDAS
                     VDAIRHFDWEALGGTVDAFSAAVDSIATLAGTPLAASG"
     gene            1325..>2809
                     /gene="glgX"
                     /locus_tag="VFI12_10660"
     CDS             1325..>2809
                     /gene="glgX"
                     /locus_tag="VFI12_10660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HET7056915.1"
                     /translation="MSNIFGARWEGARTAFGVWSPKAERVELCLFDPQHPARETCRID
                     MTSDKGAWTATVDNAPPGTPYGYRAHGPYKPSAGLRFNPAKLLIDPYARSIAGQVVWS
                     PPVFGYPVGAPDAKKPNGDDSGPDVPRSVVIDPTFDWEGTTRPETPLDETIIYETHVK
                     GLTELHPDIPAGLRGTYAGAAHPAVIDYLRKLGITAIEFLPVHRHVDDSFLLERGLSN
                     YWGYSTLGFFAPDASYAATQEPGGEVAEFKAMVREYHRAGIEVILDVVYNHTAEANHM
                     GPTLSFRGLNNEEFYWLSPDDQSKYFDLTGTGNTVKVHHPSVTSFVLDSLRYWVNEMG
                     VDGFRFDLAPVVGRVEHDFDRHAPIFEAIQDDESLKGIKMIAEPWDVGPGGYQVGGFP
                     DNWSEWNDRYRDLVRSFWRSDDGKMNELGFRLTGSADFYGARSFGPCASINFIAAHDG
                     MTTFDLTAYQEKRNYANGDNNEDGHDNDVNQYIGPDGRTREPEAV"
BASE COUNT          544 a          861 c          889 g          515 t
ORIGIN      
        1 cggcaatcga ttctcgacat cggcgaaggt tgccgactgg tccgggaccg tcccagccga
       61 acgcgggaag agctcgtacc agctcgcata ccgcgctcga acccggtcga cgatcagttg
      121 atactcaatc gagaccgact cgccaccgat attccggtac ggccgcagtg cctcgattgt
      181 ctcgatcgcg ctgaccaccg cgacgcgcgg cccgtctccc gcacggcgcg ccaattgctc
      241 ccggatcgat gcgatcatct cggcggattc gggatgtgcg tcggcgacat cggtcaggat
      301 caggtcgaat tcctggatct ccagtgaaac gtcgattccg gcgtcgaatt ttttgatgat
      361 ctcgtcccgc ttggtagcca tgtggtcggg gaaggcgaca atccgatatc ggtagcggcc
      421 gggctctccg acgtggaaat gaccgtgcca gcggtcgttg tcgaagtagc tcatctcggc
      481 gatggagacc tcgtccgacc cctcgcgcca atactcgatc cgggccgcga gcttgtcggt
      541 gccagccatg aagatatcgg cccagagctc gagggtgtca ccaaattcgc gctttacggc
      601 gaattcgccg gaatcgacct gcggccagac gtcgagaatg agtatgctgg agggtggatc
      661 gacctgatct ggcagcgccg gagcgattgt acgcacgttt tgtagttgag ccatgcagtc
      721 caacgttccc cgcaaacgtc ccaagtccct gagagcatac cgagccgcac ggcccgcaac
      781 aagacgccgg cgattgctcc tgatcgtccc gctactcatc gtcgtagtcg cgataggcgt
      841 tctcaacgcg cctgagagcg tggagaatcg ggtagtgccg cccgcgaccg tggcgtccgc
      901 aatcgacagt gggcaccttt cggcagatga gcaagcgtac atggagggac tccggccgta
      961 cctcgatgtc ctggtcggcg agggtcgagc gcttgagcaa ctcgggcaat cccggagtcg
     1021 aaacatcgtc gagctgacgg tcaggatgga tcggtatcgc ggcgcggcca aggatattga
     1081 tcagtacatt cagcatcatc aagcaccgac agggctggcg gatttcgtag gacaattgca
     1141 gcaacagatc gcggactcgt tgcaggccat cgatgcctct gtggacgcga tcagacattt
     1201 tgactgggaa gcgctcggcg gcactgtcga cgccttctcc gcggcggtcg actcgatcgc
     1261 cactttggcc ggcacaccgc tggccgcatc tggctgatcg gcgaaatctg gagcaagaac
     1321 gacgttgtcc aatatttttg gggcccgctg ggagggcgcc cggacggcat ttggtgtctg
     1381 gtcacccaag gcggagaggg tcgagctgtg tctcttcgac ccgcagcacc ctgcgcggga
     1441 gacctgccgg atcgacatga cctccgacaa gggggcctgg acggcgacgg tggacaacgc
     1501 gcctcccggc acgccgtatg gatatcgggc gcacggaccg tacaagccgt cggccggttt
     1561 gcgcttcaat ccggccaagt tgctgatcga tccgtatgcg cgctcaatcg ccggccaggt
     1621 tgtctggagt ccgccggtgt tcggctaccc ggtcggcgcg ccggacgcga agaagccgaa
     1681 tggtgacgac tcgggtccgg acgtgccgcg gtcggtcgtc atcgatccca ccttcgactg
     1741 ggaggggaca acccggccgg agacgccgct ggacgaaacg atcatctatg aaacgcacgt
     1801 gaagggcctg acggagctgc atccggacat tccggcaggt ctgcgcggca cctatgccgg
     1861 tgcggcgcac ccggcggtca tcgattatct caggaagctg ggcatcactg cgatcgagtt
     1921 tcttccggtg caccggcatg tcgacgacag cttcctcctc gagcgcggac tgagcaatta
     1981 ctggggatac agcacgctcg gtttcttcgc gccagatgcc agctacgccg caacgcagga
     2041 gccgggcggg gaagtcgccg agttcaaggc gatggtccgc gagtatcacc gggcaggcat
     2101 cgaggtcatt ctggacgtcg tttacaacca cacggccgag gcaaaccaca tggggccgac
     2161 gctctcgttt cgcggtctca acaacgaaga gttctactgg ctgtcgcccg acgaccagtc
     2221 gaaatacttc gacctgacgg gaaccggcaa cacggtcaaa gtccatcacc cgtcggtgac
     2281 cagcttcgtc ctcgattcac ttcgctactg ggtgaacgag atgggcgtcg atggcttccg
     2341 gttcgacctt gcgccggtgg tcgggcgggt cgagcatgac ttcgatcgtc atgcgccgat
     2401 cttcgaggcg atccaggacg acgaatccct taaggggatc aagatgatcg ccgagccgtg
     2461 ggacgtcggc ccgggtgggt accaggtcgg tggcttcccg gacaactggt cggagtggaa
     2521 cgatcgctac cgggatctgg tccgttcatt ctggcgatcc gacgatggca agatgaacga
     2581 actgggcttc cgcctgaccg gctccgctga cttctacggc gcccgatcgt tcggcccctg
     2641 cgcaagcatc aacttcattg ccgcgcacga cggcatgacg acgtttgacc tgaccgctta
     2701 ccaggaaaag cggaactacg cgaacggcga caacaacgag gacggccacg acaacgacgt
     2761 caatcagtac atcgggccgg atggacgcac cagagagccc gaggccgtc
//
LOCUS       DASSGX010000587         3693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1519390, whole genome shotgun sequence.
ACCESSION   DASSGX010000587 DASSGX010000000
VERSION     DASSGX010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1519390"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..2063
                     /locus_tag="VFI12_10665"
     CDS             129..2063
                     /locus_tag="VFI12_10665"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HET7056916.1"
                     /translation="MTKTTAPYGSWTSPITSDLIVSSTIGLGDVVVSGNNIYWIESRP
                     SERGRRSIQRGSPDGTIAELTPPDFNVRTRVHEYGGAAYEVVDDTIFFTNYADQLMYR
                     QDPGQAPRPITTSGIFYGDFRYDPKRNRLVSIQEDHGISDIEAINTLVSLNPNGPNAD
                     GGAMLVSGADFVANPRLSPDGRQMAWVEWSHPNMPWDAAQLWIGDVDPEGAIHNGRPI
                     AGGNGSSVFQPEWTPAGDLIFVDARTGWWNFYRYRNGEIEHLLDMEAEFGRPLWVVGM
                     ATYVVISDERLICAYSQNGFWHIGDLNLATGPLTPVPTPFTQIDSIAAEGDIVAFVAG
                     SSTQPTGIYRHDLTTGETTLIKSASSITIDPAYLSEPSLITYPTSDNQVAHALLYLPK
                     NADVDGPEGELPPLIVSSHGGPTSAANTGMELRFQFWTSRGFAVLDVDYRGSTGYGRP
                     YRDTLQDTWGIYDVDDCVYGAKYLVQRGLVDGDRLAVRGGSASGYLTLAALTFRDTFD
                     AGCSLYGISDLEIMALETHKFESRYLDGLIAPYPEGIEIYKERSPLYHIDQINTPLIL
                     FHGLEDKVVPPNQSEMIYEALKANGVPVAIVLYQGEQHGFRQAANIKRTLDGELYFYG
                     KIFGFEPADQIEPIPIANLD"
     gene            2060..2989
                     /locus_tag="VFI12_10670"
     CDS             2060..2989
                     /locus_tag="VFI12_10670"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET7056917.1"
                     /translation="MSAGASGKARAIGATGSLKRLEGETVAYLALITTAVIWGTTAIG
                     TKVALETYQPFVLSAGRWLISLGILLPAMHRFGVKPILDRRTFVLGLFGILGFNAFYT
                     LGLERTTAANGALLNGALPVVVAFLSFLWIGERLAPVRIIGIVISMIGVIGTVLGATL
                     DASVLGNVLVFLAVLSWAIYTIYNRERMQGENTMGIIAGSALFGVAMMIPLAAVEWVQ
                     ETPEAPSLKLIAIILYLSMGPALAANYLWVFALTRVPASQAAVFSNLTPIVGIALAGL
                     VLDEPITRYHVFGSILVIFGVLLTTRRRRPSKT"
     gene            complement(3259..>3693)
                     /locus_tag="VFI12_10675"
     CDS             complement(3259..>3693)
                     /locus_tag="VFI12_10675"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HET7056918.1"
                     /translation="GEISSVQDTGTQFNKVGGSDLVQADGYTWINIMNLGGVRGWMAT
                     DFLSPIADMPCGDGACYPAELDAFFGATGAIVTDGPLNLRSEPGTNSPILMTLEDGDF
                     LTIESVIDSNPYEADGYLWVQVTPAGTNLTGFVAIDFVTPAE"
BASE COUNT          673 a         1210 c         1157 g          653 t
ORIGIN      
        1 ccgggcaatc gaagaaaaat gtcgcagggg agacaggatc atgcagtcgc tctcatgggt
       61 tggacagtgg cccgtatcct tcccattgta cgggctatcc gaccggtagg tcattgcgga
      121 ggcaattggt gacgaaaacg actgcgcctt atggatcctg gacgtcgccg attacatcgg
      181 acctgatcgt ttcgagcacg atcgggctgg gcgacgttgt tgtatcgggc aacaacattt
      241 actggatcga atcccggccc tcagaacgag gccgacgctc gatccagcgc ggatcgccgg
      301 acgggacgat cgcggaactg acgccgcccg atttcaacgt ccgcactcgg gttcacgagt
      361 acgggggcgc ggcctacgag gtcgtggacg acacgatctt cttcacgaac tacgcggacc
      421 agctcatgta ccggcaggat cccgggcaag cgccacggcc gatcaccacc agcggcatct
      481 tctacggcga cttccggtac gacccgaagc gcaaccgcct ggtcagcatc caggaagacc
      541 acggcatctc cgacatcgag gcgatcaaca cgctcgtctc gctcaacccg aacgggccaa
      601 atgccgatgg tggcgcgatg ctggtcagcg gggccgattt cgttgccaat ccccgcctca
      661 gcccggacgg ccggcagatg gcgtgggtcg agtggagtca cccgaacatg ccatgggacg
      721 cggcccagct ctggatcggc gatgtcgatc ccgagggcgc gatccacaat ggccggccga
      781 tcgcgggtgg gaatggatca tccgtctttc agccggagtg gacaccagcc ggcgatctga
      841 tcttcgtcga cgcccggacc ggctggtgga acttctaccg ctatcgcaac ggcgagatcg
      901 agcacctgct cgacatggag gccgagttcg ggcgaccgct gtgggtcgtc ggcatggcga
      961 cctacgtggt gatctccgac gagcgactga tctgcgcata cagccagaac ggcttctggc
     1021 acatcggcga tctgaacctg gcgaccggcc ccctgacgcc ggttccgacc ccgtttaccc
     1081 agatcgattc gatcgccgcc gagggggaca tcgtcgcctt cgtagccgga tcgtcgacgc
     1141 aaccgacagg gatctaccgg cacgatttga ccaccggtga gacgaccctg atcaaatcgg
     1201 ccagttcgat cacgattgac ccggcctacc tgagcgagcc gagcctcatc acctatccga
     1261 ccagcgacaa ccaggttgcc catgccctgc tctacctgcc gaagaacgcc gacgtcgacg
     1321 ggccggaggg tgagctgccg ccgctgatcg tcagcagcca cggcggcccg accagcgcgg
     1381 ccaataccgg gatggagttg cgctttcagt tctggaccag ccgcggattc gccgtcctcg
     1441 acgtcgatta ccggggcagc actggctacg gccggcccta ccgcgacacc ctccaggaca
     1501 cctggggcat ctacgacgtg gacgactgcg tctacggcgc aaagtacctc gtccagcggg
     1561 ggttggtcga cggcgaccgc ctggccgtgc ggggcggcag cgccagcggt tacctgaccc
     1621 tggccgcgct gaccttccgc gacacgttcg atgccggctg ctccctctac ggtatcagcg
     1681 acctcgagat catggccctc gagacgcata agttcgagtc ccgctacctc gacgggctga
     1741 tcgcgccgta tccggagggg atcgagatct acaaggagcg ctcgccgctc taccacatcg
     1801 accagatcaa cacgccgctg atcctcttcc acgggctcga ggacaaggtt gtgccgccga
     1861 atcagtcgga gatgatctac gaggcactca aggcgaacgg cgtgccggtc gcgatcgtcc
     1921 tctatcaagg cgagcagcac ggtttccggc aggcagcaaa catcaagcgg acgctcgacg
     1981 gcgaactcta cttctacggc aagattttcg gcttcgaacc ggccgaccaa atcgagccga
     2041 tcccgatcgc gaacctcgat tgagcgcagg ggcatctgga aaggcgcgcg ctatcggggc
     2101 cactggcagc ctgaagcggc tcgaagggga gacggtcgcc tacctggcgc tgatcacgac
     2161 ggccgtcatt tggggcacga cggcgatcgg caccaaggtt gcgctcgaga cctaccagcc
     2221 gttcgtcctg tcggccggtc ggtggctgat ttcgctcggc atcctgctgc cggcgatgca
     2281 ccggttcgga gtcaagccga ttctggaccg gcgaacgttc gtgctcggct tgttcggcat
     2341 cctcggcttc aacgccttct acacgctcgg cctcgagcgg acgaccgccg ccaacggcgc
     2401 gctgctcaac ggcgcgctcc cggtggtcgt cgctttcctc tcgttcctct ggatcgggga
     2461 gcggctggcg ccggtccgga tcatcggcat cgtgatctcg atgatcggcg tgatcgggac
     2521 cgtactcggc gccaccctcg atgcctcggt cctcggcaac gtgctggtct tcctggccgt
     2581 gctctcgtgg gcgatctaca cgatctataa ccgggagcgc atgcagggcg agaacacgat
     2641 gggcatcatt gccggctccg ccctcttcgg cgtggcgatg atgattccgc tcgcggcggt
     2701 cgagtgggtg caggagacac ccgaagctcc gagtctgaag ctgatcgcga tcatcctcta
     2761 cctgagcatg ggtccggcgc tggccgcgaa ctacctgtgg gtcttcgccc tgacgcgggt
     2821 gccggccagc caggcggcgg tcttctcgaa cctgacgccg atcgtcggca ttgcactggc
     2881 cggtctggtc ctggacgagc cgatcacccg ctaccacgtc ttcggctcga tcctcgtcat
     2941 attcggcgtc ctgctcacca ccaggcggcg ccgcccatca aagacgtaac gggaacgtca
     3001 ttgtggcgcc ggtgcctctg tggccggcgg tggcccgcat ggccacgatg gtttcgccac
     3061 caggcaggct gcggtgccca aaataactcc acggaaaacg atcatcgccc tgcgagcgat
     3121 cgcgggatgc tctctccctg tcagggagag ccggagaggg tatagaggca cccgcgctac
     3181 aatgggcggt cacagacaat caaggcaccg accagagtgg ccggcgcctt gatgtgagcg
     3241 ggatcgtttt tcggccggct attcggccgg ggtgacgaag tcaatggcga cgaagccggt
     3301 cagattcgtt ccggctgggg tgacctggac ccagaggtaa ccgtccgcct cgtacggatt
     3361 cgagtcgatc acgctctcga tcgtgaggaa gtccccgtcc tcgagcgtca tcaggatcgg
     3421 cgagttggtg cccggctcgg accgcaggtt gagtggcccg tccgtcacga tcgctcccgt
     3481 cgccccaaag aaggcatcca gctccgccgg atagcaagcc ccatcgccac acggcatgtc
     3541 cgcgatcggc gagaggaagt cggtcgccat ccagccacgc acaccaccga gattcatgat
     3601 attgatccag gtgtagccgt cagcctgaac gagatcggaa ccgccgacct tgttgaactg
     3661 cgtgccggta tcttggaccg agctgatctc ccc
//
LOCUS       DASSGX010000588         2440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_102659, whole genome shotgun sequence.
ACCESSION   DASSGX010000588 DASSGX010000000
VERSION     DASSGX010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102659"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..976)
                     /locus_tag="VFI12_10680"
     CDS             complement(74..976)
                     /locus_tag="VFI12_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET7056919.1"
                     /translation="MESSAALQPRDGRPASDDLDALSARTRALILESIHHAGAGHIGG
                     PLSATDLLVHLYFRTLNIDPARPDWPERDRFILSKGHSSIALYTVLALRGYLPVEELR
                     TFDAADSRLQGHPDMSILPGLDMSTGSLGQGLSPGVGMALAAKLRGQDYRTWVMLGDG
                     EIQEGQIWEAAFTAARYELDNLTAILDANNLPQFGWPAPGGFTRERPYDDPRAKFEAF
                     GWRVVECDGHDHGSIDTAFQEAIAHTGQPTCVIARTIKGKGISFMEGQYTWHATVPNA
                     DQLATALDELNARLTGDAAGESGR"
     gene            complement(966..1853)
                     /locus_tag="VFI12_10685"
     CDS             complement(966..1853)
                     /locus_tag="VFI12_10685"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HET7056920.1"
                     /translation="MSNLRVGTAPVNWNNSDVPGWRTPTPFPQILDEMAAAGYAGTEH
                     DRQFPDDPAVLKAELAERGLTLCASYQWIRFSPEHGIEQDLAALDGILTLLEALECPV
                     LVVADAMTPERIALAGRVPEDGSASLHKAGWQRLAANLGRVAEKASARGIRTCYHNHV
                     GSQVESPAECARLVELLPETGATLCFDTGHYAYGGGDPTTFVQQHSNLIGHLHLKDVD
                     PAMLERARQSGWSFVDALRQIIFCSFGAGMVDVPAIVDSLRAADFDGWIVVEQDTCPG
                     DPTERAAANRDYLRRCCGI"
     gene            complement(1850..>2440)
                     /locus_tag="VFI12_10690"
     CDS             complement(1850..>2440)
                     /locus_tag="VFI12_10690"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET7056921.1"
                     /translation="NAGIATVYQDLAMIPLMSISRNFFLGSEPTVGWGPFQRFDFAAA
                     DRITRDELAKMGIRIRSADQAVGTLSGGERQSVAIARAVYFGARVLILDEPTAALGVK
                     EAGVVLRYIAQARARGLAVVFITHNVHHAYPIGDRFTLLNRGTSYGTFSKSEVTREEV
                     VQMMAGGEDLAQLEHELEEFARADGVAAQRRPEVSA"
BASE COUNT          371 a          785 c          895 g          389 t
ORIGIN      
        1 agcgtgttgc cccaggcatc gcgtaacgcg acctgcgggg catcggccag tgagcggacg
       61 tgggtagtgg tcatcatcgg ccgctctccc cggcggcgtc gccggtcagc cgcgcgttga
      121 gctcatcgag ggcggtggca agctggtcgg cattcggcac agtcgcgtgc caggtgtatt
      181 ggccctccat gaaggagatg cctttaccct ttatcgtgcg cgcgatcacg caggtcggtt
      241 gacccgtgtg cgcgatcgcc tcctgaaagg cggtatcgat gctgccgtgg tcgtggccgt
      301 cgcactcgac gacgcgccag ccgaaggctt cgaacttcgc ccggggatcg tcgtacggtc
      361 gctcccgggt gaagccgccg ggcgcgggcc agccgaattg cggcaggttg ttggcgtcaa
      421 ggatggcggt caggttatcc agctcgtagc gggcggcggt gaaggcggcc tcccagatct
      481 ggccctcctg gatctcgccg tcgccgagca tcacccacgt ccggtagtcc tgcccgcgca
      541 gcttcgccgc aagcgccatg ccgacgcccg gcgagagccc ctggccgagc gagccggtcg
      601 acatgtcaag tccgggcagg atggacatat ccgggtggcc ctggaggcgg gaatcggccg
      661 cgtcgaaggt gcgcagctcc tcgaccggga gatacccgcg cagggcgagc acggtgtaga
      721 gcgcgatcga cgagtggccc ttgctcagga tgaagcggtc gcgctcgggc cagtcgggcc
      781 gggccggatc gatgttcagc gtccggaaat agaggtggac gagcaggtcg gtcgccgaga
      841 gcgggccgcc gatgtggccg gcgccggcgt ggtggatcga ttcaaggatc agggcccggg
      901 tgcgggcgct cagagcatcg agatcgtcgc tggcggggcg cccatcgcgg ggttgcaggg
      961 cagcgctaga ttccacagca cctccgaaga taatcgcggt tggcggcagc gcgttcggtc
     1021 gggtcgccgg ggcaggtgtc ctgctccacg acaatccagc catcgaagtc ggccgcccgg
     1081 agcgaatcga cgatggccgg aacatcgacc atcccggcgc cgaaggagca gaagatgatc
     1141 tgccggaggg catcgacgaa gctccagccc gactgccggg cacgctctag catagcggga
     1201 tcgacatcct tgaggtgaag gtggccgatc aggttgctgt gttgctgcac gaacgtggtc
     1261 gggtcgccgc cgccgtaggc gtaatggccg gtgtcgaagc agagggtcgc gccggtctcg
     1321 ggtagcagct cgacgagccg ggcgcactcg gccggagact cgacctgact gccgacgtgg
     1381 ttgtggtagc aagtccggat cccgcgagcc gatgccttct cggcgacccg gcccaggttc
     1441 gccgccaggc gctgccagcc cgccttgtgg aggctcgcac tgccgtcttc cgggacccgg
     1501 ccggccagtg cgatccgttc cggcgtcatc gcatcggcca cgaccaggac cgggcattcc
     1561 agcgcctcca gcagcgtcag gatgccatcg agcgcggcca ggtcctgctc gatcccgtgc
     1621 tcggggctga agcggatcca ctggtaggag gcgcagaggg tcagaccgcg ctcggccagt
     1681 tccgccttga gcacggccgg atcgtccggg aactggcgat cgtgttcggt gccggcgtag
     1741 ccggccgcag ccatctcgtc gaggatctgc gggaagggtg tcggcgtccg ccagcccggc
     1801 acatcgctgt tgttccagtt gaccggggcc gtgccgaccc ggaggttgct catgcgctga
     1861 cctcggggcg gcgctgggcg gcgacgccat cggcccgggc gaactcttcc agctcgtgct
     1921 ccagctgcgc caggtcctcg ccgccggcca tcatctggac gacctcctcg cgcgtgacct
     1981 cggatttgct gaaggtgccg taggaggtgc cgcggttgag cagggtgaag cggtcgccga
     2041 tcgggtaggc gtggtggaca ttgtgggtga tgaagaccac cgccaggccc cgggcccggg
     2101 cttgcgcgat gtagcggagg acgacgcccg cctccttgac gccgagcgcg gctgtcggct
     2161 cgtccaggat cagcacgcgc gcgccgaaat agacggcccg ggcgatcgcg accgattgcc
     2221 gctcgccacc ggagagggtg ccgacggcct gatcggcgct gcggatccgg atgcccatct
     2281 tggccagctc gtcccgcgtg atccggtcgg cagcggcgaa atcgaaccgc tggaacgggc
     2341 cccagccgac cgtgggctcc gagccgagaa agaagttccg ggagatgctc atcagcggga
     2401 tcatcgccag gtcctggtag acggtggcga tgccggcgtt
//
LOCUS       DASSGX010000589         2201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_318256, whole genome shotgun sequence.
ACCESSION   DASSGX010000589 DASSGX010000000
VERSION     DASSGX010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318256"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..269
                     /locus_tag="VFI12_10695"
     CDS             99..269
                     /locus_tag="VFI12_10695"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HET7056922.1"
                     /translation="MDWRDYITVDPLICHGQACIKGTRVMVSQAAVSYAADLARENHP
                     VLCALDNARFRR"
     gene            complement(647..1159)
                     /locus_tag="VFI12_10700"
     CDS             complement(647..1159)
                     /locus_tag="VFI12_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056923.1"
                     /translation="MLHVSVYRFTPKWTAFRNDYCRPCAHERISIRTRTFNVLALMWI
                     PLIPLGFQQRWICSECRLPTRLNTRTRHGYKIAGVIVLVLMSLAMWAMPIAEMPAEDH
                     GWIWGMRIGLPLLLAWSVYHLRTAIREPEYDEAFARVQPYASRKCPFCNVDLLNVPDW
                     HCPTCGLKRM"
     gene            1219..>2201
                     /locus_tag="VFI12_10705"
     CDS             1219..>2201
                     /locus_tag="VFI12_10705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HET7056924.1"
                     /translation="MSEAGGVNEWHQLEVSEVLRRLGVEAARGLSVAEAEARLRESGP
                     NEIQAAARVSPWSVLAEQFKNVLVVVLILAVGMSAALGHTVEAIVIGAILLLSVLLGF
                     IQEYRAERAIEALREMAAPTATVVRDGGAREVPARELVPGDVLLLGAGDRVPADARVV
                     EAANLQAEEAALTGESLPVDKTPGALQGELGLGDRRNLIFAGTVVTYGRGRAVVVATG
                     METELGTIAEMVESVESGPTPLQRNLDRVGRILAVAGLAVVALVVALGLMRGQPFFDI
                     LLFGIALAVAVVPEALPAVVTISLALGVRRMVRRNALVRRLPAVETLGSVSV"
BASE COUNT          328 a          709 c          778 g          386 t
ORIGIN      
        1 catgcagatg ggagggcggt ataggtcacg ccgatgccgc gcgaagatct ccggcttcgg
       61 ttcggctctg tggtaacttc tcccaggagt agacttcgat ggactggcga gactacatca
      121 cggtggaccc gctgatctgc cacggccaag cctgcatcaa gggaacccgc gtcatggtct
      181 cacaggcggc ggtctcctat gccgcggatc tggcgcgtga gaaccatccg gtcctttgtg
      241 ccctcgacaa cgcccgtttt cgacgatgag ccggaccagg acgacgttga cgggggtctc
      301 accgaagctc ggcaggaggc cgaggccggg cgcgggatcc gtacggaaga ggtgcggagg
      361 cgatcaggcc cggcgtgaga tctgacggac ggtgccatcc cgaagtttgc gtcaacctcc
      421 ttcccgtttg acgcaaactt tgcgtcagca gcccgaaagc accttgtttc agacgccaaa
      481 gggggttgct cggccactgc gcacaagttt tctgacgcaa ctcgaccgtg ttctgacgcg
      541 actcggaccc ttttcgttgc gacaattcag ggggcgaggg tcgcccctcg aattgttgcg
      601 gcagaaatcc cggcttttgc gtcaagtgga acggcttcgc gacgtgctac atcctcttca
      661 acccgcatgt cgggcagtgc cagtcaggga cgttcagcag gtccacgttg cagaacgggc
      721 acttccggga cgcatagggc tgcactctcg cgaaagcttc gtcgtactcc ggctcgcgga
      781 tggcggtccg caggtggtag accgaccagg cgagcagcag aggcaggccg atacgcatcc
      841 cccagatcca gccgtggtcc tcggctggca tctccgcgat cggcatcgcc cacatcgcga
      901 gcgacatgag cacgagcacg atcacccccg cgatcttgta gccgtgccgg gtgcgcgtgt
      961 tcagtcgggt cggcagccgg cactccgagc agatccagcg ctgctggaat ccgagcggga
     1021 tcagggggat ccacatcaag gccagcacgt tgaaggtccg ggtgcggatg gagatccgct
     1081 cgtgggcgca gggccggcag tagtcgttcc ggaacgcggt ccacttcggc gtgaaccggt
     1141 acacgctgac gtgcagcatc ggaggctcct tggtctggaa gaggcttctc tgagtatagt
     1201 gctcccgggg ggagttgcat gtcggaggcg ggcggcgtga acgagtggca tcaacttgag
     1261 gtttccgagg tgctccggcg gctcggggtg gaggcggccc gggggctgag cgttgccgag
     1321 gccgaggctc ggctccggga gagcggtccg aacgagatcc aggcggcggc tcgcgtgtcc
     1381 ccctggtccg ttctcgccga gcagttcaag aacgtcctcg tcgtggtcct gatcctcgcg
     1441 gtcggcatgt cggcggccct cggccatacg gtcgaggcga tcgtgatcgg ggcgatcctg
     1501 ctgctgtcgg tccttctcgg cttcatccag gagtaccgcg ccgagcgggc gatcgaggct
     1561 ctgcgcgaga tggcggctcc cacggccacc gtggtccgcg acggcggggc gcgcgaggtg
     1621 ccggcccgcg agctggtgcc gggcgatgtc cttttactag gggccggcga tcgcgtgccg
     1681 gccgacgccc gggtggtcga ggccgccaat ctccaggccg aggaggcggc tctcaccggc
     1741 gagtcgctgc ccgtggacaa gacccccggc gcgctccagg gcgagctcgg gctcggcgat
     1801 cgccgcaacc tgatctttgc cgggaccgtt gtgacctacg gccgcgggcg cgccgtggtc
     1861 gtcgccaccg gcatggagac cgagctcggc acgatcgccg agatggtgga gtcggtcgag
     1921 agcgggccca cccctctcca gcgcaacctg gaccgggtcg gccgcatcct ggccgtggcc
     1981 gggctcgcgg tcgtggccct ggtcgtggcg ctcggcctga tgcgcggcca gccgttcttc
     2041 gacatccttc tcttcggcat cgcgctcgcg gtcgcggtcg tgcccgaggc gctgcccgcg
     2101 gtggtcacga tctcgctggc gctcggcgtc cggcggatgg tccggcgcaa cgccctggtc
     2161 cgccggctcc ccgcggtcga gacgctcggc agcgtctcgg t
//
LOCUS       DASSGX010000590         4729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1628289, whole genome shotgun sequence.
ACCESSION   DASSGX010000590 DASSGX010000000
VERSION     DASSGX010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4729
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1628289"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2167
                     /gene="secA"
                     /locus_tag="VFI12_10710"
     CDS             <1..2167
                     /gene="secA"
                     /locus_tag="VFI12_10710"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF019156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008564 - protein-exporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HET7056925.1"
                     /translation="HLLGLSIACLQHDEAFIFDPEYVQEDERLKNLRPVERREAYAAD
                     ITYGTNNEFGFDYLRDNMVITADQMVQRELHYAIVDEVDNILIDEARTPLIISGPAEE
                     STDKYSFFAAIVKQLRPTVHYEVDLKHRTATLTEAGIDRVEQLAGIPKGESIYDERHV
                     ELTHYLEQALTAEAVYHLDKDYIVREVEVIIVDEFTGRMMIGRRYSEGLHQAIEAKEG
                     VRVRRQNVTMATITFQNYFRMYEKLAGMTGTAKTEDEEFHRIYSLEVVVIPTNKEMVR
                     DDAADFVFKNEAGKFRAVIRDVEENQKIGRPVLVGTTSVETSERLSNMLKARGIDHEV
                     LNAKHHEREADIVVDAGQPGAVTIATNMAGRGTDIVLGPGVAEAGGLHIIGTERHESR
                     RIDNQLRGRSGRQGDPGSSRFYVSLDDELMRRFGGERIMGLMERLGMEEDAPIENRMI
                     TKSIEGAQTKVEGHNFDIRRHVVQYDDVMNRHREVIYADRRAIVKGEDMSAKIDEFIT
                     GEIEDIVERNSDEKSREINDDAILEAYLALIPNASLKPEEIEGLDEEGIIEALLEDAT
                     RAYEEVEGRFGAEIMRKVERQIMLAVMDRLWVEHLTEMDDLREGVGLQAYGQRDPLVV
                     YKTEGYRMFQQLLDHVQQDVVHTLYRVQPAVAEQPIRTRMTQEVTTNRESDNGNAPAR
                     TRKIGPNDKCFCGSGKKYKFCHGAAAGKRKIAQEVNSPS"
     gene            complement(2143..2769)
                     /locus_tag="VFI12_10715"
     CDS             complement(2143..2769)
                     /locus_tag="VFI12_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056926.1"
                     /translation="MAHVNSESIGAVRDQAAQILQSVIEQATSRAMEQAKGSRRKASR
                     FSRKASSKTPTVRDIAFNAASGAIELWQAARDRAGETMESVQSSVVDSAAEIKHSAQH
                     ATEEAVDRVKASGEDAVDRVKSISHAVGDLPHKAAGSGKSAATSTAKTGRNMFGLALW
                     TSAAGAIIYYAFLNDERREQVRHIAMQAISEARTLLQDLQGQDGEFTS"
     gene            2961..>4729
                     /gene="mnmG"
                     /locus_tag="VFI12_10720"
     CDS             2961..>4729
                     /gene="mnmG"
                     /locus_tag="VFI12_10720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HET7056927.1"
                     /translation="MSQSSDTTRKYDVVVIGAGHAGCEAALAAARTGASVLVVTPNLD
                     RVGFMPCNPSIGGPGKSHIVAEVDALGGAMAEVADATAVQVRRLNTSKGPAVQAVRHQ
                     IDKALYAIVMKQRLEEQPGILLIQDEAIGLEIHEQSVRSVQLRARGVVSCLSVVVTAG
                     TFLRAAMISGENRQTGARAGDSADQQLAANIQALGIATRRFKTGTPPRIDGRTVDLRK
                     LDIQPGDDEPGWLSRNGRLGNLEPVSLPPVALHRSHARMDGRRPQLSCFRTATHPDLH
                     NLIRANLHRAPIFNGLIEGTGPRYCPSIEDKVARFVDKDEHPIFLEPEGWRTNELYVQ
                     GMSTSLPFEVQETALRMIPGLDQVQVTRFGYAVEYDAVDPNSLSPTLESRQISGLFLA
                     GQVNGTSGYEEAAGQGLVAGLNAARSAAHLPLATIPRSLGYIGVMIDDLVSVPFVEPY
                     RMLTARAEYRLSLRTDTADDRFSALARGWGLIDEARAASVANDRAALNDAISAFERTT
                     ANPNGFEDRALQAIGQAAIGKPMSLAALMRRPTLTLADLQGAAPSLVDSIIARLPVHL
                     LGRLDDELKYAAFVTREQREVART"
BASE COUNT          916 a         1498 c         1510 g          805 t
ORIGIN      
        1 tcatctgctc ggcctctcca tcgcttgtct gcagcatgac gaggcattca tcttcgatcc
       61 ggagtatgtg caggaagacg agcgactgaa gaacctgcgg ccggtcgagc gccgcgaggc
      121 gtacgccgcc gacatcacct acggcacgaa caacgaattc gggttcgact acctgcgcga
      181 caacatggtg atcacggccg accagatggt gcagcgggag ctgcactatg cgatcgtcga
      241 cgaggtggac aacatcctca tcgacgaggc gcggacgccg ctgatcatct ccggtccggc
      301 cgaggaatcg accgacaagt actcgttctt cgccgcgatc gtgaagcagt tgcggccgac
      361 cgtccattac gaggtcgatc tcaagcaccg caccgcaaca ctgaccgagg ccggcatcga
      421 ccgggtcgag cagctcgcgg gcatcccgaa gggcgagagc atctacgacg agcgccacgt
      481 cgagctgacc cactacctcg aacaggcgct gaccgcggag gcggtctacc acctcgacaa
      541 ggattacatc gtccgcgagg tcgaggtcat catcgtcgac gagttcaccg gccgcatgat
      601 gatcggccgg cgctacagcg aagggttgca tcaggcgatc gaggccaagg aaggtgtccg
      661 tgtccgtcgc cagaacgtga cgatggcgac aatcactttc cagaactact tccgcatgta
      721 cgagaaactg gccggcatga ccggtacggc gaagaccgag gacgaggagt tccaccggat
      781 ctacagtctc gaggtggtgg tcatcccgac caacaaggag atggttcgcg acgacgcagc
      841 ggacttcgtc ttcaagaacg aggccggcaa gttccgcgcc gtgatccggg atgtcgagga
      901 gaatcaaaag atcggccgac cggtgctggt gggcacgacg tccgtcgaga cctccgagcg
      961 actctcgaac atgctcaagg cgcgcgggat tgatcatgag gtcctgaacg caaagcacca
     1021 cgagcgcgag gccgatatcg tcgtcgacgc gggccagccc ggcgcggtca cgatcgcgac
     1081 caatatggcc ggccgcggta ccgacatcgt gctcggtccc ggcgtcgcgg aggcgggtgg
     1141 cctccatatc atcggcaccg agcgccacga gtcgcgccgg atcgacaacc agcttcgcgg
     1201 ccgctcaggc cggcagggcg atccggggtc atcccgcttc tacgtttcgc tcgacgacga
     1261 gctgatgcgc cgcttcggtg gcgaacggat catgggcctg atggagcgcc tcggcatgga
     1321 agaggacgcg cccatcgaga accgcatgat caccaagtcg atcgaaggcg ctcagacgaa
     1381 ggtcgagggg cataacttcg acattcgacg ccatgttgtc cagtacgacg acgtcatgaa
     1441 tcggcaccgt gaagtcatct acgcggaccg gcgagcgatc gtcaagggcg aagacatgag
     1501 cgccaagatc gacgaattca tcaccggcga gatcgaggac attgtcgagc gcaattccga
     1561 cgaaaagtcc cgcgaaatca atgacgacgc aatcctcgag gcctatctgg cactgatccc
     1621 gaacgcgagt ctgaagccgg aagagatcga gggcctcgac gaagaaggga tcatcgaagc
     1681 gctgctcgaa gacgcgacgc gcgcctacga ggaggtcgag gggcggttcg gtgccgagat
     1741 catgcgcaaa gtagagcggc agatcatgct ggccgtgatg gaccggctct gggtcgagca
     1801 cctgaccgag atggacgatc tgcgcgaggg cgtcggcctg caagcctacg gacagcggga
     1861 tccgctcgtc gtctacaaga ccgagggcta ccggatgttc cagcagctcc tggaccacgt
     1921 ccagcaagat gtcgtgcaca cgctctatcg cgtgcagccg gccgtcgcgg agcagccgat
     1981 ccggacgcgg atgacgcaag aggtgacaac caaccgggaa tcagacaacg gcaatgcgcc
     2041 ggcccgaaca cggaagatcg ggccgaacga taagtgtttc tgcggcagcg gcaagaagta
     2101 caagttctgc cacggcgctg ccgcagggaa gcgaaagatt gctcaggagg tgaattcgcc
     2161 gtcctgaccc tgcaggtcct ggagcagcgt ccgggcttcg ctgatcgcct gcatggcgat
     2221 gtgccgaacc tgttcgcggc gctcgtcatt caggaacgcg tagtagatga tcgcgccggc
     2281 cgcgctcgtc cagagggcaa gcccgaacat gttgcgtccg gtcttcgccg tgcttgtcgc
     2341 ggcggacttg ccgctgccgg ctgccttgtg gggcagatcg ccgaccgcgt ggctgatgga
     2401 cttgacccga tccaccgcat cttcgccgga ggccttgacg cggtctacgg cttcctcggt
     2461 tgcatgctgc gcgctgtgct tgatctccgc cgccgagtcc accacgctgg attggacgga
     2521 ctccatcgtc tcgcccgcac ggtcccgcgc tgcctgccag agctcgatcg cgccactcgc
     2581 ggcgttgaat gcgatgtcgc gtaccgtcgg cgtcttgctg cttgctttcc ggctgaaccg
     2641 gcttgccttt cgccgggatc ccttggcctg ctccatcgcg cgggatgtcg cctgctcgat
     2701 gaccgactgg agtatctgcg cggcctggtc tcgaaccgcg ccgatcgatt cagagttcac
     2761 gtgtgccatt gctctgcgtc cttcacgatc cgaaccatca gtacacggtc aagacagtag
     2821 ccgatgcggc attcccgcat caaatacgcg cgtaacaaag tgatcgggaa gtcatgacta
     2881 tcggagcatg ccgaatagcc gccgagatgg tttgccaacg tacaatggcg cagcgataac
     2941 gcggagtact acgaacgtac atgtcccaat cttcggacac gacaaggaaa tatgacgtcg
     3001 tcgtgatcgg tgccggccat gccgggtgcg aggctgccct ggcggccgcg cgcaccggcg
     3061 ccagcgtcct ggtcgtcacg cccaacctcg accgggtcgg cttcatgccg tgcaatccgt
     3121 cgatcggcgg tccgggcaag agccacatcg tggccgaggt tgatgccctc ggcggggcga
     3181 tggctgaggt ggccgacgcg accgccgtcc aggtgcgccg gctgaatacc agcaaggggc
     3241 cggcggtcca ggctgtccgg caccagatcg acaaggcgct ctacgcaatc gtcatgaaac
     3301 agcggctcga agagcagccg ggaatcctgc tgattcagga tgaggcgatc gggctcgaga
     3361 ttcacgagca gtccgtgcgc tcggttcaac ttcgggctcg cggagttgtc tcctgtctct
     3421 cagttgttgt cacagcgggc acgttcctgc gcgcggccat gatttcgggc gagaatcggc
     3481 agacaggcgc gcgcgccggt gactctgccg atcaacagtt ggcggccaat atccaggcgc
     3541 tgggcatcgc gacccgcagg ttcaagaccg gcacgccccc gcgaatcgat ggccgcacgg
     3601 tcgatctgcg caagctcgac attcagccgg gcgacgacga gccgggctgg ctctcccgga
     3661 atggtcggct cggcaacctc gagccggtca gcctcccacc ggtcgcgctg caccgatctc
     3721 atgcccgaat ggacggccgg cgaccgcagc tttcgtgctt ccggactgcg acacatcccg
     3781 acctgcacaa cctgatccgg gccaacctgc accgcgcgcc aatcttcaac gggctcatcg
     3841 aaggcacggg gccgcgctat tgcccctcga tcgaggacaa ggtcgcccgt ttcgtcgaca
     3901 aggacgagca tccgatcttc cttgaacccg agggatggcg cacaaacgag ctttacgtgc
     3961 agggcatgag cacaagcctc ccgttcgagg tccaggagac ggctttacgc atgatcccgg
     4021 gtctcgatca ggtgcaggtc acgcggttcg gttacgcagt cgagtacgac gcggtcgatc
     4081 caaattcgct gtcgcccacg ctcgaatcga ggcagatcag cgggttgttc ctcgccgggc
     4141 aggtgaacgg gacatccggc tacgaagaag cggcgggcca gggcctggtt gccggcctga
     4201 atgccgcccg gagcgcggcc catttgccgc tggccacgat tccgcgctcg ctcgggtaca
     4261 tcggtgtcat gatcgacgac ctcgtctctg tgccgttcgt tgagccatac cggatgctga
     4321 ccgcgcgcgc ggagtatcgc ctctcgctgc gaacggacac cgccgatgat cgcttcagcg
     4381 ctctggcgcg aggctgggga ctaatcgatg aggcacgagc cgcttcagta gctaatgacc
     4441 gcgctgcctt gaacgatgcg atctcggcgt tcgagcggac gacggccaat ccaaatggtt
     4501 tcgaggatcg cgccttgcag gcgattgggc aggccgcaat cggcaagccg atgtcgctcg
     4561 ccgccttgat gcggcggccg acgctcacgc tggcagatct gcagggtgcc gcgcccagtc
     4621 tggtcgactc gatcatcgcc aggttgcctg tccacctgct cggtcgcctc gacgatgagc
     4681 tcaagtacgc cgctttcgtc acgcgcgagc aacgcgaagt cgcccgcac
//
LOCUS       DASSGX010000591         2141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1080518, whole genome shotgun sequence.
ACCESSION   DASSGX010000591 DASSGX010000000
VERSION     DASSGX010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1080518"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..916
                     /locus_tag="VFI12_10725"
     CDS             47..916
                     /locus_tag="VFI12_10725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056928.1"
                     /translation="MTPEQLGFIETGPNGWAKTVRDSAVIFRSLTDFADFAPIEQLQR
                     EVMGTTDLDIFPASGLIVVPETGGHVLAAYLDGQLAGALYGFGGFHHGTPRVVSDWMG
                     VWPRYRSAGIGAELKTLQAAIASMAGFREVVWTVDPLRAANARLNFEKLGAYSDHYEE
                     NRYGADYAAGLYGGLPTDRLHMNLPLTDRAVEARLTGRIPPRTAGDIAGVPQFSPGIT
                     VDRALINLPSDIDQLVATDLTAAISWRYALRTNIQAALAEGFVIRGFIPATAERGALS
                     AYLIERDEGHASG"
     gene            913..2043
                     /locus_tag="VFI12_10730"
     CDS             913..2043
                     /locus_tag="VFI12_10730"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HET7056929.1"
                     /translation="MTLTIARIDIFPIRLRLHEPFVVSYATQEDIPTVLLRVTTTDGQ
                     TGWGEATPDPNVTGETWHSTIAILEHDLAPAVLGLDARDREGIVRAMDARVDAVPAAK
                     AAIDIAVHDLVAKAAGLPLWALLGGHSKPHLTISRVVSIKAPEQMAADARQHVRDGFE
                     TVKLKVGDPAKWRLDVARVAAVREAIGPEIGIKIDVNQGWKTPGIAIPAIRQILASNP
                     DYIEQPVDQHDIAALAEVRRATGTPIMADEAVHGVREMLRVVELRAADLVNIKLMKTG
                     GLVNAMRVNAIAEAAGIVGQVGTMVESSIASAAGLHFAMAHANIRTVEMGGPLMIADD
                     IGTLRSCYDRNTITLPDAPGLGVELHEAAIEHRAERQITVGR"
BASE COUNT          364 a          756 c          699 g          322 t
ORIGIN      
        1 tgcacacggg ccgctcgcaa caatccactg accctacaat gccgccatga cgccggaaca
       61 gctgggattc atcgaaaccg ggccgaatgg ctgggccaag accgttcgcg atagcgcggt
      121 gatttttcgc tccctgaccg acttcgctga tttcgcgccg atcgagcagc tccagcgcga
      181 ggtgatgggc acgaccgacc tcgacatctt ccccgcctcc ggcctgatcg tcgttcccga
      241 gaccggcggg cacgtcctcg ccgcctacct cgatggccag ctggccggcg cgctctatgg
      301 tttcggcggc ttccatcacg gcacgccccg ggtcgtctcc gactggatgg gcgtctggcc
      361 gcgctaccgc tctgcgggga tcggcgccga gctgaagacg ttgcaggcgg cgatcgcatc
      421 gatggccggg ttccgggagg tcgtctggac ggtcgacccc ctccgggccg ccaacgcccg
      481 gctcaacttc gagaagctcg gggcctactc cgaccactat gaggagaatc gctacggcgc
      541 ggactacgcg gccgggctct acggcggcct gccgaccgac cgcctgcaca tgaacctgcc
      601 cctgaccgac cgggccgtcg aagcccgcct gaccggccgg attccgccgc gcacagccgg
      661 ggacatcgcc ggcgtgccgc agttctcgcc gggaatcacg gtcgaccggg cactgatcaa
      721 cctgccctcg gatatcgacc agctggttgc gaccgacctg actgctgcca tctcctggcg
      781 atacgccctc cgcacgaaca tccaggcagc gctggccgag ggcttcgtga ttcgcggctt
      841 catccccgcc accgccgagc ggggcgcgct ttcggcctac ctgatcgaac gagacgaagg
      901 acacgcatcc ggatgaccct gaccatcgcg cgcatcgaca tctttccgat ccggctccgg
      961 ctgcacgagc cgttcgtcgt ctcctacgcg acgcaagagg acatcccgac ggtcctgttg
     1021 cgggtcacga cgaccgacgg ccagaccggc tggggcgagg ccacgcccga cccgaacgtg
     1081 accggcgaga cctggcacag tacgatcgcc atcctcgagc acgacctcgc cccggcggtg
     1141 cttgggctcg acgcgcggga tcgcgagggg atcgtccggg cgatggacgc gcgggtcgac
     1201 gcggtcccgg cggccaaggc ggcgatcgac atcgctgtgc acgacctcgt cgccaaggcg
     1261 gccggtctgc ccctctgggc cctgctcggc ggccacagca agccgcacct gaccatttcc
     1321 cgcgtcgtca gcatcaaggc gccggagcag atggccgccg acgcccggca gcacgtgcgg
     1381 gacggtttcg agacggtcaa gctcaaggtc ggcgacccgg ccaaatggcg cctcgatgtc
     1441 gcccgggtcg cggccgtgcg ggaggcgatc ggtcccgaga tcgggatcaa gatcgacgtc
     1501 aatcagggct ggaagacgcc cggcattgcc atcccggcga tccgccagat cctcgccagc
     1561 aatcccgatt acatcgagca gccggtcgat cagcatgaca tcgccgcgct ggccgaggtt
     1621 cggcgggcga ccggcacgcc gatcatggcc gatgaggcgg tccacggcgt gcgcgagatg
     1681 ctgcgggtcg tcgagttgcg ggcggccgac ctggtcaaca tcaagctgat gaagaccggc
     1741 ggcttggtca acgcgatgcg tgtgaacgcg atcgcggagg cggcggggat cgtcggccag
     1801 gtcgggacga tggtcgagag ctcgatcgct tcagcggccg gcctgcactt cgcaatggcc
     1861 cacgccaata tcaggacggt cgaaatgggc ggtcccctca tgatcgccga cgatatcggc
     1921 accctccgaa gctgctacga ccgcaacacg atcaccctcc ccgacgcgcc gggattaggc
     1981 gtggaactgc acgaagccgc aatcgagcac cgcgccgaac ggcagattac cgtcggcagg
     2041 tgagcccagc attccgggat acccggggcc ggccaacgtc ccctttgggg gaaaggcacg
     2101 tcagcttctg atacactacc cccgcagggt accagattgg t
//
LOCUS       DASSGX010000592         2935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_975275, whole genome shotgun sequence.
ACCESSION   DASSGX010000592 DASSGX010000000
VERSION     DASSGX010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975275"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(310..1014)
                     /locus_tag="VFI12_10735"
     CDS             complement(310..1014)
                     /locus_tag="VFI12_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HET7056930.1"
                     /translation="MFFDPMYFVLLAPGLLLMLWAQSRVKSAYRKWGQVRNMRGLTGA
                     QAARMVLDANGLQDVPIEAVPGDLTDHYDPRTRVLRLSQGVYGVPSVAAVAVAAHEAG
                     HAIQHATGYAPLQFRTSIVPVVSIGSNFGFLVLIAGIMFQSVGIAWVGVGLFALSTLF
                     ALVTLPVEFDASHRAKAALVRAGLVDSGVAGGEESKGVNAVLSAAAWTYIAGFATSVL
                     TLLYYVMAVMGMRRSD"
     gene            1352..1750
                     /locus_tag="VFI12_10740"
     CDS             1352..1750
                     /locus_tag="VFI12_10740"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF004036.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-R-sulfoxide reductase"
                     /protein_id="HET7056931.1"
                     /translation="MAYNTLTPDEERVIIHKGTERPFSGEYEELEDVGTFVCRRCNTP
                     LYASVHKFDARCGWPSFDTAVDGAVRESLDADGRRIEITCTNCGGHLGHVFRGEGFTP
                     RNTRHCVNSISMKFIPEGEPLPSAIKGEVL"
     gene            complement(1862..2632)
                     /locus_tag="VFI12_10745"
     CDS             complement(1862..2632)
                     /locus_tag="VFI12_10745"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET7056932.1"
                     /translation="MTETGTPVRVAPIGSLDAIVSSLAPEAYYQSPGGRTFLIAASML
                     GGIARGASVLDVSCGIGQAAVDLAEAFGCKVTAFDNFPPYLAFGRQLAVNRNVSKLVS
                     FMELDGDGVREQFEPGSFDVVLGMGGGLGDVLSGGIAAGLAAAFRWLKPGGLLICGDL
                     ISPAHVSPLMEIVFEQTLTDERSYLTEVRGAGFAPIFVSRATSADWDVMRGTIGRLRE
                     RNLVVGTEDEQRRLRLTEAAQDHPEIAYLNLLARKQKA"
     gene            complement(2636..>2935)
                     /locus_tag="VFI12_10750"
     CDS             complement(2636..>2935)
                     /locus_tag="VFI12_10750"
                     /inference="COORDINATES: protein motif:HMM:NF012945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056933.1"
                     /translation="RIADYDGVMAERQLKAAAGGATLKYVASVPADGPILVGVEAVPT
                     STVLGALQGPENIISIRTTRYDRYPLTISGPGAGAAVTAAGMIADMLELAQAEQR"
BASE COUNT          556 a          963 c          958 g          458 t
ORIGIN      
        1 aacaaccgcg tggccgagct cgtgcaggac aatcgagagg aggaagagcg cagccgtgaa
       61 gaggccgagc agccaggcgg aatcgcgctc gccgggtcga atctcatgcc ggaagacgtt
      121 gactgccaag gtataggcga gcaggccgaa cacggggaac aacgaccagt gcaagcggat
      181 cggtataccc cggatgactg ccaggggaac gcctggctgc gcgtctcgtg ttctcaatag
      241 gggctttctg tcaggtgaat cgaacgagca tggagcggcg gacggtcccg ccgctcccag
      301 ggaacggtac tagtcgctgc ggcgcatgcc catgacggcc atgacgtagt acagcagtgt
      361 caggaccgag gtcgcgaagc cggcgatata ggtccaggcg gcggcactga gcacggcatt
      421 gacgcccttg ctctcctcgc cgcccgcgac gccgctgtca accagaccgg cgcgcacaag
      481 ggcggccttg gcccggtgcg aggcgtcaaa ctcgaccggt agcgtgacca gtgcgaagag
      541 cgtcgagagg gcgaacaggc cgacgccgac ccaggcgatc ccgactgact ggaacatgat
      601 gcccgctatc aggaccagga agccgaagtt cgagccgatg ctgaccaccg gcacgatcga
      661 cgtccgaaac tggagcgggg cgtagccagt cgcatgttga atagcgtgcc cggcctcgtg
      721 ggccgcgacc gccaccgccg ccacggacgg cacgccgtag acgccctgtg agaggcgcag
      781 gacccgggtg cgcgggtcgt agtggtcggt cagatcgccc gggacagcct cgatcggcac
      841 atcctgaagc ccgttggcat cgagcaccat ccgggctgcc tgggcgccgg tcaggccgcg
      901 catgttgcgg acctggcccc acttgcgata cgcgctcttg acccgggatt gcgcccagag
      961 catgagcagc aggcccggcg ccagcaagac gaaatacatg gggtcgaaga acattggtcc
     1021 tcccttgtgg ccggcttcgg aatgtcacac ccggccacat ccgtatacgc tggatgcgcc
     1081 agggtctcac cggacaagcc tttcggctcg ctcgcagggc cggcgttggt acgcgtagtg
     1141 acggccgtgc gtccactccg aggatctcgg aggtggggtt actccccctg ataccggtaa
     1201 ctctacactg tagatagcga aaatcaagcc gccgatacgg gcaggtggcg catcccgcgc
     1261 atggcacagg gtagtggcgg gctcttctcg ccacgtgcac tgggcctaca attttgcgat
     1321 gcagatcgtg ggatcaacct ggaggaaatc aatggcgtac aacaccctga cgcccgatga
     1381 agagcgcgtc atcatccata aggggaccga gcgccccttt agcggcgaat atgaggagtt
     1441 ggaggatgtc ggcaccttcg tctgccgacg ctgcaatacc ccgctctacg cgtcggtcca
     1501 caagttcgac gcgcgctgcg gctggccttc ctttgacacg gcggtagacg gggcagtgcg
     1561 cgagagcctt gatgcggacg gccggcggat cgagatcact tgcacgaact gcggcggtca
     1621 cctcggccac gtcttccgcg gcgagggatt cacaccccgc aacacccgcc actgcgtgaa
     1681 ctcgatctca atgaagttca tccccgaagg cgagcccctg ccgagcgcca tcaagggcga
     1741 agtgctctaa cccacacgga aacgcagcct cggactcctg cagtcggtaa cctacgtttc
     1801 cacgcccgtt ggggagagct ggcgccgctt caatgacaca gcgcggtccg agcgggattg
     1861 gtcaggcctt ctgcttgcgg gcgaggaggt tgagataggc gatttcgggg tggtcctggg
     1921 cggcctcggt caggcggagg cgacgctgct cgtcctcggt accgacgacg agattgcgct
     1981 cccgcagtcg cccgatcgtt ccgcgcatga cgtcccagtc ggcagacgtc gcgcggctga
     2041 cgaagatcgg cgcgaatccc gcccctcgta cctcggtcag gtacgaccgc tcgtcagtca
     2101 gcgtctgctc gaaaacgatc tccatcagcg gcgagacgtg cgcgggggag atcaggtcgc
     2161 cgcagatcag caacccgccc ggcttcagcc agcggaacgc cgccgccaga ccggccgcga
     2221 tgccgccgga cagtacatca cccaatccgc cgcccatccc gagcacgaca tcgaacgaac
     2281 ccggctcgaa ctgttcgcga acgccgtcac cgtccagttc catgaacgat acgagcttgc
     2341 tgacgttgcg gttgaccgcg agctgtcgcc cgaaggcgag atatggcggg aagttgtcga
     2401 aggcggtcac cttacaaccg aacgcctcgg ccaggtcgac cgccgcctgc ccgatcccgc
     2461 aggacacatc cagcacggat gcgccgcggg cgatcccgcc gagcatggag gccgcgatca
     2521 ggaaggtgcg accacccggc gactgataat aggcctcggg cgcgagcgaa ctgacgatgg
     2581 cgtcgagtga tccgatcggc gcgacccgca ccggtgtccc tgtctctgtc atctactacc
     2641 tctgctccgc ctgcgcgagc tccaacatgt cggcaatcat cccggcggcg gtcacggccg
     2701 cgccggcgcc agggccggag atggtcaatg gatatcggtc gtagcgggtg gtccggatcg
     2761 agatgatgtt ctccggcccc tgcaacgcgc cgagcacggt gctggtcggg accgcctcga
     2821 ccccgaccag gatcggcccg tctgccggga ccgaggcgac gtacttgagc gtcgcaccgc
     2881 ccgctgccgc cttcaattgc cgttcggcca tgaccccatc gtagtcagcg attcg
//
LOCUS       DASSGX010000593         4583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_647047, whole genome shotgun sequence.
ACCESSION   DASSGX010000593 DASSGX010000000
VERSION     DASSGX010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647047"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="VFI12_10755"
     CDS             complement(<1..437)
                     /locus_tag="VFI12_10755"
                     /inference="COORDINATES: protein motif:HMM:NF025042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HET7056934.1"
                     /translation="MARAERDGVVIDPRPAILEAFDAALAAVQPDVVVRDVIADTSSG
                     SGEAGCVIAQAAHVEAIAIGKAAVAMMAGALDSLGDRLIRGFVITKDGHIDRSLPARI
                     EVHEAAHPVLDERSVESTHRLLEWVGAIPADATVLCLISGGGSA"
     gene            complement(407..1405)
                     /locus_tag="VFI12_10760"
     CDS             complement(407..1405)
                     /locus_tag="VFI12_10760"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET7056935.1"
                     /translation="MSEEFLTDEVDWLDIDTETDGGTTGESLTLYPEREEKNTRLDKF
                     VASHAPGMSRSWLQRLIDDGNVLVDGQVRGRTFKVTPGQVIEVSIPPIEPDELVAEAI
                     SLDVVYEDADIIAINKPAGMVVHPAPGHRSGTLVNALLHYAPDISMAGSQRPGIVHRL
                     DRDTSGLIVVGRTDAGRLALLEQWADRSVVKEYQAIIRGVPEDAEFAIDAPIGRDPAQ
                     RKRMAVIQSGKSAHSHVTIRERASKASLVDVQITTGRTHQIRVHLAYADYPIVGDRVY
                     NRFGGETGGSSPIADRQLLHAGRLVIRNVAGEVIDLRVPMPADMEAAWRELSETAS"
     gene            complement(1398..1940)
                     /gene="lspA"
                     /locus_tag="VFI12_10765"
     CDS             complement(1398..1940)
                     /gene="lspA"
                     /locus_tag="VFI12_10765"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HET7056936.1"
                     /translation="MPFLPASRVATTRPASIVATMNDPRARHRFPAVAIVAGLAVLVV
                     DFVTKTVMTRWLEWGEQRWLIDGWIGLELTRNRGLAFGLGNGSRITTLLVIAGFVALV
                     WFILRSGLVEHPVGAAAFGLAVGGAMGNLIDRIRDGAVTDFFVIGPWPRFNIADSALT
                     LGLIALALIEFRTQGGYQGV"
     gene            1934..3082
                     /gene="murG"
                     /locus_tag="VFI12_10770"
     CDS             1934..3082
                     /gene="murG"
                     /locus_tag="VFI12_10770"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HET7056937.1"
                     /translation="MAPQSPLRLIIAAGGTGGHVLPAVAVVRELRFRGIPLDTEWIGG
                     TEGIEAEVARAEGIPFDAIPTGKLRRYLDVRTARDAINIPRGVIAARRLVRHFRPDIV
                     FSTGGFVSVPTVIASRGRAPILTHEQTTIIGLANKINLRFADELGLSFEDTAASVGKT
                     KARVVVTGNPVRSELLAGDADRGRQHFAFDADLPVLFVTGGARGASPLNQRIKTILPR
                     LLEYAQVLHQTGPASANADFAELSALKQSLPAVLAARYVPTEFVREEIADAYAMADLI
                     VSRAGAGTVAELSALGKAAIMIPLPLSGGGEQIINARALADHDAGVMLMQDDATPDRL
                     LSEIEQLLADQERRVKMAQAAAALGRRDAAARIADELLALAGYPDPTR"
     gene            complement(3072..4448)
                     /gene="radA"
                     /locus_tag="VFI12_10775"
     CDS             complement(3072..4448)
                     /gene="radA"
                     /locus_tag="VFI12_10775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HET7056938.1"
                     /translation="MAKATAKSTYVCQQCGYVSPTMLGRCPNCRTWDSLVETILPSQS
                     PGGRGARRSTGAVPIRLGDLQTSAIARLPVEIAELNRVLGGGFVPGSLVLLGGDPGIG
                     KSTLVLQAATDIARTGAPVLYVSGEESAEQIKLRADRTALGHDTLLVLTETDIAAVLA
                     AAEQASPALLIIDSIQTMSTPDLESAPGSVSQVRESAARVMAWAKSTGTPVVLIGHVT
                     KEGAIAGPRVLEHLVDAVLYLEGDRYHQFRILRGVKNRFGSTNEIGVFEMDNGGLAEV
                     RHPSEAFVTDRNANAAGSVVTVTLEGTRPILIELQALVAPSALENPRRVVSGVDLNRI
                     HLLAAVLQKRASLSLGSQDIFVNVVGGLRVDEPAIDLALALAIASSLKERPVDLRTVA
                     IGEIGLSGEIRAISQLERRLSEAQRLGYRRAIVPAMLGRRSGDLPSGIDVVRAQTLRE
                     AINAGLSE"
BASE COUNT          807 a         1544 c         1455 g          777 t
ORIGIN      
        1 gctgacccgc ccccggaaat caggcagagc acggtcgcgt cggcagggat cgcaccaacc
       61 cactccagga gtcggtgcgt cgattcgact gatcgctcgt ccagcacggg atgggccgcc
      121 tcgtggacct cgattcgcgc cggcaagctg cggtcgatgt gaccatcctt ggtgatcacg
      181 aaaccgcgga tcaggcggtc tccgagcgaa tccagcgcgc cggccatcat cgcgaccgcc
      241 gccttcccga tcgcgatcgc ctcgacatgc gcagcctgcg cgatcacgca ccctgcctcc
      301 cccgaaccgg aagatgtgtc tgcgatgaca tcgcggacca ccacgtccgg ttggactgcc
      361 gctagtgccg cgtcgaacgc ttcgagaatt gccggccggg gatcgatcac gacgccgtct
      421 cgctcagctc gcgccaagcg gcctccatgt cggccggcat tggtaccctc agatcgatga
      481 cttccccggc aacgtttcga atcacgaggc ggccggcgtg gagcagctgc cggtcggcga
      541 ttgggctgga accgccggtc tctccaccga agcggttgta gacgcgatca ccgacgatcg
      601 gatagtcggc gtaggcgaga tgtacccgga tttgatgcgt ccgccctgtt gtgatctgaa
      661 cgtcgaccag actcgccttt gacgcgcgct cgcgaatggt gacgtgcgaa tgcgcactct
      721 tgccggactg gatgaccgcc attcgcttgc gctgggcagg atcccggcca atgggcgcgt
      781 cgatcgcgaa ctcggcgtct tccggcacgc cgcggatgat cgcctggtac tccttgacta
      841 ccgaacgatc cgcccactgc tccagcagcg cgagtcgccc cgcgtcggtt cggccaacca
      901 cgatcagccc ggatgtgtcc cggtccaggc gatggacaat gcccggccgc tgcgatccag
      961 ccatcgagat atccggcgcg taatgcaaca gggcgttgac cagggtaccg gaccgatgtc
     1021 cgggcgcggg atgaacgacc atccctgccg gcttattgat cgcgatgata tcggcgtctt
     1081 cgtagacgac atcgagcgag attgcctcgg cgacgagctc gtccggctct attggcggaa
     1141 tcgagacctc gatcacttgc cccggcgtga ccttgaaggt ccgtccgcga acctgcccat
     1201 cgaccaacac attgccatcg tcgatcagtc gctggagcca ggaccgactc atgcccggcg
     1261 catggctcgc cacgaacttg tcgaggcgcg tgttcttctc ttctcgctcg gggtagagcg
     1321 ttaacgactc gccggtcgta ccaccgtcgg tttcggtatc gatgtcgagc cagtcaacct
     1381 cgtcagtcag aaattcctca gacaccctga tatccacctt gtgtacgaaa ttcgatgagc
     1441 gcgagggcaa tcaacccgag cgtcaacgcc gagtccgcaa tgttgaaccg gggccagggg
     1501 ccgatcacga agaaatcggt cacggcaccg tctcggatcc ggtcgatcag gttgcccatg
     1561 gcgccgccta cagccagccc gaaggctgcc gccccgaccg ggtgctcgac caggcccgac
     1621 cgcaggatga accagacgag ggcgacgaac ccggcgatca ccaggagcgt cgtgattcgc
     1681 gatccgtttc cgagcccaaa cgccaacccc cggttccggg taagctcaag cccgatccac
     1741 ccgtcgatga gccagcgctg ctcgccccac tccagccacc gcgtcatgac cgtcttcgtc
     1801 acgaagtcga ccaccagcac ggcaaggccg gcgacgatcg ccacagccgg gaatcgatgc
     1861 cgcgcgcggg gatcgttcat ggtggccact atacttgccg gtcgagttgt cgcgacgcgc
     1921 gaagcaggca ggaatggcac cgcaatctcc tctccgactc attatcgccg ccggtggaac
     1981 gggaggtcac gtgctgccgg ccgtcgccgt cgtccgagaa ctgcggtttc gcgggattcc
     2041 gctcgacacc gaatggatcg gcgggacgga gggcattgaa gcggaggtag ccagggcgga
     2101 agggatcccg ttcgacgcga ttcccaccgg gaagctgcgg cggtacctcg atgtccgaac
     2161 cgcacgggat gcgatcaaca ttccacgagg cgtgatagcc gcccgacggc tcgtgcggca
     2221 tttccgaccg gacatcgtct tcagcaccgg cggcttcgtg agcgtgccga ccgtcatcgc
     2281 cagccgcggc cgggcgccga tcttgaccca cgagcaaacg acgatcatcg gtttggcgaa
     2341 caagatcaac ctgcgcttcg ccgatgaact gggtctgagc tttgaggaca ctgcagcgag
     2401 cgtcggcaag acgaaagcga gggttgtcgt gaccggcaat ccggttcggt ctgagttgct
     2461 cgccggcgat gcggatcgag gcaggcagca ctttgccttc gatgccgacc tccccgtgct
     2521 cttcgtgacg ggcggcgcgc gcggggcgtc gccgctgaac caacgaatca agacgatcct
     2581 gccgaggctc ctcgagtacg cgcaggtgct gcatcagacc ggtcctgcat cggccaatgc
     2641 cgatttcgcg gaattgtccg cgctcaagca atcgcttccg gcagtcttgg cggcccgata
     2701 tgtcccaacc gaattcgtcc gcgaggagat tgccgacgcc tacgccatgg ccgacttgat
     2761 cgtatcgcgc gccggggccg ggaccgtggc tgagctgagc gcgctaggga aggccgcgat
     2821 catgatcccg ctaccgctca gcggcggcgg tgagcagatc atcaacgccc gagcactggc
     2881 cgaccacgat gccggtgtca tgctgatgca ggatgacgcg acgccggatc gcctcctgtc
     2941 tgagatcgaa cagttgctgg ctgaccagga gcgccgggtg aagatggcgc aggctgcggc
     3001 tgcgttaggg cggcgggatg cggccgcgag gatagcggac gagcttctcg cccttgctgg
     3061 atacccggac cctactcgct gagtcccgcg ttgatcgctt cgcgcagcgt ctgggcgcgc
     3121 acgacgtcga ttcccgatgg taggtcgccc gatcgccggc cgagcatcgc cgggacgata
     3181 gctcgccggt agccgagccg ctgggcctcg cttaaccgtc gctccaactg gctgatcgcc
     3241 cggatctcgc cactcagccc gatctcgccg atcgccacgg tgcgaagatc gaccggccgc
     3301 tccttcaagc tggacgcgat cgccaatgcc agggcgaggt cgatcgccgg ctcatcgacc
     3361 cgcaacccac cgacgacgtt gacgaagata tcctggctcc ccagcgaaag gctcgctcgc
     3421 ttctggagca ccgccgcgag caagtggatc cggttcaaat ccaccccgct gaccacccgg
     3481 cgcggattct ctagggccga cggcgcaacc agcgcctgca gttcgatcag gatcgggcgc
     3541 gtcccctcga gcgtgaccgt cacgaccgat ccggccgcgt tcgcgttccg gtcggtaacg
     3601 aaggcctcgg acggatggcg cacctcggcc aggccgccat tgtccatctc gaagacgccg
     3661 atctcgttgg tcgagccgaa ccggttctta accccgcgca ggatgcggaa ctggtggtag
     3721 cgatcgccct cgaggtagag gacggcatcg accaggtgct cgagcacccg cggaccggcg
     3781 atcgcccctt ccttggtgac gtggccgatc aggacgaccg gcgtaccggt cgacttcgcc
     3841 cacgccatga ccctcgccgc cgattcgcga acctgggaga cgctgcccgg cgccgattcg
     3901 aggtccggcg tcgacatggt ctggatcgag tcgataatca ggagggccgg gcttgcctgc
     3961 tccgctgcgg ccaggaccgc cgcgatatcg gtttcggtca ggaccaggag cgtgtcatga
     4021 ccgagcgcgg tccggtcggc ccggagcttg atctgctccg ctgactcctc gccggaaaca
     4081 tagaggacgg gcgcgccggt gcgggcgatg tcggttgccg cctgcaggac gagcgtcgac
     4141 ttaccgatcc cagggtcacc gccaagcaag acaagcgagc ccggcacgaa gccaccgccg
     4201 aggacccgat tcagctcggc gatctcgacc gggagccgcg cgatcgcact cgtctgcaaa
     4261 tccccgagcc gaatcggcac cgcgccggtc gaccgcctcg ccccgcgacc gcctggggac
     4321 tgggatggca aaattgtctc caccagggag tcccaggttc gacagttcgg gcagcggccg
     4381 agcatcgtcg ggctcacgta cccgcactgc tggcagacgt aggtcgattt tgcggtggct
     4441 ttggccatgg gctccccgtt gccggactcg aacgcgctgt accaagttta gtacattcgt
     4501 tcgctgatcg tggaggcccg tccgacgaaa aacggggtcc cggtttggga ccccgtttcg
     4561 gtattcaacg tgcccgggtt agc
//
LOCUS       DASSGX010000594         2095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_210306, whole genome shotgun sequence.
ACCESSION   DASSGX010000594 DASSGX010000000
VERSION     DASSGX010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210306"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VFI12_10780"
     CDS             <1..447
                     /locus_tag="VFI12_10780"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HET7056939.1"
                     /translation="PWTMRTSAELVDAGANPATITDALFRLKPKSTVCLWEHALRKVA
                     WEGKLIWTEVDHKDLSNCGADPSEAEGIVNFLLGTDGSRAAAMLYQTDNGYRVSMRSL
                     SDEVDVAQIASVFGGGGHPRAAGCSVPGGIAERDEFLGRVAELLAE"
     gene            466..1359
                     /gene="truB"
                     /locus_tag="VFI12_10785"
     CDS             466..1359
                     /gene="truB"
                     /locus_tag="VFI12_10785"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HET7056940.1"
                     /translation="MTASLHGILVVDKPAGWTSHDVVGWVRRWAGERKVGHAGTLDPA
                     ATGVLPVALNDGTKVLEFLSDATKAYHAEITFGIETDSADGDGTVTAVRDVSLTDAEL
                     GRALDAFRGEISQRPPAHSAIRIGGKRAYDLARAGLDVEVPERIVTIYDLTVADRTET
                     SISLSIDCSKGTYIRSIARDLGAMLGCGAYLSNLVRTRSGPFTLCDAWTTIELEALDP
                     RDSWALIAEHPDAALPGWPAILLSDAESEEWRHGRPIPVTEPPAHGMARVYDNEGRWR
                     GLAEHDRDRDVWRALRVINPS"
     gene            1356..>2095
                     /locus_tag="VFI12_10790"
     CDS             1356..>2095
                     /locus_tag="VFI12_10790"
                     /inference="COORDINATES: protein motif:HMM:NF018299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HET7056941.1"
                     /translation="MSTKFVDGLADLRLPASLVTIGTFDGVHLGHQLLLSATAARGKD
                     LGIPVVVLTFEPIPVSVLRPDHFAGRICPPAEKRGLIAAQGPDYLVTMTFNLELAQLT
                     PEEFMDAVGAATGIRELWVGEAFALGKGRAGDVEQLTGIGESRGFSVCALERQEDIDG
                     VISSSRIRHAIQLGDVSLANRLLGRPFTVKGEVIRGAQFGRTIGFPTANVVPPEGQVA
                     LADGIYASRALLPGEDFLRDALTYVGNRP"
BASE COUNT          354 a          644 c          716 g          381 t
ORIGIN      
        1 ccgtggacca tgcgaacgtc ggcggagctg gtcgatgccg gcgccaatcc ggccacgatc
       61 acagacgcgc tgttccggct gaagccgaag tcgaccgttt gcctctggga gcacgcgctc
      121 cgcaaggttg cctgggaggg caagctgatc tggaccgagg tcgaccacaa ggacctttcc
      181 aactgcggtg ctgacccgtc tgaggccgaa ggcatcgtca acttcctgct cggcaccgac
      241 ggatcgcggg cagccgccat gctctaccag accgacaacg gctaccgcgt cagcatgcgc
      301 agcctctccg acgaagtcga tgtcgcgcag atcgcgtccg tttttggcgg cggcggccat
      361 ccccgtgcag ccggctgctc agtccccggc ggaatcgccg aacgggacga gttcctgggg
      421 cgcgtcgccg agctcctcgc cgaataacgc ctgggatcga ccggtgtgac agcttccctg
      481 cacggcatcc tcgtagtcga caagccggcc ggctggacga gccacgacgt agttggctgg
      541 gttcgccgat gggccgggga gcgcaaggtt gggcatgccg gcaccctcga tccggccgca
      601 accggtgtgc tcccggtcgc gctcaatgac ggcacgaagg tgctcgaatt cctgagcgac
      661 gccacgaagg cgtatcacgc cgaaatcaca ttcggcatcg aaaccgatag cgcggatggc
      721 gacgggacag taacggcggt gcgtgacgtc tcgcttaccg acgcggagct cgggcgcgcg
      781 ctcgatgcgt ttcgcggaga gatcagccag cgacccccgg cgcactcggc catccggatc
      841 ggcggcaaac gtgcgtacga cctggcccgg gcgggtctgg acgtcgaggt cccggagcgg
      901 atcgtgacga tctacgatct cacggtcgcc gaccggaccg aaacttcaat ctctctctcg
      961 atcgactgct ccaaggggac ctacatccgc tcgatcgcgc gggacctcgg cgcaatgctg
     1021 gggtgtggcg cgtatttgtc gaatcttgtg cggaccagga gcggaccgtt cacattatgt
     1081 gacgcgtgga cgaccatcga gttggaagcg ctcgatccgc gcgattcgtg ggcgttgatt
     1141 gccgagcatc cggacgcggc gttgccgggt tggcctgcga tcctgctgtc ggacgcagaa
     1201 tctgaggagt ggcggcacgg tcgcccgatc ccggtaaccg aaccacctgc gcacggcatg
     1261 gcgcgcgtgt atgacaatga gggacgctgg cgcggtctgg cggagcacga ccgggaccgc
     1321 gatgtctgga gagccctgag agtgatcaac ccatcgtgag tacgaaattc gtcgacggtc
     1381 tggccgattt gcgcctgcct gcttccttgg tcaccatcgg aacgttcgat ggtgttcact
     1441 tggggcacca gctcctgctg tccgcaactg cggcacgcgg gaaggatctg gggatcccgg
     1501 ttgtggtgct cacgttcgag ccgatcccgg tgagcgtgct acggcccgat cactttgccg
     1561 gtcggatttg cccgccggcg gagaaacgcg gactcattgc ggcgcagggg ccggattacc
     1621 tggtcacgat gacgttcaat ctcgagcttg cccagttgac gcccgaggag ttcatggacg
     1681 ccgtgggtgc agcaacagga atccgggagt tgtgggtcgg cgaagcattt gcccttggca
     1741 aggggcgcgc gggcgatgtc gagcagttga ccgggatcgg ggagtcgcgc ggcttttcag
     1801 tctgcgcgct cgagcgtcag gaggatatcg acggcgttat cagcagcagc cgcatacggc
     1861 acgcaattca actcggcgac gtttccctcg cgaaccgatt gctgggccgg ccgttcacgg
     1921 ttaagggtga ggtgatacgc ggggcgcagt tcggacgcac catcggtttt ccgactgcga
     1981 atgtggttcc gccggagggg caagtcgctt tggcggatgg catctacgcc tcgcgcgcgt
     2041 tgttgcccgg cgaggatttc ttgcgggacg cgctgaccta tgttggcaat cgccc
//
LOCUS       DASSGX010000595         2973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_647249, whole genome shotgun sequence.
ACCESSION   DASSGX010000595 DASSGX010000000
VERSION     DASSGX010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647249"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VFI12_10795"
     CDS             <1..438
                     /locus_tag="VFI12_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET7056942.1"
                     /translation="QEPDQSIRAFRDDVMALLPRVEQVTAALIDVRSPQEYSGEVISM
                     MGYPQEGAQRGGHVKGARNVPWGKAANEDGTFKSAEQLRAIYEREGVSQGKDVIAYCR
                     IGERSSHTWFVLTYLLGHKNVRNYDGSWTEWGSMVNVPIEKNV"
     gene            441..857
                     /locus_tag="VFI12_10800"
     CDS             441..857
                     /locus_tag="VFI12_10800"
                     /inference="COORDINATES: protein motif:HMM:NF013738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HET7056943.1"
                     /translation="MDRSEYIEYLLDHYQNPRNKHRMEGADVQLGGGNPGCGDLITIY
                     LKVDENDRITEASFEGEGCTISQAGGSIIAEMLPGMSLDEVKALGTDTMIDEMGEDIV
                     KSRVRCATLALGTAQAAVDQVLSDRIRADLRTRSGE"
     gene            864..1946
                     /locus_tag="VFI12_10805"
     CDS             864..1946
                     /locus_tag="VFI12_10805"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET7056944.1"
                     /translation="MPPFPVIDDRPASPRTRAYGIALIGCGGIVNYAHLPAYRAHNLK
                     IVGCYDLSRDTAVSTATAQGIPRVYDSIDELLIDPEVTIVDIAIHPEAQPDVAVRCVE
                     AGKHVLCQKPLALNPIEARRIVDAASFMGAKAAVNQQMRWSGGIAAAKQIIERGFIGQ
                     WTDFQINVSVKTPWEMWPWLRDSDKLDVMFHSIHYLDAIRYLAGDPISVTSRHTTFPG
                     QPERAETKTVTVLDYESGAQALIAVNHHDESGGVHARFRFLGTEGVIEGDIGLLASYP
                     DGGVDTVRARRSDEPAGEWHEPKLTTLWMPDAFIGPMASLINAINTGGTPLSSVADNL
                     NTIRVVTAAYESMAQGRTVPLAQIPG"
     gene            1951..>2973
                     /locus_tag="VFI12_10810"
     CDS             1951..>2973
                     /locus_tag="VFI12_10810"
                     /inference="COORDINATES: protein motif:HMM:NF022466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HET7056945.1"
                     /translation="MIDGASGPIAEIDLCRAWTRLPRSMPLTTTDGRTVDVIHLGTWT
                     HGFGPDFRDAIISVDNGRTEHGSIELHLRTRGWSEHRHHLDPRYNDVILHIVGRHDVV
                     ETRRADGKLVPTVVLDIQALGLATHEVDWSLVGGTVCAERLAREQPDLIRGIIGRLGD
                     ERMTARSARIESRLTTMTPDAVLFAEILDALGYTANREPMAAICDRLPWANVATIAGL
                     PGDALHRLCAVFLGIGGLLPLSDAEIAHTGLDPERAHALSDVWNQIASDWSIAPISST
                     AWTLARIRPSNHPIRRLVQAAALFAGADLHLSTHLLGPIRDGLDPTDSLIALVDSAGA
                     PALGSDR"
BASE COUNT          529 a         1003 c          947 g          494 t
ORIGIN      
        1 caggagccgg accagtcgat ccgtgccttc cgggacgatg tgatggcgct gctgccccgg
       61 gtcgagcagg tgacggccgc actgatcgat gttcgctcgc cgcaggagta ctccggcgag
      121 gtcatctcga tgatgggtta cccgcaggag ggcgcgcagc ggggcgggca cgtcaagggc
      181 gcgcgaaacg tcccttgggg caaggccgcg aacgaggacg gcaccttcaa gagcgccgag
      241 caattgcgcg caatctacga gcgggaaggc gtgtcccagg gtaaggacgt gatcgcctat
      301 tgccgcatcg gcgagcgctc gagccacacc tggttcgtgc tgacatactt gcttggccac
      361 aagaacgtcc gcaactacga tggctcgtgg accgagtggg gcagcatggt caacgtcccg
      421 atcgagaaga acgtctaggc atggatcgct ccgagtacat cgagtatcta ctcgatcact
      481 accagaaccc gcgcaacaag caccggatgg agggcgccga tgtccagctc ggtggcggca
      541 atcctggctg cggcgacctg atcacgatct acctcaaagt ggatgagaac gaccggatca
      601 cggaggccag cttcgagggt gaaggctgca cgatcagcca ggccggtggc tcgatcatcg
      661 ccgagatgct gcccggaatg tcgctcgacg aggtcaaggc gctcggcacc gacacgatga
      721 tcgatgagat gggcgaggac atcgtcaaga gtcgcgtccg ttgcgccacg ctcgcgctcg
      781 gcaccgccca ggcggccgtt gaccaggttc tgagcgaccg gatccgggcc gatttgcgca
      841 cgcgctccgg cgagtagggt cggatgccgc catttccggt catcgacgac cggcccgcgt
      901 cgccgcgcac ccgcgcctac ggcattgccc tgatcggatg cggcggcatc gtcaactacg
      961 cgcacctgcc cgcctaccga gcacacaacc tcaagattgt cggctgctat gacctgagcc
     1021 gcgatacggc cgtcagcacc gccacggcac agggcatccc ccgcgtctac gactcgatcg
     1081 acgagcttct tatcgacccc gaggtcacga tcgtcgacat cgcgatccac ccggaggctc
     1141 agcccgacgt cgccgtgcgc tgcgtcgaag ccggcaagca tgtcctctgc cagaagccgc
     1201 ttgccctgaa cccgatcgag gcgcgccgga tcgtcgatgc cgcgtcgttc atgggcgcca
     1261 aggcggcggt caatcagcag atgcgctggt cgggcgggat cgccgccgcg aagcagatca
     1321 tcgagcgcgg gttcatcggc caatggaccg atttccagat caacgtctcg gtcaagacgc
     1381 cgtgggagat gtggccgtgg ctgcgtgact ccgacaaact cgacgtgatg ttccactcca
     1441 tccactacct cgacgcgatc cgctatctcg cgggcgatcc gatctccgtc accagccgcc
     1501 acacgacctt tcccggccag ccggagcgcg ctgagaccaa gacggtgacc gtgctcgatt
     1561 acgaatccgg ggcacaggcg ctgatcgcgg tcaatcacca cgacgaatcg ggaggcgtcc
     1621 atgcccggtt ccggttcctc gggacggaag gcgtgatcga gggagatatc gggctgctcg
     1681 cgagctatcc cgacggcggc gtcgataccg tccgggcccg gcggagtgat gagccggccg
     1741 gcgaatggca tgagccgaaa ctgaccacac tctggatgcc ggacgccttc atcggcccga
     1801 tggcctcgct aatcaacgcg atcaacaccg gcggaactcc gctgagcagc gtcgccgaca
     1861 acctgaacac gattcgcgtc gtcacggctg catacgagtc gatggcgcag ggacggaccg
     1921 tcccgctcgc ccagatcccg ggttgacgcg atgattgatg gtgcgtccgg tccgattgcc
     1981 gagatcgacc tctgccgcgc ctggacgcgc ctgcctcgct cgatgccact gacgacgacc
     2041 gatggccgca ccgtcgatgt cattcacctc ggaacctgga cccacggctt cggcccggat
     2101 ttccgcgacg cgatcatctc agtcgataac ggccggacgg agcacggcag catcgagctt
     2161 cacctgcgaa cacggggctg gtccgagcac cgccaccacc tcgatccccg gtacaacgac
     2221 gtcatccttc acatcgttgg ccgccatgac gtcgtcgaaa cccgccgcgc ggatggcaag
     2281 ctcgtcccga ccgtggtgct tgacatccag gcgcttggct tggcgacaca tgaggtcgac
     2341 tggtcgctgg tcggcgggac tgtctgtgcg gagcgactgg cgcgggagca gccggacttg
     2401 atccggggca tcatcgggcg tctcggcgac gaacggatga cggcgcgctc ggcccggatc
     2461 gagtcgcgcc tgacgaccat gacgccggac gccgtgctct ttgccgagat cctggatgcg
     2521 ctcggataca cggcaaaccg cgagccaatg gccgccatct gcgaccgcct gccctgggcg
     2581 aacgttgcaa cgattgcggg cctgcccggc gacgccctcc atcgcctttg tgccgtgttc
     2641 cttggaatcg gcggtctttt gccgctctcc gatgccgaga ttgcgcacac cggacttgac
     2701 ccggagcgcg cacatgcgct gtccgatgtg tggaatcaga tcgcgtcgga ctggagtatt
     2761 gcgccgatct cgtcgactgc ctggaccctg gcccgaatcc gtccgtcgaa ccatccgatc
     2821 cgccggctcg tgcaggcggc agcgctgttc gccggcgccg atctccacct ctccacgcac
     2881 ttgctcggtc ccatccgcga cggactggat cccacggaca gccttattgc gttggtcgat
     2941 tccgccggcg ctcccgcact cggcagcgac cgg
//
LOCUS       DASSGX010000596         5112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_319062, whole genome shotgun sequence.
ACCESSION   DASSGX010000596 DASSGX010000000
VERSION     DASSGX010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5112
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319062"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="VFI12_10815"
     CDS             <1..722
                     /locus_tag="VFI12_10815"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HET7056946.1"
                     /translation="TLEDTMYLVKAEDGQWRWFFGGTKEFVDLAIETFGDKNEAGLKS
                     LGDGDRITNTVKDLDDFYRDAFSYTDLKYETPGVVVVAQGQSEMTACGPAQTGFWAFY
                     CPGDATLYLDEPFLKQLGQQAPFAESFVIAHEWAHHVQTAVGLVRVQNPPTEWNEVFS
                     VELELMADCMAGAWAQDVDSRGLLGVNDIQQTIDFTLQYLGDPPGVGTYDPQAHGTAE
                     QRAQAIMGGYQDGFLACNIQV"
     gene            813..1625
                     /locus_tag="VFI12_10820"
     CDS             813..1625
                     /locus_tag="VFI12_10820"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HET7056947.1"
                     /translation="MKLAIQTSRLPGRSLEEKFANARRYGFEAVEVNIGPEFDLAARF
                     DDVRRASESAGLPVSAICTHSIHDPLMPDPALRERRFAGLAQLLQLADELRAEGVITV
                     PARVDVEFLRLADAEQDLIELAVREFQRWSATLPPGRSAAFLEPLNRYEATLLRRVEQ
                     AADMAARIGSPRIKALADMFHMNIEEADLADPIRAAVSQLAYVHTADNNRLQPGAGCL
                     DFLPTFRALKQIGYEGYISIECSALDGPLAAGGPDAMLPATAAFLRERWDAA"
     gene            complement(1633..2937)
                     /locus_tag="VFI12_10825"
     CDS             complement(1633..2937)
                     /locus_tag="VFI12_10825"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HET7056948.1"
                     /translation="MQGLLAAGLALAVVAIAAFIGLRFTGSSDARLRDTTPTVISAIT
                     PNFAASTPAAAPSPTAVAPVPSPTSAGAPTPRPTDKWGQVIETITEDSSAAMFRGTIG
                     RTGVYPGDPITKRPAILWDDPLLDENFSVPVVQGNRVYVGGNDDALKCFDAETGDVIW
                     SFDAKGDISGSPAIGGGRVFVGSFDGNVYAVDAETGKRIWRFETAEPIHSSPAIDGSI
                     VYIGGYDRVLYALSMLDGTLIWAAPTGDKIQSSPAVSGDLVVVGSDDGYIYGLERLTG
                     KRLWRVKTGAAVTSTPAIVGDTVFAASSNGTVMAIHAADGDVAWEHDAGSKISFSSPA
                     VDANTVVIGTDAGQLLALRADNGKERWTFEAKAEIRSSPIIVGGTVYAGSYDENLYGV
                     DLKTGEAAWSTDRGAAVGSPVFANGTIYVSLNTGELLALRRE"
     gene            3270..3962
                     /locus_tag="VFI12_10830"
     CDS             3270..3962
                     /locus_tag="VFI12_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056949.1"
                     /translation="MLPLLTSMLGTGSAESLSRRARAVSAVSISREQPLLASAGTGQD
                     NPSNALFQVVGTDTPTNTPTNTPVPATDTPTNTPTNTPVPATDTPTNTPTNTPVPATD
                     TPTNTPTNTPVPATDTPTNTPTSTPNGTITSTATSTAIVTNTATATDTATATNTATAT
                     ATATADDSLGGVTATANPTAVGGEPDVTELPNTGGSPIDQTQSGFAWTLMALLIAIGA
                     FAARAGWLRHRR"
     gene            complement(4112..>5112)
                     /locus_tag="VFI12_10835"
     CDS             complement(4112..>5112)
                     /locus_tag="VFI12_10835"
                     /inference="COORDINATES: protein motif:HMM:NF021570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HET7056950.1"
                     /translation="IVAEPAPIATGTATGRLTAAVGPVTVSAAVAVMLAGVWRDGLST
                     CLVGALIIMALAGWTCRNDPGRLLVILFAATALALGLATEQVALKGDIGRMNTVFKLS
                     LQAWMLFGIAAAVGSIVVWTSQKRRSIPARIWTGTLVVLIAGAAVYPIVAVPARTHDR
                     FAPMPTVLDGMAYMNGAVYSDAPDGRPEVRFPLSNDLAAIDWLRANIAGSPVVLEATI
                     PEYRWGSRVSVYTGLPTVLGWNWHETQQRPGFGMMLEERRTDVQRMLGETTSFASIRP
                     LLDKYHVRLIYIGDLERAYYDASALAKFEAAAQDGDLSVIYQGPGVTIYEYLDVPG"
BASE COUNT          915 a         1671 c         1682 g          844 t
ORIGIN      
        1 agacgctcga ggacacgatg tacctggtga aagccgagga tggccagtgg cgctggttct
       61 tcggcggcac gaaggagttc gtcgaccttg cgatcgagac cttcggcgac aagaacgaag
      121 ccggcctgaa gtcgctcggc gacggcgatc gcatcacgaa tacggtcaag gatctcgacg
      181 acttctatcg cgatgctttc agctacaccg acctgaagta cgagacaccg ggcgtggtcg
      241 tcgtcgcgca aggtcagtcg gagatgaccg cctgcggccc cgcccagacc ggcttctggg
      301 ccttctactg cccgggcgac gctacgctct acctcgatga gccgttcctg aagcagctcg
      361 gccagcaggc gccgttcgcc gagtcgttcg tgatcgcgca tgaatgggcg caccatgttc
      421 agacagcggt cggtttggtt cgcgtccaga acccgccgac cgagtggaac gaggtcttca
      481 gcgtcgagct cgagctcatg gccgattgca tggcgggcgc gtgggcgcag gacgtcgact
      541 cgcgggggct gctaggcgtg aacgacatcc agcagacgat cgatttcacc ttgcaatacc
      601 ttggcgatcc gccgggcgtc gggacctacg atccccaagc ccacggcacg gccgagcagc
      661 gagcgcaggc gatcatgggc ggctaccagg acggcttcct cgcctgcaac atccaggtct
      721 gacctgccct gaagtggttc cgcgtgcggt acgatgccgg ccacgttcgg caagtggcgc
      781 gggccgccgc gccactctgg agaggtccgc ttgtgaagct cgcaatccag acgtcgcgcc
      841 tgccgggtag gagcctcgaa gagaagttcg cgaatgcgcg gcggtacgga ttcgaagcgg
      901 ttgaagtcaa catcgggccg gaattcgacc tggccgcacg cttcgatgac gttcgccggg
      961 ccagcgagtc agccgggttg ccggtcagcg cgatctgcac acactccatc catgacccgc
     1021 tcatgcccga tccggctctg cgggaacggc gattcgcggg cctggcgcag ctcctgcaat
     1081 tggctgacga gttgagggcg gagggcgtaa tcaccgtgcc ggcgcgggtg gatgtcgagt
     1141 ttctcaggtt ggcggacgcc gagcaggacc tgatcgaact tgcggtccgg gagttccagc
     1201 gctggagcgc gacgctgcca ccgggacgat cagccgcctt cctcgaaccg cttaatcgct
     1261 acgaggcgac actcctccgc agggttgagc aggccgccga catggcggcg cggatcggct
     1321 ccccacgcat caaggcactg gccgacatgt tccacatgaa tatcgaggaa gcggacctgg
     1381 ccgatccgat ccgcgcggcc gtgtcgcagc tcgcgtatgt ccacaccgcc gacaacaatc
     1441 ggctccagcc gggcgccggt tgtctcgatt tcctcccgac gttccgcgcg ttgaagcaga
     1501 tcgggtatga ggggtacatc agcatcgagt gcagcgccct cgatggtccg ctcgcggcag
     1561 gcggaccgga cgcaatgctg ccggcgaccg ctgcgtttct gcgcgagcgc tgggacgcgg
     1621 cctgagcccg gtctattccc ggcgaagggc cagcagttca ccagtgttga gggagacgta
     1681 gatcgtgccg ttggcgaaga cgggagagcc gacggcggct ccgcgatcgg tcgaccaggc
     1741 cgcctcccct gtcttcaggt cgacgccata caggttctcg tcatagctgc ctgcatagac
     1801 cgtcccgccg acgatgatcg gtgatgaccg gatctcggcc ttggcctcga aggtccagcg
     1861 ctccttaccg ttgtcggccc gcagcgcgag caactgcccg gcgtcggtcc cgatgacgac
     1921 tgtgttcgca tcgacagccg gcgacgagaa ggaaatcttg ctgccagcgt catgctccca
     1981 tgccacgtcg ccgtcggccg cgtggatcgc catgacggtc ccgttgctgc tggcggcaaa
     2041 aaccgtgtcg ccgacgatgg ccggagtcga ggtgacagcg gcgccggtct tgactcgcca
     2101 caaccgtttc ccggtcagtc gctccagccc gtagatgtac ccgtcgtcgc tgccgaccac
     2161 gaccaggtcc ccggaaacgg ccggcgagga ttggatcttg tcgccggtcg gcgcggccca
     2221 gatgagcgtg ccgtcgagca tcgagagggc gtagaggacg cggtcatatc caccgatgta
     2281 gacgatcgag ccgtcgattg ccggcgagga atggatcggc tcggcggtct cgaagcgcca
     2341 gatgcgcttg ccggtttccg cgtcgaccgc gtagacgttg ccatcgaagc taccgacgaa
     2401 gacccgacca ccaccgatgg ccggggaacc cgaaatgtcg cccttcgcgt cgaacgacca
     2461 gatcacgtcg ccggtttccg cgtcgaagca cttcagcgcg tcgtcgttgc cgccgacgta
     2521 gacgcggttg ccctgcacga ccggcacgga gaagttctcg tcgaggagcg ggtcatccca
     2581 gaggatcgcc ggtcgcttcg tgattgggtc gccggggtag acgccggtcc gcccgatcgt
     2641 gccccggaac atggcggcgg acgaatcttc ggtgatcgtc tcgatcacct ggccccactt
     2701 gtcggtcggc cgtggcgtcg gcgcgccggc actggttgga ctcggaacgg gcgccactgc
     2761 ggtcggtgag ggtgctgctg ccggagtcga tgcggcgaag ttcggcgtaa tggcggagat
     2821 aacggttggt gtggtgtcgc gaagcctcgc gtccgaagag cccgtgaagc gcaatccgat
     2881 gaacgcggca atggcaacga cagcgagggc cagcccggcg gcgagaaggc cttgcatgcg
     2941 cacttcaagt tgtcgcctgg tctgcaccga gcgcgtttcc tttggtcctg cgaatcatgc
     3001 ggtggtctga aaccggatgg taccccaact tgcctcatga aggcgcaggg cgatgatggt
     3061 cacggcggga gacgccgcat cgatgccgga cggaggagat gcgcgaagcg gcacgaaacc
     3121 ggcgggattt ccggaattga cggaaggcgt tccctcatgc tagtttgtgc cattccgttg
     3181 tgtctgctac atggggcggg gtggcagtgg gcaagcgtgc gcggttggtg cttctaagtg
     3241 ctgcggttgg tctgggtgtt atcgccctca tgctgccgct cctgacgtcc atgctcggga
     3301 ctggttcggc cgaatccctg tctcgccggg cgcgggctgt ctcggcggtg tccatcagcc
     3361 gggaacaacc gctgcttgcc agcgcgggaa ctggccaaga caatccatct aacgctctgt
     3421 tccaggtcgt aggcacagac acgccgacca acacgccgac gaataccccg gttccagcaa
     3481 cggatacacc aacgaatacc cccaccaaca cgccggtccc ggccactgac acgccgacca
     3541 acacgccgac caatacaccg gtcccggcga ccgatacgcc gacgaacaca ccgacgaaca
     3601 cgcctgttcc ggcgacggac acaccgacga ataccccgac cagcacgccg aatggcacca
     3661 tcaccagtac ggcgaccagt accgcaatcg tcacgaacac ggcaacagcg accgacacgg
     3721 caacagcgac caacacggcg actgccaccg ctacggcgac ggcggatgac tcactcggcg
     3781 gcgtcaccgc gacggccaat ccgactgccg tcggcgggga gccggacgtc acagaacttc
     3841 cgaacactgg tggcagcccc atcgatcaga cccagagtgg gttcgcctgg acgctgatgg
     3901 ctctgctcat cgcgatcggc gcgttcgccg cccgagctgg ctggctccga caccggcgct
     3961 gaaagtcgca tccataccgt gatgtagtcc cggcatcctg cccggctgcc gtcgccgtca
     4021 cgccgggcga ggccgttcgg ggaaacgttg ctgacgaggt cgaaaccgcc tgaacgcgag
     4081 ttggcgatcg acgcacgggc gccggaattc cctacccggg aacgtccagg tactcgtaga
     4141 tcgtcacgcc tggaccctgg tagatgaccg agaggtcgcc atcttgcgcg gcggcctcga
     4201 acttggcgag cgccgaggcg tcgtagtatg cccgctcgag gtcgccgata tagatcaggc
     4261 ggacgtggta cttgtcgagc agtgggcgaa tcgaggcgaa tgatgtcgtt tcgccgagca
     4321 tccgctgcac atcggtgcgc cgttcctcga gcatcatgcc gaagccgggg cgctgctgcg
     4381 tctcatgcca attccagccg aggaccgtcg gcaatccggt atagacgctg acccgcgacc
     4441 cccatcggta ctcgggaatc gtggcctcga gcacgaccgg cgagccggcg atgttcgcgc
     4501 ggagccaatc gatcgcggcc aggtcattcg acagcggaaa tctgacttcc ggccggccgt
     4561 cgggcgcatc gctgtagacc gcgccgttca tgtaggccat gccgtcgagc acggttggca
     4621 tcggcgcgaa gcggtcatgc gtgcgagccg ggacggcgac gatcggatag accgctgcgc
     4681 cggcaatcaa taccacgagc gtgccggtcc aaatccgggc cggaatgctc cgtcgtttct
     4741 ggctcgtcca cacgacaatg ctgccgactg ccgccgcgat cccgaagagc atccacgcct
     4801 gcagcgagag cttgaagacc gtgttcatgc ggccgatatc gcccttgagc gcgacctgct
     4861 ccgtggccag cccgagcgca agcgccgtgg cggcgaacag gatgaccagg aggcggcccg
     4921 gatcgttccg gcacgtccac ccggcgagcg ccatgatgat cagcgcaccg accaggcagg
     4981 tgctcagccc gtcccgccaa accccggcca gcatcacagc cacggcggct gacacggtca
     5041 ccggtccaac tgcggcagtg agccggccgg ttgctgtccc ggtggcgatg ggcgccggtt
     5101 cagcgacgat gc
//
LOCUS       DASSGX010000597         4473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1629069, whole genome shotgun sequence.
ACCESSION   DASSGX010000597 DASSGX010000000
VERSION     DASSGX010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1629069"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..909
                     /locus_tag="VFI12_10840"
     CDS             121..909
                     /locus_tag="VFI12_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056951.1"
                     /translation="MATDLHSRDSVLVVTAATPDPRAALVLNTWLDADRLIGFVQPTL
                     WSDYGMDTARCALDAAYSALLGAQCLADLTVANRLKRAVMAANDAVIDANQSRCGNDI
                     ERRVGVGMALCLRSGRTATMALVPPVQALLFQGGAPTWFPRRESWTGEDRGLSGTPLG
                     WSADPSPTLVSTVAEHADEIVLTSTHVAASLAHSDDLPASAGALCDRIATMADGTDPI
                     ELVALSTRFEPGSLTGSIRATTQYVLTLVDHRARAVWTALCAPA"
     gene            complement(1204..1446)
                     /locus_tag="VFI12_10845"
     CDS             complement(1204..1446)
                     /locus_tag="VFI12_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HET7056952.1"
                     /translation="MTYVITQPCIGTKDASCVEVCPVDCIHSTDEADQYFINPDECID
                     CGVCAEVCPVEAIFFEDDVPEQWANFIPLNKAFFEK"
     gene            complement(1791..3458)
                     /locus_tag="VFI12_10850"
     CDS             complement(1791..3458)
                     /locus_tag="VFI12_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET7056953.1"
                     /translation="MAVSSTVDQSEVAATTQRMTGGDALAEMLIRNGVDTLFGLPGVQ
                     LDGLFNAFWARQDRLRIIHTRHEQATAYMADGYARVTGREGVCVVVPGPGLLNATAAL
                     STAYACNSPVLCVTGQIKSDLIDHGRGLLHEIHDQLGMIRHITKSAERAMTPGEIPGV
                     VSKAFAELRSGRMRPVEIEVPPDTLFAEEDVALLAPSAERTLLVPAAGRLEAAAQYLA
                     GARNPVIYAGGGVIRGRAWDELRQLAELLQAPVVMSANGKGAVSDHSYLAQNQVGELE
                     LRPKADVVLVVGTRFLDNLSQPRPMNPETVILRIDIDPEEIERDITPTVSMVGDAKPA
                     LAALIDLLEQQVGGRPSRKAELEDLKKRVNERIKSILPQADFGRAMRNALPENGVVVS
                     EMTQLGYWSNFAMPVYEPNTYITSGYQGTLGYGFPTALGAKVGVPDAPVLSINGDGGF
                     GFALNELATMAQHTIPAVVLVFNDSAYGNVKRIQQVDLGGREIASSLQNPDYVKLAEA
                     FGVVGRRTETADGLQTAIEESIKANEPTLIEIPVGQMPNPWKALGLR"
     gene            complement(3528..>4473)
                     /locus_tag="VFI12_10855"
     CDS             complement(3528..>4473)
                     /locus_tag="VFI12_10855"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HET7056954.1"
                     /translation="QPHGGNVGEEAIFAGYHQLNGNGEMTGIAWVNESGLLTSAVAIT
                     NTGSVGVVRDALIAYDRQRFTYEREPDFHLPVVGETYDGWLSDINGFHVKPEHLYAAA
                     DAARSGPVAEGCVGGGTGMNCHEFKGGIGTSSRRVSEKHGGWTVGVLVQANYGARHTL
                     RIDGVPIGTEIGFDEVPSHQGAPKEGSIIIILATDAPLLPDQCRRIAQRATIGLARVG
                     GNGNNGSGDLFLAFSTANRGMIAGEPFEPFPVRSLQPDAMTPLFEAAADATEEAIINA
                     LCMATTMTGVNGRTSHAMPLDRVRDLMTKYNRPAPERA"
BASE COUNT          724 a         1527 c         1429 g          793 t
ORIGIN      
        1 ccatgccaat tgactgtacg tacactgggc agtacgctag acctcaggtc acggctgccc
       61 attctcgatc ccgccatgca ctccggaatg ggccattgac cccgcatcgg aggaaccctc
      121 gtggcaaccg atctgcactc ccgggacagc gttctcgtcg tcacggcggc aacgcctgat
      181 ccgcgggccg cgctcgtcct gaatacctgg ctcgacgcgg accggctgat cggcttcgtt
      241 caacccacgc tctggtcgga ctacgggatg gacaccgccc gctgcgcgct cgacgcggcg
      301 tactcggcgc tgctcggcgc gcaatgcctg gctgatctga ccgttgccaa ccggctcaaa
      361 cgcgccgtca tggcggccaa cgacgccgtc atcgacgcga accagtcacg ctgcggcaac
      421 gatatcgagc ggcgcgtcgg cgtcggcatg gcgctctgcc tgcgctcggg ccggaccgcg
      481 acaatggcgc tggtcccacc ggtgcaggcg ctcctgttcc agggcggcgc gccgacctgg
      541 ttcccgcgcc gagaatcctg gaccggggag gatcgcggtc tgagcgggac gcccctcggc
      601 tggtcggccg atccatcgcc gacgcttgtc tcgacggttg ccgagcacgc cgacgagatt
      661 gtgctgacct caacccacgt tgcggcgtcc ctcgcccact cggatgatct accggcgtcg
      721 gccggagccc tttgcgaccg gatcgcgacc atggccgacg ggaccgaccc aatcgagctc
      781 gtcgccctct cgactcgctt cgagccgggg tcactgaccg gcagtatccg cgcgacaacc
      841 cagtacgtcc tcaccctcgt cgaccaccgc gcccgcgccg tctggaccgc cctgtgtgcc
      901 ccggcctaga gcggtccccg gaagagggac cggttcagaa catggagccc cgggcggcct
      961 cgcccgggtt ttggtgtgac acggccggac gagccgcccg gggctccatc aggcaggcac
     1021 tttttctgaa acccatttat aactcgcgcg acgcgacctg aatcgaacat ggattccgca
     1081 ccgtggcgcg ggtgcctctg tgccccgcgc cacggtaaac gcgccagtat caaccatgct
     1141 gcggtaggca agaaaacgcc gggggagtca actcccccgg cgttcttggc tctcgtgaac
     1201 cggctacttc tcgaagaagg ccttgttcaa cgggatgaag tttgcccact gctccgggac
     1261 gtcgtcctcg aagaagatgg cctcgaccgg gcagacttcg gcgcaaacgc cgcagtcgat
     1321 gcactcgtcc gggttgatga agtactggtc cgcctcgtcg gtcgaatgga tgcaatcgac
     1381 cgggcagacc tcgacgcacg aggcgtcctt cgtgccaatg cacggctggg tgatgacgta
     1441 cgtcatcgct cgttcctcct ccccacggcg tgggaagccc aaattgggtg aagaactgcg
     1501 ctgggcccac tgattcgcaa tcaacgctcg ccgtcggcga gcttgcaggc cctttgagta
     1561 taacaacggc gcccggcctg ccgtcattgt gcccgctgca cgatcaaagg gaattgtttc
     1621 gcagcgcggg gttacctggc cccgcccggg ccccattatg accgcaacgt tggacggaac
     1681 cgagcctgtt tcggctccgg tttcatcaag cgtgcgtggg gatgaacgag gcggggcatg
     1741 gtaaccccgc gctacgtttt atgggtaatt actccgagca agacagctgg ttaccggagg
     1801 ccgagggcct tccaggggtt gggcatctgg ccgaccggga tctcgatcag ggtcggctca
     1861 ttggccttga tcgactcttc gatcgcggtc tgcaacccgt ccgccgtctc ggttcgccgt
     1921 ccaacgacgc cgaacgcctc ggcgagcttc acgtagtccg gattctggag cgacgaggcg
     1981 atttcccggc caccgaggtc gacctgctgg attcgcttga cgttgccgta ggccgagtcg
     2041 ttgaagacga ggacgactgc cgggatggtg tgctgggcca tcgtcgccag ctcgttgagc
     2101 gcgaagccga agccgccatc gccgttgatc gagagcaccg gcgcgtccgg cacgccgacc
     2161 ttggcgccaa gggccgtcgg gaagccatag ccaagcgtgc cctggtagcc ggaagtgatg
     2221 taggtgttcg gctcgtagac cggcattgcg aagtttgacc aatagccgag ctgggtcatc
     2281 tcgctgacga cgacgccgtt ctccggaagc gcgttccgca tcgcccggcc gaaatcagcc
     2341 tggggcagga tgctcttgat gcgctcgttc acgcgcttct tgagatcctc gagctcggcc
     2401 ttgcgcgacg gccgaccgcc gacctgctgt tcgagcaggt cgatcagcgc cgcgagagca
     2461 ggcttggcgt cgccgaccat cgaaaccgtc ggcgtgatgt ctcgctcgat ctcctccggg
     2521 tcgatatcga tccgcagaat gaccgtctcc gggttcatcg gccgcggctg gctcaggtta
     2581 tcgaggaagc gggtgccgac gacgaggacg acgtccgcct tcggccgcag ctcgagctcg
     2641 ccgacctggt tctgggcgag gtagctgtga tcggagaccg cgcccttgcc gttggcgctc
     2701 atcacgaccg gcgcctggag cagctcggcc aattgccgca actcgtccca tgcccggccc
     2761 cggatcacgc cgccaccggc atagatcacc gggttccggg cgccggcgag gtactgcgcc
     2821 gctgcttcca gccggccagc cgccgggacc agcagtgtcc gctcggcaga cggcgccagc
     2881 agcgcgacat cttcctcggc gaacagcgtg tccggcggga cctcaatctc gaccggccgc
     2941 attcgcccgg aacgcaactc ggcaaaagcc ttgctgacca cgcccgggat ctcgccggga
     3001 gtcatcgccc gctcggcgct cttggtgatg tgacggatca tgcccagctg gtcgtggatc
     3061 tcgtggagca ggccgcgacc gtgatcgatc agatcggact tgatctgccc ggtcacgcag
     3121 aggaccggcg agttgcaggc gtaggcggtc gagagggcgg ccgtcgcgtt gagcaagccc
     3181 ggtcccggaa cgacaacgca gacaccctcg cgcccggtca cccgcgcgta gccatcggcc
     3241 atgtaggccg tcgcctgctc gtggcgagtg tggatgatgc ggaggcgatc ttgccgtgcc
     3301 cagaaggcat tgaatagccc gtcgagctgg acccccggca ggccgaagag ggtatcgacg
     3361 ccgttccgaa tcagcatctc agccagtgcg tcaccaccgg tcatgcgctg cgtcgttgcc
     3421 gcgacttcgg attgatcaac ggtggaactg actgccatga acttgcctca ctgaatcgaa
     3481 tacggtcgag tgttccaacg gggcattgta ctcgctgccc aacaccatca cgcgcgctcc
     3541 ggagccggcc ggttgtactt cgtcatcaga tcccggaccc ggtcgagcgg catcgcgtgc
     3601 gacgtccggc cgttgactcc ggtcatcgtc gtcgccatgc aaagcgcatt gatgatcgcc
     3661 tcttcggtcg catcggcggc agcttcgaac agcggcgtca tcgcgtccgg ttggaggctc
     3721 cggaccggga acggctcgaa tggctcgccg gcgatcatgc cccggttggc cgtcgagaag
     3781 gccaggaaga gatcgccgct gccgttattc ccgttgccgc cgacccgggc gagcccaatc
     3841 gtcgcccgct gcgcgatgcg ccggcactgg tcgggcagga gcggcgcgtc ggtcgccagg
     3901 atgatgatga tcgatccctc tttgggcgct ccctggtggc tcggcacctc gtcgaacccg
     3961 atctccgtcc caatcggcac cccgtcgatg cgcaaggtgt gccgcgcccc gtagttcgcc
     4021 tgcacgagga caccgaccgt ccagccgcca tgcttctcgg acacgcgtcg gctggaggtg
     4081 ccgatcccgc ccttgaactc gtggcaattc atgccggtgc cgccgccgac gcagccttcg
     4141 gccaccggac cggatctcgc cgcgtcagca gccgcgtaca gatgctccgg cttgacgtgg
     4201 aagccattga tgtcgctcag ccatccgtcg tacgtctcgc cgacgaccgg cagatgaaaa
     4261 tccggctccc gctcgtaggt gaatcgctgc cggtcatacg cgatcagcgc gtcccgcacg
     4321 acgccgacgc tccccgtgtt cgtgatcgct acggcggacg tcagaaggcc cgattcgttg
     4381 acccaggcga tgccggtcat ctcgccgttg ccgttgagct ggtggtagcc ggcgaagatc
     4441 gcctcctcgc cgacgttgcc gccgtgcggc tgc
//
LOCUS       DASSGX010000598         2264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_537531, whole genome shotgun sequence.
ACCESSION   DASSGX010000598 DASSGX010000000
VERSION     DASSGX010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537531"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="VFI12_10860"
     CDS             <1..888
                     /locus_tag="VFI12_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056955.1"
                     /translation="RGLASIHVPRRAMRLARTVGLAVIIALTLANIVWAVQDWHLHDM
                     NVYWTAGEMFRNTGNPYTLAPGVDDNSVYRYAPWFALVWMPFTELPRLAVNVLWSAVL
                     IVTSFTAVWPVLRAGGRTALPFAILMFGIFFGMAAGGNVHGLMIAWLAFGVERRSGPI
                     WIALAASLKAVPILYVLVYAGRGEWRKVVEAVALTAILVAPMLLFDRPAMTSDFGISN
                     SLAQIHPAIFWGSAAASVLVAVWVAFRLRLYGWLATGAAAYFCLPRVFLYDVTFILPG
                     IAESITAARRVHGHPDPRG"
     gene            863..1729
                     /locus_tag="VFI12_10865"
     CDS             863..1729
                     /locus_tag="VFI12_10865"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HET7056956.1"
                     /translation="MATLTRAASVGRSLPLVRAARLLVLAIVIGWSISNLAFHVAAWN
                     LSDMDAYWNAAMRLRGGQPLFPAVADPSAADVYRYSPWFAAAWVPLTFAPKVIVGVVW
                     SIVLLAATAVALWPLRRGGLTSLAAAFLLGSFLVWAASVGNVQPLLIAALVHGMERRS
                     GPLWIGLTASLKAVPFLYVLIYIGRREWVRAALAVAVTAVLVAPFLLVSLANYPAGSG
                     DAPSPLLSVSPILLVATVAGLAGVAVWLAGRRSRFDRLAAATAALTALPRITLLDLPQ
                     LLVGVRESSRKP"
     gene            1762..>2264
                     /locus_tag="VFI12_10870"
     CDS             1762..>2264
                     /locus_tag="VFI12_10870"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HET7056957.1"
                     /translation="MALAIVIGWSLANAIQRIGDWSLSDMDAYWNAATRLREGLPLYP
                     AVPDSGAADVFRYAPWFAWAWVPLTFLPKAAVAVAWSALLVGASAVAIMPIVRQPSLT
                     RIAAAALLGTILLWSAASGNVQPVIVAILVLGIEGRSGPLWVAIAASLKIFPIGFALV
                     YAGRREWR"
BASE COUNT          274 a          755 c          787 g          448 t
ORIGIN      
        1 cgaggtctcg ccagcattca cgttccgcga cgcgccatgc gcctggcgcg gacggtcggc
       61 ctcgccgtga tcatcgctct cacgctggcc aacatcgtgt gggcggtcca ggactggcac
      121 ctgcacgaca tgaacgtgta ctggaccgcc ggcgaaatgt tccggaatac cggcaatccg
      181 tacaccctgg cacctggcgt cgacgacaac tcggtatatc gatacgcccc gtggttcgcc
      241 ctggtgtgga tgccgttcac cgagcttccg cggctggccg tcaatgtctt gtggtcggcg
      301 gtgctgatcg tcacctcgtt cacggccgtg tggccggtgc tgcgcgccgg cggccgaaca
      361 gcgcttccgt tcgcgatcct catgttcggc atcttcttcg ggatggccgc gggcggaaac
      421 gtccatgggc tgatgatcgc ctggctcgcg ttcggggtcg aacgccggag cggaccgatc
      481 tggatcgcat tggccgcctc gttgaaggcg gtaccgatcc tctacgtgct cgtctacgcc
      541 gggcgtggcg aatggcgaaa ggtcgtcgaa gcggtcgcgc tgacggccat cctggtcgcg
      601 ccgatgctgc tgttcgatcg gccggcgatg acgtcggact tcgggatcag caactcactg
      661 gcgcagattc atccggcgat cttctgggga agcgcggccg catcggtcct ggtggccgtg
      721 tgggtagcct tccggttgcg gttgtatggg tggctggcga cgggagcggc agcctacttc
      781 tgcctcccac gcgtattcct gtatgacgtg accttcatcc tgccgggtat cgccgagagc
      841 atcaccgctg cgcggcgcgt ccatggccac cctgacccgc gcggctagcg tcggccggtc
      901 gctcccgctg gtacgggcgg cgcggttgct cgtgctggcc atcgtcatcg gctggagcat
      961 ctcgaatctc gccttccacg tcgcggcatg gaacctgtcg gacatggatg cctactggaa
     1021 tgccgccatg cgattgcgag ggggccaacc gctgtttccg gcggtggccg atccgagcgc
     1081 ggcggacgtc taccgctact caccctggtt cgccgcagca tgggttccgt tgaccttcgc
     1141 gcccaaggtc atcgtgggcg tcgtctggtc gatcgtgctg ctggcggcga cggccgtcgc
     1201 cctatggccg ctgcgtcgcg gcggcctcac ctcgcttgcg gcggcctttc tgctcggcag
     1261 cttcctggtc tgggccgcgt cggtcggcaa cgtgcagccg ctgctgatcg cggcgctcgt
     1321 gcacgggatg gaacggagga gcgggccgct gtggatcggg ctcacggcct cgctgaaggc
     1381 ggtgccgttc ctgtacgtcc tcatctacat cgggcggcgc gagtgggtgc gcgcggcgct
     1441 tgcggtcgcg gtcacggctg tgctggtcgc gccgttcctg ctggtaagcc tcgccaacta
     1501 cccggccggc tcgggtgacg ccccgtcacc gctcctgtcg gtctcaccga tcctgctcgt
     1561 cgcaacggtg gccggcctcg cgggggtggc cgtctggctc gctgggcgcc gttcacggtt
     1621 cgatcgcctg gcggcggcga cggccgcgct gacggcactg ccgcgaatca cgctgctgga
     1681 cctgccgcag ctgctggtcg gcgtccggga gtcttcgcgc aagccatgac ccgagaccgg
     1741 ctggtgcgca tggctcgcat cgtggccctg gcgatcgtga tcgggtggag cctcgccaat
     1801 gccatccagc gtatcggcga ctggtcgctc tccgacatgg acgcgtactg gaacgccgcc
     1861 acgcgattgc gcgagggcct gcccctctat cccgccgttc ccgactcggg agcagccgat
     1921 gtgttccggt atgcgccgtg gtttgcatgg gcgtgggtgc cgctgacctt cctgccaaag
     1981 gccgcggtgg cggtcgcgtg gtcggcgctg ttggtgggcg cctcggccgt cgcgatcatg
     2041 ccgatcgttc gccaaccgag cctgacacgc attgccgccg ccgccttgct gggcacgatc
     2101 ctcctctggt cggccgcgag cggcaacgtt cagccggtga tcgtcgccat cctggtgttg
     2161 ggtatagaag ggcgcagcgg accgctgtgg gtcgcaatcg cggcgtccct caagatcttt
     2221 ccgatcgggt tcgcactcgt gtacgccggc cgacgggagt ggcg
//
LOCUS       DASSGX010000599         4333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1192989, whole genome shotgun sequence.
ACCESSION   DASSGX010000599 DASSGX010000000
VERSION     DASSGX010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4333
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1192989"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..546)
                     /locus_tag="VFI12_10875"
     CDS             complement(40..546)
                     /locus_tag="VFI12_10875"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HET7056958.1"
                     /translation="MNIDDPRFSLVFVDCEATGPCPGKGELTEFGAVEFRTRQTFHGI
                     LAESKPQEDNPHLSELTGRRFDPVDVFAKFADWLATVCSGRPIFISDNPAFDWQWINY
                     GFYHALGRNPFGHSGRRISDFYASLVGDFWHSQAWKHLRITPHDHNPVHDAMGNVEAF
                     DRILNGER"
     gene            complement(546..1478)
                     /locus_tag="VFI12_10880"
     CDS             complement(546..1478)
                     /locus_tag="VFI12_10880"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET7056959.1"
                     /translation="MGNRLTRDQEGLLLGLIGVLAFSFSLPATKEAVPAFGPILLSLG
                     RAEGAAILAGLVLLIRREPFPGRQHLRSLLLIGAGVVVGFPILTSYALKHVPSAHGAV
                     VIALLPAATAAFGVILARERPPRAFWGAVLIGMVAVLIFAIIQGAGKPQWEDALLLGA
                     VVAAAFGYAEGGRLSREIGGWRVISWAVVIAAPFLLPFVIADLPNVDWHASGGEWLGL
                     AYVSFVSMYLGFLAWYRGLALGGIARVGQLQLAQPVLTLVWSWLLLGESIGWAEIIAG
                     LVIVGSVALTRRFRYDVGTNPDVAEPAPGMVAIE"
     gene            complement(1493..2287)
                     /locus_tag="VFI12_10885"
     CDS             complement(1493..2287)
                     /locus_tag="VFI12_10885"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HET7056960.1"
                     /translation="MAQVARFARIRSLFLVFSLLAGFLIWALPTDAARSDKEWDIPDD
                     AVPAEYIESIDGDTFDARVMQDNGQWRDERIRLIGIDTPETNYSYGNHPECYGKEAST
                     RSEGVLLDAKEIWLSKDVSDRDRNGRLLRYVWVVSKLDGKVHFLNEDLVKEGYALAKT
                     YKPDTKYQRELDAAERSAIEEARGMWLYCDASVSMDPDKENPDTGPDKTPIDRTKTPV
                     DDEDAVCSFFDTFDEAQDFMDLYPEIADVIDKDGDGIACEDYFFGN"
     gene            complement(2441..2851)
                     /gene="tnpA"
                     /locus_tag="VFI12_10890"
     CDS             complement(2441..2851)
                     /gene="tnpA"
                     /locus_tag="VFI12_10890"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HET7056961.1"
                     /translation="MPFWRLHYHLIWTSFGRDSVIDEEVERIIRASIASTARSLNLIP
                     HAVGMVPDHIHVVASIPPNVSISETVGRLKGAASHAVNDGNSRGTPFRWQREYGALSV
                     SDRGLPVVTEYALNQRQRHAANDLIATLERIESA"
     gene            complement(2976..3914)
                     /locus_tag="VFI12_10895"
     CDS             complement(2976..3914)
                     /locus_tag="VFI12_10895"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HET7056962.1"
                     /translation="MAAKRNKVIITCAVTGAVHVPSQSEYLPITPEQIAENAIGAAEA
                     GAAILHLHARNPENGRPTADPEMFMQFLPEIKERCNAVINITTGGGQGMTLEQRLAAA
                     MRAKPEMCSLNMGSMNFGRFGIAKRIKEFRFDWEAEDLEASRDYIFRNTYKDIAHILR
                     ELGEGHGVKFEFECYDVGHLYNLAYFVDEGLVKPPFFVQTIFGILGGIGTDVEDLLHM
                     KRTADRLFGDAYQWSILGAGRHQINLVTMGAIMGGNVRVGLEDNVYLGKGELAKSNAE
                     LVAKTARILNELSLEIATPDEAREILQLKGVHNVGF"
     gene            4070..>4333
                     /locus_tag="VFI12_10900"
     CDS             4070..>4333
                     /locus_tag="VFI12_10900"
                     /inference="COORDINATES: protein motif:HMM:NF017451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HET7056963.1"
                     /translation="MVWQQGIQLVNQVYRCTKSWPSHEMFGLTSQVRRAAVSVPANIA
                     EGQGRNGQREFVNFLGIANGSLCELETLLVIARDQGYLAADQEV"
BASE COUNT          795 a         1384 c         1358 g          796 t
ORIGIN      
        1 tgaaataagg cgacagttcc gtcgtttcga ggatagggtt caccgttcgc cgttgaggat
       61 gcggtcgaag gcttcgacgt tgcccatcgc gtcgtgtacc gggttgtggt cgtgcggcgt
      121 gatgcgcagg tgcttccagg cctggctgtg ccagaagtcg ccgaccaggc tggcgtagaa
      181 gtcgctgatc cgccggccag agtggccgaa cgggttgcgg ccgagcgcgt gatagaagcc
      241 gtagttgatc cactgccagt cgaaggccgg gttgtcgctg atgaaaatcg gccggccgct
      301 gcagacggtc gccagccaat ccgcgaattt ggcgaagacg tcgaccgggt cgaatcgccg
      361 ccccgtcagc tccgagaggt gcgggttgtc ctcctgcggc ttcgattccg ccaggatgcc
      421 gtgaaacgtc tgccgcgtcc ggaactcgac cgcgccgaac tcggtcagct cgcccttgcc
      481 aggacacggg ccggtcgcct cgcaatcaac gaagaccagg ctgaagcgcg gatcatcgat
      541 gttcatcact cgatcgccac catgccggga gcgggctcag cgacgtccgg gttcgtcccg
      601 acgtcgtagc ggaagcggcg ggtgagcgcg accgatccga cgatcacgag cccggcgatg
      661 atctccgccc agccgatcga ttcgccgagc aggagccacg accagaccaa agtcaggacc
      721 ggttgcgcga gctggagctg gccgacccgc gcgatcccgc cgagcgccag tccccggtac
      781 caggccagga agccgaggta catgctcacg aacgagacat aggccagccc gagccactcg
      841 ccaccgctgg catgccaatc cacgttcggg agatcggcga tcacgaacgg cagcaggaac
      901 ggcgcagcaa tcaccactgc ccatgagatc acccgccagc cgccgatctc ccggctcagg
      961 cgtccgcctt ccgcatatcc gaacgcggcc gcgacgaccg ccccgagcag gagcgcgtcc
     1021 tcccactgcg gcttgcccgc gccctggatg atcgcgaaga tcaggacggc caccatgccg
     1081 atgaggaccg cgccccagaa ggcgcgcggc ggccgttccc gggccaggat tacgccgaac
     1141 gccgccgtcg ccgccggcag cagagcgatc acgaccgccc cgtgcgcgga cggcacgtgc
     1201 ttgagggcgt acgaggtcag gatcgggaag ccgacgacca cccccgctcc gatcagcaac
     1261 agcgagcgga gatgctggcg ccccgggaac ggctcgcggc gaatcaagag gacgagcccg
     1321 gccaggattg cggcgccctc cgcccggccg aggctgagca agatcggccc gaaagccggg
     1381 acggcctcct tcgtcgcggg gagcgagaag ctgaaggcga gcacgccgat cagcccgagc
     1441 agcagccctt cctgatcgcg cgtcagccga tttcccacga tgcccccgtc actcagttcc
     1501 cgaagaagta atcctcgcag gcgatgccgt cgccgtcctt gtcgatgacg tcggcgatct
     1561 cgggatacag gtccatgaag tcctgcgcct cgtcgaacgt gtcgaagaag gagcagaccg
     1621 catcctcgtc atcgaccggt gtcttcgtgc ggtcgatcgg cgtcttgtcc gggccggtgt
     1681 cgggattctc cttgtccggg tccatcgaga ccgaggcgtc gcaatagagc cacatgcccc
     1741 gcgcctcctc gatggccgat cgctcggccg catcgagctc gcgctggtat ttcgtgtcgg
     1801 gcttataggt cttggcgagc gcgtaaccct ccttgaccag gtcctcattc aggaagtgga
     1861 ccttgccgtc gagcttggag acgacccaga catagcggag caggcggccg ttgcggtccc
     1921 gatcgctgac gtccttcgag agccagatct ccttggcgtc gagcagcacc ccctcgctgc
     1981 gggtactggc ttccttgcca taacactcgg ggtggtttcc ataggaatag ttggtttcgg
     2041 gcgtgtcgat cccgatcaac cggatccgct cgtcgcgcca ttggccgttg tcctgcatca
     2101 ctcgcgcgtc gaatgtgtcg ccgtcgatcg attcgatgta ctcggccggg accgcgtcat
     2161 ccgggatgtc ccattccttg tcggaccgcg cggcatcggt tggcagcgcc cagatcagga
     2221 acccggccag cagcgagaag acgaggaaca gcgatcgaat ccgggcgaag cgagcgactt
     2281 gggccaagcg aagcacctcc agggaacaac caacctgccc ctaatcctat cccgtcacga
     2341 tgcggtcggg ctatacccct ccaaccatcg tgccggcgcc gcccgggcgt aaacacccgg
     2401 gctgaatcca ataagccagc taaagcaggc tgggtaagcg tcacgctgat tcgatgcgct
     2461 ccaatgtcgc aatgaggtcg ttggcagcat ggcgttggcg ctgattgagc gcgtattcgg
     2521 tgaccacggg cagtcctcga tcgctaacgc ttagcgcgcc atattcccgt tgccatcgaa
     2581 acggagttcc gcgtgagttg ccgtcattga cagcatgtga tgccgccccc ttcaggcgac
     2641 caaccgtctc cgagatcgag acattaggag gaatcgacgc cacaacatgg atatggtcag
     2701 gaaccatccc gactgcatgc gggatcagat tcagtgagcg ggccgtgctt gcgatcgatg
     2761 cgcgaatgat tcgctcgacc tcctcgtcaa tcactgaatc gcggccaaac gatgtccaga
     2821 tcaggtgata atgcagcctc caaaacggca agccgaaacc ctccccgcac cctttcaact
     2881 cggccacccc agcctgcttt agctggctta ttggattcag ccctgtgctt tagcgctggg
     2941 cgactccggt gacccgtttc aagatccatt ggggctcaga accccacgtt gtggacgccc
     3001 ttgagttgca ggatttcccg ggcctcgtcc ggggtggcga tctcgagcga cagctcgttc
     3061 aggatccgcg ccgtcttcgc gaccagctca gcgttgctct tcgccagctc ccccttgccg
     3121 aggtagacgt tgtcctcgag cccgacccgg acgttgccgc ccatgatcgc gcccatggtc
     3181 acgaggttga tctggtgccg gcccgcgccg aggatcgacc actggtaggc atcgccgaag
     3241 aggcggtcgg ccgtccgctt catgtggagc agatcctcga catccgtgcc gatgccgccg
     3301 aggatgccga agatggtctg gacgaagaag ggcggcttga ccagtccctc atcgacgaag
     3361 taggcgaggt tgtagaggtg gccgacgtca tagcactcga actcgaactt gacgccgtgg
     3421 ccctcgccga gctcgcgcag gatgtgggcg atgtccttgt aggtgttgcg gaagatgtaa
     3481 tcgcgcgagg cttcgaggtc ctccgcctcc cagtcgaagc ggaactcctt gatccgcttg
     3541 gcaatcccga agcggccgaa gttcattgag cccatgttca gggagcacat ctccggcttg
     3601 gcccgcatgg ccgccgccag tcgctgctcg agcgtcatcc cctggccacc gccggtcgtg
     3661 atgttgatca ccgcgttgca gcgctctttg atctcgggga ggaactgcat gaacatctcc
     3721 ggatcggcgg tcggtcgccc attctccggg ttccgggcgt gcaggtgcag gattgccgcg
     3781 ccggcctcgg ccgcgccgat tgcgttctcg gcgatctgct ccggcgtgat cggcaggtat
     3841 tcggattgcg acgggacatg caccgcgccc gtaactgcgc aggtgatgat gaccttgttc
     3901 cgcttggcag ccaatggact cgctcctgtt gatactcggc agaatggcaa ttgtcgtcac
     3961 tctaccgtag gaattcgacg gtagtatttg tcacaaggtt ttccgttttc cgttttccga
     4021 atttgtcgct gaggggcacg cgttggggag ccagagttat cgggatctga tggtgtggca
     4081 gcagggaatt cagctcgtga accaggttta ccggtgcaca aaatcgtggc caagccacga
     4141 gatgtttggc ctgacgtcac aagtcagacg tgcggccgtt tccgttcccg ccaatattgc
     4201 ggagggacag ggaagaaacg ggcagcgcga attcgtcaat tttctcggga tcgccaacgg
     4261 gtcactctgc gagttggaaa ctctgctcgt gatcgcaaga gaccaaggct atctggcagc
     4321 ggaccaggaa gtt
//
LOCUS       DASSGX010000600         2115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_756296, whole genome shotgun sequence.
ACCESSION   DASSGX010000600 DASSGX010000000
VERSION     DASSGX010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756296"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..1037)
                     /gene="tilS"
                     /locus_tag="VFI12_10905"
     CDS             complement(54..1037)
                     /gene="tilS"
                     /locus_tag="VFI12_10905"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HET7056964.1"
                     /translation="MQRGSGSGVIQRFLAFLDDQGLTGTSAGIAFSGGRDSSALAICA
                     TEARNADRLNPILVHVDHGVRQDSSEDRDIVRQSAAVLDAELRSIDIKSLSTQSTEAE
                     MREARYLALHSILAPLGITTVLTGHHARDQAETVLLHLARGQGLEGATGISPVESLRF
                     GAIDITVIRPFLHEDPVALADLVAGYGLPIVEDETNALVDRARNLVRHQVLPVLCEIN
                     PGAVMNIARSTEILREENQTLVVSTSDTLRVVAGDGGLNGAGLSDVPVAIQRRVIRAW
                     VRQQTGIELTFDRTEALRLAANAGTGNVVLEIGDGWTARQIRRTITLQREK"
     gene            complement(1066..1137)
                     /locus_tag="VFI12_10910"
     tRNA            complement(1066..1137)
                     /locus_tag="VFI12_10910"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1103..1105),aa:Met,seq:cat)
     gene            1270..>2115
                     /locus_tag="VFI12_10915"
     CDS             1270..>2115
                     /locus_tag="VFI12_10915"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HET7056965.1"
                     /translation="MTSHLLDGLNAEQQAAVLATNGPVLVVAGPGSGKTRVLTHRIAY
                     LIEEMGVQAEEILAVTFTNKAAREMRERIDHLIGSPRSTSGLWMGTFHSIGVRILRET
                     PGFVADRLGILPNFLIYDDADQIGVAKQAITAIGQDPKKVAPRRMLSRISAAKSQLFT
                     PAEYRESEVQTYDDELVARVFEEYERLLRRNNALDFDDLLTMPIRLFDAAPMVLERYQ
                     SRFSYILVDEYQDTNRVQYVLVSALAGKYRNLFVVGDPDQSIYAWRQADIRNILDFER
                     DYPDAK"
BASE COUNT          386 a          674 c          661 g          394 t
ORIGIN      
        1 ccgctcgact ccatcttcct tgtgcgacaa gaccttgaga ctccctgcaa ccatcacttc
       61 tctcgctgta gcgtaatcgt tcgtcgtatc tgccgtgcgg tccacccgtc gccgatctcg
      121 aggacgacgt tcccggtccc ggcattcgcc gccagccgca gcgcctcagt gcggtcgaat
      181 gtcagctcga tgcccgtttg ttggcggacc caggctcgaa tcacgcggcg ctgaatggcc
      241 accggcacat cgctcaaccc ggcgccgttc agcccgccat cgccggctac gactcgcagc
      301 gtgtcgctgg tgctaacgac gagggtctga ttctcttcac gaagtatctc ggtggaccgg
      361 gcaatgttca taaccgcgcc cgggtttatc tcgcagagca ccggcaggac ctggtgccgc
      421 accagattgc gggcgcgatc gacgagcgcg ttggtctcgt cctcgacgat cggcaatcca
      481 taccctgcga cgaggtcggc aagcgcgacc ggatcttcgt gcaggaatgg ccggataacc
      541 gtgatatcga tcgcgccgaa acggagcgac tcgaccgggc tgatcccggt cgcgccttcc
      601 agaccctgcc cccgtgcaag gtgcagcagc acggtctcgg cctggtcccg cgcatgatgg
      661 ccggtcagca ccgtcgtgat cccgaggggc gcgaggatgc tatgcagcgc gagataccgg
      721 gcttcccgca tctcggcttc ggtcgactgc gtgctgaggg atttgatgtc gatcgatcgg
      781 agttcggcgt cgaggacggc cgccgattgc cggacgatgt cgcgatcctc actgctgtcc
      841 tggcggacac cgtgatcgac gtggacgagg atcgggttga ggcggtcggc attgcgggcc
      901 tcggtggcgc aaatagcgag cgcgctcgag tcgcggccgc ccgagaaggc gatcccggcg
      961 gacgtgccag tcaggccctg gtcatcgaga aaagcaagga accgctggat gacccctgaa
     1021 ccggatccgc gctgcatgtc aatcctgatg ttggcgaggg aagagtgccg acgggtggat
     1081 tcgaaccacc ggccaaaggc ttatgagtcc tttgctctgc cactgagcta cgtcggccca
     1141 aaacgcaccc aaaagggtac caattcgtgc ctgggccgtc aatgaacgtg tgccggttcg
     1201 ccatgcctca ggccggtggt agaatcgatt gtcgaaacag acgttcgagc cgcaaagagc
     1261 atttactcca tgacatctca cctcctggac ggcctcaatg ccgagcagca agcagccgtc
     1321 ctcgcgacaa atgggcctgt attggtcgtg gccggtccgg gctccgggaa gacgcgcgtc
     1381 ctgacgcacc ggatcgccta cctgatcgag gaaatgggcg ttcaggcgga ggagattctc
     1441 gccgtcacct ttaccaataa ggcggctcgc gagatgcggg agcggatcga tcatctgatc
     1501 ggctccccgc ggtcgacatc cggtctttgg atgggcacat tccactcgat cggcgtccgg
     1561 atcctccgcg agaccccggg gttcgtcgcg gaccggctcg gcatcctgcc caacttcctg
     1621 atctacgacg atgccgacca gatcggcgtg gcaaagcagg cgatcaccgc catcgggcag
     1681 gacccgaaga aggtcgcgcc ccggcggatg ctctcgcgca tctccgcggc caagagccag
     1741 ctcttcacgc cggccgagta tcgggagagc gaagtccaga cctacgacga tgagctggtt
     1801 gcgcgggtct tcgaggagta cgagcggttg ctgcggcgca ataacgcgct cgacttcgat
     1861 gacttgctga cgatgccgat tcgcctcttc gacgccgcgc cgatggtgct tgagcgatat
     1921 cagtcgcgct tcagctacat ccttgtcgac gagtaccagg acacgaaccg tgtccagtat
     1981 gtgctcgtat cggcgttggc cggcaagtac cgcaacctgt tcgtcgtggg cgaccccgac
     2041 cagtcgatct acgcgtggcg acaggccgac attcgcaaca ttctcgattt cgaacgggac
     2101 tatccggatg cgaag
//
LOCUS       DASSGX010000601         6316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1629586, whole genome shotgun sequence.
ACCESSION   DASSGX010000601 DASSGX010000000
VERSION     DASSGX010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6316
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1629586"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            259..576
                     /locus_tag="VFI12_10920"
     CDS             259..576
                     /locus_tag="VFI12_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056966.1"
                     /translation="MAAVGLWGLFTFMRGGVMEGSISGALVIGQILIGVQGLMGLILF
                     ADGFRPADSVHILYGVAAFVTMPFVWSYARERHPRQGLFFYSLAALFIAGLAIRGITT
                     GGG"
     gene            719..1267
                     /locus_tag="VFI12_10925"
     CDS             719..1267
                     /locus_tag="VFI12_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HET7056967.1"
                     /translation="MDTQARDYQEVIDQALGLVYSHHNRLITRLAPSSFVPLDIEDLR
                     EGPLGRDLQRLANLARGQTREEKELVLRAIEDVLQLLFWPAAADEYTVPRSFWDTDLG
                     RMMALAKFRAYEPHELVSIGVAAETLQVTRPTIYRWMDDRRLNYVKDEISGRTFIVRR
                     DLENLTTVGSDLDLEPSQGGVY"
     gene            complement(1323..2522)
                     /gene="alr"
                     /locus_tag="VFI12_10930"
     CDS             complement(1323..2522)
                     /gene="alr"
                     /locus_tag="VFI12_10930"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET7056968.1"
                     /translation="MIELQETTLDVVAALPGRATRAIIDLDALAGNVAALRRLLAPST
                     QLMTVVKGDGYGHGAVMVSRCALASGASMLGVATVGEAAELRQHQVTAPILLLGPCDP
                     TEVERALALDVELMVAGSELLAAAAAAERLGKIARVHLKIDTGMHRYGVTPRDALAVI
                     DRIETIDSVDLVAICTHYAAADETEHPANIRQAAIFEGVAAQIAVRLGRPIAIHSANS
                     AATLRGMATGTDIARCGLAMYGLAPSSEVPLPGDLRPVMSLISRLARVHCGETGSGVS
                     YGHTYIAEADERFGLIPIGYADGYRRALSNRSWVAVAGQRCPIRGNVCMDQMIAGNVP
                     ESADEGSLVGIAGPSGGGPGFDELAELAGTIPYEMVVGINRRVPRYYVADGKVVATLI
                     EGKLETV"
     gene            complement(2519..3502)
                     /locus_tag="VFI12_10935"
     CDS             complement(2519..3502)
                     /locus_tag="VFI12_10935"
                     /inference="COORDINATES: protein motif:HMM:NF013425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP/ATP-dependent (S)-NAD(P)H-hydrate
                     dehydratase"
                     /protein_id="HET7056969.1"
                     /translation="MTDSGVTIREFGTEDARRVLPRRPAGAHKWGVGGVLIVAGSPGY
                     VGAAALCAMAAGRAGAGIVNCAVHRSLVTAIVGVVPEAGFTLLPEGDMGTTGKRVLDA
                     IDKKAEKSAAFVVRPGLGDDDYARDLMIALLGLAEQSPILSLGFGVPRGQVSEAAERH
                     SLLAYDKPILVDADGLNALARIDDWWTRLPEGRMVLTPHVGEFSRLMDMETREILADP
                     EGAAVSAALKFRQVVLLKGAPTIVTDGTTIYRATDAPPSLATGGSGDVLSGTIGALLA
                     QGLSLVDAANLGVYAGVKAARRLETELGTLGLVASDLPRAIAVELAALERS"
     gene            complement(3499..3942)
                     /gene="acpS"
                     /locus_tag="VFI12_10940"
     CDS             complement(3499..3942)
                     /gene="acpS"
                     /locus_tag="VFI12_10940"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HET7056970.1"
                     /translation="MYVPEFDPEKCGRIVVGIDLIEIPRIQRVLDDFGERFLNRVYTQ
                     RERDRYRDRISELAARFAAKEAISKALGTGIRGIKWREMEVVSNRRGKPIVVLHGTAA
                     ARADLLGITDFDISLTHSRTDAMAFVVGFRADVGPPPASQGTGEQ"
     gene            complement(3946..5103)
                     /gene="tgt"
                     /locus_tag="VFI12_10945"
     CDS             complement(3946..5103)
                     /gene="tgt"
                     /locus_tag="VFI12_10945"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HET7056971.1"
                     /translation="MTAFRYELIGTDQATRARRGRVHTRRGEIETPVFMPVGTQATVK
                     TLEPHEVEGAGAQIILANTYHLMLRPGVEIIQAAGGVHEFSRWQGPILTDSGGFQIFS
                     LSSQVKIREEGASFQSHIDGSHWHLTPERAIELQAGYGSDIMMALDHVVGYPAKERVV
                     ADAMHRTHRWLDRCIARAKIGDLEPGQGVLFGIVQGGMYPELRTESAQQIAERDVAGC
                     AIGGLSVGEPKDVMAEYLDISIEHLPEHKPRYLMGVGSPEDLWNGVAQGVDMFDCVLP
                     TRLARNAALFTPDGRINLRNRKYRDVFAPIDETCDCHTCRTYTAAYLHHLYRAGEILG
                     LKLGSIHNIRFLVRQMEAMRAAIMAGTFAEERHAFLDRYRVVGQPVAQEAA"
     gene            complement(5111..5728)
                     /locus_tag="VFI12_10950"
     CDS             complement(5111..5728)
                     /locus_tag="VFI12_10950"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HET7056972.1"
                     /translation="MNFLGIGYGEVLIIIVAALIIFGPDKLPEVAGQAARWIRDARKM
                     MTEMSGEFERNAGVREFKDAIEGEIRGVRKELEDAGVSVKKDLNAAAGTVTSTVKSAT
                     STKPTGSAKSTATAGANKAASTTPAAKTTARARASKADPYAGLVQLTEVAPKQRKAAI
                     VAAAEPATNGHANGSSEPIRETSEAVMRARERRLAAGYNRRIPAS"
     gene            5847..>6316
                     /gene="pth"
                     /locus_tag="VFI12_10955"
     CDS             5847..>6316
                     /gene="pth"
                     /locus_tag="VFI12_10955"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HET7056973.1"
                     /translation="MKIIVGLGNPGREYERTRHNIGFQVTDAFVGELSGVSQRSRFRA
                     VVREGVSGTTKYVVAQPQMYMNLSGVPVREIVNWYKIDLADLLVIYDDMDLPFGQLRM
                     RADGSAGGHNGMKSIISELGTFDFARLRVGIGRGGGSSTAHVLSRFRPEESATI"
BASE COUNT         1052 a         2143 c         2001 g         1120 t
ORIGIN      
        1 tcggcgacga acaacacgcc ggctgtcgcg aagacggcca ttcttgatcg cttccgccgc
       61 accggcgcga gcccggtggt cacggtgctg ccgcgcggcg tacccgggcc gatttggaac
      121 ggcgcaaacg tccgggcgcg gcaactacag ctcctcgcag atatctgatc ttgtgacgac
      181 catacgcccc cggagatacg tgccctggaa gcgatcctga caatccacga ccgcctttcg
      241 gtcaccgtgt tgctcttcat ggccgccgtg ggtctgtggg gcctgttcac attcatgcgc
      301 ggcggcgtga tggaggggag catcagcggc gcgctcgtga tcggccagat cctgatcggc
      361 gtgcaggggc tgatggggct gatcctcttc gccgatggct ttcggccggc tgattcggtc
      421 cacattctct atggcgtcgc cgccttcgtg acgatgccct tcgtctggtc ctatgcccgc
      481 gagcgacatc cccgccaggg ccttttcttc tacagcctgg ctgccctctt catcgctggg
      541 ctggccatcc gcggcatcac caccggcggc ggctgacttc acctgacccg acggtactga
      601 tcgggaatac gcaagttcgc acaattattg acacctcttg cacctgcgcg tacaatctga
      661 ccgtacaagg actacgtacg ccccccgcac gttgaatttg gctgcaaaag gagccgtcgt
      721 ggatacgcaa gcgcgggact accaggaagt catcgaccaa gccctgggac tggtctatag
      781 ccatcacaat cggctgatca cgcggcttgc gccgtcgtcg ttcgtgccac tcgacatcga
      841 ggatttgcgg gaggggccgc tcggccgcga cctccagcgc ctcgcgaacc ttgctcgcgg
      901 acagacgcgc gaggagaagg aactcgtgct ccgcgccatt gaggacgtct tgcaactcct
      961 cttctggccg gccgcggccg acgaatacac cgtaccgcgc tccttctggg acacggatct
     1021 cggccgaatg atggcgctcg cgaagttccg cgcctacgag ccgcatgagc tggtcagcat
     1081 cggcgttgca gccgagacgc tccaggtcac ccgtcccacg atctatcgct ggatggatga
     1141 tcgacgcttg aactatgtca aggacgagat cagcggccgc accttcatcg tccggcgcga
     1201 cctcgagaac ctgaccacgg tcggttcaga cctcgatctc gagccaagcc agggcggcgt
     1261 ttattagttc gaaccaaatg caaagcggcc ggggattcat tcccggccgc tttgcattgc
     1321 attcagactg tttcgagctt gccttcgatc agcgtcgcga cgaccttgcc gtccgccacg
     1381 tagtatcgcg gtacccgccg gttgattccg acgaccatct cgtatgggat cgtcccggcg
     1441 agctcggcca attcgtcgaa gccagggccg ccaccgctcg gtcccgcgat cccgaccaga
     1501 ctgccttcgt ccgcactctc cggaacattg cccgcgatca tctggtccat gcagacgttg
     1561 ccgcggatcg ggcaacgctg cccggcgacg gccacccacg accggttcga gagcgcgcgg
     1621 cgatagccgt ccgcataccc gatcgggatc agcccaaacc gctcgtcggc ctccgcaatg
     1681 taggtgtgcc cgtaactgac cccgctgcca gtctctccgc aatgcacgcg ggcaagtcga
     1741 gaaatcaggc tcatgaccgg gcgcaggtca ccaggcagcg gcacctccga actcggggcg
     1801 aggccgtaca tcgcaagtcc gcatcgcgcg atatcggtgc cggtcgccat cccgcgcagc
     1861 gtggcggcgc tgttcgccga gtggatcgcg atcgggcgcc ccaggcgaac agcgatctgc
     1921 gccgcgacgc cttcgaaaat cgcggcctgc cgaatgttcg ccggatgctc ggtctcgtcc
     1981 gcggcagcgt aatgggtgca gatcgcgacg aggtctacgg aatcgatggt ctcgattcga
     2041 tctatcacag ccagggcatc gcgcggcgtg acgccgtatc ggtgcatgcc ggtatcgatc
     2101 ttgagatgaa cgcgcgcgat cttgccgagc cgttcagctg cggccgcagc ggcgagcagc
     2161 tccgaaccgg ccaccatcag ctcaacatcg agcgccaatg cccgctcgac ctcggtcggg
     2221 tcgcagggac cgagcaatag gatcggggca gtgacctggt gctgccgtaa ctcggcggcc
     2281 tcgccgaccg tcgctacgcc cagcatcgac gcgccggatg ccagcgcgca ccggctgacc
     2341 atgaccgcgc catgcccata tccgtcaccc ttgacgacgg tcataagctg cgtcgaggga
     2401 gcaagcagcc ggcggagcgc cgcgacattc ccggccagcg catcaaggtc gatgatggct
     2461 cgcgtggcgc gtccgggtaa cgcggccacc acatcaagcg tcgtttcctg gagctcgatc
     2521 atgaccgctc cagcgccgcc agctcgactg cgatcgcgcg cggcaggtcg cttgcgacca
     2581 ggccgagggt cccgagctct gtttcgagcc ggcgggcggc cttgacgccg gcgtagacgc
     2641 ccaggttggc ggcatccaca agcgaaaggc cctgggcaag caacgccccg atcgtccccg
     2701 acagcacatc gccgctgccg ccggtcgcca gcgatggcgg ggcgtccgtt gcgcggtaga
     2761 tggtcgtacc atcggtcacg atcgtcggcg ctcccttgag caggacgacc tgccggaact
     2821 tcagcgccgc gctcaccgcc gccccctccg gatcggccag aatctcgcgg gtctccatgt
     2881 ccatcaggcg gctgaactcg ccgacatgcg gcgtcagcac catccggccc tcgggcaggc
     2941 gcgtccacca gtcgtcgatc ctggcgaggg cattgaggcc gtcggcgtcg acgaggatcg
     3001 gcttgtcgta cgcgaggagc gaatgccgtt cggccgcttc ggatacctgt ccgcgaggca
     3061 cgccgaaccc gagcgacaag attggtgatt gctcggccag tccaagcagt gcaatcatca
     3121 gatcgcgggc gtagtcatca tcgccgagac caggcctaac cacgaacgcc gcgctcttct
     3181 cggccttctt gtcgatcgcg tcgagcacgc gcttgccagt cgtgcccatg tcgccttccg
     3241 gcagcaatgt gaatccggcc tcgggcacaa cgccgacgat cgccgtgacc agcgatcggt
     3301 gtaccgcgca gttgactatg ccggcgccgg cccggcccgc cgccatcgcg cagagtgccg
     3361 ccgcgccgac atacccggga gatccggcca cgatcagcac accaccgacg ccccacttgt
     3421 gcgccccggc cggccgccgc ggcagaactc gccgagcgtc ctcggtcccg aattcacgta
     3481 tcgtcacacc cgaatctgtc attgttcccc tgttccctgg ctcgccggcg gcggaccaac
     3541 atcggcccgg aagccgacca cgaaggccat cgcgtcggtc cgcgagtgcg tcaacgagat
     3601 gtcgaaatcg gtgatgccca gcaaatcggc gcgcgcggcc gccgtgccgt gcagcacgac
     3661 gatcggctta ccacgccggt tcgagacgac ttccatctct cgccacttta ttccccggat
     3721 acccgtgccc agcgccttgg agatcgcttc cttggccgcg aatcgcgccg ccagctccga
     3781 tatccggtcg cggtatcgat cgcgctcgcg ctgggtgtac acgcggttga ggaaacgctc
     3841 gccgaagtca tccagaacgc gctgaatccg gggaatctcg atcaggtcga tgccgaccac
     3901 gatccggccg cacttttcag gatcaaattc cggcacgtac acggctcagg ccgcctcctg
     3961 cgctaccggc tgaccgacga cgcggtagcg atccaggaac gcgtgccgct cctcggcgaa
     4021 cgtgccggcc atgatcgcgg cgcgcattgc ttccatctgc cggacgagga agcggatatt
     4081 gtggatcgac ccgagcttca acccgagtat ttcgcccgcc cggtacaggt gatgcaggta
     4141 ggcggcggtg taggtgcggc acgtgtggca atcgcaggtc tcgtcgatcg gcgcgaacac
     4201 atcccggtat ttgcggttgc ggaggttaat ccggccgtcc ggcgtgaaga gtgcggcgtt
     4261 acgggcaagg cgagtcggca gcacgcagtc gaacatgtcg acgccctggg ccacgccgtt
     4321 ccagaggtcc tccggcgagc cgacgcccat caggtagcgc ggcttgtgct ccggcagatg
     4381 ctcgatcgag atgtcgaggt actccgccat cacatccttc ggctcgccga cgctcaggcc
     4441 gccgatcgcg cagccggcca cgtcccgctc ggcaatctgc tgcgcgctct cggtgcggag
     4501 ttccgggtac atgccgccct ggacgatgcc gaagagcacg ccctgccccg gctcgaggtc
     4561 gccgatcttc gcgcgcgcaa tgcaccggtc gagccatcgg tgcgtgcggt gcatggcgtc
     4621 cgcgaccacc cgctccttcg ccggatagcc aacgacatgg tcgagcgcca tcatgatgtc
     4681 cgagccgtag ccggcctgaa gctcgatcgc gcgctctggc gtcagatgcc agtgcgaacc
     4741 gtcgatatgt gactggaacg acgcgccctc ctcgcggatc ttcacctgcg aggagaggct
     4801 gaagatctgg aaaccgccgc tgtccgtcag gatcggtccc tgccagcgcg agaattcatg
     4861 cacgccgccg gccgcctgga taatttcgac gccggggcgc agcatcaggt ggtaggtgtt
     4921 ggcgaggatg atctgggcgc cggcaccttc gacctcgtgc ggctcgagcg tcttcacggt
     4981 cgcctgggta ccgacgggca tgaagaccgg cgtctcgatc tcgccccgtc gcgtatggac
     5041 ccggccacgg cgcgcgcgcg tcgcctgatc cgtgccgatt aattcgtacc ggaatgcggt
     5101 catgatcccg ctatgacgcc gggatccggc ggttatagcc ggccgccagg cgtcgctcgc
     5161 gggcgcgcat cacggcctcg ctcgtctccc ggatgggctc gctggagccg ttagcgtggc
     5221 cgttggtggc cggctccgcg gcggcgacga tggccgcctt gcgctgcttt ggcgcgacct
     5281 cggtgagctg gaccaggccg gcatagggat cagccttgga cgccctggcc ctggcggtcg
     5341 tcttggcagc aggagttgtg ctggcggcct tgttggcgcc cgcggtggcg gtggacttgg
     5401 ccgagccggt tggcttcgtg ctggttgcgg acttgaccgt gctggtcacc gttccggccg
     5461 cggcgttcag atccttcttg accgacactc ccgcgtcttc gagctccttg cggacgcccc
     5521 ggatttcgcc ttcgatggcg tccttgaact cgcgcacgcc ggcgttgcgc tcgaactcac
     5581 cggacatctc ggtcatcatc ttccgggcgt cgcgaatcca gcgcgctgcc tggccggcga
     5641 cctcggggag cttgtcgggt ccgaaaatga tcagcgcggc gacgatgatg atgaggacct
     5701 cgccgtaccc gatcccaaga aaattcatcg aggaagtccc tgcgtgctcg gcaattacac
     5761 agtcacattc tctgccgagg aactggtttg gagtatagca ttgaggatgc ggcggtccgg
     5821 aatcgccgat ggaagaaaga ggacgcgtga agatcatcgt cggattgggt aatccgggcc
     5881 gggaatacga gcgaacccgg cacaatatcg gctttcaggt aaccgatgcc ttcgtgggcg
     5941 agctcagcgg cgtctcccag agatcgcgct tccgggcggt cgtgcgcgag ggcgtttccg
     6001 gcacgaccaa atacgtcgtc gcgcagccgc agatgtacat gaacctgagc ggcgtcccgg
     6061 tccgcgagat cgtcaactgg tacaagatcg acctcgcgga cttgctcgtg atctacgatg
     6121 acatggacct cccgttcggc caattgcgga tgcgggcgga tggctcggcc ggcggccata
     6181 acggcatgaa gtcgatcatc agcgagctgg ggacctttga tttcgcccgt ctccgcgtcg
     6241 gcatcggccg cggcggcggg agcagcacgg cccacgtcct ctcccgcttc cggccggagg
     6301 aatcggcgac aatcga
//
LOCUS       DASSGX010000602         2533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1193133, whole genome shotgun sequence.
ACCESSION   DASSGX010000602 DASSGX010000000
VERSION     DASSGX010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2533
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193133"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2533)
                     /locus_tag="VFI12_10960"
     CDS             complement(<1..>2533)
                     /locus_tag="VFI12_10960"
                     /inference="COORDINATES: protein motif:HMM:TIGR01580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HET7056974.1"
                     /translation="MRPADIGGKPAKTNNPDWKCLAYDELSGKLVIPVGSAGHRWGDP
                     GKWNLEEVEEGGKGVRLQLSLAADNDSIQPVAFPYFGADAPHDFVATEHDDILVRNVP
                     VKELKLADGSKAFVATVFDLFCANYGVDRGFGGDNVAKSYDDDVPFTPAWAEKVTGVP
                     RQAIIQVAREFASNAEVTSGRSMVILGAGLNHWYHMDMSYRGIISLLIMCGCIGQSGG
                     GWSHYVGQEKLRPQTGWLPLAFASDWSRPSRQMNGTSFFYAHTDQWRYESLKMSEILS
                     PLAPEGDWSGSMIDYNVKAERMGWLPSAPQFDANPIEWGNRLKESGADPAQAIAEAFK
                     SGELTPGGLDPDNPVNWPRNMFFWRSNVLGASGKGHEYFLKHLVGSLHGVIGTDEEAA
                     GIERPKDVVWREDAPVGKLDLMVNIDFRMSTTSMYSDVVLPTATWYEKHDLNTSDMHP
                     FIHPLSAAVDPAWETKSDWNIFRAIASKFSDIAPEVLGVEHDLVMTPILHDTAAELAQ
                     PYEPKDWFKGECEPIPGKTMPNVTLVERNYPETYARFTSMGPLLEKLGNGAKGLNWDT
                     KKEVELLGDLNGRVLDGPTEGRPKIDTDIDACETILMLAPETNGEVAVKAWAALGKAT
                     GRDHDHLAEGKEEEKIRFRDVAAQPRKIISSPIWSGLESEHVCYNAGYTNVHELIPWR
                     TLTGRQQLYLDHHWMRAFGEGFVTWRPPIDTKTVRQVLGKLPNGNKEILLNILTPHQK
                     WGIHSTYTENLIMLSLNRGGPTVWISEDDAKAAGIVDNDWIEVFNVNGALTARAIVSQ
                     RIMPGSAIMYHAQEKLTNTVGSEITGQRGGIHNSVTRINMKPTHMI"
BASE COUNT          411 a          815 c          784 g          523 t
ORIGIN      
        1 gatcatgtgc gtcggcttca tgttgatccg cgtgacgctg ttgtgaatgc cgccacgctg
       61 gccggtgatc tccgaaccga cggtgttcgt gagcttttcc tgggcgtgat acatgatcgc
      121 cgatccgggc atgatccgct gcgatacgat cgcccgcgcg gtcagcgcgc cgttcacatt
      181 gaacacctcg atccagtcgt tgtcgacgat gcccgcggcc ttggcgtcat cctcgctgat
      241 ccacaccgtc gggccgccgc ggttgaggct gagcatgatg aggttctcgg tgtaggtcga
      301 atggatgccc cacttctggt gaggggtcag gatgttaagc aagatctcct tgttgccgtt
      361 gggcagcttg ccgagaacct gccggaccgt cttcgtgtcg attggcggcc gccaggtgac
      421 gaagccctcg ccgaacgccc gcatccagtg atgatcgaga tagagctgct gccgtccggt
      481 cagcgtcctc cacgggatca gctcgtgcac gttggtgtag ccggcgttgt agcagacatg
      541 ctcgctctcc agccctgacc aaatcggcga agagatgatc ttgcgaggct gcgcggccac
      601 gtcgcggaag cggatcttct cctcttcctt gccttccgcg agatgatcgt ggtcgcgccc
      661 ggtcgccttg cccaatgccg cccaggcctt gaccgcgacc tctccgttcg tctccggcgc
      721 gagcatcagg atcgtctcgc aagcatcgat gtcggtatcg atcttcgggc gtccctcggt
      781 aggcccgtcg agaacccggc cattcagatc accgagcagc tcgacctcct tcttcgtgtc
      841 ccagttgagg cccttcgcgc cgttgccgag cttctccaga agcggcccca tcgaggtgaa
      901 gcgcgcatag gtttccggat agttgcgctc aacaagcgtg acattcggca tggtcttgcc
      961 gggaatcggc tcgcactctc ccttgaacca gtctttcggc tcgtacggct gggcaagctc
     1021 agcagccgtg tcgtggagga tcggagtcat cacgaggtcg tgctcgacgc cgagcacctc
     1081 cggcgcgatg tcggagaatt tgctcgcgat cgcccggaag atgttccagt cgctcttcgt
     1141 ctcccacgcc gggtccacgg ccgccgagag ggggtggatg aacgggtgca tgtccgacgt
     1201 attgaggtcg tgcttttcgt accaggtggc ggtcggcaga acgacgtccg agtacatgct
     1261 cgtggtcgac attcggaagt cgatgttgac cattaggtcg agcttcccga cgggggcgtc
     1321 ctcgcgccag acgacgtcct tcggccgctc aatccccgcc gcctcttcat cggtgccgat
     1381 cacgccgtga agcgagccga ccaagtgctt caggaaatac tcatgcccct tgccgctcgc
     1441 accgagcacg ttcgagcgcc agaagaacat gttgcgcggc cagttgaccg gattgtccgg
     1501 gtcgaggccc ccgggcgtca gctcgccgga cttgaacgct tcggcgatcg cctgcgctgg
     1561 atcggcgccc gattccttga gcctgttgcc ccattcgatc gggttggcgt cgaattgcgg
     1621 tgccgacggg agccatccca tccgctcggc cttgacgttg tagtcgatca tgcttcccga
     1681 ccagtcgccc tccggcgcca gcggcgagag tatctccgac atcttcaggg attcgtatcg
     1741 ccactggtcg gtatgcgcgt agaagaaact ggtgccgttc atctgtcggg acgggcgcga
     1801 ccagtccgat gcgaacgcca acggcagcca gccggtctgc ggccgaagct tctcctgtcc
     1861 gacatagtgt gaccagcctc cgcccgactg gccgatgcag ccgcacatga tgagcagcga
     1921 gatgatcccg cgatagctca tatccatgtg gtaccagtgg ttgaggccgg ctccgaggat
     1981 caccatcgat cgaccgctgg tgacctcggc gttcgacgcg aactcgcgcg cgacctggat
     2041 gatcgcttgg cgaggaacgc cggtcacctt ttccgcccag gcgggcgtga acggcacgtc
     2101 gtcgtcatag ctcttcgcga cattgtcgcc gccgaagccg cgatcgactc cgtagttggc
     2161 gcagaacagg tcgaagacag tcgcgacgaa cgccttgctt ccatcggcga gcttcagctc
     2221 tttcaccggc acgttgcgga caaggatgtc gtcatgctcg gtcgccacga aatcgtgcgg
     2281 agcgtccgcg ccgaaatagg ggaaggccac gggctggatg gagtcattgt cggcagccag
     2341 gctgagctgt agccggacgc ctttgccgcc ctcctccacc tcctcgaggt tccacttgcc
     2401 gggatcgccc cagcgatggc cggcacttcc gacgggtatc actagcttgc cgctcaactc
     2461 gtcataggcc aggcacttcc agtcgggatt gttcgtcttc gcgggcttgc cgccgatgtc
     2521 cgccgggcgc atg
//
LOCUS       DASSGX010000603         2412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1629873, whole genome shotgun sequence.
ACCESSION   DASSGX010000603 DASSGX010000000
VERSION     DASSGX010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1629873"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            303..758
                     /locus_tag="VFI12_10965"
     CDS             303..758
                     /locus_tag="VFI12_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056975.1"
                     /translation="MADIREMLFTDAEINQQALANASALPYLMVAFARACGTSAEEVA
                     EFSGRIFAPGWARLAGAGAYDVLRIVALNLVCCGGEVERLNGDEDSAEARITGVPVQD
                     EADFFGITIDQTDQYSAVFTPIVASLGFNFSWRRDGAALVYSLQRSETD"
     gene            878..1945
                     /locus_tag="VFI12_10970"
     CDS             878..1945
                     /locus_tag="VFI12_10970"
                     /inference="COORDINATES: protein motif:HMM:NF015366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HET7056976.1"
                     /translation="MNVDRCARGVSRLLVAVLVASAALAAGLAPSAGSAFAQSSCSTD
                     YPSKGIQIMAPAAPGGGWDTTAREIQAALQSGVTDQNVEVFNVEGAGGTIGLAQLAAD
                     DKGDPYTLMVMGLVMIGAIQTNQAQVSLDDVTPIAELTGEFEVVVVPKDSKYQTLQDL
                     MADFKADPGGISWGGGSAGGTDHILVGLIAKDQGIDPTQINYIPYSGGGEALAAILGG
                     QVSAGVSGLSEWQDQIISGDLRVLAISGKSESSATPTASATPAVDPALAAIPTLKDQG
                     VNVELANWRGIVAPPGVSAEDQACIVEMITKMHDSEAWQASLAKYGWQDLFVTGDDFS
                     QFLQSERQRVEGILKDLGLVA"
     gene            2042..>2412
                     /locus_tag="VFI12_10975"
     CDS             2042..>2412
                     /locus_tag="VFI12_10975"
                     /inference="COORDINATES: protein motif:HMM:NF018979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HET7056977.1"
                     /translation="MEPNNRPQSNTDRLAELALAAGVVILGAIVLWQTRHIRVTPINS
                     RIGPRVIPYVVGSGLVVVGFWLAVEVLLGRTSRPVAGGEDSEDADPTLPTDWMTIGFI
                     AASLVAYLLLIERAGFIIASAV"
BASE COUNT          400 a          806 c          783 g          423 t
ORIGIN      
        1 tcggcgagct tagcccgttg atctaccggc aaatcgttct cgtttcgcga cattcggtcc
       61 tactccctgc tcggggcagt gacgtcgcca ccctcggcag agctcggcgc gcacgacttg
      121 gtcagcgtac aattctggca actctctcac ggcaatacca gtgcaaaacc aatcaccaga
      181 acccgggcat cccgcaagcc accggttgcc aatccccgca cgcgttttcc ttgacagtcg
      241 gtcacgctcc gcgagaatgg agagcagaat tgctatcgca gcgggacgca gaggaagagc
      301 cgttggctga catccgggag atgctcttta ccgatgccga gatcaaccag caggcgttgg
      361 ccaatgcctc ggcactgccg tacctgatgg tggccttcgc gcgtgcctgc ggcacgtcag
      421 cggaagaggt ggcggaattc tccggccgca tctttgcgcc gggttgggcc cggctggccg
      481 gcgccggcgc gtatgacgtg ctgcggatcg tcgctctcaa tctggtctgc tgcggcggcg
      541 aggtcgagcg gctcaacggc gacgaggaca gcgccgaggc gcgcatcacc ggagtcccgg
      601 tccaggacga agcggacttt ttcggcatca ccatcgacca gacggatcag tacagcgcgg
      661 tgtttacgcc gatcgtcgcg tcgctcggct tcaacttcag ttggcggcgg gacggcgcag
      721 cgctcgtcta ctcgctccaa cgctcagaga cagactgacc agactcgtca ccctcgcaca
      781 cccccgtttg taacctgccc tacccggggc ggtagttttc gcgtgccata ctctgggccg
      841 gttcgttgtt acaaactcaa tggggagggt tggctcgatg aacgtcgatc gttgtgctcg
      901 gggagtgtcg cgactgctgg tcgcggtgct cgtggcatca gcggcgcttg ccgccgggtt
      961 ggcgccgtcc gccgggagcg ccttcgcgca gtcctcatgc agtacggact atccctcgaa
     1021 gggcatccag atcatggccc cggccgcgcc cggcggcggg tgggacacga ccgcccgcga
     1081 gatccaggcc gcgctccaaa gcggcgtgac cgaccagaac gttgaggtct tcaacgtcga
     1141 gggcgccggc ggcacgatcg ggctcgccca gctcgctgct gacgacaagg gcgatccgta
     1201 caccctgatg gtgatgggtc tcgtcatgat cggcgcgatt cagaccaacc aggcgcaggt
     1261 cagccttgat gacgtcaccc cgatcgccga gctgaccggc gagttcgagg tcgtcgtcgt
     1321 cccgaaggat tccaagtatc agacgttgca ggatctgatg gccgacttca aggctgatcc
     1381 gggcgggatc tcatggggcg gcggctcggc cggcggcact gaccacatcc tggtcggcct
     1441 gatcgccaag gatcagggca tcgacccgac gcagatcaac tacatcccat actccggcgg
     1501 cggtgaagcc ctcgcggcga tcctcggcgg ccaggtctcg gccggcgtgt cgggcctgag
     1561 cgagtggcag gaccagatca tctccggcga cctgcgggtg ctggcgatct ccggcaagtc
     1621 cgaatcgtcc gcaacgccga ccgcatcggc caccccggcg gtggacccgg ccctggccgc
     1681 gatcccgacc ttgaaggacc agggcgtgaa tgtcgagctg gccaactggc gcggcattgt
     1741 cgccccgccc ggcgtttcgg ccgaggatca agcctgcatc gtggagatga ttacgaagat
     1801 gcacgactcc gaggcgtggc aggcgtcgct ggccaagtac ggctggcagg atctgttcgt
     1861 caccggcgac gacttcagcc agttcctgca aagcgagcga caacgcgtcg agggcatcct
     1921 caaggacctc ggcctcgtcg catagcgcgg atcacactga ccatggtcgc ggggagccat
     1981 acggcgtccc cgcgcctccg ttccccgctg acaatcccgt cccggccgca ggagtcctgg
     2041 cgtggagccg aacaaccgtc ctcaatcgaa cactgaccgg ctcgcggagc tggcactcgc
     2101 ggccggcgtc gtgatcctcg gcgctatcgt cctttggcag acgcgccata tccgggtcac
     2161 gccgatcaac tcgcggatcg ggccgcgggt catcccctac gtcgtcggct cggggctcgt
     2221 cgtcgtcgga ttctggctgg cggtcgaggt gctgctcggc cggacgtccc ggccggtcgc
     2281 cggcggcgag gacagcgaag acgccgatcc gacactgccg acggactgga tgacgatcgg
     2341 cttcattgcc gcctcgctcg tcgcctacct gcttctgatc gagcgggcag gcttcatcat
     2401 cgccagcgcc gt
//
LOCUS       DASSGX010000604         2146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1521501, whole genome shotgun sequence.
ACCESSION   DASSGX010000604 DASSGX010000000
VERSION     DASSGX010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1521501"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1019
                     /locus_tag="VFI12_10980"
     CDS             <1..1019
                     /locus_tag="VFI12_10980"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7056978.1"
                     /translation="RRTVLGALAAASGSAALTRLGVAQDASPATGEWTYTDVLGTTVT
                     LPEAPVRIVANIVTAAALWDLGIEPVGVFDWTVSNYPDGDHIAWGNIPADKVVNVGNA
                     DGNIEPEMLLTLDPDIVLTQTFDPTDPTLTNGVLPELLEQIDAIAPVLVVTDMAPTDV
                     LVDRLVQLGASLGANLEAPEVVTAREAYDAKIAEFQQVAAEKADLTNLFANFDTALIY
                     VGGPKDVSELIWLSALGLNFANADSELANEFWEELSVEQALLYPADVIFSDVYSVLLT
                     AEDLAAEPVFAAMPAVAAGQVGLWNRDFPVSYAGATAFLETILKTLRTAEKVTDASAA
                     TPSA"
     gene            1404..>2146
                     /locus_tag="VFI12_10985"
     CDS             1404..>2146
                     /locus_tag="VFI12_10985"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HET7056979.1"
                     /translation="MRDALLGFQPHFDLDLTTNARPEQILALGPGAGATSTYDIGAKF
                     GTIGLVFTDQDGNDPLSVEITTYRSEHYPDESRHPDVQFGADLIADLARRDFTINAMA
                     VNVMTGELIDPFDGQVDLAMATIRAVGDAEERFEEDPLRLLRAARFVSQLGFMIERDT
                     LDAMIETAPTIQRISRERIYGELTRLMTGTYASHALEVLSETGLLTLTMPELEPLSRE
                     TGDRGPVHREKDLWEHTKKVVDQAPQRPVV"
BASE COUNT          369 a          713 c          675 g          389 t
ORIGIN      
        1 cccgccgcac cgtgctgggc gcgctggccg ccgcatccgg ctcggctgcg ctcacccgac
       61 tcggtgtcgc gcaggatgcc agcccagcca ccggcgaatg gacctatacc gacgtccttg
      121 gcacgacggt tacgctgccg gaggcgcccg tgcgcatcgt cgccaatatc gttactgccg
      181 ccgcgctctg ggatctgggc atcgagccgg tcggtgtgtt cgattggaca gtgtccaact
      241 atcccgacgg cgaccacatc gcctggggca atattcccgc tgacaaggtc gtcaacgtgg
      301 gcaatgccga cggcaatatc gaaccggaga tgctgctgac gctcgatccc gatatcgtgc
      361 tgacccagac cttcgatccg accgacccca ccttgaccaa cggagtgctc cccgagctgc
      421 tcgaacagat cgatgcgatt gccccggtcc tggtcgtgac cgatatggcc ccgaccgatg
      481 ttctggtcga tcgtctggtc cagctcggcg catccctggg agccaatttg gaggcgccgg
      541 aggtcgtgac cgcgcgtgag gcgtacgacg ccaagatcgc agagttccag caggtcgcgg
      601 cggagaaagc cgatctgacc aatctcttcg ccaatttcga taccgcgctg atctacgtcg
      661 gtgggccaaa ggatgtctcc gagctgatct ggctcagtgc gctcgggttg aatttcgcca
      721 atgccgattc cgagctggcc aacgaattct gggaagagct gagcgtggag caggcgctgc
      781 tctatcctgc cgatgtgatc ttctcggatg tctatagtgt gttgctgacc gccgaggacc
      841 ttgcggccga gccagtcttc gccgccatgc cggccgttgc cgcgggacaa gtcggtctct
      901 ggaatcgcga cttcccggtc agctacgcgg gcgccaccgc cttcctggaa accatcctga
      961 aaaccctgcg caccgccgag aaggtcacgg acgcctcggc ggccactccg tcggcgtagg
     1021 gagcgaaccc ggtcgtaggg catgcacacc gcggcatgct tctacgaccg ggtgagcgcc
     1081 ggcccgggca tggcgcggag tccaggcatg gcacggtgtc catgccctac gttcggtgtg
     1141 cgccggcctc cgggctccgg attcggtact ccggacccga tcgatgcggt agactggccg
     1201 atcgttgccg gcgcggtgtg gccggccaga cgatcgtagg ggagggtgtc tctgccctct
     1261 cggttcccga cgagatgacc gcaacaagct ccgatcgacc gtcattggcg gccatgcttc
     1321 agcctcgaca cgcccaggtg gtcgaacggc tggccgcggc gttcgcggaa tcaggcttcg
     1381 agctctcgct ggtcggtggc atcgtgcgcg atgcgctgct gggattccaa ccgcatttcg
     1441 atctcgatct caccacgaat gcccggccgg aacagatcct cgcccttggg ccgggcgccg
     1501 gcgccacctc cacctacgat atcggcgcga agttcggcac catcggactg gttttcaccg
     1561 atcaggacgg caacgatccg ctctcggtcg agatcacgac ctaccgctcc gagcactatc
     1621 cggacgaatc ccgccatcct gacgtgcagt ttggcgccga cctgatcgcc gatctggccc
     1681 gccgtgattt caccatcaac gccatggccg tgaatgtgat gaccggcgag ctgatcgatc
     1741 ccttcgacgg gcaggtcgac ctggcgatgg cgaccatccg tgcggtgggc gacgccgagg
     1801 agcggttcga ggaagatccg ctgcggttgc tgcgggccgc tcgtttcgtc agccagctcg
     1861 gcttcatgat cgagcgcgac accctcgacg cgatgatcga aaccgcgccg accatccagc
     1921 gcatcagccg cgaacggatc tacggcgagc tgacccggct gatgaccggc acctatgcct
     1981 cgcacgccct cgaagtgctt agtgaaacag gcttgctgac cctgaccatg ccggaattgg
     2041 aaccgctttc gcgcgaaacc ggcgaccgcg gtcccgtgca tcgggaaaag gatctctggg
     2101 agcacaccaa gaaagtggtc gatcaggcgc cgcagcgacc ggtggt
//
LOCUS       DASSGX010000605         2888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1193456, whole genome shotgun sequence.
ACCESSION   DASSGX010000605 DASSGX010000000
VERSION     DASSGX010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1193456"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1768)
                     /locus_tag="VFI12_10990"
     CDS             complement(<1..1768)
                     /locus_tag="VFI12_10990"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HET7056980.1"
                     /translation="MRDLFRRKRPNRATETILSEQQNELLAAELAALEQLGAILDAYP
                     ATDDDKEALDDAAEQLTSLFLLVVVGEFNSGKSAFLNALVGARIMPEGVTPTTSVINL
                     LQYGETQSETMLPDGIVARTFPADFLDEITLVDTPGTNAIVREHEALTQRFVPRADLV
                     LFVTSADRPFTESEHLFMADIREWGKKITIVINKIDMLRTEEDRTRVVEFVTENIERL
                     LGFRPEVFPVSALLAQQAKDIGQTNPAESQRLWQESRFGALERYILESLDEESRIRLK
                     LLSPLGIAEHLTGRYMKATNDRLAILADDVDTIRTIDRQLQLYSEDMRRQFAYHLTRI
                     ENIIGRMTARGDEFFEETIRIGRVFDLLNSDKIRSEFERKVVGDTEKQIDVTIHELID
                     WMVEQDLRTWEAVQEYVDRRRLTQYEEEMIGEVGAQFKYDRRSLLESVSHRAKEEVER
                     YDPEEHAKELSLSVRSAVAQVAVAEAGAVGLGALVVAAASTVAVDVTGILAASLVAGL
                     GFFILPRKRKQSRAEFRRRSEELEERLVAVMSEQFEHELARSIARIQDAIAPYTRFVR
                     DQQAKFTGIEENLTSVANELRSL"
     gene            complement(1823..2446)
                     /locus_tag="VFI12_10995"
     CDS             complement(1823..2446)
                     /locus_tag="VFI12_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET7056981.1"
                     /translation="MHRVAMNELLSELFSSKVRAAVLGHMLPRPQVALSLTEFSRLLG
                     LPISSLQHECYKLERIGIIKGKREGNSRRYRVNGQCRILPELTALVVAALGQEAAVRA
                     TLADVPGLEAAFIGRALPIDDGAEISATPIPLVLIGEIPLEEIDSAVDRVSRLLDLPA
                     TKFEAVFYRPGDWRARLDQNSQYAVWLVSGPRTDLVGNPMTLPNGPA"
     gene            complement(2534..>2888)
                     /locus_tag="VFI12_11000"
     CDS             complement(2534..>2888)
                     /locus_tag="VFI12_11000"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET7056982.1"
                     /translation="DVMTTRVVTTKPDRSIWDAGRVLLDKGFGGMPVVDSTGKVVGML
                     SGFDVISKAGTTVGEIMSRGVVAVKPTDSIDDVIHLMGLHGIRRVPVFDGDRLVGIIS
                     RSDLLAHVIDQFEHE"
BASE COUNT          490 a          933 c          938 g          527 t
ORIGIN      
        1 ggagggagcg caactcgttg gcgacgctgg tcaggttctc ctcgatcccg gtgaatttcg
       61 cctgctgatc ccgcacgaac cgcgtgtagg gcgcaatcgc gtcctggatt cgggcgatcg
      121 agcgagccag ctcgtgctcg aactgctcgc tcatgactgc gaccagccgc tcctccagct
      181 cctcggaccg ccgccggaat tccgcgcggg actgcttccg cttgcggggc aggatgaaga
      241 agccgagccc ggctacgagg ctcgccgcga ggatgccggt cacatcgacc gcgaccgtgc
      301 tagccgccgc gacaaccagc gcgccgagac cgaccgcgcc cgcctcggcc actgcgactt
      361 gtgccaccgc gctgcggacg ctcagcgata gctccttggc atgctcctcg gggtcgtacc
      421 gctcgacctc ttccttggcc cggtgcgaga ccgattcgag caggctgcgg cggtcgtatt
      481 tgaactgcgc gccgacctcc ccgatcatct cctcctcgta ctgcgtcagg cggcggcgat
      541 cgacatattc ctggaccgct tcccaggtgc gcagatcctg ctcgaccatc cagtcgatca
      601 gctcgtggat cgtgacgtcg atttgcttct cggtgtcgcc gacgaccttc cgctcgaact
      661 cggagcggat cttgtcggag ttgagcaggt cgaaaacgcg gccgatccgg atcgtctcct
      721 cgaagaactc gtcgccccgg gccgtcatgc ggccgatgat gttctcgatc cgggtcaggt
      781 gataggcgaa ttgccgccgc atgtcctcag agtagagctg gagctggcgg tcgatcgtgc
      841 ggatcgtatc gacgtcgtcc gcgaggatcg cgaggcggtc gttcgtcgcc ttcatgtagc
      901 ggccggtcag atgctcggcg atcccgagcg ggctgagcag cttgaggcgg atccggctct
      961 cctcatcgag cgactcgagg atgtaccgct ccaacgcgcc gaatcggctc tcctgccaaa
     1021 gccgctggct ctcggccggg ttggtctggc cgatgtcctt ggcctgctgg gcgagcagcg
     1081 ccgagaccgg gaagacctcc ggacggaagc cgagcagccg ctcgatgttc tcggtcacga
     1141 actcgacgac gcgggtacga tcctcctctg tccggagcat gtcgatcttg ttgatcacga
     1201 tcgtgatctt cttgccccac tcgcggatat ccgccatgaa caggtgctcg ctctcggtga
     1261 acgggcgatc ggcggaggtg acgaagagga cgaggtcggc ccggggtacg aaccgctgcg
     1321 tcaacgcctc atgctcgcga acgattgcgt tcgtgccggg cgtatcgacc agcgtgatct
     1381 cgtccaggaa atcggccggg aaggtgcggg cgacgatgcc gtcgggcagc atcgtctcgg
     1441 actgcgtctc gccgtactgg agcaggttga tgacggaggt tgtcggcgtt acgccctcgg
     1501 gcatgatccg ggcgccgacc agcgcgttca ggaaggccga tttgccgctg ttgaactcgc
     1561 cgaccacgac cagcagaaag agcgacgtga gctgctcggc tgcgtcgtcc agcgcctcct
     1621 tgtcgtcgtc ggtggctggg taggcatcga ggatcgcgcc cagctgttcg agcgcagcca
     1681 gctccgcggc caacagttcg ttctgctgct cgctgagaat ggtctcagtg gctcgattcg
     1741 gtcgcttgcg gcgaaacagg tcacgcatga gcagggttcc aatcccggaa taggcgacgc
     1801 gtcagtgcgc cggcgtctga gtttaggccg gaccgttggg aagcgtcatg ggattgccaa
     1861 ccaaatccgt gcgtggtccc gaaaccagcc agaccgcata ttgcgagttc tgatccagcc
     1921 gcgcccgcca atcgccgggc cggtagaaga cagcctcgaa cttcgttgcc ggcagatcga
     1981 gcaggcgaga gacgcggtca accgcgctat cgatctcctc gagcgggatc tcgccgatca
     2041 ggacgagcgg gatcggtgtg gcgctgattt ccgcgccgtc gtcgatcggc agggcacggc
     2101 cgatgaaggc ggcctccagc cccggcacgt ccgcaagggt cgcgcggact gccgcctcct
     2161 gcccgagtgc ggcgacgacc agggcggtca gctccggcag gatccggcac tgcccgttga
     2221 cccggtagcg ccgggagttg ccctcgcgct tgcccttgat gatcccgatt cgctccagct
     2281 tgtagcactc gtgctgaagg gagctgatcg gcaggccgag tagccgcgag aactcggtca
     2341 gggagagcgc gacctgcggc cggggcaaca tgtgcccgag caccgccgcg cggactttcg
     2401 acgagaacag ttctgacagc aactcgttca tggcgacccg atgcacaacc cagcaatccg
     2461 gactgacagg ttacggagcg aacgtatcat acccggattt tggtaagtcg cacacgcggg
     2521 gctacgaacg tccttattcg tgctcgaatt ggtcgatgac gtgcgcgagg aggtcggagc
     2581 ggctgatgat gccaacgagg cgatcgccgt caaagaccgg cacgcggcgg atgccatgca
     2641 gccccatcag atggatgacg tcatcgatcg agtcagtcgg ctttaccgcc acgacaccgc
     2701 ggctcatgat ctcgccgacc gtcgttccgg ccttgctgat cacatcgaaa ccgctcagca
     2761 tgccgaccac tttgccagtc gagtcgacaa ccggcatccc gccgaagccc ttgtcgagca
     2821 ggacgcgccc ggcatcccag atcgatcggt ccggtttggt cgtgacgacc ctggtggtca
     2881 tgacatcg
//
LOCUS       DASSGX010000606         6114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_868266, whole genome shotgun sequence.
ACCESSION   DASSGX010000606 DASSGX010000000
VERSION     DASSGX010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6114
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868266"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..1499
                     /locus_tag="VFI12_11005"
     CDS             222..1499
                     /locus_tag="VFI12_11005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HET7056983.1"
                     /translation="MSLQRTVSRHLPQTRETWMRLVILSNLIFWAAHAIHELWIRGLF
                     VVLGGDWASFWGATQAFERIKPAAGYHISAIAAATRPLSVFSHDGARGARIGPAPYPP
                     IFLDLFSLFTALPPVVGYLLWTAISALIALVVFERVATYARVRSPWTIALLLLSSFPV
                     MMALFVGQVVSILLICILFAVLDLQAGRELRAGFWIGLLALKPQYAPCLVLVLLLKRR
                     WSALAGVGLGGSAVLVASLITGGLSGVVAYGRELVTSYPSFAGSAGVDPRGQVSWRSM
                     VAMLLPGLGTGWGLALVVVLSILTLLTLPFIWRGPWNPQSADFTPKFMATLAVTLLVA
                     YQSQPHGAALLLIPGVLVVRQSRARAAFRRLFAAAIAVAPWLGLVSALALGNLWLVSL
                     AISGLLVSVIILTLRPAGRVPAPALAGMRHSVA"
     gene            1563..2540
                     /locus_tag="VFI12_11010"
     CDS             1563..2540
                     /locus_tag="VFI12_11010"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET7056984.1"
                     /translation="MSLTPSGTHPWVAEARDRIRFGISIFPQPVDWRQFIDIVQRAEA
                     DGMDSYWSYDHPQSRADCWTALSALAACTSTIRLGTAIDCIYYRSPYMLARQAADVDR
                     LSGGRLVLGIGIGDNVPEFHRMGLEFPPTKVRQQGMVETITILRGLWSGTPFHFRGEQ
                     WSASCDVAYPFLGPVQQPWVPIMIAGGGEKVTLRQVAQYADASNMGAHEWIGSAVSSA
                     DIDRKFGKLREYLSEHGRSPESVLRSQFTMPLVMAETQAELDRKLAGMPQDVLEWCGD
                     ALFAGTPDETIAFYEDLVAAGFQYFIANILDGDYSTIELMGKYLVPAFR"
     gene            complement(2602..4092)
                     /locus_tag="VFI12_11015"
     CDS             complement(2602..4092)
                     /locus_tag="VFI12_11015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056985.1"
                     /translation="MAPVQSRRRFWFGRLGSFSVVLLMLLSGAITGRGAVHAQDATGG
                     STTADVTWGTVQITTRDNFTDDVLTGACYIVSTDGLGGSQGCDFQDGLDGVTTIRAIA
                     GAITIYTANPPSYHYYGEPITGLSLATDETKTLTIRHQAGGGDVIVKMFTPEGTAALN
                     GCAQVAVNNNGVPGDLKSGSVCDWMDGSSNGVLRLNGVSQYPTASALVVMSYTPPGFK
                     TAAPEAVTIQNGATKRAYFHLSRLALNPYCTAGPTSGKVGTSVKVSCKGFAQYQVVYV
                     KWDGRSSATTTTYTDQDGRFTTHLTVPAAVAGTHRIVIASDSHRAPTKYFTVIPSLSV
                     SPNSGTADTSVRATLRGFAKGEIINIRVQGQRRIRATATASSTGSASVLFTVPNGSTT
                     DIIIRAEGSAGNVATARFHRTEIATAEEPAPAAEATSTPEPTGTPTVAPTETPTAPPV
                     DAGTPEPPAPTETAITPPPDAPTDTPSATEPSVDPPAQEVPATPTE"
     gene            complement(4324..>6114)
                     /locus_tag="VFI12_11020"
     CDS             complement(4324..>6114)
                     /locus_tag="VFI12_11020"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HET7056986.1"
                     /translation="DGLPLAIELAAAKAASLGLDTLAERLTNRRQVLDLSDGATDPRH
                     HSLRSAIAWSYELLEPEAQVALNRLSVFSGGFSQSSVERMLGGSPVLRRYSPNRSHEF
                     EWVERDFTQISASVQIRPLSIESRIALERLLDANWIQVSAGADGRPRYQMLESIRDFG
                     LEQLRATGEYGHVRTAHAVAMLQFMDMAGYELWYTAGADSFREQRVEEQGNLRLALGW
                     ACDPANDLAMLAGRLICSSWFHFQLSGQISEGRMWLERVLALPNAPLWSRLQVMNALS
                     FSSWTQGDADRADQLSQAVIREWGRSAPYQWLGIAHFNAGLVAWRRGDFAAMTSSLLT
                     GKQILHDAGDLNGEGFCNLALGVLARFSGQYDDARQLFDGALALHTQAQHEWGAATSR
                     YLAGEAARDEGNIPGAAGLIADGLRRYRDQSDIFGSGACVAALAVFAAERHELARAAR
                     LFGAAFAMCEYAGVLLPPVDLERYQQTAAAVATQVPASEFQRGRSWSIERATDEALAV
                     AADVQAGRVPGPIDDPLRTILTADQRDAVNLLCDGLSVDEIATRLFRDPNTIYDRLAR
                     ARQRLGVSSNRKLVSAVTALRAGLGTTDGS"
BASE COUNT          974 a         1955 c         2022 g         1163 t
ORIGIN      
        1 cgcgccagcg catcaaaccg ctcactcttc acgattgact cctgaccaaa cgcgatccgc
       61 actggcctgt tggggcaggc cgatcatagc acccacctga gcgtgcggcg ccgaaatagc
      121 ggttcgtcgc ttaacgagag gttcatgcac gggcggtata agagaaaacg ggcgggagtc
      181 gccctttgcg tctccgagtg atttcaggtc ggttccgcag catgtcgctt cagcgcaccg
      241 tttctcgcca tctgccgcag actcgcgaga cctggatgcg actggtgatc ctgtccaatc
      301 tcatcttctg ggcggcccac gccattcacg agctctggat ccgtggcctc ttcgtcgtgc
      361 tcggtggaga ttgggcgtca ttctggggcg ccacgcaggc gttcgagcgc atcaagcccg
      421 ccgccggcta ccacatctcg gcgatcgccg ccgcgacacg cccgctgtca gtcttcagcc
      481 atgacggcgc tcgtggcgcc cggatcggcc cagccccgta tccgccgata tttctcgacc
      541 tcttctccct cttcaccgcg ctcccaccgg tggtcgggta cctcctgtgg acggcgattt
      601 cggccctgat cgcgctggtc gtattcgagc gcgtggccac gtacgcccgc gtgcgctcgc
      661 catggacgat cgcactgctc ctcctgagct cgttcccggt gatgatggcc ctgttcgtcg
      721 gccaggtcgt ctcgatcttg ctgatctgca tcctgttcgc cgtgctcgat cttcaggcgg
      781 gacgggagct gagggccggc ttctggattg gcctgctcgc gctcaagccg caatacgcgc
      841 cgtgtctcgt gctcgtcctg ctcctgaagc ggcgctggtc ggcgcttgcc ggggttggcc
      901 tcggcggtag cgcggttctc gttgcgtcgc tgatcaccgg cggattgagc ggcgtggttg
      961 cctatggccg cgagctggtg acgtcatatc cgtcgtttgc ggggtctgcc ggggtggatc
     1021 cgcgggggca ggtgagctgg agatcgatgg tcgccatgct gctgccggga ctcgggacgg
     1081 gctggggcct ggcgctggtc gtcgtgcttt cgatcctgac gctcctgacc ctgccgttca
     1141 tctggcgcgg cccgtggaac ccgcaatccg ccgacttcac cccgaaattc atggcgaccc
     1201 tggcggtcac gctcctggtg gcgtaccaga gccaaccgca tggggcggcg ctcttgctga
     1261 tcccgggcgt gctggtcgtg cgtcaatcgc gggcccgggc ggcgttccgg cggctctttg
     1321 cggcggcgat agcggttgcg ccgtggctcg gtctcgtctc ggccctggcg ctgggcaatc
     1381 tgtggctggt ctcgctcgcg atctccggtc tgctcgtgtc ggtcatcata ctgacgctca
     1441 ggcccgctgg cagggtcccc gctccggcac tggccggaat gcggcattcc gttgcgtaga
     1501 atgccgcgag tacggctcga tccaacctga tcgcaaagtg agcgtcaatg ccgaatccag
     1561 aggtgtcctt gaccccgagt gggacccacc cgtgggttgc cgaggcgcgc gatcgcatcc
     1621 gtttcgggat cagcatcttt ccgcagccgg tcgattggcg ccagttcatc gacatcgtcc
     1681 agcgggccga ggcggacggc atggactcct actggagtta cgatcatccg cagtcccgcg
     1741 ccgactgctg gaccgctctg tcggccctcg cggcctgcac gagcacgatc cggctcggca
     1801 cggcgatcga ctgcatctac taccgctcgc cctacatgct cgcccggcag gcggccgatg
     1861 tcgatcgtct cagcggcggc cggctggtcc tcggcatcgg gatcggtgac aacgtgcccg
     1921 aattccaccg gatgggactc gagttcccgc cgacgaaggt gcgtcagcag gggatggtcg
     1981 agacgatcac gatcctgcgc ggactctggt cgggcacgcc gttccatttc cggggcgagc
     2041 agtggtccgc ctcgtgcgac gtcgcgtacc cgtttctggg ccccgtacag cagccgtggg
     2101 tgccgatcat gatcgccggc ggcggcgaga aggtcacgct ccggcaggtg gcgcagtatg
     2161 ccgatgcctc gaacatgggt gcccacgaat ggatcggcag cgcggtcagc agcgccgaca
     2221 tcgaccgcaa attcggcaag ctgcgcgagt acctgtccga gcacgggcgg tcccccgagt
     2281 cggtcttgcg gagccagttc acgatgccgc tggttatggc ggagacgcag gccgagctcg
     2341 accgcaagct ggcggggatg ccgcaggacg tgctcgagtg gtgcggcgac gccctcttcg
     2401 cgggcacgcc ggacgagacg atcgccttct atgaggatct ggtcgcggcc ggctttcagt
     2461 acttcatcgc caacatcctt gacggcgact attcgacgat cgagttgatg ggcaagtacc
     2521 tggtccccgc ctttcgataa cgggaacgag ggacctggcg gtcccccgtt cccgtggatg
     2581 tgtgcccgga gcgtcggcgt gctactccgt tggtgtggcg gggacctcct gggcaggtgg
     2641 gtccacgctg ggctctgtgg cggacggagt atccgtcggc gcgtcgggcg ggggtgtgat
     2701 tgcggtctcg gtcggcgccg ggggctccgg agtgccggcg tccaccggcg gcgcggtcgg
     2761 cgtttcagtc ggcgcaaccg tcggcgtccc ggtcggttcc ggcgtgctgg tggcctccgc
     2821 cgctggcgcg ggctcctcag ccgtggcgat ttcggtccga tggaagcggg cggttgcgac
     2881 gtttccggcg ctgccttctg cccggatgat gatgtcggtg gtgctgccat tcggcaccgt
     2941 gaagaggacc gatgcgctgc ccgtggaact ggccgtggcg gtggcccgga tccggcgctg
     3001 gccctgaacc cggatattga tgatctcgcc cttggcaaac ccgcggagtg tggcccggac
     3061 agaggtgtcc gcggtcccgc tgttcggact cacggagagc gacgggatca cagtgaagta
     3121 cttggttggc gcgcgatggc tgtcactcgc gatcacgatg cggtgcgtcc cggcgactgc
     3181 ggctgggacg gtcaaatgag tcgtgaatcg tccgtcctgg tccgtgtagg tcgtcgtagt
     3241 tgcgctcgat cgaccatccc acttgacata gacaacttga tattgcgcga agcccttgca
     3301 ggagacctta accgaggtgc cgaccttccc ggaggtaggg cccgccgtgc aatacgggtt
     3361 gagggccaac cgggagaggt ggaagtaggc gcgtttggtc gcaccattct ggatcgtgac
     3421 cgcctcaggg gcagcggtct tgaagccagg tggtgtgtac gacatgacaa ccagggccga
     3481 ggcggtcggg tactgagaaa ccccgttgag tcggagcacg ccgttgctcg aaccgtccat
     3541 ccagtcgcac actgatccgg atttcagatc acccggaaca ccattgttat tgactgcgac
     3601 ctgtgcgcat ccattgagcg ccgcggtgcc ttctggtgtg aacattttga cgattacgtc
     3661 accgccgcct gcctggtggc ggatagtgag tgtcttcgtc tcatcagtcg cgaggctaag
     3721 gcccgtgatt ggctcgccgt aatagtggta cgagggcgga ttcgcggtgt aaatcgtgat
     3781 agccccagcg atggctcgga tggtcgtgac tccgtccaat ccgtcctgga agtcgcagcc
     3841 ttgggagccc ccgagtccat cggtggagac gatgtagcag gcccccgtga ggacgtcgtc
     3901 ggtgaaattg tcgcgtgtgg tgatctggac ggtgccccac gtgacatctg cagtggtgct
     3961 tccgccggta gcgtcctggg catgaacggc gccgcggcca gtgatcgcgc cgctaagcag
     4021 catcagtagc acgactgaga acgaaccaag gcgtccgaac cagaacctgc gtcgcgactg
     4081 tacgggtgcc atcgcaacct cccacatccc ttgccggcag gcgtggccac cccggcccgc
     4141 cccggcgatc cctctactcc gcccacgctg aacccggccg atggggtcgc gggtacgccc
     4201 gcgccgagtc aattcgacgc cgaccagaga taccctcggc aagaacggtt caatttgtgg
     4261 actagcagaa tagtaggaga taagtaccaa aaggcaacac ggaaaaatcc cgtattttgc
     4321 gagtcaggat ccgtccgttg tgcctaagcc cgcccgcagg gccgtgactg cgctcacgag
     4381 cttccggttc gaactgaccc caaggcgctg gcgagccctg gccaggcgat cgtagatggt
     4441 gttcggatcg cggaagaggc gggtggcgat ctcgtctacc gacagaccat cgcagagcaa
     4501 attgacggcg tcccgctggt ccgccgtcag tatggtccgc aacgggtcgt cgatcggacc
     4561 gggcacgcgc ccggcctgga cgtccgccgc cacggcgagc gcctcgtccg tcgctcgttc
     4621 aatcgaccag cttcggcctc gctggaattc gctggccggg acctgggtcg cgacggccgc
     4681 ggcagtctgc tggtaccgtt ccaggtcgac cggcgggagg agcacaccag catattcgca
     4741 catcgcgaat gctgcgccga agagccgggc ggcacgcgcc aattcgtgcc gctcggctgc
     4801 aaagaccgcg agtgccgcaa cgcacgcgcc ggagccgaaa atgtcgctct ggtcccggta
     4861 gcgacgcaac ccgtcagcga tcaagccggc ggcccccggg atattgccct catcacgggc
     4921 ggcctcgcca gcgaggtagc gtgacgtggc cgcaccccat tcgtgctggg cctgcgtgtg
     4981 cagcgcgagc gcaccgtcga agagttgacg ggcgtcgtca tattgaccgc tgaaccgcgc
     5041 cagcacgccg agcgcaagat tgcagaatcc ttctccgttc aggtcaccag catcgtggag
     5101 tatctgctta cccgtcagga gagacgaggt catggcagcg aagtcgcctc gccgccaggc
     5161 gacaagccct gcgttgaaat gggcaatgcc taaccattgg tagggtgccg accgtcccca
     5221 ctcacggatg acggcctgcg acagttgatc cgcccggtcg gcatcgccct gggtccagga
     5281 agagaatgag agtgcgttca tcacctgcag ccgcgaccag agcggcgcgt tggggagcgc
     5341 gagcacgcgt tcgagccaca ttcttccctc actgatttgg ccactgagct ggaaatggaa
     5401 ccaggacgag cagatcagcc ttccggcaag catcgccaga tcgtttgcgg gatcgcatgc
     5461 ccatccgagg gccagccgca ggttgccctg ctcctccacg cgttgctcgc ggaacgagtc
     5521 cgcgcctgcc gtgtaccaga gttcgtatcc cgccatatcc atgaactgca gcatcgcgac
     5581 agcgtgggcg gtgcgtacgt gcccgtattc gcccgtcgcg cggagttgct ccaggccaaa
     5641 atcgcggatc gattcgagca tctggtagcg cggtcggcca tcagcaccgg cggagacctg
     5701 aatccagttc gcgtcgagca atcgctccag tgcaatgcga ctctcgatcg acaggggacg
     5761 gatttggacc gatgcgctga tttgggtgaa gtcccgttca acccactcga attcgtgact
     5821 tctgttcggc gaatatcgcc tgagtaccgg tgatcctcca agcatccgct caaccgacga
     5881 ctggctgaat ccgccgctga agactgagag ccggttcagc gcgacctgtg cttccggctc
     5941 cagtagctcg taggaccagg cgatagccga ccgaagcgaa tggtgtcgtg ggtcggttgc
     6001 gccgtcgctc aagtcgagca cctggcgccg gttggtcaaa cgctcggcca gagtgtcgag
     6061 gccaagggat gcggccttgg cagctgccag ctcgatcgcc agcggtagcc catc
//
LOCUS       DASSGX010000607         2445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1411924, whole genome shotgun sequence.
ACCESSION   DASSGX010000607 DASSGX010000000
VERSION     DASSGX010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1411924"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(208..972)
                     /locus_tag="VFI12_11025"
     CDS             complement(208..972)
                     /locus_tag="VFI12_11025"
                     /inference="COORDINATES: protein motif:HMM:NF016024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rad52/Rad22 family DNA repair protein"
                     /protein_id="HET7056987.1"
                     /translation="MTASKATEITNKLMEPFPVDVIEEKNGLLYIPHEIIRERLISAT
                     DNQFDWSLDQILFRDDGVTRRANDRYTGEPKRPLSMIVVGTLTIPGLGSRAGIGAHPL
                     DEGAGEDAAYKSAESDALKRAAMAFGVGLHQLYIETGEAKRVQKPRNGQVRAMSRPAQ
                     ERAPEPRKPAPSEKPVTDKQFGSDVRQAMAARDATAFRKLVDDANEHVGRWVALVQAA
                     ESPQALDWIKRQIERKGVNNDILAHEIRKREEQIAA"
     gene            1394..>2445
                     /locus_tag="VFI12_11030"
     CDS             1394..>2445
                     /locus_tag="VFI12_11030"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET7056988.1"
                     /translation="MSRSDQSPLAALVADLSAGRISRRSFIYGAGSFGLSAGLAGAIA
                     EGTLGTASAAPGTQGAIRSISLQDGGKTLVVAITQTTVQLDPAIAGSNGYGDIIPINE
                     NLYEGLTRYKNGTNEIEPALAESWTNSADGLSYVFKLRSGVTFHDGTPLDAKAVATNF
                     VRQLDENDPLHDPGMVYAGVILQDVSKVEATGDLEVTLTLGRPIKLVPANLAVFAAGI
                     VSPTALQTYKDDYSTHAAGTGPFKLDHWTKDVELVFVANDQYWGGRPKLDRVIWRTIA
                     DDTVKLSELMTGGVDVANQIDFKDIETVQSDADLQIVSGPFWNVQFLGVNAAVKPFDS
                     LQVRQALQYAINKQNIA"
BASE COUNT          403 a          773 c          809 g          460 t
ORIGIN      
        1 ggggctaccg caccgagcta cgtctccgga ggccgatccc cggccagctt cggcggattt
       61 cgggcgccga ggcgatggtc gggaaggggt gtcgcattgc catgcgacgc tacagatcgg
      121 gcgtgaaaaa gggcggagcg ccgccagcgc cccgcccttt gctcattacc gacgactgac
      181 gtccgaagtc cgatatccgc cgacttgcta tgcggcgatt tgctcttcgc gcttgcggat
      241 ctcgtgggcg aggatgtcgt tgttgacgcc cttgcgctcg atctggcgct tgatccaatc
      301 cagcgcctgc ggcgactccg ctgcctggac gagggcgacc cagcggccga cgtgctcgtt
      361 ggcgtcgtcg accagcttgc ggaaggcggt cgcgtcgcgc gcggccattg cctgccgtac
      421 atccgacccg aattgcttgt cggtcaccgg cttctcgctc ggggcgggct tgcgcggctc
      481 gggcgcgcgc tcctgggcgg ggcgggacat cgcccggacc tggccgttgc gcggcttctg
      541 gactcgcttg gcctcgccgg tctcgatgta cagctggtgc aggccgacgc cgaaggccat
      601 cgccgcccgc ttcagggcat cggactcggc cgacttgtag gccgcgtctt ccccggcgcc
      661 ctcatcgagg gggtgcgcgc cgatgccggc ccggctgccg agacccggaa tggtgagcgt
      721 cccgaccacg atcattgaaa gcggccgctt gggctcgccg gtgtagcgat cgttggcccg
      781 ccgggtgacg ccgtcatccc ggaacaggat ctgatcgagg ctccagtcga actggttgtc
      841 ggtggccgaa atcagacgct cgcggatgat ttcgtgcggg atgtagagga gaccgttctt
      901 ctcctcgatc acatcgaccg ggaacggctc catcagcttg ttcgtgattt ccgttgcctt
      961 gctggcggtc atgtaccttc acctcgcggg tgatccggcg gaccgcggcg ggccgccaga
     1021 gcaaatcaga gatgccgggt aatcgtgcgt cattgccgcg atacccggct cggcgatcgg
     1081 ggccggtgcc cctgaacgca tgttctattt tactttacgt tggcgttgac cgcaagacat
     1141 agagagtcga gaaggcgagg agtcgaggag gcgagaagcc ggaaaatgtg gcgaatttgt
     1201 gcggtgacta cacctgtctg tgggctgctg cggtagggtg aggatggtgc ttgggcatgg
     1261 gtgcgagaga cgagagtcca atcttgataa agaactctat aattacgtac ggcccttccg
     1321 ccgtgagccg gctgcgcgcg gggttcgtcc ggcgctggcc ttgtccctga agctagctgg
     1381 aggagtttgc gtcatgtccc ggtccgatca atcgcccctt gctgcgctcg ttgcggatct
     1441 ctccgccggg cgcatttccc gtcgttcctt catatacggc gcgggctcgt tcggtctctc
     1501 ggctggactc gcgggagcca ttgctgaggg gacgctcggg acggcatcgg ctgcgcccgg
     1561 gacgcagggc gcgatccgct ccatctcgct gcaggatggc ggcaagacgc tggtcgtcgc
     1621 gatcacccag acaacggtcc agctcgaccc ggcaattgcc gggtccaatg gctacggcga
     1681 catcatcccg atcaatgaga acctctacga ggggttgacc cggtacaaga acgggaccaa
     1741 cgagatcgag ccggccctgg ccgagagctg gacgaactcc gccgacgggc tctcctatgt
     1801 cttcaaactc cgctccggcg tcacctttca cgacggtact ccgctcgatg ccaaggcggt
     1861 cgcgacgaac ttcgttcgcc agctcgacga gaacgacccg ctgcacgacc cgggcatggt
     1921 ctacgccggc gtcatcctgc aggatgtgtc caaggtcgag gcgaccggcg atctcgaggt
     1981 aaccctcact ctcggccggc cgatcaaact cgtcccggcc aatttggctg tcttcgcggc
     2041 cggcattgtc agcccgaccg cgctccagac ctacaaggac gactacagca cccacgcggc
     2101 cgggaccggt ccgttcaagc tcgaccactg gacgaaggac gtcgagctcg ttttcgtcgc
     2161 caacgaccag tattggggtg ggcggccgaa gctggaccgc gtgatctggc gcacgatcgc
     2221 cgatgacacg gtcaagctct ccgagctgat gaccggcggc gtcgatgtcg ccaaccagat
     2281 cgacttcaag gacatcgaaa cggtccagag cgacgcggat ttgcagatcg tcagtgggcc
     2341 gttctggaat gtgcagttcc tgggcgtgaa cgcggcggtg aagccgttcg acagcctgca
     2401 ggtccgccag gcgctgcagt acgcgatcaa caagcagaac atcgc
//
LOCUS       DASSGX010000608         2591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_649063, whole genome shotgun sequence.
ACCESSION   DASSGX010000608 DASSGX010000000
VERSION     DASSGX010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649063"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VFI12_11035"
     CDS             complement(<1..429)
                     /locus_tag="VFI12_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056989.1"
                     /translation="MALFRHVPHALTLSPALIVALMALVLSVAGNAAAALIIMSNTQV
                     GAGTIAGHHPPSGDHANLIAGSVNGIDIDNDSITGADVNEETLRGSAHKLYFFDSEST
                     DPARTLAIVGPWTVKAECIHQDVPNSQVLELLVNGPGEAQV"
     gene            863..1402
                     /locus_tag="VFI12_11040"
     CDS             863..1402
                     /locus_tag="VFI12_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056990.1"
                     /translation="MSTGAWLFCGIVAAALIAPAGVYAAVNSHVAIGNVGNATTATVD
                     AEHQLLTAVVAPSDIVRISGAANVASNGCSVIYTPPTGKALVLVNVTVELEIQPGGTF
                     VADVISSRKDCGAPVDWFETTVDRHEEQHTYPAGLPLARLSLGTGYASGIWATGYLIP
                     ASQLPATIAPSGKLPPFHR"
     gene            1616..1951
                     /locus_tag="VFI12_11045"
     CDS             1616..1951
                     /locus_tag="VFI12_11045"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HET7056991.1"
                     /translation="MTDTKQRTRVATYERVSTEDRRERETIRTQTEQLERRLSLEEVE
                     MVARYVDDGISGCRRRFREREGGGQLLHDAEAGRFQELWVYHTDRLARNLSDMAATGR
                     CTKSSVSPW"
     gene            1969..>2591
                     /locus_tag="VFI12_11050"
     CDS             1969..>2591
                     /locus_tag="VFI12_11050"
                     /inference="COORDINATES: protein motif:HMM:NF019149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HET7056992.1"
                     /translation="MNPFLFDLIAVMAQNERRTLLRRSADGIARAIGEGRYVGGIVPF
                     GYRVVGEKAKARLEPHTAHLRSDQSAADIVIQMYRWVGLEGWSCRRVAGTLNLHSVPT
                     RYVRDGRSVKRGERRERTQGIWRDGRVSNLLVSPIYRGESTFGKRSEKRDRSVLRGSV
                     EPLVSQELWQAAQETLARNRVCAKNTRRTDLRRGNLHCAECGLSYVGS"
BASE COUNT          446 a          826 c          891 g          428 t
ORIGIN      
        1 gacctgcgct tctcccggac cgttgacgag tagttccagc acctgggagt tcgggacatc
       61 ctgatggatg cattccgcct tgacggtcca tggcccaaca atcgccaggg tccgtgcggg
      121 gtccgtcgac tcggagtcga agaagtagag cttgtgcgcg ctgcctcgga gggtctcctc
      181 gttgacgtcg gctccggtga ttgaatcgtt gtcgatatct atcccgttca ccgacccggc
      241 gatcaggttg gcatggtcgc ctgacggcgg atgatgaccg gcgatcgtgc ccgcaccgac
      301 ctgagtgttg gacatgatga ttaacgcggc ggcggcattg ccggccaccg agagaacgag
      361 cgccatcagc gcaacgatga gcgcgggcga cagcgtcagg gcgtgaggca catggcggaa
      421 gagagccaca gcgcgattcc tccccgcggg ctcgcgtggc cgtctcggcg cggctcagac
      481 gctccccgcg tctcggggcg attgtcgatg gcgacgtcaa gcgtgaggac tgcgcgatcc
      541 gtcgctgagg actgcgcgat gccagaggct ggtgaacctc gtttgccgtc gctccatgtc
      601 ggcagttgca gcgggattcg gtgatcggtc gctgggagtg gcccagaagc gacgacgggc
      661 ggcagtaagc gctgacagcg cccgaccgat gtggctcccg gaagggttgg tcgcagctca
      721 ggcacccgac tggcacgaga agcatctgga gagcggttca gcggttcttg accggagtct
      781 gatgatctcg gcagcgcccc gagacgccag ccgttagcgc ccgccgggcc gcggatcgag
      841 gtgtccaatg cgtcgtcctc agttgtctac cggtgcatgg ctgttctgcg gcatcgtcgc
      901 cgctgccctg atcgcgccgg ccggcgtcta tgccgcggtc aactcgcacg tcgccatcgg
      961 caacgtgggg aacgcgacga cggccactgt cgacgccgag caccagttgc tcacagccgt
     1021 tgtcgcgccg agcgacatcg tgcgcatctc cggggccgca aacgtcgcct cgaacggatg
     1081 ctcggtgatc tacacaccgc cgaccgggaa ggcactcgtt cttgtcaatg tcaccgtcga
     1141 actcgagatc cagcccggcg ggacgtttgt cgcggatgtc atttcgtcga ggaaggactg
     1201 cggcgccccg gtcgattggt tcgagacaac cgtcgacagg catgaggaac agcacaccta
     1261 ccctgccggt ctgccacttg caaggctgtc gctcggtacg ggctatgcgt caggcatctg
     1321 ggctaccggc tacctcatcc cggcgagcca gcttccggcg accatcgctc cgtccggcaa
     1381 gctacctcct ttccatcgct gaggaagggg ggctgcaccc ggtcagctgc aaacacaatg
     1441 cctgacctcg acgcttctcg ggcggaaatc gacgccgcat ggcggccggg cgagtaggac
     1501 gagcaaaggt cgaccgttca tgcgtcactg accgcgacgc catggcggca gcgttccgag
     1561 tcgtcctggg tctgcctcag gtcctgccct gccgccgaag ggaggagcgg tccgcatgac
     1621 ggatacaaag cagcggacgc gggtcgcgac gtacgagcga gtgagcaccg aggaccggcg
     1681 cgagcgcgag accatccgga cgcaaaccga gcagttggag cgtcggctgt cgctcgagga
     1741 ggtggagatg gtcgcccgct acgtcgacga tggcatctcg ggctgccgcc ggcgcttccg
     1801 tgagcgcgaa ggcggcggcc aactgctcca cgacgcggag gcggggcggt tccaagagtt
     1861 gtgggtctat cacaccgacc ggctggcccg gaacctctcg gacatggccg cgaccggcag
     1921 gtgcacgaag agctctgtat caccgtggtg agcgtggttc acgggcggat gaaccctttc
     1981 cttttcgatc tgatcgccgt gatggcacaa aacgagcgac gcaccctcct ccgtcgctcg
     2041 gcagacggca ttgcaagggc catcggcgaa ggccgctacg tgggcggcat cgtgcccttc
     2101 ggatacaggg tggtcggcga gaaggcaaag gcccgtctgg agccccacac cgcgcacttg
     2161 cgcagcgatc agtcggcagc cgacatcgtg atccagatgt accgctgggt cgggctggag
     2221 ggctggagct gccggcgcgt ggcgggcacg ctgaacctcc actcggtgcc aacgcgctac
     2281 gtacgggacg gtcggtcggt caagcgcgga gagcgccgcg agcggacaca aggcatctgg
     2341 cgtgacgggc gtgtgagcaa cctgctcgtc agcccgatct accgcggtga gtcgacgttc
     2401 ggcaagcgga gcgagaagcg cgaccgctca gtcctccgcg gctcggtcga accgctggtc
     2461 tcccaggagc tgtggcaggc agcgcaggag acgctcgccc gcaaccgggt ctgcgccaag
     2521 aacacccgtc gaaccgacct ccggcgcgga aacctccatt gcgcggagtg cgggctcagc
     2581 tacgttgggt c
//
LOCUS       DASSGX010000609         2276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1082684, whole genome shotgun sequence.
ACCESSION   DASSGX010000609 DASSGX010000000
VERSION     DASSGX010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082684"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(490..1758)
                     /locus_tag="VFI12_11055"
     CDS             complement(490..1758)
                     /locus_tag="VFI12_11055"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HET7056993.1"
                     /translation="MVEYRRRVLDNTLDELQAHLRALSIHGPKGVGKTATATQRAVSV
                     VDLSLPAQREIIRADPSILSRLPGPVLVDEWQRFPEVWDQVRRAVDSGSPPGHFIIAG
                     SSAPRGAVIHSGAGRIVPMRMRPLSVAERDVETSTVSLAALLAGNRDVDGSTRLRLPD
                     YVEEITASGFPAIRGLPLRVRRAELDAYLDNVVEREFPEQGYPVRRPAVLRAWLAAYA
                     AATSTTATYSKILDAATAGLANKPAKETTLAYRDALSSLWLLDDLPPWIPGHNHLDRL
                     GQSARHHLADPALAARLLGLDAAALLRAEQGSINLSEGTILGALFEHLVALSVHTYAE
                     AAEGRVSHLRTRNNIHEVDLIVQHADGRVLGIEVKLADRVEDIDTKHLHWLRDRIGED
                     LIDAVVIYAGEHAYRRRDGVAVVPLALLGP"
     gene            complement(2068..>2276)
                     /locus_tag="VFI12_11060"
     CDS             complement(2068..>2276)
                     /locus_tag="VFI12_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056994.1"
                     /translation="TAGCSGVVPGSMWYTGTCPRVVVVPRGFDRGRDGEELMREIKPG
                     YGDRSPRPRVSPLSGRLAEFAKRR"
BASE COUNT          430 a          807 c          692 g          347 t
ORIGIN      
        1 cggacccgac ctcgctatcg cagtccccga tcaatgactg atcgaccaca cccacccacg
       61 gctggaaccg gatcccacca tcgcagacca ccgcccgagc accgccaccc accgcttcca
      121 ggtctccgac gccgccgtcc cgcaacaatc gctcagcggc cacgattacc tgcgccgaga
      181 tcgaactacg aaacgcggaa acatcgatac gaactgccac gagcgctcac gataccgcca
      241 gagccacagc gcttcagagc agggcaaagc accctactcg gccatctctc cgcgccgcga
      301 gccaccctcc gccgacgcca cacctcgcga accgcctgcc ggacaacctg accgccggtt
      361 cgcatgcgag atcgaagtgg gccggacgtt gcaccatgcg aaccacccgg ccactgtccc
      421 cggccatccg ctgtcaggat gtttgaccag ttcaaggaag gcatcgtcgg cttcgtgacc
      481 gagctcagat caggggccga gcaatgccaa agggacgacg gctacaccgt cgcggcggcg
      541 atacgcatgc tcgccagcgt agatgacgac ggcgtcgatc aggtcttcgc cgatccggtc
      601 acgtagccaa tgcaggtgct tggtgtcgat gtcctcgacg cggtcagcca gcttgacctc
      661 gatgcccagc acccttccat cggcgtgctg gactatgaga tccacctcgt ggatgttgtt
      721 acgagtccgc aggtggctta cccggccctc ggccgcctcg gcgtaggtgt ggacgctgag
      781 cgccacgagg tgctcgaaga gagcgccgag gattgtgcct tccgacaggt tgatactgcc
      841 ttgttcggcc cgtagcagcg cagcggcatc gagccccagc agccgagcgg ccagcgccgg
      901 gtcggctagg tggtgcctgg cggattggcc gagccgatca agatggttgt ggccgggaat
      961 ccacggtggc agatcgtcga gcagccacaa cgacgacagt gcgtcgcgat aggcaagtgt
     1021 ggtctccttg gccggcttgt tcgccagccc ggcggtagcc gcgtcgagga tcttcgagta
     1081 ggtcgccgtc gtcgatgtcg cggcggcata cgcggcaagc caagcccgta atacagccgg
     1141 acgacgaacc gggtagccct gctcagggaa ctcccgctct acgacgttgt ccagatacgc
     1201 gtccaactct gcccggcgga cacggagcgg cagcccacgg atggcgggga acccggacgc
     1261 ggtgatctcc tcgacgtagt cgggcagccg gagccttgtg cttccatcga cgtcgcgatt
     1321 gccggccagc agagcggcca ggctgacggt agaagtctcg acatcccgtt ccgccacgct
     1381 gagcgggcgc atccgcatcg ggacgatgcg tccggcaccc gagtggatca ccgcgccccg
     1441 aggcgccgaa ctgccggcga tgatgaagtg cccgggcggg gagcctgagt caaccgcgcg
     1501 acgaacctgg tcccagacct cggggaaccg ctgccactca tccaccagca ccggccctgg
     1561 cagacgagac aagatggagg gatccgcacg aattatctct cgctgggcag gaaggctcag
     1621 atcgacgacc gacacggcgc gctgcgtcgc cgtcgccgtc ttccccacgc ccttggggcc
     1681 gtggatcgat agagcacgca gatgggcttg aagctcatcc agtgtgttgt ccaggacacg
     1741 ccgccggtac tcgaccacaa ggccactcta cccggagcac gccttctacc catccaatcg
     1801 cacagccatt tatcttccag atgcacggag tctagtcttc cattcgcgca ctcccggcgg
     1861 gtcaagtatt acccgagccg gacgatgcac ctcggtcgcc agcccagccc ggccggaccc
     1921 ggctggagat cgacagtacg atccgcgcga cagcggccgc cttcaccgaa ccgacactgc
     1981 gcagcctcga cgccatccac ctcgacagcg ccaaggcagc ggggctgccc acggcgggtc
     2041 cgggtctgga gtagaagcga accccgatca gcgtcgtttc gcgaactctg ccaggcgccc
     2101 gctgagcgga ctcactcgcg ggcgcggcga acggtcgcca tagcccggct tgatctcgcg
     2161 catcagttcc tcgccatccc ggccccgatc gaatccgcgc gggacgacca cgactcttgg
     2221 gcatgtaccc gtgtaccaca tcgaacctgg gacaacgccc gagcaacccg cggttc
//
LOCUS       DASSGX010000610         2217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_869186, whole genome shotgun sequence.
ACCESSION   DASSGX010000610 DASSGX010000000
VERSION     DASSGX010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869186"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..1094
                     /locus_tag="VFI12_11065"
     CDS             270..1094
                     /locus_tag="VFI12_11065"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET7056995.1"
                     /translation="MTPADLQSLPVRPPDHRLAYGDDPNQFGELRLPSGAGPHPVVVL
                     IHGGCWKADYATLRDLGPMGDALESEGIATWNIEYRRLGQPGSGWPGTYLDVGRGIDH
                     LRQVAPRYGLDLARVAVVGHSAGGHLAMWAATRHRLSPKSSVYLANPLPLRGVVNLAG
                     TIDMRENISHMEGECHDAVVTTMLGGTPAKVPERSREVSATTMVPLGVQQLLVWGERE
                     QFVPTVLARRHVDAATKAGDRARLIVVPAVGHFELASPQSSAWRTVLDAVRSLVVN"
     gene            1282..>2217
                     /locus_tag="VFI12_11070"
     CDS             1282..>2217
                     /locus_tag="VFI12_11070"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET7056996.1"
                     /translation="MNEQLSPDRILQTGLAFWPSKTLLSAIELGVFTELARGPESVDV
                     LRSRLGLHPRSALDFLDTLVALGFLSRTGDRYANTPETDLFLDRSKPSYLGGMLEMAN
                     HRLYPFWAHLTEGLRTGLPQNEIKTGDAGLFETLYTDPARLKQFLAAMTGLSHGANMK
                     IARAFPWTNYRTFVDVGTAQGDLAAQIAKANTHLRGVGFDLPEVATIFEEYVALVGVA
                     DRLTFVPGSFFAQDLPKADVVLMGHILHDWDLPAKKMLIQKAYDAVSAGGALVVYESI
                     IDDDRSQNAFGLMMSLNMLIETPGGFDYTGADCAGW"
BASE COUNT          384 a          720 c          706 g          407 t
ORIGIN      
        1 atctgtccaa gatcgcgccg gtcgattgat tgaaacaacc gaataaactt cgtgcctccg
       61 cggctccgtg ttgatcccgt cccgtggcct ccgtcccctc cgtccgctcg gtccgagcgc
      121 cggcgaggct cgcaccaaac acctcgttga gctaccctgt ctgcatgcgt atcccactcg
      181 cgtcgcttct cgtgctcgcg gcagtcgccg gcgtcttgca gccctcgaca cacgcgtcgc
      241 agcgtgggtc ggcgacgaca cggccgctga tgacgccggc ggacctgcag tcgctcccgg
      301 tgcgtcctcc tgaccatcga ctcgcctacg gcgacgatcc caatcaattt ggcgagttgc
      361 gtctgccgtc gggcgccggc ccgcatcccg tcgttgtcct gatccacggc ggatgctgga
      421 aggcggatta cgcgacgctg cgcgatctgg gcccgatggg cgacgcgctc gagtcggagg
      481 gcatcgccac ctggaatatc gagtaccgcc gcctcggcca gccaggaagc ggatggcccg
      541 gcacctatct ggatgtcgga cgcgggatcg accatctccg tcaggtggca cccaggtacg
      601 gcctcgacct ggccagagtg gctgtggtgg gccactccgc cggaggccat ctggcgatgt
      661 gggcggcaac gcgtcaccgc ctgtcaccga agagttccgt ctacctggcg aatccgcttc
      721 cgcttcgcgg cgtcgtcaac ctggccggca ccatcgacat gcgcgagaac atttcgcaca
      781 tggagggcga gtgccacgat gcggtcgtca ccacgatgct gggcggaacg ccggcaaagg
      841 tgcccgagcg gtcccgggag gtctccgcga ccacgatggt tccgttgggg gttcagcagc
      901 tcctcgtgtg gggagagcgc gagcaattcg tgccgacggt gctggcgagg cggcatgtcg
      961 acgcggcgac caaggccggc gaccgcgccc gcctgattgt cgtcccggca gtcggccatt
     1021 tcgaattggc gagcccgcag tcgtcggcgt ggcggacggt cctcgacgct gttcgttccc
     1081 tggtggtcaa ctgagcggcg taaacgtcgg gaactcaagt tgaccgacga tcgccaccag
     1141 tagtcgatcc tcggattacc gctccaccag atcgcaaact cgcacgcgcc cggaatgaat
     1201 caagggatcg ctggaaacag cgaaggtctt ccggtcgggt cgaggcttga cgccggtcgc
     1261 ctttcagagc aggagcattg gatgaacgag caactgagcc ccgacaggat tctccagact
     1321 gggctggcct tctggccatc gaagactttg ttgagtgcca tcgagctggg tgtgttcacc
     1381 gaactggcgc gcgggccgga gagcgtcgat gtccttcgca gtcgactcgg cctgcatccg
     1441 cgatcggccc tcgattttct tgacaccctg gtggcactcg ggttcctgtc gcgcaccgga
     1501 gaccgttacg cgaacacccc tgagaccgat ctcttcctcg atcggagcaa gccgtcctac
     1561 ctgggcggaa tgctggaaat ggcgaaccat cgtctctatc cgttctgggc tcacctgacc
     1621 gaggggctgc gcacggggct cccgcaaaac gaaatcaaga cgggtgatgc cggcctgttc
     1681 gaaacgctgt ataccgatcc agcacggctc aagcaattcc tcgccgccat gaccggcctc
     1741 agccacggcg ccaacatgaa gatcgcgcgg gcgttcccgt ggacgaacta ccgcacgttc
     1801 gtggacgtgg ggacggcgca aggggatctc gcggcgcaaa tcgcaaaagc caacacgcac
     1861 ttgcgaggcg tcgggttcga cttaccggaa gtcgccacga ttttcgagga atatgtcgct
     1921 ctcgttggcg tcgccgaccg cctgacgttc gtgccgggca gcttttttgc gcaagacctg
     1981 cccaaggccg acgtggtgtt gatgggccac atcctccacg actgggatct gcccgcgaag
     2041 aaaatgctca tccagaaggc gtacgacgcg gtttcagccg gcggcgccct cgtggtctac
     2101 gagtccatca tcgacgatga ccgatcgcag aacgccttcg ggttgatgat gagccttaac
     2161 atgctcatcg agacgcctgg ggggttcgac tacacgggcg cggactgcgc gggttgg
//
LOCUS       DASSGX010000611         2083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_321201, whole genome shotgun sequence.
ACCESSION   DASSGX010000611 DASSGX010000000
VERSION     DASSGX010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321201"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="VFI12_11075"
     CDS             complement(<1..926)
                     /locus_tag="VFI12_11075"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET7056997.1"
                     /translation="MKRRSFLTGSAALAASPLLAGGTLRLASAQDLSGKLVLWHGWTG
                     AEADTLNNDILPAWKTANPNVEVQALAVPFDQLKNKYSTDTATGGGPDLLIGPADWVG
                     ELATGELIAPLDEMATSDITDAYLPATVDQLKFDGKLYGMPESFECVALYYNTDLVKE
                     APKDTNEVYTVSAAMPSGTYGLALLSNFYHPAGFLFGFGGQLFDDQKKCILNSPETVN
                     FLTWLGNFTTKPGLFQQNDDGAISSLFKSAKAGMVVNGPWALGDYTGALGEDKVKVAP
                     LPLISEHDNGPAKPFLGVKHIMMNSNIEGDQA"
     gene            complement(1058..1780)
                     /locus_tag="VFI12_11080"
     CDS             complement(1058..1780)
                     /locus_tag="VFI12_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7056998.1"
                     /translation="MAEVQIWIGSEWAAVREALVAHLRQWPAGALAVITTSLDPATPT
                     GRIAGWVRAAGAETGVSVTARQPGDAFDLAPAPDRAVIGITELLLDPDPAQAEQLYAL
                     GLWSRFAPRKRRLLMRVTPFLDEEIVTNAELASPSLLLQIAEWTGQRVLIVATDLIAA
                     EVIGRGLRSIAQPAGDVGLNPWQTELVRIASERRLGVTSGPQIALIVHRAGVMPPPVA
                     AAFRDCVERVARLVDCRVSFEG"
BASE COUNT          319 a          687 c          720 g          357 t
ORIGIN      
        1 ttggcctgat cgccctcgat gttgctgttc atcatgatgt gcttcacgcc gaggaagggc
       61 ttcgccgggc cattgtcgtg ctcggagatc aacggcagcg gcgcgacctt caccttgtcc
      121 tcgccgagcg cgccggtgta gtcgccgagg gcccacggac cgttgacgac catgccggcc
      181 ttcgcgctct tgaacagcga gctgatcgcc ccgtcgtcgt tctgctggaa gaggcccggc
      241 ttggtcgtga agttgccgag ccaggtcagg aagttgacgg tctccggcga gttgaggatg
      301 cacttcttct ggtcatcgaa gagctggccg ccgaagccga agaggaagcc ggccgggtgg
      361 tagaagttgg agagcagcgc caggccgtag gtgccgctgg gcatcgccgc ggacaccgtg
      421 tagacctcgt tcgtgtcctt cggcgcctcc ttgaccaggt cggtgttgta gtagagggcg
      481 acgcactcga agctctccgg catgccgtat agcttgccgt cgaacttgag ctgatcgacg
      541 gtggccggga gataggcgtc cgtaatgtcg ctggtcgcca tctcgtcgag cggggcgatc
      601 agctcgccgg tggccagctc gccgacccag tcggccgggc cgatcagcag atccgggccg
      661 ccgccggtcg cggtatcggt gctgtacttg ttcttgagct ggtcgaacgg caccgccagc
      721 gcctggacct cgacgttcgg gttcgcggtc ttccaggccg gcaggatgtc gttgttgagc
      781 gtgtcggcct cggcgccggt ccagccgtgc cagaggacga gcttcccgct caggtcctgc
      841 gcgcttgcca gccgcagcgt gccgcccgcc aggagcggtg atgcggccag cgccgccgag
      901 cctgtgagaa atgaccgtcg cttcatgctg atcctcctcg atcaaatcag gcgcgccggg
      961 gccggagcga acatcgcccg cgcgagtgcc acggcacgcc attgaaccgt gtactgatga
     1021 ttgtgtacca catggcgcgg cagcgttgcg ggtttggcta accctcgaat gaaacccggc
     1081 agtccaccag ccgggcgacc cgctcgacac agtcgcggaa ggcggcggcg accggcggcg
     1141 gcatgacccc ggcccggtgg acgatgagcg cgatctgtgg cccggacgtg acgccgagcc
     1201 ggcgctcgct ggcgatccgg accagctccg tctgccacgg gttcaggccg acgtcgccgg
     1261 ccggctgcgc gatcgagcgg agcccccgcc cgatcacctc ggcggcgatc aggtcggtcg
     1321 cgacgatcag gacgcgctgt ccggtccact ccgcgatctg gaggagcaga gaggggctgg
     1381 ccagctcggc attggtgacg atctcttcgt cgagaaaggg cgtgacgcgc atcagcagcc
     1441 ggcgtttgcg cggcgcgaac cgggaccaga ggccgagggc atacagctgc tccgcctgtg
     1501 ccgggtcggg gtcgagcagg agttccgtga tgccgatcac ggcgcggtcg ggtgcgggcg
     1561 cgaggtcgaa ggcatcgccc ggttgccgcg ccgtcactga tacgccggtc tccgcacctg
     1621 cggcgcgaac ccagccggcg atccgcccgg tcggcgtggc ggggtcgagc gacgtggtta
     1681 tcaccgcgag cgccccggcc ggccattggc gcaggtgggc caccagcgcc tcccggaccg
     1741 ccgcccattc gctcccaatc cagatctgca cctcagccat gtccatgagc gccatgatac
     1801 cggcccggtt aatcccgggg gttctaccgg cacggggctt gtaggggcgt gcgggggcac
     1861 gcccgcgccc cgcccgatcg cctggcgcca caatccagtc cggccaccat ggcgatgcgg
     1921 ggggaaccag ggcggatttg cccccgcaaa tccctacaac ggcatcgttt gtccgctcgg
     1981 gtaataattc caacatggag aacaaattgc cggatcggca atcgcatcgc ttgcgcgggt
     2041 atgactattc ggctccaggt tcctatttcc tgacgatctg cac
//
LOCUS       DASSGX010000612         2873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1083065, whole genome shotgun sequence.
ACCESSION   DASSGX010000612 DASSGX010000000
VERSION     DASSGX010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083065"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..1233
                     /locus_tag="VFI12_11085"
     CDS             190..1233
                     /locus_tag="VFI12_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HET7056999.1"
                     /translation="MSTAAAPTNRYIRQEILGVVRPEGQEKLAGAHVALIGAGALGST
                     IADLLVRAGIGTLTLIDRDYVELHNLQRQALYTEADVQNHVPKAMAAAERLASINSTI
                     EIRPVVADVNSLSIEQLTAEADFLVDGTDNFETRYLINDLAVKSGRPWIYGGVIATYG
                     MTMTIIPGETACLRCVFPDAPDAGSAPTCDTAGVFGPAVHVVSSLEASEAIKLALGRT
                     DAVNRSLISLDVWSMELQRIPVGGPRPDCPTCQGRQFDFLDRQALNLETTLCGHDAVQ
                     VMIQPPVQLDLAVLAKRLEPAGEVMANRFLVRFTDAVTGHELTIFPDGRAIVKGTTEP
                     REARAIYDRYVGS"
     gene            1237..2391
                     /locus_tag="VFI12_11090"
     CDS             1237..2391
                     /locus_tag="VFI12_11090"
                     /inference="COORDINATES: protein motif:HMM:TIGR01977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET7057000.1"
                     /translation="MIYLDNAATSWPKPEPVRQAVIEALDRFGANPGRSSHQMAIQTA
                     EMIYRTRAALARLINGQAPERIAFCTNATDALNLAIFGLVRPGDRVVTSSMEHNSVRR
                     PLRMMQDAGAEVITVEASKTGVVDPDLVRAVADNSKLIAITHGSNVNGAVQPIAEIGR
                     IAREHGALLLVDAAQTIGAMPIDVQAMQIDLLAFPGHKSLLGPSGTGGLYIGERVDVG
                     QLRPLHAGGTGLRSEDDIQPLELPYRYESGTVNTLGIAGLSAGVKYVQTMGIEAVQAH
                     HAERRATLATGLAEIGVTMFEPNQAEAADVVSFEIPGWQPADVGSVLDQAFGIACRTG
                     LHCAPGACATIGAGPNGTIRFSPGLTTTDEEISAAIEAVAAIASEAMVRR"
     gene            2388..2804
                     /locus_tag="VFI12_11095"
     CDS             2388..2804
                     /locus_tag="VFI12_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7057001.1"
                     /translation="MSDPNVNPKRSRLEEEVLEILQRTDRTPPVRARARAWGRVVRAR
                     ILDARSHVGWLDTAWGWFGLALLLFILGGWVAGDSGLLKQVLQYAGLAAVIIGLFRLW
                     RPGSGSQRKMWRGRQIDLRKPGVELGDKFDDWRKRR"
BASE COUNT          482 a          860 c         1079 g          452 t
ORIGIN      
        1 tggtaacccc gcgctacggt tccgggttcc gatgactcac caagtcctcc gggagccggg
       61 cgggccgccc ggggctacat ctaccatttc ggctctcccg ccggtgatgg cgcaagccgg
      121 gagaagggcg gttgaacagg cgtgctatag aattgatagc atcggatttg acatcgcgcg
      181 ggagagcgga tgagcacggc ggctgcgccc accaatcgat atatccggca ggagattctc
      241 ggcgtcgtcc ggcccgaggg gcaggagaag ctggccgggg cgcatgtcgc cctgatcggg
      301 gccggggcgc tcggcagcac gattgcagac ctgctggtgc gggcggggat cgggacgctg
      361 acgctgatcg accgcgacta cgtcgagctg cataacctgc agcggcaggc gctctatacc
      421 gaggccgacg tccagaacca tgtcccgaag gcgatggcgg cggcggagcg gctggcatcg
      481 atcaattcga ccatcgagat acgtccggtt gtggccgatg tcaacagcct ctcgatcgag
      541 caattgaccg ctgaggccga cttcctggtc gacggcaccg acaacttcga gacgcgctac
      601 ctgatcaacg acctggccgt gaagtcgggc aggccgtgga tctacggcgg ggtaattgcg
      661 acctatggca tgacaatgac catcatcccg ggcgagacgg cctgcctgcg ctgcgtcttc
      721 ccggacgcgc cggatgccgg gtcggcgccg acctgcgata cggccggcgt gttcggcccg
      781 gcggtgcatg tcgtgtcgtc gctggaggcg agcgaggcga tcaagctggc gctcggccgg
      841 accgacgcgg tcaaccggtc gctgatctcg ctcgatgtct ggtcgatgga gctgcagcgg
      901 atcccggtcg gcggtccgcg gcccgattgc ccgacctgcc aggggcggca gttcgacttc
      961 ctcgaccggc aagcgctgaa tctcgagacg acgctctgcg ggcacgacgc cgtgcaagtg
     1021 atgatccagc cgccggtgca gctcgatctg gcggtgctcg cgaagcggct tgagccggcc
     1081 ggcgaagtga tggcgaaccg gttcctggtc cgattcactg acgcagtgac cgggcacgag
     1141 ctcacgatct tcccggacgg tcgcgcgatc gtgaagggga cgaccgaacc gcgcgaggcc
     1201 cgggcgattt acgaccgata tgtggggtcc tgagccgtga tttacctcga caatgccgcg
     1261 acgagttggc caaagccgga gccggtccgc caggccgtga tcgaggcgct cgaccggttc
     1321 ggcgcgaacc cgggccgctc cagccaccag atggcgatcc agacggccga gatgatctat
     1381 cgcacccgcg cggcgctggc ccggctgatc aacggccagg cgccggagcg gattgcgttc
     1441 tgtacgaacg cgaccgacgc gctcaacctg gcgattttcg ggctcgtgcg gccgggcgac
     1501 cgggtcgtga ccagctcgat ggagcacaac tcggtccggc ggccgctgcg gatgatgcag
     1561 gacgcgggcg ctgaggtgat caccgtcgag gcgagcaaga ccggcgtcgt cgatcccgat
     1621 ttggtcaggg cggtggcgga caactcgaag ctgatcgcga tcacgcacgg gtcgaatgtg
     1681 aacggggcgg tgcagccgat cgccgagatc gggcggatcg cgcgggagca tggggcgctg
     1741 ctgctggtcg atgcggcgca gacgatcggg gcgatgccga tcgacgtgca ggcgatgcag
     1801 atcgacctgc tggcgttccc ggggcacaaa tcgttgctcg ggccgagcgg gacgggcggg
     1861 ctgtacatcg gcgagcgggt cgatgtcggc caattgcggc cgctgcacgc cggagggacc
     1921 gggctgcgct ccgaggatga cattcagccg ctcgaattgc cttaccggta cgaatcgggg
     1981 accgtcaaca cgctgggtat cgcagggctg tcggcgggcg taaagtatgt ccagacgatg
     2041 ggtatcgaag cggtccaggc gcaccacgcc gagcggcggg cgacgctggc gacgggtctg
     2101 gccgagatcg gcgtgacgat gttcgagccg aatcaggcgg aggcggccga cgtcgtatcg
     2161 ttcgagatac ccggatggca accggccgat gtcggatcgg tgctggatca ggcgttcggg
     2221 atcgcctgcc ggaccgggct gcactgcgcg cccggggcct gcgcgacgat cggggcgggg
     2281 ccgaacggca cgatccgctt cagcccgggc ctgaccacga ccgacgagga gatcagcgcc
     2341 gcaatcgagg cggtcgccgc gattgcgagc gaggcgatgg tgcggagatg agcgatccga
     2401 acgtgaaccc gaagcgctcc cggctcgagg aggaggtgct cgaaatcctg cagcgaaccg
     2461 accgcacgcc accggtgagg gccagggcgc gcgcgtgggg ccgagttgtc cgggcacgga
     2521 tccttgatgc gcggtcgcat gtcggctggc tcgatacggc ctggggctgg ttcgggctgg
     2581 cgctgctgtt gttcattctc ggtggctggg ttgcgggcga ttctggattg ctgaagcagg
     2641 tgctgcaata cgcggggctc gcggcggtaa tcatcgggct gttcaggctc tggcggccgg
     2701 gttccggatc gcagcggaag atgtggcgcg ggcggcagat cgatctgcgc aagccgggtg
     2761 tcgagcttgg ggacaagttc gatgattggc ggaagcgtcg gtaggcgagc aggcgcgaag
     2821 gcgcgaaggc gcgaaggcgc gaaggcgcga aggcgcgaaa ttatagcgtc gca
//
LOCUS       DASSGX010000613         2421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_758415, whole genome shotgun sequence.
ACCESSION   DASSGX010000613 DASSGX010000000
VERSION     DASSGX010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_758415"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VFI12_11100"
     CDS             <1..210
                     /locus_tag="VFI12_11100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET7057002.1"
                     /translation="GEPKLILADEPTGNLDTAHGEEVMRMLRQLNQEGSTIVMVTHSP
                     AHADFASRVVNMLDGRILQERRRAA"
     gene            239..>2421
                     /locus_tag="VFI12_11105"
     CDS             239..>2421
                     /locus_tag="VFI12_11105"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7057003.1"
                     /translation="MWRNYWTVAVRALAKNRTYSVINIAGLAIGMAACILILLYVRYE
                     QSYDQWLPDAENTYQFQAWYPHPQDGDPLFSQMSAYVTKDRIKKDFPQVERVGYILGF
                     APTILKDGQATAADDYGLADDDFLQVVKLPMLSGTTLRSAGTAVLSQSEAIKRFGTDQ
                     VVGQTLTAVSHGEATDYKITGVYKDIPKHSHLKLNAILRTDYDHYWADTPDFRTQWGW
                     QSGWVYANLRPGTDLARIQAAMPAWEKRNIPDESSDGITTNQGDDQDWHFVNVRDVHL
                     GKAQNGAMTPGNDQRTVTTFAVIALLILGMAVVNFTNLATARASQRAREVALRKVLGA
                     NRRQLVVQFVSEAVFISAAAMLIALALVELLVRPFAAFLDADLALNYLGSGGILLPAI
                     ALTLVVGIVSGLYPAIFISRFQPAQVLKANRSAAETPGSGRLRAALVILQFAVSIGLI
                     ICTVVVYAQTVYARTADPGFKRDHLLQVEYVGRREILPRVEGLVEQMKRVPGVLAVGL
                     TDLGVSTDNNITNSVIPAGTTKQVDLGNYEVDQGFFDAMGLKLIAGRWFDPNRPTDDQ
                     TLSFPRDPEAEKAMTERGVNVVINALAAKRLGYGSPESAIGKVFRSRLYSDDATNLTV
                     IGVVGDSRFRTVRTPIQPLFFRKSRSGEGWMEIRYRGNPQAVRSAVEAKWKQVASNVP
                     FQAKFSEDIVGELYKKEDARAKVFAAFSLLAVIIGCLGLFGLAAFT"
BASE COUNT          431 a          812 c          767 g          411 t
ORIGIN      
        1 ggcgagccca agctgatcct cgccgacgag ccgaccggca acctcgacac cgcgcacggc
       61 gaggaagtga tgcggatgct tcgtcagctc aatcaggagg gatcgacgat tgtcatggtg
      121 acccactcgc ccgctcacgc cgatttcgcg agccgggtcg tcaacatgct cgacggccgc
      181 atcctgcagg agcgccgccg cgcggcgtga ccgacggcct ttcgcgagga gcggagccat
      241 gtggaggaac tattggacgg tggcggttcg ggcgctcgcc aagaaccgga cctattcggt
      301 gatcaacatc gccgggctcg cgatcggcat ggccgcgtgc atcctgatct tgctctatgt
      361 ccgctacgag caaagctacg accagtggtt gcccgacgcc gagaacacct atcaattcca
      421 ggcctggtac ccgcacccgc aggacggcga ccctctgttc tcgcagatgt cggcctacgt
      481 caccaaggac cggatcaaga aggatttccc gcaggtcgag cgggtcggct acattctcgg
      541 gttcgcgccg acgatcctga aggacggcca ggccaccgcc gccgacgact acggcctcgc
      601 cgatgacgat ttcctgcagg tcgtgaagct gccgatgctg tcgggaacga ccctgcggtc
      661 ggccggcacc gcggtcttgt cgcagtcgga ggcgatcaag cgcttcggca ccgaccaggt
      721 cgtggggcaa acgctgaccg ccgtctccca cggcgaagcg accgattaca agatcacggg
      781 cgtctacaag gacatcccca aacactcgca tctcaagctc aacgccatcc ttcgcaccga
      841 ctacgatcat tattgggccg acacgcccga tttccgcacg caatggggct ggcagtccgg
      901 ctgggtgtac gcgaacctgc gcccgggcac cgatctcgcg cggatccagg ccgcgatgcc
      961 ggcatgggag aaacgcaaca ttcccgacga gtccagcgac ggcatcacca ccaatcaggg
     1021 cgatgaccag gactggcact tcgtcaacgt ccgcgacgtc catctcggca aggcgcagaa
     1081 tggggcgatg acccccggca acgaccagcg gacggtcacc acgttcgcgg tgatcgcgtt
     1141 gctgatcctc ggcatggctg tcgtcaattt caccaacctt gcgaccgcac gggcgagtca
     1201 gcgggcgcgc gaggttgcgc tgcgcaaggt gctgggcgcc aaccgccggc agctggtcgt
     1261 ccagttcgtc agcgaggcgg tgtttatttc ggccgcggcg atgctgatcg cgcttgctct
     1321 ggtcgagctt ctcgtccggc cgttcgccgc cttcctcgat gccgacctcg cgctaaacta
     1381 cctcggcagc ggcggaatcc tgttgccggc catcgcgctc acgctggtgg tcgggatcgt
     1441 cagtggcctc tatccggcga tcttcatcag tcggttccag ccggcgcagg tcctcaaagc
     1501 gaaccgctcg gcggcggaaa cccccgggtc cggccgcctg cgcgccgcgc tcgtgattct
     1561 ccagttcgcg gtttcgatcg gcctgatcat ctgcaccgtg gtggtgtacg cccagacggt
     1621 ttacgcccgc accgccgacc ccgggttcaa gcgcgatcac ctattgcagg tcgagtatgt
     1681 cggccggcgc gaaatcctgc cgcgcgtcga ggggctggtc gagcagatga agcgggttcc
     1741 aggcgtactc gccgtcggcc tcaccgacct cggcgtctcg accgacaaca acatcaccaa
     1801 cagcgtcatc ccggccggaa cgaccaagca ggtcgacctc ggcaattacg aagtcgacca
     1861 gggatttttc gatgcgatgg gcctgaagct gatcgccggc cgctggttcg atccgaaccg
     1921 gccgaccgac gaccagacgt tgtcgttccc gcgcgatccc gaggccgaaa aggcgatgac
     1981 cgagcgcggc gtcaacgtgg tcatcaatgc gctggcggcg aagcggctcg gctacggatc
     2041 gccggagagc gcgatcggga aagtgttccg gtcgcggctc tacagcgacg atgcgaccaa
     2101 cctcactgtg atcggcgtcg tcggcgattc acgcttccgc accgtgcgca cgccgatcca
     2161 gccgctgttc ttccgcaaga gccggagcgg cgagggctgg atggagatcc gctaccgcgg
     2221 caacccgcag gcggtgcgct cggccgtcga ggccaagtgg aagcaggtcg cgagcaacgt
     2281 ccccttccag gcgaagttca gcgaggacat cgtcggcgag ctctacaaga aggaagacgc
     2341 gcgggcgaaa gtgttcgccg ctttttcgct gctcgcagtg atcatcggct gccttggcct
     2401 gttcggcctt gccgccttca c
//
LOCUS       DASSGX010000614         2019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_869699, whole genome shotgun sequence.
ACCESSION   DASSGX010000614 DASSGX010000000
VERSION     DASSGX010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869699"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VFI12_11110"
     CDS             complement(<1..430)
                     /locus_tag="VFI12_11110"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET7057004.1"
                     /translation="MPEPIAFPLRGGPTVRGLAWSGGPDTVILIHDIGGDLDSWGALP
                     ATLAAEGYRVVAIDLPGHGLSDDPWDSERAPEQVAEIVSATRTDPGRCFGIAIGSLAP
                     VIGDLVVDTLVVVSPHAPAVPERPGAASPCLIFVGGADPGS"
     gene            complement(438..950)
                     /locus_tag="VFI12_11115"
     CDS             complement(438..950)
                     /locus_tag="VFI12_11115"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET7057005.1"
                     /translation="MTDEQAAQTDESWRGKVGKLSQAEMEEFLATDVLCRLAVLDKDG
                     WPYIVPAWFQYKDGGFYIVPRERSAWAELMKADPRVYLSIDEPGRQRKVMVKGEAQLL
                     EEPNLGGKWVEVAIEMSYRYLGPNGPSYLEPTLTEPRWLFFVKPIKITTWQGVDWAKK
                     YKHSGWGAND"
     gene            complement(1058..1480)
                     /locus_tag="VFI12_11120"
     CDS             complement(1058..1480)
                     /locus_tag="VFI12_11120"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET7057006.1"
                     /translation="MKPRITVLTLGVDHLERAVAFYRDGLGLPTDGIVGTEFEHGAVA
                     FFDLQAGLKLAVWPRDDLAHDAGIPRNAPSPTEFSIGHNVLLRDEVDAVMEQARAAGA
                     TIVKAAQDTFWGGYSGYFQDPDGHLWEIVWNPDLLPPD"
     gene            complement(1532..>2019)
                     /locus_tag="VFI12_11125"
     CDS             complement(1532..>2019)
                     /locus_tag="VFI12_11125"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7057007.1"
                     /translation="FTWSFPLILIAVVLAGALGPGLKATVLAIGLINWAGFARVIRGE
                     VLALREQEYIQAAKAIGVSDARILLRHVLPNIVAPTLVMASYYVALAIIAEAGLSFIG
                     MGAQPPLPSLGAMVAGGRNFLQFDHWISTIPGVAIVLIVLGLNFLGDGLRDLLDPRLR
                     R"
BASE COUNT          351 a          710 c          639 g          319 t
ORIGIN      
        1 cggatcccgg atcggccccg ccgacaaaga tcaggcacgg cgatgccgcg cccggccgct
       61 ccgggaccgc cggcgcgtgc ggggagacca cgaccagggt atctacgacc agatcgccga
      121 tgaccggggc gaggctgccg atggcgatcc cgaagcaccg gccgggatcg gtccgcgtgg
      181 ctgaaacgat ttcagcaacc tgctccggcg cgcgctccga atcccacggg tcgtccgaga
      241 ggccgtgccc gggcaggtcg atcgcaacga cgcgataccc ctcagcggcg agcgtggccg
      301 gcagggctcc ccaggaatcg aggtcgccgc cgatgtcgtg gatcaggatg accgtgtccg
      361 gcccgcccga ccaggcgagg ccgcgcaccg tcgggccgcc gcgcagcggg aaggcgattg
      421 gttcaggcaa cggcgcgcta atcgttggcg ccccacccgg aatgcttgta cttcttcgcc
      481 cagtcgacgc cctgccaggt cgtgatcttg atcggcttga cgaagaagag ccagcgcggc
      541 tcggtcaatg tcggctcgag gtagctcgga ccgttcggcc cgaggtagcg gtaggacatc
      601 tcgatcgcaa cctcgaccca cttgccgccg aggttcggct cctcgaggag ttgcgcctca
      661 cccttgacca tcaccttgcg ctgccggccc ggctcatcga tcgagaggta gacgcgcgga
      721 tcggccttca tcagctcggc ccaggctgag cgctcgcgcg ggacgatgta gaagccaccg
      781 tccttgtact ggaaccaggc cggtacgatg tagggccagc cgtccttgtc cagcaccgcc
      841 agccggcaga ggacatccgt cgccaggaac tcctccattt ccgcctgcga gagcttccca
      901 accttccccc gccacgattc gtctgtctgc gccgcctgct cgtctgtcat cagatctcct
      961 tccatccatt gcgccattct tgcaaacatt ccgaagctgc ggtgcggccc ggatgctcac
     1021 acctgcgctg gtaccaggac gagcgcggac gccggaatca gtccgggggg agcaaatccg
     1081 ggttccagac gatttcccag aggtggccgt cgggatcctg gaagtaaccg gagtagcctc
     1141 cccagaacgt gtcctgcgcg gccttgacga tcgttgctcc cgccgcccgg gcctgttcca
     1201 ttacagcgtc cacctcgtct ctcaggagca cgttgtggcc gatcgagaac tcagtcgggg
     1261 acggcgcatt cctgggaatc ccggcgtcat gggcgaggtc gtcgcgcggc cagacggcca
     1321 gcttcagacc cgcctgcaaa tcaaagaacg caacggcgcc atgttcgaac tccgtgccga
     1381 cgattccgtc ggtcggcagg ccgagaccgt cacggtagaa cgcgaccgcc cgctccaggt
     1441 gatcgacgcc gagcgtcagg actgtgattc ggggcttcac actatcctcc agacgtgcgg
     1501 gcattcgccc tctgtgctca atccgtcctc gtcaccgccg caacctcgga tccaacaggt
     1561 cgcggaggcc gtcgccgagg aagttcaggc cgagcacgat caggacgatc gcgactccgg
     1621 ggatcgtgct gatccagtga tcaaactgga ggaagttccg gccgccggcg accatcgcgc
     1681 ccaggctcgg caagggcggc tgcgccccca ttccgatgaa ggagaggccg gcctcggcga
     1741 tgatcgccag cgccacgtag tacgacgcca tcacgagcgt cggcgcgacg atgttcggca
     1801 gcacgtgccg cagcaggatc cgggcgtcgc tgacaccgat cgccttcgcc gcctggatgt
     1861 actcctgctc ccgcagcgca agcacctcgc cacggatcac ccgcgcgaaa ccggcccagt
     1921 tgatcagccc gatcgcgagc accgttgcct tcaggcccgg tccgagcgcg cccgccagca
     1981 cgaccgcgat caggatcagc gggaagctcc aggtgaact
//
LOCUS       DASSGX010000615         3210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_649975, whole genome shotgun sequence.
ACCESSION   DASSGX010000615 DASSGX010000000
VERSION     DASSGX010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649975"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="VFI12_11130"
     CDS             <1..875
                     /locus_tag="VFI12_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7057008.1"
                     /translation="TGLAQDATPATSNLFAALGLPVLTITATDAGLSVDQAEIAAGRY
                     LVTLNDETGNPDLATGFVRLDEGETLADLGSSDEIAAGTPALSDEEQTPETYQFLFDN
                     YITGGPSQLSPQVVVNLPAGEYGIWPDDPFAETPVAPLTVTGDPTAEITGSEPNAAVT
                     IVEEGEGGQGFKFTVDGQFTAGPQIVKIINASDQPHIVAPLQYPEPITQDQLANWMMF
                     DPSTGETPPADLLDDSKITSPGYAAIQSAGSTQWVTMDLAAGQVALLCFIPDPVTGGT
                     PHFMEGMMQVFDVG"
     gene            complement(1296..1586)
                     /locus_tag="VFI12_11135"
     CDS             complement(1296..1586)
                     /locus_tag="VFI12_11135"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HET7057009.1"
                     /translation="MMNASEPPEAEIASSPSAVAVEENSQPVVHSDPEIMGRTPVFYG
                     TRVPVDAMFDYLAGNYCLDKFLDGFPTVSPAQALDALEIGREAVLRASARPD"
     gene            complement(1615..>3210)
                     /gene="lon"
                     /locus_tag="VFI12_11140"
     CDS             complement(1615..>3210)
                     /gene="lon"
                     /locus_tag="VFI12_11140"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HET7057010.1"
                     /translation="IKKTKLSKEAREKAMAEVKKLRTMGPMSAEATVVRNYLDWMLSI
                     PWKKRSKIRNDVVEAEKVLEADHYGLEKVKERIIEYIAVRKLAGDRLRAPIICFVGPP
                     GVGKTSLGRSIARAIGRNYVRMSLGGVRDEAEIRGHRRTYVGAMPGRVIKALKDAKSR
                     NPVLVLDEVDKVGSDAFRGDPSSALLEVLDPEQNTTFSDHYLEVPFDLSKVIFITTAN
                     LLEPIPPALRDRMEIIEVSGYTEVEKMAIGRQFLLPKALASHGLEAEQLTVTDDALRR
                     LIREYTEESGVRNLEREIGSLCRKVARTLAGDDPPATIAVDAPQVSDYLGVPKYDYGL
                     AEEQDEIGVATGAAVTSVGGDLLSIEVLIMPGKGDLILTGQLGDVMQESARAALSYAR
                     ARTSTYGLAPDFFDRHNIHVHVPAGAIPKDGPSAGITMATALISALTGRQVRKDVAMT
                     GEITLRGKVLPIGGLREKTVAAHRGGIKTFILPRRNAKDLAELPAVVRDGMELIQVDT
                     LDEVLAAAILPEQQSGPVSVQVA"
BASE COUNT          539 a         1061 c         1044 g          566 t
ORIGIN      
        1 ctacggggct ggctcaggac gccacgcccg cgacgtccaa tctcttcgcc gctctcggct
       61 tgccggtact gacgatcacg gccaccgacg cagggctgtc cgtcgatcag gccgaaatcg
      121 cggccggccg ctatctggtc acgctgaacg acgaaacggg caatcctgac ctggcgaccg
      181 gattcgtgcg gctcgacgag ggggagacgc tggcggatct cggctcctcc gacgaaattg
      241 cggctggaac gccggcgctg agtgacgagg agcagacacc ggaaacctat cagttcctct
      301 tcgacaacta catcaccggc ggtccctcac aactctcgcc gcaggtcgtg gtcaatttgc
      361 ccgcgggcga atacggcatc tggccggatg acccctttgc cgagacgccc gtcgcgccgc
      421 tgaccgtgac cggcgacccg acggcggaga tcacagggtc cgagccgaac gctgcagtga
      481 cgatcgtcga ggaaggcgaa ggcggccaag gcttcaagtt caccgtcgac gggcaattca
      541 ctgcgggccc gcagattgtc aagatcatca acgccagcga tcagccccac atcgtggccc
      601 cgctgcaata tccggaaccg atcacgcagg atcagctcgc gaactggatg atgttcgacc
      661 catcgactgg cgaaacgcca ccagcggatt tgctcgacga ctcgaagatc acctccccgg
      721 gttatgctgc tatccagtcg gcggggagca cgcagtgggt gacgatggat ctggctgccg
      781 gccaggtggc gctgctctgc ttcatccccg atcccgtgac cggcggcacg ccgcacttca
      841 tggaggggat gatgcaggtg tttgacgtcg gctgacgcag ccggtcagcc gggtaatgag
      901 gaagccggca agtcgcttcc tgactcgccg gcttcctgcg gtcgtatgct ccagcacgga
      961 ttaagatcgg cgtgagcgac caccacgcgg gtcgacgccg acgtggagca caccgccggg
     1021 tccgatgctc tcgagtgtgc tcgatctgtg gagccaacgg gagaacgtca ttcgggttcg
     1081 tcctgggccc catcaccacg atcacgcaga gcccgaagcg ggcaacgtcc tgttggacat
     1141 ccaagtcgcg gtcggcagta atcagcacgc cgaaacctgc cgcttcggag cggcgcagca
     1201 atgcaccatt gctcgtgccg cgccacccca tctgtgtcac cgtgcgggcg tcatgggcag
     1261 tcagggcatc actgggtcgc cgagagagcg actcatcagt caggacgagc actcgccctc
     1321 aacaccgcct cacggccaat ctccagagca tccagggctt gcgccgggct gacggtgggg
     1381 aaaccatcga ggaacttgtc caagcagtag ttgcctgcga ggtagtcgaa catcgcgtcg
     1441 acgggcaccc gcgtcccgta gaagaccggc gtcctgccca tgatctccgg atcgctgtga
     1501 acgaccggct gggaattctc ctcgacagcg accgctgatg gtgaggaggc gatctccgct
     1561 tctggcggct ccgaggcgtt catcatcgtt ctgttccatt cccgtccagc cggtctacgc
     1621 cacttgcacc gagacaggcc cgctctgctg ctcgggcaga atggcggcgg ccaggacttc
     1681 gtccagcgta tcgacctgga tcagctccat gccatcgcgg acgaccgcgg gtagctcagc
     1741 cagatccttg gcgttgcggc gcggcaatat gaaggtcttg atcccgccgc ggtgagcggc
     1801 gaccgtcttt tcgcggagcc cgccgatggg gagcaccttg ccgcgcaggg tgatctcccc
     1861 ggtcatcgcc acgtccttgc gcacctggcg gcccgtcagg gcggagatca gcgcggtcgc
     1921 catggtgatg ccggcggagg ggccgtcttt cgggatggcg ccggccggga cgtgaacgtg
     1981 gatgttgtgc cggtcgaaaa agtcgggcgc caggccgtag gtgctggtgc gcgcccgcgc
     2041 gtacgagagg gcggcccgcg ccgattcctg catcacatcg ccgagctgcc cggtgaggat
     2101 gagatccccc ttgccgggca tgatcagcac ctcgatgctg aggagatcac cgccgacgct
     2161 ggtgacagcg gcgccggtcg ccacgccgat ctcgtcctgc tcctcggcca gcccgtagtc
     2221 gtactttggc acgccgagat agtcgctgac ttgcggcgcg tcgacggcga tcgtggccgg
     2281 gggatcatcg cccgccagcg tgcgggccac tttgcggcag agagaaccga tctcccgctc
     2341 cagattgcgg acccccgact cctcggtgta ctcgcggatc aggcggcgca gggcgtcgtc
     2401 ggtgacggtc aattgctccg cctcgaggcc gtgcgaggcg agcgccttcg gcagcaggaa
     2461 ctgccggccg atcgccatct tctcgacctc ggtgtacccc gagacctcga tgatctccat
     2521 ccggtcgcgc agcgccggcg ggatcggttc gagcaggttg gcagtggtga tgaagatcac
     2581 tttcgagaga tcgaacggca cctcgagata gtggtcggag aaggtcgtgt tctgctccgg
     2641 atcgagcacc tcgagcagcg ccgatgaggg atcgccccgg aaggcgtccg agcccacctt
     2701 gtcgacctcg tccagcacca ggaccgggtt gcggctcttg gcgtctttca gcgccttgat
     2761 gacgcgtccg ggcatggcgc cgacgtaggt gcggcggtga ccgcggattt cggcctcgtc
     2821 gcgcacgccg ccgagcgaca tgcggacgta gttgcggccg atggcgcggg cgatcgagcg
     2881 gcccaggctc gtcttgccga cacccggcgg gccgacgaag cagatgatcg gcgctcgcag
     2941 gcggtcaccc gccagcttgc ggacagcgat gtactcgatg atccgctcct tgaccttctc
     3001 cagcccgtag tggtcggctt ccaggacctt ctccgcctcg accacgtcgt tgcggatctt
     3061 gctgcgcttc ttccacggaa tcgacagcat ccagtcgaga tagttgcgca cgaccgtcgc
     3121 ctcggccgac atcgggccca tggtgcggag cttcttgacc tccgccatgg ccttctcgcg
     3181 cgcttccttg gagagcttcg tcttcttgat
//
LOCUS       DASSGX010000616         2946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_869863, whole genome shotgun sequence.
ACCESSION   DASSGX010000616 DASSGX010000000
VERSION     DASSGX010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869863"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1833)
                     /gene="infB"
                     /locus_tag="VFI12_11145"
     CDS             complement(<1..1833)
                     /gene="infB"
                     /locus_tag="VFI12_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HET7057011.1"
                     /translation="MAQGFNSGGPRGGRRSGGKRNTRGRSGGARTVTLRRTAPAVREP
                     ITLPPVLTVAELADKTETTPIEVIKELMRIGVMANINQQIDYDTAHRVATALGWESSE
                     DVPEIVQRANADFESRMLEAMSDPSATGRPPVVTIMGHVDHGKTKLLDSIRQARVAEG
                     EVGGITQHIGAYQVETQGRRVTFLDTPGHEAFTAMRARGAQVTDVAVLVVAADDGVMP
                     TTREAVAHIKSASVPMIVAINKIDLPTANPDRVKQQLAEIEVVPEEWGGDVPFVEVSA
                     REKLGLDDLLDVILLVSDVHELKANPNRLANGTVIEAKVDRNRGPVATVLIQSGTLNM
                     RDIVVAGATWGRVKAMLDDHGRRVRKAGPSFPVEILGLLEVPQAGDQVQVFDDEKEAR
                     ELVESRVQQRRVEQLTADRAMKLTDIASQVNAGGSQTAELRVILKTDVQGSLGAIQAA
                     LLKLNDETDAGVRLSITYAGTGAISESDVSLAVATKSVIVGFNVRPDVAAKRAADSSG
                     VDIRFYNIIYNLIDEVKQAMTGLLAPVFQDVTDGYAEVRETFKLPNGDVVAGCYVLDG
                     KLVRNSRVRVLRSGTVMFEGGIKSLRRFKDDVREVAAGYECGLGL"
     gene            complement(1843..2223)
                     /locus_tag="VFI12_11150"
     CDS             complement(1843..2223)
                     /locus_tag="VFI12_11150"
                     /inference="COORDINATES: protein motif:HMM:NF016204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HET7057012.1"
                     /translation="MSKPENQAMMEQTSSAPGPKGKSRKGPRPKHVPIRTCAICREQG
                     TKRELIRVVRQPDRSVAIDPSGRLNGRGAYICDKASCWDRAASTDVLAKALNVDLSQE
                     FRDALRQFGRAAANDAVEPGPTGK"
     gene            complement(2216..>2946)
                     /locus_tag="VFI12_11155"
     CDS             complement(2216..>2946)
                     /locus_tag="VFI12_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HET7057013.1"
                     /translation="QLIVSRSHPNLIRRLFEIEVPEIYSGAVEIMAIAREPGLRSKVA
                     VAARQEKVDPVGSCVGVRGVRIQNIVNELYGEKIDVIEWSPDTATFIANALSPAKPTN
                     VSLSEADNIATVIVPSDQMSLAIGKEGQNARLAYKLTNWRIDIKDPESLKDGDLEMLR
                     QARSDYQQETAGSLAWQGRQPRLVRPDGMVAVREQEYGPLPDDLIGMSVDVDIAGDAL
                     EVYYNRALRARFDVASGDQLPLDE"
BASE COUNT          478 a          987 c          915 g          566 t
ORIGIN      
        1 gagccccagg ccgcattcgt atcccgcggc aacctcgcga acgtcgtcct tgaagcgccg
       61 gagggacttg atgccgccct cgaacatgac cgtaccactc cgcagcacac gaacccgcga
      121 attgcgcacc agcttgccgt cgagcacgta gcagccggcg acaacatcgc cgttcggcag
      181 cttgaaggtc tcacggactt cggcgtaccc gtcggtcacg tcctggaaga ccggtgcgag
      241 cagaccggtc atcgcctgct tcacctcgtc gatcaggttg tagatgatgt tgtagaaacg
      301 aatatcgacg ccgctgctat ccgcagcccg cttggccgcg acgtccggcc ggacgttgaa
      361 gccgacgatg acgctcttcg tcgccaccgc gaggctcacg tctgactccg aaatcgcgcc
      421 cgtgccggca taggtgatgc ttagccgcac gcccgcatcg gtctcatcat tcagcttgag
      481 caatgccgcc tggatcgcgc ccagcgaccc ctgcacatcc gtcttcagaa tcacgcgcag
      541 ttcggcagtc tggctgccgc ccgcgttgac ctgcgaggca atgtcggtca gcttcatggc
      601 gcgatcggcc gtcagctgct cgacccggcg ctgctgaacc cgggactcca cgagctcccg
      661 cgcctccttc tcatcgtcga agacctgaac ctgatcgccg gcctgcggga cctccaaaag
      721 gccgagaatc tcgaccggga agctcggtcc ggccttgcga acgcggcggc cgtgatcgtc
      781 gagcatcgcc ttgacgcggc cccaggtggc gccggccacg acgatatcgc gcatattgag
      841 cgtaccgctc tggatcagca ccgtcgcgac cggaccgcga ttgcggtcca ccttcgcctc
      901 gatgacggtg ccgttggcca gccggttcgg gttggccttc aactcgtgca cgtcagagac
      961 gagcaagatc acgtcgagca ggtcgtccag gcccagcttt tcgcgggccg agacttcgac
     1021 gaaggggacg tcgccgcccc actcctcggg cacgacttcg atctcggcga gctgttgctt
     1081 gacgcggtcc ggattcgccg tcggcaggtc gatcttgttg atcgccacga tcatcggcac
     1141 gctggccgac ttgatgtggg caactgcctc gcgcgtggtc ggcatgacgc cgtcatcagc
     1201 cgccaccacg agcacggcca catccgtcac ctgggcgccg cgcgcccgca tggcggtgaa
     1261 tgcctcgtga ccaggggtgt cgaggaaggt cacgcggcga ccctgcgtct caacctggta
     1321 ggcgccgatg tgctgggtga tgccgccgac ctcgccctcg gcgacgcgtg cctggcgaat
     1381 cgagtcgagc agcttcgtct tgccgtggtc gacgtggccc atgatggtca caaccggcgg
     1441 gcgacctgtc gcgctcgggt cgctcatggc ctcgagcatg cggctttcga agtccgcgtt
     1501 cgctcgctgg acgatttcgg gtacgtcctc gctgctttcc cagccgaggg cagtcgcaac
     1561 ccggtgagca gtgtcgtagt cgatttgctg gttgatgttg gccatcacac cgattcgcat
     1621 cagctccttg atgacttcga tcggcgtggt ttcggtcttg tccgccaatt cggcgacggt
     1681 caaaaccggg ggtagcgtga ttggctcacg cacggccggc gctgtgcggc ggagcgttac
     1741 cgtgcgtgcg ccacccgagc gaccgcgcgt gttccgcttg ccgcccgagc gtcgacctcc
     1801 gcgcggtccg cccgaattaa atccttgtgc catatgggta tctcactttc cggttgggcc
     1861 gggctcgaca gcatcatttg ccgccgcccg gccaaattgg cgaagcgcgt cgcgaaattc
     1921 ctgcgacaaa tccacgttga gcgccttcgc cagaacgtca gtggaagctg cccggtccca
     1981 gcagctcgcc ttgtcacaaa tgtatgcgcc gcgaccgttg aggcggccgc tcgggtcaat
     2041 tgccaccgac ctgtcgggtt gacgcacgac gcggattaat tcgcgtttcg tgccctgctc
     2101 ccggcagatg gcgcatgtcc tgatcgggac atgcttcggg cgcggtccct tgcgcgattt
     2161 ccccttcgga cccggcgcgc tggatgtctg ctccatcatc gcctggttct ccggtttact
     2221 catccagtgg caactggtcg ccactggcca cgtcaaatcg ggcgcgcagc gcccggttgt
     2281 aatagacctc aagcgcatcg ccggcaatgt cgacgtcaac gctcatgccg atcagatcat
     2341 cgggcagcgg tccgtattcc tgctcacgga cggcaaccat gccgtctgga cggaccaggc
     2401 gcggctgccg tccctgccag gcaaggctgc cagccgtctc ctgctggtaa tccgagcggg
     2461 cctggcgcag catctcaagg tccccatcct tgagcgactc cggatccttg atgtcgatcc
     2521 gccagttggt cagcttgtag gcgaggcgag cgttctgccc ttccttgccg atcgccagcg
     2581 acatctggtc ggatgggacg atgaccgtgg cgatattgtc ggcctcggag agtgagacgt
     2641 tggtcggctt ggccgggcta agcgcgttgg caatgaaggt cgccgtgtcg ggcgaccact
     2701 cgatgacatc gatcttctcg ccatacaact cgttgacgat gttctggatg cgcacgcccc
     2761 ggacaccgac gcaggaaccg acgggatcga ccttttcctg gcgggcggcg accgccacct
     2821 tggagcggag accgggctcc cgggcgatgg ccatgatctc aaccgcgccg ctgtagatct
     2881 cgggaacctc gatctcgaac aaacggcgga tcaggttcgg gtgcgagcgc gacacgataa
     2941 gctgcg
//
LOCUS       DASSGX010000617         6518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_214501, whole genome shotgun sequence.
ACCESSION   DASSGX010000617 DASSGX010000000
VERSION     DASSGX010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6518
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214501"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..922
                     /gene="rnc"
                     /locus_tag="VFI12_11160"
     CDS             80..922
                     /gene="rnc"
                     /locus_tag="VFI12_11160"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HET7057014.1"
                     /translation="MSRRSKAHSIQPVDDQTDNAARLAEAIGVAFHDLDLLRLALTHR
                     SVAHELRAQGPHMELPLSLRSNERLEFLGDAVLGFLVADDLYRRFPDAPEGDLTSRRV
                     ALVRAERLVSWARSINLGDYVYLATGERISDSERDRILSGGFEALIGAITLDQGIAAT
                     AAFMQPFLDADAAGSLEEWVAANPKGQLQEYVQEHYRQPPIYRIIDAEGPDHARIFTA
                     EVLIDETVIGSGSGESKRLAEQAAAAEALDQLRQPRAPKAGRPTVRKRRKPRAPKGGT
                     ELDG"
     gene            915..1904
                     /locus_tag="VFI12_11165"
     CDS             915..1904
                     /locus_tag="VFI12_11165"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HET7057015.1"
                     /translation="MGRVYDELTIFGGNSTPELTDAVCAYLGLPRGRAEVFKFSNDNT
                     FVRIGESVRENDVFVIQSFAEPVNDAIMELLIMIDTLRRASAGRITAVIPYFGYGRTD
                     KKDQPRVPITASLVAKLVGVSGADRVLAIDLHAGQIQGFFEIPVDEMSAMFLTANYFV
                     EKRLTNPVVVSPDLGNTKRARNFAQILDAPLAIIEKRRIGNEDRSQILNLIGSVADAQ
                     VVIVDDEIDTAGSITQAAQVCIQEGATEVYATCVHPVLSGPAIERLQESPIREIVATD
                     TIRVPPAKRIDKLTILSIAPLLGETIQRIHTGTSVGAAYRAAGGAMTLRMQLY"
     gene            2051..3121
                     /locus_tag="VFI12_11170"
     CDS             2051..3121
                     /locus_tag="VFI12_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7057016.1"
                     /translation="MPLTTTRRSVIAAALLSGLVTRRTGAQTLGKKGSDSPLALLPAL
                     PGDPSTILTWADIAGYCKATGIEKPASFDDATTETWIKVGQRIPLNDNLVFLNMNQPW
                     QDLIGFAPWDIDQVGIAFDPPAQLRIYQGAFDPERVARQVTTNKGFTSASAEGFTILD
                     NPSDDLDLTSDFDRISLGNLNHLAVSDSLVLASRSKSVRDAAIAVAGGDQDAVARMPE
                     FADVAEAIGGEHGFILLNGSILGPGYAGRDAGSLPGVIPPARLFFGSIRVDGDDWRTA
                     FAADLGDPVAAADAIAVVEERIATQSSVVARTPYTELLGDPDVESVAGTGIVRVLVTE
                     EHFDRRWMNVIFAGDLLFLATE"
     gene            complement(3317..5122)
                     /locus_tag="VFI12_11175"
     CDS             complement(3317..5122)
                     /locus_tag="VFI12_11175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET7057017.1"
                     /translation="MAHRASPSYFQRVHRRAQHRTATTAPGRHSAARQPNVGQRRYAQ
                     LRHAPAQAGVTATLPETTPSTLIRTTPIAALGMELAQLKKAQDKRHGPKWWSGIAARP
                     IRSAIIAIVILSLTGILAIAGPIALRSYQAYQDVFVTQVPDQPGGFTANLNEQGTPVI
                     VANSTDVAAQIPEWDGTSRLTLLLLGVDRREGDYARSDTIILVNVDPVEKTARMLSIP
                     RDLKVIIPGFGAHKINAAYAFGDANANVPGGGPGLMMRTIETNFGINIDYFAEVDFNG
                     FVKIVNTVGGVTLDVPYPIRDNEYPAPNNQYMRIYFPSGWQHMNGERVLQYARTRHDD
                     GDGRRSARQQQVLLSLRQQALTLGLIKQAPTLISEVGDTVRTDLSADQALKLARLATE
                     IDQAHIQQMTLDPALITWQTDEFYFQGDWAMIGDIMTEFTGQKIVPPMSALANPRYDT
                     AISILDGTLIPGLGPRVLDVVKSNGFEDATTVDLPDAGNYPATQIIVSPDNIATAYLL
                     AGILGVPETAITASDEIQTPTIVATNTPAQTAVAATPAGTPTAGDGKLDVVPLFPTVA
                     PAPGSASAQPAGEIVIILGDDLPDPAWYDVDPNGQ"
     gene            5303..5890
                     /gene="dcd"
                     /locus_tag="VFI12_11180"
     CDS             5303..5890
                     /gene="dcd"
                     /locus_tag="VFI12_11180"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HET7057018.1"
                     /translation="MILSDVDIRRALAEGRIRITPEPDLDAQLGSVSVDFRLGTTFMV
                     FEHSRHSFIDPRSPQSIGDAMRTIEVAPDEPFIMQPGDFALASTVESLELPDNLLGRL
                     EGRSSIARLGITVHSTAAVFEPGWIGTATMELSNLGRMAVALYPGMRICAFSFEEVSS
                     PVSVPYRLKRNNKYAGQLTPRASQLSDEASLAVER"
     gene            5974..6360
                     /locus_tag="VFI12_11185"
     CDS             5974..6360
                     /locus_tag="VFI12_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7057019.1"
                     /translation="MPVDQLSSIRDRFLPMLAYGQNEYGYRVPAGYPIIHDDVERGVI
                     GFELDPMHSIHVTTDGEQIYADVFYRSQRYDVRSSASREKFAGRGVVDRRWLGPHPTD
                     QQLRNLIAELMSRFNQQQTMIYFTDD"
BASE COUNT         1091 a         2051 c         2196 g         1180 t
ORIGIN      
        1 acaccggcca tcgcgcggcg ctccgtataa tcgcgacgtg ccggcgactg ccgggatgtc
       61 aatcgcccgg gaacccgcca tgagccgtcg atccaaagca cattcgattc agcccgtcga
      121 cgaccagaca gataacgcgg cgcgtctggc ggaggcgatc ggggtcgcgt ttcacgatct
      181 cgacctgttg cggttggccc tcacccaccg gtccgtcgcg cacgagctgc gggcgcaggg
      241 gccgcatatg gaactgccgc tctcgctgcg gtcgaacgag cggctcgagt tcctcggcga
      301 cgccgtgctc ggtttccttg tcgcggacga tctctatcgc cgatttccgg acgcgcccga
      361 gggcgacttg acatctcggc gggtggccct ggtgcgggcc gagcggctgg tgtcgtgggc
      421 tcggtcgatc aatctcggtg attatgtcta tctggcgacc ggcgagcgga tctccgacag
      481 cgagcgcgac cggatcctct ccggcgggtt cgaggcgctg atcggagcaa tcacgctgga
      541 tcagggcatt gcggccacgg cggccttcat gcagccgttc ctcgatgccg acgcggccgg
      601 ctccctcgag gagtgggttg cggccaatcc gaagggccaa cttcaggaat acgtgcagga
      661 acactaccgg cagccgccga tctaccgcat tattgacgct gagggcccgg accacgcccg
      721 catctttacc gccgaggtcc tgatcgacga aacggtcatc ggcagtggaa gtggcgagag
      781 caagcggctg gccgagcagg cggccgcggc agaggccctc gatcagctca ggcaaccccg
      841 ggcgccgaag gctgggcgtc cgaccgtacg caaacgacgg aagccgcgcg ctccgaaagg
      901 tggaacagag ctcgatgggt agggtctacg acgaattgac catctttggc ggcaactcga
      961 cccccgagtt gaccgacgcg gtctgcgcct acctcggatt gccgcgcggg cgggcagagg
     1021 tcttcaaatt cagcaacgac aacaccttcg tccggatcgg cgagagcgtc cgtgagaacg
     1081 acgtcttcgt gatccagtcc ttcgcggagc cggtcaacga cgcgatcatg gagctgctga
     1141 tcatgatcga taccctccgc cgcgcatcgg ccggccggat cacggccgtc atcccctact
     1201 tcgggtacgg ccggacggac aagaaggacc aaccgcgggt gccgatcacg gccagcctcg
     1261 tcgcgaagct ggtcggcgtc tcgggagccg atcgcgtgct ggcaatcgac ctccacgccg
     1321 gccagatcca gggcttcttc gagatcccgg tcgacgagat gagcgcgatg ttcctgaccg
     1381 cgaactactt cgtcgagaag cggctgacca acccggtcgt ggtctcgccg gacctcggca
     1441 ataccaagcg cgcccgcaac ttcgcgcaaa tcctcgatgc gccgctggcg atcatcgaga
     1501 agcggcggat cggcaacgag gatcgctcgc aaatcctgaa cctgatcggc tcggtggccg
     1561 acgcccaggt cgtgatcgtc gacgacgaga tcgacaccgc cggctcgatt acccaggcgg
     1621 cgcaggtatg catacaggag ggcgcgacag aggtctacgc gacctgcgtc cacccggtcc
     1681 tttccggccc ggcgatcgag cggctgcagg aaagcccgat ccgcgaaatc gtcgcgacgg
     1741 acacgatccg cgtgccgcct gcgaagcgga tcgacaagct caccatcctc tcaatcgcgc
     1801 ccctgctcgg cgagacgatc cagcgcatcc acaccggcac cagcgtcggc gcggcctacc
     1861 gggccgccgg cggcgccatg acgctccgta tgcagctcta ttagtgcacc actggacccg
     1921 ggaacttcga tcagcccgtt gacgttgtat acggcgacgg gctggtcggc accggggcgc
     1981 gctgtgatgt gtccccgaca gccacgatgg cctgttttcg ctcgcagtct ggaccaggga
     2041 gtttattgct ttgccactga caacgacgcg ccgctcggta atcgctgccg ctcttctgag
     2101 tgggcttgtc acccgccgaa caggcgccca gacgctcggc aaaaagggct ccgactcgcc
     2161 gttggccctc ctccccgcac tccccggcga tccgtcgacg atcctcacgt gggcggacat
     2221 cgcgggttac tgcaaggcga caggaatcga gaaaccggct tcctttgacg atgcgacgac
     2281 ggaaacctgg atcaaggtag gccagcgaat cccgctcaac gacaacctcg ttttcctgaa
     2341 catgaaccag ccgtggcaag acctcatcgg cttcgcaccc tgggatatcg atcaggttgg
     2401 tatcgccttc gacccgccgg cacaactgcg gatctaccag ggcgccttcg acccggagcg
     2461 cgtcgcccgg caagtcacga cgaacaaggg cttcacctcg gccagtgcag agggcttcac
     2521 gatcctcgac aacccatctg acgacctgga cctgacaagc gactttgacc ggatctcgct
     2581 gggcaacctg aaccacctgg ccgtttcgga ttcgctcgtg ctggcctcgc gctcgaaatc
     2641 ggtccgcgac gccgcaatcg cggtcgcagg tggcgaccag gatgcagtag cccgcatgcc
     2701 ggagtttgcc gatgtggccg aggccatcgg cggcgagcat ggcttcattt tgctcaacgg
     2761 ctcgatcctg ggtcctgggt atgctgggcg cgatgccggg agcctgcccg gcgtcatccc
     2821 accggcgcgc ctgttcttcg gtagtatccg ggtcgatgga gacgattggc gcaccgcatt
     2881 cgccgccgac ctgggcgatc ccgtggctgc ggctgacgcc atcgcggtcg tcgaagagcg
     2941 gatcgcgacc cagtcctccg tggtcgccag gaccccctac accgagcttc tcggagatcc
     3001 cgacgtcgag tccgtggccg ggaccggcat agtcagggtt ctggtgaccg aggagcattt
     3061 cgacaggcgc tggatgaacg tcatcttcgc cggtgacctc ctcttcctcg ccaccgaata
     3121 gggctgtcgg atttcgcggc ggtaggtgcc ggaaatggag cgcggagttg ccacgccccg
     3181 cgtcgttcct cccccgggtg cgttttatga aaccgcaccg gaggcgagat cggttccttc
     3241 caacgtcgtc cccggcgccg gatccgggcg gcacggtaac cccgcgctcc ggtgccgcga
     3301 ttgcagggac tcggcgctac tgcccgttgg ggtcgacgtc gtaccaggcg ggatcgggca
     3361 ggtcatcgcc gaggatgatc acgatctcgc cggccggctg ggcgctggcg gagccgggcg
     3421 ccggggcgac cgtcgggaag agtggcacca cgtcgagctt gccgtcaccg gccgtcggcg
     3481 tcccggccgg tgtcgccgcg accgccgttt gggctggggt gttggtcgcg acgatggtcg
     3541 gtgtctggat ctcgtcgctg gccgtgatcg ccgtctcagg cacgccgagg atgcccgcca
     3601 gcaggtaggc ggtggcgatg ttatctggtg aaacgatgat ctgtgtcgcc gggtagttgc
     3661 cggcgtcggg caggtcgacc gtcgtcgcgt cttcgaagcc gttgctcttg accacatcga
     3721 ggacacgggg cccgaggccg ggtatgagcg tcccatcgag gatcgaaatg gcggtgtcgt
     3781 agcgcgggtt agcgagcgcc gacatcggcg ggacgatctt ctggccggtg aactcggtca
     3841 tgatgtcgcc gatcatcgcc cagtcgccct ggaagtagaa ctcgtcggtt tgccaggtga
     3901 tcagggccgg gtcgagcgtc atctgctgga tgtgggcctg gtcgatctca gtcgcgagcc
     3961 gggccagctt cagggcctgg tccgcgctca gatcggtccg gacggtatcc cccacttccg
     4021 agatcagcgt cggcgcctgc ttgatcaggc cgagtgtgag ggcctgctgg cggagcgaaa
     4081 ggagcacctg ttgctggcga gcggaacggc gaccgtcacc gtcgtcgtgg cgggtgcggg
     4141 cgtattgcag cacccgttcg ccgttcatat gctgccagcc cgaggggaag tagatgcgca
     4201 tgtactggtt gttcggggcc gggtactcgt tgtcccggat cgggtacggg acatcgagcg
     4261 tgaccccgcc gaccgtattg acgatcttga cgaagccgtt gaaatcgact tcggcgaagt
     4321 aatcgatgtt gatcccgaag ttcgtctcga tggtgcgcat catgaggccg ggcccgccgc
     4381 cgggcacgtt cgcgttcgca tcgccgaagg cgtaggcggc gttgatcttg tgcgcgccga
     4441 agccggggat gatcaccttg agatcgcgcg ggatcgagag catccgggcc gttttctcga
     4501 cgggatcgac attgacgagg atgatcgtgt cggagcgggc gtagtcgcct tcgcgccggt
     4561 cgactccgag caggagcagg gtcaaacggc tcgtgccgtc ccattccggg atttgtgcgg
     4621 cgacatcggt cgaattggcg acgatgacgg gtgtgccctg ctcgttcaga ttggccgtga
     4681 agccgccggg ctgatccggc acttgcgtga cgaagacgtc ctggtaggcc tggtacgagc
     4741 ggagggcgat cggccccgca atcgcgagga tgccggtcag ggagaggata acgatggcga
     4801 tgatcgcgga gcgaatcggg cgcgccgcaa tgccggacca ccacttgggc ccgtgtcgct
     4861 tgtcctgcgc ctttttgagc tgcgcgagct ccatgccgag cgccgcgatc ggcgtggtcc
     4921 tgatcagggt cgacggcgtg gtctcgggca gtgttgccgt tacgccggcc tgtgccggcg
     4981 cgtgccggag ctgggcatag cggcgttggc cgacattcgg ctgccgtgcc gcggagtggc
     5041 ggccaggagc agtggtggcc gtgcggtgct gcgcgcggcg atgaacacgc tggaaatagg
     5101 acggggacgc gcggtgggcc actaatcagt ccaatggggc tgggcgggca tctggaagat
     5161 gtgtcggtgc agagtctacc atccgggctt gcgcattccg agccgattgt gccaacgagt
     5221 cgcgggctgc gccgcggcgg tgcggctggc acaattgggg cgggtgcatg tttttcgact
     5281 gtgggcgaag gggcgtttcc tgatgatctt gtcggatgtg gatatacgac gggcgttggc
     5341 cgaggggcgg atcaggatca ccccggaacc ggatctcgac gcgcagctgg ggtcggtgtc
     5401 cgtcgatttc cggctgggca cgaccttcat ggtcttcgag cacagccggc acagcttcat
     5461 cgatccgcgt agcccgcaat cgatcggcga cgcgatgcgg acgatcgagg tcgcgccgga
     5521 cgaacccttc atcatgcagc cgggcgattt cgcgcttgcc tccacggtcg aatcgctgga
     5581 gctgcctgat aacctgctgg gccggctcga gggacgctcc agcatcgccc gactcggaat
     5641 cacggtccat tcgacggcgg ccgttttcga gccggggtgg atcggcacgg cgacgatgga
     5701 gttgagcaac ctcggccgga tggcggtcgc gctctatccg gggatgcgga tttgcgcctt
     5761 ctcgttcgaa gaggtcagct cgccggtgtc ggtcccgtac cggctgaagc ggaacaacaa
     5821 gtacgcgggg cagctcacgc cgcgggccag ccagttgtcg gatgaggcgt cgctggcggt
     5881 ggagcggtag tttcacgtga aacatgctcg acatttgccg ggcaactttc ggataatctc
     5941 agggtcttgc agtgaagtcg gtcaggagca aacgtgccgg ttgatcaatt gagttccatc
     6001 cgcgaccggt tcttgccgat gctcgcctac ggtcagaatg agtatggcta ccgggtgccg
     6061 gccggctacc cgatcatcca tgacgatgtc gagcgcgggg tgattggatt cgagctggat
     6121 ccgatgcaca gcattcatgt cacgaccgac ggggagcaga tctacgccga cgtcttctac
     6181 cgctcgcagc gctacgacgt ccgctcctcg gccagccgcg agaagtttgc cggtcgcggc
     6241 gtggttgacc gccgctggct tggtccgcat ccgaccgatc agcagttgcg caacctgatt
     6301 gccgagctga tgtcgcgctt caaccagcag cagaccatga tctacttcac ggacgattag
     6361 cctcgttcgt accgtcgcaa acatgaaaag cgccgggcgg atcatccgcc cggcgcttat
     6421 gtttcacgtg aaacgtccct actcgaggaa ccagcggtcg atctcccgat cgtacgagca
     6481 gatcgcgccg tcgccgaaga agattccgat ctcccgcc
//
LOCUS       DASSGX010000618         5190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1414322, whole genome shotgun sequence.
ACCESSION   DASSGX010000618 DASSGX010000000
VERSION     DASSGX010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5190
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414322"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VFI12_11190"
     CDS             complement(<1..618)
                     /locus_tag="VFI12_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7057020.1"
                     /translation="MKQIRTVQVGLGLIGGTVVEQIVENRARWRDEGIDIGVAAIVTT
                     GGALASDGDGFTDEQLRAIIVRRHEGGSVADVGKELGVMRIASADFRLDQLGIARPVV
                     MDAAAGSETAPVAEAALGAGGAVVYSNKAPLSQPLTQARSLWDAAASGRVRYETTCGA
                     GLPVISTIRSLLATGDQVIEIVGCLSGTLGAIFSDIAAGTPFSQAI"
     gene            complement(647..2191)
                     /locus_tag="VFI12_11195"
     CDS             complement(647..2191)
                     /locus_tag="VFI12_11195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7057021.1"
                     /translation="MSDTQELTLDDDIDQFDLTCPACGNDLLADATYNDWRVCGRCHR
                     HVWVSARERATMIARAGQLIELPYSEPQVDPVEHHHRLSAADRQDDARQRSALADAVV
                     TGRVSFDGGSVIAALIDAILLPGGLGIVTTDKVIAAIRTAIRERLPMVIVCGGGSQSG
                     LSGLLQGAQSLRLSGAIAELHRAGLPLIAVLTHPAGGNLLGGVAVNADLRLAEPGFDG
                     GETLVPDEIVDRPELIGRIATILNYLRRRGGPDVVMAGSSDAASARLATFDDQPAVVI
                     ALQSNEWKSAQHWSIVRRAQRTASNLNLPIVFAITGSAPLSLDAQVEIRDLLLRHRKP
                     VIGIIQGDLTSAHINLLAVDTVIAASDLAIPGSKTKRYTAQEGRAAGMVDVVAGSELA
                     VPVARAIAESLRLSPARRIARRIHQADCRGAEVPETRELTRLELRDLREVQANVMRSV
                     EEWRHRLEQRDFNLPAFSNFQGLPAFRNMPRPKLQVTKPDLIEMRDKWMNRRRPGQPD
                     RDDEQQ"
     gene            2445..4835
                     /locus_tag="VFI12_11200"
     CDS             2445..4835
                     /locus_tag="VFI12_11200"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET7057022.1"
                     /translation="MSMATEMDTQAPEMHAADSHDLIRVHGARENNLKDISVEIPKRR
                     LTVFTGVSGSGKSSLVFDTIAAESQRMINETYSAFVQGFMPTLARPDVDVLDGLTTAI
                     IVDQERMGTDPRSTVGTATDANAMLRILFSRLGQPQIGPPIAFSFNVPTRTASGAMTV
                     DKGKGERIVVRNLVYQGGMCPRCEGRGSVTDFDLSQMYDDSKSLNEGALTIPGYSMDG
                     WYGRIFRGCGFFDPDKPIREFTKKELDALLYKEATKIKVDGINLTYAGLIPTIQKSFL
                     SKDVDAMQPHVRAFVERVVTFTTCPDCGGTRLNEGARSSKIKGINIADACAMQISDLA
                     AWVRGLDEPSVGPLLTALGESLDSFVEIGLGYLSLDRPAGTLSGGEAQRTKMIRQLGS
                     SLTDVTYVFDEPTTGLHPHDIQRMNDLLLRLRDKGNTVLVVEHEPETIVIADHIVDLG
                     PGAGTAGGEVVYEGTVEELQTSGTITGRHLSDRTGLKETVRKPKDTLEIRGASTHNLQ
                     NVNADIPLGVLVVVTGVAGSGKSSLIHGSVSVGDGVVAIDQTAIRGSRRSNPATYSGL
                     LDPIRKAFAKANGVKPALFSANSEGACPGCNGAGVIYTDLGMMAGVTTICEECGGKRF
                     QASVLEYHFGGKDISEVLAMPVAEAAEFFGTGEARTPAAHAILERLADVGLGYLSLGQ
                     PLTTLSGGERQRLKLATHMAEKGGIYVLDEPTTGLHLADVEQLLGLLDRLVDSGKTVI
                     VIEHHQAVMAHADWIIDLGPGAGHDGGRIVFEGTPAELVAARSTLTGQHLAAYVGA"
     gene            4874..>5190
                     /locus_tag="VFI12_11205"
     CDS             4874..>5190
                     /locus_tag="VFI12_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7057023.1"
                     /translation="MLTRMGKQRQTVGIIGAGQLARMLVEAATPLDIDIALLAAADTD
                     GAARIWPNVTIGSPDDAEAVAAFAATCDVVTFDHELVPEPVLARLEAAGVSLAPSADT
                     MRIS"
BASE COUNT          918 a         1709 c         1742 g          821 t
ORIGIN      
        1 gatcgcctgc gagaagggcg tgccggcggc gatatccgag aagatcgcgc cgagcgtacc
       61 cgagaggcag ccgacgatct cgatcacctg atcaccggtc gcgaggagcg atctgatcgt
      121 cgagatgacc ggcaatccgg cgccgcaggt agtctcgtac cgcacacggc ccgatgccgc
      181 cgcatcccac aacgagcggg cctgagtcag gggctgcgag aggggcgcct tgttcgaata
      241 gacgaccgcg ccgccggccc cgagcgccgc ctcggccacc ggggcggtct cggaccccgc
      301 tgcggcgtcc atcacgaccg gtcgggcgat accgagctgg tcgaggcgga aatcagcgct
      361 ggcgatgcgc atcacgccga gctccttgcc gacgtccgct acgctcccgc cctcatgtcg
      421 tcggacgatg atcgcgcgca gttgctcgtc ggtgaagccg tcgccatcgc tcgccaatgc
      481 gccgccggtc gtgacgatgg ccgccacgcc gatgtcgatc ccctcgtccc gccagcgcgc
      541 gcggttctcg acgatctgct cgacgaccgt gccgccgata agcccgagcc ccacttgtac
      601 cgttcgaatc tgcttcaccc ggcgctccat caaagatgac gtctgatcac tgctgctcgt
      661 cgtcgcggtc gggctggccc ggccggcggc ggttcatcca cttgtcgcgc atctcgatca
      721 aatcaggttt cgtgacctgc aatttcgggc gcggcatatt gcggaaggcc ggcaggccct
      781 ggaagttgct gaatgccggc agattgaaat cgcgctgctc gagccggtgc cgccactcct
      841 cgactgagcg catcacgttc gcctgcacct cgcgcaggtc ccgcagctcg aggcgggtca
      901 gctcgcgcgt ctcgggcacc tcggcaccgc ggcaatcggc ctgatgaatc cggcgagcaa
      961 tgcgccgggc gggactgagc cggagcgact ccgcgatcgc ccgggccacc ggcacggcca
     1021 gttcggagcc ggcgacgaca tcgaccatcc cggccgcgcg accctcctgg gcggtgtagc
     1081 gcttggtctt gctgcccggg atcgcaaggt cggacgccgc gatcacggtg tcgacagcga
     1141 gcaggttgat gtgggcactg gtcaggtcgc cctggatgat gccgatgacc ggtttccgat
     1201 ggcgcaggag cagatcgcga atctcgacct gggcgtcgag cgaaagcggc gcagaaccgg
     1261 tgatcgcaaa gacgatcggg agattgaggt tcgaagcagt ccgctgcgcc cggcggacga
     1321 tgctccagtg ctgcgcgctc ttccactcgt tcgactggag cgcgatcacc acagccggct
     1381 gatcatcgaa cgtggcgagg cgagccgacg cagcatcgct cgaaccggcc atgacgacat
     1441 ccggcccgcc ccggcgacgc aggtaattga ggatcgtcgc gatgcggccg atcagctccg
     1501 ggcgatcgac gatctcatca gggacgagcg tctcgccccc gtcgaagcca ggctcggcca
     1561 ggcggagatc ggcattgacc gcgaccccgc cgagcaggtt gccgccggcc gggtgggtca
     1621 ggacggcaat cagcggcagg ccggcccggt gcaactcggc gatcgcgccc gagagccgca
     1681 gcgactgcgc gccctggagc agacccgaga gaccgctttg cgaacctcca ccgcagacga
     1741 tcaccatcgg caaccgctcg cggatcgcgg tccggatggc ggcaatgacc ttgtcggtcg
     1801 tgacgatgcc gagaccgccc ggcaacagga tcgcgtcgat cagggcggcg atcactgagc
     1861 cgccgtcgaa ggagacccgt cccgtcacga ccgcgtcggc aagggcggac cgctggcggg
     1921 cgtcgtcctg tcgatcggcg gcggaaaggc ggtggtgatg ctcaaccggg tcgacctgcg
     1981 gctcggaata gggcagctcg atcaattggc cggcccgcgc gatcatcgtc gcgcgctcgc
     2041 gcgcggacac ccagacgtgg cggtggcagc gcccgcaaac ccgccaatcg ttgtaggtcg
     2101 catcggcgag caggtcgttc ccacacgcgg ggcacgtcaa atcaaactgg tcgatgtcat
     2161 cgtcgagggt aagttcctgc gtatcgctca tgccttgtcc cggccgatgg tcgattgatt
     2221 gttccattct aggcgagcag gcgaggaggc gagcaggcga gcagttggaa gggacatcgt
     2281 tcgggctcgc tgctccgggc gaatccggcg atcaggtaga ctttggagca ttcccgctac
     2341 cgcattgacc gctgagtatc cgacgtttcg cctggtgccg cggcattcca tgcggggcga
     2401 ccttgacgga gaccgatggc gattccatgc aacagggaga gttgatgagc atggcaacgg
     2461 agatggatac gcaggcgcct gagatgcatg ctgccgatag tcatgatttg attcgggtgc
     2521 atggcgcgcg cgagaacaac ctcaaggaca tcagcgtcga gatccccaag cggcggctga
     2581 cagtgtttac cggtgtttcc ggctcgggca aaagttcgct ggtgttcgac accatcgccg
     2641 cggaatcgca gcggatgatc aacgagacct acagcgcctt cgtgcagggc ttcatgccga
     2701 cgctggcccg ccctgacgtc gatgtgctcg acgggttgac aacggcaatc atcgtcgacc
     2761 aggagcgaat ggggaccgat ccgcgctcca cggtcggcac cgcaaccgat gccaacgcca
     2821 tgctccgcat cctcttcagc cgactcgggc agccgcagat cgggccgcca atcgccttct
     2881 cgttcaacgt cccgacacgg acggcgagcg gcgcaatgac cgtcgacaaa ggcaaggggg
     2941 agcgaatcgt cgtccgcaac ctcgtctacc agggcggcat gtgccctcgc tgcgaggggc
     3001 ggggctcggt caccgacttc gatctgtcgc agatgtacga cgacagcaag tcgctcaacg
     3061 agggcgcact cacgatcccc ggctatagca tggacggctg gtacggccgg atcttccggg
     3121 gctgcggctt cttcgacccc gacaagccga tccgcgagtt cacgaagaag gagcttgatg
     3181 cgctgctcta caaggaagca acgaagatca aggtcgacgg catcaacctg acctacgccg
     3241 gcctgatccc gacgatccag aagtcgttcc tatcgaagga tgtcgacgcg atgcagccgc
     3301 acgtccgggc cttcgtcgag cgtgtggtca ccttcaccac ctgcccggat tgcggtggca
     3361 cgagactcaa cgagggagcc cggtcgtcga agatcaaggg gatcaacatt gccgatgcct
     3421 gtgcgatgca gatcagcgac ctggctgcgt gggtgcgcgg tctggacgag ccgtcggtcg
     3481 ggccgctgct gacggcattg ggcgagagcc tcgattcctt cgtcgagatc ggactcggtt
     3541 acctgtcgct cgaccggccg gccggcaccc tctcgggcgg tgaggcgcag cgcaccaaga
     3601 tgatccgaca gctcggctct tcgctaaccg acgtcaccta tgtcttcgac gagccgacta
     3661 ccggcctcca cccacatgac atccagcgca tgaacgacct gctactgcgt ctgcgggaca
     3721 agggcaacac agtgctcgtc gtcgagcacg agccggaaac aatcgtgatc gcggaccaca
     3781 tcgtcgatct cggtccgggg gcgggcacgg ccggtggcga ggtggtctac gagggcacgg
     3841 tcgaggagct gcagaccagc ggaacgatca ccggccgcca cctcagcgac cggacagggc
     3901 tcaaggagac ggtgcgcaag cccaaggaca cgttggagat ccgcggcgca tcaacgcaca
     3961 acctgcagaa tgtcaatgcc gatatccccc ttggcgtgct ggtcgtggtg accggcgtag
     4021 caggttcagg caagagctcg ttgatccatg gctcggtgtc ggtcggggac ggggtggtgg
     4081 cgatcgatca gacggcgatc cggggctcgc ggcgcagcaa tccggctacc tacagcggcc
     4141 tgctcgatcc gatccgcaag gcattcgcca aggccaacgg ggtgaagccg gccctgttca
     4201 gcgccaactc cgaaggcgcc tgccccggct gcaacggcgc cggggtgatc tacaccgatt
     4261 tggggatgat ggccggcgtc acgaccatct gcgaggaatg cgggggcaag cgattccagg
     4321 cgtccgtgct ggagtaccac ttcggtggca aggacataag cgaggtgctg gcgatgccgg
     4381 tggccgaggc cgcggagttc ttcggcaccg gcgaggcacg cacgccggcc gcgcacgcca
     4441 tcctcgaacg gctcgccgac gtcgggcttg gctacctgtc gctggggcag ccgctgacga
     4501 cgctctccgg cggcgagcgg caacggctca aactggcgac ccacatggcc gagaagggcg
     4561 ggatctacgt gctcgacgag ccgaccacgg gtctgcacct cgccgacgtc gagcaactgc
     4621 tcgggctact ggaccggctg gtcgactccg gcaagaccgt gatcgtgatc gaacaccacc
     4681 aggcggtgat ggcgcatgcc gactggatca tcgacctcgg gccgggcgcg ggccacgacg
     4741 gcggccggat cgtcttcgag ggcacgccgg ccgaacttgt cgccgcccga tctaccctga
     4801 ccggccagca cctcgcagcc tacgtcgggg cctgaccggg tctgtttcgt ccacgcggga
     4861 tcgggcggct gctatgctca ctcgcatggg gaaacagcga caaaccgtcg gcatcatcgg
     4921 cgcgggtcag ctcgcccgga tgctggtcga agccgcgaca ccgctcgata tcgacatcgc
     4981 gctccttgcc gccgctgata ccgatggagc ggcgcggatc tggccgaacg tgacgatcgg
     5041 ctcgcccgac gacgctgagg cggttgccgc gttcgccgcg acctgcgacg tggtcacctt
     5101 cgaccacgag ctggtgccgg aaccggtcct cgcgcggctc gaagcggcgg gcgtatcgct
     5161 cgcgccgagc gctgacacga tgcggatctc
//
LOCUS       DASSGX010000619         3338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_107290, whole genome shotgun sequence.
ACCESSION   DASSGX010000619 DASSGX010000000
VERSION     DASSGX010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107290"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="VFI12_11210"
     CDS             complement(<1..584)
                     /locus_tag="VFI12_11210"
                     /inference="COORDINATES: protein motif:HMM:NF014247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HET7057024.1"
                     /translation="MRKTLGAVLAVGLLAVTAQSAPAAEAVHIERQSWPFAGFFGTFD
                     RAQLQRGFQVYTEVCASCHELNRISYRNLAQPGGPEFPEDAVAALAAAAQVEDGPNDQ
                     GEMFTRPGRLSDPLPKLYGNENEARAIHNGAYPPDLSLMARARNTENVAPWYIHPFLM
                     LRDIAVGYQEGGADYVYALLTGYEEPPAGFELMDG"
     gene            complement(620..1903)
                     /locus_tag="VFI12_11215"
     CDS             complement(620..1903)
                     /locus_tag="VFI12_11215"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HET7057025.1"
                     /translation="MATHQSTYEPKSGVGKWMDERLPVARMMHGQFVDFPTPRNLNYL
                     WTFGGILTFVLGVQILTGIVLAMHYIPSAADAFDSVERIRRDVNYGWLIRNLHAVGAS
                     MFFFAVYVHIFRGLYYGSYKAPREVLWIIGVIIYLVMMATAFLGYTLPWGQMSFWGAT
                     VITNFFSAIPLVGESVVQWLWGGYAVSGVTLNRFYSLHYLLPFVLVGLVALHIWALHV
                     TGQNNPTGIEPQTKSDTVPMSPYAVSKDAVGMIVFLILFAWFVFFVPDYLGHADNYNQ
                     ANPLVTPPQIVPEWYFLPFYAILRAIPSKLGGVIAMFGAIAILVFVPWLDTSRVRSTK
                     YRPLYRWFFWAFVFTCLALGYLGAKPAEGVYVTWARVFTLYYFAHFLVVMPIIGVIET
                     PTAMPRSISEAVLGKKGNGGSAVSPATATAAAEKR"
     gene            complement(1918..2454)
                     /gene="petA"
                     /locus_tag="VFI12_11220"
     CDS             complement(1918..2454)
                     /gene="petA"
                     /locus_tag="VFI12_11220"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HET7057026.1"
                     /translation="MSAHAEEPTRRDFLTIAGGAFAAVGGAMALWPFIAQMNPDASTQ
                     ALASVEVDLAPVQPGQAITVMWRGKPVFIRNRTPEEIENARATPVGDLPDPETDRVRV
                     KSGHDNWLVLVGICTHLGCIPKGQSLNDMKGDYGGWFCPCHGSHYDLSGRIRKGPAPR
                     NLEVPTYEFVSDSKIRIG"
     gene            complement(2828..>3338)
                     /locus_tag="VFI12_11225"
     CDS             complement(2828..>3338)
                     /locus_tag="VFI12_11225"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET7057027.1"
                     /translation="DVLHRESLETLLVKYGVGGLSEAEKDHLTRVWHRLSPWPDSVPG
                     LLRLKSRYLIGTLSNGNVGLLAWMAKNAGLPWDVVLGAGTARAYKPLPEAYHRTADML
                     GLGPGQVMLVAAHNDDLASAAAQGYATAFVARPTEYGPRQMKDFKAERDWDVVTDSFL
                     GLADALGCP"
BASE COUNT          621 a         1067 c         1099 g          551 t
ORIGIN      
        1 ccgtccatca gctcgaaccc ggccggcggc tcctcgtagc cggtgaggag cgcatagacg
       61 tagtcggccc cgccctcctg gtagccgacc gcaatgtcgc gcagcatcag aaacgggtga
      121 atataccagg gcgcgacgtt ctcagtgttc cgcgcccgcg ccattagcga caggtcgggg
      181 ggataggcgc cgttgtggat cgcgcgcgcc tcgttctcat tgccgtaaag cttcggcaac
      241 gggtccgaca ggcggccggg gcgcgtaaac atctcgcctt gatcattggg cccgtcctcg
      301 acctgcgctg ccgcagcaag ggctgccacc gcatcctccg ggaactccgg cccgcccggc
      361 tgggcgaggt tgcggtacga gatgcggttg agctcgtgac agctcgcgca cacctcggtg
      421 tagacctgaa agccgcgttg aagctgtgcc cggtcgaagg tgccgaagaa gccggcgaac
      481 ggccagctct gccgctcgat gtggaccgcc tcggcggccg gggcagactg ggcggtcacg
      541 gccagcagac ccacggcaag caccgccccc agagtttttc tcattgtgat cattgcctcg
      601 aacctcgcct ccccgacctt caccgcttct cggccgctgc cgtcgccgtc gcgggggaaa
      661 ccgcgcttcc gccattgccc ttcttgccca ggacggcctc gctgatggag cgcggcatcg
      721 cagtcggcgt ctcgatcacg ccgatgatcg gcatcacgac gaggaagtgc gcgaagtagt
      781 agagcgtgaa cacgcgggcc cacgtcacgt agacaccttc cgccggcttc gcaccgaggt
      841 agcccagcgc gaggcaggtg aacacgaagg cccagaagaa ccagcggtaa agcggccggt
      901 acttcgtgga ccgcacgcgc gacgtgtcga gccagggcac gaacacaagg atggcgatgg
      961 cgccgaacat tgcgatcacg ccgccgagct tggacggaat ggcccgcagg atcgcgtaga
     1021 acggcaggaa gtaccattcc ggcacgattt gtggcggcgt caccagcgga ttggcctgat
     1081 tgtagttgtc cgcatgcccg aggtagtcgg gaacgaagaa cacgaaccac gcgaacagga
     1141 tcaggaacac gatcatgccc acggcatcct tcgagaccgc gtaggggctc atcggcaccg
     1201 tgtcggattt cgtctgcggc tcgatgcccg tcgggttgtt ctgaccggtc acgtgcagcg
     1261 cccagatgtg gagcgcgacg aggccgacga gcacgaaggg cagcagataa tgcaggctgt
     1321 agaagcggtt gagcgtcacg cccgacaccg cgtagccgcc ccacagccac tgaacgacgc
     1381 tctcgccgac cagcgggatg gccgagaaga agttcgtgat gacggtggcg ccccagaagc
     1441 tcatctgccc ccacggcagg gtgtagccga ggaacgcggt ggccatcatg acgaggtaga
     1501 tgatgacgcc gatgatccac agcacctcgc gcggcgcctt gtaggagccg tagtagaggc
     1561 cgcggaagat gtgcacatag acggcgaaga agaacatcga ggcgccgacc gcgtgcaggt
     1621 tgcggatcag ccagccgtag ttcacatccc ggcggatgcg ctcgacggag tcgaaggcat
     1681 cggcagcgct cgggatgtag tgcatcgcga gcacgatgcc tgtcaggatc tgaacgccca
     1741 gcacgaacgt caggatgccg ccgaaagtcc agaggtaatt gagattgcgc ggggtcggga
     1801 aatcgacgaa ctgcccgtgc atcatgcgtg caacggggag ccgctcatcc atccatttgc
     1861 caacgccgga cttcggttcg taggtcgact gatgtgtagc catggtgtcc gtgatcctca
     1921 gccgatcctg atcttggaat ccgatacgaa ctcgtaggtt ggcacctcga gattgcgcgg
     1981 cgccgggcct ttgcgaatgc gccctgacag gtcgtagtgg gagccgtggc acggacagaa
     2041 ccagccgccg taatccccct tcatgtcgtt gaggctctgg cccttgggga tgcagccgag
     2101 atgcgtgcag atgccgacca ggacgagcca gttgtcatgc ccgctcttca cacgcacgcg
     2161 gtcggtctcg gggtctggaa ggtcgcccac cggagtcgcc ctcgcgttct cgatttcttc
     2221 cggcgtgcga ttgcggatga agaccggctt gccgcgccac atgacagtga tagcctgtcc
     2281 gggctgcacg ggagccaaat cgacctcgac cgaggcgaga gcctgtgtcg atgcatccgg
     2341 attcatctgc gcgatgaacg gccacagcgc catggcgcca ccgacggccg cgaaagctcc
     2401 ccccgcgatc gtcaggaagt cccgacgcgt tggctcctcg gcgtgtgcgc tcaattgagc
     2461 ctcccttcta cgtgcgaatg acccgaatcc cctgcgatcc gggcactcct tgaacggccg
     2521 tcgcaccggt cctactgccg cgagcgccca gctccccacg gagtggccgt atcgaacagt
     2581 cgccatctat aagtcggcaa atcccccgct cagaacggcg ctttttgcca cgccgacctt
     2641 ttgacaccga tcgtcaacgg cctcaaggcg cgggagcgcg gcggcaaaga ggcaatttgg
     2701 cgcatattgg gacaggcggg gacgcttctg gaagcggcgg gtagctcgag attgtgagca
     2761 ggggttcggc agtggccagt cggcaatcgt ggggctcggt gggctgagcg cagtggcgtg
     2821 ctgcggatca ggggcagcca agcgcgtcgg cgaggccgag gaagctgtcc gtcacgacgt
     2881 cccagtcccg ctcggccttg aagtctttca tctgacgagg cccgtactcc gtcgggcgtg
     2941 cgacgaaggc ggtagcatag ccttgggccg ccgcggaggc gagatcatcg ttgtgggcag
     3001 ccaccagcat cacctgaccc ggcccgagac ccagcatgtc cgcggtgcga tggtaggctt
     3061 cgggcagcgg cttgtaggcg cgcgccgtcc cggcgccgag aacgacatcc catggcaggc
     3121 cggcgttctt cgccatccat gccagcagac cgacgttgcc gttcgacagc gtgccgatca
     3181 ggtagcgcga cttcaggcgc agaagcccgg gcacactgtc cggccacggc gagaggcggt
     3241 gccaaacccg agtgaggtga tccttctcgg cctccgatag ccctccgaca ccgtacttga
     3301 cgagcagcgt ttcgaggctc tcccggtgca gcacgtcg
//
LOCUS       DASSGX010000620         2873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1414464, whole genome shotgun sequence.
ACCESSION   DASSGX010000620 DASSGX010000000
VERSION     DASSGX010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414464"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="VFI12_11230"
     CDS             <1..797
                     /locus_tag="VFI12_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7057028.1"
                     /translation="SPEAMASPEAAPALASTPADEATTQAAVAAADNIAACANDPAAL
                     ASLVTANLVQTHPFGGFPTIAEAMANGFFEGVPYAAGVKVGDVVTYEDGSIGAKVTYM
                     QSQYQAVGELWNLVDVDGTWKLNAVANAEPDIESADFTAVVGAKLGENEDGTYYLTPS
                     RESVTEASVLVLQGTNEGQEPHEMVMVQLPEGVDPMGLLDGSVSEDQVNFIGFVFMPH
                     TGDIANMTLVNLPAGKYTLLCFIPGPDGAPHAAHGMISPFEVTAAE"
     gene            873..2723
                     /locus_tag="VFI12_11235"
     CDS             873..2723
                     /locus_tag="VFI12_11235"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET7057029.1"
                     /translation="MSQQSGPFAALFEEYKAGRVSRREFMRRAVMLGVGAPVALYVMN
                     AVDPTIAHAQDATPAAAGTLKAPDAGTEGQTRGAGGELKLAQGQGITHFGLHTATGTK
                     DQLGASLVTEPLLNTLPDGNLIPCLAAEVPSIANGGLAEDLSSVTYKLKEGVLWSDGQ
                     PFTADDVVFTWQWITNEANGSIDIKTYQIISNVEAIDPLTVKVTFTSPQIAWYVPFTS
                     SYLGSIYPKHVLEAGTDAYNAFILKPTGTGPYMVDSFTVNDQAIYKVNPNYREANKPF
                     FETVNVKTQEDAIRSVLQTGDWDFAWNVQVEPDIQKSLEANGKGTVKALSPAAVERLL
                     INFSDPNKEVDGQRSQKDTPHPFFSDLKVRQAFALATDRDTIANKFYMGGTAEPPAKN
                     ILTGIAILESPNTSYEFNLDKAAALLDEAGWALDGKTRKKDGMELKVDYSTTINAVRQ
                     KTQLVNKQNWESIGIKVNLKTVDPGIFFDSAAGNELNAQHFYADLLMYTNNPSSTFPV
                     SYMQSWYGGKDGLNIAQAPDWSGINESRYHSEEYDALYDQVTTETDPEKVAGLFIAMN
                     DHLINNQVIIPEVARAAEKYAIINSLNDANIAGSIFEALYWNIANWNRVQ"
BASE COUNT          586 a          981 c          852 g          454 t
ORIGIN      
        1 catcgcctga ggccatggcc tcgccggaag ccgcgccggc cctggccagc accccggccg
       61 acgaggcgac gacccaggca gcggtcgcag cggccgacaa catcgcggcc tgcgcgaacg
      121 acccggccgc actcgcgtca cttgtgacgg cgaatctcgt ccagacacac ccgtttggcg
      181 gcttcccgac gatcgccgag gccatggcca acggcttctt cgagggcgtt ccgtatgcgg
      241 ccggcgtcaa ggtcggcgat gtcgtcacct atgaggacgg cagcatcggc gccaaggtca
      301 cctacatgca gtcgcagtat caggcggtcg gcgagctgtg gaacctcgtt gatgtcgacg
      361 gcacctggaa gctgaatgcg gtcgccaatg ctgagccaga catcgagtcg gcggacttca
      421 cggcggtcgt gggcgccaag cttggtgaga acgaggacgg cacatactac ctgacgccaa
      481 gtcgggagtc tgtcactgaa gcgagcgttc tcgtcttgca gggcacaaac gagggccagg
      541 agccgcacga gatggtcatg gtccagctgc cggaaggcgt ggatccgatg ggcctgctcg
      601 atggctccgt ctccgaggac caggtcaact tcatcggttt cgtcttcatg ccgcacaccg
      661 gcgacattgc caacatgacg ctggtcaacc tcccggcggg caagtacaca ctgctctgct
      721 tcatcccggg cccggatggc gcgccgcatg ccgcccacgg catgatctcg cccttcgaag
      781 tgacggcggc tgaataacgc ggaacttccg ccggcgatca atcgcgatcg ccggcgatgt
      841 tcataagaat gaacgctcag gaggaacagt ccatgtcgca gcagtctgga cctttcgcgg
      901 ccctgttcga ggagtacaag gcgggacgag tctcccgccg cgagttcatg cgtcgtgcgg
      961 tcatgctcgg cgtcggcgcg cctgtcgcgc tctacgtcat gaatgccgtc gacccgacca
     1021 ttgcccatgc ccaggatgcc acgccggcgg ccgccgggac cctgaaggcg ccggatgccg
     1081 gcaccgaggg ccagacccgg ggcgcgggcg gcgagctcaa gctcgcccag ggccagggca
     1141 tcacccactt cggtctccac accgcaaccg gcaccaagga tcagcttggc gccagcctcg
     1201 tcaccgagcc gctcctgaac accttgccgg acggcaatct gatcccctgc ctggccgcgg
     1261 aagttccgtc gatcgccaac ggcggccttg ccgaggatct cagctcggtc acctacaagc
     1321 tcaaggaggg cgtcctctgg agcgacgggc aaccgttcac cgcggacgac gtcgtcttca
     1381 cgtggcagtg gatcaccaac gaggccaacg gctcgatcga catcaagacg tatcagatca
     1441 tctcgaacgt cgaggcgatc gacccgctca cggtcaaggt caccttcacc tcgccgcaga
     1501 tcgcatggta cgtgccgttc accagctcct acctcggctc gatctatccg aagcatgtcc
     1561 tcgaggccgg cacggacgcc tacaacgcgt tcatcctgaa gccgaccggc accgggccgt
     1621 acatggttga ctccttcacg gtcaatgacc aggcgatcta caaggtcaac ccgaactacc
     1681 gcgaggcgaa caagccgttc ttcgaaacgg tcaacgtcaa gacccaggaa gacgccattc
     1741 gctccgtcct ccagacgggc gactgggact ttgcctggaa cgttcaggtc gagccggata
     1801 tccagaagtc cctcgaagcc aatggcaagg gcaccgtcaa ggctctcagc ccggcagccg
     1861 tggagcggct cctgatcaac ttctccgacc cgaacaagga ggtcgacggc cagcggtccc
     1921 agaaggacac gccgcacccg ttcttctccg atctcaaggt ccgccaggcc ttcgcgctgg
     1981 cgaccgaccg cgacacgatc gccaacaagt tctacatggg cggcaccgcg gagcccccgg
     2041 cgaagaacat cctgaccggc atcgccatcc tcgagagccc gaacacctcc tacgagttca
     2101 acctcgacaa ggcggcggcc ctcctcgacg aggccggctg ggcgctcgac ggcaagacgc
     2161 gcaagaaaga cggcatggag ctcaaggtcg actactcgac cacgatcaac gccgtccgcc
     2221 agaagaccca gcttgtcaac aagcagaact gggagtcgat cggcatcaag gtcaacctca
     2281 agacggtcga cccgggcatc ttcttcgaca gcgcggccgg caacgagctg aatgcccagc
     2341 acttctatgc cgacctgctg atgtacacca acaacccgtc cagcactttc ccggtctcgt
     2401 acatgcagtc gtggtatggc ggcaaggacg gcctgaatat tgcccaggca ccggactggt
     2461 ccgggatcaa cgagtcgcgc taccacagcg aggagtacga cgccctctac gaccaggtca
     2521 cgaccgagac cgacccggag aaggtcgccg gcctcttcat cgcgatgaac gaccacctga
     2581 tcaacaacca ggtgatcatt ccggaagtcg cccgcgcggc cgagaagtac gcgatcatca
     2641 actcgttgaa tgacgcgaac atcgccggct cgatcttcga ggccctctac tggaacatcg
     2701 cgaactggaa ccgcgtccag taagacgcgc tccattcgag ctcacgagaa cggggtggcg
     2761 attgctcgcc accccgctct catgcccaac gcctccgccg gagactcaag tccccggctc
     2821 accgatggaa gtcggctgat ggtcattgat ggaccggctc ggtagtggga acc
//
LOCUS       DASSGX010000621         3494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1085317, whole genome shotgun sequence.
ACCESSION   DASSGX010000621 DASSGX010000000
VERSION     DASSGX010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1085317"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1463)
                     /locus_tag="VFI12_11240"
     CDS             complement(<1..1463)
                     /locus_tag="VFI12_11240"
                     /inference="COORDINATES: protein motif:HMM:NF026092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HET7057030.1"
                     /translation="MSHRVELRPRPTERSCYSGRSLLVTNLDGWVAGSGLEGFYVDET
                     RLLSRREVTISGMPLTGVATSPVGGTRLLSYAEAPEQPSVPAHAVYIEDACRVGDGLR
                     EELRFHNYSAREATSFELGIHLGADFADLFEAKDGTRQQTGPVETCWDAGRQELMFRY
                     GHPGLDRAVAVRIESAPAPVRCEEATLLIPIDLAPREATEIHLVVEPIFDGVRRSAPD
                     RFAGPSDEPLDRIRRQLGREAPALTTTNPTVARAWRTAVADLASLPLGLEGGPAAPIA
                     GIPFYQQFFGRDMLTISWQALLALPSMMRDSLRLNAAWQGTVIDNWLDEEPGKMIHQA
                     RWGPLSILGIDPFIRYYGDYATAPDFLVMLSQYLAWTNDIDTVRELLPAARKAIGWLD
                     DYADLDGDGFIEYVTRSEKGVKNQGWKDSGDAIIDERGEVIENPIATCELQAYWYAGL
                     ELGAAAFFIAGDRAYALELMGKARDLKRRFNRAFWME"
     gene            complement(1789..2325)
                     /locus_tag="VFI12_11245"
     CDS             complement(1789..2325)
                     /locus_tag="VFI12_11245"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HET7057031.1"
                     /translation="MESRAKLFGHPIHQQLIVFPLGLFGMGTLFDIIDWVADEPQLAR
                     AAYYMIAAGILSGLLAAIFGLIDYLAIPSGTRAKRIATTHGVGNVVIVVLFIIAWFLR
                     RDDPAAPSTFARILEIIGLVMALGTGWLGGELVDRLGVGVDPGANLNAPSSLSGPAAA
                     GSGPGVARSREAGLRVES"
     gene            complement(2555..3139)
                     /gene="folE"
                     /locus_tag="VFI12_11250"
     CDS             complement(2555..3139)
                     /gene="folE"
                     /locus_tag="VFI12_11250"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HET7057032.1"
                     /translation="MTDEQMHRFEGYAAEIFSAFGMDIDTPSTVDTPRRFIQAMYDAT
                     AGYDGDPKLITVFETECRGGSDCRLSQVIEGPINFFSLCEHHALPFRGRAYVGYIAHE
                     HIIGISKLTRLVRLYARRFSVQERIGQQIADTLEAMLEPHGVAVYLEAHHLCMQMRGV
                     QEPASLTRTTFWRGEYDANPALRSEFLAACGLNR"
     gene            complement(3241..>3494)
                     /locus_tag="VFI12_11255"
     CDS             complement(3241..>3494)
                     /locus_tag="VFI12_11255"
                     /inference="COORDINATES: protein motif:HMM:NF026109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase C-terminal domain-containing
                     protein"
                     /protein_id="HET7057033.1"
                     /translation="PLFYSDLFDMGYEAVGEVDTRRETVADWAEPNRKGVIYYLGDGK
                     VRGVLLWNVWEKVDAARELIKAGEQVTAESVRGKIGMGE"
BASE COUNT          600 a         1183 c         1132 g          579 t
ORIGIN      
        1 tcctccatcc agaacgcgcg attgaatctc cgcttgaggt cgcgagcctt gcccatcagc
       61 tccagggcat aggcccggtc gccagcaatg aagaatgcgg cagcgccgag ttcgagcccg
      121 gcgtaccaat aggcttgcag ctcgcaggtt gcgatcgggt tctcaatgac ctccccgcgc
      181 tcgtcgatga tggcatcgcc cgagtccttc cagccctggt tcttgacgcc cttctccgac
      241 cgcgtcacgt attcgatgaa gccgtctcca tccagatccg cgtagtcgtc cagccacccg
      301 atcgccttcc gcgccgccgg cagcagctcg cgcacggtgt cgatgtcgtt cgtccaggca
      361 aggtactggg agagcatgac caggaagtcg ggcgcggtcg cgtagtcgcc gtagtagcgg
      421 atgaagggat cgatcccgag gatcgagagt ggtccccagc gcgcctggtg gatcatcttg
      481 cccggctcct cgtcgagcca gttgtcaatc accgtgccct gccaggccgc gttcaggcgg
      541 agactgtccc gcatcatcga gggcagagca agcagggctt gccaggagat cgtcagcatg
      601 tcccggccga agaactgctg gtagaaggga atgccggcga tcggcgcggc cggaccgccc
      661 tccaggccga gtggcagcga ggcgaggtcg gcgaccgcgg tccgccaggc gcgcgccacc
      721 gtcggattcg tcgtggtcag cgccggcgcc tcgcgtccca gctgccggcg aatgcgatcc
      781 aggggctcgt cggacggacc ggcaaagcgg tccggtgccg aacgacgaac gccatcgaag
      841 atcggctcga cgaccaggtg aatctcggtc gcctcgcggg gcgcgagatc gatcgggatc
      901 aggagcgtcg cctcctcgca gcgaaccggc gccggcgcgc tctcgatccg gacggcgact
      961 gcccggtcca gtccaggatg cccatagcgg aacatcagct cctggcggcc ggcatcccag
     1021 caggtctcca ccgggccggt ttgctgccgc gtgccatctt tcgcctcgaa gaggtcggcg
     1081 aaatcggcgc cgaggtgaat gccgagctcg aagctggtcg cctcgcgggc gctgtagttg
     1141 tggaagcgaa gctcctcgcg cagaccgtcg ccaacgcggc acgcatcctc gatgtagacg
     1201 gcatgggccg gaaccgacgg ctgctcgggg gcctcggcgt aggagaggag ccgggtgccg
     1261 ccgaccgggc tggtcgcgac cccggtcagc ggcatgccgg aaatcgtgac ctcccgccgg
     1321 ctgaggagcc gcgtctcatc gacgtagaag ccctcgaggc cgctgccggc cacccagcca
     1381 tcgaggttgg tgacgaggag cgagcggccg ctgtagcagg agcgctctgt tggacgcggg
     1441 cgtagctcaa cgcggtggct catggtccta tcgttcgacc tgcccgacgg gggccggcat
     1501 cccgggcagg tctcccggga ttgcaacccc gggctgagaa cacgaacgct ctccgaggct
     1561 gatcgggaac cgggtccggc atcatcaatc cccgaccgag agcaccaagc gggtacatcc
     1621 cctctccgac gctccccaat tgggagagag catctccgcc accgcggtgc gcccaagccg
     1681 gcttcaaccg ggttccggtt tcagcccgtt gcttcagcgc cggggatgcg tgccggccgg
     1741 gagggggcgg gcagatggca tctgcccgta tcccgcctcc ctcaactctc aactctccac
     1801 ccgcaaccca gcttcgcgac tccgcgccac tccggggcca ctgcccgcgg ccgccgggcc
     1861 tgacagcgag ctcggcgcgt tcaggttcgc gcccggatcg acgccgacgc cgagccggtc
     1921 gaccagctcg ccgccgagcc accccgtccc aagcgccatg accaggccga tgatctcgag
     1981 gatccgggca aaggttgacg gcgccgccgg atcatctcgt cgcaggaacc aggcgatgat
     2041 gaagagcacg acgatcacga cgttgccgac gccgtgcgtg gtggcgatgc gcttcgcccg
     2101 ggtgccggac gggatcgcga gatagtcgat caggccaaag atggcagcga gcaaaccgga
     2161 gaggataccg gccgcgatca tgtagtaggc ggcccgggca agctgcggct cgtcggcgac
     2221 ccagtcgatg atgtcgaaga gggtgcccat cccgaacaat ccgagcggga agacaatcag
     2281 ttgctggtga atcgggtgac cgaacaattt ggctctgctt tccatcgcaa tgaacctccg
     2341 ttcctcatac cgctattccc cggtcaatcg cggcgccgaa gttggtctgc ctccgcgtgg
     2401 cagcatgcct tggcacgctc cagcatcccg ccatttgacc tgaccattgt tatgccaatc
     2461 agatcagcaa atcggtcgat tcgcgtgccc aggccacaat tcgcgaccca tccagcaaat
     2521 cgatcaacga tgctcactcg gatctcctct ccccttagcg gttcagaccg caggccgcca
     2581 aaaactcgct ccgcagtgcc gggttggcgt cgtactcgcc gcgccagaat gtcgtccggg
     2641 tcagcgaggc cggctcctgg acgccgcgca tctgcatgca gaggtgatgc gcctcgaggt
     2701 agaccgcgac gccgtgcggc tcgagcatcg cctccagcgt gtcggcaatc tgctggccga
     2761 tccgctcctg gacggaaaaa cggcgggcat agagccggac cagccgggtc agcttcgaga
     2821 tgccgatgat gtgctcgtgc gcgatgtagc cgacgtaggc tcggccacgg aacggcagcg
     2881 cgtggtgctc gcagagggaa aagaagttga tcggtccctc gatgacctgg ctcagccggc
     2941 aatccgaccc accccggcac tcggtctcga agaccgtgat cagcttcgga tcgccgtcgt
     3001 agccggcggt cgcgtcgtac atcgcctgga tgaagcggcg cggggtgtcg accgttgacg
     3061 gcgtatcgat gtccatgccg aacgcgctga agatctcggc cgcgtaccct tcgaagcggt
     3121 gcatctgctc gtcggtcacg gaccgccgct ggactcggac gggggaggct ggcacaaccg
     3181 cgtggctgtg cccgtgcccg tgcccgtttg tggaaggtgc aattacggtg ctcatcgatt
     3241 tcactcgccc atcccgatct tgccgcggac cgattcggcc gtaacctgct cgcctgcctt
     3301 gatcaattcc cgcgcggcgt ccaccttctc ccagacgttc cagagcagga caccccgcac
     3361 tttgccgtcg ccgaggtagt agatgacgcc cttgcggttc ggttcggccc aatcggccac
     3421 ggtttcgcgc cgcgtatcga cttcgccgac cgcctcgtag cccatgtcga agaggtccga
     3481 gtagaagagc ggca
//
LOCUS       DASSGX010000622         3646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_108060, whole genome shotgun sequence.
ACCESSION   DASSGX010000622 DASSGX010000000
VERSION     DASSGX010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108060"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..1001)
                     /gene="thrB"
                     /locus_tag="VFI12_11260"
     CDS             complement(96..1001)
                     /gene="thrB"
                     /locus_tag="VFI12_11260"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HET7057034.1"
                     /translation="MRFSVRAPASSANLGPGFDALGLALDLWNSIEIETGGEGIRVEY
                     AGSDAKGMDGNENYSTRAMRLLAEEHGRTLPPFRLTAQIDVPVARGLGSSAAAIVAGL
                     IAANHMLDLGLGDDRIFQAAARMEGHGDNVGAALHGGAILSVPGLGRAVKLSDGIDLD
                     LTTVVYVPDEHGATWVARAELPQHVTLADAAFNVGAAGGIVAGLITGDVDAIAAGMHD
                     RLHEPPRSRLYPHLIPMAEAARSAGAIGACLSGAGPTVLALVTHERMPEVCAAMQDAG
                     HTAGIPGHCQHLPISATGAHILADK"
     gene            complement(991..2247)
                     /gene="thrC"
                     /locus_tag="VFI12_11265"
     CDS             complement(991..2247)
                     /gene="thrC"
                     /locus_tag="VFI12_11265"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET7057035.1"
                     /translation="MDCGKTFTANRPLTACPACGGLLDAVIDTSRPIDRSAFGTGLSP
                     ALAQSGVWRYRQLLPQIPDTFIVTRAEGRTPIYWDDRIAQYAGIGSMGLKHEGQNPTA
                     SFKDRGMTVGMSHAMAVGARFVACASTGNTSASLASYAASANIPALVLIPEGKISGGK
                     LAQTLAHGALVVQIEGDFDAALALLRQVAADYDRYQVYLVNSVNPFRPEGQKTTVFEL
                     LEQLDWDIPDVIALPGGNLGNTAAFGKALTELYETKLISRMPRLATIQAAGAAPFAAY
                     YESGWDHFTPVEAETIATAIKIGNPASTARAMRAIISTGGVVTSVTDDEIMDAKAVID
                     RVGIGCEPASAASLAGVRKLVAAGEIKPGETVVGILTGHVLKDTDAVTNYHLGAGSQD
                     RPLANRPTSIPASLDALEKVLSAHAV"
     gene            complement(2486..3151)
                     /locus_tag="VFI12_11270"
     CDS             complement(2486..3151)
                     /locus_tag="VFI12_11270"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class F sortase"
                     /protein_id="HET7057036.1"
                     /translation="MNDSIRINRRDLARFGLGALALGAAGFGPARLLAAPKIESTQRG
                     EMPTTGGLRPGPLGLSPQPLTVAGFTPVAISIEKAGVAAEVETREIVDGVMQDPTGPW
                     VVSWYKETGRLGVPDENVVMAAHVDYWDVGPAVFYNIRVLAEGDPVDVTGDDGNVYRY
                     AVEWQKLFDADNAPIQEIVGPTDESALTLITCGGQFDYANGHYISRTVVRCKFAEKVA
                     ATS"
     gene            complement(3348..>3646)
                     /locus_tag="VFI12_11275"
     CDS             complement(3348..>3646)
                     /locus_tag="VFI12_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7057037.1"
                     /translation="MLAAHEAGLICLTGGASGPVGQLLLRGDRAGAEALFQSELPYVL
                     WAMQSIDFSVRATKTELLVRRVIQTKAMREPWGHLDATAEAQLARFIAERPGNT"
BASE COUNT          599 a         1236 c         1204 g          607 t
ORIGIN      
        1 ccggcaggtc gtcaccgaag ccgggcgggc cgcccgcggc tacacacaaa gaggcccacc
       61 cgtctcaata aggttgttgt cggactcgag caggttcact tatcggcaag aatgtgtgcg
      121 cccgttgccg agatggggag atgttggcag tggccgggga tgcctgccgt gtggccggcg
      181 tcctgcatgg ccgcgcagac ctccggcatt cgctcgtgcg tgaccagagc gagcacggtc
      241 ggacccgcgc cactcaggca ggcgccgatc gccccggccg aacgcgccgc ttcggccatc
      301 ggaatcaagt gcggatagag gcgggagcgc ggcggctcgt gcagtcgatc atgcattccg
      361 gcagcgatcg cgtcgacatc gcccgtgatc aacccggcca cgatcccacc ggcggcgccg
      421 acattgaacg ccgcatccgc cagcgtgacg tgctgcggca gctcggcccg cgccacccag
      481 gttgcgccgt gctcatccgg gacgtagacg acggtggtca ggtccaggtc gattccatca
      541 gagagcttca ccgctcggcc gagacccggc acggagagga tcgcgccgcc gtgcagtgcc
      601 gctccgacat tgtcgccgtg tccctccatt cgcgccgccg cctggaaaat ccggtcgtca
      661 cccaggccaa gatcgagcat gtgattggcg gcgatcaggc cggccacgat tgccgcggcc
      721 gagctgccaa gaccgcgtgc gaccggcaca tcgatctgcg cggtcaggcg aaagggcggc
      781 agggtgcgac cgtgttcctc ggccagcagc cgcatcgccc gcgtcgagta gttctcgttg
      841 ccgtccatgc ccttggcatc ggaaccggcg tattcgaccc ggatgccctc accgccggtc
      901 tcgatctcga tcgagttcca gaggtcgagc gccagcccga gcgcatcgaa gccgggacca
      961 aggttggcag atgaggcagg ggcccgaacg ctaaaccgca tgcgcgctca gcactttctc
     1021 cagcgcgtcg agcgacgccg ggatcgatgt cggccggttc gccagcgggc ggtcctgcga
     1081 ccccgcgccg aggtgataat tggtcaccgc gtccgtgtcc ttgaggacat ggccggtcag
     1141 gatgccgaca acggtctcgc ccggcttgat ctcacccgcg gcgaccagct tacgcacgcc
     1201 ggccaggctc gcagccgacg cgggctcgca gccgatgccg acccggtcga tgacggcctt
     1261 ggcgtccatg atttcgtcgt ccgtgaccga ggtgaccacg ccgccggtcg agatgatcgc
     1321 ccgcatcgcc cgggccgtgc tggccgggtt gccgattttg atcgcggtcg caatcgtctc
     1381 ggcctcgacc ggcgtgaagt ggtcccagcc cgactcgtaa taagcggcga acggcgcggc
     1441 gccggcagcc tggatcgtgg cgaggcgcgg cattcggctg atcagctttg tctcgtagag
     1501 ctcggtgagc gccttgccga aggcggcggt attgccgaga ttgccgcccg gcagcgcgat
     1561 cacgtccggg atgtcccagt ccaattgttc gagcagctcg aagacggtcg tcttctggcc
     1621 ctccggccgg aacggattga ccgagttgac gaggtagacc tggtaccggt cgtagtcggc
     1681 cgcgacttgc cggagcaggg ccagggcggc gtcgaaatcg ccctcgatct ggacgaccag
     1741 cgccccgtgc gccagcgttt gcgccagctt cccgcccgag atcttgccct ccgggatcag
     1801 gaccagcgcc gggatattcg ccgatgccgc atacgacgcc agcgaagcag acgtgttgcc
     1861 ggtcgaggcg caggccacga agcgcgcgcc gacggccatg gcgtgactca tcccgacggt
     1921 catgccccgg tccttgaacg acgcggtcgg gttctgcccc tcgtgcttga gccccatcga
     1981 gccgatgccg gcatactgcg cgatccggtc gtcccaataa atcggcgtcc gtccctcagc
     2041 gcgggtgacg atgaacgtat ccgggatttg cggaagcaac tgccggtagc gccaaacgcc
     2101 ggattgcgcc agggcgggcg agagccccgt gccgaatgct gaccggtcga tcggccggct
     2161 cgtgtcgatc acggcgtcga gtaggccgcc gcaggccggg caggcggtca agggccggtt
     2221 cgcggtgaac gttttgccgc aatccatgca gcggaccgaa agttgtgcga gcgcgtcatc
     2281 ggaaccggcg cgcgaagaat cctggatcag gctcacagcc cctcccaaaa gccacccaac
     2341 atcatccatt gacggagtgc gtacccgagc ggataaaccc ttcgcgcagc ataacgcaga
     2401 tcattgcccc gggtcccgga gagctatgag aaaccggcag gcgagtcgcc tgccggttag
     2461 aggtcgtgct gtttgatgta tgcgcttacg aggtcgcggc caccttctcc gcgaacttgc
     2521 agcggaccac cgtgcgggag atgtaatgac cgttcgcata gtcgaactgg ccaccgcagg
     2581 tgatcagtgt cagcgcgctt tcgtcggtcg gaccaacgat ttcctggatc ggcgcgttgt
     2641 cggcatcgaa cagcttctgc cattcgaccg cgtaccggta gacgttgccg tcatcgccgg
     2701 ttacatcgac gggatcgccc tcggccagga cacggatgtt gtagaagacg gccgggccga
     2761 catcccagta gtcgacgtgc gccgccatca cgacattctc gtccggcacg ccgagtcgcc
     2821 cggtctcctt gtaccaggag acgacccagg ggccggtcgg gtcctgcatg acgccgtcga
     2881 cgatttcccg cgtctcgact tccgctgcca ccccggcctt ttcgatcgag atcgcgaccg
     2941 gcgtgaagcc ggcgaccgtc agcggctgcg ggctgaggcc gagcgggccg ggccggagtc
     3001 cacctgtggt cggcatctcg ccacgctggg tggattcgat tttcggagcc gccagcaggc
     3061 gcgccgggcc gaagccggcc gcgccgagcg ccagcgcgcc gagaccaaac cgggccagat
     3121 cgcggcggtt gattcgaatc gagtcgttca tcagtgcctc cctgagggtg gacgaaccgg
     3181 gtgtgctgcg cggtccgtgg ctaggcgtcg gggagaatcg gctcgcgcgt tgctagtctg
     3241 catcctctaa cgcatcgagc gctgaaatcg tatcactcgg taactccgac gcgtctgatc
     3301 tcctgtccca agcatgccgc gacggcccat tacggtcatc cgtgagatca cgtattcccg
     3361 ggtcgctccg cgatgaagcg cgccaattgc gcttccgcgg tcgcgtccag gtgtccccac
     3421 ggctcccgca tcgccttcgt ctggattacg cgccggacca gtagctcggt tttcgtcgcc
     3481 cgcaccgaga agtcgatcga ttgcatcgcc cagaggacat agggcagctc ggactggaag
     3541 agcgcctcgg cccccgcccg gtcgccgcgc agcaggagct ggccgaccgg ccccgacgct
     3601 ccaccggtca ggcagatcag ccccgcctcg tgcgccgcca gcatgt
//
LOCUS       DASSGX010000623         3447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1196946, whole genome shotgun sequence.
ACCESSION   DASSGX010000623 DASSGX010000000
VERSION     DASSGX010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1196946"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="VFI12_11280"
     CDS             complement(<1..776)
                     /locus_tag="VFI12_11280"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET7057038.1"
                     /translation="MARYVLGRLISLIFVLLVVSFIAFVLMHQVPGGPFDTGERRLPE
                     ATRQAQLHKYGLDKSLPEQYVRYIWAVLHGDFGVPFQSPSESVIGLIARAWPVTIKIG
                     VPTILIAFTLGTLLGFIAALRQNSWIDYLVTTVATLGLTIPSFVVATWLILLLSIQLH
                     WLPTGGWGQPKHYIMPVIAYSLAPMALVARYVRVSILEALHADHVRTARAKGLSERRV
                     LRLHVARNALIPATTVLLPEIPNILTGSIFIEQMFRIPGLG"
     gene            1089..1742
                     /locus_tag="VFI12_11285"
     CDS             1089..1742
                     /locus_tag="VFI12_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HET7057039.1"
                     /translation="MNIPDVNALVPMVVESTNRGERAFDIYSRLLKDRIIFLGTPVDD
                     QIANLIIAQMLFLEHEDPNQDIRLYINSPGGVVYSGLAIYDTMQMIKPDVATFCMGMG
                     ASMAAVLLAGGAKGKRFALPNARVMIHQGSSGFRGAVPDIEVAARETLSLTNKLTEIL
                     ALHANQPFDKVKHDTERDYYMTAADAKEYGIIDEVLEPQKLADLAIPAGSEPNGKKK"
     gene            1896..3137
                     /gene="clpX"
                     /locus_tag="VFI12_11290"
     CDS             1896..3137
                     /gene="clpX"
                     /locus_tag="VFI12_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HET7057040.1"
                     /translation="MHYRCSFCNKGQEEVSRLIAGPNQVYICNECVQLCREIIEEEEP
                     QVPRPAVSTPKISTPRDLYEQLNQYVVGQDRAKRILSVAVYNHYKRINAGLESDDDIE
                     LQKSNILLVGPTGCGKTLLAQTLAKLLDVPFSIADATALTEAGYVGEDVENILLKLIQ
                     NAGDVTRAQTGIIYIDEIDKISRKSDNPSITRDVSGEGVQQALLKIIEGTVANVPPQS
                     GRKHPHQEYIQIDTRNILFICGGAFEGLDEIVGRRIGKHAPIGFGGAGEQAAETNPLQ
                     YLSHDDLLKYGLIPEFVGRLPVTVALDPLSRDELKRILVEPKNAVVKQYQKFFALDSV
                     ELTFTDEALEATARQAEERKTGARGLRTTIEEVLLDVMYEIPSMENVRKCVVDENVIV
                     NRSRPLLMTETEKQIPYAESA"
BASE COUNT          681 a         1115 c         1099 g          552 t
ORIGIN      
        1 cccaagcccg gaatccgaaa catctgctcg atgaagatcg agccggtcag gatgttcgga
       61 atctccggca gcagcacggt cgtcgccggg atcagcgcgt tgcgggctac gtgcagccgc
      121 aagacccgtc gctccgacaa ccccttggcc ctggcggtgc ggacgtggtc ggcgtggagc
      181 gcttcgagga tagagacgcg cacgtaccgg gcgaccaggg ccatcggggc cagcgaatag
      241 gcgatcaccg gcatgatgta gtgcttcggc tggccccagc cgccggtcgg taaccagtgg
      301 agctggatgc tgagcagcag gatcagccag gttgcgacga cgaagctcgg gatcgtcagg
      361 cccagcgtcg cgacggtcgt cacgaggtag tcgatccacg agttctggcg gagcgcggca
      421 atgaagccga gcagcgtgcc gagggtgaag gcgatcagga tcgtcggcac cccgatcttg
      481 atcgtgaccg gccatgcccg cgcgatcaag ccgatgaccg attcggacgg gctttggaac
      541 ggcacgccga agtcgccatg gagcacggcc cagatgtacc gcacgtactg ctcggggagc
      601 gacttgtcga gaccgtactt gtggagctgc gcctgtcggg tcgcctcggg caagcggcgc
      661 tcgccggtgt caaacggacc gcccgggacc tggtgcatca ggacgaatgc gatgaaggag
      721 acgacgagca gtacgaagat gagactgatc aggcgtccaa ggacataccg cgccaccgcg
      781 ctgcctcttc cgtatccggg ttgaacgtag cgctgagtct agcacgcggc atcggcgggg
      841 tcccggcaag gcgattggtc cccttcaggc ggatcatcct gccgggattc gggatgaacg
      901 tacaccccat ttgtcctatc cttgctcgac acgacggagt atgattgagt cctgaggcgc
      961 tcgcggctga gcgacaatca cgagtctcgc ggatctcccg cctgctgccc tggtcacgag
     1021 gcagagagca gaccaatacc gggcgcgcag gttgccgaca atcgccgcca gacgaggaca
     1081 ccgaatatat gaacatcccc gacgtcaacg cactcgtccc gatggtggtc gaaagcacca
     1141 atcgcggcga gcgcgccttc gatatctatt cccggttgct caaggatcgg atcatcttcc
     1201 tcggcacgcc ggtcgatgac cagatcgcga acctgatcat cgcccagatg ctcttcctcg
     1261 agcatgagga tcccaaccag gacatccgcc tctacatcaa ctcgccgggc ggcgtcgtct
     1321 actccggtct cgccatttac gacaccatgc agatgatcaa gccggacgtg gcgaccttct
     1381 gcatgggcat gggcgcgagc atggcggcgg tgttgctggc cggcggcgcg aagggcaagc
     1441 gcttcgcctt gccgaacgcc cgcgtcatga tccaccaggg ctcctccggc ttccggggcg
     1501 ccgtgcccga catcgaggtg gcggcgcgcg agaccctgag cctgaccaac aagctgaccg
     1561 agatcctggc cctgcacgcc aaccaaccgt ttgacaaggt caagcacgac accgagcgcg
     1621 actactacat gaccgccgcc gacgcgaagg agtacggcat catcgacgag gtgctggagc
     1681 cgcagaagct ggccgacctc gcgatcccgg ccggatccga gccgaacggg aagaagaagt
     1741 agcgggagca tttcccgatc cggatgggga cgttgcggct atcaggcgtg tccccattcc
     1801 cgaccccggt aacgtgtaat tcgtaaccgg cagtgctgat agcgagcggc tgacgcgacc
     1861 cggtcctcag ggcctatcta caccgaggtg agctggtgca ctatcgatgt tcgttctgca
     1921 ataagggcca ggaagaagtt agccggctga ttgcgggacc gaatcaggtc tacatctgta
     1981 acgagtgcgt tcagctctgc cgcgagatca tcgaggaaga ggagccgcag gttccgcggc
     2041 cggccgtttc cacccccaag atctcgactc cgcgcgacct gtacgagcag ctcaaccagt
     2101 acgtcgtcgg gcaggaccgc gccaagcgaa tcctctcggt cgccgtctac aaccactaca
     2161 agcgaatcaa cgccggtctt gagagcgacg acgatatcga gctccagaag agcaacatcc
     2221 tgctcgtcgg cccgaccggc tgcggcaaga cccttctggc ccagaccttg gccaagctgc
     2281 tcgatgtccc cttctcgatc gccgatgcca ccgccctgac cgaggccggc tatgtcggcg
     2341 aggatgtcga gaacatcctg ctcaagctga tccagaacgc cggcgatgtc acccgggccc
     2401 agaccggcat catctacatc gacgagatcg acaagatctc gcgcaagtcg gacaacccgt
     2461 cgattacccg ggacgtctcg ggcgagggtg tccagcaggc gctgctcaag atcatcgagg
     2521 gcaccgtcgc caacgtgccg ccccagagcg gccgcaagca cccgcaccag gaatacatcc
     2581 agatcgacac gcgcaatatc cttttcatct gcggcggcgc gttcgagggg ctggacgaga
     2641 tcgtcggccg gcgaatcggc aagcacgcgc cgatcggctt cggcggcgcg ggcgagcagg
     2701 ccgcggagac gaatccgctc cagtacctca gccacgacga tttgctcaag tacgggctga
     2761 ttcccgagtt cgtcggccgc ctgccggtga ccgtcgcgct cgacccgctc agccgggacg
     2821 agctcaagcg gatcctggtc gagccgaaga atgccgtcgt caagcagtat cagaagttct
     2881 tcgcgctcga tagtgtcgaa ctgaccttta ccgacgaggc gctcgaagcg accgcccgcc
     2941 aggctgagga gcgcaagact ggcgcccgcg gcctgcggac cacgatcgag gaagtgctcc
     3001 tcgacgtgat gtacgagatc ccgtcgatgg agaacgtgcg caagtgcgtc gtcgacgaga
     3061 acgtgatcgt caaccgcagt cgcccgctcc tgatgacgga gacagagaag cagatcccct
     3121 acgcggagtc ggcgtaaccg aggcgcgaag tcgggaaggc gcgaaggcgc ggagcaattg
     3181 atctacggtc gggcgaattt cgcccggccg tttctcgtta catcgctgtc gtcccaatgc
     3241 ccgggcctga agacccgggc tgacatctga acccggctaa cgctggctgg ggatgcgcgc
     3301 ccccaacgtg gcgccggtgc ctccgtgccc cgcgatggcc ggaacggcca tgaccgtagc
     3361 gcggtccccc cgtgggccgc ccggacccgg tcctgacccg gtcgttggtg gagaccaatc
     3421 ccggtgccgg gccggtgcat gcaagca
//
LOCUS       DASSGX010000624         5327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_323838, whole genome shotgun sequence.
ACCESSION   DASSGX010000624 DASSGX010000000
VERSION     DASSGX010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323838"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VFI12_11295"
     CDS             complement(<1..559)
                     /locus_tag="VFI12_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7057041.1"
                     /translation="MSSLIERCHGDVVDVSFSRRSVLRAAGSLALAGVVSRRMSLSVL
                     AQDATPAAGNYPVVEVTAENYKFTLPESIAGGWTELTFHNKGPADHHAMFMKLNDGTS
                     FDQFKQAADAATDLGALFAKSVCIGGAPSISAGETASTILNIEPGNYAVICVIPDETG
                     KPHYLMGMMNSLTVTEATSELAAPQA"
     gene            836..1141
                     /locus_tag="VFI12_11300"
     CDS             836..1141
                     /locus_tag="VFI12_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7057042.1"
                     /translation="MQRWEYLLVACVPRGGTMRPKFQNGEVIPGWESSTVPGYCNACG
                     NEDWELVSAVNDPSSERASVIDLIFKRPVELGAHSDILRKMPPGSSGSNQAEESRSA"
     gene            complement(1159..3828)
                     /gene="alaS"
                     /locus_tag="VFI12_11305"
     CDS             complement(1159..3828)
                     /gene="alaS"
                     /locus_tag="VFI12_11305"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HET7057043.1"
                     /translation="MSSDAQSRPSHGHRYMTGAEVRAAFVSFFESKGHREVPSSSLVP
                     INDPTVLLTTAGMQQMTPYFLGLETPPHNRLCSVQKCFRTVDIEEVGDESHCTFFFML
                     GNFSIGDYFKRESLAWSWEFLTEVLGLPAERLYPTVHPDDTDAYAIWRDEIGVPEERI
                     GKLADNWWGPVGNTGPNGPDSEIYFDLGPEAGDTGEGPGDGSRYLEVWNNVFMEFFQA
                     PDGSRTPLPKQHVDTGMGLERLTMIMQGAKSIYDIDLYQAIIGKVASEVGVTYGMDEK
                     VDRSLRVVADHIRGGTFLIADGVLPGNEGRSYVLRRILRRAIKNGRQLGIDKPFLSDL
                     AEVVIAQFADNYPELRERRSQIIKVLNHEEQTFRRTLSTGMGRFQALADSISATGGTV
                     VPGDEVFRLYDTYGFPADLTRDLAEDAGLSIDSAGFETAMQAQREASRAGGAFKDTAR
                     HRAEMYVHAAGQKTEFLGYDTLTSDATIVALIGPGGVIDVADAGETVEVVLDRTPFYG
                     ESGGQIGDTGRIRTDTGLIDIDDTVKPTPDLFVHRGVVAEGFIRAGETVTATVDGSRR
                     RAVQRNHTATHLLHKALRMVLGDETHQAGSLVAPDRLRFDFTSLESMASTQVERVAEI
                     VNAEILEDRPVDWRQMPMNEAVAEGAMALFGEKYGDVVRVVDIEGFSKELCGGTHVGH
                     TGEIGPFLIVSEGSVAAGVRRIEALTGSAATNRMLAQQRLLDRVSRDLRTTWHEVPEA
                     IALLQERTRATEREMAALRGKLTGSLVDELLHSASDVAGVPMIAQRVAVGERGDLRQL
                     ADRLRDRQQSGVVVLGAVFDDKPALLAMVSPDLVSRGLKAGDIIREIAQYVEGRGGGR
                     PDLAEAGGKNAAGLDEALLAVSGAIEAALEPST"
     gene            4025..4822
                     /locus_tag="VFI12_11310"
     CDS             4025..4822
                     /locus_tag="VFI12_11310"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HET7057044.1"
                     /translation="MAGQGNSEVRWQKLRRDEIAALAVRDPVVLLPIGAIEQHGPHLP
                     VDTDTNSVSTIAERAAHQVGPDVALVLPPIHWGLSPYWMPFAGTITLRPETILALISD
                     IGCSVSAHGFRRMVIINGHGGNGGIIGVAATQLADHGIRAVALSYWALLGSELSSITP
                     ADHGHIGHAGQTETSIQLHLQPERVDPVYSSFTSWADLSTMADDLLTPGAYKPPLPLV
                     ESPTGVYGDLTQAQAELGELIVDLVTDRLADLIRAFPLSQPGGETQG"
     gene            5028..>5327
                     /locus_tag="VFI12_11315"
     CDS             5028..>5327
                     /locus_tag="VFI12_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7057045.1"
                     /translation="MRALGLSRLLAVAIIATLALSVLGAVSPAKAELARGSVMIHSRV
                     CPLNLPAGSMLFDDCHSHPGPSGAEFTVDNRAPKAIGANGNVSFGRVTAGDHLVTL"
BASE COUNT          949 a         1756 c         1655 g          967 t
ORIGIN      
        1 ccgcctgcgg cgccgcgagc tcgctcgtcg cctcggtgac ggtcagggaa ttcatcatcc
       61 ccatcagata gtgcggcttg ccggtttcgt ccgggatcac acagatgacc gcgtagttac
      121 cgggttcgat attgaggatg gtcgacgccg tttcacctgc agagatcgat ggggcgccgc
      181 cgatgcagac gctcttcgcg aacagcgcgc cgagatcggt cgcggcgtct gcggcttgct
      241 tgaactggtc gaacgacgtg ccgtcgttca gcttcatgaa catggcgtgg tgatcagccg
      301 gacccttgtt atggaaggtg agctcggtcc agccgccggc aatgctctcc gggagcgtga
      361 acttgtagtt ctccgccgtt acctcgacga ccggataatt cccggcggcg ggtgttgcgt
      421 cctgcgcgag cacggacaac gacatccgcc gcgagaccac gccggcgagc gcgagagagc
      481 ccgcggcgcg gagcacgctc cggcgcgaga acgaaacgtc aacaacgtca ccatgacagc
      541 gctcaattag cgaagacacg acagctccac tccttttcga caaagtacga ccttgcgcgc
      601 cgacgattcg gcgtgcctgc gcgcccgccc gggatcggcg gcccatgtcg tcgtctcagg
      661 aagggatatc gattaacgat acgactaata cttaaattcg gttcttctca atcctgttcg
      721 acgcgacaag ggtgcgctgg gcactcccac acgacgtgcc cggccaaggc aagggatatc
      781 tcgcgccaca gatgccgcat atattgttcg gacgggcttc cgaaaggggg aggcgatgca
      841 gcgatgggag tacctgctgg ttgcgtgcgt tccgcgcggg ggaaccatgc gcccgaagtt
      901 ccagaatggc gaggtcatcc ccggctggga aagctccact gtgcccggct actgcaacgc
      961 ctgcgggaac gaggactggg agctggtcag cgcggtcaac gatccgagct ccgaaagggc
     1021 gagcgtcatc gacctcatct tcaagcgccc cgtcgaactc ggcgcccaca gcgatatcct
     1081 ccggaagatg ccccccggct cttcggggtc aaaccaggcc gaagaatccc gcagtgcctg
     1141 acccggcccg aaatgcgttt acgtcgatgg ctccagcgct gcctctattg cgccgctcac
     1201 tgccagtagc gcttcgtcaa gcccggccgc gttcttgcca ccggcctcgg ccagatccgg
     1261 ccggccgcca ccgcgcccct cgacgtactg cgcaatctcg cggatgatgt cgcccgcttt
     1321 caacccacgc gacacgaggt ccggtgagac catcgcaagc agagccggct tgtcgtcaaa
     1381 gacggcgccg agcacgacga cgccgctctg ctgccggtcc cggagccggt ccgcgagctg
     1441 gcgcagatcg ccgcgctccc cgacagccac ccgctgcgcg atcattggca cgccggcgac
     1501 gtcggacgcg gaatgaagca gctcgtccac cagcgaacca gtcagtttgc cgcgcaaggc
     1561 cgccatctcg cgctcggtcg cccgggtccg ctcctgcagc agcgcgatcg cctctggcac
     1621 ctcgtgccag gtcgtccgca gatcccgcga aacgcgatcc agcagccgct gctgcgccag
     1681 catccggttg gtcgcggcag agccggtcag cgcctcgatc cggcgcactc ccgccgccac
     1741 gctgccctct gagacgatca agaacggtcc gatctccccg gtgtggccga cgtgagtgcc
     1801 gccgcagagt tccttcgaga agccctcgat gtccacgacc cgcacgacat cgccgtactt
     1861 ctcgccgaag agcgccattg ccccctcggc gaccgcctcg ttcatcggca tctgtcgcca
     1921 gtccaccggg cgatcctcaa ggatctccgc gttgacgatc tcggcgaccc gctcgacctg
     1981 ggtcgacgcc atcgattcga ggctcgtgaa atcgaagcgc aaccggtccg gcgcaacgag
     2041 cgagcccgcc tgatgggtct cgtcgccgag caccatcctg agcgccttgt gcagcagatg
     2101 cgtggcggtg tgattgcgtt gtaccgcgcg acgacgcgag ccatccaccg tggccgttac
     2161 cgtctcgccg gcgcggatga agccctcagc gaccacgccc cggtgcacga aaagatccgg
     2221 cgtcggtttg acggtgtcgt caatgtcgat caggccggta tcggtccgga tgcggcccgt
     2281 atcgccgatc tggccgcccg attcgccgta gaacggcgtc cgatcgagca cgacttcgac
     2341 cgtttcgccg gcgtccgcaa cgtcgatgac gcctccgggt ccgatcaggg cgacgatcgt
     2401 cgcatcggac gtcaacgtgt catacccaag gaactcagtc ttctgcccgg ccgcgtggac
     2461 atacatctcg gcccggtgcc gcgcggtgtc cttgaaggcg cccccggccc gactcgcctc
     2521 acgttgcgcc tgcatcgccg tttcgaaccc tgccgaatcg atacttagac cggcgtcctc
     2581 cgcgaggtcg cgcgtcaggt ctgccgggaa cccgtatgtg tcgtagaggc ggaacacctc
     2641 gtcgccgggc acgacggtgc cacccgtcgc ggagatcgag tcggccaacg cctggaagcg
     2701 gcccattccg gtgctgagcg tgcggcggaa ggtctgctct tcatggttga gcaccttgat
     2761 gatctggctg cgacgctcgc ggagctccgg ataattgtcg gcgaactgcg cgatcacgac
     2821 ctcagcgagg tcagacagga acggcttgtc gataccgagt tgtcggccgt tcttgattgc
     2881 ccgccgcagg attcggcgca ggacatacga ccgcccctcg ttacccggca agacaccgtc
     2941 ggcgatcagg aacgtgccac ctctgatgtg gtccgcaaca acacgcagcg agcggtcgac
     3001 cttttcgtcc atgccatagg tgacaccgac ctcgctggcc accttgccga tgatcgcctg
     3061 gtagaggtcg atgtcgtaga tcgacttcgc cccttgcatg atcatcgtca atcgctcaag
     3121 gcccatgccg gtgtcgacgt gctgcttcgg gaggggagtg cgtgaaccat ccggcgcctg
     3181 gaagaactcc atgaagacgt tgttccagac ttcgaggtag cggctgccgt cccccggccc
     3241 ctcgcctgtg tcgccggcct ccggaccgag atcaaagtag atctccgagt ccggtccgtt
     3301 cggtccggtg tttccgaccg ggccccacca gttatcggca agcttgccga tccgttcctc
     3361 gggcacgccg atctcgtcgc gccatatcgc gtaggcgtcg gtatcgtccg ggtgcacggt
     3421 cggataaagc cgctcggccg gcaaccccaa gacctcggtc aggaactccc acgaccaggc
     3481 cagcgactcc cgcttgaaat aatcgccgat cgagaaattg ccgagcatga aaaagaaggt
     3541 gcagtgggac tcgtccccga cttcctcgat atcgaccgtg cggaaacact tctggaccga
     3601 gcagagtcgg ttatgcggcg gcgtctccaa tccgaggaag tagggcgtca tctgctgcat
     3661 gccggccgtg gtcaggagca cggtcggatc gttgatcgga accagcgacg acgacggcac
     3721 ctcacgatgg cccttcgact cgaagaagga gacgaatgcg gcgcgcactt ctgccccggt
     3781 catgtagcgg tgaccgtgcg agggacgtga ttgcgcgtct gagctcatct ccgatgttcc
     3841 ttctggcgtt gccgaatgca ggcggcgcgg aaaatgacaa gacgcggccg aatgccctgc
     3901 cgcgtcgcga atgtcaagta tagaggaggc gagaagtcgg agagtcgagc agtcgagaag
     3961 caggaagacg cgcgggacga taccgtaccg gaaacaggcg ataacgagat agatggagtg
     4021 gggtatggcg gggcagggga attccgaggt tcggtggcag aagttgcggc gggatgagat
     4081 cgcggcgctg gcggtacggg atcctgtcgt gttgctgcca atcggcgcga tcgagcagca
     4141 cgggccacat ctgccggtcg acactgacac caactcggtc agcacgatcg cggaacgcgc
     4201 agcacaccag gttggaccgg acgtcgccct tgtgttgccc ccgatccatt ggggcctctc
     4261 gccgtactgg atgcccttcg ccgggacgat cacgttgcgg ccggagacga tcctggcact
     4321 gatctcggac atcggttgca gcgtctctgc ccatggattt cgccggatgg tcatcatcaa
     4381 cgggcacggc ggcaacggcg gcatcatcgg cgtcgccgcc actcagttgg ccgaccacgg
     4441 catccgcgct gtcgcgcttt cctactgggc gctgctcggc tccgagctca gctcgatcac
     4501 gccggccgat cacggccaca tcggccacgc cggtcagacc gaaacctcga tccagctcca
     4561 ccttcaaccc gagcgggtcg acccagtcta ttcctcgttt accagctggg cggacctgag
     4621 cactatggca gatgacttgc tgacgcctgg ggcgtacaaa ccacccctgc cgttggtcga
     4681 atcgccgacc ggtgtctacg gcgatctcac gcaagctcag gccgaactgg gcgaactcat
     4741 cgtcgatctc gtcaccgacc ggctggccga tctcatccgc gcattcccgc tgtcgcaacc
     4801 cggtggtgag acgcagggat aaccaatcga aggtctcctt cgaaccggag atttctggcg
     4861 gagcgatagc gataagcgag gttgaaacat cgacccgaac gatgtgaaac ctttcgccac
     4921 gccctgtcgt tatgaggctg aacatccgaa ttggctggat gtcggatgcg gcgtttccgt
     4981 gtggaggcgt tgtgttgggt gtcgttcatt caatgaggag ttcagtcatg cgcgcgttgg
     5041 gtctttcccg tctgcttgcc gtcgcgatca tcgccacgct tgccctgtca gtcctgggtg
     5101 cggtgtcacc cgcaaaagct gagttggctc gtggttccgt gatgatccat tcgcgggtct
     5161 gtccgctcaa cctgccggct gggtccatgc ttttcgacga ctgtcactcg catcccggcc
     5221 cgagcggcgc cgagttcacg gttgataacc gcgctcccaa ggcgatcggc gcgaacggca
     5281 acgtcagctt tggccgcgtg accgccggcg atcacctggt caccctg
//
LOCUS       DASSGX010000625         3433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_215646, whole genome shotgun sequence.
ACCESSION   DASSGX010000625 DASSGX010000000
VERSION     DASSGX010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215646"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VFI12_11320"
     CDS             complement(<1..302)
                     /locus_tag="VFI12_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7057046.1"
                     /translation="MKFRKPTDNSNSMIMLGGWLFADLLLGLAMLFLTSGTSGSPPPT
                     ATPTSTPNQLATAEVRAARTATLQAENVAALNATVESASDAAQATEAARATADARLS"
     gene            complement(332..2056)
                     /locus_tag="VFI12_11325"
     CDS             complement(332..2056)
                     /locus_tag="VFI12_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7057047.1"
                     /translation="MVQIRARKSNQPFPRAQGDNFPANDAMDDSELSTAPLPRSRPRP
                     ATTRDLADDINGLAASLEEIDTRSTERLRDLASSLSTEEGRLRWADVDLRRAFNSEYL
                     AHFYAVRREGGYAPTTVAVADRIRNVLVLLPIALTWFALYESTKAYREYIDAHPDEIS
                     QPFLLLWQRGFDGNSGFFATSFSTLAIIDAMIIGVIIALTFYSHGRRERREESIERTA
                     QKFQADVDNILAEATVALAPDRAGRPAMLARSVERLAERFEHNSQELIARLRAEHDRL
                     VSIASRREKEFADFGLFASGMRAGAEETHRLLIELRQVSTALQHALEDMTSEVGVTGD
                     QNKSLLSAVTALERMVATGIQSDHGVTRRLAEASEVLAEAAERSLAGAEAAAQAGRVA
                     TEAVRGIAEIGASLASGQNRVEHAIANESEANTRLAEALRSSVTGVSSTTRTLAEIQA
                     SLVGIRDEFRDIARSNQEHASSLSRMLTEQTTISSGIAKIARDIGTTGASNQQQQQDM
                     NEELSSLIKRLDNITPTPAPQPGTGGFETVQTNEGVRRVLTNAPDFLSDPVESPGGRD
                     RGNRGRRD"
     gene            2324..>3433
                     /locus_tag="VFI12_11330"
     CDS             2324..>3433
                     /locus_tag="VFI12_11330"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET7057048.1"
                     /translation="MVGREAVVYQLRQATGKVAALRCWLEDDISPEMIARYRALGSPG
                     TLRRLRGIERSPIIGSLGLHADGVRVEADDFRSETRPVVVLDWLMGPTVLAAVDRACR
                     SKDTGSLSALAQAWRVAVAAATEVGFVHGDLTADNAIVRPKEGIAFVDYDTAYWPDAP
                     SVPVLDPAVAYRHPRGATTRPEYADDFAALLIYTSLRILAVWPELRTEHGQPATMRGA
                     GLLFQARDLAQPDGSPLFGKLRVVNDAQVQGLVGILREACLSDPDNMPTFAESLTLAA
                     NVASAPVIQPPHGPRSGRVSSLLSSTVSPVREPAGRRIDRPREAPIAPGVEELWPERQ
                     RNWRPERFGGLLAALEDRDVNQAEALWAHLRDEPGA"
BASE COUNT          532 a         1118 c         1168 g          615 t
ORIGIN      
        1 gagaggcggg catccgcggt ggcgcgggcc gcctcggtgg cttgcgcggc atcggacgcg
       61 ctctcgaccg tagcattcaa ggccgcgacg ttttcagcct gcaatgttgc cgtgcgcgca
      121 gcgcgaacct ccgccgtcgc cagttggttc ggcgtcgagg ttggagttgc ggtcggtggc
      181 ggactgccgg aggtgcccga ggtgaggaag agcatcgcca agcccagcag caggtcggca
      241 aagagccagc cgccgagcat gatcatgctg ttgctattat cggtcggttt ccggaatttc
      301 acccgaagaa ctccagtagt ccggcgctcg gttagtcgcg ccggccgcga ttaccgcgat
      361 cacgtccacc ggggctctcg acggggtcgc tcaggaagtc gggcgcattg gtcaggaccc
      421 ggcgcacgcc ttcgttggtc tgcacggtct cgaagccacc ggtccctggc tgtggcgcgg
      481 gcgtgggggt gatgttgtcc aggcgcttga tcaggctcga cagctcctcg ttcatatctt
      541 gctgctgttg ctgattgctc gcgccggtcg tgccgatgtc gcgggcgatc ttcgcgatac
      601 ccgacgagat cgtcgtctgc tcggtcaaca tgcgtgagag cgacgacgcg tgctcctgat
      661 tggagcgcgc gatatcgcgg aattcgtccc ggatgccaac caggctggcc tggatttcgg
      721 caagcgtgcg cgtggtcgag gagacgccgg tcacgctcga gcgcagcgcc tcggcgagcc
      781 gcgtgttggc ctcgctctcg ttggcaatcg cgtgctcgac ccggttctgg cccgatgcga
      841 ggctcgcgcc gatctcggca atgccgcgaa ccgcttcggt cgccacccgg ccggcctgag
      901 cggctgcttc agcaccggcc agggagcgtt cggccgcctc ggcaagcacc tcggacgcct
      961 cagccaggcg gcgcgtcaca ccgtgatcgc tctggatccc ggtcgcaacc atccgttcca
     1021 gggccgtgac ggccgaaagc agggacttgt tctggtcgcc ggtcacgccg acctcgctgg
     1081 tcatatcctc gagcgcatgc tgaagggcgg tcgagacctg gcgcaactcg atcagcaggc
     1141 ggtgtgtctc ctcagcgccc gcacgcatgc cactggcgaa gagtccgaag tccgcgaatt
     1201 ccttctcgcg tcggctggcg atggagacga gacgatcgtg ctccgcgcgc agccgggcaa
     1261 tcagctcctg gctgttgtgc tcgaaccgct cggccaatcg ctcgacgctg cgagcgagca
     1321 tggccggccg gccggcgcga tccggggcaa gggccacggt cgcttcggca aggatgttgt
     1381 cgacgtccgc ctggaacttc tgggcggtcc gctcgatcga ctcctcgcgg cgctcgcggc
     1441 ggccgtgcga atagaaggtc aacgcgatga tgacgccgat gatcatggcg tcgataatgg
     1501 cgagcgttga gaaggaggtc gcaaagaagc cgctattgcc gtcgaatccc cgctgccaga
     1561 gcaggaggaa cggctggctg atctcgtccg gatgcgcgtc gatgtactcg cgatacgcct
     1621 tggtcgactc gtagagcgcg aaccatgtca gcgcgatcgg caaaagcacg agcacgttcc
     1681 ggatccggtc cgcaaccgca accgtcgtcg gcgcgtagcc accttcgcgg cggaccgcgt
     1741 agaagtgcgc aaggtattca ctgttgaacg cccgccgcag atcgacatct gcccagcgca
     1801 ggcggccttc ctctgtgctc agggacgagg cgagatcgcg cagacgctcg gtgctgcgcg
     1861 tgtcgatctc ctcgagcgac gccgccagac cgttgatgtc gtcggcgaga tcacgcgtgg
     1921 tggccggccg tggccgcgag cgcggcaagg gcgccgtgct cagctcactg tcgtccatcg
     1981 cgtcgttcgc gggaaaattg tcgccctgtg cgcgggggaa gggctggttg ctcttgcgag
     2041 cccgaatctg gaccatacgc ctcccgaatg atcagcgaac gtcgtctcct ctaatgtgga
     2101 cctttgacgc gtgtcgtctg atctgaagct cactgtacca ttgcaaaagc gtgtcccgta
     2161 acccgtacag gccggatgct gaagagaatc gggactatct gcacccatga ctctacagac
     2221 gctttacgac aacgcaatgt tacgcggtcc ccggtcagtc cctgttattg ggcagttggg
     2281 cggggggacg atcgcgacca tttcggacag ccagcctttc cgcgtggtcg gccgggaggc
     2341 ggttgtctac cagctccggc aggccaccgg caaagtcgcg gcactgcgct gctggctcga
     2401 ggacgatatc tcgcccgaaa tgatcgcccg gtaccgcgcg cttggcagcc cgggcacatt
     2461 gcggcgcctg cgcggcatcg agcgctcgcc cattatcggc tcgctcgggc tccatgccga
     2521 cggcgttcgc gttgaggccg atgacttccg atccgaaacc cggccggtcg tcgtgctgga
     2581 ctggctgatg ggcccgaccg tgctggcggc ggtggatcgc gcctgccgct cgaaggacac
     2641 ggggtccctg tcggcgctgg cgcaggcttg gcgggttgcc gtcgcggcgg ctaccgaggt
     2701 tgggttcgtc cacggtgatc tgacggccga caacgcgatc gtccggccga aagagggcat
     2761 tgcgttcgtt gattacgaca ccgcctactg gccggacgcg ccgtccgtcc cagtactcga
     2821 cccggcggtg gcctaccgcc acccccgggg ggcgacgacg cgtcccgagt acgccgatga
     2881 tttcgctgca ttgctgatct acacgtccct ccggatcctg gctgtctggc ccgagttgcg
     2941 gaccgagcac ggccagccgg ccaccatgcg tggcgcgggg ctgctcttcc aggcgcgcga
     3001 tctcgcccag ccggacggat cgccgctctt tggcaagctg cgggttgtca acgatgcgca
     3061 ggtgcaggga ctggtcggta tcctgcgcga ggcgtgcctc tcggaccccg acaacatgcc
     3121 gacctttgcc gaatcgctga ccctggcggc aaacgtcgcc agtgcgccag tgattcagcc
     3181 tccgcacgga ccacggagcg ggcgagtttc atccttgctc tcgagcacgg tctcgcctgt
     3241 ccgggagcca gctggccggc gcatagaccg accccgggag gcgccgatcg cacccggagt
     3301 cgaagagctt tggccagagc gccagcggaa ttggcggccc gagcggtttg gcggcttgct
     3361 cgcagcactg gaggatcgcg acgtgaacca ggcggaagcg ctctgggcgc atctccgcga
     3421 cgagcccggg gca
//
LOCUS       DASSGX010000626         2546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_760988, whole genome shotgun sequence.
ACCESSION   DASSGX010000626 DASSGX010000000
VERSION     DASSGX010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760988"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1526
                     /locus_tag="VFI12_11335"
     CDS             <1..1526
                     /locus_tag="VFI12_11335"
                     /inference="COORDINATES: protein motif:HMM:NF016859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7057049.1"
                     /translation="KCYGRDLSTGKLVELGLAVGVIAAQSIGEPGTQLTMRTFHIGGT
                     ASRVSEQSTLDAKHAGTVRYQGLQVVETKGEDNANAGQRVVMNRTGSVVVQDAKGRDR
                     ERYPIVYGARLKVADGQQVEVGQILVEWDPYTFSILTEDAGTVKYKDLISDVTYHEEV
                     DEVTGLSRKIVVDSPDEKKQPTMEIRDKSGKVSRKYHMPSHAHLMVEDGEAVFAGEVL
                     AKIPRETTKTKDITGGLPRVVELFEARKPRETAIISEIDGLVRHGGIVKGQRKIIIVP
                     EDGGEPREYSLPRGVHVNVQEGDRVRAGEPLMDGPSNPHDILSVLGEKALHAYLVNEI
                     QEVYRLQGVNINDKHIEVIVRQMMRWVKIEDVGDTEFLIEEQVDRFRFMEENERVIAA
                     GGRPATGRPLLLGITKASLSTDSFISAASFQDTTRVLTEASLSGKVDHLRGLKENVTM
                     GRLIPAGTGFEYYRHVRIPADEPPPPPPPTPEELELEREMEYFVEPEEAFTGRDDIE"
     gene            1764..2324
                     /locus_tag="VFI12_11340"
     CDS             1764..2324
                     /locus_tag="VFI12_11340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7057050.1"
                     /translation="MNVLRNPDASDGTRYWDINGNATIEQVDGNPCFVVRRGGILTQT
                     VSISEGNESLYVVLIGRVWSEYVHPDGAITDRPYLYGMARDESKFILGYLQGGTMGGR
                     GRAQEWETVYGIFHVPDGTMRIHFQLGQGLRKGVPYSGAAARFDDLALYAVTDESAAR
                     AIVAGRVTNAPIRLGAVTLPVRRPSR"
BASE COUNT          507 a          748 c          862 g          429 t
ORIGIN      
        1 ccaagtgcta cggccgcgac ctctcgaccg gcaagctggt cgagctcggt ctggcggtcg
       61 gcgtcatcgc ggcgcagtcg atcggcgagc cgggcacgca gctcacgatg cgtacattcc
      121 acatcggggg aacggcatcg cgggtcagcg agcaatccac actcgacgcg aagcacgccg
      181 gcacggtccg ctaccagggc ctgcaggtgg tcgagacgaa gggggaggac aacgccaacg
      241 ccggccagcg tgtggtcatg aaccgcaccg ggtcagtcgt cgtacaggac gcgaaggggc
      301 gcgatcgcga gcgctatccg atcgtctacg gcgcgcggct gaaggtcgcc gacggccagc
      361 aggtcgaagt cggccagatc ctggtcgagt gggatccgta cacgttctcg atcctcaccg
      421 aggatgcggg cacggtgaag tacaaggatc tcatcagcga cgtcacgtat cacgaggagg
      481 tcgacgaggt caccggtttg tctcggaaga tcgtcgtcga ctcgccggac gagaagaagc
      541 agccgacgat ggagatccgc gacaagagcg ggaaggtgtc gcgcaagtac cacatgccgt
      601 cgcacgccca cctgatggtc gaggacggcg aggcggtatt cgccggcgaa gtgctcgcga
      661 agatcccgcg cgaaacgacc aagacgaagg acatcaccgg cggtctgccg cgcgtggtgg
      721 agctgttcga agcgcgcaag ccgcgcgaga cggcgatcat ctcggagatc gacggcctcg
      781 tccgccacgg cggcatcgtc aaggggcagc gcaagatcat catcgtcccg gaggacggcg
      841 gcgagccgag agagtactcg ctgccgcgcg gcgtgcacgt caacgtccag gaaggggacc
      901 gcgtccgcgc cggcgagccg ctgatggacg gcccgagcaa tccgcacgac atcctgtcgg
      961 tcctcggcga gaaggcgctg cacgcgtatc tcgtcaacga gatccaggag gtctaccggc
     1021 tgcagggcgt caacatcaac gacaagcaca tcgaggtcat cgtccggcag atgatgcggt
     1081 gggtgaagat cgaggatgtg ggcgatacgg aattcctgat cgaggaacag gtcgatcggt
     1141 tccggttcat ggaagagaac gagcgcgtga ttgccgcggg cggacgtccg gcgacgggcc
     1201 ggccgctgct gctcgggatc accaaggcgt cgctctcgac cgactcgttc atctcggcgg
     1261 cgtcgttcca ggacacgacg cgcgtgctca ccgaggcgtc actctccggc aaggtcgatc
     1321 atctgcgcgg cctgaaggag aacgtcacga tgggccggct gattccggcc ggcaccgggt
     1381 tcgagtacta ccggcacgtg cggatcccgg cggacgagcc gccgccgccg ccgccgccga
     1441 cgccggagga gctggagctg gagcgcgaga tggaatattt cgtcgagcct gaggaagcct
     1501 ttacgggaag agacgacatc gagtagaaga tcgggaattg ctgattggcg atttctgatt
     1561 gctgattgaa atggcgggct tgcctcgccg aagcgtcagc gaaggtgggc ggtggtcgaa
     1621 agatcactgc cctttttttg attgccggaa gctcgaagca cgagtggcgt ctgtgctgac
     1681 cctgcgttga cgattgaagt ttctctcctt cctcctcttt ctgacgcttg ccggggcgat
     1741 cccaaacggc cgacgtccac ccgatgaacg tgcttcgcaa tcctgatgcg agcgacggca
     1801 cccggtattg ggacataaat ggcaacgcca cgatcgagca ggtcgatggc aatccatgtt
     1861 ttgtggttcg acgaggcggc atccttacac agaccgtgtc catcagcgag ggtaacgaga
     1921 gcctgtacgt tgtgttgatt gggcgggtct ggtcggagta tgtgcatccc gatggggcga
     1981 tcaccgaccg gccatatctg tacggtatgg cgcgtgacga atccaaattt atcctcggct
     2041 acctgcaagg tggaacgatg ggagggcgcg gcagagcgca ggaatgggag acggtgtacg
     2101 gcatcttcca cgtccccgat ggtacgatgc gaatccactt ccagctcggg caggggctgc
     2161 ggaagggagt gccgtactcg ggggctgccg ctcgctttga tgaccttgcg ctctacgcgg
     2221 tgaccgacga gtcagcggcg cgcgcgatcg tcgcagggcg ggtgacgaat gctccaatca
     2281 ggctgggagc cgtaaccttg cctgttcgcc gcccttcgcg gtgagggtcg atggcaaccg
     2341 gagccggatg acgcacccac gctgccgatg gtagcatccg gacacatgcg gaaatccata
     2401 tcccgaaacc ggagaacgct caccatcgat cgagatctcg ccctcagcct tgtctcgcgc
     2461 ctacaacgag cagaagacat tcaatgagct gacccgtggg atgacgcagg caatgtatct
     2521 gcttccgatg caggacaact tcgatg
//
LOCUS       DASSGX010000627         2254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_652817, whole genome shotgun sequence.
ACCESSION   DASSGX010000627 DASSGX010000000
VERSION     DASSGX010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652817"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VFI12_11345"
     CDS             <1..400
                     /locus_tag="VFI12_11345"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HET7057051.1"
                     /translation="GTGAATPQGQKVIDYAMRFLGYPYVWATHGPDTFDCSGFTYYVT
                     IKTLKQDIGAGTWSQSISGRPVAFGDLRPGDLVFFQNTYTWGLSHVGIYIGDGKFIHA
                     ENEQTGVVISSLTSTYYKTRWYGARRITTD"
     gene            720..1886
                     /locus_tag="VFI12_11350"
     CDS             720..1886
                     /locus_tag="VFI12_11350"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET7057052.1"
                     /translation="MRHALQRQVDHGVTPGLVALVDHRGREHVETIGTMAFDSIAPME
                     RDAIFRLASMTKPITAVGAMVLVEECAIRLDDPVDEWLPELANRTVLRTINSPLDDTV
                     PANRPITVRDLFTYRCGYGEVAFEAPMCPMQAAMGERQLPLTEWLFPGTPDEFMRRLG
                     SLPLAHQPGERWLYHMSGEILGVLIARVTGKSLGAFLNERVFEPLGMKDTAFFVPEDK
                     IDRLPTCYGTDFPSANLIVLDEARGGKVSSPPVFEAGAGGLVSTVDDMLAFGRMLLNV
                     GTLGHERILSRPAVELMTMDHLTPGQKAASPFFPGFWATRGWGFGLSVVTARNDFAEV
                     PGRFGWDGAFGTSWYVDPKEELIGILMTQRRPDTLGIAPFIRDFWTSAYQLIDD"
     gene            1969..>2254
                     /locus_tag="VFI12_11355"
     CDS             1969..>2254
                     /locus_tag="VFI12_11355"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HET7057053.1"
                     /translation="MDAAQPIELTSKDSRFATSPTVYTIGHSTRPQSELIDLLRDFGV
                     RTLVDVRTVPRSRRNPQFNKDELTIALPAAGVKYAHVARLGGLRKGLGAES"
BASE COUNT          393 a          747 c          721 g          393 t
ORIGIN      
        1 gggcaccggc gcagccacgc cgcagggcca gaaggtgatc gactacgcca tgcgcttcct
       61 cggttacccg tacgtctggg cgacgcacgg accggacacc ttcgattgct ccggcttcac
      121 gtactacgtc acgatcaaga cgctcaagca ggacatcggc gcgggcacct ggagccagtc
      181 gatctccggc cggccagtcg cctttggcga tctccgtccc ggcgacctgg tcttcttcca
      241 gaacacctat acatggggtc tctcgcacgt cggcatctat atcggtgacg gcaagttcat
      301 ccatgccgag aacgagcaga ccggcgtcgt tatcagcagc ctgaccagca cctattacaa
      361 gactcgctgg tacggcgccc gccggatcac gacagactga ggcgagcaag cgagatctcg
      421 aacagtcgag cggaagggac gccaacgggc gtcccttctg cgcattgggt tcggttgaca
      481 agccgcatct ctgttgcgtt cctgcaatgc ggcgttgcca ggtgcggtcg cgcctgaggc
      541 tcccaacttg ccaatctcca ggtgtgtcac aaggggcgcg acgccgcgcc tttctgggca
      601 cgccccgcgc gaaggagaca cccaacatgg ttaccaccac atcccgaccc ggccttgacc
      661 gcgcataccc ggcgtccgcg gtactccccc gagctttcga ctcgggtcga ctggagcgga
      721 tgcgccacgc cctccagcgc caagtcgacc acggcgtgac gccggggctc gtcgccctgg
      781 tcgaccatcg cggccgcgag cacgtcgaga caattggcac gatggcattc gacagcattg
      841 cgccgatgga gcgggacgcc atcttccggc tcgcgtcgat gacgaagccg atcaccgcag
      901 tcggcgccat ggtcctcgtc gaggagtgcg cgatccggct cgatgatccg gtcgacgaat
      961 ggctgccgga gctggcgaac cgcacggtgc tgcggacgat caatagtccg ctggacgaca
     1021 cggtcccggc gaatcggccg ataacggttc gcgatctctt tacatatcgc tgtgggtatg
     1081 gcgaggtggc gttcgaggcg ccgatgtgcc cgatgcaggc ggcgatgggc gaacggcaac
     1141 tcccgctcac cgaatggctc ttcccgggca cgcccgacga gttcatgcgg cgcctgggca
     1201 gcctgcctct ggcgcaccag cccggcgagc gctggctgta ccacatgagc ggcgagatcc
     1261 tcggcgtact aatcgcgcgc gtgaccggca agtcgctggg cgcgttcctg aacgagcgcg
     1321 tcttcgagcc gcttggcatg aaggacacgg ctttcttcgt gccggaggac aagatcgacc
     1381 ggttgccgac ctgttatggg accgacttcc cgagcgccaa tctcatcgtg ctcgacgagg
     1441 cgcgcggagg caaggtctca agcccgcccg tcttcgaggc cggggcggga ggattggtct
     1501 cgaccgtgga tgacatgctc gccttcggcc ggatgctgct caatgtgggc accctcgggc
     1561 acgagcgaat cctgtcccgg ccggcggtcg agttgatgac gatggatcac ctgacaccgg
     1621 gccagaaggc ggcctcgccg ttcttccccg gtttctgggc gacgcgcggc tggggcttcg
     1681 gactctcggt cgtgacggcg cggaacgact tcgcggaagt acccggccgg ttcggctggg
     1741 atggcgcatt cggcacctcc tggtacgtcg acccgaagga ggagctaatc ggcatcctga
     1801 tgacgcagcg ccggccggat acgctcggca tcgcgccgtt catccgcgac ttctggacct
     1861 cggcctatca gttgattgac gactgacaaa cactcggctg ggccgcatcc ccatctgtgt
     1921 agcgtcacaa ggcattccga ctccccgccc gcctgctacg ctgaaggaat ggacgcggcc
     1981 cagccaatcg aactcacctc gaaagactca cgattcgcga cttcaccaac ggtttacacc
     2041 atcgggcatt cgacacggcc gcaatcggag ctgattgatc tgctccgcga tttcggggtg
     2101 cggacactgg tcgatgtccg gactgtgccg cgctctcgcc ggaatcccca gttcaacaag
     2161 gatgagctga cgatcgcgct gccggcggcc ggggtcaaat acgcgcatgt ggcacggctc
     2221 ggcggcctgc gcaaggggct cggggcggag tcac
//
LOCUS       DASSGX010000628         6467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_761132, whole genome shotgun sequence.
ACCESSION   DASSGX010000628 DASSGX010000000
VERSION     DASSGX010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 6467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6467
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761132"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..635)
                     /locus_tag="VFI12_11360"
     CDS             complement(45..635)
                     /locus_tag="VFI12_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7057054.1"
                     /translation="MRGTSIIRLVALLSVFGLFGAVANPARADKPEKVVTHDSFDDVF
                     CDIPVHVTVDGWSILHIQDYVIQADNPEVQDDFWIGVIQDHYDVTWTNEAGVTLTNTV
                     RQTVQEDSLVDNGDGTWTYTFTINGMPEFLRVENDHINKDVGRLTQQFVFYFGDLSTQ
                     ADDELLSATILGITGPHPTAESDFELFCQNVQKVLG"
     gene            complement(714..1304)
                     /locus_tag="VFI12_11365"
     CDS             complement(714..1304)
                     /locus_tag="VFI12_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7057055.1"
                     /translation="MRGTMLVRLSALLAVLGLLAAMANPAAAARPEKIDQTAGPFPDE
                     ICGIPVMTTVSGFTILHIQDVVIPSTGEGSDDFWIGVIQDHLIVTSTNADGDSLVNFV
                     QQTRHEDAIVDNGDGTWTYTYTIIGRPEVLKSGNKSLAVDVGTISFADTFYLGDLSTT
                     ADNSFVSSEITGITGPHPEAESDFVLFCQIVTDILG"
     gene            complement(1697..3346)
                     /locus_tag="VFI12_11370"
     CDS             complement(1697..3346)
                     /locus_tag="VFI12_11370"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET7057056.1"
                     /translation="MTDADSPNGTSSAASSPLPRRRYGPLPVSLARFIGRESEIAQLT
                     DLLHRPEVRLVTLTGPGGVGKTHLALRAAAFLADQFADGAAFIDLAPIRAAELVVPTL
                     VRRLSFRGPGSSPTRDHLVGVLRQQEMLLVIDNLEHVIEAGAELAVLLSACPFVRILA
                     TSRVRLHISGEHVMLLSPLPLPDPSPDASFANIANADAVRLFVDRAEAVRPGFALTPG
                     NALAVAELCHRLDGLPLAIELAAGQSDTSSPEELLVRWENHNLRLADGPRDMPTRHQA
                     LDAAIDWSYDLLSPDERRAFACLSVFSGSFTMTAALAVLGMQDDPESWTIGRVRALLA
                     KSLLTRSERQAGASLYRMLGIIRGYGLRRLEDEPDAIEIRHRHAEYFAGQAEQAAADL
                     HTTNAAEWLEQIDHEIPDMRAALVWSVEHDPVTAARVTSALVPIWTARGSLTEGRAWL
                     DRVLATDAGNDRSPGAYRPDARRHRPARPDGIRLTSRERQVLQLIVDGLSDKEIASAL
                     SISARTVSNHVSGMLAKLGVSSRTAAATLALRQGSLLKPAE"
     gene            3611..3961
                     /locus_tag="VFI12_11375"
     CDS             3611..3961
                     /locus_tag="VFI12_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7057057.1"
                     /translation="MRTLMAPNPNSQLDRANWFALDFDAQPARVIATSLVIVQVALFL
                     IGRAVDLATQGAVAYGFFATMLLFATLVFVDARSVRMPIRRAAFWAGAVTIGMIFGAI
                     PYARRRQSLRRDAR"
     gene            3988..4464
                     /locus_tag="VFI12_11380"
     CDS             3988..4464
                     /locus_tag="VFI12_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7057058.1"
                     /translation="MANHPLDAMPSTAYSPERRSAAAQASPYGGMGGIFLLWLAFGAL
                     MLFWPDTLMSLWDRFLDWTLILQVVVGVLLLPWVVATWVWQSDWSTLTRALVIGFLVI
                     GTMISFTPRKRGGGLRVEGTNGRTEPRIQWRGRCHLPSGPDRRGCTRTGVPDFARA"
     gene            complement(4659..5750)
                     /locus_tag="VFI12_11385"
     CDS             complement(4659..5750)
                     /locus_tag="VFI12_11385"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HET7057059.1"
                     /translation="MVSALARFAVVLALLAVFAAPARAGGMATVHLDAPPQDVVVNVP
                     FSIGFMVMQHDVTPVNPDRVVVSATHRETGGTYSVDATQDGPTGHFIAELTFPKAGSW
                     KWMITPEPFAGTSFESILVQDAAGSAPSDTAVAHPAGIRRGSCQAPGEAQFPLSDVIP
                     NGVVLDGTTDQKTGVVGADAGVPVAISTTTVDVTIAELLAEPHAIIVVKNAQDAGTFV
                     ACGNLSGRMWNGELVVGLQQTNTSSDVGIATLREDAGKTVVSLYMMVVAPEQASTGPE
                     VVVEITGSGGDWRFDPARVEVASGTTVVWVNTTETAHTVTGKDLSFEDSSPIAPGETF
                     RERFTDPGEYGYYCSPHPFMTGTVVVKAN"
     gene            complement(5934..>6467)
                     /locus_tag="VFI12_11390"
     CDS             complement(5934..>6467)
                     /locus_tag="VFI12_11390"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HET7057060.1"
                     /translation="GACFSFYFSAGGTVLGSWLGNQCDGSDGRNDGITHIVLDGVEGR
                     LVALEYLAPRGYVAGKKVAFTKERGTFKNVRLEQVAGGVSVKVTTYKGATTSKLPNAC
                     YGLYRAVGTTWEFMTVACDGWDGAADGVVRIVGVAAGTYRLYQTTTPAGYKMPAYVSV
                     TVGTTTKSVVVRTYPAP"
BASE COUNT         1096 a         2059 c         2225 g         1087 t
ORIGIN      
        1 cgaatctccc tgtcaaacat ggtgtcactc cgcgcgattg cgccttagcc gaggaccttc
       61 tggacgttct ggcagaagag ctcgaagtcg ctttcggccg tcgggtgcgg gccggtgatc
      121 ccgagaatgg tcgcgctcaa caactcgtca tcggcttgcg tcgagaggtc gccgaagtag
      181 aagacgaact gctgggtaag gcgcccgaca tccttattga tgtggtcgtt ctcgacccgc
      241 aggaactcgg gcatgccgtt gatggtgaag gtgtaggtcc acgtgccgtc accgttgtcg
      301 acgagggagt cttcctggac cgtctggcgc acggtattgg tgagcgtgac gcccgcctcg
      361 ttggtccagg tgacgtcgta atggtcctga atcacgccga tccagaagtc gtcctgaacc
      421 tcggggttat cggcctggat gacgtaatcc tgaatgtgca ggatcgacca gccgtcgact
      481 gtgacgtgaa cgggaatatc gcagaacaca tcgtcgaagc tgtcgtgggt gacgaccttc
      541 tccggcttgt ccgccctcgc ggggttcgcc accgcgccga acaggccgaa taccgagagc
      601 agggcaacga gtcgaatgat cgaggttcca cgcatggcac acctcccatt tgaaaacgca
      661 tcccgggaac gcgatgcagc cccgggacgc gaggacgaca ctggcactga acgtcagccc
      721 agaatgtcgg tcacgatctg gcagaagaga acgaagtcgc tctcggcctc cgggtggggt
      781 ccggtaatcc cggtgatctc gctgctgacg aaggagttgt cggcagttgt ggaaaggtcg
      841 ccgaggtaga acgtgtccgc aaaggagatc gtgcccacgt cgacggcgag gctcttgttc
      901 ccgctcttca ggacctcagg ccggccgatg atcgtgtagg tataggtcca ggtcccgtcg
      961 ccgttatcga cgatcgcgtc ttcgtggcgc gtttgctgaa cgaagttgac cagcgagtca
     1021 ccatcggcat tggtcgacgt aacaatcagg tggtcctgga tgacgccgat ccagaaatcg
     1081 tcggaacctt ctccggtcga tgggatcacg acatcctgaa tatgcaggat ggtgaacccg
     1141 gaaacggtcg tcatgaccgg tattccgcaa atttcgtcgg gaaaaggccc ggcagtctgg
     1201 tcgatctttt ccggccgtgc agccgcggcg ggattcgcca tggcggcaag cagaccgagg
     1261 acagccagca gcgcggacag tcgaaccagc atcgttcctc gcataacgct cctccttaca
     1321 tatccgcccc gaagccgacc gcaggcttcg aacgactaca acaaaacgag ccacaaacga
     1381 tgtacgcggg tcatgtcggg tcctttccca acgcacgggc gcctggatca acgcggatga
     1441 ggtggcgctg gggctcccgc gagcgggaga gcacggcccc agcgccggag acggctgtcg
     1501 cgtgatatca ggcagaggag gtcaggcggc ggggccggat cccggggtgc catggcggct
     1561 cgccaagtcg acctcggcgg ctccggtatg gcgccccacc ctctgcaacg tcaggatcgg
     1621 gaatcaccgt accgggcgaa gtgccgccgg gaaatcagga acattgcgca tttcggcgat
     1681 tgcggggtga gtaattttac tcagccggct tgagcagcga cccttggcgg agggccaatg
     1741 tggcggcggc tgtccgggag gagacaccga gcttggccag catcccgctg acatggttgc
     1801 tgacggtgcg ggcgctgatc gagagcgccg aggcgatctc cttgtcggag aggccgtcga
     1861 cgatcaactg caatacctgc cgctcccggg aggtcaggcg aatgccatcc gggcgagcgg
     1921 ggcggtggcg gcgcgcatcc ggccggtagg cgccggggga gcggtcgttg ccggcgtcgg
     1981 tggccagcac ccggtccagc caggcgcgac cctcagtgag cgagccgcgc gcggtccaga
     2041 tcgggaccag ggcgctggtc acgcgggcgg cggtcacggg gtcgtgctcg accgaccaga
     2101 cgagggcggc gcgcatatcc gggatttcgt ggtcgatctg ctccaaccac tcggcggcat
     2161 ttgtcgtgtg cagatcggct gccgcctgct ccgcctgccc ggcgaagtac tcggcgtggc
     2221 ggtgacggat ctcgatcgcg tccggctcat cctcgaggcg gcggagccca tatccccgga
     2281 tgatgcccag catccggtag agcgaggcgc cggcctggcg ctccgaccgg gtcagcaagc
     2341 tcttggcaag gagcgctctg acccggccga tggtccagga ctcgggatcg tcctgcatgc
     2401 ccagtacggc cagcgcggcc gtcatggtga acgagccgct gaagacggag aggcaggcaa
     2461 aggcgcgccg ttcgtcgggc gagagcaggt cgtagctcca atcgatcgcg gcatcgagcg
     2521 cctggtgccg ggtcggcatg tcgcgcgggc cgtcggccag gcgcaagttg tggttctccc
     2581 agcggaccag cagctcctcg ggcgacgagg tgtcggattg ccctgccgcg agctcgatgg
     2641 ccagcggtaa tccatccagg cggtgacaga gctcggcaac cgccagcgca ttcccgggcg
     2701 tgagcgcgaa acccgggcga acggcctcgg cgcgatcgac gaacaggcgc acggcgtccg
     2761 cgttggcgat gttcgcaaac gaagcatcgg gcgatgggtc aggcagcggc agcggcgaga
     2821 ggagcatcac atgctcgccg gagatatgca gccggacacg actggtggcc aggatcctga
     2881 cgaacggaca ggccgagagc agcaccgcga gttcggcccc cgcctcaatc acgtgctcca
     2941 ggttgtcgat cacgagcagc atctcctgct ggcgcagcac gccgaccagg tgatcgcggg
     3001 ttgggctgga gcccggaccg cggaagctca agcggcgcac cagcgtcggc accaccagct
     3061 ctgcggcccg gatcggggcg agatcgatga aggccgcgcc atcggcaaac tgatcggcca
     3121 ggaacgccgc ggcccgtaag gccaggtgcg tcttgccgac gccaccggga ccggtcaggg
     3181 tcacgagacg aacctccggg cgatgcagca ggtccgtgag ctgggcgatc tcggactcgc
     3241 gcccgatgaa ccgcgccaac gagacgggca agggcccata gcgccggcgc gggagtggcg
     3301 acgaggcagc ggaagacgtc ccgttcggcg agtcggcgtc ggtcatggcg ggctccgacg
     3361 gggtcgctgg agtcgagcct gtactatgcc actatactta tctacgtcaa ctgttcggcc
     3421 gcgcgaaaca gactctcggg accccctcgg gcggcaatct ctcgtattgc aatgaatctg
     3481 caatggtccg tggctgcaac cgggcgcctg tgtcatgccc ggcgggtcat gatgggcgtg
     3541 aggccgggag atgctgccag gcatcgggcc gcagccatga ccccggcgct gttcgtccag
     3601 gaggaccttc gtgcgcacgc tcatggcgcc aaatcccaac tcccagcttg atcgcgcgaa
     3661 ctggtttgcg ctcgatttcg atgcgcagcc ggcgcgggtg atcgcgacct cgctcgtcat
     3721 cgtccaggtt gcgctcttcc tgatcggccg ggcggttgat ctcgcgacgc agggcgcggt
     3781 ggcgtatgga ttcttcgcca cgatgctgct cttcgcgacg cttgttttcg tcgacgcgcg
     3841 ttccgtgcga atgccgatcc ggcgggcggc gttctgggcg ggggcagtca cgatcggcat
     3901 gatcttcggc gcgatcccgt atgcccgccg gcgccagtcc ctgcgccgcg acgctcgcta
     3961 ggccccgcac gcggaaagga gtccgcgatg gcaaatcatc cgctggacgc aatgccgtcc
     4021 accgcgtatt cgccggaacg ccggagcgca gccgcccagg ccagcccgta cggcggcatg
     4081 ggcggcattt tcctgctctg gctcgcgttc ggcgcgctga tgctcttctg gccggatacg
     4141 ttgatgagcc tgtgggatcg attcctcgac tggaccctga tcctccaagt cgttgtcggc
     4201 gtgctgctgt tgccgtgggt cgtagcgacc tgggtttggc aatcggactg gtcgacgctg
     4261 acccgtgcgc tggtgatcgg cttcctcgtg atcggcacga tgatcagctt caccccccgc
     4321 aagcggggtg gcgggttgag ggttgaggga acgaacggac ggacggaacc acggatccaa
     4381 tggaggggca gatgccatct gccctccggt cctgaccggc gcggttgcac gcggacgggc
     4441 gtgcccgatt tcgcccgggc gtaaacaccc gggctgaatc aaataagcca gctaaagcgg
     4501 gctgaatggg atgtggtttc gaaacgctgc gcctcatgac gtagcgcggt gcgatcagtg
     4561 cctgcgaacg ggccgggcgg ggagcgtcgg gcagatggta tctgcccctc catcgcctcc
     4621 tgcaactccg cgccttcgcg cctccccgcc ttcgcgcctc aattcgcctt gaccaccacc
     4681 gtgcccgtca tgaacgggtg cggcgagcag tagtagccat attcgccggg atcggtgaag
     4741 cgctcgcgga aggtctcgcc cggggcgatc ggactagagt cttcgaagga cagatccttt
     4801 ccggtcacgg tgtgggcggt ctcggtcgtg ttgacccaga caaccgtcgt gccggacgca
     4861 acctcgaccc gcgccgggtc gaagcgccag tccccgccgc tgccggtgat ctcgacgacc
     4921 acttcgggac cagtcgatgc ctgctcgggg gcgacgacca tcatgtagag cgagacgacc
     4981 gtcttcccgg catcctcgcg cagcgtggcg atgccgacgt cgctcgaggt gttcgtttgc
     5041 tggagcccga cgaccaattc gccattccac atccggccgc tgaggttgcc gcaggcgacg
     5101 aaggtgccgg cgtcctgcgc attcttcacg acgatgatcg cgtggggctc ggccaggagc
     5161 tcggcgatcg tgacatcgac cgtcgtggtg ctgatcgcga ccggaacgcc ggcatcggcg
     5221 ccgaccacgc ccgtcttctg gtcggtcgtg ccatccagca cgacgccgtt cgggatcacg
     5281 tcgctgagcg ggaactgggc ctcgcccggc gcctggcagc tcccccggcg gatgccggcg
     5341 gggtgcgcga ccgcggtgtc cgacggcgcg ctgccggcag catcctggac caggatcgat
     5401 tcgaacgagg tgccggcgaa cggctccggc gtgatcatcc acttccagct tccggccttc
     5461 gggaaggtca gctcggcgat gaagtgaccg gtcgggccgt cctgcgtggc gtcaaccgag
     5521 taggtcccgc cggtctcgcg gtgcgtggcg gagacgacga cgcggtcggg gttgaccggc
     5581 gtgacgtcat gctgcatgac catgaagccg atgctgaacg ggacgttgac gacgacatcc
     5641 tggggcggcg cgtcgaggtg gaccgtcgcc ataccgccgg cccgggcggg cgcggcgaaa
     5701 acggcgagca gggcgaggac gacggcgaac cgggcgagtg cggaaaccat gcggctaccc
     5761 tccattcggt tgcacggcga ccgcgccagg aagatcggcg cggcacacct ccaatacaac
     5821 cgccgcggag gatcggttcc gggttgaggg ctgagggaac cgcaatccgt cgccactgct
     5881 ccccgtgtcc aggcaacggg gagcagatgg cttgggatgg cgcggcggtc gggctacggt
     5941 gcggggtagg tgcggaccac caccgacttg gtcgtggtgc cgaccgtgac cgagacgtag
     6001 gccggcatct tgtaccccgc cggtgttgtc gtctgataca accggtaggt gccggccgca
     6061 acgccgacga tccggacgac gccgtccgca gctccgtccc agccgtcgca ggccaccgtc
     6121 ataaactccc aggtcgtgcc gactgcccgg tagagcccgt agcaggcgtt gggcagcttg
     6181 ctggtcgtgg cgcccttgta tgtggtgacc ttgaccgaca ccccgccggc cacctgctcg
     6241 aggcggacgt tcttgaaggt gcctcgctcc ttcgtgaatg ccaccttctt gccggcgaca
     6301 tagccgcgcg gagccaggta ctcgagcgcc accagccgcc cctccacgcc atcgagcacg
     6361 atgtgggtaa tgccgtcgtt gcgcccgtcg gagccatcgc actggttccc gagccacgag
     6421 cccagcaccg tcccgccggc cgagaagtag aagctgaagc aggcgcc
//
LOCUS       DASSGX010000629         4443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_216358, whole genome shotgun sequence.
ACCESSION   DASSGX010000629 DASSGX010000000
VERSION     DASSGX010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216358"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="VFI12_11395"
     CDS             <1..561
                     /locus_tag="VFI12_11395"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET7057061.1"
                     /translation="HDAVRMIRRSDLLVIDERHAGYGARSLLPLVREFDRVVVLRTFE
                     TWAGLSSLPVSYAIGPPAIVAALNAAILLRVGAGSLIAAHASLDDARSVNYAVARIKD
                     EKARMYRMLRKLNMVRPMPSWANFVLVRVERGDPSLYPDALLKNDIRVHIPEQPELEG
                     YLRISAVSHDATAALRAALIEIGAVV"
     gene            complement(859..1605)
                     /gene="rph"
                     /locus_tag="VFI12_11400"
     CDS             complement(859..1605)
                     /gene="rph"
                     /locus_tag="VFI12_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HET7057062.1"
                     /translation="MTTYVEPIRRSFDRTVEQARPITIQTGTMPAAEGSATIALGNTV
                     VLCAATVEERVPKWISQRGRGWVTAEYGMLPRATRERTRREAVEGKQGGRTVEIQRLI
                     GRSLRAIIDLDRLGERQIILDCDVLNADGGTRCASITGAYVAMALAIRKMIEQKRLRD
                     DPLRGQVAAISAGVVKGVPMLDLDYSEDAGADVDFNVVMTDAHAFVEIQGTAEGQPFM
                     PATLDEILVLASTGLDQLFAAQRQALMSAS"
     gene            complement(1602..2948)
                     /locus_tag="VFI12_11405"
     CDS             complement(1602..2948)
                     /locus_tag="VFI12_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HET7057063.1"
                     /translation="MTLDMFESRRRENLAASAPLATRMRPRTLDDLVGHADVVGPGTL
                     LRKAIDADRLSSIILWGPPGSGKTTLARIVAQTTRAHFTQLSAVTSGVADLRAAIKEA
                     ADRIGMTGQRTVLFIDEIHRFNKAQQDAILPHVEDGTVILIGATTENPSFEVNSPLLS
                     RSRVVVLHALTDADIGAIIRRSLTDEERGLGKEHLAITDDALDLLVNLANGDARFALN
                     TLEYASTGAGEGKITEELVAQAAQRRAATYDKSGDDHYDAISALHKTMRGSDPDAAMY
                     WLARMLERGDDPLYVARRVVRFASEDIGLADPQALTLAMAAQQAVHFIGMPEGALALA
                     EAVVYCALAPKSNALYRGYGAARTDVEQSRNDPVPIHLRNAPTKLMTDLGYGKGYRYA
                     HDFDEGVIGQQNLPDALAGRRYYQPTNRGLEADLRQRMERIRAIYEQTTPAEGTPE"
     gene            complement(2957..3148)
                     /locus_tag="VFI12_11410"
     CDS             complement(2957..3148)
                     /locus_tag="VFI12_11410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7057064.1"
                     /translation="MSKSGGTQKQLFDLYERLDRLEEMLEDMDEIGVSTRDQVEQMII
                     ALDQQIEELESAGAGDSTD"
     gene            complement(3163..4101)
                     /locus_tag="VFI12_11415"
     CDS             complement(3163..4101)
                     /locus_tag="VFI12_11415"
                     /inference="COORDINATES: protein motif:HMM:NF040134.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7057065.1"
                     /translation="MVSDFGEGITSQEQTISGERKDPRHDPATTGGTVLGSVHPPAYG
                     RGLLVSIVAAAIVFALAIFASGGKEEFTGIPMTQTTGSLFWGLAILAIILVAVGSQYA
                     EGVAIRVAESLGQEPARQDTLPSAWAVPAASIATALLLVATYHNRVMLVAGPAIAFLG
                     VAGGLLARDLLEDTMEGAERTAALIHTVVVHAIAFLGLSAVYMNKMSGWYGGPIVFML
                     TVVLMFEHLERANLSDGIRLLYASLSAGLMTLALLAVNWWPTYGWTGGAALLLCFFVL
                     AGFAAARGERQSLRDRDLIEYGAVFVGGLLILAITL"
     gene            complement(4165..>4443)
                     /locus_tag="VFI12_11420"
     CDS             complement(4165..>4443)
                     /locus_tag="VFI12_11420"
                     /inference="COORDINATES: protein motif:HMM:NF013468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HET7057066.1"
                     /translation="PNHLLAIELIERSGGAVACTSANISGKSAALSAREVLDTIGRSL
                     DGVVKGGVAPGGVPSTIIRITDGVVFLLREGPIPIEHIRATWDDFRTE"
BASE COUNT          732 a         1482 c         1476 g          753 t
ORIGIN      
        1 cacgatgcgg tccggatgat ccgccggtca gatctgctgg tgatcgatga gcgccatgcc
       61 gggtacgggg cgcggtcgct cctgccgctg gtgcgggagt tcgaccgggt cgtggtgctg
      121 cggacgttcg agacgtgggc cgggctgtcg tcgttgccgg tttcctacgc aatcggcccg
      181 ccggcgatcg tcgcggcgct caacgcagcg atcctgctgc gggtcggggc cggatcgctg
      241 atcgccgccc acgcttcgct cgacgacgca cggtcagtca actacgcggt cgcgcggatc
      301 aaggacgaga aggcgcgaat gtaccggatg ctgcgcaagt tgaacatggt ccggccgatg
      361 ccatcgtggg cgaacttcgt gctggtccgg gtcgagcgcg gcgacccatc gctctatccg
      421 gatgcgttgc tgaagaacga tatccgggtc cacatccctg agcagccgga gctggagggc
      481 tatttgcgga tcagcgccgt ctcccacgac gccaccgccg ccctccgcgc cgccctgatc
      541 gaaatcggcg ccgtggtcta gtccgattcg cccaattacc ccctgtaggc gtttgccgcc
      601 gccaactcgt ccttcgcacc atcaccggtt ggcatgcgcc cgccgatgta gggatttgcg
      661 ggggcaaatc cgtcggcgtt ccccgggccg ggtggatggg gtgaaccggc ggatgaggga
      721 cgttccggac acgaaccgtg ttcaccgggg aggcgcggcc gtgcccccgc acggccctac
      781 aacgggttcg tcgttgcccg gcaaggggcg ggtttacggc aacaaacccc gagcggcgtc
      841 cgggagtcga tcagcgcgtc aggaggcaga catcaaggcc tggcgctggg cggcgaagag
      901 ttggtcgagg ccggtgctgg cgaggacgag gatctcgtcg agcgtggccg gcatgaacgg
      961 ctgcccctca gccgttccct gaatctcgac aaaggcatgg gcgtcagtca tgaccacgtt
     1021 gaaatcgacg tccgcgccgg catcctcgct gtaatcgagg tcgagcatcg gcacgccctt
     1081 gacgacgccg gcgctgattg ccgcgacctg cccacgcagt ggatcgtccc gaagccgctt
     1141 ctgctcgatc attttccgga tcgcgagcgc cattgccacg taggccccgg tgatcgaggc
     1201 gcagcgggtg cctccgtccg cgttgaggac gtcacagtcg aggatgatct ggcgctcgcc
     1261 gagccggtcg aggtcgatga tcgcccgcag cgaccggccg atcagccgct ggatctcgac
     1321 cgtccgccca ccttgcttgc cctcgactgc ctcgcgccgg gtccgctccc tggtcgcgcg
     1381 cggcagcatc ccgtattcgg ccgtcaccca gccccgaccc cgctgcgaga tccacttcgg
     1441 cacccgctcc tcgaccgtgg cggcgcagag cacgacagtg ttgccgagcg cgatcgtggc
     1501 cgaaccctcg gccgcgggca tcgtgccggt ctggatggtg atgggccggg cctgctcgac
     1561 ggtgcgatcg aacgagcggc ggatcggctc gacgtaggtg gtcactcggg cgtcccttct
     1621 gcgggtgtgg tctgctcgta aatggcccgg atccgctcca tgcgctggcg gaggtcggct
     1681 tccaggccac gattcgtcgg ctgatagtat cgccgcccgg ccagggcgtc tggcaggttt
     1741 tgctggccga tcacgccctc gtcaaagtca tgcgcgtagc ggtagccctt gccgtaacca
     1801 aggtccgtca tgagctttgt tggagcgttg cggagatgga tcgggaccgg atcgttgcgc
     1861 gactgctcga cgtcggtccg cgccgcgccg tacccgcgat agagggcgtt gctcttcggg
     1921 gccagggcgc aatagacaac cgcctcggcc agcgcaagcg ctccctccgg catgccgatg
     1981 aaatggaccg cctgctgcgc cgccattgcc aacgtcaatg cctgcggatc ggccagcccg
     2041 atatcctccg acgcgaaccg gaccacgcgg cgggcgacat agagcgggtc gtctccgcgc
     2101 tccagcatcc gggcgagcca atacatggcc gcgtccggat cggagccgcg catcgtcttg
     2161 tgcagcgcag agatcgcatc gtaatggtcg tcgcccgact tgtcgtaggt cgcggcccgg
     2221 cgctgggcag cctgcgcgac cagctcctcg gtgatcttcc cctctcccgc gccggtcgag
     2281 gcgtactcga gcgtgttgag cgcaaaccgg gcatccccat tcgccaggtt gaccagcaag
     2341 tcgagcgcat cgtccgtgat cgccagatgc tccttgccca atccccgctc ttcgtcggtc
     2401 agggagcggc ggatgatcgc gccgatgtcc gcatcggtca gagcatgcag gacgacgacg
     2461 cgggagcggg agagcaacgg cgaattgact tcgaacgacg ggttctcggt cgtggcgccg
     2521 atcaggataa cggtgccgtc ctcgacgtgc gggaggatcg catcctgctg ggccttgttg
     2581 aagcgatgga tctcgtcgat gaagaggacg gttcgctgcc cggtcatccc gatccggtcg
     2641 gccgcctcct tgatcgccgc gcgcagatcg gccacgccgg aggtcaccgc gctgagctgg
     2701 gtgaagtgcg cccgggttgt ctgggcgacg atccgggcca gcgtcgtctt gccggagccg
     2761 ggcggccccc agaggatgat cgatgagagc cggtcggcat caatcgcctt ccggagaagt
     2821 gtgccggggc cgaccacatc ggcgtgaccg acgagatcgt cgagcgtgcg gggtcgcatc
     2881 cgggtggcaa gcggcgcact cgccgccagg ttttcccggc ggcgggattc gaacatgtcg
     2941 agggtcatgc ggtcactcag tcagtggaat cgccggcgcc cgccgattcc agctcctcga
     3001 tttgctggtc gagcgcgatg atcatctgct cgacctggtc ccgagtggag acaccaattt
     3061 cgtccatgtc ctcgagcatc tcctcgagtc gatccaaccg ctcgtagagg tcaaagagct
     3121 gcttttgcgt tccgccggat ttcgacacgg gaactcctgg agctacagcg tgatggcgag
     3181 gatgaggagc ccaccaacga agacggcgcc gtactcgatc aggtcgcggt cccgaagcga
     3241 ttgccgctcg ccccgcgctg ccgcaaagcc ggccagtacg aaaaagcaaa gcagcagggc
     3301 cgcgccgccg gtccacccat aggttggcca ccagttgacg gccagcagcg ccagcgtcat
     3361 caatccggcc gaaagggagg catacagcag gcgaattccg tcggacagat tcgcgcgctc
     3421 caggtgctcg aacatgagca ctaccgtcag catgaaaacg atcggcccgc cataccagcc
     3481 cgacatcttg ttcatgtaca ccgcgctcag gccgaggaag gcgatggcgt ggacgacgac
     3541 ggtatggatc agcgcggcag tccgctcggc gccctccatc gtgtcctcga gcaaatcgcg
     3601 cgcgagcaga ccaccggcca cgccgaggaa ggcgatcgcc ggcccggcca cgagcatcac
     3661 ccggttgtgg tacgtcgcga ccagcaacag cgccgtggca atggaggccg ccgggaccgc
     3721 ccacgccgac ggcaacgtgt cctgccgcgc aggctcctgg ccgagggatt ccgcgacccg
     3781 gatggcgaca ccttcggcgt actgcgaacc gaccgcgacc agaatgatcg cgaggatcgc
     3841 caacccccag aagagcgaac ccgtggtctg cgtcatcggg atgccggtga actcttcctt
     3901 gccgccggag gcgaagatgg ccagcgcgaa gacaatcgcg gccgcgacaa tgctgaccag
     3961 caagccacgc ccgtacgcag gcggatggac gctgccgagc accgtcccgc cggtcgtggc
     4021 agggtcgtgg cgcgggtcct tccgctcccc ggagatcgtc tgctcctgcg atgtgatgcc
     4081 ttccccgaaa tcggagacca tgcccaatcc tcctggcagc atcctactcc gttttcgcgg
     4141 cgaattcaca tgattgcgca gatttcattc ggtccggaag tcatcccacg tcgcgcggat
     4201 gtgctcgatc gggattggtc cctcccgcag caggaagacc acgccgtcgg tgatccggat
     4261 aatcgtcgac gggaccccgc ccggcgcgac cccaccctta acaacgccat cgagcgagcg
     4321 cccgatcgtg tcgaggactt ctcgcgcgct caatgcggca ctctttccgc tgatattggc
     4381 gctggtgcag gcgacggcgc ccccggatcg ctcgatgagc tcgattgcga ggagatggtt
     4441 cgg
//
LOCUS       DASSGX010000630         3726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1086889, whole genome shotgun sequence.
ACCESSION   DASSGX010000630 DASSGX010000000
VERSION     DASSGX010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 3726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3726
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1086889"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..911)
                     /locus_tag="VFI12_11425"
     CDS             complement(108..911)
                     /locus_tag="VFI12_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7057067.1"
                     /translation="MVNRGIYDHGWTACSKSILPWQATNEAITSTVDPFTAPWELCNI
                     DKDFSQAVDLAQQEPEKLQQMKDLFWAMAAKYDVLPLQWNTAGRLSGVRPFARPSLTR
                     GRTLLTYYPGMIRMQDNVAPNTYNRSFRITTEADIPESGANGALIALGGVEGGWSFTD
                     EDGKLVFHYNWVMASQYRVTSDTPLPTGKAQLVADVAYDGGMGKGATVTLSANGVQIG
                     EGRVDKTVPLLYGTDGFDIDGDYGSPVSPAYSSPFAFTGTLEKVTIDLL"
     gene            complement(1320..1472)
                     /locus_tag="VFI12_11430"
     CDS             complement(1320..1472)
                     /locus_tag="VFI12_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7057068.1"
                     /translation="MVEPQKPQERLQDRTEQKAQAQRNEERRERDDERDERKDERKDE
                     RADRKN"
     gene            complement(1779..3266)
                     /locus_tag="VFI12_11435"
     CDS             complement(1779..3266)
                     /locus_tag="VFI12_11435"
                     /inference="COORDINATES: protein motif:HMM:NF018561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HET7057069.1"
                     /translation="MSNAPTGIRLNPLSRRRLVQGVAAAGFGLAGSSMIAVMPSMART
                     LAQTPTANPITAAAVDAYLYGYSLITSEVTRVQQTNVDKIDLTTLRVPMGQFLNVPQY
                     PPADYQGITAPNADTLYSAAWLDVGDEPWLFSHPDMGDRFYLFPMYSLWMPVIDVPGT
                     RTAGGAAQTYAITGPGWTGDLPAGVKEIKSPTRYMYILGRTYCTGTEEDYAAVHKLQA
                     EYTVQPLSTYGQDYTPQAGTVDPNPPYSMTDKVRDVINAMAIEDYFSMMADLMGSSAP
                     PAAEDAEIVARMATFGLVPGQPFAPDAATKAALQDVGTLGYRQIDAYQSKSGTNQNGW
                     VIPEATGAYGADYLARAYVAAYGYPANLSADAVYPVAMTDGDGATLNGANAYTIHFEK
                     DQTPPANGFWSITMYDTGYFFYPNSLNKQTVSPRNDFVINDDGSLDLYFQHESPGADK
                     EANWLPAPAGDFILMMRLYWPKTEAPSILPPGHGTWTIPPVTKVG"
     gene            complement(3322..>3726)
                     /locus_tag="VFI12_11440"
     CDS             complement(3322..>3726)
                     /locus_tag="VFI12_11440"
                     /inference="COORDINATES: protein motif:HMM:NF018452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HET7057070.1"
                     /translation="GNSEEETIYVPYNTDAAGHTPDGSGAGYTLHFATGELPPVNAFW
                     SLTVYDAKTQLLVANPINRYLINSPMLPELTKDADGGLTLYLQHDEPPADHRANWLPL
                     PDGPFYAVLRMYWPQEAALSGEWQAPGLQPVR"
BASE COUNT          592 a         1159 c         1238 g          737 t
ORIGIN      
        1 gcgcgatcaa caggcgcgtg attgttgtat cggtcatggc ggtgacctcc tgctgccgtt
       61 cggcgctgct cctgatatcg ccagcacaat ccgccagctt cagaccccta cagcagatcg
      121 atcgtcacct tctccaacgt gccggtaaag gcgaacggcg acgagtaagc cggactgact
      181 ggcgatccgt agtccccgtc gatgtcgaaa ccgtcggtcc catagagcag cgggacggtc
      241 ttgtcgacgc gaccttcgcc gatttggacc ccattggcgg agagcgtgac ggtggcgcct
      301 ttgcccatgc cgccgtcata cgcgacgtcc gcgacgagct gcgctttccc cgtcgggagc
      361 ggcgtatcgg aggtgacccg gtattggctg gccatgaccc agttgtagtg gaagacgagc
      421 ttgccatcct cgtccgtgaa cgaccagccg ccttcgacgc cgcccagggc gatcagcgcg
      481 ccattcgcgc cgctctcggg gatgtccgcc tcggtggtaa tgcgaaacga ccggttatac
      541 gtgttcggcg cgacgttgtc ctgcatgcgg atcatcccgg gatagtaggt gagcaacgta
      601 cggccccgtg tgagactggg ccgcgcaaag gggcggacgc cggagagccg tcccgccgtg
      661 ttccattgca ggggcaggac atcgtacttc gcggccatgg cccagaacag atccttcatc
      721 tgctggagct tctcgggttc ctgctgcgcc agatcgaccg cctgcgagaa atccttgtcg
      781 atgttgcaga gctcccaggg cgcggtgaag ggatcgacgg tggaagtgat ggcttcgttg
      841 gtcgcctgcc aggggagaat ggacttcgag cacgccgtcc agccgtgatc gtaaatgccg
      901 cggttgacca tgatctcgaa gtactgctgg gtgcgccggc cggggggcgc gtcgtgcgcc
      961 gggtgagagc ggagggaggc gccggtgcgg gtgcatcctg tgatgactcg atgctcgggg
     1021 tgtcgtccac ggttgtgttc cccttgacga cttccggacg aaaatcgaca ttgcgttgcc
     1081 gcatccttga ccgcgcgctt cccttgtcac ccgcgcgact tcaccgttca tctcccggtg
     1141 gtgactcagc gcgttgcccg gcgccgttgg cgcgcgggcg gcctctgcgt aagggcggtc
     1201 tccgtggggc gacgcctgcg tcgtcccacg gagattctca acgttgggca actgcctccg
     1261 tagggtgctc gggcagaggt gagcacgccg ccggagccgg ccgcctgaga gcatgagcgt
     1321 tagttcttgc ggtcggcccg ctcgtcctta cgctcgtcct tgcgctcgtc gcgctcgtcg
     1381 tcgcgctctc gccgctcctc gttccgctgg gcctgggctt tctgctcggt gcggtcctgc
     1441 aagcgctctt gcggtttctg tggttcaacc atcgtctcac gctcctctgc gataaaccta
     1501 tgctcccccg tgtggagcat gaactccggg gttcagccat cggcgcacga cttcgggatt
     1561 ggcggttggc caggcgccag agcatgcgat tgggcacatg ctccggcgac cagcgccact
     1621 ctgcccgcgc gatcccttcg cctcgctgcc gtgtgaccga tccttccgcc gtcatcagca
     1681 cgggttcgtt ctgatacggc gattccaatg tggaaccaac atgagtgacg tttggcgccg
     1741 gcgcgacaac cttcccttcg ctctatggcg ctggctcctc aacccacctt cgtcacgggc
     1801 gggatcgtcc acgtgccatg cccggggggc aggatcgacg gggcctcggt cttcggccag
     1861 tagagccgca tcatcaggat gaagtcgcct gctggcgctg gcagccagtt cgcctccttg
     1921 tcagcccccg gcgactcgtg ctggaaatag agatcgagcg agccgtcgtc gttgatgacg
     1981 aaatcgttgc gcgggctgac cgtctgcttg ttcagcgagt tgggatagaa gaagtaaccg
     2041 gtgtcgtaca tcgtgatcga ccagaagcca ttggccggcg gcgtctggtc tttctcgaaa
     2101 tggatcgtgt aggcgttggc tccgttcaac gtcgccccgt cgccgtcggt catcgcgacg
     2161 ggatagacgg cgtccgcgga gaggttggcc ggatagccat aggccgccac gtaggcgcgg
     2221 gccaggtagt cggccccata ggcgccggtt gcctcgggga tgacccagcc attctggttc
     2281 gtgccagact tgctctggta ggcgtcgatc tgccggtacc ccagcgttcc gacatcttgc
     2341 agagccgcct tggtcgccgc atcgggcgca aacggctggc cagggacaag cccaaacgtg
     2401 gccatccggg ccacgatctc ggcatcctcc gccgccgggg gcgcggaact gcccatcagg
     2461 tccgccatca tactgaaata gtcttcgatc gccatggcgt tgatgacgtc gcggaccttg
     2521 tcggtcatgc tgtagggtgg gtttgggtcg accgtcccag cctggggcgt atagtcctgc
     2581 ccgtacgtgc tgagcggctg gaccgtgtac tcggcctgga gcttgtggac ggcggcgtag
     2641 tcttcctccg tgccggtgca atacgtgcgg cccaggatgt acatatagcg cgtgggcgac
     2701 ttgatctcct tgaccccagc cggcagatca cctgtccacc ccggcccggt gatggcatag
     2761 gtctgcgccg cgccgccggc cgtgcgcgtg ccgggcacgt cgatgaccgg catccacagg
     2821 ctgtacatgg ggaagaggta gaagcggtct cccatgtccg gatgggagaa gagccagggc
     2881 tcatctccga catcgagcca ggccgcggaa tagagggtgt ctgcattcgg agcggtgatg
     2941 ccctggtagt ccgccggggg gtactgggga acattgagga actggcccat cgggacgcgc
     3001 aacgtcgtga gatcgatctt gtcgacgttc gtctgctgca cgcgggtcac ttctgaggta
     3061 atcagggagt acccatagag atacgcatcg accgcggcgg cggtgatggg gttggccgtc
     3121 ggggtttgcg ccagggtccg cgccatactg ggcatgaccg caatcatgct ggaaccggcg
     3181 aggccgaagc ccgccgcggc aaccccctgc accaaacggc ggcgagacag cggattcagg
     3241 cgaattccgg tgggtgcgtt agacaacgat gtcctccgag gcttcatggc aaatcctccg
     3301 cttgcaacgg attgccgaat gtcaccgtac cggttgcaac cccggcgctt gccactcgcc
     3361 gctcagcgcc gcctcctgcg gccagtacat gcgcagaaca gcgtaaaagg gaccgtcggg
     3421 aaggggcagc cagttggctc ggtgatccgc gggcggttcg tcgtgctgca ggtagagcgt
     3481 caacccgccg tcggcgtcct tggtcagctc gggcagcatc ggcgagttga tcaggtaccg
     3541 gttgatcgga ttggcgacca gcagttgcgt cttggcgtcg tagacagtca aagaccagaa
     3601 ggcgttgacg ggcggcaact cccctgtcgc gaaatgcagt gtgtagccgg cgccgctgcc
     3661 atccggtgtg tggccggccg cgtcagtgtt gtacgggacg tagatcgtct cttcctcaga
     3721 attgcc
//
LOCUS       DASSGX010000631         2338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_652992, whole genome shotgun sequence.
ACCESSION   DASSGX010000631 DASSGX010000000
VERSION     DASSGX010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_652992"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="VFI12_11445"
     CDS             <1..586
                     /locus_tag="VFI12_11445"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HET7057071.1"
                     /translation="DIERVRAASKAAGKAKLAIDANNAWTAPVALKFMRAISDIEIAW
                     LEEPVPTEDLAGSAEIAHILDTPVAGYETETGLPGFRNLITHRAVDIVQPDVIWTGGI
                     TETRKVAALAQAFHLPVIPHVFSSAVSSIANMHLIASLPNAGLLEFDQNPNPLRSELF
                     EEPITVDENGQVRLPDAPGLGVRLNQKTVDKYRI"
     gene            609..1388
                     /locus_tag="VFI12_11450"
     CDS             609..1388
                     /locus_tag="VFI12_11450"
                     /EC_number="1.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005472.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-iditol 2-dehydrogenase"
                     /protein_id="HET7057072.1"
                     /translation="MRLEGKTAIVTGGAQGIGRACARRFAGEGAKVVIGDLQEDGAKA
                     VAKEIADAGGQAVGVALDVRDQQQAQAFVDRAVAEFGSVDILMNNAGVIRITPFLEIT
                     EEEWDLLFDVNCKGMLWCSQAAARQMIKQGRGGKIINVASQAGRRGEALVLTYCASKA
                     AVISMTQSMGLALAEHKINVNAIAPGIVDTPLWESNDRRFAELLGMEIGEPKRTFVAS
                     IPLGRIEQPEDVAGMAAFLASPDSDYITQQTYNVDGGNWPS"
     gene            complement(1499..>2338)
                     /locus_tag="VFI12_11455"
     CDS             complement(1499..>2338)
                     /locus_tag="VFI12_11455"
                     /inference="COORDINATES: protein motif:HMM:NF016104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000009 - alpha-1,6-mannosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004376 - glycolipid mannosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase family protein"
                     /protein_id="HET7057073.1"
                     /translation="SNAGLAVIGISLSTVMFLGATALLARLSIDFLEYDEARFAVMLF
                     AVAPFAFFYTAAYTESLFVLEVISAVWFARRGQWWRAAIMAALASATRLVGLAVIGGV
                     LYAAWRSRKPLPDLVGMAAVGASGFVAFMLYLWVKFDNPTAYFDTQARWGGWSEHVWF
                     YVKLFLTRPREALSGDPRHLIIIHNVALGLIALALVPIIIRRLDPTAAGISVLLIVGQ
                     FLITWVSLGRYLMPAIGIYFAMALITSRTGRYSWARETILALSLISMTALALLYAHGF
                     WVV"
BASE COUNT          463 a          759 c          790 g          326 t
ORIGIN      
        1 ggatatcgag cgcgtccggg cagcgagcaa ggcagccggg aaggcgaagc tcgcgatcga
       61 cgccaacaat gcctggaccg cgccggtcgc gctcaaattc atgcgcgcga tcagcgacat
      121 cgagatcgcc tggctcgagg agccggttcc aaccgaggac ctggccggca gcgccgaaat
      181 cgcgcacatc cttgacacgc cggtcgccgg ctacgagacc gagaccggcc tgcccggttt
      241 ccgcaacctg atcacgcacc gggcggtcga catcgtccaa ccggacgtaa tctggaccgg
      301 cggcatcact gagacgcgga aggtcgcggc cctggcccag gcgttccacc tgccggtgat
      361 cccgcacgtc ttctcgtcgg cggtcagttc gatcgccaac atgcacctga tcgcctcgct
      421 cccgaacgcc ggcctgctcg agttcgacca gaacccgaac ccgctgcgct cggagttgtt
      481 cgaggagccg attacggtcg acgagaacgg gcaggtccgg ctgccggacg cgccggggct
      541 cggcgtccgc ttgaaccaga agaccgtcga caaataccgg atctgaggtc agcgaaatgg
      601 aggagactat gcgactcgag ggcaagactg cgatcgtgac cggcggcgcg caggggatcg
      661 gccgggcgtg cgccaggcgc ttcgccggcg agggcgcaaa ggtcgtcatc ggtgatttgc
      721 aggaggacgg cgcgaaggcg gttgcaaagg agattgccga cgccggcggc caggcggtcg
      781 gggtggcgct cgacgtgcgg gaccagcaac aggcgcaggc cttcgtcgat cgggcggttg
      841 ccgagtttgg cagcgtcgac atcctgatga acaacgccgg cgtgatccgg atcacgccct
      901 tccttgagat cacggaagag gagtgggacc tgcttttcga cgtcaactgc aagggcatgc
      961 tctggtgctc gcaggcggcg gcccggcaga tgatcaagca agggcgcggc ggcaagatca
     1021 tcaatgtcgc ctcgcaggcg ggccgtcgcg gggaggcgct ggtcctgacc tactgcgcga
     1081 gcaaggcggc ggtgatcagc atgacgcagt cgatggggtt ggcccttgcc gagcacaaga
     1141 tcaacgtcaa tgcgatcgcg ccgggcatcg tcgacacgcc actctgggag tcgaacgacc
     1201 gccgcttcgc ggaactgctc ggcatggaga tcggcgagcc caagcgaacc ttcgtggcca
     1261 gcatcccgct cgggcggatc gagcagccgg aggacgtcgc cggaatggcc gccttcctcg
     1321 cctcacccga ctccgactac atcacccagc aaacctacaa cgtcgacggc ggcaactggc
     1381 cgagctagga gcccgtaggg gcggggcacc gcacctttgg gtcggtcaac gccgactcct
     1441 tggcaagcaa ggtcaccttc aacgtagcgc ggtgtccccg cgccctgttc cgtcgccatc
     1501 acacgaccca gaagccgtgg gcgtagagga gggcaagggc agtcattgag atcagggaga
     1561 gggcgagtat cgtctcgcgc gcccagctat accggccggt ccgcgacgtg atcagcgcca
     1621 tcgcgaagta gatgccgatc gccggcatca ggtagcggcc gagtgaaacc cacgtgatca
     1681 ggaactggcc cacgatcagc agcaccgaga tcccggccgc agtcggatcg agccggcgga
     1741 tgatgatcgg gaccagcgcc agcgcgatca ggccaagcgc gacgttgtgg atgatgatca
     1801 gatggcgcgg atcgccgctc agggcctcac ggggccgggt cagaaaaagc ttgacgtaga
     1861 accagacgtg ctcgctccag ccgccccagc gcgcctgggt atcgaaatag gccgtcggat
     1921 tgtcgaactt cacccacagg tagagcatga aggcgacgaa gccgctcgcg ccgaccgccg
     1981 ccatcccgac caggtcgggt agcggtttcc tgctccgcca ggcagcgtag agcacgccgc
     2041 cgatcaccgc caatccgacc aggcgcgttg ccgaggcaag cgcggccatg atcgccgccc
     2101 gccaccattg gccacgccgc gcgaaccaga ccgccgagat gacctcgagc acgaagagcg
     2161 attcggtgta ggccgcggtg tagaagaagg cgaacggcgc gacagcgaac agcatgacgg
     2221 cgaaccgcgc ctcgtcgtat tcgaggaaat cgatcgacag ccgggccagc agcgcggtcg
     2281 cgcccaggaa catgaccgta ctgagtgaga tgccgatcac cgccaggccc gcattcga
//
LOCUS       DASSGX010000632         2964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Thermomicrobiales bacterium isolate SMAG_U1044
            k141_1087587, whole genome shotgun sequence.
ACCESSION   DASSGX010000632 DASSGX010000000
VERSION     DASSGX010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963209
            Sequence Read Archive: SRR25158201
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Thermomicrobiales bacterium (soil metagenome)
  ORGANISM  Thermomicrobiales bacterium
            Bacteria; Thermomicrobiota; Thermomicrobia; Thermomicrobiales.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/10/2024 03:21:55
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,294
            CDSs (total)                      :: 2,277
            Genes (coding)                    :: 2,271
            CDSs (with protein)               :: 2,271
            Genes (RNA)                       :: 17
            tRNAs                             :: 15
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 6
            CDSs (without protein)            :: 6
            Pseudo Genes (ambiguous residues) :: 0 of 6
            Pseudo Genes (frameshifted)       :: 1 of 6
            Pseudo Genes (incomplete)         :: 4 of 6
            Pseudo Genes (internal stop)      :: 3 of 6
            Pseudo Genes (multiple problems)  :: 1 of 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Thermomicrobiales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087587"
                     /isolate="SMAG_U1044"
                     /isolation_source="soil"
                     /db_xref="taxon:2482790"
                     /environmental_sample
                     /country="China"
                     /lat_lon="45.0125 N 124.9825 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="VFI12_11460"
     CDS             complement(<1..613)
                     /locus_tag="VFI12_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7057074.1"
                     /translation="MMTRQSNIAAVLEDDREPPERDPDRASQSAPVRASLVPRQAFLV
                     GPTVYLRPIELGDAQIATFWRPSPFPTPADLVEEQLKDSLAREVASGTRRLIACRRSD
                     NQPIGSAQIHSDDGRTSMLAFHVPGVFGPQRASEVTAEMIRLLVPWLIHEHDQMVVWV
                     QIPADDPTLTRAVRVAGMKHAYHLREALQTADGDRHDLECWQAL"
     gene            850..1698
                     /locus_tag="VFI12_11465"
     CDS             850..1698
                     /locus_tag="VFI12_11465"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HET7057075.1"
                     /translation="MRANHAKRRLQAGEPSIGTWLSLPSPEAAEYVADLGFDWLTVDA
                     EHNAIDITSMSRMFGVIAAAGTAPLVRIPWNTAENIKRVLDAGAWGIVVPMVNSRAEA
                     EAAVSAAKYPPRGTRSLGGGMRAIRWGASADDYSRNADDEILVVLQIEHIDGVNVADE
                     ILSVPGVDACFIGPNDLAASMGISLGIPLESDHPELVAAVEEIKAACLRNGVAPGIHC
                     SGPAGINQRIAEGFRFLAMASELKFLLSGLQDGLRAVEWTQSPRTRVGQVPAEASAPS
                     GDAVRY"
     gene            1771..2901
                     /locus_tag="VFI12_11470"
     CDS             1771..2901
                     /locus_tag="VFI12_11470"
                     /inference="COORDINATES: protein motif:HMM:NF039815.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET7057076.1"
                     /translation="MTVVRHWLAAALVVAFLTTMGAGSIALAQDQGTPGIEATRTARV
                     QPQPTRTPDTSGSDVAQTDDTSIDGGSTADEPSAPSPNAADIDISGMDQTIAQGLAAF
                     DDFSGGIWRITELKPLPVDQAPSVTAPYYGFIYQMKGNTIIRNNVSGKRARIEPGEAY
                     FFSAGDSYTRYQEDADGSRAWLIEVVPGTADDADAAGTVLWKSDEIGGFPDDTRDFEL
                     VAANILDGGSARVPDYEVDVLLMVTVGSIQVTDDNGTVRMDAPAALMVSGKVDIENTS
                     QDPANYLIGKIGSSVGDALPAVDDSTTPDESADTPDATSGDTTGEAADPELDTDGDGL
                     IDTDEAVYGTDPTIADTDFDGYSDGDEVMVYGTDPLDANSWP"
BASE COUNT          516 a         1023 c          927 g          498 t
ORIGIN      
        1 gcagcgcctg ccagcactcg aggtcgtgcc ggtcgccatc cgccgtctgg agcgcctctc
       61 gcaggtgata ggcatgcttc atcccggcta ctcgcaccgc ccgggtcagg gtcggatcgt
      121 cggccggaat ctgcacccag accaccatct ggtcgtgctc gtggatcagc cacggtacga
      181 gcagtcggat catctcggcc gttacctcgg acgcccgctg tggtccgaat actcccggca
      241 cgtggaacgc cagcatcgag gtccggccgt cgtccgagtg gatttgcgcc gacccgatcg
      301 gctggttgtc ggagcggcgg caggcgatca gccgccgtgt cccgctcgcg acttcccgcg
      361 ccagcgagtc cttcagctgc tcctcgacca ggtcggccgg tgttgggaag ggtgatggcc
      421 gccagaacgt ggcgatctgc gcgtcgccga gctcgatcgg gcgcaggtag acagtggggc
      481 cgaccaggaa cgcctggcgc ggaacgaggc tggccctcac cggagccgat tggcttgcac
      541 ggtcagggtc acgctcgggg ggctcacggt cgtcctccag gacggcggca atgttggact
      601 gtctggtcat cataggtgct cccggatctc gtattttatc gtactccgag agtgatatgg
      661 gatgacacct gtccagcatg acgctgatga actggaaagc gagatcacga ctcggaagtt
      721 ttgatcgtcg tggcgatatc ctatggcatg gctgcccatg gccaagaaca gtttgctacg
      781 ttgacggaga ttgtgggaca aacgttcgta accgcggcaa catttatccg cgcaggaagg
      841 aatggatcag tgagagcaaa ccacgcgaaa cgacgcctgc aggccggtga gccgtcgatc
      901 ggcacctggc tctcattgcc cagtcccgaa gcggccgagt acgtcgcgga tctcggcttc
      961 gactggctga cggtcgacgc tgagcacaat gccatcgaca tcacgtccat gtcccgcatg
     1021 ttcggcgtga tcgccgcggc gggcactgcc ccgttggtcc gcattccctg gaacacggcc
     1081 gagaacatca agcgcgtcct cgatgccggc gcgtggggca tcgtcgtgcc gatggtcaac
     1141 agccgggccg aggctgaagc cgccgtctcc gccgcgaagt acccgccgcg cggcacccgc
     1201 agcctcggcg gtggcatgcg cgcgatccgc tggggcgcct cggccgacga ctactcccgc
     1261 aacgccgatg acgagatcct cgtcgtcctc cagatcgagc acatcgacgg cgtcaacgtc
     1321 gccgacgaga tcctgagcgt gccaggtgtc gacgcctgct tcatcggccc aaacgacctg
     1381 gccgcctcga tgggcatcag cctcggcatt cccctcgaga gcgaccatcc cgagctggtt
     1441 gccgctgtcg aggagatcaa ggccgcctgc ctccgcaatg gcgtcgcgcc cggcatccac
     1501 tgctcgggtc cggccggaat caaccagcgg atcgccgaag gcttccggtt ccttgctatg
     1561 gccagcgagc tgaaattcct cctctcgggc ttgcaggacg gcctccgggc agtagaatgg
     1621 acacagtccc cccgcacccg agtcggccag gtcccggcgg aagccagtgc gccgtccgga
     1681 gacgccgtcc gctattagcg cgtaccattt gcgccgcgct tttgtgcctg ttagcgccac
     1741 cacccagcgc ggcggaggga atcgttttca atgaccgtag ttcgacactg gctggccgcg
     1801 gcgcttgtcg ttgccttttt gacaacgatg ggcgccgggt cgatcgcgct cgcccaggat
     1861 cagggcaccc ccggcatcga ggccacccgc accgctcgcg tccaacccca gccaacccgg
     1921 actccggata cctccggatc ggatgtcgcc cagactgacg acacctctat cgacggggga
     1981 agcaccgccg acgagccgtc cgcgccctct ccgaatgccg ccgacatcga tatctccggc
     2041 atggaccaga cgattgccca gggactcgcc gcatttgacg acttcagcgg cggcatctgg
     2101 cgcatcaccg agctcaagcc gctcccggtc gaccaggctc cgtccgtcac cgcgccctac
     2161 tacggcttca tctatcagat gaagggcaat acgatcatcc gcaacaacgt ctccggcaaa
     2221 cgcgcccgca tcgagcccgg tgaggcctac ttcttctccg ccggcgactc ctacacccga
     2281 taccaggagg acgcggacgg ctcccgcgcc tggctgatcg aggtcgtccc cggcacggcg
     2341 gacgacgccg atgccgccgg gacggtcctc tggaagtcgg atgagatcgg cggtttcccg
     2401 gacgacacgc gcgactttga gctggtggcg gccaacatcc tcgatggtgg ttcagcccgc
     2461 gtgccggact acgaggtcga tgtcttgctc atggtcaccg tcggctcgat ccaggtcacc
     2521 gacgacaacg gcaccgtccg gatggacgca cccgccgcgc tgatggtctc cggcaaggtc
     2581 gacatcgaga acacgagcca ggacccggcc aactacctga tcggcaagat cggctcctcg
     2641 gtcggtgacg cgctcccggc cgtcgacgac agcacgacgc ccgatgaatc cgccgatact
     2701 ccggacgcga cctcgggcga cacgaccggc gaggcagccg atcccgagct cgataccgac
     2761 ggcgacggcc tgatcgacac cgacgaagcg gtctacggca ccgacccgac catcgccgat
     2821 accgatttcg acggctactc tgatggcgac gaggtcatgg tgtacggcac cgatccgctc
     2881 gacgccaact cctggccgta gcacgtggtg ggcgagcgag acaaccggtt ggtgcaatga
     2941 ctgcgccgcc gctcgtcctc ttcg
//
